CN101011052A - Use of neomycin or salt thereof in pesticide - Google Patents

Abstract

The invention relates to an application of mycifradin or relative salt in the pesticide. The invention researches the mycifradin and relative salt on the disinfection activities of plant se-producing germs, to prove the high restrain functions, and high disinfect activity on the pest. The invention is stable and harmless with high efficiency.

Description

Neomycin or the purposes of its salt in agricultural chemicals

Technical field

The present invention relates to neomycin or the purposes of its salt in agricultural chemicals, belong to pesticide field.

Background technology

Biological control is one of important content of the comprehensive regulation, and " organic farming ", " ecological agriculture " and environmental protection come into one's own day by day.At present, biological control has become an important technique measure in the crop pest integrated controls such as paddy rice, wheat, corn, cotton, fruit tree, vegetables.Environment compatibility is good, safety, low-residual, economic biopesticide are the emphasis of research and development.

Neomycin is the antibiotic of separating from streptomyces streptomyces fradiae bacterial strain, belongs to the aminoglycoside broad-spectrum antibiotic, and it mainly is made up of actiline and Neomycin C, and eliminant is as follows:

Neomycin B：R ₁＝CH ₂NH ₂ R ₂＝H

Neomycin C：R ₁＝H R ₂＝CH ₂NH ₂

Neomycin (neomycin) structural formula

To G ⁺, G ^-Bacterium all has the strong antibiotic effect, and actinomycetes, Leptospira are also had certain effect.Clinically, be applicable to intestines and stomach and respiratory tract infection, gram-negative bacteria, positive bacteria and tubercle bacillus etc. are all had better effect.The most responsive with Escherichia coli, responsive to golden Portugal bacterium, bacillus anthracis, diphtheria bacillus, aerobacteria, proteus and shigella dysenteriae etc., more insensitive to Pseudomonas aeruginosa; Be usually used in preparing and local application before the colonic operation; Veterinarily, be one of choice drug of treatment fowl bacterial enteritis, effect spy is good; In addition, China and some other country also add neomycin in the animal feed to, and prevention is caused by bacterial infection morning, wall losses and enteritis improved efficiency of feed utilization, promoted growth, therefore were widely used in livestock breeding again.

Do not see at present neomycin as agricultural chemicals carry out control of plant disease and agricultural insect pests control application.

Summary of the invention

The object of the present invention is to provide neomycin or its salt purposes at pesticide field.

Described salt can be the sulphate of neomycin.

Further, the invention provides neomycin or its salt purposes in controlling plant diseases.

Wherein said plant disease is the plant disease that bacterium, fungi, virus cause, and the plant disease that causes of the pest of Insecta.Described phytopathy comprises the disease of cereal crops, economic crops or medicinal plant.

Further, described plant disease is paddy rice, wheat, corn, tobacco, peanut, potato, Chinese cabbage, tomato, eggplant, the disease that the bacterium of cucumber, green onion, ginger, konjaku, wild cabbage, capsicum, citrus, pears, apple etc., fungi, virus or pest (such as cabbage caterpillar, cotten aphid, locust etc.) cause.

The present invention also provides a kind of agricultural chemicals, it is characterized in that: it is to be pesticide activity component with neomycin or its salt, adds that the acceptable auxiliary element is prepared from the agricultural chemicals.

The content of neomycin or its salt can be 5-50% in the described agricultural chemicals, during use it is diluted to the 5-4000mg/L concentration range and gets final product.

The inventor studies various plants pathogen and pest activity neomycin and salt thereof, evidence neomycin and salt pair phytopathogen thereof have the good restraining effect, pest cabbage caterpillar, aphid, locust etc. are had contact toxicity preferably, provide a kind of new thinking for the neomycin exploitation becomes a kind of new environmental friendliness agricultural chemicals.

Below, foregoing of the present invention is described in further detail again by embodiment.But this should be interpreted as it is limitation of the present invention.

Embodiment:

The neomycin of following EXPERIMENTAL EXAMPLE derives from Sichuan University and Chengdu Pu Hui biotechnology Co., Ltd, the test plant pathogenetic bacteria derives from Sichuan University, Chinese common micro-organisms DSMZ, Chengdu Inst. of Biology, Chinese Academy of Sciences and Sichuan Academy of Agricultural Sciences, adopts to Sichuan University agricultural chemicals laboratory experiment field for the examination pest.Test example 1 neomycin suppresses the mensuration of plant pathogenetic bacteria, fungi activity

1 test plant pathogenetic bacteria

The test bacterial classification 1.1 exsomatize:

Xanthomonas oryzae (Xanthomonas oryzae pv.oryzae), paddy rice basal stem rot bacterium (Erwiniachrysanthemi pv.zeae), rice bacterial stripe bacterium (Xanthomonas oryzae pv.oryzicola), cotton angular leaf spot fungus (Xanthomonas campestris pv.malvacearum), corn bacterial wilt (Pantoeastewartii subsp.stewartii), tobacco ralstonia solanacearum (Pseudomonas solanacearum pv.tabaci), the bacillary prairie fire germ of tobacco (Pseudomonas syringae pv.tabaci), tobacco hollw stalk bacterium (Erwinia carotovorasubsp.carotovora), tobacco bacterial leaf spot bacterium (Xanthomonas campestris pv.vesicateria), bacterial blight of soybean bacterium (Pseudomonas syringae pv.glycinea), soybean macula germ

(Xanthomonasaxonopodis pv.glycines), bacterial wilt of peanut bacterium (Ralstonia solanacearum), potato bacterial wilt bacterium (Ralstonia solanacearum), potato rot positive germ (Erwinia carotovora subsp.carotovora), potato bacterial ring rot bacterium (Lavibacter michiganensis subsp.sepedonicus), soft rot of cabbage bacterium (Erwinia carotovora pv.carotovora), Chinese cabbage black rot (Xanthomonas campestris pv.campestris), bacterial wilt of tomato bacterium (Ralstonia solanacearum), bacterial canker of tomato (Clavibactermichiganense subsp.michiganense), ginger ginger plague germ (Ralstonia solanacearum), cucumber bacterial angular leaf spot bacterium (Pseudomonas syringae pv.lachrymans), konjak soft rot bacterium (Erwinia carotovorapv.carotovora), cabbage black rot bacterium (Xanthomonas campestris pv.Campestris), Chinese cabbage alternaria (Alternaria brassicae), pepper scab fungus (Xanthomonas vesicatoria), candidatus liberobacter asiaticum (Liberobacter asiaticum), c itrus canker germ (Xanthomonas axonopodis pv.citril), apple crown gall germ (Agrobacterium tumefaciens).

1.2 for examination pathogeny fungi and pot plant

The test bacterial classification 1.2.1 exsomatize:

Pyricularia oryzae (Piricularia oryzae Cav), fusarium moniliforme (Fusarium monilifome), Rhizoctonia solani Kuhn (Rhizoctonia solani), fusarium graminearum (Fusarium graminearum), root rotof flax bacterium (Bipolaris sorokinianum), rhizoctonia cerealis (Rhizoctonia cerealis), gaeumannomyces graminis (Gaeumannomyces graminis), Exserohilum turcicum (Exserohilum turcicum), corn is bent spore leaf spot fungi (Curvularia lunata), southern corn leaf blight (Bipolaris maydis), cotton anthracnose bacterium (Colletotrichumgossypii), cotton rhizoctonia solani (Thanatephorus cucumeris), cotton-wilt fusarium (Fusarium osysporu f.sp.vasinfectum), verticillium dahliae (Verticillium dahliae), Sclerotinia sclerotiorum (Sclerotiniasclerotiorum), peanut Cercospora bacteria (Cercosporaarachidicola), cercospora leaf spot of groundnut bacterium

(Phaeoisariopsispersonata), sweet potato pine root fungus (fusarium solani), phytophthora infestans (Phytophthora infestans), soybean anthracnose bacterium (Colletotrichum truncatum), anthracnose of tobacco bacterium (Colletotrichum nicotianae), tobacco black shank bacterium (Phytophthora nicotianae), tobacco brown spot pathogen (Alternaria alternata), tea zonate spot bacterium (Pestalotiopsis theae), cucumber fusarium axysporum (Fusariumoxysporum f.sp.cucumerinum), cucumber anthracnose (Colletotrichum lagenarium), botrytis cinerea pers (Botrytis cinerea), Chinese cabbage alternaria (Alternaria brassicae), early blight of eggplant bacterium (Phytophthoraparasitica), pepper anthracnose bacterium (Colletotrichum capsici), Phytophthora capsici germ (Phytophora capsici), botrytis cinerea (Botrytis cirerea), cladosporium leaf and fruit mould of tomato bacterium (Fulvia fulva), tomato early blight bacterium (Alternariasolani), purple blotch of onion bacterium (Alternaria porri), green onion leaf spoting bacteria (Stemphylium botryosum), citrus scab bacterium (Sphaceloma fawcettii), citrus pathogens penicillium (Penicillium italicum), ring rot of apple bacterium (Botryosphaeria berengeriana f.sp.piricola), dry rot of apple bacterium (Botryosphaeria dothidea), alternaria leaf spot of apple bacterium (Alternaria mali Roberts), pear cucumerinum (Venturia nashicola), black rot of pear bacterium (Physalospora piricola).

1.2.2 live body is tested with bacterial classification and pot plant:

Puccinia striiformis (Puccinia striiformis f.sp.tritic), wheat leaf rust bacterium (Puccinia recondita f.sp.tritici), stem rust of wheat bacterium (Puccinia graminis Pers f.sp.tritici), wheat powdery mildew (Blumeriagraminis f.sp.tritici) and wheat (river wheat 107).

2 experimental techniques:

2.1 isolated experiment method:

2.1.1 neomycin is measured the antimicrobial spectrum of plant pathogenetic bacteria

Minimum inhibitory concentration (minimal inhibitory concentration, MIC) measure:

Respectively 1.1 described plant pathogenetic bacterias are diluted to finite concentration, join (ultimate density of neomycin adopts the test tube doubling dilution to make a series of neomycin concentration gradient medium) in the neomycin liquid nutrient medium, each series inoculation is a kind of for examination bacterium suspension, each concentration is done 3 repetitions, other gets 1 dilution series, does not inoculate any bacterium and does blank.After cultivating 18-24h under 35-37 ℃, will inoculate series and blank serial colorimetric estimation on 722 type spectrophotometers, the least concentration that the two OD value is identical is the minimum inhibitory concentration MIC of neomycin.

Minimum bactericidal concentration (minimal bactericidal concentration, MBC) measure:

Each pipe that the concentration of neomycin is higher than MIC (comprising MIC) continues to cultivate 18-24h, observes growing state.With the inoculation series and the colorimetric estimation on 722 spectrophotometers of blank series of extract, not having the least concentration of bacteria growing in the identical i.e. cultivation of the two OD value is minimum bactericidal concentration MBC.

Adopt growth rate method to measure bacterium colony inhibiting rate (%):

Respectively 1.1 described plant pathogenetic bacterias are diluted to finite concentration, join (ultimate density of neomycin adopts the test tube doubling dilution to make a series of neomycin concentration gradient medium) in the neomycin liquid nutrient medium, each series inoculation is a kind of for examination bacterium suspension, each concentration is done 3 repetitions, and makes blank with sterile water.After cultivating 18-24h under 35-37 ℃, these a series of cultures are carried out the bacterium numeration with the gradient dilution method, ask the bacterium colony inhibiting rate according to clump count, formula is as follows:

EC ₅₀And EC ₉₀Mensuration:

Logarithm with neomycin concentration is an ordinate, and neomycin is an abscissa to the probability value of the bacterium colony inhibiting rate correspondence of colony growth, sets up its inhibiting value probit value and concentration logarithm regression equation (virulence regression equation), and calculates EC ₅₀(inhibiting rate is 50% neomycin concentration) and EC ₉₀(inhibiting rate is 90% neomycin concentration).

2.1.2 neomycin is measured the antimicrobial spectrum of plant pathogenic fungi

Minimum inhibitory concentration (MIC) is measured:

Respectively the described plant pathogenic fungi of 1.2.1 is diluted to finite concentration, join (the ultimate density employing test tube doubling dilution of neomycin in the neomycin liquid nutrient medium, make a series of neomycin concentration gradient medium), each series inoculation is a certain amount of for examination bacterium suspension, each concentration is done 3 repetitions, other gets 1 dilution series, do not inoculate any bacterium and do blank, after cultivating 3d-5d under 27-28 ℃, with the inoculation series and the colorimetric estimation on 722 type spectrophotometers of blank series of extract, the least concentration that the two OD value is identical is minimum inhibitory concentration MIC.

(minimal fungicidal concentration MFC) measures: each pipe or the culture dish continuation cultivation 3d-5d that the concentration of neomycin are higher than MIC (comprising MIC) minimum antifungal concentration.With the inoculation series and the colorimetric estimation on 722 spectrophotometers of blank series of extract, not having the least concentration of bacteria growing in the identical i.e. cultivation of the two OD value is the minimum antifungal concentration MFC of extract.

Toxic medium therapy is measured mycelial growth inhibition rate (%):

With neomycin with after the sterile water dilution again with PDA medium mixing, the ultimate density of neomycin adopts doubling dilution, makes the uniform a series of pastille flat boards of a series of thickness, as blank, every processing repeats 3 times with sterile water.Treat to supply examination bacterium cake with connecting plant pathogenic fungi after the culture medium solidifying, bacterium cake diameter is 0.6cm, and mycelia faces down, and the right-angled intersection method is measured colony diameter behind the 27-28 ℃ of cultivation 3d-5d, calculates mycelial growth inhibition rate.

EC ₅₀And EC ₉₀Mensuration:

Logarithm with neomycin concentration is an ordinate, and neomycin is an abscissa to the probability value of the inhibition growth percentage correspondence of colony growth, sets up its inhibiting value probit value and concentration logarithm regression equation (virulence regression equation), and calculates EC ₅₀(inhibiting rate is 50% neomycin concentration) and EC ₉₀(inhibiting rate is 90% neomycin concentration).

2.2 experiment made on the living method:

Adopt the greenhouse pot culture method.Puccinia striiformis, wheat leaf rust bacterium, stem rust of wheat bacterium, wheat powdery mildew are all broadcast back 14-16d at the dressing wheat and are divided the winding bacterium respectively.Each is handled and repeats every repetition 3 basins, every basin 10-20 strain 3 times.After connecing bacterium, wheat is placed 13-17 ℃ of greenhouse, check incidence about 20d.The plant of attention observation simultaneously emerges and grows situation.In addition, 2000 times of dilutions of the triadimefon WP with 15% and running water in contrast, yellow rust, leaf rust, black stem rust, powdery mildew investigation are all undertaken by its grade scale, calculate disease index and relative control effect.

3 experimental results

Neomycin sees Table 1 to the antimicrobial spectrum measurement result of 1.1 described plant pathogenetic bacterias.By table 1 result as can be known, neomycin all has very significantly antibacterial and bactericidal action to plant pathogenetic bacteria, when neomycin concentration reaches 16mg/L, all plant pathogenetic bacterias all had killing action, and neomycin also all is not more than 8mg/L to the minimum inhibitory concentration of all plant pathogenetic bacterias, in addition, further studied EC50, the EC90 of neomycin to plant pathogenetic bacteria.Table 1,2 result have shown that clearly neomycin is to being the fairly obvious broad spectrum of bacteria bactericide of a kind of effect.

Table 1 neomycin is to the MIC and the MBC (mg/L) of plant pathogenetic bacteria

Bacterial classification	MIC	MBC	Bacterial classification	MIC	MBC
						Xanthomonas oryzae	2	4	Paddy rice basal stem rot bacterium	0.5	2
Rice bacterial stripe bacterium	1	2	The cotton angular leaf spot fungus	4	16
						The corn bacterial wilt	2	4	Tobacco ralstonia solanacearum	2	16
The bacillary prairie fire germ of tobacco	1	4	Tobacco hollw stalk bacterium	1	2
						Tobacco bacterial leaf spot bacterium	2	8	The bacterial blight of soybean bacterium	1	8
Soybean macula germ	4	8	The bacterial wilt of peanut bacterium	4	8
						The potato bacterial wilt bacterium	2	4	The potato rot positive germ	2	16
The potato bacterial ring rot bacterium	1	8	The soft rot of cabbage bacterium	1	1
						The Chinese cabbage black rot	1	16	The bacterial wilt of tomato bacterium	0.5	1
Bacterial canker of tomato	2	16	Ginger ginger plague germ	2	8
						The cucumber bacterial angular leaf spot bacterium	2	8	The konjak soft rot bacterium	1	4
The cabbage black rot bacterium	4	8	The Chinese cabbage alternaria	0.5	2
						Pepper scab fungus	0.5	2	Candidatus liberobacter asiaticum	2	4
The c itrus canker germ	0.5	2	Apple crown gall germ	1	4

Table 2 neomycin is to the EC of plant pathogenetic bacteria ₅₀, EC ₉₀Value (mg/L)

Bacterial classification	EC50	EC90	Bacterial classification	EC50	EC90
						Xanthomonas oryzae	0.224	1.104	Paddy rice basal stem rot bacterium	0.056	0.269
Rice bacterial stripe bacterium	0.145	0.572	The cotton angular leaf spot fungus	0.450	1.981
						The corn bacterial wilt	0.385	1.021	Tobacco ralstonia solanacearum	0.224	0.879
The bacillary prairie fire germ of tobacco	0.216	0.857	Tobacco hollw stalk bacterium	0.223	0.856
						Tobacco bacterial leaf spot bacterium	0.164	0.109	The bacterial blight of soybean bacterium	0.151	0.558
Soybean macula germ	0.522	2.102	The bacterial wilt of peanut bacterium	0.323	1.954
						The potato bacterial wilt bacterium	0.220	1.075	The potato rot positive germ	0.257	1.092
The potato bacterial ring rot bacterium	0.097	0.554	The soft rot of cabbage bacterium	0.101	0.585
						The Chinese cabbage black rot	0.065	0.515	The bacterial wilt of tomato bacterium	0.074	0.255
Bacterial canker of tomato	0.232	1.057	Ginger ginger plague germ	0.251	0.987
						The cucumber bacterial angular leaf spot bacterium	0.201	1.065	The konjak soft rot bacterium	0.113	0.552
The cabbage black rot bacterium	0.354	2.065	The Chinese cabbage alternaria	0.068	0.234
						Pepper scab fungus	0.052	0.225	Candidatus liberobacter asiaticum	0.256	1.019
The c itrus canker germ	0.035	0.238	Apple crown gall germ	0.114	0.525

Neomycin or its salt are as the sterilization pesticide of plant pathogenetic bacteria, and the concentration range that neomycin or its salt use is can be 5-500mg/L.During the preparation agricultural chemicals, the active constituent content in the preparation is 5-50%, is diluted to the use of 5-500mg/L concentration range during use and gets final product.

Below be the antimicrobial spectrum measurement result of neomycin, and neomycin is seen by table 3, table 4 EC50, the EC90 of these plant pathogenic fungis to plant pathogenic fungi (the live body test is except bacterial classification).As can be seen from Table 3, neomycin also has suitable bacteriostasis to plant pathogenic fungi, when neomycin concentration reaches 512mg/L, all plant pathogenic fungis are all had killing action, and minimum inhibitory concentration also all is not more than 256mg/L.This shows that neomycin can also be a kind of broad spectrum fungus bactericide.

Table 3 neomycin is to MIC and the MFC (mg/L) of plant pathogenic fungi

Bacterial classification	MIC	MFC	Bacterial classification	MIC	MFC
						Pyricularia oryzae	64	128	Fusarium moniliforme	128	256
Rhizoctonia solani Kuhn	128	128	Fusarium graminearum	128	256
						The root rotof flax bacterium	64	128	Rhizoctonia cerealis	256	512
Gaeumannomyces graminis	64	256	Exserohilum turcicum	128	128
						Corn is bent the spore leaf spot fungi	256	512	Southern corn leaf blight	32	64
The cotton anthracnose bacterium	64	128	Cotton rhizoctonia solani	64	128
						Cotton-wilt fusarium	128	256	Verticillium dahliae	64	128
Sclerotinia sclerotiorum	256	512	Peanut Cercospora bacteria	64	128
						Cercospora leaf spot of groundnut bacterium	128	256	The sweet potato pine root fungus	128	256
Phytophthora infestans	64	128	The soybean anthracnose bacterium	64	128
						The anthracnose of tobacco bacterium	64	128	Tobacco black shank bacterium	64	128
Tobacco brown spot pathogen	256	512	Tea zonate spot bacterium	256	512
						Cucumber fusarium axysporum	64	256	Cucumber anthracnose	128	512
Botrytis cinerea pers	256	512	The Chinese cabbage alternaria	64	256
						The early blight of eggplant bacterium	64	128	The pepper anthracnose bacterium	256	512
The Phytophthora capsici germ	256	512	Botrytis cinerea	64	128
						The cladosporium leaf and fruit mould of tomato bacterium	64	128	Tomato early blight bacterium	128	512
The purple blotch of onion bacterium	128	256	The green onion leaf spoting bacteria	32	64
						The citrus scab bacterium	128	256	Citrus pathogens penicillium	256	512
The ring rot of apple bacterium	64	128	The dry rot of apple bacterium	128	256
						The alternaria leaf spot of apple bacterium	64	256	Pear cucumerinum	128	512
The black rot of pear bacterium	128	256

Table 4 neomycin is to the EC of plant pathogenic fungi ₅₀, EC ₉₀Value (mg/L)

Bacterial classification	EC50	EC90	Bacterial classification	EC50	EC90
						Pyricularia oryzae	9.65	32.51	Fusarium moniliforme	12.14	60.31
Rhizoctonia solani Kuhn	10.65	58.34	Fusarium graminearum	15.12	65.37
						The root rotof flax bacterium	7.26	32.25	Rhizoctonia cerealis	20.89	124.51
Gaeumannomyces graminis	7.69	35.79	Exserohilum turcicum	10.68	59.99
						Corn is bent the spore leaf spot fungi	20.26	120.21	Southern corn leaf blight	3.04	15.52
The cotton anthracnose bacterium	6.98	36.57	Cotton rhizoctonia solani	5.46	30.79
						Cotton-wilt fusarium	11.29	61.79	Verticillium dahliae	9.87	31.64
Sclerotinia sclerotiorum	30.18	124.82	Peanut Cercospora bacteria	9.14	31.79
						Cercospora leaf spot of groundnut bacterium	16.24	65.81	The sweet potato pine root fungus	14.71	70.12
Phytophthora infestans	10.25	40.21	The soybean anthracnose bacterium	9.82	38.16
						The anthracnose of tobacco bacterium	9.84	34.47	Tobacco black shank bacterium	8.35	30.79
Tobacco brown spot pathogen	40.21	135.82	Tea zonate spot bacterium	44.35	130.38
						Cucumber fusarium axysporum	9.24	38.56	Cucumber anthracnose	16.24	69.61
Botrytis cinerea pers	45.61	121.77	The Chinese cabbage alternaria	9.41	33.68
						The early blight of eggplant bacterium	7.57	37.82	The pepper anthracnose bacterium	36.17	137.16
The Phytophthora capsici germ	20.78	126.57	Botrytis cinerea	7.98	38.01
						The cladosporium leaf and fruit mould of tomato bacterium	9.78	34.21	Tomato early blight bacterium	15.24	62.12
The purple blotch of onion bacterium	13.98	67.19	The green onion leaf spoting bacteria	4.67	16.85
						The citrus scab bacterium	21.25	70.25	Citrus pathogens penicillium	30.87	115.31
The ring rot of apple bacterium	10.54	35.86	The dry rot of apple bacterium	15.51	69.24
						The alternaria leaf spot of apple bacterium	9.54	34.72	Pear cucumerinum	24.28	75.87
The black rot of pear bacterium	16.86	69.97

Result to the potted plant effect experiment (table 5,6,7,8) that can only carry out plant pathogenic fungi puccinia striiformis that live body cultivates, wheat leaf rust bacterium, stem rust of wheat bacterium, wheat powdery mildew shows; neomycin has demonstrated obvious protective effect; when concentration is 64mg/L; its drug effect reaches 65.57%, 62.81%, 66.52% and 63.68% respectively; also possesses simultaneously therapeutic action preferably; when concentration was 64mg/L, its drug effect reached 69.50%, 72.38%, 60.78% and 62.77% respectively.

Table 5 neomycin is to the preventive effect of wheat stripe rust

Handle	Concentration (mg/L)	Protective effect		Therapeutic action
						Disease index	Preventive effect (%)	Disease index	Preventive effect (%)
		Neomycin	64	27.72	65.57					25.49	69.50
Triadimefon	7.5					14.82	81.59	10.03	88.00
		Running water	-	80.51	-					83.56	-

Table 6 neomycin is to the preventive effect of puccinia triticinia

Handle	Concentration (mg/L)	Protective effect		Therapeutic action
						Disease index	Preventive effect (%)	Disease index	Preventive effect (%)
		Neomycin	64	30.81	62.81					21.85	72.38
Triadimefon	7.5					16.79	79.58	11.49	85.48
		Running water	-	82.84	-					79.12	-

Table 7 neomycin is to the preventive effect of stem rust of wheat bacterium

Handle	Concentration (mg/L)	Protective effect		Therapeutic action
						Disease index	Preventive effect (%)	Disease index	Preventive effect (%)
		Neomycin	64	26.48	66.52					29.13	60.78
Triadimefon	7.5					14.22	82.04	11.85	84.16
		Running water	-	79.09	-					74.28	-

Table 8 neomycin is to the preventive effect of wheat powdery mildew

Handle	Concentration (mg/L)	Protective effect		Therapeutic action
						Disease index	Preventive effect (%)	Disease index	Preventive effect (%)
		Neomycin	64	28.15	63.68					25.89	62.77
Triadimefon	7.5					14.27	81.59	8.29	88.08
		Running water	-	77.51	-					69.54	-

Neomycin or its salt are as the sterilization pesticide of plant pathogenic fungi, and the concentration range that neomycin or its salt use is can be 20-2000mg/L.During the preparation agricultural chemicals, the active constituent content in the preparation is 5-50%, is diluted to the use of 20-2000mg/L concentration range during use and gets final product.

Test example 2

1 for the examination pest

Cabbage caterpillar (Pieris rapave L.), cotten aphid (Aphis gossypii Glover), wide must adopting to Sichuan University agricultural chemicals laboratory experiment field by ant locust [MyrmeleotettixPolpalis (Zub)], therefrom select the 3-4 instar larvae of polypide uniformity, hungry 5-6h before the test.

2 test methods

2.1 neomycin is to the antifeedant activity of pest

Adopt the blade infusion process, fresh cabbage leaves and maize leaf are broken into the leaf dish with the card punch of diameter 2cm, with treatment fluid with contrast liquid and press gradient dilution.The leaf dish of handling for the examination worm feeding of processed group with neomycin, the leaf dish that the examination worm feeding of control group was handled with clear water, wherein cabbage caterpillar and aphid feeding cabbage leaves dish, locust feeding maize leaves dish.The examination worm all selected for 3 ages for use, and every group 20 cephalont repeated 3 times.Put room temperature (25 ℃), handle 24h, 48h and 72h respectively and observe, the food area got in the graph paper record, calculates the food refusal rate.

2.2 neomycin is to the contact toxicity of pest

Adopt dip method, the examination worm is dipped in the neomycin aqueous solution of handling by gradient and contrast clear water solution, takes out behind the 3s, treat to be positioned over dependent insect cage after polypide surface soup dries, feeding is with fresh cabbage leaves (cabbage caterpillar, aphid) or fresh maize leaf (locust).Select the 3-4 examination worm in age for use, every group 20 cephalont repeated 3 times.Put room temperature (25 ℃), handle 24h, 48h and 72h, record examination worm death condition, calculation correction lethality.

LC ₅₀Mensuration:

Logarithm with neomycin concentration is an ordinate, and neomycin is an abscissa to the probability value of the corrected mortality correspondence of pest, sets up its inhibiting value probit value and concentration logarithm regression equation (virulence regression equation), and calculates LC ₅₀(corrected mortality is 50% neomycin concentration).

3 experimental results

The active result of 24h food refusal rate of cabbage caterpillar, cotten aphid, locust and 72h sees Table 9 respectively.As can be seen, neomycin is the highest to the contact toxicity of cabbage caterpillar, the lethal concentration of 50 (LC of 72h ₅₀) be 367.37mg/L, secondly be locust, relatively low to the contact toxicity of cotten aphid, be 579.70mg/L.Therefore, as can be seen, neomycin has certain preventive and therapeutic effect to pest cabbage caterpillar, cotten aphid, locust.

Table 9 neomycin is to the tagging of pest, antifeedant activity

The examination worm	Concentration (mg/L)	Corrected mortality (100%) (72h)	LC 50 (mg/L)	Food refusal rate (100%) (24h)
					Cabbage caterpillar	3200 1600 800 400 200	93.82 87.15 83.47 61.68 24.05	367.37	94.76 88.23 69.50 54.16 28.34
Cotten aphid	6400 3200 1600 800 400	93.15 86.71 79.15 58.12 28.75	579.70	96.57 88.46 70.32 52.78 30.47
					Locust	3200 1600 800 400 200	92.15 85.42 75.49 52.17 30.02	376.61	98.57 90.85 71.46 56.21 32.14

According to above-mentioned result of the test, neomycin or its salt are as the agricultural chemicals at pest, and the concentration range that neomycin or its salt use is can be 200-4000mg/L.During the preparation agricultural chemicals, the active constituent content in the preparation is 10-50%, is diluted to the use of 200-4000mg/L concentration range during use and gets final product.

In sum, the neomycin of the present invention narration be a kind of wide spectrum phytopathogen is had the antibiotic of better activity, particularly the effect at plant pathogenetic bacteria is very outstanding especially, in addition, pest cabbage caterpillar, aphid and locust is also had certain preventive and therapeutic effect.Its exploitation is become novel agrochemical, have stable, efficient, nontoxic feature,, have bright prospects at pesticide field.

Claims (7)

1, neomycin or the purposes of its salt in agricultural chemicals.

2, purposes according to claim 1 is characterized in that: described salt is the sulphate of neomycin.

3, purposes according to claim 1 is characterized in that: the purposes of the described purposes plant disease that to be neomycin or its salt cause in control bacterium, fungi or virus.

4, purposes according to claim 3, it is characterized in that: described plant disease is that bacterium, fungi or virus cause paddy rice, wheat, corn, tobacco, peanut, potato, Chinese cabbage, tomato, eggplant, the disease of cucumber, green onion, ginger, konjaku, wild cabbage, capsicum, citrus, pears or apple.

5, purposes according to claim 1 is characterized in that: the purposes of the described purposes plant disease that to be neomycin or its salt cause pest.

6, purposes according to claim 5 is characterized in that: described pest are cabbage caterpillar, cotten aphid or locust.

7, agricultural chemicals is characterized in that: with neomycin or its salt is pesticide activity component, and the acceptable auxiliary element is prepared from the adding agricultural chemicals.