TWI731069B - Microbial pesticide formulation composition, and method of production and use thereof - Google Patents

Abstract

Provided herein is a microbial pesticide formulation composition that, by containing acetic acid, improves the activity of the biopesticidal filamentous fungus contained as an active pesticide component having a disease and/or pest and/or weed control effect, and, a plant growth regulating effect while being preservable for long periods of time. A method for producing such compositions, and a method for controlling pests and weeds, and a method for regulating plant growth with the use of the composition are also provided, among others. This is achieved with use of a pesticide formulation composition that includes: a biopesticidal filamentous fungus solid culture using a solid medium which is at least one selected from a grain seed, and a hull, bran, and a polished product of a grain seed; and acetic acid.

Description

Microbial pesticide formulation composition, its manufacturing method and use method

The present invention relates to microbial pesticide preparation compositions and the like. In detail, it relates to the use of filamentous fungi exhibiting control effects on diseases, pests, weeds, etc., plant growth adjustment effects, etc., as active ingredients for pesticides, the use of acetic acid to enhance the control activity of the filamentous fungi, etc., and directly use The long-term survival of the filamentous bacteria is made possible by the activated state of acetic acid, that is, the highly activated microbial pesticide formulation composition with excellent storage stability, its manufacturing method, and the pests, weeds, etc. used by the use Control methods, plant growth adjustment methods, etc.

The control of useful plant diseases and insect pests or the control of weeds efficiently performs operations that are indispensable to agricultural production. For this purpose, synthetic pesticides have been used, which is a major achievement. However, in recent years, it has become possible to cite the problem of the production of resistant pests or environmental damage due to the large number of synthetic pesticides. How to reduce the environmental load efficiently and whether it can be sustained? Sexual agricultural production has become an important issue in the agricultural field.

As one of its solutions, microbial pesticides that utilize microbial functions have been proposed, and it is recognized that by using them alone or in combination with them to synthesize pesticides, they have the effect of reducing environmental load, or synthetic pesticides have become a major problem for pesticide-resistant pests. Or the effect of suppressing the frequency of occurrence of drug-resistant weeds.

Among the useful microorganisms that show the control activity against pests, diseases, weeds, etc., and improve agricultural productivity, there are fungi composed of tubular cells called hyphae, which are collectively referred to as biopesticide filamentous bacteria. A wide range of reports have been made regarding the technology of using this biopesticide filamentous fungus as agricultural materials. For example, Patent Document 1 discloses a disease control agent using Talaromyces as an active ingredient in agrochemicals, and Patent Document 2 discloses There are pest control agents or disease control agents that use Beauveria, Black bass, Paecilomyces, Aspergillus, Penicillium, and Trichoderma as active ingredients of pesticides. Patent Document 3 A disease control agent using Verticillium as a pesticide active ingredient is disclosed, and Patent Document 4 discloses a weed control agent using Drechslera as a pesticide active ingredient.

On the other hand, it is considered that the biopesticide filamentous bacteria are applied simultaneously with acetic acid to improve the control activity, etc. For example, Patent Document 5 discloses that the combined use of Trichoderma and acetic acid improves the Zoysia of the Trichoderma spp. An example of the control activity of grass leaf rot.

In this way, the beneficial effects of the combined use of biopesticide filamentous bacteria and acetic acid, although some are known, but mixed biopesticide filamentous bacteria and acetic acid The insights of the composition of the composition are still in the use scene. Those who are randomly mixed and prepared on-site mixtures. From the mixing and preparation at the manufacturing stage to the use through circulation, the report of the single-package pesticide formulations is based on the prerequisite for the preservation period Did not find out. Still, in the present invention, in order to distinguish between the on-site mixture that is used immediately after preparation, that is, the pesticide composition, and the single-packaged pesticide formulation that is the industrial product that is intended to be preserved, the latter refers only to the latter called "pesticide composition. ".

The reason for staying in such an on-site mixture is already clear. This is because it has a bactericidal effect on biological pesticide filamentous bacteria whose acetic acid is the active ingredient of the pesticide. As an attempt, when the bacteria of Trichoderma asperelloides SKT-1 were added to an ^{aqueous suspension containing 1×10 11} CFU (Colony Forming Unit)/L with acetic acid, the bacteria of SKT-1 were completely eliminated within 24 hours after the addition. . A simple mixture of biopesticide filamentous bacteria and acetic acid, although it improves the control activity in a very short period after mixing, it is impossible to store because the bacteria die out over time. As an industrial product, it is used as a pesticide formulation composition system that requires preservation stability Those who cannot be established.

Among such prior technologies, in the industry, there is a strong expectation for the development of a formulation technology that allows the biopesticide filamentous bacteria to remain indifferent over time (which can be stored for a long time) even if the biopesticide filamentous bacteria are used in combination with acetic acid.

[Prior Technical Literature] [Patent Literature]

[Patent Document 1] JP 2007-31294 A

[Patent Document 2] Japanese Patent Application Publication No. 7-48216

[Patent Document 3] JP 2006-169115 A

[Patent Document 4] Japanese Patent Laid-Open No. 6-277042

[Patent Document 5] Japanese Patent Application Laid-Open No. 9-87122

The present invention provides a biopesticide filamentous fungus which is a pesticide active ingredient, which is highly activated by acetic acid and can be stored for a long time, a method for manufacturing the same, and a method for controlling pests or weeds by the use , Plant growth adjustment methods, etc. as the purpose.

In order to achieve the above-mentioned object, the inventors of the present inventors have studied hard and found that it contains a biopesticide filamentous fungus solid that uses grain seeds and/or seed processed products (hull, bran, and white matter any one or more) as a solid medium. The microbial pesticide formulation composition composed of culture and acetic acid can solve the above-mentioned problems, and finally the present invention is completed.

That is, the embodiment of the present invention is as follows.

(1) A pesticide preparation composition containing a solid culture of biological pesticide filamentous fungus and acetic acid. The solid culture of biological pesticide filamentous fungus is selected from grain seeds, outer skins, bran, and whites. One or more of them are used as a solid medium.

(2) The pesticide formulation composition according to (1), wherein the biological pesticide filamentous fungus is a filamentous fungus that has the effect of preventing pests and/or weeds and adjusting the growth of plants.

(3) The pesticide formulation composition according to (2), wherein the filamentous fungus that has the effect of controlling pests and/or weeds and adjusting the growth of plants belongs to the genus Beauveria, Scutellaria Coniothyrium, Metarhizium, Talaromyces, Trichoderma, Verticillium.

(4) The pesticide formulation composition according to (3), wherein the filamentous fungus having the effect of controlling pests and/or weeds and adjusting the growth of plants is Trichoderma.

(5) The pesticide formulation composition according to (4), wherein the Trichoderma is asperelloides, asperellum, atroviride, hamatum, Any of the species of Trichoderma harzianum and Trichoderma koningii (koningii).

(6) The pesticide formulation composition according to (5), wherein the Trichoderma is a species of asperelloides.

(7) The pesticide formulation composition according to (6), wherein the asperelloides species is Trichoderma asperelloides SKT-1 strain (FERM BP-16510).

(8) The pesticide formulation composition of any one of (1) to (7), which is contained in 1 g of the pesticide formulation composition, containing ^{10-10 10} CFU (Colony Forming Unit) of biological pesticide filamentous bacteria.

(9) The pesticide formulation composition according to any one of (1) to (8), wherein the solid medium is the white substance of grain seeds.

(10) The pesticide formulation composition according to any one of (1) to (9), wherein the grain is a gramineous grain.

(11) The pesticide formulation composition according to (10), wherein the gramineous grain is barley.

(12) The pesticide formulation composition according to any one of (1) to (11), wherein the content of acetic acid in the pesticide formulation composition is 0.01 to 1% by mass, for example, 0.04 to 0.6% by mass.

(13) The pesticide preparation composition of any one of (1) to (12), which is a granular pesticide preparation composition, and the solid medium is a granular substance.

(14) The pesticide formulation composition according to (13), wherein the acetic acid is an acetic acid adsorbent formed by adsorbing acetic acid on a fine mineral powder.

(15) The pesticide formulation composition according to (14), wherein the mineral micropowder is aqueous amorphous silica.

(16) The pesticide formulation composition of (14) or (15), wherein the content of the acetic acid adsorbent is 0.5-2% by mass in the pesticide formulation composition.

(17) A method for manufacturing a pesticide formulation composition, which is characterized in that the biopesticide filamentous fungus uses one or more selected from grain seeds, outer skins, bran, and whites as a solid medium for solid culture to obtain a cultured filamentous fungus. After the biological pesticide filamentous bacteria are attached to the solid culture of the biological pesticide filamentous bacteria on the solid medium, acetic acid is added to the culture.

(18) The method according to (17), wherein the pesticide formulation composition is granular, and the solid culture medium is granular.

(19) The method of (17) or (18), which is used on the surface of the solid culture of filamentous biopesticide, by spraying acetic acid or acetic acid with water or A solution diluted with organic solvents, while supporting acetic acid.

(20) The method according to (18), which involves adsorbing acetic acid on the fine mineral powder, and coating the adsorbent on the surface of the solid culture of biopesticide filamentous bacteria.

(21) The method according to (20), wherein the mineral powder is hydrous amorphous silica.

(22) A method for controlling and/or adjusting plant growth of one or more species selected from the group consisting of weeds, diseases, and pests, characterized in that the pesticide formulation composition of any one of (1) to (16) is applied to Plant seedlings, seedlings or seeds, and cultivate the plant.

(23) A method for controlling and/or adjusting plant growth of one or more species selected from the group consisting of weeds, diseases, and pests, characterized in that the pesticide formulation composition of any one of (1) to (16) is applied In the soil where plants are cultivated.

(24) The method of (23), which is to apply 5~1000g, for example 100~1000g, of the pesticide formulation composition ^{per 1 m 2 of the soil.}

According to the present invention, it is possible to provide a pesticide composition (microbial pesticide formulation composition) which is a biological pesticide filamentous fungus which is a pesticide active ingredient and is highly activated by acetic acid and can be stored for a long time in a formulation form that does not require immediate preparation on site. The manufacturing method, the control method of pests, weeds, etc., the method of plant growth adjustment etc. by the use.

The microbial pesticide formulation composition of the present invention is characterized by Contains the biopesticide filamentous bacteria useful in agriculture as a solid culture (using biopesticide filamentous bacteria solid culture, that is, using the cultured biopesticide filamentous fungus cells (including hyphae or spores) and grains The mixture of the solid medium of the seed and/or seed processing), and acetic acid.

The term "biological pesticide filamentous fungus" in the present invention means to improve the soil by preventing pests, pests, weeds and other harmful organisms, making the soil suitable for farming, etc., promoting or inhibiting the growth of plants themselves, etc. The filamentous bacteria of agricultural production are not limited to those registered as pesticides or those whose effects are expressly used as pesticides. Examples thereof include bacteria of the genus Acremonium, bacteria of the genus Alternaria, bacteria of the genus Ambrosiella, bacteria of the genus Arthrobotrys, bacteria of the genus Aspergillus, and Basidiomycetes aureus (Aureobasidium), Beauveria (Beauveria), Blastomyces, Botriosporium, Botrytis, Chalara, Cercospora ), Cephalosporium, Chrysomnia, Chrysosporium, Chlorodoim, Cladosporium, Coccidioides, Shield Coniothyrium, Cryptococcus, Cunninghamella, Curvularia, Cylindrocarpon, Cylindrocarpon, Cylindrocarpon (Cylindrocladium), Drechslera, Epicoccum, Penicillium (Eupenicillium), Fusarium, Geotrichum, Gliocladium, Graphium, Helicomyces Bacteria, Helicosporium (Helicosporium), Heliscus, Helmithosporium, Hyalodendron, Hystoplasma, Isaria, Lemonniera Bacteria, Metarhizium, Microsporium, Monilia, Monosporium, Mortierella, Mortierella, Mucor, Nigrospora, Nodulisporium, Nomuraea, Oidiodendron, Oidium Genus, Paecilomyces, Penicillium, Phialomyces, Phialophora, Pyricularia, Raffaelea, Ramularia Genus, Rhinocladiella, Rhizoctonia, Sporothrix, Staphylotrichum, Stilbella, Talaromyces Genus, Thermomyces, Trichophyton, Trichoderma, Trichothecium, Tripospermum, Varicose Varicosporium, Verticillium, etc. If it has the control activity against pests Etc., well-known biological pesticide filamentous bacteria can be used arbitrarily. Although the present invention is not limited to the above-exemplified bacteria, among them, it is preferable to use Beauveria bassiana, Scutellaria spp., and Nigrosporia Bacteria of the genus, Talaromyces, Trichoderma, and Verticillium, particularly preferably Trichoderma. Among the Trichoderma species, asperelloides species, asperellum species, atroviride species, hamatum species, harzianum species, corning wood As a suitable example, koningii inoculum, etc., are particularly preferred as asperelloides inoculum. Among asperelloides inoculum, they can be found in the Licensed Microorganism Trust Center of the Institute of Biotechnology and Industrial Technology, Institute of Industry and Technology, Ministry of International Trade and Industry (now an independent administrative legal entity, product evaluation technology base organization, licensed organism Trust center), after the SKT-1 strain of dark green wood (Trichoderma atroviride) with the previous name as the trust on November 10, 1997 (Heisei 9), it was transferred to the international market on February 13, 2017 (Heisei 29). The trusted Trichoderma asperelloides SKT-1 strain (FERM BP-16510) is particularly good.

Although the number of filamentous bacteria (the number of bacteria and/or spores) in the pesticide formulation composition of the present invention is not specified, the pesticide formulation composition is usually 10~10 ¹⁰ CFU (Colony Forming Unit: Colony Forming Unit) per 1g. ), preferably about 10 ³ to 10 ⁹ CFU, more preferably about 10 ⁵ to 10 ⁸ CFU. The number of filamentous bacteria in the pesticide formulation composition can be determined by the dilution plate method. As the medium used in the dilution plate method, for example, ordinary agar culture medium, standard agar culture medium, potato dextrose agar culture medium, oatmeal agar culture medium, malt extract agar culture medium, potato carrot agar culture medium, sea water Starch agar culture medium, Czapek'dox agar culture medium, Czepec yeast extract agar culture medium, MY20 agar culture medium, Sabrow. Glucose agar culture medium, Lulia Bertani agar culture medium and other well-known culture media, but not limited to these examples, it is only necessary to use a culture medium consistent with the nutrient requirements of the obtained treatment biopesticide filamentous fungus. In addition, in the dilution plate method, for the purpose of optimizing the medium to be measured, the addition or removal of medium components, the addition and subtraction of the amount, etc., are often carried out, but they can be arbitrarily performed based on the knowledge of a person with ordinary knowledge in the field of the present invention. get on. In addition, the conditions such as the culture temperature or the culture period can also be arbitrarily set according to the optimal temperature or colony formation speed for processing biological pesticide filamentous bacteria.

In addition, in the pesticide formulation composition of the present invention, the solid medium for the solid culture of the biopesticide filamentous fungus uses one or more selected from the group consisting of seeds of cereals, their husks, bran, and whites. In the present invention, the term "grain" is used in a broad sense. As examples, in addition to rice, corn (maize), barley, wheat, rye, oat ), wild oat, adlay, proso millet, foxtail millet, millet, sorghum, finger millet, pearl millet, In addition to gramineous grains such as teff, fonio, kodo millet, and manchurian wild rice, soybean, azuki bean, etc. Mung bean, cowpea, common bean, emperor Lima bean, peanut, pea, broad bean, lentil, chickpea, runner bean, black gram, moth bean moth bean, tepary bean, ricebean, hyacinth bean, horse gram, bambara groundnut, geocarpa groundnut, pigeon pea ), sword bean, jack bean, grass pea, cluster bean, winged bean, cowhage, long horn Leguminous grains such as carob, lupine, tamarind, etc., and further examples include Polygonaceae plants such as buckwheat and tartary buckwheat, Amaranthaceae plants, Chenopodiaceae plants, etc. It is suspected of cereals. Any edible seeds with starch as the main body can be used. Although the present invention is not limited to the above-mentioned exemplified grains, among them, gramineous or leguminous grains are preferred, among which gramineous or leguminous grains are particularly preferred. It is 1 or more of any one of barley, wheat, rice, and soybean. Use more than one species selected from grain seeds, their hulls, bran, and whites in the solid medium, and by using the solid culture containing this in the formulation, the long-term biopesticide filamentous bacteria in the presence of acetic acid is improved Survivability.

Although the seeds of the aforementioned grains can be used directly (for example, they also include those that peel off the husks such as brown rice or brown wheat), but the husks (bran, etc.) themselves that appear when the seeds are peeled can also be used, and they appear when they are white. The bran, the white substance itself, the mixture of these 2 or more. Still, in the present invention, there are The case where the skin, bran, and white matter of seeds are collectively referred to as "processed seeds". In addition, although the white substance of the seed is substantially composed of the endosperm of the seed, and the endosperm associated with the germ as described above, both can be used in the present invention, and there is no particular difference in the present invention.

Such grain seeds or seed processed products can be directly used as solid medium users. In the present invention, the grain seeds and/or seed processed products are used in the solid medium to cultivate biological pesticide filamentous bacteria, and the resulting biological The solid culture of pesticide filamentous bacteria uses the raw material of the pesticide preparation composition in each medium.

The seeds of cereals and their white matter are originally granular, and the outer skin and bran of the seeds are originally indefinite. These granular or amorphous substances can also be crushed to become finer particles or powders (granular crushed product, powdered crushed product, etc.), but they can also be used directly without crushing. The original shape. The pesticide formulation composition of the present invention determines its dosage form by the shape of the grain seed and/or seed processed product used, although it can be a granular pesticide formulation composition, an indefinite shape pesticide formulation composition or a powdered pesticide formulation The composition is preferably a granular pesticide formulation composition, especially a granular material with a particle size of 2 mm or more. According to this, among the aforementioned grain seeds and/or seed processed products, it is preferable For those belonging to granular materials, among the granular materials, it is particularly preferable to use the refined white materials of grain seeds.

The method for culturing the filamentous bacteria of the biological pesticide of the present invention can be arbitrarily set according to the type and strain of the filamentous bacteria. In addition, as the solid medium (carrier of the solid medium) to be cultured, the seeds and/or seed processed products of the aforementioned cereals are used. For the purpose of increasing the efficiency of proliferation, you can add Add carbon source, nitrogen source, inorganic salt, etc. The culture temperature and culture time can also be set arbitrarily, but for example, the conditions for culturing at 10-40°C for 2-30 days are shown. In addition, the resulting culture can be appropriately dried.

Furthermore, the pesticide formulation composition of the present invention incorporates acetic acid for the purpose of activating the activity of biopesticide filamentous bacteria. By using acetic acid to promote the mycelial growth of biopesticide filamentous fungi and enhance the activity as pesticide active ingredients, the pesticide formulation composition of the present invention achieves a high control effect. Although the blending amount of the aforementioned acetic acid is not particularly limited, if the amount of acetic acid is too small, a sufficient mycelial growth promoting effect cannot be expected. On the other hand, the extremely excessive acetic acid can affect the biological pesticide filamentous bacteria in the pesticide formulation composition. The case where the bactericidal effect is performed. For biopesticide filamentous bacteria, a sufficient mycelial growth promotion effect is expected, and the blending amount of biopesticide filamentous bacteria with suitable acetic acid that does not sterilize is usually 0.01~1% by mass based on the total amount of the pesticide formulation composition. It is preferably 0.03 to 0.8% by mass, more preferably 0.05 to 0.5% by mass.

Although an example of a method of blending acetic acid into a pesticide formulation composition is shown below, the present invention is not limited to these methods. First, when the culture medium is a powder, for example, acetic acid is added to the solid culture of biopesticide filamentous bacteria and further mixed to homogenize the acetic acid in the powdery pesticide formulation composition. On the other hand, when the medium is granular, acetic acid may be supported on the surface. As a method of supporting acetic acid on the surface of the granular material, for example, although it can also be a method of spraying a solution of acetic acid or acid diluted with water or an organic solvent on the granular material, it is preferable to first apply an aqueous amorphous dioxide Silica (white carbon black) and other mineral powders adsorb acetic acid, and then, The method of covering the surface of the solid culture medium with the acetic acid adsorbent further improves the long-term viability of the biopesticide filamentous bacteria in the presence of acetic acid. In the case where acetic acid is supported on the surface of the granular material using fine mineral powder, the blending amount of the fine mineral powder supporting acetic acid is not particularly limited, but it is usually 0.2 to 3% by mass based on the total amount of the pesticide formulation composition. It is preferably 0.5 to 2% by mass, more preferably 0.8 to 1.5% by mass.

As an example of the manufacturing method of the pesticide formulation composition of the present invention, it can be expressed that the biopesticide filamentous fungus is cultured in a solid culture medium using at least one species selected from grain seeds, outer skins, bran, and white substances to obtain a cultured After the biopesticide filamentous fungus is attached to the solid culture of the biopesticide filamentous fungus on the solid medium, acetic acid is added to the culture. As an example of a better manufacturing method, it can be expressed that the biopesticide filamentous fungus uses grain seeds or its refined white matter, and solid culture medium that is a granular substance is solid cultured to obtain the cultivated biopesticide filamentous bacteria attached to the granular substance. After solid culture of biopesticide filamentous bacteria in solid medium, acetic acid is supported on the surface of the culture. As an example of a more preferable manufacturing method, it can be expressed that the solid medium of grains or its refined white material is used for the biopesticide filamentous bacteria, and the solid medium is solid culture, so that the cultured biopesticide filamentous bacteria are attached to the pellets. After the solid culture of biological pesticide filamentous bacteria in a solid medium, the surface of the culture is coated with aqueous amorphous silica that adsorbs acetic acid.

The application method of the pesticide formulation composition of the present invention can be appropriately selected according to the types of plants, the types of pests, the application place, the application period, the dosage form, and the like.

The pesticide formulation composition of the present invention can be applied directly, or can be diluted with water or a carrier. Although the method of application can include spreading to plant stems and leaves, spreading to plant heads, spreading to soil surface, soil mixing, soil perfusion, water surface application, seed powder coating, coating, dipping methods, etc., but it is not limited Those who are in such methods. Furthermore, if necessary, the pesticide formulation composition of the present invention can also be mixed, alternately applied, or simultaneously applied with other fungicides, insecticides, nematicides, herbicides, plant growth regulators, fertilizers, soil improvement materials, etc. In this case, there are cases where more excellent effects are shown.

Although the application site of the pesticide formulation composition of the present invention can be applied to seedbeds, farmland, paddy fields, orchards, and hydroponic cultivation facilities for cultivating agricultural and horticultural plants, it is not limited to these.

The application period of the pesticide formulation composition of the present invention is not limited to the planting period. In the case of a nursery, it can be applied before, during, and after planting. In the case of the seedling period, before planting, at the same time as planting, and after planting Can be applied at any period of time.

The application amount of the pesticide formulation composition of the present invention varies depending on the type of plant, the type of pests or weeds, the state of the soil, the application period, the planting density, the formulation, etc., although it cannot be determined in general, for example, It can be used in the nursery period of soil about 100~1000g ^{per 1m 2} respectively, and in the case of nursery, about 5~1000g ^{per 1m 2 of soil can be used.} In addition, in the case of powder coating of seeds (including seed potatoes, tubers, bulbs, bulbs, etc.), the crushed material can be diluted directly or with water, etc., and about 1 to 100 g per kg of seeds are used.

As the plant to be the application target of the pesticide formulation composition of the present invention, specifically, cereals (such as rice, wheat, barley, rye, oats, corn, sorghum, millet, millet, millet, millet, rice, etc.) can be cited. Seeds, buckwheat), potatoes (e.g. potato, sweet potato, taro, yam, konjac), legumes (e.g. soybeans, red beans, kidney beans, peas, broad beans, peanuts, cowpeas, chickpeas, pigeon peas), vegetables (e.g. eggplant, Tomato, green pepper, pepper, cucumber, melon, watermelon, pumpkin, zucchini, squash, gourd, winter melon, bitter gourd, kale, cabbage, broccoli, cauliflower, white radish, turnip, green river vegetable, small pine vegetable, water vegetable, green onion, onion , Leek, garlic, wild garlic, asparagus, lettuce, burdock, chrysanthemum, coltsfoot, carrot, celery, celery, coriander, strawberry, spinach, okra, perilla, basil, mint, ginger, Minghe), fruit trees (such as apples) , Pear, western pear, quince, papaya and crabapple, sweet cherry, peach, plum, plum, apricot, chestnut, walnut, almond, pecan, grape, kiwi, mutong, persimmon, fig, pomegranate, raspberry, blackberry, blueberry , Cranberry, citrus, loquat, olive, bayberry, mango, guava, avocado, date palm, coconut, banana, pineapple, papaya, passion fruit, acerola), special crops (e.g. cotton, flax, rush , Rapeseed, sunflower, sesame, palm oil, sugar beet, sugar cane, tea, coffee, cocoa, hops, tobacco), flowers (such as cosmos, morning glory, marigold, impatiens, gypsophila, sweet peas) , Chrysanthemum, Carnation, Tulip, Lily, Narcissus, Gladiolus, Cyclamen, Begonia, Water Lily, Dali Flower, Rose, Cymbidium, Cattleya), Turfgrass (e.g. Zoysia japonica, Korean grass, Zoysia japonica) Grass, Bermuda grass, bran grass, fescue, ryegrass, bluegrass), trees (e.g. cherry blossoms, rhododendrons) Flowers, oak, beech, cypress, and beech), but not limited to these examples. In addition, it can also be applied to plants that are endowed with tolerance to diseases and pests, tolerance to herbicides, and tolerance to environmental stresses such as drying by classical breeding methods and genetic recombination techniques.

The pesticide formulation composition of the present invention has excellent pest control activity and is useful as a fungicide, nematicide, insecticide, herbicide, plant growth regulator, or soil conditioner. The effects of fungicides, nematicides, and insecticides are not limited to those that show direct control effects on pathogenic bacteria, nematodes, and pests. For example, they also include those that control filamentous fungi, nematodes, and insects by controlling agents. Virus disease prevention and control, indirect prevention and control of the use of biological pesticide filamentous bacteria in the soil to inhibit the proliferation of harmful organisms.

Examples of pathogenic bacteria that can be controlled by the pesticide formulation composition of the present invention include filamentous bacteria, actinomycetes, bacteria, viruses, and viroids. Specifically, bacteria of the genus Ustilago, such as Ustilago nuda, Tilletia, such as Tilletia caries, and Rust (Puccinia) bacteria, such as Puccinia recondita, Gymnosporangium, such as Gymnosporangium asiaticum, Phakopsora, such as soybean rust ( Phakopsora pachyrhizi), Helicobasidium bacteria, such as Helicobasidium mompa, Exobasidium bacteria, such as Exobasidium vexans, Rhizoctonia (Rhizoctonia) bacteria, such as cucumber seedling wilt (Rhizoctonia solani), white sclerotium (Sclerotium) bacteria, such as cucumber and soybean white sclerotium (Sclerotium rolfsii), black spot disease (Phomopsis) bacteria, such as cucumber black Phomopsis sclerotioides, Pyrenochaeta bacteria, such as Pyrenochaeta lycopersici, Septoria, such as Septoria chrysanthe mella, Colletotrichum (Colletotrichum) bacteria, such as strawberry anthracnose (Colletotrichum gloeosporioides), Pestalotiopsis (Pestalotiopsis) genus bacteria, such as Pestalotiopsis longiseta (Pestalotiopsis longiseta), Alternaria (Alternaria) genus bacteria, such as potato summer Blight (Alternaria solani), Botrytis (Botrytis) genus bacteria, such as Botrytis cinerea (Botrytis cinerea), Cercospora (Cercospora) genus bacteria, such as beet brown spot (Cercospora beticola), brown spot disease (Pseudocercospora) Genus bacteria, such as Pseudocercospora vitis (Pseudocercospora vitis), leaf spot disease (Paracercospora) bacteria, such as eggplant brown spot disease (Paracercospora egenula), Cercosporella (Cercosporella) bacteria, such as cabbage white spot disease (Cercosporella brassicae) , Pseudocercosporella (Pseudocercosporella) genus bacteria, such as wheat eye pattern (Pseudocercosporella herpotrichoides), corynespora (Corynespora) genus bacteria, such as cucumber brown spot disease (Corynespora cassiicola), Velcrospora (Mycovellosiella) genus bacteria , E.g. Mycovellosiella nattrassi, Mycovellosiella nattrassi (Passalora) bacteria, such as tomato leaf mold (Passalora fulva), Curvularia (Curvularia) bacteria, such as rice brown rice (Curvularia spp.), Fusarium (Fusarium) bacteria, such as tomato wilt fungus (Fusarium) oxysporum f.sp.lycopersici), Penicillium (Penicillium), for example, Penicillium digitatum, Pyricularia, for example, Pyricularia oryzae, Verticillium , For example, Verticillium dahliae, Taphrina, Taphrina deformans, Blumeria, Blumeria graminis f. sp.tritici), Erysiphe bacteria, such as Erysiphe necator, Sphaerotheca bacteria, such as Sphaerotheca aphanis, Podosphaera bacteria, cucumber Podosphaera xanthii (Podosphaera xanthii), Leveillula (Leveillula) genus bacteria, such as tomato powdery mildew (Leveillula taurica), Leveillula taurica (Calonectria) genus bacteria, such as Soybean black root rot (Calonectria ilicicola), interstitial shell Bacteria of the genus Diaporthe, such as Diaporthe citri, Gaeumannomyces, such as Gaeumannomyces graminis, Rosellinia, such as Pear Rosellinia necatrix, Monosporascus, for example, Monosporascus cannonballus, Elsinoe Bacteria, such as Elsinoe fawcettii, Cryphonectria bacteria, such as Cryphonectria parasitica, Botryosphaeria, such as Botryosphaeria berengeriana , Venturia bacteria, such as Venturia nashicola, Cochliobolus bacteria, such as Cochliobolus miyabeanus, Didymella Genus bacteria, such as Didymella bryoniae (Didymella bryoniae), Sclerotinia (Sclerotinia) genus bacteria, such as Sclerotinia sclerotiorum (Sclerotinia sclerotiorum), Candida (Monilinia) genus bacteria, such as Peach grey scab (Monilinia fructicola) , Rhizopus bacteria, such as Rhizopus spp., Phytophthora bacteria, such as Phytophthora sojae, Pythium bacteria, such as cucumber seedlings Pythium spp., Albugo bacteria, such as Albugo macrospora, Peronospora, such as Peronospora destructor, Bremia ), such as Bremia lactucae (Bremia lactucae), Pseudoperonospora (Pseudoperonospora), such as Cucumber Downy mold (Pseudoperonospora cubensis), Plasmopara (Plasmopara), such as Plasmopara viticola (Plasmopara viticola), silk Aphanomyces bacteria, such as Aphanomyces cochlioides, rhizoma Bacteria of the genus Plasmodiophora, such as Plasmodiophora brassicae, Spongospora, such as Spongospora subterranea, Streptomyces, such as potato scab. Streptomyces spp.), clavibactaer (Clavibactaer) bacteria, such as tomato canker (Clavibactaer michiganensis), soft rot (Pectobacterium) bacteria, such as potato soft rot (Pectobacterium carotovorum), rhizobium (Rhizobium) bacteria, such as rose Rhizobium radiobaeter, Ralstonia bacteria, such as Ralstonia solanacearum, Burkholderia bacteria, such as Burkholderia glumae, acid food Bacteria (Acidovorax) genus bacteria, such as Acidovorax avenae (Acidovorax avenae), Pseudomonas (Pseudomonas) genus bacteria, such as lettuce spoilage bacteria (Pseudomonas spp.), Xanthomonas (Xanthomonas) genus bacteria, such as cabbage black Xanthomonas campestris, fungal baculovirus (Furovirus) genus, such as wheat shattering virus (SBWMV), tobacco mosaic virus (Tobamovirus) genus, such as pepper microspot virus (PMMoV), tobacco rattle virus (Tobravirus) ) Genera, such as tobacco stem wilt virus (TRV), potato X virus (Potexvirus) genera, such as potato X virus (PVX), carnation latent virus (Carlavirus), such as carnation latent virus (CaLV), hairy virus (Crinivirus) ), such as cucurbit chlorosis virus (CCYV), large Wheat yellow mosaic virus (Bymovirus) genus, such as wheat atrophy virus (WYMV), potato Y virus (Potyvirus) genus, such as potato Y virus (PVY), alfalfa mosaic virus (Alfamovirus) genus, such as alfalfa mosaic virus (AMV) , Cucumovirus (Cucumovirus), such as cucumber mosaic virus (CMV), cowpea mosaic virus (Comovirus), such as radish mosaic virus (RaMV), legume virus (Fabavirus), such as broad bean blight virus (BBWV), Nepovirus (Nepovirus) genus, such as tomato round spot virus (TomRSV), Wenzhou mandarin dwarf virus (Sadwavirus) genus, such as Wenzhou shrinking virus (SDV), carnation spot virus (Carmovirus) genus, such as melon wilt spot virus ( MNSV), Ophiovirus (Ophiovirus), such as Tulip Microspot Mosaic Virus (TMMMV), Tenuivirus (Tenuivirus), such as Rice Leaf Blight Virus (RSV), Tomato Spotted Wilt Virus (Tospovirus), such as Tomato Yellow The genus of wilt virus (TSWV), plant reovirus (Phytoreovirus), such as rice shrinking virus (RDV), bean golden mosaic virus (Begomovirus), such as tomato yellow leaf curl virus (TYLCV), cauliflower mosaic (flower Caulimovirus genus, such as Caulimovirus (CaMV), Pospiviroid genus, such as Chrysanthemum Stunting Virus (CSVd), etc., but the present invention is not limited to these examples.

Among the above-mentioned pathogenic bacteria, it is particularly suitable for the genus Pseudomonas purpureus, Rhizoctonia spp., Sclerotium spp., Blackspot spp., Sclerotium spp., Colletotrichum spp., Fusarium spp. Bacteria, Verticillium spp., Lich shell Bacteria, Acrocystis, Pseudomonas spp, Rhizoctonia spp., Sclerotium spp, Sclerotinia, Rhizopus, Phytophthora, Phytophthora Bacteria, Rhizopus spp, Scab spp, Streptomyces spp, Corynebacterium spp, Soft rot spp, Rhizobium spp, Ralstonia solanacearum, Fungal baculovirus, Tobacco Soil infectious pathogens such as mosaic virus, tobacco rattle virus, barley yellow mosaic virus, nematode-borne polyhedral virus, carnation mottle virus, and snake virus are controlled by the present invention.

In addition, as nematodes that can be controlled by the pesticide formulation composition of the present invention, specifically, the genus Aphelenchoides, such as Aphelenchoides besseyi, the genus Bursaphelenchus, such as Bursaphelenchus (Bursaphelenchus xylophilus), Ditylenchus, such as Ditylenchus destructor, Globodera, such as Globodera rostochiensis, Heterodera, such as Soybean Gold Heterodera glycines, Meloidogyne, such as Meloidogyne incognita, Pratylenchus, such as Pratylenchus penetrans, Radopholus, such as Banana (Radopholus similis), Tylenchulus (Tylenchulus), such as Citrus nematode (Tylenchulus semipenetrans), but the present invention is not limited to these examples.

In addition, as the pests that can be controlled by the pesticide formulation composition of the present invention, specific examples include Orthoptera pests, such as Ruspolia lineosa (Ruspolia lineosa), and yellow-faced oil gourd of the Gryllidae family. (Teleogryllus emma), Oriental mole cricket (Gryllotalpa orientalis) of Gryllotalpa orientalis, Oxya yezoensis (Oxya yezoensis), Locusta migratoria (Locusta migratoria), Migratory black locust (Melanoplus sanguinipes), Negative locust ( Atractomorpha lata), Euscyrtus japonicus of the family cricket family, Xya japonicus (Xya japonicus), Thysanoptera pests, such as the Taiwan flower thrips (Frankliniella intonsa) of the thrips family, Western flower thrips (Frankliniella occidentalis), Scirtothrips dorsalis, Thrips palmi, Thrips tabaci, Ponticulothrips diospyrosi, Haplothrips aculeatus, Half Pteran insects, such as Mogannia minuta (Mogannia minuta), Aphrophora intermedia (Aphrophora intermedia), Machaerotypus sibiricus (Machaerotypus sibiricus), Arboridia apicalis), Empoasca onukii, Nephotettix cincticeps, Recilia dorsalis, Pentastiridius apicalis, Spotted Planthopper in Planthopper (Laodelphax striatellus), Nilaparvata lugens, Sogatella furcifera, Nisia nervosa, Kamendaka saccharivora, Achilus flammeus, Orosanga japonicus of the superfamily, Mimophantia maritima (Mimophantia maritima), Cacopsylla pyrisuga (Cacopsylla pyrisuga), Diaphorina citri, Citriidae Calophya mangiferae, Daktulosphaira vitifoliae (Daktulosphaira vitifoliae), Japanese larch aphid (Adelges laricis), Hemlock aphid (Adelges tsugae), Aphididae (Daktulosphaira vitifoliae) Acyrthosiphon pisum), Aphis gossypii, Aphis spiraecola, Lipaphis erysimi, Myzus persicae, Schizaphis graminum, Rhopalosiphum padi, Meal Aleurocanthus camelliae, Aleurocanthus spiniferus, Bemisia tabaci, Bemisia argentifolii, Trialeurodes vaporariorum, Sponge scales Drosicha corpulenta (Drosicha corpulenta), Icerya purchasi, Dysmicoccus brevipes (Dysmicoccus brevipes), Planococcus citri (Planococcus citri), Kang's Pseudococcus comstocki, Ceroplastes ceriferus (Ceroplastes ceriferus), Aclerda takahashii (Aclerda takahashii), Aonidiella aurantii (Aonidiella aurantii) , Pear round scale insect (Diaspidiotus perniciosus), arrow scale insect (Unaspis yanonensis), Lygus lineolaris (Lygus lineolaris), red-bearded fine green transparent wing blindness Trigonotylus caelestialium, Stephanitis pyrioides (Stephanitis pyrioides), Stephanitis nashi (Stephanitis nashi), Eysarcoris aeneus (Eysarcoris aeneus), Rice bug (Lagynotomus elongatus), Rice green Stinkbug (Nezara viridula), Brown-winged Green Stinkbug (Plautia crossota), Megacopta cribraria (Megacopta cribraria), Short-winged Stinkbug (Cavelerius saccharivorus), Malcus japonicus, No. Dysdercus cingulatus (Dysdercus cingulatus), Leptocorisa acuta (Leptocorisa acuta), Leptocorisa chinensis (Leptocorisa chinensis), Anacanthocoris striicornis (Anacanthocoris striicornis) ), Rhopalus maculatus, Cimex lectularis, Coleoptera pests, such as Anomara cuprea, Anomara rufocuprea, Popillia japonica, Oryctes rhinoceros, Agriotes ogurae, Melanotus okinawensis, Melanotus fortnumi, Anthrenus verbasci, Anthrenus verbasci, Anthrenus verbasci Heterobostrychus hamatipennis, Stegobium paniceum (Stegobium paniceum), Pitinus clavipes of Arachnididae, Tenebroides mauritanicus (Tenebroides mauritanicus), Necrobia rufipes (Necrobia rufipes) , Carpophilus hemipterus (Carpophilus hemipterus), Ahasverus hemipterus advena), Cryptolestes ferrugineus (Cryptolestes ferrugineus), Mexican bean lady beetle (Epilachna varivestis) of Coccinellidae, Henosepilachna vigintioctopunctata (Henosepilachna vigintioctopunctata), yellow powder of Coccinellidae Tenebrio molitor, Tribolium castaneum, Epicauta gorhami, Anoplophora glabripennis, Xylotrechus pyrrhoderus, Monochamus alternatus), Callosobruchus chinensis (Callosobruchus chinensis), Leptinotarsa decemlineata (Leptinotarsa decemlineata), Corn root leaf beetle (Diabrotica virgifera), Aulacophora femoralis (Aulacophora femoralis), Brassica chinensis ( Phaedon brassicae), Phyllotreta striolata, Cylas formicarius (Cylas formicarius), Alfalfa postica (Hypera postica), Vegetable weevil (Listroderes costirostris) , Sweet potato weevil (Euscepes postfasciatus), Echinocnemus bipunctatus (Echinocnemus bipunctatus), Rice water weevil (Lissorhoptrus oryzophilus), Corn weevil (Sitophilus zeamais), Hunting weevil Insects (Sphenophrus venatus), etc., Tomicus piniperda (Tomicus piniperda) of the Tomicidae family, Crossotarsus niponicus (Crossotarsus niponicus), Lyctus brunneus (Lyctus brunneus) , Diptera pests, such as Tipula aino (Tipula aino) of the Crane mosquito family, Plecia nearctica (Plecia nearctica), Mycidae Exechia shiitakevora, Pnyxia scabiei (Pnyxia scabiei), Asphondylia yushimai (Asphondylia yushimai), Wheat gall (Mayetiola destructor), Aedes aegypti (Aedes aegypti), Pale Culex pipiens pallens, Simulium takahashii, Chironomus oryzae, Chrysops suavis, Tabanus trigonus, Hoverfly Eumerus strigatus (Eumerus strigatus), Oriental fruit fly (Bactrocera dorsalis), Japanese cherry fruit fly (Euphranta japonica), Mediterranean fruit fly (Ceratitis capitata), Liriomyza gerberus (Liriomyza trifolii), Chromatomyia horticola, Meromyza nigriventris (Meromyza nigriventris), Drosophila suzukii (Drosophila suzukii), Drosophila melanogaster, Water fly Hydrellia griseola, Hippobosca equina, Parallelpmma sasakawae, Delia antiqua, Delia platura, and toilet fly Fannia canicularis (Fannia canicularis), Musca domestica (Musca domestica), Stomoxys calcitrans (Stomoxys calcitrans), Sarcophaga peregrina (Sarcophaga peregrina), Gasterophilus intestinalis (Gasterophilus intestinalis), Hypoderma lineatum (Hypoderma lineatum), Oestrus ovis (Oestrus ovis), Lepidopteran pests, such as Heptiidae (Hypoderma lineatum) (Endoclita excrescens), Antispila ampelopsia (Antispila ampelopsia), Zeuzera leuconotum (Zeuzera leuconotum), Leaf roller (Archips fuscocupreanus), Apple powder horned leaf roller (Adoxophyes orana fasciata), Grapholita molesta, Homona magnanima, Leguminivora glycinivorella, Cydia pomonella, Eupoecilia ambiguella ), Bambalina sp., Eumeta minuscula, Nemapogon granella, Tinea translucens, Bucculatrix pyrivorella), Lyonetia clerkella (Lyonetia clerkella), Caloptilia theivora (Caloptilia theivora), Phyllonorycter ringoniella (Phyllonorycter ringoniella), Phyllocnistis citrella (Phyllocnistis citrella) ), Acrolepiopsis sapporensis (Acrolepiopsis sapporensis), Plutella xylostella (Plutella xylostella), Yponomeuta orientalis (Yponomeuta orientalis), Argyresthia conjugella (Argyresthia conjugella) ), Nokona regalis (Nokona regalis), Phthorimaea operculella (Phthorimaea operculella), Wheat moth (Sitotroga cerealella), Red bollworm (Pectinophora gossypiella), Pectinophora gossypiella, Pectinophora gossypiella, Pectinophora gossypiella Fruit borer (Carposina sasakii), Illiberis pruni (Illiberis pruni), Monema flavescens (Monema flavescens), Grass borer Family Ancylolomia japonica, Chilo suppressalis, Cnaphalocrocis medinalis, Ostrinia furnacalis, Ostrinia nubilalis, Ostrinia nubilalis Cadra cautella (Cadra cautella), wax moth (Galleria mellonella), Nippoptilia vitis (Nippoptilia vitis), Papilio xuthus (Papilio xuthus), Pieris rapae (Pieris rapae) ), Parnara guttata (Parnara guttata), Ascotis selenaria (Ascotis selenaria), Dendrolimus spectabilis (Dendrolimus spectabilis), Malacosoma neustrium testaceum, Sphingidae Agrius convolvuli (Agrius convolvuli), Arna pseudoconspersa (Arna pseudoconspersa), Lymantria dispar (Lymantria dispar), Hyphantria cunea (Hyphantria cunea), Agrotis ipsilon (Agrotis ipsilon) ), Autographa nigrisigna, Tomato Spodoptera (Helicoverpa armigera), Corn Earworm (Helicoverpa zea), Heliothis virescens, Spodoptera exigua, Spodoptera exigua ( Spodoptera litura), Hymenoptera pests, such as Arge pagana (Arge pagana), Chestnut wasp (Apethymus kuri), Red carrageen wasp (Athalia rosae ruficornis), Gallidae Dryocosmus kuriphilus (Dryocosmus kuriphilus), Vespa simillima xanthoptera (Vespa simillima xanthoptera), Fire ant (Solenopsis invicta), and Rose leaf-cutting bee (Megachile) nipponica), Collembola pests, such as Bouletiella hortensis (Bourletiella hortensis), Thysanidae pests, such as Lepisma saccharina, Ctenolepisma villosa, Blattaria pests , Such as the American cockroach (Periplaneta americana) of the family Cockroach family, the German cockroach (Blattella germanica) of the family Cockroach family, isopteran pests, such as the Incisitermes minor (Incisitermes minor) of the woodtermite family, the termites of the family of the rhinotermites (Coptotermes formosanus), Odontotermes formosanus (Odontotermes formosanus), rodent pests, such as Trogium pulsatorium (Trogium pulsatorium), Liposcelis corrodens (Liposcelis corrodens), hair feeding Order pests, such as Lipeurus caponis (Lipeurus caponis), cattle lice (Damalinia bovis) of the animal feather lice family (Damalinia bovis), milk lice pests, such as Haematopinus suis (Haematopinus suis), body lice (Pediculus) humanus), Linognathus setosus (Linognathus setosus), Pthirus pubis (Ptheridae), plant parasitic mites, such as Penthaleus major (Penthaleus major), Trichodidae Phytonemus pallidus, Polyphagotarsonemus latus, Siteroptes sp., Brevipalpus lewisi, Duke Tuckerella pavoniformis (Tuckerella pavoniformis), Tetranychus boreus (Eotetranychus boreus), Orange spider mite (Panonychus citri), Apple red spider (Panonychus ulmi), Tetranychus urticae (Tetranychus urticae) ), Tetranychus Tetranychus kanzawai), Trisetacus pini (Trisetacus pini), Aculops pelekassi (Aculops pelekassi), Epitrimerus pyri, Phyllocoptruta oleivora (Phyllocoptruta oleivora), Diptacus crenatae (Diptacus crenatae), Aleuroglyphus ovatus (Aleuroglyphus ovatus), Tyrophagus putrescentiae (Tyrophagus putrescentiae), Rhizoglyphus robini (Rhizoglyphus robini) The invention is not limited to these examples. Among the above-mentioned pests, particularly soil pests such as Thysanoptera pests, Coleoptera pests, Diptera, Lepidoptera, etc., can be controlled by the present invention.

As the weeds that can be controlled by the pesticide formulation composition of the present invention, weeds of the Onagulaceae family, such as Oenothera erythrosepala, Oenothera laciniata, Ranunculaceae weeds, such as Ranunculus Ranunculus ( Ranunculus muricatus, Ranunculus sardous, Polygonaceae weeds, such as Polygonum convolvulus, Polygonum lapathifolium, Polygonum pensylvanicum, Rumaria, Rumaria crispus persicum ), Rumex obtusifolius, Polygonum cuspidatum (Poligonum cuspidatum), Polygonum pensylvanicum (Polygonum pensylvanicum), Polygonum longiseta (Persicaria longiseta), Persicaria lapathifolia (Persicaria lapathifolia), Polygonum nepalensis (Persicaria nepalensis), Grasses, such as Portulaca oleracea, Caryophyllaceae weeds, such as Stellaria media, Cerastium glomeratum, Stellaria alsine, Spergula arvensis, Stellaria aquatica, Chenopodiaceae weeds, such as Chenopodium album, and foxtail Kochia scoparia, Chenopodium album, Chenopodium ficifolium, Amaranthaceae weeds, such as Amaranthus retroflexus, Amaranthus hybridus, Amaranthus palmeri, False amaranth (Amaranthus spinosus), Amaranthus rudis, Amaranthus albus, Amaranthus viridus, Amaranthus lividus, Cruciferous weeds, such as Raphanus raphanistrum, Wild mustard (Sinapis arvensis), shepherd's purse (Capsellabursa pastoris), Lepidium virginicum, Thlaspi arvense, Descurrinia sophia, Rorippa indica, Rorippa islandica, garlic Mustard (Sisymnrium officinale), Cardamine flexuosa, Watercress (Nasturtium officinale), Draba nemorosa, Leguminous weeds, such as Sesbania exaltata, Cassia obtusifolia, Desmodium tortuosum, Desmodium tortuosum, Vicia sativa, Medicago lupulina, Vicia hirsuta, Kummerowia striata, Medicago polymorpha), Vicia angustifolia, Aeschynomene indica, Malvaceae weeds, such as Abutilon theophrasti, Sida spinosa, Viola weeds, such as Viola arvensis, Viola tricolor, Rubiaceae weeds, such as Galium aparine, Convolvulus weeds, such as Ipomoea hederacea, Ipomoea purpurea, Ipomoea hederacea var integriuscula, Ipomoea lacunosa, Convolvulus arvensis, Ipomoea indica, Ipomoea coccinea, Ipomoea triloba ), Labiatae weeds, such as Lamium purpureum, Lamium amplexicaule, Stachys arvensis, Solanaceae weeds, such as Datura stramonium, Solanum nigrum ), Physalis angulata, Solanum americanum, Solanum carolinense, Scrophulariaceae weeds, such as Veronica persica, Veronica arvensis, Spadeweed (Veronica hederaefolia), Compositae weeds, such as Eclipta prostrata, Bidens tripartita, Xanthium pensylvanicum, Wild sunflower (Helianthus annuus), Chamomile (Matricaria chamomilla), Matricaria (Matricaria perforataorinodora), peacock chrysanthemum (Chrysanthemum segetum), Matricaria matricarioides, Ambrosia artemisiifolia, Ambrosia trifida, Erigeron canadensis, Artemisia princeps, Solidago altissima , Taraxacum officinale, Anthemis cotula, Breea setosa, Sonchus oleraceus, Helianthus tuberosus, Cirsium arvense, Great Wolfgrass (Bidens frondosa), Bidens pilosa, Centurea cyanus, Cirsium vulgare, Lactuca scariola, Rudbeckia hirta, Rudbeckia laciniata , Rudbeckia laciniata var.hortensis Bailey, Senecio vulgais, Silybum marianum, Sonchus asper, Sonchus arvensis, Salsola kali , Madagascar chrysanthemum (Senecio madagascariensis), Coreopsis lanceolata (Coreopsis lanceolata), Boraginaceae weeds, such as Myosotis arvensis, Asclepias weeds, such as Milkweed (Asclepias syriaca), Euphorbiaceae weeds , Such as Euphorbia helioscopia, Euphorbia maculata, Acalypha australis, Acalypha australis, weeds in the gerbera family, such as Geranium carolinianum, weeds in the sorrel family, such as purple flowers Oxalis corymbosa, cucurbitaceous weeds, such as sorrel (Sicyos angulatus), gramineous weeds, such as Echinochloa oryzicola, Leptochloa chinensis, Isachne globosa, Paspalum distichum, Leersia sayanuka, Leersia oryzoides, Echinochloa crus-galli, Setaria viridis, Setaria faberi, Digitaria sanguinalis, Eleusine indica, Poa annua , Alopecurus myosuroides, Avena fatua, Sorghum halepense, Agropyron repens, Bromus tectorum, Cynodone dactylon, Western millet (Panicum dichotomiflorum), Floor millet (Panicum texanum), Sorghum (Sorghum vulgare), Alopecurus geniculatus, Lolium multiflorum, Lolium rigidum, Golden Setaria glauca, Reed grass (Beckmannia syzigachne), Commelinaceae weeds, such as Commelina communis, Murdannia keisak, Equisetum weeds, such as Equisetum arvense, Papaveraceae weeds, such as infants Papaver rhoeas, Cyperaceae weeds, such as Cyperus iria, Cyperus rotundus, Cyperus esculentus, Cyperus difformis, Scirpus hotarui), cattle felt (Eleocharis acicularis), water Cyperus serotinus, Eleocharis kuroguwai, Fimbristylis miliacea, Cyperus flaccidus, Cyperus globosus, Scirpus juncoides, Hydrangea vulgaris (Scirpus wallichii), Scirpus nipponicus, Fimbristylis autumnalis, Scirpus tabernaemontani, Scrophulariaceae weeds, such as Lindernia procumbens, Lindernia dubia, Horsefly Dopatrium junceum, Gratiola japonica, Lindernia angustifolia, Limnophila sessiliflora, Lythraceae weeds, such as Rotala indica, Ammannia multiflora (Ammannia multiflora), Stellaria weeds, such as Elatine triandra, Monochoria vaginalis, Monochoria korsakowii, Heteranthera limosa, and Sagittaria weeds, such as Sagittaria pygmaea, Sagittaria trifolia, Alisma canaliculatum, Sagittaria aginashi, weeds in the Penthouse family, such as Sagittaria trifolia ( Potamogeton distinctus), weeds in the cereal family, such as Eriocaulon cinereum, umbelliferous weeds, such as Oenanthe javanica, etc., but the present invention is not limited to these examples.

Furthermore, with the pesticide formulation composition of the present invention, for example It is necessary to control and control miscellaneous industrial facilities such as slopes of embankments, river beds, shoulders and slopes of roads, railways, parks, squares, parking lots, airports, factories, and storage facilities, fallow land, or idle land in streets. Non-agricultural land for the growth of grass, or a wide range of weeds that can be weeded in tree gardens, pastures, lawns, forestry land, etc. In addition, as objects that can be controlled by the pesticide formulation composition of the present invention, there may be mentioned Chara family growing in rivers, waterways, canals, storage tanks, etc., such as Chara braunii (Chara braunii), duckweed family, for example Waterweed (Spirodela polyrhiza), moss, such as Ricciocarpus natans, Double Star Algae, such as Spirogyra arcla, Yujiiaceae, such as Eichhornia crassipes, Sophoraceae, such as Eichhornia crassipes (Azolla imbricata), Azolla japonica, Salvinia natanas, Araceae, e.g. Pistia stratiotes, Sorphyllaceae, e.g. Myriophyllum aquaticum, etc., but The present invention is not limited to these examples.

Furthermore, in the present invention, the "biological pesticide filamentous bacteria solid culture" and the "acetic acid adsorbent" are only specific structures by displaying the state, and not specific products by the manufacturing method. In addition, the construction of such things for specific operations or analysis other than the foregoing requires excessive economic expenditure or time.

Hereinafter, although the embodiments of the present invention will be described in detail, the present invention is not limited to these embodiments, and various modifications can be made within the technical idea of the present invention.

[Example 1] (Tests on the mycelial growth promoting effect of acetic acid from various filamentous fungal spores)

In mineral salt medium (0.2% NaNO _3, 0.1 mass% KH ₂ PO _4, 0.02 mass% KCl, 0.02 mass% MgSO ₄ .7H ₂ O) will be added in a predetermined concentration were acetic acid as the test medium. For each filamentous fungus of Trichoderma asperelloides SKT-1, Trichoderma harzianum T-22, and Trichoderma hamatum NBRC 31932, the spore suspension (1×10 ⁶ spores) /ml) 0.5ml, plant bacteria into a 100ml Erlenmeyer flask containing 50ml of the test medium, and perform shaking culture (27°C, 120rpm, 3 days). After cultivation, Daguan investigated the growth status of the hyphae of each strain. The results of these tests are shown in Table 1. In addition, the same test was also performed on Talaromyces flavus SAY-Y-94-01 strain and Metarhizium anisopliae SMZ-2000 strain. Still, Trichoderma asperelloides SKT-1 strain is sold as a pesticide under the trade name of Ecohope (registered trademark, manufactured by Combina Chemical Industry Co., Ltd.). Trichoderma harveyi T-22 strain is sold under the trade name of Trichodoisole (registered trademark, manufactured by Arysta Life Science Co., Ltd.) as a soil improvement material. Trichoderma uncariae NBRC 31932 strain is sold separately by the independent administrative legal person product evaluation technology base agency. The yellow basket-shaped bacteria SAY-Y-94-01 strain is sold as a pesticide under the trade name of Tough block (registered trademark, manufactured by Idemitsu Kosan Co., Ltd.). The Metarhizium anisopliae SMZ-2000 strain is sold as a pesticide under the trade name of Pirates granules (registered trademark, manufactured by Arysta Life Science Co., Ltd.).

As a result, in the spores of Trichoderma spores, the growth of hyphae was not observed at an acetic acid concentration of about 0.15 to 0.3% by mass, which means that even if the spores are simply mixed with acetic acid above a certain concentration, growth is difficult. In addition, although the spores of SAY-Y-94-01 strain and Metarhizium anisopliae SMZ-2000 strain differ somewhat in the mycelial growth status of each acetic acid concentration, they are roughly the same as those of Trichoderma The genus bacteria have the same result.

[Example 2] (Preservation stability test of pesticide formulation composition)

As a pesticide formulation composition blended with acetic acid, the acetic acid base powder coating formulation and the acetic acid direct spray formulation as the product of the present invention were produced, and the SKT-1 strain liquid formulation as a comparison product (trade name: "Ecohope", Combina Chemical Industry) Co., Ltd.) 3 preparations mixed with 15% by volume of acetic acid preparations. The acetic acid matrix powder coating formulation is to absorb acetic acid on white carbon black to become 15% by mass matrix powder, cultured with SKT-1 strain of barley seed medium (containing 8g of barley seeds (variety: Zaotake 2), YG medium (0.3 Mass% yeast extract, 2.0 mass% glucose) 8ml 100ml Erlenmeyer flask planting bacteria SKT-1 strain spore suspension (1×10 ⁶ spores/ml) 0.5ml, after standing culture at 25℃ for 8 days, Naturally air-dried overnight. Hereinafter, it is also referred to as SKT-1 strain solid culture): The ratio of the aforementioned substrate powder = 99:1 (mass ratio) is covered by the sufficient mixing in the plastic bag. The direct injection of acetic acid is a solution of 15% by mass by diluting acetic acid in water. The solid culture of the SKT-1 strain: the aforementioned solution=99:1 (mass ratio) is mixed by vibration in the plastic bag. One solid culture was sprayed with the aforementioned solution to adsorb acetic acid. After preparation, the preparation was packaged in an aluminum bag and stored at 5°C. After 0 days (initial value), 1 day, and 7 days, the number of bacteria in the preparation was measured by the dilution plate method. The results of this test are shown in Table 2.

As a result, the acetic acid matrix powder-coated preparation and the acetic acid direct spray preparation of the filamentous bacteria solid culture of the product of the present invention can survive a certain degree of filamentous bacteria even after 7 days of manufacture. The filamentous bacteria liquid is simply mixed with acetic acid. For comparison products, the number of bacteria immediately after manufacture (initial value) is also higher than that of the present invention There are fewer products, and the survival of filamentous bacteria has not been observed 1 day after manufacture.

[Example 3] (Test on the control effect of cucumber sclerotiasis of pesticide formulation composition)

Sclerotium rolfsii (Sclerotium rolfsii) was pre-cultured on a PDA plate medium, and the agar-containing slices were mixed in a soil wheat bran medium (sandy loam 2.5kg, wheat bran 2.0kg, yeast extract 3g, glucose 20g, 1L of distilled water), cultured at 27°C for 7 days. The inoculation source and the soil (sterilized sandy loam: horticultural soil (trade name: "Combined Nippi horticultural soil No. 1", manufactured by Nippon Fertilizer Co., Ltd.) = 1:1 (volume ratio)) are 1:5 (volume ratio) ) Is fully mixed, and placed in a wet room (27°C, dark place) for one night is regarded as contaminated soil. Spread about 15 ml of this contaminated soil on the plant heads of the cotyledon stage cucumber (variety: day branch) grown in a plastic cup (diameter 6 cm) for inoculation. After inoculation, the acetic acid matrix powder coating preparation and the acetic acid direct spray preparation of Example 2 and the SKT-1 strain solid culture system are spread equivalent to 500g/m ² on the cucumber plant head, and the SKT-1 strain liquid formulation will be SKT- 1 plant of liquid: water = 1:14 (mass ratio) diluted dispersion liquid perfusion is equivalent to 3L/m ² in the head of cucumber plant, tolclofos methyl hydrate (trade name: "Rysorex hydrate" ( registered trademark), manufactured by Sumitomo chemical Co., Ltd.) will tolclofos-based hydrating agent: the dispersion was diluted 999 ratio (mass ratio) corresponds perfusion 3L / m ² on cucumber plants head: water = 1. After the drug treatment, after 3 days of management in a wet room (27°C, dark place), the presence or absence of the disease was investigated, and the control price was calculated by the following calculation formula. The results of this test are shown in Table 3.

Control price = (1-Diseased plant rate in the treated area / Diseased plant rate in the untreated area) × 100

From this result, it is understood that the acetic acid base powder coating preparation and the acetic acid direct spray preparation of the product of the present invention show the same level of control effect as the organophosphorus-based fungicide of tolclophos-methyl hydrate, and show better performance against cucumber white sclerotia. SKT-1 strain solid culture or SKT-1 strain liquid agent has higher control effect.

[Example 4] (Experiment on the control effect of cucumber sclerotiae based on the difference in the treatment amount of the pesticide formulation composition)

By the same method as in Example 3, the acetic acid-based powder coating preparation was tested for the control effect of cucumber white sclerotia. Incidentally, the amount of acetic acid base powder coating formulation to be spread was a multiple of that in Example 3. Table 4 compares the results of this test with the data of Example 3.

As a result, even if the treatment amount of the acetic acid-based powder-coated preparation of the present invention is increased to double the amount, a sufficient control effect against cucumber sclerotiasis is observed, but the control effect of the treatment amount dependence is not observed. Promote.

[Example 5] (Preservation stability and prevention of cucumber white sclerotia by the difference of the acetic acid blending amount of the pesticide formulation composition)

Acetic acid is adsorbed on white carbon black to become a matrix powder of 50% by mass, 15% by mass or 5% by mass. By using the solid culture of the SKT-1 strain: the aforementioned matrix powder = 99:1 (mass ratio) The inside of the plastic bag was fully mixed and coated to prepare an acetic acid matrix powder coating formulation with an acetic acid blending amount of 0.5% by mass, 0.15% by mass, or 0.05% by mass, respectively. After being stored at 4°C for 1 week, the number of bacteria in the preparation and the control effect of cucumber white sclerotia were investigated. The number of bacteria in the preparation was investigated by the same method as in Example 2, and the control effect of cucumber white sclerotiae was investigated by the same method as in Example 3. The test results of the number of bacteria in the preparation are shown in Table 5, and the test results of the control effect of cucumber sclerotiae are shown in Table 6.

As a result, it was found that when the blending amount of acetic acid was 0.05 to 0.5% by mass, no significant decrease in the number of bacteria during storage was observed, and the control effect of cucumber sclerotiae was also at the same level as that of tolclofos-methyl hydrate.

[Example 6] (Pesticide formulation composition of cucumber seedling wilt (Rhizoctonia) control effect)

After the cucumber seedling Rhizoctonia solani AG-4 was pre-cultured on a PDA plate medium, the agar-agar slices containing the bacteria were mixed in a soil wheat bran medium and cultured at 27°C for 7 days. Mix the inoculation source with soil (sterilized sandy loam soil: horticultural culture soil = 1:1 (volume ratio)) in a ratio of 1:50 (volume ratio), and place it in a wet room (27°C, dark place) overnight. To pollute the soil. Fill a corner bowl (9cm square) with 200ml of sterilized Sichuan sand and 100ml of contaminated soil in sequence. Still, instead of contaminated soil, the non-inoculated flora is filled with soil wheat bran medium: soil = 1:50 (volume ratio) mixed with 100ml. The acetic acid matrix powder coating formulation of Example 2 (acetic acid blending amount 0.15%) was mixed with soil. Before filling the contaminated soil in a diagonal bowl, 100ml of the contaminated soil was mixed with 8.1g of the acetic acid matrix powder coating formulation (equivalent to 1000g/m ² ) Was mixed in a plastic bag and filled into a corner bowl for irrigation, and then sowed cucumbers (variety: Nissan). The surface layer of the acetic acid matrix powder coating preparation is scattered. After irrigation, the cucumber seeds are sown on the contaminated soil, and the equivalent of 500g/m ^{2 is} uniformly scattered on the surface of the soil. ^{As a comparison, the dispersion solution equivalent to 3L/m 2} diluted with tolclofos methyl hydrate: water=1:499 (mass ratio) was subjected to soil infusion treatment before sowing. After chemical treatment, it was placed in a wet room (27℃, dark place) for one night, and then managed in the greenhouse (overhead irrigation). Eight days after vaccination, an index survey was conducted based on the following disease index standard. In addition, the disease degree, corrected disease degree, and control price were calculated by the following formulas. The results of this test are shown in Table 7.

<Incidence Index>

0: No disease

1: Abnormal fertility

2: Withered or not budding

Incidence degree = Σ (number of diseased seedlings by degree × index) / (number of seedlings investigated × 2) × 100

Modified incidence = average incidence of each district-average incidence of no vaccination area

Prevention price = (1-corrected incidence of treatment area/corrected incidence of no treatment area)×100

From this result, it is understood that the acetic acid-based powder coating preparation of the present invention shows the same degree of control as tolclofos-methyl hydrate against cucumber seedling wilt (Rhizoctonia), soil mixing treatment and soil surface treatment. effect.

[Example 7] (Control effect of cucumber seedling wilt (Pythium) of pesticide formulation composition)

After the cucumber seedling Pythium aphanidermatum was pre-cultured on the PDA plate medium, the agaric slices containing the bacteria were mixed in the soil wheat bran medium and cultured at 27°C for 7 days. Mix this inoculation source with soil (sterilized sandy loam soil: horticultural culture soil = 1:1 (volume ratio)) in a ratio of 1:300 (volume ratio), and place it in a wet room (27°C, dark place) overnight. To pollute the soil. Fill a corner bowl (9cm square) with 200ml of sterilized Sichuan sand and 100ml of contaminated soil in sequence. Still, instead of the contaminated soil, the non-inoculated flora is filled with soil wheat bran medium: soil=1:300 (volume ratio) mixed with 100ml. The acetic acid matrix powder coating formulation of Example 2 (acetic acid blending amount 0.15% by mass) was mixed with soil. Before filling the contaminated soil diagonally, 100ml of the contaminated soil was mixed with 4.1g of the acetic acid matrix powder coating formulation (equivalent to 500g/m ² ) After mixing in a plastic bag and filling in a corner bowl for irrigation, sowing cucumbers (variety: daily branch). Hydroxyisoxazole liquid (trade name: "Tachigaren liquid" (registered trademark), manufactured by Mitsui Chemicals Agro Co., Ltd.) is diluted with hydroxyisoxazole liquid: water=1:499 (volume ratio) It is equivalent to 3L/m ² of the dispersing liquid to be poured into the soil after sowing. After chemical treatment, it was placed in a wet room (27°C, dark place) for one night, and then managed in a greenhouse. Twelve days after vaccination, an index survey was conducted based on the following disease index standards, and the disease severity, corrected disease severity, and control price were calculated using the following mathematical formulas. The results of this test are shown in Table 8.

<Incidence Index>

0: No disease

1: Abnormal fertility

2: Withered or not budding

Incidence = Σ (number of diseased seedlings by degree × index) / (number of seedlings investigated × 2) × 100

Modified incidence = average incidence of each district-average incidence of no vaccination area

Prevention price = (1-corrected incidence of treatment area/corrected incidence of no treatment area)×100

As a result, it was found that the acetic acid-based powder coating preparation of the product of the present invention was treated with soil mixing to show the same level of control effect as that of hydroxyisoxazole liquid against cucumber seedling wilt (Pythium).

[Example 8] (Control effect of cucumber sclerotium disease by the difference of various grain culture media of pesticide formulation composition)

As the test grains, rice seeds (variety: Serenguang), wheat seeds (variety: Nonglin 61), barley seeds (variety: Zaochu 2), soybean seeds (variety: Yanli), rice bran, and wheat bran were used. (Wheat bran). In a 100ml Erlenmeyer flask containing 8g of various grains, 8ml of YG medium (0.3% by mass yeast extract, 2.0% by mass glucose) ^{, 0.5ml of spore suspension of SKT-1 strain (1×10 6} spores/ml), Incubate at 25°C for 8 days. After culturing and air-drying overnight, the SKT-1 strain culture and the matrix powder containing 15% by mass of acetic acid adsorbed on white carbon black are applied to the plastic at a ratio of 99:1 (mass ratio). The inside of the bag is fully mixed and coated to prepare an acetic acid-based powder coating preparation. With respect to these preparations, the same method as in Example 3 was used to investigate the control effect of cucumber white sclerotia. The results of this test are shown in Table 9.

As a result, it was found that the preparation containing the SKT-1 strain solid culture obtained by using various grains as a solid medium and acetic acid showed the same level or more control effect as tolclofos-methyl hydrate for cucumber sclerotia.

The synthesis of the present invention is as follows.

The present invention provides a biopesticide filamentous fungus with a pesticide active ingredient that has the effect of preventing pests and/or weeds and regulating the growth of plants, and provides a microbial pesticide formulation composition that is highly activated by acetic acid. A microbial pesticide preparation composition that can be stored for a long period of time, its manufacturing method, the method of controlling pests or weeds by the use, the method of plant growth adjustment, etc.

Furthermore, by using a biopesticide filamentous fungus solid culture containing at least one species selected from grain seeds, their hulls, bran, and whites as a solid medium, and a pesticide preparation composition formed with acetic acid, the above is solved Subject.

[Trusted Number]

The consignment numbers of microorganisms that will be deposited internationally in the present invention are shown below.

(1) Trichoderma asperelloides SKT-1 strain (FERM BP-16510).

Claims (24)

A pesticide preparation composition containing a solid culture of biological pesticide filamentous fungus and acetic acid. The solid culture of biological pesticide filamentous fungus will be selected from the group consisting of grain seeds, skins, bran, and whites. The above is used as a solid medium. Such as the pesticide formulation composition of claim 1, wherein the biological pesticide filamentous fungus has the function of preventing pests and/or weeds, and the filamentous fungus of the plant growth adjustment function. For example, the pesticide formulation composition of claim 2, wherein the filamentous fungus having the control effect on pests and/or weeds and the plant growth adjustment effect belongs to the genus Beauveria, Scutellaria ( Coniothyrium, Metarhizium, Talaromyces, Trichoderma, Verticillium. For example, the pesticide formulation composition of claim 3, wherein the filamentous fungus having the effect of controlling pests and/or weeds and adjusting the growth of plants is Trichoderma. Such as the pesticide formulation composition of claim 4, wherein the Trichoderma is asperelloides species, asperellum species, and dark green wood Any of atroviride species, hamatum species, harzianum species, and koningii species. Such as the pesticide formulation composition of claim 5, wherein the Trichoderma is a species of asperelloides. Such as the pesticide formulation composition of claim 6, wherein the asperelloides strain is Trichoderma asperelloides SKT-1 strain (FERM BP-16510). For example, the pesticide formulation composition of any one of Claims 1 to 7, which is contained in 1 g of the pesticide formulation composition and contains ^{10-10 10} CFU (Colony Forming Unit) of biological pesticide filamentous bacteria. Such as the pesticide formulation composition of claim 1 or 2, wherein the solid medium is the white substance of grain seeds. Such as the pesticide formulation composition of claim 1 or 2, wherein the grain is a gramineous grain. Such as the pesticide formulation composition of claim 10, wherein the gramineous grain is barley. Such as the pesticide formulation composition of claim 1 or 2, wherein the content of acetic acid is 0.01 to 1% by mass in the pesticide formulation composition. For example, the pesticide preparation composition of claim 1 or 2 is a granular pesticide preparation composition, and the solid medium is a granular substance. Such as the pesticide formulation composition of claim 13, wherein the acetic acid is an acetic acid adsorbent formed by adsorbing acetic acid on a fine mineral powder. Such as the pesticide formulation composition of claim 14, wherein the fine mineral powder is aqueous amorphous silica. Such as the pesticide formulation composition of claim 14 or 15, wherein the content of the acetic acid adsorbent is 0.5-2% by mass in the pesticide formulation composition. A method for producing a pesticide formulation composition, which is characterized in that the biopesticide filamentous fungus uses one or more selected from grain seeds, outer skins, bran, and whites as a solid medium for solid culture to obtain a cultured biopesticide After the filamentous bacteria are attached to the solid culture of the biological pesticide filamentous bacteria on the solid medium, acetic acid is added to the culture. According to the method of claim 17, wherein the pesticide formulation composition is a granular substance, and the solid medium is a granular substance. Such as the method of claim 17 or 18, which is carried on the surface of the solid culture of biological pesticide filamentous bacteria by spraying acetic acid or diluting the acetic acid with water or an organic solvent to carry acetic acid. According to the method of claim 18, the acetic acid is adsorbed on the fine mineral powder, and the surface of the solid culture of the biopesticide filamentous fungus is coated with the adsorbent. Such as the method of claim 20, wherein the mineral powder is hydrous amorphous silicon dioxide. A method for controlling and/or adjusting plant growth of one or more species selected from weeds, diseases, and pests, which is characterized in that the pesticide formulation composition according to any one of claims 1 to 16 is applied to plant seedlings and seedlings Or seeds, and cultivate the plant. A method for controlling and/or adjusting plant growth of one or more species selected from the group consisting of weeds, diseases, and pests, which is characterized in that the pesticide formulation composition according to any one of claims 1 to 16 is applied to the soil for cultivating plants . Such as the method of claim 23, which is to apply 5~1000g of pesticide formulation composition ^{per 1 m 2 of soil.}