WO2017188049A1 - Composition for microbial pesticide formulation, method for producing same, and method for use thereof - Google Patents
Composition for microbial pesticide formulation, method for producing same, and method for use thereof Download PDFInfo
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- WO2017188049A1 WO2017188049A1 PCT/JP2017/015504 JP2017015504W WO2017188049A1 WO 2017188049 A1 WO2017188049 A1 WO 2017188049A1 JP 2017015504 W JP2017015504 W JP 2017015504W WO 2017188049 A1 WO2017188049 A1 WO 2017188049A1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
- A01N63/38—Trichoderma
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M21/00—Apparatus for the destruction of unwanted vegetation, e.g. weeds
- A01M21/04—Apparatus for destruction by steam, chemicals, burning, or electricity
- A01M21/043—Apparatus for destruction by steam, chemicals, burning, or electricity by chemicals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M7/00—Special adaptations or arrangements of liquid-spraying apparatus for purposes covered by this subclass
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M9/00—Special adaptations or arrangements of powder-spraying apparatus for purposes covered by this subclass
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/34—Shaped forms, e.g. sheets, not provided for in any other sub-group of this main group
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/02—Acyclic compounds
Abstract
Description
(1)穀物の種子、その外皮、糠、精白物から選ばれる1以上を固体培地とした生物農薬糸状菌固体培養物と、酢酸を含有してなる農薬製剤組成物。
(2)生物農薬糸状菌が、病害虫及び/又は雑草に対する防除作用、並びに、植物成長調整作用を有する糸状菌である(1)に記載の農薬製剤組成物。
(3)病害虫及び/又は雑草に対する防除作用、並びに、植物成長調整作用を有する糸状菌が、ボーベリア(Beauveria)属、コニオチリウム(Coniothyrium)属、メタリジウム(Metarhizium)属、タラロマイセス(Talaromyces)属、トリコデルマ(Trichoderma)属、バーティシリウム(Verticillium)属のいずれかに属する菌である(2)に記載の農薬製剤組成物。
(4)病害虫及び/又は雑草に対する防除作用、並びに、植物成長調整作用を有する糸状菌が、トリコデルマ属菌である(3)に記載の農薬製剤組成物。
(5)トリコデルマ属菌が、アスペレロイデス(asperelloides)種菌、アスペレラム(asperellum)種菌、アトロビリデ(atroviride)種菌、ハマタム(hamatum)種菌、ハルジアナム(harzianum)種菌、コニンギ(koningii)種菌のいずれかである(4)に記載の農薬製剤組成物。
(6)トリコデルマ属菌が、アスペレロイデス種菌である(5)に記載の農薬製剤組成物。
(7)アスペレロイデス種菌が、トリコデルマ・アスペレロイデス(Trichoderma asperelloides) SKT-1株(FERM BP-16510)である(6)に記載の農薬製剤組成物。
(8)農薬製剤組成物中1g中に生物農薬糸状菌が10~1010CFU(Colony Forming Unit)含まれることを特徴とする(1)~(7)のいずれか1つに記載の農薬製剤組成物。
(9)固体培地が、穀物の種子の精白物である(1)~(8)のいずれか1つに記載の農薬製剤組成物。
(10)穀物が、イネ科穀物である(1)~(9)のいずれか1つに記載の農薬製剤組成物。
(11)イネ科穀物が、大麦である(10)に記載の農薬製剤組成物。
(12)酢酸の含量が、農薬製剤組成物中0.01~1質量%、例えば0.04~0.6質量%である(1)~(11)のいずれか1つに記載の農薬製剤組成物。
(13)粒状農薬製剤組成物であって、固体培地が粒状物であることを特徴とする(1)~(12)のいずれか1つに記載の農薬製剤組成物。
(14)酢酸が、鉱物質微粉に酢酸が吸着されてなる酢酸吸着物である(13)に記載の農薬製剤組成物。
(15)鉱物質微粉が、含水非晶質二酸化珪素である(14)に記載の農薬製剤組成物。
(16)酢酸吸着物の含量が、農薬製剤組成物中0.5~2質量%である(14)又は(15)に記載の農薬製剤組成物。
(17)生物農薬糸状菌を、穀物の種子、その外皮、糠、精白物から選ばれる1以上を固体培地として用いて固体培養し、培養した生物農薬糸状菌が固体培地に付着する生物農薬糸状菌固体培養物を得た後、該培養物に酢酸を添加することを特徴とする農薬製剤組成物の製造方法。
(18)農薬製剤組成物が粒状物であって、固体培地が粒状物であることを特徴とする(17)に記載の方法。
(19)生物農薬糸状菌固体培養物の表面に、酢酸又は酢酸を水もしくは有機溶媒で希釈した溶液を噴霧することにより酢酸を担持させることを特徴とする(17)又は(18)に記載の方法。
(20)酢酸を鉱物質微粉に吸着させ、生物農薬糸状菌固体培養物の表面に該吸着物を被覆することを特徴とする(18)に記載の方法。
(21)鉱物質微粉が、含水非晶質二酸化珪素である(20)に記載の方法。
(22)(1)~(16)のいずれか1つに記載の農薬製剤組成物を、植物の苗、苗木又は種子に施用し、該植物を栽培することを特徴とする、雑草、病害、害虫から選ばれる1以上の防除及び/又は植物成長調整方法。
(23)(1)~(16)のいずれか1つに記載の農薬製剤組成物を、植物を栽培する土壌に施用することを特徴とする、雑草、病害、害虫から選ばれる1以上の防除及び/又は植物成長調整方法。
(24)土壌1m2あたり農薬製剤組成物を5~1000g、例えば100~1000g施用することを特徴とする(23)に記載の方法。 That is, the embodiment of the present invention is as follows.
(1) A biopesticide filamentous fungus solid culture using at least one selected from cereal seeds, its hulls, straws, and refined products as a solid medium, and an agrochemical formulation composition containing acetic acid.
(2) The agrochemical formulation according to (1), wherein the biopesticide filamentous fungus is a filamentous fungus having a controlling action against pests and / or weeds and a plant growth regulating action.
(3) Filamentous fungi having a controlling action against pests and / or weeds and a plant growth regulating action include the genus Beauveria, the genus Coniothyrium, the genus Metarhizium, the genus Talaromyces (Talaromyces), Trichoderma The agrochemical formulation composition according to (2), which is a bacterium belonging to any of the genus Trichoderma) and the genus Verticillium.
(4) The agrochemical formulation according to (3), wherein the filamentous fungus having a controlling action against pests and / or weeds and a plant growth regulating action is Trichoderma sp.
(5) The genus Trichoderma is an Asperelloides inoculum, an Asperellum inoculum, an atroviride inoculum, a hamatum inoculum, a harzianum inoculum, or Koningii (Koningii inoculum) ) Agrochemical formulation composition.
(6) The agrochemical formulation according to (5), wherein the Trichoderma spp. Is Aspereroides sp.
(7) The agrochemical formulation according to (6), wherein the Aspereroides inoculum is Trichoderma aspereloides SKT-1 strain (FERM BP-16510).
(8) The agrochemical formulation according to any one of (1) to (7), wherein 10 to 10 10 CFU (Colony Forming Unit) of the biopesticide filamentous fungus is contained in 1 g of the agrochemical formulation composition. Composition.
(9) The agrochemical formulation according to any one of (1) to (8), wherein the solid medium is a refined product of cereal seeds.
(10) The agrochemical composition according to any one of (1) to (9), wherein the cereal is a gramineous cereal.
(11) The agrochemical formulation according to (10), wherein the gramineous grain is barley.
(12) The agrochemical formulation according to any one of (1) to (11), wherein the acetic acid content is 0.01 to 1% by mass, for example, 0.04 to 0.6% by mass in the agrochemical formulation composition. Composition.
(13) The agrochemical composition according to any one of (1) to (12), wherein the composition is a granular agrochemical formulation, and the solid medium is a granule.
(14) The agricultural chemical preparation composition according to (13), wherein the acetic acid is an acetic acid adsorbent obtained by adsorbing acetic acid on a mineral fine powder.
(15) The agrochemical formulation according to (14), wherein the fine mineral powder is hydrous amorphous silicon dioxide.
(16) The agricultural chemical formulation composition according to (14) or (15), wherein the content of the acetic acid adsorbent is 0.5 to 2% by mass in the agricultural chemical formulation composition.
(17) Biopesticide filamentous fungi in which the biopesticide filamentous fungus adheres to the solid medium by solid-culturing the biopesticide filamentous fungus using one or more selected from cereal seeds, hulls, cocoons, and refined products as a solid medium A method for producing an agrochemical formulation composition comprising adding a solid culture of a fungus and then adding acetic acid to the culture.
(18) The method according to (17), wherein the agricultural chemical preparation composition is a granular material, and the solid medium is a granular material.
(19) The acetic acid is supported on the surface of the biopesticide filamentous fungus solid culture by spraying acetic acid or a solution obtained by diluting acetic acid with water or an organic solvent, as described in (17) or (18) Method.
(20) The method according to (18), wherein acetic acid is adsorbed on the fine mineral powder, and the adsorbate is coated on the surface of the biopesticide filamentous fungus solid culture.
(21) The method according to (20), wherein the mineral fine powder is hydrous amorphous silicon dioxide.
(22) Weeds, diseases, characterized by applying the agrochemical formulation composition according to any one of (1) to (16) to plant seedlings, seedlings or seeds and cultivating the plants, One or more control and / or plant growth adjustment methods selected from pests.
(23) One or more control agents selected from weeds, diseases, and pests, characterized in that the agrochemical formulation composition according to any one of (1) to (16) is applied to soil where plants are grown. And / or plant growth regulation method.
(24) The method according to (23), wherein 5 to 1000 g, for example, 100 to 1000 g of the agrochemical composition is applied per 1 m 2 of soil.
上記病原菌のうち、特に、ヘリコバシディウム属菌、リゾクトニア属菌、スクレロティウム属菌、ホモプシス属菌、ピレノケータ属菌、コレトトリカム属菌、フザリウム属菌、バーティシリウム属菌、カロネクトリア属菌、ゴウマノマイセス属菌、ロゼリニア属菌、モノスポラスカス属菌、ディディメラ属菌、スクレロティニア属菌、リゾープス属菌、ファイトフトラ属菌、アファノマイセス属菌、プラスモジオフォラ属菌、スポンゴスポラ属菌、ストレプトマイセス属菌、クラビバクター属菌、ペクトバクテリウム属菌、リゾビウム属菌、ラルストニア属菌、フロウイルス属、トバモウイルス属、トブラウイルス属、バイモウイルス属、ネポウイルス属、カルモウイルス属、オフィオウイルス属などの土壌伝染性病原菌を本発明により好適に防除できる。 Examples of pathogenic bacteria that can be controlled by the agrochemical formulation composition of the present invention include filamentous fungi, actinomycetes, bacteria, viruses, viroids, and the like. Specifically, the genus Ustylago, for example, Barley bare smut fungus (Ustilago nuda), the genus Tilletia (eg, Tilletia caries), the genus Puccinia scab (eg, fungus) (Puccinia recondita), Gymnosporangium spp., For example Gymnosporadium asiaticum, Phacosoporico spp., For example soybean rust Asparagus purple crest fungus (Helicobasidium mom) a), genus Exobasidium, such as Chamochi fungus (Exobasidium vexans), genus Rhizoctonia, such as Rhizoctonia solani, sclerotium Cucumber and soybean white silk fungus (Sclerotium rolfsii), genus Homopsis (eg, cucumber homoposis root rot fungus), Pyrenochaeta (eg, Pyrenochaeta), eg, tomato brown root rot (S) ) Genus fungi, for example, Septoria chr santhemella, Colletotrichum spp., for example, Strawberry anthracnose fungus (Colletotrichum gloeosporioides), Pestalothiopteris spp., for example, Pestarotropis algae spp. (Alternaria solani), genus Botrytis, such as cucumber gray mold fungus (Botrytis cinerea), genus Cercospora, such as sugar beet fungus (Cercospora beticola), Pseudosor spo Pseudocercospora vitis, Paracercospora genus, such as eggplant brown rot (Paracercospora genula), Cercosporella genus, such as cercosporacosporacer ) Genus fungi, such as wheat eye spot disease fungus (Pseudocercosporella herpotriochoides), Corynespora spp., Eg cucumber brown spot fungus (Corynespora casicola), Mycoberosiella spp. Mycovellosiella natrassi, genus Passalora, for example, Tomato leaf mold (Passalora fulva), genus Curvularia, for example rice brown rice (Curvularia spp. ), Fusarium spp., For example, Fusarium oxysporum f. Sp. Lycopersici, Penicillium spp., For example, Penicillium digitatum, Pyricularia, Pirularia spp. Pyricularia oryzae, Verticillium spp., Such as eggplant half-wilt fungus (Verticillium dahlia), Taphrina spp., Peach currant fungus (Taphrina deformans), erum Powdery mildew (Blumeria graminis f. Sp. Tr tici), Erysiphe genus bacteria such as grape powdery mildew (Erysiphe necator), Sphaerotheca genus bacteria such as strawberry powdery mildew (Sphaerotheca aphanis), Podosphaera (Podosphaera) xanthii), Leveillula spp. such as tomato powdery mildew (Leveillula taurica), Caronectria spp. such as soybean black root rot fungi (Calonectria licicola), Diaporte (Diapolet fungus), Diaporthe citri), Gaumanomyces (Gaeumanomyces), for example, wheat stem blight (Gaeumanomyces graminis), Rosellinia (eg, Rosellinia necatrix), Monosporacus (Musporascus), Monosporacus cannonballus, Elsinoe genus, for example Elsinoe factocetii, Cryphonectria genus, for example Cryphonectria paritia parasite, , For example, Botryosphae ia berengeria, genus Venturia, for example, Venturia naschicola, genus Cochliobolus, such as rice sesame leaf blight fungus (Cochliobolus miyabeanus), diploid bacterium D (Didymella broniae), genus Sclerotinia, eg, cabbage sclerotia, Monilia genus, eg, Monilinia frizoius, eg, Monilinia phricus Rhizopus spp. ), Phytophthora spp., For example, Phytophthora sojae, Pythium spp., For example, Phythium spp., Albgo spp. Bacteria (Albgo macrospora), Peronospora (eg Peronospora destructor), Bremia (eg, Bremia lactoporodeporporus), Pseudospora peus For example, Cucumber downy mildew (Pseudoperonospora cubensis), Plasmopara (Plasmopara) genus E.g., Plasmopara viticola, Aphanomyces, e.g., Aphanomyces cochlioides, e.g. Plasmodiophora, e.g., Plasmophora Spongospora spp., For example potato powder scab, Streptomyces spp., Streptomyces spp., Clavibacter tomato, Clavibacter spp. Clavibacter mi higanensis, Pectobacterium, such as potato soft rot, Rhizobium, such as Rhizobium ratobacter, Rhizobium ratobacter Ralstonia solanacerum, Burkholderia genus bacteria, for example, Burkholderia glumae, Acidoborax genus bacteria, for example, Rice brown moss ) Genus, for example lettuce rot fungus Pseudomonas spp. ), Xanthomonas spp., Such as cabbage black rot fungus (Xanthomonas campestris), Frovirus (Furovirus), eg, wheat dwarf virus (SBWMV), Tobamovirus (Tobamovirus), Tobravirus genus such as tobacco stem virus (TRV), Potexvirus genus such as potato X virus (PVX), Carlavirus genus such as carnation latent virus (CaLV), Clini Virus genus, for example, cucurbit yellowing virus (CCYV), genus Bymovirus, eg For example, wheat stripe dwarf virus (WYMV), Potyvirus, eg Potato virus Y (PVY), Alfavirus, eg Alfalfa mosaic virus (AMV), Cucumovirus, eg Cucumber Mosaic virus (CMV), Comovirus genus such as radish mosaic virus (RaMV), Fabavirus genus such as broad bean virus (BBWV), Nepovirus genus such as tomato ringspot virus ( TomRSV), Sadwavirus genus such as Wenzhou dwarf virus (SDV), Carmovirus genus such as Ron Necrotic spot virus (MNSV), Ophiovirus genus such as Tulip microscopic mosaic virus (TMMMV), Tenuivirus genus such as rice stripe virus (RSV), Tospovirus genus For example, tomato yellow wilt virus (TSWV), phytoreovirus genus such as rice dwarf virus (RDV), begomovirus genus such as tomato yellow leaf curl virus (TYLCV), kaulimovirus Name genera such as cauliflower mosaic virus (CaMV), Pospiviroid, such as chrysanthemum dwarf viroid (CSVd) Possible, the present invention is not limited to these examples.
Among the above pathogenic bacteria, in particular, Helicobacterium, Rhizoctonia, Skrerotium, Homopsis, Pyrenoceta, Choletotricum, Fusarium, Verticillium, Caronectinia, Gomanomyces spp., Roselinia spp., Monosporacus spp., Didimela spp., Sclerotinia spp., Rhizopus spp., Phytophthra spp., Aphanomyces spp., Plasmodiophora spp. Genus, Clavibacter, Pectobacterium, Rhizobium, Ralstonia, Frovirus, Tobamovirus, Tobravirus, Vimovirus, Nepovirus, Carmovirus, Offiovirus, etc. Suitable for soil-borne pathogens according to the present invention It can control.
バエ(Delia platura)、ヒメイエバエ科のヒメイエバエ(Fannia canicularis)、イエバエ科のイエバエ(Musca domestica)、サシバエ(Stomoxys calcitrans)、ニクバエ科のセンチニクバエ(Sarcophaga peregrina)、ウマバエ科のウマバエ(Gasterophilus intestinalis)、ウシバエ科のウシバエ(Hypoderma lineatum)、ヒツジバエ科のヒツジバエ(Oestrus ovis)、チョウ目害虫、例えばコウモリガ科のコウモリガ(Endoclita excrescens)、ツヤコガ科のブドウツヤコガ(Antispila ampelopsia)、ボクトウガ科のゴマフボクトウ(Zeuzera leuconotum)、ハマキガ科のミダレカクモンハマキ(Archips fuscocupreanus)、リンゴコカクモンハマキ(Adoxophyes orana fasciata)、ナシヒメシンクイ(Grapholita molesta)、チャハマキ(Homona magnanima)、マメシンクイガ(Leguminivora glycinivorella)、コドリンガ(Cydia pomonella)、ホソハマキ科のブドウホソハマキ(Eupoecilia ambiguella)、ミノガ科のクロツヤミノガ(Bambalina sp.)、チャミノガ(Eumeta minuscula)、ヒロズコガ科のコクガ(Nemapogon granella)、イガ(Tinea translucens)、チビガ科のナシチビガ(Bucculatrix pyrivorella)、ハモグリガ科のモモハモグリガ(Lyonetia clerkella)、ホソガ科のチャノホソガ(Caloptilia theivora)、キンモンホソガ(Phyllonorycter ringoniella)、コハモグリガ科のミカンハモグリガ(Phyllocnistis citrella)、アトヒゲコガ科のネギコガ(Acrolepiopsis sapporensis)、コナガ科のコナガ(Plutella xylostella)、スガ科のリンゴスガ(Yponomeuta orientalis)、メムシガ科のリンゴヒメシンクイ(Argyresthia conjugella)、スカシバガ科のブドウスカシバ(Nokona regalis)、キバガ科のジャガイモガ(Phthorimaea operculella)、バクガ(Sitotroga cerealella)、ワタアカミムシガ(Pectinophora gossypiella)、シンクイガ科のモモシンクイガ(Carposina sasakii)、マダラガ科のリンゴハマキクロバ(Illiberis pruni)、イラガ科のイラガ(Monema flavescens)、ツトガ科のツトガ(Ancylolomia japonica)、ニカメイガ(Chilo suppressalis)、コブノメイガ(Cnaphalocrocis medinalis)、アワノメイガ(Ostrinia furnacalis)、ヨーロピアンコーンボーラー(Ostrinia nubilalis)、メイガ科のスジマダラメイガ(Cadra cautella)、ハチノスツヅリガ(Galleria mellonella)、トリバガ科のブドウトリバ(Nippoptilia vitis)、アゲハチョウ科のナミアゲハ(Papilio xuthus)、シロチョウ科のモンシロチョウ(Pieris rapae)、セセリチョウ科のイチモンジセセリ(Parnara guttata)、シャクガ科のヨモギエダシャク(Ascotis selenaria)、カレハガ科のマツカレハ(Dendrolimus spectabilis)、オビカレハ(Malacosoma neustrium testaceum)、スズメガ科のエビガラスズメ(Agrius convolvuli)、ドクガ科のチャドクガ(Arna pseudoconspersa)、マイマイガ(Lymantria dispar)、ヒトリガ科のアメリカシロヒトリ(Hyphantria cunea)、ヤガ科のタマナヤガ(Agrotis ipsilon)、タマナギンウワバ(Autographa nigrisigna)、オオタバコガ(Helicoverpa armigera)、コーンイヤーワーム(Helicoverpa zea)、タバコバドワーム(Heliothis virescens)、シロイチモジヨトウ(Spodoptera exigua)、ハスモンヨトウ(Spodoptera litura)、ハチ目害虫、例えばミフシハバチ科のチュウレンジハバチ(Arge pagana)、ハバチ科のクリハバチ(Apethymus kuri)、カブラハバチ(Athalia rosae ruficornis)、タマバチ科のクリタマバチ(Dryocosmus kuriphilus)、スズメバチ科のキイロスズメバチ(Vespa simillima xanthoptera)、アリ科のヒアリ(Solenopsis invicta)、ハキリバチ科のバラハキリバチ(Megachile nipponica)、トビムシ目害虫、例えばマルトビムシ科のキボシマルトビムシ(Bourletiella hortensis)、シミ目害虫、例えばシミ科のセイヨウシミ(Lepisma saccharina)、ヤマトシミ(Ctenolepisma villosa)、ゴキブリ目害虫、例えばゴキブリ科のワモンゴキブリ(Periplaneta americana)、チャバネゴキブリ科のチャバネゴキブリ(Blattella germanica)、シロアリ目害虫、例えばレイビシロアリ科のアメリカカンザイシロアリ(Incisitermes minor)、ミゾガラシロアリ科のイエシロアリ(Coptotermes formosanus)、シロアリ科のタイワンシロアリ(Odontotermes formosanus)、チャタテムシ目害虫、例えばコチャタテ科のコチャタテ(Trogium pulsatorium)、コナチャタテ科のウスグロチャタテ(Liposcelis corrodens)、ハジラミ目害虫、例えばトリハジラミ科のニワトリナガハジラミ(Lipeurus caponis)、ケモノハジラミ科のウシハジラミ(Damalinia bovis)、シラミ目害虫、例えばケモノジラミ科のブタジラミ(Haematopinus suis)、ヒトジラミ科のヒトジラミ(Pediculus humanus)、ケモノホソジラミ科のイヌジラミ(Linognathus setosus)、ケジラミ科のケジラミ(Pthirus pubis)、植物寄生性ダニ類、例えばハシリダニ科のムギダニ(Penthaleus major)、ホコリダニ科のシクラメンホコリダニ(Phytonemus pallidus)、チャノホコリダニ(Polyphagotarsonemus latus)、シラミダニ科のシラミダニの一種(Siteroptes sp.)、ヒメハダニ科のブドウヒメハダニ(Brevipalpus lewisi)、ケナガハダニ科のナミケナガハダニ(Tuckerella pavoniformis)、ハダニ科のアンズアケハダニ(Eotetranychus boreus)、ミカンハダニ(Panonychus citri)、リンゴハダニ(Panonychus ulmi)、ナミハダニ(Tetranychus urticae)、カンザワハダニ(Tetranychus kanzawai)、ナガクダフシダニ科のマツフシダニ(Trisetacus pini)、フシダニ科のミカンサビダニ(Aculops pelekassi)、ナシサビダニ(Epitrimerus pyri)、シトラスラストマイト(Phyllocoptruta oleivora)、ハリナガフシダニ科のイヌツゲフシダニ(Diptacus crenatae)、コナダニ科のムギコナダニ(Aleuroglyphus ovatus)、ケナガコナダニ(Tyrophagus putrescentiae)、ロビンネダニ(Rhizoglyphus robini)を挙げることができるが、本発明はこれらの例に限定されるものではない。上記害虫のうち、特に、アザミウマ目害虫、コウチュウ目害虫、ハエ目、チョウ目などの土壌害虫を本発明により好適に防除できる。 In addition, as pests that can be controlled by the agrochemical formulation composition of the present invention, specifically, grasshopper pests such as the grasshopper (Rusporia lineosa), the cricket family Telegrillus emma, and the vignetting family (Gryllotalpa orientalis), Kobaneinago of acrididae (Oxya yezoensis), migratory locust (Locusta migratoria), My Guratori over grasshoppers (Melanoplus sanguinipes), piggyback grasshopper (Atractomorpha lata) of the piggyback grasshopper family, of pine cricket family Kayakoorogi (Euscyrtus japonicus), of Nomibatta Department Flea grasshopper (Xya japonicus), thrips Eye pests, for example of the thrips family Hirazuhanaazamiuma (Frankliniella intonsa), western flower thrips (Frankliniella occidentalis), yellow tea thrips (Scirtothrips dorsalis), southern thrips (Thrips palmi), green onion thrips (Thrips tabaci), of Kedah thrips family Kakikudaazamiuma ( Ponticulotrips diospyrosi), Haplotrips aculeatus, stink bug pests, for example, Mogania minuta, Aphingidae, Tobiirotsunozemi (Machaerotypus sibiricus), lid Ten leafhopper (Arboridia apicalis) of leafhoppers family, tea Roh green leafhopper (Empoasca onukii), green rice leafhopper (Nephotettix cincticeps), Inazuma leafhopper (Recilia dorsalis), of Hishiunka family Hishiunka (Pentastiridius apicalis), planthoppers Lalodelphax straellatus, Niloparava lugens, White-footed planthopper (Sogataella furcifera), Nymphaeoptera (Nsia nervosa), Red-tailed planthopper (Nsia nervosa) amendaka saccharivora), Red fan gas back (Achilus flammeus of cold wintry wind planthoppers family), tortoiseshell robe (Orosanga japonicus of Hagoromo family), Tobi Gray Ui (Mimophantia maritima of Aobahagoromo family), Nashikijirami of psyllid family (Cacopsylla pyrisuga), Diaphorina citri (Diaphorina citri), Manpidae (Calophya mangiferae), Grape aphid (Daktolosaira vitifoliae), Larixa aphididae (Adelges larvae) tsugae), aphid pea aphids (Acythosiphon pisum), cotton aphids (Aphis gossypis), Aphis spiraecisma, aphid worms ), Wheat beetle (Rhopalosiphum padi), Whitefly of the whitefly family (Aleurocanthus camelliae), Akacanthus spiniferus (Aleurocanthus spiniferus), Tobacco whitefly emisia argentifolii), greenhouse whitefly (Trialeurodes vaporariorum), cotton butterbur scale insect family of giant straw sandals scale insects (Drosicha corpulenta), Iseria scale insects (Icerya purchasi), pineapple mealybug of mealybugs family (Dysmicoccus brevipes), mandarin orange mealybug (Planococcus citri), Pseudococcus comstock, Pseudococcus hornworm (Ceroplastes ceriferus), Pseudococcidae (Acerda Takahashii), Mulculaidae Aonidiella aurantii, Nasimala scale (Diaspidiotus perniciosus), Aphididae (Tropicus), Ternidumarisu, Auriciaceae Azalea (Stephanitis pyrioides), Nasigunbai (Stephanitis nashi), Stink bugs (Eysarcoris aeneus), Rice worms (Laginotomus elongatis) ), Chabae beetle (Plautia crosssota), M. beetle (Megacopta cribraria), M. pteridae (Cavererus moth), Stink bugs (Dysdercus cingulatus), Leptocorisa actina, Leptocorisa chinensis, Lepidoptera helicidae Rhopalus maculatus), bedbug (Cimex lectularis of bed bug family), Coleoptera, for example Scarabaeidae of cupreous chafer (Anomara cuprea), rufocuprea (Anomara rufocuprea), Japanese beetle (Popillia japonica), rhinoceros beetle (Oryctes rhinoceros), Komemushi Department of Tobi Agrootes ogurae, Melanus okinawensi, Melanotus fortumi, Aphididae, Heterobostrychus hamatipennis), Ginkgobill beetle (Stegobium panicum) of the family Asteridae, Nectaraceae (Petinus clavipes), Cocnues of the genus Agrophyceae Carpophilus hempterus, Carabopheles beetle (Ahasverus avena), Carabineidae (Cryptoles ferrugineus) genus v. stis), the beetle, Epilachna vigintioctopunctata (Henosepilachna vigintioctopunctata), Chai Loco Meno mealworm (Tenebrio molitor of Tenebrionidae), red flour beetle (Tribolium castaneum), beans Beetle (Epicauta gorhami of the blister beetle family), gloss of Cerambycid Hada Gomadarakamikiri (Anoplophora glabripennis ), Grape tiger beetle (Xylotrechus pyrrhoderus), pine beetle beetle (Monochamus alternatus), beetle weevil (Callosobchuchus chinensis), potato beetle L. tarsa decemlineata, western corn root worm (Diabrotica virgifera), cucumber moth beetle (Alacophora femorarias), radish beetle (Phaedon brasiumae), physalis weevil (Phylotletolata) Weevil (Hypera postica), Weevil weevil (Listroderes costirostris), Weevil weevil (Euscepes postfascituus), Weevil weevil (Echinocnemus bipunctas), Weevil weevil rhoptrus oryzophylus), weevil of the family weevil (Sitophilus zeamis), shirasoue sushi (Sphenophrus venasis), pine stag beetle (Tomicus pinipacidae), Bark beetle (Lyctus bruneus), fly insect pests, for example, Tipula aino from the family Ganboaceae, Lovebag from the family Fleidae (Plecia neuralcidae) Spring mushroom ( Pnyxia scabiie), soybean flies of the family Frostidae (Asphondylia yushimai), mosquito flies (Aies aegypti), cyprinid moss (Aedes aegypti) (Chironomus oryzae), Chrysops suavis, Tabanas trigonus, Eumerus strigatus, Butterfly, Bactraceae ponica), Mediterranean fruit fly (Ceratitis capitata), leafminer Department of legume leafminer (Liriomyza trifolii), the pea (Chromatomyia horticola), Mugikimoguribae of chloropidae Department (Meromyza nigriventris), cherry fruit fly (Drosophila suzukii of Drosophila family), Drosophila melanogaster (Drosophila melanogaster) , Hydrelia griseola, Hemipodidae (Hippobosca equina), Humperoceae (Parallelpmmasa sasawawee), Onion flies (Delia antiqua), Flies flies (Delia platura), Flies flies flies (Fannia cynariris), Flies flies (Musca domestica), Flies flies (Musca domestica) (Gasterophilus intestinalis), Hypoderma lineatum, Lepidoptera oestrus ovis, Lepidoptera pests, for example, Endocrita sp. sia), Gomafubokutou (Zeuzera leuconotum of Bokutouga family), Mi someone summer fruit moth (Archips fuscocupreanus of Tortricidae), apple Coca summer fruit tortrix (Adoxophyes orana fasciata), oriental fruit moth (Grapholita molesta), Chahamaki (Homona magnanima), soybean pod borer, Leguminivora glycinivorella (Leguminivora glycinivorella ), Cydia pomonella, Eupoecilia abiguella, Brombalina sp. ), Chauminoga (Eumeta minuscula), Nempogon granola, Negatiti vulgaria (Tinea translucens), Nymchigae of the scorpion family (Bucculatrix moth) Golden moth (Phyllononycter lingoniella), Scarlet moth (Phyllocnistis citrella), Scarlet moth (Acroleopsis sapporensis), Stell moth (ell) Yponomeuta orientalis), Argyresthia conjugella, Nokona regalis, Phyramea cereal, Phorimaea opercella, Peach moth (Carposina sasakii), kingfisher Illiberis pruni, moth moth (Monema flavescens), moth (Ancylolomilia japonica sipakiga), s), Cnaphalocrosis medinalisa, African moth (Ostrinia furnacalis), Ni corn borer (Ostrinia nubilalis), Astragalus genus (Citrus moth) Papilio xutus, Pieris rapae, Prunus gnarly, Parnara gutata, Ascotis selenarial, D. Spectabilis, Malacosoma nestrium testaceum, Spruce family shrimp (Agrius convulvali), Arnapseconspersa, Arnapanthiconspersa, Mytah ipsilon, Tamanaginu waiba (Autographa nigrisigna), Helicoverpa armigera, Corn ear worm (Helicoverpa zea), Tobacco budworm (Heliothis virescens d) exigua), Spodoptera litura, bee pests, for example, Arge pagana, Bee family, Aphemus muri, Athaerus Vespa similima xanthoptera, ants of the antaceae (Solenopsis invicta); For example, Lepisma saccharina, Yamatosimi (Ctenolepisma villosa), Cockroachate pests, for example, Cockroachaceae, B. Cannibal termites (Incitermes minor), Cyprids termites (Copttermes formosanus), Termites (Donopterformes formosanus), Pterodoptera, Cicadas Liposcelis corrodens, white-tailed pests, for example, Chrysophyllum dipteridae (Lipeurus caponis), Belgian white-headed lice (Demilinia bovis), L (Pediculus humanus), Canine lice (Linognathus setosus), Phyrus pedis (Pthyrus pubis), plant mites (Penthalus majoli) ( olyphagotarsonemus latus), a kind of Shiramidani of Shiramidani Department (Siteroptes sp. ), Grapes Hime spider mites (Brevipalpus lewisi of Himehadani family), Namikenagahadani (Tuckerella pavoniformis of Kenagahadani family), Anzuakehadani spider mites family (Eotetranychus boreus), citrus red mite (Panonychus citri), Ringohadani (Panonychus ulmi), two-spotted spider mite (Tetranychus urticae), kanzawai (Tetranychus kanzawai), Triticus pini, Scarlet mite (Aculops pelekassi), Pepperid mite (Epitrimerus pylori), Citrus rastroite levora), Dipactus crenatae, Dendracus urticae (Aleuroglyhus ovatus), Phytoid mite (Tyrophagus putrescentiae), Rozne mite (invention) It is not a thing. Among the above-mentioned pests, in particular, soil pests such as thrips pests, Coleoptera pests, flies and butterflies can be suitably controlled according to the present invention.
、ヒメミソハギ(Ammannia multiflora)、ミゾハコベ科雑草、例えばミゾハコベ(Elatine triandra)、コナギ(Monochoria vaginalis)、ミズアオイ(Monochoria korsakowii)、アメリカコナギ(Heteranthera limosa)、オモダカ科雑草、例えばウリカワ(Sagittaria pygmaea)、オモダカ(Sagittaria trifolia)、ヘラオモダカ(Alisma canaliculatum)、アキナシ(Sagittaria aginashi)、ヒルムシロ科雑草、例えばヒルムシロ(Potamogeton distinctus)、ホシクサ科雑草、例えばホシクサ(Eriocaulon cinereum)、セリ科雑草、例えばセリ(Oenanthe javanica)などを挙げることができるが、本発明はこれらの例に限定されるものではない。 Examples of the weeds that can be controlled by the agrochemical formulation composition of the present invention include red cloveraceae weeds such as Oenothera erythrosepala, Oenothera laciniata, buttercup weeds such as Togenino fox ranunculus Ranunculus sardous, terrestrial weeds such as buckwheat (Polygonum convolvus), sanaetadegi (Polygonum raspiforum), Polygonum sap (um) Shi (Rumex obtusifolius), Japanese knotweed (Poligonum cuspidatum), Pennsylvania smart weed (Polygonum pensylvanicum), knotweed (Persicaria longiseta), Ooinutade (Persicaria lapathifolia), Tanisoba (persicaria nepalensis), Portulacaceae weeds such purslane (Portulaca oleracea), dianthus Family weeds such as chickweed (Stellaria media), Dutch tiger rush (Cerastium glomeratum), Stellaria alsine, large clover (Spergula arvensis), cow chickweed (Stell) laria aquatica), red crustaceae weeds such as white cherry (Chenopodium album), broomweed (Kochia scoparaia), red crow (Chepopodium album), red crow (Chepopodium album), red crow hybridus, Amaranthus palmeri, Amaranthus spinosus, Amaranthus rubis, Amaranthus albus, Athus amur s lividus), cruciferous weeds, such as wild radish (Raphanus raphanistrum), black pepper (Sinapis arvensis), cappella bursula rasinus (Lepidium vines), (Rorippa indica), Red-headed burdock (Rorippa islandica), Oyster firefly (Sisymnrium officinale), Red-footed hornet (Cardhamine flexuraosa), Naturturium officina emorosa), legume weeds, such as Sesbania exaltata, Cassia obtusinfolia, Desmodium totuosum, Desmodium totuosum, ), Vulgaria pea (Vicia hirsuta), Yakusou (Kumerowia straitata), Eurasian coconut (Medicago polymorpha), Carrion pea (Vicia angustifolia), Kusanane (Aeschinidiae, Aeschinidae) long theophrasti, Sida spinosa, violet family weeds such as field pansies (Viola arvensis), wild pansies (Viola tricolor), weeping weeds (eg Galium aparinaceae) Ipomoea hederacea), Ipomoea purpurea (Ipomoea purpurea), tall American morning glory (Ipomoea hederacea var integriuscula), beans morning glory (Ipomoea lacunosa), field bindweed (convolvulus arvensis), Noasagao (Ipomoea indica), Ipomoea coccinea (Ipomoea c ccinea, Ipomoea triloba, Lamiaceae weeds, such as Lamium puruumum, Lamum ampurisau, L. Physalis angula (Physalis angulata), American dogwood (Solanum americanum), Barnacle beetle (Solanum carolinense), scorpionaceae weeds, for example, Veronica persica (Vernica persica), Sabasou (Veronica hederaefolia, Asteraceae weeds, for example Eclipta prostrata (Eclipta prostrata), Bidens Tripartita (Bidens tripartita), cocklebur (Xanthium pensylvanicum), wild sunflower (Helianthus annuus), chamomile (Matricaria chamomilla), Inukamitsure (Matricaria perforataorinodora), corn marigold (Chrysanthhemum segetum), Koshikagiku (Matricaria matricarioides), Ragweed (Ambrosia artemisifolia), Obraxa trifida (Ambrosia trifida) igeron canadensis), mugwort (Artemisia princeps), goldenrod (Solidago altissima), dandelion (Taraxacum officinale), chamomile beetle (Anthemis cotula), Ezo Roh fox thistle (Breea setosa), Carduus (Sonchus oleraceus), Jerusalem artichoke (Helianthus tuberosus), Black thistle (Cirsium arvense), Bidens frontosa (Bidens pilosa), cornflower (Centurea cyanus), American thistle (Cirsium vulgares) L tuca scariola, Rudbeckia hirta, Greater Redhorn, Rudbeckia lacinia, Rudbeckia lacinia var. hortensis Bailey), Senecio vulgaris (Senecio vulgais), milk thistle (Silybum marianum), Sonchus Asper (Sonchus asper), field sow thistle (Sonchus arvensis), Hosobaokahijiki (Salsola kali), Naruto ragwort (Senecio madagascariensis), Coreopsis lanceolata (Coreopsis lanceolata) Purple weeds, for example Myosotis arvensis, moth family weeds, for example Asclepias syriaca, Euphorbiaceae weeds, for example Euphorbia helioscopia lata), Enokirusha (Acalypha australis), Aspergillus weeds, for example, American spider (Geranium carolinianum), Oxalis weeds, such as Oxalis corymbosa, Ulridae, S. (Echinochloa oryzicola), Azegaya (Leptochloa chinensis), Chigosasa (Isachne globosa), Paspalum distichoza (Lessia saisau) -Galli), Enocorosa (Setaria viridis), Aquinoenokorogusa (Setaria Faberi), Eurasian burrows (Digitaria esugalis), Eurasiania urus (European urus) Sorghum haremense, Agropyron repens, Bromus tectorum, Cynone dactylum, Panicum dichotomiflorum Texas Ken (Sorghum vulgare), foxtail (Alopecurus geniculatus), darnel (Lolium multiflorum), rigid ryegrass (Lolium rigidum), Setaria pumila (Setaria glauca), beckmannia syzigachne (Beckmannia syzigachne), commelinaceae weeds, for example dayflower (Commelina communis), Ibokusa ( Murdannia keisak), Toxaceae weeds such as Equisetum arvense, Poppy weeds such as Papaver rhoeas, Cyperaceae weeds such as Cyperus iriat Masuge (Cyperus esculentus), smallflower umbrellaplant (Cyperus difformis), bulrush (Scirpus hotarui), Eleocharis acicularis (Eleocharis acicularis), Cyperus (Cyperus serotinus), water chestnut (Eleocharis kuroguwai), fimbristylis miliacea (Fimbristylis miliacea), Hinagayatsuri (Cyperus flaccidus), Azegayatsuri ( Cyperus globosus, Sirpus juncoides, Shirapus wallicii, Sirpus nipponicus, Fenbritsalis autumnalis (Scirpus tabernaemontani), Scrophulariaceae weeds, for example, false pimpernel (Lindernia procumbens), Amerikaazena (Lindernia dubia), Abunome (Dopatrium junceum), Ooabunome (Gratiola japonica), Azetougarashi (Lindernia angustifolia), LIMNOPHILA SESSILIFLORA (Limnophila sessiliflora), Lythraceae weeds, For example, Rica indica
, Ammannia multiflora Roxb (Ammannia multiflora), elatinaceae weeds, for example Elatine triandra Schk (Elatine triandra), Monochoria (Monochoria vaginalis), Monochoria korsakowii (Monochoria korsakowii), the United States vaginalis (Heteranthera limosa), Alismataceae weeds, for example arrowhead (Sagittaria pygmaea), Alismataceae ( Sagittaria trifolia, Alasma canalicatum, Akinashi (Sagittaria aginashi), Weevil weeds, such as Potamogeton distinctus, E. um), Umbelliferae weeds such Seri (Oenanthe javanica) and the like can be mentioned, the present invention is not limited to these examples.
無機塩培地(0.2質量%NaNO3、0.1質量%KH2PO4、0.02質量%KCl、0.02質量%MgSO4・7H2O)に酢酸を所定濃度添加したものを供試培地とした。トリコデルマ・アスペレロイデス SKT-1株、トリコデルマ・ハルジアナム T-22株、及びトリコデルマ・ハマタム NBRC 31932株の各糸状菌について、胞子懸濁液(1×106胞子/ml)0.5mlを、供試培地50mlを含む100ml容三角フラスコに植菌し、振とう培養(27℃、120rpm、3日間)した。培養後、各菌株の菌糸生育状況を達観調査した。これらの試験結果を表1に示した。
また、同様の試験をタラロマイセス・フラバス(flavus) SAY-Y-94-01株及びメタリジウム・アニソプリエ(anisopliae) SMZ-2000株についても行った。
なお、トリコデルマ・アスペレロイデス SKT-1株は、エコホープ(登録商標、クミアイ化学工業株式会社製)の商品名で農薬として販売されている。トリコデルマ・ハルジアナム T-22株は、トリコデソイル(登録商標、アリスタライフサイエンス株式会社製)の商品名で土壌改良材として販売されている。トリコデルマ・ハマタム NBRC 31932株は、独立行政法人製品評価技術基盤機構より分譲された。タラロマイセス・フラバス SAY-Y-94-01株は、タフブロック(登録商標、出光興産株式会社製)の商品名で農薬として販売されている。メタリジウム・アニソプリエ SMZ-2000株は、パイレーツ粒剤(登録商標、アリスタライフサイエンス株式会社製)の商品名で農薬として販売されている。 (Test of the effect of promoting the growth of mycelium by acetic acid in various fungal spores)
Mineral salts medium which acetic acid was predetermined concentration added (0.2 wt% NaNO 3, 0.1 wt% KH 2 PO 4, 0.02 wt% KCl, 0.02 wt% MgSO 4 · 7H 2 O) A test medium was used. For each filamentous fungus of Trichoderma aspereloides SKT-1, S. Trichoderma harzianum T-22, and Trichoderma hamatum NBRC 31932, 0.5 ml of spore suspension (1 × 10 6 spores / ml) was added to the test medium. A 100 ml Erlenmeyer flask containing 50 ml was inoculated and cultured with shaking (27 ° C., 120 rpm, 3 days). After the culture, the mycelial growth of each strain was objectively investigated. The test results are shown in Table 1.
Similar tests were also performed on the Talalomyces flavus SAY-Y-94-01 strain and the metalidium anisoprie SMZ-2000 strain.
The Trichoderma aspereloides SKT-1 strain is sold as an agrochemical under the trade name Ecohope (registered trademark, manufactured by Kumiai Chemical Industry Co., Ltd.). Trichoderma harzianum T-22 strain is sold as a soil conditioner under the trade name of Trichodesoil (registered trademark, manufactured by Arista Life Science Co., Ltd.). Trichoderma hamatum NBRC 31932 shares were sold by National Institute of Technology and Evaluation. Talalomyces flavus SAY-Y-94-01 is marketed as an agrochemical under the trade name Tough Block (registered trademark, manufactured by Idemitsu Kosan Co., Ltd.). Metalidium anisoprie SMZ-2000 strain is marketed as an agrochemical under the trade name of Pirate Granules (registered trademark, manufactured by Arista Life Science Co., Ltd.).
酢酸を配合した農薬製剤組成物として、本発明品として酢酸原末被覆製剤及び酢酸直噴製剤、比較品としてSKT-1株液剤(商品名:「エコホープ」、クミアイ化学工業株式会社製)に酢酸を15体積%混用した製剤の3製剤を作製した。酢酸原末被覆製剤は、酢酸をホワイトカーボンに吸着して15質量%の原末 とし、SKT-1株大麦種子培地培養物(大麦種子(品種:ハヤドリ2)8g、YG培地(0.3質量%酵母エキス、2.0質量%グルコース)8mlを含む100ml容三角フラスコにSKT-1株胞子懸濁液(1×106胞子/ml)0.5mlを植菌し、25℃で8日間静置培養した後、一晩自然風乾させたもの。以下、SKT-1株固体培養物ともいう):前記原末=99:1(質量比)の割合で、ポリ袋内でよく混合することにより被覆した。酢酸直噴製剤は、酢酸を水に希釈して15質量%の溶液 とし、SKT-1株固体培養物:前記溶液=99:1(質量比)の割合で、ポリ袋内でSKT-1株固体培養物を振り混ぜながら前記溶液を噴霧することにより酢酸を吸着した。作製後、製剤をアルミ袋包装し、5℃で保管し、0日後(初期値)、1日後及び7日後、希釈平板法により製剤中生菌数を測定した。この試験結果を表2に示した。 (Storage stability test of agrochemical formulation)
As an agrochemical preparation composition containing acetic acid, acetic acid bulk powder coating preparation and acetic acid direct injection preparation as a product of the present invention, and SKT-1 stock solution (trade name: “Eco-Hope”, manufactured by Kumiai Chemical Industry Co., Ltd.) as acetic acid 3 preparations of 15% by volume were prepared. The acetic acid bulk powder coating preparation was prepared by adsorbing acetic acid to white carbon to give 15% by weight of bulk powder, 8 g of SKT-1 strain barley seed medium culture (barley seed (variety: Hayadri 2)), YG medium (0.3 mass) 0.5 ml of SKT-1 strain spore suspension (1 × 10 6 spores / ml) was inoculated into a 100 ml Erlenmeyer flask containing 8 ml of 2% yeast extract and 2.0% by weight glucose, and allowed to stand at 25 ° C. for 8 days. After standing and then air-dried overnight (hereinafter also referred to as SKT-1 strain solid culture): by mixing well in a plastic bag at a ratio of the above bulk powder = 99: 1 (mass ratio) Covered. The acetic acid direct-injection preparation was prepared by diluting acetic acid in water to give a 15% by mass solution, and the SKT-1 strain solid culture: the above-mentioned solution = 99: 1 (mass ratio) in a SKT-1 strain in a plastic bag. Acetic acid was adsorbed by spraying the solution while shaking the solid culture. After preparation, the preparation was packed in an aluminum bag and stored at 5 ° C. After 0 days (initial value), 1 day and 7 days later, the viable cell count in the preparation was measured by a dilution plate method. The test results are shown in Table 2.
キュウリ白絹病菌(Sclerotium rolfsii)をPDA平板培地で前培養後、含菌寒天片を土壌ふすま培地(砂壌土2.5kg、ふすま2.0kg、酵母エキス3g、グルコース20g、蒸留水1L)に混和し、27℃で7日間培養した。この接種源と土壌(滅菌砂壌土:園芸培土(商品名:「くみあいニッピ園芸培土1号」、日本肥糧株式会社製)=1:1(体積比))を1:5(体積比)の割合でよく混和し、湿室(27℃、暗所)に一晩放置したものを汚染土壌とした。この汚染土壌約15mlをプラスチックカップ(径6cm)で育苗した子葉期キュウリ(品種:日支)の株元に敷き詰めることで接種した。接種後、実施例2の酢酸原末被覆製剤及び酢酸直噴製剤とSKT-1株固体培養物はキュウリの株元に500g/m2相当を散布し、SKT-1株液剤はSKT-1株液剤:水=1:14(質量比)の割合で希釈した散布液をキュウリの株元に3L/m2相当を灌注し、トルクロホスメチル水和剤(商品名:「リゾレックス水和剤」(登録商標)、住友化学株式会社製)はトルクロホスメチル水和剤:水=1:999(質量比)の割合で希釈した散布液をキュウリの株元に3L/m2相当を灌注した。薬剤処理後、湿室(27℃、暗所)内で3日間管理した後、発病の有無を調査し、以下の計算式により防除価を算出した。この試験結果を表3に示した。 (Cucumber white silkworm disease control effect test of agricultural chemical formulation composition)
After pre-culturing cucumber white silkworm (Sclerotium rolfsii) in PDA plate medium, the bacterial agar pieces are mixed with soil bran medium (2.5 kg sand loam, 2.0 kg bran, 3 g yeast extract, 20 g glucose, 1 L distilled water). And cultured at 27 ° C. for 7 days. 1: 5 (volume ratio) of this inoculation source and soil (sterilized sand loam soil: horticultural culture soil (trade name: “Kumiai Nippi Horticulture Culture No. 1”, manufactured by Nippon Fertilizer Co., Ltd.) = 1: 1 (volume ratio)) Contaminated soil was obtained by mixing well at a ratio and leaving it in a moist chamber (27 ° C., dark place) overnight. About 15 ml of this contaminated soil was inoculated by spreading on the stock of a cotyledon stage cucumber (variety: Nissho) that was raised in a plastic cup (diameter 6 cm). After inoculation, the acetic acid bulk powder coating preparation and direct acetic acid injection preparation of Example 2 and SKT-1 strain solid culture were sprayed at 500 g / m 2 equivalent to the cucumber stock, and the SKT-1 stock solution was the SKT-1 strain. Liquid: Water = 1:14 (mass ratio) diluted spray solution is irrigated with 3 L / m 2 equivalent to the cucumber stock, Torcrolofos methyl wettable powder (trade name: “Risolex wettable powder” (Registered) Trademark), manufactured by Sumitomo Chemical Co., Ltd., irrigated 3 L / m 2 of cucumber stock with a spray solution diluted at a ratio of torquelophosmethyl wettable powder: water = 1: 999 (mass ratio). After the treatment with the chemical, it was managed in a moist chamber (27 ° C., dark place) for 3 days, and then the presence or absence of disease was investigated, and the control value was calculated by the following formula. The test results are shown in Table 3.
実施例3と同様の方法により、酢酸原末被覆製剤のキュウリ白絹病防除効果試験を実施した。なお、酢酸原末被覆製剤散布量は実施例3の倍量で実施した。この試験結果を実施例3のデータと比較したものを表4に示した。 (Cucumber white silkworm disease control effect test by the difference in the processing amount of the agricultural chemical formulation composition)
By the method similar to Example 3, the cucumber silkworm disease control effect test of the acetic acid bulk powder coating preparation was implemented. In addition, the acetic acid bulk powder coating preparation application amount was carried out in double the amount of Example 3. Table 4 shows a comparison of the test results with the data of Example 3.
酢酸をホワイトカーボンに吸着して50質量%、15質量%又は5質量%の原末とし、SKT-1株固体培養物:前記原末=99:1(質量比)の割合で、ポリ袋内でよく混合することにより被覆し、酢酸配合量が0.5質量%、0.15質量%又は0.05質量%の酢酸原末被覆製剤をそれぞれ作製した。4℃で1週間保管後、製剤中生菌数及びキュウリ白絹病防除効果を調査した。実施例2と同様の方法により製剤中生菌数を、実施例3と同様の方法によりキュウリ白絹病防除効果を調査した。製剤中生菌数の試験結果を表5、キュウリ白絹病防除効果の試験結果を表6に示した。 (Storage stability due to the difference in the amount of acetic acid in the agricultural chemical formulation, cucumber white silkworm prevention)
Acetic acid is adsorbed on white carbon to make 50%, 15% or 5% by mass of the bulk powder, and the SKT-1 strain solid culture: the bulk powder = 99: 1 (mass ratio) Were mixed well to prepare acetic acid bulk powder coating preparations having an acetic acid content of 0.5% by mass, 0.15% by mass or 0.05% by mass, respectively. After storage at 4 ° C. for 1 week, the number of viable bacteria in the preparation and the cucumber silkworm disease control effect were investigated. The number of viable bacteria in the preparation was examined by the same method as in Example 2, and the cucumber silkworm disease control effect was examined by the same method as in Example 3. Table 5 shows the test results of the number of viable bacteria in the preparation, and Table 6 shows the test results of the cucumber silkworm disease control effect.
キュウリ苗立枯病菌(Rhizoctonia solani AG-4)をPDA平板培地で前培養後、含菌寒天片を土壌ふすま培地に混和し、27℃で7日間培養した。この接種源と土壌(滅菌砂壌土:園芸培土=1:1(体積比))を1:50(体積比)の割合でよく混和し、湿室(27℃、暗所)に一晩放置したものを汚染土壌とした。角鉢(9cm四方)に滅菌川砂200ml、汚染土壌100mlを順に充填した。なお、無接種区は、汚染土壌の代わりに、土壌ふすま培地:土壌=1:50(体積比)で混和したもの100mlを充填した。実施例2の酢酸原末被覆製剤(酢酸配合量0.15%)の土壌混和は、汚染土壌を角鉢へ充填前、汚染土壌100mlと酢酸原末被覆製剤8.1g(1000g/m2相当)をポリ袋内で混和し、角鉢に充填、灌水した後、キュウリ(品種:日支)を播種した。酢酸原末被覆製剤の表層散布は、灌水後、キュウリ種子を汚染土壌に播種後、500g/m2相当を土壌表層に均一に散布した。比較として、トルクロホスメチル水和剤:水=1:499(質量比)の割合で希釈した散布液3L/m2相当を播種前に土壌灌注処理した。薬剤処理後は、湿室(27℃、暗所)内で一晩静置後、温室内で管理した(頭上灌水)。接種8日後、以下の発病指数の基準にしたがって指数調査し、また、以下の数式により発病度、補正発病度及び防除価を算出した。この試験結果を表7に示した。 (Control effect of cucumber seedling blight (Rhizoctonia spp.) Of agricultural chemical formulation composition)
Cucumber seedling blight fungus (Rhizotonia solani AG-4) was pre-cultured on a PDA plate medium, and the agar-containing agar pieces were mixed with soil bran medium and cultured at 27 ° C. for 7 days. This inoculation source and soil (sterilized sand loam soil: horticultural soil = 1: 1 (volume ratio)) were mixed well at a ratio of 1:50 (volume ratio) and left overnight in a humid room (27 ° C., dark place). The soil was contaminated soil. A square bowl (9 cm square) was filled with 200 ml of sterile river sand and 100 ml of contaminated soil in this order. In addition, the non-inoculated section was filled with 100 ml of a mixture of soil bran medium: soil = 1: 50 (volume ratio) instead of contaminated soil. Soil incorporation of acetate bulk powder coating formulation of Example 2 (acetate amount 0.15%) is filled before, contaminated soil 100ml and acetic bulk powder coating formulation 8.1 g (1000 g / m 2 corresponds to the contaminated soil to the corner pots ) Was mixed in a plastic bag, filled into a square bowl, watered, and then sown with cucumber (variety: Nissho). The surface layer spraying of the acetic acid bulk powder coating preparation was carried out by spraying cucumber seeds on the contaminated soil after irrigation and then uniformly spraying 500 g / m 2 equivalent on the soil surface layer. As a comparison, 3 L / m 2 of spray solution diluted at a ratio of torquelofos methyl wettable powder: water = 1: 499 (mass ratio) was subjected to soil irrigation treatment before sowing. After the drug treatment, it was allowed to stand overnight in a wet room (27 ° C., dark place) and then managed in a greenhouse (overhead irrigation). Eight days after the inoculation, the index was investigated according to the criteria of the following disease index, and the disease severity, corrected disease severity and control value were calculated according to the following mathematical formula. The test results are shown in Table 7.
0:発病なし
1:生育異常
2:枯死又は未出芽 <Disease index>
0: No disease 1: Growth abnormality 2: Withering or unbudding
補正発病度=各区の平均発病度-無接種区の平均発病度
防除価=(1-処理区の補正発病度/無処理区の補正発病度)×100 Disease severity = Σ (number of diseased seedlings by index × index) / (number of surveyed seedlings × 2) × 100
Corrected disease severity = average disease severity in each zone−average disease severity control value in non-inoculated zone = (1−corrected disease severity in treated zone / corrected disease severity in untreated zone) × 100
キュウリ苗立枯病菌(Pythium aphanidermatum)をPDA平板培地で前培養後、含菌寒天片を土壌ふすま培地に混和し、27℃で7日間培養した。この接種源と土壌(滅菌砂壌土:園芸培土=1:1(体積比))を1:300(体積比)の割合でよく混和し、湿室(27℃、暗所)に一晩放置したものを汚染土壌とした。角鉢(9cm四方)に滅菌川砂200ml、汚染土壌100mlを順に充填した。なお、無接種区は、汚染土壌の代わりに、土壌ふすま培地:土壌=1:300(体積比)で混和したもの100mlを充填した。実施例2の酢酸原末被覆製剤(酢酸配合量0.15質量%)の土壌混和は、汚染土壌を角鉢へ充填前、汚染土壌100mlと酢酸原末被覆製剤4.1g(500g/m2相当)をポリ袋内で混和し、角鉢に充填、灌水した後、キュウリ(品種:日支)を播種した。ヒドロキシイソキサゾール液剤(商品名:「タチガレン液剤」(登録商標)、三井化学アグロ株式会社製)はヒドロキシイソキサゾール液剤:水=1:499(体積比)の割合で希釈した散布液3L/m2相当を播種後に土壌灌注処理した。薬剤処理後は、湿室(27℃、暗所)内で一晩静置後、温室内で管理した。接種12日後、以下の発病指数の基準にしたがって指数調査し、また、以下の数式により発病度、補正発病度及び防除価を算出した。この試験結果を表8に示した。 (Effects of cucumber seedling blight (Pisium fungus) control of agrochemical formulation)
A cucumber seedling fungus (Pythium aphanidermatum) was pre-cultured on a PDA plate medium, and the agar-containing agar pieces were mixed with a soil bran medium and cultured at 27 ° C. for 7 days. This inoculation source and soil (sterilized sand loam soil: horticultural soil = 1: 1 (volume ratio)) were mixed well at a ratio of 1: 300 (volume ratio) and left overnight in a wet room (27 ° C., dark place). The soil was contaminated soil. A square bowl (9 cm square) was filled with 200 ml of sterile river sand and 100 ml of contaminated soil in this order. In addition, the non-inoculated section was filled with 100 ml of a mixture of soil bran medium: soil = 1: 300 (volume ratio) instead of contaminated soil. The soil mixing of the acetic acid bulk powder coating formulation of Example 2 (acetic acid content 0.15% by mass) was performed before filling the contaminated soil into a bowl, 100 ml of contaminated soil and 4.1 g of acetic acid bulk powder coating formulation (500 g / m 2). The mixture was mixed in a plastic bag, filled into a square bowl, watered, and then sown with cucumber (variety: Nissho). Hydroxyisoxazole solution (trade name: “Tatigalen solution” (registered trademark), manufactured by Mitsui Chemicals Agro Co., Ltd.) was diluted with a ratio of hydroxyisoxazole solution: water = 1: 499 (volume ratio) 3 L / spray solution and soil drench treatment m 2 corresponds to the after seeding. After the chemical treatment, it was allowed to stand overnight in a wet room (27 ° C., dark place) and then managed in a greenhouse. 12 days after the inoculation, the index was investigated according to the criteria of the following disease index, and the disease severity, the corrected disease severity and the control value were calculated by the following mathematical formulas. The test results are shown in Table 8.
0:発病なし
1:生育異常
2:枯死又は未出芽 <Disease index>
0: No disease 1: Growth abnormality 2: Withering or unbudding
補正発病度=各区の平均発病度-無接種区の平均発病度
防除価=(1-処理区の補正発病度/無処理区の補正発病度)×100 Disease severity = Σ (number of diseased seedlings by index × index) / (number of surveyed seedlings × 2) × 100
Corrected disease severity = average disease severity in each zone−average disease severity control value in non-inoculated zone = (1−corrected disease severity in treated zone / corrected disease severity in untreated zone) × 100
供試穀物として、米種子(品種:キヌヒカリ)、小麦種子(品種:農林61号)、大麦種子(品種:ハヤドリ2)、大豆種子(品種:エンレイ)、米籾殻、小麦糠(ふすま)を用いた。各種穀物8g、YG培地(0.3質量%酵母エキス、2.0質量%グルコース)8mlを含む100ml容三角フラスコにSKT-1株胞子懸濁液(1×106胞子/ml)0.5mlを植菌し、25℃で8日間静置培養した。培養後は、一晩自然風乾させた後、SKT-1株培養物と、酢酸をホワイトカーボンに吸着させ酢酸を15質量%含有した原末を99:1(質量比)の割合で、ポリ袋内でよく混合することにより被覆し、酢酸原末被覆製剤を作製した。これら製剤について、実施例3と同様の方法によりキュウリ白絹病防除効果を調査した。この試験結果を表9に示した。 (Control effect of cucumber white silkworm by the difference of various cereal culture media of agricultural chemical formulation composition)
Rice grains (variety: Kinuhikari), wheat seeds (variety: Norin 61), barley seeds (variety: Hayadori 2), soybean seeds (variety: Enrei), rice husks, wheat bran It was. SKT-1 strain spore suspension (1 × 10 6 spores / ml) 0.5 ml in a 100 ml Erlenmeyer flask containing 8 g of various grains and 8 ml of YG medium (0.3 mass% yeast extract, 2.0 mass% glucose) Was inoculated and statically cultured at 25 ° C. for 8 days. After culturing, after air-drying overnight, a SKT-1 strain culture and a bulk powder containing acetic acid adsorbed on white carbon and containing 15% by mass of acetic acid at a ratio of 99: 1 (mass ratio) The mixture was coated by mixing well to prepare an acetic acid bulk powder coating preparation. About these preparations, the cucumber silkworm disease control effect was investigated by the same method as Example 3. The test results are shown in Table 9.
(1)トリコデルマ・アスペレロイデス(Trichoderma asperelloides) SKT-1株(FERM BP-16510)。 The accession numbers of microorganisms deposited internationally in the present invention are shown below.
(1) Trichoderma aspereloides SKT-1 strain (FERM BP-16510).
Claims (24)
- 穀物の種子、その外皮、糠、精白物から選ばれる1以上を固体培地とした生物農薬糸状菌固体培養物と、酢酸を含有してなる農薬製剤組成物。 A bio-pesticide filamentous fungus solid culture in which at least one selected from cereal seeds, its hulls, straws, and refined products is used as a solid medium, and an agrochemical formulation composition containing acetic acid.
- 生物農薬糸状菌が、病害虫及び/又は雑草に対する防除作用、並びに、植物成長調整作用を有する糸状菌である請求項1に記載の農薬製剤組成物。 The agrochemical formulation composition according to claim 1, wherein the biopesticide filamentous fungus is a filamentous fungus having a controlling action against pests and / or weeds and a plant growth regulating action.
- 病害虫及び/又は雑草に対する防除作用、並びに、植物成長調整作用を有する糸状菌が、ボーベリア(Beauveria)属、コニオチリウム(Coniothyrium)属、メタリジウム(Metarhizium)属、タラロマイセス(Talaromyces)属、トリコデルマ(Trichoderma)属、バーティシリウム(Verticillium)属のいずれかに属する菌である請求項2に記載の農薬製剤組成物。 Filamentous fungi having a controlling action against pests and / or weeds and a plant growth regulating action include the genus Beauveria, the genus Coniothyrium, the genus Metalaridium, the genus Talaromyces, the genus Trichoderma The pesticidal composition according to claim 2, which is a bacterium belonging to any one of the genus Verticillium.
- 病害虫及び/又は雑草に対する防除作用、並びに、植物成長調整作用を有する糸状菌が、トリコデルマ属菌である請求項3に記載の農薬製剤組成物。 The agrochemical formulation composition according to claim 3, wherein the fungus having a controlling action against pests and / or weeds and a plant growth regulating action is Trichoderma spp.
- トリコデルマ属菌が、アスペレロイデス(asperelloides)種菌、アスペレラム(asperellum)種菌、アトロビリデ(atroviride)種菌、ハマタム(hamatum)種菌、ハルジアナム(harzianum)種菌、コニンギ(koningii)種菌のいずれかである請求項4に記載の農薬製剤組成物。 The genus Trichoderma is an inoculum of Aspereloides, an inoculum of Asperellum, an atroviride, an inoculum of hamatum, or an inoculum of Koningii Agrochemical formulation composition.
- トリコデルマ属菌が、アスペレロイデス種菌である請求項5に記載の農薬製剤組成物。 6. The agrochemical formulation composition according to claim 5, wherein the Trichoderma spp. Is Aspereroides sp.
- アスペレロイデス種菌が、トリコデルマ・アスペレロイデス(Trichoderma asperelloides) SKT-1株(FERM BP-16510)である請求項6に記載の農薬製剤組成物。 7. The agrochemical formulation composition according to claim 6, wherein the Aspereroides inoculum is Trichoderma aspereloides SKT-1 strain (FERM BP-16510).
- 農薬製剤組成物中1g中に生物農薬糸状菌が10~1010CFU(Colony Forming Unit)含まれることを特徴とする請求項1~7のいずれか1項に記載の農薬製剤組成物。 The agrochemical formulation composition according to any one of claims 1 to 7, wherein 10 to 10 10 CFU (Colony Forming Unit) of the biopesticide filamentous fungus is contained in 1 g of the agrochemical formulation composition.
- 固体培地が、穀物の種子の精白物である請求項1~8のいずれか1項に記載の農薬製剤組成物。 The agrochemical composition according to any one of claims 1 to 8, wherein the solid medium is a refined product of cereal seeds.
- 穀物が、イネ科穀物である請求項1~9のいずれか1項に記載の農薬製剤組成物。 The agrochemical formulation according to any one of claims 1 to 9, wherein the cereal is a gramineous cereal.
- イネ科穀物が、大麦である請求項10に記載の農薬製剤組成物。 The agricultural chemical formulation composition according to claim 10, wherein the gramineous grain is barley.
- 酢酸の含量が、農薬製剤組成物中0.01~1質量%である請求項1~11のいずれか1項に記載の農薬製剤組成物。 The agricultural chemical formulation composition according to any one of claims 1 to 11, wherein the acetic acid content is 0.01 to 1% by mass in the agricultural chemical formulation composition.
- 粒状農薬製剤組成物であって、固体培地が粒状物であることを特徴とする請求項1~12のいずれか1項に記載の農薬製剤組成物。 13. The agrochemical formulation composition according to any one of claims 1 to 12, which is a granular agrochemical formulation composition, wherein the solid medium is a granular material.
- 酢酸が、鉱物質微粉に酢酸が吸着されてなる酢酸吸着物である請求項13に記載の農薬製剤組成物。 The agricultural chemical formulation composition according to claim 13, wherein the acetic acid is an acetic acid adsorbate obtained by adsorbing acetic acid on a fine mineral powder.
- 鉱物質微粉が、含水非晶質二酸化珪素である請求項14に記載の農薬製剤組成物。 The agrochemical composition according to claim 14, wherein the mineral fine powder is hydrous amorphous silicon dioxide.
- 酢酸吸着物の含量が、農薬製剤組成物中0.5~2質量%である請求項14又は15に記載の農薬製剤組成物。 The agricultural chemical formulation composition according to claim 14 or 15, wherein the content of acetic acid adsorbate is 0.5 to 2% by mass in the agricultural chemical formulation composition.
- 生物農薬糸状菌を、穀物の種子、その外皮、糠、精白物から選ばれる1以上を固体培地として用いて固体培養し、培養した生物農薬糸状菌が固体培地に付着する生物農薬糸状菌固体培養物を得た後、該培養物に酢酸を添加することを特徴とする農薬製剤組成物の製造方法。 Biopesticide filamentous fungi solid culture using one or more selected from cereal seeds, their hulls, persimmons, and refined products as a solid medium, and the cultured biopesticide filamentous fungus adheres to the solid medium A method for producing an agrochemical formulation comprising adding acetic acid to the culture after obtaining the product.
- 農薬製剤組成物が粒状物であって、固体培地が粒状物であることを特徴とする請求項17に記載の方法。 The method according to claim 17, wherein the agricultural chemical preparation composition is a granular material, and the solid medium is a granular material.
- 生物農薬糸状菌固体培養物の表面に、酢酸又は酢酸を水もしくは有機溶媒で希釈した溶液を噴霧することにより酢酸を担持させることを特徴とする請求項17又は18に記載の方法。 The method according to claim 17 or 18, wherein acetic acid is supported on the surface of the biopesticide filamentous fungus solid culture by spraying acetic acid or a solution obtained by diluting acetic acid with water or an organic solvent.
- 酢酸を鉱物質微粉に吸着させ、生物農薬糸状菌固体培養物の表面に該吸着物を被覆することを特徴とする請求項18に記載の方法。 The method according to claim 18, wherein acetic acid is adsorbed on the fine mineral powder, and the adsorbate is coated on the surface of the biopesticide filamentous fungus solid culture.
- 鉱物質微粉が、含水非晶質二酸化珪素である請求項20に記載の方法。 The method according to claim 20, wherein the mineral fine powder is hydrous amorphous silicon dioxide.
- 請求項1~16のいずれか1項に記載の農薬製剤組成物を、植物の苗、苗木又は種子に施用し、該植物を栽培することを特徴とする、雑草、病害、害虫から選ばれる1以上の防除及び/又は植物成長調整方法。 A composition selected from the group consisting of weeds, diseases, and pests, wherein the agrochemical preparation composition according to any one of claims 1 to 16 is applied to plant seedlings, seedlings or seeds, and the plants are cultivated. The above control and / or plant growth adjustment method.
- 請求項1~16のいずれか1項に記載の農薬製剤組成物を、植物を栽培する土壌に施用することを特徴とする、雑草、病害、害虫から選ばれる1以上の防除及び/又は植物成長調整方法。 One or more pest control and / or plant growth selected from weeds, diseases, and pests, characterized in that the agrochemical formulation composition according to any one of claims 1 to 16 is applied to soil for plant cultivation. Adjustment method.
- 土壌1m2あたり農薬製剤組成物を5~1000g施用することを特徴とする請求項23に記載の方法。 The method according to claim 23, wherein 5 to 1000 g of the agricultural chemical preparation composition is applied per 1 m 2 of soil.
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---|---|---|---|---|
CN111631136A (en) * | 2020-07-14 | 2020-09-08 | 安顺职业技术学院 | Tissue culture and rapid propagation method of dormant buds of eyedrops |
CN112646734A (en) * | 2020-12-31 | 2021-04-13 | 广西壮族自治区林业科学研究院 | Orchid mycorrhizal fungus PF06 and application thereof |
Families Citing this family (4)
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WO2020028463A1 (en) * | 2018-08-02 | 2020-02-06 | Drexel University | An urban in-home system for growing fruits and vegetables |
KR102193709B1 (en) | 2019-01-25 | 2020-12-21 | 인바이오 주식회사 | Agricultural chemical composition of water-dispersible granule type and preparation method thereof |
CN113180065B (en) * | 2021-05-27 | 2022-06-24 | 贵州省生物技术研究所(贵州省生物技术重点实验室、贵州省马铃薯研究所、贵州省食品加工研究所) | Application of tartary buckwheat sprout extract |
CN114872157B (en) * | 2022-06-07 | 2023-05-09 | 福建省林业科学研究院 | Method for treating trunk of new dead pine wood nematode epidemic disease wood |
Citations (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5341424A (en) * | 1976-09-13 | 1978-04-14 | Ricard Jacques | Mold killing agent especially for processing plants |
JPH06192028A (en) * | 1992-12-25 | 1994-07-12 | Hokkaido Green Kosan:Kk | Plant blight controlling microorganism, controlling agent production by using the same, production of controlling agent and using method therefor |
JPH06256125A (en) * | 1993-03-09 | 1994-09-13 | Eezai Seikaken Kk | Controlling agent for white root rot |
JPH0987122A (en) * | 1995-09-21 | 1997-03-31 | Hokkaido Green Kosan:Kk | Disease control for plant |
JPH11225745A (en) * | 1997-11-13 | 1999-08-24 | Kumiai Chem Ind Co Ltd | New fungal strain of trichoderma atroviride |
JP2002121103A (en) * | 2000-07-24 | 2002-04-23 | Hokkaido Green Kosan:Kk | Method for providing plant with activity, method for increasing yield and method for using granule |
JP2002138005A (en) * | 2000-10-27 | 2002-05-14 | Idemitsu Kosan Co Ltd | Material for controlling soil pest and method for controlling the same |
JP2008539754A (en) * | 2005-05-11 | 2008-11-20 | エンコート ホールディングス リミテッド | Stabilization of fungal materials |
JP2012095570A (en) * | 2010-10-29 | 2012-05-24 | Institute Of National Colleges Of Technology Japan | Method for producing immobilized trichoderma and method for storing timber |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH06277042A (en) | 1993-03-25 | 1994-10-04 | Mitsui Toatsu Chem Inc | New strain of genus drechslera, and weed-controlling agent and weed-controlling composition containing same |
JPH0764698B2 (en) | 1993-08-04 | 1995-07-12 | 富士技研株式会社 | Soil pest control material |
JP3680263B2 (en) * | 2000-07-13 | 2005-08-10 | カワタ工業株式会社 | Plant disease control agent containing Trichoderma spp. Powder and its production method |
JP4644478B2 (en) | 2004-12-13 | 2011-03-02 | クミアイ化学工業株式会社 | Verticillium lecani YK-920 strain and pest and plant disease control agent using the same |
JP4810151B2 (en) | 2005-07-22 | 2011-11-09 | 出光興産株式会社 | Control agent for diseases occurring during the seedling raising season of rice |
JP5200425B2 (en) | 2006-06-23 | 2013-06-05 | 住友化学株式会社 | Agrochemical active microorganism preparation |
JP5787283B2 (en) | 2008-04-10 | 2015-09-30 | クミアイ化学工業株式会社 | Rice soil disease control agent |
-
2017
- 2017-04-17 WO PCT/JP2017/015504 patent/WO2017188049A1/en active Application Filing
- 2017-04-17 JP JP2018514509A patent/JP6920283B2/en active Active
- 2017-04-17 KR KR1020187033903A patent/KR102455374B1/en active IP Right Grant
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- 2017-04-25 TW TW106113794A patent/TWI731069B/en active
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Patent Citations (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5341424A (en) * | 1976-09-13 | 1978-04-14 | Ricard Jacques | Mold killing agent especially for processing plants |
JPH06192028A (en) * | 1992-12-25 | 1994-07-12 | Hokkaido Green Kosan:Kk | Plant blight controlling microorganism, controlling agent production by using the same, production of controlling agent and using method therefor |
JPH06256125A (en) * | 1993-03-09 | 1994-09-13 | Eezai Seikaken Kk | Controlling agent for white root rot |
JPH0987122A (en) * | 1995-09-21 | 1997-03-31 | Hokkaido Green Kosan:Kk | Disease control for plant |
JPH11225745A (en) * | 1997-11-13 | 1999-08-24 | Kumiai Chem Ind Co Ltd | New fungal strain of trichoderma atroviride |
JP2002121103A (en) * | 2000-07-24 | 2002-04-23 | Hokkaido Green Kosan:Kk | Method for providing plant with activity, method for increasing yield and method for using granule |
JP2002138005A (en) * | 2000-10-27 | 2002-05-14 | Idemitsu Kosan Co Ltd | Material for controlling soil pest and method for controlling the same |
JP2008539754A (en) * | 2005-05-11 | 2008-11-20 | エンコート ホールディングス リミテッド | Stabilization of fungal materials |
JP2012095570A (en) * | 2010-10-29 | 2012-05-24 | Institute Of National Colleges Of Technology Japan | Method for producing immobilized trichoderma and method for storing timber |
Non-Patent Citations (2)
Title |
---|
DAISUKE MAEKAWA ET AL.: "Trichoderma asperelloides SKT-1 Kabu to Sakusan no Kon'yo ni yoru Dojo Byogai Bojo Koka", PHYTOPATHOLOGICAL SOCIETY OF JAPAN SORITSU 100 SHUNEN KINEN TAIKAI PROGRAM-KOEN YOSHI YOKOSHU, vol. 330, 10 March 2015 (2015-03-10), pages 112 * |
NOBUTOSHI MYOJO ET AL.: "Trichoderma asperelloides STK-1 Kabu to Sakusan no Kon'yo Taikei Shori ni yoru Ine Shushi Densensei Byogai ni Taisuru Bojo Koka", PESTICIDE SCIENCE SOCIETY OF JAPAN DAI 40 KAI KINEN TAIKAI PROGRAM KOEN YOSHISHU, 18 March 2015 (2015-03-18), pages 83 * |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111631136A (en) * | 2020-07-14 | 2020-09-08 | 安顺职业技术学院 | Tissue culture and rapid propagation method of dormant buds of eyedrops |
CN112646734A (en) * | 2020-12-31 | 2021-04-13 | 广西壮族自治区林业科学研究院 | Orchid mycorrhizal fungus PF06 and application thereof |
CN112646734B (en) * | 2020-12-31 | 2022-11-29 | 广西壮族自治区林业科学研究院 | Orchid mycorrhizal fungus PF06 and application thereof |
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