Diplodia cupressi A.J.L. Phillips & A. Alves, Fungal Divers. 23: 9. 2006. MycoBank MB510136. Fig. 21.
Ascomata not reported. Conidiomata up to 300 [micro]m diam, solitary, separate, uniloculate, dark brown to black, globose, ostiolate, wall composed of thick-walled textura angularis, becoming thin-walled and hyaline toward the inner region. Conidiophores reduced to conidiogenous cells. Conidiogenous cells hyaline, smooth, holoblastic forming conidia at their tips, proliferating internally giving rise to periclinal thickenings or proliferating percurrently with 1-4 close or widely spaced annellations, formed from the inner wall of the pycnidium, 12.5-20 x 4-4.5 [micro]m. Conidia thick-walled, wall up to 2 [micro]m wide, ovoid with both ends rounded, aseptate, hyaline and remaining so for a long time, becoming brown and 1-septate after discharge from the pycnidia, (21.5-)23.5-28.5(-30.5) x (12-) 13.5-15(-16) [micro]m, 95 % confidence limits = 24.4-25.4 x 13.9-14.5 [micro]m, (av. [+ or -] S.D. of 50 conidia = 24.9 [+ or -] 1.9 x 14.2 [+ or -] 0.9 pm), L/W = 1.76. Spermatophores hyaline, smooth, cylindrical, up to 10 [micro]m long, 2.5-3 [micro]m wide. Spermatogenous cells discrete or integrated, hyaline, smooth, cylindrical, holoblastic or proliferating via determinate phialides with periclinal thickening, 10-14 x 2-2.5 [micro]m. Spermatia hyaline, smooth, aseptate, rod-shaped with rounded ends, 4-5 x 1.5 [micro]m.
Type: Israel, Bet Dagan, dried culture from cankered stems of Cupressus sempervirens, 1986, Z. Solel, holotype IMI 303475.
Culture: CBS 168.87 (ex-type).
Hosts: Cupressus and Juniperus spp. (Alves et al. 2006, Solel et al. 1987).
Known distribution: Cyprus, Greece, Israel, Italy, Morocco, South Africa, Tunisia, USA (De Wet et al. 2009, Alves et al. 2006, Solel et al. 1987).
Notes: Solel et al. (1987) considered this fungus to be a sub-population of Diplodia pinea and named it Diplodia pinea f. sp. cupressi. Swart et al. (1993) challenged this assumption and showed that D. pinea f. sp. cupressi differed morphologically from D. pinea in terms of conidial dimensions, shape, colouration and this was supported by isozyme profiles. The observations of Swart et al. (1993) were supported by ITS sequence data by Zhou & Stanosz (2001). Finally, Alves et al. (2006) introduced the name D. cupressi for the Cypress pathogen. This species is morphologically similar to D. mutila but the conidia of D. cupressi are wider than are typical for D. mutila (Alves et al. 2004).
Diplodia intermedia A.J.L. Phillips, J. Lopes & A. Alves, Persoonia 29: 33. 2012. MycoBank MB19633. Fig. 22.
Ascomata unilocular, solitary or clustered, immersed, partially erumpent when mature, globose, up to 400 [micro]m diam, dark brown to black, thick-walled, wall composed of outer layers of thick-walled, dark brown textura angularis, inner layers of thin-walled, hyaline textura angularis. Ostiole central, circular, nonpapillate, periphysate. Pseudoparaphyses hyaline, branched, septate, constricted at the septum, 2-3 [micro]m wide. Asci clavate, stipitate, bitunicate, containing eight ascospores biseriate in the ascus, 85-160 x 22-28 [micro]m. Ascospores fusiform, widest in the upper third, hyaline, thinwalled, smooth, aseptate, 32-37(-40) x 6-8 [micro]m. Conidiomata pycnidial, stromatic, solitary or clustered, immersed in the host, partially erumpent at maturity, dark brown to black,...