Pestalotiopsis australis Maharachch., K.D. Hyde & Crous, sp. nov. MycoBank MB809731. Fig. 22.
Etymology: Named after the country where it was collected, Australia.
Conidiomata pycnidial in culture on PDA, globose or clavate, aggregated or scattered, semi-immersed or partly erumpent, dark brown to black, up to 400 [micro]m diam; exuding globose, dark brown to black conidial masses. Conidiophores 1-3-septate, sparsely branched at the base, subcylindrical, hyaline, verruculose, up to 25 [micro]m long. Conidiogenous cells discrete or integrated, ampulliform or cylindrical, hyaline, smooth, proliferating 2-4 times percurrently, 20-60 x 2-6 [micro]m, collarette present and slightly flared. Conidia fusoid, ellipsoid, straight to slightly curved, 4-septate, (26-) 27-34(-36) x 7-8.5 [micro]m, [bar.x] [+ or -] SD = 30.8 [+ or -] 2.1 x 7.7 [+ or -] 0.3 [micro]m; basal cell conic to obconic with a truncate base, hyaline, minutely verruculose and thin-walled, 6-10 x [micro]m long; three median cells doliiform, (16-)17-21(-21.5) [micro]m long, [bar.x] [+ or -] SD = 19.1 [+ or -] 1.2 [micro]m, wall minutely verruculose, concolourous, brown, septa darker than the rest of the cell (second cell from the base 5.5-7.5 [micro]m long; third cell 5.5-7.5 [micro]m long; fourth cell 6-8 [micro]m long); apical cell 4-6.5 x [micro]m long, hyaline, cylindrical to subcylindrical, thin--and smooth walled; with 2-3 tubular apical appendages (mostly 3), arising from the apical crest, unbranched, Aliform, (11-) 12-20(-22) [micro]m long, [bar.x] [+ or -] SD = 15.5 [+ or -] 2.7 [micro]m; basal appendage single, tubular, unbranched, centric, 3-7 [micro]m long.
Culture characteristics: Colonies on PDA attaining 35-45 mm diam after 7 d at 25 [degrees]C, with smooth edge, whitish, with sparse aerial mycelium on the surface with black, gregarious conidiomata; reverse similar in colour.
Habitat: On Brabejum stellatifolium, Grevillea sp. and Protea neriifolia x susannae.
Known distribution: Australia and South Africa.
Materials examined: Australia, New South Wales, from Grevillea sp. 12 Oct. 1999, PW. Crous (CBS H-21766, holotype, ex-type culture CBS 114193 = STEU 3011). South Africa, KwaZulu-Natal, from Protea neriifolia x susannae cv. 'Pink Ice', 15 May 1998, L. Swart, culture CBS 114474 = STE-U 1769; ibid., 15 May 1998, L. Swart, culture CBS 111503 = STE-U 1770; on dead leaves of Brabejum stellatifolium, 3 Nov. 2000, S. Lee, PREM 59519, culture CBS 119350 = CMW 20013.
Notes: Pestalotiopsis australis (clade 26; Fig. 5) is a distinct species, which can be isolated from members of Proteaceae. Pestalotiopsis australis is closely related to P scoparia (clade 25; Fig. 5), and is distinguished morphologically from related species by its large conidia.
Pestalotiopsis biciliata Maharachch., K.D. Hyde & Crous, sp. nov. MycoBank MB809732. Fig. 23.
Etymology: Name refers to its two basal appendages.
Conidiomata pycnidial in culture on PDA, globose to clavate, aggregated or scattered, semi-immersed, dark brown to black, up to 300 [micro]m diam; exuding globose, slimy, dark brown conidial droplets. Conidiophores sparsely septate and unbranched or irregularly branched at the base, up to 40 [micro]m long, or reduced to conidiogenous cells. Conidiogenous cells discrete, cylindrical to subcylindrical, hyaline, smooth, tapering to a long, thin neck,...