More than 100 fungal endophyte strains belonging to the family Phacidiaceae were isolated from surface-sterilized Picea rubens Sarg. needles collected from the Acadian Forest Region in New Brunswick, Canada. The strains were characterized morphologically by their asexual states, and phylogenetic analyses were conducted using the nuclear internal transcribed spacer rDNA (ITS) marker and the second largest subunit of ribosomal polymerase II (RPB2). Morphological and phylogenetic data revealed seven species: Darkera cf. parca; Strasseria geniculata; two novel Phacidium species: Phacidium dicosmoanum and Phacidium faciforme; and three novel mo notypic genera described to accommodate distinct species: Calvophomopsis rubenticola, Cornibusella ungulata, and Gloeopycnis protuberans. Further analyses of Darkera spp. were performed with ITS and partial translation elongation factor 1-[alpha] (TEFla), and the results suggest that D. parca is a species complex. Phacidiaceae includes hundreds of known species that are unrepresented by sequence data; therefore, ITS sequences were generated from herbarium material including type specimens of Darkera parca, Phacidium lunatum, and specimens of Mlantophomopsiella, Allantophomopsis, Bulgaria, Phacidium, and Pseudophacidium species. The description of novel species combined with morphological observations and reference sequences will facilitate the identification of conifer endophytes, both from specimens or cultures and in environmental sequence data, and improve our understanding of this large and mostly neglected family. Keywords: coelomycetes, Phacidiopycnis, Phacidium, molecular taxonomy, Phacidiales. Plus de 100 souches d'endophytes fongiques appartenant a la famille des Phacidiaceae ont ete isolees de la surface sterilisee d'aiguilles de Picea rubens Sarg. recoltees dans la region de la foret acadienne au Nouveau-Brunswick, Canada. Les souches ont ete caracterisees d'un point de vue morphologique par leur etat asexue, et des analyses phylogenetiques ont ete realisees avec l'ADNr de l'espaceur interne transcrit (ITS) nucleaire et la deuxieme plus grande sous-unite de l'ARN polymerase II (RBP2). Les donnees morphologiques et phylogenetiques ont mis en evidence sept especes : Darkera cf. parca; Strasseria geniculata; deux nouvelles especes de Phacidium : Phacidium dicosmoanum et Phacidium faciforme, et trois nouveaux genres monotypiques decrits pour accommoder des especes distinctes : Calvophomopsis rubenticola, Cornibusella ungulata et Gloeopycnis protuberans. Des analyses supplementaires de Darkera spp. ont ete realisees avec l'ITS et le facteur d'elongation de la transcription partiel TEF1[alpha], suggerant que D. parca est un complexe d'especes. Les Phacidiaceae comprennent des centaines d'especes connues qui sont sous-representees dans les donnees de sequences; ainsi, les marqueurs ITS ont ete generes a partir du materiel d'herbier, dont des specimens types Darkeraparca, Phacidium lunatum et des specimens de Allantophomopsiella, Allantophomopsis, Bulgaria, Phacidium, et Pseudophacidium. La description d'especes nouvelles combinee aux observations morphologiques et aux sequences de reference facilitera l'identification d'endophytes des coniferes, tant de specimens que de cultures et dans des donnees de sequences environnementales, et ameliorera notre comprehension de cette grande famille essentiellement negligee. [Traduit par la Redaction] Mots-cles : coelomycetes, Phacidiopycnis, Phacidium, taxonomie moleculaire, Phacidiales.