Independent data validation for selected genes by in situ hybridization on B. gunnisoniana ovules.
Data validation for selected genes found expressed in the Boechera AIC but not the Arabidopsis MMC. Scale bars are 20 µm, arrows point to the AICs. In situ hybridizations on B. gunnisoniana ovule sections were performed with antisense probes (A–C, E, F, H, I, K, M, N) or sense probes as controls (D, G, J, L, O) for the transcription factors AT1G06170, a basic helix-loop-helix (bHLH) DNA-binding superfamily protein (A–D), AT1G28050, a B-BOX DOMAIN PROTEIN 13 (E–G), and AT1G76580 a Squamosa promoter-binding protein-like (SBP domain) transcription factor family protein (H–J), an oligopeptide transporter, AT1G59740 (K,L), and AT1G14900, encoding the HIGH MOBILITY GROUP A protein.
More »