Fig 1.
Microphotograph showing the grossing appearance of the lesion that showed multiple black grains.
Fig 2.
Microphotograph showing multiple black grains surrounded by granulation tissue with marked histiocytic and mixed inflammatory cellular infiltrates (HE 10Ă—).
(A) Grains of Chaetomium spp. showed abundant extracellular matrix, which was yellow to brown in colour, and the fungus hyphae are located at the periphery of the matrix with short filamentous structure. (B) The filamentous pattern of M. mycetomatis grains consists of brown septate and branched hyphae at the centre and periphery with long filament. (C, D) Microphotograph of LPCB mount showing ascoma and ascospore cells resembling the typical Chaetomium spp. cells (C) and conidia of M. mycetomatis (D). HE, haematoxylin–eosin; LPCB, lactophenol cotton blue.
Table 1.
The different characteristic features of C. atrobrunneum and M. mycetomatis.
Table 2.
MIC of antifungal agents against C. atrobrunneum MRC9 isolate by Sensititre YeastOne test.
Fig 3.
Neighbor-joining phylogenetic tree of ITS sequences of C. atrobrunneum BG9 strain (in red) and Chaetomium and Madurella strains of both clinical and environmental origin, downloaded from NCBI Database.
A Papulaspora equi strain (CBS 128687) was used to root the tree. The black arrow indicates the Chaetomium clade. ITS, internal transcribed spacer; NCBI, National Center for Biotechnology Information.
Table 3.
Eumycetoma-causing agents.