Table 1.
Molecular markers, primers and PCR conditions used in this study.
Figure 1.
General morphological characteristics of Escovopsis kreiselii CBS 139320 (= LESF53).
A-D: cultures grown in PDA, MA2%, CMD and SNA after 14 days at 25°C, respectively; E: Conidiophores pattern formation on the aerial mycelia; F-H: Conidiophore branching pattern; I-L: Percurrent conidiogenous cells; M: Chlamydospores developed in chains and N: Globose to subglobose conidia.
Figure 2.
SEM of Escovopsis kreiselii CBS 139320 (= LESF53) showing morphological aspects.
Slide cultures grown in PDA and SNA for five days at 25°C. A-B: Conidiophore growing patterns on the aerial mycelia; C: Conidiophore branching pattern; D: Chlamydospores; E-I: Percurrent conidiogenous cells showing conidia attached to the cells by denticles; J-M: Conidia. J: Conidia with attached denticle; K-L: Conidia without denticles and M: smooth walled conidia.
Figure 3.
Phylogenetic position of Escovopsis kreiselii within the Escovopsis clade based on tef1 sequences reconstructed using Bayesian Inference.
All Escovopsis species described so far are denoted in bold. In addition to the newly described species, forty-three tef1 sequences representing all Escovopsis morphotypes used in previous studies were retrieved from GenBank. Sequences of tef1 from other Hypocreaceae were used as outgroup. The voucher accession numbers in culture collections follow the taxon names. GenBank accessions and the ant species from which the fungi were isolated are given in parentheses. Different colors indicate the fungal morphotypes found in the Escovopsis clade. Bootstrap values from ML analyses are also indicated from a similar topology. Only PP and bootstrap values ≥ 0.5 or 50 are shown. Phylogeny based on Gerardo et al. [9]. T: ex-type strains. Bar: 0.05 substitutions per nucleotide position.
Figure 4.
Phylogeny of described species within the Escovopsis clade.
This tree was generated from Bayesian analyses of two different markers: ITS and tef1. Sequences of other Hypocreaceae species were used as outgroup. The voucher accession numbers in culture collections follow the taxon names, and GenBank accessions and the ant species from which the fungi were isolated are given in parentheses. The new species, Escovopsis kreiselii is denoted in bold. ML analyses resulted in an identical topology and ML bootstraps values are shown above nodes. T: ex-type strains. Bar: 0.05 substitutions per nucleotide position.