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Fig 1.

Actinobacteria genera isolated from brackish water sponges.

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Fig 2.

Actinobacteria genera isolated from clear tropical waters.

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Fig 3.

Phylogenetic tree of Streptomyces spp.

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Fig 4.

PCA of streptomyces spp.

The top figure highlights strains in biological duplicate as denoted by the shapes and colors. The bottom figure distinguishes between brackish/clear isolates.

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Fig 5.

PCA of all bacterial strains.

95% confidence intervals are indicated by shaded area. (A) Samples are indicated based on clear [C] or brackish [B] water collection sites. (B) Samples are indicated based on bacterial genus: Solwaraspora [Sol], Micromonospora [Mic], Streptomyces [Str], or Verrucosispora [Ver]. Note: Streptomyces spp. were processed differently than the other three genera.

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Fig 6.

PLS-DA analysis of strains based on water.

Samples are indicated based on clear [C] or brackish [B] water collection sites. 95% confidence intervals are indicated by shaded area. Note: Streptomyces spp. were processed differently than the other three genera.

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Fig 6 Expand

Fig 7.

PLS-DA where groups were based on the bacterial genus.

Samples are indicated based on bacterial genus: Solwaraspora [Sol], Micromonospora [Mic], Streptomyces [Str], or Verrucosispora [Ver]. 95% confidence intervals are indicated by shaded area. Note: Streptomyces spp. were processed differently than the other three genera.

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Fig 7 Expand

Fig 8.

PCA scores plot for Micromonospora spp.

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Fig 9.

Representative sample of the hierarchal cluster of Micromonospora spp.

Isolates collected from sponges of the same genera share similar colors, and clear tropical samples are represented in bold and italic formatting. Full tree is available in S2 Fig.

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Fig 10.

PCA scores plot for Verrucosispora spp.

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Fig 10 Expand

Fig 11.

Representative sample of the hierarchal cluster of Verrucosispora spp.

Isolates collected from sponges of the same genera share similar colors, and clear tropical samples are represented in bold and italic formatting. Full tree is available in S3 Fig.

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Table 1.

Biological activity data.

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Table 1 Expand