CN101658186B - Verticillium lecanii quick-release dispersible tablet and preparation method thereof - Google Patents
Verticillium lecanii quick-release dispersible tablet and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a verticillium lecanii quick-release dispersible tablet and a preparation method thereof. Verticillium lecanii concentrated fermentation liquor or dry spore powder thereof is used as a main effective component. Synergist, surface active agent, protective agent, disintegrating agent, wetting agent, filler and lubricant are added as well. The preparation method is implemented by the following steps: 2/3 disintegrating agent and other auxiliary agents, together with verticillium lecanii, are added into a mixer for uniform mixing according to a certain proportion; then the wetting agent with an appropriate amount is added for mixing so that the mixture obtained from the previous step is changed into a wet material; the wet material then undergoes 20-mesh screening so as to be prepared into wet grains; after 24-48-hour drying at the normal temperature in a relatively germfree condition, the rest 1/3 disintegrating agent is added into the dry grains, and then the grains are weighed; the lubricant with a weight accounting for 0.5-0.6% of the grain weight is added for full and uniform mixing, and then the grains are sent to a tablet machine to be pressed into large tablets so that the finished product can be obtained. The verticillium lecanii quick-release dispersible tablet has simple production process and wide material source, is easy for industrial production, can be quickly disintegrated in water to release effective components, takes effect quickly, does not cause dust pollution, has a wide range of prevention and cure and can kill insect and bacteriostasis simultaneously.
Description
Technical field
The invention belongs to a kind of fungi microbe pesticide producing process technology in biological control field, particularly relate to concrete Verticillium lecanii pesticide-germicide microbial pesticide.
Background technology
Verticillium lecanii found since 1861 until the fifties in last century of just further investigation abroad, and applied, and existing so far tens kinds of commodity have been realized suitability for industrialized production, listing, and the Trialeurodes vaporariorum Westwood effect that is used to prevent and treat cucumber and tomato is remarkable.And China just began one's study from the seventies in last century, the small area applications that test products is arranged, but do not see that relevant large tracts of land field applies report, so far still there is not the goods registration registration, main cause is that the technology and the formulation development that are fit to suitability for industrialized production relatively lag behind, thereby has influenced its further commercialization.
Verticillium lecanii is all insect pathogenic fungus very widely of a kind of host range and geographical distribution, be mainly used in control homoptera pest such as aphid, aleyrodid etc., also insects such as orthoptera, Semiptera, Lepidoptera, coleoptera be can prevent and treat, mite class and some phytopathogens also can be used for preventing and treating.Its great advantage is: free from environmental pollution, to people and animals, environment and natural enemies security, genetic stability is strong, easily produce, prevention and treatment range is wide, and desinsection is antibacterial again, especially the most obvious to the helpless sucking pest control efficiency of other biological insecticides simultaneously, and can in controlling object, produce the cause of disease epidemic disease and reach the effect of long-term control, have higher development and be worth and the wide development prospect.But its pest control is bred and desinsection in pest body with conidium or mycelium, it is higher when so it is used to environmental factor especially temperature, humidity requirement, control efficiency also is subject to the ultraviolet ray influence, and its general preparation storage life is too short, in order to overcome the above-mentioned shortcoming of Verticillium lecanii, giving full play to its advantage, is comparatively simple measure by formulation processing and interpolation auxiliary agent.But Verticillium lecanii is the organism that lives, environment (temperature, humidity, illumination etc.) is comparatively responsive to external world for it, its formulation processing is more more complicated than chemical insecticide, therefore, it is even more important that the developmental research of novel environment friendly formulation just seems, it also is that necessary process is produced in the further commercialization of Verticillium lecanii.
At present, it is domestic that research still is in the exploratory stage about its formulation and auxiliary agent thereof, correlative study mainly is to make wetting powder, but it causes dust pollution easily, dose controlled is poor, thereby cause waste and the producer and user's safety coefficient is reduced, and the main active ingredient of wetting powder that prior art is produced is the mixture of its spore, mycelium and medium, active constituent content is too low in the unit volume, these have all improved agricultural cost indirectly, have limited its large-scale promotion application.In addition, under existing technology condition, the drying process of principal component is a production link that cost is very high in its wetting powder production, has limited its further large-scale industrial production.
Summary of the invention
The object of the invention is to provide a kind of processing that is easy to, and cost is lower, and it is convenient to use, store and transport, long shelf-life, and free from environmental pollution, virulence is strong, the verticillium lecanii quick-release dispersible tablet that desinsection is fungi-proofing again.
Another object of the present invention is to provide the preparation method of verticillium lecanii quick-release dispersible tablet.
Verticillium lecanii quick-release dispersible tablet of the present invention is that the main ingredient of 60%-85% and the auxiliary agent of 15%-40% are formed by weight percentage; main ingredient is Verticillium lecanii xerospore powder or Verticillium lecanii concentrated broth, and auxiliary agent comprises surfactant, spore protectant, uv-protector, synergist, disintegrant, filler, wetting agent and lubricant.
Verticillium lecanii quick-release dispersible tablet preparation method of the present invention is: all solids auxiliary agent is all crossed 100 mesh sieves in advance, the liquid adjuvants filter and remove residue, take by weighing the main ingredient that weight percentage is 60%-85%, successively adding weight percentage in mixer is the 1-5% surfactant, weight percentage is a 0.1-0.2% spore protectant, weight percentage is the 0.01-0.02% uv-protector, weight percentage is the 0.2-1% synergist, weight percentage is that 3.33-10% disintegrant and weight percentage are the 7.79-17.58% filler, fully stirring and evenly mixing is 60 minutes, adding weight percentage again is the 0.4-0.6% wetting agent, continued stirring and evenly mixing 30 minutes, (that pinches is agglomerating to make uniform moist wood, promptly loosing of pressure is degree), cross 20 mesh sieves and make wet granular, under the 20-22 ℃ of aseptic relatively condition dry 24-48 hour, extremely dried particle water content is weight percentage 5-6%, again weight percentage is added in the dried particle for the 1.67-5% disintegrant, after claiming the particle gross weight, add weight percentage and be the abundant mixing of lubricant 30 minutes of particle gross weight 0.5-0.6%, enter tablet press machine and be pressed into the anticipation shaped tablets, get product with the pressure of 2-3 ton;
Surfactant is selected from a kind of in lauryl sodium sulfate, Tween-80, Arlacel-80, Arlacel-60, monoglyceride, lauric acid one ester or the sodium hexadecyl sulfate,
The spore protectant is selected from a kind of in L-serine, sodium alginate, skim milk, sodium hydrogen phosphate powder, ascorbic acid or the molasses,
Uv-protector be selected from fluorescein sodium, methyl green, rhodamine B, aesculin, folic acid or Congo red in a kind of,
Synergist is selected from a kind of in chitinase, chlorogenic acid, polyphenol oxidase, mercaptoethanol, urea, protein inhibitor or the organic acid, and protein inhibitor selects rotenone, and organic acid selects p-aminobenzoic acid);
Disintegrant is selected from a kind of in sodium carboxymethylcellulose, Ac-Di-Sol, polyvinylpyrrolidone, crospolyvinylpyrrolidone, low-substituted hydroxypropyl cellulose sodium or the dried starch, perhaps be selected from the mixture of Ac-Di-Sol and crospolyvinylpyrrolidone, the ratio of Ac-Di-Sol and crospolyvinylpyrrolidone is 1: 2;
Filler is a kind of in microcrystalline cellulose, pregelatinized starch, xylitol, sodium alginate or the calcium sulphate, perhaps be selected from the mixture of clay and sucrose, perhaps be selected from maltose and dextrin mixture, the ratio of clay and sucrose is 10: 1, and the ratio of maltose and dextrin is 1: 8;
Lubricant is selected from a kind of in dolomol, talcum powder or the superfine silica gel powder;
Pregelatinized starch is purchased in Qufu City sky profit pharmaceutic adjuvant Co., Ltd.
Main ingredient is selected Verticillium lecanii xerospore powder, and its preparation process is the Verticillium lecanii spore that will cover with in the potato medium with aseptic kaolin wash-out, and strips down together with lawn, and air drying is 7 days under aseptic condition, pulverizes, and mistake 100 mesh sieves promptly; Wetting agent be selected from and tell-80 or Tween-60 in a kind of.
Main ingredient is selected the Verticillium lecanii concentrated broth, its preparation process is that inclining supernatant with Verticillium lecanii liquid fermentate with 6000 rev/mins speed centrifugal 20 minutes, and sediment divides with the supernatant of its 1.5 times of volumes and washes out for three times, merge this eluate of three times, promptly.
When main ingredient is selected the Verticillium lecanii concentrated broth, the adding mode of itself and wetting agent is that the spray bottle with aperture 0.1cm is spilled into Verticillium lecanii concentrated broth and wetting agent in the mixer with Sprayable, make uniform moist wood, wetting agent is the centrifuged supernatant of Verticillium lecanii concentrated broth.
Bacterial strain: the used bacterial classification of the present invention is that Chinese microorganism strain preservation center provides, bacterium number is CGMCG3.4431, CGMCG3.4505, the rejuvenation by Trialeurodes vaporariorum Westwood and aphid if instar larvae is transferred, if being about to former bacterial classification transfers in the Trialeurodes vaporariorum Westwood of artificial feeding on the instar larvae, examine, with the polypide of death be connected on the PDA medium 25 ℃ constant temperature culture 7-10 days, dilute with sterile water after selecting the polypide fragmentation of covering with white hair, carry out separation and purification, the all higher bacterial strain of host's property and virulence is therefrom obtained in the virulence screening.Rejuvenation of the present invention obtains 3 strain Verticillium lecaniis, and their host's obligate, insecticidal toxicity and genetic stability is all very strong, and 26 ℃ of constant temperature culture are 17 days on the PDA medium, and average sporulation quantity can reach 1.3 * 10
11Individual spore/g, 26 ℃ of constant temperature culture of liquid shaking bottle 17 days, average sporulation quantity can reach 3.6 * 10
10Individual spore/ml.
The present invention selects to make the quick disintegration of tablet can guarantee the suitable auxiliary agent of Verticillium lecanii spore activity again, and the granule size of control main ingredient and auxiliary agent, is the key that guarantees this quick-release dispersible tablet quality.
The finished product that the present invention obtained is a verticillium lecanii quick-release dispersible tablet, and its production technology is simple, and main ingredient weighs 1.3g/ sheet, main ingredient average content 1.64 * 10 with Verticillium lecanii xerospore powder or its concentrated broth, the average sheet of product
10Individual spore/sheet.Compare with conventional tablet, its disintegration rate is fast, and disintegration rapidly in water discharges effective ingredient, it is dispersed in forms suspension in the water, and is both sprayable in various crop surface, also can directly quick-release dispersible tablet be disseminated in the paddy field, extremely easy to use.The virulence of this product is strong, lasting period is long, prevention and treatment range is wide, and desinsection is fungi-proofing again simultaneously, especially Trialeurodes vaporariorum Westwood and botrytis cinerea is had special efficacy, strong to people and animals, bad border and natural enemies security, dose controlled, no dust pollution, storing are convenient, and the shelf-life can reach more than 2 years and (prolong many compared with similar products), and the normal temperature sealing keeps away cloudy the preservation and gets final product, if 4 ℃ of preservations, the shelf-life is longer.Control efficiency to Trialeurodes vaporariorum Westwood reaches more than 98%, also can reach more than 85% the preventive effect of botrytis cinerea, and the lasting period extended to more than 15 days.
Embodiment
The following examples can make those skilled in the art more fully understand the present invention, but do not limit the present invention in any way.
Embodiment 1-5 main ingredient is a Verticillium lecanii xerospore powder
Main ingredient is selected Verticillium lecanii xerospore powder, and its preparation process is the Verticillium lecanii spore that will cover with in the potato medium with aseptic kaolin wash-out, and strips down together with lawn, and air drying is 7 days under aseptic condition, pulverizes, and mistake 100 mesh sieves promptly.
The product population that to prepare of component design and determine at first, get the raw materials ready by the listed formula ratio of table one, the all solids auxiliary agent is all crossed 100 mesh sieves in advance, take by weighing main ingredient, in mixer, successively add surfactant, the spore protectant, uv-protector, synergist, 2/3 disintegrant and filler, fully stirring and evenly mixing is 60 minutes, add wetting agent again, continue stirring and evenly mixing 30 minutes, and made uniform moist wood (that pinches is agglomerating, and promptly loosing of pressure is degree), cross 20 mesh sieves and make wet granular, under the 20-22 ℃ of aseptic relatively condition dry 24-48 hour, to dried particle water content be weight percentage 5-6%, will remain 1/3 disintegrant again and add in the dried particle, after claiming the particle gross weight, added the abundant mixing of lubricant 30 minutes, and entered tablet press machine and be pressed into the anticipation shaped tablets, get product with the pressure of 2-3 ton;
Table one, main ingredient are the quick-release dispersible tablet formula ratio of Verticillium lecanii xerospore powder
Note: the equal pre-preparation of each dispersing tablet is 80 kilograms in the table, PPVP---crospolyvinylpyrrolidone, CCMC-Na---Ac-Di-Sol, CMC-Na---sodium carboxymethylcellulose, PVP---polyvinylpyrrolidone.
Embodiment 6-10: the preparation main ingredient is the Verticillium lecanii concentrated broth.
Main ingredient is selected the Verticillium lecanii concentrated broth, its preparation process is that inclining supernatant with Verticillium lecanii liquid fermentate with 6000 rev/mins speed centrifugal 20 minutes, and sediment divides with the supernatant of its 1.5 times of volumes and washes out for three times, merge this eluate of three times, promptly.
At first component design and definite product population that will prepare are got the raw materials ready by the listed formula ratio of table two, and all solids auxiliary agent is all crossed 100 mesh sieves, liquid adjuvants filter and remove residue in advance; Successively add surfactant, spore protectant, uv-protector, synergist, 2/3 disintegrant and filler in mixer, fully stirring and evenly mixing is 60 minutes; Spray bottle with aperture 0.1cm is spilled into Verticillium lecanii concentrated broth and wetting agent in the mixer with Sprayable, makes uniform moist wood, and wetting agent is the centrifuged supernatant of Verticillium lecanii concentrated broth.Continued stirring and evenly mixing 30 minutes, (that pinches is agglomerating to make uniform moist wood, promptly loosing of pressure is degree), cross 20 mesh sieves and make wet granular, under the 20-22 ℃ of aseptic relatively condition dry 24-48 hour, extremely dried particle water content is weight percentage 5-6%, to remain 1/3 disintegrant again and add in the dried particle, claim the particle gross weight after, added the abundant mixing of lubricant 30 minutes, enter tablet press machine and be pressed into the anticipation shaped tablets, get product with the pressure of 2-3 ton;
Table two, main ingredient are the quick-release dispersible tablet formula ratio of Verticillium lecanii concentrated broth
Note: the equal pre-preparation of each dispersing tablet is 80 kilograms in the table, PPVP---crospolyvinylpyrrolidone, CCMC-Na---Ac-Di-Sol, CMC-Na---sodium carboxymethylcellulose, PVP---polyvinylpyrrolidone.Among the above embodiment, it is short disintegration time limited that embodiment 5 and 9 makes product, long shelf-life, and preventive effect is good, compares better with other embodiment product, so adopted by the following examples.
Embodiment 11: filling a prescription is: Verticillium lecanii concentrated broth (main ingredient) 68kg, wetting agent are that the centrifuged supernatant weight percentage of main ingredient is 1%, filler is for the starch weight percentage is 13.5%, lubricant is that the dolomol weight percentage is 0.5%, the preparation method is with embodiment 1, make the heavy 1.38g/ sheet of the average sheet of product, main ingredient average content 0.33 * 10
10Individual spore/sheet.
Characteristic relatively
Survey its disintegration time limited with changeing the basket method, temperature is controlled at 24-25 ℃, and dilution-plate method is surveyed the viable count of its normal temperature preservation after one, two year.
Press embodiment 9 preparation verticillium lecanii quick-release dispersible tablets, the heavy 1.3g/ sheet of average sheet, main ingredient average content 1.64 * 10
10Individual spore/sheet.
The key property of table three, verticillium lecanii quick-release dispersible tablet and conventional tablet relatively
The result shows: the embodiment of the invention 9 effects are better than embodiment 11.
Verticillium lecanii quick-release dispersible tablet is to the field control effect of Trialeurodes vaporariorum Westwood and gray mold and the test of the control efficiency in the shelf-life.
In the greenhouse of cultivation poinsettia; choose serious biennial and poinsettia plant 360 strains of having bloomed of Trialeurodes vaporariorum Westwood and gray mold harm; be divided into 12 rectangle sub-districts; six is test drug (LAJ dispersing tablet 1 and LAJ dispersing tablet 2) sub-district; three is damage by disease and insect positive control sub-district; three is the blank sub-district; add guard row between four groups of sub-districts; each sub-district 30 strain; every sub-district is got 10 strains at random; whenever select good strains in the field for seed three (from plant; in; following three positions are chosen) borer population is more and have the blade of botrytis cinerea scab to write down blade area; basis lesion area and the basic borer population and the mark of listing; then; spray experimental drug simultaneously; contrast medicine and clear water spray from the blade two sides, with the blade degree of being that drips.A few days ago sheltering from heat or light, preserving moisture after the dispenser, and after dispenser live borer population, lesion area and have or not poisoning of the 3rd, five, seven, 15 day observed and recorded.Test of pesticide effectiveness method in shelf-life is the same.Result of the test sees Table four, table five.
Table four, verticillium lecanii quick-release dispersible tablet are to the field control effect of Trialeurodes vaporariorum Westwood
Annotate: this tests used tablet specification is the heavy 1.3g/ sheet of average sheet, active ingredient average content 1.64 * 10
10Individual spore/sheet; The anti-correcting controling effect explanation of effectiveness dispenser at that time after five days in shelf-life; The verticillium lecanii quick-release dispersible tablet of embodiment 9 preparations is pressed in 1 representative of LAJ dispersing tablet, and the verticillium lecanii quick-release dispersible tablet of embodiment 5 preparations is pressed in 2 representatives of LAJ dispersing tablet.
Table five, verticillium lecanii quick-release dispersible tablet are to the field control effect of gray mold
Annotate: this tests used tablet specification is the heavy 1.3g/ sheet of average sheet, active ingredient average content 1.64 * 10
10Individual spore/sheet; State of an illness investigation was carried out after dispenser on the 9th day; The anti-effectiveness control efficiency explanation behind the dispenser Ninth Heaven at that time in shelf-life; The verticillium lecanii quick-release dispersible tablet of embodiment 9 preparations is pressed in 1 representative of LAJ dispersing tablet, and the verticillium lecanii quick-release dispersible tablet of embodiment 5 preparations is pressed in 2 representatives of LAJ dispersing tablet.
From table four and table five experimental result, verticillium lecanii quick-release dispersible tablet can be prevented and treated Trialeurodes vaporariorum Westwood and the gray mold on the poinsettia simultaneously, can reach more than 98% the control efficiency of Trialeurodes vaporariorum Westwood, and the lasting period is long, and is more effective than its positive control.Though the control efficiency to gray mold does not have corresponding positive control advantage, its relative control efficiency also can reach more than 85%.After preserving 2 years, controlling object is still had preventive effect preferably.
The biological medicine of the present invention is effective, and avirulence can substitute virose chemical medicine Ah. pyridine worm fully and narrow or phonetic mould amine.
The verticillium lecanii quick-release dispersible tablet of the present invention's preparation, being generally 20 dissolves with the 20-30 premium on currency, carry out the crop surface spraying or directly be sprinkled in the paddy field, be used for the control of hundreds of insects and multiple diseases in grain, vegetables, flowers, fruit and the forestry, be particularly useful for the control of sucking pest such as the Trialeurodes vaporariorum Westwood of common refractory again in the industrialized agriculture, various aphids and gray mold, powdery mildew etc.
Claims (4)
1. the preparation method of a verticillium lecanii quick-release dispersible tablet, it is characterized in that it comprises the following steps: by weight percentage is that the main ingredient of 60%-85% and the auxiliary agent of 15%-40% are formed, main ingredient is Verticillium lecanii xerospore powder or Verticillium lecanii concentrated broth, and auxiliary agent comprises surfactant, spore protectant, uv-protector, synergist, disintegrant, filler, wetting agent and lubricant;
The all solids auxiliary agent is all crossed 100 mesh sieves in advance, take by weighing the main ingredient that weight percentage is 60%-85%, successively adding weight percentage in mixer is the 1-5% surfactant, weight percentage is a 0.1-0.2% spore protectant, weight percentage is the 0.01-0.02% uv-protector, weight percentage is the 0.2-1% synergist, weight percentage is that 3.33-10% disintegrant and weight percentage are the 7.79-17.58% filler, fully stirring and evenly mixing is 60 minutes, adding weight percentage again is the 0.4-0.6% wetting agent, continued stirring and evenly mixing 30 minutes, make uniform moist wood, cross 20 mesh sieves and make wet granular, under the 20-22 ℃ of aseptic relatively condition dry 24-48 hour, extremely dried particle water content is weight percentage 5-6%, again weight percentage is added in the dried particle for the 1.67-5% disintegrant, after claiming the particle gross weight, add weight percentage and be the abundant mixing of lubricant 30 minutes of particle gross weight 0.5-0.6%, enter tablet press machine and be pressed into the anticipation shaped tablets, get product with the pressure of 2-3 ton;
Surfactant is selected from a kind of in lauryl sodium sulfate, Tween-80, Arlacel-80, Arlacel-60, monoglyceride or the sodium hexadecyl sulfate,
The spore protectant is selected from a kind of in L-serine, sodium alginate, skim milk, sodium hydrogen phosphate powder, ascorbic acid or the molasses,
Uv-protector be selected from fluorescein sodium, methyl green, rhodamine B, aesculin, folic acid or Congo red in a kind of,
Synergist is selected from a kind of in chitinase, chlorogenic acid, polyphenol oxidase, mercaptoethanol, urea, protein inhibitor or the organic acid, and protein inhibitor selects rotenone, and organic acid selects p-aminobenzoic acid;
Disintegrant is selected from a kind of in sodium carboxymethylcellulose, Ac-Di-Sol, polyvinylpyrrolidone, crospolyvinylpyrrolidone, low-substituted hydroxypropyl cellulose or the dried starch, perhaps is selected from
The mixture of Ac-Di-Sol and crospolyvinylpyrrolidone, the ratio of Ac-Di-Sol and crospolyvinylpyrrolidone are 1: 2;
Filler is a kind of in microcrystalline cellulose, pregelatinized starch, xylitol, sodium alginate or the calcium sulphate, perhaps be selected from the mixture of clay and sucrose, perhaps be selected from maltose and dextrin mixture, the ratio of clay and sucrose is 10: 1, and the ratio of maltose and dextrin is 1: 8;
Lubricant is selected from a kind of in dolomol, talcum powder or the superfine silica gel powder.
2. the preparation method of verticillium lecanii quick-release dispersible tablet according to claim 1, it is characterized in that: main ingredient is a Verticillium lecanii xerospore powder, preparation process is the Verticillium lecanii spore that will cover with in the potato medium with aseptic kaolin wash-out, and strip down together with lawn, air drying is 7 days under aseptic condition, pulverize, cross 100 mesh sieves promptly; Wetting agent is selected from a kind of in Tween-80 or the Tween-60.
3. the preparation method of verticillium lecanii quick-release dispersible tablet according to claim 1, it is characterized in that: main ingredient is the Verticillium lecanii concentrated broth, preparation process is with Verticillium lecanii liquid fermentate with 6000 rev/mins speed centrifugal 20 minutes, incline and supernatant, sediment divides with the supernatant of its 1.5 times of volumes and washes out for three times, merges this eluate of three times; Promptly;
The adding mode of Verticillium lecanii concentrated broth and wetting agent is that the spray bottle with aperture 0.1cm is spilled into Verticillium lecanii concentrated broth and wetting agent in the mixer with Sprayable, make uniform moist wood, wetting agent is the centrifuged supernatant of Verticillium lecanii concentrated broth.
4. the verticillium lecanii quick-release dispersible tablet that makes according to claim 2 or 3 described preparation methods.
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| CN102428965A (en) * | 2011-09-22 | 2012-05-02 | 中国水稻研究所 | Specialized form spore powder of helminthosporium carposaprum echinochloa crusgalli |
| BR112014014702A2 (en) * | 2011-12-19 | 2017-08-22 | Novozymes Bioag As E Novozymes Biologicals Inc | METHOD TO CONTROL A PEST, AND, BIOPESTICIDE |
| CO7290160A1 (en) * | 2014-11-28 | 2015-06-10 | Corporacion Colombiana De Investigacion Agropecuaria Corpoica | Pesticide Compositions |
| CN106912501B (en) * | 2017-05-02 | 2018-02-13 | 中国农业科学院植物保护研究所 | The compounded pesticides of Lecanicillium lecanii and rotenone |
| CO2017012690A1 (en) * | 2017-12-11 | 2019-06-11 | Corporacion Colombiana De Investig Agropecuaria Agrosavia | Solid compositions comprising microorganisms with biocidal activity, fertilizer and / or plant growth promoter |
| CN108513976A (en) * | 2018-03-28 | 2018-09-11 | 方建波 | A kind of preparation method of granular pesticide |
| CN114304188B (en) * | 2021-12-15 | 2023-02-28 | 宁夏大学 | Verticillium lecanii dispersible oil suspending agent and preparation method and application thereof |
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| CN101444226A (en) * | 2008-12-26 | 2009-06-03 | 沈阳农业大学 | Recipe of Verticillium lecanii(Zimmerman)Viegas water dispersible granules used for preventing and controlling aphids and greenhouse whiteflies and preparation method |
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| CN1615703A (en) * | 2003-11-14 | 2005-05-18 | 谢明 | High efficiency compound preparation for preventing and controlling piercing-aucking type pest in greenhouse |
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