CN102086182A - Petasites tatewakianus Kitam. extract as well as preparation method and application thereof - Google Patents
Petasites tatewakianus Kitam. extract as well as preparation method and application thereof Download PDFInfo
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Abstract
The invention relates to the technical field of medicine, in particular to Petasites tatewakianus Kitam. extract and novel sesquiterpene lactone compound bakkenolide VIIa separated from the Petasites tatewakianus Kitam. extract. The chemical structural formula of the bakkenolide VIIa is as shown in the specification. Animal experiments and in vitro experiments prove that the Petasites tatewakianus Kitam. extract or bakkenolide VIIa has the activities of preventing cerebral isochemia, myocardial isochemia, allergic rhinitis, tumor, dysmnesia and the like, thus the Petasites tatewakianus Kitam. extract or the bakkenolide VIIa can be used for preparing medicaments capable of preventing heart cerebrovascular diseases, allergic rhinitis, tumor, senile dementia, tristimania, histamine and/or leukocyte component-related diseases. The preparation method is simple, and the cost is low; and the invention provides a novel medicament source for preventing the heart cerebrovascular diseases, allergic rhinitis, tumor, senile dementia, tristimania, histamine and/or leukocyte component-related diseases.
Description
Technical field
The present invention relates to medical technical field, is that palm leaf Rhizome of Japanese Butterbur extract reaches new sesquiterpene lactones compound Rhizoma Petasitis tricholobi lactone VII a and their application in preparation control cardiovascular and cerebrovascular diseases, allergic rhinitis, tumour, senile dementia, dysthymia disorders, histamine and/or leukotrienes composition relative disease medicine that therefrom separation obtains.
Background technology
Palm leaf Rhizome of Japanese Butterbur Petasites tatewakianus Kitam. is perennial herb, mainly is distributed in the Xiaoxinanlin Mountains, China northeast and Changbaishan area, and Muscovite the Far East Area and Sakhalin also have distribution.
The butterbur plant is as plant amedica, and the extract of its rhizome is used for the treatment of migraine, the urogenital tract spasm, and asthma, allergic rhinitis etc., sesquiterpenoids is considered to its main effective constituent.But do not see that so far relevant palm leaf Rhizome of Japanese Butterbur extract has the report of control cardiovascular and cerebrovascular diseases, allergic rhinitis, tumour, senile dementia, dysthymia disorders, histamine and/or leukotrienes composition relative disease, does not see the report that is separated to Rhizoma Petasitis tricholobi lactone VII a from palm leaf Rhizome of Japanese Butterbur extract yet.
Summary of the invention
The invention provides a kind of leaf Rhizome of Japanese Butterbur extract of slapping and reach the new sesquiterpene lactones compound Rhizoma Petasitis tricholobi lactone VII a that therefrom separation obtains, and they are used to prepare the new purposes of control cardiovascular and cerebrovascular diseases, allergic rhinitis, tumour, senile dementia, dysthymia disorders, histamine and/or leukotrienes composition relative disease medicine, the chemical structural formula of said Rhizoma Petasitis tricholobi lactone VII a is as follows:
Studies show that the activeconstituents that the present invention slaps leaf Rhizome of Japanese Butterbur extract is mainly Rhizoma Petasitis tricholobi lactone VII a, same Rhizoma Petasitis tricholobi lactone, Rhizoma Petasitis tricholobi lactone-I a, Bakkenolide III a, Bakkenolide-, Bakkenolide B, Bakkenolide D and Bakkenolide L.
Palm leaf Rhizome of Japanese Butterbur extract of the present invention and Rhizoma Petasitis tricholobi lactone VII a preparation method are as follows:
1. leaf Rhizome of Japanese Butterbur extract is slapped in preparation
After will slapping the dry rhizome pulverizing of sawfly bucket dried vegetable, water or aqueous ethanolic solution diacolation routinely extract or soak and extract, and the extracting solution concentrating under reduced pressure becomes medicinal extract, uses petroleum ether extraction behind the water suspendible then, with the extraction liquid concentrating under reduced pressure, must slap leaf Rhizome of Japanese Butterbur extract.
2. prepare Rhizoma Petasitis tricholobi lactone VII a
With above-mentioned palm leaf Rhizome of Japanese Butterbur extract silica gel H (60 types, Qingdao Haiyang Chemical Industry Group Corp.) mixes sample, last silica gel H chromatography column, with sherwood oil: ethyl acetate=20: 1-1: 1 solvent systems gradient elution, collect sherwood oil: the elutriant of ethyl acetate=15: 1, concentrating under reduced pressure, mix sample with silica gel H, last silica gel H chromatography column, with sherwood oil: ethyl acetate=30: 1 normal pressure constant speed wash-outs obtains same Rhizoma Petasitis tricholobi lactone, Bakkenolide III a and Rhizoma Petasitis tricholobi lactone-IV a successively; Collect sherwood oil: the elutriant of ethyl acetate=8: 1, concentrating under reduced pressure is mixed sample with silica gel H, last silica gel H chromatography column, with sherwood oil: degree such as ethyl acetate=20: 1 normal pressures constant speed wash-out obtains Rhizoma Petasitis tricholobi lactone VII a, Rhizoma Petasitis tricholobi lactone-I a and Bakkenolide B successively; Collect sherwood oil: the elutriant of ethyl acetate=5: 1, concentrating under reduced pressure is mixed sample with silica gel H, last silica gel H chromatography column, sherwood oil: ethyl acetate=10: 1 normal pressure constant speed wash-outs, successively must Bakkenolide D and Bakkenolide L.Wherein, Rhizoma Petasitis tricholobi lactone VII a is a new compound.
Through experimentation on animals; the present invention slaps leaf Rhizome of Japanese Butterbur extract or compound Rhizoma Petasitis tricholobi lactone VII a and can significantly improve creatine kinase content in the cerebral tissue, significantly reduce the content of lactic acid in the cerebral tissue; can resist myocardial ischemia; inhibited to tumour cell; focal brain ischemia-reperfusion injury had provide protection; acute myocardial ischemia due to the coronary artery ligation had provide protection; can obviously improve the learning and memory function of dementia rats; allergic rhinitis had preventive and therapeutic effect, to LTB in people's whole blood
4Generation the obvious suppression effect is arranged, guinea-pig isolated tracheal smooth muscle due to the histamine shunk the obvious suppression effect, therefore can be used for preparing the medicine of control cardiovascular and cerebrovascular diseases, allergic rhinitis, tumour, senile dementia, dysthymia disorders, histamine and/or leukotrienes composition relative disease.
The present invention slaps leaf Rhizome of Japanese Butterbur extract and compound Rhizoma Petasitis tricholobi lactone VII a preparation method is simple, with low cost, the present invention provides a kind of new medicament sources for control cardiovascular and cerebrovascular diseases, allergic rhinitis, tumour, senile dementia, dysthymia disorders, histamine and/or leukotrienes composition relative disease.
Embodiment
Now in conjunction with the embodiments the present invention is described in detail.
Embodiment 1. preparation palm leaf Rhizome of Japanese Butterbur extracts.
Used palm leaf Rhizome of Japanese Butterbur is collected in northeast.
(1) the dry rhizome 50kg of palm sawfly bucket dried vegetable, pulverize back with the diacolation extraction routinely of 50% aqueous ethanolic solution, extracting solution is evaporated to the medicinal extract that does not have the alcohol flavor, to be suspended to volume be 50L to water then, use 50L petroleum ether extraction 3 times more respectively, merge petroleum ether extraction liquid, concentrating under reduced pressure must be slapped leaf Rhizome of Japanese Butterbur extract 9.6kg.
(2) the dry rhizome 50kg of palm sawfly bucket dried vegetable, pulverize back with the diacolation extraction routinely of 95% aqueous ethanolic solution, extracting solution is evaporated to the medicinal extract that does not have the alcohol flavor, to be suspended to volume be 50L to water then, use 50L petroleum ether extraction 3 times more respectively, merge petroleum ether extraction liquid, concentrating under reduced pressure must be slapped leaf Rhizome of Japanese Butterbur extract 2.4kg.
(3) the dry rhizome 50kg of palm sawfly bucket dried vegetable, pulverize back with the diacolation extraction routinely of 70% aqueous ethanolic solution, extracting solution is evaporated to the medicinal extract that does not have the alcohol flavor, to be suspended to volume be 50L to water then, use 50L petroleum ether extraction 3 times more respectively, merge petroleum ether extraction liquid, concentrating under reduced pressure must be slapped leaf Rhizome of Japanese Butterbur extract 5.5kg.
Embodiment 2. preparation Rhizoma Petasitis tricholobi lactone VII a
Palm leaf Rhizome of Japanese Butterbur extract 7g silica gel H (60 types with (1) preparation among the embodiment 1, Qingdao Haiyang Chemical Industry Group Corp., mix sample down together), last silica gel H chromatography column, with sherwood oil: ethyl acetate=20: 1-1: 1 solvent systems gradient elution, collect sherwood oil: the elutriant of ethyl acetate=15: 1, concentrating under reduced pressure, mix sample with silica gel H, last silica gel H chromatography column, with sherwood oil: ethyl acetate=30: 1 normal pressure constant speed wash-outs obtains successively with Rhizoma Petasitis tricholobi lactone 150mg, Bakkenolide III a30mg, Rhizoma Petasitis tricholobi lactone-IV a 55mg; Collect sherwood oil: the elutriant of ethyl acetate=8: 1, concentrating under reduced pressure, mix sample with silica gel H, last silica gel H chromatography column, with sherwood oil: degree such as ethyl acetate=20: 1 normal pressures constant speed wash-out, obtain Rhizoma Petasitis tricholobi lactone VII a 165mg successively, Rhizoma Petasitis tricholobi lactone-I a 256mg, Bakkenolide B 600mg; Collect sherwood oil: the elutriant of ethyl acetate=5: 1, concentrating under reduced pressure is mixed sample with silica gel H, last silica gel H chromatography column, sherwood oil: ethyl acetate=10: 1 normal pressure constant speed wash-outs, successively must Bakkenolide D 30mg and Bakkenolide L 688mg.
Structure is identified:
Process
1H and
13C-NMR composes and mass spectroscopy, and contrasts with document, identifies that obtaining the same Rhizoma Petasitis tricholobi lactone of known lactone compound (sees Siegenthaler P for details, Neuenschwander M.Pharm Acta Helv 1996,71:345-353), Bakkenolide III a (sees Wang YL for details, Li RP, Guo ML, Zhang G, Zhang N, Ma YL.Planta Med, 2009,75:230-235), Rhizoma Petasitis tricholobi lactone-IV a (sees Wang YL for details, Li RP, Guo ML, Zhang G, Zhang N, Ma YL.Planta Med, 2009,75:230-235), Rhizoma Petasitis tricholobi lactone-I a (sees Wang YL for details, Li RP, Guo ML, Zhang G, Zhang N, Ma YL.PlantaMed, 2009,75:230-235), Bakkenolide B (sees Cheng Jiekai for details, Chinese Pharmaceutical Journal, 1999,34:734-736), Bakkenolide D (sees Shirahata K for details, Tetrahedron Lett, 1968,16:1993), Bakkenolide L (sees Wu TS for details, KaoMS, Wu PL, Lin FW, Shi LS, Liou MJ, Li CY.Chem.Pharm.Bull., 1999,47:375-382) and new compound Rhizoma Petasitis tricholobi lactone VII a.
New compound Rhizoma Petasitis tricholobi lactone VII a is a white powder, and its structure is identified: ESI-MS:m/z 389[M-H]
-, determining that compound molecular weight is 390, HR-MS shows that molecular formula is: C
22H
30O
6
From
13C-NMR spectrum and DEPT contain 22 C signals in this compound in composing as can be known, comprise 7 season C, 5-CH, 5-CH
2With 5 CH
3Can further belong to the hydrocarbon of this compound according to the HMQC spectrum.Drawing this compound from above information is sesquiterpenoids.
Among the HMBC, the C of the H of δ=5.76 and δ=169.9,147.7 is relevant, illustrates that a side chain is connected on 9 the C; The C of the H of δ=5.09 and δ=165.5,80.8 is relevant, and this CH is described
2Link to each other with the ketone carbonyl, the H of δ=3.5 and δ=141.1,145.0,195.5,23.8 C is relevant, illustrates that another side chain is connected on 3 the C.Coupling correlationship between all the other each signals is as shown in table 1.
Comprehensive above the analysis determined the complete structure of this compound, called after Rhizoma Petasitis tricholobi lactone VII a, and its structural formula is:
The 1H of table 1. Rhizoma Petasitis tricholobi lactone VII a,
13C-NMR data (400MHz, δ, CDCl
3)
Pharmacology, effect experiment
It is basic identical that the present invention slaps pharmacology or the pharmacodynamic experiment method of leaf Rhizome of Japanese Butterbur extract and Rhizoma Petasitis tricholobi lactone VII a, and existing details are as follows:
One, to the preventive and therapeutic effect of mouse global brain ischemia
The present invention is with mouse broken end global brain ischemia model research palm leaf Rhizome of Japanese Butterbur extract and the Rhizoma Petasitis tricholobi lactone VII a preventive and therapeutic effect to cerebral ischemia.
1, experiment material and method
Laboratory animal provides (down together) by Second Military Medical University, PLA's animal center; Testing used palm leaf Rhizome of Japanese Butterbur extract is the extract of (1) preparation among the embodiment 1, and Rhizoma Petasitis tricholobi lactone VII a is by embodiment 2 preparations (down together).
40 of ICR mouse, body weight: 18～22g, male and female half and half, be divided into 4 groups at random, promptly slap leaf Rhizome of Japanese Butterbur extract group, Rhizoma Petasitis tricholobi lactone VII a group, blank group and positive controls, 10 every group, the medicine wiring solution-forming, solvent is 0.5% the tragakanta aqueous solution, the difference gastric infusion, and positive control drug is a nimodipine, the blank group is irritated stomach equal-volume solvent, dosage sees Table 2～table 4 respectively, sacrificed by decapitation mouse after 5 days, record broken end back mouse mouth breathing number of times and breathing time.Get the about 100mg of right hemisphere, use physiological saline homogenate, measure creatine kinase, lactic acid content in the tissue homogenate.Used creatine kinase, lactic acid content mensuration test kit build up bio-engineering research by Nanjing and are produced, and using method sees the description of product for details.
2, experimental result
Measurement data is with mean ± standard deviation
Expression, each group compares with the blank group respectively, adopts bilateral two sample t check carrying out statistical study.The results are shown in Table 2～table 4.
Table 2. mouse broken end back mouth breathing time length cartogram
Compare * P＜0.05 with the blank group
Creatine kinase content cartogram in the table 3. mouse brain tissue homogenate (x ± SD)
Compare * P＜0.05, * * P＜0.01 with the blank group
Lactic acid content cartogram in the table 4. mouse brain tissue homogenate (x ± SD)
Compare * * P＜0.01 with the blank group
By table 2～table 4 as seen, palm leaf Rhizome of Japanese Butterbur extract or Rhizoma Petasitis tricholobi lactone VII a can break end the back mouth breathing time length (P＜0.05) by the significant prolongation mouse, and can significantly improve the content (P＜0.01) that creatine kinase content in the cerebral tissue (P＜0.05) also significantly reduces lactic acid in the cerebral tissue.Illustrate that the present invention slaps leaf Rhizome of Japanese Butterbur extract or Rhizoma Petasitis tricholobi lactone VII a has significant curative effect to the mouse global brain ischemia.
Two, to the provide protection of focal brain ischemia-reperfusion injury in rats
1, experiment material and method
The tragakanta aqueous solution with 0.5% is solvent, preparation palm leaf Rhizome of Japanese Butterbur extract and Rhizoma Petasitis tricholobi lactone VII a solution, the positive contrast medicine of Nimoldipine.
Get 50 of male SD rats, body weight 180～230g is divided into 5 groups at random, every group 10, the 1st group is sham operated rats, the 2nd group of negative control group, that is group of solvents, injection equal-volume solvent, the 3rd group is palm leaf Rhizome of Japanese Butterbur extract group, by the body weight administration, dosage is 40mg/kg, and the 4th group is Rhizoma Petasitis tricholobi lactone VII a group, and dosage is 10mg/kg, the 5th group is the Nimoldipine group, dosage 2mg/kg.Administration time is an abdominal injection immediately behind the ischemic, and the administration volume is 1ml.
With reference to the line bolt model of Longa EZ report prepare cerebral ischemia re-pouring injured model (Longa EZ, et al:Reversablemiddle cerebral artery occlusion without craniectomy in rat.Stroke, 1989,20:84).Ischemic poured into laggard capable study of behaviour scoring in 24 hours in 2 hours again, broken end is got brain, TTC dyeing, leaving and taking sample carries out cerebral infarct volume and measures and (to see the Zhang Juntian chief editor for details: the modern pharmacology experimental methodology, the 1241st page, combined publication society of China Concord Medical Science University of Beijing Medical University, October in 1998 the 1st edition).
All data all adopt
The t check is adopted in expression, statistical analysis.
2, experimental result the results are shown in Table 5.
Table 5 palm leaf Rhizome of Japanese Butterbur extract and Rhizoma Petasitis tricholobi lactone VII a to the provide protection of focal brain ischemia-reperfusion injury in rats (n=10,
)
*P＜0.05,
*Compare with group of solvents P＜0.01
As can be seen from Table 5, palm leaf Rhizome of Japanese Butterbur extract or Rhizoma Petasitis tricholobi lactone VII a can significantly reduce the rat behavior scoring, dwindle brain infarction area, alleviate ischemic side brain hemisphere oedema degree.Show that palm leaf Rhizome of Japanese Butterbur extract or Rhizoma Petasitis tricholobi lactone VII a all have strong provide protection to focal brain ischemia-reperfusion injury in rats.
Three, to Racemic isoproterenol (ISO) institute to the therapeutic action of rat heart muscle ischemic
1, experiment material and method
32 of Healthy female SD rats, body weight 180～230g is divided into 4 groups at random, is respectively blank group, model control group, palm leaf Rhizome of Japanese Butterbur extract group and Rhizoma Petasitis tricholobi lactone VII a group.Blank group and model control group are pressed body weight intraperitoneal injection of saline 2ml/kg in advance respectively, and palm leaf Rhizome of Japanese Butterbur extract group and Rhizoma Petasitis tricholobi lactone VII a group be abdominal injection 2ml/kg respectively, and every day 1 time, dosage sees Table 6, successive administration three days.0.5h is by body weight subcutaneous injection ISO 0.15ml/100g after model control group, palm leaf Rhizome of Japanese Butterbur extract group and Rhizoma Petasitis tricholobi lactone VII a group second day and the administration in the 3rd day, the physiological saline of capacity such as blank group injection, write down respectively before the last administration and the 2nd injection ISO after the electrocardiogram(ECG (standard I I leads) of 30min, measure the J point value, respectively organize the absolute value of rat J point displacement behind the calculating injection ISO.The rat aorta blood sampling, centrifugal separation plasma behind the anticoagulant heparin, LDH activity unit in the spectrophotometry blood plasma.Each administration group statistics and model group are relatively carried out the t check.The serum lactic dehydrogenase calculation formula is as follows:
2, experimental result the results are shown in Table 6, table 7.
Table 6, the palm leaf Rhizome of Japanese Butterbur extract and Rhizoma Petasitis tricholobi lactone VII a to the influence of rat heart muscle ischemic J point displacement due to the ISO (n=8,
)
*P＜0.05,
*Compare with model control group p＜0.01
△P＜0.05,
△ △Compare with the blank group p＜0.01
Table 7, the palm leaf Rhizome of Japanese Butterbur extract and Rhizoma Petasitis tricholobi lactone VII a to the influence of ISO rat LDH activity unit (
N=8)
*P＜0.05,
*Compare with model control group p＜0.01
△P＜0.05,
△ △Compare with the blank group p＜0.01
Experimental result shows, behind rat skin lower injection ISO, the model control group animal displacement of J point occurs and increases, the significance myocardial ischemia that the LDH unit of activity increases in the blood plasma changes, palm leaf Rhizome of Japanese Butterbur extract or Rhizoma Petasitis tricholobi lactone VII a intraperitoneal injection can significance reduce LDH level in J point displacement and the blood plasma, compare with the blank group, palm leaf Rhizome of Japanese Butterbur extract or Rhizoma Petasitis tricholobi lactone VII a group all present function of resisting myocardial ischemia.
This shows that palm leaf Rhizome of Japanese Butterbur extract or Rhizoma Petasitis tricholobi lactone VII a have significant inhibitory effect to the rat heart muscle ischemic that ISO causes, and can alleviate the damage of ischemic myocardium, therefore myocardial ischemia is had obvious provide protection.Four, to the provide protection of rat coronary artery caused by ligature acute myocardial ischemia
1, experiment material and method
Get 40 of healthy SD rats about body weight 200g, male and female half and half are divided into 4 groups at random, by the body weight administration, and model control group, physiological saline 10ml/kg; Palm leaf Rhizome of Japanese Butterbur extract group, dosage is 40mg/kg; Rhizoma Petasitis tricholobi lactone VII a group, dosage is 10mg/kg; Positive controls, the contrast medicine is that beta receptor is picked up anti-agent Proprasylyte, dosage is 5mg/kg.Gastric infusion, every day 1 time, continuous 2 days.
1h after the last administration, 12% Chloral Hydrate 3ml/kg anaesthetizes by the body weight abdominal injection, the record normal ECG, chest unhairing, sterilization, along the about 2cm of left mid-clavicular line longitudinal incision skin, separate the flesh layer in the 4th or the 5th intercostal passivity, open the thoracic cavity, cut off pericardium, the light right side thorax of pressing is extruded heart, behind ligation arteria coroaria sinistra in coronary vein place between right side circular cone and the left auricle of heart, heart is put back to the thoracic cavity, fully discharge air in the thoracic cavity, sew up and close thoracic cavity, respirator assisted respiartion, frequency 20 times/minute, Tidal volume 5ml.Respectively at after the ligation at once, 1h, 24h, 48h trace one section electrocardiogram(ECG, and in 24h, 48h gastric infusion respectively.
Behind coronary ligation 50h, rat is anaesthetized once more, aorta abdominalis blood sampling, anticoagulant heparin, 3000 collect blood after leaving heart 10min, measure LDH, and after getting blood, take out heart rapidly, use normal saline flushing, remove blood stains, reject non-cardiac muscular tissues such as blood vessel, fat, inhale the branch that anhydrates, claim weight in wet base whole-heartedly with thieving paper.Along coronary sulcus excision atrium, stay ventricle, weigh, along coronary sulcus from the apex of the heart to heart base portion 4 of parallel myocardium sheets with ventricular muscles crosscut Cheng Houyue 0.1cm, clean with normal saline flushing, myocardium sheet is placed in 0.1% the N-BT solution, the 15min that under 37 ℃ of water bath condition, dyes, unnecessary dyestuff is removed in water flushing immediately after the dyeing.Infarcted region is not painted, and non-infarcted region is dyed blueness by N-BT solution.Cut off infarcted myocardium, claim weight in wet base, account for whole-heartedly the per-cent of weight in wet base with the infarcted region weight in wet base and represent myocardial infarct size.Each administration group statistics and model control group are relatively carried out the t check.
2, experimental result the results are shown in Table 8, table 9.
Table 8, the palm leaf Rhizome of Japanese Butterbur extract and Rhizoma Petasitis tricholobi lactone VII a to coronary artery ligation cause the active influence of Model Rats with Acute Myocardial Ischemia LDH (
N=10, U/L)
*Compare with model control group p＜0.01
Table 9, the palm leaf Rhizome of Japanese Butterbur extract and Rhizoma Petasitis tricholobi lactone VII a to coronary artery ligation cause Model Rats with Acute Myocardial Ischemia ischemic scope influence (
N=10, %)
*Compare with model control group p＜0.01
Experimental result shows, the displacement of rats underwent coronary artery ligation postoperative J point raises rapidly and peaked in 1 hour after ligation, ligation after 50 hours in the model control group animal plasma LDH activity highly significant rising is arranged, the myocardial ischemia area also significantly increases.Palm leaf Rhizome of Japanese Butterbur extract or Rhizoma Petasitis tricholobi lactone VII a can significantly reduce LDH activity (P＜0.01) and myocardial infarct size (P＜0.01).
This shows that palm leaf Rhizome of Japanese Butterbur extract or Rhizoma Petasitis tricholobi lactone VII a gastric infusion have significant protective effect to the rat heart muscle ischemic that coronary ligation causes, can significantly reduce the tissue necrosis that myocardial ischemia causes, ischemia group is woven with significant protective effect.
Five, to the effect of cavy Prevention and Cure of Allergic Rhinitis
The present invention utilizes oralbumin inductive allergic rhinitis cavy Research of Animal Model for Study palm leaf Rhizome of Japanese Butterbur extract and Rhizoma Petasitis tricholobi lactone VII a to the Prevention and Cure of Allergic Rhinitis effect.
1, experiment material and method
24 cavys, the first day is respectively injected 0.15ml antigen adjuvant suspension in 4 sufficient meat pads (every milliliter contains Protalbinic acid 1mg, bordetella pertussis 1 * 10
10Individual and aluminium hydroxide 2mg) carries out first sensitization.After 5 days, the normal saline solution reinforcement sensitization that 1ml contains the 0.5mg Protalbinic acid is injected in 10 sites of subcutaneous branch altogether at the back.The 14th～28 day every day in the bilateral nasal cavity with micropipet instillation OVA normal saline solution (1mg/ml, every nostril 10ul) carrying out local antigen attacks, and count immediately and scratch nose number of times observed and recorded cavy nasal mucus in the cavy 30min, the sneeze symptom, judgement criteria sees Table 10.Behind 15-20 days continuous counters, sensitization success cavy was divided into 2 groups according to scratching the nose number of times in the 20th day, be subjected to the reagent thing on the 21st～27 day, attacked at antigen by reagent thing and control sample and irritated stomach in preceding 1 hour and give, and the record cavy is scratched the nose number of times.Cavy was put to death in the 28th day.
2, experimental result
Table 10 cavy sniffle observation caliber
Observe above symptom after administration in 30 minutes and 2 hours, with superposition method record total points, total points surpasses 5 fens and is the modeling success, and observations sees Table 11.
Table 11. sniffle cavy observations
| Scratch the nose number of times | Quantity (N) | The rhiocnesmus mark | Clear tears mark | The sneeze mark | The mark remarks |
| 50-70 | 3 | 2 | 1 | 2 | 5 |
| 30-50 | 13 | 3 | 1 | 2 | 6 |
| 20-30 | 8 | 2 | 1 | 2 | 5 |
| Substantially do not grab | 0 | 0 | 0 | 0 | 0 |
By experimental result as seen, after sensitization is attacked, cavy grabs the nose number of times and significantly rises, and with clear tears and sneeze symptom, strong reaction, the 21st～27 day, the back was with egg white albumen attack in vain after the administration, and palm leaf Rhizome of Japanese Butterbur extract and Rhizoma Petasitis tricholobi lactone VII a and negative control group have the significance meaning more statistically; But after administration for some time, palm leaf Rhizome of Japanese Butterbur extract and Rhizoma Petasitis tricholobi lactone VII a and negative control group all show scratches nose number of times ascendant trend slightly, and each is organized cavy and scratches nose number of times statistics, sees Table 12.
After table 12 administration, cavy grabs nose number of times statistics
Compare * P＜0.05 with model control group
Six, to the extracorporeal anti-tumor function of tumour cell
1, experiment material and method
The MTT method is measured
Principle: MTT (3-(4,5-dimethyl-2-thiazoyl)-2,5-diphenyl-tetrazolium bromide) [3-(4,5-dimethylthiazole-2)-2,5. hexichol tetrazole bromine salt], be a kind of yellow dyes that can accept H+.In the viable cell mitochondrial respiratory chain, succinodehydrogenase and cytochrome C can make the tetrazole ring opening of MTT, generate hepatic formazan crystallization.And do not contain this desaturase in the dead cell.Formazan crystalline growing amount is directly proportional with viable count, and this crystalline DMSO solution has maximum absorption band at the 570nm place, so, can estimate the influence of medicine on cell proliferation by detecting formazan crystalline amount.The concrete operations step is as follows:
(1) tumour cell of taking the logarithm vegetative period (seeing Table 13) transfers to every milliliter 5 * 10 with cell density with cell culture fluid DMEM
4Individual cell is added in the 96 porocyte culture plates, every hole 100 μ l.Palm leaf Rhizome of Japanese Butterbur extract group concentration is 5 μ μ/ml, and Rhizoma Petasitis tricholobi lactone VII a group concentration is 5 μ g/ml, establishes 6 parallel samples for every group, and every hole adds 100 μ l, establishes the blank group simultaneously, and every hole adds cell culture fluid DMEM 100 μ l, in 37 ℃, 5%CO
2Constant incubator in cultivate 12h.
(2) every hole adding concentration is the MTT liquid 30 μ l of 5mg/ml, in 37 ℃ of 5%CO
2Incubator in continue to cultivate 4h.
(3) supernatant liquor is removed in suction, and every hole adds DMSO150 μ l, and vibration is 15 minutes on micro oscillator, and after dissolving fully to crystallization, microplate reader is measured the light absorption value (OD value) at 570nm place, every hole.
(4) get IR%=(OD blank-OD sample)/OD blank * 100% calculating by formula of the average OD value in three holes and slap the inhibiting rate (IR%) of leaf Rhizome of Japanese Butterbur extract or Rhizoma Petasitis tricholobi lactone VII a on cell proliferation.
2, experimental result
Table 13MTT experiment given the test agent is to the tumor cell proliferation inhibition rate
The MTT experiment shows that palm leaf Rhizome of Japanese Butterbur extract or Rhizoma Petasitis tricholobi lactone VII a all have remarkable restraining effect to HepG2, MCF-7, A549 or Hela.
Seven, to the antitumor action of S180 sarcoma in the ICR mouse body
1, experiment material and method
50 of ICR mouse, body weight: 18～22g is divided into 5 groups (seeing Table 14), S180 cell counting 1.6 * 10 at random
7Individual/ml, physiological saline is diluted to 6 * 10
5Individual/ml, every mouse neck place's subcutaneous vaccination 0.2ml then, in inoculation administration in second day, dosage sees Table 14, positive controls is an endoxan, inoculating the 7th day left and right sides mouse back has projection, continues administration, the 15th day, eye socket is got blood, take off neck and put to death, peel off tumour, spleen and thymus gland, weigh.
2, experimental result
(1) blood preparation: measure WBC by the Changhai hospital laboratory, HGB, PLT the results are shown in Table 14.
WBC in each experimental mice blood of table 14, HGB, PLT measurement result
Compare with model control group: * * P＜0.01, * P＜0.05, WBC, HGB, PLT are respectively white corpuscle, red corpuscle, oxyphorase, thrombocyte.
(2) tumor weight and thymus gland, spleen data see Table 15.
Each experimental mice tumour spleen of table 15, spleen and thymus gland result
Compare with model control group: * * P＜0.01, * P＜0.05
By table 15 as seen, palm leaf Rhizome of Japanese Butterbur extract or Rhizoma Petasitis tricholobi lactone VII a have certain anti-tumor activity, and endoxan is very big to the spleen influence of ICR mouse.
Eight, to the therapeutic action of senile dementia
It is exactly damage in learning and memory that the clinical symptom of senile dementia one of mainly shows.Learning and memory is one of critical function of brain, the intellectual level of its reaction brain.And what can weigh the ability of its learning and memory by the mistake made in the animal behavior.Scopolamine inductive Model of Dementia in Rats is exactly to utilize this principle to study the influence of medicine to animal intelligence.We adopt MORRIS water maze method and keep away two kinds of methods of dark experiment, use Scopolamine learning memory disorder model, observe palm leaf Rhizome of Japanese Butterbur extract and the Rhizoma Petasitis tricholobi lactone VII a influence to learning and memory in rats.
1, experiment material and method
The MORRIS water maze is the cylindrical pond of a diameter 120cm, high 50cm, with the center of circle at the bottom of the pond is that the center is divided into four quadrants with the labyrinth, the transparent column type synthetic glass platform of one diameter 12cm, high 30cm is placed a certain quadrant center, the top, labyrinth is settled and is had the video camera that is connected with computer system, but synchronous recording rat motor track and seek platform latent period etc., object of reference and position of platform remain unchanged outside the experimental session labyrinth.Select 4 place of entry at pool wall during experiment, distance is not less than 25cm between adjacent two place of entry, adds tap water in the pond, dyes opaque oyster white with milk powder, and the water surface is approximately higher than flat surface 2cm, and water temperature remains on about 25 ℃.70 * 20 * 30cm rat is shuttled back and forth and keeps away camera bellows two chambers of equal-sized light and shade are arranged, and the bottom, chamber is equipped with the steel grid, and darkroom steel grid can lead to the 38V alternating-current as incentive condition, has the round hole of a diameter 10cm that two Room are continuous on the central dividing plate.Shuttle box links to each other with computer, can write down rat automatically and enter time in darkroom and number of times etc.
MORRIS water maze laboratory: 50 of SD rats, body weight 180～230g, be divided into 5 groups at random, be respectively the blank group, model control group, positive controls, palm leaf Rhizome of Japanese Butterbur extract group and Rhizoma Petasitis tricholobi lactone VII a group, drug solvent is the aqueous suspension of 0.5% tragakanta, positive control drug is Hup A, blank group and model control group are irritated stomach with the aqueous suspension of 0.5% tragakanta, positive controls is irritated stomach Hup A 4mg/kg, palm leaf Rhizome of Japanese Butterbur extract group is pressed the 40mg/kg gastric infusion, Rhizoma Petasitis tricholobi lactone VII a group is pressed the 10mg/kg gastric infusion, every day 2 times, totally 8 days.After the every day of the administration for the first time 1 hour, begin to carry out the training of MORRIS water maze.Experiment allows rat free swimming 2 minutes to adapt to surrounding environment the day before yesterday, since first day, train every day 4 times, select a place of entry at random at every turn, rat is put into water towards pool wall, observe and write down route map and the required time (latent period) that rat sought and climbed up platform.4 trained rat, need it is caused platform if rat was not found platform in 120 seconds respectively from four different people's water spot people water.At this moment be designated as 120 seconds latent period, each training 60 seconds at interval, continuous 7 days.After the last administration in the 8th day 1 hour, blank group rats by intraperitoneal injection physiological saline 1ml/kg, all the other respectively organize rats by intraperitoneal injection scopolamine hydrobromide 2mg/kg.Every rat injection end back 25min is fixed into water spot by one and is dropped in the water towards pool wall, observes and write down the time (latent period) that it is sought platform route and finds platform.
Keep away dark experiment: 50 of SD rats, body weight 180～230g, the grouping and dosage with above-mentioned MORRIS water maze laboratory, gastric infusion, every day 2 times, totally 8 days.1 hour begins training after the last administration in the 8th day, except that the blank group, and every group of rat 25min abdominal injection scopolamine hydrobromide 2.0mg/kg before training, the blank group is physiological saline 1.0ml/kg.During training rat is put into case endoadaptation 3min earlier, connect power supply then, animal back is placed to the hole keep away the bright chamber of camera bellows, observe animal and enter the total degree (number of times of making a mistake) that enters the darkroom in time (latent period) in darkroom and the 5min first.Repeated to keep away dark experimental implementation on the 9th day and write down latent period and make mistakes the number as the memory indexs.
2, experimental result
In adaptation training, all animal activities are normal; In initial training, rat mainly seeks platform with marginal mode (D) and random mode (C), and along with training increases, except that the model control group animal still has most of the maintenance this search strategy, all the other each groups all are mainly orthoscopic (A) and trend formula (B).As can be seen from Table 16, compare with model control group, obviously shorten the latent period that the rat of palm leaf Rhizome of Japanese Butterbur extract group or Rhizoma Petasitis tricholobi lactone VII a group seeks platform, and difference has statistical significance.
Table 16 is respectively organized the latent period that rat seeks platform
* P＜0.05, * * P＜0.01 and model control group comparison
In keeping away dark experiment, the rat of the palm leaf Rhizome of Japanese Butterbur extract group and the Rhizoma Petasitis tricholobi lactone VII a group digital display work of making mistakes is lower than model control group, and latent period is prolongation greatly then.The results are shown in Table 17.
Table 17 is respectively organized rat and is made a mistake number of times and latent period relatively
* P＜0.05, * * P＜0.01, * * * P＜0.001 and model control group comparison
The above results shows that palm leaf Rhizome of Japanese Butterbur extract or Rhizoma Petasitis tricholobi lactone VII a can obviously improve the learning and memory function of dementia rats, therefore senile dementia is had certain prevention and therapeutic action.
Nine, to the dysthymia disorders effect
Mouse forced swimming experiment (mouse forced swimming test) is classical depressed animal model, slaps leaf Rhizome of Japanese Butterbur extract and the Rhizoma Petasitis tricholobi lactone VII a preventive and therapeutic effect to the experimental animal models dysthymia disorders so investigate with it.
1, experiment material and method
Method by Zhang Juntian chief editor " modern pharmacology experimental technique " (first volume) the 104th page is carried out.With 40 of mouse, body weight: 18～22g is divided into 4 groups at random, every group 10, be respectively blank group, positive controls, palm leaf Rhizome of Japanese Butterbur extract group and Rhizoma Petasitis tricholobi lactone VII a group, put into 10 centimetres of the depth of waters, (20 centimetres high for the graduated cylinder that water temperature is 25 ± 1 ℃, 14 cm diameters) swimming in makes it produce the feared state of mind, takes out after 5 minutes, 32 ℃ down with after hairy the doing, give the laboratory animal gastric infusion in 24 hours respectively, the blank group gives isopyknic physiological saline; Positive controls gives the medicine imipramine, and dosage is 20mg/Kg; Palm leaf Rhizome of Japanese Butterbur extract group, dosage is: 60mg/Kg; Rhizoma Petasitis tricholobi lactone VII a group, dosage is: 15mg/Kg.Again mouse is put into above-mentioned environment, measure mouse and in 6 minutes back 4 minutes, keeps the motionless time of swimming, as the standard of judging the medicine antidepressant effect, test triplicate with the shortening of dead time.
2, experimental result
Table 18 pair mouse forced swimming is tested the result that influences of behavior
Compare with the blank group
*P＜0.05, * * P＜0.01
As can be seen from Table 18, palm leaf Rhizome of Japanese Butterbur extract or Rhizoma Petasitis tricholobi lactone VII a all can significantly reduce the mouse forced swimming dead time, show that palm leaf Rhizome of Japanese Butterbur extract or Rhizoma Petasitis tricholobi lactone VII a all have antidepressant activity, therefore can be used to prepare the medicine of preventing and treating dysthymia disorders.
Ten, to leukotriene B in people's whole blood
4(LTB
4) restraining effect that produces
The leukotriene B that this test determination palm leaf Rhizome of Japanese Butterbur extract and Rhizoma Petasitis tricholobi lactone VII a inhibition are excited by Calcium ionophore
4Generation.Because LTB
4Generation be that activation by the 5-lipoxidase mediates, so this tests the ability that measurable sample suppresses the 5-lipoxidase.
1, experiment material and method
Experiment is divided into 3 groups, palm leaf Rhizome of Japanese Butterbur extract group, Rhizoma Petasitis tricholobi lactone VII a group and blank group.To slap leaf Rhizome of Japanese Butterbur extract and Rhizoma Petasitis tricholobi lactone VII a is dissolved in DMSO solution respectively, the concentration of palm leaf Rhizome of Japanese Butterbur extract is 15 μ g/ml, and the concentration of Rhizoma Petasitis tricholobi lactone VII a is 5 μ g/ml.Extract normal people volunteer's blood, anticoagulant heparin, in being the polypropylene tube of 1.5ml, volume adds 1ml blood respectively, by above-mentioned grouping, palm leaf Rhizome of Japanese Butterbur extract group adds the Rhizome of Japanese Butterbur extract solution of 5 μ l, Rhizoma Petasitis tricholobi lactone VII a group adds the Rhizoma Petasitis tricholobi lactone VII a solution of 5 μ l, and the blank group adds 5 μ l DMSO solution.To slap leaf Rhizome of Japanese Butterbur extract group then, the polypropylene tube of Rhizoma Petasitis tricholobi lactone VII a group and blank group was placed in 37 ℃ the water-bath cultivation 15 minutes, the calcium ion carrier A 23187 (ultimate density is 50 μ M) that in each pipe, adds 5 μ l again, behind the mixing, relay in the backwater bath and placed 30 minutes, with centrifugal 10 minutes of rotating speed 2500rpm low temperature (4 ℃), respectively 50 μ l supernatant liquors are transferred to and cooled off in advance well, contain in the Eppendorf tube of enzyme immunity test (EIA) damping fluid of 950 μ l, use EIA test kit (Cayman ChemicalCo., the U.S.) to measure LTB then
4To slap leaf Rhizome of Japanese Butterbur extract group, Rhizoma Petasitis tricholobi lactone VII a group and blank group and compare, and calculate in view of the above and produce LTB
4The inhibition percentage and its IC
50
2, experimental result
Palm leaf Rhizome of Japanese Butterbur extract and Rhizoma Petasitis tricholobi lactone VII a are to LTB
4IC
50Be respectively 1005nM and 913nM, show that palm leaf Rhizome of Japanese Butterbur extract or Rhizoma Petasitis tricholobi lactone VII a are to LTB
4The obvious suppression effect is all arranged.
11, the influence that guinea-pig isolated tracheal smooth muscle due to the histamine is shunk
1, experiment material and method
Reference literature (sees Hu Guosheng for details, Huang Xianju, DDPH uses pharmaceutical journal 1998 to the contemporary Chinese that influences that guinea-pig isolated tracheal smooth muscle due to the multiple medium shrinks, 15 (4): method 11-14), get 20 of male guinea pigs, body weight: 300-500g, be divided into 2 groups, shoot dead, separate immediately and cut tracheae, put into the plate that fills Ke-Heng Shi (Kerb-Henseleit) nutritive medium, the tracheae outside of belly (cartilage anchor ring) longitudinal incision, again in the crosscut of the interval of 2-3 cartilaginous ring, every section tracheae sheet is on its longitudinal incision place sews on, every 2-3 section tracheae sheet strings mutually, tracheae sheet sample, sample is suspended on 10ml bathes in the pipe, load 1.5g, 37 ℃ of insulations, logical oxygen (95%O in Ke-Heng Shi liquid
2+ 5%CO
2), the sample balance is half an hour approximately, and behind tension stability, beginning adds different concns (M) histamine in bathing pipe, and the tracheae sheet is shunk, record histamine different concns (10
-7, 10
-6, 10
-5, 10
-4) tonic effect (dosage adds up).The rinsing bath pipe makes tracheae sheet tension force be returned to original position (1g), after a period of stabilisation, adds given the test agent, after temperature is incubated 5min, adds different concns (M) histamine, and the tracheae sheet is shunk, record histamine different concns (10
-7, 10
-6, 10
-5, 10
-4) shrinking effect, whenever add given the test agent and observed 3 minutes.
With the maximum collapse effect is 100%, calculates contraction percentage.
Data statistics result mean+SD
Expression.
2, experimental result the results are shown in Table 19.
The influence that the different samples of table 19. shrink guinea-pig isolated tracheal smooth muscle
* all with on the same group compare before the administration P＜0.05 * * P＜0.01
From experimental result as can be seen, palm leaf Rhizome of Japanese Butterbur extract or Rhizoma Petasitis tricholobi lactone VII a all have restraining effect to the guinea-pig isolated trachea sheet contraction that histamine causes.
In sum, the present invention slaps leaf Rhizome of Japanese Butterbur extract or Rhizoma Petasitis tricholobi lactone VII a has the cerebral ischemia of control, myocardial ischemia, allergic rhinitis, tumour, dysmnesia isoreactivity, therefore can be used for preparing the medicine of control cardiovascular and cerebrovascular diseases, allergic rhinitis, tumour, senile dementia, dysthymia disorders, histamine and/or leukotrienes composition relative disease.
Claims (5)
1. one kind by the palm leaf Rhizome of Japanese Butterbur lactone compound Rhizoma Petasitis tricholobi lactone VII a that is separated in the palm leaf Rhizome of Japanese Butterbur extract, it is characterized in that chemical structure is as follows:
2. slap leaf Rhizome of Japanese Butterbur extract for one kind, the preparation method is as follows:
After will slapping the dry rhizome pulverizing of sawfly bucket dried vegetable, water or aqueous ethanolic solution diacolation routinely extract or soak and extract, and the extracting solution concentrating under reduced pressure becomes medicinal extract, uses petroleum ether extraction again behind the water suspendible then, with the extraction liquid concentrating under reduced pressure, must slap leaf Rhizome of Japanese Butterbur extract.
3. by the described palm leaf of claim 2 Rhizome of Japanese Butterbur extract, it is characterized in that its main active ingredient is Rhizoma Petasitis tricholobi lactone VII a, same Rhizoma Petasitis tricholobi lactone, Rhizoma Petasitis tricholobi lactone-I a, Bakkenolide III a, Bakkenolide-, Bakkenolide B, Bakkenolide D and Bakkenolide L.
4. the application of the described Rhizoma Petasitis tricholobi lactone VII of claim 1 a in the medicine of the relevant disease of preparation control cardiovascular and cerebrovascular diseases, allergic rhinitis, tumour, senile dementia, dysthymia disorders, histamine and/or leukotrienes composition.
5. claim 2 or the 3 described palm leaf Rhizome of Japanese Butterbur extracts application in the medicine of the relevant disease of preparation control cardiovascular and cerebrovascular diseases, allergic rhinitis, tumour, senile dementia, dysthymia disorders, histamine and/or leukotrienes composition.
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| CN102846484A (en) * | 2012-04-20 | 2013-01-02 | 朱丹 | Application of Petasites tatewakianus Kitam lactone in preparing anti-ultraviolet compositions |
| CN103999600A (en) * | 2014-03-06 | 2014-08-27 | 福建省农业区划研究所 | Method for manually reserving seeds of petasites tatewakianus kitam |
| CN105055403A (en) * | 2015-08-16 | 2015-11-18 | 南京华宽信息咨询中心 | Medicine for treating Alzheimer's disease, and application of medicine |
| CN106581113A (en) * | 2016-12-12 | 2017-04-26 | 中国人民解放军第二军医大学 | Application of petasites tricholobus franch extract in preparing medicines or health care foods for preventing and treating hypoxic diseases |
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| WO2004080473A1 (en) * | 2003-03-14 | 2004-09-23 | The Industry & Academic Cooperation In Chungnam National University (Iac) | Extract of petasites japonicus having neuroprotective effect and antioxidant activity |
| CN1844114A (en) * | 2006-04-27 | 2006-10-11 | 中国人民解放军第二军医大学 | Fukinanolide compounds and pharmaceutical application thereof |
| CN101297828A (en) * | 2008-06-17 | 2008-11-05 | 中国人民解放军第二军医大学 | Uses of Petasites tricholobus franch extract in medicament preparation for preventing and controlling cardiovascular and cerebrovascular diseases |
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| WO2004080473A1 (en) * | 2003-03-14 | 2004-09-23 | The Industry & Academic Cooperation In Chungnam National University (Iac) | Extract of petasites japonicus having neuroprotective effect and antioxidant activity |
| CN1844114A (en) * | 2006-04-27 | 2006-10-11 | 中国人民解放军第二军医大学 | Fukinanolide compounds and pharmaceutical application thereof |
| CN101297828A (en) * | 2008-06-17 | 2008-11-05 | 中国人民解放军第二军医大学 | Uses of Petasites tricholobus franch extract in medicament preparation for preventing and controlling cardiovascular and cerebrovascular diseases |
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|---|---|---|---|---|
| CN102846484A (en) * | 2012-04-20 | 2013-01-02 | 朱丹 | Application of Petasites tatewakianus Kitam lactone in preparing anti-ultraviolet compositions |
| CN102846484B (en) * | 2012-04-20 | 2014-04-16 | 朱丹 | Application of Petasites tatewakianus Kitam lactone in preparing anti-ultraviolet compositions |
| CN103999600A (en) * | 2014-03-06 | 2014-08-27 | 福建省农业区划研究所 | Method for manually reserving seeds of petasites tatewakianus kitam |
| CN103999600B (en) * | 2014-03-06 | 2015-11-18 | 福建省农业区划研究所 | A kind of palm leaf butterbur is manually reserved seed for planting method |
| CN105055403A (en) * | 2015-08-16 | 2015-11-18 | 南京华宽信息咨询中心 | Medicine for treating Alzheimer's disease, and application of medicine |
| CN106581113A (en) * | 2016-12-12 | 2017-04-26 | 中国人民解放军第二军医大学 | Application of petasites tricholobus franch extract in preparing medicines or health care foods for preventing and treating hypoxic diseases |
| CN106581113B (en) * | 2016-12-12 | 2020-02-21 | 中国人民解放军第二军医大学 | Application of petasites tricholobus franch extract in preparation of medicines or health-care food for preventing and treating anoxia diseases |
| CN108853103A (en) * | 2018-06-15 | 2018-11-23 | 王建涛 | A kind of antimycotic skin composition containing fukinanolide |
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