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Mushroom culture lecture
- 1. Mushroom Culture By Prof. Devilal N.Watakhere HOD of Botany RMGM Saoli
- 2. Table “Button” Mushroom Agaricus bisporus
- 3. Table “Button” Mushroom Agaricus bisporus • Eukaryote • Nuclei in Cells • Multicellular • Cell wall present • Heterotroph • Sessile • Mostly sexual reproduction • Kingdom Fungi
- 4. Table “Button” Mushroom Agaricus bisporus Kingdom Division Class Order Family Genus Species Fungi Basidiomycota (club fungi) Agaricomycetes Agaricales (gilled fungi) Agaricaceae Agaricus bisporus
- 5. Agaricus bisporus Native to North America, these common fungi are naturally found in manure heaps, garden waste and roadsides, as well as at the base of trees.
- 6. Agaricus bisporus The mushroom itself is just the “fruit” of a tangle of hyphae that are in the soil eating nutrients that are available.
- 7. Agaricus bisporus It’s only when the fungus attempts to breed and spread spores sexually that the mushroom grows up out of the soil.
- 8. Agaricus bisporus Like most members of the order Agaricales, table mushrooms have “gills”, or folds under the hood of the mushroom to allow spread of millions of new spores.
- 9. Varieties of Agaricus bisporus • Americans eat about 4 pounds (2 kg) of mushrooms each year. • In the US, over 700 million dollars are made in the mushroom industry each year.
- 16. Raising of pure culture done in the Laminar Air Flow Chamber to maintain aseptic condition.
- 17. Inoculation of fungal spores in culture media in test tube.
- 18. Keep this culture test tubes in incubator for two weeks.
- 19. 1.Master spawn culture medium is made from Wheat grains. 2. Boiled the Wheat grains and then spread on floor for drying.
- 20. Mixing of wheat grains with Gypsum and Calcium Carbonate
- 21. Packing of spawn medium in polythene bags
- 22. After packing keep bags in autoclave for maintaining septic condition in bags
- 23. Inoculation of mushroom spore in spawn media.
- 24. Master spawn ready kept in control room at 25 C
- 30. 1. Paddy straw mulching
- 31. CAN: Calcium Ammonium Nitrate, SSP: Single Super Phosphate MOP: Murate Of Potash, mix all. with urea and wheat bran
- 33. Covering of this mixture with cloth for two weeks.
- 34. Mixing of this mixture with paddy mulch.
- 35. Keep this mixture for 28 days.
- 37. Mix spawn of mushroom with compost,500 gm/Quintle.
- 38. Packing of spawn mixture in polythene bags.
- 39. Keep this bags in control room at 25 C temp. With 90% humidity, for 1-2 weeks.
- 41. 1.Mix the garden soil with farm yard manure (FYM). 2. Then spray this mixture with 4% formalin to maintain aseptic condition.
- 42. Keep it covered with sheet for one day.
- 44. Casing of soil on spawn bags.
- 45. Kept in control room at 90% humidity with 25 C temp.
- 46. 1.Mushroom start forming fruiting bodies in 10-15 days. 2. Period of mushroom growing is 40-50 days.
- 47. Harvesting is done with 7 days interval.
- 48. Same procedure done for Oyster mushroom culture.