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Legume Research- An International Journal, Volume 46 Issue 9: 1247-1252 (September 2023)
Background: Cercospora leaf spot (CLS) is one of the major diseases causing a major loss in production of mung bean of up to 60%. Although it is cultivated all round the world, its major contribution is attributed to the Asian continent especially the countries of Indian subcontinents which accounts for about 90% of the world's mungbean production. Due to its high nutritional content, mung bean is a major contributor to the Nation's economy. The present study was to isolate and characterize the new isolates of Cercospora canescens collected from coastal belts of Odisha, India. Methods: Surveys were conducted in the mung bean growing fields of Bhubaneswar (20.26N, 85.8E) and Berhampur (19.36N, 84.77E), coastal regions of Odisha during 2018-20. New Cercospora canescens isolates were collected and characterised through morphological, biochemical and molecular basis. Result: New Cercospora isolates were characterized on the basis of morphological, biochemical and molecular analysis to understand the gravity of the disease. Pathogenicity tests were conducted by artificial inoculation to identify the resistant and susceptible mung bean genotypes. On the basis of molecular analysis (internal transcribed spacer (ITS) region), there was a difference between the two isolates and forming different clades. These isolates were sequenced having the NCBI Acc. No. MZ475049 and MZ475050. Both isolates are different from each other because of transitional substitution of thymine and cytosine. The findings will be a complementary contribution for conservation and better management strategies of CLS disease in mung bean.
Legume Research- An International Journal
Assessment of Genetic Diversity for Cercospora Leaf Spot (CLS) Resistance in Mung Bean [Vigna radiata (L.) Wilczek] using SSR Markers2024 •
Background: CLS causes severe yield loss in mung bean. To sustain mung bean health, it is vital to include alleles that may be useful in resisting CLS. Therefore, in the present study, 90 mung bean genotypes were included for assessing genetic diversity using 66 SSR markers for CLS resistance. Methods: The mung bean crop was regularly monitored for the presence of pathogen and development of CLS disease in natural field conditions during pre rabi 2018 and pre rabi 2019. CLS screening assessments of germplasms were carried out using a 1-5 rating scale. Total genomic DNA was isolated from the leaf samples of all the genotypes and SSR genotyping was performed. The genotyping data were analysed by using GenAlEx 6.51b2 and TASSEL 5.0 software programme for genetic diversity parameters. Result: A moderate molecular diversity was observed in the panel population as a wide variation in alleles showed a range of 80 bp to 300 bp with the average PIC value of 0.40. The maximum percentage of polymorphic loci was 75.76% in the resistance genotypes followed by 56.06% in moderately resistant genotypes. The percentages of Shannon information among and within the population were found to be 44% and 56%, respectively. The archaeopterx tree differentiated the panel population into two major clusters, i.e., cluster I and cluster II, which were again sub divided into different sub-clusters and sub-sub clusters. These findings indicate that, the mung bean germplasm panel used in the present study could enrich the local gene pool and provide information for CLS resistance breeding.
European Journal of Plant Pathology
Cercospora zeina is the causal agent of grey leaf spot disease of maize in southern Africa2009 •
The aim of our study was to identify the causal agent of grey leaf spot disease of maize in southern Africa. Single-conidial cultures were recovered from maize leaves with typical disease symptoms sampled from several fields in South Africa, Zambia and Zimbabwe. Morphology, cultural characteristics, and a PCR-based test using Cercospora zeae-maydis and C. zeina-specific primer sets identified all single-conidial cultures as C. zeina. In addition, sequence alignment of DNA fragments of the internal transcribed spacer region (ITS1, ITS2, and the 5.8S gene) and elongation factor 1-α grouped all cultures in the same clade as the C. zeina ex-type culture CBS 118820. To by-pass cultivation of the slow-growing fungus, a rapid method to isolate DNA directly from lesions was successfully applied for PCR identification of C. zeina with species-specific ITS and histone primers. Koch’s postulates were fulfilled for C. zeina by artificially inoculating maize plants in a greenhouse, re-isolating conidia emerging from lesions and verifying pathogen identity with molecular techniques. These results provide evidence that confirms the presence of C. zeina and absence of C. zeae-maydis in commercial maize plantations in southern Africa.
Scientific Reports
Bacterial endophytes as indicators of susceptibility to Cercospora Leaf Spot (CLS) disease in Beta vulgaris LThe fungus Cercospora beticola causes Cercospora Leaf Spot (CLS) of sugar beet (Beta vulgaris L.). Despite the global importance of this disease, durable resistance to CLS has still not been obtained. Therefore, the breeding of tolerant hybrids is a major goal for the sugar beet sector. Although recent studies have suggested that the leaf microbiome composition can offer useful predictors to assist plant breeders, this is an untapped resource in sugar beet breeding efforts. Using Ion GeneStudio S5 technology to sequence amplicons from seven 16S rRNA hypervariable regions, the most recurring endophytes discriminating CLS-symptomatic and symptomless sea beets (Beta vulgaris L.ssp. maritima) were identified. This allowed the design of taxon-specific primer pairs to quantify the abundance of the most representative endophytic species in large naturally occurring populations of sea beet and subsequently in sugar beet breeding genotypes under either CLS symptomless or infection stages usi...
2011 •
MAKARA of Science Series
Multilocus Sequence Analysis of Cercospora spp. from Different Host Plant Families2013 •
2010 •
Legume Research- An International Journal
Management of Cercospora Leaf Spot of Mungbean [Vigna radiata (L.) Wilczek] using Fungicides and Host Resistance in Bundelkhand Region of Uttar Pradesh, India2024 •
Background: The pulses are important crop of Bundelkhand region of Uttar Pradesh. Among the pulses, mungbean is an important. Despite the multifaceted importance of this crop, its production could not be stabilized in the Uttar Pradesh as well as in the country due to several biotic constraints like diseases and insect-pests leading to considerable yield losses. Among several diseases affecting mungbean, cercospora leaf spot (CLS) caused by Cercospora canescens Ellis and Martin is one of the most important fungal diseases occurring regularly in the mungbean growing areas during the warm and humid weather. Methods: Experiments were conducted during two cropping seasons i.e. Kharif 2019 and 2020 to find out an efficient management strategy for this disease. In first experiment, 9 fungicides were assessed against CLS during both the seasons. In second experiment, 200 germplasm of mungbean were evaluated for resistance against cercospora leaf spot. Result: Among nine different fungicide treatments evaluated individually and as well as in combinations, spraying Carbendazim twice after first appearance of symptoms and second at 15 days' interval, gave maximum reduction in the incidence and severity of Cercospora leaf spot i.e. 45.57% and 58.45%, respectively along with significant enhancement (53.39%) in grain yield over the unprotected crop followed by treatment with Carbendazim + Mancozeb which resulted in 35.44% and 52.65% reduction in incidence and severity respectively and 44.88% enhancement in yield. Treatment with Captan + Hexaconazole was found to be least effective to reduce the disease incidence/ severity as well as to increase the crop yield. In second field experiment out of 200 mungbean genotypes evaluated against cercospora leaf spot during two consecutive crop seasons, four genotypes viz., PDM 04-123, PDM 54, EC520034-1 and EC 520022 were found to be resistant against cercospora leaf spot disease.
2016 •
Diseases of soybean caused byCercospora spp. are endemic throughout the world’s soy-bean production regions. Species diversity in the genusCercospora has been underesti-mated due to overdependence onmorphological characteristics, symptoms, and host associations. Currently, only two species (Cercospora kikuchii andC. sojina) are recognized to infect soybean;C. kikuchii causes Cercospora leaf blight (CLB) and purple seed stain (PSS), whereas C. sojina causes frogeye leaf spot. To assess cryptic speciation among path-ogens causing CLB and PSS, phylogenetic and phylogeographic analyses were performed with isolates from the top three soybean producing countries (USA, Brazil, and Argentina; col-lectively accounting for ~80 % of global production). Eight nuclear genes and one mitochon-drial gene were partially sequenced and analyzed. Additionally, amino acid substitutions conferring fungicide resistance were surveyed, and the production of cercosporin (a polyke-tide toxin produced bymanyCe...
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