Mycol. Res. 99 (2): 23G232 (1995) 230 Printed in Great Britain Three new species of Pseudocercospora from the Nepal Himalaya U S H A BUDATHOKI1 A N D S A N J A Y K. SINGHZ Central Department of Botany, Tribhuvan University, Kirtipur, Kathmandu, Nepal Department of Botany, University of Gorakhpur, U.P. India Pseudocercospora heterospermi sp. nov. on Heferospermurn pedunculosum Linn., P. himalayana sp. nov. on Triumfetta pilosa Roth. and P. smilacis sp. nov. on Smilax aspera Linn., collected from Kathmandu Valley in Nepal Himalaya, are described, illustrated and compared with allied taxa. Pseudocercospora heterospermi Budathoki & S. K. Singh sp. nov. (Fig. I) Maculae hypogenae, nervisequae, atrae, extendentes per 1/3 vel toturn folii; mycelium ex hyphis imrnersis, hyalinis, ramosis, laevibus; stromata substomatoidea, 16-26 x 13-26 pm. Conidiophora rnacronernatosa, mononematosa, caespitosa, recta vel flexuosa, plerumque ramosa, septata, simplicia, laevia, pallide olivacea vel bmnnea, Fig. 1. Pseudocercospora heterospermi, showing stroma (A), conidiophores (B), branched conidiophore (C) and conidia (D). Bar 20 prn. 32.5-65.5 x 2.54.5 pm. Cellulae conidiogenae in conidiophorsis incorporatae, denticulatae, polyblasticae. Conidia solitaria, simplicia, laevia, 3-7 septata, brunnea, 29-58.5 x 3 4 . 5 pm. In foliis vivis Heterospermi pedunculosi (Asteraceae), Godawari, Kathmandu Valley, Nepal, Nov. 1986, leg. Usha Budathoki, KU 60 holotypus, IMI 291865 isotypus. Colonies strictly hypogenous, vein limited, blackish patches coalescing and covering almost one third or more of the leaf surface. Mycelium composed of immersed, hyaline, branched, smooth-walled hyphae. Stromafa substomatal, bearing fasciculate conidiophores, more or less globose, pseudoparenchymatous, 16-26 x 13-26 pm. Conidiophores macronematous, mononematous, caespitose, straight or flexuous, sometimes branched, septate, simple, smooth, pale-olivaceous brown, fasciculate, 32.5-65.5 x 2-5-6.5 ym. Conidiogenous cells integrated, slightly denticulate, terminal, polyblastic. Conida solitary, simple, smooth, transversely 3-7 septate, brown, measuring 29-58.5 x 3-6-5 ym. There are no previous records of a Pseudocercospora species described on the host genus. However, 20 species of Pseudocercospora have so far been described on the Asteraceae (Deighton, 1976, 1987; Yen & Lim, 1980, 1983; Shukla ef al., 1982; Khan & Shamsi, 1983; Goh and Hsieh, 1989; Sarbajana & Das, 1990; Braun & Castaiieda, 1991). Amongst these 20 species, the well developed stromata and branching in the conidiophores of the present species are shared only by Pseudocercospora chysanfhmicola (J. M . Yen) Deighton and P. cinereae (Pavgi & U . P. Singh) Deighton. The present species is, therefore, compared with these and differences are shown in Table 1. It is clear that P. heterospermi is closer to P. cinereae in having a similar number of septa in the conidia but their dimensions are significantly different. As regards the length of conidiosphores, it is closer to P. chysanthemicola but differs in the length and septation of the conidia. Branched conidiophores U. Budathoki and S. K. Singh 23 1 Table I. Pseudocercospora species producing stromata and branched conidiophores described from Asteraceae (m) Shoma (vm) Conidiophores P. chysanthemicola Amphigenous, up to 35 diam. 0-4 septate, 2-20 in fascicles, 25-50 X 3-5 3-13 septate, 30-115 x 3-5 P. cinereae Hypogenous, 15-23 diam. Septate, 3-8 septate, 41-108.5 x 4.5-7 Hypogenous, Septate, 1626x13-26 32-5-65.5 x 2.54.5 P. heterospemi 16G400 x 4-6.5 Conidia (pm) 3-7 septate, 29-58.5 x 3 4 . 5 Fig. 2 . Psetldocercospora himalayana, showing stroma (A), conidiophores (B) and conidia (C). Bar 20 pm. are also produced by P. noveboracensis Goh & W . H. Hsieh and P. blUmeae(Thiim.) Deighton but these species do not produce stromata. Pseudocercospora himala~anaBudathoki & S. K. Singh sp. nov. (Fig. 2 ) Maculae hypogenae, griseae vel nigrae, 1-10 mrn diam., coalescentes per totum folii. Mycelium ex hyphis irnrnersis, hyalinis, rarnosis, Fig. 3. Pseudocercospora smilacis showing stroma (A), conidiophores (B), conidia (C) and germinating conidium (D). Bar 20 vm. laevibus. Stromata substomatales, usque 32.5-65.5 x 13-32.5 wn. Conidiophora rnacronernatosa, rnononernatosa, caespitosa, recta vel flexuosa, non-ramosa, septata, simplicia, laevia, pallide brunnea, 48.5-81.5 x 3-65 w. Cellulae conidiogenae in conidiophorsis incorporatae, denticulatae, terminales, polyblasticae. Conidia simplicia, Table 2. Pseudocercospora species described from Tiliaceae P. corchorica P. triumfettae P. hiumfettigena P. phaea P. himalayana Epigenous/l30 x 100 Amphigenous/absent Hypogenous/absent to poorly developed, 25 x 15 Amphigenous/absent Hypogenous/ 32.5-65.5 X 13-32.5 Conidia Conidiophores (vm) (m) Up to 80 x 3-5.5 %80 x 3-4 Up to 600 X 3-8 40-80 x 4-5, 1-7 septate 35-110 x 3-4, 3-10 septate 3@90 x 4-6, 2-10 septate 5G500 x 4.5-7 48.5-81.5 x 3+5 40-85 x 4-7, 1-7 septate, 101-1495 X 3 , 5 4 5 , 3-10 septate Three new species of Pseudocercospora 232 60-76 x 3-35 pm. Conidiogenous cells integrated, polyblastic, terminal or intercalary, denticulate, conidia solitary, simple, pale brown, smooth, 1-43 septate, obclavate, straight or curved, 48.5-81.5 3 4 . 5 A review of the literature reveals only one species of Cercospora described on Smilax (Chupp, 1954) which was Colonies hypogenous, greyish black, 1-10 mm diam., later subsequently transferred to Pseudocercospora as P. pallidissima coalescing and covering almost all the lower leaf surface. (Chupp) Deighton (Deighton, 1976). The present collection Mycelium composed of immersed, hyaline, branched, smooth was compared with P. pallidissima in which infection spots walled hyphae. Sfromata substomatal, with fasciculate conidioare suborbicular to irregular measuring 5 rnm diameter with phores, 32.5-65-5 x 13-32.5 pm. Conidiophores macronemafruiting on both leaf surfaces. Conidiophores in this species tous, mononematous, caespitose, straight or flexuous, unwere up to 30 x 5 LUI- and conidia are slightly smaller at branched, septate, simple, smooth, pale brown, 48.5-81-5 30-70 x 2-4.5 pm. In the present collection, however, the x 3-6-5 pm. Conidiogenous cells integrated, denticulate, infection spots are very distinct. These are restricted and terminal, polyblastic. Conidia simple, smooth, transversely represented by small (1-6 mm diam.) circular necrotic lesions. 3-10 septate, brown, cylindrical, 101-149.5 x 3.5-6.5 pm. The fruiting is entirely hypogenous. The conidia and Four species of Pseudocercospora, P. corchorica (Petrak & Cif.) conidiophores are also slightly larger. Deighton, P. friumfeffae (Syd.) Deighton, P. friumfeffigena Authors are thankful to Dr B. C. Sutton, International (J. M. Yen & Gilles) Deighton and P. phaea (Syd.) Deighton Mycological Institute, England for accession numbers and (Deighton, 1976) have already been described on hosts in the confirming the identity of the species and to Dr Karnal, Tiliaceae. Differences are shown in Table 2. Department of Botany, University of Gorakhur (U.P.), India It is clear that the present species is closest to P. phaea in for helpful suggestions. The senior author is also thankful to conidial dimensions and to P. corchorica in conidiophore the authorities of Tribhuvan University, Kathmandu (Nepal) characters but is not considered to be conspecific with any of for grant-in-aid under Faculty Development Programme. the previously described species of Pseudocercospora on Tiliaceae. REFERENCES acropleurogenosa, laevia, 3-10 septata, brunnea, cylindrica, vel obclavata, usque 101-149.5 x 3.5-6.5 urn. In foliis vivis Triumfettae pilosae (Tiliaceae). Boshan, Kathmandu Valley, Nepal, Dec. 1986, leg. Usha Budathoki, KU 204 holotypus, IMI 304425 isotypus. 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