ISSN (print) 0093-4666 © 2015. Mycotaxon, Ltd. ISSN (online) 2154-8889 MYCOTAXON http://dx.doi.org/10.5248/130.569 Volume 130, pp. 569–575 April–June 2015 Some new records of Uredinales from Khyber Pakhtunkhwa, Pakistan M. Fiaz1, H. Ahmad2, N.S. Afshan3*, & A.N. Khalid4 1 Department of Botany & 2Department of Genetics, Hazara University, Mansehra, Pakistan *Centre for Undergraduate Studies & 4Department of Botany, University of the Punjab, Quaid-e-Azam Campus, Lahore, 54590, Pakistan *Correspondence to: pakrust@gmail.com 3 Abstract — Uromyces ferulae and the telial stage of Cerotelium ici were collected from Khyber Pakhtunkhwa and are new records for Pakistan. Puccinia exhausta on Clematis grata and P. ustalis on Ranunculus hirtellus are reported as new host records for these rusts in Pakistan. Key words —Oghi forest, Pucciniales, rust fungi Introduction During the exploration of Uredinales of Khyber Pakhtunkhwa, Pakistan, rust-infected plants were collected from diferent localities and examined macro-microscopically. Among the specimens, Heracleum cachemiricum was found infected with Uromyces ferulae, which is a new record for Pakistan. he telial stage of Cerotelium ici on Ficus carica and F. palmata is an addition to the already reported stages of this rust from Pakistan. Puccinia exhausta on Clematis grata and P. ustalis on Ranunculus hirtellus are new host records for these rusts in Pakistan. his work brings the total of rust taxa recorded from Khyber Pakhtunkhwa to 174. Previously about 173 species of rust fungi have been reported from this area (Afshan & Khalid 2008, 2009; Afshan et al. 2008a,b,c,d, 2010; Ishaq et al. 2013). Materials & methods Freehand sections & scrape mounts of infected plant materials were made in lactic acid. he plants were photographed and infected portions were observed under a stereomicroscope. Twenty spores representing each spore state were examined under a Nikon YS 100 microscope, and paraphyses and spore were measured using a Zeiss 570 ... Fiaz & al. eyepiece screw micrometer. Sections, paraphyses and spores were microphotographed by Digiporo-Labomed. Spores and paraphyses were drawn with the aid of a camera lucida (Ernst Leitz Wetzlar Germany). Specimens are conserved in the Herbarium, Hazara University, Dhodial, Pakistan (HUP). New records Uromyces ferulae Juel, Bull. Soc. mycol. France 17: 259. 1901. Plate 1 Spermogonia and aecia were not found. Uredinia and telia are mixed in the same sori, amphigenous and petiicolous, rounded, in dense clusters, especially on the lealets, dark brown 0.09–0.26 × 0.25–0.41 mm. Urediniospores globoid or subgloboid, 22.4–24.1 × 26.9–27.9 µm (mean 23.4–27.6 µm), hyaline to golden brown, echinulate; germ pores 2–4, equatorial; pedicel hyaline, deciduous. Teliospores globose to subglobose or ovoid to ellipsoid, 17.7–23.7 × 29.3–37.8 µm (mean 22.2–33.4 µm), apex rounded; wall 1.4–2.6 µm, light brown to chestnut brown, smooth; apex 2.8–5.2 µm thick, germ pore mostly at the apex, pedicel hyaline, deciduous. Plate 1: Uromyces ferulae (HUP MFR-281): A. Teliospores. B. Urediniospores. Scale bars = 10 µm. Rusts and hosts new for Pakistan ... 571 Material examined: PAKISTAN, Khyber Pakhtunkhwa, District Mansehra, Oghi, Khabbal Paien, at 2046 m a.s.l., on Heracleum cachemiricum C.B. Clarke (Umbelliferae), stages II + III, May 2010, M. Fiaz #FR-208 (HUP MFR-281). Comments: Uromyces ferulae has been reported on diferent Ferula species from North Africa, southern Europe, Australia, and Asia (Iran) (Saccardo & Saccardo 1905: 248; Mercé 1975; Denchev 1995). In Pakistan, no species of Uromyces has previously been recorded on Umbelliferae. his is the irst report from Pakistan, and Heracleum cachemiricum represents a new host for Uromyces ferulae. Cerotelium ici (Castagne) Arthur, Bull. Torrey Bot. Club 44: 509. 1917. Plate 2 Spermogonia and aecia are unknown. Uredinia hypophyllous, scattered over the whole surface, minute, sometimes in large irregular groups, slightly Plate 2: Cerotelium ici (HUF MFR-252): C. Subepidermal telium. D. Incurved paraphyses. E. Urediniospores. Scale bars: C = 9 cm; D = 8.5 µm; E = 6 µm. 572 ... Fiaz & al. erumpent, pulverulent, reddish brown, surrounded by peripheral, incurved and basally united paraphyses. Urediniospores subglobose or broadly ellipsoid to obovoid or ovate, densely echinulate, yellowish brown, 16–21.7 × 17–31.2 µm, wall 1–1.6 µm thick, germ pores scattered, obscure. Telia hypophyllous, subepidermal, scattered, minute, 190–270 µm in diam. Teliospores one-celled, cylindrical, broadly ellipsoid or oblong, smooth, 8.7–11.4 × 14.6–20.8 µm, catenulate, in two or three superimposed layers, walls uniformly thin, colorless. Material examined: PAKISTAN, Khyber Pakhtunkhwa, District Mansehra, Bherkund, at 849 m a.s.l., on Ficus carica L. (Moraceae), stages II + III, Nov. 2010, M. Fiaz #FR-093 (HUP MFR-252); District Mansehra, Gulebagh, at 920 m a.s.l., on Ficus palmata Forssk., stage II, Oct. 2009, M. Fiaz #FR-089 (HUP MFR-253). Comments: he telial stage of Cerotelium ici is a new record for Pakistan. he uredinial stage of Cerotelium ici has been reported on Ficus carica, F. palmata, and F. religiosa L. from Lahore, Change Manga, Sangla Hill, Tandojam, Malir (Karachi), and Rawalpindi (Ahmad 1956a,b, Hasnain et al. 1959, Khan & Kamal 1968, Ghafar & Kai 1968, Kaneko 1993). Puccinia exhausta Dietel, Bot. Jb. 28: 283. 1900. Plate 3 Spermogonia are usually epiphyllous, subcutaneous, chestnut-brown, spherical or ellipsoid, 74–117 × 79–126 µm. Aecia and uredinia not found. Telia amphigenous, small, rounded, scattered or in circular groups, dark brown to blackish, erumpent or covered by epidermis. Teliospores mostly ellipsoid, sometimes 1-celled and elongated, conical or rounded at the apex, base mostly roundish, apical wall may be thickened up to 3 µm, sometimes 2-celled, not thickened, slightly constricted or non-constricted at the septum, smooth, hyaline to yellowish and chestnut brown, 17–24 × 26–47 µm (mean = 21 × 39 µm), wall 1.4–2.5 µm, germ pore apical in upper cell, adjacent to the pedicel or basal in lower cell, distinct colorless papilla over the germ pore, sometimes up to 4 µm; pedicels hyaline, deciduous, rarely up to 80 µm long. Material examined: PAKISTAN, Khyber Pakhtunkhwa, District Mansehra, Dhodial, at 978 m a.s.l., on Clematis grata Wall. (Ranunculaceae), stages 0 + III, Aug. 2010, M. Fiaz #FR-003. (HUP MFR-266). Comments: Puccinia exhausta is cosmopolitan and reported on diferent Clematis species (Hiratsuka & Chen 1991, Hiratsuka et al. 1992, Kobayashi 2007). From Pakistan, it has previously been reported on Clematis montana Buch.-Ham. ex DC. from the Kawai and Malakandi forests (Kaghan valley) by Ono & Kakishima (1992) and Ono (1992). Clematis grata represents a new host record for P. exhausta in Pakistan. Rusts and hosts new for Pakistan ... 573 Plate 3: Puccinia exhausta (HUP MFR-266): Teliospores. Scale bar = 10 µm. Puccinia ustalis Berk. Hooker’s J. Bot. Kew Gard. Misc. 6: 207. 1854. Plate 4 Spermogonia, aecia and uredinia are unknown. Telia usually hypophyllous as dark brown to blackish spots, scattered or aggregated in large groups, compact. Teliospores mostly 2-celled, occasionally 1- or 3-celled teliospores co-exist, ellipsoid, oblong to cylindrical or irregular, conical or rounded above and narrowed below, constricted at the septum, yellowish brown, pale brown basally; 2-celled spores 10–16 × 33–63 µm, smooth, 1–1.5 µm thick at side, apically 2–8.6 µm thick; germ pore one per cell, apical or sub apical in distal cell, in basal cell near the septum or obscure; 1-celled spores 8–11 × 17–33 µm, at side 1–1.5 µm, apices 2–5.4 µm thick; 3-celled spores 10–13 × 48–52 µm, 1–1.5 µm at sides and 2–6 µm at the apices. Pedicel pale yellow to hyaline, deciduous, 7–10 µm long. 574 ... Fiaz & al. Plate 4: Puccinia ustalis (HUF MFR-272): Teliospores. Scale bar = 12 µm. Material examined: PAKISTAN, Khyber Pakhtunkhwa, District Mansehra, Balakot, Nadibanglaw, at 2515 m a.s.l., on Ranunculus hirtellus Royle (Ranunculaceae), stage III, Jul. 2011, M. Fiaz #FR-005. (HUP MFR-272). Comments: Puccinia ustalis is cosmopolitan and reported on diferent species of Anemone, Clematis, Aquilegia, Pulsatilla, Ranunculus, halictrum, and Trautvetteria (Hiratsuka et al. 1992). From Pakistan, it has been reported on Anemone obtusiloba D. Don from Changla Gali and Nathia Gali (Ahmad 1956a,b) and on Ranunculus difusus DC. from Dunga Gali (Okane et al. 1992) and the Kaghan Valley (Ono 1992). Ranunculus hirtellus represents a new host record for Puccinia ustalis in Pakistan. Acknowledgements We sincerely thank Dr. Abdul Rehman Niazi (Department of Botany, University of the Punjab, Lahore, Pakistan) and Dr. Omar Paíno Perdomo (Dominican Society of Mycology, Santo Domingo, Dominican Republic) for their valuable suggestions to improve the manuscript and acting as presubmission reviewers. Literature cited Afshan NS, Khalid AN. 2008. New rust fungi on noxious weeds from Pakistan. Pak. J. Phytopathol. 20(1): 82–87. Afshan NS, Khalid AN. 2009. 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