Biodiversity Data Journal 3: e6313 doi: 10.3897/BDJ.3.e6313 Taxonomic Paper Biodiversity inventories in high gear: DNA barcoding facilitates a rapid biotic survey of a temperate nature reserve Angela C Telfer‡, Monica R Young‡, Jenna Quinn§, Kate Perez‡, Crystal N Sobel‡, Jayme E Sones‡, Valerie Levesque-Beaudin‡, Rachael Derbyshire‡, Jose Fernandez-Triana|, Rodolphe Rougerie¶, Abinah Thevanayagam‡, Adrian Boskovic‡, Alex V Borisenko‡, Alex Cadel#, Allison Brown‡, Anais Pages¤, Anibal H Castillo‡, Annegret Nicolai«, Barb Mockford Glenn Mockford», Belén Bukowski˄, Bill Wilson», Brock Trojahn§, Carole Ann Lacroix˅, Chris Brimblecombe¦, Christoper Hayˀ, Christmas Ho‡, Claudia Steinke‡, Connor P Warne‡, Cristina Garrido Cortesˁ, Daniel Engelking‡, Danielle Wright‡, Dario A Lijtmaer˄, David Gascoigne», David Hernandez Martich₵, Derek Morningstarℓ, Dirk Neumann₰, Dirk Steinke‡, Donna DeBruin Marco DeBruin», Dylan Dobiasˁ, Elizabeth Sears‡, Ellen Richardˁ, Emily Damstra», Evgeny V Zakharov‡, Frederic Labergeˁ, Gemma E Collins¦, Gergin A Blagoev‡, Gerrie Grainge», Graham Ansell‡, Greg Meredith₱, Ian Hogg¦, Jaclyn McKeown‡, Janet Topan‡, Jason Bracey», Jerry Guenther», Jesse Sills-Gilligan‡, Joseph Addesi‡, Joshua Persi‡, Kara K S Layton₳, Kareina D'Souza‡, Kencho Dorji₴, Kevin Grundy», Kirsti Nghidinwa₣, Kylee Ronnenberg‡, Kyung Min Lee₮, Linxi Xie₦, Liuqiong Lu‡, Lyubomir Penev₭, Mailyn Gonzalez₲, Margaret E Rosati‽, Mari Kekkonen‡, Maria Kuzmina‡, Marianne Iskandar‡, Marko Mutanen₮, Maryam Fatahi‡, Mikko Pentinsaari₮, Miriam Bauman», Nadya Nikolova‡, Natalia V Ivanova‡, Nathaniel Jones‡, Nimalka Weerasuriya₦, Norman Monkhouse‡, Pablo D Lavinia˄, Paul Jannetta‡, Priscila E Hanisch˄, R. Troy McMullin˅, Rafael Ojeda Flores₩, Raphaëlle Mouttet₸, Reid Vender‡, Renee N Labbee‡, Robert Forsyth‡‡, Rob Lauder§§, Ross Dickson», Ruth Kroft», Scott E Miller‽, Shannon MacDonald‡, Sishir Panthi||, Stephanie Pedersen‡, Stephanie Sobek-Swant§, Suresh Naik‡, Tatsiana Lipinskaya¶¶, Thanushi Eagalle‡, Thibaud Decaëns##, Thibault Kosuth¤, Thomas Braukmann‡, Tom Woodcock§, Tomas Roslin¤¤,««, Tony Zammit»», Victoria Campbell‡, Vlad Dinca‡, Vlada Peneva˄˄, Paul D N Hebert ‡, Jeremy R deWaard‡ ‡ Biodiversity Institute of Ontario, Guelph, Canada § rare Charitable Research Reserve, Cambridge, Canada | CNC, Ottawa, Canada ¶ Muséum national d'Histoire Naturelle, Paris, France # University of Waterloo, Waterloo, Canada ¤ Université de Montpellier, Montpellier, France « EcoBio, Université of Rennes, Rennes, France » rare Charitable Research Reserve (Affiliate of), Cambridge, Canada ˄ Museo Argentino de Ciencias Naturales "Bernardino Rivadavia" (MACN-CONICET), Buenos Aires, Argentina ˅ Biodiversity Institute of Ontario Herbarium, Guelph, Canada ¦ University of Waikato, Hamilton, New Zealand ˀ University of Western Ontario, London, Canada ˁ University of Guelph, Guelph, Canada © Telfer A et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Telfer A et al. 2 ₵ Universidad Autonoma de Santo Domingo DR, Santo Domingo, Dominican Republic ℓ Myotistar, Cambridge, Canada ₰ SNSB, Zoologische Staatssammlung Muenchen, Munich, Germany ₱ Grand River Conservation Authority, Guelph, Canada ₳ The University of Western Australia, Perth, Australia ₴ National Biodiversity Centre, Thimphu, Bhutan ₣ Ministry of Environment and Tourism in Namibia, Windhoek, Namibia ₮ University of Oulu, Oulu, Finland ₦ The University of Western Ontario, London, Canada ₭ Pensoft, Sofia, Bulgaria ₲ Instituto de Investigación de Recursos Biológicos Alexander von Humboldt, Bogotá, Colombia ‽ Smithsonian National Museum of Natural History, Washington, United States of America ₩ Universidad Nacional Autónoma de México, Mexico City, Mexico ₸ ANSES, Laboratoire de la Santé des Végétaux, Montferrier sur Lez, France ‡‡ New Brunswick Museum, Saint John, Canada §§ London Homeopathy, London, Canada || Ministry of Forests and Soil Conservation, Kathmandu, Nepal ¶¶ Scientific and Practical Center for Bioresources, National Academy of Sciences of Belarus, Minsk, Belarus ## Université de Montpellier Centre d'Ecologie Fonctionnelle et Evolutive, Montpellier, France ¤¤ University of Helsinki, Helsinki, Finland «« Swedish University of Agricultural Sciences, Uppsala, Sweden »» Grand River Conservation Authority, Cambridge, Canada ˄˄ Bulgarian Academy of Sciences, Sofia, Bulgaria Corresponding author: Angela C Telfer (atelfer@uoguelph.ca), Jeremy R deWaard (dewaardj@uoguelph.ca) Academic editor: Pavel Stoev Received: 24 Aug 2015 | Accepted: 24 Aug 2015 | Published: 30 Aug 2015 Citation: Telfer A, Young M, Quinn J, Perez K, Sobel C, Sones J, Levesque-Beaudin V, Derbyshire R, Fernandez-Triana J, Rougerie R, Thevanayagam A, Boskovic A, Borisenko A, Cadel A, Brown A, Pages A, Castillo A, Nicolai A, Glenn Mockford B, Bukowski B, Wilson B, Trojahn B, Lacroix C, Brimblecombe C, Hay C, Ho C, Steinke C, Warne C, Garrido Cortes C, Engelking D, Wright D, Lijtmaer D, Gascoigne D, Hernandez Martich D, Morningstar D, Neumann D, Steinke D, Marco DeBruin D, Dobias D, Sears E, Richard E, Damstra E, Zakharov E, Laberge F, Collins G, Blagoev G, Grainge G, Ansell G, Meredith G, Hogg I, McKeown J, Topan J, Bracey J, Guenther J, Sills-Gilligan J, Addesi J, Persi J, Layton K, D'Souza K, Dorji K, Grundy K, Nghidinwa K, Ronnenberg K, Lee K, Xie L, Lu L, Penev L, Gonzalez M, Rosati M, Kekkonen M, Kuzmina M, Iskandar M, Mutanen M, Fatahi M, Pentinsaari M, Bauman M, Nikolova N, Ivanova N, Jones N, Weerasuriya N, Monkhouse N, Lavinia P, Jannetta P, Hanisch P, McMullin R, Ojeda Flores R, Mouttet R, Vender R, Labbee R, Forsyth R, Lauder R, Dickson R, Kroft R, Miller S, MacDonald S, Panthi S, Pedersen S, Sobek-Swant S, Naik S, Lipinskaya T, Eagalle T, Decaëns T, Kosuth T, Braukmann T, Woodcock T, Roslin T, Zammit T, Campbell V, Dinca V, Peneva V, Hebert P, deWaard J (2015) Biodiversity inventories in high gear: DNA barcoding facilitates a rapid biotic survey of a temperate nature reserve. Biodiversity Data Journal 3: e6313. doi: 10.3897/BDJ.3.e6313 Abstract Background Comprehensive biotic surveys, or ‘all taxon biodiversity inventories’ (ATBI), have traditionally been limited in scale or scope due to the complications surrounding specimen sorting and species identification. To circumvent these issues, several ATBI projects have successfully integrated DNA barcoding into their identification procedures and witnessed Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... 3 acceleration in their surveys and subsequent increase in project scope and scale. The Biodiversity Institute of Ontario partnered with the rare Charitable Research Reserve and delegates of the 6th International Barcode of Life Conference to complete its own rapid, barcode-assisted ATBI of an established land trust in Cambridge, Ontario, Canada. New information The existing species inventory for the rare Charitable Research Reserve was rapidly expanded by integrating a DNA barcoding workflow with two surveying strategies – a comprehensive sampling scheme over four months, followed by a one-day bioblitz involving international taxonomic experts. The two surveys resulted in 25,287 and 3,502 specimens barcoded, respectively, as well as 127 human observations. This barcoded material, all vouchered at the Biodiversity Institute of Ontario collection, covers 14 phyla, 29 classes, 117 orders, and 531 families of animals, plants, fungi, and lichens. Overall, the ATBI documented 1,102 new species records for the nature reserve, expanding the existing long-term inventory by 49%. In addition, 2,793 distinct Barcode Index Numbers (BINs) were assigned to genus or higher level taxonomy, and represent additional species that will be added once their taxonomy is resolved. For the 3,502 specimens, the collection, sequence analysis, taxonomic assignment, data release and manuscript submission by 100+ co-authors all occurred in less than one week. This demonstrates the speed at which barcode-assisted inventories can be completed and the utility that barcoding provides in minimizing and guiding valuable taxonomic specialist time. The final product is more than a comprehensive biotic inventory – it is also a rich dataset of finescale occurrence and sequence data, all archived and cross-linked in the major biodiversity data repositories. This model of rapid generation and dissemination of essential biodiversity data could be followed to conduct regional assessments of biodiversity status and change, and potentially be employed for evaluating progress towards the Aichi Targets of the Strategic Plan for Biodiversity 2011–2020. Keywords DNA barcoding, species identification, biodiversity assessment, biotic inventory, Barcode Index Numbers, Operational Taxonomic Units, rare Charitable Research Reserve Introduction It is now universally accepted that we have entered a period of unprecedented global biodiversity loss (Simberloff 1996, Pimm et al. 1995, Pimm et al. 2014), and quantifying this diversity rapidly and on a massive scale is required to begin the challenging process of halting this trend. The completion of biodiversity inventories at various geographic and time scales can contribute to national and international assessments of biodiversity knowledge, deemed necessary by the newly established Intergovernmental Science-Policy Platform on 4 Telfer A et al. Biodiversity and Ecosystem Services (Díaz et al. 2015). These assessments are fundamental for evaluating progress towards – and potentially reaching – the Convention on Biological Diversity’s Aichi Targets of the Strategic Plan for Biodiversity 2011–2020 (http s://www.cbd.int/sp/targets/). Specifically, biodiversity inventories address a component of Aichi target 19, to improve and disseminate biodiversity knowledge, particularly its status and trends. Even prior to the concept’s introduction (Janzen and Hallwachs 1994), several ‘all taxon biodiversity inventories’ (ATBI) and similar initiatives emerged to document large blocks of life in a circumscribed region or protected area. These comprehensive biotic surveys, particularly those in tropical locales, have traditionally been mired at the stage of specimen sorting and species identification. The taxonomic impediment – the shortage of taxonomic information and the gaps in our taxonomic knowledge – have severely limited and slowed the sorting and naming of collected material (Janzen 1993, Lawton et al. 1998, Janzen 2004). The integration of DNA barcoding however, has simplified, accelerated, and democratized this task (Hebert et al. 2003, Packer et al. 2009, Cristescu 2014, Joly et al. 2014). Several ATBI projects have now successfully integrated DNA barcoding into their identification procedures, observed this acceleration, and in many cases, even increased the scope or scale of their project as a result. A few notable ATBIs that have incorporated DNA barcoding for species identification include projects in the Área de Conservación Guanacaste, Costa Rica (Janzen et al. 2009), Churchill, Canada (Zhou et al. 2009), Great Smoky Mountains, United States (Zhou et al. 2011), Madang, Papua New Guinea (Novotny et al. 2007), Moorea, French Polynesia (Check 2006), Zackenberg, Greenland (Wirta et al. 2015), and Mount Kinabalu, Malaysia (Merckx et al. 2015). Following this model, the present study introduces DNA barcoding to a long-term biotic inventorying effort being conducted in a temperate nature reserve. The objective is to gauge the effect of adding this tool, in terms of both acceleration and increase of taxonomic scope, while concurrently constructing a reference DNA barcode library to facilitate future research and monitoring at this site. The existing inventory is expanded by employing two surveying strategies – a longer and comprehensive invertebrate trapping scheme, followed by a concentrated effort involving taxonomic experts in the form of a bioblitz (Lundmark 2003). In both cases, DNA barcoding is employed to sort the material rapidly into operational taxonomic units (OTUs), provide taxonomic assignment at varying levels of resolution depending on the taxon group, and organize the OTUs and linked specimen vouchers for examination by experts. The results demonstrate the speed at which barcode-assisted surveys can be completed, the role that barcoding plays in limiting and optimizing valuable taxonomic specialist time, and ultimately, a scalable model for rapid biotic surveys and dissemination of the rich biodiversity data captured. The product is not merely a comprehensive biotic inventory, but also a rich dataset of fine-scale occurrence and sequence data, all stored and cross-linked in several public biodiversity data repositories. Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... 5 Materials and methods Study Site and Existing Species Inventory The rare Charitable Research Reserve is a 365+ hectare land reserve which was set aside in 2001 to preserve the cultural history and ecological integrity of the area, while providing opportunities for scientific research and public education within the context of an urbanized region. It is located at the confluence of the Speed and Grand Rivers in Cambridge, Ontario, Canada (43.381128, -80.357807), where the Carolinian and Northern Hardwood forests also meet. The reserve contains a diversity of habitats including existing and reclaimed agricultural lands, wetlands, floodplains, shrub thickets, limestone cliffs and alvars, cold-water creeks, and old growth forest. Due to these diverse habitats, as well as the organization’s mandate to facilitate scientific research, rare has been the site of a variety of innovative research studies, including studies on fern genetics (Henry et al. 2014), prairie community establishment (Harvey and MacDougall 2014), and pollination services (Woodcock et al. 2014). For the present study, we chose sampling sites to encompass a wide range of habitats within the area. In advance of the bioblitz, sampling was conducted within an alvar, reclaimed agricultural fields, forest edges, and a wetland (Fig. 1). Six additional sites were sampled for the bioblitz: terrestrial and aquatic sampling was performed at the Grand River, Blair Flats Wetland, and Cruickston Creek; terrestrial sampling was conducted at an alvar, a cedar stand, and a silver maple wetland (Fig. 1). The wetlands on the reserve are part of the Barrie's Lake Bauman Creek Wetland Complex, which has been classified as a Provincially Significant Wetland by the Ontario Ministry of Natural Resources. Much of the property is also considered locally significant by the Regional Municipality of Waterloo. Figure 1. Map indicating habitat types and sampling sites for the 2015 biotic survey conducted at rare Charitable Research Reserve in Cambridge, Ontario, Canada. 6 Telfer A et al. Prior to this study, 2,246 species had been recorded at rare, including birds (231), mammals (37), insects (832), plants (836), mosses (63) and lichens (21) (Suppl. material 1; note that 218 species still require confirmation, denoted by parentheses). These observations have come from a variety of sources including rare staff and advisors, citizen scientists, and academic researchers, some of whom have made notable contributions to this inventory, such as Woodcock et al. 2014 who added 150 species of pollinators. Others have provided observations of rarely encountered species, such as the rove beetles Xantholinus elegans (Olivier, 1795) (first record for North America) and Xantholinus linearis (Olivier, 1794) (previously known from the east coast of Canada, now present in Ontario) (Brunke and Majka 2010). The rare reserve also hosts several species that fall on the provi ncial or national lists of conservation concern: 1% of the total have a status ranging from special concern, threatened or endangered (15, 6 and 7 species, respectively), while 1.5% have an undefined status of rare or uncommon (15 and 19 species, respectively). Several of these species, such as the barn swallow [Hirundo rustica (Linnaeus, 1758)], which has been nationally listed as threatened since 2011, are the subjects of active recovery projects at rare. Similar to many species inventories, rare's list is evidently more complete for charismatic and well-studied taxa, such as birds, butterflies and vascular plants. The observations for these groups would be enabled by excellent field guides and the disproportionate interest of citizen scientists; the opposite would be the case for most invertebrate taxa, and rare's inventory reflects this. If it is assumed that the proportions of major taxonomic groups inhabiting this reserve resemble those for similar temperate sites (e.g., Great Smoky Mountains: Sharkey 2001) or the Canadian terrestrial and freshwater biota in general (Mosquin et al. 1995), this inventory is deficient in several major groups such as insects, arachnids, nematodes, fungi and lichens. The supporting data for most species entries are also lacking, such as specific locality, date or identifier of each observation. In addition, the bioblitz has highlighted errors in the inventory which may have been present due to the compilation of observations from a variety of sources including non-experts. Furthermore, nearly all observations are not supported by voucher specimens or images to permit verification (Bortolus 2008). Survey Strategies and Specimen Collection Two strategies were employed in an effort to maximize the diversity of organisms inventoried. The first was a comprehensive collecting scheme executed over a period of approximately four months (May to August 2015). It involved a variety of targeted taxa and techniques, but heavily favoured the collection of terrestrial arthropods by passive trapping. Four Malaise traps were set up in various habitats around the rare property (Fig. 1) and were serviced weekly. Three pitfall traps were set up in close proximity to each Malaise trap and serviced in parallel. From May 25-31 and July 6-12, 2015, 'standardized sampling' procedures developed by the Biodiversity Institute of Ontario were employed at three different sites at rare (Fig. 1). Standardized sampling includes 20 pitfall traps, 10 pan traps, three litter and/or soil samples for Berlese funnels, one flight intercept trap and one Malaise trap – all deployed for a total of seven days. Standardized sampling also includes 60 total minutes of sweep netting, which is performed in three sessions with four collectors sweep netting for five consecutive minutes simultaneously. Each session is pooled into one Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... 7 sample and preserved in 95% ethanol. Passive traps (pitfall, pan, intercept) were deployed with soapy water and serviced every two days, while the Malaise trap used 95% ethanol and ran for the duration of the collecting period. All specimens collected through this standardized sampling routine were consolidated into individual jars for each technique. From July 23 to Aug 13, 2015, at least two ultraviolet light traps were deployed for one night per week; each trap was left overnight using ethyl acetate as a killing agent. On August 5, 2015, several aquatic samples were collected using stable dip nets for surber sampling, deploying hand nets along the stream banks and selective turning of stones to recover macroinvertebrates hiding below. Then the sample was passed through a 50 µm mesh net to capture the smaller invertebrates. All specimens collected from these samples were preserved in 95% ethanol. This first strategy of comprehensive sampling over several months was conducted by technical staff, including undergraduate students, and completed on Aug 15, 2015. The second strategy for surveying the reserve was a more concentrated effort and involved taxonomic experts – the execution of a bioblitz (Lundmark 2003). Termed the 'rare BioBlitz', the event involved 113 participants from 31 institutions and coincided with the 6th International Barcode of Life Conference (Adamowicz 2015). Collection efforts at the bioblitz were focused on six sites selected based on habitat diversity, proximity, and potential species diversity (Fig. 1). Sampling efforts were concentrated between 1-3 pm and 9-11 pm and targeted taxa that fell within the expertise of the participants and in groups underrepresented in the rare inventory (e.g., spiders, parasitic wasps, mites, nematodes, fungi, and lichens). Various methods for collecting both terrestrial and aquatic arthropods were employed, including dip nets, seine nets, sweep netting, and plankton netting. Small teams surveyed the property and adjacent rivers for taxonomic groups not collected in the four previous months, including fungi, lichens, and vascular plants. Since the inventory is fairly complete for vascular plants (836 species), an effort was made to barcode all species of Blair Flats, a tall grass prairie site that is an active research site (e.g., Harvey and MacDougall 2014). For these taxa, specimens were collected in the field into bags and processed afterward (e.g., pressed and dried onto herbarium sheets). Fungal specimens were collected and processed in a similar manner. Several taxa were targeted for sightings only, where no voucher specimens were collected; these groups included fish, birds, bats, herptiles and odonates (for bat sightings protocol, see Suppl. material 2). All collected specimens were sorted and identified to the lowest taxonomic level possible by the appropriate expert, both on site and following the event from August 16-20, 2015. Vertebrates, plants, fungi and lichens were almost all identified to species, while most invertebrate specimens were assigned to order or family prior to analysis. Invertebrate specimens were either stored in 95% ethanol or pinned after collection. For all invertebrate, plant, and fungal taxa, voucher specimens were collected where possible and deposited in the Biodiversity Institute of Ontario's natural history collection (BIOUG) or herbarium (BIO-OAC) for permanent storage (Schilthuizen et al. 2015). 8 Telfer A et al. DNA Barcode Analysis Both surveying strategies provided a large number of specimens that were sorted and prepared for subsequent DNA barcode analyses at the Canadian Centre for DNA barcoding (CCDB; www.ccdb.ca). A total of 25,287 specimens were sequenced from collection efforts from May to August 2015, followed by 3,502 specimens directly following the rare BioBlitz on Aug 16, 2015. Tissue samples were prepared in 96-well plate format and when necessary, the whole specimen proceeded through lysis and was recovered as voucher from the filter plate (Porco et al. 2010). Tissue lysis and DNA extraction varied slightly for different taxa (Suppl. material 3), but followed standard CCDB procedures (Ivanova et al. 2006, deWaard et al. 2008, Ivanova et al. 2008, Ivanova et al. 2011, Fazekas et al. 2012). One or more standard DNA barcode markers were targeted for each major group of organisms: for animals, the mitochondrial gene cytochrome oxidase subunit 1 (COI) (Hebert et al. 2003); for plants, the plastid marker rbcL (Hollingsworth et al. 2009) and the nuclear ribosomal internal transcribed spacer 2 (ITS2) marker (Hollingsworth 2011, Schoch et al. 2012); and for fungi and lichens (where only the fungal component was targeted), the internal transcribed spacer (ITS) marker (Schoch et al. 2012). PCR amplification, cycle sequencing and sequence analysis followed typical CCDB protocols (Ivanova and Grainger 2006, Kuzmina and Ivanova 2011). The primer cocktails used for PCR and sequencing are detailed in Suppl. material 3. The sequences were manually assembled and edited before upload to in the Barcode of Life Data Systems (BOLD, www.boldsystems.org) (Ratnasingham and Hebert 2007). The DNA extracts for all specimens are stored in the DNA Archive of the CCDB where they are available for additional study. Barcode Index Numbers and Taxonomic Assignment For the sequences derived from animal specimens, the records were assigned operational taxonomic units (OTUs) called Barcode Index Numbers (BINs) by the Refined Single Linkage (RESL) algorithm implemented on BOLD (Ratnasingham and Hebert 2013). For the sequences that have at least 500 bp coverage of the barcode region, < 1% ambiguous bases, and no stop codon or contamination flags, the RESL algorithm calculates the number of clusters and their membership (see Ratnasingham and Hebert 2013). The RESL algorithm runs weekly on all qualifying barcode sequences in BOLD, which as of August 2015, includes 5M specimens and 420K BINs. The BIN system is accessible through public, individual ‘BIN pages’ and permits rapid diversity assessments, even in the absence of taxonomic information. BINs show a high concordance with traditional taxonomic species names and can be used as a reliable proxy for species. For each specimen that was assigned an existing BIN, the record received the existing identification of the BIN to the lowest level that did not have taxonomic conflict. For each specimen assigned a new BIN for BOLD, the sequence was queried through the BOLD Identification Engine (BOLD-ID Engine; http://www.boldsystems.org/index.php/IDS_Open IdEngine). Identifications were applied based on sequence similarity (<15% for family, <5% Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... 9 for genus) if the query sequence fell within a monophyletic cluster of BINs assigned to this family or genus. For animal records that did not receive BINs (<500bp), the sequence was similarly queried through the BOLD-ID Engine, but used a <2% similarity cutoff for assignment to species, in addition to the genus and family thresholds. Following this, a neighbour-joining tree was constructed and examined for unexpected placements which might indicate overlooked contamination events or analytical error. Finally, specimens and images were inspected morphologically to check for errors and refine the assigned taxonomy where possible. Data resources Collection data, taxonomic assignment, sequence, electropherograms and primer details for each specimen record, and often a high resolution image, are available on BOLD in the public dataset, "rare BioBlitz 2015 [DS-RBB15]" (http://dx.doi.org/10.5883/DS-RBB15 or http://boldsystems.org/index.php/MAS_Management_OpenDataSet?datasetcode=DSRBB15). The sequence data for each successfully barcoded specimen were deposited to GenBank by using the 'Submit to GenBank' function in the BOLD workbench (see Suppl. material 4 for accession numbers). With the 'Data Spreadsheets' function in the BOLD workbench, the complete dataset was downloaded and reformatted into a Darwin Core Archive (Suppl. material 5) for upload to the Canadensys repository (http://www.canadensys.net), Canada's national node for the Global Biodiversity Information Facility (http://www.gbif.org). The online resource (http:// doi.org/10.5886/hh6td9jn) contains all records of the 2015 inventory, including human observations. The citation for the resource is as follows: Telfer A, Young MR, Quinn J, Perez K, Sobel CN, Sones JE, Levesque-Beaudin V, Derbyshire R, Fernandez-Triana J, Rougerie R, Hebert PDN, deWaard JR and contributors* (2015+). Inventory and BioBlitz Records from rare Charitable Research Reserve. 28,916 records. Online at http://data.canadensys.net/ipt/resource.do?r=rare_ inventory, http://doi.org/10.5886/hh6td9jn, and http://www.gbif.org/dataset/09e90dfb-5b1b4dd9-a796-e2fba53d26f0, released on 2015-08-20, version 1. GBIF key: 09e90dfb-5b1b-4dd9-a796-e2fba53d26f0. * See Suppl. material 6 for complete list of contributors, institutions and email addresses 10 Telfer A et al. Checklist of species observed or collected at the rare Charitable Research Reserve in Cambridge, Ontario, Canada. The first of five checklists for Kingdom Animalia, this checklist contains members of Phylum Annelida and Phylum Arthopoda (Class Arachnida and Class Insecta up to Order Dermaptera). Kingdom Animalia Phylum Annelida Class Clitellata Order Arhynchobdellida Family Erpobdellidae Erpobdella punctata Leidy, 1870 Order Haplotaxida Family Lumbricidae Lumbricus terrestris Linnaeus, 1758 Phylum Arthropoda Class Arachnida Order Araneae Family Agelenidae Agelenopsis potteri Blackwall, 1846 Family Amaurobiidae Callobius bennetti Blackwall, 1846 Notes: BOLD:AAB8212 Family Anyphaenidae Anyphaena celer Hentz, 1847 Anyphaena pectorosa L. Koch, 1866 Notes: BOLD:AAD6926 Hibana gracilis Hentz, 1847 Notes: BOLD:AAN6394 Wulfila saltabundus Hentz, 1847 Notes: BOLD:AAC6924 Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Family Araneidae Acanthepeira stellata Walckenaer, 1805 Notes: BOLD:AAD7855 Araneus diadematus Clerck, 1757 Notes: BOLD:AAA4125 Araneus trifolium Hentz, 1847 Notes: BOLD:AAB8544 Araniella displicata Hentz, 1847 Notes: BOLD:AAA8399 Argiope aurantia Lucas, 1833 Notes: BOLD:AAB7933 Argiope trifasciata Forsskål, 1775 Eustala anastera Walckenaer, 1841 Notes: BOLD:AAL4913 Eustala cepina Walckenaer, 1841 Notes: BOLD:AAB7935 Eustala emertoni Banks, 1904 Hypsosinga pygmaea Sundevall, 1831 Notes: BOLD:ABX6180 Hypsosinga rubens Hentz, 1847 Notes: BOLD:AAN6264 Larinioides cornutus Clerck, 1757 Notes: BOLD:AAA8999 Larinioides patagiatus Clerck, 1757 Mangora gibberosa Hentz, 1847 Notes: BOLD:AAB7330 Mangora maculata Keyserling, 1865 Mangora placida Hentz, 1847 Notes: BOLD:AAI4456|BOLD:ACE4103 Neoscona arabesca Walckenaer, 1841 Notes: BOLD:AAA4123 Family Clubionidae Clubiona abboti L. Koch, 1866 Notes: BOLD:AAD1564 11 12 Telfer A et al. Clubiona bryantae Gertsch, 1941 Clubiona johnsoni Gertsch, 1941 Notes: BOLD:AAN4847 Clubiona maritima L. Koch, 1867 Notes: BOLD:AAI4085 Clubiona obesa Hentz, 1847 Notes: BOLD:AAD5417 Clubiona pallidula Clerck, 1757 Notes: BOLD:AAI4087 Family Dictynidae Cicurina brevis Emerton, 1890 Notes: BOLD:AAC8284 Cicurina itasca Chamberlin & Ivie, 1940 Notes: BOLD:AAI4031 Cicurina pallida Keyserling, 1887 Notes: BOLD:AAF3046 Dictyna bellans Chamberlin, 1919 Notes: BOLD:AAI6249 Dictyna bostoniensis Emerton, 1888 Notes: BOLD:AAL1061 Dictyna brevitarsa Emerton, 1915 Notes: BOLD:AAB2306 Dictyna foliacea Hentz, 1850 Notes: BOLD:AAI6247 Dictyna volucripes Keyserling, 1881 Notes: BOLD:AAB1638|BOLD:ACE2869 Emblyna annulipes Blackwall, 1846 Emblyna hentzi Kaston, 1945 Notes: BOLD:AAI6251 Emblyna manitoba Ivie, 1947 Notes: BOLD:AAI9209 Emblyna sublata Hentz, 1850 Notes: BOLD:AAA7272 Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Family Gnaphosidae Drassyllus depressus Emerton, 1890 Notes: BOLD:AAD8676 Drassyllus niger Banks, 1896 Notes: BOLD:AAI9037 Gnaphosa parvula Banks, 1896 Notes: BOLD:AAC3779 Haplodrassus signifer C. L. Koch, 1839 Notes: BOLD:AAD0462 Herpyllus ecclesiasticus Hentz, 1832 Notes: BOLD:AAF2106 Micaria pulicaria Sundevall, 1831 Notes: BOLD:AAC6612 Sergiolus ocellatus Walckenaer, 1837 Notes: BOLD:ACV6055 Zelotes hentzi Barrows, 1945 Notes: BOLD:AAA8914 Zelotes pseustes Chamberlin, 1922 Family Hahniidae Neoantistea agilis Keyserling, 1887 Notes: BOLD:ACV5090 Neoantistea gosiuta Gertsch, 1934 Notes: BOLD:AAG9583 Family Linyphiidae Agyneta fabra Keyserling, 1886 Notes: BOLD:AAE3860 Agyneta micaria Emerton, 1882 Notes: BOLD:AAN6265 Agyneta unimaculata Banks, 1892 Notes: BOLD:AAH0003 Bathyphantes brevis Emerton, 1911 Notes: BOLD:AAC5851 13 14 Telfer A et al. Bathyphantes pallidus Banks, 1892 Notes: BOLD:AAC9112 Centromerus sylvaticus Blackwall, 1841 Notes: BOLD:AAA4132 Ceraticelus atriceps O. P.-Cambridge, 1874 Notes: BOLD:AAI3701 Ceraticelus similis Banks, 1892 Notes: BOLD:AAF1318 Ceratinella brunnea Emerton, 1882 Notes: BOLD:AAD2101 Ceratinops crenatus Emerton, 1882 Notes: BOLD:ACF8798 Ceratinops latus Emerton, 1882 Notes: BOLD:AAI5447 Ceratinopsis auriculata Emerton, 1909 Notes: BOLD:ACR6338 Ceratinopsis labradorensis Emerton, 1925 Notes: BOLD:ACV5182 Collinsia plumosa Emerton, 1882 Notes: BOLD:AAM9146 Eridantes erigonoides Emerton, 1882 Notes: BOLD:AAH0004 Erigone atra Blackwall, 1833 Notes: BOLD:ACE5877 Erigone autumnalis Emerton, 1882 Notes: BOLD:AAH0001 Erigone blaesa Crosby & Bishop, 1928 Notes: BOLD:ACE9601 Frontinella communis Hentz, 1850 Notes: BOLD:AAE0825 Grammonota angusta Dondale, 1959 Notes: BOLD:AAD1498 Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Grammonota inornata Emerton, 1882 Notes: BOLD:ACV5737 Hypomma marxi Keyserling, 1886 Notes: BOLD:AAB9520 Hypselistes florens O. P.-Cambridge, 1875 Notes: BOLD:AAB4233 Mermessus index Emerton, 1914 Notes: BOLD:ACL4554 Mermessus trilobatus Emerton, 1882 Notes: BOLD:AAC8898 Microlinyphia mandibulata Emerton, 1882 Notes: BOLD:AAF4994 Neriene clathrata Sundevall, 1830 Notes: BOLD:AAA8358|BOLD:AAB7327 Neriene montana Clerck, 1757 Neriene variabilis Banks, 1892 Pityohyphantes costatus Hentz, 1850 Pityohyphantes subarcticus Chamberlin & Ivie, 1943 Pocadicnemis americana Millidge, 1976 Notes: BOLD:AAC9060 Tennesseellum formica Emerton, 1882 Notes: BOLD:AAG5631 Tenuiphantes zebra Emerton, 1882 Notes: BOLD:AAI8098 Walckenaeria directa O. P.-Cambridge, 1874 Notes: BOLD:AAH8313 Walckenaeria fallax Millidge, 1983 Notes: BOLD:AAH8314 Walckenaeria pinocchio Kaston, 1945 Notes: BOLD:ACT1115 Walckenaeria spiralis Emerton, 1882 Notes: BOLD:AAH8314 15 16 Telfer A et al. Family Lycosidae Arctosa emertoni Gertsch, 1934 Notes: BOLD:ACW1682 Pardosa distincta Blackwall, 1846 Notes: BOLD:AAC7802 Pardosa milvina Hentz, 1844 Notes: BOLD:AAB7668 Pardosa modica Blackwall, 1846 Notes: BOLD:AAA5090 Pardosa moesta Banks, 1892 Notes: BOLD:AAB0863 Pardosa saxatilis Hentz, 1844 Notes: BOLD:AAB7667 Pirata piraticus Clerck, 1757 Notes: BOLD:AAB6784 Pirata praedo Kulczynski, 1885 Notes: BOLD:AAC5349 Piratula cantralli Wallace & Exline, 1978 Notes: BOLD:ABZ5613 Piratula minuta Emerton, 1885 Notes: BOLD:AAE4247 Schizocosa avida Walckenaer, 1837 Notes: BOLD:AAD3880 Schizocosa crassipalpata Roewer, 1951 Notes: BOLD:AAC4687 Schizocosa mccooki Montgomery, 1904 Notes: BOLD:AAH0055 Schizocosa ocreata Hentz, 1844 Notes: BOLD:AAA7232 Trochosa ruricola De Geer, 1778 Notes: BOLD:AAB0726 Trochosa terricola Thorell, 1856 Notes: BOLD:AAB0727 Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Family Mimetidae Mimetus epeiroides Emerton, 1882 Notes: BOLD:AAG5658 Mimetus haynesi Gertsch & Mulaik, 1940 Notes: BOLD:AAK6284 Mimetus notius Chamberlin, 1923 Family Philodromidae Philodromus cespitum Walckenaer, 1802 Notes: BOLD:AAB3836 Philodromus imbecillus Keyserling, 1880 Notes: BOLD:AAI2838 Philodromus praelustris Keyserling, 1880 Notes: BOLD:AAD2665 Philodromus rufus subsp. vibrans Dondale Thanatus coloradensis Keyserling, 1880 Notes: BOLD:AAM7986 Tibellus maritimus Menge, 1875 Notes: BOLD:AAA7188 Tibellus oblongus Walckenaer, 1802 Notes: BOLD:AAA7188 Family Phrurolithidae Phrurotimpus borealis Emerton, 1911 Notes: BOLD:AAC7234 Scotinella pugnata Emerton, 1890 Notes: BOLD:AAK7452 Family Pisauridae Dolomedes striatus Giebel, 1869 Notes: BOLD:ACI5773 Dolomedes tenebrosus Hentz, 1844 Pisaurina mira Walckenaer, 1837 Notes: BOLD:AAI2721 Family Salticidae Eris militaris Hentz, 1845 Notes: BOLD:AAA5654 17 18 Telfer A et al. Evarcha hoyi Peckham & Peckham, 1883 Notes: BOLD:AAC0342|BOLD:ACL8050 Habronattus decorus Blackwall, 1846 Marpissa formosa Banks, 1892 Notes: BOLD:AAG0312 Naphrys pulex Hentz, 1846 Notes: BOLD:AAC2433 Neon nelli Peckham & Peckham, 1888 Notes: BOLD:AAD9221 Pelegrina galathea Walckenaer, 1837 Notes: BOLD:AAB2930 Pelegrina insignis Banks, 1892 Notes: BOLD:AAB2928 Pelegrina proterva Walckenaer, 1837 Phidippus audax Hentz, 1845 Phidippus clarus Keyserling, 1885 Salticus scenicus Clerck, 1757 Sitticus floricola subsp. palustris Peckham and Peckham, 1883 Synageles noxiosus Hentz, 1850 Notes: BOLD:ACL8115 Tutelina harti Peckham, 1891 Notes: BOLD:AAW8769 Tutelina similis Banks, 1895 Notes: BOLD:AAF6387 Zygoballus nervosus Peckham & Peckham, 1888 Notes: BOLD:ACA1490 Family Tetragnathidae Leucauge venusta Walckenaer, 1841 Notes: BOLD:AAB8714 Pachygnatha dorothea McCook, 1894 Notes: BOLD:AAE5304 Pachygnatha tristriata C. L. Koch, 1845 Notes: BOLD:AAF1571 Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Pachygnatha xanthostoma C. L. Koch, 1845 Notes: BOLD:ACO7247|BOLD:ACP5884|BOLD:ACU5364 Tetragnatha caudata Emerton, 1884 Notes: BOLD:AAE3958|BOLD:ACN4034|BOLD:AAP3715 Tetragnatha extensa Linnaeus, 1758 Tetragnatha guatemalensis O. P.-Cambridge, 1889 Tetragnatha laboriosa Hentz, 1850 Notes: BOLD:AAA6381|BOLD:ACR6860 Tetragnatha shoshone Levi, 1981 Notes: BOLD:AAB7995 Tetragnatha straminea Emerton, 1884 Notes: BOLD:AAD7095 Tetragnatha viridis Walckenaer, 1841 Notes: BOLD:AAG5659|BOLD:AAN6335 Family Theridiidae Dipoena nigra Emerton, 1882 Notes: BOLD:AAF4974 Enoplognatha caricis Fickert, 1876 Notes: BOLD:AAO3896 Enoplognatha ovata Clerck, 1757 Notes: BOLD:AAA6910 Euryopis funebris Hentz, 1850 Notes: BOLD:AAJ0542 Hentziectypus globosus Hentz, 1850 Notes: BOLD:AAN6263 Neospintharus trigonum Hentz, 1850 Notes: BOLD:AAB0273 Neottiura bimaculata Linnaeus, 1767 Notes: BOLD:AAK8332|BOLD:ACN7831 Parasteatoda tabulata Levi, 1980 Parasteatoda tepidariorum C. L. Koch, 1841 Notes: BOLD:AAC0175 19 20 Telfer A et al. Theridion albidum Banks, 1895 Notes: BOLD:AAV3042 Theridion differens Emerton, 1882 Notes: BOLD:AAC3269 Theridion glaucescens Becker, 1879 Notes: BOLD:AAG1794 Theridion murarium Emerton, 1882 Notes: BOLD:AAC6350 Theridula emertoni Levi, 1954 Notes: BOLD:AAD2291 Thymoites unimaculatus Emerton, 1882 Notes: BOLD:AAE7853 Yunohamella lyrica Walckenaer, 1841 Notes: BOLD:AAG4815 Family Thomisidae Mecaphesa asperata Hentz, 1847 Notes: BOLD:ACE7683 Misumena vatia Clerck, 1757 Notes: BOLD:AAA6275 Misumenoides formosipes Walckenaer, 1837 Misumessus oblongus Keyserling, 1880 Ozyptila americana Banks, 1895 Ozyptila praticola C. L. Koch, 1837 Notes: BOLD:AAC7413 Tmarus angulatus Walckenaer, 1837 Notes: BOLD:ABY7475 Xysticus bicuspis Keyserling, 1887 Notes: BOLD:AAJ9685 Xysticus discursans Keyserling, 1880 Notes: BOLD:AAJ9718|BOLD:ACV2014|BOLD:ACV5078 Xysticus elegans Keyserling, 1880 Notes: BOLD:AAC1568 Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Xysticus emertoni Keyserling, 1880 Notes: BOLD:AAB4300 Xysticus funestus Keyserling, 1880 Xysticus luctans C. L. Koch, 1845 Notes: BOLD:AAF8190 Xysticus punctatus Keyserling, 1880 Notes: BOLD:AAD2346 Xysticus winnipegensis Turnbull, Dondale & Redner, 1965 Notes: BOLD:AAM6956 Family Uloboridae Uloborus glomosus Walckenaer, 1841 Notes: BOLD:AAJ7823 Order Mesostigmata Family Ascidae Arctoseius cetratus Sellnick, 1940 Notes: BOLD:ACF8021 Family Rhodacaridae Rhodacarellus silesiacus Willmann, 1935 Order Opiliones Family Phalangiidae Oligolophus tridens C. L. Koch, 1836 Notes: BOLD:AAM8194 Phalangium opilio Linnaeus, 1758 Notes: BOLD:AAI4346 Platybunus triangularis Herbst, 1799 Notes: BOLD:ABW0506 Family Sclerosomatidae Leiobunum aldrichi Weed, 1893 Notes: BOLD:AAH7061 Leiobunum ventricosum Wood, 1868 Order Sarcoptiformes Family Euzetidae Euzetes globulus Nicolet, 1855 Notes: BOLD:AAF9090 21 22 Telfer A et al. Family Gustaviidae Gustavia microcephala Nicolet, 1855 Notes: BOLD:ACE3149 Family Liacaridae Dorycranosus acutidens Aoki, 1965 Notes: BOLD:AAF9274 Family Mochlozetidae Podoribates pratensis Banks, 1895 Notes: BOLD:AAF9173 Family Mycobatidae Punctoribates punctum Koch, 1839 Notes: BOLD:AAH6516|BOLD:ACB6310 Family Oppiidae Oppia nitens Koch, 1836 Notes: BOLD:AAF0868 Family Oribatulidae Oribatula tibialis Nicolet, 1855 Notes: BOLD:ACI4357 Family Scheloribatidae Scheloribates clavilanceolatus Ewing, 1907 Notes: BOLD:AAF9097 Family Tectocepheidae Tectocepheus sarekensis Trägårdh, 1910 Notes: BOLD:AAM3402|BOLD:AAM4355 Order Trombidiformes Family Arrenuridae Arrenurus reflexus Marshall, 1908 Notes: BOLD:AAE6722 Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Class Branchiopoda Order Diplostraca Family Bosminidae Bosmina liederi De Melo and Hebert, 1994 Family Eurycercidae Eurycercus longirostris Hann, 1982 Class Chilopoda Order Geophilomorpha Family Schendylidae Schendyla nemorensis C.L.Koch, 1837 Notes: BOLD:AAG8560 Order Lithobiomorpha Family Lithobiidae Lithobius microps Meinert, 1868 Notes: BOLD:AAH6432 Class Collembola Entomobrya atrocincta Schott, 1896 Notes: BOLD:ACE5102 Family Entomobryidae Entomobrya nivalis Linnaeus, 1758 Notes: BOLD:ACL6239 Lepidocyrtus paradoxus Uzel, 1891 Orchesella villosa Linnaeus, 1767 Notes: BOLD:AAA8726 Pseudosinella octopunctata Boerner, 1901 Notes: BOLD:AAA9292 Family Isotomidae Parisotoma notabilis Schaffer, 1896 Order Poduromorpha Family Hypogastruridae Ceratophysella bengtssoni Ågren, 1904 Notes: BOLD:AAI3738 23 24 Telfer A et al. Order Symphypleona Family Dicyrtomidae Dicyrtomina minuta Fabricius, 1783 Family Katiannidae Sminthurinus elegans Fitch, 1862 Notes: BOLD:AAB3495|BOLD:AAB3496 Brachyiulus pusillus Leach, 1815 Notes: BOLD:AAM7944 Family Julidae Cylindroiulus caeruleocinctus Wood, 1864 Notes: BOLD:AAH7472 Julus scandinavius Latzel, 1884 Notes: BOLD:AAH7469 Ophyiulus pilosus Newport, 1843 Class Insecta Order Coleoptera Family Anobiidae Caenocara sp. Ptilinus ruficornis Say, 1823 Family Anthicidae Malporus formicarius LaFerté-Sénectère, 1849 Notes: BOLD:ACF8552 Notoxus desertus Casey, 1895 Notes: BOLD:ABX0657 Stricticomus tobias Marseul, 1879 Notes: BOLD:AAQ1028 Family Anthribidae Anthribus nebulosus Forster, 1770 Notes: BOLD:AAO1339 Ormiscus walshii LeConte, 1876 Notes: BOLD:AAU7341 Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Family Buprestidae Agrilus sulciollis Lacordaire, 1835 Family Byrrhidae Simplocaria semistriata Fabricius, 1801 Notes: BOLD:ABW1696 Family Byturidae Byturus unicolor Say, 1823 Family Cantharidae Cantharis rufa Linnaeus, 1758 Chauliognathus pensylvanicus DeGeer, 1774 Rhagonycha fulva Scopoli, 1763 Rhaxonycha carolina Fabricius, 1801 Family Carabidae Agonoleptus conjunctus Say, 1823 Notes: BOLD:AAE9008 Agonum fidele Casey, 1920 Agonum gratiosum Mannerheim, 1853 Amara angustata Say, 1823 Amara rubrica Haldeman, 1843 Notes: BOLD:AAM7658 Bembidion frontale LeConte, 1847 Notes: BOLD:AAU7150 Bembidion obtusum Audinet-Serville, 1821 Notes: BOLD:AAP9490 Calleida punctata LeConte, 1846 Carabus granulatus Linnaeus, 1758 Carabus nemoralis Müller, 1764 Chlaenius tricolor Dejean, 1826 Clivina fossor Linnaeus, 1758 Notes: BOLD:AAH0274 Colliuris pensylvanica Linnaeus, 1758 Dyschirius setosus LeConte, 1857 Elaphrus clairvillei Kirby, 1837 Lebia fuscata Dejean, 1825 Notes: BOLD:AAH0212 25 26 Telfer A et al. Lebia solea Hentz, 1830 Lebia viridis Say, 1823 Notes: BOLD:AAH0141 Platynus hypolithos Say, 1823 Pterostichus melanarius Illiger, 1798 Family Cerambycidae Acalymma vittata Barber, 1947 Astylopsis macula Say, 1826 Astylopsis sexguttata Say, 1826 Bellamira scalaris Say, 1826 Calligrapha californica subsp. coreopsivora Brown Chaetocnema sp. Charidotella sexpunctata subsp. bicolor Fabricius, 1798 Chrysochus auratus Fabricius, 1775 Chrysolina hudsonica Brown, 1938 Cicindela punctulata subsp. punctulata Olivier Cicindela sexguttata Fabricius, 1775 Clytus ruricola Olivier, 1795 Crepidodera sp. Cyrtophorus verrucosus Olivier, 1795 Notes: BOLD:AAD4513 Deloyala guttata Olivier, 1790 Dibolia sp. Epitrix sp. Euderces picipes Fabricius, 1787 Gaurotes cyanipennis Say, 1824 Notes: BOLD:AAI7042 Longitarsus sp. Mantura floridana Crotch, 1873 Megacyllene robiniae Forster, 1771 Molorchus bimaculatus Say, 1824 Notes: BOLD:AAH0019 Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Neoclytus acuminatus subsp. acuminatus Fabricius Oberea tripunctata Swederus, 1787 Obrium rufulum Gahan, 1908 Oulema palustris Blatchley, 1913 Paria sp. Phyllotreta sp. Phymatodes amoenus Say, 1824 Plagiodera versicolor Laicharting, 1781 Psylliodes punctulata Melsheimer, 1847 Pyrrhalta sp. Systena blanda F. E. Melsheimer, 1847 Tetraopes tetrophthalmus Forster, 1771 Tetrops praeusta Linnaeus, 1758 Notes: BOLD:AAE9431 Trigonarthris proxima Say, 1824 Trirhabda sp. Typocerus velutinus subsp. velutinus Olivier Urgleptes querci Fitch, 1858 Xylotrechus convergens LeConte, 1873 Xylotrechus gemellus Casey, 1893 Zeugophora varians Crotch, 1873 Family Cerylonidae Philothermus glabriculus LeConte, 1863 Notes: BOLD:ABX9329 Family Chrysomelidae Altica chalybea Illiger, 1807 Altica sp. Cassida rubiginosa Müller, 1776 Notes: BOLD:AAO0522 Crepidodera heikertingeri Lazorko, 1974 Notes: BOLD:AAG4462 Dibolia borealis Chevrolat in Guérin-Méneville, 1834 Notes: BOLD:AAL0908 Epitrix cucumeris Harris, 1851 Notes: BOLD:ABA9101 27 28 Telfer A et al. Exema canadensis Pierce, 1940 Helocassis clavata Fabricius, 1798 Microrhopala vittata Fabricius, 1798 Neogalerucella calmariensis Linnaeus, 1767 Notes: BOLD:AAL2945 Ophraella conferta J. L. LeConte, 1865 Notes: BOLD:ACF8270 Paria fragariae Wilcox, 1954 Notes: BOLD:ABA3960|BOLD:ACF6671 Phyllotreta striolata Fabricius, 1801 Notes: BOLD:AAL5267 Plagiodera versicolora Laicharting, 1781 Psylliodes affinis Paykull, 1799 Notes: BOLD:AAU6967 Psylliodes napi Fabricius, 1792 Psylliodes picinus Marsham, 1802 Pyrrhalta viburni Paykull, 1799 Trirhabda borealis Blake, 1931 Notes: BOLD:AAG4458 Xanthonia decemnotata Say, 1824 Notes: BOLD:ABA6335|BOLD:ACJ0239 Family Ciidae Ceracis thoracicornis Ziegler, 1845 Family Cleridae Cymatodera bicolor Say, 1825 Notes: BOLD:AAU6910 Enoclerus nigripes Say, 1823 Notes: BOLD:AAU6970 Enoclerus rosmarus Say, 1823 Isohydnocera curtipennis Newman Phyllobaenus verticalis Say, 1835 Placopterus thoracicus Olivier, 1795 Notes: BOLD:AAP8584 Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Zenodosus sanguineus Say, 1835 Notes: BOLD:ABA6311 Family Coccinellidae Coccinella septempunctata Linnaeus, 1758 Coleomegilla maculata De Geer, 1775 Notes: BOLD:AAD7604 Coleomegilla maculata subsp. lengi Timberlake Didion punctatum Melsheimer, 1847 Harmonia axyridis Pallas, 1773 Notes: BOLD:AAB5640 Hippodamia glacialis Fabricius, 1775 Notes: BOLD:AAH3305 Hippodamia variegata Goeze, 1777 Hyperaspis binotata Say, 1826 Hyperaspis cf. binotata Propylaea quatuordecimpunctata Linnaeus, 1758 Notes: BOLD:AAF6935 Propylea quatuordecimpunctata Linnaeus, 1758 Psyllobora vigintimaculata subsp. maculata Say Scymnus sp. Stethorus punctillum Weise, 1891 Notes: BOLD:AAN6149 Family Corylophidae Orthoperus scutellaris LeConte, 1878 Notes: BOLD:AAU7040 Sericoderus lateralis Gyllenhal, 1827 Notes: BOLD:ABA2914 Family Cryptophagidae Atomaria ephippiata Zimmermann, 1869 Family Curculionidae Acoptus suturalis LeConte, 1876 Notes: BOLD:AAU6930 Barypeithes pellucidus Boheman, 1834 Notes: BOLD:AAG5192 29 30 Telfer A et al. Hylesinus aculeatus Say, 1824 Notes: BOLD:AAU7331 Hypera zoilus Scopoli, 1763 Isochnus sequensi Stierlin 1894 Notes: BOLD:ACA3052 Madarellus undulatus Say, 1824 Notes: BOLD:AAY6533 Monarthrum mali Fitch, 1855 Notes: BOLD:ACD0202 Orchestes alni Linnaeus 1758 Notes: BOLD:AAM7726 Phyllobius oblongus Linnaeus, 1758 Notes: BOLD:AAF9187 Pityogenes hopkinsi Swaine, 1915 Notes: BOLD:ABW5076 Polydrusus impressifrons Gyllenhal, 1834 Notes: BOLD:AAO4332 Sitona lineellus Bonsdorff, 1785 Tychius meliloti Stephens, 1831 Notes: BOLD:AAM7740 Xyleborinus alni Niijima 1909 Notes: BOLD:AAB2754 Xyleborinus saxeseni Ratzeburg, 1837 Notes: BOLD:AAB9578 Xyleborus dispar Fabricius, 1792 Notes: BOLD:AAD0158 Xylosandrus germanus Blandford, 1894 Notes: BOLD:AAF7523 Family Dytiscidae Desmopachria convexa Aubé, 1838 Family Elateridae Aeolus mellillus Say, 1836 Ampedus areolatus Say, 1823 Notes: BOLD:ACM2015 Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Ampedus linteus Say, 1839 Notes: BOLD:AAU7141 Ampedus nigricollis Herbst, 1801 Notes: BOLD:AAH2376 Ampedus oblessus Say, 1833 Notes: BOLD:ACA3849 Ampedus protervus LeConte, 1853 Notes: BOLD:ACR3975 Athous brightwelli Kirby, 1837 Corymbitodes tarsalis Melsheimer, 1844 Notes: BOLD:ACV5201 Ctenicera cylindriformis Herbst, 1806 Notes: BOLD:AAH2370 Dalopius vagus Brown, 1934 Hemicrepidius memnonius Herbst, 1806 Melanotus castanipes Paykull, 1800 Notes: BOLD:AAH2378 Family Endomychidae Mycetina perpulchra Newman, 1838 Phymaphora pulchella Newman, 1838 Notes: BOLD:ACI7114 Family Erotylidae Triplax flavicollis Lacordaire, 1842 Triplax thoracica Say, 1825 Tritoma pulchra Say, 1826 Tritoma sanguinipennis Say, 1825 Family Eucnemidae Dirrhagofarsus lewisi Fleutiaux, 1900 Hylis terminalis LeConte, 1866 Isorhipis obliqua Say, 1836 Microrhagus sp. 31 32 Telfer A et al. Microrhagus subsinuata LeConte Microrhagus triangularis Say, 1823 Family Gyrinidae Dineutus assimilis Kirby, 1937 Family Haliplidae Haliplus immaculicollis Harris, 1828 Family Hydrophilidae Anacaena lutescens Stephens, 1829 Cercyon haemorrhoidalis Fabricius, 1775 Notes: BOLD:ABV1545 Enochrus ochraceus Melsheimer, 1844 Tropisternus natator Orchymont, 1938 Family Kateretidae Brachypterolus pulicarius Linnaeus, 1758 Family Lampyridae Ellychnia corrusca Linnaeus, 1767 Notes: BOLD:ACV4844 Lucidota atra G. Olivier, 1790 Photinus sp. Pyractomena sp. Pyropyga nigricans Say, 1823 Family Languriidae Acropteroxys gracilis Newman 1838 Family Latridiidae Cortinicara gibbosa Herbst, 1793 Notes: BOLD:AAI8935 Family Leiodidae Anisotoma obsoleta Horn, 1880 Notes: BOLD:AAR3435 Anisotoma sp. Catops paramericanus Peck and Cook, 2002 Notes: BOLD:AAH3504 Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Catops sp. Lionothus sp. Nemadus sp. Prionochaeta opaca Say, 1825 Notes: BOLD:AAP6949 Ptomophagus sp. Sciodrepoides fumatus subsp. terminans LeConte Family Melandryidae Dircaea liturata LeConte, 1866 Epicauta pensylvanica De Geer, 1775 Melandrya striata Say, 1824 Notes: BOLD:AAK7242 Family Meloidae Epicauta murina LeConte, 1853 Meloe impressus Kirby, 1837 Family Melyridae Collops quadrimaculata Fabricius, 1798 Hypebaeus apicalis Say, 1825 Notes: BOLD:AAN5932 Family Mordellidae Mordellina infima LeConte, 1862 Mordellistena andreae LeConte, 1862 Mordellistena bifasciata Ray, 1936 Mordellistena cervicalis LeConte, 1862 Mordellistena cf. lutea Mordellistena ornata Melsheimer, 1845 Mordellistena picilabris Helmuth, 1864 Mordellistena sp. Mordellochroa scapularis Say, 1824 Notes: BOLD:AAU6912 33 34 Telfer A et al. Paramordellaria triloba Say, 1824 Tomoxia lineela LeConte Family Mycetophagidae Mycetophagus pluripunctatus LeConte, 1856 Family Nitidulidae Carpophilus sayi Parsons, 1943 Conotelus obscurus Erichson, 1843 Glischrochilus fasciatus Olivier, 1790 Glischrochilus quadrisiginatus Say 1835 Glischrochilus sanguinolentus subsp. sanguinolentus Olivier, 1790 Omosita colon Linnaeus, 1758 Stelidota octomaculata Say, 1825 Notes: BOLD:AAH0115 Family Pedilidae Pedilus lugubris Say Family Phalacridae Acylomus pugetanus Casey, 1916 Notes: BOLD:AAH0135 Olibrus semistriatus LeConte, 1856 Stilbus apicialis Melsheimer, 1844 Notes: BOLD:AAH0134 Family Psephenidae Psephenus herricki DeKay, 1844 Family Ptilodactylidae Ptilodactyla sp. Family Ptinidae Hadrobregmus notatus Say, 1825 Notes: BOLD:AAP8586 Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Family Pyrochroidae Dendroides canadensis Leconte Schizotus cervicalis Newman, 1838 Family Rhipiphoridae Pelecotoma flavipes Melsheimer, 1846 Ripiphorus fasciatus Say, 1824 Family Scarabaeidae Amphimallon majale Razumowski, 1789 Aphodius granarius Linnaeus, 1767 Ataenius strigatus Say, 1823 Calamosternus granarius Linnaeus, 1767 Notes: BOLD:AAM7733 Onthophagus orpheus subsp. canadensis Fabricius, 1801 Osmoderma scabra Palisot de Beauvois, 1805 Phyllophaga futilis LeConte, 1850 Notes: BOLD:AAD1098 Phyllophaga rugosa Melsheimer, 1845 Notes: BOLD:AAJ2312 Popillia japonica Newman, 1841 Rhyssemus germanus Linnaeus, 1767 Trox scabrosus Beauvois, 1805 Family Scirtidae Cyphon laevipennis Tournier, 1868 Notes: BOLD:AAG3633 Cyphon obscurus Guerin and Memeville, 1834 Notes: BOLD:AAG7259 Cyphon pusillus LeConte Notes: BOLD:AAP7021 35 36 Telfer A et al. Scirtes tibialis Guerin Family Scolytidae Hylurgopinus rufipes Eichhoff, 1868 Scolytus mali Bechstein, 1805 Xyleborus sayi Hopkins, 1915 Family Scraptiidae Anaspis rufa Say, 1826 Notes: BOLD:AAH0469 Canifa pallipes Melsheimer, 1846 Family Silphidae Necrophila americana Linnaeus, 1758 Nicrophorus orbicollis Say, 1825 Notes: BOLD:AAE1939 Oiceoptoma inaequale Fabricius, 1781 Family Silvanidae Ahasverus advena Waltl, 1834 Notes: BOLD:AAJ2005 Silvanus bidentatus Fabricius, 1792 Notes: BOLD:AAO0157 Telephanus velox Haldeman, 1846 Notes: BOLD:AAW6380 Family Staphylinidae Amischa analis Gravenhorst, 1802 Notes: BOLD:ABA5313 Anotylus insecatus Gravenhorst, 1806 Notes: BOLD:AAR3352 Anotylus tetracarinatus Block, 1799 Atheta brunneipennis Thomson, 1852 Notes: BOLD:ABA9094 Bisnius blandus Gravenhorst, 1806 Carpelimus fuliginosus Gravenhorst, 1802 Notes: BOLD:AAO0558 Coproporus ventriculus Say, 1834 Notes: BOLD:ACV1788 Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Lordithon appalachianus Campbell, 1982 Notes: BOLD:ABA6331 Lordithon cinctus Gravenhorst, 1802 Notes: BOLD:ABA6370 Meronera venustula Erichson, 1839 Notes: BOLD:ABW2870 Myllaena arcana Casey, 1911 Notes: BOLD:ACJ6804 Philonthus caeruleipennis Mannerheim, 1830 Philonthus flavibasis Casey, 1915 Notes: BOLD:AAH0113 Platydracus cinnamopterus Gravenhorst, 1802 Notes: BOLD:ACJ0017 Scaphidium quadriguttatum Melsheimer Notes: BOLD:ACP0011 Sepedophilus cinctulus Erichson, 1839 Notes: BOLD:ACC1294 Sepedophilus testaceus Fabricius, 1793 Notes: BOLD:AAH0108 Stenichnus scutellaris Muller & Kunze, 1822 Notes: BOLD:AAN9916 Tachinus corticinus Gravenhorst, 1802 Notes: BOLD:AAH0107 Tachyporus atriceps Stephens, 1832 Notes: BOLD:ABX2484 Tachyporus chrysomelinus Linnaeus, 1758 Notes: BOLD:AAN9511 Tachyporus elegans Horn, 1877 Notes: BOLD:AAU6934 Tachyporus nitidulus Fabricius, 1781 Notes: BOLD:ABA9096 Trichophya pilicornis Gyllenhal, 1810 Notes: BOLD:ABW9580 37 38 Telfer A et al. Xantholinus linearis Olivier, 1795 Notes: BOLD:AAG4333 Family Stenotrachelidae Cephaloon lepturoides Newman, 1838 Family Synchroidae Mallodrya subaenea Horn, 1888 Notes: BOLD:AAK7440 Synchroa punctata Newman, 1838 Family Tetratomidae Eustrophus tomentosus Say, 1827 Notes: BOLD:ACI7017 Family Throscidae Aulonothroscus constrictor Say, 1839 Notes: BOLD:AAU7339 Aulonothroscus distans Blanchard, 1917 Notes: BOLD:ABA9083 Aulonothroscus sp. Trixagus carinicollis Schaeffer, 1916 Notes: BOLD:AAN6148 Trixagus chevrolati Bonvouloir, 1859 Family Trogossitidae Tenebroides corticalis Melsheimer, 1844 Order Dermaptera Family Forficulidae Forficula auricularia Linnaeus, 1758 Forficula auricularia-A Linnaeus, 1758 Notes: BOLD:AAG9897 Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... 39 Checklist of species observed or collected at the rare Charitable Research Reserve in Cambridge, Ontario, Canada. The second of five checklists for Kingdom Animalia, this checklist contains members of Phylum Arthopoda, Class Insecta (Orders Diptera, Ephemeroptera, and Hemiptera). Phylum Arthropoda Kingdom Animalia Class Insecta Order Diptera Family Agromyzidae Agromyza frontella Rondani, 1874 Notes: BOLD:AAJ7105 Aulagromyza luteoscutellata de Meijere, 1924 Notes: BOLD:AAJ9681 Calycomyza majuscula Frick, 1956 Notes: BOLD:AAV4861 Cerodontha biseta Hendel, 1920 Cerodontha dorsalis Loew, 1863 Cerodontha fasciata Strobl, 1880 Cerodontha muscina Meigen, 1830 Notes: BOLD:AAF1051 Chromatomyia lactuca Frost, 1924 Japanagromyza viridula Coquillett, 1902 Notes: BOLD:AAI7960 Liriomyza brassicae Riley, 1885 Notes: BOLD:AAF6806 Liriomyza fricki Spencer, 1965 Notes: BOLD:ACE7414 Nemorimyza posticata Meigen, 1830 Notes: BOLD:AAG9234|BOLD:ACJ0616 40 Telfer A et al. Ophiomyia labiatarum Hering, 1937 Ophiomyia nasuta Melander, 1913 Notes: BOLD:AAK5607 Ophiomyia quinta Spencer, 1969 Notes: BOLD:AAI3360|BOLD:ABZ1036 Ophiomyia similata Malloch, 1918 Notes: BOLD:AAP8823|BOLD:ACV3095|BOLD:ACV5944 Phytoliriomyza dorsata Siebke, 1864 Notes: BOLD:AAG4751 Phytoliriomyza robiniae Valley, 1982 Notes: BOLD:AAY1337 Phytomyza flavicornis Fallen, 1823 Notes: BOLD:AAH9376 Phytomyza solidaginophaga Sehgal, 1971 Notes: BOLD:AAL4176 Pseudonapomyza europaea Spencer, 1973 Family Anisopodidae Sylvicola alternatus Say, 1823 Notes: BOLD:AAG2000 Sylvicola fuscatus Fabricius, 1775 Notes: BOLD:AAG1998 Family Anthomyiidae Anthomyia pluvialis Linnaeus, 1758 Notes: BOLD:AAP2970 Delia antiqua Meigen, 1826 Delia platura Meigen, 1826 Notes: BOLD:AAA3453 Eustalomyia festiva Zetterstedt, 1845 Notes: BOLD:AAP8833 Eutrichota pilimana Ringdahl, 1918 Notes: BOLD:AAP2968 Hylemyza partita Meigen, 1826 Notes: BOLD:AAG2463 Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Pegomya flavifrons Walker, 1849 Notes: BOLD:AAG2479 Family Anthomyzidae Mumetopia occipitalis Melander, 1913 Notes: BOLD:AAG4827 Stiphrosoma balteatum Rohacek & Barber, 2005 Family Asilidae Dioctria baumhaueri Meigen, 1820 Efferia aestuans Linnaeus, 1763 Efferia albibarbis Macquart, 1838 Laphria canis Williston, 1883 Laphria canis complex Williston, 1883 Laphria cinerea Back, 1904 Laphria flavicollis Say, 1824 Laphria janus McAtee, 1919 Laphria sicula McAtee, 1919 Laphria thoracica Fabricius, 1805 Machimus sadyates Walker, 1849 Family Aulacigastridae Aulacigaster neoleucopeza Mathis & Freidberg, 1994 Notes: BOLD:AAV0437|BOLD:ABV3853 Family Bombyliidae Anthrax irroratus Say, 1823 Bombylius major Linnaeus, 1758 Notes: BOLD:ABV0388 Hemipenthes morio Linnaeus, 1758 Hemipenthes sinuosa Wiedemann, 1821 Hemipenthes webberi Johnson, 1919 Xenox tigrinus De Geer, 1776 Family Calliphoridae Calliphora livida Hall, 1948 Notes: BOLD:ABY7153 Cynomya cadaverina Robineau-Desvoidy, 1830 Notes: BOLD:AAB0868 41 42 Telfer A et al. Phormia regina Meigen, 1826 Notes: BOLD:AAB9140 Pollenia angustigena Wainwright, 1940 Notes: BOLD:AAP2825 Pollenia griseotomentosa Jacentkovsky, 1944 Notes: BOLD:AAI2766 Pollenia labialis Robineau-Desvoidy, 1863 Notes: BOLD:AAI2765 Pollenia pediculata Macquart, 1834 Notes: BOLD:AAG6745 Pollenia rudis Fabricius, 1794 Notes: BOLD:AAH3035 Family Cecidomyiidae Asteromyia carbonifera Osten Sacken, 1862 Notes: BOLD:AAA2254|BOLD:ABX5689 Asteromyia laeviana Felt, 1907 Notes: BOLD:ABV1420 Asteromyia modesta Felt, 1907 Notes: BOLD:AAM1947|BOLD:AAM1948|BOLD:AAM1954|BOLD:ACG8775|B OLD:ACN2213 Asteromyia tumifica Beutenmuller, 1907 Notes: BOLD:ACL0470 Janetiella glechomae Tavares, 1930 Notes: BOLD:AAQ0642 Mayetiola destructor Say, 1817 Notes: BOLD:ABV9277 Family Ceratopogonidae Stilobezzia antennalis Coquillett, 1901 Family Chironomidae Bryophaenocladius ictericus Meigen, 1830 Notes: BOLD:AAM6273 Bryophaenocladius sp. 8ES Notes: BOLD:AAG1021 Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Camptocladius stercorarius De Geer, 1776 Notes: BOLD:AAN5341 Chironomus acidophilus Keyl, 1960 Notes: BOLD:AAJ4295 Chironomus dilutus Kiknadze & Butler, 1999 Notes: BOLD:AAB4658 Chironomus maturus Johannsen, 1908 Notes: BOLD:AAB4657 Chironomus melanescens Keyl, 1961 Notes: BOLD:AAI4303 Chironomus ochreatus Townes, 1945 Notes: BOLD:ACV5571 Cladotanytarsus atridorsum Kieffer, 1924 Notes: BOLD:AAJ3263 Conchapelopia telema Roback, 1971 Notes: BOLD:AAC4802|BOLD:AAN5351 Corynoneura scutellata Winnertz, 1846 Notes: BOLD:AAN5330 Cricotopus annulator cmplx Goetghebuer, 1927 Cricotopus bicinctus Meigen, 1818 Notes: BOLD:ACC7282 Cricotopus sp. 18ES Cricotopus sp. 19ES Notes: BOLD:AAP5141 Cricotopus tremulus Linnaeus, 1758 Notes: BOLD:AAE4298 Cricotopus triannulatus Macquart, 1826 Notes: BOLD:AAP5920 Cricotopus trifascia Edwards, 1929 Notes: BOLD:ACS9429|BOLD:ACT0257 Cricotopus vierriensis Goetghebuer, 1935 Notes: BOLD:AAG1005|BOLD:ACV5403|BOLD:ACV5404 43 44 Telfer A et al. Dicrotendipes modestus Say, 1823 Notes: BOLD:AAL7329 Dicrotendipes tritomus Kieffer, 1916 Notes: BOLD:AAC0706 Gymnometriocnemus brumalis Edwards, 1929 Notes: BOLD:AAP6873 Lauterborniella agrayloides Kieffer, 1911 Notes: BOLD:AAN5343 Limnophyes natalensis Kieffer, 1914 Notes: BOLD:AAB7361 Limnophyes sp. 14ES Notes: BOLD:ABU5525 Metriocnemus sp. 4ES Notes: BOLD:ABX5809 Micropsectra nigripila Johannsen, 1905 Micropsectra subletteorum Anderson, Stur & Ekrem, 2013 Notes: BOLD:AAF7088 Microtendipes pedellus De Geer, 1776 Notes: BOLD:AAE0707 Monopelopia tenuicalcar Kieffer, 1918 Notes: BOLD:AAM6277 Nanocladius anderseni Saether, 1977 Notes: BOLD:AAC3041 Nilotanypus fimbriatus Walker, 1828 Notes: BOLD:AAE5762 Orthocladius carlatus Roback, 1957 Notes: BOLD:AAG1000 Orthocladius dorenus Roback, 1957 Notes: BOLD:AAB2641|BOLD:AAB2645|BOLD:ACV3368 Orthocladius oliveri Soponis, 1977 Notes: BOLD:AAB7872 Orthocladius rivulorum Kieffer, 1909 Notes: BOLD:AAB3988 Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Pagastia orthogonia Oliver, 1959 Notes: BOLD:AAI2601 Paraphaenocladius impensus Walker, 1856 Notes: BOLD:AAC4197 Paratanytarsus dissimilis Johannsen, 1905 Notes: BOLD:AAE3698 Paratanytarsus grimmii Schneider, 1885 Notes: BOLD:AAD1485 Paratanytarsus laccophilus Edwards, 1929 Notes: BOLD:AAC8842|BOLD:ACF2457 Paratanytarsus sp. 7TE Notes: BOLD:AAP2907 Paratanytarsus sp. TE03 Notes: BOLD:AAE3675 Polypedilum convictum Walker, 1856 Notes: BOLD:AAD1397 Psectrocladius obvius Walker, 1856 Notes: BOLD:AAF6432 Rheocricotopus robacki Beck and Beck, 1964 Notes: BOLD:AAM6249 Rheotanytarsus pellucidus Walker, 1848 Notes: BOLD:AAI0332 Smittia edwardsi Goetghebuer, 1932 Notes: BOLD:AAF4817 Smittia sp. 14ES Notes: BOLD:AAM7064 Smittia sp. 22ES Notes: BOLD:AAN5358 Smittia sp. 23ES Notes: BOLD:AAH9641 Smittia sp. 8ES Notes: BOLD:ACP4736 45 46 Telfer A et al. Stempellinella fimbriata Ekrem, 2007 Notes: BOLD:AAD0300 Tanytarsus glabrescens Edwards, 1929 Notes: BOLD:ACV5604|BOLD:ACV5898 Tanytarsus guerlus Roback, 1957 Notes: BOLD:AAC4523|BOLD:AAC4525 Tanytarsus mendax Kieffer, 1925 Notes: BOLD:ACJ3722|BOLD:ACV3832 Tanytarsus recurvatus Brundin, 1947 Notes: BOLD:AAC3354 Tanytarsus wirthi Ekrem, Sublette & Sublette, 2003 Notes: BOLD:AAD2144 Thienemanniella xena Roback, 1957 Notes: BOLD:AAD5253|BOLD:AAD5254 Family Chloropidae Chaetochlorops inquilinus Coquillett, 1898 Elachiptera costata Loew, 1863 Elachiptera nigriceps Loew, 1863 Notes: BOLD:AAP5169 Elachiptera sibirica Loew, 1858 Notes: BOLD:AAH4208 Eribolus longulus Loew, 1863 Gaurax dubius Macquart, 1835 Notes: BOLD:ACC7744 Gaurax pallidipes Malloch, 1915 Notes: BOLD:AAH4210 Gaurax varihalteratus Malloch, 1913 Notes: BOLD:ACM2340 Hapleginella conicola Greene, 1918 Malloewia abdominalis Becker, 1912 Notes: BOLD:ABW1379 Malloewia nigripalpis Malloch, 1913 Notes: BOLD:ABZ4644 Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Olcella provocans Becker, 1912 Oscinella frit Linnaeus, 1758 Oscinisoma alienum Becker, 1912 Notes: BOLD:ACE3223 Psilacrum arpidia Malloch, 1916 Notes: BOLD:ACE0829 Rhopalopterum carbonarium Loew, 1869 Thaumatomyia glabra Meigen, 1830 Notes: BOLD:AAH4135 Family Chyromyidae Gymnochiromyia concolor Malloch, 1914 Notes: BOLD:ACV5890 Family Clusiidae Clusia czernyi Johnson, 1913 Notes: BOLD:AAF4394 Clusia lateralis Walker, 1849 Clusiodes johnsoni Malloch, 1922 Notes: BOLD:AAJ4032 Clusiodes melanostomus Loew, 1864 Notes: BOLD:AAJ4031 Sobarocephala flaviseta Johnson, 1913 Notes: BOLD:AAN5648 Sobarocephala setipes Melander and Argo, 1924 Family Conopidae Physocephala marginata Say, 1823 Family Culicidae Aedes canadensis Theobald Aedes cinereus Meigen, 1818 Notes: BOLD:AAC1222 Aedes sp. Aedes stimulans (Walker, 1848) Aedes vexans Meigen, 1830 Notes: BOLD:AAA7067 47 48 Telfer A et al. Anopheles quadrimaculatus Say, 1824 Coquillettidia perturbans Walker, 1856 Notes: BOLD:AAB2539 Culex restuans Theobald, 1901 Notes: BOLD:AAA7661 Culex territans Walker, 1856 Notes: BOLD:AAB6943|BOLD:ABY7666 Mansonia perturbans Walker Family Dolichopodidae Dolichopus orichalceus Gosseries, 1989 Dolichopus terminalis Loew, 1866 Gymnopternus celer Meigen, 1824 Medetera signaticornis Loew, 1857 Notes: BOLD:AAZ3931 Neurigona disjuncta Van Duzee, 1913 Notes: BOLD:ABW1193 Xanthochlorus helvinus Loew, 1861 Family Drosophilidae Chymomyza amoena Loew, 1862 Notes: BOLD:AAE2703 Drosophila affinis Sturtevant, 1916 Notes: BOLD:AAB8851 Drosophila falleni Wheeler, 1960 Notes: BOLD:AAB7507 Leucophenga varia Walker, 1849 Notes: BOLD:AAG8500 Scaptomyza adusta Loew, 1862 Notes: BOLD:AAG8491 Family Empididae Rhamphomyia versicolor Chillcott, 1959 Notes: BOLD:AAM7337 Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Family Ephydridae Athyroglossa dinorata Mathis & Zatwarnicki, 1990 Athyroglossa granulosa Cresson, 1922 Notes: BOLD:ABY0801 Axysta extera Cresson, 1942 Coenia curvicauda Meigen, 1830 Discocerina obscurella Fallen, 1813 Discomyza incurva Fallen 1823 Notes: BOLD:ABA8754 Hyadina albovenosa Coquillett, 1900 Hydrellia albilabris Meigen, 1830 Hydrellia griseola Fallen, 1813 Hydrellia ischiaca Loew, 1862 Hydrellia notata Deonier, 1971 Nostima scutellaris Cresson, 1933 Ochthera anatolikos Clausen, 1977 Parydra aquila Fallen, 1813 Philygria debilis Loew, 1861 Philygria obtecta Becker, 1896 Notes: BOLD:AAG2740 Pseudohyadina longicornis Sturtevant and Wheeler, 1954 Scatella favillacea Loew, 1862 Scatella stagnalis Fallen, 1813 Scatella tenuicosta Collin, 1930 Scatophila despecta Haliday, 1839 Scatophila virildella Sturtevant & Wheeler, 1954 Family Fanniidae Fannia armata Meigen, 1826 Notes: BOLD:AAU6630 Family Heleomyzidae Suillia quinquepunctata Say, 1823 Notes: BOLD:AAC8595 Family Hybotidae Leptopeza flavipes Meigen, 1820 Notes: BOLD:ACE5974 49 50 Telfer A et al. Platypalpus annulatus Fallen, 1815 Platypalpus holosericus Melander, 1924 Notes: BOLD:AAP6357 Platypalpus melleus Melander, 1927 Notes: BOLD:AAV3697 Platypalpus niger Meigen, 1804 Platypalpus pulicarius Meigen, 1830 Notes: BOLD:AAQ0265 Platypalpus stabilis Collin, 1961 Platypalpus unguiculatus Zetterstedt, 1838 Notes: BOLD:ABA0579 Tachydromia aemula Loew, 1864 Notes: BOLD:AAN5500 Family Keroplatidae Orfelia nemoralis Meigen, 1818 Notes: BOLD:AAP2528 Family Lauxaniidae Lauxania shewelli Perusse & Wheeler 2000 Poecilominettia puncticeps Coquillett, 1902 Notes: BOLD:AAN8633 Family Limoniidae Epiphragma fasciapenne Say, 1823 Notes: BOLD:ACL8650 Erioptera caliptera Say, 1823 Notes: BOLD:AAN5882 Erioptera ebenina Alexander, 1926 Notes: BOLD:ACB0353 Helius flavipes Macq. Notes: BOLD:AAF9008 Ilisia venusta Osten Sacken, 1860 Ormosia affinis Lundbeck, 1898 Notes: BOLD:AAU6544 Ormosia meigenii Osten Sacken, 1859 Notes: BOLD:ACA9818 Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Pseudolimnophila inornata Osten Sacken, 1869 Notes: BOLD:AAI1351 Family Lonchopteridae Lonchoptera bifurcata Fallen, 1810 Family Micropezidae Compsobata univitta Walker, 1849 Notes: BOLD:AAP8989 Rainieria antennaepes Say, 1823 Family Milichiidae Leptometopa latipes Meigen, 1830 Notes: BOLD:AAP8985 Paramyia nitens Loew, 1869 Notes: BOLD:AAG0166|BOLD:AAG0169 Pholeomyia indecora Loew, 1869 Family Muscidae Coenosia tigrina Fabricius, 1775 Helina depuncta Fallen, 1825 Helina evecta Harris, 1780 Notes: BOLD:AAC2498 Helina rufitibia Stein, 1898 Notes: BOLD:AAG1742 Lispe albitarsis Stein, 1898 Notes: BOLD:AAP1125 Lispocephala erythrocera (Robineau-Desvoidy, 1830) Macrorchis ausoba Walker, 1849 Muscina levida Harris, 1788 Notes: BOLD:AAB8817 Myospila meditabunda Fabricius, 1781 Notes: BOLD:AAD7145 Family Mycetophilidae Aglaomyia gatineau Vockeroth, 1980 Notes: BOLD:ABV3010 Exechia attrita Johannsen, 1912 Notes: BOLD:ACM3454 51 52 Telfer A et al. Mycetophila caudata Staeger, 1840 Notes: BOLD:AAI3260 Mycetophila fungorum De Geer, 1776 Notes: BOLD:ACF2821 Mycetophila ocellus Walker, 1848 Notes: BOLD:AAP4734 Paratinia recurva Johannsen, 1910 Symmerus lautus Loew, 1869 Trichonta submaculata (Staeger, 1840) Notes: BOLD:AAU4912 Zygomyia zaitzevi Chandler, 1991 Family Odiniidae Odinia betulae Sabrosky, 1959 Notes: BOLD:AAP8071 Odinia meijerei Collin, 1952 Notes: BOLD:ACV3828 Family Opomyzidae Geomyza apicalis (Meigen, 1830) Notes: BOLD:ACM2703 Geomyza tripunctata Fallen, 1823 Family Pediciidae Pedicia inconstans Osten Sacken, 1859 Family Phoridae Conicera dauci Meigen, 1830 Notes: BOLD:AAN8685 Megaselia arcticae Disney, 2004 Notes: BOLD:AAG3248 Megaselia citrinella Buck & Disney, 2001 Megaselia fungivora Wood, 1909 Megaselia lucifrons (Schmitz, 1918) Notes: BOLD:AAL9075 Megaselia nigriceps Loew, 1866 Notes: BOLD:AAY6384 Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Megaselia rufipes Meigen, 1804 Notes: BOLD:AAG3274 Megaselia variana Schmitz, 1926 Notes: BOLD:AAZ6701 Family Pipunculidae Pipunculus hertzogi Rapp, 1943 Family Psilidae Loxocera cylindrica Say, 1823 Psila lateralis Loew, 1860 Notes: BOLD:AAF9707 Psila persimilis Wakerly, 1959 Psila rosae Fabricius, 1794 Notes: BOLD:AAP6388 Family Psychodidae Psychoda trinodulosa Tonnoir, 1922 Notes: BOLD:AAN8770 Family Rhagionidae Bolbomyia nana Loew, 1862 Notes: BOLD:ACV5660 Chrysopilus thoracicus Fabricius, 1805 Rhagio tringarius Linnaeus, 1758 Family Sarcophagidae Boettcheria bisetosa Parker, 1914 Notes: BOLD:AAH7139 Boettcheria cimbicis Townsend, 1892 Sarcophaga subvicina Baranov, 1937 Notes: BOLD:AAG6743 Senotainia trilineata Wulp, 1890 Notes: BOLD:AAG6744 Family Scathophagidae Americina adusta Loew, 1863 Notes: BOLD:AAH4235 Megaphthalma pallida Fallen, 1819 Notes: BOLD:AAH4234 53 54 Telfer A et al. Parallelomma vittatum Meigen, 1826 Scathophaga furcata Say, 1823 Notes: BOLD:AAH0022 Family Sciaridae Bradysia difformis Frey, 1948 Notes: BOLD:AAV1295 Bradysia fenestralis Zetterstedt, 1838 Notes: BOLD:AAV1366 Bradysia nitidicollis Meigen, 1818 Bradysia pallipes Fabricius, 1787 Notes: BOLD:AAM9254 Bradysia scabricornis Tuomikoski, 1960 Notes: BOLD:ABA0929 Bradysia vagans Winnertz, 1868 Notes: BOLD:AAM9252 Camptochaeta uniformis Mohrig & Menzel, 1990 Notes: BOLD:ACA4924 Corynoptera bicuspidata Lengersdorf, 1926 Notes: BOLD:AAU6513 Corynoptera cuniculata Lengersdorf, 1942 Notes: BOLD:AAU6537 Corynoptera melanochaeta Mohrig & Menzel, 1992 Notes: BOLD:AAM9242 Corynoptera saccata Tuomikoski, 1960 Notes: BOLD:AAN6437 Corynoptera subcavipes Menzel & Smith, 2007 Notes: BOLD:AAU6542 Cratyna ambigua Lengersdorf, 1934 Notes: BOLD:AAH3968|BOLD:AAN6439 Ctenosciara hyalipennis Meigen, 1804 Notes: BOLD:AAH3983 Leptosciarella scutellata Staeger, 1840 Notes: BOLD:ACD1218 Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Lycoriella castanescens Lengersdorf, 1940 Notes: BOLD:ABA1215 Lycoriella perochaeta Mohrig & Menzel, 1990 Notes: BOLD:ACC1855 Lycoriella stylata Mohrig & Mamaev, 1985 Notes: BOLD:AAN6430 Scatopsciara atomaria Zetterstedt, 1851 Notes: BOLD:AAN6431 Sciara humeralis Zetterstedt, 1851 Family Sciomyzidae Poecilographa decorum Loew, 1864 Pteromicra similis Steyskal, 1954 Notes: BOLD:AAG6869 Tetanocera plumosa Loew, 1847 Trypetoptera canadensis Macquart, 1843 Family Sepsidae Nemopoda nitidula Fallen, 1820 Notes: BOLD:AAG5640 Saltella sphondylii Schrank, 1803 Sepsis punctum Fabricius, 1794 Notes: BOLD:AAG5639 Family Simuliidae Prosimulium arvum Adler and Kim, 1985 Notes: BOLD:AAD4764 Prosimulium mixtum Syme and Davies, 1958 Simulium vittatum Zetterstedt, 1838 Notes: BOLD:AAA4121 Family Sphaeroceridae Apteromyia claviventris Strobl, 1909 Notes: BOLD:AAG7283 Coproica acutangula Zetterstedt, 1847 Coproica ferruginata Stenhammar, 1854 Notes: BOLD:AAN6407 55 56 Telfer A et al. Coproica hirtula Rondani, 1880 Notes: BOLD:ACF7714 Copromyza equina Fallen, 1820 Notes: BOLD:AAJ7412 Gonioneura spinipennis Haliday, 1836 Ischiolepta pusilla Fallen, 1820 Leptocera caenosa Rondani, 1880 Leptocera erythrocera Becker, 1919 Notes: BOLD:AAG7276 Lotophila atra Meigen, 1830 Minilimosina fungicola Spuler, 1925 Minilimosina intercepta Marshall, 1985 Notes: BOLD:AAG7309 Opalimosina mirabilis Collin, 1902 Pullimosina heteroneura Haliday, 1836 Pullimosina longicosta Spuler, 1925 Pullimosina pullula Zetterstedt, 1847 Rachispoda fumipennis-group Spuler 1924 Rachispoda limosa Fallén, 1820 Rachispoda lutosa-group Stenhammar, 1855 Sclerocoelus sordipes Adams 1904 Spelobia bifrons Stenhammar, 1854 Spelobia clunipes Meigen, 1830 Spelobia luteilabris Rondani, 1880 Spelobia ochripes Meigen, 1830 Notes: BOLD:AAG7279 Spelobia semioculata Richards, 1965 Sphaerocera curvipes Latreille, 1805 Telomerina flavipes Meigen, 1830 Notes: BOLD:ACJ1971 Trachyopella nuda Rohacek and Marshall, 1985 Family Stratiomyidae Actina viridis Say, 1824 Notes: BOLD:AAP7640 Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Allognosta fuscitarsis Say, 1823 Allognosta obscuriventris Loew, 1863 Caloparyphus tetraspilus Loew, 1866 Euparyphus stigmaticalis Loew, 1866 Microchrysa polita Linnaeus, 1758 Nemotelus bruesii Melander, 1903 Nemotelus centralis Hanson, 1958 Psellidotus meganticus Curran, 1925 Ptecticus gigliotosi McFadden, 1971 Sargus decorus Say, 1824 Stratiomys norma Wiedemann, 1830 Stratiomys obesa Loew, 1866 Family Syrphidae Allograpta micrura Osten Sacken, 1877 Allograpta obliqua Say, 1823 Notes: BOLD:AAD8276 Brachyopa sedmani Brachypalpus oarus Walker, 1849 Notes: BOLD:AAP8757 Chalcosyrphus libo Walker, 1849 Notes: BOLD:AAG4679 Chalcosyrphus nemorum Fabricius, 1805 Notes: BOLD:AAG6762 Chrysotoxum pubescens Loew, 1864 Dasysyrphus venustus Meigen Notes: BOLD:ACV5348 Epistrophe nitidicollis Meigen, 1822 Eristalis arbustorum Linnaeus, 1758 Eristalis dimidiata Wiedemann, 1830 Eristalis flavipes Walker, 1849 Eristalis stipator Osten Sacken, 1877 57 58 Telfer A et al. Eristalis tenax Linnaeus, 1758 Eristalis transversa Wiedemann, 1830 Eumerus sp. Eupeodes americanus Wiedemann, 1830 Eupeodes volucris Osten Sacken, 1877 Ferdinandea buccata Loew, 1863 Notes: BOLD:AAE0948 Helophilus fasciatus Walker, 1849 Heringia salax Loew, 1866 Lejota aerea Loew, 1872 Notes: BOLD:AAY9807 Mallota posticata Fabricius, 1805 Melanotstoma mellinum Linnaeus 1758 Merodon equestris Fabricius, 1794 Microdon tristis Loew, 1864 Myolepta nigra Loew, 1972 Notes: BOLD:AAV0836 Neoascia distincta Williston, 1887 Notes: BOLD:AAG6766 Ocyptamus fuscipennis Macquart, 1834 Orthonevra nitida Wiedemann, 1830 Paragus haemorrhous Meigen, 1822 Notes: BOLD:AAC2439 Parhelophilus laetus Loew, 1963 Platycheirus hyperboreus Staeger, 1845 Notes: BOLD:ACF4734 Platycheirus obscurus Say, 1824 Notes: BOLD:AAF1237 Platycheirus quadratus Say, 1823 Platycheirus scambus Staeger, 1843 Sericomyia chrysotoxoides Macquart, 1842 Notes: BOLD:ABX5395 Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Sphaerophoria asymmetrica Knutson, 1973 Sphaerophoria bifurcata Knutson, 1973 Sphaerophoria brevipilosa Knutson, 1973 Sphaerophoria contigua Macquart, 1847 Sphaerophoria philanthus Meigen Sphegina keeniana Williston, 1887 Notes: BOLD:ACR0385 Sphegina petiolata Coquillett, 1910 Spilomyia longicornis Loew, 1872 Syritta pipiens Linnaeus, 1758 Syrphus rectus Osten Sacken, 1875 Syrphus ribesii Linnaeus Notes: BOLD:AAA4570 Syrphus torvus Osten Sacken, 1875 Notes: BOLD:AAC6088 Toxomerus geminatus Say, 1923 Notes: BOLD:AAC1312 Toxomerus marginatus Say, 1823 Notes: BOLD:AAA4277 Xanthogramma flavipes Loew, 1863 Notes: BOLD:AAK0114 Xylota quadrimaculata Loew, 1866 Family Tabanidae Chrysops aestuans Wulp, 1867 Chrysops ater Macquart, 1850 Notes: BOLD:ACE5640 Chrysops calvus Pechuman and Teskey, 1967 Chrysops lateralis Wiedemann, 1828 Chrysops striatus Osten Sacken, 1875 Chrysops vittatus Wiedemann, 1821 Hybomitra epistates Osten Sacken, 1878 Hybomitra lasiophthalma Macquart, 1838 Notes: BOLD:AAF0889 59 60 Telfer A et al. Tabanus lineola Fabricius Family Tachinidae Actia interrupta Curran, 1933 Blepharomyia pagana Meigen, 1824 Notes: BOLD:AAV0903 Campylocheta teliosis Reinhard, 1952 Ceracia dentata Coquillett, 1895 Notes: BOLD:ABX6290 Homalactia harringtoni Coquillett, 1902 Notes: BOLD:AAP2717 Leschenaultia exul Townsend, 1892 Notes: BOLD:ACE2864 Lydina americana Townsend, 1892 Notes: BOLD:AAG2432 Oswaldia minor Curran, 1925 Notes: BOLD:ACF1129 Phorocera obscura Fallen, 1810 Notes: BOLD:ABY8575 Siphona hokkaidensis Mesnil, 1957 Notes: BOLD:AAG2172 Siphona intrudens Curran, 1932 Notes: BOLD:AAP2721 Siphona pisinnia O'Hara, 1983 Notes: BOLD:AAZ4865 Family Tephritidae Euaresta bella Loew, 1862 Eurosta solidaginis Fitch, 1855 Eutreta novaeboracensis Fitch, 1855 Rhagoletis suavis Loew, 1862 Urophora cardui Linnaeus, 1758 Xanthomyia platyptera Loew, 1873 Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Family Tipulidae Nephrotoma cornicina Linnaeus, 1758 Tipula (Beringotipula) coloradensis Doane, 1911 Tipula dorsimacula Walker, 1848 Notes: BOLD:AAF8990 Tipula mallochi Alexander Family Xylomyidae Macroceromys terminalis Vasey 1977 Solva pallipes Loew, 1863 Xylomyia americana Weid Xylomyia simillima Steyskal 1947 Xylomyia tenthredinoides Van der Wulp 1867 Family Xylophagidae Xylophagus lugens Loew, 1863 Notes: BOLD:AAJ9649 Xylophagus reflectens Walker, 1848 Notes: BOLD:AAM7333|BOLD:AAP7637 Order Ephemeroptera Family Baetidae Baetis intercalaris McDunnough, 1921 Callibaetis ferrugineus Walsh, 1862 Cloeon dipterum Linnaeus, 1761 Iswaeon anoka Daggy, 1945 Family Caenidae Caenis latipennis Banks, 1907 Family Ephemeridae Hexagenia limbata Serville, 1829 Family Heptageniidae Stenacron interpunctatum Say, 1839 Order Hemiptera Family Anthocoridae Cardiastethus borealis Kelton, 1977 Orius insidiosus Say, 1832 Orius tristicolor White, 1879 61 62 Telfer A et al. Family Aphididae Acyrthosiphon malvae Mosley, 1841 Notes: BOLD:AAF3206 Acyrthosiphon pisum Harris, 1776 Aphis glycines Matsumura, 1917 Notes: BOLD:AAB7938 Aphis middletonii Thomas, 1879 Notes: BOLD:AAB6817 Aphis rubicola Oestlund, 1887 Notes: BOLD:AAF7621 Eriosoma americanum Riley, C.V., 1879 Notes: BOLD:AAD7955 Eucallipterus tiliae Linnaeus, 1758 Notes: BOLD:AAD0131 Lipaphis pseudobrassicae Davis, 1914 Notes: BOLD:AAD9153 Melaphis rhois Fitch, 1866 Notes: BOLD:AAA2079 Rhopalosiphum nymphaeae Linnaeus, 1761 Schizaphis scirpicola Hille Ris Lambers, 1960 Notes: BOLD:AAD1238 Family Belostomatidae Belostoma flumineum Say, 1832 Family Berytidae Neoneides muticus Say, 1832 Family Cercopidae Aphrophora alni Fallén, 1805 Aphrophora cribrata Walker, 1851 Clastoptera obtusa Say, 1825 Lepyronia quadrangularis Say, 1825 Neophilaenus lineatus Linnaeus, 1758 Philaenus spumarius subsp. quadrimaculatus Schrank, 1776 Philaenus spumarius Linnaeus, 1758 Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Family Cicadellidae Agallia quadripunctata Oman 1933 Notes: BOLD:AAG2899 Agallia sp. Agalliopsis ancistra Oman 1970 Agalliopsis sp. Anoscopus flavostriatus Donovan, 1799 Anoscopus flavostrigata Donovan, 1799 Aphrodes sp. Arboridia sp. Athysansus argentarius Metcalf, 1955 Balclutha sp. Ceratagallia sp. Chlorotettix unicolor Fitch 1851 Cicadula melanogaster Provancher 1872 Colladonus clitellarius Say, 1830 Notes: BOLD:ACV9851 Cosmotettix cf. bilineatus Cuerna striata Walker, 1851 Deltocephalus pulicaris Fallén 1806 Notes: BOLD:AAY8918 Dicraneura sp. Dikraneura mali Provancher, 1890 Notes: BOLD:ABA5842 Dikrella cruentata Gillette, 1898 Notes: BOLD:AAV0158 Diplocolenus abdominalis Fabricius 1803 Notes: BOLD:AAG2897 Doratura stylata Boheman, 1847 Notes: BOLD:AAG8821 Draeculacephala constricta Davidson et DeLong, 1943 Draeculacephala sp. 63 64 Telfer A et al. Elymana caduca DeLong 1936 Empoasca coccinea Fitch, 1851 Notes: BOLD:ABA5764 Empoasca decipiens Zachvatkin, 1935 Notes: BOLD:AAY6741 Empoasca fabae Cardoso, 1974 Notes: BOLD:AAG2868|BOLD:AAG2873 Empoasca sp. Erasmoneura nigra Gillette, 1898 Eratoneura certa Beamer, 1932 Notes: BOLD:ABA5787 Eratoneura flexibilis Knull, 1949 Notes: BOLD:AAZ8495|BOLD:AAZ8496 Errastunus ocellaris Fallén, 1806 Notes: BOLD:AAG8839 Erythridula dunni Hepner, 1976 Notes: BOLD:ABA5786 Erythridula scytha Auten & Johnson, 1936 Notes: BOLD:AAN8412 Erythridula tenuispica Beamer, 1930 Notes: BOLD:ABA5830 Erythridula wysongi Ross & DeLong, 1953 Notes: BOLD:AAN8287|BOLD:ABZ1306 Erythroneura aza Robinson, 1924 Notes: BOLD:AAY6747 Erythroneura bakeri Dmitriev & Dietrich, 2007 Notes: BOLD:AAV0161 Erythroneura elegans McAtee, 1920 Notes: BOLD:ABA5798 Erythroneura ontari Robinson, 1924 Notes: BOLD:ABA5810 Erythroneura ontari Robinson, 1924 Notes: BOLD:ABA5810 Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... 65 Checklist of species observed or collected at the rare Charitable Research Reserve in Cambridge, Ontario, Canada. The third of five checklists for Kingdom Animalia, this checklist contains members of Phylum Arthopoda, Class Insecta (Orders Hemiptera, Hymenoptera and Lepidoptera). Class Insecta Order Hemiptera Family Cicadellidae Erythroneura rubrella McAtee, 1920 Notes: BOLD:AAV0164|BOLD:ACC8414 Erythroneura sp. Erythroneura tricincta Fitch, 1851 Notes: BOLD:AAY6738|BOLD:AAY6751 Erythroneura vitifex Fitch, 1856 Notes: BOLD:AAY6742|BOLD:ACQ8506 Erythroneura vitis Harris, 1831 Erythroneura vulnerata Fitch, 1851 Notes: BOLD:AAO8361|BOLD:AAY6752|BOLD:ABA5772|BOLD:ABY9043|BO LD:ABY9046|BOLD:ACQ3943| BOLD:ACV2800|BOLD:ACV2885|BOLD:ACV28 86 Eupteryx atropunctata Goeze, 1778 Notes: BOLD:AAG2869 Eupteryx flavoscuta Gillette, 1898 Notes: BOLD:ABA5805 Fieberiella florii Stål, 1864 Forcipata acclina DeLong & Caldwell, 1936 Forcipata loca DeLong & Caldwell, 1936 Notes: BOLD:ACC8165 66 Telfer A et al. Graphocephala coccinea Forster, 1771 Graphocephala sp. Gyponana sp. Hymetta balteata McAtee, 1919 Notes: BOLD:AAV0157 Jikradia olitoria Say, 1830 Latalus ocellaris De Long & Sleesman, 1929 Macropsis basalis Van Duzee, 1889 Notes: BOLD:ACC9200 Macrosteles quadrilineatus Forbes, 1885 Notes: BOLD:AAA9422 Macrosteles sp. Macrosteles variatus Fallén, 1806 Notes: BOLD:AAV0236 Neokolla hieroglyphica Say, 1830 Notes: BOLD:AAN8418 Oncopsis sobria Walker, 1851 Notes: BOLD:ACI7197 Osbornellus limosus DeLong, 1941 Penthimia americana Fitch, 1851 Scaphoideus major Osborn, 1900 Notes: BOLD:AAY6734 Scaphoideus sp. Scaphytopius sp. Sorhoanus pascuellus Fallén, 1826 Typhlocyba arsinoe McAtee, 1926 Typhlocyba niobe McAtee, 1926 Notes: BOLD:ABA5877 Typhlocyba pomaria McAtee, 1926 Notes: BOLD:AAF5980 Zonocyba hockingensis Knull, 1945 Notes: BOLD:ACV8488 Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Family Cicadidae Tibicen canicularis Harris, 1841 Family Clastopteridae Clastoptera proteus Fitch, 1851 Family Corixidae Palmacorixa buenoi Abbott, 1913 Sehirus cinctus Palisot, 1811 Trichocorixa borealis Sailer, 1948 Trichocorixa sexcincta Champion, 1901 Family Cydnidae Sehirus cinctus cinctus Palisot, 1811 Family Cymidae Cymus angustatus Stål, 1874 Family Delphacidae Delphacodes sp. Javasella pellucida Fabricius, 1794 Kosswigianella lutulenta Van Duzee, 1894 Liburniella ornata Stål, 1862 Megamelus lunatus Beamer, 1955 Pissonotus basalis Van Duzee, 1897 Family Derbidae Cedusa incisa Metcalf, 1923 Family Dictyopharidae Scolops sulcipes Say, 1825 Family Flatidae Metcalfa pruinosa Say, 1830 Family Gelastocoridae Gelastocoris oculatus Fabricius, 1798 Family Gerridae Aquarius remigis Say, 1832 Family Issidae Acanalonia bivittata Say, 1825 67 68 Telfer A et al. Family Lygaeidae Kleidocerys resedae subsp. geminatus Say, 1832 Lygaeus kalmii Stål, 1874 Nysius sp. Family Membracidae Atymna helena Woodruff, 1915 Notes: BOLD:AAY9905 Campylenchia latipes Say, 1824 Ceresa sp. Cyrtolobus sp. Enchenopa binotata Say, 1824 Entylia carinata Forster, 1771 Micrutalis calva Say, 1831 Publilia concava Say, 1824 Telamona decorata Ball, 1903 Family Miridae Adelphocoris lineolatus Goeze, 1778 Amblytylus nasutus Kirschbaum, 1856 Capsus ater Linnaeus, 1758 Ceratocapsus sp. Chlamydatus associatus Uhler, 1872 Notes: BOLD:AAF3365 Chlamydatus sp. Collaria meilleurii Provancher, 1872 Criocoris saliens Reuter, 1876 Deraeocoris sp. Fulvius slateri Wheeler, 1977 Halticus sp. Heterocordylus malinus Slingerland, 1909 Leptopterna dolabrata Linnaeus, 1758 Litomiris sp. Lopidea sp. Lygidea sp. Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Lygocoris pabulinus Linnaeus, 1761 Notes: BOLD:AAB2218 Lygus sp. Nabicula subcoleoptrata Kirby, 1837 Nabis roseipennis Reuter, 1872 Neolygus sp. Orthocephalus sp. Orthops scutellatus Uhler, 1877 Pagasa fusca Stein, 1857 Paraproba capitata Van Duzee, 1912 Phoenicocoris strobicola Knight, 1923 Notes: BOLD:AAH8507 Phytocoris sp. Pithanus maerkelii Herrich-Schaeffer, 1838 Plagiognathus sp. Poecilocapsus lineatus Fabricius, 1798 Prepops sp. Slaterocoris sp. Stenodema trispinosa Reuter, 1904 Stenodema vicinum Provancher, 1872 Stenotus binotatus Fabricius, 1794 Taedia sp. Family Nabidae Hoplistoscelis sordidus Reuter, 1872 Nabis rufusculus Reuter, 1872 Family Notonectidae Notonecta undulata Say, 1832 Family Oxycarenidae Crophius disconotus Say, 1832 Family Pachygronthidae Phlegyas abbreviatus Uhler, 1876 Family Pentatomidae Acrosternum hilare Say, 1832 69 70 Telfer A et al. Banasa calva Say, 1832 Banasa dimidiata Say, 1832 Brochymena quadripustulata Fabricius, 1775 Cosmopepla lintneriana Kirkaldy, 1909 Euschistus tristigmus subsp. luridus Dallas, 1851 Neottiglossa undata Say, 1832 Parabrochymena arborea Say,1825 Picromerus bidens Linnaeus, 1758 Podisus maculiventris Say, 1832 Family Phymatidae Phymata americana Melin, 1930 Piesma cinerum Say, 1832 Family Psyllidae Pachypsylla celtidismamma Fletcher, 1882 Psyllopsis fraxinicola Foerster, 1848 Family Reduviidae Empicornis errabundus Say, 1832 Sinea diadema Fabricius, 1776 Zelus luridus Stål, 1862 Zelus tetracanthus Stål, 1862 Family Rhopalidae Arhyssus sp. Harmostes reflexulus Say, 1832 Stictopleurus punctiventris Dallas, 1852 Family Rhyparochromidae Ligyrocoris diffusus Uhler, 1871 Megalonotus sabulicola Thomson, 1870 Sphragisticus nebulosus Fallén, 1807 Family Saldidae Saldula confluenta Say, 1832 Saldula pallipes Fabricius, 1794 Saldula saltatoria Linnaeus, 1758 Family Thyreocoridae Corimelaena pulicaria Germar, 1839 Galgupha atra Amyot and Serville, 1843 Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Family Tingidae Corythucha marmorata Uhler, 1878 Corythucha sp. Dictyla echii Schrank, 1782 Order Hymenoptera Family Andrenidae Andrena andrenoides Cresson, 1878 Andrena barbilabris Kirby, 1802 Notes: BOLD:AAB4998 Andrena canadensis Dalla Torre, 1896 Andrena carlini Cockerell, 1901 Andrena erythrogaster Ashmead, 1890 Andrena hirticincta Provancher, 1888 Andrena nasonii Robertson, 1895 Andrena obscuripennis Smith, 1853 Andrena rudbeckiae Robertson, 1891 Andrena solidaginis Robertson, 1893 Andrena sp. Andrena thaspii Graenicher, 1903 Andrena wilkella Kirby, 1802 Perdita octomaculata Say, 1824 Pseudopanurgus nebrascensis Crawford, 1903 Family Anthophoridae Anthophora furcata Panzer, 1798 Ceratina calcarata Robertson, 1900 Ceratina dupla Say, 1837 Ceratina sp. Melissodes desponsa Smith, 1854 Family Apidae Apis mellifera Linnaeus, 1758 Bombus bimaculatus Cresson, 1863 Bombus borealis Kirby, 1837 71 72 Telfer A et al. Bombus griseocollis DeGeer, 1773 Bombus impatiens Cresson, 1863 Notes: BOLD:ABZ2516 Bombus perplexus Cresson, 1863 Bombus rufocinctus Cresson, 1863 Bombus vagans Smith, 1854 Ceratina mikmaqi Rehan and Sheffield, 2011 Notes: BOLD:AAA2368 Melissodes boltoniae Robertson, 1905 Melissodes communis Cresson, 1878 Melissodes druriella Kirby, 1802 Melissodes illata Lovell & Cockerell, 1906 Melissodes subillata LaBerge, 1961 Melissodes trinodis Robertson, 1901 Melissodes wheeleri Cockerell, 1906 Nomada articulata Smith, 1854 Nomada bella Cresson, 1863 Notes: BOLD:ABZ2527 Nomada bethunei Cockerell, 1903 Nomada cressonii Robertson, 1893 Nomada luteoloides Robertson, 1895 Nomada pygmaea Cresson, 1863 Notes: BOLD:ABZ6834 Nomada subrutila Lovell & Cockerell, 1905 Notes: BOLD:AAC5044 Triepeolus helianthi Robertson, 1897 Triepeolus nigrihirtus Mitchell, 1962 Triepeolus obliteratus Graenicher, 1911 Triepeolus simplex Robertson, 1903 Xylocopa virginiana Linnaeus, 1771 Family Bethylidae Parasierola sp. Family Braconidae Aphidius ervi Haliday, 1834 Notes: BOLD:AAA4188 Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Ascogaster quadridentata Wesmael, 1835 Asobara rufescens Foerster, 1862 Notes: BOLD:AAU8583 Asobara sp. Cotesia xylina Say, 1836 Notes: BOLD:AAA9386 Diaeretiella rapae McIntosh, 1855 Notes: BOLD:AAG1421 Diolcogaster facetosa Weed, 1888 Notes: BOLD:ABA5941 Ephedrus lacertosus Haliday, 1833 Notes: BOLD:ACW2698 Peristenus sp. Pholetesor ornigis Weed, 1887 Notes: BOLD:AAB0520 Pygostolus falcatus Nees,1834 Spathius elegans Matthews, 1970 Family Chalcididae Conura albifrons Walsh, 1861 Notes: BOLD:AAG8371 Family Chrysididae Cleptes semiauratus Linnaeus, 1761 Family Colletidae Colletes eulophi Robertson, 1891 Colletes hyalinus Provancher, 1888 Colletes mandibularis Smith, 1853 Colletes nudus Robertson, 1898 Colletes simulans Cresson, 1868 Hylaeus affinis Smith, 1853 Hylaeus cf. affinis Hylaeus ellipticus Kirby, 1827 Hylaeus mesillae subsp. cressoni Cockerell, 1907 Hylaeus modestus Say, 1837 Hylaeus sp. 73 74 Telfer A et al. Family Crabronidae Astata unicolor Say, 1824 Cerceris arelate Banks, 1912 Cerceris atramontensis Banks, 1913 Cerceris clypeata Dahlbom, 1844 Crossocerus annulipes Lepeletier et Brullé, 1834 Crossocerus barbipes Dahlbom, 1845 Notes: BOLD:AAG3190 Crossocerus elongatulus van der Linden, 1829 Crossocerus tarsatus subsp. planipes Fox, 1895 Diodontus minutus Fabricius, 1793 Ectemnius cephalotes Olivier, 1792 Ectemnius continuus Fabricius, 1804 Ectemnius lapidarius Panzer, 1804 Ectemnius maculosus Gmelin, 1781 Ectemnius stirpicola Packard, 1866 Gorytes atricornis Packard,1867 Gorytes simillimus Smith,1856 Hoplisoides nebulosus Packard, 1867 Lestica confluenta Say,1837 Lestica producticollis Packard,1866 Lyroda subita Say, 1837 Mimesa pauper Packard, 1867 Oxybelus uniglumis Linnaeus, 1758 Passaloecus cuspidatus Smith, 1856 Notes: BOLD:AAG7762 Passaloecus singularis Dahlbom, 1844 Pemphredon inornata Say, 1824 Pemphredon lethifer Shuckard, 1837 Philanthus bilunatus Cresson, 1865 Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Pison koreense Radoszkowski, 1887 Psen monticola Packard, 1867 Rhopalum coarctatum Scopoli, 1763 Saygorytes phaleratus Say, 1837 Solierella peckhami Ashmead, 1897 Stigmus americanus Packard, 1867 Notes: or fraternus Stigmus fraternus Say, 1824 Tachysphex antennatus Fox, 1894[", 1893"] Tachysphex pompiliformis Panzer, 1803 Trypoxylon attenuatum Smith, 1851 Trypoxylon carinatum Say, 1837 Trypoxylon clavicerum Lepeletier & Serville, 1828 Trypoxylon frigidum Smith, 1856 Notes: BOLD:AAG3193 Trypoxylon johnsoni Fox, 1891 Trypoxylon lactitarse Saussure, 1867 Trypoxylon politum Drury, 1773 Family Diapriidae Belyta validicornis Thomson, 1858 Notes: BOLD:AAU8736 Family Encyrtidae Copidosoma floridanum Ashmead, 1900 Notes: BOLD:AAA7203 Family Eupelmidae Eupelmus vesicularis Retzius, 1783 Family Formicidae Camponotus herculeanus Linnaeus, 1758 Notes: BOLD:AAA2372 Camponotus nearcticus Emery, 1893 Notes: BOLD:AAD4432 Camponotus pennsylvanicus De Geer, 1773 Notes: BOLD:AAA9461 Camponotus sp. 75 76 Telfer A et al. Crematogaster cerasi Fitch, 1855 Crematogaster sp. Formica lasioides Emery, 1893 Notes: BOLD:AAE0406 Formica sp. Lasius alienus Foerster, 1850 Notes: BOLD:AAA9049 Lasius claviger Roger, 1862 Lasius nearcticus Wheeler, 1906 Notes: BOLD:AAD1528 Lasius neoniger Emery, 1893 Notes: BOLD:AAB9126 Lasius sp. Leptothorax ambiguus Emery, 1895 Myrmica sp. Ponera pennsylvanica Buckley, 1866 Notes: BOLD:AAF0443 Prenolepis imparis Say, 1836 Notes: BOLD:AAC1302 Stenamma brevicorne Mayr, 1886 Notes: BOLD:AAH7068 Stigmatomma pallipes Haldeman, 1844 Tapinoma sessile Say, 1836 Notes: BOLD:AAA3893|BOLD:AAA3900 Temnothorax ambiguus Emery, 1895 Notes: BOLD:AAG0685 Family Halictidae Agapostemon radiatus Forster, 1771 Agapostemon sericeus Förster, 1771 Agapostemon virescens Fabricius, 1775 Augochlora aurata Smith, 1853 Augochlora pura Say, 1837 Augochlorella aurata Smith, 1853 Notes: BOLD:AAD6445 Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Dialictus sp. Halictus confusus Smith, 1853 Halictus ligatus Say, 1837 Halictus rubicundus Christ, 1791 Lasioglossum anomalum Robertson, 1892 Lasioglossum atwoodi Gibbs, 2010 Lasioglossum birkmanni Crawford, 1906 Lasioglossum bruneri Crawford, 1902 Lasioglossum comagenense (Knerer & Atwood, 1964) Lasioglossum coriaceum Smith, 1853 Notes: BOLD:AAB7007 Lasioglossum cressonii Robertson, 1890 Lasioglossum dreisbachi Mitchell, 1960 Lasioglossum ephialtum Gibbs, 2010 Lasioglossum fattigi Mitchell, 1960 Lasioglossum foxii Robertson, 1895 Lasioglossum heterognathum Mitchell, 1960 Lasioglossum hitchensi Gibbs, 2012 Lasioglossum imitatum Smith, 1853 Lasioglossum leucozonium Schrank, 1781 Lasioglossum lineatulum Crawford, 1906 Lasioglossum macoupinense Robertson, 1895 Lasioglossum michiganense Mitchell, 1960 Lasioglossum nigroviride Graenicher, 1911 Lasioglossum paradmirandum Knerer & Atwood, 1966 Lasioglossum pectinatum Robertson, 1890 Lasioglossum pectorale Smith, 1853 Lasioglossum perpunctatum Ellis, 1913 Lasioglossum pilosum Smith, 1853 Lasioglossum platyparium Robertson, 1895 Lasioglossum pruinosum Robertson, 1892 Lasioglossum sp. 1 Lasioglossum sp. 2 77 78 Telfer A et al. Lasioglossum subversans Mitchell, 1960 Lasioglossum tegulare Robertson, 1890 Lasioglossum tenax Sandhouse, 1924 Lasioglossum timothyi Gibbs, 2010 Lasioglossum versans Lovell, 1905 Notes: BOLD:ABZ6180 Lasioglossum versatum Robertson, 1902 Lasioglossum vierecki Crawford, 1904 Lasioglossum weemsi Mitchell, 1960 Lasioglossum zonulum Smith, 1848 Lasioglossum zophops Ellis, 1914 Sphecodes atlantis Mitchell, 1956 Sphecodes clematidis Robertson, 1897 Sphecodes confertus Say, 1837 Sphecodes cressonii Robertson, 1903 Sphecodes davisii Robertson, 1897 Sphecodes dichrous Smith, 1853 Sphecodes heraclei Robertson, 1897 Sphecodes levis Lovell & Cockerell, 1907 Sphecodes minor Robertson, 1898 Sphecodes persimilis Lovell & Cockerell, 1907 Sphecodes pycnanthemi Robertson, 1897 Sphecodes ranunculi Robertson, 1897 Notes: BOLD:AAC7655 Sphecodes sp. Sphecodes stygius Robertson, 1893 Sphecodes wheeleri Mitchell, 1956 Family Ichneumonidae Agrothereutes abbreviatus Fabricius, 1794 Notes: BOLD:AAG7687 Agrypon flexorium Thunberg, 1822 Notes: BOLD:AAH7052 Aritranis director Thunberg, 1822 Notes: BOLD:AAG7768 Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Bathyplectes anurus Thomson, 1887 Notes: BOLD:ABA6269 Bathythrix decipiens Gravenhorst, 1829 Notes: BOLD:AAU8495 Campoletis flavicincta Ashmead, 1890 Notes: BOLD:AAZ8146 Cryptus albitarsis Cresson, 1864 Notes: BOLD:AAH1693 Cylloceria melancholica Gravenhorst, 1820 Cymodusa distincta Cresson, 1864 Notes: BOLD:ABZ4364 Diadegma pendulum Walley, 1967 Notes: BOLD:AAZ9563 Dialipsis dissimilis Dasch, 1992 Notes: BOLD:ABA6048 Diplazon laetatorius Fabricius, 1781 Notes: BOLD:AAD4214 Dolichomitus irritator Fabricius, 1775 Notes: BOLD:AAU8680 Dusona minor Provancher, 1879 Notes: BOLD:AAH1652 Enytus apostata Gravenhorst, 1829 Notes: BOLD:AAG5797 Exochus nigripalpis Thomson, 1887 Notes: BOLD:AAG7713 Hyposoter inquinatus Holmgren, 1860 Notes: BOLD:AAU8361 Ichneumon discoensis Fox, 1892 Notes: BOLD:ACE9045 Ischnus inquisitorius Muller, 1776 Notes: BOLD:AAG7737 Iseropus stercorator Fabricius, 1793 Notes: BOLD:AAO2094 79 80 Telfer A et al. Lissonota coracina Gmelin, 1790 Megacara hortulana Gravenhorst, 1829 Notes: BOLD:AAU8687 Mesochorus suomiensis Schwenke, 1999 Notes: BOLD:AAZ1979 Ophion bilineatus Say, 1829 Notes: BOLD:AAG8323 Ophion clave Viereck, 1905 Notes: BOLD:AAG7774 Ophion idoneus Viereck, 1905 Notes: BOLD:AAN8172 Ophion sp. 5 MDS2014 Notes: BOLD:AAI3361 Oresbius taeniatus Townes, 1962 Orthocentrus fulvipes Gravenhorst, 1829 Notes: BOLD:AAM9125 Phobocampe bicingulata Gravenhorst, 1829 Notes: BOLD:AAM7401 Pimpla aequalis Provancher, 1880 Notes: BOLD:AAG7634 Pleolophus basizonus Gravenhorst Podoschistus vittifrons Cresson, 1868 Notes: BOLD:AAL0380 Stenomacrus nemoralis Holmgren, 1858 Notes: BOLD:AAM7494 Tranosema rostrale Brischke, 1880 Notes: BOLD:AAD1926 Zaglyptus varipes Gravenhorst, 1829 Family Leucospidae Leucospis affinis Say, 1824 Family Megachilidae Anthidiellum notatum Latreille, 1809 Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Anthidium manicatum Linnaeus, 1758 Coelioxys rufitarsus Smith, 1854 Heriades variolosa subsp. variolosa Cresson, 1872 Hoplitis pilosifrons Cresson, 1864 Hoplitis producta Cresson, 1864 Hoplitis truncata Cresson, 1878 Megachile brevis Say, 1837 Megachile campanulae Robertson, 1903 Megachile frigide Smith, 1853 Megachile gemula Cresson, 1878 Megachile inermis Provancher, 1888 Megachile latimanus Say, 1823 Megachile lippiae Cockerell, 1900 Megachile mendica Cresson, 1878 Megachile montivaga Cresson, 1878 Megachile perihirta Cockerell, 1898 Megachile pugnata Say, 1837 Megachile relativa Cresson, 1878 Megachile rotundata Fabricius, 1793 Megachile texana Cresson, 1878 Osmia coerulescens Mitchell, T.B. 1962 Osmia conjuncta Cresson, 1864 Osmia lignaria Say, 1837 Notes: BOLD:AAE5495 Osmia proxima Cresson, 1864 Osmia pumila Cresson, 1864 Stelis lateralis Cresson, 1864 Family Mutillidae Myrmosa unicolor Say, 1824 Pseudomethoca frigida Smith 1855 Family Mymaridae Anaphes listronoti Huber, 1997 Gonatocerus morrilli Howard, 1908 Ooctonus silvensis Girault, 1916 Notes: BOLD:AAN7553 81 82 Telfer A et al. Family Platygastridae Platygaster variabilis Fouts, 1924 Notes: BOLD:ABW3242 Synopeas pennsylvanicum Fouts, 1924 Notes: BOLD:ABA6127 Telenomus podisi Ashmead, 1893 Notes: BOLD:AAG7891|BOLD:AAY9192|BOLD:ACU5364 Family Pompilidae Agenioideus cinctellus Spinola, 1808 Agenioideus humilis Cresson, 1867 Anoplius imbellis Banks, 1944 Anoplius nigerrimus van der Vecht, 1973 Anoplius virginiensis Cresson Aporinellus wheeleri Baquaert, 1919 Arachnospila michiganensis subsp. michiganensis (Dreisbach) Auplopus carbonarius Scopoli, 1763 Auplopus mellipes subsp. variitarsatus Dalla Torre, 1897 Auplopus nigrellus Banks, 1912 Caliadurgus fasciatellus subsp. alienatus Smith, 1855 Dipogon sayi subsp. sayi Banks, 1941 Episyron biguttatus subsp. biguttatus Fabricius, 1798 Priocnemis cornica Say, 1836 Priocnemis germana Cresson, 1867 Priocnemis minorata Banks, 1912 Priocnemis notha subsp. notha Cresson, 1867 Priocnemis scitula subsp. relicta Banks, 1912 Family Pteromalidae Mesopolobus bruchophagi Gahan, 1917 Notes: BOLD:ACL4975 Family Sapygidae Sapyga centrata Say, 1836 Notes: BOLD:ACL7820 Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Family Sierolomorphidae Sierolomorpha canadensis Provancher, 1888 Family Siricidae Tremex columba Linnaeus Family Sphecidae Chalybion californicum de Saussure, 1867 Isodontia auripes Fernald, 1906 Isodontia mexicana de Saussure, 1867 Sceliphron caementarium Drury, 1773 Sphex ichneumoneus Linnaeus, 1758 Family Tenthredinidae Ametastegia aperta Norton, 1861 Notes: BOLD:AAI4543 Ametastegia pallipes Spinola Notes: BOLD:AAE5602 Caulocampus acericaulis MacGillivray, 1906 Notes: BOLD:ACJ9109 Dolerus asper Zaddach, 1859 Notes: BOLD:AAG7773 Dolerus nitens Zaddach 1859 Notes: BOLD:ACV5952 Empria maculata Norton, 1861 Notes: BOLD:ACC8799 Empria nordica Ross 1936 Notes: BOLD:ACI4328 Fenusa ulmi Sundevall Notes: BOLD:AAN7643 Halidamia affinis Fallén 1807 Notes: BOLD:AAN7641 Macrophya flavolineata Norton 1860 Notes: BOLD:ABU8852 Metallus lanceolatus Thomson, 1870 Notes: BOLD:AAP1085 83 84 Telfer A et al. Monophadnus pallescens Gmelin 1790 Notes: BOLD:ACK2140 Pachynematus extensicornis Norton Notes: BOLD:AAN8130 Periclista sp. tM8 Notes: BOLD:AAG3550 Priophorus compressicornis Fabricius, 1804 Notes: BOLD:ACI7354 Pristiphora chlorea Norton 1867 Notes: BOLD:ACG2990|BOLD:ACM9731 Taxonus epicera Say 1836 Notes: BOLD:ACC7921 Taxonus pallicoxus Provancher 1885 Notes: BOLD:AAG7788 Taxonus terminalis Say 1824 Notes: BOLD:AAU8702 Tomostethus multicinctus Rohwer Notes: BOLD:ACV5036 Family Trichogrammatidae Trichogramma platneri Nagarkatti, 1975 Notes: BOLD:AAE0242 Family Vespidae Ancistrocerus adiabatus subsp. adiabatus Saussure, 1852 Ancistrocerus albophaleratus de Saussure, 1855 Ancistrocerus antilope subsp. antilope Panzer, 1798 Ancistrocerus campestris Saussure, 1852 Ancistrocerus catskill Saussure, 1853 Ancistrocerus unifasciatus subsp. unifasciatus Saussure, 1852 Dolichovespula arenaria Fabricius Notes: BOLD:ACE9710 Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... 85 Eumenes crucifer Provancher, 1888 Eumenes fraternus Say Euodynerus foraminatus subsp. foraminatus de Saussure, 1853 Euodynerus leucomelas subsp. leucomelas de Saussure, 1856 Monobia quadridens Linnaeus, 1763 Parancistrocerus leionotus Viereck, 1906 Parancistrocerus pedestris de Saussure, 1855 Notes: subsp pedestris? No authorship found Parancistrocerus pensylvanicus de Saussure, 1856 Parancistrocerus pensylvanicus subsp. pensylvanicus de Saussure, 1856 Polistes dominula Christ, 1791 Notes: BOLD:AAB7105 Polistes fuscatus Fabricius, 1793 Symmorphus canadensis Saussure, 1855 Symmorphus cristatus Saussure, 1856 Vespula flavopilosa Jakobson, 1978 Vespula germanica Fabricius, 1793 Notes: BOLD:AAG9055 Vespula maculifrons Buysson Notes: BOLD:AAD5593 Vespula vidua de Saussure, 1854 Notes: BOLD:AAN8137 Vespula vulgaris Linnaeus, 1758 Order Lepidoptera Family Blastobasidae Asaphocrita busckiella Dietz, 1910 Notes: BOLD:AAA8938 Blastobasis glandulella Riley, 1871 Notes: BOLD:AAB1096 Family Bucculatricidae Bucculatrix ainsliella Murtfeldt 1905 Notes: BOLD:AAB4931 Bucculatrix pomifoliella Clemens, 1860 Notes: BOLD:AAD2085 86 Telfer A et al. Family Cosmopterigidae Cosmopterix montisella Chambers, 1875 Notes: BOLD:AAH4285 Teladoma helianthi Busck, 1932 Notes: BOLD:AAE1519 Family Crambidae Acentria ephemerella Denis & Schiffermüller, 1775 Agriphila vulgivagella Clemens, 1860 Anania funebris Ström, 1768 Notes: BOLD:AAB4181 Desmia maculalis Westwood, 1832 Notes: BOLD:ACE8375 Elophila gyralis Hulst, 1886 Elophila icciusalis Walker, 1859 Elophila tinealis Munroe, 1972 Checklist of species observed or collected at the rare Charitable Research Reserve in Cambridge, Ontario, Canada. The fourth of five checklists for Kingdom Animalia, this checklist contains members of Phylum Arthopoda, Class Insecta (Orders Lepidoptera to Trichoptera), and Class Malacostraca. From Phylum Chordata, it contains Classes Actinopterygii, Amphibia, and Aves (Orders Anseriformes to Passeriformes). Kingdom Animalia Phylum Arthropoda Class Insecta Order Lepidoptera Family Crambidae Loxostege sticticalis Linnaeus, 1761 Microcrambus elegans Clemens, 1860 Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Nomophila nearctica Munroe, 1973 Perispasta caeculalis Zell., 1875 Petrophila bifascialis Robinson, 1869 Sitochroa palealis Denis & Schiffermüller, 1775 Udea rubigalis Guenée, 1854 Urola nivalis Drury, 1773 Family Depressariidae Agonopterix arenella Denis & Schiffermüller, 1775 Notes: BOLD:AAC6982 Agonopterix pulvipennella Clemens, 1864 Notes: BOLD:AAA7550 Depressaria depressana Fabricius, 1775 Machimia tentoriferella Clemens, 1860 Family Drepanidae Drepana arcuata Walker Family Elachistidae Perittia herrichiella Herrich-Schaffer, 1855 Family Epermeniidae Epermenia albapunctella Busck, 1908 Notes: BOLD:AAF0142 Family Erebidae Apantesis phalerata Harris, 1841 Cisseps fulvicollis Hübner, 1818 Ctenucha virginica Esper, 1794 Hypena madefactalis Guenée, 1854 Hypena scabra Fabricius, 1798 Hyphantria cunea Drury, 1773 Lymantria dispar subsp. dispar Linnaeus, 1758 Rivula propinqualis Guenée, 1854 Notes: BOLD:AAA4282 Family Gelechiidae Bryotropha hodgesi Rutten & Karsholt, 2004 Notes: BOLD:AAH4276 Chionodes fondella Busck, 1906 Notes: BOLD:ABA4737 87 88 Telfer A et al. Chrysoesthia sexguttella Thunberg, 1794 Notes: BOLD:AAD8505 Dichomeris furia Hodges, 1986 Notes: BOLD:AAI9560 Dichomeris inserrata Walsingham, 1882 Notes: BOLD:AAH4488 Dichomeris leuconotella Busck, 1904 Dichomeris ligulella Hübner, 1818 Notes: BOLD:AAA8109 Dichomeris mercatrix Hodges, 1986 Helcystogramma hystricella Braun, 1921 Notes: BOLD:AAE7016 Metzneria lappella Linnaeus, 1758 Monochroa fragariae Busck, 1919 Scrobipalpa acuminatella Sircom, 1850 Notes: BOLD:AAC1644 Scrobipalpula physaliella Chambers, 1872 Notes: BOLD:ACB8750 Scrobipalpula sacculicola Braun, 1925 Notes: BOLD:ABY8834 Sinoe chambersi Lee, 2012 Notes: BOLD:ACF2217 Xenolechia ontariensis Keifer, 1933 Notes: BOLD:AAC6357 Family Geometridae Alsophila pometaria Harris, 1841 Notes: BOLD:AAB0196 Besma quercivoraria Guenée in Boisduval and Guenée, 1858 Biston betularia Linnaeus, 1758 Campaea perlata Guenée in Boisduval and Guenée, 1858 Coryphista meadii Packard, 1874 Costaconvexa centrostrigaria Wollaston, 1858 Ecliptopera silaceata [Denis and Schiffermüller], 1775 Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Ennomos magnaria Guenée in Boisduval and Guenée, 1858 Epirrhoe alternata Müller, 1764 Euchlaena serrata Drury, 1773 Idaea dimidiata Hufnagel, 1767 Operophtera bruceata Hulst, 1886 Notes: BOLD:AAA2999 Orthonama obstipata Fabricius, 1794 Phigalia titea Cramer, 1780 Notes: BOLD:AAA5234 Plagodis phlogosaria Guenée in Boisduval and Guenée, 1858 Notes: BOLD:AAA3984 Pleuroprucha insulsaria Guenée in Boisduval and Guenée, 1858 Scopula inductata Guenée in Boisduval and Guenée, 1858 Speranza pustularia Guenée in Boisduval and Guenée, 1858 Notes: BOLD:AAA4456 Synchlora frondaria Guenée in Boisduval and Guenée, 1858 Trichodezia albovittata Guenée in Boisduval and Guenée, 1858 Notes: BOLD:AAA6926 Xanthorhoe ferrugata Clerck, 1759 Notes: BOLD:AAA3817 Xanthorhoe lacustrata Guenée in Boisduval and Guenée, 1858 Notes: BOLD:AAA8660 Family Gracillariidae Acrocercops astericola Frey & Boll, 1873 Notes: BOLD:AAD3996 Caloptilia packardella Chambers, 1872 Notes: BOLD:AAD2590 Cameraria saccharella Braun, 1908 Notes: BOLD:AAH4493 Cremastobombycia solidaginis Frey & Boll, 1876 Parornix betulae Stainton, 1854 Notes: BOLD:AAE3418 Parornix crataegifoliella Clemens, 1860 Notes: BOLD:AAF8198 89 90 Telfer A et al. Phyllocnistis ampelopsiella Chambers, 1871 Notes: BOLD:AAI3015 Phyllocnistis vitegenella Clemens, 1859 Notes: BOLD:AAI3014 Phyllonorycter clemensella Chambers, 1871 Notes: BOLD:AAN8981 Phyllonorycter maestingella Müller, 1764 Notes: BOLD:AAL6962 Phyllonorycter ostryaefoliella Clemens, 1859 Notes: BOLD:AAD7999 Phyllonorycter propinquinella Braun, 1908 Notes: BOLD:AAH4497 Phyllonorycter salicifoliella Chambers, 1871 Notes: BOLD:AAD4915 Phyllonorycter trinotella Braun, 1908 Notes: BOLD:AAG1128 Phyllonorycter tritaenianella Chambers, 1871 Notes: BOLD:AAF6577 Family Hesperiidae Anatrytone logan W. H. Edwards, 1863 Ancyloxypha numitor Fabricius, 1793 Atalopedes campestris Boisduval, 1852 Carterocephalus palaemon Pallas, 1771 Epargyreus clarus Cramer, 1775 Erynnis baptisiae W. Forbes, 1936 Erynnis juvenalis Fabricius, 1793 Notes: BOLD:AAC6872 Erynnis lucilius Scudder and Burgess, 1870 Euphyes conspicua W. H. Edwards, 1863 Euphyes dion W. H. Edwards, 1879 Euphyes vestris Boisduval, 1852 Pholisora catullus Fabricius, 1793 Poanes hobomok T. Harris, 1862 Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Poanes massasoit Scudder, 1863 Poanes viator W. H. Edwards, 1865 Polites mystic W. H. Edwards, 1863 Polites origenes Fabricius, 1793 Polites peckius W. Kirby, 1837 Polites themistocles Latreille, 1824 Pompeius verna W. H. Edwards, 1862 Thymelicus lineola Ochsenheimer, 1808 Wallengrenia egeremet Scudder, 1863 Family Lasiocampidae Malacosoma disstria Hubner Notes: BOLD:AAA4130 Tolype velleda Stoll, 1791 Family Lycaenidae Celastrina ladon Cramer, 1780 Celastrina neglecta W. H. Edwards, 1862 Cupido comyntas Godart, 1824 Feniseca tarquinius Fabricius, 1793 Lycaena hyllus Cramer, 1775 Satyirum titus Fabricius, 1793 Satyrium acadicum W. H. Edwards, 1862 Satyrium calanus Hübner, 1809 Satyrium caryaevorus McDunnough, 1942 Satyrium liparops LeConte, 1833 Family Lymantriidae Dasychira basiflava Packard, 1864 Orgyia definita Packard, 1864 Orgyia leucostigma J. E. Smith, 1797 Family Momphidae Mompha terminella Humphreys & Westwood, 1845 Notes: BOLD:AAX4784 Family Nepticulidae Ectoedemia argyropeza Zeller, 1839 Notes: BOLD:AAC1036 91 92 Telfer A et al. Stigmella microtheriella Stainton, 1854 Notes: BOLD:AAI0007 Stigmella rhamnicola Braun, 1916 Notes: BOLD:AAU7678 Family Noctuidae Achatia distincta Hübner, 1813 Notes: BOLD:AAB7392 Agrotis ipsilon Hufnagel, 1766 Agrotis venerabilis Walker, 1857 Allagrapha aerea Hübner, 1803 Amphipoea americana Speyer, 1875 Amphipoea interoceanica Smith, 1899 Amphipyra pyramidoides Guenée, 1852 Notes: BOLD:AAA8525 Anathix ralla Grote and Robinson, 1868 Notes: BOLD:AAC9569 Apamea devastator Brace, 1819 Autographa precationis Guenée, 1852 Caenurgina crassiuscula Haworth, 1809 Catocala cerogama Guenée, 1852 Notes: BOLD:AAB3383 Catocala grynea Cramer, 1779 Cerastis tenebrifera Walker, 1865 Notes: BOLD:AAC1487 Chrysodeixis includens Walker, 1858 Crocigrapha normani Grote, 1874 Notes: BOLD:AAA6924 Cucullia asteroides Guenée, 1852 Cucullia convexipennis Grote and Robinson, 1868 Euplexia benesimilis McDunnough, 1922 Notes: BOLD:AAA4097 Eupsilia devia Grote, 1875 Notes: BOLD:AAD9847 Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Feltia jaculifera Guenée, 1852 Hyppa xylinoides Guenée, 1852 Notes: BOLD:ABY9574 Idia aemula Hübner, 1814 Lacinipolia meditata Grote, 1873 Lacinipolia renigera Stephens, 1829 Leucania commoides Guenée, 1852 Leucania multilinea Walker, 1856 Leucania phragmitidicola Guenée, 1852 Leucania pseudargyria Guenée, 1852 Loscopia velata Walker, 1865 Macrochilo absorptalis Walker, 1859 Melanchra adjuncta Guenée, 1852 Notes: BOLD:ACF4823 Meropleon diversicolor Morrison, 1874 Morrisonia confusa Hübner, 1831 Notes: BOLD:AAA6652 Mythimna unipuncta Haworth, 1809 Noctua pronuba Linnaeus, 1758 Ochropleura implecta Lafontaine, 1998 Oligia modica Guenée, 1852 Orthosia hibisci Guenée, 1852 Notes: BOLD:AAA4128 Orthosia rubescens Walker, 1865 Notes: BOLD:AAC0946 Palthis angulalis Hübner, 1796 Notes: BOLD:AAA3933 Peridroma saucia Hübner, 1808 Phalaenostola metonalis Walker, 1859 Protodeltote albidula Guenée, 1852 Pseudohermonassa bicarnea Guenée, 1852 Renia adspergillus Bosc, 1800 Striacosta albicosta Smith, 1888 Sunira bicolorago Guenée, 1852 Notes: BOLD:AAA4426 93 94 Telfer A et al. Xestia smithii Snellen, 1896 Notes: BOLD:AAA2590 Family Nolidae Nola ovilla Grote, 1875 Notes: BOLD:AAD1810 Family Notodontidae Schizura unicornis J. E. Smith, 1797 Symmerista leucitys Franclemont, 1946 Family Nymphalidae Aglais milberti Godart, 1819 Asterocampa clyton Boisduval and Le Conte, 1835 Boloria Bellona Fabricius, 1775 Boloria selene [Schiffermüller], 1775 Cercyonis pegala Fabricius, 1775 Chlosyne nycteis E. Doubleday, 1847 Coenonympha tullia Müller, 1764 Danaus plexippus Linnaeus, 1758 Euphydryas phaeton Drury, 1773 Euptoieta claudia Cramer, 1775 Junonia coenia Hübner, 1822 Lethe anthedon A. Clark, 1936 Lethe appalachia R. Chermock, 1947 Lethe eurydice Linnaeus, 1763 Libytheana carinenta Cramer, 1777 Limenitis archippus Cramer, 1775 Limenitis arthemis Drury, 1773 Megisto cymela Cramer, 1777 Nymphalis antiopa Linnaeus, 1758 Nymphalis l-album [Schiffermüller], 1775 Phyciodes cocyta Cramer, 1777 Phyciodes tharos Drury, 1773 Polygonia comma T. Harris, 1842 Polygonia interrogationis Fabricius, 1798 Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Polygonia progne Cramer, 1775 Speyeria Cybele Fabricius, 1775 Vanessa atalanta Linnaeus, 1758 Vanessa cardui Linnaeus, 1758 Vanessa virginiensis Drury, 1773 Family Papilionidae Papilio canadensis Rothschild and Jordan, 1906 Papilio cresphontes Cramer, 1777 Papilio glaucus Linnaeus, 1758 Papilio polyxenes Fabricius, 1775 Family Pieridae Colias eurytheme Boisduval, 1852 Colias philodice Godart, 1819 Pieris oleracea T. Harris, 1829 Pieris rapae Linnaeus, 1758 Pyrisitia lisa Boisduval and Le Conte, 1830 Family Plutellidae Plutella porrectella Linnaeus, 1758 Notes: BOLD:ACG9804 Plutella xylostella Linnaeus, 1758 Notes: BOLD:AAA1513 Family Psychidae Psyche casta Pallas, 1767 Notes: BOLD:ACL8669 Family Pterophoridae Gillmeria pallidactyla Haworth, 1811 Hellinsia homodactylus Walker, 1864 Hellinsia pectodactylus Staudinger, 1859 Family Sphingidae Deidamia inscriptum Harris, 1839 Notes: BOLD:AAB0001 Family Tischeriidae Coptotriche badiiella Chambers, 1875 Notes: BOLD:ACU4456 95 96 Telfer A et al. Family Tortricidae Acleris chalybeana Fernald, 1882 Notes: BOLD:AAA7667 Acleris cornana McDunnough, 1933 Ancylis muricana Walsingham, 1879 Notes: BOLD:AAU7760 Argyrotaenia mariana Fernald, 1882 Notes: BOLD:AAA4119 Choristoneura rosaceana Harris, 1841 Notes: BOLD:AAA1517 Cochylis hoffmanana Kearfott, 1907 Notes: BOLD:AAB3571 Cochylis temerana Busck, 1907 Notes: BOLD:AAB7534 Endothenia hebesana Walker, 1863 Epinotia medioviridana Kearfott, 1908 Eucosma similana Clemens, 1860 Grapholita prunivora Walsh, 1868 Notes: BOLD:AAG0330 Olethreutes atrodentana Fernald, 1882 Olethreutes fasciatana Clemens, 1860 Olethreutes permundana Clemens, 1860 Pandemis lamprosana Robinson, 1869 Phaneta ochrocephala Walsingham, 1895 Phaneta parmatana Clemens, 1860 Phaneta tomonana Kearfott, 1907 Platynota idaeusalis Walker, 1859 Notes: BOLD:ABY7901 Pristerognatha fuligana Denis & Schiffermüller, 1775 Notes: BOLD:AAC7661 Proteoteras aesculana Riley, 1881 Notes: BOLD:AAA6740 Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Order Mantodea Family Mantidae Mantis religiosa subsp. religiosa Linnaeus, 1758 Order Mecoptera Family Bittacidae Bittacus strigosus Hagen, 1861 Family Panorpidae Panorpa galerita Byers, 1962 Panorpa latipennis Hine, 1901 Panorpa subfurcata Westwood, 1846 Order Neuroptera Family Chrysopidae Chrysopa oculata Say, 1839 Family Hemerobiidae Hemerobius humulinus Linnaeus, 1758 Notes: BOLD:AAG0892|BOLD:AAN7492 Hemerobius stigma Stephens, 1836 Notes: BOLD:AAG0891 Micromus posticus Walker, 1853 Notes: BOLD:AAG0906 Order Odonata Family Aeshnidae Aeshna umbrosa Walker, 1908 Anax junius Drury, 1773 Family Calopterygidae Hetaerina americana Fabricius, 1798 Family Coenagrionidae Argia moesta Hagen, 1861 Enallagma antennatum Say, 1839 Enallagma civile Hagen, 1861 Enallagma ebrium Hagen, 1861 Enallagma exsulans Hagen, 1861 Enallagma geminatum Kellicott, 1895 Enallagma signatum Hagen, 1861 Ischnura kellicotti Williamson, 1898 97 98 Telfer A et al. Ischnura posita Hagen, 1861 Ishnura verticalis Say, 1839 Nehalennia irene Hagen, 1861 Family Lestidae Lestes disjunctus Selys, 1862 Lestes rectangularis Say, 1839 Family Libellulidae Erythemis simplicicollis Say, 1839 Libellula luctuosa Burmeister, 1839 Libellula pulchella Drury, 1773 Libellula quadrimaculata Linnaeus, 1758 Pachydiplax longipennis Burmeister, 1839 Plathemis lydia Drury, 1773 Sympetrum internum Montgomery, 1943 Sympetrum obtrusum Hagen, 1867 Sympetrum semicinctum Say, 1839 Tramea lacerat Hagen, 1861 Order Orthoptera Family Acrididae Cholealtis conspera Harris, 1841 Chorthippus curtipennis Harris, 1835 Chortophaga viridifasciata De Geer, 1773 Dissosteira carolina Linnaeus, 1758 Dissosteria carolina Linnaeus, 1758 Melanoplus bivatattus Say, 1825 Melanoplus sanguinipes subsp. sanguinipes Fabricius, 1798 Melanoplus sp. Family Gryllidae Allonemobius fasciatus De Geer, 1773 Gryllus veletis Alexander and Bigelow, 1960 Oecanthus nigricornis F. Walker, 1869 Oecanthus quadripunctatus Beutenmuller, 1894 Family Tetrigidae Tetrix arenonsum subsp. angusta Hancock, 1896 Tetrix subulata Linnaeus, 1761 Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Family Tetrigoniidae Conocephalus brevipennis Scudder, 1863 Scudderia sp. Order Phasmatodea Family Heteronemiidae Diapheromera femorata Say, 1824 Order Plecoptera Family Chloroperlidae Sweltsa onkos Ricker, 1935 Order Psocoptera Family Amphipsocidae Polypsocus corruptus Hagen, 1861 Family Caeciliidae Caecillius sp. Family Caeciliusidae Valenzuela flavidus Stephens, 1836 Notes: BOLD:AAH3228|BOLD:AAN8447 Family Ectopsocidae Ectopsocus meridionalis Ribaga, 1904 Family Mesopsocidae Mesopsocus unipunctatus Müller, 1764 Family Peripsocidae Peripsocus subfasciatus Rambur, 1842 Family Psocidae Blaste opposita Banks, 1907 Metylophorus novaescotiae Walker, 1853 Family Stenopsocidae Graphopsocus cruciatus Linnaeus, 1768 Notes: BOLD:ACA2933|BOLD:ACB0984 Order Thysanoptera Family Aeolothripidae Aeolothrips ericae Bagnall, 1920 Notes: BOLD:ABA2981 99 100 Telfer A et al. Family Phlaeothripidae Haplothrips verbasci Osborn, 1897 Notes: BOLD:AAI6861 Family Thripidae Chirothrips manicatus Haliday, 1836 Odontothrips biuncus John, 1921 Taeniothrips inconsequens Uzel, 1895 Notes: BOLD:ACC0651 Order Trichoptera Family Helicopsychidae Helicopsyche borealis Hagen, 1861 Family Hydropsychidae Ceratopsyche morosa Hagen, 1861 Notes: BOLD:AAA3679 Cheumatopsyche campyla Ross, 1938 Notes: BOLD:AAA3892|BOLD:ACE5263 Hydropsyche phalerata Hagen, 1861 Notes: BOLD:AAC3243 Family Hydroptilidae Agraylea multipunctata Curtis, 1834 Hydroptila armata Ross, 1938 Hydroptila perdita Morton, 1905 Notes: BOLD:AAE5187 Hydroptila spatulata Morton, 1905 Notes: BOLD:AAD0137 Orthotrichia cristata Morton, 1905 Family Leptoceridae Oecetis avara Banks, 1895 Oecetis cinerascens Hagen, 1861 Oecetis inconspicua Walker, 1852 Notes: BOLD:AAA1532 Oecetis nocturna Ross, 1966 Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Family Limnephilidae Ironoquia punctatissima Walker, 1852 Pycnopsyche antica Walker, 1852 Family Phryganeidae Banksiola crotchi Banks, 1944 Family Polycentropodidae Plectrocnemia cinerea Hagen, 1861 Notes: BOLD:AAA3441|BOLD:ACL7631 Class Malacostraca Order Amphipoda Family Hyalellidae Hyalella azteca Saussure, 1858 Order Decapoda Family Cambaridae Orconectes propinquus Girard, 1852 Order Isopoda Family Trachelipodidae Trachelipus rathkii Brandt, 1833 Notes: BOLD:AAH4102 Family Trichoniscidae Hyloniscus riparius Koch, 1838 Notes: BOLD:AAV6495 Trichoniscus pusillus Brandt, 1833 Notes: BOLD:AAN7523 Phylum Chordata Class Actinopterygii Order Cypriniformes Family Catostomidae Catostomus catostomus Forster, 1773 Catostomus commersonii Lacepède, 1803 Hypentelium nigricans Lesueur, 1817 Moxostoma erythrurum Rafinesque, 1818 Moxostoma valenciennesi Jordan, 1885 101 102 Telfer A et al. Family Cyprinidae Cyprinella spiloptera Cope, 1867 Cyprinus Carpio Linnaeus, 1758 Luxilus chrysocephalus Rafinesque, 1820 Luxilus cornutus Mitchill, 1817 Nocomis biguttatus Kirtland, 1840 Nocomis micropogon Cope, 1865 Notropis heterodon Cope, 1865 Notropis photogenis Cope, 1865 Pimephales notatus Rafinesque, 1820 Rhinichthys atratulus Hermann, 1804 Semotilus atromaculatus Mitchill, 1818 Order Esociformes Family Esocidae Esox lucius Linnaeus, 1758 Order Gasterosteiformes Family Gasterosteidae Culaea inconstans Kirtland, 1840 Order Perciformes Family Centrarchidae Ambloplites rupestris Rafinesque, 1817 Lepomis gibbosus Linnaeus, 1758 Micropterus dolomieu Lacepède, 1802 Micropterus salmoides Lacepède, 1802 Pomoxis nigromaculatus Lesueur in Cuvier and Valenciennes, 1829 Family Percidae Etheostoma blennioides Rafinesque, 1819 Etheostoma exile Girard, 1859 Etheostoma nigrum Rafinesque, 1820 Perca flavescens Mitchill, 1814 Percina maculata Girard, 1859 Order Salmoniformes Family Salmonidae Salvelinus fontinalis Mitchill, 1814 Order Siluriformes Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... 103 Family Ictaluridae Ameiurus nebulosus Lesueur, 1819 Noturus flavus Rafinesque, 1818 Class Amphibia Order Anura Family Bufonidae Anaxyrus americanus Holbrook, 1836 Family Hylidae Hyla versicolor LeConte, 1825 Pseudacris crucifer Wied-Neuwied, 1838 Pseudacris triseriata Wied-Neuwied, 1838 Family Ranidae Lithobates catesbeiana Shaw, 1802 Lithobates clamitans Latreille in Sonnini de Manoncourt and Latreille, 1801 Lithobates pipiens Schreber, 1782 Lithobates sylvatica LeConte, 1825 Order Caudata Family Ambystomidae Ambystoma jeffersonianum x laterale var. hybrid species: jefferson and blue-spotted Green, 1827 and Hallowell, 1856 Ambystoma laterale Hallowell, 1856 Ambystoma maculatum Shaw, 1802 Family Plethodontidae Hemidactylium scutatum Temminck and Schlegel, 1838 Plethodon cinereus Green, 1818 Class Aves Order Anseriformes Family Anatidae Aix sponsa Linnaeus, 1758 Anas acuta Linnaeus, 1758 Anas americana Gmelin, 1789 Anas clypeata Linnaeus, 1758 Anas crecca Linnaeus, 1758 Anas discors Linnaeus, 1766 Anas platyrhynchos Linnaeus, 1758 104 Telfer A et al. Anas rubripes Brewster, 1902 Anas strepera Linnaeus, 1758 Aythya affinis Eyton, 1838 Aythya americana Eyton, 1838 Aythya collaris Donovan, 1809 Aythya marila Linnaeus, 1761 Aythya valisineria A. Wilson, 1814 Branta bernicla Linnaeus, 1758 Branta canadensis Linnaeus, 1758 Branta hutchinsii Richardson, 1832 Bucephala albeola Linnaeus, 1758 Bucephala clangula Linnaeus, 1758 Chen caerulescens Linnaeus, 1758 Clangula hyemalis Linnaeus, 1758 Cygnus buccinator Richardson, 1831 Cygnus columbianus Ord, 1815 Cygnus olor Gmelin, 1789 Lophodytes cucullatus Linnaeus, 1758 Melanerpes erythrocephalus Linnaeus, 1758 Mergus merganser Linnaeus, 1758 Mergus serrator Linnaeus, 1758 Oxyura jamaicensis Gmelin, 1789 Order Apodiformes Family Apodidae Chaetura pelagica Linnaeus, 1758 Family Trochilidae Archilochus colubris Linnaeus, 1758 Order Caprimulgiformes Family Caprimulgidae Caprimulgus vociferus A. Wilson, 1812 Chordeiles minor J. R. Forster, 1771 Order Charadriiformes Family Charadriidae Charadrius semipalmatus Bonaparte, 1825 Charadrius vociferus Linnaeus, 1758 Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Family Laridae Chroicocephalus philadelphia Ord, 1815 Larus delawarensis Ord, 1815 Larus fuscus Linnaeus, 1758 Larus glaucoides B. Meyer, 1822 Larus hyperboreus Gunnerus, 1767 Larus marinus Linnaeus, 1758 Larus smithsonianus Coues, 1862 Larus thayeri W. S. Brooks, 1915 Mniotilta varia Linnaeus, 1766 Sterna hirundo Linnaeus, 1758 Family Scolopacidae Actitis macularius Linnaeus, 1766 Arenaria interpres Linnaeus, 1758 Bartramia longicauda Bechstein, 1812 Calidris alpina Linnaeus, 1758 Calidris bairdii Coues, 1861 Calidris melanotos Vieillot, 1819 Calidris minutilla Vieillot, 1819 Calidris pusilla Linnaeus, 1766 Gallinago delicata Ord, 1825 Scolopax minor Gmelin, 1789 Tringa flavipes Gmelin, 1789 Tringa melanoleuca Gmelin, 1789 Tringa solitaria A. Wilson, 1813 Family Sternidae Hydroprogne caspia Pallas, 1770 Order Ciconiiformes Family Ardeidae Ardea alba Linnaeus, 1758 Ardea herodias Linnaeus, 1758 Botaurus lentiginosus Rackett, 1813 Butorides virescens Linnaeus, 1758 Chlidonias niger Linnaeus, 1758 Egretta caerulea Linnaeus, 1758 105 106 Telfer A et al. Order Columbiformes Family Columbidae Columba livia Gmelin, 1789 Zenaida macroura (Linnaeus, 1758) Order Coraciiformes Family Alcedinidae Megaceryle alcyon Linnaeus, 1758 Order Cuculiformes Family Cuculidae Coccyzus americanus Linnaeus, 1758 Coccyzus erythropthalmus A. Wilson, 1811 Order Falconiformes Family Accipitridae Accipiter cooperii Bonaparte, 1828 Accipiter gentilis Linnaeus, 1758 Accipiter striatus Vieillot, 1808 Agelaius phoeniceus Linnaeus, 1766 Aquila chrysaetos Linnaeus, 1758 Buteo jamaicensis Gmelin, 1788 Buteo lagopus Pontoppidan, 1763 Buteo lineatus Gmelin, 1788 Buteo platypterus Vieillot, 1823 Circus cyaneus Linnaeus, 1766 Haliaeetus leucocephalus Linnaeus, 1766 Family Cathartidae Cathartes aura Linnaeus, 1758 Family Falconidae Falco columbarius Linnaeus, 1758 Falco peregrinus Tunstall, 1771 Falco sparverius Linnaeus, 1758 Family Pandionidae Pandion haliaetus Linnaeus, 1758 Order Galliformes Family Odontophoridae Colinus virginianus Linnaeus, 1758 Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Family Phasianidae Bonasa umbellus Linnaeus, 1766 Meleagris gallopavo Linnaeus, 1758 Phasianus colchicus Linnaeus, 1758 Order Gaviiformes Family Gaviidae Gavia immer Brunnich, 1764 Order Gruiformes Family Gruidae Grus canadensis Linnaeus, 1758 Family Rallidae Fulica americana Gmelin, 1789 Porzana carolina Linnaeus, 1758 Rallus limicola Vieillot, 1819 Order Passeriformes Family Alaudidae Eremophilia alpestris (Linnaeus, 1758) Family Bombycillidae Bombycilla cedrorum Vieillot, 1808 Family Calcariidae Calcarius lapponicus Linnaeus, 1758 Family Cardinalidae Cardinalis cardinalis Linnaeus, 1758 Passerina cyanea Linnaeus, 1766 Pheucticus ludovicianus Linnaeus, 1766 Plectrophenax nivalis Linnaeus, 1758 Family Certhiidae Certhia americana Bonaparte, 1838 Family Corvidae Corvus brachyrhynchos C. L. Brehm, 1822 Corvus corax Linnaeus, 1758 Cyanocitta cristata Linnaeus, 1758 Family Emberizidae Ammodramus leconteii Audubon, 1844 Ammodramus nelsoni Allen, 1875 107 108 Telfer A et al. Ammodramus savannarum Gmelin, 1789 Junco hyemalis Linnaeus, 1758 Melospiza georgiana Latham, 1790 Melospiza lincolnii Audubon, 1834 Melospiza melodia A. Wilson, 1810 Passerculus sandwichensis Gmelin, 1789 Passerella iliaca Merrem, 1786 Pipilo erythrophthalmus Linnaeus, 1758 Pipilo maculatus Swainson, 1827 Pooecetes gramineus Gmelin, 1789 Spizella arborea A. Wilson, 1810 Spizella pallida Swainson, 1832 Spizella passerina Bechstein, 1798 Spizella pusilla A. Wilson, 1810 Zonotrichia albicollis Gmelin, 1789 Zonotrichia leucophrys J. R. Forster, 1772 Family Fringillidae Carduelis flammea Linnaeus, 1758 Carduelis pinus A. Wilson, 1810 Carduelis tristis Linnaeus, 1758 Carpodacus mexicanus Statius Muller, 1776 Carpodacus purpureus Gmelin, 1789 Coccothraustes vespertinus W. Cooper, 1825 Loxia curvirostra Linnaeus, 1758 Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... 109 Checklist of species observed or collected at the rare Charitable Research Reserve in Cambridge, Ontario, Canada. The fifth of five checklists for Kingdom Animalia, this checklist contains records from Phylum Chordata, Class Aves (Orders Passeriformes to Strigiformes), Class Mammalia, and Class Reptilia, as well as Phylum Gastropoda. Phylum Chordata Class Aves Order Passeriformes Family Fringillidae Loxia leucoptera Gmelin 1789 Family Hirundinidae Hirundo rustica Linnaeus, 1758 Petrochelidon pyrrhonota (Vieillot, 1817) Riparia riparia Linnaeus, 1758 Stelgidopteryx serripennis Audubon, 1838 Tachycineta bicolor Vieillot, 1808 Family Icteridae Dolichonyx oryzivorus Linnaeus, 1758 Euphagus carolinus Statius Muller, 1776 Euphagus cyanocephalus Wagler, 1829 Icterus galbula Linnaeus, 1758 Icterus spurius Linnaeus, 1766 Molothrus ater Boddaert, 1783 Quiscalus quiscula Linnaeus, 1758 Sturnella magna Linnaeus, 1758 Xanthocephalus xanthocephalus Bonaparte, 1826 Family Laniidae Lanius excubitor Linnaeus, 1758 110 Telfer A et al. Family Mimidae Dumetella carolinensis Linnaeus, 1766 Mimus polyglottos Linnaeus, 1758 Toxostoma rufum Linnaeus, 1758 Family Motacillidae Anthus rubescens Tunstall, 1771 Family Paridae Poecile atricapillus Linnaeus, 1766 Family Parulidae Cardellina canadensis Linnaeus, 1766 Cardellina pusilla A. Wilson, 1811 Geothlypis philadelphia A. Wilson, 1810 Geothlypis trichas Linnaeus, 1766 Leiothlypis celata Say, 1822 Leiothlypis peregrina A. Wilson, 1811 Leiothlypis ruficapilla A. Wilson, 1811 Oporornis agilis A. Wilson, 1812 Setophaga americana Linnaeus, 1758 Setophaga caerulescens J. F. Gmelin, 1789 Setophaga castanea A. Wilson, 1810 Setophaga citrina Boddaert, 1783 Setophaga coronata Linnaeus, 1766 Setophaga discolor Vieillot, 1809 Setophaga fusca Statius Müller, 1776 Setophaga magnolia A. Wilson, 1811 Setophaga palmarum J. F. Gmelin, 1789 Setophaga pensylvanica Linnaeus, 1766 Setophaga petechia Linnaeus, 1766 Setophaga pinus Linnaeus, 1766 Setophaga ruticilla Linnaeus, 1758 Setophaga striata J. R. Forster, 1772 Setophaga tigrina J. F. Gmelin, 1789 Setophaga virens J. F. Gmelin, 1789 Vermivora chrysoptera Linnaeus, 1766 Vermivora cyanoptera Olson & Reveal, 2009 Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Family Passeridae Passer domesticus Linnaeus, 1758 Family Peucedramidae Nycticorax nycticorax Linnaeus, 1758 Parkesia noveboracensis J. F. Gmelin, 1789 Picoides arcticus Swainson, 1832 Seiurus aurocapilla Linnaeus, 1766 Family Regulidae Regulus calendula (Linnaeus, 1766) Regulus satrapa Lichtenstein 1823 Family Sittidae Sitta canadensis Linnaeus, 1766 Sitta carolinensis Latham, 1790 Family Sturnidae Sturnus vulgaris Linnaeus, 1758 Family Sylviidae Polioptila caerulea Linnaeus, 1766 Family Thraupidae Piranga olivacea Gmelin, 1789 Family Troglodytidae Cistothorus palustris A. Wilson, 1810 Thryothorus ludovicianus (Latham, 1790) Troglodytes aedon Vieillot, 1809 Troglodytes hiemalis Vieillot, 1819 Family Turdidae Catharus fuscescens (Stephens, 1817) Catharus guttatus Pallas, 1811 Catharus minimus Lafresnaye, 1848 Catharus ustulatus Nuttall, 1840 Hylocichla mustelina Gmelin, 1789 Sialia sialis Linnaeus, 1758 Turdus migratorius Linnaeus, 1766 Family Tyrannidae Contopus cooperi Nuttall, 1831 Contopus virens Linnaeus, 1766 111 112 Telfer A et al. Empidonax alnorum Brewster, 1895 Empidonax flaviventris W. M. Baird & S. F. Baird, 1843 Empidonax Minimus W. M. Baird & S. F. Baird, 1843 Empidonax traillii Audubon, 1828 Myiarchus crinitus Linnaeus, 1758 Sayornis phoebe Latham, 1790 Tyrannus tyrannus Linnaeus, 1758 Family Vireonidae Vireo flavifrons Vieillot, 1808 Vireo gilvus Vieillot, 1808 Vireo griseus Boddaert, 1783 Vireo olivaceus Linnaeus, 1766 Vireo philadelphicus Cassin, 1851 Vireo solitarius A. Wilson, 1810 Order Pelecaniformes Family Phalacrocoracidae Phalacrocorax auritus Lesson, 1831 Order Piciformes Family Picidae Colaptes auratus Linnaeus, 1758 Dryocopus Pileatus Linnaeus, 1758 Melanerpes carolinus Linnaeus, 1758 Picoides pubescens Linnaeus, 1766 Picoides villosus Linnaeus, 1766 Sphyrapicus varius Linnaeus, 1766 Order Podicipediformes Family Podicipedidae Podiceps auritus Linnaeus, 1758 Podiceps grisegena Boddaert, 1783 Podilymbus podiceps Linnaeus, 1758 Order Strigiformes Family Strigidae Aegolius acadicus Gmelin, 1788 Asio flammeus Pontoppidan, 1763 Bubo virginianus Gmelin, 1788 Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Megascops asio Linnaeus, 1758 Strix varia Barton, 1799 Class Mammalia Order Artiodactyla Family Cervidae Odocoileus virginianus Zimmermann, 1780 Order Carnivora Family Canidae Canis latrans Say, 1823 Vulpes vulpes Linnaeus, 1758 Family Felidae Lynx rufus Schreber, 1777 Family Mephitidae Mephitis mephitis Schreber, 1776 Family Mustelidae Lontra canadensis Schreber, 1777 Mustela erminea Linnaeus, 1758 Neovison vison Schreber, 1777 Family Procyonidae Procyon lotor Linnaeus, 1758 Order Chiroptera Family Vespertilionidae Eptesicus fuscus Palisot de Beauvois, 1796 Lasionycteris noctivagans LeConte, 1831 Lasiurus borealis Müller, 1776 Lasiurus cinereus Palisot de Beauvois, 1796 Myotis lucifugus LeConte, 1831 Myotis septentrionalis Trouessart, 1897 Order Didelphimorphia Family Didelphidae Didelphis virginiana Kerr, 1792 Order Lagomorpha Family Leporidae Lepus americanus Erxleben, 1777 Lepus europaeus Pallas, 1778 113 114 Telfer A et al. Sylvilagus floridanus J. A. Allen, 1890 Order Rodentia Family Castoridae Castor canadensis Kuhl, 1820 Family Cricetidae Microtus pennsylvanicus Ord, 1815 Myodes gapperi Vigors, 1830 Ondatra zibethicus Linnaeus, 1766 Peromyscus leucopus Rafinesque, 1818 Peromyscus maniculatus Wagner, 1845 Family Dipodidae Zapus hudsonius Zimmermann, 1780 Family Erethizontidae Erethizon dorsatus Linnaeus, 1758 Family Muridae Mus musculus Linnaeus, 1758 Rattus norvegicus Berkenhout, 1769 Family Sciuridae Glaucomys sabrinus Shaw, 1801 Marmota monax Linnaeus, 1758 Sciurus carolinensis Gmelin, 1788 Tamias striatus Linnaeus, 1758 Tamiasciurus hudsonicus Erxleben, 1777 Order Soricomorpha Family Soricidae Blarina brevicauda Say, 1823 Sorex cinereus Kerr, 1792 Sorex fumeus G. M. Miller, 1895 Family Talpidae Condylura cristata Linnaeus, 1758 Class Reptilia Order Squamata Family Colubridae Lampropeltis triangulum subsp. triangulum (Lacépède, 1789) Nerodia sipedon Linnaeus, 1758 Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Opheodrys vernalis Harlan, 1827 Regina septemvittata Say, 1825 Storeria dekayi Holbrook, 1839 Storeria occipitomaculata Storer, 1839 Thamnophis sauritus Linnaeus, 1766 Thamnophis sirtalis subsp. sirtalis (Linnaeus, 1758) Order Testudines Family Chelydridae Chelydra serpentina Linnaeus, 1758 Family Emydidae Chrysemys picta Schneider, 1783 Phylum Mollusca Class Gastropoda Family Discidae Anguispira alternata Say, 1816 Family Pristilomatidae Hawaiia minuscula A. Binney, 1841 Family Succineidae Novisuccinea ovalis Say, 1817 Family Vertiginidae Vertigo bollesiana E. S. Morse, 1865 Order Basommatophora Family Planorbidae Gyraulus circumstriatus Tryon, 1866 Order Hygrophila Family Physidae Physa gyrina Say, 1821 Order Pulmonata Family Zonitidae Paravitrea multidentata A. Binney, 1840 Order Stylommatophora Family Agriolimacidae Deroceras reticulatum Muller, 1774 Family Arionidae Arion fuscus (O. F. Müller, 1774) 115 116 Telfer A et al. Arion subfuscus Draparnaud, 1805 Family Gastrodontidae Oxychilus allarius (J. S. Miller, 1822) Zonitoides arboreus Say, 1816 Notes: BOLD:AAN3419 Family Helicidae Cepaea nemoralis Linnaeus, 1758 Cochlicopa lubrica (Muller, 1774) Family Helicodiscidae Helicodiscus parallelus Say, 1817 Family Hygromiidae Trochulus hispidus (Linnaeus, 1758) Notes: BOLD:ACI9420 Family Succineidae Succinea putris Linnaeus, 1758 Family Vitrinidae Vitrina angelicae Beck, 1837 Checklist of species observed or collected at the rare Charitable Research Reserve in Cambridge, Ontario, Canada. This checklist contains members of Kingdom Amoebozoa, Phylum Mycetozoa. Class Myxogastria Order Liceales Family Tubiferaceae Lycogala epidendrum Linnaeus, 1829 Notes: / flavofuscum Order Liceida Family Reticulariaceae Tubifera ferruginosa (Batsch) J.F. Gmel. Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... 117 Order Stemonitida Family Stemonitidae Stemonitis axifera Bull, 1791 Checklist of species observed or collected at the rare Charitable Research Reserve in Cambridge, Ontario, Canada. This checklist contains records from Kingdom Fungi, including Phyla Ascomycota, Basidiomycota, and Zygomycota. Phylum Ascomycota Dictyocatenulata alba Finley & E. F. Morris 1967 Phylum Ascomycota Class Arthoniomycetes Order Arthoniales Family Arthoniaceae Arthonia caesia Koerber, 1861 Arthonia caudata Willey, 1892 Arthonia radiata Vetensk-Akad, 1808 Class Dothideomycetes Order Pleosporales Family Venturiaceae Apiosporina morbosa Arx, 1954 Order Polyporales Family Fomitopsidaceae Ischnoderma resinosum Schrader, 1794 Piptoporus betulinus Bulliard, 1780 Postia caesia Schrad, 1881 Notes: (Oligoporus caesius) Postia fragilis Jülich, 1982 Family Ganodermataceae Ganoderma applanatum Persoon, 1799 Ganoderma lucidum Curtis 1791 Notes: or Ganoderma tsugae 118 Telfer A et al. Family Meripilaceae Gloeoporus dichrous Fries, 1815 Grifola frondosa Dickson, 1785 Family Meruliaceae Bjerkandera adusta Willdenow, 1787 Climacodon septentrionale Fries, 1821 Phlebia radiata Fries, 1821 Porotheleum fimbriatum Persoon, 1818 Family Phanerochaetaceae Steccherinum ochraceum Persoon, 1792 Family Polyporaceae Cerrena unicolor Bulliard, 1785 Favolus alveolaris De Candolle 1815 Laetiporus sulphureus Bulliard, 1780 Lenzites betulina Linnaeus, 1753 Polyporus badius Persoon, 1801 Polyporus brumalis Persoon, 1794 Trametes elegans Sprengel, 1820 Trichaptum abietinum Dickson, 1972 Notes: (?) Tyromyces chioneus Fries, 1815 Notes: (Tyromyces albellus, Polyorus albellus Family Steccherinaceae Irpex lacteus Fries, 1818 Class Eurotiomycetes Order Mycocaliciales Family Mycocaliciaceae Mycocalicium subtile Persoon, 1925 Phaeocalicium curtisii Tuckerman, 1975 Order Verrucariales Family Verrucariaceae Verrucaria calkinsiana Servít, 1950 Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Class Lecanoromycetes Order Baeomycetales Family Trapeliaceae Trapelia involuta (Taylor) Hertel Trapelia placodioides Coppins & P. James Order Candelariales Family Candelariaceae Candelaria concolor (Dicks.) Stein Candelariella aurella (Hoffm.) Zahlbr. Candelariella efflorescens R.C. Harris & W.R. Buck Order Lecanorales Family Acarosporaceae Acarospora glaucocarpa (Ach.) Körb. Family Cladoniaceae Cladonia ochrochlora Flörke Family Lecanoraceae Lecanora allophana (Ach.) Nyl. 1872 Notes: f. sorediata Lecanora allophana f. sorediata Lecanora pulicaris (Pers.) Ach. Lecanora sambuci (Pers.) Nyl. Lecanora symmicta (Ach.) Ach. Lecanora thysanophora R. C. Harris Lecidella carpathica Körb. Family Parmeliaceae Flavoparmelia caperata (Linnaeus) Hale (1986) Flavopunctelia flaventior (Stirt.) Hale Melanelixia subaurifera (Nyl.) O. Blanco et al. Parmelia squarrosa Hale Parmelia sulcata Taylor Punctelia rudecta (Ach.) Krog Punctelia subrudecta (Nyl.) Krog Xanthoparmelia cumberlandia (Gyeln.) Hale, 1974 119 120 Telfer A et al. Family Peltigeraceae Peltigera evansiana Gyelnik Peltigera rufescens (Weiss) Humb. Family Porpidiaceae Bilimbia sabuletorum (Schreb.) Arnold Family Psoraceae Protoblastenia rupestris (Scop.) J. Steiner Psora decipiens (Hedwig) Hoffm. Family Ramalinaceae Bacidia schweinitzii (Fr. ex E. Michener) A. Schneider Lecania croatica (Zahlbr.) Kotlov Lecania naegelii (Hepp) Diederich & v. d. Boom Family Stereocaulaceae Lepraria incana (Linnaeus) Ach. Lepraria lobificans Nyl. Order Lecanoromycetes Family Thelenellaceae Julella fallaciosa (Arnold) R. C. Harris Order Ostropales Family Coenogoniaceae Coenogonium pineti (Ach.) Lücking & Lumbsch, 2004 Family Graphidaceae Graphis scripta (Linnaeus) Ach. (1809) Family Phlyctidaceae Phlyctis spp. Family Stictidaceae Conotrema urceolatum (Ach.) Gilenstam 2005 Order Teloschistales Family Physciaceae Buellia punctata (Hoffm.) A. Massal. Buellia stillingiana J. Steiner 1919 Hyperphyscia adglutinata (Flörke) H. Mayrhofer & Poelt Phaeophyscia adiastola (Essl.) Essl. 1978 Phaeophyscia orbicularis (Neck.) Moberg Phaeophyscia pusilloides (Zahlbr.) Essl. 1978 Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... 121 Phaeophyscia rubropulchra (Degel.) Moberg 1978 Physcia adscendens (Th. Fr.) H. Olivier Physcia aipolia (Ehrh. ex Humb.) Fürnr. Physcia millegrana Degel. Physcia stellaris (Linnaeus) Nyl. Physciella chloantha (Ach.) Essl. Physconia detersa (Nyl.) Poelt Family Teloschistaceae Caloplaca flavovirescens (Wulfen) Søchting, Frödén & Arup Caloplaca pyracea (Ach.) Zwackh Xanthomendoza fallax (Hepp ex Arn.) Soechting, KSrnefelt & S. Kondratyuk Xanthomendoza ulophyllodes (RSsSnen) Soechting, KSrnefelt & S. Kondratyuk Xanthoria parietina (Linnaeus) Th. Fr. Class Leotiomycetes Order Helotiales Family Bulgariaceae Bulgaria inquinans (Pers.) Fr. (1822) Family Helotiaceae Ascocoryne cylichnium (Tul.) Korf 1971 Bisporella citrina (Batsch) Korf & S.E.Carp. (1974) Chlorociboria aeruginascens (Nyl.) Kanouse 1947 Family Hemiphacidiaceae Chlorencoelia versiformis (Pers.) J.R.Dixon (1975) Class Pezizomycetes Order Pezizales Family Pezizaceae Peziza repanda Pers. (1808) Family Pyronemataceae Aleuria aurantia (Pers.) Fuckel, 1870 Jafnea semitosta (Berk. & M.A. Curtis) Korf Scutellinia scutellata (Linnaeus) Lambotte 1887 Scutellinia setosa Nees) Kuntze (1891) Notes: /erinaceous 122 Telfer A et al. Tarzetta cupularis (Linnaeus) Svrček (1981) Class Sordariomycetes Order Hypocreales Family Bionectriaceae Ovicuculispora parmeliae (Berk. & Curt.) Etayo Family Hypocreaceae Hypomyces lactifluorum (Schwein.) Tul. & C. Tul. 1860 Order Xylariales Family Xylariaceae Daldinia concentrica (Bolton) Ces. & De Not. 1863 Hypoxylon fragiforme (Pers.) J. Kickx f. 1835 Ustulina deusta (Hoffm.) P.M.D. Martin, (1970) Notes: (?) Xylaria longipes Nitschke 1867 Xylaria polymorpha (Pers.) Grev. 1824 Phylum Basidiomycota Class Agaricomycetes Order Agaricales Family Agaricaceae Agaricus arvensis Schaeff. 1774 Agaricus placomyces Peck 1878 Calvatia gigantea (Batsch) Lloyd 1904 Coprinus comatus (O.F. Müll.) Pers. 1797 Lepiota clypeolaria (Bull.) P. Kumm. 1871 Lepiota cristata Barla Leucoagaricus naucinus Wasser, 1977 Notes: (Lepiota naucina) Lycoperdon perlatum Persoon,1796 Notes: (Lycoperdon gemmatum) Parasola plicatilis (Curtis) Redhead, Vilgalys & Hopple 2001 Family Amanitaceae Amanita bisporigera G.F.Atk., 1906 Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... 123 Family Bolbitiaceae Conocybe rugosa (Peck) Watling, 1981 Panaeolus foenisecii R.Maire, 1933 Notes: (Panaeolina foenisecii) Family Cortinariaceae Gymnopilus spectabilis (Fr.) A. H. Smith Notes: (Gymnopilus junonius) Phaeomarasmius proximans Singer, 1989 Family Entolomataceae Clitopilus prunulus P. Kumm., 1871 Clitopilus scyphoides Singer, 1946 Entoloma abortivum Donk, 1949 Entoloma spp. Entoloma strictus Hesler (1967) Notes: (Nolanea strictia) Leptonia incana Gillet, ? Notes: Or euchlora Family Hygrophoraceae Arrhenia epichysium (Pers.) Redhead, Lutzoni, Moncalvo & Vilgalys 2002 Hygrocybe coccinea P. Kumm, 1871 Hygrocybe flavescens Singer, 1951 Hygrocybe miniata P. Kumm, 1871 Hygrocybe pratensis Murrill, 1914 Notes: (Camarophyllus pratensis) Hygrocybe punicea P. Kumm, 1871 Hygrocybe virginea P.D. Orton & Watling, 1969 Hygrophorus eburneus Fries, 1838 Family Hymenogastraceae Galerina autumnalis (Peck) A.H. Sm. & Singer Notes: (Galerina marginata) Hebeloma crustuliniforme Bulliard, 1787 Family Inocybaceae Crepidotus applanatus Persoon, 1796 124 Telfer A et al. Crepidotus mollis Staude, 1857 Inocybe rimosa Bulliard, 1789 Inocybe spp. Crepidotus crocophyllus Berkeley, 1847 Family Lycoperdaceae Lycoperdon pyriforme Schaeffer, 1774 Family Lyophyllaceae Lyophyllum decastes Fries, 1818 Family Marasmiaceae Baeospora myosura Fries, 1818 Clitocybula oculus Peck, 1878 Marasmius rotula Scopoli, 1772 Micromphale foetidum (Sowerby) Singer, 1949 Pleurocybella porrigens (Pers.) Singer, 1947 Notes: / Phylotus porrigens Rhodocollybia butryacea (Bulliard, 1792) Lennox, 1979 Notes: (Collybia butryacea) Gymnopus acervatus Fries, 1821 Notes: / Connopus acervatus Xerula furfuracea (Peck, 1893) R. H. Petersen, 2010 Family Mycenaceae Mycena galericulata (Scop.) Gray (1821) Mycena haematopus (Pers.) P.Kummmer, 1871 Mycena inclinata (Fr.) Quél.,1872 Mycena leaiana (Berk.) Saccardo,1891 Mycena olida Bresadola,1887 Mycena osmundicola J. E. Lange, 1914 Mycena spp. Panellus stipticus (Bull.) P.Karst. (1879) Xeromphalina kauffmanii A. H. Smith, 1953 Family Physalacriaceae Armillaria gallica Marxmüller & Romagnesi, 1987 Armillaria mellea complex (Vahl, 1790) Kummer, 1871 Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... 125 Armillaria ostoyae (Romagnesi, 1970) Herink, 1973 Flammulina velutipes (Curtis, 1782) Singer, 1951 Hymenopellis limonispora R.H. Petersen, 2010 Xerula megalospora (Clements, 1896) R. H. Petersen, 2010 Family Pleurotaceae Pleurotus ostreatus (Jacquin) Kummer, 1871 Family Pluteaceae Pluteus atricapillus (Schaeffer) Kummer, 1871 Pluteus atromarginatus (Konrad, 1927) Kühner, 1935 Pluteus cervinus (Schaeffer, 1774) Kummer, 1871 Notes: (Pluteus atricapillus) Pluteus hongoi Singer, 1989 Pluteus longistriatus Peck, 1885 Pluteus umbrosus (Pers.) P. Kummer, 1871 Notes: (Pluteus granularis) Family Psathyrellaceae Coprinellus micaceus (Bull.:Fr.) Vilgalys, Hopple & Jacq. Johnson Coprinopsis atramentaria (Bull.) Redhead, Vilgalys & Moncalvo (2001) Coprinus disseminatus (Pers.) J.E.Lange (1938) Psathyrella candolleana (Fr.) Maire, 1937 Family Schizophyllaceae Schizophyllum commune Fries, 1815 Family Strophariaceae Agrocybe praecox (Pers.) Fayod Gymnopilus penetrans (Fr.) Maire Gymnopolis sapineus (Fr.) Maire Hypholoma capnoides (Fr.) P. Kummer, 1871 Hypholoma fasciculare (Huds.:Fr.) P. Kummer Hypholoma sublateritium (Fr.) Quélet Pholiota aurivella (Batsch) P.Kumm. (1871) Pholiota squarrosa (Batsch) Kummer, 1871 Pholiota squarrosoides (Peck, 1879) Saccardo, 1887 126 Telfer A et al. Family Tricholomataceae Clitocybe ectypoides (Pk) Sacc. Clitocybe gibba (Persoon, 1801) Harmaja, 2003 Collybia cookei (Bres.) J.D.Arnold (1935) Hygrophorus langei (Kühner) A. Pearson, 1952 Notes: (?) Hypsizygus tessulatus (Bull:Fr) Singer Lepista irina (Fr.) H.E. Bigelow 1959 Lepista nuda (Bull.) Cooke 1871 Notes: (Clitocybe nuda) Omphalina spp. Phyllotopsis nidulans (Pers.) Singer 1936 Rhodotus palmatus (Bull.) Maire 1926 Tricholoma aurantium (Schaeff.) Ricken 1914 Tricholoma myomyces (Schaeff.) P. Kumm. 1871 Tricholoma terreum (P.Kumm., 1871) Tricholoma virgatum (P.Kumm., 1871) Xeromphalina cauticinalis (Kühner & Maire, 1934) Panellus serotinus (Kühner,1950) Family Clavariaceae Multiclavula mucida (R.H. Peterson, 1967) Order Boletales Family Boletaceae Boletinellus merulioides (Schwein.) Murrill 1909 Leccinum scabrum Gray 1821 Family Sclerodermataceae Scleroderma areolatum Ehrenberg, 1818 Scleroderma citrinum Persoon, 1801 Scleroderma michiganense (Guzmán) Guzmán 1970 Family Suillaceae Suillus americanus (Peck) Snell (1959) Suillus brevipes (Peck) Kuntze 1898 Suillus granulatus (Linnaeus) Roussel 1796 Suillus luteus (Linnaeus) Roussel 1796 Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Order Cantharellales Family Cantharellaceae Craterellus fallax Smith 1968 Order Corticiales Family Corticiaceae Phlebia tremellosa (Schrader) Nakasone & Burdsall, 1984 Order Geastrales Family Geastraceae Geastrum fimbriatum Fries 1829 Geastrum quadrifidum Persoon, 1794 Order Gomphales Family Gomphaceae Ramaria spp. Ramaria stricta var. concolor (Persoon) Quélet 1888 Order Hymenochaetales Family Hymenochaetaceae Phellinus igniarius (Linnaeus) Quélet 1886 Order Phallales Family Phallaceae Phallus duplicatus Bosc (1811) Phallus impudicus Linnaeus 1753 Phallus ravenelii Berkeley & M.A.Curtis (1873) Order Polyporales Family Meruliaceae Loweomyces fractipes (Berkeley & M.A. Curtis) Jülich 1982 Family Polyporaceae Daedaleopsis confragosa (Bolton) J. Schröter 1888 Fomes fomentarius (Linnaeus) J. Kickx, 1867 Polyporus radicatus Schweinitz, 1832. Polyporus squamosus (Hudson) Fries 1821 Polyporus varius (Persoon) Fries 1821 Trametes gibbosa (Persoon) Fries 1836 Trametes pubescens (Schumacher) Pilát, 1939. Trametes versicolor (Linnaeus) Lloyd 1921 127 128 Telfer A et al. Trichaptum biforme (Fries) Ryvarden, 1972 Truncospora ohiensis (Berkeley) Pilát 1953 Order Russulales Family Hericiaceae Hericium americanum Ginns 1984 Hericium coralloides (Scopoli) Persoon, 1794 Hericium erinaceus (Bulliard) Persoon, 1797. Family Russulaceae Lactarius torminosus (Schaeffer) Gray 1821 Russula rosacea (Persoon) Gray 1821 Russula spp. Family Stereaceae Stereum ostrea (Blume & Nees) Fries, 1838. Stereum rugosum Persoon 1794 Stereum striatum (Fries) Fries 1838 Family Auriscalpiaceae Lentinellus ursinus (Fries) Kühner 1926 Order Thelephorales Family Bankeraceae Boletopsis subsquamosa (Linnaeus) Kotl.& Pouz. Class Basidiomycetes Order Cortinariales Family Cortinariaceae Inocybe geophylla var. geophylla (Persoon) Kummer, 1871 Class Dacrymycetes Order Dacrymycetales Family Dacrymycetaceae Calocera cornea (Batsch) Fries, 1827. Class Tremellomycetes Order Tremellales Family Exidiaceae Exidia alba Lloyd) Burt 1921 Notes: / Ductifera pululahuana Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... 129 Exidia glandulosa (Bulliard) Fries (1822) Family Tremellaceae Tremella foliacea Persoon (1800) Tremella reticulate (Berkeley) Farl. (1908) Class Ustilaginomycetes Order Ustilaginales Family Ustilaginaceae Ustilago maydis (DC.) Corda Phylum Zygomycota Class Zygomycetes Order Mortierellales Family Mortierellaceae Mortierella hyalina (Harz) W. Gams Checklist of species observed or collected at the rare Charitable Research Reserve in Cambridge, Ontario, Canada. The first of two checklists for Kingdom Plantae, this checklist contains records Lycopodiophyta from and Phyla Bryophyta, Magnoliophyta (Class Magnoliopsida). Kingdom Plantae Phylum Bryophyta Class Bryopsida Order Bryales Family Bryaceae Bryum caespiticium Hedw. Bryum argenteum Hedw. Family Mniaceae Mnium marginatum (With.) Brid. ex P. Beauv. Equisetophyta, Liliopsida and 130 Telfer A et al. Family Plagiomniaceae Plagiomnium cuspidatum (Hedw.) T. Kop. Order Dicranales Family Dicranaceae Dicranum flagellare Hedw. Dicranum montanum Hedw. Family Ditrichaceae Ceratodon purpureus (Hedw.) Brid. Ditrichum spp. Family Fissidentaceae Fissidens adianthoides Hedw. Fissidens taxifolius Hedw. Fissidens spp. Family Leucobryaceae Leucobryum glaucum (Hedw.) Ångstr. in Fries Order Encalyptales Family Encalyptaceae Encalypta procera Bruch Order Grimmiales Family Grimmiaceae Schistidium apocarpa (Hedw.) Bsg Schistidium rivulare (Brid.) Podp. Order Hedwigiales Family Hedwigiaceae Hedwigia ciliata (Hedw.) P. Beauv. Order Hypnales Family Amblystegiaceae Amblystegium serpens (Hedw.) Schimp. in B.S.G. Amblystegium varium (Hedw.) Lindb. Campylium chrysophyllum (Brid.) J. Lange Campylium hispidulum (Brid.) Mitt. Campylium spp. Hygroamblystegium fluviatile (Hedw.) Loeske Hygroamblystegium tenax (Hedw.) Jenn. Leptodictyum riparium (Hedw.) Warnst. Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Drepanocladus aduncus (Hedw.) Warnst. Family Anomodontaceae Anomodon attenuatus (Hedw.) Hüb. Anomodon rostratus (Hedw.) Schimp. Family Brachytheciaceae Brachythecium spp. Cirriphyllum piliferum (Hedw.) Grout Eurhynchium hians (Hedw.) Sande Lac. Eurhynchium pulchellum (Hedw.) Jenn. Family Climaciaceae Climacium americanum Brid. Climacium dendroides (Hedw.) Web. & Mohr Family Entodontaceae Entodon seductrix (Hedw.) C. Müll. Family Fontinalaceae Fontinalis hypnoides Hartm. Family Hylocomiaceae Rhytidiadelphus triquetrus (Hedw.) Warnst. Family Hypnaceae Callicladium haldanianum (Grev.) Crum Homomallium adnatum (Hedw.) Broth. Hypnum curvifolium Hedw. Hypnum imponens Hedw. Hypnum lindbergii Mitt. Hypnum spp. Platydictya subtilis (Hedw.) Crum Taxiphyllum deplanatum (Bruch & Schimp. ex Sull.) Fleisch. Family Leskeaceae Leskea polycarpa Hedw. Leskeella nervosa (Brid.) Loeske Family Plagiotheciaceae Isopterygiopsis muelleriana (Schimp.) Iwats. Family Thuidiaceae Thuidium recognitum (Hedw.) Lindb. Thuidium delicatulum (Hedw.) Schimp. in B.S.G. 131 132 Telfer A et al. Order Orthotrichales Family Orthotrichaceae Orthotrichum anomalum Hedw. Order Pottiales Family Pottiaceae Barbula convoluta Hedw. Desmatodon obtusifolius (Schwaegr.) Schimp. Hymenostylium recurvirostre (Hedw.) Dix. Tortella tortuosa (Hedw.) Limpr. Barbula unguiculata Hedw. Tortula ruralis (Hedw.) Gaertn. et al. Weissia spp. Order Splachnales Family Meesiaceae Leptobryum pyriforme (Hedw.) Wils. Class Polytrichopsida Order Polytrichales Family Polytrichaceae Atrichum angustatum (Brid.) Bruch & Schimp. in B.S.G. Atrichum undulatum (Hedw.) P. Beauv. Polytrichum spp. Class Sphagnopsida Order Sphagnales Family Sphagnaceae Sphagnum spp. Class Tetraphidopsida Order Tetraphidales Family Tetraphidaceae Tetraphis pellucida Hedw. Phylum Equisetophyta Class Equisetopsida Order Equisetales Family Equisetaceae Equisetum arvense Linnaeus Equisetum hyemale subsp. affine (Engelm.) Calder & Roy L. Taylor Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... 133 Equisetum palustre Linnaeus Equisetum pratense Ehrh. Equisetum scirpoides Michx. Equisetum sylvaticum Linnaeus Equisetum variegatum subsp. variegatum Schleich. ex F. Weber & D. Mohr Phylum Lycopodiophyta Class Lycopodiopsida Family Huperziaceae Family Huperziaceae Huperzia lucidula (Michx.) Trevis. Order Lycopodiales Family Lycopodiaceae Lycopodiella inundata (Linnaeus) Holub Lycopodium annotinum Linnaeus Lycopodium obscurum Linnaeus Lycopodium ssp. Order Selaginellales Order Selaginellales Family Selaginellaceae Selaginella eclipes W.R. Buck Phylum Magnoliophyta Class Liliopsida Order Alismatales Family Alismataceae Sagittaria latifolia Willd. Sagittaria rigida Pursh Family Araceae Arisaema triphyllum (Linnaeus) Schott Symplocarpus foetidus (Linnaeus) Salisb. ex Nutt. Family Butomaceae Butomus umbellatus Linnaeus Family Potamogetonaceae Potamogeton crispus Linnaeus Stuckenia pectinata (Linnaeus) Börner 134 Telfer A et al. Family Tofieldiaceae Triantha glutinosa (Michx.) Baker Order Arales Family Araceae Calla palustris Linnaeus Lemna minor Linnaeus Wolffia borealis (Engelm. ex Hegelm.) Landolt Wolffia columbiana H. Karst. Order Asparagales Family Amarylilidaceae Allium tricoccum Aiton Family Asparagaceae Asparagus officinalis Linnaeus Convallaria majalis Linnaeus Maianthemum canadense Desf. Maianthemum racemosum subsp. racemosum (Linnaeus) Link Maianthemum stellatum (Linnaeus) Link Family Iridaceae Iris pseudacorus Linnaeus Family Orchidaceae Cypripedium acaule Aiton Cypripedium arietinum R. Br. Cypripedium parviflorum var. pubescens (Willd.) O.W. Knight Cypripedium reginae Walter Order Cyperales Family Cyperaceae Carex albursina E. Sheld. Carex aquatilis Wahlenb. Carex arctata Boott ex Hook. Carex atherodes Spreng. Carex backii Boott Carex bebbii Olney ex Fernald Carex blanda Dewey Carex bromoides Schkuhr ex Willd. Carex chordorrhiza Ehrh. ex L. f. Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Carex comosa Boott Carex crinita Lam. Carex cristatella Britton Carex deweyana Schwein. Carex diandra Schrank Carex disperma Dewey Carex eburnea Boott Carex foenea Willd. Carex formosa Dewey Carex glaucodea Tuck. ex Olney Carex gracillima Schwein. Carex granularis Muhl. ex Willd. Carex grisea Wahlenb. Carex hirtifolia Mack. Carex hitchcockiana Dewey Carex hystericina Muhl. ex Willd. Carex intumescens Rudge Carex jamesii Schwein. Carex lacustris Willd. Carex laevivaginata (Kük.) Mack. Carex lasiocarpa Ehrh. Carex laxiculmis Schwein. Carex laxiflora Lam. Carex leptalea Wahlenb. Carex livida (Wahlenb.) Willd. Carex lupuliformis Sartwell ex Dewey Carex lupulina Muhl. ex Willd. Carex normalis Mack. Carex peckii Howe Carex pedunculata Muhl. ex Willd. Carex pensylvanica Lam. Carex plantaginea Lam. Carex platyphylla J. Carey Carex projecta Mack. Carex pseudocyperus Linnaeus 135 136 Telfer A et al. Carex retrorsa Schwein. Carex rosea Schkuhr ex Willd. Carex scabrata Schwein. Carex schweinitzii Dewey ex Schwein. Carex scoparia Schkuhr ex Willd. Carex sparganioides Muhl. ex Willd. Carex spp. Carex stipata Muhl. ex Willd. Carex stricta Lam. Carex tetanica Schkuhr Carex tonsa var. rugosperma (Mack.) Crins Carex trichocarpa Muhl. ex Willd. Carex trisperma Dewey Carex utriculata Boott Carex vulpinoidea Michx. Carex woodii Dewey Cyperus bipartitus Torr. Cyperus esculentus Linnaeus Dulichium arundinaceum (Linnaeus) Britton Eleocharis acicularis (Linnaeus) Roem. & Schult. Eleocharis intermedia Schult. Eriophorum virginicum Linnaeus Eriophorum viridicarinatum (Engelm.) Fernald Schoenoplectiella smithii var. smithii (A. Gray) Hayasaka Schoenoplectus tabernaemontani (C.C. Gmel.) Palla Scirpus atrovirens Willd. Scirpus cyperinus (Linnaeus) Kunth Order Juncales Family Juncaceae Juniperus virginiana Linnaeus Luzula acuminata Raf. Luzula multiflora subsp. multiflora (Ehrh.) Lej. Order Liliales Family Iridaceae Iris spp. Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Iris versicolor Linnaeus Sisyrinchium angustifolium Mill. Sisyrinchium montanum Greene Sisyrinchium mucronatum Michx. Family Liliaceae Clintonia borealis (Aiton) Raf. Erythronium albidum Nutt. Erythronium americanum subsp. americanum Ker Gawl. Hemerocallis fulva (Linnaeus) L. Hemerocallis lilioasphodelus Linnaeus Lilium canadense Linnaeus Lilium michiganense Farw. Lilium philadelphicum Linnaeus Lilium superbum Linnaeus Medeola virginiana Linnaeus Polygonatum biflorum (Walter) Elliott Polygonatum pubescens (Willd.) Pursh Streptopus lanceolatus var. lanceolatus (Aiton) Reveal Trillium erectum Linnaeus Trillium grandiflorum (Michx.) Salisb. Trillium spp. Uvularia grandiflora Sm. Family Melanthiaceae Anticlea elegans var. glaucus (Nutt.) Zomlefer & Judd Family Smilacaceae Smilax herbacea Linnaeus Smilax tamnoides Linnaeus Order Najadales Family Najadaceae Najas flexilis (Willd.) Rostk. & W.L.E. Schmidt Family Potamogetonaceae Potamogeton amplifolius Tuck. Potamogeton pusillus subsp. pusillus Linnaeus Order Orchidales Family Orchidaceae 137 138 Telfer A et al. Aplectrum hyemale (Muhl. ex Willd.) Torr. Epipactis helleborine (Linnaeus) Crantz Galearis spectabilis (Linnaeus) Raf. Liparis loeselii (Linnaeus) Rich. Platanthera dilatata (Pursh) Lindl. ex L.C. Beck Platanthera hyperborea (Linnaeus) Lindl. Platanthera nivea (Nutt.) Luer Spiranthes lucida (H.H. Eaton) Ames Order Poales Family Juncaceae Juncus acuminatus Michx. Juncus articulatus Linnaeus Juncus brachycarpus Engelm. Juncus bufonius Linnaeus Juncus dudleyi Wiegand Juncus effusus Linnaeus Juncus nodosus Linnaeus Juncus tenuis Willd. Family Poaceae Agrostis capillaris Linnaeus Agrostis gigantea Roth Agrostis stolonifera Linnaeus Alopecurus aequalis Sobol. Andropogon gerardii Vitman Avena fatua Linnaeus Avena sativa Linnaeus Brachyelytrum erectum (Schreb.) P. Beauv. Briza maxima Linnaeus Bromus ciliatus Linnaeus Bromus latiglumis (Scribn. ex Shear) Hitchc. Bromus pubescens Muhl. ex Willd. Calamagrostis canadensis (Michx.) P. Beauv. Cinna arundinacea Linnaeus Dactylis glomerata Linnaeus Danthonia spicata (Linnaeus) P. Beauv. ex Roem. & Schult. Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... 139 Dichanthelium dichotomum var. dichotomum (Linnaeus) Gould Dichanthelium latifolium (Linnaeus) Harvill Dichanthelium linearifolium (Scribn.) Gould Digitaria sanguinalis (Linnaeus) Scop. Eleusine indica (Linnaeus) Gaertn. Elymus canadensis Linnaeus Elymus hystrix Linnaeus Elymus lanceolatus subsp. psammophilus (J.M. Gillett & H. Senn) Á. Löve Elymus repens (Linnaeus) Gould Elymus riparius Wiegand Elymus virginicus var. virginicus Linnaeus Festuca rubra subsp. rubra Linnaeus Glyceria borealis (Nash) Batch. Glyceria canadensis (Michx.) Trin. Glyceria maxima (Hartm.) Holmb. Glyceria septentrionalis Hitchc. Glyceria striata (Lam.) Hitchc. Leersia oryzoides (Linnaeus) Sw. Lolium perenne Linnaeus Milium effusum Linnaeus Muhlenbergia glomerata (Willd.) Trin. Muhlenbergia mexicana var. mexicana (Linnaeus) Trin. Muhlenbergia tenuiflora (Willd.) Britton, Sterns & Poggenb. Oryzopsis asperifolia Michx. Panicum capillare Linnaeus Panicum virgatum Linnaeus Patis racemosa (Smith) Romaschenko, P.M. Peterson & Soreng Phalaris arundinacea Linnaeus Poa compressa Linnaeus Poa nemoralis Linnaeus Poa pratensis subsp. pratensis Linnaeus Poa ssp. Schizachne purpurascens (Torr.) Swallen Schizachyrium scoparium (Michx.) Nash 140 Telfer A et al. Setaria viridis (Linnaeus) P. Beauv. Sorghastrum nutans (Linnaeus) Nash Sporobolus vaginiflorus (Torr. ex A. Gray) Alph. Wood Bromus inermis Leysser Bromus kalmii A. Gray Echinochloa crus-galli (Linnaeus) P. Beauv. Phleum pratense Linnaeus Phragmites australis (Cav.) Trin. ex Steud. Setaria pumila (Poir.) Roem. & Schult. Spartina pectinata Link Family Typhaceae Typha angustifolia Linnaeus Typha latifolia Linnaeus Order Typhales Family Sparganiaceae Sparganium americanum Nutt. Sparganium eurycarpum Engelm. Class Magnoliopsida Order Apiales Family Apiaceae Aegopodium podagraria Linnaeus Angelica atropurpurea Linnaeus Cicuta bulbifera Linnaeus Cicuta maculata Linnaeus Conioselinum chinense (Linnaeus) Britton, Sterns & Poggenb. Cryptotaenia canadensis (Linnaeus) DC. Daucus carota Linnaeus Erigenia bulbosa (Michx.) Nutt. Heracleum mantegazzianum Sommier & Levier Osmorhiza claytonii (Michx.) C.B. Clarke Sanicula canadensis var. grandis Fernald Sanicula marilandica Linnaeus Sium suave Walter Thaspium trifoliatum var. aureum (Linnaeus) Britton Zizia aurea (Linnaeus) W.D.J. Koch Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Family Araliaceae Aralia elata (Miq.) Seem. Aralia nudicaulis Linnaeus Aralia racemosa subsp. racemosa Linnaeus Hydrocotyle americana Linnaeus Panax quinquefolius Linnaeus Panax trifolius Linnaeus Order Aristolochiales Family Aristolochiaceae Asarum canadense Linnaeus Order Asterales Family Asteraceae Achillea millefolium Linnaeus Ageratina altissima (Linnaeus) King & H. Rob. Ambrosia artemisiifolia Linnaeus Ambrosia trifida Linnaeus Antennaria neglecta Greene Arctium lappa Linnaeus Arctium minus Bernh. Artemisia campestris subsp. caudata (Michx.) H.M. Hall & Clem. Artemisia vulgaris Linnaeus Aster spp. Bidens cernua Linnaeus Bidens connata Muhl. ex Willd. Bidens frondosa Linnaeus Bidens ssp. Bidens vulgata Greene Carduus nutans Linnaeus Centaurea jacea Linnaeus Centaurea stoebe subsp. micranthos (Gugler) Hayek Cichorium intybus Linnaeus Cirsium arvense (Linnaeus) Scop. Cirsium discolor (Muhl. ex Willd.) Spreng. Cirsium muticum Michx. Cirsium oleraceum (Linnaeus) Scop. 141 142 Telfer A et al. Cirsium pumilum var. hilii (Canby) B. Boivin Cirsium vulgare (Savi) Ten. Echinacea purpurea (Linnaeus) Moench Erechtites hieraciifolius (Linnaeus) Rafinesque ex de Candolle Erigeron annuus (Linnaeus) Pers. Erigeron canadensis Linnaeus Erigeron philadelphicus var. philadelphicus Linnaeus Erigeron pulchellus Michx. Erigeron strigosus Muhl. ex Willd. Eupatorium hyssopifolium Linnaeus Eupatorium perfoliatum Linnaeus Eurybia macrophylla (Linnaeus) Cass. Euthamia graminifolia (Linnaeus) Nutt. Eutrochium maculatum var. maculatum (Linnaeus) E.E. Lamont Eutrochium purpureum var. purpureum (Linnaeus) E.E. Lamont Helenium flexuosum Raf. Helianthus annuus Linnaeus Helianthus decapetalus Linnaeus Helianthus giganteus Linnaeus Helianthus tuberosus Linnaeus Heliopsis helianthoides (Linnaeus) Sweet Hieracium umbellatum Linnaeus Hieracium vulgatum Fr. Inula helenium Linnaeus Lactuca canadensis Linnaeus Lactuca serriola Linnaeus Lapsana communis Linnaeus Leucanthemum vulgare Lam. Liatris cylindracea Michx. Matricaria discoidea DC. Nabalus albus (Linnaeus) Hooker Nabalus altissimus (Linnaeus) Hooker Onopordum acanthium Linnaeus Packera aurea (Linnaeus) Á. Löve & D. Löve Pilosella ×floribunda (Wimmer & Grabowski) Fries Notes: X floribundum Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... 143 Pilosella aurantiaca (Linnaeus) F.W. Schultz & Schultz Bipontinus Pilosella caespitosa (Dumortier) P.D. Sell & C. West Pilosella officinarum F.W. Schultz & Schultz Bipontinus Pilosella piloselloides subsp. piloselloides (Villars) Soják Pilosella piloselloides subsp. Greuter praealta (Gochnat) S. Bräutigam & Prenanthes spp. Ratibida pinnata (Vent.) Barnhart Rudbeckia hirta Linnaeus Rudbeckia laciniata Linnaeus Solidago altissima var. altissima Linnaeus Solidago caesia Linnaeus Solidago canadensis Linnaeus Solidago flexicaulis Linnaeus Solidago gigantea Aiton Solidago hispida Muhl. ex Willd. Solidago juncea Aiton Solidago nemoralis subsp. nemoralis Aiton Solidago patula Muhl. ex Willd. Solidago ptarmicoides (Torr. & A. Gray) B. Boivin Solidago rigida Linnaeus Solidago rugosa subsp. rugosa Mill. Solidago spp. Solidago ulmifolia Muhl. ex Willd. Sonchus arvensis subsp. arvensis Linnaeus Sonchus asper (Linnaeus) Hill Sonchus oleraceus Linnaeus Symphyotrichum cordifolium (Linnaeus) G.L. Nesom Symphyotrichum ericoides var. ericoides (Linnaeus) G.L. Nesom Symphyotrichum laeve (Linnaeus) Á. Löve & D. Löve Symphyotrichum lanceolatum (Willd.) G.L. Nesom Symphyotrichum lateriflorum var. lateriflorum (Linnaeus) Á. Löve & D. Löve Symphyotrichum lateriflorum var. hirsuticaule (Lindl. ex DC.) G.L. Nesom Symphyotrichum novae-angliae (Linnaeus) G.L. Nesom 144 Telfer A et al. Symphyotrichum novi-belgii (Linnaeus) G.L. Nesom Symphyotrichum oolentangiense var. Nesom oolentangiense (Riddell) G.L. Symphyotrichum oolentangiense (Riddell) G.L. Nesom Symphyotrichum pilosum var. pilosum (Willd.) G.L. Nesom Symphyotrichum puniceum (Linnaeus) Á. Löve & D. Löve Symphyotrichum racemosum (Elliot) G.L. Nesom Symphyotrichum spp. Symphyotrichum urophyllum (Lindl.) G.L. Nesom Tanacetum vulgare Linnaeus Taraxacum officinale F.H. Wigg. Tragopogon dubius Scop. Tragopogon pratensis Linnaeus Tussilago farfara Linnaeus Xanthium strumarium Linnaeus Family Campanulaceae Lobelia siphilitica Linnaeus Order Boraginales Family Boraginaceae Hackelia virginiana (Linnaeus) I.M. Johnst. Myosotis scorpioides Linnaeus Order Brassicales Family Brassicaceae Hesperis matronalis Linnaeus Rorippa sylvestris (Linnaeus) Besser Order Campanulales Family Campanulaceae Campanula rapunculoides Linnaeus Campanula rotundifolia Linnaeus Lobelia cardinalis Linnaeus Lobelia inflata Linnaeus Order Capparales Family Brassicaceae Alliaria petiolata (M. Bieb.) Cavara & Grande Notes: or petiolata Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Barbarea vulgaris W.T. Aiton Berteroa incana (Linnaeus) DC. Brassica juncea (Linnaeus) Czern. Brassica rapa Linnaeus Capsella bursa-pastoris (Linnaeus) Medik. Cardamine bulbosa (Schreb. ex Muhl.) Britton, Sterns & Poggenb. Cardamine concatenata (Michx.) Sw. Cardamine diphylla (Michx.) Alph. Wood Cardamine maxima (Nutt.) Alph. Wood Cardamine pensylvanica Muhl. ex Willd. Erysimum cheiranthoides Linnaeus Nasturtium officinale W.T. Aiton Rorippa palustris subsp. palustris Linnaeus Sinapis alba Linnaeus Sinapis arvensis Linnaeus Sisymbrium altissimum Linnaeus Thlaspi arvense Linnaeus Turritis glabra Linnaeus Order Caryophyllales Family Amaranthaceae Amaranthus albus Linnaeus Amaranthus retroflexus Linnaeus Amaranthus tuberculatus (Moq.) J.D. Sauer Family Caryophyllaceae Arenaria serpyllifolia Linnaeus Cerastium arvense subsp. arvense Linnaeus Cerastium fontanum subsp. vulgare (Hartm.) Greuter & Burdet Dianthus armeria Linnaeus Dianthus barbatus Linnaeus Saponaria officinalis Linnaeus Silene latifolia (Mill.) Britten & Rendle Silene vulgaris (Moench) Garcke Stellaria graminea Linnaeus Family Chenopodiaceae Chenopodium album Linnaeus 145 146 Telfer A et al. Family Phytolaccaceae Phytolacca americana var. americana Linnaeus Family Polygonaceae Persicaria hydropiper (Linnaeus) Opiz Persicaria pensylvanica (Linnaeus) M. Gómez Rumex britannica Linnaeus Rumex crispus Linnaeus Rumex obtusifolius Linnaeus Rumex verticillatus Linnaeus Family Portulacaceae Claytonia virginica Linnaeus Order Celastrales Family Aquifoliaceae Ilex verticillata (Linnaeus) A. Gray Family Celastraceae Celastrus scandens Linnaeus Euonymus alatus (Thunb.) Siebold Euonymus atropurpureus Jacq. Euonymus obovatus Nutt. Order Cornales Family Cornaceae Cornus alternifolia L. f. Cornus canadensis Linnaeus Cornus obliqua Raf. Cornus racemosa Lam. Cornus rugosa Lam. Cornus stolonifera Michx. Order Cucurbitales Family Cucurbitaceae Echinocystis lobata (Michx.) Torr. & A. Gray Order Dipsacales Family Adoxaceae Viburnum acerifolium Linnaeus Viburnum lantana Linnaeus Viburnum lentago Linnaeus Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Viburnum opulus Linnaeus Viburnum opulus var. americanum Aiton Viburnum rafinesquianum Schult. Family Caprifoliaceae Caprifoliaceae spp. Diervilla lonicera Mill. Dipsacus sativus (Linnaeus) Honck. Linnaea borealis subsp. longiflora (Torr.) Hultén Lonicera canadensis Bartram & W. Bartram ex Marshall Lonicera dioica Linnaeus Lonicera morrowii A. Gray Lonicera tatarica Linnaeus Sambucus canadensis Linnaeus Sambucus nigra Linnaeus Sambucus racemosa subsp. pubens (Michx.) House Symphoricarpos albus (Linnaeus) S.F. Blake Symphoricarpos occidentalis Hook. Triosteum aurantiacum E.P. Bicknell Family Dipsacaceae Dipsacus fullonum Linnaeus Family Valerianaceae Valeriana edulis subsp. cilata (Torrey & A. Gray) F.G. Meyer Order Ericales Family Balsaminaceae Impatiens capensis Meerb. Family Ericaceae Epigaea repens Linnaeus Gaultheria hispidula (Linnaeus) Muhl. ex Bigelow Gaultheria procumbens Linnaeus Vaccinium angustifolium Aiton Vaccinium myrtilloides Michx. Family Primulaceae Lysimachia arvensis (Linnaeus) U. Manns & Anderberg Family Pyrolaceae Pyrola elliptica Nutt. 147 148 Telfer A et al. Pyrola spp. Order Euphorbiales Family Euphorbiaceae Euphorbia cyparissias Linnaeus Euphorbia esula Linnaeus Checklist of species observed or collected at the rare Charitable Research Reserve in Cambridge, Ontario, Canada. The second of two checklists for Kingdom Plantae, this checklist contains records from Phyla Magnoliophyta (Class Magnoliopsida), Marchantiophyta, Pinophyta, and Pteridophyta. Phylum Magnoliophyta Kingdom Plantae Class Magnoliopsida Order Fabales Family Fabaceae Amorpha canescens Pursh Amphicarpaea bracteata (Linnaeus) Fernald Apios americana Medik. Desmodium canadense (Linnaeus) DC. Glycyrrhiza lepidota Pursh Lespedeza capitata Michx. Lotus corniculatus Linnaeus Medicago lupulina Linnaeus Medicago sativa subsp. sativa Linnaeus Melilotus albus Medik. Robinia pseudoacacia Linnaeus Trifolium aureum Pollich Trifolium campestre Schreb. Trifolium hybridum Linnaeus Trifolium pratense Linnaeus Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Trifolium repens Linnaeus Vicia americana Muhl. ex Willd. Vicia cracca Linnaeus Order Fagales Family Betulaceae Alnus glutinosa (Linnaeus) Gaertn. Alnus incana subsp. rugosa (Du Roi) R.T. Clausen Betula alleghaniensis Britton Betula papyrifera Marshall Betula pendula Roth Betula populifolia Marshall Betula pumila Linnaeus Carpinus caroliniana subsp. virginiana (Marshall) Furlow Corylus americana Walter Corylus cornuta subsp. cornuta Marshall Ostrya virginiana (Mill.) K. Koch Family Fagaceae Fagus grandifolia Ehrh. Quercus alba Linnaeus Quercus bicolor Willd. Quercus ellipsoidalis E.J. Hill Quercus macrocarpa Michx. Quercus palustris Münchh. Quercus rubra Linnaeus Quercus velutina Lam. Family Juglandaceae Juglans nigra Linnaeus Order Gentianales Family Apocynaceae Apocynum androsaemifolium Linnaeus Apocynum cannabinum Linnaeus Apocynum sp. Notes: X floribundum 149 150 Telfer A et al. Vinca minor Linnaeus Family Asclepiadaceae Asclepias exaltata Linnaeus Asclepias incarnata subsp. incarnata Linnaeus Asclepias syriaca Linnaeus Asclepias tuberosa Linnaeus Family Gentianaceae Gentiana andrewsii Griseb. Gentiana rubricaulis Schwein. Gentianella quinquefolia subsp. quinquefolia (Linnaeus) Small Gentianopsis crinita (Froel.) Ma Gentianopsis virgata subsp. virgata (Raf.) Holub Notes: or virgata Family Rubiaceae Galium aparine Linnaeus Galium asprellum Michx. Order Geraniales Family Balsaminaceae Impatiens glandulifera Royle Impatiens pallida Nutt. Family Geraniaceae Geranium maculatum Linnaeus Geranium robertianum Linnaeus Family Oxalidaceae Oxalis corniculata Linnaeus Oxalis montana Raf. Oxalis stricta Linnaeus Order Haloragales Family Haloragaceae Myriophyllum heterophyllum Michx. Myriophyllum verticillatum Linnaeus Order Hamamelidales Family Platanaceae Platanus occidentalis Linnaeus Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Order Juglandales Family Juglandaceae Carya cordiformis (Wangenh.) K. Koch Carya glabra (Mill.) Sweet Carya ovata var. ovata (Mill.) K. Koch Juglans cinerea Linnaeus Order Lamiales Family Boraginaceae Anchusa arvensis (Linnaeus) M. Bieb. Cynoglossum officinale Linnaeus Echium vulgare Linnaeus Lithospermum parviflorum Weakley, Witsell & D. Estes Myosotis laxa Lehm. Myosotis verna Nutt. Symphytum officinale Linnaeus Family Lamiaceae Agastache nepetoides (Linnaeus) Kuntze Ajuga reptans Linnaeus Blephilia hirsuta (Pursh) Benth. Clinopodium vulgare Linnaeus Collinsonia canadensis Linnaeus Galeopsis tetrahit Linnaeus Glechoma hederacea Linnaeus Lamium amplexicaule Linnaeus Leonurus cardiaca Linnaeus Lycopus americanus Muhl. ex W.P.C. Bartram Lycopus asper Greene Lycopus uniflorus Michx. Melissa officinalis Linnaeus Mentha arvensis auct. non L. Mentha canadensis Linnaeus Mentha sp. Notes: X piperita 151 152 Telfer A et al. Mentha spicata Linnaeus Monarda didyma Linnaeus Monarda fistulosa Sims Nepeta cataria Linnaeus Physostegia virginiana subsp. virginiana (Linnaeus) Benth. Prunella vulgaris subsp. vulgaris Linnaeus Prunella vulgaris subsp. lanceolata (W.P.C. Barton) Piper & Beattie Prunella vulgaris Linnaeus Pycnanthemum virginianum (Linnaeus) Rob. & Fernald Scutellaria galericulata Linnaeus Scutellaria lateriflora Linnaeus Stachys hispida Pursh Stachys palustris Linnaeus Teucrium canadense subsp. canadense Linnaeus Family Oleaceae Ligustrum vulgare Linnaeus Syringa vulgaris Linnaeus Family Plantaginaceae Linaria vulgaris Mill. Plantago lanceolata Linnaeus Plantago major Linnaeus Plantago rugelii Decne. Veronica anagallis-aquatica Linnaeus Family Scrophulariaceae Verbascum phoeniceum Linnaeus Verbascum thapsus Linnaeus Veronica chamaedrys Linnaeus Veronica officinalis Linnaeus Veronica serpyllifolia Linnaeus Family Verbenaceae Verbena hastata Linnaeus Verbena simplex Lehm. Verbena stricta Vent. Verbena urticifolia Linnaeus Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Order Laurales Family Lauraceae Lindera benzoin (Linnaeus) Blume Order Linales Family Linaceae Linum virginianum Linnaeus Order Magnoliales Family Annonaceae Asimina triloba (Linnaeus) Dunal Order Malpighiales Family Hypericaceae Hypericum ascyron Linnaeus Hypericum mutilum subsp. mutilum Linnaeus Hypericum perforatum Linnaeus Hypericum punctatum Lam. Family Salicaceae Populus alba Linnaeus Populus balsamifera Linnaeus Populus deltoides W. Bartram ex Marshall Populus grandidentata Michx. Populus nigra Linnaeus Populus tremuloides Michx. Order Malvales Family Malvaceae Abutilon theophrasti Medik. Malva moschata Linnaeus Family Tiliaceae Tilia americana Linnaeus Order Myrtales Family Lythraceae Lythrum salicaria Linnaeus Family Onagraceae Chamerion angustifolium subsp. angustifolium (Linnaeus) Holub Circaea alpina Linnaeus Circaea canadensis subsp. canadensis (Linnaeus) Hill 153 154 Telfer A et al. Epilobium ciliatum subsp. glandulosum (Lehm.) Hoch & P.H. Raven Epilobium coloratum Biehler Epilobium hirsutum Linnaeus Epilobium leptophyllum Raf. Epilobium strictum Muhl. Ludwigia polycarpa Short & Peter Oenothera biennis Linnaeus Oenothera serrulata Nuttall Family Thymelaeaceae Dirca palustris Linnaeus Order Nymphaeales Family Cabombaceae Brasenia schreberi J.F. Gmel. Order Papaverales Family Papaveraceae Chelidonium majus Linnaeus Dicentra canadensis (Goldie) Walp. Dicentra cucullaria (Linnaeus) Bernh. Sanguinaria canadensis Linnaeus Order Piperales Family Saururaceae Saururus cernuus Linnaeus Order Plantaginales Family Plantaginaceae Callitriche palustris Linnaeus Chelone glabra Linnaeus Digitalis lanata Ehrh. Digitalis lutea Linnaeus Notes: or grandiflora Digitalis purpurea Linnaeus Order Polygalales Family Polygalaceae Polygala spp Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Polygala verticillata var. verticillata Linnaeus Polygaloides paucifolia (Willdenow) J.R. Abbott Order Polygonales Family Polygonaceae Fallopia convolvulus (Linnaeus) Á. Löve Fallopia scandens (Linnaeus) Holub Persicaria hydropiperoides (Michx.) Small Persicaria maculosa Gray Persicaria punctata (Elliott) Small Persicaria sagittata (Linnaeus) H. Gross Polygonum aviculare Linnaeus Order Primulales Family Primulaceae Lysimachia borealis (Rafinesque) U. Manns & Anderberg Lysimachia ciliata Linnaeus Lysimachia nummularia Linnaeus Lysimachia thyrsiflora Linnaeus Primula mistassinica Michx. Order Ranunculales Family Berberidaceae Berberis canadensis Mill. Berberis thunbergii DC. Berberis vulgaris Linnaeus Caulophyllum thalictroides (Linnaeus) Michx. Podophyllum peltatum Linnaeus Family Menispermaceae Menispermum canadense Linnaeus Family Ranunculaceae Actaea pachypoda Elliott Actaea rubra (Aiton) Willd. Actaea spp. Anemone acutiloba (DC.) G. Lawson Anemone americana (DC.) H. Hara Anemone canadensis Linnaeus Anemone cylindrica A. Gray 155 156 Telfer A et al. Anemone quinquefolia Linnaeus Anemone virginiana var. virginiana Linnaeus Anemone virginiana var. alba (Oakes) Alph. Wood Aquilegia canadensis Linnaeus Caltha palustris Linnaeus Clematis virginiana Linnaeus Coptis trifolia (Linnaeus) Salisb. Ranunculus abortivus Linnaeus Ranunculus acris Linnaeus Ranunculus hispidus var. caricetorum (Greene) T. Duncan Ranunculus recurvatus var. recurvatus Poir. Ranunculus repens Linnaeus Ranunculus sceleratus var. sceleratus Linnaeus Thalictrum dasycarpum Fisch. & Avé-Lall. Thalictrum dioicum Linnaeus Thalictrum pubescens Pursh Thalictrum thalictroides (Linnaeus) Eames & B. Boivin Order Rhamnales Family Elaeagnaceae Elaeagnus angustifolia Linnaeus Elaeagnus umbellata Thunb. Shepherdia canadensis (Linnaeus) Nutt. Family Rhamnaceae Frangula alnus Mill. Rhamnus alnifolia L'Hér. Order Rosales Family Cannabaceae Humulus lupulus Linnaeus Family Crassulaceae Sedum acre Linnaeus Family Grossulariaceae Ribes americanum Mill. Ribes cynosbati Linnaeus Ribes glandulosum Grauer Ribes hirtellum Michx. Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Ribes lacustre (Pers.) Poir. Ribes oxyacanthoides var. oxycanthoides Linnaeus Ribes rubrum Linnaeus Ribes ssp. Ribes triste Pall. Family Hydrangeaceae Philadelphus coronarius Linnaeus Family Rhamnaceae Rhamnus cathartica Linnaeus Family Rosaceae Agrimonia gryposepala Wallr. Amelanchier arborea (F. Michx.) Fernald Amelanchier laevis Wiegand Amelanchier spicata (Lam.) K. Koch Aruncus dioicus (Walter) Fernald Crataegus chrysocarpa Ashe Crataegus crus-galli Linnaeus Crataegus dissona Sarg. Crataegus punctata Jacq. Crataegus spp. Dasiphora fruticosa auct. non (Linnaeus) Rydb. Fragaria vesca Linnaeus Fragaria virginiana Duchesne Geum aleppicum Jacq. Geum canadense Jacq. Geum fragarioides (Michaux) Smedmark Geum laciniatum Murray Geum rivale Linnaeus Geum urbanum Linnaeus Malus coronaria (Linnaeus) Mill. Malus pumila Mill. Physocarpus opulifolius (Linnaeus) Maxim. Potentilla anserina subsp. anserina Linnaeus Potentilla argentea Linnaeus Potentilla norvegica Linnaeus 157 158 Telfer A et al. Potentilla recta Linnaeus Potentilla simplex Michx. Prunus americana Marshall Prunus avium (Linnaeus) L. Prunus mahaleb Linnaeus Prunus pensylvanica L. f. Prunus serotina Ehrh. Prunus virginiana subsp. virginiana Linnaeus Pyrus communis Linnaeus Rosa blanda Aiton Rosa cinnamomea L. sensu 1759, non 1753 Rosa multiflora Thunb. Rosa palustris Marshall Rosa rugosa Thunb. Rosa virginiana Mill. Rubus allegheniensis Porter Rubus canadensis Linnaeus Rubus flagellaris Willd. Rubus hispidus Linnaeus Rubus idaeus Linnaeus Rubus occidentalis Linnaeus Rubus pubescens Raf. Rubus repens (Linnaeus) Kuntze Sorbus americana Marshall Sorbus aucuparia Linnaeus Spiraea alba Du Roi Family Saxifragaceae Micranthes virginiensis (Michx.) Small Mitella diphylla Linnaeus Mitella nuda Linnaeus Parnassia glauca Raf. Tiarella cordifolia Linnaeus Order Rubiales Family Rubiaceae Galium circaezans Michx. Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Galium mollugo Linnaeus Galium palustre Linnaeus Galium tinctorium Linnaeus Galium trifidum subsp. trifidum Linnaeus Galium triflorum Michx. Mitchella repens Linnaeus Order Salicales Family Salicaceae Salix ×fragilis Linnaeus Salix alba Linnaeus Salix amygdaloides Andersson Salix bebbiana Sarg. Salix candida Flüggé ex Willd. Salix cordata Muhl. Salix discolor Muhl. Salix eriocephala Michx. Salix exigua Nutt. Salix humilis var. humilis Marshall Salix lucida Muhl. Salix nigra Marshall Salix pedicellaris Pursh Salix petiolaris Sm. Salix purpurea Linnaeus Salix serissima (L.H. Bailey) Fernald Salix viminalis Linnaeus Order Sapindales Family Aceraceae Acer nigrum F. Michaux Acer platanoides Linnaeus Acer rubrum Linnaeus Acer saccharinum Linnaeus Acer saccharum Marshall Acer spicatum Lam. Family Anacardiaceae Rhus aromatica Aiton 159 160 Telfer A et al. Rhus typhina Linnaeus Toxicodendron radicans subsp. negundo (Greene) Gillis Toxicodendron radicans var. rydbergii (Small ex Rydb.) Erskine Toxicodendron vernix (Linnaeus) Kuntze Family Rutaceae Zanthoxylum americanum Mill. Family Sapindaceae Acer negundo Linnaeus Aesculus hippocastanum Linnaeus Family Staphyleaceae Staphylea trifolia Linnaeus Order Saxifragales Family Haloragaceae Myriophyllum sibiricum Kom. Order Scrophulariales Family Oleaceae Fraxinus americana Linnaeus Fraxinus nigra Marsh. Fraxinus pennsylvanica Marshall Family Orobanchaceae Conopholis americana (Linnaeus) Wallr. Epifagus virginiana (Linnaeus) W.P.C. Barton Family Scrophulariaceae Aureolaria flava (Linnaeus) Farw. Aureolaria virginica (Linnaeus) Pennell Pedicularis canadensis Linnaeus Penstemon digitalis Nutt. ex Sims Penstemon hirsutus (Linnaeus) Willd. Scrophularia lanceolata Pursh Scrophularia marilandica Linnaeus Verbascum blattaria Linnaeus Veronica americana Schwein. ex Benth. Order Solanales Family Convolvulaceae Calystegia spithamaea (Linnaeus) Pursh Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Convolvulus arvensis Linnaeus Cuscuta gronovii Willd. ex Schult. Family Hydrophyllaceae Hydrophyllum canadense Linnaeus Hydrophyllum virginianum Linnaeus Family Polemoniaceae Phlox subulata subsp. subulata Linnaeus Family Solanaceae Physalis heterophylla Nees Solanum dulcamara Linnaeus Solanum lycopersicum Linnaeus Order Urticales Family Moraceae Morus alba Linnaeus Family Ulmaceae Celtis occidentalis Linnaeus Ulmus americana Linnaeus Ulmus pumila Linnaeus Ulmus rubra Muhl. Ulmus thomasii Sarg. Family Urticaceae Boehmeria cylindrica (Linnaeus) Sw. Laportea canadensis (Linnaeus) Wedd. Pilea pumila (Linnaeus) A. Gray Urtica dioica subsp. gracilis (Aiton) Selander Urtica dioica subsp. dioica Linnaeus Order Violales Family Cucurbitaceae Sicyos angulatus Linnaeus Family Violaceae Viola adunca var. adunca Sm. Viola canadensis Linnaeus Viola cucullata Aiton Viola labradorica Schrank Viola macloskeyi F.E. Lloyd 161 162 Telfer A et al. Viola nephrophylla Greene Viola pubescens Aiton Viola renifolia A. Gray Viola rostrata Pursh Viola sororia Willd. Viola sororia var. affinis (Leconte) L.E. McKinney Viola spp. Order Vitales Family Vitaceae Parthenocissus quinquefolia (Linnaeus) Planch. Vitis riparia Michx. Order Lamiales Family Phrymaceae Phryma leptostachya Linnaeus Order Saxifragales Family Hamamelidaceae Hamamelis virginiana Linnaeus Phylum Marchantiophyta Class Jungermanniopsida Order Metzgeriales Family Aneuraceae Aneura pinguis (Linnaeus) Dumort. Order Jungermanniales Family Geocalycaceae Lophocolea heterophylla (Schrad.) Dum. Family Porellaceae Porella platyphylla (Linnaeus) Pfeiff. Family Radulaceae Radula complanata (Linnaeus) Dum. Order Porellales Family Frullaniaceae Frullania eboracensis Gottsche Class Marchantiopsida Order Marchantiales Family Aytoniaceae Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... Reboulia hemisphaerica (Linnaeus) Raddi Family Conocephalaceae Conocephalum conicum (Linnaeus) Lindb. Family Marchantiaceae Marchantia polymorpha Linnaeus Phylum Pinophyta Class Pinopsida Order Cupressales Family Cupressaceae Juniperus communis Linnaeus Juniperus communis var. depressa Pursh Order Pinales Family Cupressaceae Thuja occidentalis Linnaeus Family Pinaceae Larix decidua Mill. Larix laricina (Du Roi) K. Koch Picea abies (Linnaeus) Karst. Picea glauca (Moench) Voss Picea pungens Engelm. Pinus banksiana Lamb. Pinus resinosa Aiton Pinus strobus Linnaeus Pinus sylvestris Linnaeus Tsuga canadensis (Linnaeus) Carrière Phylum Pteridophyta Class Filicopsida Order Ophioglossales Family Ophioglossaceae Botrypus virginianus (Linnaeus) Michaux Order Polypodiales Family Aspleniaceae Asplenium platyneuron (Linnaeus) Britton, Sterns & Poggenb. Asplenium trichomanes subsp. trichomanes Linnaeus Asplenium viride Huds. 163 164 Telfer A et al. Family Dennstaedtiaceae Pteridium aquilinum var. latiusculum (Desv.) Underw. ex A. Heller Family Dryopteridaceae Athyrium filix-femina var. angustum (Willd.) G. Lawson Cystopteris bulbifera (Linnaeus) Bernh. Cystopteris fragilis (Linnaeus) Bernh. Notes: or tenuis Deparia acrostichoides (Sw.) M. Kato Dryopteris ×triploidea Wherry Dryopteris carthusiana (Vill.) H.P. Fuchs Notes: or spinulosa Dryopteris clintoniana (D.C. Eaton) Dowell Dryopteris cristata (Linnaeus) A. Gray Dryopteris goldiana (Hook. ex Goldie) A. Gray Dryopteris intermedia (Muhl. ex Willd.) A. Gray Dryopteris marginalis (Linnaeus) A. Gray Gymnocarpium dryopteris (Linnaeus) Newman Matteuccia struthiopteris var. pensylvanica (Willd.) C.V. Morton Onoclea sensibilis Linnaeus Polystichum acrostichoides (Michx.) Schott Family Osmundaceae Osmunda claytoniana Linnaeus Osmunda regalis var. spectabilis (Willd.) A. Gray Family Polypodiaceae Polypodium virginianum Linnaeus Notes: or vulgare Family Pteridaceae Adiantum pedatum Linnaeus Pellaea glabella Mett. ex Kuhn Family Thelypteridaceae Thelypteris noveboracensis (Linnaeus) Nieuwl. Thelypteris palustris var. pubescens (G. Lawson) Fernald Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... 165 Class Polypodiopsida Order Osmundales Family Osmundaceae Osmundastrum cinnamomea (Linnaeus) C. Presl Analysis The two surveying strategies – a four-month long terrestrial arthropod survey, followed by a concentrated bioblitz targeting a variety of taxa – resulted in 25,287 and 3,502 specimens barcoded, respectively, out of a total of 32,645 specimens collected. Observations of an additional 125 species and two higher taxa (for which no voucher specimen was kept) were recorded at the bioblitz (Suppl. material 7). Altogether the surveys covered 14 phyla, 29 classes, 117 orders, and 531 families of animals, plants, fungi and lichens. This comprised 3,986 BINs of animals, 1,193 of which are identified to species (Suppl. materials 4, 8, 9). The most diverse groups were Ichneumonidae, Chironomidae, and Cecidomyiidae with 188, 365, and 584 BINs respectively. In terms of abundance, the three groups Sciaridae, Cecidomyiidae, and Chironomidae had the largest number of specimens with 1,528, 2,477, and 9,636 specimens. The most abundant BINs were BOLD:ACC0651 (Thripidae: Taeniothrips inconsequens), BOLD:AAD5253 (Chironomidae: Thienemanniella xena), and BOLD:AAP5920 (Chironomidae: Cricotopus triannulatus) with 349, 636, and 1619 specimens collected. For these three BINs, and many similarly abundant BINs, the presence of closely allied and morphologically similar taxa makes oversampling of these exceptionally common species unavoidable. Combining all existing data results in a final tally of 3,348 species (Table 1), 1,102 of them new records for the reserve (Suppl. material 10). An incidence-based rarefaction analysis (Fig. 2 Suppl. material 11) computed in EstimateS (Colwell and Elsensohn 2014) approximates that 6,744 BINs of invertebrate animals are present at the reserve, indicating that our inventory is at most 30% complete. 1,918 BINs were singletons (i.e. represented by one specimen); the high proportion of singletons (48%) is another indication that the species inventory is far from complete (Magurran 2004). For comparison, the Great Smoky Mountains National Park (North Carolina and Tennessee, United States) established an AT BI effort in 1998 and have tallied 18,545 of the estimated 100,000 species present in the park. In only a few months, our survey found 20% of the Great Smoky Mountains total from 17 years of effort. The Great Smoky Mountains National Park has recognized the contribution DNA barcoding can play in their ATBI effort and have included this method in their survey (e.g., Scholtens and Wagner 2007, Zhou et al. 2011). 166 Telfer A et al. Discussion The present study has conducted an expansive biotic survey, released the data in several public biodiversity data repositories, and published the unique process and findings – all within a relatively modest timeframe. This model for rapid generation and dissemination of critical biodiversity data could be followed to conduct regional assessments of biodiversity status and change, and potentially aid in evaluating progress towards the Aichi Targets of the Strategic Plan for Biodiversity 2011–2020. To fully appreciate this approach, a closer look at four elements is presented: highlights of the biotic inventory, the multiple levels of acceleration, future improvements to the resource, and the utility of the resource going forward. Table 1. Summary of species inventory for rare Charitable Research Reserve in Cambridge, Ontario, Canada, following the present study. Major Group Common Name No. on previous No. on 2015 New New inventory surveys Species Total Annelida Earthworms 0 2 2 2 Arthropoda:Arachnida Spiders and others 0 198 198 198 Arthropoda:Crustacea Crustaceans 0 7 7 7 Arthropoda:Entognatha Collembola 0 9 9 9 Arthropoda:Insecta Insects 832 895 778 1,610 Millipedes, 0 6 6 6 Arthropoda:Myriapoda Centipedes Chordata:Actinopterygii Fishes 28 14 3 31 Chordata:Amphibia Amphibians 13 2 0 13 Chordata:Aves Birds 231 87 0 231 Chordata:Mammalia Mammals 37 6 1 38 Chordata:Reptilia Reptiles 10 0 0 10 Fungi Fungus, Lichens 191 84 60 251 Mollusca Snails, Clams 0 18 18 18 Plantae Plants, Mosses, 901 103 20 921 Protozoans 3 3 0 3 Total Species 2,246 1,433 1,102 3,348 Liverworts Protozoa Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... 167 Figure 2. Accumulation curves for singleton and total observed BINs for the 2015 survey of rare Charitable Research Reserve. Resource Highlights The biotic inventory of rare Charitable Research Reserve performed between May and August 2015 was noteworthy for a number of reasons. The taxonomic scope of the surveys, which covered fourteen phyla over several kingdoms, was only made possible by the integration of DNA barcoding and by assembling a diverse group of experts. Taxa that appeared under-represented on the prior species inventory were targeted where possible, and in many cases, increased significantly. Spiders (Araneae) for instance were completely absent from the prior inventory. Using the expertise of single specialist, focused collecting efforts, and a comprehensive barcode reference library for Canadian spiders (Blagoev et al. 2015) our survey resulted in the addition of 181 species (12.3% of Canadian fauna), two of which were newly recorded for BOLD (Grammonota inornata, Philodromus praelustris) and three representing new provincial records (Cicurina pallida, Neoantistea gosiuta, Xysticus winnipegensis) (Suppl. material 12). Likewise, only 21 lichen species were reported from rare previously, but 53 were collected in a single day at the reserve, increasing the species list to 66. Not all the significant increases were in understudied taxa however: 18 snail and slug species (Mollusca) were added, all new to the reserve; 14 species of fishes were observed, adding three to the previous list of 28; and one mammal (hoary bat, Lasiurus cinereus) of the six observed were added to the list for a total of 37. In terms of BINs, many under-represented taxa did not witness a dramatic increase in named species, but did see large numbers of individual (and unnamed) BINs inventoried; the mites (Acari; 268 BINs) and gall midges (Cecidomyiidae; 584 BINs) are remarkable examples. 168 Telfer A et al. Rapid Creation of the Resource The rapidness of conducting this inventory was due to the acceleration of several steps that comprise the procedure. Firstly, several types of passive traps were employed to acquire large sample sizes for minimal collector effort. For instance, malaise traps often collect 2,000 specimens in a single week with only minutes of servicing time. Secondly, as discussed above, the addition of DNA barcoding streamlines sorting of the material, dividing the specimens into distinct units, minimizing the total number of specimens that require examination. Thirdly, in addition to the initial sorting, barcoding also provides nearly instant taxonomic assignment by querying against reference libraries using BOLD; the resolution of the assignment for queried records depends on the completeness of the reference library. Fourthly, the processing of the bioblitz material and analyses of the DNA barcode data were accelerated at all possible stages to test how quickly a large volume of specimens could proceed through all steps. The generation of data following the bioblitz was impressive: tissue sampling and lysis within 12 hours; DNA extraction and PCR within 24 h; cycle sequencing, cleanup and sequencer loading by 48 h; edited and validated sequences on BOLD by 72 h; taxonomic assignment, data release and manuscript presubmission by 96 h; final manuscript submission to BDJ by 108 h. The laboratory steps are mostly accelerated by automation, while data sharing is greatly facilitated by data release platforms such as BOLD and the Integrated Publishing Toolkit of Canadensys (Robertson et al. 2014), and finally, the manuscript stage is sped up considerably by the online writing tools introduced by Pensoft and other publishers. Refinement of the Resource Many BINs are presently identified only to family, subfamily, or at most, genus level. It's important to note that the inventoried taxa that lack species-level determination, including intractable groups such as mites and midges, will be refined over time. Not only does DNA barcoding, empowered by the BIN system, roughly sort the material to direct and minimize the efforts of taxonomic experts (deWaard et al. 2009), but it also facilitates crowd-sourcing of taxonomic refinement. For example, if two specimens are collected in unrelated projects and locations, but assigned to the same BIN, any taxonomic determination on one specimen will be applicable to the other, and the pertinent data (such as identifier and locality) is shared on the public BIN page. For the specimens of this study, their taxonomy could be honed by the efforts of completely unrelated (but now linked) projects. In addition to this passive approach to refining the taxonomy of these specimens, we will be actively pursuing expert determinations for the unnamed BINs. For many groups, the experts among our coauthors will be able to provide species level resolution in a short period of time (e.g., J.F.-T. specializes in Braconidae, particularly the subfamily Microgastrinae). For other BINs, and over a longer period of time, it will be necessary to solicit determinations from our network of collaborators that specialize on Nearctic taxa. The public release of these data to multiple data repositories should also aid in recruiting active specialists presently outside our network. The vouchered material, all deposited in a single repository (BIOUG), can be quickly assembled and loaned out, along with the Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... 169 associated data and files that may assist in determinations (e.g., tree files and image libraries: Suppl. materials 8, 9). There will however be taxa for which species names will be problematic, due to a lack of active specialists, keys, and/or revisionary work, and where a large proportion of that taxon awaits description. For example, the number of gall midges (Diptera: Cecidomyiidae) inventoried for rare (584) is nearly six times the number of Canadian species described to date (ca. 100, following Gagne and Jaschhof 2014). When a significant number of species and other taxon categories become available, the refined dataset can be updated in the various data repositories and potentially in a second, updated version of the paper in BDJ. Any subsequent versions – each with a separate Digital Object Identifier (DOI) – would be linked to the original paper, allowing for continuing improvement and additions to the species list for the reserve. It is important to emphasize that the taxa awaiting species-level determination still have persistent identifiers as part of the BIN system; each BIN is assigned a BOLD-generated uniform resource identifier (URI) upon its establishment (e.g. BOLD:AAA0001). Until the species binomial is determined or described, this URI can be used in its place. For example, this URI permits assessing and comparing local diversity (Young et al. 2012) despite uncertainty in taxonomic placement. Utility of the Resource While the overarching objective of the study was to develop, assess and demonstrate a model for conducting and disseminating DNA barcode-assisted biotic surveys, a valuable resource was created in the process. The rare Charitable Research Reserve provides a unique urban reserve with the infrastructure necessary to conduct research in its diversity of habitats; a barcode reference library and updated species inventory can now both be added to the infrastructure shared with their researchers and educators. Ecological studies in particular, such as the ongoing prairie community field experiments (e.g., Harvey and MacDougall 2014), could benefit greatly from this resource that allows the investigation of ecological questions previously impossible to address (Joly et al. 2014, Kress et al. 2015). Moreover, these studies and others are increasingly using next generation sequencing (NGS) technologies, where validated reference libraries linked to voucher specimens are of critical importance, since this link is generally lost in NGS studies (Cristescu 2014). This reference library created can also be amalgamated with external barcode records, local inventories, and taxon-specific efforts to create regional and national libraries; this study for instance has enhanced the reference library for animals by providing 468 unique BINs to BOLD. Finally, as mentioned above, it is crucial that we begin to assess biodiversity change and disseminate these data widely -- the resource created here can form the baseline for accessible, repeated assessments, to gauge trends in an important temperate reserve. 170 Telfer A et al. Acknowledgements Financial support was provided by the Ontario Ministry of Research and Innovation and by the government of Canada through Genome Canada and the Ontario Genomics Institute in support of the International Barcode of Life project. Anne McCain Evans and Chris Evans provided support for the BIObus program (www.biobus.ca), whose students conducted much of the field work. A biotic survey and bioblitz of this magnitude could not be completed without the help of many volunteers; we would like to thank the following for their contributions to this study: Reza Zahiri, Carleigh Pope, Cheyanne Richardson, Christine Thompson, Dan Radoslav, Dave Achtymichuk, Erika Kastner, Kim Robichaud, Mike Achtymichuk, Tim Skuse, Lisandra Gal, and Suz Bateson. We would also like to thank our reviewers for their helpful suggestions and contributions to this manuscript: Jeremy Miller, Pavel Stoev, and Torsten Dikow. Author contributions Author contributions: P.D.N.H. and J.R.D. designed the study; J.S., C.N.S. and M.R.Y. oversaw specimen and sequence analyses; A.C.T., J.R.D. and V.L.-B. conducted the analyses. J.M. and E.S. provided specimen images. All authors discussed the results and contributed to specimen collection, identifications, and to the manuscript, which was written by J.R.D., R.D., J.F.-T. References • • • • • • • Adamowicz SJ (2015) International Barcode of Life: Evolution of a global research community. Genome 58: 151‑162. DOI: 10.1139/gen-2015-0094 Blagoev G, deWaard J, Ratnasingham S, deWaard S, Lu L, Robertson J, Telfer A, Hebert PN (2015) Untangling taxonomy: a DNA barcode reference library for Canadian spiders. Molecular Ecology Resources n/a: n/a‑n/a. DOI: 10.1111/1755-0998.12444 Bortolus A (2008) Error Cascades in the Biological Sciences: The Unwanted Consequences of Using Bad Taxonomy in Ecology. AMBIO: A Journal of the Human Environment 37 (2): 114‑118. DOI: 10.1579/0044-7447(2008)37[114:ecitbs]2.0.co;2 Brunke A, Majka C (2010) The adventive genus Xantholinus Dejean (Coleoptera, Staphylinidae, Staphylininae in North America: new records and a synthesis of distributional data. ZooKeys 65: 51‑61. DOI: 10.3897/zookeys.65.574 Check E (2006) Treasure island: pinning down a model ecosystem. Nature 439 (7075): 378‑379. DOI: 10.1038/439378a Colwell R, Elsensohn J (2014) EstimateS turns 20: statistical estimation of species richness and shared species from samples, with non-parametric extrapolation. Ecography 37 (6): 609‑613. DOI: 10.1111/ecog.00814 Cristescu M (2014) From barcoding single individuals to metabarcoding biological communities: towards an integrative approach to the study of global biodiversity. Trends in Ecology & Evolution 29 (10): 566‑571. DOI: 10.1016/j.tree.2014.08.001 Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... • • • • • • • • • • • • • 171 deWaard J, Ivanova N, Hajibabaei M, Hebert PN (2008) Assembling DNA Barcodes. Methods in Molecular Biology. URL: http://dx.doi.org/10.1007/978-1-59745-548-0_15 DOI: 10.1007/978-1-59745-548-0_15 deWaard J, Landry J, Schmidt BC, Derhousoff J, McLean J, Humble L (2009) In the dark in a large urban park: DNA barcodes illuminate cryptic and introduced moth species. Biodiversity and Conservation 18 (14): 3825‑3839. DOI: 10.1007/ s10531-009-9682-7 Díaz S, Demissew S, Joly C, Lonsdale WM, Larigauderie A (2015) A Rosetta Stone for Nature’s Benefits to People. PLOS Biology 13 (1): e1002040. DOI: 10.1371/ journal.pbio.1002040 Fazekas A, Kuzmina M, Newmaster S, Hollingsworth P (2012) DNA Barcoding Methods for Land Plants. Methods in Molecular Biology™. URL: http:// dx.doi.org/10.1007/978-1-61779-591-6_11 DOI: 10.1007/978-1-61779-591-6_11 Gagne RJ, Jaschhof M (2014) A Catalogue of the Cecidomyiidae (Diptera) of the world. 3rd edition. Digital version 2. Harvey E, MacDougall A (2014) Trophic island biogeography drives spatial divergence of community establishment. Ecology 95 (10): 2870‑2878. DOI: 10.1890/13-1683.1 Hebert PDN, Cywinska A, Ball SL, deWaard JR (2003) Biological identifications through DNA barcodes. Proceedings of the Royal Society B: Biological Sciences 270 (1512): 313‑321. DOI: 10.1098/rspb.2002.2218 Henry T, Bainard J, Newmaster S, Schwarzacher T (2014) Genome size evolution in Ontario ferns (Polypodiidae): evolutionary correlations with cell size, spore size, and habitat type and an absence of genome downsizing. Genome 57 (10): 555‑566. DOI: 1 0.1139/gen-2014-0090 Hollingsworth PM (2011) Refining the DNA barcode for land plants. Proceedings of the National Academy of Sciences 108 (49): 19451‑19452. DOI: 10.1073/pnas.1116812108 Hollingsworth PM, Forrest LL, Spouge JL, Hajibabaei M, Ratnasingham S, van der Bank M, Chase MW, Cowan RS, Erickson DL, Fazekas AJ, Graham SW, James KE, Kim K-, Kress WJ, Schneider H, AlphenStahl Jv, Barrett SC, Berg C, Bogarin D, Burgess KS, Cameron KM, Carine M, Chacon J, Clark A, Clarkson JJ, Conrad F, Devey DS, Ford CS, Hedderson TA, Hollingsworth ML, Husband BC, Kelly LJ, Kesanakurti PR, Kim JS, Kim Y-, Lahaye R, Lee H-, Long DG, Madrinan S, Maurin O, Meusnier I, Newmaster SG, Park C-, Percy DM, Petersen G, Richardson JE, Salazar GA, Savolainen V, Seberg O, Wilkinson MJ, Yi D-, Little DP (2009) A DNA barcode for land plants. Proceedings of the National Academy of Sciences 106 (31): 12794‑12797. DOI: 10.1073/pnas.0905845106 Ivanova N, Grainger C (2006) Pre-made frozen PCR and sequencing plates. http:// www.dnabarcoding.ca/CCDB_DOCS/ CCDB_Advances_Methods_Release_No4_Dec1st_2006.pdf. Accession date: 2015 8 17. Ivanova N, deWaard J, Hebert PN (2006) An inexpensive, automation-friendly protocol for recovering high-quality DNA. Molecular Ecology Notes 6 (4): 998‑1002. DOI: 10.111 1/j.1471-8286.2006.01428.x Ivanova N, Fazekas A, Hebert PN (2008) Semi-automated, Membrane-Based Protocol for DNA Isolation from Plants. Plant Molecular Biology Reporter 26 (3): 186‑198. DOI: 1 0.1007/s11105-008-0029-4 172 • • • • • • • • • • • • Telfer A et al. Ivanova N, Kuzmina M, Fazekas A (2011) CCDB Protocols. Glass Fiber Plate DNA Extraction Protocol For Plants, Fungi, Echinoderms and Mollusks, Manual Protocol Employing Centrifugation. http://www.ccdb.ca/CCDB_DOCS/CCDB_DNA_ExtractionPlants.pdf. Accession date: 2015 8 17. Janzen D (1993) Taxonomy: Universal and Essential Infrastructure for Development and Management of Tropical Wildland Biodiversity. In: Sandlund OT, Schei PJ (Eds) Proc. Norway UNEP Expert Conference on Biodiversity, Trondheim. Janzen D (2004) Setting up tropical biodiversity for conservation through non-damaging use: participation by parataxonomists. Journal of Applied Ecology 41 (1): 181‑187. DOI: 10.1111/j.1365-2664.2004.00879.x Janzen D, Hallwachs W, Blandin P, Burns J, Cadiou J, Chacon I, Dapkey T, Deans A, Epstein M, Espinoza B, Franclemont J, Haber W, Hajibabaei M, Hall JW, Hebert PN, Gauld I, Harvey D, Hausmann A, Kitching I, Lafontaine D, Landry J, Lemaire C, Miller J, Miller J, Miller L, Miller SE, Montero J, Munroe E, Green SR, Ratnasingham S, Rawlins J, Robbins R, Rodriguez J, Rougerie R, Sharkey M, Smith MA, Solis MA, Sullivan JB, Thiaucourt P, Wahl D, Weller S, Whitfield J, Willmott K, Wood DM, Woodley N, Wilson J (2009) Integration of DNA barcoding into an ongoing inventory of complex tropical biodiversity. Molecular Ecology Resources 9: 1‑26. DOI: 10.1111/ j.1755-0998.2009.02628.x Janzen DH, Hallwachs W (1994) All Taxa Biodiversity Inventory (ATBI) of Terrestrial Systems: A Generic Protocol for Preparing Wildland Biodiversity for Non-Damaging Use. Report of a National Science Foundation Workshop, 16–18 April 1993, Philadelphia, Pennsylvania. On-line at www.all-species.org/content/reference/ ATBI_Fin_Rep_8feb94_.pdf. Joly S, Davies TJ, Archambault A, Bruneau A, Derry A, Kembel S, Peres-Neto P, Vamosi J, Wheeler T (2014) Ecology in the age of DNA barcoding: the resource, the promise and the challenges ahead. Molecular Ecology Resources 14 (2): 221‑232. DOI: 10.1111/1755-0998.12173 Kress WJ, García-Robledo C, Uriarte M, Erickson D (2015) DNA barcodes for ecology, evolution, and conservation. Trends in Ecology & Evolution 30 (1): 25‑35. DOI: 10.1016/ j.tree.2014.10.008 Kuzmina M, Ivanova N (2011) PCR Amplification for Plants and Fungi . http:// www.ccdb.ca/CCDB_DOCS/CCDB_Amplification-Plants.pdf. Accession date: 2015 8 17. Lawton JH, Bignell DE, Bolton B, Bloemers GF, Eggleton P, Hammond PM, Hodda M, Holt RD, Larsen TB, Mawdsley NA, Stork NE, Srivastava DS, Watt AD (1998) Biodiversity inventories, indicator taxa and effects of habitat modification in tropical forest. Nature 391 (6662): 72‑76. DOI: 10.1038/34166 Lundmark C (2003) BioBlitz: Getting into Backyard Biodiversity. BioScience 53 (4): 329. DOI: 10.1641/0006-3568(2003)053[0329:bgibb]2.0.co;2 Magurran A (2004) Measuring biological diversity. Blackwell Science, 264 pp. Merckx VFT, Hendriks K, Beentjes K, Mennes C, Becking L, Peijnenburg K, Afendy A, Arumugam N, Boer Hd, Biun A, Buang M, Chen P, Chung AC, Dow R, Feijen FA, Feijen H, Soest CF, Geml J, Geurts R, Gravendeel B, Hovenkamp P, Imbun P, Ipor I, Janssens S, Jocqué M, Kappes H, Khoo E, Koomen P, Lens F, Majapun R, Morgado L, Neupane S, Nieser N, Pereira J, Rahman H, Sabran S, Sawang A, Schwallier R, Shim P, Smit H, Sol N, Spait M, Stech M, Stokvis F, Sugau J, Suleiman M, Sumail S, Thomas Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... • • • • • • • • • • • • • 173 D, Tol Jv, Tuh FY, Yahya B, Nais J, Repin R, Lakim M, Schilthuizen M (2015) Evolution of endemism on a young tropical mountain. Nature 000: 1‑3. DOI: 10.1038/nature14949 Mosquin T, McAllister DE, Whiting P (1995) Canada's biodiversity: the variety of life, its status, economic benefits, conservation costs and unmet needs /. Canadian Museum of Nature, 293 pp. URL: http://dx.doi.org/10.5962/bhl.title.101447 DOI: 10.5962/ bhl.title.101447 Novotny V, Miller SE, Hulcr J, Drew RA, Basset Y, Janda M, Setliff GP, Darrow K, Stewart AJ, Auga J, Isua B, Molem K, Manumbor M, Tamtiai E, Mogia M, Weiblen GD (2007) Low beta diversity of herbivorous insects in tropical forests. Nature 448: 692‑695. Packer L, Gibbs J, Sheffield C, Hanner R (2009) DNA barcoding and the mediocrity of morphology. Molecular Ecology Resources 9: 42‑50. DOI: 10.1111/ j.1755-0998.2009.02631.x Pimm SL, Russell GJ, Gittleman JL, Brooks TM (1995) The Future of Biodiversity. Science 269 (5222): 347‑350. DOI: 10.1126/science.269.5222.347 Pimm SL, Jenkins CN, Abell R, Brooks TM, Gittleman JL, Joppa LN, Raven PH, Roberts CM, Sexton JO (2014) The biodiversity of species and their rates of extinction, distribution, and protection. Science 344 (6187): 1246752‑1246752. DOI: 10.1126/ science.1246752 Porco D, Rougerie R, Deharveng L, Hebert PN (2010) Coupling non-destructive DNA extraction and voucher retrieval for small soft-bodied Arthropods in a high-throughput context: the example of Collembola . Molecular Ecology Resources 10 (6): 942‑945. DOI: 10.1111/j.1755-0998.2010.2839.x Ratnasingham S, Hebert PN (2007) BOLD: The Barcode of Life Data System (http:// www.barcodinglife.org). Molecular Ecology Notes 7 (3): 355‑364. DOI: 10.1111/ j.1471-8286.2007.01678.x Ratnasingham S, Hebert PN (2013) A DNA-Based Registry for All Animal Species: The Barcode Index Number (BIN) System. PLoS ONE 8 (7): e66213. DOI: 10.1371/ journal.pone.0066213 Robertson T, Döring M, Guralnick R, Bloom D, Wieczorek J, Braak K, Otegui J, Russell L, Desmet P (2014) The GBIF Integrated Publishing Toolkit: Facilitating the Efficient Publishing of Biodiversity Data on the Internet. PLoS ONE 9 (8): e102623. DOI: 10.137 1/journal.pone.0102623 Schilthuizen M, Vairappan C, Slade E, Mann D, Miller J (2015) Specimens as primary data: museums and ‘open science’. Trends in Ecology & Evolution 30 (5): 237‑238. DOI: 10.1016/j.tree.2015.03.002 Schoch C, Seifert K, Huhndorf S, Robert V, Spouge J, Levesque CA, Chen W, Consortium FB (2012) Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi . Proceedings of the National Academy of Sciences of the United Sates of America 109 (16): 6241‑6246. DOI: 10.1073/ pnas.1117018109 Scholtens B, Wagner D (2007) Lepidoptera of Great Smoky Mountains National Park: Methods and Results of the Inventory. Southeastern Naturalist 6: 193‑206. DOI: 10.165 6/1528-7092(2007)6[193:logsmn]2.0.co;2 Sharkey M (2001) The All Taxa Biological Inventory of the Great Smoky Mountains National Park. The Florida Entomologist 84 (4): 556. DOI: 10.2307/3496388 174 • • • • • • Telfer A et al. Simberloff D (1996) Lawton, J. H. and May, R. M. (Eds.). Extinction Rates. 1995. Oxford University Press, Oxford. xii + 233 pp. ISBN: 0-19-854829. X. Price: f17.95. Journal of Evolutionary Biology 9 (1): 124‑126. DOI: 10.1046/j.1420-9101.1996.t01-1-9010124.x Wirta HK, Vesterinen EJ, Hambäck PA, Weingartner E, Rasmussen C, Reneerkens J, Schmidt NM, Gilg O, Roslin T (2015) Exposing the structure of an Arctic food web. Ecology and Evolution X: In press. Woodcock T, Pekkola L, Dawson C, Gadallah F, Kevan P (2014) Development of a Pollination Service Measurement (PSM) method using potted plant phytometry. Environmental Monitoring and Assessment 186 (8): 5041‑5057. DOI: 10.1007/ s10661-014-3758-x Young M, Behan-Pelletier V, Hebert PN (2012) Revealing the Hyperdiverse Mite Fauna of Subarctic Canada through DNA Barcoding. PLoS ONE 7 (11): e48755. DOI: 10.137 1/journal.pone.0048755 Zhou X, Adamowicz SJ, Jacobus LM, DeWalt RE, Hebert PD (2009) Towards a comprehensive barcode library for arctic life - Ephemeroptera, Plecoptera, and Trichoptera of Churchill, Manitoba, Canada. Frontiers in Zoology 6 (1): 30. DOI: 10.118 6/1742-9994-6-30 Zhou X, Robinson J, Geraci C, Parker C, Flint O, Etnier D, Ruiter D, DeWalt RE, Jacobus L, Hebert PN (2011) Accelerated construction of a regional DNA-barcode reference library: caddisflies (Trichoptera) in the Great Smoky Mountains National Park. Journal of the North American Benthological Society 30 (1): 131‑162. DOI: 10.1899/10010.1 Supplementary materials Suppl. material 1: Species inventory of rare prior to May 2015 Authors: Telfer et al. Data type: checklist Brief description: Species inventory for rare Charitable Research Reserve in Cambridge, Canada as of May 2015, prior to the present study. Filename: Suppl materials 1 - Existing Inventory.xlsx - Download file (156.97 kb) Suppl. material 2: Bat protocols Authors: Telfer et al. Data type: protocols Brief description: Protocols for the bat component of rare BioBlitz Filename: Suppl materials 2 - Bat protocols.pdf - Download file (362.98 kb) Suppl. material 3: Lysis, primer and marker details Authors: Telfer et al. Data type: laboratory Brief description: Details for laboratory work. Filename: Suppl materials 3 - Lab Details.xlsx - Download file (10.47 kb) Biodiversity inventories in high gear: DNA barcoding facilitates a rapid ... 175 Suppl. material 4: Summary data for 2015 inventory Authors: Telfer et al. Data type: summary Brief description: Summary data for the 28,789 specimens collected for the 2015 inventory, including BIN assignments and locality Filename: Suppl materials 4 - Summary Data.xlsx - Download file (2.18 MB) Suppl. material 5: Darwin core archive Authors: Telfer et al. Data type: occurrences Brief description: Darwin core archive of 2015 rare species inventory Filename: Suppl materials 5 - Darwin Core Archive.zip - Download file (1.05 MB) Suppl. material 6: Contributors list Authors: Telfer et al. Data type: authors Brief description: A list of all contributors who took part in the collection and identification of specimens collected as part of the 2015 inventory. Filename: Suppl materials 6 - Contributors.xls - Download file (43.00 kb) Suppl. material 7: Human observations during rare BioBlitz Authors: Telfer et al. Data type: occurrences Brief description: Human observations during rare BioBlitz, of mostly plants, birds, etc. Filename: Suppl materials 7 - Species sightings.xlsx - Download file (23.87 kb) Suppl. material 8: BIN representative tree Authors: Telfer et al. Data type: tree Brief description: A neighbour-joining tree constructed from a single representative of each distinct BIN collected in rare, along with a single representative of clusters without BINs. Filename: Suppl materials 8 - COI Tree.pdf - Download file (242.06 kb) Suppl. material 9: BIN image library Authors: Telfer et al. Data type: images Brief description: A collection of representative images of each BIN collected from the rare Charitable Research Reserve. BINs are listed in the same order as the tree. Specimens without BINs are not included. Filename: Suppl materials 9 - Tree Images.pdf - Download file (163.93 MB) 176 Telfer A et al. Suppl. material 10: Final combined inventory for rare Charitable Research Reserve Authors: Telfer et al. Data type: checklist Brief description: The final combined inventory for rare Charitable Research Reserve as of August 2015; including previous checklist and all 2015 species added. Filename: Suppl materials 10 - Final Inventory.xlsx - Download file (328.53 kb) Suppl. material 11: Raw data for accumulation curve Authors: Telfer et al. Data type: occurences Brief description: Lot and BIN data was downloaded for each specimen that received a BIN from BOLD Systems. It was formatted for input into EstimateS (Version 9.1.0) for creation of an accumulation curve. Filename: Suppl materials 11 - Accumulation Curve data.xlsx - Download file (477.00 kb) Suppl. material 12: Sampling and new record images Authors: Telfer et al. Data type: images Brief description: Images of sampling sites, sampling techniques, specimen processing, and three new provincial spider reccords. Filename: Suppl materials 12 - Sampling and Specimen Images.pdf - Download file (1.16 MB)