Rev. Fr. H. Santapau, S. J. Theodore Cooke, Norman Ernest Borlaug Jagadish Chandra Bose In the Memories of Great Botanist Har Gobind Khorana Birbal Sahni M. J. Thirumalachar Prof . G. V. Joshi Prof. V.N. Naik, P. Maheshwari Men love to Wonder, and that is the Seed of Science. ……..Ralph Waldo Emerson NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY JANUARY 18th - 19th, 2013 ABSTRACT BOOK Organized by DEPARTMENT OF BOTANY YASHAVANTRAO CHAVAN INSTITUTE OF SCIENCE, SATARA (MS) 415001 Reaccredited by NAAC with A Grade (CGPA 3.37) College with Potential for Excellence Sponsored by University Grants Commission, WRO, Pune. Website: www.erayat.org/ycis Email: ycis_satara@dataone.in DISCLAIMER:  The authors are responsible for the contents of abstracts/ synopsis of the lecture.  Abstracts are arranged alphabetically by considering first author’s surname.  Abstracts are printed as per mail provided by authors. PUBLISHED BY: Dr. A. B. Pawar, I/C Principal , Yashavantrao Chavan Institute of Science, Satara. Dist. Satara, Maharashtra, India-415001. Mob-08380063895 Ph-02162-230302 PRINTED AT: Shree Gajanan Press, Satara. Chief Patron : Hon. Shri Sharad Pawar, Union Minister of Agriculture & Food Processing Industries, President, Rayat Shikshan Sanstha. Satara. Patrons: Hon. Adv. Shri Raosaheb Shinde, Chairman, Rayat Shikshan Sanstha. Satara. Hon.Dr. Anil Patil, Vice-Chairman, Rayat Shikshan Sanstha, Satara. Hon. Dr. N. D. Patil, Ex-Chairman, Rayat Shikshan Sanstha, Satara. Advisory Committee Prin.Dr. A. S. Burungale, Secretary, Rayat Shikshan Sanstha, Satara. Hon. Dr. N. S. Gaikwad, Joint Secretary, Rayat Shikshan Sanstha, Satara. Hon. Prof. N. J. Pawar, Vice Chancellor, SUK. Hon. Dr. A.S. Bhoite, Pro Vice- Chancellor, SUK. Prin. C. J. Khilare, Dean of Science faculty, SUK. Dr. A. B. Pawar. I/C Prin. Yashavantrao Chavan Institute of Science, Satara. Mr. G. Srinivas, Joint Secretary, UGC, WRO, Pune. Prof. S. R. Yadav, Department of Botany, SUK Prof. S. S. Kamble, HOD, Dept. of Botany, Shivaji University Kolhapur. Prof. K. N. Dhumal, University of Pune Prin. K. N. Jagdale , Sharadchandra Pawar College of A. S. and C. Lonand . Mr. V. G. Phadtare, Chairman, Kavistu Group of Industries, Satara. Organizing Committee Prin. Dr. A. S. Burungale (President) Dr. (Mrs.) V. C. Karande Mr. S. V. Thite I/C Pricipal Dr. A. B. Pawar (Convenor) Mr. S. S. Gaikwad Dr. J. J. Chavan Dr. N. A. Ghanawat (Coordinator) Mr. H. L. Shinde Mr. Y. A. Ghule Dr. B. A. Kore (Secretary) Mr. S. N. Jadhav Dr. V. T. Aparadh Dr. R. B. Thoke (Treasurer) Mr. N. B. Mane Dr. S. S. Sagar Mrs. R. A. Shinde Mr. D. A. Kadam Mr. A. S. Kadam Mr. P. R. Patil NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chav Chavan an Institute of Science, Satara 18th & 19th January, 2013 BLACK MILDEWS V.B. Hosagoudar Jawaharlal Nehru Tropical Botanic Garden and Research Institute, Palode-587 Palode 587 116, Thiruvananthapuram, Kerala “Black Mildews” are the black colony forming ectophytic obligate biotrophs producing dark or brown mycelium, fruiting bodies, spores, etc. These fungi maintain harmony between the host and the parasites but very few produce pathogenic effect of the host plants. Since these fungi are host specific, they they cannot be cultured. Similarly, “sooty moulds” are another group of fungi, always associated with the sugary secretion producing insects or plant glands. There is no specificity for these fungi, cover both living and non-living non living parts of the plants includ including ing nearby plants and the colonies can be easily removed by moving the fingers on them. The commonly used name “black mildew” comprises large number of fungi belonging to the groups, e.g. Meliolales, Asterinales, Englerulaceae, Schiffnerulaceae, Meliolin Meliolinaceae, aceae, Hyphomycetes, etc. Study of these fungi in situ merits much and hence, a nail polish technique is employed. The order Meliolales characterized by its brown mycelium, two celled appressoria, celled globose or flattenedflattened-globose globose perithecia, evanescent unitunicate unitunicate or prototunicate asci, 11--4-celled ascospores brown ascospores. This order comprises: ArmatellaceaeArmatellaceae having no phialides, 11-2--ascospores Basavamyces,, having with two genera: Armatella-having Armatella having the centrally septate ascospores and Basavamyces distally two septate ascospores. The The family Meliolaceae comprises the genera Amazonia (Perithecia flattenedflattened-globose), globose), Appendiculella (having only perithecial appendages), Asteridiella (lacks perithecial and mycelia setae), Ectendomeliola (having both ecto and endo type of mycelium), Irenopsis (posessing perithecial setae), Meliola (having mycelial setae) and Prataprajella (having both perithecial appendages and repent setae). Identification procedure description tion to digital involves: identity of the host plant and its family, conversion of the fungus descrip formula and matching the line drawings with the icon of the probable species. The second largest group of black mildew is Asterinales. This order differs from the other Microthyriaceae yriaceae in having similar groups having thyriothecia, namely, Parmulariaceae and Microth thyriothecia dehisce or split open and having external mycelium. The order comprises PLENARY LECTURE NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 Asteriaceae and Lembosiaceae. In Asterinaceae, orbicular thyriothecia dehisce stellately at the centre, while in Lembosiaceae oval or elongated thyriothecia dehisce longitudinally. The family Asterinaceae comprises Asterina (appressoria lateral), Asterolibertia (appressoria intercalary), Ishwaramyces (appressoria in clusters at the septal region), Meliolaster (ascospores two septate and elongated at one end), Prillieuxina (devoid of appressoria), Symphaster (appressoria produced only around the stomatal guard cells), Trichasterina (mycelial or perithecial or both present) and Vishnumyces (ascospores with one pinch-off cell). The family Lembosiaceae comprises Cirsosia (having intercalary appressoria), Echidnodella (appressoria and hypostroma absent), Echidnodes (appressoria absent and hypostroma present), Eupelte (1-3-septate conidia present), Lembosia (lateral appressoria present) and Maheshwaramyces (conidia one to many septate). Based on the generic identity, corresponding species of that genus under the family of the corresponding host plant is to be matched to confirm the fungus to be identified up to species level. The family Schiffnerulaceae comprises the genus Schiffnerula and its synanamorphs, characterized by having brown, appressoriate mycelium with unicellular appressoria. Ascomata arise from the short lateral branches, initially with radiating cells but the cells dissolve when the ascomata start resuming globose appearance. Asci few, bitunicate, broadly ellipsoid to globose, sessile, octosporous, exposed after deliqusing the ascomatal wall; ascospores brown, 1-septate, constricted at the septum. This genus includes four synanamorphs: Questieriella (3-septate fusiform conidia), Mitteriella (ellipsoidal to limoniform 0-4-septate conidia), Digitosarcinella (cheiroid, with 4-5 closely appressed arms, up to 7-septate conidia-not known in India) and Sarcinella (sarciniform, dark brown to reddish brown conidia). Ascigerous state belongs to the genus Schiffnerula. Including synanamorphs, this genus represents almost 100 species in India. Englerulaceae comprises the genera: Alina (non-appressoriate mycelium and having conidia), Balladyna (mycelial setae present), Balladynocallia (Mycelial setae absent), Balladynopsis (perithecial setae present), Dysrhynchis (mycelium non-appressoriate and conidia absent). PLENARY LECTURE NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 Another interesting genus Meliolina, belongs to the genus Meliolinaceae, comprises about 36 species and of which about 5 are in India. It infects the members of Myrtaceae and Melastomataceae in India. Infection mostly restricted to the lower surface of the leaves, which are woolly and very thick, can be identified easily. Interestingly, all these fungi are hyperparasitized by other fungi, which are once again fungus specific, form woolly and mask the identity of the host fungus. In addition to these, the members of Parmulariaceae, Microthyriaceae and several other dematiaceous fungi form black colonies and we shall reserve this study for the future researchers. PLENARY LECTURE NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chav Chavan an Institute of Science, Satara 18th & 19th January, 2013 Phytoremediation technologies as an advanced tool for the removal of dyes from textile effluent Prof. S. P. Govindwar Department of Biochemistry, Shivaji University, Kolhapur-416004 Kolhapur 416004 spg_biochem@unishivaji.ac.in Phytoremediation involves the use of plants and rhizospheric organisms for the removal of pollutants which is an emerging technology for the cleanup of contaminated sites. The removal of textile dyes mediated by plants has been one of the most neglected ar areas eas of phytoremediation research. Dyes, which are primary constituents of the waste from the textile industry effluents, constitute a group of recalcitrant compounds, many of which are known to have toxic and carcinogenic effects. The study of the mechanisms mechanisms adopted by the plants in the removal of textile dyes and the future scope for research in this area which will help in broadening the horizons of phytoremediation technologies. Plant species many a times referred to as ‘green livers’, are known to possess possess a wide range of detoxifying and biotransforming enzymes some of which may also be secreted extracellularly in the rhizosphere and can bring about the transformation of organic pollutants such as textile dyes. The use of in vitro plants for phytoremediation studies can help to explore the enzymatic status and the products of phytoremediation metabolism of the dye, thus providing a new dimension to phytoremediation studies. Various an plants alone or in combination with other plants or microorganisms growing on its exudates ccan combine the advantages of both plant and microbial systems for enhanced dye degradation in laboratory scale phytoreactor. Biotechnological approaches involving the development of hairy roots and suspension cultures may find good utility in phytoremediation phytoremediation studies. The ultimate aim of phytoremediation involves applying these well studied plant systems at the contaminated on--site site treatment of sites which may constitute the development of constructed wetlands for on textile industrial effluents. PLENARY LECTURE NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 NANOBIOTECHNOLOGY: AN APPROACH TOWARDS GREEN REVOLUTION A. R. Nalwade Plant Tissue Culture Research Laboratory, Annasaheb Awate College, Manchar. Dist. – Pune (M.S.), INDIA– 410503 abasahebnalwade@gmail.com The origin of nanoscience can be traced to clay mineralogy and crystallography (Lower et al., 2001). The prefix ‘nano’ a Greek word signifying ‘dwarf’. This word was used for the first time by Richard Feyman (1959). Nanotechnology is the production, manipulation and use of materials ranging in size from 1 to 100 nm. Increased surface area allows chemical reactions to go faster. It can be defined as “ability to work at the atomic, molecular and sub-molecular levels in order to create and use material structures, devices and systems with new properties and functions. The properties of matter change at the nanoscale e.g. colour, chemical reactivity. Methods of synthesizing nanoparticles: Nanoparticles can be synthesized by various routes viz. Chemical reduction, Photo- chemical reduction, Laser irradiation technique, Thermal decomposition, Electrochemical, Sonochemical, Microwave assisted, Green nanotechnology. Green synthesis is the synthesis of nanoparticles by using living organisms or their products. The advantages of green nanotechnology are: Environmentally friendly manufacturing process, cost effective, easily scaled up for large scale synthesis, no need of high pressure, energy, temperature, toxic chemicals etc. (Mohanpuria et al., 2008; Sharma et al., 2009; Gade et al., 2010). Applications of nanoparticles and nano-devices in agriculture: Among the latest lines of technological innovations, nanotechnology occupies a prominent position in transforming agriculture and food production. The development of nanodevices and nanomaterials could open up novel applications in plant biotechnology and agriculture (Nair et al., 2010) PLENARY LECTURE NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 Crop protection: Nano-capsulated agrochemicals have designed in such a way that they possess all necessary properties, time controlled release in response to certain stimuli, enhanced targeted delivery and less toxicity with and easy mode of delivery (Green et al., 2007; Wang et al., 2007). The control of parasitic weeds by nano-capsulated herbicide can reduce toxicity of herbicide on crop plants (Perez-de-Luque and Diego, 2009). Surface modified hydrophobic nanosilica can control a range of agricultural insect pests. These nano-biopesticides are safe for plants and cause less environmental pollution in comparison to conventional chemical pesticides. Nanoparticles and fertilizers: The controlled-release fertilizers cemented by nanomaterials were safe for wheat seed germination, emergence and growth of seedlings (Liu et al., 2006; Zang et al., 2006). Impact of different nanoparticles on plants: Carbon nanomaterials found increased applications in the field of agriculture. Many studies have reported that carbon nanomaterials could stimulate seed germination and seedling growth (Khodaskovaya et al., 2009; Canas et al., 2008). Carbon nanotubes (CNT) showed ability to carry single stranded DNA into intact plant cells (Liu et al., 2009).TiO2 nanoparticles improved light absorbance and promoted the activity of ribulose 1,5bisphosphatecarboxylae thus, accelerated spinach growth (Zheng et al., 2005; Mingyu et al., 2008). They also enhance nitrogen metabolism and promoted the activity of antioxidant enzymes like SOD, catalase, ascorbic oxidase and guaiacol peroxidase (Lei et al., 2008; Bryner et al., 2006).ZnO nanoparticles increased permeability and creation of new holes in the bacterial cell wall.Copper nanoparticles have variable effects on the growth of different plants (Shah and Belozerova, 2009).Silver nanoparticles have several antimicrobial functions to control various plant pathogens (Jo et al., 2009). They are highly effective against bacterial and spore producing fungal phytopathogens (Jo and Kim, 2009). PLENARY LECTURE NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 Nanomaterials in gene delivery: Nanomaterials and nanoparticles have an important role in gene delivery a process similar with microinjection method (Shegura and Shea, 2001). Nanoparticles biomaterial was designed in such way that it binds and transport genes across the cell wall of plant cells by inducing instant pores in cell wall, cell membrane and nuclear membrane. It helps in nanoparticles mediated DNA transfer that might be more successful in regenerative calli and soft tissue (Jun et al., 2008). Smart Delivery System: Smart Delivery System in agriculture should possess combination of time controlled, specifically targeted, highly controlled, remotely regulated and multifunctional characteristics to avoid biological barriers for successful targeting. Technologies such as encapsulation and controlled release methods have revolutionized the use of pesticides and herbicides. Many companies prepared formulations which contain nanoparticles within the range 100-250 nm size, which are able to dissolve in water more efficiently than existing one. Other companies employ suspensions of nanoscale, which can be either water or oil based and contain uniform suspensions of pesticidal or herbicidal nanoparticles. These can be easily incorporated in various media such as gels, creams and liquids and have multiple applications for preventive measures, preservation of harvested products. In other areas, scientists are working on various technologies to make fertilizer and pesticide delivery systems which can respond to environmental changes. The ultimate aim is to tailor these products in such a way that they will release their cargo in a controlled manner (slowly or quickly) in response to different signals e.g. heat, moisture etc. Precision Farming: It has been a long-desired goal to maximize output (crop yield) while minimizing input (fertilizers, pesticides, herbicides etc.) through monitoring environmental variables and applying targeted action. Precision farming makes use of computers, global satellite positioning system and remote sensing devices to measure highly localized environmental conditions, thus PLENARY LECTURE NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 determining whether crops are growing at maximum efficiency or precisely identifying the nature and location of problems. Precision farming with the help of smart sensors, will allow enhanced productivity in agriculture by providing accurate information, thus helping farmers to take better decisions. Nanotechnology in food industry: The definition of nanofood is that nanotechnology techniques or tools are used during cultivation, production, processing or packaging of food. Nanotechnologists are more optimistic about the potential to change the existing system of food processing and to ensure the safety of food products, creating a healthy food culture. It will also enhance nutritional quality of food through selected additives and improvements to the way the body digests and absorb food. Packaging and food safety: Developing smart packaging to optimize product shelf life has been the goal of many companies. Such packaging system would be able to repair small holes/tears, respond to environmental conditions (temperature, moisture) changes and alert the customer whether the food is contaminated. Nanotechnology can provide solutions for this e.g. modifying the permeation behavior of foils, increasing barrier properties (mechanical, thermal, chemical and microbial), improving mechanical and heat resistance properties, developing active antimicrobial and antifungal surfaces and sensing as well as signaling microbiological and biochemical changes. Globally, many countries have identified the potential of nanotechnology in the agrifood sector and are investing a significant amount in it. PLENARY LECTURE NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 MOLECULAR SYSTEMATICS R. Geeta Department of Botany, University of Delhi Taxonomy—the science of naming and organizing the vast diversity of life into groups based on similarities and differences—is an ancient ‘bioinformatic’ science. Taxonomic systems of classification reveal the nested pattern of biological variation. Darwin showed that such a pattern could only be explained by the process of evolution. Evolutionary thinking is the basic to phylogenetic systematics. In reconstructing a dendrogram, we model the branching process of evolution. To modify a familiar statement, nothing makes sense in comparative biology except in the light of phylogenetic analysis. Modern phylogenetics is derived from two strands in biology—taxonomy (more broadly, systematics) and studies of population and molecular variation. Today, molecular phylogenetics is an indispensable tool in the systematist’s hands. “Tree-thinking” is becoming important in biological thinking and research. It is critical to evolutionary and comparative analyses, adding rigour to these fields, much as a statistical analysis does. Phylogenetics has obvious applications in the fields of systematics and evolution, but is equally important in molecular and organismal biology, ecology, epidemiology, anthropology, linguistics and beyond. This presentation would highlight some of these points, with an emphasis on Molecular Systematics. PLENARY LECTURE NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 Biological Synthesis of Inorganic Nanomaterials using Microbes & Plants and their Applications in Imaging, Diagnosis and Therapeutics. Absar Ahmad Biochemical Sciences Division National Chemical Laboratory, Pune-411008 (M.S), India. a.ahmad@ncl.res.in Nanotechnology, in a very short period of time has emerged as one of the most important areas of research and development and shows considerable promise with regards to biomedical, chemical, electronics, energy and drug delivery applications. It dwells upon the changes in properties of bulk materials when at the nanoscale and is used to design different structures, geometries and devices for a wide range of applications. The nanoparticles so far being used in biomedical research are being synthesized by physical and chemical routes. The chemical routes are toxic and require extremes of temperature while the physical ones are expensive and laborious. Unlike these, biological routes for the synthesis of nanoparticles have recently been developed and yield nanoparticles at room temperature and physiological pH with properties such as high stability, water dispersal, fluorescence, etc. which are very difficult to achieve by chemical and physical routes. Moreover, biologically synthesized nanoparticles are naturally protein capped, which prevents their flocculation, thus eliminating the need of any external capping agent which are usually toxic as is seen in chemical routes. Thus, the biosynthesis of nanoparticles is in complete synchronization with the environment and should be further developed in order to obtain nanoparticles of variable sizes, shapes and chemical compositions which will find major applications in cancer research, treatment of cardiovascular disorders, targeted drug delivery systems, etc. In this talk, we describe our research into the use of plants and plant microorganisms in the synthesis of biocompatible, water soluble, fluorescent and protein capped metal, metal sulfide, quantum dots and oxide nanoparticles of different sizes and shapes. In a significant departure from bacteria-based methods for nanomaterial synthesis that have been investigated in some detail, we have shown that plant microorganisms such as fungi and actinomycetes when challenged with aqueous metal ions are capable of reducing the ions both intra and extra- PLENARY LECTURE NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 cellularly resulting in the formation of stable metal nanoparticles. The formation of metal nanoparticles occurs by an enzymatic process and thus, the fungus-based synthesis process is not limited to reduction reactions alone. The versatility of this approach is underlined by our findings that enzymes such as sulphite reductase, nitrate reductase and hydrolyzing proteins are secreted by the fungi in response to metal stress thereby leading to the possibility of synthesis of quantum dots, metal nanoparticles and nanooxides. The biosynthesis of nanooxides using fungi is exciting since the synthesis occurs at room temperature and thus could be of considerable commercial value. Fungi enjoy a close symbiotic relationship with plants and hence, it is quite likely that plant extracts may also possess useful biomolecules which not only carry out the range of biotransformations listed above but also control the shape of nanoparticles. We have studied a number of plant extracts for realizing metal nanoparticles and have observed that the Geranium and Lemongrass extracts result in shape modulated gold nanoparticles. In particular, the reaction of aqueous gold ions with Lemongrass extract resulted in the large-scale synthesis of gold nanotriangles with interesting near infrared absorption. Potential application of the gold nanotriangles, magnetite and other inorganic nanoparticles in hyperthermia of cancer cells, diagnosis, imaging and therapeutic are being investigated. We have also isolated, purified and completely characterized a range of plant based drugs such as Taxol, Vinblastine, Vincristine and other bioactive secondary metabolites from endophytic fungi and recently initiated further important steps of functionalizing the nanoparticles synthesized by green chemistry approach for targeted drug delivery applications. PLENARY LECTURE NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 FLORISTIC DIVERSITY IN INDIA: AN OVERVIEW M. Sanjappa Ex-Director, BSI & Emeritus Scientist Herbarium & Botanical garden, University of Agricultural Sciences, GKVK, Bengaluru 560 065 The vast geographical, diverse climate and topographical realms of India have resulted in enormous ecological diversity supporting about 8% of the world's biological diversity on 2% of earth's surface making it one of the 12 mega diversity countries in the world. Adding to this there is a very high diversity of human influenced ecosystems including agricultural and pasture lands and impressive range of domestic aided plants and animals. The floral resources of India comprise about 46,340 species representing about 11 % of world's known flora. Number of recorded species in major groups of plants is about 17,643 in angiosperms, 69 in gymnosperms, 1236 in pteridophytes, 2451 in bryophytes, 7182 in algae, 2268 in lichens, 14,588 in fungi and 903 bacteria and viruses. Nearly 28% of these plant species are endemic to India with main centers of endemism in Himalayas, North East India, Western Ghats and Andaman & Nicobar Islands. The north eastern region with about 130 species of primitive flowering plants is considered as cradle of Indian flowering plants. The natural forest covering about 19.27% of total geographical areas are known repositories of bioresources in the form of food, fodder, fuel, timber, medicines, fibres, resins, tannins, dyes, oils, fruits, vegetables, etc. About 8000 species of flowering plants have recorded medicinal uses but only 150 of them are extensively used in indigenous system of medicine like Ayurveda, Siddha, Unani and Amchi. About 320 species of wild relative of crop plants are known to have originated in India thus India is considered as one of the 8 Vavilovian centers of origin and diversification of cultivated plants called “Hindusthan Centre of Origin of Crop Plants”. The rich diversity includes legumes, sugarcane, paper, citrus, brinjal, banana, mango, jute, ginger, cinamomum, cardamom, sesame, etc. Within each of them the genetic diversity is tremendous for example rice varieties grown in India are estimated to belong to 50,000 – 60,000 land races. PLENARY LECTURE NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 Oral Presentation List SR. NO. 1. 2. 3. 4. 5. AUTHORS AHLAWAT S., SAXENA P., AND ABDIN M. Z. TITLE OF ABSTRACT ABIOTIC AND BIOTIC ELICITORS ENHANCE PRODUCTION OF ANTIMALARIAL DRUG ARTEMISININ IN HAIRY ROOT 1 CULTURES OF ARTEMISIA ANNUA L. PLANT BHAURAV T. DANGAT AND BIOCHEMICAL STUDIES IN SOME HABENARIA SPECIES RAJARAM V. GURAV FROM WESTERN GHATS BHOSALE K.S., SHINDE B.P., INFLUENCE OF AM FUNGI ON BIOCHEMICAL CONTENTS LIMAYE A.S. OF TURMERIC (CURCUMA LONGA L.) BOLBHAT S. N., GAWADE B. EFFECT OF MUTAGENS ON DRY BIOMASS, ROOT B.M WADAVKAR D. S., NODULES, SEED AND BIOLOGICAL YIELD AND HARVEST SHENDAGE V.S. AND INDEX IN M2 AND M3 GENERATION OF HORSEGRAM DHUMAL K. N. (MACROTYLOMA UNIFLORUM (LAM.)VERDE) DESHMUKH H.V. Page no. 2 2 3 SCREENING OF TROUBLE SOME WEEDS FROM SATARA DISTRICT FOR THEIR POTENTIAL OF BIOGAS 4 PRODUCTION. INGAWALE MANJUSHA, 6. KARANDE V. C., KARANDE C. T. DIATOMS FROM THOSEGHAR, SATARA DISTRICT (MAHARASHTRA). 5 EFFECT OF ARBUSCULAR MYCORRHIZAL FUNGI AND 7. KADLAG P.R., SHINDE B.P., FARM YARD MANURE ON GROWTH AND PRODUCTIVITY BHOSALE K.S. OF FOUR SUGARCANE VARIETIES 5 THE SEEDLINGS OF CUCUMIS SATIVUS AND CAPSICUM 8. KADOO MONAL R. AND ANNUUM RESPOND IN SPECIES-SPECIFIC AND TISSUE- BADERE RUPESH S. SPECIFIC MANNER WHEN CHALLENGED WITH ABIOTIC 6 AND BIOTIC ELICITORS: I – INDUCTION OF CHITINASES AND
- 1, 3 GLUCANASE 9. KAMBLE PRIYADARSHANI AND V. C. KARANDE BLUE GREEN ALGAE FROM HIGH ALTITUDE REGION AROUND SATARA (MAHARASHTRA) ORAL PRESENTATION LIST 7 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 10. 11. KAMBLE V.V., NAMDAS D.D., KHILARE C.J. KISHORE M.VANDANA & A. DIVERSITY OF SPOROPHYLLS,SORI AND SPORES IN B. KAMBLE PTERIDOPHYTES 12. KSHIRSAGAR AYODHYA D. 13. KUMAR PRAMOD LIMAYE A. S; BHOSALE K. S; 14. DUSHING Y. A. AND S. L. LAWARE 15. MANE P. A., KHILARE C. J. 16. DISTRIBUTION OF SOIL FUNGI IN COTTON FIELDS DIVERSITY OF AQUATIC MACROPHYTES FROM RIVER MULA, PUNE CITY TROPHIC STATUS ASSESSMENT OF BHOJ WETLAND THROUGH ITS BENTHIC POPULATION 7 8 9 9 USE OF PROTEINS ISOLATED FROM ADIANTUM AETHIOPICUM AS BACTERIAL AND FUNGAL AMYLASE 11 INHIBTORS ARBUSCULAR MYCORRHIZAL FUNGAL (AMF) DYNAMICS ALONG A FIELD GROWN LINSEED FROM GONDAWALE B.K. || MERAJ-UL-HAQUE AND ENTOMOPATHOGENIC FUNGI AS MICROBIAL NANDKAR P.B. BIOCONTROL AGENT – A SAFE SOLUTION 12 12 EFFICACY OF INDIGENOUS ARBUSCULAR MYCORRHIZAL 17. NAMDAS D.D. AND FUNGAL INOCULATION ON GROWTH AND NUTRIENT C.J.KHILARE UPTAKE IN SORGHUM VULGARE (PERS.) CV. YESHODA PACHANGANE P. M, ALLELOPATHIC EFFECT OF LEAF EXTRACT OF ACACIA 18. BHOSALE R.S, KHOMANE B. 19. 20. V VULGARE PERS. NOM. ILLEG). PATIL H. S., AVCHAR B. K., INVASION OF WEEDS INTERRUPTING LOCAL AND JAGTAP D. K. BIODIVERSITY OF BARAMATI TAHASIL, DIST-PUNE. PATIL S. D. AND D. R. HISTOCHEMICAL STUDIES ON ANTHERS OF AEGICERAS SHIRKE. CORNICULATA Blanco. PATIL SHANKARAO A., 21. NILOTICA L. ON GERMINATION IN JOWAR (SORGHUM KORANE MADHAVI A., PATIL SMITA V., SALOKHE POOJA A. 22. PATIL, A.M. AND D.A.PATIL 23. ROHAMARE YOGITA, 13 13 14 14 BIOACCUMULATION OF HEAVY METALS BY HYDRILLA VERCILLATA AND INCREASED LEVELS OF LEAD 15 CONTAMINATION BY TYPHA LATIFOLIA PETIOLAR ANATOMY OF SOME HITHERTO UNSTUDIED ACANTHACEAE ESSENTIAL OIL COMPOSITION OF TRACHYSPERMUM ORAL PRESENTATION LIST 16 16 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 NIKAM T.D.AND DHUMAL AMMI (L.) FRUITS CULTIVATED IN PUNE (MAHARASHTRA) K.N. SABALE RUPALI, BANERJEE KAUSHIK, DASGUPTA 24. SOMA, UTTURE SAGAR, DEGRADATION KINETICS AND SORPTION STUDY OF KRESOXIM METHYL ON FOUR DIFFERENT SOILS 17 OULKAR DASHARATH, ADSULE PANDURANG SCREENING OF FRESHWATER GREEN ALGAE 25. SAGAR S. S. ENTEROMORPHA INTERMEDIA FOR ITS ANTIMICROBIAL 18 ACTIVITY. SALUNKHE VARSHA P., SAWANT INDU S., 26. BANERJEE K., RAJGURU IN SITU BIODEGRADATION OF PESTICIDES AND FOOD YOGITA R., WADKAR SAFETY 19 PALLAVI N., OULKAR D. P., SAWANT S. D. SAXENA PARUL, 27. AHLAWAT SEEMA AND ABDIN MALIK ZAINUL 28. 29. INFLUENCE OF AGROBACTERIUM RHIZOGENES ON INDUCTION OF HAIRY ROOTS FOR ENHANCED PRODUCTION OF WITHAFERIN A IN WITHANIA 20 SOMNIFERA L. PLANT SHINDE VAISHALI AND THREE INTERESTING AERO AQUATIC FUNGI FROM PAWAR A.B. MAHARASHTRA. SONAR, B. A. AND CHAVAN, EFFECT OF BORON TOXICITY ON SEED GERMINATION IN P. D. THREE HIBISCUS SPECIES ORAL PRESENTATION LIST 21 21-22 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 Poster Presentation List SR. NO. 1. 2. 3. 4. TITLE OF ABSTRACT AHIWALE N.H., SAVANT S.H., PATIL ANTINUTRITIONAL ACTIVITY IN SOME PTERIDOPHYTIC A.S. AND PATIL R. B PLANTS BAGWAN S. A. AND KORE B. A. BRYOFLORA OF KAS PLATEAU AND ADJOINING AREA 23 BAGWAN S.A. AND KORE B. A. CYTHODIUM TUBEROSUM, KASH. (LIVERWORT)- A NEW 24 6. 7. 8. Page no. 23 HOST OF EUDONIA MERCURELLA. BHALERAO E.B. AND DHUMAL K.N. CHAUGULE G. P. AND. NALWADE 5. Poster AUTHORS A. R CHAVAN Y. R., THITE S. V., APARADH V. T. AND KORE B. A. GAIKWAD S. E. AND SHINDE R. A. ORGANIC CULTIVATION OF STRAWBERRY THROUGH 24 APPLICATION OF FERN FROND BIOMASS IN-VITRO MULTIPLICATION OF TYLOPHORA INDICA BURM. 25 F. AN ENDANGERED HIGH VALUED MEDICINAL PLANT PHYTOCHEMICAL ANALYSIS OF SOME WEEDS EFFECT OF SALT & DROUGHT STRESS ON TWO VARIETIES OF 26 26 CAPSICUM ANNUM GROWN IN MAHARASHTRA GAIKWAD Y. B. AND BHAWANE G. PROTECTIVE EFFECT OF ETHANOLIC EXTRACT OF LACTUCA P. SATIVA AGAINST D-GALACTOSE INDUCED OXIDATIVE 27 STRESS IN SILKWORM BOMBYX MORI 9. 10. GANORKAR R. P. MAHARASHTRA) GAWADE B. B, BOLBHAT S.N, AN EFFICIENT REGENERATION AND MULTIPLICATION OF WADVKAR D.S, SHENDAGE V.S. WITHANIA SOMNIFERA DUNAL,FROM NODAL EXPLANTS 11. GHADGE S. J. AND KARANDE V. C. 12. FLORISTIC STUDY OF SHIRUR REGION (PUNE, 28 28 BLUE GREEN ALGAE FROM KARAD, DISTRICT SATARA 29 GHATGE M.M., SALUNKHE V.S., FUGAL DIVERSITYAT KADEGAON TOWN OF SANGLI DISTRICT (M.S.) 30 JADHAV R.R. INDIA. 13. GHORPADE B. A. , CHAVAN J. J. GOLE A.A., RAMUGADE M.A., 14. BHAGWAT H.R., GAIKWAD S.S., PHYTOCHEMICAL SCREENING OF VARIOUS EXTRACTS OF 30 ‘SAPTARANGI’ (SALACIA CHINENSIS L) STUDY OF STOMATAL DIVERSITY IN SOME ANGIOSPERMIC FAMILIES MOHITE P.C. AND APARADH V. T. POSTER PRESENTATION LIST 31 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 15. GURAV K. D., THITE S.V., PATIL P. R., AND KORE B.A. 16. J. A. MAGDUM 17. 20. 21. GYMNOSPERM SPECIES 32 JADHAV A.R., KANASE R.M., NUTRITIONAL STATUS IN POMEGRANATE AND CUSTARD 33 KADAM D.A. APPLE LIST OF FLOWERING PLANTS (DICOT) FROM KHAMBATAKI MEDICINAL HERBS, BEING USED AS NATURAL KADAM. ABHIJEET, JAKHI.P.S. HEALERS,FOR FUNGAL INFECTION JADHAV S.N, KADAM D.A., WEED CELOSIA ARGENTEA LINN. USED US AYURVEDIC KADAM. ABHIJEET, JAKHI.P.S. MEDICINE BY RURAL PEOPLE FROM SATARA TALUKA (MS). JADHAV S.N., JAKHI P.S, SACRED GROVES A MINOR FOREST PRODUCT RESERVE IRANNAWADI REHUKA FROM SATARA DISTRICT.(MS) JAGTAP A. A., DESAI M. S., SURVIVAL ABILITY OF CARBENDAZIM RESISTANT ISOLATE P., ANDOJI Y. S. AND KAMBLE S.S. JAMADAR A.M., KHADE S.K. AND KARANDE V.C. JOSHI. A. S. AND CHAVAN P. D. 35 36 37 TURMERIC SEED GERMINATION OF PEDALIUM MUREX L. 37 38 COLEUS FORSKOHLII (WILLD.) TO FOLIAR APPLICATION OF CCC (CHLORO CHOLIN CHLORIDE). 25. KADAM P. S. KADAM A. P. , SALUNKHE N. B., 26. APARADH V. T., KADAM D. A., CHAVAN J. J. KADAM P. K.., DHUMAL N. C., 27. DHUMAL T. C., BARGE A. N., Y. A. GHULE AND SHINDE H. L. 28. 34 OF SCLEROTIUM ROLFSII CAUSING RHIZOME ROT OF RESPONCES OF PLECTRANTHUS BARBATUS (ANDR.) SYN. 24. 33 GHAT OF SATARA DISTRICT. JADHAV S.N, KADAM D.A., 22. WALAVADE M. N., DORUGADE S. 23. 32 FUNGAL BIODIVERSITY IN SEEDS OF SOME CEREALS 18. JADHAV D. AND BADAVE G.N. 19. PRELIMINARY PHYTOCHEMICAL ANALYSIS OF SOME KADAM, P.A., SHINDE VAISHALI, CHAVAN, Y.R, AND MUNDADA P.S. DIVERSITY AND PHOTOSYNTHETIC PIGMENTS EVALUATION OF TOTAL PHENOLIC CONTENT IN DIFFERENT 39 39 PARTS OF SOLANUM XANTHOCARPUM PRELIMINARY INVESTIGATION OF SECONDARY 40 METABOLITES FROM VIRAL DISEASES PRELIMINARY STUDIES OF THE ANTIFUNGAL ACTIVITIES OF SOME MEDICINAL PLANTS AGAINST AUREOBASIDIUM PULLULANS POSTER PRESENTATION LIST 40 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 29. KAMBLE NILAM , SHINDE JYOTI, SHINDE ROHINI KAMBLE P.B., DEO A. V. AND 30. KULKARNI D.K. 31. 32. KAMBLE VAISHALI S. AND JADHAV VARSHA D. KANASE R.M, JADHAV A.R, KADAM D.A KHANDARE N.K., KAMBLE S.S. AND 33. KHANDARE S.N. ALLELOPATHIC EFFECT OF ARAUCARIA AND THUJA ON 41 WHEAT AND JAWAR GERMINATION AND GROWTH ETHNO-MEDICINAL PLANT RESOURCES USED AS EVM FOR 42 WOUND HEALING IN BHOR REGION OF PUNE DISTRICT, MAHARASHTRA. NUTRITIONAL STUDIES OF SOME NON CONVENTIONAL 43 LEAFY VEGETABLES OF KOLHAPUR DISTRICT OF MAHARASHTRA NUTRITIONAL STATUS OF WILD JUJUBE FRUIT EFFICACY AND EFFECT OF PASSAGE ON THE 43 44 DEVELOPMENT OF CARBENDAZIM RESISTANCE AGAINST ALTERNARIA ALTERNATA CAUSING ROOT ROT OF FENUGREEK KHARADE AMIT S, JADHAV 34. SANGITA S., SHINDE H. L, JADHAV S.N, KADAM D.A AND SAGAR S. S. 35. 36. 37. KHILARE C.J. AND KULKARNI P.D. PHYTOCHEMICAL SCREENING OF COMMELINEA 45 BENGALENSIS & CYANOTIS CRISTATA PHYTOCHEMICAL SCREENING OF CYTHOCLINE MANILIANA 45 C.P.RAJU &R.R.V.RAJU FOR SECONDARY METABOLITES KHUNTE SUNIL, KHUNTE TEJAS & PHYTOREMEDIATION - A NOVEL STRATEGY FOR MAGDUM JAYSHREE ENVIRONMENTAL CLEANUP USING PLANTS KOLAR F. R., GHATGE S. R. AND INFLUENCE OF CERTAIN CHEMICALS ON VASE LIFE OF DIXIT G. B. DELPHINIUM MALABARICUM (HUTH) MUNZ. KULKARNI A.A, GAYKAR B. M & ETHNOBOANICAL STUDIES CONCERNED WITH MEDICINAL 38. SURYAVANSHI S. S 46 47 48 PLANTS IN TRIBAL AREAS OF AHMEDNAGAR DISTRICT(MAHARASHTRA) SURVEY OF GAMOPETALOUS ANGIOSPERMS FROM 39. KULKARNI SANGITA AND AUTI B.K. 40. KULKARNI, S.S. AND CHAVAN, P.D. 48 AHMEDNAGAR STUDY OF EFFECT OF WATERLOGGING ON ROOT ANATOMY OF RAGI AND RICE. POSTER PRESENTATION LIST 49 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 41. KUMBHAR P.R., PAWAR P.R., KADAM D.A. MANE N. B., KHILARE C. J., D. D. 42. NAMDAS, NAIK V. V. AND APARADH V. T. 43. 44. KHILARE C. J. (L. ) DUNAL MANE PRADNYA A., D. D. NAMDAS Arbuscular Mycorrhizal Fungal (AMF) Dynamics along a C. J. KHILARE field grown Linseed from Gondawale B.K. II S.S. FROM SATARA DISTRICT [MAHARASHTRA], INDIA. SHINDE H. L. AND APARADH V. T. NIMBALKAR D.N ., MUNDADA P.S., KADAM D.A. PANDIT S.S., JADHAV S.M., 50. SATPUTE M.D., SURVE M.M. AND NIKAM V.K. PATIL A. S., SAVANT S.H., AHIWALE 50 50 51 51 SHAMGAON GHAT, KARAD, DIST. SATARA . ECOLOGICAL STUDIES ON PATESHWAR SACRED GROVE 48. GHATAGE S. Y., BHOSALE P. R., 51. A CHEACK LIST OF ANGIOSPERMIC FLORA OF MOHITE S.A., CHAVAN N.S., SATHE NIKAM K. S., SABALE S. R., 49 PARTS OF ASHWAGANDHA. MYCORRHIZAL ASSOCIATION OF WITHANIA SOMANIFERA 47. MOTE MANJIRI R. 49. COMPARATIVE PHYTOCHEMICAL VARIATION IN DIFFERENT MANE N. B., NAMDAS D. D. AND 45. MOHITE B.D. 46. STUDY OF ENZYMATIC STATUS IN WILD JUJUBE FRUIT BIODIVERSITY OF BASIDIOMYCETES FUNGUS IN 52 52 PANVEL. SCREENING OF PLANT WEAPON PRODUCTION AT THE 53 STAGE OF INFECTION IN SORGHUM VULGARE PERS. GREEN SYNTHESIS OF SILVERNANOPARTICLES USING THE 53 LEAF EXTRACT OF BAMBUSEAE AND STUDY OF ITS ANTIMICROBIAL ACTIVITY PHYTOCHEMICAL ANALYSIS OF MEDICINAL PLANT 54 MOMORDICA SPECIES. STUDY OF TOXICITY IN SOME CUCURBITACEAE MEMBERS 54 N.H., APARADH V.T. PATIL BHIMRAO J., KAMBLE 52. SUHAS, MADANE ATUL, PAWAR BIODIVERSITY AND ANTIMICROBIAL ACTIVITIES OF 55 AGRICULTURAL WEEDS IN KARAD TAHSIL DHANAJI AND SHINDE ARCHANA 53. PATIL C. R. AND PATIL D. A. 54. PETIOLAR ANATOMICAL STUDIES IN SOME RUBIACEAE 55 PATIL CHANDRAHAS AND PATIL DIVERSITY IN GALAXY OF FUNGI OF WORLD HERITAGE SITE 56 SUNETRA – KAS (DISTRICT - SATARA.) POSTER PRESENTATION LIST NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 PATIL DIPALI T., KADAM A. S, 55. THITE S.V. AND KORE B.A. 56. PATIL P.D., TUPE S.B AND SHINDE R.A. PATIL R.B., CHAVAN P. B., 57. VARNARAYAN J. S., GURAV S. B., QUALITATIVE ANALYSIS OF SECONDARY METABOLITES 57 FROM SOME FILICALES MEMBERS. TO STUDY THE COMPARATIVE ENZYMATIC STATUS OF 57 FAMILY CUCUBITACEAE. COMPARATIVE ANALYSIS OF TWO CULTIVATED VARIETIES 58 OF SORGHUM UNDER DIFFERENT STRESS CONDITIONS SHINDE R. A. AND KADAM D. A. 58. PATIL S. C. 59. PATIL S. S. 60. 64. VAR. Co.740 AND Co. 8014. V.C. and KARANDE C.T. SATARA CITY. AND SHIMPALE V. B. 63. EFECTS OF LONG CHAIN FATTY ALCOHOL ON SUGARCANE LIMNOLOGICAL STUDIES OF SOME WATER BODIES IN 61. KSHIRSAGAR P. R., UMDALE SURAJ 58 KOLHAPUR PART – I PATIL S.V, PAWAR S.D., KARANDE PAWAR N. V., NIMBALKAR M. S., 62. FLORISTIC COMPOSITION OF RAJARAM COLLEGE CAMPUS BHUDARGAD FORT: A HIDDEN TREASURE OF FLORISTIC 59 60 60 DIVERSITY FROM NORTHERN WESTERN GHATS PAWAR P. R., KUMBHAR P.R., STUDY OF ENZYMATIC STATUS IN ANNONA SQUAMOSA KADAM D. A. AND PUNICA GRANATUM PAWAR UDAY, JOSHI, A. J. MEASURING HALOPHYTE DVERSITY ALONG SEA COAST OF 61 61 GUJARAT A CASE STUDY SABALE R.R., MOHITE S.A., ANGIOSPERM DIVERSITY OF NANDAGIRI HILL FROM JADHAV P.S SATARA DISTRICT [MAHARASHTRA], INDIA. SALUNKHE N. B., KADAM A. P. COMPARATIVE STUDY OF PHOTOSYNTHETIC PIGMENT 65. AND CHAVAN J. J. 62 62 AND PHENOLIC CONTENT IN DIFFERENT SPECIES OF BARLERIA IN SATARA CITY. SAMANEH AKHOONDI 66. DARZIKOLAEI AND SAEED SHIRZADIAN 67. SIX NEW AND INTERESTING MOSS RECORDS TO THE 63 IRANIAN BRYOFLORA SAVANT S.H., AHIWALE N.H., ANTINUTRITIONAL ACTIVITY OF SOME GYMNOSPERM GHULE Y. A. AND GAIKWAD S.S. SPECIES POSTER PRESENTATION LIST 64 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 68. SAWANT V. S. SHEDAGE N. V., SHAIKH S. S., 69. SHINDE H. L., PATIL P. R., CHAVAN J. J. 70. SHENDAGE S. M. AND YADAV S. R. SHINDE B.P., KADLAG P.R., 71. BHOSALE K.S. 72. 73. 74. SHINDE M. V., KADAM A. P. AND SHINDE R. A 77. EFFECT OF WATER HOLDING CAPACITY AND SOIL PH ON AESTIVUM L.) AND CHICKPEA (CICER ARIETINUM L.) SEED COAT MORPHOLOGY OF INDIAN SPECIES OF 66 BARLERIA L. (ACANTHACEAE) RESPONSE OF AM FUNGI ON CHLOROPHYLL AND NUCLEIC 66 ACID CONTENT IN FOUR SUGARCANE CULTIVARS ALLELEOPATHIC EFFECT OF BASIODIOMYCETES ON 67 GERMINATION AND GROWTH OF TRITICUM AESTIVUM VAR LOKVAN MINAKSHI FIELD AT PATAN,(DIST-SATARA, M.S)INDIA. THITE S.V., MUNDADA P.S., KORE A PROBABLE BACTERIAL FUNGICIDE FOR POWDERY B.A. MILDEW DISEASES KORE B. A. AND PAWAR A.B. 65 SEED GERMINATION PARAMETERS OF ‘WHEAT’ (TRITICUM AEROBIOLOGICAL DIVERSITY OVER GROUNDNUT CROP THORAT P. L., SHINDE VAISHALI, 64 GROWTH SHINDE MANJUSHA, MAHAJAN 75. THOKE, R. B. 76. STUDIES ON THE EFFECTS OF AIR IONS ON PLANT 68 69 AEROSPORA SURVEY OF PESHWE PARK, PUNE 69 COMPARATIVE STUDY OF WATER QUALITY USING 70 PHYSICO-CHEMICAL PARAMETERS OF KAS LAKE AND PHUTAKA TALAV FROM SATARA, MAHARASHTRA. TUPE SACHIN B., PATIL P. D., PHYTOCHEMICAL SCREENING IN SOME CUCURBITACEAE THOKE R.B AND APARADH V.T. MEMBERS 70 EFFECT OF AGERATINA AND CATUNAREGAM LEAF 78. VAIDYA, R.R AND DHUMAL K.N. LEACHATES ON SEED GERMINATION AND SEEDLING 71 PHYSIOLOGY IN VIGNA RADIATA L 79. 80. YADAV N.T., MUNDADA P.S., KADAM D.A. SYNTHESIS OF UREA COATED HYDROXYAPATITE 72 NANOPARTICLES AND THEIR POTENTIAL USE AS A NANOFERTILIZERS YADAV SANYOGITA, PAWAR S. G., EFFECT OF SULPHER ON GERMINATION OF GROUNDNUT THOKE R. B.AND GHANAWAT N. A. AND GRAM SEEDS POSTER PRESENTATION LIST 72 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY JANUARY 18th - 19th, 2013 ORAL ABSTRACTS NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 ABIOTIC AND BIOTIC ELICITORS ENHANCE PRODUCTION OF ANTIMALARIAL DRUG ARTEMISININ IN HAIRY ROOT CULTURES OF ARTEMISIA ANNUA L. PLANT S. Ahlawat**, P. Saxena, and M. Z. Abdin Centre for Transgenic Plant Development, Department of Biotechnology, Faculty of Science, Jamia Hamdard, New Delhi 110062, India Artemisinin is frequently used for ACTs to cure the drug resistant malaria in Asian subcontinent and large swath of Africa. The low concentration of artemisinin in A. annua L. is the main problem in commercialization of artemisinin based drugs. In our laboratory, the hairy root system for A. annua L. plants was developed by using the Agrobacterium rhizogenes LBA 9402 strain. High yielding hairy root lines were selected on the basis of artemisinin content and specific growth rate. The integration of rol B gene (from Agrobacterium rhizogens LBA 9402) and its copy number was confirmed by PCR and Southern Blot analysis, respectively. Hairy root initiation conditions like temperature, illumination conditions, co-cultivation time etc. were optimized. Various abiotic (salicylic acid, methyl jasmonate, calcium chloride, silver nitrate and copper sulfate) and biotic (cell homogenates and culture filtrates of Alternaria alternata, Curvularia limata, Fusarium solani and Piriformospora indica) elicitors were tested at different concentrations to enhance artemisinin yield. Maximum enhancements in artemisinin yield (11.4±0.26 mg L-1 and 15.6±0.15 mg L-1, respectively) were obtained, when methyl jasmonate (100 µM) and cell homogenate of P.indica (3% v/v) were added separately to hairy root cultures. Dual elicitation strategy by combined addition of these two elicitors resulted in artemisinin yield of 19.8±0.11 mg L-1 in comparison to control cultures (8.5±0.02 mg L-1). The present study indicates the potential of this biotechnology-based process for large-scale production of artemisinin. Keywords Sesuiterpene, malaria, cancer, A. annua L., artemisinin, hairy root cultures, in vitro ORAL SECTION Page 1 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 Biochemical studies in some Habenaria species from Western Ghats Bhaurav T. Dangat and Rajaram V. Gurav Department of Botany, Shivaji University, Kolhapur schndangat@gmail.com Being one of the most specialized groups of plants of the world, the member of orchidaceae provides fascinating physiological facts. Biochemical analysis was carried out from the mature leaves of 16 Habenaria species. Chlorophyll, Carotenoid, Flavonoid, Polyphenol contents and activity of an enzyme Catalase and Peroxidase was analyzed. Comparative analysis was described and presented here. Biochemical Parameters studied Highest Lowest Total Chlorophyll content H. foliosa A.Rich. H. diphylla (Nimmo) Dalzell Chlorophyll a/b ratio H. longicornu Lindl. H. ovalifolia Wight Carotenoids H. furcifera Lindl. H. diphylla (Nimmo) Dalzell Flavonoids H. foliosa A.Rich. H. marginata Colebr. Polyphenols H. heyneana Lindl. H. marginata Colebr. Activity of Catalase H. commelinifolia H. roxburghii Nicolson (Roxb.) Wall. ex Lindl. Activity of Peroxidase H. longicornu Lindl. H. roxburghii Nicolson INFLUENCE OF AM FUNGI ON BIOCHEMICAL CONTENTS OF TURMERIC (Curcuma longa L.) *K.S. Bhosale, B.P. Shinde, *A.S.Limaye * P.G. Department of Botany Nowrosjee Wadia College Pune 411 001 India P.G. Department of Botany Fergusson College Pune 411 004 India The rhizomes of turmeric (Curcuma longa L.) plants were grown with and without mycorrhizal fungi in pots (12x9 inch). The autoclaved soil was added in all the pots. At the time of plantation of turmeric 100 g of inoculum containing propagules of Glomus convolutum and ORAL SECTION Page 2 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 G. fasciculatum were added in each experimental pot. For present investigation three replicates of both control and experimental plants were maintained. The rhizomes of turmeric were grown in all the pots after washing by 0.5 % HgCl2 for five times. The plants were watered after every 5th day for six months continuously. The leaves and rhizomes of turmeric plants were used to estimate the biochemical contents after 60th, 90th, 120th and 150th days. The biochemical contents like chlorophyll a, chlorophyll b, total chlorophyll, were estimated from leaves. Proteins and total soluble carbohydrates were estimated from the rhizomes of turmeric. The amount of chlorophylls, proteins as well as total soluble carbohydrates was recorded more in mycorrhizal plants more than non mycorrhizal plants. The amount of chlorophylls were increased up to 120th day and at last stage i.e. on 150th day it was decreased but the proteins and total soluble carbohydrates continuously increased during all the growth stages. Keywords: AM fungi, Turmeric, chlorophyll, Proteins, Total soluble carbohydrates. Effect of mutagens on dry biomass, root nodules, seed and biological yield and harvest index in M2 and M3 generation of horsegram (Macrotyloma uniflorum (Lam.) Verdc) Bolbhat S. N., Gawade B.B., Wadvakar D.S., Shendage V.S. and Dhumal K. N.* Department of Botany, Dada Patil Mahavidyalaya, Karjat, Dist- Ahmednagar-414402 (M.S.), Department of Botany, University of Pune, Pune-411007 (M.S.) India* bolbhats@gmail.com In present investigation the seeds of horsegram (Macrotyloma uniflorum (Lam.) Verdc) cv. Dapoli Kulthi- 1 were treated with gamma radiation (100, 200, 300 and 400Gy), EMS (0.2, 0.3, 0.4 and 0.5 %) and combination treatments. The mutations affecting dry biomass, root nodules, seed and biological yield and harvest index in M2 and M3 generation were studied. Data obtained on dry biomass of shoot and root in M2 generation showed significant positive as well as negative influence over control. While in M3 generation it was on par with M2. Higher number of nodules on roots as well as higher nodule fresh weight per plant in M2 and M3 generation was recorded in horsegram. All the treatments of gamma radiation and combination ORAL SECTION Page 3 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 (except few) showed negative effect. But almost all the treatments of EMS had shown stimulatory effect. In M2 and M3 generation mean values for seed yield per plant, biological yield and harvest index decreased with few exceptions in all mutagen treated populations as compared to their controls. Keywords- Biological yield, Harvest index, Horsegram, Mutagens, Seed yield SCREENING OF TROUBLE SOME WEEDS FROM SATARA DISTRICT FOR THEIR POTENTIAL OF BIOGAS PRODUCTION. H.V.Deshmukh Department of Microbiology. Yashawantrao Chavan Institute of Science, Satara. 415001, Maharashtra. (India) deshmukhhv@gmail.com Most of the developing countries are facing energy problem mainly due to over population, Industrialization and scarcity of fossil fuels. Now there is an urgent need to solve this problem. Use of renewable energy resources such as wind, solar, tidal and bioenergy are very good alternatives for production of energy. Out of these resources bioenergy seems to have a great potential because of ubiquitous presence of plenty of plant biomass. Weeds are undesirable or troublesome plants. These plants cause a great nuisance; invade large agricultural land and some aquatic weeds causes water pollution .These weeds are very difficult to eradicate. The present work has been undertaken to study the potential of some common weeds abundantly found in Satara region to produce biogas. The plants were used in the original form and after mild alkali treatment. There was marked increase in biogas production after pretreatment in almost all the weeds. Experiments were carried out in 1-L digester flasks in the batch process; measurement of biogas was done by water displacement method. The biogas production was recorded up to 30 days. In general Cynodon dactylon , Ipomoea carnea, Blumea lacera, Striga gesnerioide, Parthenium hysterophorus and Lantana camera weeds showed the best biogas production results. The use of these weeds controls the weeds ORAL SECTION Page 4 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 from the fields and can be made to supplement the conventional substrate like dung in rural areas to augment the biogas production. Diatoms from Thoseghar, Satara district (Maharashtra ). Manjusha Ingawale, V. C. Karande*, C.T.Karande** Department of Botany, Kisan Veer Mahavidyalaya, Wai. * Department of Botany, Yashavantrao Chavan Institute of Science, Satara. ** Miraj Mahavidyalaya, Miraj, Dist.Sangali. Present paper deals with study of some members of Bacillariophyceae collected from water bodies of Thoseghar, Satara district. Thoseghar is a small village 20 Km from Satara city at the edge of the Kokan region in Western Maharashtra. It is famous for waterfall. There are a series of waterfalls. Some of them 15 to 20 meters and one of 500 meters in height. This study has shown presence of thirty diatoms belonging to two orders Centrales and Pennales. These species belong to twelve genera namely Cyclotella, Achnanthes, Fragilaria, Synedra, Pinnularia, Navicula, Gomphonema, Eunotia, Hantzschia, Nitzschia, Cymbella, Amphora while Eunotia is found dominant among these diatoms. These diatoms are being reported for the first time from the study area. EFFECT OF ARBUSCULAR MYCORRHIZAL FUNGI AND FARM YARD MANURE ON GROWTH AND PRODUCTIVITY OF FOUR SUGARCANE VARIETIES * P.R. Kadlag, B.P. Shinde, K.S. Bhosale Department of Botany Dr. Babasaheb Ambedkar College Mahad 402 301 India P.G. Department of Botany Fergusson College Pune 411 004 India P.G. Department of Botany Nowrosjee Wadia College Pune 411 001 India The present investigation was carried out on Sugarcane (Saccharum officinarum L.) plants at the Vasantdada Sugar Institute (VSI) Pune. Four cultivars of sugarcane plants used for experiment were CoVSI- 9805, CoC- 671, Co- 86032 and VSI- 434. All these cultivars were ORAL SECTION Page 5 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 inoculated with the arbuscular mycorrhizal fungi and farm yard manure (FYM). At the time of cultivation one kg. of FYM and 100 g of inoculum containing propagules of Glomus fasciculatum and Scutellospora minuta was added in soil. The plants of sugarcane were grown with AM fungi alone, FYM alone and in combination of both AM fungi and FYM and without AM fungi and FYM. Three replicates of both control and experimental plants were maintained. Plants were grown for one year. These plants were assessed for various growth parameters like number of tenders, number of leaves, number of internodes, height of shoot and fresh weight of stem. The assessment of plants was carried out at the interval of 60 days starting from 90th day i.e. on 90th, 150th, 210th and 270th days respectively. The plant inoculated with AM fungi, FYM and dual inoculation of AM and FYM showed increased fresh weight of plants and various growth parameters. The combination of AM fungi and FYM showed better growth and yield than AM fungi and FYM alone. The seedlings of Cucumis sativus and Capsicum annuum respond in species-specific and tissue-specific manner when challenged with abiotic and biotic elicitors: I – Induction of chitinases and
- 1, 3 glucanase Monal R. Kadoo and Rupesh S. Badere* Department of Botany, Hislop College, Temple Road, Civil Lines, Nagpur- 440 001 (India) rsbadere@rediffmail.com, Like any other crop plants, Cucumis sativus and Capsicum annuum also face huge yield loss due to various diseases. Hence, a great deal of cost is incurred for disease management in these crops. Moreover, the fungicides used in this regard pose an environmental threat. Induction of systemic acquired resistance (SAR) of plants by synthetic chemicals is seen as an environment-friendly alternative of protecting plants against invading pathogens. However, prior to this a pilot study is needed to assess the response of these crops towards elicitation by the known biotic and abiotic elicitors. Chitinases and
- 1, 3 glucanase are regarded as the markers of SAR. Hence, we studied the induction of these enzymes by various biotic and abiotic ORAL SECTION Page 6 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 elicitors in the seedlings of C. sativus and C. annuum. It was found that all the elicitors used induced the activity of Chitinases and
- 1, 3 glucanase to a significantly high level. Initially, the response was found to be low which gradually increased and reached the peak only after 50h of elicitation. However, the induction of these enzymes was found to be dependent upon various factors like elicitor, seedling part and plant species. BLUE GREEN ALGAE FROM HIGH ALTITUDE REGION AROUND SATARA (MAHARASHTRA) Kamble Priyadarshani* and Mrs. V. C. Karande Department of Botany, Yashavantrao Chavan Institute of Science, Satara-415001 A comprehensive study on occurrence of blue-green algae from various habitats of high altitude region like Ajinkyatara fort, Kas plateau, Thoseghar, Parali, Yevateshwar has been presented in this paper. A total number of 30 taxa belonging to 3 orders were identified. Among these 11 species were heterocystous and 19 non heterocystous forms were recorded. Out of these 30 species 26 forms are filamentous while 4 are unicellular. Results indicated that filamentous form Oscillatoria dominated the population of blue green algae of study area. Key words: Blue green algae, filamentous, high altitude, heterocystous, Oscillatoria. Distribution of soil fungi from soils of Yelewadi. District, Satara V.V. Kamble 1 D.D.Namdas 2 C.J.Khilare3 Dahiwadi College Dahiwadi Soil fungi were isolated from rhizosphere and non rhizosphere soil types in the field of cultivated Cotton (Gossypium herbaceum) varieties Varlaxmi and BT RASI II at Yelewadi of Satara District, giving their physical characteristics and distribution of fungi during winter season. A total number of 08 genera and 11 species were isolated and moisture content 11.4% to 14.5%. Deuteromycotina members were dominant. Aspergillus was dominant genus in all samples. ORAL SECTION Page 7 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 DIVERSITY OF SPOROPHYLLS, SORI AND SPORES IN PTERIDOPHYTES *M.VANDANA KISHORE & *A. B. KAMBLE Department of Botany and Plant Protection Sadguru Gadage Maharaj College, Karad Maharashtra- 415 124. (mekishore2000@yahoo.com) The term pteridophyta (Greek- Pteron-feather; Phyton-plant) i.e. plants bearing feather-like leaves. The pteridophytes include about 400 genera and 12,000 species in the world. The pteridophytes are connecting the link between the lower plants and higher plants. Pteridophyta is a group showing evolution in various systems of plant body e.g. anatomy, morphology and reproduction. In the present investigation the attempt has been made to study the diversity of sporophylls, sori and spores in some pteridophytes. For the study, survey is carried out for the diversity of pteridophytes in the different seasons in 2011-2012 from satara district. The sporophylls of different genera are collected and the structure of sori and spores is studied in the laboratory. For the study of diversity; in the present investigation a few genera are selected. In the study it has been observed that sori occur in sporophytic generation. These sori are produced in linear fashion or circular fashion or they may be arranged in rows either parallel or oblique to costa. By their arrangement they produce very good ornamentation on sporophylls. The location may be margial or set away from margin of lamina. The shapes, arrangements and locations of the sori are often valueable clues in the identification of fern taxa. In the present investigation the following genera; collected from Satara district are taken for the study of diversity of sporophylls, sori and spores. Asplenium, Adiantum sp, Blechnum, Cheilanthes sp, Dicksonia, Microsorium sp, Marattia, Pteris sp, Tectaria. Keywords - pteridophytes, diversity, sori, sporophylls, spores. ORAL SECTION Page 8 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 DIVERSITY OF AQUATIC MACROPHYTES FROM RIVER MULA, PUNE CITY Ayodhya D. Kshirsagar* Department of Botany, C.T.Bora College, Shirur, Pune-412210 (MS, India). drayodhya11@gmail.com The present study deals with the aquatic macrophytes diversity from river Mula from Pune city from selected sampling stations (Stations I, II and III) isolated during October 2007 to September 2008. During present study 74 species of plants was recorded from Mula river flowing through the Pune City. Species among plant, indicative of organic enrichment are Eichhornia crassipes, Pistia stratiotes, Alternanthera sessilis, Persicaria glabra, Cyperus compressus, Amaranthus tricolor are found in large population at station II and III at Mula river. Eichhornia crassipes and Pistia stratiotes as weeds was predominant at sampling stations which are the most tolerant and could be regarded as pollution tolerant aquatic macrophytes and be used as a biological indicator for water pollution. These studies indicate that, aquatic macrophytes species are specific to the environmental quality and therefore can be used as agent in bioremediation. Key words: aquatic macrophytes, Mula river, biological indicator, diversity, water pollution. Trophic status Assessment of Bhoj Wetland through its Benthic population Pramod Kumar Department of Environmental Sciences and Limnology, Barkatullah University, Bhopal, India pramodlimno2004@yahoo.com In order to assess the trophic status of a multipurpose aquatic resource (Bhoj wetland), the main attention was focused on its benthic population as it gives a fare idea about the functioning of the system besides assessing its trophic status, necessary from the point of view of adopting a management strategy. Upper basin of Bhoj wetland still forms the lifeline of ORAL SECTION Page 9 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 Bhopal by way of providing potable water to the local habitants however, looking to its multiple uses it has been notified as per the guidelines of Ramsar convention as one of the important bird sanctuary. Physiochemical analysis of mud water interface was carried out following the standard methods as given in Adoni (1985) and APHA (2000). Sediment samples were collected from a circle of 50 meters at each selected sites in both the basins. For qualitative and quantitative study of benthic fauna mud samples were collected by means of Peterson grab mud sampler (270 cm2) in polyethylene bags. The dredged material was sieved through metallic sieves of 1mm and 0.5mm pore size. All the benthic organisms sorted out manually for and preserving the samples with 4% formaldehyde solution. The results have been expressed in organisms/m 2. The identification of macro-invertebrates was carried up to the species level for their taxonomic and various morphometric characters with the help of standard taxonomic references. Considerable variations in benthic fauna have been recorded in the water upper and lower basins of the Bhoj wetland. During the present study benthic fauna was represented by seven different classes belonging to Mollusca, Diptera, Trichoptera, Oligochaeta, Crustacea, Hirudinea and Ephemeroptera. Upper basin is richer in terms of species richness in comparison to the Lower basin. The dominance of Molluscan population in both the basins in terms of diversity and density has been recorded. Some benthic species also indicated the environmental stress in Upper basin of Bhoj Wetland on account of continuous deteriorating water quality. Physicochemical study revealed higher trophic status of Lower basin of Bhoj wetland in comparison to its Upper basin. Key words: Benthic population, Water quality, Trophic status, Bhoj Wetland. ORAL SECTION Page 10 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 USE OF PROTEINS ISOLATED FROM ADIANTUM AETHIOPICUM AS BACTERIAL AND FUNGAL AMYLASE INHIBTORS * Limaye A. S.; Bhosale K. S; Dushing Y. A. and S. L. Laware# Post-Graduate Department of Botany, Nowrosjee Wadia College, Pune-411 001 # Post-Graduate Department of Botany, Fergusson College, Pune-411 004 Fresh fronds of Adiantum aethiopicum L. were collected from local area around pune at various growth stages like a) Young fronds, b) mature fronds without sori, c) mature fronds with immature sori (pale green colour) and d) Mature fronds with mature sori stage (brown to black colour). They were extracted in 0.15 M NaCl and the clear supernatant was subjected to protein fractionation with ammonium sulfate. The fractions were dialyzed and tested for bacterial and fungal amylase inhibitory assay. Fractions between 31 % and 60 % saturation (F31-60) proved to have maximum activity for both the amylases; hence it was applied to a Sephadex G-100 column and eluted at a flow rate of 3.0 ml min-10. Eluted fractions were measured spectrophotometrically at 280 nm and tested for aforesaid assay. The elution number 6, 7, 8, 15, 16, 17, and 18 exhibited considerable activity. Maximum inhibitory activity (84.76%) for bacterial amylase and 78.36% for fungal amylase was observed in eluted fraction 8. The fraction is moderately thermo-stable as it is stable to the temperature ranging from 30 oC to 60 oC. The Maximum activity was recorded at 30oC and retained considerable activity up to 50 oC. It was also proved to be stable to different pH ranging from 3 to 9, but at pH 7.0 it showed highest inhibitory activity for both. Thus, the results of present investigation would suggest the possible use of proteins extracted from Adiantum aethiopicum L. as a part of defense mechanism against bacterial and fungal attack. Key words: Adiantum aethiopicum, proteins, Amylase Inhibitor (AI), Bacterial amylase, fungal amylase ORAL SECTION Page 11 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 Arbuscular Mycorrhizal Fungal (AMF) Dynamics along a field grown Linseed from Gondawale B.K. || Miss P. A. Mane, Dr. C. J. Khilare Shreemant Bhaiyyasaheb Rajemane Mahavidyalay, Mhaswad Dahiwadi college Dahiwadi An attempt was made to evaluate the quality and quantity of AM Fungi along a field grown Linseed from Gondawale B.K.II, Taluka-Man, District-Satara. AMF Spore number was found to increase with increase in age of the plants and even more in number in rizospheres.There was a variation in the number of AMF spores in rhizospheric soil of plants from different locations of field. The plants were found to be infested with various genus/species of AM Fungi. Maximum number of AM Fungal sporeswere recovered from rizosphericsoil of plants(235/100 gm Soil). In general six genera of AM Fungi and two unidentified AMF were found. Among all of these Glomus species were most common in the rhizosphere soil of Linseed in the fields. Entomopathogenic Fungi as Microbial Biocontrol Agent – A safe solution Meraj-ul-Haque and P.B. Nandkar Department of Botany RTM Nagpur University Nagpur merajmirage2000@gmail.com, pbnandkar@rediffmail.com Bioassays were conducted using the Entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae against insects and ticks of veterinary importance. Isolation of theses fungi was done from dead larvae of Musca domestica and Boophilus ticks. Surface and mass culturing of both the fungi were done in modified Sabouraud Dextrose Agar and Broth medium, respectively. Cultural characters of the fungi were studied. Surface culture of fungus Beauveria bassiana yielded white or lightly coloured colonies, whereas M. anisopliae produced herbage green or olivaceous green colonies. Mass culturing of fungi using other easily available field sources was discussed. ORAL SECTION Page 12 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 Efficacy of indigenous arbuscular mycorrhizal fungal inoculation on growth and nutrient uptake in Sorghum vulgare (Pers.) cv. Yeshoda D.D.Namdas and C.J.Khilare Department of Botany, Dahiwadi College Dahiwadi (M.S.) 415 508, India. ddnamdas@yahoo.co.in, cjk_principal@rediffmial.com A study has been undertaken to evaluate application of AM inoculants on growth enhancement in Sorghum vulgare (Pers.) cv. Yesoda at Ahmednager (Maharashtara). Plants were raised in sterilized soil in earthen pots and inoculated with 250g of soil containing 180-200 spores of pure of Glomus mosseae, G. fasciculatum, G. mosseae+G. fasciculatum and MykoVAM). All the plants in general responded well towards AM inoculation over control. Results obtained highlights plants inoculated with Glomus fasciculatum shown improved growth and nutrient uptake as compared with non-inoculated plants. The growth parameters such as plant height, fresh weight, dry weight, root colonization, nitrogen and phosphorous uptake were found increased due to influence of AMF inoculum over control plants. Furthermore, there was significant increase in percent mycorrhizal root colonization. The improvement in plant growth could be attributed to the enhancement of the plant to absorb more nutrients via an increase in the absorbing surface area. “ALLELOPATHIC EFFECT OF LEAF EXTRACT OF ACACIA NILOTICA L. ON GERMINATION IN JOWAR (SORGHUM VULGARE PERS. NOM. ILLEG)”. Pachangane P. M, Bhosale R.S, * Khomane B. V Department of Botany, Sharadchandra Pawar Mahavidyalaya, Lonand. Aqueous extracts of leaves of Acacia nilotica L. was applied on seeds of Jowar (Sorghum vulgare Pers. nom. illeg) to investigate their effect on percent germination, seedling height and seedling injury. Results observed at different conc. ranging from 10% to 100 % revealed gradual decrease in germination percentage and seedling height and increase in seedling injury with increasing conc. respectively. From present investigation it is clear that Acacia nilotica L. has an negative allelopathic effect on Jowar. ORAL SECTION Page 13 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 Invasion of weeds interrupting local biodiversity of Baramati Tahasil, Dist-Pune. Patil H S, Avchar B K, and Jagtap D K Department of Botany, Vidya Pratishthan’s Arts, Science and Commerce College Vidyanagari, Baramati, Dist- Pune. nisarghs@rediffmail.com The invasive weeds infesting the crop fields and other ecosystems have become a serious problem today. The agro ecosystems show association of various types of native and invasive weeds in crops and barren lands as well as waste lands. The studies on weed floristic indicated that more than 314 weed species are growing in Baramati Tahsil in different habitats. Amongst these dicot weeds were major and represented by 225 species, while the monocot weeds were represented by 89 species. The results on weed floristic clearly revealed that Gamopetalae was top most with 81 genera and 90 species of weeds. The native and invasive weed composition of Baramati Tahsil had given a clear cut picture of biological invasion with invasive weeds representing 10% of weed flora. Keywords: Invasive weeds, Baramati. HISTOCHEMICAL STUDIES ON ANTHERS OF AEGICERAS CORNICULATA Blanco. S. D. PATIL AND * D. R. SHIRKE. Department of Botany, Kisan Veer Mahavidyalya,Wai. Department of Botany, University of Pune. Histochemical localization of polysaccharides, proteins and nucleic acids have been done in anthers of Aegiceras corniculata Blanco. at successive growth stages. Tapetum, pollen mother cells and pollen grains are found to be rich in the metabolites. Wall layers show richness in polysaccharides, whereas other metabolites are in low quantity especially as anther approaches to maturity. Key words:- Histochemistry , anthers, Aegiceras , metabolites, tapetum, pollen grains. ORAL SECTION Page 14 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 Bioaccumulation of heavy metals by Hydrilla vercillata and increased levels of lead contamination by Typha latifolia Shankarao A. Patil, Madhavi A. Korane, *Smita V. Patil, Pooja A. Salokhe Department of Biotechnology, Vivekanand College, Kolhapur Aquatic plants play an important role in balancing water bodies. They are capable of acquiring large quantities of heavy metals that enters surface and ground water in various ways and adversely affects flora and fauna. Under present investigation Hydrilla vercillata and Typha latifolia young cattails, have been tested for removal of Iron,Copper,Nickel and Lead from metal solution. These plant have performed extremely well in removing Fe, Cu and Ni from their solution and were capable of removing Fe, Cu and Ni in large amounts in Hydrilla vercillata than Typha latifolia during 10 days analysis by AAS. The effectiveness of using both plants for phytoremediation of increased level of Pb contamination in soil, were harvested and separated into shoot, rhizome and root for analysis of Pb concentration in these tissues using AAS. Results shows that Hydrilla vercillata can remove Pb from environment in larger quantities at 1000 mg Pb/kg concentration whereas in Typha latifolia largest accumulation of Pb occurred at 4000 mg Pb/kg soil contamination level with shoots accumulating a combined average of 4744.03mg Pb/kg. These results show that three cattails can remove Pb from environment in larger amount. Further research should consider increasing quantity of cattails to optimize Pb removal from soil. Keywords : Aquatic plants, Heavy metals, Phytoremediation, Lead, AAS – Atomic Absorption Spectrophotometer ORAL SECTION Page 15 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 Petiolar Anatomy of Some Hitherto Unstudied Acanthaceae *Patil, A.M. and D.A.Patil Department of Botany, Rani Laxmibai College, Parola-425111, P.G. Department of Botany, S.S.V.P.S’s L.K.Dr.P.R.Ghogrey Science College, Dhule - 424005 (Maharashtra, India) patilajay231@gmail.com, dapatil_10aug@yahoo.co The present paper includes information on petiolar anatomy of hitherto uninvestigated 09 species belonging to 06 genera of the family Acanthaceae. The petioles (in T. S.) are usually circular or broadly circular in outline and adaxially channeled. The extent of development of endomorphic features and their distribution, inclusive of cell inclusions, are described. The vasculature is generally resolved into a central prominent arc alongwith few or none vascular bundles. The characteristics of epidermis, wings, pattern of vascular supply and sclerenchyma encircling it, cell inclusions, etc. are taxonomically significant and hence found useful for their identification. Keywords : Petiolar anatomy, Taxonomy. ESSENTIAL OIL COMPOSITION OF TRACHYSPERMUM AMMI (L.) FRUITS CULTIVATED IN PUNE (MAHARASHTRA) *Yogita Rohamare, T.D.Nikam and K.N.Dhumal Department of Botany, University of Pune Pune - 411007 (M.S.) India. E-mail: yogitaskandekar@gmail.com The demand and price of herbal products and essential oils are increasing consistently in the national and international markets, as over three-quarters of the world population relies mainly on plants and plant extracts for health care. The cultivation and processing of essential oil bearing and medicinal plants form an important area in the international agri-business. The popularity of Ajowan ((Trachyspermum ammi L.) is increasing due to its importance in seed ORAL SECTION Page 16 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 spices and medicines. The essential oil of Ajowan is amongst the most traded essential oil. The quality of ajowan oil depends upon the percentage of thymol present in it. Ajowan, is an herbaceous plant mainly grown for its fruits as a commercial spice crop in India, Iran and Egypt. The fruits find its use as an anti-spasmodic, anti- rheumatic, antiflatulent, diuretic and antimicrobial. The authentic seeds were sown in field during rabi season in the first week of December and harvested in the first week of May followed by using standard cultivation practices. The harvested seeds were air dried and kept in sealed platic bags before analysis. The essential oil of Ajowan (100 gm) seeds was isolated by hydrodistillation using Clevenger apparatus and stored at 40C before analysis. The essential oil was subjected to GC and GC-MS. The seeds possessed essential oil about 3.45% v/w. Twenty two compounds were identified of which thymol (42.44%) was present in greatest quantity. Other major components were pcymene (28.13%) and γ-terpinene (26.39%). Key words: ajowan, essential oil composition, thymol, p-cymene, γ-terpinene. Degradation kinetics and sorption study of kresoxim methyl on four different soils Rupali Sabale, Kaushik Banerjee, Soma Dasgupta, Sagar Utture, Dasharath Oulkar, Pandurang Adsule National Research Centre for Grapes, P O Manjri Farm, Pune 412 307, India The rate of degradation and sorption behaviour of kresoxim methyl was explored in 4 different soils of India. In all the test soils, namely clay, sandy-loam and loamy sand, the degradation rate was faster at the beginning, which slowed down with time, indicating a nonlinear pattern of degradation. Degradation in all soils followed 1st+1st order kinetics with halflife ranging between 1-5 days. The results suggest that organic matter might be playing a major role in influencing the rate of degradation of kresoxim methyl in soil. Rate of degradation in clay soil was fastest followed by sandy loam and loamy sand soils respectively. Comparison of rate of degradation in natural against sterilized soils suggests that microbial degradation might be the major pathway of residue dissipation. Adsorption of kresoxim methyl on studied soils ORAL SECTION Page 17 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 reached equilibrium within 48 hours. The relative adsorptivity of the test soils could be attributed to different organic matter and clay content of the soils. The data for rate constants, activation energies, enthalpy of activation, entropy of activation and free energy indicated chemical adsorption of kresoxim methyl on soil. The groundwater ubiquity score for different soils varied between 0 and 2.26, indicating kresoxim methyl as a chemical having low leaching potential. Keywords: Kresoxim methyl; soil; adsorption kinetic and isotherm; desorption; degradation rate kinetics; half-life. Screening of freshwater green algae Enteromorpha intermedia for its antimicrobial activity S. S. Sagar* sssbotany.2013@gmail.com *Department of Botany, Yashavantrao Chavan Institute of Science, Satara – 415 001 Freshwater green algae Enteromorpha intermedia collected from Godavari River at Kopergaon, Maharashtra was screened for its antibacterial and antifungal activities. The algal extracts were prepared in different solvents viz. alcohol, acetone, petroleum ether and chloroform, were tested for their antibacterial activity against Staphylococcus aureus and Escherichia coli. Algal extract prepared for sceeening antifungal activity was tested against the fungal pathogen Aspergillus niger. In the present investigation, the alga did not show any antibacterial activity against the tested bacteria but showed remarkable antifungal activity against A. niger. This study reveals that the fresh water alga E. intermedia can be a prospective phytoremedial tool against A. niger, the most commonly affecting fungal pathogen. Keywords: Godavari River, Enteromorpha intermedia, Antibacterial, Antifungal, Phytoremedial tool, Aspergillus niger. ORAL SECTION Page 18 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 In situ biodegradation of pesticides and food safety Varsha P. Salunkhe*, Indu S. Sawant, Kaushik Banerjee, Yogita R. Rajguru, Pallavi N. Wadkar, Dashrath P. Oulkar, S. D. Sawant National Research Centre for Grapes, Manjri Farm Post, Pune-Solapur Road, Pune 412 307, Maharashtra, India In the tropical humid climate of India, the fruits and vegetable crops are attacked by a number of insect pests and pathogens.For commercial cultivation farmers are forced to apply chemical pesticides to minimize losses. A large number of pesticides are registered in India with the CIB, Faridabad, specifying the crop, disease / pest, dosage and waitingperiod, in days, fromlast applicationtoharvest (http://cibrc.nic.in/mup.htm). This information is also provided by the manufacturers as label claims. Under GAP (Good Agricultural Practices) pesticides should be used in accordance to these claims to ensure food safety and freedom from pesticide residues above the prescribed minimum residue levels (MRLs). However, sometimes residues above the MRLs are detected in fruits and vegetables at harvest.Many of these pesticides are persistent and toxic in nature; hence detoxification of the produce is essential before they are consumed. One such method of detoxification is exploitation of microorganisms for the biodegradation of the pesticides to non-toxic metabolites. In this paper we present the possibilities of biodegradation of the organophosphorus pesticide, profenofos,on grape berries and vineyard soil, using Bacillus strains isolated from diverse habitats. ORAL SECTION Page 19 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 INFLUENCE OF AGROBACTERIUM RHIZOGENES ON INDUCTION OF HAIRY ROOTS FOR ENHANCED PRODUCTION OF WITHAFERIN A IN WITHANIA SOMNIFERA L. PLANT Parul Saxena*, Seema Ahlawat and Malik Zainul Abdin Centre for Transgenic Plant Development, Department of Biotechnology, Faculty of Science, Jamia Hamdard, New Delhi 110062, India. Roots, leaves and preparations of Withania somnifera (Ashwagandha) are traditionally used as tonic (the plant is sometimes referred as Indian ginseng), hypnotic, sedative and diuretic. The commercial cultivation of Withania somnifera has many problems like plant to plant quality variation, variation in quantity of active constituents, biosynthetic, morphological and genetic variability, long gestation period between planting and harvesting. Withanolides are high value chemicals and as the awareness towards health is increasing; its demand is also increasing continuously. Withanolide production from plant tissue cultures and induction of hairy roots in vitro have been considered to be a promising alternative, which offer a high degree of genetic stability, rapid growth and high profile of secondary metabolites than wild type plants. Hairy root cultures were developed from infection of different explants of in vitro germinated Withania somnifera L. plant with different strains of Agrobacterium rhizogenes. Hairy root line induced with LBA 9402 strain were selected on the basis of high Withaferin A content and growth. Integration of the TL-DNA (rol gene) region of the pRi plasmid was confirmed by polymerase chain reaction (PCR) analysis of the gene located in this region. The effect of different environmental factors like temperature, pH, cultivation media and carbon source on growth and Withaferin A production was studied in shake flask cultures. Maximum volumetric productivity of 1.4 mg L-1 day-1 was obtained in hairy root cultures. Key words: Agrobacterium, Withania somnifera L., Withaferin A, hairy root cultures. ORAL SECTION Page 20 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 Three Interesting Aero Aquatic Fungi From Maharashtra. Vaishali Shinde and *A.B. Pawar Department of Botany, Y.C. Institute of Science Satara- 415001. (M.S.) India During an investigation of aquatic fungi from various aquatic bodies located in different regions from Western Ghats in Maharashtra two new aquatic fungal genus viz. Candelabrum Beverw. Antonie van Leeuwenhoek and Cancellidium Tubaki have been described and illustrated. Candelabrum brocchiatum Tubaki and Candelabrum microsporum R.F. Castañeda & W.B. Kendr., were recorded on submerged decaying wood samples and twigs, and Cancellidium applanatum Tubaki was recorded on submerged stem and leaves of Eucalyptus globules Labill. These fungi are new records from this region. These results contribute to knowledge about the geographic distribution of freshwater hyphomycetes. These are new records to the fungal biodiversity of India. Key words: aero aquatic fungi, Candelabrum, Cancellidium. Effect of Boron toxicity on seed germination in three Hibiscus species *Sonar, B. A and Chavan, P. D. Department of Botany, Shivaji University, Kolhapur-416 004 (MS) INDIA. baburao.2007sonar@gmail.com Influence of boron toxicity on seed germination, seedling growth and activities of two antioxidant enzymes in three Hibiscus species namely, Hibiscus cannabinus L., Hibiscus sabdariffa L. and Hibiscus tiliaceus L. was investigated. Germination percentage of all the three Hibiscus species was decreased due to higher ‘B’ concentration (200 ppm ‘B’). Higher dose of boron (200 ppm ‘B’) caused marked reduction in germination percentage in H. tiliaceus (86.67 %) and H. cannabinus (20 %) while, in case of H. sabdariffa (2.18 %) decline in germination percentage was significant. Root length of all the three Hibiscus species was ORAL SECTION Page 21 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 declined due to higher concentration of boron (200 ppm ‘B’). Root length of the three Hibiscus species affected under ‘B’ stress in the order H. cannabinus > H. sabdariffa > H. tiliaceus. Shoot length also similarly reduced due to 200 ppm ‘B’ concentration but the order of decrease was H. tiliaceus > H. sabdariffa > H. cannabinus. Higher dose (200 ppm ‘B’) of boron concentration reduced the total length of all three Hibiscus seedlings. The percent reduction in seedling length at 200 ppm ‘B’ concentration was in the order H. tiliaceus > H. sabdariffa > H. cannabinus. Considerable reduction in vigour index of all the three Hibiscus species was noticed in response to 200 ppm ‘B’ treatment in following order H. tiliaceus > H. cannabinus > H. sabdariffa. Decrease in fresh weight per seedling of H. cannabinus and H. sabdariffa was noticed under ‘B’ toxicity while, in H. tiliaceus there was slight increase in fresh weight at 100 ppm ‘B’ concentration and there after it was decreased. Catalase and peroxidase activities in seedlings of H. sabdariffa were greatly enhanced due to 150 ppm and 200 ppm ‘B’ treatment respectively. Lower dose (100 ppm ‘B’) caused slight increase in catalase activity of both H. cannabinus and H. tiliaceus seedlings. The activity of enzyme peroxidase was reduced in seedlings of H. cannabinus and H. tiliaceus over entire range of ‘B’ treatment. Thus an interspecific difference in boron tolerance at germination stage was evident in the three Hibiscus species in the present investigation. Key words: Germination, Hibiscus, Boron toxicity. ORAL SECTION Page 22 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY JANUARY 18th - 19th, 2013 POSTER ABSTRACTS NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 ANTINUTRITIONAL ACTIVITY IN SOME PTERIDOPHYTIC PLANTS Ahiwale N.H., Savant S.H., Patil A.S. and Patil R. B DEPARTMENT OF BOTANY Y.C.I.S, SATARA. nita.biodiversity@gmail.com Pteridophytes are the most primitive vascular plants, found in living and fossil conditions. Generally animal avoids feeding on pteridophytic plants, it indicate that these plants may have any kind of toxicity. Antinutritionals are natural or synthetic substances which adversely affect health and growth of human and animal both. In present investigation three pteridophytic plants viz Pteridium aquilinum, Adiantum incisum, Cyathea dealbata were studied for their antinutrional contents. It is evidence from result that the highest level of tannins and nitrate found in Cyathea dealbata. Key Words - Antinutritional, pteridophytes, tannin, nitrate. BRYOFLORA OF KAS PLATEAU AND ADJOINING AREA S. A. BAGWAN AND B. A. KORE Department of Botany, Yashvantrao Chavan Institute of Science, Satara – 415001 basavaraj.kore@rediffmail.com 36 species of bryophytes comprising 18 liverworts, 16 mosses and 2 hornworts are being reported first time from Kas plateau and adjoining area. Some of the noteworthy species are Riccia crystallina L., Cryptometrium himalayense Kash., Porella platyphylla L., Macromitrium sulcatum (Hook.) Brid, pinnatella calcuttensis Fleisch., Hypophila involuta (Hook.) Jaeg, Hydrogonium consanguineum (Thwait et Mitt.) Hilp. which need to be conserved against anthropogenic pressure. Keywords: Bryophytes, Kas plateau and adjoining area, anthropogenic pressure POSTER SECTION Page 23 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 Cythodium tuberosum, Kash. (Liverwort)- A new host of Eudonia mercurella. Bagwan, S.A. and B. A. Kore Department of Botany, Y.C.I.S., Satara 415001 (MS), India. basavaraj.kore@rediffmail.com During routine survey of bryophyte diversity of hills around Satara for the last two years caterpillar feeding on Cythodium tuberosum was observed in rainy season. The life cycle stages were rared in the laboratory and the insect was identified as Eudonia mercurella. It is a moth belonging to Family Crambidae & order- Lepidoptera. The caterpillar was just feeding on Cythodium tuberosum & had not attained a pest status. Literature survey states that this insect also feeds on Mosses & Lichens but there were no reports on Liverwort viz. Cythodium tuberosum. ORGANIC CULTIVATION OF STRAWBERRY THROUGH APPLICATION OF FERN FROND BIOMASS *E.B. Bhalerao and K.N. Dhumal *Department of Batany, Kisan Veer Mahavidyalaya, Wai. Department of Botany, University of Pune, Pune-411007. bhaleraoek@gmail.com Mahabaleshwar is the most important hot spot of rich biodiversity in the western Ghat. Different types of fern species like Tectaria macrodonata and Pteridium aquilium are luxuriantly growing in this evergreen forest. These dominant ferns are producing enormous biomass throughout the year. This natural biomass was utilized in the form of frond extracts for foliar application of strawberry, Fragaria xannanasa, Cv. Sweet Charlie. It was found that these herbal extracts served natural plant growth regulators for Strawberry, which had enhanced the organic constituents like proteins, phenols total and reducing sugars, starch as well as photosynthetic pigments at very low concentrations. The fern frond extracts had also improved the fruit quality in terms of vitamins C, total soluble solids (TSS) and fruit yield. The findings of present investigation POSTER SECTION Page 24 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 have clearly indicated that the local strawberry growers can beneficially utilize the natural resources like fern biomass for organic and sustainable production of strawberry and other crops. This can become eco-friendly technology for different agricultural crops in future. Keywords: Organic farming T. macrodonata, P. aquilinum, biomolecules, Strawberry IN-VITRO MULTIPLICATION OF TYLOPHORA INDICA BURM. F. AN ENDANGERED HIGH VALUED MEDICINAL PLANT *G. P. Chaugule and *A. R. Nalwade Plant Tissue Culture Research Laboratory, Annasaheb Awate Arts and Hutatma Babu Genu Science College, Manchar. Dist. – Pune (MS) Simple, reproducible, efficient and rapid protocol has been developed for in vitro propagation of an endangered medicinal plant (TylophoraindicaBurm.f.) using axillary bud explants. Explant werecollected from young shoots of a three-year old plant from herbal garden of the college and werecultured on Murashige and Shoog (1962) medium augmented with increasing concentrations of 6-Benzylaminipurine. The optimum concentration of BAP for shoot multiplication was recorded at 3.0 mg/l. In-vitro grown shoots were transferred to MS medium containing increasing concentrations of Indole 3-butyric acid. Maximum number of roots was produced at 1.0 mg/l IBA. Plantlets were transplanted in a mixture of vermiculite and soilright (1:1) for hardening and hardened by keeping in polybags for first 20 days and then in the polyhouse for next 30 days. 90% success was achieved. The described method can be successfully employed for large-scale multiplication and long-term in-vitro conservation of this endangeredmedicinal plant. Keywords: Tylophoraindica, 6-Benzylaminopurine,,Indole 3-butyric acid, In-vitro multiplication, POSTER SECTION Page 25 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 Phytochemical analysis of some weeds Chavan Yogesh R., Thite Sachin V. Aparadh V. T. and Kore B. A. Department of Botany, Y.C.I.S., Satara 415001 (MS), india. chavanyrc88@gmail.com Weeds are unwanted plants growing at waste places as well as many other important places like farm yards, play grounds, public places, water bodies, etc. Weeds are well known for their negative importance but they are easily available in large amount. There are different ways to control weed population, one of them is their use for human betterment. Weeds are noxious & difficult to control, but few of them are having medicinal properties hence the qualitative phytochemical screening of some weeds viz; Alternanthera sessilis, Amaranthus spinosa, Lantana camera, Xanthium strumarium has been carried out. The phytochemical analysis of these weeds gives idea about secondary metabolites present in them such as Alkaloids, Cellulose, Carbohydrates (sugars), Flavonoides, Glycosides, Phenol, Quinon, Saponin, Tannins, Terpinoids, Triterpinoids, and Steroids. Keywords: Phytochemicals, weed, secondary metabolites, Alternanthera sessilis, Amaranthus spinosa, Lantana camera, Xanthium strumarium. Effect of salt & drought stress on two varieties of Capsicum annum grown in Maharashtra Gaikwad S. E. and Shinde R. A. Department of Botany, Yashavantrao Chavan Institute of Science, Satara An intensive problem in agriculture is salinity & drought which affects germination, seedling growth & yield of several crop species. Capsicum annum is a widely cultivated tropical crop for its fruits in Maharashtra, Karnataka, Andhra Pradesh and Gujarat. Fruits are used in condiments spices & many confectionaries. Fruits are an excellent source of various antioxidant POSTER SECTION Page 26 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 compounds like flavonoids, carotenoids, vitamin C and the active compound capsiacin .These compounds help protect human body against oxidative damage & prevent various diseases such as cancer & cardiovascular diseases. The objective of this study was to define the effect of salt and drought stress on two varieties of C. annum viz var. sitara (native to Maharashtra) and var. bedgi (introduced from Andhra Pradesh). Germination studies, nitrate reductase activity and free proline accumulation were investigated under lab conditions to compare the two varieties under salt and drought stress. Key words: Capsicum, salt, drought stress Protective effect of ethanolic extract of Lactuca sativa against D-galactose induced oxidative stress in silkworm Bombyx mori Gaikwad Y. B. and Bhawane G. P. Department of Zoology, Shivaji University, Kolhapur The antioxidant properties of ethanolic extract of Lactuca sativa was studied in silkworm Bombyx mori. The oxidative stress was induced by oral administration of D-galactose. The larvae were treated with ethanolic extract of L. sativa simultaneously with D-galactose and independently to analyze the antioxidant efficiency for three days in fourth and fifth instar. On fifth day of fifth instar lipofuscinogenesis was studied histologically in brain and spectroflurometrically in midgut tissue of silkworm B. mori. The results showed increase in lipofuscin granule density in brain and higher fluorescent products in midgut of D-galactose treated groups. There was decrease in both brain lipofuscin granule density and fluorescent products of midgut tissue in ethanolic extract of L. sativa treated groups showing free radical scavenging property of the extract. Key words: Lipofuscninogenesis, D-galactose, L. sativa and B. mori POSTER SECTION Page 27 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 FLORISTIC STUDY OF SHIRUR REGION (PUNE, MAHARASHTRA) Ravindra P Ganorkar* Department of Botany, C.T. Bora College Shirur ravindraganorkar@gmail.com The investigation was carried out in order to explore the existing floristic composition in Shirur, Pune (Maharashtra). The present area of Shirur, Pune is selected for the floristic studies because it has been given little attention of its vegetation. The vegetation was arid to semiarid and dry deciduous, thorny scrub type. Over 121 plants belonging to more than 42 families were studied. Among 42 families, Caesalpiniaceae and Mimosaceae are the most dominant family. Key words: floristic, studies, Shirur, vegetation. AN EFFICIENT REGENERATION AND MULTIPLICATION OF WITHANIA SOMNIFERA DUNAL,FROM NODAL EXPLANTS *Gawade B.B, Bolbhat S.N, Wadvkar D.S, Shendage V.S. Department of Botany,Dada Patil Mahavidyalaya,Karjat,Dist-Ahmednagar,PIN-414402. Withania somnifera Dunal is a medicinally important herb from family solanaceae.Therefore they attempts were made to develop efficient regeneration and multiplication of Withania somnifera Dunal from nodal explants.For efficient regeneration and multiplication from nodal explants in Withania somnifera Dunal the nodal explant were cultured on MS medium containing cytokinin BAP and kinetin alone and in combination at different concentrations. Among the two cytokinin kinetin showed better results than cytokinin BAP when incorporated alone at different concentrations ( 0.5 to 2.0mg /lit.) in MS medium. The maximum percentage of explants showing shoot formation and maximum number of shoots per explants was noticed on cytokinin kinetin at 2mg/lit.in MS medium than BAP 2mg/lit. in MS medium .The best result for efficient regeneration and multiplication of shoots POSTER SECTION Page 28 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 were obtained when nodal explants were cultured on MS medium containing cytokinin BAP and kinetin in combination. Maximum number of shoots and maximum percentage (100%) of explant showing shoot formation was noticed at MS+2mg/lit. in kinetin +0.5mg/lit BAP.When nodal explant cultured on MS medium containing combinations of cytokinin kinetin and BAP at different concentrations MS+0.5mg/lit.kinetin+2.0mg/lit.BAP,MS+1.0mg/lit.kinetin +0.25mg/lit. BAP,MS+2.0mg/lit.Kinetin+0.5mg/lit.BAP,among these media MS+2.0mg/lit.kinetin +0.5mg/lit. BAP,was best for shoot induction and proliferation in Withania somnifera Dunal. Keywords: Withania somnifera ,solanaceae,BAP,kinetin,explants,cytokinin.MS. BLUE GREEN ALGAE FROM KARAD, DISTRICT SATARA Sharda J. Ghadge* And Mrs. Vanita Karande S.G.M. College Karad* Post Graduate Department of Botany, Y.C.Institute of Science, Satara vaneetachandra@gmail.com Present communication deals with taxonomic enumeration of sixteen taxa of cyanophyceae belonging to 9 genera collected from different habitats situated in and around Karad. Algal samples were collected and preserved in 4% formalin. They were identified by using standard literature(Anagnostidis & Komarek 1988, Desikachary 1959). The genera identified were Microcystis, Chroococcus, Gloeocapsa, Microchete, Oscillatoria, Lyngbya, Microcoleus, Anabaena, Rivularia etc. Result indicated that Oscillatoria dominated the population of blue green algae from study area. Keywords: Blue green algae, taxonomy. POSTER SECTION Page 29 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 FUGAL DIVERSITYAT KADEGAON TOWN OF SANGLI DISTRICT (M.S.) INDIA. M.M. Ghatge*, V.S. Salunkhe*, R.R.Jadhav*, * Department Of Botany, Bharati vidyapeeth’s Matoshri Bayabai Shripatrao Kadam Kanya Mahavidyalaya Kadegaon.415304(M.S.), India manda.ghatge@rediffmail.com The present investigation deals with the study of aerospora at kadegaon. The observations were done during the two years. Study of aerospora of Kadegaon at different sites has been done. During the investigation the aerospora of Kadegaon in December to January shows some common fungal aerospora at particular period of appearance in the air. Dominating fungi are of Duteromycetes about 25 species, Phycomytes 6, Ascomycetes 12 and Basidiomycetes 5 spccies. Out of which some are pathogenic ,some are saprophytic , some fruit rotting while Aspergillus Micheli ex Link, Mucor Micheli ex.fr, Rhizopus Ehrenberg, Aspergillus Micheli ex Link are highly allergenic causing diseases to human being and plants also. Appearance of these particles is characteristic and metrological useful factor. By knowing the period and allergenic characteristic one can prepare a personal calendar to avoid allergenic diseases as well as metrological forecasting the weather conditions and to control the plant diseases. Key words:- Aerospora, pathogenic, Allergenic, metrological Phytochemical screening of various extracts of ‘Saptarangi’ (Salacia chinensis L) B. A. Ghorpade*, J. J. Chavan Department of Botany, Yashavantrao Chavan Institute of Science, Satara bhagyashrighorpade1@gmail.com Salacia chinensis L. (Family - Hippocrateaceae), commonly known ‘Saptarangi’ is distributed in tropical areas including India, Sri Lanka, southern China and other Southeast Asian countries. Economically, Saptarangi is of appreciable importance due to its wide use in traditional medicines, many pharmacological activities (including antidiabetic) and edible fruits. POSTER SECTION Page 30 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 Attempts have been made to find out phytoconstituents of different extracts of S. chinensis. The aqueous and alcoholic extracts of root, stem and leaves showed the presence of various phytoconstituents. All the tested phytochemicals were detected in aqueous as well as alcoholic extracts of all three parts viz. root, stem and leaves while flavonoids were not detected in both the solvents. Phytochemical analysis revealed the presence of carbohydrates, alkaloids, saponins, phytosterols, phenols, and tannins as the phytochemical components in various extracts of root, stem and leaves of S. chinensis. Keywords: Antidiabetic, Salacia chinensis, Phytochemical, Alkaloid, Tannin, Phenol. STUDY OF STOMATAL DIVERSITY IN SOME ANGIOSPERMIC FAMILIES Gole A.A., Ramugade M.A., Bhagwat H.R., Gaikwad S.S., Mohite P.C. and Aparadh V. T. Department of Botany, Yashavantrao Chavan Institute of Science, Satara ashagolemeet@gmail.com Here in current article 10 families representative 10 genera studied for their stomatal type. Parasitic stomata observed in most of the studied families.(viz.Asteraceae, Verbenaceae, Malvaceae, Commelinaceae, Acanthaceae, Rubiaceae, Euphorbiaceae, Rosaceae, Hameliaceae, Sapotaceae) It may indicated taxonomic relationship of these families. Also measurement of stomatal size for each families were carried out with the help of Olympus steriobinacular microscope using Dewinter Biowizard software unit of measurement . Keywords: Stomata, measurement tool, Angiosperm families POSTER SECTION Page 31 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 Preliminary Phytochemical Analysis of Some Gymnosperm species Gurav K. D., Thite S.V., Patil P. R., and Kore B.A. Department of Botany, Yashavantrao Chavan Institute of Science, Satara kuladipiyu1617@gmail.com It is a universal fact that always plants play an important role in human life. India is well known for its richest biodiversity. But in the present era plants like gymnosperms are ignored for their conservation. This paper deals with primary screening of some ornamental gymnosperms viz, Araucaria hetrophylla, Cycas circinalis, Thuja occidentalis and Zamia furfuracea for their wealth of phytoconstituents by using preliminary qualitative phytochemical tests. The fresh foliage tissue was obtained from botanical Yashwantrao Chavan Institute of Science College, Satara and phytochemical screening was performed as per standard methods for the presence of secondary metabolites (viz, Saponins, Phenols, Tannins, Flavonoids, Sugars, Triterpens, Phytosterols). Keywords: Gymnosperm, Secondary Metabolites, Phytochemicals Fungal biodiversity in seeds of some cereals. Magdum J. A. Dept. of Botany, Mahatma Phule Mahavidyalaya, Pimpri, Pune. 17 jayashri2501@yahoo.com Seeds samples of six different cereals were screened for ectophytic fungi. Qualitative and quantitative estimation of fungi associated with seed samples collected from different localities was carried out. 21 fungal species were found associated with seeds of six cereals. Minimum fungi were reported from seeds of Pennisetum typhoides and Sorgum vulgare. Aspergillus flavus, Fusarium moniliformae and Fusarium oxysporium were found pathogenic causing seed rot, seed discolorations and germination inhibition. Captan and Dithane M-45 proved best for cereals dressing. Keywords: Cereals, Fungal biodiversity, Fusarium oxysporium POSTER SECTION Page 32 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 NUTRITIONAL STATUS IN POMEGRANATE AND CUSTARD APPLE JADHAV A.R., KANASE R.M., KADAM D.A. Department of Botany of Y.C.I.S.satara. asjadhav2391@gmail.com Fruits are important for medicinal and edible purpose. In the present studies nutitional status of Annona squmosa and Punica granatum with respect to Carbohydrates, Protins, Amino acids, Polyphenols, Chlorophylls, Sucrose is detemnied and the results analysis is discussed. KEY WORD - Nutritional, pomegranate, custard apple, carbohydrates LIST OF FLOWERING PLANTS (DICOT) FROM KHAMBATAKI GHAT OF SATARA DISTRICT. *Damayanti Jadhav and G.N.Badave *Department of Botany, Rajendra Mahavidyalaya Khandala, Dist: Satara jadhavdamayanti@gmail.com; girishbadave@yahoo.com Khambataki Ghat (Tahasil- Khandala Dist- Satara) is situated on northern border of the district. It is the part of mountainous region of “Sahyadri” and located on Pune-Satara Highway Which is a part of National Highway No.4. It is located at the Latitude 18º3N and Longitude 74º01E and length of the ghat is about 6km.approx. Dicot flora of the region was studied in the month of November and list was prepared. Flora is represented by 42 families and 80 spp. Flora was dominated by families Fabaceae 10 spp., Asteraceae 9 spp., Acanthaceae by 4 spp., Euphorbiaceous, Mimoseae, Cucurbitaceae, Lamiaceae 3 spp.each, 9 families were represented by 2 spp. While 26 families represented by 1 spp.each. Dominent spp. were Securingea virosa (Roxb.wild), Scutia indica Brongn , Vernonia divergens Edgew, Woodfordia fruticosa (L), Senecio edgeworthii Hook, Rhus mysurensis (L), Pogostemon paniculata Benth. Dominent Climbers were Clematis triloba, Cocculus villosus. Dominent trees are observed were Boswellia serrata Roxb. Bridelia retusa, Acacia leucophloea wild, Azadiracta indica A.juss, Tamarindus indica L., Ailanthus excelsa Roxb, Ficus spp. And Odina woodier Roxb. Succulent and Xerophytic plants represented were Notonia grandiflora POSTER SECTION Page 33 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 DC, Opuntia spp, Sarcastema brevistigma wight, Capparis divaricata Lam, and herbs like Tribulus terrestris L., Acanthospermum hispidum L. Glyrcidia was introduced by forest in the area during reforestration programme. The only endemic plants observed in the survey were Barleria lawii T. Anders of family Acanthaceae. Edible fruits collected were Annona squamosa, Rhus mysurensis (L), Tamarindus indica L. Carrisa congesta wight etc.Vegetation was of dry deciduous type. MEDICINAL HERBS, BEING USED AS NATURAL HEALERS, FOR FUNGAL INFECTION Jadhav S.N*, Kadam D.A.**, Kadam. Abhijeet,*** Jakhi.P.S.**** P.G.department Botany Yashwantrao Chavan institute of Science Satara. **** Institute of Science Nagpur. Phama a deuteromycetes fungus inhibiring soil, is also known as parasite on certain plants. It was found to infect leaves of adathoda vasica a common medicinal plant. It is used in traditional medicine for symptoms of common cold cough, inflammation and manyother disorders. Under these pathological conditions,the damaging factors are the generation of the reactive oxygen species and other free radicals. The herbal prepration like ‘vasa’ prescribed in the pathologies, therefore must have high antioxidant potency themselves and also have a capacity to strengthen the anitionxidant defense of the patients consuming them. Objectiveof the present study was to find out undesirable effects, if any of the fungal infection of this platn. For this healthy leaves of Adathoda vasica leaves infested by phoma sp and biomass of the fungus was asertained in vitro. The parameters used for this were the free radical scavenging capacity, iron chelating activity and reducing power along with phenolic and flavonoid contents. It was observed that reducing power as well as flavonoid and phenolic contents of the fungal infected herb was lower than that of the healthy herb. The fungal biomass was seen to lack free radical scavenging capacity. POSTER SECTION Page 34 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 Iron chelating acitivity reducing power, and had nigligble Phenolic and flavonoid content. It was thus inferred that the change in antitoxiant potential of the fungal infested herb is due to the physiological changes in the herb brought about by the pathogen. This finding substantiales the need to screen the medicinal herb for the infestations by pathogens. Key words : Phoma, Adathoda vasica, antitoxidant Weed Celosia argentea linn. Used us Ayurvedic medicine By rural people from Satara Taluka (ms). Jadhav S.N*, Kadam D.A.**, Kadam. Abhijeet,*** Jakhi.P.S.**** P.G.department Botany Yashwantrao Chavan institute of Science Satara. **** Institute of Science Nagpur. Celosia argentea Linn is a vigorously growing annual weed belonging to the Amranthaccae family. It is commonly known as quailgrass, feather cockscomb, soko, kurdu, kombda and murga kalgi. it is native tropical Asia. Now it is widely spread across north America, Africa, West indies. It grows in temprate as well as tropical regions. It is vigorously growing in Non irrigated area from Satara Taluka village like Rajewadi, Degaon, Nigadi, Varne, Bharatgaon, Nagthane; Because of flavour ,food value and familiarity the leaves, thender,shoots, and seeds are widely consumed in several people of these village. It is also used as forage for poultry Birds and buffalos. Despite being it is widely it is widly used as medicine. It is used in the treatment of colic,cough,diabetes mellitus, diarrhoea, dysentery, eye problems, gonorrhoea,liver ailments, menstruation problems, muscle trouble, Snake bites,wounds and in intareatment of intestinal tapeworm. The leaves are employed as dressings for boils and sores. The roots have diuretic POSTER SECTION Page 35 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 properties. Seeds have antipyretic, antisplasmodic, antibacterial, property and also useful in Jaunrice. Therefore detailed pharmacognostic study has been carried out and presented in this paper. Mostly rural people said that it is weed but these people used this seed as medicine. It is can be further useful in the prepration of medicine in various systems of medicine such as Ayurveda Siddha, Unani, Tibbi and Amchi. Key words : Celosia, argentca, diarrhea, Rajewadi, Unani. SACRED GROVES A MINOR FOREST PRODUCT RESERVE FROM SATARA DISTRICT.(MS) Jadhav S.N*., Jakhi P.S.**, Irannawadi Rehuka*** P.G.department Botany Yashwantrao Chavan institute of Science Satara. ** Department of Botany Insititute of Science, Nagapur. *** U.H.D.E. India Ltd. Environment department Bebawewadi, Pune. Sacred Grove is a patch of forest land which will be preserved and unharmed having religious sentiments attached to it. It is traditional method of in- situ conservation. Since the assessment of the sacred groves of satara district for its potential for minor forest product had been worked out of some places from satara district. A study on this aspect has been presented in this paper. A study of the floristic composition and interaction with local people is also incorporated. In the present paper an account of MFP’s viz medicinal, edible, fodder species, aromatic, veterinary medicine, fiber yielding, one for tan for dyc, for gum, for resin, some plants for making plates, dead seeds, spices, for fish poison, threatened, endemic plants reported in this paper. In present paper sacred groves from pateshwar, Malaidevi, jarandeshwar, sajjangad, mandhardevi, kinai, vardangad, Aundh yamai, walmiki, nandgiri all thes places contain tamples of god and godess form these places Tectona Terminalia speeies were common. The traditional POSTER SECTION Page 36 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 conservation practice is endangered these plants from sacred groves protected by Bhagats present in tamples in these horest. But rural peoples from these area due to the demand of the minor forest product the depletion of the plants was on rise. Meany of the rare and endangered plants would be extinct. Key words:- Sacred grove, Bhagats, Pateshwar, Endengered. SURVIVAL ABILITY OF CARBENDAZIM RESISTANT ISOLATE OF SCLEROTIUM ROLFSII CAUSING RHIZOME ROT OF TURMERIC A. A. Jagtap, M. S. Desai, M. N. Walavade, S. P. Dorugade, Y. S. Andoji and S.S. Kamble Department of Botany, Shivaji University, Kolhapur Survival of carbendazim isolate of Sclerotium rolfsii among the sensitive population was studied on the turmeric rhizomes. On the untreated rhizome resistant population in the mixture was reduced from passage to passage. But on the carbendazim treated turmeric rhizomes resistant population was increased from passage to passage. Keywords- Carbendazim, Turmeric, Sclerotium rolfsii. SEED GERMINATION OF PEDALIUM MUREX L. A.M. Jamadar, S.K. Khade and V.C. Karande* Dattajirao Kadam Arts, Science and Commerce College, Ichalkaranji Tal. Hatkangale, Dist. Kolhapur. Pin.- 415116. *Yashvantrao Chavan Institute of Science, Satara. jafsana@gmail.com Pedalium murex L. is a member of Family Pedaliaceae. It is widely distributed in sea coastal region of India. It is an important medicinal plant and it has been used traditionally in various disorders and also as a health tonic. Present study deals with seed germination of Bada POSTER SECTION Page 37 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 gokharu were effective in storage periods up to 9 to 10 months. Germination also increased with scarification method, and maximum germination was observed at the temperature range of 100C - 300C. However, conc. acids were not found useful in seed germination. Key Words: Germination, Scarification, Temperature, Acids, Pedalium murex L. RESPONCES OF PLECTRANTHUS BARBATUS (ANDR.) SYN. COLEUS FORSKOHLII (WILLD.) TO FOLIAR APPLICATION OF CCC (CHLORO CHOLIN CHLORIDE). Joshi. A. S. and Chavan P. D. Department of Botany, Shivaji University, Kolhapur. (M. S.) INDIA asmi_jasmine82@yahoo.com An attempt has been made to study the effect of foliar application of CCC (Chloro Choline Chloride) (100 and 500ppm) on mineral nutrition status of local variety of Coleus forskohlii which is an important medicinal plant having tuberous roots containing , a diterpene forskolin. The plant is widely used as an antiallergic, antiasthmatic, antiglaucomatic, antihypertensive agent and against skin diseases. Foliar sprays of CCC have altered the mineral nutrient status in all plant parts. The amounts of Nitrogen and Phosphorus were reduced in all plant parts due to both doses of CCC. The accumulation of Calcium was increased in leaf and stem while opposite trend was found in accumulation of Magnesium in case of leaf and root by both treatments. Potassium accumulation was found to be elevated in root, stem and leaves except in leaves of plants treated with low dose of CCC and that of Iron and Manganese was decreased in leaf and stem as a result of both treatments. Both the applications of CCC lowered the accumulation of Zink in all plant parts but Copper content was raised in root and stem. Keywords - Coleus, CCC, mineral POSTER SECTION Page 38 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 Diversity and Photosynthetic Pigments Dr. P. S. Kadam Prof. Ramkrishna More ACS College, Akurdi, Pune-411044 Malavali, around 65 kms from Pune, is a popular destination that is well known for its rich biodiversity. In the present study, several field trips were carried to this place during AugOct, 2012, and the Pteridophytes, namely, Adiantum, Chielanthus, Nephrolepis, Pleopeltis and Pteris grown in this area were collected every month and identified. Studies on phytosynthetic pigments, i.e., chlorophylls and carotenoids and the total polyphenols were carried out. It was found that there was decrease in the total chlorophylls, except in Pleopeltis, and the carotenoid content also was observed to be decreased. The total polyphenols also decreased in all the Pteridophytes studied. This decrease can be attributed to the changing environmental conditions caused by increase in pollution due to tourists visiting this place. Hence, conservation of the rich biodiversity, which is depleting, is a needful priority. Keywords: Diversity, Pteridophytes, Photosynthetic Pigments, Chlorophylls, Carotenoids Evaluation of total phenolic content in different parts of Solanum xanthocarpum A. P. Kadam*, N. B. Salunkhe, V. T. Aparadh, D. A. Kadam, J. J. Chavan Department of Botany, Yashavantrao Chavan Institute of Science, Satara – 415 001 amrutakadam9@gmail.com Solanum xanthocarpum Schrad. & Wendl. (Family – Solanaceae), commonly known as yellow berried nightshade, is a perennial herb distributed throughout India, mostly found in dry places and wastelands. The plant is known for several traditional medicinal uses including anthelmintic, wound healing, antipyretic, laxative, anti-inflammatory, urinary bladder, and anthiasthmatic activities. The goal of the present work is to optimize the extracting solvent for obtaining total phenolic contents from root, stem, leaf and fruits of S. xanthocarpum. Ethanolic fruit extract exhibited the highest phenolic content (16.72±1.0 mg/100 g DW) whereas; stem extract in the same solvent showed lowest amount of phenolic content (0.66±0.3 mg/100 g POSTER SECTION Page 39 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 DW). The findings of present study revealed that ethanol is the best solvent for extraction of total phenolics and fruits of S. xanthocarpum are very good natural source of phenolics for its use in pharmaceutical industry. Keywords: Solanum xanthocarpum, Total phenolic content, Solvent, Optimization. Preliminary Investigation of secondary metabolites from viral diseases Kadam P. K.., Dhumal N. C., Dhumal T. C., Barge A. N., Y. A. Ghule and H. L. Shinde. Department of Botany, Y.C.I.S.Satara – 415001, Maharashtra, India uraj84@gmail.com This paper deals with Phytochemical studies in two virally infected plant i.e Chilli and Agitata Phytochemical tests were carried out to know about the qualitative existence of secondary metabolites in them. For the current article screening of alkaloids, anthocyanins, cellulose, flavonoids, glycosides, leucoanthocyanins, phenols, quinones, saponins, tannins, terpenoids, and have been carried out. Preliminary studies of the antifungal activities of some medicinal plants against Aureobasidium pullulans Kadam, P. A., Vaishali Shinde, Chavan, Y.R, and Pankaj Mundada Department of Botany, Y.C.I.S.Satara – 415001, Maharashtra, India The antifungal activities of extracts of 5 medicinal plants collected from Satara were tested against two pathogenic fungi using agar plate methods. This study was carried out with an objective to investigate the antifungal potentials of leaves of Adhatoda zeylanica Linn., Lantana camara Linn., Cynodon dactylon(L.) Pers., Azadirachta indica A. Juss. and Ocimum sanctum Linn. The aim of the study is to assess the antifungal activity of extracts on Aureobasidium pullulans fungal strain. A series of 5 Indian medicinal plants belonging to 5 POSTER SECTION Page 40 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 different families used in various infectious disorders, were screened for their antifungal properties. Nearly all the extracts were found effective against these fungi. Keywords: Medicinal plants, Antifungal activities, Aureobasidium pullulans. ALLELOPATHIC EFFECT OF ARAUCARIA AND THUJA ON WHEAT AND JAWAR GERMINATION AND GROWTH Nilam Kamble*, Jyoti Shinde, Rohini Shinde Department of Botany Yashavantrao Chavan Institute of Science, Satara nilam.kamble@gmail.com The paper deals with the study of Allelopathic effect of Araucaria and Thuja on Wheat and Jawar under laboratory conditions. The extracts of Araucaria and Thuja were prepared as 5%, 10%, 15%, 20% concentrations in distilled water. Allelopathy offers potential for biorational weed control through production and release of allelochemicals from leaves, flowers, seeds, stems and roots of living or decomposing plant materials. Under appropriate conditions, allelochemicals may release in quantity which suppress the developing weed seedlings and often exhibit selectivity. The suppression of weeds by crops should be exploited to improve weed control. The effect of Araucaria and Thuja was evaluated by determining the germination percentage of seeds, length of root, shoot as well as seedling and germination rate. The allelopathic potential of the Araucaria and Thuja was confirmed for the highest doses tested (5, 10, 15, 20 % of the extracts). In conclusion, it shows a potential allelopathic effect of Araucaria and Thuja extracts which inhibits the growth of Wheat and Jawar. This indicates their potential as weedicides. In future the extracts of Araucaria and Thuja can serve as weedicides. Keywords : Allelopathic , Araucaria , Thuja , Wheat , Jawar POSTER SECTION Page 41 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 ETHNO-MEDICINAL PLANT RESOURCES USED AS EVM FOR WOUND HEALING IN BHOR REGION OF PUNE DISTRICT, MAHARASHTRA. P.B. KAMBLE1, A. V.DEO 2AND D.K. KULKARNI 2 1 Department of Botany, Anantrao Thopte College, Bhor - 412 206, India 2 BAIF Development Research Foundation, Warje, Pune-411 058 Ethno-veterinary medicine (EVM) considers that traditional practices of veterinary medicine are legitimate and seeks to validate them. Many non-Western traditions of veterinary medicine exist, such as acupuncture and herbal medicine in China, Tibetan veterinary medicine and, Ayurveda in India. These traditions have written records that go back thousands of years, Many ethno-botanical plants have been used in Indian societies for curing animal diseases. ANTHRA is an organization of women veterinary scientists (NGO), has been documenting and validating EVM since 1996 in different parts of the states of Andhra Pradesh and Maharashtra in India. ANTHRA chose to study EVM because women farmers performed 50 - 90% of all daily activities related to livestock care but were denied aspects of the local EVM because knowledge was traditionally passed from father to son. Documentation of EVM was carried out in Bhor region and16 animal diseases were recorded for each disease different or same plant species and different plant parts were used for Wound maggots and wound healing purpose. Validations of Ethno-Veterinary-Medicines (EVM) were carried out on 97 animals from Bhor region. Pogostemon benghalensis (Burm.f.) O. Ktze. , Colebrookea oppostifolia J. E. Smith, Gnidia glauca (Fresen.) Gilg. Azadirachta indica A. Juss.,. Tridax procumbens L.and Lavandula bipinnata (Roth.) O. Ktze., Annona squamosa L.., and Momordica charantia L. leaf powders were tested for maggoty wound and wound healing. The present paper deals with wild plants used plants for wound maggots and wound healing. Key words: Bhor region, EVM, wound healing, medicinal plants. POSTER SECTION Page 42 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 NUTRITIONAL STUDIES OF SOME NON CONVENTIONAL LEAFY VEGETABLES OF KOLHAPUR DISTRICT OF MAHARASHTRA Vaishali S. Kamble and Varsha D. Jadhav Department of Botany, Shivaji University, Kolhapur- 416004 vsrathod 2003@yahoo.co.in, *meraman2099@gmail.com Kolhapur the extreme Southern district of Maharashtra is a valuable heritage of wild edible plants. The inhabitants and forest dwellers living in hilly regions of the district are still dependent on wild plants for different purposes. These plants are not under cultivation and grows vigorously which are also used as leafy vegetables are said to be non conventional leafy vegetables. These leafy vegetables are chief source of some important nutrients with very low calorie such as sugars, proteins, starch, minerals etc.On the basis of consumption of these leafy vegetables widely, the present paper deals with the nutritional study of some non conventional leafy vegetables such as, Colocassia esculanta (L)Schott.,Portulaca oleracia L.and Tamarindus indica L. The studies indicate that all these plants show variable amount of different nutritionally important components such as proteins, carbohydrates, moisture percentage, some essential mineral elements etc. On the basis of these result it is observed that Portulaca oleracia L. is rich in all these nutrients (Moisture percentage, Sugar, starch, minerals like Ca, Fe etc.). So that these plants may be introduced as nutritionally important plants. NUTRITIONAL STATUS OF WILD JUJUBE FRUIT KANASE R.M, JADHAV A.R, KADAM D.A Department of Botany Y.C.I.S., SATARA rajanikanase@yahoo.com The present work deals with the Nutritional status of a wild fruits of Ziziphus jujube. These fruits are mostly used for edible purpose by triables. These fruits are not cultivated in orchards still there fruits are having palatable tast and pleasant odour. The nutritional status with respect to protein, amino acids, polyphenols, carbohydrates, chlorophyll and sucrose was determined and the results were analysed for discussion. POSTER SECTION Page 43 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 EFFICACY AND EFFECT OF PASSAGE ON THE DEVELOPMENT OF CARBENDAZIM RESISTANCE AGAINST ALTERNARIA ALTERNATA CAUSING ROOT ROT OF FENUGREEK *N.K. Khandare , S.S. Kamble1 and S.N. Khandare2 Mycology and plant pathology research laboratory, Department of Botany, Shivaji University, Kolhapur - 416 004, M.S, India 2 Vidnyan Mahavidyalaya, Sangola, Dist- Solapur 413307, (M.S) India nilkanth_khandare@yahoo.com, Khandaresupriyanil@yahoo.com Fenugreek (Trigonella foenum graecum. L) is native of India and southern Europe. In India it is consumed as vegetable, fodder and source of medicine. Survey of Pathogens infecting Fenugreek from 12 different localities of Maharashtra and Karnataka was carried out. Alternaria alternata (Fr.) Keissler, causing root rot disease to fenugreek is severe in all pathogens infecting Fenugreek. The MIC of carbendazim among 12 isolates of Alternaria alternata on agar plates (in vitro) and root rot of fenugreek (in vivo) exhibit variation. MIC on plates ranged from 5000 to 2500 µg/ml while 500 to 1000 µg/ml on root rot of fenugreek. Isolate (Aa-2) was sensitive. The resistant isolate (Aa- 8) showed 5000 to 2500 µg/ml MIC in vitro and in vivo respectively. Culturing the sensitive isolate of Alternaria alternata (Aa-2) on carbendazim, continuously for eight successive passages showed significantly increased resistance. Use of carbendazim alternately with Aliette, Roko, Zeb- M-45, Curzate and Ridomil MZ for eight successive passages significantly decreased resistance at 3rd passage. Use of carbendazim in mixture with Aliette, Roko, Zeb- M-45, Curzate and Ridomil MZ reduced resistance on agar plates at 2nd passage only. Inoculation of Alternaria alternata on fenugreek continuously for eight successive passages increased the resistance. Carbendazim alternately with Roko, Zeb- M-45, Curzate and Ridomil MZ reduced resistance at 3rd passage while carbendazim in mixture reduced resistance at 2nd passage while Curzate in mixture completely inhibited the growth of pathogen. POSTER SECTION Page 44 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 Phytochemical Screening of Commelinea bengalensis & Cyanotis cristata Kharade Amit S1, Jadhav Sangita S2., Shinde H. L1 , Jadhav S.N1, Kadam D.A1 and Sagar S. S. 1 1 Department of Botany, Y.C.I.S., Satara 415001 (MS), India. 2. Mahatma Phule A.S.C. College, Panvel, Raigad. vishu1415@gmail.com Humans are directly or indirectly dependent upon the products from the Medicinal or common weed plants. Commelina benghalensis and Cyanotis cristata both herb plants belongs to family commelinaceae Distributed in all ranges of India. From the literature available it was learnt that there were No sub stantial work was carried out yet for its chemical constituents. Hence efforts were made made to investigate the secondary metabolites present in leaves of both species. The air dried Leaf powder of both studied species are extracted successively with universal solvent (Distilled water) and alcohol. Preliminary phytochemical investigation of above extract reveled that presence of Saponins, Tannins, Carbohydrates, Glycosides, Flavonids, Alkaloids, and Terpenoids. The present study provides preliminary details to characterize some pharmacognostical parameters of these of Commelinaceae Members. Key words: Phytochemical screening, Commelina benghalensis, Cyanotis cristata. Phytochemical screening of Cythocline maniliana C.P.Raju &R.R.V.Raju for Secondary metabolites Khilare C.J. and Kulkarni P.D. Department of Botany,Dahiwadi college Dahiwadi,Dist Satara.(M.S.) Shahajiraje Mahavidyalaya,Khatav Dist Satara.(M.S.) Plants are source of large amount of drugs comprising to different groups.The traditional medicine involves the use of different plant extracts or the biactive constituents.The study provides health application at affortable costs.Secondary metabolites of plants are responsible for medicinal activity. Root,stem,leaf and inflorescence of Cythocline maniliana was screened by Soxhlet extraction and Ultrasound mathods.Extracts were prepared in various POSTER SECTION Page 45 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 solvents like Acetone,Methanol & Water. Qualitative phytochemical analysis of Root,Stem & Leaf in Acetone extract showed11 different phytochemicals .Methanolic extract of leaf & infls. showed 9 phytochemicals while 6 phytochemicals were detected in aquous extraction.Acetone extract of Root,Stem & Leaf showed prescence of Phenols, Anthraquinons, Flavones, Tannins, Coumarins, Terpenoids, Saponins, Alkaloids, Xanthoproteins, Quinons & Cardioglycosides. The data generated in the present investigation will be usefull in various Chemical industries,Phytopathology,Medical Microbiology,Food preservation etc. It would be benefited for the sustainability of health,hygiene & economy. Phytoremediation - A Novel Strategy for Environmental Cleanup using Plants Sunil Khunte1 Tejas Khunte2 & Jayshree Magdum3 1. S. M. Joshi College, Hadapsar, Pune-28 (M.S.) 2. Sinhgad College of Engineering, Vadgaon(Bk), Pune-41 (M.S.) 3. Mahatma Phule Mahavidhalaya, Pimprigaon, Pune-17 (M.S.) Soil Pollution by heavy metals and other organic or inorganic pollutants is a major environmental problem as it poses a threat to human health. The conventional methods to cleanup such contaminated sites are not always practical as they are costly, environmentally intrusive and cannot be used for larger contaminated sites. Phytoremediation an emerging technology uses plants to remediate soil and water contaminated with various contaminants. Studies indicate that it is a novel strategy that is cost effective, nonintrusive to the environment and can be applied to remediate large contaminated sites. This paper reviews the concept and application of phytoremediation based on our current knowledge field of science. POSTER SECTION Page 46 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 INFLUENCE OF CERTAIN CHEMICALS ON VASE LIFE OF DELPHINIUM MALABARICUM (HUTH) MUNZ. Firdose R. Kolar, Swaroopa R. Ghatge and Ghanasham B. Dixit Laboratory of Cytogenetics and Plant Breeding, Department of Botany, Shivaji University Kolhapur-416004 (M. S.), India. firdose.kousar@gmail.com The vase life of Delphinium malabaricum cut flowers were studied to determine the physiological factors that affect the rate of senescence . Cut spikes of Delphinium were harvested and placed in conical flasks containing 3%, 6%, and 9% sucrose solutions individually and also in combination with Silver nitrate (0.2mM, 0.4mM and 0.6mM ) and 8- hydroxyquinoline (100, 200 and 300 ppm) and water (no chemical treatment). The flasks were kept in the laboratory at room temperature of 23±2OC. Observation on post-harvest parameters were recorded daily, and were monitored in terms of water uptake, change in fresh weight and post-harvest color change. The results of the experiment revealed that the vase life of Delphinium flowers was 5-7 days, which was prolonged up to 12-14 days by the application of chemical preservatives in the holding solution. The holding solution containing 0.4mM AgNO3 + 6% sucrose was determined as the best treatment for the longevity of Delphinium flowers as this treatment recorded the maximum useful vase life. These results indicate that the post-harvest performance and display life of cut Delphinium flowers can be substantially modulated by the use of chemical preservatives in the holding solution. Key words: Delphinium malabaricum, vase life, preservatives, Sucrose, Silver nitrate (AgNO3), 8-hydroxyquinoline (HQ). POSTER SECTION Page 47 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 ETHNOBOANICAL STUDIES CONCERNED WITH MEDICINAL PLANTS IN TRIBAL AREAS OF AHMEDNAGAR DISTRICT (MAHARASHTRA) KULKARNI A.A, B. M GAYKAR & S. S SURYAVANSHI Postgraduate Department of Botany, Ahmednagar College, Ahmednagar Ahmednagar district is not yet well explored ethnobotanically. The medico-botanical surveys in the recent years have revealed that medicinally important plants are abundantly found in the north region of the district, especially in Akole taluka. Plants reported in this investigation are effective in combinations. Plants like Calotropis (Rui) show duel effect. There are certain plants used as antidote to snake poison by tribal of this area. The drug prepared from Solanum nigrum L. leaves is used to cure joundice, also can be used in local medicine against rickets. Unripe fruits of Carica papaya L. Are used for the termination of early pregnancy. Key words: Ethnobotany, medio- ethnobotany Survey of Gamopetalous Angiosperms from Ahmednagar Sangita Kulkarni and B.K. Auti Radhabai Kale Mahila Mahavidyalaya, Ahmednagar 414001 (MS) Ahmednagar is the historical capital of Mughal Emperor Nizam. Now a days it is a district place and is surrounded by rural and rainfed areas receiving very less annual rainfall (below 500mm). It is rich in deciduous plants. A survey is carried out to enumerate plants belonging to gamopetalous Angiosperms during the monsoon season of Year 2012. In all 125 species belonging to 16 families were enumerated from Bhuikot fort, Chandbibi Mahal and Ahmednagar city. The enumerated plants were arranged according to Bentham and Hooker’s system of classification with reference to their habit, family, Botanical name, common name and flowering period. Keywords: Gamopetalae, Angiosperms, Ahmednagar. POSTER SECTION Page 48 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 STUDY OF EFFECT OF WATERLOGGING ON ROOT ANATOMY OF RAGI AND RICE. *Kulkarni, S.S. and Chavan,P.D. * Department of Botany, Shivaji University,Kolhapur MS (India) swarup_136@rediffmail.com In present study effect of waterlogging (4,8 and 12 days) on root anatomy of ragi and rice was studied. It was noticed that the formation of aerenchyma tissue in the ragi roots was induced due to increasing waterlogging treatment. On the other hand,rice roots grown under normal conditions had well developed aerenchyma tissue and treatment of waterlogging did not cause any noticeable change in root anatomy. Thus development of aerenchyma in response to waterlogging in ragi roots would certainly help to supply O2 to the root metabolism. Activity of acid phosphatase was declined in both the species during waterlogging, indicating that its involvement in aerenchyma formation was no any significance. There was decline in calcium content in ragi roots due to waterlogging whereas in rice root an increase was noticeable. Thus a decreased calcium level in ragi roots may limit the availability of calcium for the formation of new cell wall material. Key words: Aerenchyma, Ragi, Rice, Waterlogging STUDY OF ENZYMATIC STATUS IN WILD JUJUBE FRUIT KUMBHAR P.R., PAWAR P.R., KADAM D.A. Department of Botany, Y.C.I.S.Satara. priyakumbhar12@rediffmail.com Enzymes are simple or compound proteins acting as specific catalysts. The present work deals with enzymatic status in wild Zizipus jujube. These fruits are not cultivated in orchards, still these fruits are having palatable taste and pleasant odour. The fruit pulp used for studies of activity of enzymes such as Peroxidase,Nitrate reductase and ATPase and the result analysis is discussed. POSTER SECTION Page 49 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 Mycorrhizal Association of Withania somanifera (L. ) Dunal N. B. Mane1, D. D. Namdas2 and C. J. Khilare3 1 Yashavantrao Chavan Institute of Science, Satara (M.S.) India 2,3 Dahiwadi College, Dahiwadi (M.S.) India nbmane123@gmail.com, ddnamdas@yahoo.co.in, cjk_principal@rediffmial.com Present study has been undertaken to evaluate association of AM fungi from rhizosphere soil of Withania somnifera (L.) Dunal. Total 11 species from 3 genera are observed in which Glomus species are dominantly present in 8.5 pH soil samples. The works have been carried out at Botanic garden of Dahiwadi College Dahiwadi from District Satara. Plants were raised on soil bed in natural environment. Two months old plants were used to study the AM spore population (143±18), mycorrhizal dependency value and growth response. Results obtained from the investigation highlights the correlation between AM spore population, mycorrhizal dependency value and soil parameters like pH, EC, mineral content. Keywords: AM fungi, association, Withania somnifera, soil parameters. COMPARATIVE PHYTOCHEMICAL VARIATION IN DIFFERENT PARTS OF ASHWAGANDHA. Mane1 N. B., Khilare2 C. J., Namdas2 D. D., Naik3 V. V. and Aparadh1 V. T. 1 2, Yashavantrao Chavan Institute of Science, Satara (M.S.) India Department of Botany, Dahiwadi College Dahiwadi (M.S.) 415 508, India. 3, Shivaji University, Kolhapur nbmane123@gmail.com Withania somnifera plant from Solanaceae or nightshade family is known as Ashwagandha, It has been a wider importance in the ayurveda and indigenous medical systems. The main active constituents of this plant are alkaloids and steroidal lactones. The present study deals with comparison of phytochemical analysis in different parts of Withania somnifera. Fresh materials Withania somnifera root, stem, leaf and flower bud were extracted with distilled water and used for screening phytochemical compounds in it by (18) qualitative POSTER SECTION Page 50 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 methods. All parts of this species of Withania indicates positive response to cellulose, carbohydrate, saponin, phenol, tannin and alkaloids test. Arbuscular Mycorrhizal Fungal (AMF) Dynamics along a field grown Linseed from Gondawale B.K. II P. A. Mane, D. D. Namdas and C. J. Khilare Dahiwadi college Dahiwadi An attempt was made to evaluate the quality and quantity of AM Fungi along a field grown Linseed from Gondawale B.K.II, Taluka Man, District Satara. AMF Spore number was found to increase with increase in age of the plants and even more in number in rizospheres.There was a variation in the number of AMF spores in rhizospheric soil ofplants from different locations of field. The plants were found to be infested with various genus/species of AM Fungi. Maximum number of AM Fungal spores were recovered from rizospheric soil of plants 235 per 100 gm Soil and minimum were 95 per 100 gm soil. In general five genera of AM Fungi were found. Among all of these Glomus species were most common in the rhizosphere soil of Linseed in the fields. Keywords: AM Fungi, Dynamics, Rhizospheric soil, Glomus, Gondawale B.K.II . A CHEACK LIST OF ANGIOSPERMIC FLORA OF SHAMGAON GHAT, KARAD, DIST. SATARA . B.D. MOHITE. Department of Botany and Plant protection, Sadguru Gadage Maharaja College, Karad (MS) India. The paper includes a checklist containing a total of 221 angiospermic species (198 Dicot and 23 Monocot) growing in the Shamgaon Ghat, Karad ,Satara,which has been prepared after the survey of the area during the year 2008 to 2010. Key Words: Preliminary checklist , angiospermic flora ,Shamgaon Ghat, Karad, Dist. Satara. POSTER SECTION Page 51 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 ECOLOGICAL STUDIES ON PATESHWAR SACRED GROVE FROM SATARA DISTRICT [MAHARASHTRA], INDIA. S.A. Mohite1 , N.S. Chavan2, S.S. Sathe3 Dept. of Botany,L.B.S. College, Satara. (India). Dept. of Botany,Shivaji University, Kolhapur. (India). Dept. of Botany,P.D.V.P. College, Tasgaon. (India). The paper reports Ecological Studies on Pateshwar Sacred grove from Satara District of Maharashtra state. The plant diversity is varied; unique which comprises typical deccan plateau. The present paper also reports species composition,soil and water anylsis, freqeuncy, density, abundence of flowering plants of Pateshwar Sacred grove. Biodiversity of Basidiomycetes fungus in Panvel Dr. Mrs. Manjiri R. Mote, Mahatma Phule Arts science commerce college Panvel manjirim457@gmail.com The present investigation on Basidiomycetes fungi confined to Panvel Dist. Raigad. Total 10 species were studied belong to 10 genera and 8 families. Of these members the order Tremellales, DacrymycetalesAgaricalles&Lycoperdales were very delicate ephemerals and only appear in short period of rainy season. While members of Aphyllophorales are somewhat leathery or woody and remain for long time thus provide the collections throughout the year. All these forms are saprophytes. Among the five orders studied orderAphyllophorales is dominant and represented by four species belong to four genera and three families. During visit to different locations the collections were quite fruitful and were mostly lignicolous and folicolous saprophytes. The fungi found growing abundantly and luxuriantly on dead organic matter that is tree trunks, barks& valuable woods. POSTER SECTION Page 52 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 Screening of plant weapon production at the stage of infection in Sorghum vulgare Pers. Nikam K. S., Sabale S. R., Ghatage S. Y., Bhosale P. R., Shinde H. L. and Aparadh V. T. Department of Botany, Y.C.I.S., Satara 415001 (MS), india. vishu1415@gmail.com Plant produces secondary metabolites for protection against various pathogenic attacks however they are not required for the plant to survive in healthy condition. Secondary metabolite facilitates the primary metabolism in plants that allow the plant to live. Plants are always surrounded by an enormous number of potential enemies and various kinds of abiotic environmental stress due to their attack heavy reduction in crop productivity. In current article screening of these metabolites in leaves at infected (grain smut and rust disease) and healthy both condition of Sorghum vulgare were carried out by using standard preliminary pharmaconostic tests viz, alkaloids, saponins, phenols, carbohydrates, tannins, flavonoids, sugars, triterpens, phytosterols. Green Synthesis of Silvernanoparticles using the leaf Extract of Bambuseae and study of its antimicrobial activity Nimbalkar D.N ., Mundada P.S., Kadam D.A. Department of Biotechnology, Yashavantrao Chavan Institute of Science, Satara. (MS) The synthesis of nanoparticles from plant sources has proved to be an effective and alternate method for the novel production of nanoparticles. A number of synthesis techniques have been developed including the chemical reduction of silver ions in aqueous solutions, with or without stabilizing agents, thermal decomposition in inorganic solvent, and chemical and photo reduction. The present study deals with the synthesis of silver nanoparticles from Bambuseae leaf extracts and the silver nitrate solution at variable concentrations (0.5mM, 1.0mM, 5.0mM and 10.0 mM). The plant extract was carried out by boiling the fresh leaves in deionized water. The type macromolecules that are mainly involved in the nanoparticle synthesis were studied. U.V Spectrophotometer studies show a graph that shows gradual POSTER SECTION Page 53 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 increase of nanoparticles concentration with time, temperature, and sample characteristics. The bioreduced silver nanoparticles were characterized by UV-Vis spectrophotometer and SEM analysis. The green synthesized nanoparticles were tested for their antimicrobial activity against gram positive and gram negative microorganisms. Key Words: Silver nitrate, Nanoparticles, Bambuseae, Green Synthesis, antimicrobial activity. Phytochemical analysis of medicinal plant Momordica species. Pandit S.S., Jadhav S.M., Satpute M.D., Surve M.M. and Nikam V.K. K.B.P. Mahavidyalaya Pandharpur (MS)-413304 vkn_vijaya@yahoo.co.in An attempt made to study phytochemical analysis of medicinal plant Momordica cymbalaria and Momordica charantia. Leaf chlorophyll and carotenoid content was higher in M. charantia than M. cymbalaria. In case of stem and fruit chlorophyll and carotenoid content has more in M. cymbalaria while root carotenoid level maximum in M. charantia. Total polyphenols and ascorbic acid was comparatively higher in M. cymbalaria. In preliminary investigation of amino acid by TLC shows maximum amino acid bands. STUDY OF TOXICITY IN SOME CUCURBITACEAE MEMBERS PATIL A. S., SAVANT S.H., AHIWALE N.H., APARADH V.T. DEPARTMENT OF BOTANY, Y.C.I.S ,SATARA. ashwinipatil014@gmail.com Antinutritional factors are a wide variety of chemical compounds which affect the nutritive value of food-stuffs when ingested by man or animal. Generally Humans and animals feeding on these climbers. The present work deals with antinutritional activity of Cucurbitaceae members viz; Cucurbita maxima, Lagenaria vulgaris, Luffa aculatangula. It is evidence on result that Cucurbita maxima has higher tannin and nitrate contents than other species of Cucurbitaceae. which may be harmful for human diet. POSTER SECTION Page 54 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 BIODIVERSITY AND ANTIMICROBIAL ACTIVITY OF AGRICULTURAL WEEDS IN KARAD TAHSIL *Bhimrao J. Patil, Suhas Kamble, Atul Madane, Dhanaji Pawar and Archana Shinde *Department of Botany and Plant Protection, Sadguru Gadage Maharaj College, Karad. Dist- Satara. (M. S.) India. 415124 The weeds are the unwanted plant, growing in agricultural fields associated with many crops. Weeds can compete with main crops for food, water, light and shelter and the result is loss in the yield. The field survey was done in the period of 1st June 2012 to 1st January 2013.The weeds are useful for fodder and for compost on the basis of their food value. Some weeds are reported for their antimicrobial activity. In the present work we find out the weeds and their microbial properties. Fourty species were recorded under 37 genera and 18 families. Out of which 35 species show antifungal, 36 species show antibacterial, 37 species show anti insecticidal and all these 40 weed plants show antimicrobial properties. The dominant weeds found in the field are Alternanthera pungen Kunth in H.B. & K., Cassia uniflora Mill, Cynadon dactylon (L.) Pers. and Euphorbia hirta L. Ipomoea obscura (L) Ker.-Gawl. grow especially in the sugarcane fields. All these weeds show better antimicrobial activities. This study was conducted to determine weed diversity. The present work is beneficial to the farmers that they can use such plants for controlling crop diseases and insect pests. Petiolar Anatomical Studies In Some Rubiaceae *Patil, C. R. and D. A. Patil Department of Botany, Rani Laxmibai Mahavidyalaya, Parola-425111. Post-Graduate Department of Botany, S. S. V. P. Sanstha’s L. K. Dr. P. R. Ghogrey Science College, Dhule-424005 (M.S.) India. crpatil_ayush@rediffmail.com, dapatil_10aug@yahoo.com The present authors investigated petiolar anatomy of 14 species belonging to 09 genera of the family Rubiaceae. The petioles (in T.S.) are planoconvex, horse shoe-shaped, circular, heart-shaped and hexagonal in outline. They are usually winged and channeled adaxially. The extent of development of various tissues and their distribution, besides the cell inclusions, are POSTER SECTION Page 55 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 rossete crystals, raphides and tannin. The vasculature is resolved into a conspicuous central arc, besides few other vascular bundles. The central arc is triangular, lunar or crescent shaped, horse shoe-shaped, heart-shaped and circular. The vascular arcs or bundles are capped abaxially by few sclerenchymatous layers, except few. The shapes of petiole, petiolar wings, patterns of vascular supply and association of sclerenchyma with it, cell contents, etc. are thought systematically important. Key Words: Petiolar, Anatomy, Rubiaceae. DIVERSITY IN GALAXY OF FUNGI OF WORLD HERITAGE SITE – KAS (DISTRICT - SATARA.) Chandrahas Patil and Sunetra Patil* Dattajirao Kadam Arts, Science and Commerce College, Ichalkaranji - 415116. *Pre I. A. S. Training Center, Kolhapur - 416004 patilchandrahas07@gmail.com An exhaustive attempts were made to explore fungi wealth of one of the world heritage sites from Maharashtra i.e. Kas Plateau (Dist. Satara). The fungi colonizing the angiosperms, soil, insects, fallen twigs, humus, leaf litter, dung, decaying woods, lichenized fungi on barks and rocks, wood rotting forms as well as fleshy forms belonging to earth balls, earth stars, stink horns, cup fungi, truffels, toadstools, coral-fungi, earth tongues, cantharelleles and from aquatic life (lake, ditches, ponds). An extensive explorations to this site from 2004 to 2010 resulted galaxy of fungi wealth. This heritage site comprising 977 fungal species belonging to 222 genera from myxomycetes, chytridiomycetes, zygomycetes, oomycetes, hyphomycetes, coelomycetes, ascomycetes and basidiomycetes. Hyphomycetes were represented by highest number of species (384), Basidiomycetes by 200 species, Ascomycetes by 173 species, Coelomycetes by 119 species, Chytridiomycetes by 34 species, Myxomycetes by 32 species, Zygomycetes by 29 species and Oomycetes by 7 species. Among the 222 genera from above groups, genus Cercospora represented by 153 species. However, fungal treasures were remained unexplored from biota like aquatic alga, bryophytes, pteridophytes, hyperparasites. POSTER SECTION Page 56 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 Qualitative analysis of secondary metabolites from some filicales members Patil Dipali T., Kadam A. S, Thite S.V., and Kore B.A. sachinthiteg4@gmail.com Yashavantrao Chavan Institute of Science, Satara (M.S.) India The Western Ghats of India is well known for its biodiversity. The pteridophytes are important but much ignored group from this region. This paper deals with qualitative analysis of secondary metabolites from some common filicales plants, viz. Dryopteris filix-max(L.)Scott, Angiopteris evecta(G.forst)Hoffm, Adiantum lunulatum Burm.f., Adiantum incisum Forsk. The qualitative analysis of these plants were carried out for secondary metabolites with reference to saponin, phenol, tannin, phytosterol, triterpens, alkaloids, terpenoids….. etc. Keywords: Filicales, Secondary metabolites, Phytochemicals. To study the comparative enzymatic status of family Cucubitaceae PATIL P.D., TUPE S.B and SHINDE R.A., DEPARTMENT OF BOTANY, Y.C.I.S., SATARA rohinisingh118@gmail.com The paper deals with comparative enzymatic status of family of climbers- Cucurbitaceae. The members of this family are widely used as vegetables. The leaves also have been reported to have medicinal properties. Medicinal properties of fresh leaf extracts are mainly attributed to the enzymes active in the extract. Hence, the fresh leaves of Luffa acutangula and Lagenaria siceraria were used for enzymatic studies such as catalase, nitrate reductase, ATPase and Lipase. The activity of the enzymes studied was found to be more in Lagenaria siceraria than in Luffa acutangula. POSTER SECTION Page 57 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 Comparative analysis of two cultivated varieties of Sorghum under different stress conditions Patil R. B., Chavan P. B., Varnarayan J. S., Gurav S. B., Shinde R. A. and Kadam D. A. Department of Botany, Yashavantrao Chavan Institute of Science, Satara Sorghum is the staple food of MAHARASHTRA. Of the cultivated varieties of Sorghum, Dagadi and Hybrid are most popular among farmers. A comparative analysis of germination studies in the two varieties was done. Dagadi fared better than hybrid variety with respect to shoot length and fresh weight. 7 day old seedlings of the two varieties were subjected to various abiotic stresses like heat, cold, drought and salinity for 2hrs and 24 hrs each. In order to understand the response of the plants to various stresses, a comparative analysis of nitrate reductase and proline accumulation was done. In the first two hours of treatment a remarkable increase in nitrate reductase was observed but after 24 hrs the activity showed progressive decline. Proline content also showed an increase under various stress conditions. Of the two varieties Dagadi responded to drought and cold conditions and Hybrid to drought conditions. FLORISTIC COMPOSITION OF RAJARAM COLLEGE CAMPUS KOLHAPUR PART – I Sunetra C. Patil PRE-IAS TRAINING CENTRE, KOLHAPUR 416004 An attempt has been made to enumerate floristic composition of Rajaram College Campus, Kolhapur. In Part-I, flowering plants from trees and shrubs categories have been enumerated. The campus has occupied 70 acres of land. In present investigations the trees and shrubs which already exist, newly cultivated, introduced (from other countries) flowering plants were listed. The data revealed that, in all 329 flowering angiosperms species belonging to 76 families from 223 genera exist in the campus. There were 165 trees and 164 shrubs from Dicotyledons and Monocolyledons. Out of 329 species, 85 flowering plant species were cultivated in the campus which was introduced (belonging to other countries and found well acclimatized in the campus) while remaining were belonging to Western Ghats of South Western Maharashtra. POSTER SECTION Page 58 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 EFECTS OF LONG CHAIN FATTY ALCOHOL ON SUGARCANE VAR. Co.740 AND Co. 8014. S. S. Patil Doodhsakhar Mahavidyalaya, Bidri. In recent years, much efforts has been expanded to discover and ideal chemical antitranspirant for wide on crops in dry regions of the world. Antitranspirants are applied to the plant foliage to curtail water loss. Since antitranspirants increase plant water potential, that results from antitranspirant treatment need to be measured accurately, R.W.C. is perhaps the most widely accepted method of expressing the quantity of water in plant tissue. R.W.C. under stress could also be used as a measure of tolerance to stress. Proline is stress product. Accumulation of proline in leaves has been shown to be an adaptive mechanism of stress tolerance (Singh et.al.1972). The stomatal diffusive resistance is increased by reducing the transpiration rate and by increasing leaf temperature. Several of the antitranspirants except ABA capable of inducing stomatal closure have proved unsuitable as an ideal antitranspirants, mainly for toxicological reasons and because of undesirable side effects. Hence suppression of stomatal opening by long chain fatty alcohol (LCFA), prompted us to test for its antitranspirant action on intact sugarcane plants under both controlled and water stressed conditions. LIMNOLOGICAL STUDIES OF SOME WATER BODIES IN SATARA CITY. Mrs S.V Patil*. Pawar S.D. Dr.Mrs.Karande V.C., Karande C.T. P.G. Department of Boany Y.C.I.S. Satara Miraj mahavidyalaya,Miraj Present investigation deals with the studies of phytoplankton. Planktonic algae play an important role as primary producers. They form the lowest trophic level in food chain of aquatic ecosystem.They also serve as bioindicators of water quality. Four water bodies viz..Phutake tale, Mahadare reservoir ,Swimming tank and garden pond of Yashvantrao Chavan Institute of science, Satara are selected for present study.Thease are man-made temporary water bodies. POSTER SECTION Page 59 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 Water samples were collected from various sampling stations. Phytoplanktons were identified by using standered litreture.(Desikachary,Prescot etc) Physicochemical parameters were also analysed by using standerd methods( APHA2007) to know the physicochemical environment of these bodies. Altogether 38 species of phytoplankton belonging to four classes were identified, out of 16 species belong to Chlorophyceae ,13 belong to Bacillariophyceae and 5 belongs to Cyanophyceae and 4 species belongs to Euglenophyceae.Among these algae Chlorophyceae members found to be dominant. Key words –Physico-chemical parameters, Phytoplankton ,reservoir, waterbodies Bhudargad fort: A hidden treasure of floristic diversity from Northern Western Ghats N. V. Pawar1, M. S. Nimbalkar2, P. R. Kshirsagar2, Suraj Umdale2 and V. B. Shimpale1 1 Department of Botany, The New College, Kolhapur 2 Sahyadri – GENES, Kawala Naka, Kolhapur Northern Western Ghats are the mountain ranges of Maharashtra, commonly called Sahyadri. Sahyadri has flat top plateaus and forts comprising hidden treasure of flowering plant species. Tourism and overexploitation of natural resources for meeting the local demands for fuel, fodder and food degrades forests of these areas. Accurate and timely information on the floristic composition is of prime importance in judging status of plant resources. In the present work, attempts have been made to assess the plant species composition of Bhudargad fort in different seasons. The work revealed with 319 species of flowering plants, in which some endemic, endangered, critically endangered and medicinal plant species were recorded. Such investigation would be of great help to forest officers, policymakers and community in management of inhabitant plant species of Bhudargad fort. POSTER SECTION Page 60 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 STUDY OF ENZYMATIC STATUS IN ANNONA SQUAMOSA AND PUNICA GRANATUM Pawar P. R., Kumbhar P.R., Kadam D. A. DEPARTMENT OF BOTANY Y.C.I.S.SATARA pawar.pallavi13@gmail.com Enzymes are simple or compound proteins acting as specific catalyst. The present work deals with enzymatic status in Annona squamosa and Punica granatum. The activity of enzymes like Peroxidase, Nitrate reductase, ATPase in the fruits is studied and the result analysis is discussed MEASURING HALOPHYTE DVERSITY ALONG SEA COAST OF GUJARAT A CASE STUDY *Pawar Uday, A. J. Joshi, Department of Life Sciences, Maharaja Krishnakumarsnghji Bhavnagar University, Bhavnagar -364022. Gujarat angiotaxo344@gmail.com Studies on biodiversity have received a special attention after the United Nations Conference of Environment and Development (UNCED) held in 1992 at Rio de Janeiro. According to CBD (Conservation on Biological Diversity), the term ‘Biodiversity’ refers to the variability among living organisms, inter alia, terrestrial marine and other aquatic systems and the ecological complexes of which they are part; this includes diversity within species, between species and of ecosystems. Biodiversity includes three main concepts: genetic diversity, species diversity and ecosystem diversity. Richness and evenness are two main factors in measuring the diversity of a habitat. Richness takes into account individual species, while evenness contributes towards the relative abundance of each species. The diversity of halophytes along Indian coast has virtually remained uninvestigated. According to the results of this study, low to moderate diversity of halophyte flora occurring along ~ 800-km-long sea coast of Gujarat are important from the aspect of biodiversity and species conservation. POSTER SECTION Page 61 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 ANGIOSPERM DIVERSITY OF NANDAGIRI HILL FROM SATARA DISTRICT [MAHARASHTRA], R.R. Sabale , S.A. Mohite, P.S. Jadhav Dept. of Botany, L.B.S. College, Satara. (India). The paper reports angiospermic flora of Nandgiri hill from Satara District of Maharashtra state. The plant diversity is varied; unique which comprises typical deccan plateau. The present paper shows that there are presences of total 57 families, 98 genera with 147 species. Comparative study of photosynthetic pigments and phenolic content in different species of Barleria L. from Satara City N. B. Salunkhe*, A. P. Kadam, J. J. Chavan Department of Botany, Yashavantrao Chavan Institute of Science, Satara – 415 001. nilimasalunkhe90@gmail.com Genus Barleria L. (family - Acanthaceae) comprises over 300 species distributed worldwide. Most of them are wild ornamentals and also having medicinal properties. Extracts of some species are effective against vata, pitta, gingivitis stomatitis, burns, dental cares, inflammation, ascites, edema, wounds, nocturnal ejaculation and crocking heel. Attempts have been made to highlight the quantity of photosynthetic pigments and phenolic contents in three Barleria species. The findings of present study indicated that contents of chl-a, chl-b, total chlorophyll, carotenoids and total phenolics were recorded highest in Barleria grandiflora compared to B. prionitis and B. terminalis. Keywords: Barleria, Chlorophyll, Carotenoids, Total phenolics. POSTER SECTION Page 62 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 Six new and interesting moss records to the Iranian bryoflora Samaneh Akhoondi Darzikolaei1,* and Saeed Shirzadian2 1 Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran 2 Department of Botany, Iranian Research Institute of Plant Protection, P.O. Box 19395-1454, Tehran 1985813111, Iran s.akhoondi90@yahoo.com Studies on Iranian bryoflora were mostly made by foreign botanists, and unfortunately only a few Iranian bryologists have so far paid attention to these interesting plants. In the recent two decades, indigenous botanists have shown more interests on the bryoflora of their own country. In the present study, therefore, six moss species, namely, Bryum algovicum Sendtn. ex Müll. Hal. and B. mildeanum Jur. (Bryaceae), Dicranella subulata (Hedw.) Schimp. (Dicranellaceae), Didymodon sinuosus (Mitt.) Delonge, Syntrichia sinensis (Müll. Hal.) Ochyra, and S. echinata (Schiffn.) Herrnstadt & Ben-Sasson (Pottiaceae) are reported new to the moss flora of Iran. The moss specimens were collected in 2012 from W. Azerbaijan province situated in the western part of Iran. Different characters of above-mentioned mosses were observed under microscope and identified with the help of reliable monographs. Voucher specimens were preserved in the herbarium of the Ministry of Jihad-e-Agriculture ("IRAN") located at the Iranian Research Institute of Plant Protection (Tehran, Iran). ANTINUTRITIONAL ACTIVITY OF SOME GYMNOSPERM SPECIES SAVANT S.H., AHIWALE N.H., GHULE Y. A. AND GAIKWAD S.S. DEPARTMENT OF BOTANY Y.C.I.S ,SATARA. savantsarita@ymail.com. Antinutritional factors are a wide variety of chemical compounds which affect the nutritive value of food-stuffs when ingested by man or animal. Gymnospermic plants shsows hetermorphic alternation of generation type of life cycle. The sporophytic generation is highly evolved in Gymnosperm. Generally in field animals avoid feeding on these plants. The present work deals with antinutritional activity of Gymnospermic plants viz. Cycas, Thuja, Araucaria. It POSTER SECTION Page 63 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 is evidence on result that Thuja occidentalis has higher tannin and nitrate contents than other species of Gymnosperm. Studies on the Effects of Air ions on Plant Growth V. S. Sawant Dept of Physics, Y.C. Institute of Science, Satara, Maharashtra, India. 415001 vssycis@gmail.com In this experiment one seeded tray was exposed to negative air ions, one to positive air ions, and one was kept in the control chamber. Stem length and fresh weight were determined for sets of ten seedlings, selected at random from each tray at interval of 2 days. No drastic ioninduced effects on germination were noted. On the 4th day after seeding, germination had occurred in 85% of the seeds exposed to negative air ions, in 83% of those exposed to positive air ions and in 70% of the controls. The growth of the plants exposed to negative ions was significantly greater than that of the plants exposed to positive air ions. The experiment of growth increase was related to the atmospheric ion density and this in turn determines the magnitude of current flow to ground. Effect of water holding capacity and soil pH on seed germination parameters of ‘Wheat’ (Triticum aestivum L.) and Chickpea (Cicer arietinum L.) N. V. Shedage*, S. S. Shaikh, H. L. Shinde, P. R. Patil, J. J. Chavan Department of Botany, Yashavantrao Chavan Institute of Science, Satara – 415 001 nilamshedage@ymail.com Studies were conducted in the laboratory conditions to determine the effect of water holding capacity (WHC) and pH of the soil on different growth parameters of two important agricultural crops viz. wheat (Triticum aestivum L.) and chickpea (Cicer arietinum L.). Soil samples collected from the 5 different localities of Satara District (Maharashtra) such as Karad, Pusegaon, Satara, Mahabaleshwar and Lonand were analyzed for its WHC, pH and their role in seed germination of wheat and chickpea. The effect of WHC and pH levels on germination frequency and various growth parameters were found to be highly significant. The highest POSTER SECTION Page 64 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 frequency of seed germination of wheat (96%) was recorded in soil collected from Satara (WHC: 44.4%, pH: 8.0) and Karad (WHC: 47.3%, pH: 8.2), while soil collected from Mahabaleshwar (WHC: 52.8%, pH; 7.7) and Lonand (WHC: 30.6, pH: 7.9) was found less significant. However, soil samples having different WHC and pH did not affect the seed germination of chickpea. All the selected soil samples showed significantly higher seed germination in chickpea compared to wheat. The results of the current study indicated that the water holding capacity and pH of the soil significantly affects the seed germination of wheat, however all the selected soil samples favored the highest seed germination in chickpea. Keywords: Seed germination, Water holding capacity, Wheat, Chickpea. Seed coat morphology of Indian species of Barleria L. (Acanthaceae) S. M. Shendage and S. R. Yadav1 Department of Botany, Abasaheb Marathe Arts and New Commerce, Science College, Rajapur. Department of Botany, Shivaji University, Kolhpur. shankar_ms24@rediffmail.com Barleria L. is a pantropical genus of herbs and shrubs comprising some 300 species occurring mainly in Africa and Asia. India comprises about 28 taxa including 26 species, one subspecies and one variety distributed in tropical region. There is a high degree of regional endemism both at the species and sectional level within the genus. Of the total species found in India 24 (about 75%) are endemic to the country (Balkwill and Balkwill, 1997). The seed character is also one of the important criteria useful for taxonomic delimitation of the genus. Seed morphology of 24 species of Barleria was examined by light microscopy. Two groups were distinguished based on morphological variations of quantitative and qualitative features were evaluated to identify group of species using a phonetic analysis. Key Words: Barleria, seed coat, India. POSTER SECTION Page 65 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 RESPONSE OF AM FUNGI ON CHLOROPHYLL AND NUCLEIC ACID CONTENT IN FOUR SUGARCANE CULTIVARS *B.P. Shinde, P.R. Kadlag, K.S. Bhosale P.G. Department of Botany Fergusson College Pune 411 004 India Department of Botany Dr. Babasaheb Ambedkar College Mahad 402 301 India P.G.Department of Botany Nowrosjee Wadia College Pune 411 001 India The effect of inoculation of AM fungi on sugarcane (Saccharum officinarum L.) cultivars was studied by selecting four varieties namely CoVSI- 9805, CoC- 671, Co- 86032 and VSI- 434. These selected four varieties were grown in pots of 18 inch diameter with autoclaved soil. The capacity of the pot was 32 kg soil / pot. Three replicates of both control and experimental plants were maintained. At the time of cultivation 100 g of inoculum containing propagules of Glomus fasciculatum and Scutellospora minuta was added in all experimental pots. The control and experimental pots were placed in field condition for one year. The sugarcane plants were assessed during different growth stages i.e. after 90, 150, 210 and 270 days respectively for chlorophyll and nucleic acid contents. The chlorophyll content of mycorrhizal plants was more than non mycorrhizal plants. On the other hand nucleic acids i. e. DNA and RNA contents were more in control plants than the mycorrhizal plants. The chlorophyll and nucleic acid content were increased during first three stages and it was decreased at last stage of growth. Keywords: A M fungi, Sugarcane, Chlorophyll, Nucleic acid. POSTER SECTION Page 66 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 Alleleopathic effect of basiodiomycetes on germination and growth of Triticum aestivum var Lokvan Shinde M. V., Kadam A. P. and Shinde R. A Yashvantrao Chavan Institute of Science, Satara The paper deals with allelopathic effect of basiodiomycetes on the germination and growth of Triticum aestivum var Lokvan. Aqueous extracts of the fungi Agaricus bisporus and Ganoderma sp were used for the experiments. Increasing concentrations of the extracts (5100%) were used for irrigating the wheat seeds kept under lab conditions. The fungal extracts had a negative effect on the germination and growth of wheat seedlings. The extracts were screened for catalase, ATPase and NR activity to analyze the probable role of these enzymes on the allelopathic effects observed. AEROBIOLOGICAL DIVERSITY OVER GROUNDNUT CROP FIELD AT PATAN, INDIA. Manjusha Shinde*, Minakshi Mahajan**, * Department of Botany, Balasaheb Desai College, Patan (M.S.) India 415206 ** Department of Botany, Fergusson College, Pune, (M.S.) The present paper deals with aerobiological investigation carried out with continuous Tilak air sampler aiming to determine the concentration of aerospora over groundnut crop field in relation to meteorological parameter as well as diseases incidence. Also to determine the diurnal periodicity of some dominant spore types encountered during investigation. The air sampling was carried out during kharif season (rainy season) from 15th July2011 to 14th November 2011 at Patan. During the season PDA culture plates also exposed after one month regularly. The Patan is earthquake prone area having heavy rainfall and is located in the vicinity of Koyana Dam in Western Ghats of Maharashtra. This study revealed incidence of 56 spore types belonging to different classes of fungi and other bio components. Occurrence of some of the dominant spores types in decreasing order are Leptosphaeria(14.66%), Cercospora (10.34%), Aspergillus (7.18%), Pringsheimia (6.57%), Nigrospora (5.43%), Rust Spore(4.68%),Cladosporium(3.26%) while lowest percent POSTER SECTION Page 67 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 concentration was encountered is Diplocladiella (0.011%),Haplosporella (0.063%),Sporormia (0.074%), Hypoxylon (0.14%) and Spermospora (0.74%). Out of this Diplocladiella (0.11%) is very rare in occurrence of aerospora while Spermospora (0.74%) and Ceratosporium (0.1%) are new to aerobiology. The maximum aerospora have been recorded during October 2011. Daily qualitative and quantitative estimation of aerospora revealed maximum incidence on 13 TH October 2011. (13th Oct. D 4039+ N 4567=8606) Incidence of higher concentration of Cercospora (13th Oct 2011 N) is 768, when temp was 250C rainfall 3000mm and R/H was 58% while on 12TH Oct 2011 N. Incidence of Rust spore 625 was highest when R/H 55% temp 250C and which coincide with incidence of Rust and Tikka diseases of groundnut. This finding clearly indicates that wet aerospora is dominant in this area. A probable bacterial fungicide for Powdery mildew diseases Thite S.V., Mundada P.S., Kore B.A. Yashavantrao Chavan Institute of Science, Satara (M.S.) 415002. sachinthiteg4@gmail.com Powdery mildew fungi represent one of the most widely distributed and destructive groups of plant pathogens worldwide. To minimize these losses agrarians have dramatically used pesticides and fungicides which are certainly not eco-friendly. In this context, microbial control through the use of natural antagonistic microorganisms has emerged as a promising alternative. Indeed, these microbial pesticides present many advantages in term of sustainability, mode of action and toxicity compared to chemical pesticides. Here, we focus in details on the versatile utilization of Bacillus based products as microbial pesticide. For current article a culture of Bacillus subtilis, a Gram positive soil inhabiting bacterium has been used. It is an opportunistic pathogen to humans. The in vitro pure culture of B. subtilis in nutrient medium with final density of 1×105 count was used for spray. The effect of this spray was estimated by visual observation for the next 8 days. It was found that the spray of microbial pesticide controls the disease effectively, but needs frequent application. POSTER SECTION Page 68 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 Aerospora survey of Peshwe Park, Pune. Thoke, R. B. Department of Botany, Y.C.I.S., Satara 415001 (MS), India. Aerobiological survey of Peshwe Park was carried out by petriplate exposure method. The study was carried out for a year. A total of 29 species belonging to 16 genera of Zygomycotina and Deuteromycotina were observed on culture plate exposed to cages of animals. Different types of fungi were observed on herbivore and carnivore animals. The common genera are ; Aspergillus, Mucor, Rhizopus, Alternaria, Cladosporium, Curvularia, Drechslera, Humicola, Fusarium, Penicillium & Sclerotium. The fungal spores remain suspended in air and forms the source of aereoallergence. Frequent clearing of cages is suggested to keep free of aereoallergence. Comparative Study of Water Quality Using Physico-Chemical Parameters of Kas Lake and Phutaka Talav From Satara, Maharashtra. P. L. Thorat, Vaishali Shinde, B. A. Kore and A.B. Pawar. Yashavantrao Chavan Institute of Science, Satara. shindewish@gmail.com Good quality of water resources depends on a large number of physico-chemical parameters and biological characteristics. This study is aimed to estimate current status of physico-chemical characteristics and to assess water quality of Kas Lake and Phutaka Talav of Satara. Physico- chemical parameters such as water temperature, pH, turbidity, free CO2, total hardness, chlorides, salinity, dissolved oxygen and biological oxygen demand were analyzed for a period of two months from December 2012 to January 2013. From the present investigation water quantity of Kas Lake is better as compared to the Phutaka Talav. Total hardness of water of water is an indicative of potability of water. Present study states that water from Kas Lake is hygienic while that of Phutaka Talav is unhygienic. POSTER SECTION Page 69 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 Phytochemical Screening in some Cucurbitaceae members Tupe Sachin B., Patil P. D., Thoke R.B and Aparadh V.T. Department of Botany, Y.C.I.S., Satara 415001 (MS), india. vishu1415@gmail.com A Phytochemical is a natural bioactive compound produced by plant body as secondary metabolites (mostly in fruits, vegetables and nuts) that works with nutrients and dietary fiber to protect against pathogenic attack. Phytochemicals are usually associated to plant pigments so, fruits and vegetables that are bright colors- yellow, orange, red, green, blue & purple. Current article deals with the phytochemical screening of Cucurbitaceae members viz- Lagenaria vulgaris, Luffa acutangula & Momordica subangulata. The phytochemical tests of these plants were carried out by standard methods with reference to Alkaloids, Flavonoids, Saponins, Tannins, Carbohydrates, Glycosides, Anthocyanins etc. EFFECT OF AGERATINA AND CATUNAREGAM LEAF LEACHATES ON SEED GERMINATION AND SEEDLING PHYSIOLOGY IN VIGNA RADIATA L *Vaidya, R.R and K.N. Dhumal *Department of Batany, Kisan Veer Mahavidyalaya, Wai. Department of Botany, University of Pune, Pune- 411 007 ramesh.vaidya@rediffmail.com Forest allelopathy plays a major role in understanding the phytosociological nature and species composition of forest ecosystem. Hence the allelopathic impact of some dominant forest plant species from Western Ghat (Mahabaleshwar area) such as Ageratina adenophora Spreng and Catunaregam spinosa (Thunb) Tirveng was investigated. Stimulatory influence of leaf lechates of both the plant forest plants on seed germination, seedling growth and physiology of greengram was reported. The seed germination percentage, root and shoot length, root-shoot ratio as well as fresh and dry biomass have shown significant increase over POSTER SECTION Page 70 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 control. Positive influence of lower concentrations of leaf lechates of both the plants on the content of protein, reducing sugars, total carbohydrates, starch and phenols was noted. However, all the higher concentration of leaf lechates were highly inhibitory for all the above parameters investigated. Leaf lechates of Ageratina adenophora were comparatively more stimulatory at lower concentration and highly inhibitory at higher concentration than Catunaregam spinosa indicating its wide range of allelopathic potential. The stimulatory or inhibitory effect of leaf lechates was ascribed to the allelochemicals present in them. eg. Randianin, oleonelic acid, randialic acid A and B, Iridoid, triterpenoid saponins, beta sitosterol and ageratochromin and methoxychromene, caryphylllin, sequeterpine, beta carophyllin and some monoterpine hydrocarbons might have influenced seedling metabolism and growth in mungbean respectively. Synthesis of Urea Coated Hydroxyapatite Nanoparticles and their potential use as a Nanofertilizers Yadav N.T., Mundada P.S., Kadam D.A. Department of Biotechnology, Yashavantrao Chavan Institute of Science, Satara. (MS) The use of slow release fertilizer has become a new trend to save fertilizer consumption and to minimize environmental pollution. Due to its polymeric cationic, biodegradable, bioabsorbable, and bactericidal characteristics, hydroxyapatite(HA) nanoparticle is an interesting material for use in controlled release systems. However, there are no attempts to explore the potential of hydroxyapatite nanoparticles as controlled release for NPK fertilizers. Nanoparticle suspensions containing nitrogen (N), were evaluated by FTIR spectroscopy. The FTIR results indicated the existence of electrostatic interactions between hydroxyapatite nanoparticles and the N. The stability of the HA colloidal suspension was with the addition of nitrogen, due to the higher anion charge from the calcium phosphate than the anion charges from the urea. Keywords: Hydroxyapatite nanoparticles, Urea, FTIR POSTER SECTION Page 71 NATIONAL CONFERENCE ON RECENT TRENDS IN BOTANY Yashavantrao Chavan Institute of Science, Satara 18th & 19th January, 2013 EFFECT OF SULPHER ON GERMINATION OF GROUNDNUT AND GRAM SEEDS Sanyogita Yadav, Pawar S. G., R. B. Thoke and N. A. Ghanawat Department of Botany, Yashavantrao Chavan Institute of Science, Satara. Groundnut and Gram crop is attacked by many seeds borne fungi like Mucor, Aspergillus, Rhizopus, Fusarium, Penicillium, Rhizoctonia and others. These causes biodeterioration of groundnut and gram seed. The control of these seed borne fungi is essential. In the present study sulpher seed treatment was studied and the incidence of seedborne fungi was recorded. It has been concluded that 1%,2% and 5% sulpher treatment for six hours was found most suitable to control growth of seed borne fungi, without affecting germination of groundnut and gram Seeds. POSTER SECTION Page 72 AUTHOR INDEX MANE N. B., 50 PATIL S. 56 SAWANT S. D. 19 WADKAR P. N. 19 MANE P. A., 12, 51 PATIL S. A., 15 SAWANT V. S. 64 WADVKAR D. S. 28 MERAJ-UL-HAQUE 12 PATIL S. C. 58 SAXENA P. 1, 20 WALAVADE M. N., 37 MOHITE B.D. 51 PATIL S. D. 14 SHAIKH S. S. 65 YADAV N.T., 72 MOHITE P.C. 31 PATIL S. S. 59 SHEDAGE N. V. 65 YADAV S. 72 MOHITE S.A. 52, 62 PATIL S. V. 15, 60 SHENDAGE S. M. 66 YADAV S. R. 66 MOTE M. R. 52 PATIL, A.M. 16 SHENDAGE V.S. 3, 28 MUNDADA P.S. 40, 53, 69, 72 PAWAR A.B. 21, 70 SHIMPALE V. B. 60 PAWAR D. 55 SHINDE A. 55 PAWAR N. V., 60 SHINDE B.P., 2, 5, 66 PAWAR P.R. 49, 61 SHINDE H. L. 40, 45, 53, 65 NAIK V. V. 50 NALWADE A. R 25 NAMDAS D. D. 7, 13, 50, 51 PAWAR S. D., 60 NANDKAR P.B. 12 PAWAR S. G., 72 NIKAM K. S., 53 PAWAR U. 61 NIKAM T.D. 16 RAJGURU Y. R. 19 NIKAM V.K. 54 RAMUGADE M.A., 31 SHINDE R. A. 26, 41, 57, 58, 67 NIMBALKAR D.N. 53 ROHAMARE Y. 16 SHINDE V. 21, 40, 70 NIMBALKAR M. S., 60 SABALE R. 17 SHIRKE. D. R. 14 OULKAR D. P. 17, 19 SABALE R.R., 62 SONAR, B. A. 21 PACHANGANE P. M 13 SABALE S. R., 53 SURVE M. M. 54 PANDIT S.S. 54 SAEED S. 63 SURYAVANSHI S. S 48 PATIL A. S., 54, 23 SAGAR S. S. 18, 45 THITE S. V. 26, 32, 57, 69 PATIL B. J., 55 SALOKHE P. A. 15 PATIL C. R. 55 SALUNKHE N. B., 39, 62 PATIL CHANDRAHAS 56 SALUNKHE V. P. 19 PATIL D. T., 57 SALUNKHE V. S. 30 PATIL D.A. 16, 55 SAMANEH A. D. 63 PATIL H. S., 14 SATHE S.S. 52 PATIL P. D. 57, 70 SATPUTE M. D. 54 PATIL P. R. 32, 65 SAVANT S.H., 23, 54, 64 PATIL R. B. 23, 58 SAWANT I. S.19 SHINDE J. 41 SHINDE M. 68 SHINDE M. V., 67 THOKE, R. B. 69, 70, 72 THORAT P. L., 70 TUPE S.B. 57, 70 UMDALE S. 60 UTTURE S. 17 VAIDYA, R. R. 71 VARNARAYAN J. S., 58 WADAVKAR D. S. 3 ii | P a g e Kamlesh Patel Mobile : 9822500245 With the best Compliments VIJAY TRADING CORPORATION Correspondence Address : 2103/46, Rukmininagar, Old Pune Bangalore Road, KOLHAPUR - 416 005 Telefax: (O) 0231 - 2536711, (R) : 0231 - 2652063. Email : microscopevtc@gmail.com Authorised Distributors for : OLYMPUS – India/Japan Microscopes Dealers in – REMI,ELICO,INCO,LAB-HOSP, YORCO, UNILAB, Instruments, Contech Digital Balances All Kinds of Laboratory Instruments, Equipments, Apparatus, Chemicals, Glasswares. Instrumentation Facility accessible for Research in YCIS, Satara