Towards unraveling relationships in Xylariomycetidae (Sordariomycetes)

Erio Camporesi; Chada Norphanphoun

Xylariomycetidae ( Ascomycota ) is a highly diversified group with variable stromatic characters. Our research focused on inconspicuous stromatic xylarialean taxa from China, Italy, Russia, Thailand and the United Kingdom. Detailed morphological descriptions, illustrations and combined ITS-LSU- rpb 2- tub 2- tef 1 phylogenies revealed 38 taxa from our collections belonging to Amphisphaeriales and Xylariales . A new family ( Appendicosporaceae ), five new genera ( Magnostiolata , Melanostictus , Neoamphisphaeria , Nigropunctata and Paravamsapriya ), 27 new species ( Acrocordiella photiniicola , Allocryptovalsa sichuanensis , Amphisphaeria parvispora , Anthostomella lamiacearum , Apiospora guiyangensis , Ap. sichuanensis , Biscogniauxia magna , Eutypa camelliae , Helicogermslita clypeata , Hypocopra zeae , Magnostiolata mucida , Melanostictus longiostiolatus , Me. thailandicus , Nemania longipedicellata , Ne. delonicis , Ne. paraphysata , Ne. thailandensis , Neoamphisphaeria hyalinosp...

The class Sordariomycetes, which is the second largest class in the phylum Ascomycota, comprises highly diversified fungal groups, with relatively high substitution and evolutionary rates. In this preliminary study, divergence estimates of taxa of Xylariomycetidae are calculated using Ophiocordyceps fossil evidence and secondary data. The combination of fossil and secondary calibration can affect the divergence time estimates by pushing the ages towards the roots. The estimated divergence of Hypocreomycetidae, Lulworthiomycetidae and Xylariomycetidae occurred during the Early Mesozoic (201-252 Mya) and Diaporthomycetidae and Sordariomycetidae occurred later during the Late Mesozoic (145-252 Mya). Two characteristic divergence groups have evolved 168 and 147 Mya and probably provide additional evidence for continuation and species richness of the orders Amphisphaeriales and Xylariales. The early diversifications of the families in Xylariomycetidae have occurred during the Early Cretaceous (100-145 Mya).

Fungal Diversity DOI 10.1007/s13225-015-0340-y Towards unraveling relationships in Xylariomycetidae (Sordariomycetes) Indunil C. Senanayake 1,2,3 & Sajeewa S. N. Maharachchikumbura 4 & Kevin D. Hyde 1,2,3 & Jayarama D. Bhat 6,7 & E. B. Gareth Jones 8 & Eric H. C. McKenzie 9 & Dong Qin Dai 1,2,3 & Dinushani A. Daranagama 3,5 & Monika C. Dayarathne 1,2,3 & Ishani D. Goonasekara 1,2,3 & Sirinapa Konta 3 & Wen Jing Li 1,2,3 & Qiu Ju Shang 3 & Marc Stadler 14 & Nalin N. Wijayawardene 3 & Yuan Pin Xiao 3 & Chada Norphanphoun 3 & Qirui Li 3,10 & Xing Zhong Liu 5 & Ali H. Bahkali 8 & Ji Chuan Kang 10 & Yong Wang 10 & Ting Chi Wen 10 & Lucile Wendt 15 & Jian Chu Xu 1,2 & Erio Camporesi 11,12,13 Received: 30 May 2015 / Accepted: 29 June 2015 # School of Science 2015 Abstract The classification of subclass Xylariomycetidae is revisited with additional collections and phylogeny based on novel rDNA sequence data. Phylogenetic inferences are provided and are based on analysis of 115 sequence data, including new data for 27 strains. An updated outline to the subclass is presented based on the phylogenies and comprises two orders, 18 families and 222 genera. An account of each order, family and genus in the subclass is given. We accept the orders Amphisphaeriales and Xylariales based on morphological and phylogenetic evidence. Amphisphaeriales comprises Amphisphaeriaceae, Bartaliniaceae fam. nov., Clypeosphaeriaceae, Discosiaceae f a m . n o v. , P e s t a l o t i o p s i d a c e a e f a m . n o v. a n d Phlogicylindriaceae fam. nov. Xylariales comprises Apiosporaceae, Cainiaceae, Coniocessiaceae, Diatrypaceae, Graphostromataceae (doubtful), Hyponectriaceae, I o d o s p h a e r i a c e a e , L o p a d o s t o m a c e a e f a m . n o v. , Melogrammataceae, Pseudomassariaceae fam. nov., Vialaeaceae and Xylariaceae. The new genera and species introduced are Arthrinium hyphopodii, A. subglobosa, C a i n i a a n t h o x a n t h i s , C i f e r r i a s c o s e a g e n . n o v. , C. fluctamurum, C. rectamurum, Discosia neofraxinea, D. pseudopleurochaeta, Hyalotiella rubi, Seimatosporium cornii, S. ficeae, S. vitis and Truncatella spartii. * Kevin D. Hyde kdhyde3@gmail.com 7 Department of Botany, Goa University, Goa 403 206, India 8 Department of Botany and Microbiology, King Saudi University, Riyadh, Saudi Arabia 1 Key Laboratory for Plant Biodiversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Science, Kunming 650201, Yunnan, China 9 Manaaki Whenua Landcare Research, Private Bag 92170 Auckland, New Zealand 10 2 East and Central Asia, World Agroforestry Centre, Kunming 650201, Yunnan, China Engineering Research Center for Southwest Bio-Pharmaceutical Resources, Ministry of Education, Guizhou University, Guiyang, Guizhou 550025, China 3 Centre of Excellence for Fungal Research, Mae Fah Luang University, Chiang Rai, Thailand 11 A.M.B. Gruppo Micologico Forlivese, Antonio Cicognani, Via Roma 18, Forlì, Italy 4 Guizhou Key Laboratory of Agricultural Biotechnology, Guizhou Academy of Agricultural Sciences, Guiyang 550006, Guizhou, China 12 A.M.B. Circolo Micologico, Giovanni Carini, 314 Brescia, Italy 13 Società per gli Studi Naturalistici della Romagna, 144 Bagnacavallo, RA, Italy 14 Department of Microbial Drugs, Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstrasse 7, 38124 Braunschweig, Germany 15 Department of Microbial Drugs, Braunschweig, Helmholtz Centre for Infection Research (HZI), Braunschweig, Germany 5 6 State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, No 3 1st West Beichen Road, Chaoyang District, Beijing 100101, China No. 128/1-J, Azad Housing Society, Curca, P.O. Goa Velha 403108, India Fungal Diversity Keywords Amphisphaeriales . Apiosporaceae . Bartaliniaceae . Cainiaceae . Ciferriascosea . Clypeosphaeriaceae . Coniocessiaceae . Discosiaceae . Diatrypaceae . Hyponectriaceae . Hypoxyloideae . Iodosphaeriaceae . Lopadostomaceae . Melogrammataceae . Pestalotiopsidaceae . Phlogicylindriaceae . Pseudomassariaceae . Vialaeaceae . Xylariaceae . Xylarioideae Introduction The order Amphisphaeriales was introduced by Eriksson and Hawksworth (1986), and in most subsequent cases was treated as a synonym of Xylariales. The family Amphisphaeriaceae was introduced by Winter (1884–1886) as “Amphisphaerieae”, based on the type genus Amphisphaeria. It was distinguished from other families in having partially immersed ascomata in the substrate and from its unique shape of the ostiolar canal. Amphisphaeriaceae was reintroduced by Müller and von Arx (1962) based on the presence of a small iodine positive ring or disc in the ascal apex and in having ascomata immersed under a clypeus (Kang et al. 1999b). Hawksworth et al. (1995) included Amphisphaeriaceae in the order Xylariales. The Xylariales was initially included in the class Hymenoascomycetes by Barr (1990), but later placed in the subclass Xylariomycetidae (Eriksson and Winka 1997). Several of the most well-known species may form conspicuous stromata and are clearly visible on the surface of decaying plants (Kuhnert et al. 2013). Krug (1978) introduced the family Cainiaceae to accommodate the genus Cainia whose species were previously placed under Amphisphaeriaceae. During a revision of the genera of Calosphaeriaceae, Barr et al. (1993) introduced Graphostromataceae as a monotypic family in the order Xylariales based on the nodulisporium-like conidial morph and diatrype-like sexual morph having allantoid ascospores. The family Apiosporaceae was introduced by Hyde et al. (1998). Later, Crous and Groenewald (2013) used molecular data to confirm the placement of this family in Xylariales, as a sister group to Amphisphaeriaceae. Coniocessiaceae was introduced by Asgari and Zare (2011), placed in Xylariales and comprised of the single genus Coniocessia with four species. Vialaeaceae was introduced by Cannon (1995) for species with isthmoid ascospores. Recent studies have provided phylogenetic evidence that this is a well-supported family in Xylariales (Shoemaker et al. 2013; McTaggart et al. 2013; Senanayake et al. 2014). LSU molecular data have shown a close relationship between Amphisphaeriaceae, Cainiaceae, Clypeosphaeriaceae and Hyponectriaceae (Jeewon 2001) in Xylariales. Also, in an attempt to show the evolutionary relationships among the accepted families of this order, Smith et al. (2003) used molecular data, which resulted in showing Xylariales as a monophyletic group containing Amphisphaeriaceae, Apiosporaceae, Clypeosphaeriaceae, Diatrypaceae, Graphostromataceae, Hyponectriaceae and Xylariaceae. However, the use of 28S and 18S alone proved to be inadequate for determining the relationships between these families. Lumbsch and Huhndorf (2010) treated the order Xylariales with six families and 154 genera. Currently 11 families have been listed for Xylariales, namely; Amphisphaeriaceae, Apiosporaceae, Cainiaceae, Clypeosphaeriaceae, Coniocessiaceae, Diatrypaceae, Graphostromataceae, Hyponectriaceae, Melogrammaceae, Vialaeaceae and Xylariaceae (Maharachchikumbura et al. 2015). Renewed interest in the group Xylariales has resulted in the publication of monographs for the genera Daldinia, Pestalotiopsis and Rosellinia (Petrini 2013; Maharachchikumbura et al. 2014; Stadler et al. 2014). The amphisphaeriaceous members of Xylariales are however, still relatively poorly understood and there is no natural classification developed for the group. As a result of various collections of microfungi from China, Italy and Thailand, we have encountered several genera of Amphisphaeriales and subjected them to morphomolecular studies. The phylogeny resulting from the analyses of a combined LSU and ITS gene dataset show that our understanding of the order is fragmentary. In this paper, we resurrect the order Amphisphaeriales and provide a natural classification for the taxa of Xylariomycetidae. Materials and methods Specimen collection, morphological examination and photomicrography Fresh, plant materials, such as leaves, twigs, stems and bark were collected from different geographical localities in China, Italy and Thailand. Fresh materials were placed in paper bags and all collection details were noted. Specimens were brought to the laboratory and examined under a stereomicroscope to observe the characteristics of stromata and fruiting bodies. Macro-morphological characters were photographed by a digital camera fitted to the stereomicroscope. A few fruiting bodies were transferred to a drop of water mounted on a slide using a fine needle or forceps for rehydration and they were crushed to show internal structures. Hand sections of fruit bodies were made by a razor blade and mounted in a water drop. Morphological characteristics of ascomata/conidiomata, asci, ascospores/conidia and other tissues associated with ascomata were photographed using a digital camera fitted with the compound microscope. Melzer’s reagent, Indian ink and 5 % KOH were used when necessary. All microphotographs were arranged using Adobe Photoshop CS3 extended (v. 10.0) and all measurements were made with Tarosoft image Fungal Diversity framework (v. 0.9.0.7). Specimens were preserved and deposited at accessible herbaria. Single spore isolation Few fruiting bodies were picked from the substrate using a sterilized needle and placed in few drops of sterilized distilled water mounted on a sterilized cavity slide as detailed in Chomnunti et al. (2014). Fruiting bodies were crushed to separate the spores and obtain a spore suspension. The suspension was inoculated on to fresh Malt Extract Agar (MEA) or Potato Dextrose Agar (PDA) plates and incubated at room temperature. Germinating ascospores/conidia were aseptically transferred to fresh culture plates. Colonies were photographed and characters noted. Living cultures are deposited at BCC, MFLU, GU and ICMP culture collections. Promoting asexual morphs Dried and sterilized, grass leaves or pine needles were laid parallel to the colony margin of pure cultures which were grown on water agar. Conidiomata produced on both water agar and sterilized pine needles were observed after few weeks of incubation at 20 °C. Morphological characteristics were studied as mentioned above. Herbarium specimen examination Types and other relevant authentic specimens were loaned from different accessible herbaria (G, MFLU, NY, PDD, S, WU). A small part of the herbarium specimen was cut and rehydrated using 5 % KOH. Micro-morphological characters were observed from rehydrated ascomata and photography was done as previously described. Some specimens were redrawn based on previously illustrated authentic specimens. DNA extraction, PCR amplification, sequencing and phylogenetic analyses Fresh fungal mycelium grown on MEA or PDA for 4 weeks at 20 °C was scraped from the colony margin and were used for genomic DNA extraction using a modified protocol of Doyle and Doyle (1987) and Lee and Taylor (1990). PCR amplification and sequencing of LSU region using the primer pair LROR/LR5 and ITS region using primer pair ITS5/ITS4 was performed (Vilgalys and Hester 1990; White et al. 1990). The PCR products were visualized on 1 % agarose electrophoresis gel stained with ethidium bromide. Purification and sequencing of PCR products were carried out at BGI Ltd. Shenzhen, PR China. The DNA sequences generated from this study were deposited at GenBank. The sequences generated in this study were supplemented with additional sequences obtained from GenBank (Table 1) based on blast searches and published literature. Multiple sequence alignments were generated with MAFFT v. 7 (http:// mafft.cbrc.jp/alignment/server/index.html); the alignment was improved with MEGA v. 5.2.2 (Kumar et al. 2012) or BioEdit v. 7.0.5.2 (Hall 1999). The phylogenetic analyses were performed for Maximum Likelihood in RAxML 7.4.2 Black Box or RAxMl GUI (Stamatakis 2006; Stamatakis et al. 2008), Maximum Parsimony in PAUP v. 4.0b10 (Swofford 2003), or Bayesian inference in MrBayes v. 3.2.1 (Ronquist et al. 2012) as specified in the legend of each phylogenetic tree. Results and discussion The phylogeny resulting from the analysis of LSU and ITS sequence data is shown in Fig. 1. Overall, the topologies obtained from the different phylogenetic analyses were similar. The separation between Xylariales and Amphisphaeriales is not well-supported, however morphologically they are distinct and we treat them as distinct orders. The order Amphisphaeriales separates into nine clades labeled as A-H with varying support. Clade A has weak support and is represented by Discosiaceae fam. nov. Species of Adisciso, Discosia, Discostroma, Sarcostroma and Seimatosporium grouped in Clade A in congruence with previous findings (Maharachchikumbura et al. 2014; Li et al. 2015a). Species of Discostroma and Seimatosporium have been linked by molecular data (Tanaka et al. 2011), however the molecular links have not been proven for the types of either genera. Although the support is weak, we introduce this as a new family because of morphological similarities in the included genera and the fact that this group of genera always cluster together in analyses. Clade B includes Ciliochorella, Lepteutypa, Monochaetia, Neopestalotiopsis, Pestalotiopsis, Pseudopestalotiopsis and Seiridium and are accommodated in a new family, Pestalotiopsidaceae introduced in this paper. Both morphological similarities and sequence analysis support their status as a family. Clade C includes species of Bartalinia, Broomella, Dyrithiopsis, Hyalotiella, Truncatella and Zetiasplozna and a new family Bartaliniaceae is introduced to accommodate these taxa. Both morphological similarities and sequence analysis support their establishment as a family. Clades D include the genera Immersidiscosia and Ellurema, and are phylogenetically closely related to Discosiaceae, Pestalotiopsidaceae and Bartaliniaceae. However, the relationships of Immersidiscosia and Ellurema to any of the above families are poorly resolved and therefore the genera are placed in Amphisphaeriales genera incertae sedis (the LSU region of Ellurema indica isolate MIU43478, accession: U43478, needs resequencing as it has a short sequence, approximately around 450 bp). Clade E comprises of two species of the asexual genus Phylogicylindria and two species of the sexual genus Ciferriascosea and these are Fungal Diversity Table 1 Isolates sequenced in this study and their GenBank and culture accession numbers Taxon name Culture accession no Genbank accession no LSU ITS Amphisphaeria sorbi MFLUCC 13–0721 KP744475 KR092797 Annulohypoxylon multiforme Arthrinium hyphopodii Arthrinium subglobosum Bartalinia robillardoides Biscogniauxia nummularia Broomella vitalbae Broomella vitalbae Cainia anthoxanthis Cainia graminis Ciferriascosea rectamurum Ciferriascosea fluctamurum Diatrype disciformis Daldinia concentrica Discosia neofraxinea Discostroma fuscellum Hyalotiella rubi Eutypa flavovirens Hyalotiella spartii Kretzschmaria deusta CBS119016 MFLUCC 15–0003 MFLUCC 11–0397 MFLUCC 12–0070 MUCL 51395 MFLUCC 14–1000 MFLUCC 13–0798 MFLUCC 15–0539 MFLUCC 15–0540 MFLUCC 15–0542 MFLUCC 15–0541 MFLUCC 15–0538 CBS113277 MFLUCC 13–0204 MFLUCC 14–0052 MFLUCC 13–0660 MFLUCC 13–0625 MFLUCC 13–0397 CBS 163.93 KT281893 KR069111 KR069113 KR559738 KT281894 KP757750 KP757749 KR092777 KR092781 KR092776 KR092778 KR092784 KT281895 KR072672 KT005514 KR092775 KR092774 KP757752 KT281896 KC477234 KR069110 KR069112 KT162919 JX658444 KP757754 KR092788 KR092787 KR092793 KR092786 KR092789 KR092795 AY616683 KR072673 KT005515 KR092799 KR092798 KP757756 KT281901 Podosordaria tulasnei Poronia punctata Pseudomassaria chondrospora Pseudomassaria corni Sarcoxylon compunctum Seimatosporium cornii Seimatosporium rhombisporum CBS 128.80 CBS 656.78 MFLUCC 15–0545 MFLUCC 15–0544 CBS 359.61 MFLUCC 14–0467 MFLUCC 15–0543 KT281897 KT281900 KR092779 – KT281898 KR559739 KT281902 KT281904 KR092790 KR092791 KT281903 KT162918 Seimatosporium ficeae Seimatosporium rosae Seimatosporium vitis Truncatella spartii Vialaea mangiferae Xylaria obovata Xylaria polymorpha MFLUCC 15–0519 MFLUCC 14–0621 MFLUCC 14–0051 MFLUCC 15–0537 MFLUCC 12–0808 MFLUCC 13–0115 MUCL 49884 KR092780 KR920686 KT198727 KR920362 KR092782 KF724975 KR049089 KT281899 KR092792 KR092800 KT198726 KR920363 KR092794 KF724974 KR049088 FN689809 accommodated in a new family, Phylogicylindriaceae introduced in this paper (note: the LSU sequences of Ciferriascosea are rather short; accession: KR092778, KR092776). Clade F is represented by the family Clypeosphaeriaceae and is well-separated from Amphisphaeriaceae sensu stricto. Clade G has good support and represents Amphisphaeriaceae sensu stricto. Two Amphisphaeria species are included here. Clade H is represented by P. carolinensis (IFO 9502), a taxon that may not belong to Pseudomassaria. The same observation was reported by Jaklitsch and Voglmayr (2012). The order Xylariales comprises 14 clades with varying support labeled I-V. Clade I represents the xylaroid clade of Xylariaceae which includes the epitype of Xylaria hypoxylon Fig. 1 Consensus tree resulting from a Maximum Likehood analysis of the combined LSU and ITS sequence alignment of Xylariomycetidae and other isolates in Sordariomycetes. Families are indicated in coloured blocks. RAxML bootstrap support values (MLB above 50 %), Bayesian posterior probabilities (PP above 90 %) and maximum parsimony bootstrap support values (MPB above 50 %) are given at the nodes (MLB/PP /MPB). The scale bar represents the expected number of changes per site. The tree was rooted to Dothidea sambuci and Botrytis fuckeliana Fungal Diversity 77/ 1.0/ 54 Discostroma tostum NBRC 32626 Seimatosporium rosae MFLUCC 14−0621 Seimatosporium cornii MFLUCC 14–0467 66/ 0.91/ 61 Discostroma fuscellum MFLUCC 14–0052 Seimatosporium hypericinum NBRC 32647 78/ 1.0/ 80 Seimatosporium rhombusii MFLUCC 15–0543 100 / 1.0/ 99 Seimatosporium ficeae MFLUCC 15–0519 90/ 1.0/ 82 Discosiaceae Sarcostroma restionis CBS 118154 77/ 1.0/ 51 Seimatosporium eucalypti CBS 115131 -/ 0.90/ Discosia neofraxinea MFLUCC 13–0204 Adisciso yakushimense MAFF 242774 87/ 1.0/ 92 Adisciso tricellulare NBRC 32705 98/ 1.0/ 98 Discosia pini MAFF 410149 100/ 1.0/ 100 Discosia artocreas NBRC 8975 Lepteutypa cupressi IMI 052255 55/ -/ Ciliochorella castaneae HHUF 28799 52/ 0.92/ Seiridium phylicae CPC 19965 50/ 1.0/ Seiridium cardinale CBS 172.56 Seiridium papillatum CBS 340.97 53 / Monochaetia kansensis PSHI2004Endo1032 100/ 1.0/ 100 Pestalotiopsaceae Monochaetia kansensis PSHI2004Endo1030 74/ 0.95/ Pseudopestalotiopsis cocos CBS 272.29 66/ -/ 70 57 Pseudopestalotiopsis theae MFLUCC 12–0055 100/ 1.0/ 100 Neopestalotiopsis protearum CBS 114178 100/ 1.0/ 100 Neopestalotiopsis rosae CBS 101057 99/ 1.0/ 92 Pestalotiopsis malayana CBS 102220 100/ 1.0/ 100 Pestalotiopsis knightiae CBS 114138 50/ 0.92/ 68 Truncatella spartii MFLUCC 15–0537 82/ -/ 93/ -/ 57 Truncatella spartii MFLUCC 15–0573 67/ -/ - Truncatella sp. HKUCC 7987 88/ -/ Truncatella restionacearum CMW 18755 -/ 1.0/ 84 Truncatella hartigii CBS 118148 Dyrithiopsis lakefuianensis HKUCC 7303 Zetiasplozna acaciae CPC 23421 Bartalinia robillardoides CBS 122705 Bartaliniaceae -/ -/ 94 Bartalinia robillardoides MFLUCC 12–0070 Hyalotiella rubi MFLUCC 13–0660 Hyalotiella spartii MFLUCC 13–0397 Broomella vitalbae MFLUCC 14–1000 99/ 1.0/ 100/ 1.0/ 100 98 Broomella vitalbae MFLUCC 13–0798 100/ 1.0/ 100 Truncatella laurocerasi ICMP 11214 88/ 1.0/ 88 Truncatella angustata ICMP 7062 Ellurema indica MIU 43478 -/ 0.96/ Immersidiscosia eucalypti NBRC 104195 100/ 1.0/ 99 Immersidiscosia eucalypti MAFF 242781 Ciferriascosea wavyseptata MFLUCC 15–0541 80/ 0.98/ Ciferriascosea uniseptata MFLUCC 15–0542 78/ 0.98/ 58 54/ 0.98/ Phlogicylindriaceae Phlogicylindrium eucalyptorum CBS 111689 100/ 1.0/ Phlogicylindrium uniforme CBS 131312 100 Clypeosphaeria uniseptata HKUCC 6349 100/ 1.0/ 100 Clypeosphaeriaceae Clypeosphaeria uniseptata AY083830 98/ 1.0/ 95 Amphisphaeria umbrina HKUCC 994 76/ -/ 87 Amphisphaeriaceae Amphisphaeria sorbi MFLUCC 13–0721 69/ 0.99/ Pseudomassaria carolinensis 9502 (IFO) 87/ 1.0/ 80 a b c d e f g h Fungal Diversity Podosordaria tulasnei CBS 128.80 Xylaria hypoxylon CBS 122620 Xylaria obovata MFLUCC 13–0115 79/ 0.98/ Xylaria polymorpha MUCL 49884 99/ 0.98/ 96 Kretzschmaria deusta CBS 163.93 Poronia punctata CBS 656.78 52/ 0.92/ 76/ 1.0/ 50 Sarcoxylon compunctum CBS 359.61 Biscogniauxia nummularia MUCL 51395 95/ 1.0/ 91 Camillea obularia ATCC 28093 88/ 0.97/ 91 60/ 0.98/ 66/ -/ - “Xylarioideae” Xylariaceae i 67/ 0.96/ - Graphostroma platystoma AFTOL ID 1249 Graphostromataceae Hypoxylon fragiforme MUCL 51264 95/ 1.0/ 77 “Hypoxyloideae” Xylariaceae Daldinia concentrica CBS 113277 99/ 1.0/ 72 Annulohypoxylon multiforme CBS 119016 100/ 1.0/ 64 Cainia graminis CBS 136.62 -/ 0.98/ 100/ 1.0/ 100 Cainia anthoxanthis MFLUCC 15–0539 92/ 1.0/ 62 Cainia graminis MFLUCC 15–0540 Atrotorquata spartii MFLU 14–0738 Cainiaceae 99/ 0.99/ 79 Seynesia erumpens SMH 1291 Amphibambusa bambusicola MFLUCC 11–0617 -/ -/ 70 Arecophila bambusae HKUCC 4794 Lopadostoma americanum HV-2014h LG8 64/ -/ 84/ 0.99/ 84 Lopadostoma dryophilum LG21 89/ 1.0/ 97 Lopadostoma quercicola HV-2014a strain LG27 Lopadostomaceae Lopadostoma turgidum LT2 94/ 1.0/ 78 -/ -/ 79 Lopadostoma fagi HV-2014f LF1 Creosphaeria sassafras CM AT-018 -/ -/ 58 Coniocessia nodulisporioides CBS 281.77 100/ 1.0/ 100 Coniocessiaceae Coniocessia maxima strain Co117 - / 0.98/ Diatrype disciformis MFLUCC 15–0538 -/ 1.0/ 84 Eutypa lata CBS 208.87 Diatrypaceae Diatrype palmicola MFLUCC 11–0018 97/ -/ 58 Eutypa flavovirens MFLUCC 13–0625 Diatrype whitmanensis ATCC MYA-4417 95/ -/ 63 Apiospora bambusae ICMP 6889 84/ 1.0/ 97 Apiospora setosa ICMP 4207 82/ 1.0/ 89 Arthrinium sp. MFLUCC 15–0002 -/ 0.90/ Apiosporaceae 82/ 1.0/ 95 Arthrinium phaeospermum HKUCC 3395 Arthrinium hyphopodii MFLUCC 15–0003 82/ 0.99/ Arthrinium subglobosum MFLUCC 11–0397 100/ 1.0/ Pseudomassaria chondrospora MFLUCC 15–0545 100 Pseudomassaria chondrospora PC1 Pseudomassariaceae 100/ 1.0/ 100 Pseudomassaria sepincoliformis CBS 129022 Hyponectria buxi UME 31430 Hyponectriaceae Melogramma campylosporum MBU Melogrammataceae Squamotubera sp. HKAS79008 100/ 1.0/ 100 Squamotubera sp. HKAS78918 99/ 0.99/ 100 Iodosphaeria phyllophila FJS8 Iodosphaeriaceae 81/ 0.97/ 79 100/ 1.0/ 97 Repetophragma inflatum NN 42958 Sporidesmium knawiae CPC 15467 Vialaea minutella BRIP 56959 100/ 1.0/ 100 Vialaeaceae Vialaea mangifia MFLUCC 12–0808 Sordaria fimicola HKUCC 3714 97/ 1.0/ 95 Sordariomycetidae Apiosordaria verruculosa F-152365 93/ 0.99/ 54 Meliola centellae VIC 31244 100/ 1.0/ 100 69/ 0.98/ Meliolomycetidae Asteridiella obesa VIC 31239 Cryptodiaporthe aesculi AFTOL-ID 1238 100/ 1.0/ 100 Diaporthomycetidae Ophiodiaporthe cyatheae YMJ 1364 84/ 0.99/ Hypocrea rufa DAOM JBT1003 Hypocreomycetidae 84/ 1.0/ Reticulascus clavatus CBS 125296 Dothidea sambuci DAOM 231303 Botryotinia fuckeliana AFTOL-ID 59 j k l m n o p q r s t u v 0.2 Fig. 1 (continued) Fungal Diversity (CBS 122620), the type species of Xylaria. However the strains in this subfamily have short LSU sequence data. Other species groups in the clade I are Podosordaria, Kretzschmaria, Poronia and Sarcoxylon. Clade J is represented the hypoxyloid clade of Xylariaceae. However, within the clade J includes the monotypic family Graphostromataceae represented by the generic type Graphostroma platystoma, showing affinities with Biscogniauxia Kunze and Camillea Fr (see notes under Graphostroma). Clade K is the family Cainiaceae comprising Amphibambusa bambusicola, Arecophila bambusae, Atrotorquata spartii, Cainia spp. and Seynesia erumpens. Atrotorquata was previously placed in Amphispharaceae (Lumbsch and Huhndorf 2007; Maharachchikumbura et al. 2015) and Kang et al. (1999a, b) pointed out that it morphologically resembles Cainia. Clade L represents a newly introduced family Lopadostomaceae. Generally the obtained data indicate a close relationship between Lopadostoma and Creosphaeria and is in agreement with Jaklitsch et al. (2014). All Coniocessia LSU sequence data in GenBank are short and clade M represents the family Coniocessiaceae. Another significant clade was formed by the family Diatrypaceae and is represented by clade N as a sister group to Lopadostomaceae and Coniocessiaceae. Diatrypaceae comprises Eutypa / Diatrype species. Another significant clade is the family Apiosporaceae (Clade O) and Crous and Groenewald (2013) analyzed LSU rDNA sequence data and confirmed that the Apiosporaceae belongs in Xylariales, and is a sister group to Amphisphaeriaceae. Apiosporaceae was a sister group to Pseudomassariaceae in this study. Clade P corresponds to Pseudomassaria and is accommodated in a new family, Pseudomassariaceae. Based on LSU sequence data, Jaklitsch and Voglmayr (2012) reported anomalies in the taxonomic position of Hyponectira buxi and they questioned the reliability of this strain. In the present study it (clade Q) form a sister clade to family Melogrammataceae. Clade R comprises the monotypic family Melogrammataceae. The genus Squamotubera is represented by clade S. Sequences analysis of Iodosphaeria (clade T) results in a distinct taxon and in the family Iodosphaeriaceae. Clade U is represented by Sporidesmium knawiae which is considered as synonymous to the sister species, Repetophragma inflatum. Clade V comprises of two species of Vialaea in the family Vialaeaceae. Taxonomy Outline of Xylariomycetidae $ =Sequence data in GenBank as at date on submission Subclass Xylariomycetidae O.E. Erikss & Winka Order Amphisphaeriales D. Hawksw. & O.E. Erikss. Amphisphaeriaceae G. Winter Amphisphaeria Ces & De Not. $ Bartaliniaceae Wijayawardene et al. Bartalinia Tassi$ Broomella Sacc$ Dyrithiopsis L. Cai et al. $ Hyalotiella Papendorf$ Truncatella Steyaert$ Zetiasplozna Nag Raj $ Clypeosphaeriaceae G. Winter Apioclypea K.D. Hyde$ Aquasphaeria K.D. Hyde Brunneiapiospora K.D. Hyde et al. $ Clypeosphaeria Fuckel$ Crassoascus Checa et al. Palmomyces K.D. Hyde et al. $ Discosiaceae Maharachch. & K.D. Hyde Adisciso Kaz. Tanaka$ Discosia Lib. $ Discostroma Clem. $ Sarcostroma Cooke$ Seimatosporium Corda$ Pestalotiopsidaceae Maharachch. & K.D. Hyde Ciliochorella Syd. $ Neopestalotiopsis Maharachch. et al. $ Pestalotiopsis Steyaert$ Pseudopestalotiopsis Maharachch. et al. $ Seiridium Nees$ Phlogicylindriaceae Senan., & K.D. Hyde Ciferriascosea Senan., et al. $ Phlogicylindrium Crous et al. $ Amphisphaeriales, genera incertae sedis Amphisphaerella (Sacc.) Kirschst. Beltraniella Subram.$ Bleptosporium Steyaert Capsulospora K.D. Hyde$ Ceratosporium Schwein. Ceriophora Höhn. Ceriospora Niessl Chitonospora E. Bommer et al. Clypeophysalospora H.J. Swart Distorimula San Martín et al. Doliomyces Steyaert Dyrithium M.E. Barr Ellurema Nag Raj & W.B. Kendr. $ Flagellosphaeria Aptroot Frondispora K.D. Hyde Funiliomyces Aptroot$ Griphosphaerioma Höhn. Hyalotiopsis Punith. Immersidiscosia Kaz. Tanaka et al. $ Labridium Vestergr. Lindquistomyces Aramb. et al. Manokwaria K.D. Hyde Microdochium Syd. $ Monochaetinula Muthumary et al. Monographella Petr. $ Morinia Berl. & Bres. $ Fungal Diversity Mukhakesa Udaiyan & Hosag. Neobroomella Petr.# Ommatomyces Kohlm et al.# Paracainiella Lar.N. Vassiljeva Pemphidium Mont. Reticulosphaeria Sivan. & Bahekar Robillarda Sacc. $ Sporocadus Corda$ Synnemapestaloides T. Handa & Y. Harada Urosporella G.F. Atk. Urosporellopsis W.H. Hsieh et al. Xylochora Arx & E. Müll. Zetiasplozna Nag Raj$ Xylariales Nannf. Apiosporaceae K.D. Hyde et al. Appendicospora K.D. Hyde$ Arthrinium Kunze$ Dictyoarthrinium S. Hughes Endocalyx Berk. & Broome Scyphospora L.A. Kantsch. Spegazzinia Sacc. $ Cainiaceae J.C. Krug Arecophila K.D. Hyde$ Atrotorquata Kohlm. & Volkm.-Kohlm. $ Cainia Arx & E. Müll. $ Seynesia Sacc. $ Coniocessiaceae Asgari & Zare Coniocessia Dania García et al. $ Diatrypaceae Nitschke Anthostoma Nitschke$ Cryptosphaeria Ces & De Not. $ Cryptovalsa Ces & De Not. ex Fuckel$ Diatrype Fr. $ Diatrypella (Ces & De Not.) De Not. $ Diamantinia A.N. Mill. et al. $ Diatrypasimilis J.J. Zhou & Kohlm. $ Echinomyces Rappaz Eutypa Tul. & C. Tul. $ Eutypella (Nitschke) Sacc. $ Leptoperidia Rappaz Libertella Desm. Monosporascus Pollack & Uecker$ Pedumispora K.D. Hyde & E.B.G. Jones$ Peroneutypa Berl. $ Phaeoisaria Höhn. $ Quaternaria Tul. & C. Tul. Graphostromataceae M.E. Barr et al. Graphostroma Piroz. $ Hyponectriaceae Petr. Apiothyrium Petr. Arecomyces K.D. Hyde Arwidssonia B. Erikss. Cesatiella Sacc. Chamaeascus L. Holm et al. Charonectria Sacc. Discosphaerina Höhn. $ Exarmidium P. Karst. Frondicola K.D. Hyde Hyponectria Sacc. $ Micronectria Speg. Papilionovela Aptroot Pellucida Dulym. et al. Physalospora Niessl$ Phragmitensis M.K.M. Wong et al. Rhachidicola K.D. Hyde & J. Fröhl. Xenothecium Höhn. Iodosphaeriaceae O. Hilber Iodosphaeria Samuels$ Lopadostomaceae Daranagama & K.D. Hyde Creosphaeria Theiss. $ Lopadostoma (Nitschke) Traverso$ Melogrammataceae G. Winter Melogramma Fr. $ Pseudomassariaceae Senan., & K.D. Hyde Leiosphaerella Hohn.$ Pseudomassaria Jacz.$ Vialaeaceae P.F. Cannon Vialaea Sacc. $ Xylariaceae Tul. & C. Tul. “Hypoxyloideae” Annulohypoxylon Y.M. Ju et al. $ Anthocanalis Daranagama et al. $ Barrmaelia Rappaz$ Biscogniauxia Kuntze$ Calceomyces Udagawa & S Ueda$ Camillea Fr. $ Chlorostroma A.N. Mill. et al. Collodiscula I Hino & Katum. $ (cf.Stadler et al. 2013) Daldinia Ces. & De Not. $ Entonaema Möller$ Hypoxylon Bull. $ Nodulisporium Preuss$ Obolarina Pouzar$ Phylacia Lév. $ Pyrenomyxa Morgan$ Pyriformiascoma Daranagama et al. $ Rhopalostroma D. Hawksw. $ Rostrohypoxylon J Fourn & M Stadler$ Ruwenzoria J. Fourn. et al. $ Thamnomyces Ehrenb. $ Thuemenella Penz. & Sacc. $ Vivantia J.D. Rogers et al. “Xylarioideae” Amphirosellinia Y.M. Ju et al. $ Anthostomella Sacc. $ Appendixia B.S. Lu & K.D. Hyde Fungal Diversity Areolospora S.C. Jong & E.E. Davis Arthroxylaria Seifert & W Gams$ Ascotricha Berk. $ Astrocystis Berk. & Broome$ Brunneiperidium Daranagama et al. $ Cannonia J.E. Taylor & K.D. Hyde# Chaenocarpus Rebent.# Coniolariella Dania García et al. $ Cyanopulvis J. Fröhl. & K.D. Hyde Durotheca Læssøe et al. Emarcea Duong et al. $ Engleromyces Henn. Entoleuca Syd. $ Euepixylon Füisting$ Fasciatispora K.D. Hyde$ Gigantospora B.S. Lu & K.D. Hyde Guestia G.J.D. Sm. & K.D. Hyde$ Halorosellinia Whalley et al. $ Helicogermslita Lodha & D. Hawksw. Hypocopra (Fr) J. Kickx f$ Hypocreodendron Henn. Induratia Samuels et al. $ Jumillera J.D. Rogers et al. Kretzschmaria Fr. $ Kretzschmariella Viégas Leprieuria Laessøe et al. Leptomassaria Petr. Lunatiannulus Daranagama et al. $ Muscodor Worapong et al. $ Myconeesia Kirschst. Nemania Gray$ Nipicola K.D. Hyde Occultitheca J.D. Rogers & Y.M. Ju$ Ophiorosellinia J.D. Rogers et al. $ Pandanicola K.D. Hyde Paramphisphaeria F.A. Fernández et al. Paucithecium Lloyd Podosordaria Ellis. & Holw. $ Poroleprieuria M.C. González et al. Poronia Willd. $ Rosellinia De Not. $ Sabalicola K.D. Hyde Sarcoxylon Cooke Sclerodermatopsis Torrend Spirodecospora B.S. Lu et al. Steganopycnis Syd. & P Syd. Stilbohypoxylon Henn. $ Streblema Chevall. Striatodecospora D.Q. Zhou et al. $ Stromatoneurospora S.C. Jong & E.E. Davis$ Theissenia Maubl. $ Virgaria Nees$ Wawelia Namysl. Xylaria Hill ex Schrank$ Xylocrea Möller Xylotumulus J.D. Rogers et al. $ Xylariales, genera incertae sedis Adomia S. Schatz Ascotrichella Valldos. & Guarro Biporispora J.D. Rogers et al. Diamantinia A.N. Mill. et al. $ Fassia Dennis Lanceispora Nakagiri et al. $ Lasiobertia Sivan. $ Leiosphaerella Höhn.$ Linocarpon Syd. & P. Syd. $ Neolinocarpon K.D. Hyde$ Oxydothis Penz. & Sacc. $ Palmicola K.D. Hyde Pidoplitchkoviella Kiril. $ Plectosphaera Theiss. $ Polyancora Voglmayr & Yule$ Pulmosphaeria Joanne E. Taylor et al. $ Sporidesmina Subram. & Bhat$ Squamotubera Henn. Subramaniomyces Varghese & V.G. Rao$ Yuea O.E. Erikss. Xylariomycetidae genera incertae sedis Neohypodiscus J.D. Rogers et al.# Whalleya J.D. Rogers et al. $ The latest outline of Xylariomycetidae was published by Maharachchikumbura et al. (2015). However, the present study, Norphanphoun et al. (2015) and Li et al. (2015b) have revised the group. A new outline is therefore provided below. This should be regarded as a working outline as there are many genera that lack sequence data, others where the type species has not been sequenced, some with incomplete sequence data, and other genera with only one or two taxa sequenced. The subclass Xylariomycetidae was introduced by Eriksson and Winka (1997) and many species in the subclass have large stromata, containing numerous ascomata, asci with a J+ apical apparatus and brown unicellular ascospores (Maharachchikumbura et al. 2015). However, they may also have single ascomata, often immersed under a clypeus, and unicellular, apiosporous, trans-septate or muriform ascospores, ranging from hyaline to black (Zhang et al. 2006; Stadler et al. 2013). The subclass is presently rather confusing as many, especially the astromatic genera, lack sequence data (Maharachchikumbura et al. 2015). In addition, the connections of the sexual and asexual morphs have not been established for many of the stromatic genera, in particular in the Xylariaceae. Amphisphaeriales D. Hawksw. & O.E. Erikss., Syst. Ascom. 5 (1): 177 (1986) Fungal Diversity Facesoffungi number: FoF 00672 Saprobic, pathogenic or endophytic on plants, appearing as black spots on the host substrate, made up of fungal hyphae and host cells. Sexual morph: Pseudostromata or stromata if present, with immersed to erumpent, solitary, rarely aggregated, scattered, globose to subglobose, coriaceous, brown to black, ostiolate, ascomata with periphyses. Peridium with one or few layers, mostly comprising an outer layer of thickwalled, brown cells and inner layer of thick or thin-walled, hyaline cells. Hamathecium comprising septate, hyaline, paraphyses. Asci 8-spored, unitunicate, cylindrical, clavate or narrowly globose, short pedicellate, with J+ or J- apical ring. Ascospores uniseriate or multi-seriate, hyaline, light brown to dark brown, ellipsoid, apiosporous, fusiform or oval, unicellular to 4-septate smooth or ornamented, sometimes bearing hyaline, slender, unbranched appendages, sometimes with a gelatinous sheath. Asexual morph: Coelomycetous. Conidiomata stromatic, solitary to gregarious, immersed to erumpent, acervular, pycnidial to irregular, unilocular or irregularly plurilocular, dark brown to black. Peridium comprising brown cells of textura angularis. Conidiophores indistinct or if distinct, arising from the inner layer cells of basal stroma, sometimes reduced to conidiogenous cells, long, cylindrical, hyaline, septate and sometimes branched. Conidiogenous cells discrete, holoblastic, annelledic, indeterminate, integrated, cylindrical, ampulliform, or lageniform, smooth, hyaline, becoming pale brown with age. Conidia cylindrical, clavate, ellipsoid or fusiform, aseptate to 4-euseptate, hyaline, subhyaline, pale olivaceous or brown, bearing cellular, filiform or attenuated, sometimes only apical or having both apical and basal appendages. Notes: The order Amphisphaeriales was introduced by Eriksson and Hawksworth (1986) to accommodate fungal taxa in Amphisphaeriaceae, Cainiaceae, Clypeosphaeriaceae and Hyponectriaceae. Eriksson and H a w k s w o r t h ( 1 9 8 7 ) h o w e v e r, s y n o n y m i z e d t h e Amphisphaeriales under Xylariales and this has been followed by subsequent authors (Lumbsch and Huhndorf 2007, 2010). As a result of combined LSU and ITS gene sequence analysis in this study (Fig. 1), we resurrect the order Amphisphaeriales with the currently existing families Amphisphaeriaceae, Clypeosphaeriaceae and four novel families; Bartaliniaceae, Discosiaceae, Pestalotiopsidaceae and Phlogicylindriaceae. Members in this order show a wide variation in morphology. However the sexual morph of this order is characterized by immersed to erumpent, mostly solitary, globose to subglobose, coriaceous ascomata, with or without stromatic tissues, cylindrical or clavate asci, with J+ or Japical rings and ellipsoid, apiosporous, fusiform or oval, unicellular to trans-septate, variously coloured ascospores, with smooth or ornamented walls and with presence or absence of appendages. The asexual morphs have acervular or pycnidial conidiomata with holoblastic, annellidic, ampulliform, or lageniform, conidiogenous cells and aseptate to transeuseptate, variously coloured conidia, often bearing appendages. Amphisphaeriaceae G. Winter [as ‘Amphisphaerieae’], Rabenh. Krypt.-Fl., Edn 2 (Leipzig) 1.2: 259 (1885) Facesoffungi number: FoF 00673 Saprobic on branches and wood, appearing as slightly raised, black spots on host surface, often surrounded by black patches, herein termed the pseudostromata. Sexual morph: Pseudostromata when present made up of host cells and brown to black fungal hyphae. Ascomata scattered, solitary or clustered, immersed, globose to subglobose, coriaceous, dark brown, ostiolate, papillate. Papilla short, internally covered by hyaline, filamentous periphyses. Peridium two-layered, outer layer of thick-walled, brown cells of textura angularis, inner layer of small, thin-walled, hyaline cells of textura angularis. Hamathecium comprising numerous, septate, hyphae-like paraphyses. Asci 8-spored, unitunicate, cylindrical, short pedicellate, with J+ or J- apical ring. Ascospores uniseriate to overlapping uniseriate, light to dark brown, oval to fusiform, 1-septate, not or slightly constricted at the septum, smooth-walled, surrounded by mucilaginous sheath. Asexual morph: Coelomycetous. Conidiomata globose, dark brown. Conidiophores septate, branched, hyaline. Conidiogenesis cells elongate conical, septate, annelledic. Conidia 1-celled elongate-fusiform, hyaline. Type: Amphisphaeria Ces. & De Not., Comm. Soc. crittog. Ital. 1 (4): 223 (1863) Type species: Amphisphaeria umbrina (Fr.) De Not., Sfer. Ital.: 69 (1863) Notes: The family Amphisphaeriaceae was introduced by Winter (1884–1886) to accommodate Amphisphaeria which is typified by A. umbrina. This family is characterised by immersed or erumpent perithecial ascomata, some forming in pseudostromata, with papillate ostioles, unitunicate, cylindrical, asci with J- or J+ apical rings, and light to dark brown, 1-celled ascospores (Kang et al. 1999b). This family was recognized as a heterogeneous sexual morph family accommodating 36 genera (Hawksworth et al. 1995). Kang et al. (1998) restricted the family to ten genera based on phylogenetic analysis of ITS sequence data, often with pestalotiopsis-like asexual morphs. However, later the family was reduced to 9 genera (Kirk et al. 2001) excluding some genera to Cainiaceae, Clypeosphaeriaceae and Hyponectriaceae. In this study we exclude most genera and restrict the family to Amphisphaeria sensu stricto. Type: Amphisphaeria Ces. & De Not., Comm. Soc. crittog. Ital. 1 (4): 223 (1863) Saprobic on woody branches, appearing as slightly raised, black dots on host surface, often surrounded by black patches, the pseudostromata. Sexual morph: Pseudostromata when present made up of host cells and brown to black fungal hyphae. Ascomata perithecial, scattered, solitary or clustered, Fungal Diversity Fig. 2 Amphisphaeria sorbi (holotype) a Ascomata on substrate. b Cross section of ascoma. c Peridium. d Paraphyses. e, f Asci in water. g Asci in Melzer’s reagent. h, i Ascospores. j Sheath around ascospore. k Germinating ascospore. i Conidiomata on MEA. m–p Conidiophore and conidiogenous cells with attached conidia. q. Conidia. Scale bars: a=1 mm, b=100 μm, c=50 μm, e–g=20 μm, d, h–k=10 μm, l–q= 10 μm immersed, globose to subglobose, coriaceous, dark brown, ostiolate. papillate. Papilla short, narrow, conical, periphysate. Periphyses hyaline, filamentous. Peridium two-layered, outer layer comprising dark brown cells arranged in a textura angularis and inner layer comprising thin-walled, hyaline cells. Hamathecium comprising numerous, filamentous, Fungal Diversity Fungal Diversity Bartalinia robillardoides (MFLU 13–0084). a Conidiomata on leaf of Eucalyptus sp. b c Cross section of conidioma on leaf. d, f Developing conidia attached to conidiogenous cells. e, f Cross section of conidioma on PDA. g–l. Conidia. j. Culture on PDA. k. Germinating conidium. Scale bars: b–e=150 μm, d–f=20 μm, g–i, l=25 μm Fig. 3 septate, slightly tapering paraphyses. Asci 8-spored, unitunicate, cylindrical, indistinctly pedicellate, apex rounded, with J+ or J- apical ring. Ascospores uniseriate to overlapping uniseriate, light brown to dark brown, oval to fusiform, 1septate, not or slightly constricted at the septum, smoothwalled or ornamented, sometimes with a mucilaginous sheath. Asexual morph: Coelomycetous. Conidiomata solitary or aggregated, globose, dark brown. Peridium comprising of thickwalled, septate, brown mycelium. Conidiophores septate, branched, thick-walled, hyaline. Conidiogenous cells elongate conical, thin-walled, septate, hyaline, annelledic. Conidia hyaline, elongate-fusiform, 1-celled, smooth-walled. Notes: Amphisphaeria was introduced by Cesati and De Notaris (1863) without designating a generic type (Wang et al. 2004). Petrak (1923) proposed A. umbrina as the lectotype of the genus which was introduced earlier as Sphaeria umbrina. A description of A. umbrina was provided by Hyde et al. (1996). However this was not based on the type specimen. Hence, Hyde et al. (1996) epitypified a collection listed by Cesati and De Notaris (1863) as S. umbrina and also used a specimen used by Petrak (1923) to describe A. umbrina (Wang et al. 2004). The epitype of A. umbrina is illustrated in Wang et al. (2004). Amphisphaeria umbrina has a distinct clypeus comprising outer, brown and inner, hyaline cells of textura angularis, cylindrical asci, with a J , discoid, subapical ring and ellipsoidal, light brown, one-median septate ascospores. Wang et al. (2004) accepted 12 species in the genus. Four species of Amphisphaeria have J-, apical rings (Wang et al. 2004; Liu et al. 2015). In this study, we illustrate the sexual and asexual morph of Amphisphaeria sorbi, the latter being introduced in Lui et al. (2015). Amphisphaeria sorbi Senan. & K.D. Hyde, in Liu et al., Fungal Divers 71: 10 (2015), Fig. 2 Notes: This species was introduced in Liu et al. (2015) and is similar to A. umbrina. As this species formed an asexual morph in culture we illustrate both sexual and asexual morphs here (Fig. 2). Material examined: ITALY, Trento [TN], Dimaro, Folgarida, on branch of Sorbus aucuparia L. (Rosaceae), 2 August 2013, E. Camporesi, IT 1400 (MFLU 14–0797, holotype). Bartaliniaceae Wijayawardene, Maharachch. & K.D. Hyde, fam. nov. Index Fungorum number: IF511183; Facesoffungi number: FoF 00667 Saprobic or rarely pathogenic causing leaf spots, on various hosts. Sexual morph: Pseudostromata immersed, solitary, scattered. Ascomata immersed to semi-immersed, solitary to gregarious, globose to subglobose, black. Ostiole papillate, central, circular, periphysate. Peridium composed of brown to dark brown cells of textura angularis or textura prismatica, with thin inner layer composed of thin-walled, hyaline cells of textura angularis. Hamathecium comprising filiform, septate, hyaline, smooth-walled paraphyses. Asci 8spored, unitunicate, cylindrical, short pedicellate, apically rounded a with J- or J+ subapical ring. Ascospores overlapping uniseriate, pale brown to brown, ellipsoid to fusiform, 3– 4-septate, smooth-walled. Asexual morph: Coelomycetous. Conidiomata acervular or pycnidial to irregular, solitary to gregarious, superficial to sub-immersed, unilocular, globose to subglobose, dark brown to black. Ostiole apapillate. Conidiomata wall comprising two strata, inner wall composed of thick-walled, dark brown cells of textura angularis, inner wall thin, composed of hyaline to sub-hyaline cells of textura an gula ris. Co nidio pho re s pr esen t or redu ced to conidiogenous cells; when present cylindrical, hyaline, sparsely septate, smooth-walled. Conidiogenous cells holoblastic, ampulliform, integrated or discrete, determinate, hyaline, smooth-walled. Conidia fusiform, straight to slightly curved, subhyaline to brown, bearing only apical appendages or having both apical and basal appendages. Type: Bartalinia Tassi, Bulletin Labor. Orto Bot. de R. Univ. Siena 3: 4 (1900) Notes: In the molecular analyses (Fig. 1), Bartalinia sensu stricto groups with Broomella, Hyalotiella and Truncatella and forms a distinct clade in Amphisphaeriales. Further, the Bartalinia clade comprises a newly introduced genus viz. Zetiasplozna. This clade is distinct from the other families in Amphisphariales and thus we introduce a new family Bartaliniaceae. The sexual morph genera in Bartaliniaceae are characterised by immersed to semi-immersed ascomata with papillate ostioles, unitunicate, cylindrical asci, with J- or J+ apical rings, and light to dark brown, 3–4-septate ascospores. The asexual typified genera have superficial to subimmersed acervular or pycnidial to irregular fruiting bodies, apapillate ostioles, conidiophores reduced to conidiogenous cells, holoblastic conidiogenesis and fusiform, straight to slightly curved, subhyaline to brown conidia with apical or apical and basal appendages. Bartalinia Tassi, Bulletin Labor. Orto Bot. de R. Univ. Siena 3: 4 (1900) Facesoffungi number: FoF 00659 Endophytic or saprobic on substrates of a range of plant hosts. Sexual morph: Undetermined. Asexual morph: Conidiomata pycnidial or variable, solitary to gregarious, subepidermal, erumpent at maturity, globose, unilocular, brown to black. Conidiomata wall two layered, outer layer comprising dark brown to black cells of textura angularis, inner wall comprising thin, hyaline cells of textura angularis. Fungal Diversity C o n i d i o p h o re s r e d u c e d t o c o n i d i o g e n o u s c e l l s . Conidiogenous cells ampulliform, holoblastic, discrete, determinate, hyaline, generated from inner layer of the pycnidium wall. Conidia hyaline to pale brown, subcylindrical, trans-septate, with appendages, basal cell with truncate base, obconic, hyaline, with single unbranched appendage; 3 median cells subcylindrical, hyaline to pale brown, apical cell conical, almost hyaline, with threebranched appendage. Type species: Bartalinia robillardoides Tassi, Bulletin Labor. Orto Bot. de R. Univ. Siena 3: 4 (1900) Notes: Tassi (1900) established Bartalinia, with B. robillardoides Tassi as the type species and currently, the genus comprises of 19 species epithets (Index Fungorum 2015). Morgan-Jones et al. (1972) accepted only nine species under Bartalinia and Sutton (1980) also accepted the same number. Sutton (1980) mentioned that the genus needs taxonomic revision. Von Arx (1981) reduced Bartalinia under Seimatosporium Corda, but this was rejected by Nag Raj (1993). Further, Nag Raj (1993) accepted only six species (including the type species), viz. Bartalinia bischofiae Nag Raj, B. lateripes (Ellis & Everh.) Nag Raj, B. laurina (Mont.) Nag Raj, B. pistacina (J.L. Maas) Nag Raj and B. tamarindi Nag Raj. Five new species have been described since the revision of Nag Raj (1993) viz. Bartalinia ananatis Li Zeng et al. (Chi et al. 2002), B. dracaenae P.G. Xi et al. (Xi et al. 2000), B. goniolimonis Andrian. & Minter (Andrianova and Minter 2007), B. mellea F. Anderson & Bianchin. (Anderson and Bianchinotti 1996) and B. pondoensis Marincowitz et al. (Marincowitz et al. 2010). Bartalinia robillardoides Tassi, Bulletin Labor. Orto Bot. de R. Univ. Siena 3: 4 (1900) ≡ Seimatosporium robillardoides (Tassi) Arx, Gen. Fungi Sporul. Cult., Edn 3 (Vaduz): 224 (1981) Facesoffungi number; F0F 00660; Fig. 3 Saprobic on leaf, stems and branches of a range of host plants. Sexual morph: Undetermined. Asexual morph: Conidiomata 170–240μm diam., 150–200μm high, pycnidial or variable, solitary to gregarious, of subepidermal in origin, then erumpent, globose, unilocular, brown to black, with apapillate ostiole. Pycnidial wall of two layers, outer layer comprising dark brown to black cells of textura angularis, inner wall thin, hyaline. Conidiophores reduced to conidiogenous cells. Conidiogenous cells 2–7 × 2–3 μm, ampulliform, holoblastic, integrated, determinate, hyaline, smooth, generated from innermost layer of the pycnidium wall. Conidia 20–30×3–4μm (x =22.52×3.21μm, n=20), hyaline to pale brown, subcylindrical, 4-septate, slightly constricted at the septa, smooth-walled, with appendages, basal cell hyaline, with a truncate base, obconic, 2–4μm long, baring an unbranched single appendage; 3 median cells subcylindrical, hyaline to pale brown, 15–22 μm long (altogether); apical cell conical, almost hyaline, 2–4μm long, with unbranched appendage, attenuate, flexuous, 15–20μm, tri-radiate. Culture characteristics: On PDA greyish brown, not zonate, fast growing, attaining 3 cm in 3 days at 25 °C, comprising circular, flat, thin mycelium. Material examined: THAILAND, Chiang Rai, Mae Fah Luang University grounds, on leaves of Eucalyptus sp., 30 June 2012, Nalin N. Wijayawardene, NNW 120630–1 (MFLU 13–0084), living culture, MFLUCC 12–0070, GUCC T70 Notes: Our collection on leaves of Eucalyptus spp. morphologically resembles Bartalinia robillardoides (Nag Raj 1993; Crous et al. 2014a). Further, molecular data also showed that the new strain resides in Bartalinia sensu stricto with B. robillardoides (Fig. 3). Crous et al. (2014a) designated the lectotype and epitype of B. robillardoides as MBT178268 and CBS H-21728, respectively. Broomella Sacc., Syll. fung. (Abellini) 2: 557 (1883) = Keissleria Höhn., Annls mycol. 16 (1/2): 93 (1918) Facesoffungi number: FoF 00626 Saprobic on various hosts. Sexual morph: Ascomata solitary to gregarious, uniloculate, glabrous, globose to subglobose, papillate. Ostiole centrally located, composed of longitudinally aligned cells, and internally lined with hyaline periphyses. Peridium composed of light yellow, thick-walled cells of textura prismatica in the upper part and surrounding the ostiole, and thin-walled, hyaline to pale brown cells in other parts. Hamathecium comprising numerous, cylindrical, hypha-like, septate, paraphyses tapering towards the ends. Asci 8-spored, unitunicate, cylindrical to cylindrical-clavate, pedicellate, apically rounded, with a J- apical ring. Ascospores biseriate or overlapping bi- or tri-seriate, pale greyish brown, or brown, with ends cells yellowish to pale grayish brown, fusiform, glabrous, straight or inequilaterally curved, 3septate, constricted at the septa, thick-walled, with doliform median cells, end cells conical, each bearing an unbranched, terminal tubular appendage. Asexual morph: Coelomycetous. Conidiomata stromatic, pycnidioid, scattered to gregarious, immersed to semi-immersed, rounded, oval or elongated, black, unilocular, papillate, glabrous. Pycnidial wall comprising thick-walled cells of textura globulosa to textura angularis, outwardly pale brown to brown, inwardly merging with relatively thin-walled and colourless cells. Conidiophores reduced to conidiogenous cells. Conidiogenous cells integrated, cylindrical, phialidic, percurrently proliferating 1–2-times, hyaline, smooth. Conidia pale brown or brown, fusiform to aciculate, with acute ends, straight or slightly curved, 3-septate, constricted at septa, verruculose, thick-walled, bearing a single tubular appendage at each end, or 2–5-appendage at apex and a single appendage at the base. Type species: Broomella vitalbae (Berk. & Broome) Sacc., Syll. fung. (Abellini) 2: 558 (1883) Fungal Diversity ≡ Hypocrea vitalbae Berk. & Broome, Ann. Mag. nat. Hist., Ser. 33: 363 (1859) Facesoffungi number: FoF 00589, Fig. 4 Notes: Broomella was introduced by Saccardo (1883) based on B. vitalbae (Berk. & Broome) Sacc. The genus is characterized by unitunicate, cylindrical-elongate asci, with a Fig. 4 Broomella vitalbae (epitype) a, b Black ascomata on the host. c Section of peridium. d, e Vertical section of ascomata. f Ostiole. g Paraphyses. h–k Asci with ascospores. l–o Ascospores. p–q Black conidiomata on the host. r Vertical section of conidiomata. s Conidiophores, conidiogenous cells and developing conidia. t–v Conidia. Scale bars: a=500 μm, b=200 μm, c=20 μm, d–e=100 μm, f=20 μm, g–o =10 μm, p=200 μm, q=500 μm, r=100 μm, s–v=10 μm Fungal Diversity J-, discoid apical ring, and 3-septate, ellipsoid-fusiform ascospores, with brown median cells, lighter terminal cells, and single, centric appendages arising from the ends (Shoemaker and Müller 1963; Li et al. 2015a). Broomella has been shown to be linked to a pestalotiod-like asexual morph (Shoemaker et al. 1989; Yuan and Zhao 1992; Kang et al. 1999a). Shoemaker and Müller (1963) introduced B. acuta Shoemaker & E. Müll. and B. excelsa Shoemaker & E. Müll. in the genus. There are 20 species epithets listed under Broomella in Index Fungorum (2015), however, none of these had been studied using sequence data. The Broomella species and their truncatella-like asexual morphs differ in various ways from the type species of Broomella and its asexual morph and are probably not congeneric. Through a study of LSU sequence data and together with existing morphological data, Li et al. (2015a) showed that Broomella appears to be a distinct, natural group in Amphisphaeriaceae and the asexual morph have conidia with a single apical and basal appendages. Materials examined: ITALY. Province of Forlì-Cesena [FC], Modigliana, Montebello, on dead stem of Clematis vitalba, 23 February 2013, Erio Camporesi, IT-1079 (MFLU 15–0065, epitype); ex-epitype living culture, MFLUCC 13– 0 79 8 , I C MP ; I TA LY. P r o vi nc e o f A r e z zo [ A R ] , Montemezzano, on dead stem of Clematis vitalba, 25 August 2013, Erio Camporesi, IT-1430 (MFLU 15–0054); living culture, MFLUCC 14–1000. Hyalotiella Papendorf, Trans. Br. mycol. Soc. 50 (1): 69 (1967) Index Fungorum number: IF 8588 Facesoffunginumber: FoF00627 Saprobic on various hosts. Sexual morph: Ascomata perithecial, immersed, becoming erumpent, solitary, gregarious or confluent, globose to subglobose, blackened, glabrous, papillate. Ostiole lined with filiform, hyaline, periphyses. Peridium composed of outer, dark brown, thick-walled cells of textura angularis and thin-walled, hyaline cells in the inner layers. Hamathecium comprising filiform, hyaline, paraphyses, evanescent at maturity. Asci 8-spored, unitunicate, cylindrical to clavate, short pedicellate, apex rounded, with a J+ subapical ring. Ascospores overlapping uniseriate, fusiform to ellipsoidal with obtuse apex, and with narrow base, initially hyaline, and becoming brown at maturity, mostly 1-septate, occasionally 2–3-septate, constricted at septa, straight or slightly curved, thick-walled, smooth. Asexual morph: Coelomycetous. Conidiomata stromatic, pycnidial, vaseshaped, scattered to gregarious, immersed or semi-immersed, globose to subglobose, unilocular, glabrous, brown to dark brown, ostiolate. Ostiole lageniform, single, centrally located, with a well-developed neck, thick-walled. Peridium thickwalled cells of textura angularis, brown to dark brown. Conidiophores arising all around the cavity of the conidioma, short, often reduced to conidiogenous cells. Conidiogenous cells integrated, cylindrical, long, phialidic, hyaline, smooth. Conidia cylindrical to fusiform, with an obtuse apex and a narrow truncate base, straight or slightly curved, 3-septate, pale brown to brown except for the hyaline apical cell, median cells longer than ends cells, bearing 2–5-branched, filiform, flexuous, hyaline appendages arising from apical cell. Type species: Hyalotiella transvalensis Papendorf, Trans. Br. mycol. Soc. 50 (1): 69 (1967). Notes: Hyalotiella was introduced by Papendorf and DuToit (1967) with H. transvalensis Papendorf as type species. The genus is characterized by having vase-shaped pycnidia, with a long neck, and 3-septate, cylindrical, pale brown to brown conidia, with median cells that are longer than the ends cells, and 2–5-branched, filiform, apical appendages (Nag Raj 1993). According to the Index Fungorum (2015) there are four epithets in Hyalotiella. However, H. subramanianii was transferred to Hyalotiopsis by Nag Raj (1993) based on conidial characters and Hyalotiella. orientalis was synonymised under Hyalotiella americana (Speg.) Nag Raj (Nag Raj 1993). Li et al. (2015a) introduced an additional taxon in Hyalotiella, namely, H. spartii W.J. Li et al. based on morphological characters, while they also provided a sequence for this species. Thus, to date, four species are accepted in Hyalotiella, viz. H. americana, H. spartii W.J. Li, Camporesi & K.D. Hyde, H. transvalensis and the sexual species H. rubi described in this paper. The Dyrithiopsis-like sexual morph of Hyalotiella has not been previously reported (Sutton 1980; Nag Raj 1993). Hyalotiella rubi shows somewhat similar morphological characters (i.e., form of ascomata and ascospores) with the type species of Dyrithiopsis, viz. Dyrithiopsis lakefuxianensis L. Cai et al., but it is phylogenetically distinct from D. lakefuxianensis (Fig. 1) the latter has a Monochaetiopsis conidial state (Jeewon et al. 2003a, b). Hyalotiella spartii W.J. Li, Camporesi & K.D. Hyde Cryptog., Mycol. 36 (1): 93–108 (2015). Index Fungorum number: IF551047 Facesoffunginumber: FoF00590; Fig. 5 Saprobic on dead stems of Spartium junceum, forming conspicuous rounded, black conidiomata. Sexual morph: Undete rmin ed. Asex ual mor ph: co elom yce to us. Conidiomata 250–300 μm high, 200–250 μm diam., stromatic, pycnidial, vase-shaped, scattered to gregarious, epidermal to subepidermal in origin, globose to subglobose, semi-immersed, unilocular with the locule occasionally convoluted or irregularly divided, glabrous, brown to dark brown, ostiolate. Ostiole lageniform, single, centrally located, with a well-developed neck, thick-walled. Peridium 30–50μm wide, composed of 7–8-cell layers, with thick-walled cells of textura angularis, brown to dark brown. Conidiophores arising all around the cavity of the conidioma, short, often reduced to conidiogenous cells. Conidiogenous cells 10–15μm wide, integrated, cylindrical, long, phialidic, hyaline, smooth. Conidia Fungal Diversity Fig. 5 Hyalotiella spartii (holotype) a Herbarium specimen. b, c Black coniodiomata on the host. d Vertical section of conidioma. e Ostiole. f, h–j Conidiogenous cells and developing conidia. g Section of pycnidial wall. k–n Conidia. Scale bars: c=200 μm, d=100 μm, e=50 μm, f, h–j=10 μm, g=10 μm, k–n=10 μm Fungal Diversity 25–28×3–4μm (x =26×3.5μm; n=20), fusiform, 3-septate, bearing apical appendages; basal cell obconic with an obtuse base, almost colourless, with 2 cylindrical to subcylindrical, thick-walled, yellowish to brown, median cells constricted at septa, and with conic, thin-walled, hyaline apical cell, with apical appendages 14–20μm long, tubular, acellular, often irregularly or dischotomously branched at the base, filiform, flexuous, hyaline. Materials examined: ITALY. Province of Forlì-Cesena [FC], Santa Sofia, Collina di Pondo, on dead stem of Spartium junceum, 16 October 2012, Erio Camporesi, IT 812 (MFLU 15–0055 holotype); ex-type living culture, MFLUCC 13–0397=ICMP 20788; ibid., IT-812B (MFLU 15–0066); living culture MFLUCC 15–0024=ICMP. Hyalotiella rubi Senan., Camporesi & K. D. Hyde, sp. nov. Index Fungorum Number: IF551189; Facesoffungi number: FoF 00680; Fig. 6 Etymology: Name referring to the host genus on which the species occurs. Holotype: MFLU 15–0740 Saprobic on stems of Rubus sp., appearing as shiny, black, raised areas on substrate. Sexual morph: Pseudostromata made up of host cells and black fungal hyphae, forming a wide, darkened layer around the ostiole, and a thin, darkened, hyphal layer beneath the cuticle, spreading on the host surface to form a darkened region above the ascomata. 140–195μm high, 200–245μm diam., (x =165×220μm, n=20), perithecial, immersed, becoming erumpent, scattered, solitary or aggregated, globose to subglobose, coriaceous, with very short papillate ostiole, ostiolar canal lined with filiform, hyaline, periphyses. Peridium 10–20μm wide (x =16μm, n=20), comprising two strata, outer stratum comprising 7–10 layers of compressed, thick-walled cells of textura angularis and inner stratum comprising 1–2 layers of hyaline, thin-walled, large, cells of textura angularis. Hamathecium comprising 2–5μm wide (x =3μm, n=20), filiform, septate, hyaline paraphyses, evanescent at maturity. Asci 80–100×8–10.5μm (x =90× 9.5 μm, n = 20), 8-spored, unitunicate, cylindrical, short Fig. 6 Hyalotiella rubi (holotype) a Appearance of ascomata on host substrate. b Cross section of ascomata. c Peridium. d Paraphyses. e–g Asci. h Asci in Melzer’s reagent showing J+, apical ring. i–n Ascospores. o Germinating ascospores. Scale bars: a=1 mm, b=50 μm, c–o=10 μm Fungal Diversity pedicellate, apically rounded, with a J+ subapical ring. Ascospores 12–15μm×5.5–7.5μm (x =13.5×6.5μm, n=20), overlapping uniseriate, initially hyaline and becoming brown at maturity, ellipsoidal to fusiform with blunt ends, mostly 1septate, rarely 2–3-septate, slightly constricted at the septa, lowest cell longer than upper cell, smooth-walled. Asexual morph: Undetermined. Culture characters: growing on MEA reaching 2 cm within 7 days when incubated at 18 °C. Flat, circular, white colony with serrate margin and white woolly centre. Materials examined: ITALY, Province of Forlì-Cesena [FC], Premilcuore, Fiumicello, on dead branch of Rubus sp., 24 April 2013, Nello Camporesi, IT 1197 (MFLU 15–0740, holotype); Ibid., (MFLU 15–0740bis, isotype); ibid., (MFLU 15–0740tris, paratype); ex-type living cultures, MFLUCC 13–0660=ICMP. Notes: Hyalotiella rubi is introduced as a new species in the family Bartaliniaceae based on the phylogenetic analysis of combined LSU and ITS sequence data. This is the first record of a sexual morph of Hyalotiella. However Hyalotiella rubi shows somewhat similar morphological characters to species of Dyrithiopsis. Phylogenetically they are not closely related. In the combined analyses of LSU and ITS sequence data of amphisphaeriaceous and xylariaceous taxa (Fig. 1), H. rubi shows a close affinity with H. spartii. Truncatella Steyaert Bull. Jard. bot. État Brux. 19: 293 (1949) Facesoffungi number: FoF 00705. Saprobic or mostly pathogenic on plant leaves and twigs. Sexual morph: Ascomata solitary or gregarious, scattered, immersed under minute clypeus, uniloculate, globose to subglobose, coriaceous, papillate or apapillate, brown. Ostiole central, wide, rounded. Papilla composed of cells of textura porrecta and internally lined with hyaline periphyses. Peridium comprising light yellow or brown, thick-walled cells of textura prismatica in the upper part, and thick-walled, hyaline to pale brown cells of textura angularis in other parts. Hamathecium comprising numerous, hypha-like, septate, paraphyses, slightly constricted at the septa, tapering towards the ends. Asci 8-spored, unitunicate, cylindrical to cylindrical-clavate, pedicellate, apically rounded, with a J- apical ring. Ascospores biseriate or overlapping tri-seriate, hyaline when young, sometimes pale greyish-brown with doliform median cells and yellowish to pale greyish-brown end cells or brown at maturity, fusiform, glabrous, thick-walled, straight or inequilaterally curved, with pointed ends, 1–3-septate with constrictions at the septa and bearing unbranched, terminal appendages or ornamented wall. Asexual morph: Coelomycetous. Conidiomata acervular, immersed to semiimmersed, solitary to aggregated. Peridium comprising brown, thin-walled cells of textura angularis. Conidiophores hyaline, branched, septate, cylindrical. Conidiogenous cells holoblastic, indeterminate, integrated, cylindrical, hyaline, smooth, with several percurrent proliferations. Conidia with brown median cells and hyaline apical and basal cells, fusiform, straight, 3-euseptate, constricted at the septa, sometimes with a simple or rarely branched, cellular appendage, conical at apical cell, hyaline, with 1 or more apical, simple or branched tubular appendages, smooth-walled, thick-walled,. Type species: Truncatella truncata (Lév.) Steyaert, Bull. Jard. bot. État Brux. 19: 295 (1949) Notes: Truncatella introduced by Steyaert (1949) and is typified by T. truncata. The genus is typically associated with plants as an endophyte or pathogen (Shoemaker et al. 1989). According to molecular studies, Truncatella is paraphyletic with Bartalinia sharing a common ancestor (Jeewon et al. 2002; Li et al. 2015a). However, Truncatella may possibly be two genera. Truncatella vitalbae (Shoemaker & E. Müll.) Shoemaker et al., T. excelsa (Petr.) Shoemaker et al., and T. pestalozzioides (Dearn. & Fairm.) Shoemaker et al. were reported as the sexual morph of Broomella (Shoemaker et al. 1989). Li et al. (2015a) however, showed through molecular data that another coelomycetous species is the asexual morph of B. vitalbae. Hence the sexual morph of Truncatella still remains unresolved. Here we illustrate the sexual morph of a new Truncatella species as Truncatella spartii. Truncatella spartii Senan., Camporesi & K. D. Hyde, sp. nov. Fig. 6 Index Fungorum number: IF551196; Facesoffungi number: FoF 00685; Figs. 7 and 8 Etymology: Name referring to the host genus on which the species occurs. Holotype: MFLU 15–0721 Saprobic on stems of Spartium junceum L., appearing as black, circular, flat dots on substrate. Sexual morph: Ascomata 115–140μm high, 175–190μm diam., (x =135× 180μm, n=20), scattered, solitary, immersed, globose to subglobose, brown, ostiolate, apapillate. Ostiole wide, circular, even with host epidermis. Peridium 20–24μm wide near to ostiole (x =22.5μm, n=20), 6.5–13μm wide at the base (x = 9.5 μm, n =20), outwardly comprising 5–7 layers of brown cells of textura angularis, inwardly comprising 4–6 layers of thick-walled, hyaline cells of textura angularis. Hamathecium comprising 2.5–4μm wide (x =3μm, n=20), branched, septate, paraphyses, slightly constricted at the septa. Asci 62–70 ×6–6.5 μm (x = 65× 6.1 μm, n= 20), 8-spored, unitunicate, cylindrical, short pedicellate, apex slightly pointed, with an indistinct, J-, apical apparatus. Ascospores 8– 11.5×3.5–4μm (x =9.5×3.6μm, n=20), uniseriate to overlapping uniseriate, hyaline when young, brown at maturity, fusiform, with one thick septum, constricted at septum, pointed at ends. Asexual morph: Coelomycetous, Conidiomata 125– 155μm high, 145–180μm diam., (x =140×165μm, n=20), pycnidial, immersed, g lobose, brown, o stiolate. Conidiophores 3–5μm high, 3.5–4.5 μm diam. (x =3.5× 4 μm, n = 20), hyaline, simple or branched, cylindrical, Fungal Diversity Fig. 7 Truncatella spartii (holotype). a Ascomata on host. b Cross section of ascoma. c Peridium. d Paraphyses. e–i Asci. j–o Ascospores. Scale bars: a=1 mm, b=50 μm, c, d, j–o=10 μm, e–i=20 μm aseptate. Conidiogenous cells 8–17μm long, 8–12μm wide, (x =11.5×10.7μm, n=20), hyaline, cylindrical, annellidic. Conidia 8–12×4–5.5μm (x =10×5μm, n=20), clavate, 3septate, constricted at the septa, central cells large, upper central cell slightly larger than lower central cell, light brown, thick-walled, basal cell conical, hyaline, narrowing towards the base, apical cell small, hyaline, conical, bearing appendages. Appendages 11–16μm long (x =14.5μm, n=20), 1–3, thin, slender, hyaline, of equal length to that of the conidia. Culture characters: Growing on MEA reaching 1 cm within 7 days at 18 °C, fast growing, flat, colony circular, with serrate margin, sparse, white, aerial mycelia in the centre, with unbranched, hyaline, mycelia at the margin. Materials examined: ITALY, Province of Forlì-Cesena [FC], Galeata, Passo delle Forche, on branch of Spartium junceum L. (Fabaceae), 9 November 2013, Erio Camporesi, IT 1511 (MFLU 15–0721, holotype); ibid., (MFLU 15– 0721bis, paratype); living cultures, MFLUCC 15–0537 (from conidiomata), ICMP. Notes: In this study we introduced a new species on Spartium from Italy as Truncatella spartii. Megablast search results of T. spartii in GenBank give Truncatella helichrysi (Severini) B. Sutton and T. hartigii (Tubeuf) Steyaert. as close matches, however, it differs from them in having conical, hyaline apical cells, bearing 2–3 long, unbranched, hyaline conidial appendages. We observed Fungal Diversity Fig. 8 Truncatella spartii (holotype). a Herbarium specimens. b, c Conidiomata on host. d Cross section of conidioma e–f Conidia attached to conidiogenous cells. g–l Conidia. Scale bars: b=1 mm, c=200 μm, d=50 μm, e–i=20 μm ascomata associated with conidiomata on the host and DNA extraction was made directly from the ascomata by picking up the inner contents using fine forceps and checking under a compound microscope. Sequence data from both the sexual and asexual morphs showed them to be Truncatella. Clypeosphaeriaceae G. Winter [as ‘Clypeosphaerieae’], Rabenh. Krypt.-Fl., Edn 2 (Leipzig) 1.2: 554 (1886) Facesoffungi number: FoF 00678 Hemibiotrophic or saprobic on woody or herbaceous plants. Sexual morph: Pseudoclypeus comprising both host and fungal tissues, black. Ascomata immersed to erumpent, rarely superficial, solitary or aggregated, globose to subglobose, coriaceous, brown to black, ostiolate, papillate. Papilla short, narrow, internally lined with hyaline, filamentous periphyses. Peridium comprising dark brown to light brown, thick-walled cells of textura angularis, inwardly hyaline. Hamathecium comprising numerous, hypha-like, septate, flexuose, paraphyses, embedded in a gelatinous matrix. Asci 8-spored, unitunicate, cylindrical to broadly cylindrical, pedicellate, with a wedge-shaped, J-, or J+ apical ring. Ascospores uniseriate to biseriate, hyaline to brown, ellipsoidal to fusiform, sometimes oval, straight or curved, unicellular or septate, wall smooth or ornamented or striate, sometimes with sheaths, appendages, rarely with germ slits or germ pores. Asexual morph: Undetermined. Notes: The family Clypeosphaeriaceae was introduced by Winter (1884–1886) to accommodate Anthostomella, Clypeosphaeria, Hypospila, Linospora and Trabutia. Barr (1989) however, revisited the family and excluded all genera retaining Clypeosphaeria as the type of this monotypic family. Hawksworth et al. (1995) treated this family with Apiorhynchostoma, Ceratostomella, Clypeosphaeria, Crassoascus, Duradens, Frondicola, Jobellisia, Melomastia and Pseudovalsaria (Kang et al. 1999a; Smith et al. 2003). Lumbsch and Huhndorf (2010) accepted only Apiorhynchostoma, Aquasphaeria, Brunneiapiospora, Clypeosphaeria, Crassoascus, Duradens, Palmomyces and Pseudovalsaria in the family. Maharachchikumbura et al. (2015) accepted Apioclypea, Aquasphaeria, Brunneiapiospora, Clypeosphaeria, Crassoascus and Palmomyces as the genera in Clypeosphaeriaceae, following Lumbsch and Huhndorf (2010), as these genera, except for Clypeosphaeria, lack sequence data. Type: Clypeosphaeria Fuckel, Jb. nassau. Ver. Naturk. 23– 24: 117 (1870) [1869–70] Fungal Diversity Type species: Clypeosphaeria mamillana (Fr.) Lambotte, Flore Mycologique Belge 2: 247 (1880) Clypeosphaeria mamillana (Fr.) Lambotte, Flore Mycologique Belge 2: 247 (1880) Basionym: Clypeosphaeria notarisii Fuckel, Jb. nassau. Ver. Naturk. 23–24: 117 (1870). Facesoffungi number: FoF 00709; Fig. 9 Saprobic on decaying wood. Sexual morph: Clypeus dome-shaped, well-developed, black. Ascomata 380–450μm diam., 350–400μm high (x =428×389μm, n=10), immersed under the clypeus, solitary, scattered, globose to subglobose, coriaceous, black, ostiole central, papillate. Papilla short, narrow, internally lined with hyaline, filamentous periphyses. Peridium 20–35μm wide (x =25μm, n=20), comprising outer, brown, elongated, flat, cells of textura angularis and inner, hyaline, elongated, flat, cells of textura angularis. Hamathecium comprising numerous, 3–5 μm wide (x = 3.5μm, n=20), hypha-like, aseptate, unbranched, flexuose, paraphyses, tapering towards the apex. Asci 165–230×8– 11μm (x =198×10μm, n=20), 8-spored, unitunicate, cylindrical, short pedicellate, apex rounded, with 1.5–2.5μm diam., 2.5–3.5μm high (x =2.1×2.8μm, n=20), wedge-shaped, J+ apical apparatus. Ascospores 20–25×6–9μm (x =20×7μm, n=30), uniseriate, dark brown, ellipsoidal to fusiform, unicellular, straight or slightly curved, 0–5-distoseptate, not constricted at the septa, smooth-walled. Asexual morph: Undetermined. Material examined: GERMANY, Ahlen, Oestricher forest, December 1823, K.W.G.L Fuckel (G00127177, holotype). Notes: Clypeosphaeria is typified by Clypeosphaeria mamillana (Fr.) Lambotte (Kang et al. 1999a). This genus has been synonymized under several genera (Barr 1989), while Hyde et al. (1998) treated Clypeosphaeria as a monotypic genus, excluding all the other species. However currently 41 species have been listed under genus Clypeosphaeria (Mycobank 2015). Clypeosphaeria is characterized by 0–5pseudoseptate ascospores, without germ pores. The type species Clypeosphaeria mamillana is previously named as Clypeosphaeria notarisii Fuckel (Kang et al. 1999a; Mycobank 2015). However Clypeosphaeria notarisii was an illegitimate name, because the name should be a combination with Sphaeria clypeiformis De Not. (Mycobank 2015). Discosiaceae Maharachch. & K.D. Hyde, fam. nov. Index Fungorum number IF551177; Facesoffungi number: 0665 Saprobic or pathogenic on plant leaves. Sexual morph: Ascomata scattered, immersed to erumpent. Ostiole circular, papillate. Peridium comprising several layers of compressed cells, brown at the base, dark brown outwardly. Hamathecium comprising numerous, hypha-like, flexuose, paraphyses. Asci 8-spored, unitunicate, obclavate to cylindrical, with a discoid, J+ subapical ring. Ascospores overlapping uniseriate or biseriate, obovoid to ellipsoid, transversally septate, with or Fig. 9 Clypeosphaeria mamillana (holotype). a Herbarium packet. b Herbarium specimens. c Appearance of ascomata on host substrate. d Cross section of ascoma. e Peridium. f, g J+ apical ring of ascus in Melzer’s reagent. h–k Asci. l–o Ascospores. Scale bars: c=500 μm, d= 50 μm, e=10 μm, f, g=5 μm, h–k=10 μm, l–o=5 μm without vertical septa, hyaline. Asexual morph: Coelomycetous. Conidiomata stromatic, scattered to gregarious, subepidermal or subepidermal in origin, immersed to erumpent. Peridium composed of 3–4 layers of cells of textura angularis, outer 1–2-layers brown and inner 1–2 layers hyaline or pale brown. Conidiophores arising from the inner layer cells of basal stroma, absent or reduced to conidiogenous cells, when present long, septate and branched. Conidiogenous cells holoblastic, annelidic, cylindrical or lageniform, hyaline. Conidia cylindrical to clavate, or fusiform, straight or curved, 2–4-euseptate, hyaline, pale olivaceous, or brown, bearing cellular, filiform or attenuated appendages. Type: Discosia Lib., Pl. crypt. Arduenna, fasc. (Liège) 4: no. 346 (1837) Notes: Phylogenetic analyses of combined LSU and ITS sequence data of Discosia and related genera show Discosiaceae taxa to be distinct from the family Bartaliniaceae and Pestalotiopsidaceae. Hence the new family Discosiaceae is introduced for Discosia and Adisciso, Discostroma, Immersidiscosia, Sarcostroma, and Seimatosporium (Fig. 1). Members of the family Discosiaceae are saprobes, endophytes, or foliar pathogens in tropical and temperate regions (Nag Raj 1993; Tanaka et al. 2011). The family presently includes four coelomycetous asexual genera Discosia, Immersidiscosia, Sarcostroma and Seimatosporium, and two sexual genera Adisciso and Discostroma; which were earlier classified under Amphisphaeriaceae. Von Arx (1981) treated Bartalinia, Bartaliniopsis, Bleptosporium, Doliomyces and Hyalotia as synonyms of Seimatosporium. However, Nag Raj (1993) did not accept von Arx’s (1981) treatment and sequence data also does not support this. Discosia Lib., Pl. crypt. Arduenna, fasc. (Liège) 4: no. 346 (1837) Discosia was described by Libert (1837) with D. artocreas (Tode) Fr. as the type. The coelomycetous Discosia is widespread in subarctic, temperate and tropical regions. Discosia species are generally saprobes or plant pathogens and rarely biotrophs (Crous et al. 2013). Discosia neofraxinea W.J. Li, Camporesi & K.D. Hyde, sp. nov. Index Fungorum number: IF551176; Facesoffungi number: FoF 00664; Fig. 10 Etymology: Named because its similarity with Discosia fraxinea. Fungal Diversity Fungal Diversity Fungal Diversity Discosia neofagicola (holotype). a Herbarium specimen. b, c Black conidiomata on the host. d Vertical section of conidioma. e, g Section of peridium. h Germinating spore. f, i–l Conidiogenous cells and developing conidia. m–o Conidia. Scale bars: b= 200 μm, c= 20 μm, d=100 μm, e, g=50 μm, h–j=10 μm, f, i–l=5 μm, m–n=5 μm Fig. 10 Holotype: MFLU 15–0375 Saprobic on dead leaf of Fagus sylvatica. Sexual morph: Undetermined. Asexual morph: Coelomycetous. Conidiomata 125–176 μm high, 450–550 μm diam., stromatic, pycnidioid, solitary or gregarious, superficial, globose to subglobse, unilocular, rugose, dull black, with a short beak, ostiolate. Ostiole 60–80μm diam., circular or oval, opening to the exterior. Peridium 10–20μm wide, composed of two types of pseudoparenchymatous cells, forming thickwalled cells of textura epidermoidea towards the apex, with textura prismatica at the base, with inner layers hyaline gradually merging with the outer dark brown layers. Conidiophores arising from the inner layer cells of the basal stroma, septate, unbranched or occasionally branched, long, cylindrical, hyaline, smooth. Conidiogenous cells 6–40μm long × 1–2 μm wide, holoblastic, cylindrical, hyaline, smooth-walled. Conidia 15–18×2.5–3.5μm (x =16×3μm; n=30), median cells pale brown, naviculate to subcylindrical, narrow towards the base, 3-septate, slightly curved, with cells of unequal length, hyaline to pale brown, bearing appendages; basal cell narrowly obconic with a truncate base bearing a prominent dehiscence scar, with septa thicker and darker than the periclinal wall, with the second cell close to apical cell twice or more than two times that of the size of the third cell close to basal cell, with apical cell subconical and with an acute apex; appendage 10–20μm long, single, unbranched, flexuous. Materials examined: ITALY. Province of Forlì-Cesena [FC], Santa Sofia, Campigna, on dead leaf of Fagus sylvatica, 24 June 2012, Erio Camporesi, IT 469 (MFLU 15–0375, holotype); ex-type living culture, MFLUCC 13–0204, ICMP; ibid. (HKAS 88184, isotype); ibid. Arezzo [AR], Santa Sofia, near Passo della Braccina, on dead leaf of Fagus sylvatica, 30 April 2013, Erio Camporesi, IT 469E (MFLU 15–0736, paratype); living culture, MFLUCC 13– 0378; paratype); ibid. IT 469 F (MFLU 15–0737, paratype). Notes: Discosia neofraxinea is similar to D. artocreas (Tode) Fr. in having 3-septate, hyaline to pale brown conidia with single appendages at each end, but differs in the form of conidiomata and conidiogenous cells. Discosia neofraxinea closely resembles D. fraxinea (Schwein.) Nag Raj which is known from Fraxinus americana. Both share similarities in the dimensions of conidia and conidiogenous cells, but differ in the form of conidiomata (Nag Raj 1993). Discosia neofraxinea has superficial, globose to subglobse, unilocular, rugose, conidiomata, which are much smaller than the applanate to discoid, erumpent, unilocular to bilocular, glabrous, occasionally rugose conidiomata in D. fraxinea (700–1000μm diam., up to 80μm high). Phylogenetically, the strains of D. neofraxinea formed a discreet branch in Discosia. Based on a combination of morphology and phylogeny, we introduce D. neofraxinea as a new taxon. Discosia pseudopleurochaeta Wijayawardene, Camporesi & K.D. Hyde, sp. nov. Index Fungorum number: IF551179; Facesoffungi number: FoF 00661; Fig. 11 Etymology: Named after its morphological similarity with Discosia pleurochaeta Holotype: MFLU 15–0743 Saprobic on dead leaf of Crataegus sp. Sexual morph: Undetermined. Asexual morph: Coelomycetous. Conidiomata 300–360μm diam., 40–80μm high, stromatic, gregarious, occasionally confluent, flattened, glabrous, black, superficial, unilocular. Ostiole papillate, single circular, central. Conidiomata wall thick at the base, 15–25μm, composed of pale brown, thin-walled cells of textura angularis, wall near ostiole thinner, composed of dark brown, thick-walled cells of textura angularis. Conidiogenous cells 3–5×1–3μm, holoblastic, discrete, determinate, conical, hyaline to pale brown, smooth, only in basal conidiomatal wall. Conidia 20–28×3– 5μm (x =23.6×4.11μm, n=20), median cells pale brown, apical and basal cells sub-hyaline to hyaline, subcylindrical, tapered to a truncate base, apex obtuse, straight or slightly curved, dorsiventral, with 3-transverse septate, smoothwalled, bearing appendages at both ends; apical appendage 15–18μm, long, tubular, unbranched; basal appendage 17– 22μm, long, tubular, unbranched. Materials examined: ITALY, Province of Forlì-Cesena [FC], Collinaccia - Castrocaro e Terra del Sol, dead and hanging leaf of Crataegus sp, 20 October 2012, Erio Camporesi, IT 836 (MFLU 15–0743, holotype); ibid (HGUP530 paratype) Notes: Farr and Rossman (2015) list Discosia artocreas (Tode) Fr. and Discosia faginea Lib. from Crataegus spp. Nag Raj (1993) listed the latter species under ‘excluded or unexamined taxa’ as its type species was not available. Discosia artocreas has smaller conidia (conidial dimensions 14–22×2–3μm) than our collection and its appendages are inserted about 2–3 μm from the apical and basal septum (Nag Raj 1993), but in our collection both apendages are inserted very close (1–2μm) to the apical and basal septa. Further we checked Nag Raj (1993) to find other species which have close morphological characters with our collection. Discosia pleurochaeta Mont. & Durieu (19.5–27×3– 3.5μm) has similar conidial dimensions, thus resembling our collection, but has narrower conidia. Further, D. pleurochaeta has longer conidiogenous cells (7–11×2.5–3μm) than our collection. Hence, we introduce a new species to accommodate our new collection based on morphology. Seimatosporium Corda, Deutschl. Fl., 3 Abt. (Pilze Deutschl.) 3 (13): 79 (1833) Fungal Diversity Facesoffungi number: FoF 00842 Saprobic on leaves, stem and bark of various plants, pathogenic on leaves of Eucalyptus spp. Sexual morph: Discostroma-like. Asexual morph: Conidiomata acervular, solitary to gregarious, superficial to immersed, unilocular, globose to subglobose, dark brown to black. Conidiomata wall composed of brown thin or thick-walled textura angularis. Conidiophores cylindrical, filiform, septate, branched, hyaline. Conidiogenous cells holoblastic, annellidic, integrated or discrete, hyaline, determinate. Conidia variable, cylindrical, fusiform or clavate or obovoid, (2 −)3(−5)-septate, aguttulate, medium cells brown, basal cell hyaline, with or without basal appendage, or with the apical cell provided with a single, cellular, simple or branched appendage, basal cell truncate, continuous or occasionally constricted at the septa. Fig. 12 Discostroma fuscellum (MFLU 15–0750). a–c Ascomata on host surface. d, e Section of ascoma. f Peridium. g Paraphyses. h–j Immature asci. k Mature ascus l Mature ascus in Congo red m–o Immature ascospores. p–q Mature ascospores. r Ring-like apical apparatus of ascus in Congo red s Germinating ascospore. t, u Culture on MEA. Scale bars: a–b, h–l=200 μm, c–e=100 μm, f–g, r=50 μm, m– q=20 μm, s=5 μm, t–u=30 mm Notes: Corda (1833) introduced Seimatosporium based on S. rosae as the type species. Sutton (1963), Shoemaker and Muller (1964) and Nag Raj (1993) provided taxonomic notes on the genus. Von Arx (1981) treated Bartalinia, Bartiloniopsis, Bleptosporium, Doliomyces and Hyalotia as generic synonyms of Seimatosporium. Nag Raj (1993) however, accepted Seimatosporium as a valid genus. Nag Raj (1993) mentioned Seimatosporium has Discostroma sexual morphs. Okane et al. (1996) and Fig. 11 Discosia pseudopleurochaeta (holotype). a Conidiomata on leaf. b, c Cross section of stromata. d Cross section of stromatic wall. e–h Developing conidia attached to conidiogenous cells. i, j Conidia. Scale bars: b, c=50 μm, d=25 μm, e–h=8 μm, i, j=20 μm Fungal Diversity Fungal Diversity Hata key ama an d H ar ada (2 004 ) al s o in t r od uce d Seimatosporium spp. with Discostroma sexual morphs. Barber et al. (2011) and Tanaka et al. (2011) also showed that both Seimatosporium and Discostroma cluster in a monophyletic clade in their molecular data analyses. However, both genera lack molecular data for their type species (Tanaka et al. 2011), thus we prefer to maintain both names until the link is proven by molecular analyses. Discostroma Clem., Gen. fung. (Minneapolis): 50, 173 (1909) Notes: Species of Discostroma and Seimatosporium have been linked by molecular data (Tanaka et al. 2011), however the molecular links have not been proven for the types of either genera. Both names should therefore be retained pending fresh collections of the types and a molecular study. Discostroma fuscellum (Berk. & Broome) Huhndorf [as ‘fuscella’], Bull. Ill. nat. Hist. Surv. 34 (5): 520 (1992) Index Fungorum number: IF 359431; Facesoffungi number: FoF 00697; Fig. 12 Saprobic on dead stem of Rosa canina, forming conspicuous, rounded, brown to black ascomata. Sexual morphs: Ascomata 280–478μm diam., 149–227μm high (x =379× 188 μm, n = 30), solitary or clustered, immersed, semiimmersed or superficial, globose to subglobose, membranous, brown, ostiolate. Peridium 25–44μm wide (x =35μm, n=30), three-layered, outer layer comprising brown, thick-walled cells of textura angularis, central layer comprising light brown to yellow, thin-walled cells of textura angularis, inner layer comprising hyaline, thin-walled cells of textura angularis. Hamathecium comprising 3–6μm wide (x =4μm, n=30), numerous, hypha-like, septate, paraphyses, tapering distally, not embedded in a gelatinous matrix. Asci 103– 127×8–11μm (x =115×10μm, n=30), 8-spored, unitunicate, cylindrical, with pedicel often tapering to a point, apically rounded or truncate, with a refractive, J+, bipartite, apical ring. Ascospores 15–18×6–8μm (x =17×7μm, n=30), uniseriate or overlapping uniseriate, fusiform or ellipsoidal, 0–4-septate, hyaline, smooth-walled, thick-walled. Asexual morph: Undetermined. Culture characters: Colonies growing on PDA, reaching 7 cm diam. after 3 weeks at 20–25 °C, with whitened, dense, aerial mycelium on the surface, reverse similar in colour. Material examined: ITALY. Province of Forlì-Cesena [FC], Poggio Baldo - Predappio Alta, on dead stem of Rosa canina, 3 March 2013, Erio Camporesi, IT 1093 (MFLU 15–0750, MFLU 15–0751, MFLU 15–1134, MFLU 15–1135); living culture, MFLUCC 14–0052, ICMP. Notes: There are 30 species recorded under Discostroma (Index Fungorum, 2015) and Paulus et al. (2006) provided a key to 28 Discostroma species. We consider our species to be Discostroma fuscellum based on morphology. Discostroma fuscellum was stated to have immersed perithecia with short protruding papillae, cylindrical asci, with a J+ apical ring and Fig. 13 Seimatosporium cornii (holotype) a, b Conidiomata on Cornus sp. c, d Cross sections of conidiomata. e Paraphyses. f–k Developing conidia attach to conidiogenous cells. l–n Conidia. Scale bars: c, d=100 μm, e=30 μm, f–n=15 μm hyaline, 3-septate, elliptical, pigmented ascospores (Paulus et al. 2006). In our collection the ascospores were hyaline which agrees with Huhndorf (1992). Eriksson (2009) used Leptosphaeria fuscella for Discostroma fuscellum, however, the morphology and phylogenic analyses, show it to belong to Discostroma and is close to Seimatosporium and Tanaka et al. (2011) proposed to use Discostroma fuscellum for this taxon. Seimatosporium cornii Wijayawardene, Camporesi & K.D. Hyde, sp. nov. Index Fungorum number: IF551180; Facesoffungi number: FOF 00662; Fig. 13 Etymology: Named after the host genus on which the fungus occurs. Holotype: MFLU 15–0742 Saprobic on dead branches of Cornus sp. Sexual morph: Undetermined. Asexual morph: Conidiomata 300–330μm diam., 150–175μm high, acervular, unilocular, subglobose, superficial to subepidermal, solitary, dark brown, apapillate ostiolate. Conidiomata wall multi-layered, outer wall thick, composed of brown cells of textura angularis, inner wall thin, hyaline. Paraphyses 30–60μm, cylindrical, filiform, hyaline, aseptate. Conidiophores 13–30×2–4μm, long, cylindrical, branched, hyaline, smooth-walled. Conidiogenous cells holoblastic, annelledic, simple, integrated, determinate, hyaline. Conidia 21–29×9–11 (x =24.9×9.93, n=20), median cells brown to dark brown, basal and apical cells subhyaline to pale brown, clavate or obovoid, occasionally truncate at base, straight to slightly curved, with 3-transverse septate, dark brown septa, aguttulate, smooth-walled, appendage absent. Culture characteristics: slow growing, reaching 2.5–3 cm in 7 days at 18 °C, on PDA, white from above, grey from below, circular, with uneven margin, not zonate, with thin mycelium, becoming dense after 14 days Materials examined: ITALY, Province of Pesaro-Urbino [PU], Monte Nerone, on branches of Cornus sp., 11 June 2012, Erio Camporesi, IT 426 (MFLU 15–0742, holotype); ibid (HGUP531 paratype); ex-type living culture, MFLUCC 14–0467, GUCC 0058. Notes: Farr and Rossman (2015) reported Seimatosporium lichenicola (Corda) Shoemaker & E. Müll. (Conidial dimension 13–15 × 5.5–6.5 μm fide Sutton 1980) and Seimatosporium salicinum (Corda) Nag Raj (11–17×4–6μm fide Nag Raj 1993) from Cornus spp. However, our collection is morphologically distinct from these two species and other known species in Sutton (1980) and Nag Raj (1993). Hence, we introduce this new species based on morphology and molecular data analyses. Fungal Diversity Fungal Diversity Seimatosporium rhombisporum Senan. Camporesi & K.D. Hyde, sp. nov. Index Fungorum number: IF551195; Facesoffungi number: FoF 00693; Fig. 14 Etymology: Species name refers to the rhomboid conidia. Holotype: MFLU 15–0727 Saprobic on dead branches of Vaccinium myrtillus L. Sexual mor ph: Unde termined. Asexual morph: Conidiomata 65–75μm high, 105–125μm diam., (x =70× 120 μm, n = 20), acervular, uniloculate, subglobose to Fig. 14 Seimatosporium rhombisporum (holotype). a Vaccinium myrtillus stems with conidiomata. b, c Conidiomata. d, e Cross section of the conidiomata. f–j Conidia attached to conidiogenesis cells and conidiophores. k–o Conidia. p Germinating conidia. Scale bars: b= 200 μm, c=500 μm, d=50 μm, e–p=10 μm Fungal Diversity subconical, erumpent to superficial, solitary, dark brown. Peridium multi-layered, comprising brown, cells of textura angularis to textura globosa. Conidiophores 1–1.5μm high, 0.5–1μm wide (x =1.3×0.7μm, n=20), cylindrical, branched, hyaline, smooth-walled. Conidiogenous cells 5.5–12.5μm high, 0.5–1μm diam., (x =7.5×0.7μm, n=20), cylindrical, unbranched, holoblastic, anneledic, simple, integrated, hyaline. Conidia 10.5–12×3–4μm (x =11×3.6μm, n=20), clavate, with brown to dark brown median cells and hyaline to pale brown basal and apical cells, pointed at apex, blunt at base, straight to slightly curved, 3-transverse septate, septa thick, smooth-walled, lacking appendages with germinating hyphae originating from basal hyaline cell during germination. Culture characters: Growing on MEA, reaching 1 cm within 7 days when incubated at 18 °C, flat, circular, white colony with smooth margin. Material examined: ITALY, Province of Forlì-Cesena [FC], Santa Sofia, Monte Falco, on Vaccinium myrtillus (Ericaceae), 7 May 2013, Erio Camporesi IT 1228, (MFLU15–0727, holotype) ibid., (MFLU15–0727bis, paratype); living culture MFLUCC 15–0543, ICMP. Notes: We introduce Seimatosporium rhombisporum sp. nov. which differs from other species in having erumpent, conidiomata with long, immersed conidiogenous cells. Conidiomata are filled with conidiogenous cells and conidia form at the apex. The dark, rhomboid conidia are quite specific and phylogenetic analysis (Fig. 1) indicates the uniqueness of this species. However, Seimatosporium vaccinii (Fuckel) B. Erikss. also has conidia without appendages and was obtained from the same host (Lee et al. 2006). The colour and shape of conidia are, however, different. Based on a megablast search of GenBank, the closest hit using LSU sequence data to our strain are Sarcostroma bisetulatum (Guba) Nag Raj (CBS 122695, EU552155; Identities = 883/897 (98 %), Gaps=8/897 (0 %)), Seimatosporium obtusum (H.J. Swart & M.A. Will.) P.A. Barber & Crous (CPC 12935, JN871215; Identities=876/893 (98 %), Gaps=7/893 (0 %)), and S. eucalypti (McAlpine) H.J. Swart (CPC 158, JN871211; Identities=874/892 (98 %), Gaps=8/892 (0 %)). Sarcostroma was introduced to accommodate species having 2–5-septate conidia with only a basal appendage or without any appendages and Sutton (1980) synonymised Sarcostroma under Seimatosporium. Both Seimatosporium obtusum and Seimatosporium eucalypti (McAlpine) H.J. Swart have long, falcate, fusiform or sigmoid conidia with 3–4-septa, rarely 5septa, and basal appendages. These species were excluded from Seimatosporium and included in Vermisporium based on their morphology and phylogeny (Barber et al. 2011). Therefore we introduce Seimatosporium rhombisporum as a new species considering both morphology and phylogeny. Seimatosporium ficeae Dayarathne, Phookamsak & K.D. Hyde, sp. nov. Index Fungorum number: IF551175; Facesoffungi number: FOF 00633; Fig. 15 Etymology: Name reflects the host Ficus, from which this species was isolated. Holotype: MFLU 15–0703 Saprobic on living leaf of Ficus sp. Sexual morph: Undetermined. Asexual morph: Conidiomata 50–60 μm high, 45–90μm diam., acervuli, globose, submerged, solitary, black. Peridium 10–15μm wide, composed of hyaline to pale brown cells of textura angularis to textura globosa. Conidiophores up to 10 μm long, subcylindrical. Conidiogenous cells 13–22μm long, holoblastic, cylindrical to ampulliform, hyaline, thin-walled, smooth, arising from the basal layers of conidiomata. Conidia 15–21×5.5–7.5μm (x = 18×6.5μm, n=30), fusiform, straight, 5-septate, concentric at the septa, with 4 median cells, brown, terminal cells, hyaline, bearing single 8–20μm long, unbranched, central appendages at both ends. Culture characters: Colonies on PDA slow growing, reaching 25 mm diam. after 2 weeks at 25–30 °C, upper side circular, white to gray, and cottony, lower side circular, white to gray. Material examined: CHINA, Yunnan Province, ShangriLa, on leaf spots of Ficus sp., 30 October 2014, R. Phookamsak SGL 002 (MFLU 15–0703 holotype); ex-type living culture, MFLUCC 15–0519=ICMP. Notes: The main differences between Seimatosporium ficeae and other species in Seimatosporium lie in the morphology of the conidia. The conidia of S. ficeae are 5-septate conidia with four, brown median cells and hyaline terminal cells at both ends. S. consocium (Peck) Shoemaker also posses similar morphological characters to S. ficeae, including 5septate with four brown to light brown median cells. Meanwhile, the conidial septation of S. hysterioides (Fuckel) Brockmann also exhibits a similar pattern with five septa but, abundantly they are 3–4-septate. Both S. consocium and S. hysterioides bear central apical appendages and eccentric basal appendages on conidia whereas S. ficeae comprises of central appendages at both ends. In the phylogenetic analysis (Fig. 1), S. ficeae is related to S. hypericinum (Ces.) B. Sutton which has curved, falcate, 4-celled conidia. However, they are well-separated with a high bootstrap support. Seimatosporium vitis Y.P. Xiao, Camporesi & K.D. Hyde, sp. nov. Index Fungorum number: IF551200; Facesoffungi number: FOF 00698; Fig. 16 Etymology: Name referring to the host genus on which the species occurs Holotype: MFLU 15–0746 Saprobic on dead stems of Vitis vinifera, forming conspicuous, rounded, black conidiomata. Sexual morph: Undetermined. Asexual morph: Coelomycetous. Conidiomata 274–368 μm diam., 112–162 μm high (x = 321×137μm, n=30), acervuli, solitary or gregarious, semi- Fungal Diversity immersed, dark brown to black, elongate to rounded or irregular in outline. Peridium 25–39μm wide (x =32μm, n=30), thin, comprising light brown, thin-walled cells of textura angularis. Conidiophore reduced to conidiogenous cells. Conidiogenous cells 1–2μm diam., 13–36μm long (x =1× 25μm, n=30), holoblastic, cylindrical, hyaline to pale brown, thick-walled, smooth. Conidia 34–40×14–17μm (x =15× 37 μm, n = 60), ellipsoid or fusiform, straight or slightly curved, 3-euseptate, constricted at septate, guttulate, bearing an appendage at the base, apical cell conical with broadly rounded apex, two median cells sub-cylindrical, brown to dark brown, relatively thick-walled than the end cells, basal cell Fig. 16 Seimatosporium vitis (holotype) a, b Conidiomata on host surface. c Vertical section of conidioma. d–f Base of conidioma with conidiogenous cells, conidiophores and developing conidia. g–k Mature conidia. l Germinating conidium. m Culture from above on MEA medium. n Culture from below on MEA medium. Scale bars: a= 2.5 mm, b=200 μm, c–d=100 μm, e=40 μm, f–k=20 μm, l=10 μm, m–n=30 mm obconical, pale brown, bearing appendages. Appendages 4– 8μm long (x =5μm, n=30), central or eccentric, single, unbranched, filiform. Culture characters: growing on PDA, reaching 7 cm diam., after 2 weeks at 20–25 °C, with circular, whitened to brown, Fig. 15 Seimatosporium ficeae (holotype). a Pycnidium on host b, c Conidiomata on host tissue. d Section through conidiomata. e Section through peridium. f Conidiogenous cells. g–j. Conidia. k–l Culture of Seimatosporium sp. on PDA. Scale bars: d–e=100 μm, f=50 μm, g–j=10 μm Fungal Diversity Fungal Diversity dense, aerial mycelium on the surface, reverse similar in colour. Material examined: ITALY. Province of Forlì-Cesena [FC], near Predappio Alta, on dead stem of Vitis vinifera L. (Fabaceae), 3 March 2013, Nello Camporesi, IT1092 (MFLU 15–0746, holotype); IT 1092 (MFLU 15–0747, MFLU 15–0748, 15–0749, paratypes); ex-type living cultures, MFLUCC 14–0052, ICMP, MFLUCC 14–0051. Notes: Seimatosporium vitis is similar to S. pistaciae Crous & Mirab. which has conidia with an obtusely rounded apical cell, with a single apical appendage (Crous et al. 2014b). Seimatosporium vitis however, differs in having 3-septa, a conical, apical cell with broadly rounded apex, brown to dark brown, median cells and a pale brown basal cell bearing an appendage. This is the first Seimatosporium species to be described from Vitis. Pestalotiopsidaceae Maharachch. & K.D. Hyde, fam. nov. Index Fungorum number IF551178; Facesoffungi number: 0666 Saprobic or pathogenic on plants. Sexual morph: Ascomata scattered, solitary or in small group, immersed, apex short erumpent. Ostiole subglobose, papillate ostiolar canal periphysate. Peridium comprising several layers of dark brown to blackish, compressed cells. Hamathecium comprising tapering paraphyses. Asci 8-spored, unitunicate, cylindrical, short to long pedicellate, with a J+ or J- apical ring. Ascospores uniseriate, brown, ovoid to elliptic, straight or inequilateral, 2–3-septate. Asexual morph: Coelomycetous. Conidiomata acervular or pycnidial, subglobose, globose, clavate, solitary or aggregated, dark brown to black, immersed to erumpent, unilocular or irregularly plurilocular. Peridium comprising 2–3 strata of textura angularis, outer layer of pale brown, thick-walled cells, becoming hyaline in the inner layer. Conidiophores indistinct, often reduced to conidiogenous cells, when present peripheral, hyaline, branched or unbranched, cylindrical or lageniform. Conidiogenous cells discrete, holoblastic, annellidic, indeterminate, integrated, cylindrical, hyaline, smooth. Conidia ellipsoid to clavate, or fusiform, straight or curved, 3–4-euseptate, hyaline, pale olivaceous or brown, bearing cellular, filiform or attenuated appendages. Type: Pestalotiopsis Steyaert, Bull. Jard. bot. État Brux. 19: 300 (1949) Notes: The new family Pestalotiopsidaceae, which possesses pestalotiopsis-like asexual morphs is introduced for Ciliochorella, Lepteutypa, Monochaetia, Neopestalotiopsis, Pestalotiopsis, Pseudopestalotiopsis and Seiridium (Fig. 1). Maharachchikumbura et al. (2014) suggested that the monotypic genus Pestalotia (1839) might be a synonym of Seiridium (1816), since both genera have similar morphologies. Members of the Pestalotiopsidaceae are common phytopathogens that cause a variety of diseases in plants or are often isolated as saprobes or endophytes and are widely distributed throughout tropical and temperate regions (Guba 1961; Barr 1975; Nag Raj 1993; Maharachchikumbura et al. 2014). The sexual morph of Pestalotiopsis is Pestalosphaeria (Barr 1975) and Seiridium has a Lepteutypa sexual morph (Nag Raj and Kendrick 1985). Pestalotiopsis Steyaert, Bull. Jard. bot. État Brux. 19: 300 (1949) Notes: Based on the conidial forms, Steyaert (1949) split Pestalotia into three genera, namely Pestalotia, Pestalotiopsis and Truncatella. Pestalotiopsis was introduced for species with 5-celled conidia and P. maculans is regarded as the type species. The conspicuous character of this genus is 5-celled, fusiform conidia, with three-coloured median cells, hyaline end cells, and one or more apical appendages. The sexual morph of Pestalotiopsis is Pestalosphaeria and only 13 species are known as compared to the asexual morph (254 species names). Maharachchikumbura et al. (2011) pointed out that the common Pestalotiopsis name should be applied to both morphs. Neopestalotiopsis Maharachch. et al., Stud. Mycol. 79: 135 (2014) Notes: Maharachchikumbura et al. (2014) resolved genera in the Amphisphaeriaceae based on analysis of LSU sequence data. Besides accepting Pestalotiopsis, they introduced Neopestalotiopsis and Pseudopestalotiopsis. Morphologically Neopestalotiopsis can easily be distinguished from Pseudopestalotiopsis and Pestalotiopsis by its versicolorous median cells. Furthermore, in Neopestalotiopsis, conidiophores are indistinct and often reduced to conidiogenous cells (Maharachchikumbura et al. 2014). Pseudopestalotiopsis Maharachch. et al., Stud. Mycol. 79: 180 (2014) Notes: Pseudopestalotiopsis was segregated from Pestalotiopsis based on the type Pseudopestalotiopsis theae (Sawada) Maharachch. et al. (Maharachchikumbura et al. 2014). They designated an epitype for Pestalotiopsis theae (Sawada) Steyaert from fresh leaves of Camellia sinensis collected in Thailand (Maharachchikumbura et al. 2013). Pseu do pes talo tiop sis c a n be di s t i n g u i s h e d f r o m Neopestalotiopsis and Pestalotiopsis by sequence data and in having generally dark, concolourous, median cells with indistinct conidiophores (Maharachchikumbura et al. 2014). Monochaetia (Sacc.) Allesch., Rabenh. Krypt.-Fl., Edn 2 (Leipzig) 1 (7): 665 (1902) [1903] Notes: The genus Monochaetia was introduced by Allescher (1902), with M. monochaeta (Desm.) Allesch. as its type. No sexual morphs are conclusively known for this genus. Steyaert (1949) did not retain Monochaetia as a distinct genus, and transferred many of the species of Monochaetia into Pestalotiopsis or Truncatella. However, Monochaetia was considered as a distinct genus by Guba (1961) based on its single apical appendage. LSU phylogenetic analyses reveal Fungal Diversity Monochaetia to represent a distinct genus from Pestalotiopsis, Seiridium and Truncatella (Maharachchikumbura et al. 2014). It is essential to incorporate molecular data and more taxon sampling in future analyses as Monochaetia includes 3-, 4-, and 6-celled conidial forms (Maharachchikumbura et al. 2014). Seiridium Nees, Syst. Pilze (Würzburg): 22 (1816) [1816– 17] Notes: Sequence data has revealed Seiridium to represent a distinct genus in the family Amphisphaeriaceae, which is characterised by 6-celled conidia (Jeewon et al. 2003b; Maharachchikumbura et al. 2014) and in the present study it is placed in Pestalotiopsidaceae. Blogiascospora and Lepteutypa have been identified as the sexual morph of Seiridium. However, Seiridium is the oldest and most commonly used name and therefore, should be applied to represent both morphs. Ciliochorella Syd., in Sydow & Mitter, Annls mycol. 33 (1/2): 62 (1935) Notes: The genus Ciliochorella was introduced by Sydow and Mitter (1935), with C. mangiferae Syd. as its type. The sexual morph of the genus has not been reported (Hyde et al. 2011), and the characteristic feature of the genus is eustromatic, pycnidial conidiomata; indistinct conidiophores; enteroblastic or phialidic conidiogenesis and 3-euseptate conidia with pale brown median cells and cellular appendages (Nag Raj 1993). Presently seven species are known in Ciliochorella Tangthirasunun et al. (2015). Phlogicylindriaceae Senan. & K.D. Hyde, fam. nov. Index Fungorum Number: IF551190; Facesoffungi number: FoF 00681. Saprobic on leaves, twigs and branches, presently only known from dicotyledons. Sexual morph: Pseudostroma scattered, solitary, immersed, black, thick around papilla, thinner towards the base. Ascomata forming under pseudostroma, solitary, globose, coriaceous, black, ostiolate, papillate. Papilla short, not prominent. Peridium comprising outer, brown, thick-walled cells of textura angularis and inner, hyaline to light brown, thin-walled cells of textura angularis. Hamathecium comprising relatively wide, septate, hyaline paraphyses. Asci 8-spored, unitunicate, cylindrical, with knob-like pedicel, apically rounded, with J+, discoid subapical ring. Ascospores overlapping uniseriate, hyaline, fusiform, equally 1-septate. Asexual morph: Coelomycetous Conidiomata slimy, erect tufts of hyaline conidial masses, synnematous, indeterminate, turning brown with age. Conidiophores consisting of an intricate network of brown, smooth, branched cells. Conidiogenous cells hyaline, annellidic, smooth, becoming pale brown with age, ampulliform with elongated necks, with percurrent proliferations. Conidia formed apically, hyaline, cylindrical with obtusely rounded apex, aseptate to 1-septate. Notes: The new family Phlogicylindriaceae is introduced to accommodate the genus Phlogicylindrium Crous et al. (Summerell et al. 2006). The type species, Phlogicylindrium eucalypti Crous et al. has no known sexual morph and occurred on lesions of living leaves of Eucalyptus in association with a Mycosphaerella species. Phlogicylindrium is unique in having slimy conidiomata, with erect tufts of hyaline conidial masses, and hyaline, unicellular to 1-septate, cylindrical conidia, with obtusely rounded apices (Summerell et al. 2006). The genus comprises three species (Index Fungorum 2015). During studies on Italian ascomycetes, we collected two species that cluster with Phlogicylindrium. Thus we introduce a second genus to Phlogicylindriaceae for which we know the sexual characters. Type: Phlogicylindrium Crous et al., in Summerell et al., Fungal Diversity 23: 340 (2006) Type species: Phlogicylindrium eucalypti Crous et al., in Summerell et al., Fungal Diversity 23: 340 (2006); Fig. 17. Ciferriascosea Senan., Bhat, Camporesi & K. D. Hyde, gen. nov. Index Fungorum Number: IF551191; Facesoffungi number: FoF 00682. Etymology: in honour of late Dr Raffaele Ciferri, an eminent mycologist from Italy. Saprobic on dead plant matter. Sexual morph: Appearing as scattered, black spots, slightly raised on the host surface. Pseudostromata scattered, solitary, immersed, black. Ascomata scattered, solitary, globose, coriaceous, brown to black, ostiolate, papillate. Ostiolar papilla short, narrow, inner wall lined by hyaline periphyses. Peridium comprising outer, brown, thick-walled, layer of cells of textura angularis and inner, hyaline, thick-walled, layer of cells of textura angularis. Hamathecium comprising filamentous, septate, branched, hyaline paraphyses. Asci 8-spored, unitunicate, cylindrical, short pedicellate, with an indistinct, J+ apical apparatus. Ascospores overlapping uniseriate, hyaline, fusiform with narrowly rounded, ends, median uniseptate, slightly constricted at the septum. Asexual morph: Undetermined. Notes: Ciferriascosea is introduced to accommodate two taxa from Spartium junceum , collected in Italy. Morphologically these two species are almost similar. However they differ in dimension of the ascomata, asci and ascospores dimensions. The most obvious character to differentiate these s p e c i e s a r e t h e a s c o s p o r e s e p t a ; C. rectamurum has straight, thick septa while C. fluctamurum has irregular, thin septa. Here we introduced this genus with C. rectamurum as the type species. However we could not obtain the asexual morph from the culture. Combined gene analysis of LSU and ITS sequences of this study shows the close affinity of Ciferriascosea species to Phlogicylindrium species. Ciferriascosea rectamurum Senan., Bhat, Camporesi & K.D. Hyde, sp. nov. Fungal Diversity Index Fungorum Number: IF551193; Facesoffungi number: FoF 00683; Fig. 18 Etymology: Species name based is on the straight, even septa of the ascospores. Holotype: MFLU 15–0726 Saprobic on dead stem of Spartium junceum L. appearing as clear, scattered, black spots, slightly raised on the host surface. Sexual morph: Pseudostromata scattered, solitary, immersed, black. Ascomata 250–360μm high, 340–400μm diam., (x = 265 × 370 μm, n = 20), scattered, solitary, subglobose, coriaceous, black, ostiolate, papillate. Ostiolar papilla short, not prominent, internally covered by periphyses. Peridium 10–20μm wide, (x =15μm, n=20), comprising 4–6 outer layers of brown, thick-walled, cells of textura angularis and 3–5 inner layers of hyaline, thick-walled cells of textura angularis. Hamathecium comprising 2.5–3.5μm wide (x = 2.4μm, n=20), filamentous, septate, branched, paraphyses. Asci 120–140×10–13μm (x =127×12μm, n=20), 8-spored, unitunicate, cylindrical, pedicellate, apically rounded, apical apparatus J+, not prominent. Ascospores 20–26×6–8μm (x = 23×7μm, n=20), overlapping uniseriate, hyaline, fusiform, upper end pointed, lower end rounded, slightly curved, Fig. 18 Ciferriascosea rectamurum (holotype). a Appearance of taxon on host substrate. b. Cross section of pseudostroma and ascoma. c Peridium. d–f Asci. g Paraphyses. h J+ apical ring of ascus mounted in Melzer’s reagent. i–l Ascospores. m Germinating ascospore. n Upper surface of culture. o Lower surface of culture. Scale bars: a=1 mm, b= 100 μm, c–f, h=20 μm, g, i–m=10 μm equally uniseptate, septum straight, slightly constricted at the septum, smooth-walled. Asexual morph: Undetermined. Culture characters: Cultures growing on MEA reaching 1 cm within 7 days when incubated at 18 °C, circular, slightly raised, smooth, entire margin, white, and woolly, with aerial mycelium. Materials examined: ITALY, Province of Forlì-Cesena, Santa Sofia, Castellaccio di Corniolino, on branches of Spartium junceum L (Fabaceae), 29 December 2012, Erio Camporesi, IT 986 (MFLU 15–0726, holotype), ibid., (MFLU 15–0726Q, MFLU 15–0726R, paratypes); ex-type living cultures MFLUCC 15–0542, ICMP. Ciferriascosea fluctamurum Senan., Bhat, Camporesi & K. D. Hyde, sp.nov. Index Fungorum number: IF551194; Facesoffungi number: FoF 00684; Fig. 19. Fig. 17 Phlogicylindrium eucalypti (CBS H-19749, illustration based on Summerell et al. 2006). a Colony sporulating on MEA. b–d Conidiophores and conidiogenous cells in vitro. e Conidiogenous cells. f Conidia. Scale bars: 10 μm Fungal Diversity Fungal Diversity Etymology: species name based on irregular septa in ascospores. Holotype: MFLU 15–0725 Saprobic on dead stem of Spartium junceum L., appearing as slightly raised, scattered, black spots, on the host surface. Sexual morph: Pseudostromata scattered, solitary, immersed, black, clypeate. Ascomata 140–190μm high, 115–170μm diam., (x =176×125μm, n=20), scattered, solitary, globose, brown, ostiolate, papillate. Ostiolar papilla short, distinct, narrow. Peridium 5–8μm wide (x =6.2μm, n=20), comprising 4–6 outer layers of brown, thick-walled, cells of textura angularis and 3–5 inner layers of hyaline, thick-walled cells of textura angularis. Hamathecium comprising 1.2–2.3μm wide (x =1.8μm, n=20), filamentous, septate, branched, paraphyses. Asci 60–75×6.5–8μm (x =66×7.3μm, n=20), 8spored, unitunicate, cylindrical, apex rounded, base with short pedicel, apical apparatus J+, not prominent. Ascospores 9– 10×3–4μm (x =9.4×3.5μm, n=20), overlapping uniseriate, hyaline, fusiform, with narrowly rounded ends, median uniseptate, with a irregular septum, slightly constricted at the septum. Asexual morph: Undetermined. Culture characters: Cultures growing on MEA reaching 1 cm within 7 days when incubated at 18 °C, circular, slightly raised, adpressed to the substrate, smooth, entire margined, white, with sparse aerial mycelium. Material examined: ITALY, Province of Forlì-Cesena [FC], Santa Sofia, Castellaccio di Corniolino, on branch of Spartium junceum L (Fabaceae), 24 March 2013, Erio Camporesi, IT 1141 (MFLU 15–0725, holotype), (MFLU 15–0725bis, isotype); ex-type living cultures MFLUCC 15–0541, ICMP. Notes: Ciferriascosea fluctamurum occurs on Spartium junceum and differs from Ciferriascosea rectamurum in having smaller asci and ascospores. Xylariales The order Xylariales is well-recognized (Maharachchikumbura et al. 2015) and treated with 11 families in this paper. Xylariales comprises Apiosporaceae, Cainiaceae, Coniocessiaceae, Diatrypaceae, Graphostromataceae, Hyponectriaceae, Iodosphaeriaceae, Lopadostomaceae fam. nov., Pseudomassariaceae fam. nov., Vialaeaceae and Xylariaceae. Apiosporaceae K.D. Hyde et al., in Hyde et al., Sydowia 50 (1): 23 (1998) Facesoffungi number: FoF 00629 Saprobic or pathogenic on leaves, stems and roots of monocotyledons, mostly grasses, or endophytic on plant tissues, lichens, and marine algae, occasionally infecting humans, or isolated from soil. Sexual morph: Pseudostromata visible as raised, linear, blackened areas on the host surface, with neck and upper surface visible through slits in the host tissue, mostly gregarious, fusiform, ellipsoid to irregular, black, cells between ascomata and darkened layer above usually thick, composed of brown cells of textura Fig. 19 Ciferriascosea fluctamurum (holotype). a Ascostromata on substrate. b Cross section. c Peridium. d Paraphyses. e–g Asci. h J+ apical ring of ascus mounted in Melzer’s reagent. i–m Ascospores. n Upper surface of culture. o Lower surface of culture. Scale bars: a= 1 mm, b=50 μm, c–h =10 μm, i–m=5 μm angularis. Ascomata solitary or usually gregarious in linear groups, immersed under pseudostromata, globose to subglobose, membranous, papillate, ostiole with periphyses. Peridium composed of several layers, comprising smallish, brown, reddish brown to hyaline cells of textura angularis. Hamathecium composed of dense, hypha-like, long, broad, septate, branched, paraphyses. Asci 8-spored, unitunicate, broad cylindrical to clavate or subglobose, without an apical apparatus. Ascospores overlapping 1–3-seriate to irregularly arranged, hyaline, apiosporous, with a large, straight or curved, upper cell and smaller lower cell, usually surrounded by a gelatinous sheath. Asexual morph: Coelomycetous or Hyphomycetous, Arthrinium spp. Conidiomata sporodochial, solitary to gregarious, immersed, erumpent from host tissue when mature, irregular, black, carbonaceous, coriaceous. Conidiomata basal stroma composed of several layers of dark brown to hyaline cells of textura angularis, with thick side wall, thin at upper and lower walls. Setae absent, or occasionally present, intermingled among conidiophores. Conidiophore mother cells ampulliform, verrucose wall, producing a single single hyphoid, cylindrical, 1–2-septate, verrucose, flexuous conidiophore. Conidiogenous cells basauxic, cylindrical, with/without verrucose wall. Conidia globose to subglobose, dark brown, smooth-walled or with minute wall ornamentations, with a truncate basal scar. Type: Apiospora Sacc., Atti Soc. Veneto-Trent. Sci. Nat., Padova, Sér. 4: 85 (1875), Notes: Apiosporaceae was introduced by Hyde et al. (1998), and is typified by Apiospora. This monophyletic family is characterized by apiospores, and a basauxic, Arthriniumlike conidiogenesis (Samuels et al. 1981; Hyde et al. 1998; Bahl 2006). Basauxic conidiogenesis is not very common, but is a unique type of ‘conidial development’ (Bhat 2010), seen only in some conidial fungi. Examples include genera such as Arthrinium (Apiosporaceae, Xylariales), Dictyoarthrinium (Pezizomycotina incertae sedis), Cordella (Apiosporaceae, Xylariales), Pteroconium (Apiosporaceae, Xylariales) and Spegazzinia (Pezizomycotina incertae sedis) (Ellis 1971, 1976; Kirk et al. 2008; Wijayawardene et al. 2012). However, these are not phylogenetically closely related (Liu et al. 2015), as molecular data only exists for Arthrinium; sequence data atributed to Spegazzinia in GenBank are probably wrong. In basauxic conidiogenesis, each conidiophore consists of a short ‘conidiophore-mother-cell’ (i.e., basal cell, in most cases ampulliform in shape) and an extensible filament arising from within it, which is conidiogenous. In this ‘conidiogenous filament’, the first conidium is always Fungal Diversity Fungal Diversity terminal and holoblastic. Sometimes, this terminal cell also remains sterile (Bhat 2010). Later formed conidia are all of the blastic-type, but develop laterally and basipetally (onebelow the other). Apiospora was previously placed in the family Amphisphaeriaceae (Müller and von Arx 1962). Barr (1976) transferred Apiospora to Hyponectriaceae and later moved the genus to Lasiosphaeriaceae (Barr 1990; Barr and Cannon 1994; Hawksworth et al. 1995). Hyde et al. (1998) reexamined the type species of Apiospora (A. montagnei Sacc.), described a new taxon A. sinensis and introduced the family Apiosporaceae. Phylogenetic analyses have shown that Apiospora is a distinct group accommodated in the order Xylariales (Huhndorf et al. 2004; Zhang et al. 2006; Jaklitsch and Voglmayr 2012; Dai et al. 2014; Sharma et al. 2014). In Crous and Groenewald (2013), Arthrinium is morphologically and phylogenetically circumscribed, and the sexual genus Apiospora is treated as a synonym, on the basis that Arthrinium is the older and more commonly encountered name, and is more frequently used in literature. Arthrinium subglobosa D.Q. Dai & K.D. Hyde, sp. nov. Index Fungorum number: IF551172; Facesoffungi number: FoF 00630; Fig. 20 Etymology: In reference to the subglobose asci. Holotype: MFLU 15–0384 Saprobic on dead bamboo culms. Sexual morph: Pseudostromata visible as raised, blackened areas on the host surface, with parallel spots and upper black surface visible through slits in the host tissue, 1–3 mm long, 300–450μm wide, 150–300μm high, solitary to gregarious, in linear rows, irregular, black, cells between ascomata and darkened layer above usually 50–200μm thick, composed of brown to pale brown, large, thin-walled cells of textura angularis mixed with the host tissues above. Ascomata 200–350μm diam., 150–200μm high, in groups of 3–5, immersed, subglobose with a flattened base, dark brown, coriaceous, papillate, ostiolate at the centre. Peridium laterally 10–20μm thick, composed of dark brown, thick-walled cells of textura angularis. Hamathecium composed of 3–10μm diam., long, filamentous, septate, unbranched, tapering paraphyses. Asci 75–150 × 27–36 μm (x = 105 × 35.6 μm, n = 20), 8-spored, unitunicate, broadly cylindrical, clavate to subglobose, pedicel indistinct, apically rounded, with a shallow apical chamber. Ascospores 24–28×8.5–12.5μm (x =25.8×9.5μm, n=20), overlapping 1–3-seriate, hyaline, apiosporous, clavate to sub-cylindrical, straight or curved, apical cell large, 17–22× 7–12.5μm, with smaller basal cell, 5–10×5–7μm, occasionally at an acute angle from the larger cell at the constricted septum, smooth-walled, guttulate, surrounded by 5–8μm thick gelatinous sheath. Asexual morph: Undetermined. Culture characters: Ascospores germinating on PDA within 24 h and germ tubes produced from the lower cell. Colonies fast growing on PDA, reaching 6 mm in 2 weeks at 28 °C, circular, with an irregular edge, pale brown from above, Fig. 20 Arthrinium subglobosa (holotype). a Appearance of pseudostromata in linear rows. b, d Section through ascoma also showing pseudostroma. c Asci. e Peridium of ascoma. f Paraphyses. g, h Ascospores. i Germinating ascospore. j, k Culture on PDA, k from below. Scale bars: a=1 mm, b=50 μm, c–e=10 μm, f–i=5 μm brown to dark brown in the centre from below. Mycelium superficial to immersed in the media, with branched, septate, smooth hyphae. Material examined: THAILAND, Chiang Rai, Mae Sae Village, on dead culm of bamboo, 14 May 2011, Dong-Qin Dai DDQ00034 (MFLU 15–0384, holotype), living culture, MFLUCC 11–0397, KIB s44, GZAAC. Notes: Arthrinium subglobosa is characterized by linear pseudostromata and broadly cylindrical, clavate to subglobose asci and ascospores which are occasionally sharply curved. Our new strain can be compared with Apiospora bambusae (Turconi) Sivan. and A. setosa Samuels et al. in having almost similar sized asci and ascospores. However, A. subglobosa is phylogenetically separated from A. bambusae and A. setosa based on combined analysis of multigene sequence data (Fig. 1). Other bambusicolous species of Apiospora do not have subglobose asci and the size of asci is less than 27μm wide (Theissen and Sydow 1915; Hyde et al. 1998; Bahl 2006). In Arthrinium subglobosa asci are more than 27μm wide. Arthrinium hyphopodii D.Q. Dai, D.J. Bhat & K.D. Hyde, sp. nov. Index Fungorum number: IF551173; Facesoffungi number: FoF 00631; Fig. 21 Etymology: In reference to the conidia germ tubes producing hyphopodia Holotype: MFLU 15–0383 Saprobic on dead bamboo culms. Sexual morph: Undetermined. Asexual morph: Coelomycetous. Pseudostromata solitary to gregarious, visible as raised, pustulate, blackened areas on the host surface, somewhat linear, with black conidial masses produced through slits in the host tissue. Conidiomata sporodochial, 300–500μm wide, 400–550μm long, 150–250μm high, solitary to gregarious, immersed in host tissue, erumpent when mature, irregular, black, coriaceous. Conidiomata wall 100–150μm thick, lower wall thin, around 10–20μm wide, composed of one layer of dark brown to brown to hyaline cells of textura angularis. Conidiophore mother cells ampulliform, verrucose, producing a single conidiophore. Conidiophores 5–15×2–3.5μm (x = 9.5×2.6μm, n=20), hyphoid, cylindrical, 1–2-septate, strait to flexuous, verrucose. Conidiogenous cells 4–6×2–3.5μm (x =5.5×2.6μm, n=20), basauxic, cylindrical, discrete, with verrucose wall. Conidia 5–10×4–8μm (x =6.5×5.6μm, n= 20), globose to subglobose, dark brown, smooth-walled, with a truncate basal scar, occasionally with a longitudinal, hyaline, thin, germ-slit. Fungal Diversity Fungal Diversity Fig. 21 Arthrinium hyphopodii (holotype). a Appearance of darkened pseudostromata on bamboo host. b–d Pseudostromata on bamboo host with linear cracking. e–g Sections of conidiomata. h Conidioma wall. i–l Conidiophores and conidiogenous cells. m Dark brown conidia. n, o Germinating conidia with hyphopodia. p, q Cultures on PDA. Scale bars: a=5 cm, b=1 mm, c, d=500 μm, e–h=100 μm, l–p=5 μm, q, r= 25 mm Culture characters: Conidia germinating on PDA within 24 h with germ tubes arising from the germ-slit, often terminating in pale to dark brown, branched, lobed, irregular, thick-walled hyphopodia. Colonies growing fast on PDA, Fungal Diversity reaching 90 mm in 2 weeks at 26 °C, cottony, circular, edge irregular, white from above, brown to dark brown in centre from below. Mycelium superficial to immersed in/on media, with branched, septate, smooth, hyphae. Materials examined: CHINA, Kunming, Kunming Institute of Botany, Chinese Academy of Sciences, on dead culm of Bambusa tuldoides Munro (Poaceae), 7 July 2014, Dong-Qin Dai DDQ00280 (MFLU 15–0383, holotype); living culture, MFLUCC 15–003, CBS; Ibid. (isotype in KUN, HKAS 83868). Notes: Arthrinium was previously known as the asexual morph of Apiospora and has more than 50 species listed in Index Fungorum (2015). In Arthrinium conidiogenesis is basauxic (Ellis 1971; Bhat 2010). This new taxon produces brown hyphopodia at the apex of the germ tubes. Such a character has not been observed in other species of Arthrinium. Based on the blast search using the ITS sequence in NCBI’s GenBank, the closest hits are species of A. pterospermum with poor coverage because of the sequence length. A similar search using the LSU sequence showed as closest hits A. ovatum (KF144950; Identities = 867/885 (98 %), Gaps= 3/885 (0 %)), A. phragmites (KF144956; Identities = 865/885 (98 %), Gaps = 3/885 (0 %)) and A. rasikravindrii (KF144961; Identities=866/887 (98 %), Gaps=5/887 (0 %)). Cainiaceae J.C. Krug, Sydowia 30 (1–6): 123 (1978) [1977] Facesoffungi number: FoF 00687 Saprobic on dead grasses and other monocotyledons, appearing as shiny black dots, slightly effuse from the substrate. Sexual morph: Pseudostromata poorly developed or lacking, or sometimes clypeate, scattered, superficial, domeshaped or slightly effuse, smooth, dark brown to black. Ascomata immersed, solitary or aggregated, globose, subglobose or lenticular, coriaceous, brown, with papillate ostiole. Ostiolar papilla short, internally lined by small, hyaline periphyses. Peridium one or two-layered, if two-layered, outer layer comprising thick-walled, brown cells of textura angularis and inner layer comprising thin-walled hyaline cells of textura angularis. Hamathecium comprising abundant, filamentous, septate, paraphyses, slightly constricted at the septa and longer than asci. Asci 8-spored, unitunicate, cylindrical, broadly cylindrical to clavate, short pedicellate, with a complex, layered, J+, cylindrical or dome-shaped apical ring. Ascospores uniseriate, overlapping uniseriate to biseriate, hyaline when young and very dark brown at maturity, ellipsoidal or sphaerical, unicellular to 1-septate, slightly constricted at the septum, wall ornamented with reticulations or longitudinal striations, longitudinal germ slits or germ pores, surrounded by a hyaline gelatinous sheath. Asexual morph: Coelomycetous. Conidiomata pycnidial, scattered, immersed, globose to subglobose, black. Conidiophores hyaline, denticulate, sympodially proliferating. Conidiogenous cells with 1– 3 phialides, filiform, branched or simple, septate, hyaline. Conidia elongate fusiform, falcate to lunate, unicellular or septate, hyaline, with pointed ends. Type: Cainia Arx & E. Müll., Acta bot. neerl. 4 (1): 111 (1955) Notes: The family Cainiaceae was introduced to accommodate species with a unique ascal apical ring, comprising a series of rings and longitudinal germ slits in the ascospores (Krug 1978). Almost all members in this family are from monocotyledons and mostly grasses. In addition to Cainia, Atrotorquata, Arecophila, Ceriophora, Reticulosphaeria and Ommatomyces have been placed in this family at various times (Kohlmeyer and Kohlmeyer 1993; Hyde et al. 1996). Seynesia has also been included based on phylogenetic analysis (Maharachchikumbura et al. 2015). Sequence data are available in GenBank for Arecophila, Cainia and Seynesia and their placement in Cainiaceae is confirmed based on molecular analysis (Smith et al. 2003; Maharachchikumbura et al. 2015). A new Atrotorquata species was introduced as A. spartii (Liu et al. 2015), however analysis of LSU gene sequence data indicate that the phylogenetic placement of A. spartii is not in Cainiaceae although the morphological characters are similar. However combined LSU and ITS gene analysis (Fig. 1) shows the phylogenetic placement of Atrotorquata in Cainiaceae. Hence we include Atrotorquata in Cainiaceae. Another new genus, Amphibambusa was introduced from bamboo in Liu et al. (2015) and LSU gene analysis and morphology indicates the genus belongs to Cainiaceae. The asexual morph of Cainiaceae observed from a pure culture of Cainia desmazieresii has been reported by Muller and Corbaz (1956) as Rhabdospora-like. However, Krug (1978) was unable to obtain the asexual morph from Cainia desmazieresii in culture (Kang et al. 1999b) and we have also been unsuccessful. Type species: Cainia graminis (Niessl) Arx & E. Müll., Acta bot. neerl. 4 (1): 112 (1955) Cainia Arx & E. Müll., Acta bot. neerl. 4 (1): 111 (1955) Facesoffungi number: FoF 00688 Type species: Cainia graminis (Niessl) Arx & E. Müll., Acta bot. neerl. 4 (1): 112 (1955) Basionym: Delitschia graminis Niessl, Notiz. Pyr.: 48 (1876) Saprobic or pathogenic on Poaceae, forming leaf or stem spots, appearing as black, shiny, raised, scattered dots on host surface. Sexual morph: Pseudoclypeus indistinct, scattered, rarely clustered, superficial, dome-shaped, effuse, dark brown to black, each containing a single, or rarely several ascomata, surface smooth. Ascomata immersed, scattered, solitary or aggregated, globose to subglobose, coriaceous, with papillate ostioles. Ostioles short, internally covered with hyaline, filamentous periphyses. Peridium comprising outer layer of thick-walled brown cells and inner layer comprising thickwalled hyaline cells, both of textura angularis. Fungal Diversity Hamathecium comprising numerous, filamentous, unbranched, septate paraphyses, slightly constricted at the septa, longer than asci. Asci 8-spored, unitunicate, cylindrical to clavate, short pedicellate, apex rounded, with a complex, layered, J+, wedge-shaped to cylindrical, apical ring. Ascospores overlapping uniseriate to biseriate, dark brown, ellipsoidal, with one, median septum, constricted at the septum, wall with longitudinal striations, surrounded by a gelatinous sheath. Asexual morph: Coelomycetous. Conidiomata pycnidia, scattered, immersed, globose to subglobose, black. Conidiophores hyaline, denticulate. Conidiogenous cells with 1–3 phialides, filiform, simple or branched, septate, hyaline. Conidia elongate-fusiform to filiform, falcate to lunate, with holoblastic conidiogenesis, unicellular or septate, hyaline, sometimes with pointed ends (description of asexual morph form Krug 1977 and Kang et al. 1999a, b). Notes: Members of Cainia mainly occur on grass hosts. Cainia was introduced by von Arx and Müller (1955) based on Delitschia graminis Niessl and re-named as Cainia graminis. This genus is characterized by immersed ascomata, cylindrical asci with a characteristic J+ apical apparatus with series of rings, brown, two-celled, ascospores with longitudinal striations, and ascospores surrounded by a mucilaginous sheath. Cainia presently comprises C. desmazieri C. Moreau & E. Müll. and C. graminis (Krug 1978). Cainia deutziae E. Müll. and C. cupula (Ellis) E. Müll. were excluded from the genus and placed in Arecophila as Arecophila deutziae (E. Müll.) You Z. Wang et al. (Wang et al. 2004) and Discostroma as Discostroma cupulum (Ellis) M.E. Barr respectively (Barr 1993). Cainia graminis (Niessl) Arx & E. Müll., Acta bot. neerl. 4 (1): 112 (1955) Facesoffungi number: FoF 00689; Fig. 22. Saprobic on leaves of Lolium sp., appearing as black dots on leaf. Sexual morph: Pseudoclypeus scattered, rarely clustered, superficial, dome-shaped, effuse, dark brown to black, each containing a single, or rarely several ascomata, with smooth surface. Ascomata 330–480μm high, 350–515μm diam., (x =400×450μm, n=20), scattered, immersed, globose to subglobose, coriaceous, short papillate, brown, extending slightly above the surface of the pseudoclypeus, with small ostiole. Papilla short, internally covered with hyaline periphyses. Peridium 23–35μm wide (x =29μm, n=20), comprising outer, thick-walled, dark brown cells of textura angularis and inner, thick-walled, hyaline cells of textura angularis. Hamathecium comprising 2.5–4μm wide (x = 3.5μm, n=20), filamentous, septate, hyaline, paraphyses. Asci 170–185×20–30μm (x =180×23μm, n=20), 8-spored, unitunicate, cylindrical to slightly clavate, short pedicellate, narrowly rounded at the apex, apical apparatus distinct, dome-shaped, horizontally divided into a series of 3–4 rings, ring at the apex J-, basal rings J+. Ascospores 26–30×10– 11μm (x =27.5×10.5μm, n=20), biseriate, hyaline when Fig. 22 Cainia graminis (reference specimen). a Appearance of ascomata on host substrate. b Cross section of ascoma. c Peridium. d Paraphyses. e Complex J+, apical ring in Melzer’s reagent. f–i Asci. j– n Ascospores. o Germinating ascospore, germtubes coming from germslit. Note: apical pad of ascospore showing in k. Scale bars: a=500 μm, b=100 μm, c, j–o=10 μm, d, e=5 μm, f–i=20 μm young, becoming pale yellow-brown, and finally very dark brown at maturity, ellipsoidal, broadly narrow and rounded towards the ends, 1-septate, septum in centre, slightly constricted at the septa, with 8 longitudinal striations superficially resembling ridges and an apical thickening or mucilaginous pad of the wall at each end of the spore, surrounded by a hyaline gelatinous sheath, germination via germ slit. Asexual morph: Undetermined. Culture characters: Colonies growing on MEA, reaching 1 cm within 7 days at 18 °C, fast growing, circular, with smooth flat colonies, centre of colony white, woolly and colony margin with unbranched, hyaline, hyphae, tightly adpressed to the media. Materials examined: ITALY, Province of Forlì-Cesena [FC], Santa Sofia, Corniolo, stem of Lolium temulentum L (Poaceae), 25 September 2013, Erio Camporesi, IT 1462 (MFLU 15–0724, reference specimen designated here); living culture, MFLUCC 15–0540, MFLUCC 14–0987, ICMP; Ibid. (MFLU 15–0724bis, MFLU 15–0724tris, duplicates of reference specimen). Notes: Morphological comparison and a Mega blast search using the LSU sequence indicated the closest hit to our strain is Cainia graminis (CBS 136.62, AF431949; Identities=880/ 885 (99 %), Gaps=1/885 (0 %)). As well as morphological characters of our strain were almost identical to Cainia graminis which was illustrated by Krug (1978). However we do not epitype this collection because the host and locality of type material is not the same, and thus designate it as a reference specimen (sensu Ariyawansa et al. 2014). Cainia anthoxanthis Senan., Camporesi & K.D. Hyde, sp. nov. Index fungorum number: IF551188; Facesoffungi number: FoF 00690; Fig. 23. Etymology: Named after the host genus on which the fungus occurs. Holotype: MFLU 15–0539 Saprobic on leaves of Anthoxanthum odoratum L., appearing as black dots on leaf surface. Sexual morph: Pseudoclypeus scattered, superficial, dome-shaped, thick, occasionally slightly effuse, black, each containing a single ascomata, smooth. Ascomata 270–300μm high, 230–250μm diam., (x =280×235μm, n=20), scattered, immersed, globose to subglobose, coriaceous, brown, extending slightly above the surface of the pseudoclypeus, ostiolate, papillate. Papilla short, internally covered with hyaline, filamentous, periphyses. Peridium 10–20μm wide (x =17μm, n=20) outer Fungal Diversity Fungal Diversity Fig. 23 Cainia anthoxanthis (holotype) a Appearance of pseudoclypeus on the host substrate. b Cross section of ascoma showing black pseudoclypeus around the neck c Peridium. d Paraphyses. e–g Asci in water. h–i Asci in Melzer’s reagent with J+ ring. j Apical J+ ring of ascus. k–o Ascospores. p Ascosporic sheath in Indian ink. q Culture growing on MEA from above. r Culture growing on MEA from below. Note: The ascospore in p is mounted in Indian ink and after adding this chemical the texture of the ascospore changed. Scale bars: a=500 μm, b=100 μm, c– d=10 μm, e–p=50 μm layers comprising of thick-walled, dark brown cells of textura angularis and inner layers, of thin-walled, hyaline cells of textura angularis. Hamathecium comprising 2.5–5.5μm wide (x =3.6μm, n=20), filamentous, septate, hyaline, paraphyses. Asci 170–205×30–40μm (x =180×35μm, n=20), sparse, 8spored, unitunicate, ovoid to clavate, slightly curved, with indistinct short pedicel, narrowly rounded at the apex, apical apparatus 6.5–8.5μm high, 6–9.5μm wide (x =8×7.5μm, n= 10), J+, distinct, dome-shaped, divide in two parts by vertical furrows and horizontally divided into a series of 3–5 rings, ring at the apex of the apparatus J-, basal rings J+. Ascospores 25–35×10–15μm (x =30×12μm, n=20) biseriate, hyaline when young, becoming pale yellowish brown and finally very dark brown at maturity, ellipsoidal, broadly narrow and rounded at the ends, 1-septate, septum median, slightly constricted at the septum, with 8 longitudinal striations, superficially resembling ridges and an apical thickening of the wall at each end of the spore, surrounded by a hyaline, gelatinous sheath. Asexual morph: Undetermined. Culture characters: Cultures on MEA slow growing, reaching 1 cm diam. within 10 days at 18 °C, colonies circular, flat, with diffuse margins. Centre of the colony white, woolly, with clusters of mycelia, margin of the colony with unbranched, hyaline, mycelia. Materials examined: ITALY, Province of Forlì-Cesena [FC], Premilcuore, Valbura, on leaves of Anthoxanthum odoratum L. (Poaceae), 2 February 2013, Erio Camporesi, IT 1040 (MFLU15–0723, holotype); ex-type living culture, Fungal Diversity MFLUCC 15–0539, ICMP. Ibid. (MFLU 15–0575, isotype); Ibid. MFLU15–0723bis, MFLU 15–0575bis, MFLU15– 0723tris, paratypes). Notes: Based on a megablast search of the GenBank nucleotide database, the closest hit using the LSU sequence for our collection (MFLU 15–0723) is Cainia graminis (CBS 136.62, AF452033, Identities=900/930 (98 %), Gaps=0/930 (0 %)). Morphologically Cainia anthoxanthis differs from Cainia graminis in having small ascomata, asci with a biseriate ascospore arrangement, a shorter ascal apical ring with a rounded apex and ascospores with a large sheath. The ascospores of Cainia anthoxanthis are more oval at maturity and apical pad is not obvious. However Cainia graminis having more elongated, fusiform ascospores with prominent apical pad. Based on both morphology and molecular phylogeny, Cainia anthoxanthis is identified as a new species of Cainia. Coniocessiaceae Asgari & Zare, Mycol. Progr. 10 (2): 195 (2011) Facesoffungi number: FoF 00671 Saprobic on grasses, soil and dung. Sexual morph: Ascomata small, less than 200 μm diam., superficial, subglobose to pyriform, glabrous or pilose, ostiolate, commonly with hypha-like ostiolar projections. Peridium thin, membranaceous, translucent or sometimes opaque, outer layer with cells of textura intricata. Hamathecium with abundant or few paraphyses. Asci 4-spored, unitunicate, cylindrical to subcylindrical, J-, without apical ring structures. Ascospores dark brown to black, 1-celled, ellipsoidal or fusiform, smoothwalled, with a distinct germ-slit extending over the entire length of spores. Asexual morph: Hyphomycetous, nodulisporium-like. Conidiophores micronematous to macronematous, simple or branched, smooth-walled or verrucose; hyaline. Conidiogenous cells integrated, terminal, discrete, elongating sympodially, with persistent conspicuous denticles, hyaline. Conidia globose, subglobose to pyriform, smooth-walled or verruculose, with a rounded apex, and hyaline, attenuated and truncated base and a more or less distinct projection at the point of attachment to the conidiogenous cells (Asgari and Zare 2011). Notes: The family Coniocessiaceae was introduced by Asgari and Zare (2011) to accommodate the genus Coniocessia with a nodulisporium-like asexual morph. García et al. (2006) had introduced the genus and placed it in Xylariales genera, incertae sedis. The family is unique in the order Xylariales in having a hyaline asexual morph with polyblastic conidiogenesis (nodulisporium-like), contrasting with the generally pigmented Nodulisporium asexual morphs in Xylariales (Asgari and Zare 2011). Members of Coniocessiaceae can also be distinguished from Xylariaceae in possessing nonstromatic ascomata, and asci with nonamyloid apical structures (Asgari and Zare 2011). Asgari and Zare (2011) introduced four new species in Coniocessia and provided molecular data indicating this was a distinct lineage of Xylariales. Thus they introduced the new family. In this study (Fig. 1) we confirm its distinctness as a family and place it in the order Xylariales. Type: Coniocessia Dania García et al., in García et al., Mycol. Res. 110 (11): 1284 (2006) Type species: Coniocessia nodulisporioides (D. Hawksw.) Dania García et al., in García et al., Mycol. Res. 110 (11): 1285 (2006); Fig. 24. ≡ Coniochaeta nodulisporioides D. Hawksw., Norw. Jl Bot. 25 (1): 15 (1978) Diatrypaceae Nitschke [as ‘Diatrypeae’], Verh. naturh. Ver. preuss. Rheinl. 26: 73 (1869) Facesoffungi number: FoF 00679 Saprobic or pathogenic mostly on trees. Sexual morph: Stromata eustromatic or pseudostromatic, well-developed, immersed to erumpent or rarely superficial, mostly black or dark brown, with somewhat carbonaceous outer layer, inner layer pale, loosely packed, parenchymatous. Ascomata perithecial, immersed in stromatic tissues, globose to subglobose, coriaceous, mostly brown, with long, ostiolar necks. Ostioles sulcate, inner layer covered with hyaline, periphyses. Peridium with two layers, outer layer comprising brown cells of textura angularis and inner layer comprising hyaline cells of textura angularis. Hamathecium comprising long, wide, thin-walled, branched, septate, paraphyses, arising from base of perithecia and slightly constricted at septa. Asci 8-spored or polysporous, unitunicate, cylindrical, with a very long pedicel with a more or less truncate apex, with J-, or J+ apical apparatus. Ascospores hyaline to light brown, crowded, allantoid. Asexual morph: Coelomycetous, libertella-like. Conidiomata on host, acervular, subcortical, astromatic, erumpent, yellow to red, with branched conidiophores, in culture pycnidial, superficial, solitary or aggregated, subconical, globose to subglobose, yellow, dark brown to black, with thick peridium, comprising brown, thick-walled cells of textura angularis with branched conidiophores, arising from pseudoparenchymatous cells or interwoven hyphae. Conidiogenous cells in dense palisades, cylindrical, straight or curved, apically distorted or bearing annellations. Conidia filiform, curved, or rarely straight, with flattened base and blunt apex, hyaline. Type: Diatrype Fr., Summa veg. Scand., Section Post. (Stockholm) 384 (1849) Type species: Diatrype disciformis (Hoffm.) Fr., Summa veg. Scand., Section Post. (Stockholm): 385 (1849) Notes: The family Diatrypaceae was introduced by Nitschke (1869) and is a well-studied family comprising 15 genera (Maharachchikumbura et al. 2015). The type genus is Diatrype, and other genera are Anthostoma, Cryptosphaeria, Cryptovalsa, Diatrypella, Diatrypasimilis, Echinomyces, Eutypa, Eutypella, Leptoperidia, Monosporascus, Pedumispora, Peroneutypa, Phaeoisaria, and Quaternaria (Maharachchikumbura et al. 2015). Sequence data is lacking Fungal Diversity Fungal Diversity Coniocessia nodulisporioides (illustration based on Asgari and Zare (2011) a Masses of ascospores on host surface. b Ascomata. c Hypha-like ostiolar projections. d Paraphyses. e Immature ascus. f Mature ascus. g, h Ascospores. i Conidiophores and conidiogenous cells. j Conidia. Scale bars: a=100 μm, b=50 μm, c–j=10 μm Fig. 24 for Echinomyces, Leptoperidia, Peroneutypa and Quaternaria. Recent papers with sequence data providing backbone trees are Carmaran et al. (2006) and Trouillas et al. (2010) as well as Liu et al. (2015) in which a new species of Diatrype from palms was introduced. In the present account we provide a description and reference specimen (sensu Ariyawansa et al. 2014) for the generic type, Diatrype disciformis (Hoffm.) Fr. and a collection of Eutypa flavovirens (Pers.) Tul. & C. Tul. Diatrype Fr., Summa veg. Scand., Section Post. (Stockholm) 384 (1849) Facesoffungi number: FoF 00702 Saprobic on bark. Sexual morph: Stromata scattered or aggregated on host substrate, sometimes spread over a large area of the host, erumpent to superficial, orbicular, disc-like, with flat or convex surface, sometimes spread over the host evenly, arising through the cracks in bark epidermis or beneath the epidermis, edges of cracks remaining as pointed, angular parts, numerous ascomata immersed in one stroma, ostioles opening to surface appearing as black spots, comprising an outer, dark brown, small, tightly packed, thin parenchymatous cell layer and inner, yellowish white, large, loosely packed, parenchymatous cell layer. Ascomata perithecial, immersed in stromatic tissues aggregated, globose to subglobose, narrowing towards the apex and very narrow at the base of ostiolar canal, pale brown, thin-walled, ostiolate. Ostiolar canal immersed in only dark outer layer of stromata, short, compressed, apex wider than base, periphysate, ostiolar opening covered with carbonaceous, black cells. Periphyses hyaline, filamentous, short, curved towards the cavity. Peridium thin, comprising outer, brown, thick-walled cells of textura angularis and inner, hyaline, thick-walled cells of textura angularis. Hamathecium comprising long, wide, globose to ovoid, thin-walled, branched, septate, paraphyses, slightly constricted at septa, narrowing and tapering towards the blunt apex. Asci 8-spored, unitunicate, long and narrow, with thinwalled pedicel, with cylindrical, thick-walled, swollen upper portion, apex flat, with J-, cylindrical, conspicuous, apical apparatus. Ascospores biseriate, hyaline, allantoid, unicellular, with small, fat globules at each end, thin and smooth-walled. Asexual morph: Coelomycetous, Libertella-like. Conidiomata appearing as brownish yellow, watery, conidial rounded mass on white, mycelial clumps. Pycnidia superficial, solitary or aggregated, subconical, globose to subglobose, shiny, surface smooth, yellow, dark brown to black. Peridium thick, comprising brown, thick-walled cells of textura angularis. Conidiophores branched, arising from pseudoparenchymatous cells or interwoven hyphae. Conidiogenous cells in dense palisades, cylindrical, straight or curved, apically distorted or bearing annellations. Conidia filiform, curved or rarely straight, with flattened base and blunt apex, hyaline. Notes: Diatrype comprises mainly saprobes on decaying wood. A few species are reported as pathogens forming cankers on forest trees. Species in this genus are quite resistant to harsh conditions. The asexual morph of Diatrype is reported as a libertella-like fungus (Adamčíková et al. 2011). Diatrype disciformis (Hoffm.) Fr., Summa veg. Scand., Section Post. (Stockholm): 385 (1849) Facesoffungi number: FoF 00691; Fig. 25. Saprobic on wood with bark. Sexual morph: Stromata 1.5–2 mm wide, scattered on host, erumpent to superficial, orbicular, disc-like, somewhat convex, arising through cracks in bark epidermis, edges of cracks remaining as pointed, angular parts, with numerous perithecia immersed in a single stroma, ostioles opening in the central region of the disc and appearing as black spots, margin of the disk thick, black, composed of an outer, dark brown, small, tightly packed, thin parenchymatous cell layer and inner, yellowish white, large, loosely packed, parenchymatous cell layer. Ascomata 620– 760μm high, 265–360μm diam. (x =345×675μm, n=20), perithecial, immersed in stromatic tissues, aggregated, globose to subglobose, narrowing towards the apex and very narrow at the base of ostiolar canal, pale brown, thin-walled, ostiolate. Ostiolar canal 60–105μm high, 85–140μm wide (x =80×105μm, n=20), immersed in only dark outer layer of stromata, short, compressed, apex wider than base, periphysate, ostiolar opening covered with carbonaceous, black cells. Periphyses hyaline, filamentous, short, curved towards the cavity. Peridium 20–30μm wide (x =25μm, n=20), thin, comprising an outer layer of 5–7 layers of brown, thickwalled cells of textura angularis and a thin, inner strata of 7– 10 layers of hyaline, thick-walled cells of textura angularis. Hamathecium comprising 70–75μm long, 1–2μm wide (x = 72×1.8μm, n=20), globose to ovoid, thin-walled, branched, septate paraphyses, slightly constricted at septa, narrowing and tapering towards the blunt apex. Asci 75–115μm long (x =95μm, n=20), 8-spored, unitunicate, with very long, narrow, thin-walled pedicel, with cylindrical, thick-walled, swollen upper portion, 30–40×5–6μm (x =34×5.4μm, n=20), apex flat, with J-, cylindrical, conspicuous, apical apparatus. Ascospores 5–9×1.5–2μm (x =6.5×1.7μm, n=20), biseriate, hyaline, allantoid, unicellular, thin-walled, with small, fat globules at each end, smooth-walled. Asexual morph: Undetermined. Culture characters: Fast growing, reaching 4 cm within 7 days on MEA, when incubated at 18 °C, circular, with smooth margin, fibrous, with sparse arial mycelium, white. Material examined: ITALY, Province of Forlì-Cesena [FC], Santa Sofia Corniolino, on branch of Ostrya carpinifolia Fungal Diversity Fig. 25 Diatrype disciformis (reference specimen) a Stromata on substrate. b Horizontal section of stroma. c Side view of stroma. d Vertical cross section of stroma. e Cross section of ascomata. f Peridium. g Ostiolar chamber filled with periphyses. h Asci arrangement in hamathecium. i–k Asci. l Paraphyses. m Ascospores. Scale bars: a=1 mm, b-d=500 μm, e, i–l=100 μm, g=50 μm, f, m= 10 μm Scop. (Betulaceae), 14 November 2013, Erio Camporesi, IT 1516 (MFLU 15–0722, reference specimen designated here); living culture, MFLUCC 15–0538, ICMP. Ibid. MFLU 15–0722bis, duplicate of reference specimen). Notes: Diatrype disciformis is the generic type of Diatrype and represents the family Diatrypaceae. During our field survey, we collected D. disciformis associated with a branch of Ostrya carpinifolia from Italy. We obtained herbarium Fungal Diversity specimens of D. disciformis from NY herbarium (NY305625, NY305627 and NY305626) and compared the morphological characters. The morphology of our fresh collection was similar to that of herbarium specimens. However, we could not obtain the holotype specimen. Hence, we designate this as a reference specimen (sensu Ariyawansa et al. 2014). The asexual morph of Diatrype disciformis is reported as Libertellalike, however we did not observe this in culture (Adamčíková et al. 2011). Eutypa Tul. & C. Tul., Select. fung. carpol. (Paris) 2: 52 (1863) Facesoffungi number: FoF 00703 Saprobic on decaying wood and pathogenic on living plants causing dieback and cankers. Sexual morph: Stromata scattered, erumpent to immersed, arising through cracks in bark epidermis, several ascomata immersed in a single stromata, ostioles opening to outer surface of stroma, comprising an outer, black, small, tightly packed, thin parenchymatous cell layer and inner, whitish-yellow to dark yellow, comprising of loosely packed, parenchymatous cells. Ascomata perithecial, immersed in stromatic tissues, aggregated, globose to subglobose, narrowing towards the apex, brown, thin-walled, ostiolate, papillate. Papilla short, erumpent to immersed, conical, apex wider than base, periphysate. Peridium comprising an outer layer of brown, thick-walled cells of textura angularis and inner, hyaline, thick-walled layer of cells of textura angularis. Hamathecium comprising long, wide, septate, hyaline paraphyses, slightly constricted at septa, and narrowing and tapering towards the apex. Asci 8-spored, unitunicate, long, narrow, with thin-walled pedicel, with cylindrical, thick-walled, swollen upper portion, apex flat, with J-, cylindrical, conspicuous apical apparatus. Ascospores biseriate or crowded, hyaline, allantoid, unicellular, thin-walled, with small, fat globules at the ends, smooth-walled. Asexual morph: Coelomycetous. Conidiomata appearing as brownish-yellow, watery, bubblelike, rounded, conidial masses. Pycnidia superficial, solitary or aggregated, subconical, globose to subglobose, shiny, with smooth surface, yellow, dark brown to black. Peridium thick, comprising brown, thick-walled, cells of textura angularis. Conidiophores branched, arising from pseudoparenchymatous cells or interwoven hyphae. Conidiogenous cells cylindrical, in dense palisades, straight or curved, apically distorted or bearing annellations. Conidia hyaline, filiform, curved or rarely straight, with flattened base and blunt apex. Notes: Eutypa comprises pathogens and saprobes and is typified by Eutypa lata (Pers.) Tul. & C. Tul. Eutypa lata is a serious pathogen on grapes forming cankers (Rolshausen et al. 2004). Eutypa species are also very common on decaying wood. However, the relationship between saprobic and pathogenic species is not clarified. Eutypa flavovirens (Pers.) Tul. & C. Tul., Select. fung. carpol. (Paris) 2: 57 (1863) Facesoffungi number: FoF 00692; Figs. 26 and 27. Saprobic on decaying wood with bark. Sexual morph: Stromata 1 –1.5 mm wide, scattered on host, erumpent, arising through the cracks in bark epidermis, cracks angular, with numerous ascomata immersed in a single stromata, ostioles opening to outer surface, appearing as black spots, comprising an outer, dark brown to black, small, tightly packed, thin parenchymatous cell layer and inner, dark yellow, large, comprising of loosely packed, parenchymatous cells. Ascomata 295– 430μm high, 120–210μm diam., (x =330×180μm, n=20), perithecial, immersed in stromatic tissues, aggregated, globose to subglobose, narrowing towards the apex and very narrow at the base of papilla, pale brown, thin-walled, ostiolate, papillate. Papilla 70–90μm wide, 85–120μm high (x =70×105μm, n=20), short, immersed in only dark outer layer of stromata, conical, apex wider than base, periphysate. Peridium 11–16μm wide (x =13μm, n=20), comprising an outer, brown, thick-walled, strata of 6–8 layers of cells of textura angularis and inner, hyaline, thick-walled, strata of 4–6 layers of cells of textura angularis. Hamathecium comprising 3.5–5μm wide (x =4.5μm, n=20), long, septate, paraphyses, slightly constricted at septa, and narrowing and tapering towards the apex. Asci 140–210×9.5–13μm (x =170× 10.5μm, n=20), 8-spored, unitunicate, with narrow, long, thin-walled pedicel, with cylindrical, thick-walled, swollen upper portion, apex flat, with J-, cylindrical, 3.5–8.5μm high, (x =5.6μm, n=20), conspicuous apical apparatus. Ascospores 7–11×1.5–2.5μm (x =8.3×2.1μm, n=20), biseriate, hyaline, allantoid, unicellular, thin-walled, with small, fat globules at ends, smooth-walled. Asexual morph: Mycelial clumps erect, white, floccose on culture. Conidiomata 0.7–1.3 mm diam., appearing as brownish yellow, watery, bubble-like, rounded, conidial masses forming from mycelial clumps. Pycnidia superficial, solitary or aggregated, subconical, globose to subglobose, shiny, with smooth surface, yellow, dark brown to black. Peridium thick, comprising brown, thick-walled, cells of textura angularis. Conidiophores 4.5–5.5μm high, 2–2.5μm wide (x =4.9×2.4μm, n=20) branched, arising from pseudoparenchymatous cells or interwoven hyphae. Conidiogenous cells 9.5–13.5μm high, 2–3μm wide (x = 11.5 × 2.4 μm, n = 20), cylindrical, in dense palisades, straight or curved, apically distorted or bearing annellations. Conidia 30–40 × 1–2 μm (x = 34 × 1.4 μm, n=20), filiform, curved or rarely straight, with flattened base and blunt apex, hyaline. Culture characters: Fast growing reaching 4 cm within 7 days on MEA, when incubated at 25 °C, circular, flat, with diffuse margin, white, and becoming yellowish-white, bright yellow to dull yellow with age. Material examined: THAILAND, Chiang Rai Province, Muang District, near Bandu, Baan Khuakhae, at 31 M. (19° 59’ 52.05” N; 99° 49’ 25.15” E), on decaying twigs, 15 November 2012, K.D. Hyde, CHUNI006 (MFLU15–0741, Fungal Diversity reference specimen designated here); living culture, MFLUCC 13–0625, ICMP. Notes: Fungal taxa in Eutypa are saprobes and some cause serious cankers of forest trees (Trouillas et al. 2011). Eutypa flavovirens was collected from northern Thailand and the Libertella-like asexual morph formed in culture. Eutypa flavovirens is morphologically distinct since it has yellow stromatic tissues. There is no LSU sequence data available for Eutypa flavovirens in GenBank. A BLAST searche of ITS sequence data of this Eutypa flavovirens strain indicated similarity to our Eutypa flavovirens (D13M, AJ302429; Identities=545/572 (95 %), Gaps=12/572 (2 %)). The morphological characters of sexual and asexual morph of Eutypa flavovirens are similar to those of previous studies (Glawe and Rogers 1982; Carmarán et al. 2006; Trouillas et al. 2011) in both morphology and phylogeny. However the specimen collected in Thailand may not be identical to the species which was previously collected in Europe. Graphostromataceae M.E. Barr et al., Mycotaxon 48: 533 (1993) Facesoffungi number: 00624 Saprobic on trunks, branches, and twigs of members belonging to several genera of deciduous trees. Sexual morph: Stromata widely effuse, irregular in outline, with two layered ectostroma and entostroma, ectostroma develops first, young ectostroma surface buff, with matted mycelium of yellow hyphae, ectostroma develops beneath, cinnamon in colour, hard and brittle, composed of dark hyphae intermixed with suberized cells of the periderm, pulvinate in the centre. Ascomata monostichous, immersed in diatrypoid configuration in entostroma, globose, but compressed, bottle–shaped, more rarely pyriform or obpyriform, varying in size with, black, opaque, carbonaceous, doliiform perithecial embedded necks. Peridium comprising hyaline, multi-layered, thin, indistinct, flattened cells, about 10 μm wide. Hamathecium comprising few, sparse, elongate, tapering, thin-walled paraphyses. Asci unitunicate, 8-spored, fusiform to narrowly clavate, rounded to subtruncate at the apex, with J+ apical apparatus. Ascospores 1-celled, hyaline, multiseriately arranged, suballantoid, tapering at both ends, thin-walled. Asexual morph: nodulisporium-like (Barr et al. 1993) Type genus: Graphostroma Piroz., Can. J. Bot. 52 (10): 2131 (1974) Graphostroma Piroz., Can. J. Bot. 52 (10): 2131 (1974) Facesoffungi number: 00704 Saprobic on trunks, branches, and twigs of members of several genera of deciduous trees. Sexual morph: Ectostroma cinnamon in colour in young stromata, hard and brittle, composed of dark hyphae with periderm cells, at maturity ectostromatic crust separates exposing the entostromatic “disc”, with ostioles, dark cinnamon to chestnut brown, pulvinate in the centre, with a thin, sterile, fimbriate margin. Ascomata more or less monostichous, compressed globose, Fig. 26 Diatrype disciformis (reference specimen). a, b Stromata on substrate. c Horizontal section of stroma. d Vertical section of stroma. e Ostiolar chamber with periphyses. f Cross section of ascomata. g Paraphyses. h Peridium. i–m Asci. n Ascospores. o Culture from above. p Culture from below. Scale bars: a, b=500 μm, c=1 mm, d= 200 μm, e=50 μm, f=100 μm, g–n=10 μm bottle-shaped, more rarely pyriform, or obpyriform, varying in size from specimen to specimen. Peridium hyaline, composed of indistinct, tangentially flattened cells. Hamathecium comprising hyaline, very thin-walled, inconspicuous narrowly ventricose paraphyses, 3-septate, tapering gradually to a pointed apex. Asci 8-spored, unitunicate, clavate, subsessile to short-stalked apex rounded to subtruncate, with a minute J+ apical apparatus. Ascospores overlapping biseriate, hyaline, suballantoid, tapered at both ends, inequilateral, thin-walled, with longitudinal germ-slit. Asexual morph: nodulisporiumlike. Conidiogenous cells thin-walled, often roughened at the base, apex swollen into a clavate or globose vesicle, dilute straw coloured towards the base, hyaline above, bearing up to 12 small, inconspicuous denticles, arising singly from the apex or sides of more or less erect mycelial hyphae, or in whorls of three to five from apices of erect hyphae, Conidia pyriform to turbinate, rounded above, hyaline, 1-celled, strongly attenuated towards a minute thickened and refractive basal scar, containing a single, large guttule. Type species: Graphostroma platystoma (Schwein.) Piroz., Can. J. Bot. 52 (10): 2131 (1974) Basionym: Diatrype platystoma (Schwein.) Berk., Grevillea 4 (no. 31): 95 (1876) Facesoffungi number: FoF 00711; Fig. 28. Notes: Pirozynski (1974) observed a North American species resembling Diatrype stigma (Hoffm.) Fr. and placed it in a new monotypic genus Graphostroma, as G. platystoma (Schwein.) Piroz. Pirozynski (1974) observed an association between Graphostroma and a nodulisporium-like sp. in herbarium specimens and in culture which was identical to most of the conidial states of Hypoxylon. Therefore Pirozynski (1974) placed Graphostroma platystoma in Xylariaceae. Barr and Boise (1985) positioned G. platystoma as atypical in Xylariaceae and in Barr et al. (1993) introduced a new monotypic family for Graphostroma based on the combination of its nodulisporium-like conidial state and diatrypaceous sexual morph with allantoid ascospores. In the present phylogenetic analysis Graphostroma platystoma clusters together with the species belongs to Hypoxylaceae (Fig. 1), while in Maharachchikumbura et al. (2015) Graphostromataceae was placed between Xylariaceae and Diatrypaceae. The current, preliminary phylogeny based on rDNA data raises some doubt as to whether the status of the Graphostromataceae, as a separate family, will be supported by molecular data. The species G. platystoma is defined by having a sexual morph similar to that of Diatrypaceae, while Fungal Diversity Fungal Diversity Fig. 27 Asexual morph of Eutypa flavovirens (MFLU 15–0741) a, b Arial clumps formed by mycelium. c, d Conidiomata on MEA. e Cross section of conidioma. f Peridium. g–k Conidia attached to conidiophores. l–o Conidia. Scale bars: a–d=1 mm, e=100 μm, f=50 μm, g–o=10 μm showing the characteristic asexual morph of the hypoxyloid Xylariaceae. In the concept of Barr, which heavily relied on the spore morphology, the family was therefore regarded as intermediate between Diatrypaceae and Xylariaceae. On the Fungal Diversity Fig. 28 Graphostroma platystoma (redrawn from Pirozynski 1974). a Ascomata in cross section. b Ascus and paraphyses from DAOM 127673. c Ascus from DAOM 75729. d Ascospores from DAOM 36086. e Ascospores from DAOM 65174. f Conidia. g Conidiophores and conidia from DAOM 134022. h Conidiophores and conidia from DAOM 36091. Scale bars: b–f=20 μm Fungal Diversity other hand, it has become clear that phylogenetic relationships within the Xylariaceae are corroborated by the type of asexual morph, and the current study clearly revealed a rather distant relationship of Graphostroma with Diatrypaceae. Morphologically similar xylariaceous genera with bipartite stromata such as Biscogniauxia, Camillea and Obolarina will need to be studied in detail to verify this hypothesis, using a multigene genealogy. Hyponectriaceae Petr., Annls mycol. 21 (3/4): 305 (1923) Saprobic on dead plant matter. Sexual morph: Appearing as black, shiny spots on host surface or small black lines arising from cracks in bark. Pseudostromata present or lacking, if present, superficial, forming clypeus over the ascomata. Ascomata solitary or aggregated, immersed, erumpent or rarely somewhat superficial, globose to ovoid, upright or horizontal, brown to black. Papilla short, ostiolate with or without periphyses. Peridium comprising two layers, outer layer of brown, cells of textura angularis, inner layer of hyaline cells of textura angularis, in some genera cells are of textura globosa. Hamathecium of sparse, septate, paraphyses, tapering towards the apex, often deliquescing at maturity. Asci 8-spored, unitunicate, oblong, cylindrical or ellipsoidal, short pedicellate, with a J+ or J- apical apparatus. Ascospores overlapping biseriate, or fasciculate, hyaline, yellow to light brown, fusoid, isthmoid, elongate filiform, obovoid, or oblong, asymmetric or symmetric, unicellular or septate, smoothwalled or verruculose, with or without a mucilaginous sheath. Asexual morph: Undetermined. Type genus: Hyponectria Sacc., Michelia 1 (no. 2): 250 (1878) Notes: Hyponectriaceae was introduced by Petrak (1923) to accommodate both Hyponectria and Anisostomula. Seventeen genera were included into the family Hyponectriaceae by Hawksworth et al. (1995). Most genera were, however, excluded from the family by Hyde et al. (1998), while Palmomyces and Charonectria were added (Hyde et al. 1998; Rossman et al. 1999). In addition to Hyponectria, this family comprises of Apiothyrium, Arecomyces, Arwidssonia, Cesatiella, Chamaeascus, Discosphaerina, Exarmidium, Frondicola, Micronectria, Pellucida, Physalospora, Phragmitensis, Pseudomassaria, Rachidicola and Xenothecium (Wang and Hyde 1999; Sivanesan and Shivas 2002). This is a problematic family and it is not clear if it belongs in Xylariales. The type species Hyponectria buxi (DC.) Sacc. needs recollecting and sequencing as the sequence data in GenBank may be inaccurate (Jaklitsch and Voglmayr 2012). The other genera in this family should also be recollected and sequenced to establish affinities. In this paper we include 17 genera in Hyponectriaceae. Type species: Hyponectria buxi (DC.) Sacc. Hyponectria buxi (DC.) Sacc., Michelia 1 (no. 2): 250 (1878) ≡ Sphaeria buxi DC., in de Candolle & Lamarck, Fl. franç., Edn 3 (Paris) 6: 146 (1815) Fig. 29. Saprobic on leaves of Buxus sp. Ascomata immersed, depressed globose, visible as orange to brown dots on the host surface, coriaceous, ostiolate, solitary or mostly gregarious. Ostiole aperiphysate. Peridium one strata of 2 layers of cells textura angularis with thickened brown walls around ostiole. Paraphyses not observed. Asci 8-spored, unitunicate, cylindric-clavate to clavate, short pedicellate, with an indistinct, J- apical apparatus. Ascospores overlapping biseriate or obliquely uniseriate, hyaline, ellipsoidal or oblong, straight or inequilateral, 1-celled, contents minutely guttulate, lacking a sheath. Material examined: UK, Surrey, Mickleham, on leaves of Buxus sp., 1927, E.W. Mason no. 365 (IMI 16895). Iodosphaeriaceae O. Hilber, in Hilber & Hilber, The Genus Lasiosphaeria and Allied Taxa (Kelheim): 7 (2002) Facesoffungi number: FOF 00849; Fig. 30. Saprobic on host surface. Sexual morph: Ascomata superficial, solitary, black, and easily removed from the substrate, ascomata covered with dark brown, setae-like hairs, comprising agglutinated mycelial strands, with a stellate arrangement, arising from cells at the perithecial surface. Ostiole pore-like, periphysate. Peridium comprising outer angular, pigmented, brown cells, inner cells flattened, hyaline. Hamathecium comprising numerous, hypha-like, septate, flexuose, paraphyses, slightly tapering towards the apex. Asci 8-spored, unitunicate, narrowly clavate, short-pedicellate or apedicellate, apically rounded, with a J+ subapical ring. Ascospores biseriate, allantoid, unicellular, hyaline, smooth-walled, lacking sheaths or appendages. Asexual morph: ceratosporium-like conidia have been observed on the surface of perithecia, but may not be related. Notes: The family Iodosphaeriaceae was introduced by Hilber and Hilber (2002) to accommodate the genus Iodosphaeria. The genu s is unique in the order Amphisphaeriales as it has superficial ascomata covered with dark brown, setae-like hairs, comprising agglutinated mycelial strands, with a stellate arrangement. Li et al. (2015b) introduced a new species, Iodosphaeria tongrenensis Li et al., bringing the total no. of species in the genus to nine. Li et al. (2015b) provided molecular data that showed this taxon clustered in Amphisphaeriaceae sensu stricto and in this study (Fig. 1) we confirm its distinctness as a family. Type: Iodosphaeria Samuels et al., Mycotaxon 28 (2): 486 (1987) Type species: Iodosphaeria phyllophila (Mouton) Samuels et al., Mycotaxon 28 (2): 486 (1987) Material examined: NEW ZEALAND: Kaipara Harbour, Mt Auckland, Atuanui State Forest; Cyathea dealbata, November 1973, K.D. Hyde, GJS73-260 (PDD 32622, holotype). Fungal Diversity Fig. 29 Hyponectria buxi (redraw from plate in Wang and Hyde (1999), IMI 16895) a Ascomata on host substrate. b Section of ascoma. c–e Asci. f–h Ascospores. Scale bars: a–b= 40 μm, c–h=16 μm Notes: When Samuels et al. (1987) introduced the genus Iodosphaeria they deposited a specimen collected in New Zealand in PDD and this was used in this study to illustrate the family. Lopadostomaceae Daranagama & K.D. Hyde, fam. nov. Index fungorum number: IF551174; Facesoffungi number: FoF 00071 Saprobic on dead wood. Sexual morph: Pseudostromata (for Lopadostoma) immersed to erumpent, visible as darkened areas, densely gregarious, scattered, compact. Stromata (for Creosphaeria) erumpent to nearly superficial, with abrupt margins, containing one to few perithecia, frequently coalescent in linear rows, dull black, waxy, roughened, flattened at the top, inconspicuous perithecial mounds. Ascomata multiperithecial, single to multi-layered, arranged in a cluster, with long ostiolar necks (for Lopadostoma), with ostiolar canal lined with whitish material (for Creosphaeria). Ostioles umbilicate or at the same level as stromatal surface. Peridium amorphous, comprising dark brown to black cells. Asci (4–)8-spored, cylindrical, pedicellate, apically rounded, with a J+ apical apparatus. Ascospores uniseriate or partly biseriate, oblong, at first hyaline, turning light brown to nearly black, narrowly ellipsoid, smooth-walled, with full length germ slit. Asexual morph: Libertella-like. Conidiomata convoluted or multi-loculate, with black pycnidial wall. Conidiophores dior trichotomously branched, stipes yellow, above hyaline, smooth-walled, arranged in dense palisade. Conidiogenous cells terminal, cylindrical, smooth-walled, bearing conidial secession scars. Conidia hyaline, falcate, strongly curved to semicircular, unicellular. Type: Lopadostoma (Nitschke) Traverso, Fl. ital. crypt., Pars 1: Fungi. Pyrenomycetae. Xylariaceae, Valsaceae, Ceratostomataceae 1 (2): 169 (1906) Basionym: Anthostoma subgen. Lopadostoma Nitschke, Pyrenomyc. Germ. 1: 121 (1867) Notes: One of the hallmarks of most xylariaceous taxa are the hyphomycetous asexual morphs. There are, however, certain exceptions where taxa produce coelomycetous asexual morphs that have affinities with other families. In our phylogenetic analysis Lopadostoma was placed outside Xylariaceae with Creosphaeria sassafras (Schwein.) Y.M. Ju et al. and appears as a separate monophyletic group. The same placement was also observed by Jaklitsch et al. (2014). Lopadostoma and Creosphaeria are not very similar in morphology and Ju et al. (1993) believe they should represent two different genera. Jaklitsch et al. (2014) revised the genus Fungal Diversity Lopadostoma and also reported a Libertella asexual morph. Creosphaeria was introduced by Theissen (1910) with having soft, light coloured stromata. Petrini and Müller (1986) reported a Libertella asexual morph from culture. Ju et al. (1993) observed the same type of conidia in the sexual morph. Lopadostoma and Creosphaeria form separate monophyletic groups, in a distinct, but previously unrecognized evolutionary line. Thus we introduce a new family, Lopadostomaceae, in the order Xylariales, to accommodate Lopadostoma and Creosphaeria. Creosphaeria may later require its own family following more collections. Lopadostoma (Nitschke) Traverso, Fl. ital. crypt., Pars 1: F u n g i . P y r e n o m y c e t a e . X y l a r i a c e a e , Va l s a c e a e , Ceratostomataceae 1 (2): 169 (1906) Facesoffungi number: FoF 00706 Saprobic on outer surface of wood and branches. Sexual morph: Pseudostromata subglobose to conical, pustulate or widely effuse, immersed, erumpent from bark, surrounded by a narrow, black, carbonized encasement visible as “black line”, containing one to many groups of perithecia, ectostromatic disc visible, surrounded by blackened bark surface visible as a darkened area. Ectostromatic disc discrete to fused, convex or pulvinate, flattened or raised, dark brown, grey or black, smooth or tubercular. Ostiole inconspicuous and umbilicate, rarely convex, shiny black, or distinctly projecting and cylindrical. Ascomata clustered in valsoid groups, single to double layers or rarely multi-layers, subglobose or flask-shaped. Peridium amorphous, comprising dark brown to black cells, with convergent ostiolar necks. Hamathecium comprising numerous, long, apically free, rarely branched paraphyses. Asci 8-spored, unitunicate, cylindrical, pedicellate, with a globose to ellipsoid, J+ apical apparatus, containing a lower flat ring. Ascospores unicellular, oblong to narrowly ellipsoid, at first hyaline, becoming pale brown and finally dark to blackish brown at maturity, smooth-walled, germ slit straight through entire spore length, with 2 large guttules when immature and a narrow hyaline epispore. Asexual morph: Coelomycetous, Conidiophores libertella-like, hyaline, rarely branched. Conidia unicellular, hyaline, falcate, with acute upper end and narrowly truncate lower end, curvature varying according to the species (Jaklitsch et al. 2014). Type species: Lopadostoma turgidum (Pers.) Traverso, Fl. ital. crypt., Pars 1: Fungi. Pyrenomycetae. Xylariaceae, Valsaceae, Ceratostomataceae 1 (2): 170 (1906) Basionym: Sphaeria turgida Pers., Observ. mycol. (Lipsiae) 1: 17 (1796) Facesoffungi number: FoF00708; Fig. 31. Notes: Lopadostoma turgidum was lectotypified by Lu and Hyde (2000), which turned out to be invalid because the selected material was not from the original collection. Hence, Jaklitsch et al. (2014) observed Persoon’s original material (which is impossible to observe without actually going to L) Fig. 30 Iodosphaeria phyllophila (holotype) a Herbarium packet. b Herbarium material. c, d Ascomata on the host substrate. e Section of ascoma. f Peridium. g Ceratosporium conidia seen from the surface of perithecia. h–i J+ subapical ascal ring in Melzer’s reagent. j–m Asci (m stained in Melzer’s reagent). n–q Ascospores. Scale bars: c=1 mm, d= 500 μm, e=50 μm, f=10 μm, g=20 μm, h–i=5 μm, j–m=10 μm, n–q= 5 μm and epitypified Lopadostoma turgidum with a fresh collection. Material examined: AUSTRIA, Niederösterreich, Gaaden, 3 December 2011, H. Voglmayr (WU 32085, epitype). Melogrammataceae G. Winter [as ‘Melogrameae’], Rabenh. Krypt.-Fl., Edn 2 (Leipzig) 1.2: 797 (1886) Facesoffungi number: FoF 00840 Saprobic on woody plants. Sexual morph: Stromata solitary, scattered or aggregated in lines, superficial, erumpent from bark, pulvinate or discoid, soft textured, internally comprising reddish-brown, pseudoparenchymatous cells, apex papillate with black ostiole. Ascomata perithecial, sometimes confluent, immersed, globose, medium, dark brown to black, ostiole periphysate. Peridium comprising rows of cells, brown externally, hyaline internally. Hamathecium of septate, hyaline, paraphyses. Asci 8-spored, unitunicate, clavate or fusoid, straight, curved or sigmoid, short pedicellate, with shallow, nonamyliod, apical ring. Ascospores hyaline or brown, filiform, straight or cylindrical or falcate, 0–3-septate, end cells slightly entirely lighter or only at their tips, end narrowly rounded to subacute, with a smooth narrow hyaline perispore, often with one large guttule in each cell, wall smooth. Asexual morph: Coelomycetous. Conidiophores long, cylindrical, stiffly upright, septate, verticillate and whorled or not. Conidiogenous cells holoblastic, proliferating sympodially, hyaline, thin. Conidia elongate, falcate. Type: Melogramma Fr., Summa veg. Scand., Section Post. (Stockholm): 386 (1849) Type species: Melogramma campylosporum Fr., Summa veg. Scand., Section Post. (Stockholm): 386 (1849) Notes: Melogramma was introduced by Fries (1849), with Melogramma campylosporum as the type species. The family Melogrammataceae was introduced by Winter (1884–1886) to accommodate Botryosphaeria, Endiothia, Valsaria and Melogramma. This family is characterized by pulvinate or discoid stromata, with papillate black ostiolar dots, fusoid, falcate, curved or sigmoid asci, and often strongly curved ascospores (Jaklitsch and Voglmayr 2012). Melogramma campylosporum Fr., Summa veg. Scand., Section Post. (Stockholm): 386 (1849) Facesoffungi number: FoF 00841, Fig. 32 Basionym: Sphaeria melogramma (Bull.) Pers., Syn.meth. fung. (Göttingen) 1:13 (1801). Possible synonyms (see Index Fungorum 2015) Saprobic on dead shoots of Carpinus betulus L. Sexual morph: Stromata 545–694μm high, 1047–1668μm diam., Fungal Diversity Fungal Diversity solitary, scattered or aggregated in lines, superficial, erumpent from bark, pulvinate or discoid, soft textured, reddish-brown, internally comprising pseudoparenchymatous cells, apex papillate with black ostioles. Ascomata perithecial, (138–) 163– 287 (−297) μm high×(73–) 135–278 (−284) μm diam. (x = 225×206μm, n=10), sometimes confluent, immersed, globose, dark brown to black, ostiolate. Ostiole 54–72μm diam., with periphyses. Peridium 16–42μm wide, comprising two layers, outer layer, composed of dark brown to dark cells of textura angularis to prismatica, inner layer hyaline, with thin– walled cells. Hamathecium of 3–4μm diam., septate, paraphyses. Asci (82–) 93–119 (−132)×(10–) 11–14 (−15) μm (x = 106×13μm, n=30), 8-spored, unitunicate, clavate or fusoid, straight, curved or sigmoid, short pedicellate, with shallow, Japical ring. Ascospores (23.5–) 33–42 (−46)×(3.5–) 4–5 (−6) (x =37.6×4.7μm, n=50), young ascospores hyaline, straight and 0–1-septate, mature ascospores brown, falcate, often strongly curved, 3-septate, end cells slightly entirely lighter or only at their tips, ends narrowly rounded to subacute, with a smooth, narrow, hyaline perispore; often with one large guttule in each cell, smooth-walled. Material examined: AUSTRIA, Styria, Graz, distr. Mariatrost, on the north-facing hillside below the church, clearing, 47°06’29”N, 15°29’32”E, MTB 8858/4, c. 440 m alt.; 5 November 2006, C. Scheuer #5321 (S, F123341). Pseudomassariaceae Senan. & K.D. Hyde, fam. nov. Index fungorum number: IF551208; Facesoffungi number: FoF 00843 Saprobic on recently dead twigs attached to the trees, appearing as black dots, below small raised areas of bark, opening through long fissures. Sexual morph: Ascomata perithecial, scattered, solitary or aggregated, immersed, depressed globose to ellipsoid, coriaceous, black, ostiolate, papillate. Ostiolar papilla short, cylindrical, wide at the apex, periphysate. Peridium comprising strongly compressed, narrow, light to medium brown, thin-walled cells of textura angularis. Hamathecium comprising numerous, apically narrow, basally wide, hyaline paraphyses. Asci 8-spored, unitunicate, clavate to fusoid, short pedicellate, apically rounded, with J+, or J– apical ring. Ascospores biseriate or partially uniseriate, hyaline, broadly ellipsoid, oblong or narrowly clavate, mostly apiosporous, with a rounded to subconical, small, lower cell or equally uniseptate, straight or curved, thick-walled, smooth-walled. Asexual morph: Hyphomycetous, Setae erect, dark brown, straight to flexuous, arising from superficial hyphae, branched at base, subcylindrical, tapering to obtuse apex, 3–7-septate, basal cell slightly swollen. Conidiophores subcylindrical to setiform, with radially lobed basal cells, pale brown, smooth, arising from superficial mycelium, straight to flexuous, 1–10-septate. Conidiogenous cells terminal or lateral, polyblastic, subcylindrical to somewhat clavate, pale brown, smooth, with 1–4 denticulate loci. Conidia lageniform, distal end free, Fig. 31 Lopadostoma turgidum (epitype). a Herbarium specimen. b Pseudostromata in face view. c Ectostromatic disc. d, e Transverse pseudostroma sections. f, g Vertical pseudostroma sections. h Apical ring bluing in Melzer’s reagent. i Peridium. j Paraphyses. k–o Asci. p,r,s Ascospores showing germ slit. q Ascospore. Scale bars: b = 500 μm, c–e=200 μm, f–g=100 μm, h, j=10 μm, I=20 μm, k–o= 50 μm, p–s=5 μm truncate, rostrate at proximal end, pale brown, with a subhyaline transverse band at equatorial zone, smooth, guttulate, aseptate. Notes: Pseudomassaria was introduced by Jaczewski (1894) to accommodate Sphaeria chondrospora. This genus has been synonymised under Aplacodina, Apiospora and Apiosporella (Barr 1964). Hyde et al. (1998) however, assigned this genus to family Hyponectriaceae introducing a new species as Pseudomassaria huwerae. Lumbsch and Huhndorf (2010) also listed Pseudomassaria under Hyponectriaceae as did Maharachchikumbura et al. (2015). Combined gene analysis herein (Fig. 1), show the taxonomic placement of Pseudomassaria different from Hyponectriaceae. This result confirms the taxonomic placement suggested by Jaklitsch and Voglmayr (2012). The asexual morph of Pseudomassaria carolinensis M.E. Barr & Hodges was reported as Beltraniella portoricensis (F. Stevens) Piroz. & S.D. Patil) (Hodges and Barr 1971). All species of Pseudomassaria species do not have similar morphological characters and phylogenetically they do not cluster together. Leiosphaerella also shows molecular and morphological similarities with Pseudomassaria (Jaklitsch and Voglmayr 2012). Single gene sequence analyses of ITS and LSU regions in Jaklitsch and Voglmayr (2012) indicate a separate taxonomic grouping for the Pseudomassaria, Leiosphaerella clade. Therefore in this study we introduce a new family Pseudomassariaceae to accommodate Leiosphaerella and Pseudomassaria. Type: Pseudomassaria Jacz., Bull. Herb. Boissier 2: 663 (1894) Type species: Pseudomassaria chondrospora (Ces.) Jacz., Bull. Herb. Boissier 2: 663 (1894) ≡ Sphaeria chondrospora Ces., Hedwigia 1: tab. 11, Fig. 2 (1855) Pseudomassaria Jacz., Bull. Herb. Boissier 2: 663 (1894) Facesoffungi number: FoF 00844 Saprobic on dead twigs attached to trees, appearing as black dots, below small bumps on the bark. Sexual morph: Ascomata perithecial, scattered, solitary or aggregated, immersed, depressed globose, coriaceous, black, ostiolate, papillate. Ostiolar papilla short, cylindrical, wide at the apex, even with the bark surface, periphysate. Peridium comprising narrow, compressed, light brown, thin-walled, cells of textura angularis at the base and thick-walled, light brown to hyaline, isodiametric cells around the ostiole. Hamathecium comprising numerous, apically narrow, basally wide, hyaline Fungal Diversity Fungal Diversity paraphyses. Asci 8-spored, unitunicate, clavate to fusoid, short pedicellate, apically rounded, with a J+ or J– apical ring. Ascospores biseriate or partially uniseriate, hyaline, broadly ellipsoid, oblong or narrowly clavate, apiosporous with a rounded to subconical small lower cell, straight or curved, thick-walled, smooth-walled. Asexual morph: Hyphomycetous, Setae erect, dark brown, straight to flexuous, arising from superficial hyphae, branched at base, subcylindrical, tapering to obtuse apex, 3–7-septate, basal cell slightly swollen. Conidiophores subcylindrical to setiform, with radially lobed basal cells, pale brown, smooth, arising from superficial mycelium, straight to flexuous, 1–10-septate. Conidiogenous cells terminal or lateral, polyblastic, subcylindrical to somewhat clavate, pale brown, smooth, with 1–4 denticulate loci. Conidia pale brown, lageniform to rhomboid, distal end free, proximal end rostrate, with a subhyaline transverse band at equatorial zone, smooth-walled, guttulate, aseptate. Pseudomassaria chondrospora (Ces.) Jacz., Bull. Herb. Boissier 2: 663 (1894). Facesoffungi number: FoF 00845; Fig. 33. Saprobic on dead twigs of Tilia cordata Mill.., appearing as black spots, with long fissures on the host surface. Sexual morph: Ascomata 610–625μm high, 765–800μm diam., (x =620×775μm, n=20) solitary or aggregated, scattered, immersed, depressed globose to ellipsoid, brown, short papillate, ostiolate. Ostiolar papillashort, cylindrical, wide at the apex, even with the bark surface, inner layer of ostiolar canal covered with hyaline, periphyses. Peridium 25–45μm wide, (x = 45μm, n=20), comprising 5–7 layers of narrow, compressed, light brown, thin-walled cells of textura angularis at the base and thick-walled, light brown to hyaline, isodiametric ce lls around the ostiole. Hamathecium comprising 10–17 μm wide (x = 12 μm, n = 20), septate, branched paraphyses. Asci 200–250 × 70–80μm (x =226×77μm, n=20), 8-spored, unitunicate, broadly ellipsoidal, clavate to fusoid, short pedicellate, apex rounded, with an indistinct, J- apical apparatus. Ascospores 65–100 × 20–35 μm (x = 82× 26.5 μm, n = 20), biseriate or partly obliquely uniseriate, hyaline, broadly ellipsoid, oblong or clavate, apiosporous, with a rounded to subconical, small lower cell, straight or rarely curved, thick-walled, smooth-walled, granulate. Asexual morph: Undetermined. Culture characters: Cultures growing on MEA reaching 1 cm. diam. at 18 °C, after 10 days. Slow growing, irregular colony, margin cream yellow, central region gray and tightly adpressed to the media, outer region woolly, serrate to smooth margin. Material examined: ITALY, Province of Forlì-Cesena, Premilcuore, Fiumicello, on branch of Tilia cordata, 24 April 2013, Erio Camporesi, IT 1200 (MFLU 15–0729, reference specimen designated here); living culture, MFLUCC Fig. 32 Melogramma campylosporum (S, F123341). a Herbarium packet. b Host. c, d, f Stromata on wood surface. e Periphyses of ascomata. g Peridium. h–l Asci. m Paraphyses. n–q Ascospores. Scale bars: c, d, f=500 μm, e, g–m=30 μm, n–q=15 μm 15–0545, ICMP. Ibid. MFLU 15–0729bis, MFLU 15– 0729tris. Notes: Pseudomassaria chondrospora is the generic type of Pseudomassaria. However we could not locate the type material of Pseudomassaria chondrospora and there is no information of host and locality. However, Jaklitsch and Vo g l m a y r ( 2 0 1 2 ) i l l u s t r a t e d a n d d e s c r i b e d t h e Pseudomassaria chondrospora (WU 31321) collected from twigs of Tilia platyphyllos and we compared morphological characters of our collection with that illustration. As well as Mega blast search results for LSU of our strain gives closest match as Pseudomassaria chondrospora (PC1, JF440982; Identity=1055/1097 (96 %), Gaps=20/1097 (1 %)). Hence considering both morphology and phylogeny and lacking of information about host and locality, we designate our collection as a reference specimen of P. chondrospora (sensu Ariyawansa et al. 2014). Pseudomassaria corni (Sowerby) Arx, Ber. schweiz. bot. Ges. 62: 349 (1952) Basionym: Sphaeria corni Sowerby, Col. fig. Engl. Fung. Mushr. 3: pl. 370 (1803) Facesoffungi number: FoF 00846; Fig. 34 Saprobic on dead twigs of Cornus sanguine L., appearing as black, shiny, dots on host surface. Sexual morph: Ascomata 195–240μm high, 280–315μm diam., (x =222× 299μm, n=20), solitary or aggregated in groups, scattered, immersed, globose, brown, ostiolate. Ostiole short, wide, cylindrical. Peridium comprising two stratum, outer 5–6 layers of dark brown, cells of textura globulosa, inner 1–2 layers of narrow, compressed, hyaline to light brown, thin-walled, cells of textura angularis. Hamathecium comprising very few, hyaline, septate paraphyses. Asci 55–80×12–15μm (x =67× 13μm, n=20), 8-spored, unitunicate, clavate to fusiform, short pedicellate, apex rounded with indistinct, J- apical apparatus. Ascospores 16.5–17.5×6–7.5μm (x =17×6.5μm, n=20), biseriate to multiseriate, hyaline, oblong or clavate, ends pointed, unicellular, thick-walled, smooth-walled. Asexual morph: Undetermined. Culture characters: Cultures growing on MEA at 18 °C, after 14 days reaching 2 cm, with circular, yellowish white, colonies, tightly adpressed to the media, woolly, loosely arranged, margin smooth. Material examined: ITALY, Province of Forlì-Cesena [FC], Santa Sofia, Camposonaldo, on Cornus sanguine, 5 May 2013, Erio Camporesi, IT 1219 (MFLU 15–0728, reference specimen designated here), living culture, MFLUCC 15–0544 = ICMP. Ibid. MFLU 15–0728bis, MFLU 15– 0728tris, paratypes). Fungal Diversity Fungal Diversity Fig. 33 Pseudomassaria chondrospora (reference specimen). a, b Appearance of ascomata on host substrate. c Paraphyses. d Cross section of ascoma. e Peridium. f–i Asci. j–p Ascospores. q Culture from upper surface. r Culture from lower surface. Scale bars: a, b=500 μm, d=100 μm, c, e–p=10 μm Vialaeaceae P.F. Cannon, Mycol. Res. 99 (3): 368 (1995) Facesoffungi number: FoF 00686 Biotrophic, parasitic or endophytic on dead plant matter, often on dead twigs attached to trees. Sexual morph: Pseudostroma (pseudoclypeus) absent or if present, immersed, ellipsoidal, as slightly raised black dots on substrate. Ascomata perithecial, solitary or aggregated, immersed, globose to subglobose, coriaceous, ostiolate, with immersed, Fungal Diversity Fig. 34 Pseudomassaria corni (reference specimen) a, b Ascomata on substrate. c Cross section of ascoma. d Peridium. e–h Asci. i–m Ascospores. Scale bars: a=500 μm, b=200 μm, c=20 μm, d–m=10 μm periphysate, ostiolar canal, sometime converging and fusing into the centre of the pseudostroma and opening through a common ostiole, black to brown. Papilla long, sometimes distal end curving towards the substrate or immersed ostiolar canal Periphyses hyaline, filamentous. Peridium comprising several layers of slightly flattened, strongly melanized, thickwalled cells of textura globulosa or textura angularis with peridial pores between cells. Hamathecium comprising few, filiform, rarely branched, septate, hyaline, paraphyses sometimes apically swollen and shorter than asci. Asci 8-spored, unitunicate, cylindrical, apex rounded, short pedicellate, apex obtuse or truncate, with a J+ sub-apical ring. Ascospores biseriate, triseriate to fasciculate, sometimes weakly helically coiled, 1–3-septate, hyaline, with two fusiform end cells, tapering gradually to acute ends and towards the central portion, joined by a narrow, long isthmus. Asexual morph: Ceolomycetous. Conidiomata pycnidia, superficial, solitary, scattered, globose, with slimy, shining spore mass and basal mycelium forming thick, black strands. Conidiophores erect, branched, septate, hyaline. Conidiogenous cells phialidic, integrated, in small whorls, lageniform to cylindrical, hyaline. Conidia oblong to ellipsoidal, one-celled, hyaline smoothwalled, with a truncate abscission scar. Type: Vialaea Sacc., Bull. Soc. mycol. Fr. 12: 66 (1896) Notes: Vialaeaceae was used by Schrantz (1960) as “Vialaeacees” and formally established as Vialaeaceae by Cannon (1995). This is a monotypic family comprising Vialaea (Maharachchikumbura et al. 2015). Fungal members of this family are endophytes or weak pathogens causing cankers on young twigs and on older branches of trees (Senanayake et al. 2014). Vialaea Sacc., Bull. Soc. mycol. Fr. 12: 66 (1896) Fungal Diversity Facesoffungi number: FoF 000011 Biotrophic, parasitic or endophytic in twigs of trees, eventually saprobic. Sexual morph: Pseudostromata lacking or if present, immersed in host tissues, with brown to black tissues around immersed neck. Ascomata perithecial, immersed, solitary or aggregated in circular groups with ostioles converging in their centre, globose or subglobose, coriaceous, somewhat flattened, black to brown. Ostiolar canal elongate, dark brown to black, periphysate. Peridium composed of several layers of slightly flattened, strongly melanized, thick-walled cells of textura globulosa or textura angularis with peridial pores between cells. Hamathecium comprising poorly developed, thin-walled, hyaline, septate, paraphyses, shorter than the asci. Asci 8-spored, unitunicate, cylindrical, sometimes tapering towards the apex or base, short pedicellate or sessile, thin-walled except at the apex, apex obtuse or truncate, subapical ring, J+, subconical. Ascospores biseriate, triseriate to fasciculate, sometimes weakly helically coiled, 1–3-septate, hyaline, with two fusiform end cells, joined by a narrow, long isthmus. Asexual morph: Coelomycetous, Conidiomata pycnidial, superficial, solitary, globose. Conidiophores erect, branched, septate, hyaline. Conidiogenous cells enteroblastic, annelledic, proliferating percurrently to produce small, hyaline, ellipsoidal conidia. Conidia oblong to fusiform, onecelled, hyaline and smooth-walled. Vialaea mangiferae Senan. & K.D. Hyde, Sydowia 66 (1): (2014); Fig. 35 Notes: This species was introduced in Senanayake et al. (2014) and we illustrate both sexual and asexual morph here. Material examined: THAILAND, Chiang Rai Province, Muang District, near Bandu, Baan Khuakhae, at 31 M. 17, (19° 59’ 52.05” N; 99° 49’ 25.15” E), on twigs of Mangifera indica, 15 November 2012, K.D. Hyde, CHUNI001 (MFLU 13–0342, holotype. Notes: Vialaea was introduced and is typified by Vialaea insculpta (Fr.) Sacc. (Saccardo 1896) and placed in Amphisphaeriaceae (Xylariales) based on the J+ apical ring (Shoemaker et al. 2013). Cannon (1995) excluded Vialaea from Amphisphaeriaceae and proposed a new family, Vialaeaceae, for Vialaea species, based on the stromata, ascomata and ascal characteristics, as well as nutritional strategy as a weak pathogen. Vialaea comprises of V. insculpta (Fr.) Sacc., V. minutella Petr., and Vialaea mangiferae Senanayake & KD. Hyde (Senanayake et al. 2014). Vialaea bambusae Hara and V. ingae Rehm were excluded from the genus by Cannon (1995). The above placements were based on morphology and LSU gene sequences analysis has also shown that Vialaeaceae is a distinct family in the order Xylariales (Shoemaker et al. 2013; McTaggart et al. 2013; Senanayake et al. 2014; Maharachchikumbura et al. 2015). Xylariaceae Tul. & C. Tul. [as ‘Xylariei’], Select. fung. carpol. (Paris) 2: 3 (1863) Index fungorum number: IF81528, Facesoffungi number: FoF00070 Saprobic, pathogenic, parasitic or endophytic in wood, leaves and fruits, dung inhabitants, and associated with insect vectors. Sexual morph: Stromata extremely variable in size, shape and colour, erect or applanate or effused–pulvinate or sometimes rudimentary, arising singly or aggregated, with 1 to many ascomata, ostiolate, with or without extractable stromatal pigments, bipartite or unipartite. Ascomata variable in size and shape, globose-pyriform, embedded in the stroma, single or multi-layered. Hamathecium comprised of hyphoid, filamentous, septate paraphyses embedded in a gelatinous matrix. Asci 4–8-spored, unitunicate, cylindrical to clavate, pedicellate, apically rounded, with or without a J+ or J– apical apparatus or with apical thickenings. Ascospores uniseriatebiseriate (except for the genus Phylacia), brown to black, rarely hyaline, 1–2-celled, variously-shaped, mostly with a germ slit. Perispore dehiscent or lacking smooth or with patterns. Asexual morph: Two major types of asexual morph reported are nodulisporium-like and geniculosporium-like as defined by Bitzer et al. (2008), featuring hyaline to light brown, smooth, branched conidiophores bearing hyaline, roughened or smooth, ellipsoidal conidia. Several other hyphomycetous and coelomycetous genera have been linked to Xylariaceae (Crous et al. 2014). Type: Xylaria Hill ex Schrank, Baier. Fl. (München) 1: 200 (1789). Type species: Xylaria hypoxylon (L.) Grev., Fl. Edin.: 355 (1824) Possible synonymy: See Index Fungorum (2015) Notes: The family Xylariaceae was introduced by Tulasne and Tulasne (1863) using the term “Xylariei”. The rank was not certain as it did not address the family concept (Stadler et al. 2014). Numerous studies have since contributed to the knowledge of the family (i.e., Dennis 1957, 1961; Læssøe et al. 1989; Miller 1961; Ju and Rogers 1996) and recent studies have revised the family using ultra-structural data of ascal and ascospore characters. Chemotaxonomic data have also played an important role in the identification and characterization of the stromatic Xylariaceae (Whalley and Edwards 1995; Bitzer et al. 2008; Stadler et al. 2001). Ju and Rogers (1996) used stromatal pigments to characterize Hypoxylon, while Stadler et al. (2014) used a polyphasic taxonomic approach in their world monograph of Daldinia. Dennis (1961) was the first author to attempt the segregation of the Xylariaceae into three subfamilies, “Hypoxyloideae”, “Thamnomycetideae” and “Xylarioideae”. However, this segregation was still based on generic concepts that heavily relied on stromatal macromorphology, disregarding the asexual states, and does in no way relate to the modern concepts of the family. In addition, the subfamilies were not validly published (cf. Bitzer et al. 2008), and the “Thamnomycetideae” sensu Dennis later proved untenable Fungal Diversity Fig. 35 Vialaea mangiferae (holotype). a Herbarium specimen. b Appearance of ascomata on substrate. c Horizontal cross section of ascomata. d Vertical cross section of ascomata. e Peridium. f Paraphyses. g J+, subapical apparatus of ascus. h–j Asci. k–n Ascospores. o Surface view of culture on MEA. p Reverse view of culture on MEA. q Conidiomata. r Conidia attached to conidiogenous cells. s Conidia. Scale bars: b=200 μm, c=100 μm, d=200 μm, f, g= 50 μm, h, n=20 μm, q=1 mm, r, s =10 μm as it was found out by a polyphasic approach that the affinities of Thamnomyces s. str. are with Daldinia and allies whereas other species of Thamnomyces are related to Xylaria (Stadler et al. 2010b) . Nevertheless, some authors have used the remaining “subfamilies” in recent work, discriminating the taxa with “nodulisporium-like anamorphs” sensu Ju and Rogers (1996) (i.e., the hypoxyloid Xylariaceae) from those with “geniculosporium-like anamorphs” (i.e., Xylaria and allies). These subfamilies have been used in subsequent studies (Daranagama et al. 2014, 2015; Hsieh et al. 2005, 2010; Stadler et al. 2010a, c, 2013; Tang et al. 2009). Several polythetic and molecular phylogenetic studies (Daranagama et al. 2015; Maharachchikumbura et al. 2015; Stadler et al. 2010c) have indicated the possible need for the separation of “Hypoxyloideae” and “Xylarioideae” as separate families. However, molecular data are so far only available for a small fraction of the existing species and no reliable data are available for several genera. Therefore, the above classification is widely based on a combination of molecular data and morphological features relating to the type of asexual morph (cf. Stadler et al. 2013). Xylaria Hill ex Schrank, Baier. Fl. (München) 1: 200 (1789). Facesoffungi number: FoF00696 Saprobic on dead wood. Sexual morph: Stromata extremely variable in size and shape, simple to branched from Fungal Diversity the base, nearly sessile or arising from long rooted stipes, whitish when immature, gradually turning silvery grey and eventually black, longitudinally furrowed delimiting narrow strips, perithecial contours most often inconspicuous in welldeveloped stromata, leathery, black, interior solid, homogeneous, white to cream coloured, with a slightly darker core, stipes cylindrical to strap-like, black, with a hairy-tomentose broadened base. Ascomata subglobose, immersed to slightly exposed. Ostioles raised-discoid, grey brown to black, often with a low conical papilla at the center. Hamathecium comprising sparse, hypha-like, hyaline, septate paraphyses. Asci unitunicate, (6–) 8-spored, cylindrical, long pedicellate, usually with a J+, tubular, apical apparatus. Ascospores overlapping uniseriate in the ascus, ellipsoid-inequilateral, with narrowly to broadly rounded ends, medium brown, smoothwalled, some with a fugacious cellular appendage usually disappearing at maturity, usually with two large guttules, with a conspicuous straight germ slit, 1/2–4/5 of spore-length on the flattened side. Asexual morph: Geniculosporium or geniculosporium-like. (Ju and Rogers 1996; Stadler et al. 2014). Type species: Xylaria hypoxylon (L.) Grev., Fl. Edin.: 355 (1824) (Fournier et al. 2011; Persoh et al. 2009; Stadler et al. 2014) = Clavaria hypoxylon L., Sp. pl. 2: 1182 (1753) Notes: Xylaria is the largest and the type genus in the family Xylariaceae with more than 700 epithets listed in Index Fungorum (2015). Most Xylaria species are characterised by massive stromata which can varied in colour, shape and size based on the location, cylindrical asci with apical apparatus and dark ascospores unique to the family as well as their geniculosporium-like asexual morphs (Ju and Rogers 1996; Stadler et al. 2013). Stromata of most of the known Xylaria species occur on decayed dicotyledonous wood, fewer on monocotyledons, fruits, seeds and fallen leaves. Interestingly, a number of Xylaria species that comprise the subgenus Pseudoxylaria are associated with termite nests mainly of macrotermitine termites, rather than plants. They form a monophyletic clade in an otherwise polyphyletic mega-genus (Hsieh et al. 2010). Hypoxyloideae Hypoxyloideae or hypoxyloid Xylariaceae have nodulisporium–like asexual morphs (Ju and Rogers 1996). The genus Nodulisporium was introduced by Preuss (1849), and is typified by Nodulisporium ochraceum Preuss. Stadler et al. (2013) stated that N. ochraceum may be associated with Hypoxylon howeanum Peck. There are 27 generic names listed in Stadler et al. (2013) which have been included in the subfamily Hypoxyloideae. Xylarioideae The subfamily Xylarioideae has conidiogenous structures resembling those of Geniculosporium (Ju and Rogers 1996; Stadler et al. 2013). Chesters and Greenhalgh (1964) introduced Geniculosporium with Geniculosporium serpens Chesters & Greenh. as the type species (whch is actually the asexual state of Nemania serpens. hence the name is now obsolete). Stadler et al. (2013) have listed 38 asexual and sexual genera that can be included in the subfamily Xylarioideae. The phylogeny of the Xylariaceae is currently being examined in a separate study, using morphological and chemotaxonomic data and a multi-gene genealogy. Therefore, no detailed information is being provided in the current paper. Acknowledgments S. Maharachchikumbura thanks the Featured microbial resources and diversity investigation in Southwest Karst area (2014FY120100) for funding. The authors extend their sincere appreciations to the Deanship of Scientific Research at King Saud University for its funding this Prolific Research Group (PRG-1436-09). Kevin D. Hyde thanks the Chinese Academy of Sciences, project number 2013T2S0030, for the award of Visiting Professorship for Senior International Scientists at Kunming Institute of Botany. Y.P. Xiao and T.C. Wen are grateful to The National Natural Science Foundation of China (No.31460012 & No.31200016). Shi-Ke Huang and J.C. Kang are grateful to the Agricultural Science and Technology Foundation of Guizhou Province Nos. 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