available online at www.studiesinmycology.org
StudieS in Mycology 72: 1–401.
The genus Cladosporium
K. Bensch1,2*, U. Braun3, J.Z. Groenewald2 and P.W. Crous2,4,5
1
Botanische Staatssammlung München, Menzinger Straße 67, D-80638 München, Germany; 2CBS-KNAW Fungal Biodiversity Centre, P.O. Box 85167, NL-3508 AD Utrecht,
The Netherlands; 3Martin-Luther-Universität, Institut für Biologie, Bereich Geobotanik und Botanischer Garten, Herbarium, Neuwerk 21, D-06099 Halle (Saale), Germany;
4
Microbiology, Department of Biology, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands; 5Wageningen University and Research Centre (WUR), Laboratory of
Phytopathology, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands
*Correspondence: Konstanze Bensch, schubert@bsm.mwn.de
Abstract: A monographic revision of the hyphomycete genus Cladosporium s. lat. (Cladosporiaceae, Capnodiales) is presented. It includes a detailed historic overview of
Cladosporium and allied genera, with notes on their phylogeny, systematics and ecology. True species of Cladosporium s. str. (anamorphs of Davidiella), are characterised by
having coronate conidiogenous loci and conidial hila, i.e., with a convex central dome surrounded by a raised periclinal rim. Recognised species are treated and illustrated with
line drawings and photomicrographs (light as well as scanning electron microscopy). Species known from culture are described in vivo as well as in vitro on standardised media
and under controlled conditions. Details on host range/substrates and the geographic distribution are given based on published accounts, and a re-examination of numerous
herbarium specimens. Various keys are provided to support the identiication of Cladosporium species in vivo and in vitro. Morphological datasets are supplemented by DNA
barcodes (nuclear ribosomal RNA gene operon, including the internal transcribed spacer regions ITS1 and ITS2, the 5.8S nrDNA, as well as partial actin and translation
elongation factor 1-α gene sequences) diagnostic for individual species. In total 993 names assigned to Cladosporium s. lat., including Heterosporium (854 in Cladosporium
and 139 in Heterosporium), are treated, of which 169 are recognized in Cladosporium s. str. The other taxa are doubtful, insuficiently known or have been excluded from
Cladosporium in its current circumscription and re-allocated to other genera by the authors of this monograph or previous authors.
Key words: biodiversity, cladosporioid hyphomycetes, Davidiella anamorphs, generic concept, keys, phylogeny, species concept, status quo, taxonomy.
Taxonomic novelties: Cladosporium allicinum (Fr. : Fr.) Bensch, U. Braun & Crous, comb. nov., C. astroideum var. catalinense U. Braun, var. nov., Fusicladium tectonicola
(Yong H. He & Z.Y. Zhang) U. Braun & Bensch, comb. nov., Septoidium uleanum (Henn.) U. Braun, comb. nov., Zasmidium adeniae (Hansf.) U. Braun, comb. nov., Zasmidium
dianellae (Sawada & Katsuki) U. Braun, comb. nov., Zasmidium lythri (Westend.) U. Braun & H.D. Shin, comb. nov., Zasmidium wikstroemiae (Petch) U. Braun, comb. nov.
Published online: 22 May 2012; doi:10.3114/sim0003.
Studies in Mycology
INTRODUCTION
Species of Cladosporium are cosmopolitan in distribution and
commonly encountered on all kinds of plant, fungal and other
debris, are frequently isolated from soil, food, paint, textiles and
other organic matters or colonise as secondary invaders leaf
lesions caused by plant pathogenic fungi (Ellis 1971, 1976).
Conidia of Cladosporium species also represent the most common
fungal component isolated from air (Farr et al. 1989, Flannigan
2001, Mullins 2001). With their small conidia, usually formed in
branched chains, they are well adapted to be spread easily in
large numbers over long distances. Other species of this genus
are plant pathogenic, i.e., they are causal agents of leaf spots and
other lesions (Schubert 2005b), or they occur as hyperparasites
on other fungi (Heuchert et al. 2005). Cladosporium species are
also known to be common endophytes (Riesen & Sieber 1985,
Brown et al. 1998, El-Morsy 2000) as well as phylloplane fungi
(Islam & Hasin 2000, de Jager et al. 2001, Inacio et al. 2002, Stohr
& Dighton 2004, Levetin & Dorseys 2006). Some species have a
medical relevance in clinical laboratories and may cause allergic
lung mycoses (de Hoog et al. 2000). Because many Cladosporium
species are cosmopolitan, are agents of decay, deterioration, or a
cause of allergy or even plant or animal disease, and are often of
high environmental impact, the genus is of interest to researchers
in a wide variety of disciplines.
Moreover, Cladosporium is one of the largest and most
heterogeneous genera of hyphomycetes, currently encompassing
more than 772 names (Dugan et al. 2004). Until relatively recently,
all kinds of unrelated dematiaceous hyphomycetes characterised
by having amero- to phragmosporous conidia formed in acropetal
chains had been assigned to Cladosporium s. lat., creating a
considerable obstacle to a monograph of this genus. Therefore,
Cladosporium was considered in most urgent need of critical
revision by the International Commission on the Taxonomy
of Fungi (Hawksworth 1986). Various authors discussed the
heterogeneity of Cladosporium s. lat. and proposed new, more
natural circumscriptions of this genus (e.g., von Arx 1981, 1983,
Morgan-Jones & Jacobsen 1988, McKemy & Morgan-Jones
1990, Morgan-Jones & McKemy 1990, Braun 1995b, Partridge
& Morgan-Jones 2002, 2003). Based on re-assessments of
morphological features and molecular data, various groups of
cladosporioid anamorphs could be excluded from Cladosporium
s. str., e.g., human pathogenic species [Herpotrichiellaceae] (de
Hoog et al. 1995, Masclaux et al. 1995), Venturia anamorphs
[Venturiaceae] (Schubert et al. 2003, Beck et al. 2005) and several
heat-resistant fungi [Teratosphaeriaceae] (Seifert et al. 2004).
David (1997) revised Cladosporium species previously referred
to as Heterosporium and, using a scanning electron microscopic
approach, demonstrated that the genus Cladosporium is wellcharacterised and easily recognisable by its unique structure of
Copyright CBS-KNAW Fungal Biodiversity Centre, P.O. Box 85167, 3508 AD Utrecht, The Netherlands.
You are free to share - to copy, distribute and transmit the work, under the following conditions:
Attribution:
You must attribute the work in the manner speciied by the author or licensor (but not in any way that suggests that they endorse you or your use of the work).
Non-commercial:
You may not use this work for commercial purposes.
No derivative works: You may not alter, transform, or build upon this work.
For any reuse or distribution, you must make clear to others the license terms of this work, which can be found at http://creativecommons.org/licenses/by-nc-nd/3.0/legalcode. Any of the above conditions can be waived if you get
permission from the copyright holder. Nothing in this license impairs or restricts the author’s moral rights.
1
BenSch et al.
the conidiogenous loci and conidial hila, which he classiied as
coronate, i.e., composed of a central convex dome surrounded
by a raised periclinal rim. David’s (1997) approach was critical for
enabling a revision of the genus Cladosporium. A few years later, the
irst attempts were made to revise and monograph Cladosporium
s. lat. (Crous et al. 2001). Braun et al. (2003) published results
of the irst molecular examinations of Cladosporium s. lat., clearly
conirming the strong heterogeneity of this genus. Furthermore,
they showed that the teleomorphs of Cladosporium s. str. species,
previously referred to Mycosphaerella, warrant placement in a
new separate genus, Davidiella. Although no clear morphological
differences were reported between Davidiella and Mycosphaerella,
a further study by Aptroot (2006) found ascospores of Davidiella
to have characteristic irregular cellular inclusions (lumina),
which are absent in species of Mycosphaerella, along with
periphysoids and pseudoparaphyses (Schubert et al. 2007b).
Furthermore, higher order phylogenetic studies, which employed
DNA sequence data of four loci (SSU nrDNA, LSU nrDNA, EF-1α,
RPB2), revealed that species of Davidiella cluster in a separate
family [Cladosporiaceae (= Davidiellaceae)] from species of
Mycosphaerella (Mycosphaerellaceae), with both families residing
in Capnodiales (Dothideomycetes), and not Dothideales as always
presumed (Schoch et al. 2006, 2009a, b, Crous et al. 2009b). In
a series of papers, various Cladosporium species have been reexamined and reassessed, based on the new circumscription of the
genus (Schubert & Braun 2004, 2005a, b, 2007, Schubert 2005a,
Schubert et al. 2006, Braun & Schubert 2007, Braun et al. 2008a).
Schubert (2005b) monographed foliicolous Cladosporium species,
and Heuchert et al. (2005) published a morphotaxonomic treatment
of fungicolous taxa. These papers were important steps towards a
modern revision of Cladosporium. However, the recently published,
comprehensive revisions of numerous cladosporioid genera,
based on molecular sequence analyses, cultures and morphology
(Crous et al. 2006, 2007a) provided the inal necessary component
required for the preparation of the present taxonomic study.
In the present treatment, a survey of Cladosporium s. lat. is
given, i.e., the current knowledge about the taxonomy of true species
of Cladosporium (s. str.) is relected, and excluded taxa, previously
assigned to Cladosporium s. lat., are listed with reference to their
current status and generic afinity. Taxa for which type specimens
or any other authentic collections could not be traced, do not exist,
or have not been available on loan are separately listed. Accepted
species are described in vivo and, if data are available, also in
vitro. However, only a small fraction of Cladosporium species is
known from culture. Furthermore, the recently published revisions
of Cladosporium herbarum s. lat. (Schubert et al. 2007b), C.
sphaerospermum s. lat. (Zalar et al. 2007, Dugan et al. 2008) and
C. cladosporioides s. lat. (Bensch et al. 2010) clearly showed that
these saprobic species have to be considered as heterogeneous
complexes, composed of several genetically and morphologically
distinguished species. Unfortunately, the examination of the
diversity, phylogeny and taxonomy of Cladosporium s. str. is still
in an initial phase, and thus the present work should be seen as a
handbook relecting on the current taxonomic status quo.
HISTORICAL OVERVIEW
Comprehensive reviews of the history of Cladosporium have been
provided by David (1997), Heuchert et al. (2005) and Schubert
(2005b), and are briely discussed below. The genus Cladosporium
2
was established in 1816 by Link, who described it as follows:
“Thallus e loccis caespitosis, erectis simplicibus aut subramosis,
apicibus in sporidia secedentibus. A Sporothricho et Oidio differt
loccis non intricatis, ab Acladio, sporidiis apici primum innatis, dein
delabentibus”. Link (1816) included C. herbarum [introduced by
Persoon (1794) as Dematium herbarum and later reclassiied by
Link (1809) as Acladium herbarum], C. abietinum [= Trentepohlia
abietina, ide Hughes (1958)], C. atrum [= sterile fungus, ide
Hughes (1958)] and C. aureum [= ?Trentepohlia aurea, lectotype
of Trentepohlia]. Clements & Shear (1931) proposed C. herbarum
as lectotype species, a decision followed by de Vries (1952) and
Hughes (1958). Cladosporium became rapidly established in
the literature, being used by Martius (1817), Nees (1817) and in
Fries’ “Systema mycologicum” (1821, 1832), and encompassed a
steadily growing number of species. Link (1824) described seven
species, Corda (1837) listed 15 species and Rabenhorst (1844) 23
species. This number grew to 110 in Saccardo (1886), who already
noted this genus as being problematic. The steady increase in taxa
continued, so that by 1931, 270 species had been described in
the genus and listed in the various volumes of Saccardo’s “Sylloge
fungorum”. Most of the original diagnoses of the species concerned
are very brief and imprecise. Although available information of
many of the older taxa has been rather meagre, description of new
species has continued unabatedly. Since 1950, more than 130
new species have been added (Morgan-Jones & McKemy 1990).
Prasil & de Hoog (1988) estimated Cladosporium to have around
540 species. A recently published checklist contains data for 772
Cladosporium names, i.e., valid, invalid, legitimate and illegitimate
species, varieties, formae as well as herbarium names (Dugan et
al. 2004). Reasons for this vast number of taxa probably reside
in the imprecise, wide circumscription of this genus in literature,
the strong morphological variability of most species, and the
occurrence of some species on a wide range of substrates.
De Vries (1952) examined Cladosporium in vitro and provided
descriptions of nine species with a further 13 species as an
appendix. Ellis (1971, 1976), who followed a very wide generic
concept, treated 43 species. Morgan-Jones and McKemy initiated a
series “Studies in the genus Cladosporium sensu lato”, in which they
dealt with selected species providing comprehensive descriptions
of their features in vivo and in vitro (Morgan-Jones & McKemy
1990, McKemy & Morgan-Jones 1990, 1991a–c). Descriptions
and an expanded key to the Cladosporium species available in
culture was provided by Ho et al. (1999), but the authors followed
a rather wide taxonomic concept, including species that belong to
other genera. Zhang et al. (2003) published a monograph of the
genera Cladosporium, Fusicladium and Pyricularia from China,
including numerous new Chinese Cladosporium species previously
published by Zhang and co-workers. Furthermore, they reported
numerous old species introduced in the 19th or early 20th century to
occur in China, but without having seen any type material of these
taxa, so that these names have probably often been misapplied.
Unfortunately, the access to type material and additional collections
cited in this work, which are deposited at MHYAU, was refused and
could therefore not be re-examined. Morphotaxonomic revisions
of foliicolous as well as fungicolous Cladosporium species have
recently been carried out by Schubert (2005b) and Heuchert et al.
(2005), respectively.
The status of the genus Heterosporium has been controversial.
Based on characteristically large, mostly phragmosporous conidia,
usually formed singly, and mostly rather coarse, fasciculate
conidiophores often emerging through stomata, several authors
considered Heterosporium a genus distinct from Cladosporium
the genuS Cladosporium
(e.g., von Arx 1983, McKemy & Morgan-Jones 1990). De Vries
(1952) concluded that a separation of the two genera based on the
formation and septation of conidia is impractical and not tenable,
since all kinds of transitions occur, and reduced Heterosporium to
synonymy with the latter genus, a treatment supported by Hughes
(1958). Ellis (1971, 1976) followed this arrangement and transferred
the names of the remaining accepted species of Heterosporium to
Cladosporium. Von Arx (1981) reinstated the use of Heterosporium
and emphasised that the recognition of Heterosporium was a irst
step towards delineating homogenous genera in the Cladosporium
complex. During the course of monographic studies in the genus
Heterosporium, David (1997) examined Cladosporium and
Heterosporium by means of scanning electron microscopy (SEM)
and clearly demonstrated that the conidiogenous loci and conidial
hila in the two genera are very similar, i.e., they are coronate with
a central convex dome and a raised periclinal rim. Based on these
results, he again placed Heterosporium in Cladosporium, and
proposed the combination Cladosporium subgen. Heterosporium.
Braun et al. (2003) conirmed this treatment based on molecular
data.
Masclaux et al. (1995), Untereiner (1997), Gerrits van den
Ende & de Hoog (1999), Untereiner & Naveau (1999), Untereiner
et al. (1999) and de Hoog et al. (2000) treated and revised former
human pathogenic Cladosporium species, using molecular and
physiological approaches. Based on comprehensive molecular
sequence analyses and morphological re-assessments, Braun
et al. (2003), Crous et al. (2006, 2007b–d), Schubert et al.
(2007a) and Seifert et al. (2004, 2007), revised various parts of
the heterogeneous complex of cladosporioid hyphomycetes,
demonstrating that numerous groups of species previously
referred to as Cladosporium have to be excluded since they do
not agree with Cladosporium s. str. [Capnodiales, Cladosporiaceae
(Schoch et al. 2006, 2009a, b, Crous et al. 2007b, 2009b)], neither
morphologically nor genetically.
GENERIC CONCEPT AND CIRCUMSCRIPTION OF
CLADOSPORIUM BASED ON MORPHOLOGY AND
PHYLOGENETIC DATA
Due to the very brief, imprecise circumscription of the genus
Cladosporium in the past, numerous supericially similar pigmented,
holoblastic hyphomycetes with amero- to phragmosporous conidia
formed in acropetal chains have been placed in Cladosporium s.
lat., which made this genus very heterogeneous and polyphyletic.
This heterogeneity has been recognised and discussed by several
authors (von Arx 1981, McKemy & Morgan-Jones 1990, David
1997).
The circumscription and delimitation of Cladosporium s. str. has
to be based on morphology and phylogeny of its type species, C.
herbarum. David (1997) pointed out that C. herbarum and other
genuine Cladosporium spp., including former Heterosporium spp.,
are well-characterised by having a unique type of conidiogenous
locus and conidial hilum that he classiied as coronate, i.e.,
with a central convex dome, surrounded by a raised periclinal
rim. Cladosporium herbarum and all other true Cladosporium
spp. had been, as far as known, considered anamorphs of the
ascomycete genus Mycosphaerella. Previous molecular studies
employing rDNA ITS sequence data (Crous et al. 2001) had
shown Cladosporium-like taxa clustering adjacent to the main
monophyletic Mycosphaerella clade, suggesting a position apart of
www.studiesinmycology.org
the latter genus. Braun et al. (2003) conirmed David’s (1997) new
circumscription of Cladosporium using molecular approaches, and
introduced the new genus Davidiella for the former Mycosphaerella
teleomorphs of Cladosporium s. str., since they formed a wellsupported sister clade of Mycosphaerella s. str., the latter
having cercosporoid anamorphs. Aptroot (2006) found additional
morphological characters for a better circumscription of Davidiella
and an easier delimitation against Mycosphaerella s. str., which
enabled him to refer some species of the former genus to Davidiella
in spite of the lacking Cladosporium anamorphs. In a comprehensive
phylogenetic treatment of Dothideomycetes based on the analysis
of four nuclear loci, Schoch et al. (2006) assigned Davidiella with
its Cladosporium anamorphs to the family Cladosporiaceae, which
they placed in Capnodiales, together with Mycosphaerellaceae. A
detailed morphological as well as molecular re-examination of C.
herbarum, the type species of Davidiella, has been published by
Schubert et al. (2007b).
Wirsel et al. (2002) and Park et al. (2004) carried out phylogenetic
studies within Cladosporium s. str. Wirsel et al. (2002) analysed
ITS data of strains isolated from common reeds in Germany,
compared them with sequences from GenBank and cultures
from the CBS (Utrecht, the Netherlands), and distinguished three
species, viz., C. herbarum, C. oxysporum and Cladosporium sp.
Beside ITS sequences, they generated two additional phylogenies,
viz., analyses based on the differentiation of the fungi by their
capacity to metabolise different carbon sources and a second
approach, using actin gene sequences, in which they discovered a
highly variable intron sequence. Species phylogenies based on this
protein-encoding gene exhibited higher resolution compared with
the ITS tree leading to further differentiation in terminal branches.
Furthermore, it could be shown that all strains with smooth conidial
surfaces clustered together, as did all isolates with rough-walled
conidia, thus relecting a possible division among plant-associated
Cladosporium species based on conidial ornamentation. However,
due to the limited dataset, including only few Cladosporium
species, a inal conclusion could not be drawn. Wirsel et al. (2002)
emphasised that multilocal analyses of the genome, based on
a larger number of isolates from different geographical regions
are necessary to redeine species borders within Cladosporium.
The weak resolution in phylogenetic trees based solely on ITS
sequences, insuficient for molecular delimitation at species rank,
was also pointed out by Braun et al. (2003). Schubert et al. (2007b)
carried out comprehensive molecular and morphological analyses
of the C. herbarum complex and demonstrated that a multilocal DNA
approach, based on ive genes, viz., rDNA ITS, actin, calmodulin,
translation elongation factor (1-α) and histone H3, resulted in a
much better resolution, appropriate for species analyses. In the
study carried out by Park et al. (2004), the sequences of the D1/D2
regions of the LSU rDNA genes and the ITS regions of the rDNA
were employed in order to establish molecular standards for the
demarcation of the common airborne species C. herbarum, C.
cladosporioides and C. sphaerospermum.
Based on re-assessments of morphological features and
molecular data, various groups of cladosporioid anamorphs could
be excluded from Cladosporium s. str., e.g., human pathogenic
species and Venturia anamorphs. Human pathogenic species, now
known to be species of Cladophialophora (teleomorph: Capronia,
Herpotrichellaceae) differ in their morphology (conidiophores
lacking or semi-macronematous, hila not coronate, less pigmented)
and physiology (inability to liquefy gelatine), differences conirmed
by molecular data (de Hoog et al. 1995, Untereiner 1997,
Untereiner et al. 1999, de Hoog et al. 2000). Fusicladium species
3
BenSch et al.
with catenate conidia have often been assigned to Cladosporium.
The revision of the genus Fusicladium (teleomorph: Venturia,
Venturiaceae) showed these species to be genuine members of
the latter genus (Schubert et al. 2003), a conclusion conirmed by
molecular data (Beck et al. 2005, Crous et al. 2007d). The structure
of the conidiogenous loci and hila of the genus Fusicladium is quite
distinct from those of Cladosporium in being more or less denticlelike, truncate to slightly convex (but non-coronate), unthickened,
not darkened or only somewhat darkened-refractive. Cladosporium
malorum (now Chalastospora gossypii) was shown to pertain to
Pleosporales (Braun et al. 2003, Crous et al. 2009a).
Other species originally placed in Cladosporium, proved
to be Mycosphaerella anamorphs belonging in Passalora,
Pseudocercospora, Stenella and Zasmidium respectively (Crous &
Braun 2003, Schubert & Braun 2005a, b, 2007, Braun & Schubert
2007). Species of the genus Passalora possess conspicuous, more
or less truncate, somewhat thickened and darkened-refractive
conidiogenous loci and hila, and Pseudocercospora is easily
distinguishable by its inconspicuous, unthickened and usually
not darkened conidiogenous loci (Crous & Braun 2003, Crous et
al. 2007c). The genus Stenella has traditionally been linked to
teleomorphs accommodated in Mycosphaerella (Crous & Braun
2003, Crous et al. 2004b, 2006). However, recent phylogenetic
studies have shown that Mycosphaerella is polyphyletic (Crous et
al. 2007b), and that many of these anamorph lineages represent
distinct genera with Mycosphaerella-like teleomorphs, clustering in
different families in Capnodiales (Crous et al. 2009a–c). Stenella is
a cercosporoid hyphomycete genus for species characterised by
having supericial, verruculose hyphae with solitary to aggregated
conidiophores, conidiogenous cells with conspicuous conidiogenous
loci (thickened, darkened and refractive), and conidia formed singly
or in chains, with thickened, darkened and refractive hila (Crous
& Braun 2003). In a subsequent study, Zasmidium with its type
species Z. cellare, proved to be the oldest name for Stenella-like
hyphomycetes within Mycosphaerellaceae (Arzanlou et al. 2007).
However, the type species of Stenella, S. araguata, clusters within
Teratosphaeriaceae, and differs in having pileate conidiogenous
loci (versus planate, cercosporoid loci in Zasmidium). Taxa with
a Zasmidium-like morphology appear to be paraphyletic within
Mycosphaerellaceae (Crous et al. 2009a, b), and these taxa should
be separated from Stenella, which has species with a different
scar structure and belongs to Teratosphaeriaceae (Braun et al.
2008a). Hence, Braun et al. (2010) and Kamal (2010) reallocated
most former Stenella species with planate, cercosporoid scars to
Zasmidium.
On account of morphological, molecular and ecological
features, Seifert et al. (2004) recently separated Cladosporium
staurophorum from Cladosporium s. str. and introduced the new
hyphomycete genus Devriesia (Teratosphaeriaceae, Capnodiales;
Crous et al. 2007b) to accommodate a group of ive heat-resistant
species that is also Cladosporium-like in its general morphology.
During the course of the morphotaxonomic revision of fungicolous
Cladosporium species (Heuchert et al. 2005), two additional
genera have been published, viz., Digitopodium with Digitopodium
hemileiae (basionym: Cladosporium hemileiae) as type species and
Parapericoniella with its type species Parapericoniella asterinae
(Figs 1, 2).
Cladosporium musae, a leaf-spotting fungus on banana leaves,
has recently been assigned to the new genus Metulocladosporiella,
an additional segregate of Cladosporium s. lat., which differs from
morphologically allied genera in having conidiophores which are
pigmented, frequently branched in a metula-like manner, with
4
much paler tips, forming paler, often subhyaline conidia. The
conidiogenous loci are subconspicuous to conspicuous, i.e.,
unthickened or almost so, but somewhat darkened-refractive (Crous
et al. 2006). The introduction of this genus was phylogenetically
supported, showing that it pertains to Chaetothyriales.
Comprehensive molecular and morphological re-examination
of further complexes of cladosporioid hyphomycetes showed that
numerous other taxa have to be excluded and reassessed (Crous
et al. 2007a). Some former Cladosporium species have been
assigned to the new genus Penidiella [anamorphs of Teratosphaeria,
Teratosphaeriaceae, Capnodiales (Crous et al. 2007b)], other
species have been placed in the new genera Rachicladosporium
(Capnodiales, incertae sedis), Toxicocladosporium (Capnodiales,
incertae sedis), Verrucocladosporium (Capnodiales, incertae sedis),
Hyalodendriella (Helotiales, incertae sedis), Ochrocladosporium
(Pleosporales, incertae sedis), and Rhizocladosporium (Helotiales,
incertae sedis) (Crous et al. 2007c). Schubert et al. (2007a)
introduced the new genus Dichocladosporium to accommodate
the Cladosporium occurring on Paeonia spp. [C. chlorocephalum,
C. paeoniae], for which, however, the older name Graphiopsis is
available (Braun et al. 2008a). Seifert et al. (2007) revisited the
creosote fungus (Amorphotheca resinae, anamorph Cladosporium
avellaneum) and the resin fungus (Hormodendrum resinae,
Cladosporium resinae, Sorocybe resinae), previously also confused
with Cladosporium (Figs 1, 2).
Generic concept and circumscription of
Cladosporium s. str.
Cladosporium Link, Ges. Naturf. Freunde Berlin Mag.
Neuesten Entdeck. Gesammten Naturk. 7: 37. 1816 : Fr.,
Syst. mycol. 3(2): 368. 1832.
≡ Sporocladium Chev., Fl. gén. env. Paris 1. 1826.
= Heterosporium Klotzsch, Herb. Viv. Mycol., Cent. I, No. 69. 1832, nom. inval.
≡ Heterosporium Klotzsch ex Cooke, Grevillea 5: 122. 1877.
= Myxocladium Corda, Icon. fung. 1: 12. 1837.
= Didymotrichum Bonord., Handb. Mykol.: 89. 1851.
= Acrosporella Riedl & Ershad, Sydowia 29: 166. 1977 [“1976”].
= Davidiella Crous & U. Braun, Mycol. Progr. 2(1): 8. 2003
[teleomorph name, see notes below].
= Mycosphaerella auct. p.p.
Type species: C. herbarum (Pers. : Fr.) Link (Clements & Shear
1931: 395). Lectotype: sine loco, sine dato (L 910.225-733),
selected by Prasil & de Hoog (1988). Epitype: the Netherlands,
Wageningen, isolated from Hordeum vulgare, 2005, P.W. Crous,
CBS H-19853, designated by Schubert et al. (2007b). Isoepitype:
HAL 2022 F. Ex-epitype cultures: CPC 12100 = CBS 121621, CPC
12178, 12179, 12181, 12183.
Lit.: de Vries (1952), Hughes (1958), Ellis (1971), Domsch et al.
(1980), Prasil & de Hoog (1988), David (1997), Samson et al.
(2000), Schubert et al. (2007b).
In vivo: Dematiaceous hyphomycetes; anamorphs of Davidiella.
Colonies punctiform to effuse, mostly olivaceous-brown to
blackish brown or with a grey-olivaceous appearance, velvety,
loccose or villose. Mycelium internal or external, supericial;
hyphae branched, septate, subhyaline to usually pigmented,
smooth, sometimes slightly rough-walled to verruculose.
Stromata absent to sometimes well-developed. Conidiophores
mononematous, usually macronematous, solitary, fasciculate, in
small to large fascicles, loosely to densely caespitose, usually
the genuS Cladosporium
Fig. 1 (Part 1). Cladosporium-like genera. A. Devriesia americana (CBS 117726; Crous et al. 2007c). B. Fusicladium proteae (CBS 130599; Crous et al. 2011a). C. Stenella
araguata (IMI 34905; Crous et al. 2007c). D. Zasmidium cellare (CBS 146.36; Arzanlou et al. 2007). E. Rachicladosporium luculiae (CBS 121620; Crous et al. 2007c). F.
Sorocybe resinae (DAOM 11381; Seifert et al. 2007). Scale bars = 10 µm.
erect, occasionally subdecumbent, decumbent or repent, straight
to lexuous, unbranched or branched, continuous to septate,
subhyaline to usually distinctly pigmented, smooth to verruculose,
proliferation holoblastic, occasionally enteroblastic (after a period
when growth has stopped and then resumed), usually sympodial,
rarely monopodial (sometimes leaving coarse annellations
www.studiesinmycology.org
from repeated enteroblastic proliferation). Conidiogenous
cells integrated, terminal or intercalary, monoblastic or usually
polyblastic, mostly sympodially proliferating, more or less
cylindrical, geniculate-sinuous or nodulose, sometimes with
unilateral swellings, conidiogenous loci usually conspicuous,
protuberant, composed of a central convex dome surrounded by a
5
BenSch et al.
Fig. 1 (Part 2). Cladosporium-like genera. A. Ochrocladosporium elatum (CBS 146.33; Crous et al. 2007c). B. Hortea thailandica (CBS 125423; Crous et al. 2009b). C.
Penidiella columbiana (CBS 486.80; Crous et al. 2007b). D. Ramularia cynarae (CBS 128912; Koike et al. 2011). E. Rhizocladosporium argillaceum (CBS 241.67; Crous et al.
2007c). Scale bars = 10 µm.
more or less raised periclinal rim (coronate), thickened, refractive
or barely to distinctly darkened; conidial formation holoblastic.
Conidia solitary or catenate, in unbranched or branched acropetal
chains, amero- to phragmosporous, shape and septation
variable, usually subglobose, ovoid, obovoid, ellipsoid, fusiform,
limoniform to cylindrical, aseptate or with several transverse
eusepta, rarely with a single longitudinal septum, subhyaline to
usually pigmented, smooth, verruculose, verrucose, echinulate,
6
cristate, hila protuberant, coronate, with a central convex dome
and raised periclinal rim, thickened, refractive to darkened;
microcyclic conidiogenesis often occurring.
In vitro: Stromata usually lacking. Conidiophores usually solitary,
arising terminally or laterally from plagiotropous or ascending
hyphae, often longer than in vivo. Micronematous conidiophores,
lacking in vivo, are often formed in culture. Conidial chains often
the genuS Cladosporium
Fig. 1 (Part 3). Cladosporium-like genera. A. Toxicocladosporium irritans (CBS 185.58; Crous et al. 2007c). B. Verrucocladosporium dirinae (CBS 112794; Crous et al. 2007c).
C. Hyalodendriella betulae (CBS 261.82; Crous et al. 2007c). D. Hormodendrum resinae (DAOM 41888; Seifert et al. 2007). E. Passalora californica (CBS 128857; Koike et al.
2011). F. Metulocladosporiella musae (CBS 121396; Crous et al. 2006). G. Amorphotheca resinae (DAOM 170427; Seifert et al. 2007). Scale bars = 10 µm.
www.studiesinmycology.org
7
BenSch et al.
Fig. 1 (Part 4). Cladosporium-like genera. A. Periconioid morph of Graphiopsis chlorocephalum (HAL 1924 F). B. Cladosporioid morph of Graphiopsis chlorocephalum (HAL
2011 F). C. Parapericoniella asterinae (IMI 11851b). D. Cladophialophora potulentorum (CBS 115144). E. Penidiella columbiana (CBS 486.80). F. Digitopodium hemileiae (BPI
426854). Scale bars = 10 µm.
8
the genuS Cladosporium
Saccharomyces cerevisiae Z73326
100 Athelia decipiens AY586632
Atheliales
Athelia epiphylla AY586633
Basidiomycota 100
78
Athelia neuhoffii GQ162812
Haplotrichum curtisii EU118629
100
Corticiales
Haplotrichum conspersum AY586657
Botryobasidium subcoronatum EU118607
81
Cantharellales
100 Botryobasidium subcoronatum EU909344
100 Seifertia azaleae EU030276
Beverwykella pulmonaria GU301804
Ascochyta pisi DQ678070
100
100 Phoma herbarum DQ678066
Didymella cucurbitacearum AY293792
95
bryoniae AB266850
Didymella
Pleosporales
100
94 Leptospora rubella DQ195792
Phaeosphaeria avenaria AY544684
Coniothyrium palmarum EU040225
78
76
Ochrocladosporium elatum EU040233
100 Ochrocladosporium frigidarii EU040234
Blumeria graminis f. sp. bromi AB022362
Erysiphales
Myxotrichum deflexum AY541491
Rhizocladosporium argillaceum EU040240
100
Hormoconis resinae EU040231
99
Hormoconis resinae EU040230
Helotiales
Bisporella citrina AY789385
Torrendiella eucalypti DQ195799
20 changes
Neofabraea alba AY064705
Hyalodendriella betulae EU040232
100
Sorocybe resinae EU030277
Lasallia pustulata AY300839
100 100
Cladophialophora australiensis EU035402
Cladophialophora chaetospira EU035406
91
ChaetoCapronia peltigerae HQ613813
82
Glyphium elatum AF346420
thyriales
100
100 Metulocladosporiella musae DQ008161
Metulocladosporiella musae DQ008162
100 Metulocladosporiella musicola DQ008159
100 Metulocladosporiella musicola DQ008160
Polyscytalum algarvense GQ303318
100 99
Polyscytalum fecundissimum EU035441
Phlogicylindrium eucalypti DQ923534
Xylariales
Pidoplitchkoviella terricola AF096197
76
97
Subramaniomyces fusisaprophyticus EU040241
74
100
Ascomycota
Parapleurotheciopsis inaequiseptata EU040235
100 Diaporthe phaseolorum AY346279
Diaporthales
Diaporthe angelicae AY196781
94
69
Taeniolella alta DQ377938
Chaetomium globosum AF286403
100
Aporothielavia leptoderma AF096186
Sordariales
72
Chaetomium homopilatum AF286404
72
Retroconis fusiformis EU040239
Fig. 2. The irst of 528 equally most parsimonious trees obtained from a heuristic search with 100 random taxon additions of the LSU sequence alignment using PAUP v. 4.0b10.
The scale bar shows 20 changes, and bootstrap support values from 1 000 replicates are shown at the nodes. Thickened lines indicate the strict consensus branches and orders
are indicated to the right of the tree. Generic names in green belong to Cladosporium s.str., those in red were in the past considered to be species of Cladosporium but were
subsequently renamed and those genera in blue are morphologically similar to Cladosporium and can be confused with it. The tree was rooted to Saccharomyces cerevisiae
(GenBank Z73326).
www.studiesinmycology.org
9
BenSch et al.
Fusicladium amoenum EU035425
Venturia hanliniana AF050290
100
Fusicladium carpophilum EU035426
Venturiales
67
Fusicladium effusum EU035430
54
Venturia inaequalis EU035437
Fusicladium convolvularum EU035428
100 Cladoriella eucalypti EU040224
Cladoriella eucalypti DQ195790
83
Aulographina eucalypti HM535600
100 Heteroconium eucalypti DQ885893
incertae sedis
53
Alysidiella parasitica DQ923525
60 Blastacervulus eucalypti GQ303302
Heteroconium kleinziense EF110616
99 Rachicladosporium cboliae GU214484
Rachicladosporium pini JF951165
98
Rachicladosporium
luculiae EU040237
91
Graphiopsis
chlorocephala
EU009457
83
Graphiopsis chlorocephala EU009458
Verrucocladosporium dirinae EU040244
Capnodiales:
Toxicocladosporium rubrigenum FJ790305
Cladosporiaceae
Toxicocladosporium irritans EU040243
98
Cladosporium cladosporioides DQ008145
59 85
Cladosporium uredinicola DQ008147
Cladosporium
allicinum DQ008149
100
63 Cladosporium cladosporioides DQ008146
100
Cladosporium iridis DQ008148
Periconiella
arcuata EU041836
90
98 Periconiella velutina EU041840
Zasmidium citri GU214502
Capnodiales:
100
Zasmidium cellare EU041878
87
Mycosphaerellaceae
99 Pseudocercospora vitis GU214483
Pseudocercospora paraguayensis AF309574
20 changes
Ramularia
aplospora EU040238
99
75
Ramularia endophylla AY490776
80
Passalora dahliae EU040236
100
Passalora fulva DQ008163
76
Penidiella aggregata JF499862
59
Penidiella rigidophora EU019276
98
Catenulostroma chromoblastomycosa EU019251
96
Stenella araguata EU019250
99
97
Penidiella ellipsoidea JF499863
Penidiella strumelloidea EU019277
Capnodiales:
Catenulostroma germania EU019253
Teratosphaeriaceae
50
Devriesia thermodurans EU040229
Devriesia shelburniensis EU040228
Devriesia staurophora DQ008150
Devriesia staurophora DQ008151
87
Fig. 2. (Continued).
longer than in vivo (species with solitary conidia are often capable
of forming conidial chains in culture).
Notes: In this monograph, we follow the spirit of the “Amsterdam
Declaration (Hawksworth et al. 2011), i.e. the proposal to apply
the principle “one fungus one name” and the impacts of decisions
made in Melbourne in July 2011 during the International Botanical
10
Congress (Hawksworth 2011), in particular the abolishment of Art.
59 dealing with pleomorphic fungi. As all names, anamorph-typiied
as well as teleomorph-typiied, will be nomenclaturally treated as
equal in future, the name Cladosporium has priority over Davidiella
at generic rank, and is also the more commonly used name in
literature. Therefore, Davidiella is cited here as a synonym of
Cladosporium. The abolishment of Art. 59 will come into effect only
the genuS Cladosporium
in January 2013. Nevertheless we prefer to follow the new rules in
advance. Nomenclatural consequences are not to be apprehended
as any combinations of teleotypiied names to anamorph-typiied
genera and vice versa result in valid names according to Art. 59 of
the old ICBN.
According to Lindau (1907), Mydonosporium is synonymous
with Cladosporium, a conclusion supported by de Vries (1952).
However, this conclusion could not be conirmed since type material
of its type species could not be examined since it is not preserved.
Fries (1949) reallocated the type species of Azosma (Corda 1831),
A. helminthosporioides, to Cladosporium, whereas Saccardo
and Traverso (Saccardo 1913a) assigned it to Macrosporium (=
Alternaria). The identity of Azosma remains doubtful and could
not be proven since type material is not preserved. Von Arx
(1983) considered Acroconidiella a synonym of Heterosporium
and reduced Stenella to synonymy with Cladosporium. However,
Acroconidiella possesses tretic conidiogenous cells and the
structure of the conidiogenous loci and hila within the genus
Stenella s. lat. is quite distinct from those of Cladosporium by being
pileate (Stenella s. str.) to planate (former species of Stenella s. lat.
now assigned to Zasmidium), i.e. in any case without dome and
raised rim. Hence, the two genera have to be retained as separate
genera (Crous & Braun 2003). Type material of Acrosporella (Riedl
& Ershad 1977) has recently been examined and shown to be a
synonym of Cladosporium (Braun 2009).
Subgeneric concepts
Attempts to divide Cladosporium into subgeneric units are
complicated due to the high degree of variability in the conidial size,
shape, septation, pigmentation, surface ornamentation as well as
in the conidiophore morphology and size of the particular species.
The habit of Cladosporium species is often signiicantly inluenced
by external impacts, e.g., substrate differences, climatic conditions,
diverse geographic inluences, etc. Colonies grown in vitro or in
moisture chambers are often strongly deviating from those found
on natural substrates.
Historical proposals to divide Cladosporium into smaller subunits
are unsatisfactory and have been barely practical (David 1997).
Saccardo (1886) introduced a system based on host preferences.
On the basis of ecological and morphological characteristics and
differences in vitro, Krangauz (1970) divided Cladosporium in three
subgenera (“Parasiticum”, “Eucladosporium” and “Saprophyticum”),
which were, however, not validly published. Von Arx (1983)
proposed four informal sections (excl. Heterosporium), again based
on ecological preferences. David (1997) introduced a subgeneric
classiication on the basis of morphological differences, recognising
the subgenera Heterosporium (conidia solitary or in short unbranched
chains, without ramoconidia, conidia rather uniform), Bistratosporium
(conidia in branched chains, ramoconidia present, walls of the conidia
distinctly two-layered) and Cladosporium (conidia in branched
chains, ramoconidia present, conidia polymorphous, walls always
one-layered). The latter subgenus was separated into the sections
Cladosporium (conidiophores proliferating) and Hormodendropsis
(conidiophores determinate, not proliferating). These two types
of conidiophore proliferation have previously been recognised by
de Vries (1952) and McKemy & Morgan-Jones (1991c). However,
Samson et al. (2000) mentioned that the two types could not always
be sharply deined.
The phenomenon of two-layered, thickened conidial walls, giving
conidia a somewhat zonate appearance, was initially described
www.studiesinmycology.org
in C. coryphae, for which David (1997) introduced the subgenus
Bistratosporium. A re-examination of type material revealed
that the walls of the conidiophores are also often distinctly twolayered, which was not described and illustrated by David (1997).
During the course of morphotaxonomic studies of fungicolous
and foliicolous Cladosporium species several other species have
been re-described, which possess conidia and conidiophores
with two-layered walls, e.g., C. episclerotiale (Heuchert et al.
2005), C. ushuwaiense and C. oreodaphnes (Schubert 2005b).
Since the conidia of these species are much smaller, wall layers
are not as conspicuous as in C. coryphae but nevertheless two
distinct wall layers are visible. In several collections of C. herbarum,
conidiophores and few conidia with thickened, two-layered walls
have also been observed, i.e., conidiophores and conidia with
one- and two-layered walls are often mixed in particular species
or even single collections of a species. Hence, Bistratosporium is
undoubtedly not tenable at subgeneric rank.
The introduction of subgenera based on the three major
species complexes is probably warranted. Schubert et al. (2007b)
and Bensch et al. (2010) resolved the species complexes of C.
herbarum and C. cladosporioides, which form well-supported
subclades. Species of the C. sphaerospermum complex are
well-characterised by having globose or subglobose, pigmented,
almost smooth to verrucose terminal conidia and 0–3-septate,
smooth or verruculose ramoconidia (Ellis 1971, Zalar et al. 2007)
and share a similar ecology since they are often isolated from
extreme ecological environments, but C. sphaerospermum-like
species may not represent a single monophyletic group but belong
to various species complexes within Cladosporium (Zalar et al.
2007). However, inal conclusions about a subgeneric classiication
of Cladosporium are not yet possible. Additional molecular
examinations of a wider range of species are necessary to see if
more species complexes exist in the genus Cladosporium.
Generic concept of the teleomorph, Davidiella
Davidiella Crous & U. Braun, Mycol. Progr. 2: 8. 2003,
emend. in Schubert et al. (2007b).
Type species: Davidiella tassiana (De Not.) Crous & U. Braun,
Mycol. Progr. 2: 8. 2003.
Basionym: Sphaerella tassiana De Not., Sferiacei Italici 1: 87. 1863.
Ascomata pseudothecial, black to red-brown, globose,
inconspicuous and immersed beneath stomata to supericial,
situated on a reduced stroma, with 1(–3) short, periphysate
ostiolar necks; periphysoids frequently growing down into cavity;
wall consisting of 3–6 layers of textura angularis. Asci fasciculate,
short-stalked or not, bitunicate, subsessile, obovoid to broadly
ellipsoid or subcylindrical, straight to slightly curved, 8-spored.
Pseudoparaphyses frequently present in mature ascomata, hyaline,
septate, subcylindrical. Ascospores bi- to multiseriate, hyaline,
obovoid to ellipsoid-fusiform, with irregular luminar inclusions,
mostly thick-walled, straight to slightly curved; frequently becoming
brown and verruculose in asci; at times covered in mucoid sheath.
Cladosporium anamorph usually produced in culture, but not in all
taxa.
Notes: The genus Davidiella (Cladosporiaceae) was recently
introduced for teleomorphs of Cladosporium s. str. (Braun et
al. 2003). The introduction of Davidiella was mainly based on
11
BenSch et al.
phylogenetic studies within Mycosphaerellaceae (Braun et al. 2003),
where it could be demonstrated that “Mycosphaerella” species with
Cladosporium anamorphs formed a sister clade to Mycosphaerella
(Crous et al. 2000, 2001). Braun et al. (2003) transferred ive species
to Davidiella based on prior established anamorph-teleomorph
connections, though no details were provided pertaining to
morphological differences between Davidiella and Mycosphaerella.
Aptroot (2006) transferred several additional species to Davidiella,
and distinguished them from true Mycosphaerella species by the
presence of distinct, irregular cellular inclusions (lumina) in their
ascospores. Furthermore, Schoch et al. (2006, 2009a, b) placed
Davidiella in a separate family [Cladosporiaceae (Nannizi 1934),
which predates Davidiellaceae (2007)] in Capnodiales. During the
course of the studies within the Cladosporium herbarum species
complex (Schubert et al. 2007b), several fresh specimens of
Davidiella spp. were collected or induced in culture, making it
possible to give an emended circumscription of the genus with
additional features to distinguish it from Mycosphaerella. The
induced Davidiella states were all from homothallic species. The
genus presently contains 33 names (www.MycoBank.org), of which
only around ive have acknowledged Cladosporium states.
MORPHOTAXONOMY OF CLADOSPORIUM S. STR.
Morphology in vivo and in vitro
Mycelium
The mycelium of foliicolous as well as saprobic Cladosporium
species in vivo is often internal, but can also be both internal
and external or exclusively external. The hyphae are consistently
septate, mostly branched, smooth, occasionally somewhat roughwalled, and subhyaline, lightly pigmented to dark brown, thinwalled, but sometimes becoming thick-walled with age. Some
species are characterised by having very wide hyphae, e.g., C.
gentianae, while others sometimes possess somewhat lobed
hyphae, e.g., C. grech-delicatae and C. foliorum, but these features
are of little value for the delimitation of species. Particular hyphal
cells are often somewhat swollen and form small to large, loose
aggregations or dense stromata. However, the ability to form
stromata is often variable and not diagnostic.
In vitro, stromata are usually lacking, and the mycelium is
often very variable, ranging from narrow, subhyaline to much
wider, distinctly pigmented hyphae, thin-walled, but sometimes
even with somewhat thickened walls with age. Some species are
able to form hyphal strands or expanded supericial hyphal ropes,
e.g., C. angustisporum, C. australiense, C. perangustum and C.
tenellum while others form dimorphic hyphae as C. antarcticum.
The mycelium in most of the Cladosporium species treated in the
C. cladosporioides complex is more or less Zasmidium (Stenella-)
-like in vitro, being verruculose or verrucose to irregularly roughwalled, an observation not previously documented from the natural
hosts since the fungal hyphae are usually intercellular in host tissue
(Bensch et al. 2010). In general, the features of the hyphae are of
little diagnostic and taxonomic value.
Conidiophores
In vivo the conidiophores in species of the genus Cladosporium
usually arise from internal or external hyphae, from small to large
stromatic hyphal aggregation, but occasionally even from sterile
or immature pseudothecium-like bodies [see Webster & Weber
12
(2007: 485, igs 17, 23), Dugan & Rector (2007: 10, igs 1–4)].
They are mostly cylindrical, subcylindrical or iliform, but further
differentiation is often due to sympodial proliferations causing
geniculations with conidiogenous loci often situated on small lateral
shoulders or terminal to intercalary swellings. Several species
are well-characterised by having mildly to distinctly geniculatesinuous conidiophores, e.g., C. hypophyllum, C. minusculum and
C. varians, but strongly geniculate-sinuous conidiogenous cells are
also known, e.g. in C. sinuosum. Different degrees of intercalary
and apical inlation of the conidiophores are possible, ranging from
subnodulose to nodose. The swellings may be circumferential or
unilateral. Small, unilateral swellings are known in C. tenuissimum.
The term subnodulose is used when lateral shoulders become
slightly increased. Nodulose conidiophores, as in C. allicinum, C.
herbarum, C. herbaroides, C. macrocarpum, C. subinlatum, C.
trillii and C. variabile, possess circumferential swellings around
the stalks, such swellings often being formed in quick succession,
giving conidiophores a somewhat gnarled or knotty appearance.
Nodose conidiophores with distinct, regular, more pronounced
swellings, clearly separated and distant from each other, are
formed in C. colocasiae and C. oxysporum. The process of
conidiogenesis within the latter two species has been described in
detail by McKemy & Morgan-Jones (1991b).
As conidiophores become temporarily determinate, linear apical
growth ceases. The conidiophores swell appreciably at the extreme
apex and a few conidia are formed in close proximity to one another
at the surface of the inlated portion. Following such conidiation,
apical meristematic terminal growth resumes giving rise initially
to a narrow, hypha-like extension above the fertile node. This
grows to varying lengths, depending upon growing conditions. The
extended distal portion usually becomes separated from the node
below by a transverse septum and then ceases growth. Terminal
swellings and conidiation then ensue at the higher level and the
sequence of events is repeated a number of times to give rise to
the characteristic nodose morphology. Whether the conidiogenous
loci are conined to swellings or not, is also an important feature
for the discrimination of species. Within a species the shape of
the conidiophores is usually uniform, but in a few cases, e.g., C.
fusicladiiformis and C. chrysophylli, they are dimorphic: two different
types of conidiophores are formed, which morphologically vary in
their length, width, septation, pigmentation and sometimes in the
thickness of the walls. Much smaller micronematous conidiophores
are often formed in culture beside the common macronematous
conidiophores (Schubert et al. 2007b, Bensch et al. 2010).
Peculiarities of the arrangement of conidiophores in vivo
are often diagnostic in combination with other characteristics.
The number of conidiophores per fascicle is mostly variable, but
general circumscriptions, e.g. “conidiophores solitary or in small
loose groups” or “conidiophores numerous, in dense fascicles”, are
workable and useful. A few species within the genus Cladosporium
form conspicuous fascicles of conidiophores, e.g., C. soldanellae
and C. oreodaphnes. In vitro the conidiophores are almost
consistently formed singly, arising from plagiotropous hyphae, i.e.,
aggregations of conidiophores in fascicles or sporodochia are rarely
formed in culture. Branched conidiophores occur in a number of
species such as C. diaphanum, C. rectangulare, C. sarmentorum,
C. smilacicola and C. sphaerospermum. The ramiication of the
conidiophores (presence, degree, topology) is an additional useful
feature, but can be affected by age and environmental conditions.
Conidiophores branched in vivo are usually also branched in
culture. The length of the conidiophores is usually variable, often
strongly inluenced by external conditions, and must be applied with
the genuS Cladosporium
caution. Some species are well-distinguished by having constantly
short, fasciculate conidiophores under natural conditions, usually
not longer than 60 µm, as in C. lupiniphilum, C. praecox and C.
rutae. However, it has been reported by several authors (e.g., Ellis
1971, Morgan-Jones & McKemy 1990) that conidiophore length
can be extremely variable (sometimes twice as long as under ield
conditions), when incubated in a damp chamber or when grown
in vitro. The width of conidiophores is usually less variable. The
occurrence and number of septa depend on conidiophore length.
Under light microscopy the walls of the conidiophores can either
be recognised as a single wall layer or when distinctly thickened,
two layers can be observed, e.g., in C. apicale and C. ushuwaiense.
The outer wall, which can be ornamented, is often somewhat wider
and paler than the inner wall layer. In combination with other
characteristics the width of the conidiophore walls can be used
as an additional feature to discriminate species. During periods
of unfavourable conditions, in some cases the conidiophores of
Cladosporium can stop growing and the walls become rigid. When
these conditions are over, the conidiophores may resume growth
to produce new conidiogenous cells. The apical conidiophore wall
ruptures through the enteroblastic proliferation of an internal layer
of the wall, visible as discontinuity in pigmentation and in thickness
of the wall. David (1997), who described this phenomenon in detail,
used the term enterogenous, but the term enteroblastic, expanded
and applied by Minter et al. (1982) to mechanisms of budding in
general, covering proliferations of conidiophores, conidiogenous
cells and conidia, should be preferred. Enteroblastic proliferations
are evident in C. orchidiphilum and C. populicola. In several species,
the lumen of the conidiophore cells may be distinctly diminished or
the protoplasm of the conidiophore cells appears to be somewhat
aggregated at the septa (similar to distoseptation), giving septa
and above all walls a somewhat thickened appearance, e.g., in C.
fusicladiiformis. This phenomenon could also be observed in a few
species of the genera Fusicladium and Passalora, and reminds
one of the features of the ascospores in Davidiella which are often
also characterised by having distinct irregular luminar inclusions
(Aptroot 2006, Schubert et al. 2007b).
Conidiogenous cells
The conidiogenous cells are integrated, terminal or intercalary,
or sometimes conidiophores are reduced to conidiogenous cells.
De Vries (1952) recognised two types of conidial heads. The
sympodial growth with regular prolongations of the conidiophores
giving rise to new conidiogenous loci as in C. herbarum was
referred to as the “Cladosporium type” and is common within
the genus. In a few species, such as C. tenuissimum and C.
cladosporioides, there is comparatively little, if any, sympodial
conidiophore growth, no prolongations and thus a limited number
of conidiogenous loci occur. A large diversity of conidia is formed
as a result of the formation of primary, secondary and tertiary
conidia. This second type was called the “Hormodendrum type”.
Recognition of these types and variation among them can be useful
in better deining species concepts and in identiication (McKemy
& Morgan-Jones 1991c). De Vries (1952) considered these
two types to occur commonly in any species of Cladosporium in
culture. However, in some species conidiophores do not proliferate
sympodially, so that the “Hormodendrum type” is a full expression
of the potential of the species concerned (David 1997). David
(1997) considered the distinction between proliferating and nonproliferating conidiophores to be more important than recognised
by de Vries (1952) and introduced a sectional division of subgenus
www.studiesinmycology.org
Cladosporium on the basis of types of conidiophore growth
patterns. Section Hormodendropsis comprises Cladosporium
species with determinate conidiophores and section Cladosporium
is characterised by having indeterminate, sympodially proliferating
conidiophores. Samson et al. (2000) mentioned that these two
types could not always be sharply deined. In culture, Hennebert
& Sutton (1994) recognised the phenomenon that some species
may initially produce “Hormodendrum type” conidial heads but
subsequently proliferate and thus become “Cladosporium type”.
The latter observations could be conirmed during the course of
present monographic studies (Schubert 2005b). Differences in the
proliferation of conidiogenous cells are, indeed, not clearly deined,
and intermediate types occur. Hence, these features should not be
used for the separation of Cladosporium into smaller units.
The structure of the conidiogenous loci and conidial hila is more
or less uniform within the genus Cladosporium; differences noted
between species are only minimal and gradual. This character is
above all signiicant at the generic level as stated above. Roquebert
(1981) carried out the irst detailed SEM examinations of the scar
structure. David (1997) followed the terminology introduced in the
latter paper and published the irst comprehensive circumscription
of the conidiogenous loci and hila. They are protuberant, thickened,
refractive to somewhat darkened and consist of a central convex
dome, which is the slightly bulging half of the original septum,
delimiting the conidium and the conidiogenous cell, after being
cleft, and a raised periclinal rim, where the walls were joined prior
to secession (coronate or “Cladosporium type” of scars according
to Braun et al. 2003). The main source of variation is in the degree
of thickening of the rim, size and protuberance can vary with age.
The size of these structures is often not very variable between
Cladosporium species (mostly 1–2 µm diam) and therefore of
limited value for the discrimination of species.
Conidial secession in Cladosporium is schizolytic (David 1997),
i.e., the basal separating septum splits at the middle layer by cleavage.
However, there are some peculiarities that distinguish this process
and the resulting scar structure from other hyphomycete genera. The
conidiogenous loci are distinctly protuberant and split in the middle,
leaving a conspicuous fringe (raised periclinal rim). Mature conidia
separate at the outer rim, but remain attached at the central dome,
which secedes later. This process results in a conspicuous central
papilla-like structure or dome (David 1997: 15, ig. 2A, Schubert et
al. 2007b: 119, ig. 10B, 123, ig. 15C–F, 131, ig. 24D). David (1997)
classiied this unique scar type to be coronate, but since peculiar and
conined to Cladosporium s. str. it is also appropriate to simply call it
“Cladosporium type” (Braun et al. 2003).
Conidia
All Cladosporium species have the potential to produce conidia
in true acropetal chains. Species with solitary conidia on the host
usually have the capacity to produce conidia in chains in culture.
The formation of the conidia in chains or solitary is a useful
feature to differentiate particular species, but it is not tenable at
the generic level. Conidial chains within the genus Cladosporium
are acropetal, sympodial and often profusely branched. The term
ramoconidia has been used by several authors (e.g., Ellis 1971,
1976, McKemy & Morgan-Jones 1991c, David 1997) for those
conidia at the base of branches having more than one distal scar.
Kirk et al. (2001) provided a deinition of the term ramoconidium,
describing it as a branch of a conidiophore, which secedes and
functions as a conidium, which means that it represents a detached
conidiogenous cell. In Cladosporium, they are characterised by
13
BenSch et al.
having a truncate or slightly convex, unthickened base, without any
dome or raised rim, which could be conirmed by light and scanning
electron microscopy (see Schubert 2005b: pl. 24, ig. E). Schubert
(2005b) and Heuchert et al. (2005) used for these “true” ramoconidia
the term “ramoconidia s. str.”, and classiied branched “normal”
conidia occurring at the base or within the chains, characterised
by having a basal coronate hilum and more than one distal scar,
as ramoconidia s. lat. Due to the structure of the conidial base,
both types are easily separable. However, Schubert et al. (2007b)
redeined the different conidial types and followed Kirk et al. (2001)
in conining the term “ramoconidium” to detached conidiogenous
cells or short, fertile, terminal branches, and reclassiied branched
conidia (ramoconidia s. lat., according to Heuchert et al. 2005 and
Schubert 2005b) as secondary ramoconidia. The presence of
ramoconidia is a feature of limited value for the characterisation
of Cladosporium species, since these structures are often rarely
formed or even lacking. If ramoconidia are present, a combination
of length, width and septation of these structures may be useful for
the discrimination of particular groups or distinct species.
The conidial shape is highly variable and only little differentiated
between the species examined, ranging from subglobose, ovoid,
ellipsoid, fusiform, limoniform to subcylindrical or cylindrical. The
conidial length and the degree of septation are also often variable
and depend on external conditions so that these characters can
only be used in combination with other taxonomic features. The
width of the conidia is, however, less variable and rather suitable
for the delimitation of allied taxa.
The shape and size of conidia within long acropetal conidial
chains of Cladosporium species is often strongly variable, differing
from base to apex. Ramoconidia, if present, are often followed by
septate secondary ramoconidia, smaller intercalary conidia and
very small, usually aseptate terminal conidia. In order to enable
more differentiated, precise descriptions of conidia in Cladosporium
species, Schubert et al. (2007b) proposed a redeined conidium
terminology (Fig. 3), applying the terms: ramoconidium, secondary
ramoconidium, intercalary conidium and small terminal conidium.
Peculiarities of conidial surface ornamentation provide useful
criteria for the separation of species, but must be judged with
caution. A general grouping, e.g., “conidia smooth or almost so”, or
“conidia verruculose or verrucose” is, however, workable. Species
with verruculose or conspicuously verrucose-echinulate conidia are
easily distinguishable from those with conidia that are smooth or
almost so. However, in several species some variation in surface
ornamentation can occur, e.g., in C. herbarum. Conidia of the latter
species are mainly verruculose, but sometimes a few smooth
conidia may be intermixed. Older conidia of species with usually
smooth conidia sometimes become somewhat rough-walled, e.g.,
in C. fusicladiiformis, C. lineolatum and in C. oncobae. Surface
ornamentation of conidia in the C. cladosporioides complex is
quite variable ranging from smooth or almost so to irregularly
verruculose-rugose, verrucose or rough-walled in some species.
This is comparable with the C. sphaerospermum complex in which
species with both smooth-walled as well as ornamented conidia are
included (Zalar et al. 2007), whereas all species in the C. herbarum
complex possess ornamented conidia with the ornamentation
ranging from minutely verruculose to verrucose, echinulate or spiny
(Fig. 4). The most prominent surfaces within the C. cladosporioides
complex are formed by C. acalyphae, C. exasperatum and C.
verrucocladosporioides. Under SEM the surface of their irregularly
verruculose-rugulose conidia show irregularly reticulate structures
or embossed stripes. This phenomenon was also described and
illustrated for powdery mildew anamorphs (Cook et al. 1997, Braun
14
Fig. 3. Overview showing the different types of cladosporioid conidia. Cladosporium
conidiophore with ramoconidia, secondary ramoconidia, intercalary conidia, and
small, terminal conidia. Scale bar = 10 µm. K. Bensch del.
et al. 2002). Cladosporium cladosporioides usually forms smooth
conidia (LM) but under SEM such wrinkled structures or embossed
stripes are also visible. They are not as prominent as in C. acalyphae
or C. exasperatum and therefore not to be seen when using light
microscopy and seem to occur more commonly in older conidia.
Several species are characterised by irregular ornamentation on
the small terminal and intercalary conidia, whereas secondary
ramoconidia are smooth or almost so, as in C. inversicolor, C.
acalyphae and C. rectoides. Combined with additional taxonomic
features, this characteristic can be used for species delimitation.
Since there are only few taxonomically relevant features
within the genus Cladosporium and in hyphomycetes in general,
attention has been paid to characteristic cell structures, hitherto
barely used for taxonomic purposes. In several species, as in
C. arthrinioides, C. heliotropii and in C. minusculum, the conidial
cells are characterised by having a reduced, conspicuous lumen
the genuS Cladosporium
Aculeate
Spinulose
Digitate
Muricate
Granulate
Colliculate
Pustulate
Pedicellate
Fig. 4. Terms used to describe conidium wall ornamentation under the cryo-electron
microscope. Adapted from David (1997).
(Schubert 2005b: igs 8, 33, 34, 45), reminiscent of the irregular
luminar inclusions in ascospores of Davidiella (Aptroot 2006). This
structure has been observed both in type material of C. heliotropii,
described from Sweden, and in additional collections of this species
collected in Alaska so that it may be used as distinctive feature. In
some cases, the lumen of the conidial and also conidiophore cells
often appears to be distinct, clearly separated from the inner wall
(e.g., in C. syringicola and C. populicola). Peculiarities of the cell
structure are, however, little examined and probably of limited value
at species level.
as geographical distribution are also taken into consideration.
All species are comprehensively compared with morphologically
similar species as well as species which occur on host plants of the
same plant family or the same substrate.
The following features proved to be diagnostic for the differentiation
at species rank:
• Shape of the conidiophores (geniculate-sinuous, nodulose,
location of the conidiogenous loci, dimorphism).
• Ramiication of the conidiophores (presence, topology,
degree).
• Width of the conidiophores.
• Formation of conidia (solitary or catenate, unbranched or
branched chains).
• Conidial surface ornamentation.
• Symptoms, lesions, host speciity in biotrophic species.
The following features are only diagnostic in combination with other
features:
• Mycelium (internal, external, both internal and external in
biotrophic species).
• Arrangement of the conidiophores in biotrophic species
(solitary, fasciculate).
• Length, septation and thickness of the conidiophore wall.
• Conidiogenous cells (terminal, intercalar, conidiophores
reduced to conidiogenous cells; proliferation sympodial,
enteroblastic; number and width of conidiogenous loci).
• Ramoconidia (presence, length, width, septation).
• Conidia (length, width, septation, shape, cell structure).
The following features are either more or less uniform or highly
variable and thus less appropriate for the discrimination of species:
• Structure of the mycelium.
• Formation of stromata in vivo.
• Formation of conidiophores (arising from stromata or hyphae).
• Structure of the conidiogenous loci and hila (generic feature).
• Degree of pigmentation of conidiophores and conidia.
Phylogenetic features: Molecular sequence analysis is a helpful tool
to differentiate closely allied, morphologically similar species, above
all with regard to saprobic taxa. Less closely allied species with
obvious morphological differences, e.g. species with smooth versus
verruculose or echinulate conidia, are genetically usually clearly distinct
and usually form clearly separate clusters, even in phylograms based
on a single locus (e.g. rDNS ITS) (Braun et al. 2003). However, within
complexes of morphologically similar, closely allied taxa, ITS data are
often not suficient to discriminate species, i.e., the resolution is often
too poor, resulting in trees with polytomous structures. During the
course of detailed genetic as well as morphological examinations of
the Cladosporium herbarum complex, it could be demonstrated that a
multilocus DNA sequence approach, based on ITS, actin, calmodulin,
translation elongation factor 1-α, and histone H3, led to a much better
resolution and distinction of closely allied taxa (Schubert et al. 2007b).
In Bensch et al. (2010) a combination of three loci was used to deine
species entities within the C. cladosporioides complex (Fig. 5).
SPECIES CONCEPT
BIOLOGY, ECOLOGY AND DISTRIBUTION
The circumscriptions and delimitations of the species concerned are
mainly based on quantitative as well as qualitative morphological
features in vivo and in vitro and on molecular data if cultures were
available. Host range and specialisation of biotrophic taxa as well
www.studiesinmycology.org
Cladosporium species have an extremely wide ecological range,
occurring on all kinds of substrates, and on a wide range of hosts,
either biotrophically or on dead or senescing tissue. In contrast
15
BenSch et al.
Cercospora beticola CBS 116456
Cladosporium salinae CBS 119413
Cladosporium aphidis CPC 13204
100
Cladosporium langeronii CBS 189.54
Cladosporium psychrotolerans CBS 119412
C. sphaeroCladosporium halotolerans CBS 119416
100
spermum
Cladosporium
dominicanum
CBS
119415
57
2x
Cladosporium fusiforme CBS 119414
99
complex
Cladosporium velox CBS 119417
Cladosporium sphaerospermum CBS 193.54
57
79
Cladosporium basiinflatum CBS 822.84
56
Cladosporium ramotenellum CBS 121628
100
Cladosporium arthropodii CBS 124043
Cladosporium spinulosum CBS 119907
Cladosporium tenellum CBS 121634
86
Cladosporium allicinum CBS 121624
99
55
Cladosporium subtilissimum CBS 113754
Cladosporium subinflatum CBS 121630
Cladosporium ossifragi CBS 842.91
100 Cladosporium soldanellae CPC 13153
C. herbarum
Cladosporium antarcticum CBS 690.92
complex
Cladosporium sinuosum CBS 121629
51
Cladosporium
pseudiridis
CBS
116463
20 changes
91
67
Cladosporium allii CBS 101.81
Cladosporium echinulatum CBS 123191
Cladosporium iridis CBS 138.40
Cladosporium phlei CBS 358.69
52
Cladosporium variabile CBS 121635
60
Cladosporium herbarum CBS 121621
92
Cladosporium herbaroides CBS 121626
65
Cladosporium macrocarpum CBS 121623
Cladosporium grevilleae CBS 114271
Cladosporium perangustum CBS 125996
67
Cladosporium flabelliforme CBS 126345
Cladosporium exasperatum CBS 125986
69
Cladosporium scabrellum CBS 126358
100
Cladosporium chalastosporoides CBS 125985
Cladosporium hillianum CBS 125988
Cladosporium exile CBS 125987
Cladosporium paracladosporioides CBS 171.54
Cladosporium varians CBS 126362
84
Cladosporium globisporum CBS 812.96
Cladosporium iranicum CBS 126346
Cladosporium phyllophilum CBS 125992
65
100 Cladosporium licheniphilum CBS 125990
96 Cladosporium phyllactiniicola CBS 126355
Cladosporium asperulatum CBS 126340
Cladosporium myrtacearum CBS 126350
100 Cladosporium pini-ponderosae CBS 124456
Cladosporium chubutense CBS 124457
C. cladosporioides
99 Cladosporium colombiae CBS 274.80B
complex
Cladosporium lycoperdinum CBS 574.78C
99
Cladosporium delicatulum CBS 126344
81
87 51 Cladosporium silenes CBS 109082
Cladosporium acalyphae CBS 125982
64
Cladosporium inversicolor CBS 401.80
Cladosporium
rectoides CBS 125994
82
81
Cladosporium xylophilum CBS 125997
Cladosporium cladosporioides CBS 112388
84
Cladosporium angustisporum CBS 125983
Cladosporium cucumerinum CBS 171.52
84
Cladosporium subuliforme CBS 126500
Cladosporium colocasiae CBS 386.64
Cladosporium tenuissimum CBS 125995
100
Cladosporium funiculosum CBS 122129
62
Cladosporium pseudocladosporioides CBS 125993
66
Cladosporium gamsianum CBS 125989
Cladosporium verrucocladosporioides CBS 126363
Cladosporium australiense CBS 125984
Cladosporium phaenocomae CBS 128769
100
Fig. 5. The irst of 12 equally most parsimonious trees obtained from a heuristic search with 100 random taxon additions of the combined ITS, EF-1α and ACT sequence
alignment. The scale bar shows 20 changes, and bootstrap support values from 1 000 replicates are shown at the nodes. Thickened lines indicate branches present in the strict
consensus tree and the major species complexes are indicated in coloured blocks. The tree was rooted to sequences of Cercospora beticola strain CBS 116456 (GenBank
accession numbers AY840527, AY840494, AY840458, respectively).
16
the genuS Cladosporium
to previous assumptions, only a limited number of species are
plurivorous, widely distributed saprobic species, as e.g. C. herbarum,
C. cladosporioides and C. oxysporum, which do not appear to have
any strong environmental preferences. On the other hand, there
are some saprobic species inhabiting particular, limited ecological
niches. Schubert et al. (2007b) described, for instance, several new
species of the C. herbarum complex from hypersaline environments,
and Zalar et al. (2007) found various new halophilic and halotolerant
C. sphaerospermum-like species. Some Cladosporium species are
fungicolous using other fungi as substrate (Heuchert et al. 2005), but
numerous species are biotrophic, often host-speciic, causing typical
leaf spots, discolorations, necrosis or shot hole symptoms on living
or senescing leaves. Some of them may develop almost without any
visible symptoms (e.g., C. obtectum) or attack stems (e.g., C. grechdelicatae). For these biologically specialised taxa the host range is an
important feature. They usually follow the distribution of their hosts.
Cultivated hosts are usually colonised wherever grown. The host
ranges are usually more or less conined, not exceeding the limits
of a single host family, mostly even narrower, often only covering
few species of a single genus. However, there are some exceptions,
e.g., C. allii, C. allii-cepae and C. victorialis on various species of
Allium. While C. allii-cepae is known to be conined to Allium cepa
(Kirk & Crompton 1984), C. allii is reported to have a wider host
range. David (1997) concluded that this may indicate that C. alliicepae has evolved with its host to become distinct, whereas C. allii
is the original form, known to occur on non-cultivated members of
the genus Allium. Cladosporium victorialis, recorded from Korea and
Russia, is morphologically quite distinct from the latter two species,
so it could be assumed that Allium has been colonised twice.
A surprising inding from the molecular studies in the three
species complexes recently studied (Schubert et al. 2007b, Zalar
et al. 2007, Bensch et al. 2010) was the huge diversity in species
and genotypes that exist in nature, be it in indoor environments,
on fruit surfaces, or in extreme ecological niches such as salterns,
etc. Several isolates from a single substrate in a single location,
e.g., chasmothecia of Phyllactinia guttata on leaves of an individual
plant, Corylus avellana (Dugan & Glawe 2006; table 1, Bensch
et al. 2010) or grapes in the USA (Schubert et al. 2007b) can be
colonised by various genotypes representing several completely
different species. The phenomenon of co-occurrence of many
species on the same lesions on a single host in Mycosphaerella
and Teratosphaeria leaf disease complexes has been frequently
described and discussed (Crous 1998, Crous et al. 2004b, 2007a,
2008a, b, 2009e, Crous & Groenewald 2005, Burgess et al. 2007,
Arzanlou et al. 2008, Cheewangkoon et al. 2008). Therefore, it is
not surprising that co-occurring genotypes or species also exist
in the related genus Cladosporium (also see Wirsel et al. 2002),
suggesting that special care needs to be taken during the isolation
and culturing of these taxa.
MATERIAL AND METHODS
Microscopic examinations were carried out based on collections
from numerous herbaria and fresh specimens. The collections
examined are deposited at numerous herbaria: B, BPI, BRIP,
CUP, DAOM, CBS, FH, HAL, HBG, IACM, ILL, IMI, INIFAT, K, KR,
LBLM, LE, LEP, LPS, M, MA, NY, NYS, PAD, PC, PDD, PH, PPMH,
PRM, S, SIENA, VPRI, W, WIS, etc. (abbreviations according to
Holmgren et al. 1990). Details on other methods, like scanning
electron microscopy and molecular sequence analyses are outlined
www.studiesinmycology.org
in the papers concerned (see Braun et al. 2003, Heuchert et al.
2005, Schubert 2005b, Crous et al. 2007a, Schubert et al. 2007b,
Bensch et al. 2010).
Isolates and herbarium specimens
Microscopic examinations were carried out based on collections
from numerous herbaria, some fresh specimens and hundreds of
isolates. The collections examined are deposited at the following
herbaria: B, BPI, BRIP, C, CBS, CUP, DAOM, DAR, FH, HAL, HBG,
IACM, ILL, IMI, INIFAT, K, KR, LBLM, LE, LEP, LPS, M, MA, NY,
NYS, PAD, PC, PDD, PH, PPMH, PRM, S, SIENA, VPRI, W, WIS
(abbreviations according to Holmgren et al. 1990). Isolates included
in this or previous studies were obtained from the culture collection
of the Centraalbureau voor Schimmelcultures (CBS), Utrecht, the
Netherlands, or were freshly isolated from a range of different
substrates. Single-conidial and ascospore isolates were obtained
using the techniques as explained in Crous (1998) for species of
Mycosphaerella and its anamorphs. Isolates were inoculated onto
2 % potato-dextrose agar (PDA), synthetic nutrient-poor agar
(SNA), 2 % malt extract agar (MEA) and oatmeal agar (OA) (Crous
et al. 2009d), and incubated under continuous near-ultraviolet
light at 25 °C to promote sporulation. All cultures obtained in this
study are maintained in the culture collection of the CBS (Table
1). Nomenclatural novelties and descriptions were deposited in
MycoBank (www.MycoBank.org; Crous et al. 2004a).
DNA isolation, ampliication and sequence analysis
Genomic DNA was extracted from mycelia of fungal colonies
cultivated on MEA using the UltraCleanTM Microbial DNA Isolation
Kit (Mo Bio Laboratories, Inc., Solana Beach, CA, USA) according
to the manufacturer’s instructions. The primers V9G (de Hoog &
Gerrits van den Ende 1998) and LR5 (Vilgalys & Hester 1990) were
used to amplify part of the nuclear rDNA operon (ITS) spanning
the 3’ end of the 18S nrRNA gene, the irst internal transcribed
spacer, the 5.8S nrRNA gene, the second ITS region and the irst
approximately 900 bp of the 5’ end of the 28S nrRNA gene (LSU).
The primers ITS4 (White et al. 1990) and LR0R (Rehner & Samuels
1994) or LSU1Fd (Crous et al. 2009b) were used as internal
sequence primers to ensure good quality sequences over the
entire length of the amplicon. To obtain resolution at species level
for Cladosporium, the ITS region was supplemented with partial
gene sequences of the translation elongation factor 1-α gene (EF1α) using the primers EF1-728F and EF1-986R (Carbone & Kohn
1999) or EF-2 (O’Donnell et al. 1998) and the actin gene (ACT)
using the primers ACT-512F and ACT-783R (Carbone & Kohn
1999). The PCR ampliications were performed on a GeneAmp PCR
System 9700 (Applied Biosystems, Foster City, CA, USA) in a total
volume of 12.5 µL solution containing 10–20 ng of template DNA,
1 × PCR buffer, 2.0 mM MgCl2, 2.5 pmol of each primer, 20 µM
of each dNTP and 0.5 U BioTaq DNA polymerase (Bioline GmbH,
Luckenwalde, Germany). For TEF, 0.7 µL of water with a similar
volume of DMSO (Dimethyl sulfoxide) was found to increase the
quality of the ampliication reaction. PCR ampliication conditions
were set as follows: an initial denaturation temperature of 94 °C
for 5 min, followed by 40 cycles of denaturation temperature of
94 °C for 45 s, primer annealing at 48 °C (52 °C for TEF and ACT)
for 30 s, primer extension at 72 °C for 90 s and a inal extension
step at 72 °C for 6 min. The resulting fragments were sequenced
using the PCR primers (and internal primers for the combined
17
Species
Culture accession number(s)1
Status of strain
GenBank accession numbers2
ITS
TEF
ACT
Reference
HIS
CAL
LSU
TUB
SSU
C. acalyphae
CBS 125982; CPC 11625
Ex-type from holotype
HM147994
HM148235
HM148481
—
—
—
—
—
Bensch et al. (2010)
C. allicinum
CBS 121624; CPC 12211
Ex-type from neotype
EF679350
EF679425
EF679502
EF679655
EF679578
—
—
—
Schubert et al. (2007b)
C. allii
CBS 101.81; ATCC 200948; PD 80/165
Reference strain
JN906977
JN906983
JN906996
—
—
—
—
—
Present study
C. angustisporum
CBS 125983; CPC 12437
Ex-type from holotype
HM147995
HM148236
HM148482
—
—
—
—
—
Bensch et al. (2010)
C. antarcticum
CBS 690.92
Ex-type from holotype
EF679334
EF679405
EF679484
EF679636
EF679560
EF679334
—
—
Schubert et al. (2007b)
C. aphidis
CPC 13204
Ex-type from epitype
JN906978
JN906984
JN906997
—
—
—
—
—
Present study
C. arthropodii
CBS 124043; CPC 16160
Ex-epitype from epitype
JN906979
JN906985
JN906998
—
—
—
—
—
Present study
C. asperulatum
CBS 126340; CPC 14040
Ex-type from holotype
HM147998
HM148239
HM148485
—
—
—
—
—
Bensch et al. (2010)
C. australiense
CBS 125984; CPC 13226
Ex-type from holotype
HM147999
HM148240
HM148486
—
—
—
—
—
Bensch et al. (2010)
C. basiinlatum
CBS 822.84
Ex-type from holotype
HM148000
HM148241
HM148487
—
—
—
—
—
Bensch et al. (2010)
C. chalastosporoides
CBS 125985; CPC 13864
Ex-type from holotype
HM148001
HM148242
HM148488
—
—
—
—
—
Bensch et al. (2010)
C. chubutense
CBS 124457; CPC 13979; CIEFAP 321
Ex-type from holotype
FJ936158
FJ936161
FJ936165
—
—
—
—
—
Schubert et al. (2009)
C. cladosporioides
CBS 112388
Ex-type from neotype
HM148003
HM148244
HM148490
—
—
—
—
—
Bensch et al. (2010)
C. colocasiae
CBS 386.64; ATCC 200944; MUCL 10084
Ex-type from holotype
HM148067
HM148310
HM148555
—
—
AY342121
—
—
Bensch et al. (2010)
C. colombiae
CBS 274.80B
Ex-type from holotype
FJ936159
FJ936163
FJ936166
—
—
—
—
—
Schubert et al. (2009)
C. cucumerinum
CBS 171.52; MUCL 10092
Ex-type from epitype
HM148072
HM148316
HM148561
—
—
—
—
—
Bensch et al. (2010)
C. delicatulum
CBS 126344; CPC 11389
Reference strain
HM148081
HM148325
HM148570
—
—
—
—
—
Bensch et al. (2010)
C. dominicanum
CBS 119415; EXF-732; dH 16386
Ex-type from holotype
DQ780353
JN906986
EF101368
—
—
—
EF101415
—
Zalar et al. (2007)
C. echinulatum
CBS 123191; CPC 15386
Reference strain
JN906980
JN906987
JN906999
—
—
—
—
—
Present study
C. exasperatum
CBS 125986; CPC 14638
Ex-type from holotype
HM148090
HM148334
HM148579
—
—
—
—
—
Bensch et al. (2010)
C. exile
CBS 125987; CPC 11828
Ex-type from holotype
HM148091
HM148335
HM148580
—
—
—
—
—
Bensch et al. (2010)
C. labelliforme
CBS 126345; CPC 14523
Ex-type from holotype
HM148092
HM148336
HM148581
—
—
—
—
—
Bensch et al. (2010)
C. funiculosum
CBS 122129; ATCC 38010; IFO 6537; JCM
10683
Ex-type from type
HM148094
HM148338
HM148583
—
—
AY342129
—
—
Bensch et al. (2010)
C. fusiforme
CBS 119414; EXF-449; dH 16387
Ex-type from holotype
DQ780388
JN906988
EF101372
—
—
—
EF101446
DQ780935
Zalar et al. (2007)
C. gamsianum
CBS 125989; CPC 11807
Ex-type from holotype
HM148095
HM148339
HM148584
—
—
—
—
—
Bensch et al. (2010)
C. globisporum
CBS 812.96
Ex-type from holotype
HM148096
HM148340
HM148585
—
—
—
—
—
Bensch et al. (2010)
C. grevilleae
CBS 114271; CPC 2913
Ex-type from holotype
JF770450
JF770472
JF770473
—
—
JF770462
—
—
Crous et al. (2011b)
C. halotolerans
CBS 119416; EXF-572
Ex-type from holotype
DQ780364
JN906989
EF101397
—
—
—
EF101424
DQ780929
Zalar et al. (2007)
C. herbaroides
CBS 121626; CPC 12052; EXF-1733
Ex-type from holotype
EF679357
EF679432
EF679509
EF679662
EF679585
—
—
—
Schubert et al. (2007b)
C. herbarum
CBS 121621; CPC 12177
Ex-epitype from epitype
EF679363
EF679440
EF679516
EF679670
EF679592
—
—
—
Schubert et al. (2007b)
C. hillianum
CBS 125988; CPC 15459
Ex-type from holotype
HM148097
HM148341
HM148586
—
—
—
—
—
Bensch et al. (2010)
C. inversicolor
CBS 401.80; ATCC 200941
Ex-type from holotype
HM148101
HM148345
HM148590
—
—
AY342114
—
—
Bensch et al. (2010)
BenSch et al.
18
Table 1. GenBank and culture collection accession numbers of Cladosporium species treated in the species phylogeny.
Table 1. (Continued).
www.studiesinmycology.org
Species
Culture accession number(s)1
Status of strain
GenBank accession numbers2
ITS
TEF
ACT
HIS
CAL
LSU
TUB
SSU
C. iranicum
CBS 126346; CPC 11554
Ex-type from holotype
HM148110
HM148354
HM148599
—
—
—
—
—
Reference
Bensch et al. (2010)
C. iridis
CBS 138.40
Ex-epitype from epitype
EF679370
EF679447
EF679523
EF679677
EF679599
DQ008148
—
EU167591
Schubert et al. (2007b)
C. langeronii
CBS 189.54
Ex-type from neotype
DQ780379
JN906990
EF101357
—
—
—
EF101435
DQ780932
Zalar et al. (2007)
C. licheniphilum
CBS 125990; CPC 13224
Ex-type from epitype
HM148111
HM148355
HM148600
—
—
—
—
—
Bensch et al. (2010)
C. lycoperdinum
CBS 574.78C; VKM F-2759
Reference strain
HM148115
HM148359
HM148604
—
—
—
—
—
Bensch et al. (2010)
C. macrocarpum
CBS 121623; CPC 12755
Ex-neotype from neotype
EF679375
EF679453
EF679529
EF679684
EF679606
—
—
—
Schubert et al. (2007b)
C. myrtacearum
CBS 126350; CPC 14567
Ex-type from epitype
HM148117
HM148361
HM148606
—
—
—
—
—
Bensch et al. (2010)
C. ossifragi
CBS 842.91
Ex-epitype from epitype
C. paracladosporioides
CBS 171.54; ATCC 11278, 200943; IFO Ex-type from holotype
6369; IMI 049626; MUCL 917; NCTC 4097
EF679381
EF679459
EF679535
EF679689
EF679611
EF679381
—
—
Schubert et al. (2007b)
HM148120
HM148364
HM148609
—
—
—
—
—
Bensch et al. (2010)
C. perangustum
CBS 125996; CPC 13815
Ex-type from holotype
HM148121
HM148365
HM148610
—
—
—
—
—
Bensch et al. (2010)
C. phaeocomae
CBS 128769; CPC 18223
Ex-type from holotype
JF499837
JF499875
JF499881
—
—
JF499857
—
—
Crous & Groenewald JZ (2011)
C. phlei
CBS 358.69
Ex-epitype from epitype
JN906981
JN906991
JN907000
—
—
—
—
—
Present study
C. phyllactiniicola
CBS 126355; CPC 11830
Ex-type from holotype
HM148153
HM148397
HM148642
—
—
—
—
—
Bensch et al. (2010)
C. phyllophilum
CBS 125992; CPC 11333
Ex-type from epitype
HM148154
HM148398
HM148643
—
—
—
—
—
Bensch et al. (2010)
C. pini-ponderosae
CBS 124456; CPC 13980; CIEFAP 322
Ex-type from holotype
FJ936160
FJ936164
FJ936167
—
—
—
—
—
Schubert et al. (2009)
C. pseudiridis
CBS 116463; LYN 1065; ICMP 15579
Ex-type from holotype
EF679383
EF679461
EF679537
EF679691
EF679613
—
—
—
Schubert et al. (2007b)
C. pseudocladosporioides
CBS 125993; CPC 14189
Ex-type from holotype
HM148158
HM148402
HM148647
—
—
—
—
—
Bensch et al. (2010)
C. psychrotolerans
CBS 119412; EXF-391; dH 16390
Ex-type from holotype
DQ780386
JN906992
EF101365
—
—
—
EF101442
—
Zalar et al. (2007)
C. ramotenellum
CBS 121628; CPC 12043; EXF-454
Ex-type from holotype
EF679384
EF679462
EF679538
EF679692
EF679614
—
—
—
Schubert et al. (2007b)
C. rectoides
CBS 125994; CPC 11624
Ex-type from holotype
HM148193
HM148438
HM148683
—
—
—
—
—
Bensch et al. (2010)
C. salinae
CBS 119413; EXF-335; dH 16389
Ex-type from holotype
DQ780374
JN906993
EF101390
—
—
—
EF101405
DQ780931
Zalar et al. (2007)
C. scabrellum
CBS 126358; CPC 14976; HJS 1031
Ex-type from holotype
HM148195
HM148440
HM148685
—
—
—
—
—
Bensch et al. (2010)
C. silenes
CBS 109082
Ex-type from holotype
EF679354
EF679429
EF679506
EF679659
EF679582
JF770463
—
—
Crous et al. (2011b)
C. sinuosum
CBS 121629; CPC 11839; ICMP 15819
Ex-type from holotype
EF679386
EF679464
EF679540
EF679694
EF679616
—
—
—
Schubert et al. (2007b)
C. soldanellae
CPC 13153
Ex-neotype from neotype
JN906982
JN906994
JN907001
—
—
—
—
—
Present study
CBS 193.54; ATCC 11289; IMI 49637
Ex-neotype from neotype
DQ780343
EU570261
EU570269
—
—
AY342104
EF101406
DQ780925
Zalar et al. (2007)
CBS 119907; CPC 12040; EXF-334
Ex-type from holotype
EF679388
EF679466
EF679542
EF679696
EF679618
—
EF101450
—
Schubert et al. (2007b)
C. subinlatum
CBS 121630; CPC 12041; EXF-343
Ex-type from holotype
EF679389
EF679467
EF679543
EF679697
EF679619
—
EF101448
—
Schubert et al. (2007b)
C. subtilissimum
CBS 113754
Ex-type from holotype
EF679397
EF679475
EF679551
EF679705
EF679627
—
—
—
Schubert et al. (2007b)
C. subuliforme
CBS 126500; CPC 13735
Ex-type from holotype
HM148196
HM148441
HM148686
—
—
—
—
—
Bensch et al. (2010)
C. tenellum
CBS 121634; CPC 12053; EXF-1735
Ex-type from holotype
EF679401
EF679479
EF679555
EF679709
EF679631
—
—
—
Schubert et al. (2007b)
the genuS Cladosporium
19
C. sphaerospermum
C. spinulosum
2
ITS: internal transcribed spacer regions with 5.8S nrRNA gene, TEF: partial translation elongation factor 1-alpha gene, ACT: partial actin gene, HIS: partial histone H3 gene, CAL: partial calmodulin gene, LSU: partial 28S nrRNA gene, TUB: partial betatubulin gene, SSU: partial 18S nrRNA gene.
Bensch et al. (2010)
—
—
—
—
—
HM148721
HM148476
HM148230
Ex-type from holotype
CBS 125997; CPC 12403
ATCC: American Type Culture Collection, Virginia, U.S.A.; CBS: CBS-KNAW Fungal Biodiversity Centre, Utrecht, The Netherlands; CIEFAP: Centro de Investigación y Extensión Forestal Andino Patagónico, Argentina; CPC: Culture collection of Pedro
Crous, housed at CBS; dH: Culture collection of Sybren de Hoog, housed at CBS; EXF: Culture Collection of Extremophilic Fungi, Biotechnical Faculty, Ljubljana, Slovenia; HJS: Personal culture collection of Hans-Josef Schroers, Slovenia; ICMP:
International Collection of Micro-organisms from Plants, Landcare Research, Private Bag 92170, Auckland, New Zealand; IFO: Institute for Fermentation, Osaka, Japan; JCM: Japan Collection of Microorganisms, RIKEN BioResource Center, Saitama,
Japan; MUCL: Mycotheque de l’Universite catholique de Louvain, Laboratoire de Mycologie Systematique et Appliquee, Universite catholique de Louvain, Louvain-la-Neuve, Belgium; NCTC: National Collection of Type Cultures, PHLS Central Public Health
Laboratory, London, U.K.; PD: Plant Protection Service, nVWA, Division Plant, Wageningen, The Netherlands; VKM: All-Russian Collection of Microorganisms, Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences,
Moscow region, Russian Federation.
C. xylophilum
1
Zalar et al. (2007)
Bensch et al. (2010)
—
DQ780937
EF101456
—
—
—
—
—
—
—
EF101388
JN906995
HM148472
DQ780361
Ex-type from holotype
Ex-type from holotype
CBS 119417; CPC 11224; dH 16388
CBS 126363; CPC 12300
C. velox
C. verrucocladosporioides
HM148226
HM148717
Bensch et al. (2010)
Schubert et al. (2007b)
—
—
—
—
—
—
—
EF679632
EF679710
EF679556
HM148715
EF679480
HM148470
EF679402
HM148224
Ex-epitype from epitype
Ex-type from holotype
CBS 121635; CPC 12751
CBS 126362; CPC 13658
C. variabile
C. varians
—
—
SSU
TUB
—
—
LSU
CAL
—
—
HIS
ACT
TEF
HM148442
ITS
HM148197
Ex-type from epitype
CBS 125995; CPC 14253
C. tenuissimum
GenBank accession numbers2
Status of strain
Culture accession number(s)1
Species
Table 1. (Continued).
20
HM148687
Reference
Bensch et al. (2010)
BenSch et al.
ITS + LSU fragment) together with a BigDye Terminator Cycle
Sequencing Kit v. 3.1 (Applied Biosystems, Foster City, CA) and
analysed on a 3730xl DNA Sequencer (Perkin-Elmer, Norwalk, CN,
USA). Consensus sequences were computed using SeqMan v. 9
from the Lasergene package (DNA-STAR, Madison, WI).
The generated sequences were compared with other fungal
DNA sequences from NCBI’s GenBank sequence database using
a blast search; sequences with high similarity were added to the
alignments. The LSU alignment is primarily based on the alignment
of Crous et al. (2007c) and the combined alignment for the species
tree was compiled using the published sequences linked to
earlier publications of the respective species (see Table 1). The
sequences were aligned using the online version of MAFFT (mafft.
cbrc.jp/alignment/server/index.html; Katoh et al. 2002), followed
by manual correction by eye using Sequence Alignment Editor v.
2.0a11 (Rambaut 2002). The phylogenetic analyses of the aligned
sequence data were performed using PAUP (Phylogenetic Analysis
Using Parsimony) v. 4.0b10 (Swofford 2003) and consisted of
neighbour-joining analyses with the uncorrected (‘p’), the Kimura
2-parameter and the HKY85 substitution models. Alignment gaps
were treated as missing data and all characters were unordered and
of equal weight. Any ties were broken randomly when encountered.
For parsimony analyses, alignment gaps were treated as a ifth
character state and all characters were unordered and of equal
weight. Maximum parsimony analysis was performed using the
heuristic search option with 100 random simple taxa additions and
tree bisection and reconstruction (TBR) as the branch-swapping
algorithm. Branches of zero length were collapsed and all multiple,
equally parsimonious trees were saved. The robustness of the trees
obtained was evaluated by 1 000 bootstrap replications (Hillis &
Bull 1993). Tree length (TL), consistency index (CI), retention index
(RI) and rescaled consistency index (RC) were calculated and the
resulting trees were printed with Geneious v. 5.5.4 (Drummond
et al. 2011) and layout was done in Adobe Illustrator CS v. 5.1.
New sequences were lodged in GenBank and the alignments and
phylogenetic trees in TreeBASE (www. treebase.org).
Morphology
Light microscopy (LM)
For herbarium specimens a stereomicroscope was used to select
colonised portions of samples, to excise colonies and mount
them in water on a slide. Staining was usually not necessary,
since Cladosporium species are pigmented. To avoid drying of
the preparations, permanent slides were prepared by sealing the
cover-glasses with Canada balm (SERVA, Heidelberg) and by
putting them into a desiccator for 24 h. Microscopic observations
were made with oil immersion (1000×). Where possible, 30
conidiophores, conidia and other structures were measured in
each collection, and a representative range was depicted. Some
collections were very poor, so that only a smaller number of these
structures could be measured. Drawings were done free hand.
Digital photographs were taken using a ZEISS AxioCam HR
attached to a ZEISS Axioskop 2 and occasionally optimised with
the software ZEISS AxioVision (Schubert 2005b, Heuchert et al.
2005).
Microscopic observations of isolates were made from colonies
cultivated for 7 d under continuous near-ultraviolet light at 25 °C
on SNA. Preparations were mounted in Shear’s solution (Crous et
al. 2009d). To study conidial development and branching patterns
of conidial chains, squares of transparent adhesive tape (Titan
the genuS Cladosporium
Ultra Clear Tape, Conglom Inc., Toronto, Canada) were placed on
conidiophores growing in the zone between the colony margin and
2 cm inwards, and mounted between two drops of Shear’s solution
under a glass cover slip. Conidial terminology follows Schubert
et al. (2007b). Wherever possible, 50 measurements (× 1 000
magniication, differential interference contrast microscopy, Zeiss
Axioscope 2 PLUS) were made of conidia with outliers given in
parentheses. For culture characteristics colonies were cultivated
on PDA, SNA, OA and MEA for 14 d at 25 °C in the dark, after
which the surface and reverse colours were rated using the charts
of Rayner (1970).
Low-temperature scanning electron microscopy (SEM)
SEM examinations of herbarium specimens, conducted at the
Institute of Zoology of the Martin-Luther-University in Halle, were
carried out to determine details of scar morphology and conidial
surface ornamentation. Specimens were excised from the host,
attached to aluminium pin stubs and then coated with a thin layer of
gold using a sputter coater SCD 004 (200 s in an argon atmosphere
of 20 mA, 30 mm distant from the electrode). Observations and
www.studiesinmycology.org
micrographs were made with a HITACHI S-2400 scanning electron
microscope with integrated camera (ilm: ILFORD PLUS 125).
Isolates of Cladosporium spp. were grown on SNA with 30 g
agar/L for 3–4 d at room temperature under black light. Relevant
parts of the small colonies with conidiophores and conidia were
selected under a binocular (× 10–50 magniication, Nicon SMZ
1500), excised with a surgical blade as small agar blocks (3 × 3 mm),
and transferred to a copper cup for snap-freezing in nitrogen slush.
Agar blocks were glued to the copper surface with frozen tissue
medium (KP-Cryoblock, Klinipath, Duiven, Netherlands) mixed with
1 part colloidal graphite (Agar Scientiic, Stansted, UK). Samples
were examined in a JEOL 5600LV scanning electron microscope
(JEOL, Tokyo, Japan) equipped with an Oxford CT1500 Cryostation
for cryo-electron microscopy (cryoSEM). Electron micrographs
were acquired from uncoated frozen samples, or after sputtercoating by means of a gold/palladium target for 3 times during 30
s. Micrographs of uncoated samples were taken at an acceleration
voltage of 3 kV, and consisted of 30 averaged fast scans (SCAN
2 mode), at 5 kV in case of the coated samples (PHOTO mode).
21
BenSch et al.
KEYS
Key to Cladosporium and morphologically similar genera ............................................................................................................................. 22
Keys to saprobic Cladosporium species ........................................................................................................................................................ 25
Key to saprobic Cladosporium species in vivo and in vitro ............................................................................................................................ 25
Key to species in the C. cladosporioides complex (in vitro) .......................................................................................................................... 27
Key to biotrophic and common saprobic foliicolous Cladosporium species in vivo based on morphology and ecology ............................... 30
Tabular key to biotrophic Cladosporium species based on host families and genera ................................................................................... 36
Key to fungicolous and lichenicolous Cladosporium species in vivo based on morphology and ecology ..................................................... 42
Tabular key to fungicolous and lichenicolous Cladosporium species based on hosts ................................................................................... 43
Cladosporium on aphids ................................................................................................................................................................................ 44
Key to Cladosporium and morphologically similar genera
[Genera bearing simple or branched acropetal chains of amero- to phragmosporous blastoconidia; based on Crous et al. (2007c), Braun &
Crous in Seifert et al. (2011)]
Notes: Genera that have to be considered segregations of Cladosporium s. lat. as previously comprehended and genera often confused
with the latter genus, i.e. to which species belong that have originally been assigned to Cladosporium s. lat., are marked by an asterisk (*).
1
1*
2
2*
3
Conidiophores and conidia hyaline ....................................................................................................................................................... 2
At least conidiophores pigmented ......................................................................................................................................................... 4
Conidia in simple chains ....................................................................................................................................................... Hormiactis
Conidia in branched chains ................................................................................................................................................................... 3
Conidiogenous cells mostly sympodial, with distinct conidiogenous loci (scars), thickened and darkened; conidia amero- to
phragmosporous; plant pathogenic, leaf-spotting fungi (Mycosphaerella anamorphs; Mycosphaerellaceae, Capnodiales)
...............................................................................................................................................................................................Ramularia*
[forming synnematous conidiomata; saprobic, see Sphaeridium]
3*
Terminal conidiogenous cells with denticle-like loci, giving rise to ramoconidia which form simple or branched conidial chains; lignicolous
....................................................................................................................................................................................... Hyalodendron
[Conidiophores dimorphic; mycelium, conidiophores and conidia at irst hyaline, later turning pale brown; conidia in short chains, see
Hyalodendriella]
4(1) Conidia distoseptate, in simple chains ........................................................................................................................................... Lylea
4*
Conidia aseptate or euseptate .............................................................................................................................................................. 5
5
Conidiophores little differentiated, micronematous to semi- macronematous; conidiogenous loci undifferentiated, truncate, neither
distinctly thickened nor darkened or only very slightly so ...................................................................................................................... 6
5*
Conidiophores well-differentiated, semimacronematous (but conidiogenous cells multilocal and/or conidiogenous loci well-differentiated)
to macronematous ............................................................................................................................................................................... 12
6
Conidiophores and conidia delicate, thin-walled, in long, easily disarticulating chains ......................................................................... 7
6*
Conidiophores and conidia robust, wall thickened, dark, conidial chains often seceding with dificulty ................................................ 9
7
Conidiophores semimacronematous, simple to often irregularly branched; conidia delicate, narrow, 1–3 μm wide, hyaline to pale
olivaceous ...................................................................................................................................................................... Polyscytalum
7*
Conidiophores unbranched, micronematous, integrated in ordinary hyphae, forming minute, lateral, monoblastic, determinate, peg-like
protuberances to semimacronematous, forming short lateral branches (conidiophores) with several inconspicuous to denticle-like
loci ......................................................................................................................................................................................................... 8
8
Phialidic synanamorphs often present, but sometimes also lacking; saprobic, rarely plant pathogenic, often human pathogenic
(Herpotrichiellaceae, Chaetothyriales) .................................................................................................................... Cladophialophora*
8*
Without phialidic synanamorphs; saprobic or plant pathogenic (Venturia, Venturiaceae)
.................................................................................................................................... Fusicladium s. lat. (incl. Pseudocladosporium)*
[similar, barely distinguishable taxa, also clustering in the Venturiaceae, but apart from the Venturia clade are tentatively referred to
as Anungitea until this genus will be resolved by sequencing of its type species]
9(6) Conidia aseptate, rarely 1-septate; lignicolous, on dead wood ............................................................................................ Xylohypha
9*
Conidia septate ................................................................................................................................................................................... 10
10 Conidia 1-septate, with a dark brown to blackish band at the septum; on dead wood ............................................................. Bispora
10* Conidia at least partly 2- to pluriseptate and/or without a dark brown to blackish band at the septum ............................................... 11
11 Conidia branched .................................................................................................................................................................. Taeniolina
11* Conidia unbranched ............................................................................................................................................................. Taeniolella
12(5) Conidiogenous loci and conidial hila distinctly coronate, i.e.composed of a central convex dome surrounded by a periclinal raised
rim, mostly at least somewhat protuberant (Davidiella anamorphs, Davidiellaceae, Capnodiales) ..................... Cladosporium s. str.
12* Conidiogenous loci non-coronate (either inconspicuous, thickened and darkened or denticle-like) ................................................... 13
22
the genuS Cladosporium
13
Mycelium, conidiophores and conidia at irst hyaline or sub-hyaline, later turning pale brown; conidiophores dimorphic, either
conidiogenous cells with a single conidiogenous locus (A-anamorph), giving rise to an ellipsoid cell (conidium?) which mostly remains
attached, base truncate, apex subacutely rounded, at times forming chains of such cells; or conidiophores with numerous aggregated
loci, inconspicuous to subdenticulate (B-anamorph); conidia in short chains, of mostly 2–3; isolated from Alnus in Europe
.................................................................................................................................................................................... Hyalodendriella*
13* Fruiting different; at least conidiophores consistently pigmented or conidiophores uniform or loci distinct; conidia mostly in long, often
branched chains .................................................................................................................................................................................. 14
14 Conidiophores with verruculose conidiogenous apices, otherwise smooth; conidia distinctly verruculose-verrucose; conidiogenous loci
and conidial hila inconspicuous ........................................................................................................................................................... 15
14* Conidiophores either smooth throughout or verruculose below and smooth above or verruculose throughout; and/or conidiogenous loci
conspicuous, i.e., thickened and darkened or denticle-like ................................................................................................................. 16
15 Conidiophores macronematous, unbranched, base swollen, with percurrent regenerative proliferations unrelated to conidiation;
conidiogenous cells terminal, occasionally also subterminal; conidia terminally and laterally formed, aseptate; saprobic on leaves
......................................................................................................................................................................................... Castanedaea*
15* Conidiophores little differentiated, semimacronematous, unbranched or only with short lateral branchlets, base undifferentiated, without
percurrent proliferations; conidiogenous cells terminal and occasionally intercalary-pleurogenous; conidia terminally and subterminally
formed, 0–2-septate; lignicolous, on decorticated wood ............................................................................................. Websteromyces
16(14)Conidiophores unbranched, with a simple terminal conidio-genous cell, non-geniculate-sinuous, subcylindrical to somewhat inlated at
the tip; conidiogenous loci terminal and lateral, inconspicuous or subconspicuous, neither thickened nor darkened, non-protuberant;
conidia attached with a very narrow, pointed hilum ............................................................................................................................. 17
16* Conidiophores with a branched terminal conidiogenous apparatus, composed of conidiogenous cells and/or ramoconidia or
conidiophores unbranched, with a single terminal conidiogenous cell or additional intercalary ones, but conidiogenous loci different,
conspicuous, thickened and darkened or denticle-like ........................................................................................................................ 18
17 Conidiophores with a distinct rhizoid-digitate base; tips of the conidiogenous cells somewhat swollen, usually unilaterally swollen or
somewhat curved; conidia solitary or only in very short unbranched chains; hyperparasitic on rusts ............................ Digitopodium*
17* Conidiophores without rhizoid-digitate base; tips of the conidiogenous cells subcylindrical to somewhat swollen, but swellings not
unilateral and not curved; conidia solitary and in simple or branched chains; associated with leaf spots ............ Rachicladosporium*
18(16)Conidiophores in synnematous conidiomata ...................................................................................................................................... 19
18* Synnemata lacking .............................................................................................................................................................................. 20
19 Conidiogenous cells with a single or several truncate to sub-denticulate, relatively broad conidiogenous loci; conidia with truncate, lat
hila; on wood, resin ............................................................................................................................................................... Sorocybe*
19* Conidiogenous loci with few, mostly 1–2 conidiogenous loci formed as minute spicules; conidia with narrow hila (shallowly apiculate);
plant pathogenic, causing bud blast and twig blight ................................................................................................................. Seifertia
20(18)Conidiophores unbranched or occasionally branched; conidio-genous cells distinctly inlated, ampulliform, doliiform or clavate, nondenticulate; conidia at least partly globose, dark brown when mature; colonies effuse, dark; wood-inhabiting ...... Phaeoblastophora
20* Conidiogenous cells not inlated, if somewhat inlated, loci denticle-like or conidia non-globose ....................................................... 21
21 Conidiophores penicillate, i.e., with an unbranched stipe and distinct terminal branched ‘head’ composed of branchlets, conidiogenous
cells and/or ramoconidia ..................................................................................................................................................................... 22
21* Conidiophores non-penicillate, i.e., irregularly and loosely branched, branchings not conined to the apical portion, sometimes only with
short lateral branchlets, or unbranched ............................................................................................................................................... 27
22 Penicillate apex simple, only composed of a single terminal conidiogenous cell giving rise to several ramoconidia which form secondary
ramoconidia and conidia ................................................................................................................... Penidiella p.p. [P. strumelloidea]*
22* Penicillate apex more complex, composed of true branchlets and/or conidiogenous cells and ramoconidia ..................................... 23
23 Conidiophores with a compact, dense, subglobose to broadly ovoid head; conidiogenous loci and conidial hila unthickened or almost
so, but distinct by being darkened-refractive [fruiting dimorphic, periconioid branched conidiophores formed on overwintered stems of
Paeonia spp., unbranched cladosporioid conidiophores on leaf spots, biotrophic] (belonging to the Capnodiales) ........ Graphiopsis*
23* Penicillate apex looser, neither compact nor subglobose ................................................................................................................... 24
24 Branched head composed of short branchlets and conidiogenous cells; ramoconidia lacking; conidiogenous cells subcylindrical to
subclavate, non- or only slightly geniculate; conidiogenous loci usually numerous and aggregated, terminal and lateral, non-protuberant,
lat, conspicuous, thickened and darkened, at least around the rim; conidia solitary or in short chains ............................ Periconiella
24* Ramoconidia present; conidiogenous cells distinct, sympodial, somewhat geniculate or subdenticulate; conidiogenous loci
inconspicuous or somewhat protruding, denticle-like, unthickened or almost so, not or somewhat darkened-refractive; conidia in long,
often branched chains ......................................................................................................................................................................... 25
25 Branched apex composed of short branchlets consisting of conidiogenous cells or ramoconidia, in pairs or whorls of 3–4, mostly
distinctly constricted at the base; hyperparasitic on Asterina spp. ............................................................................ Parapericoniella*
25* Branched apex distinct, composed of branchlets, conidio-genous cells and/or ramoconidia; if true branchlets lacking, conidiogenous
cells and ramoconidia not in whorls and not distinctly constricted at the base; saprobic or biotrophic ............................................... 26
26 Penicillate apex of the conidiophores loosely to densely ranched, occasionally metula-like, base of the conidiophores simple,
undifferentiated; saprobic or biotrophic (Teratosphaeriaceae, Capnodiales) ....................................................................... Penidiella*
26* Penicillate apex always dense, metula-like, base of the conidio-phores swollen or lobed, often with rhizoid hyphae; plant pathogenic
[on banana] (Chaetothyriales) ........................................................................................................................... Metulocladosporiella*
27(21)Conidiophores simple or branched; septa of the conidiophores and conidia becoming thick-walled and dark; conidiogenous loci
www.studiesinmycology.org
23
BenSch et al.
subdenticulate, somewhat thickened and conspicuously darkened-refractive; cultures producing ample amounts of volatile metabolites
causing skin irritation after exposure to the fungus; saprobic (isolated from mouldy paint) ............................... Toxicocladosporium*
27* Without conspicuously thickened-darkened septa; cultures without toxic, volatile metabolites .......................................................... 28
28 Conidiogenous loci conspicuous, distinctly thickened and darkened (visible as small dark circles when viewed upon the scar),
sometimes on small shoulders formed by sympodial proliferation, but not distinctly denticulate (Capnodiales) ................................. 29
28* Conidiogenous loci inconspicuous or conspicuous by being denticle-like, not or barely thickened, not darkened or at most upper
truncate end very slightly thickened and somewhat darkened-refractive ............................................................................................ 32
29 Mycelium smooth; conidiophores and conidia smooth or almost so, at most faintly rough-walled; conidiophores solitary, fasciculate,
sporodochial to synnematous; biotrophic, usually leaf-spotting (Mycosphaerella anamorphs, Mycosphaerellaceae, Capnodiales)
............................................................................................................ Passalora emend. (incl. Mycovelosiella, Phaeoramularia, etc.)*
29* At least mycelium distinctly verruculose .............................................................................................................................................. 30
30 Mycelium, conidiophores and conidia coarsely verruculose-verrucose; conidial shape variable, often irregular; isolated from a lichen
[Dirina] .............................................................................................................................................................. Verrucocladosporium*
30* Mycelium verruculose; conidiophores often smooth, sometimes somewhat rough-walled, but not coarsely verruculose-verrucose;
conidia smooth to distinctly verruculose; biotrophic, often leaf-spotting .............................................................................................. 31
31 Conidiogenous loci and conidia hila truncate, planate ....................................................................................................... Zasmidium*
31* Conidiogenous loci and conidia hila pileate .................................................................................................................. Stenella s. str.*
32(28)Conidiophores with swollen, often lobed base .................................................................................................................................... 33
32* Base of the conidiophores not swollen, at most slightly swollen, but not lobed .................................................................................. 36
33 Conidia septate ................................................................................................................................................................................... 34
33* Conidia aseptate ................................................................................................................................................................................. 35
34 Conidiophores with a single, terminal, monoblastic, determinate conidiogenous cell giving rise to a single ramoconidium that forms
simple or branched chains of conidia ................................................................................................................ Parapleurotheciopsis*
34* Terminal conidiogenous cells polyblastic, with several denticle-like conidiogenous loci ........... Anungitea p.p. (e.g., A. longicatenata)
35(33)Conidiogenous cells terminal, monoblastic, with a single ramoconidium giving rise to conidial chains or occasionally with 2(–3) denticlelike loci; base of the conidiophores often with rhizoid hyphae .............................................................................. Rhizocladosporium*
35* Conidiogenous cells polyblastic, with two or several denticle-like loci; base of the conidiophores without rhizoid hyphae
................................................................................................................................................................................. Subramaniomyces
36(32)Conidiophores unbranched, with a terminal monoblastic conidiogenous cell, determinate or percurrent .......................................... 37
36* Conidiophores branched or unbranched, but conidiogenous cells at least partly polyblastic .............................................................. 39
37 Conidiogenous cell giving rise to a single ramoconidium which forms simple or branched chains of 0(–1)-septate secondary conidia
........................................................................................................................................... Parapleurotheciopsis p.p. (P. coccolobae)
37* Conidiogenous cells giving rise to simple conidial chains without ramoconidia; conidia septate ........................................................ 38
38 Conidiophores sometimes with percurrent proliferations; conidiophores and conidia with somewhat thickened, dark walls; conidia
1–10-septate, width usually exceeding 4 μm ................................................................................................................. Heteroconium
38* Percurrent proliferations lacking; conidiophores and conidia delicate, thin-walled and paler; conidia usually 0–1(–3)-septate and
narrow, usually below 4 μm wide (Chaetothyriales) ...................................................... Cladophialophora p.p. (e.g., C. chaetospira)*
[similar anamorphs of the Venturiaceae, see Fusicladium (incl. Pseudocladosporium)]
39(36)Conidiophores often branched; conidiogenous loci distinctly denticle-like or subdenticulate; conidia aseptate; lignicolous, on dead
wood, resin or isolated from hydrocarbone-rich substrates (jet-fuel, cosmetics, etc.) ......................................................................... 40
39* Either with unbranched conidiophores or conidiogenous loci not distinctly denticle-like, or conidia septate, or on other substrates
............................................................................................................................................................................................................. 43
40 Conidiogenous cells distinctly denticulate; conidia rather broad, about 7–13 μm ........................................................... Haplotrichum
40* Conidiogenous cells non-denticulate or at most subdenticulate; conidia narrower, about 3–6 μm ..................................................... 41
41 Colonies effuse, dense, but felted, black, brittle and appearing carbonaceous when dry; conidiophores solitary, brown; conidiogenous
cells terminal and pleurogenous; conidia pale to dark brown, lateral walls conspicuously thicker than the hila; on conifer resin
................................................................................................................... Sorocybe (mononematous form, Hormodendrum resinae)*
41* Colonies effuse, dense, resupinate, hypochnoid, powdery, chocolate-brown and/or conidiophores lightly pigmented; conidia subhyaline
to lightly pigmented and/or lateral walls not thickener than poles; on dead wood or isolated from hydrocarbone-rich substrates (jet-fuel,
cosmetics, etc.) ................................................................................................................................................................................... 42
42 Colonies effuse, dense, resupinate, hypochnoid, powdery, chocolate-brown; conidiophores smooth; conidia subhyaline to very pale
yellowish, hila very thin; on dead wood .................................................................................................................... Parahaplotrichum
42* Colonies neither resupinate nor hypochnoid; conidiophores warty; lateral walls of the conidia not thicker than the hila; isolated from
hydrocarbone-rich substrates (jet-fuel, cosmetics, etc.), creosote fungus
................................................................................................. Amorphotheca anamorph (Cladosporium avellaneum, ‘Hormoconis’)*
43(39)Conidiophores simple or branched; conidiogenous cells monoblastic or occasionally polyblastic; conidiogenous loci subdenticulate,
neither thickened nor darkened, forming simple or branched chains of regular conidia, uniform in shape, size and septation
............................................................................................................................................................................................ Septonema*
43* Conidia not uniform in shape, size and septation; conidiogenous loci lat-tipped, subdenticulate, unthickened or slightly so, not to
somewhat darkened-refractive ............................................................................................................................................................ 44
44 Conidiophores simple or branched; in culture forming abundant chlamydospores; mostly soil-borne and heat-resistant
(Teratosphaeriaceae, Capnodiales) ....................................................................................................................................... Devriesia*
24
the genuS Cladosporium
44*
45
45*
46
46*
47
47*
48
48*
Without chlamydospores in culture; phylogenetically distinct .............................................................................................................. 45
Conidiophores dimorphic; conidia mostly aseptate, hila inconspicuous, neither thickened nor darkened (Pleosporales)
............................................................................................................................................................................. Ochrocladosporium*
Conidiophores either uniform or conidia at least partly septate or hila more conspicuous by being slightly thickened or at least somewhat
darkened-refractive; phylogenetically distinct ...................................................................................................................................... 46
Phialidic synanamorphs often present, but sometimes also lacking; saprobic, rarely plant pathogenic, often human pathogenic
(Herpotrichiellaceae, Chaetothyriales) .................................................................................................................... Cladophialophora*
Without phialidic synanamorphs; saprobic or plant pathogenic; phylogenetically different ................................................................. 47
Conidiophores usually unbranched (Venturia, Venturiaceae) .............................................................................................................. 48
Conidiophores simple to often irregularly branched; conidia delicate, narrow, 1–3 μm wide, hyaline to pale olivaceous (not belonging
to the Venturiaceae) ...................................................................................................................................................... Polyscytalum
Saprobic species forming long conidial chains ................................................................................................... Pseudocladosporium
Biotrophic usually leaf spotting species, conidia formed singly or in rather short chains ................................................. Fusicladium*
[similar, barely distinguishable taxa, also clustering in the Venturiaceae, but apart from the Venturia clade are tentatively referred to as
Anungitea until this genus will be resolved by sequences of its type species]
Keys to saprobic Cladosporium species
Key to saprobic Cladosporium species in vivo and in vitro
Forming large stromata in vivo, 20–100 μm diam, later expanding or conluent, up to 500 μm diam; conidiophores very numerous, in
large, dense fascicles, 100–250 × 3–7 μm, straight to geniculate-subnodulose; conidia solitary or in simple chains, 8–25 × 5–10 μm,
coarsely verrucose, verrucae distant, up to 2.5 μm, and up to 1 μm diam; on dead stems of Zinnia ................................ C. oblongum
1*
Stromata lacking or much smaller; conidiophores shorter or narrower, or conidia narrower, in branched chains, or smooth, or echinulate,
or densely verruculose to verrucose ..................................................................................................................................................... 2
2
Conidiophores distinctly torulose-nodulose with conidiogenous loci conined to swellings or conidiophores strongly geniculate-sinuous,
subnodulose due to unilateral swellings at lateral shoulders caused by sympodial proliferations (geniculations) ................................ 3
2*
Conidiophores non-nodulose, sometimes geniculate, but geniculations not so strong, without distinct unilateral swellings .............. 10
3
Conidia smooth or almost so (light microscopy); conidiophores up to about 400 μm long and 4–5 μm wide, swellings usually numerous,
regular and distant, ca. 6–8 μm diam; conidia up to 25 × 3–8 μm ................................................................................... C. oxysporum
3*
Conidia distinctly verruculose to verrucose or spinulose ....................................................................................................................... 4
4
Conidiophores strongly geniculate-sinuous, subnodulose due to unilateral swellings at lateral shoulders caused by sympodial
proliferations (geniculations); conidiophores in vitro uniformly macronematous; conidia solitary or only in short unbranched chains of
up to three conidia, 9–21 × (5–)6–8 μm ........................................................................................................................... C. sinuosum
4*
Conidiophores in vitro both macronematous and micronematous, macronematous conidiophores distinctly torulose-nodulose; conidia
in long, often branched chains, if solitary conidia wider, up to 12(–15) μm ........................................................................................... 5
5
Macronematous conidiophores in vivo up to 8 μm wide, nodulose swellings 5–12 μm diam; secondary ramoconidia in vitro (5–)6–9
(–10) μm wide, in vivo (6–)7–12(–15) μm, in vivo at least with some very broad conidia formed singly or only in very short, unbranched
chains, unchanged with age ....................................................................................................................................... C. macrocarpum
5*
Macronematous conidiophores somewhat narrower, in vitro (1.5–)2.5–5 μm, nodulose swellings also somewhat narrower, 3–8 μm
diam; conidia narrower, 2.5–8 μm wide, or conidia 3–6 μm wide, but with age becoming wider, (3.5–)5–9(–11) μm, darker and more
thick-walled ............................................................................................................................................................................................ 6
6
Swellings of the macronematous conidiophores 3–6.5 μm diam; conidia 4–17(–22) μm long, ornamentation variable, but usually
densely echinulate, with spines up to 0.8 μm long ......................................................................................................... C. subinlatum
6*
Swellings of the macronematous conidiophores (4–)5–8(–9) μm diam; conidia longer, up to 25(–35) μm, ornamentation verruculose
to verrucose, but not echinulate ............................................................................................................................................................ 7
7
Conidia formed by macronematous conidiophores 3–33 × (2–)3–6(–7) μm, with age becoming wider, (3.5–)5–9(–11) μm, darker and
more thick-walled .......................................................................................................................................................... C. herbaroides
7*
Conidia formed by macronematous conidiophores uniform, with age not becoming wider, darker and more thick-walled ................... 8
8
Conidiophores with a single or usually numerous nodulose swellings in short succession, giving the stalk a knotty/gnarled appearance;
small terminal conidia 4–10 × 3–5(–6) μm; intercalary conidia 6–16 × 4–6 μm; secondary ramoconidia 12–25(–35) × (3–)5–7(–9) μm,
0–1(–3)-septa .................................................................................................................................................................... C. herbarum
8*
Conidia narrower, secondary ramoconidia 10–25(–30) × 3–5(–7) or 4–27 × 3–6.5 μm, but then up to 6-septate ................................ 9
9
Conidiophores in vivo uniform, not dimorphic, unbranched, usually with small, terminal, head-like swellings, sometimes also with
additional intercalary swellings; small terminal conidia 4–9 × 2.5–3.5 μm; secondary ramoconidia and occasionally formed ramoconidia
10–25(–30) × 3–5(–7) μm .......................................................................................................................... C. allicinum (= C. bruhnei)
9*
Conidiophores in vivo dimorphic, with very short as well as long conidiophores, unbranched or branched, with several swellings;
ramoconidia absent; secondary ramoconidia 4–27 × 3–6.5 μm, 0–6-septate ........................................................... C. dieffenbachiae
10(2) Small terminal conidia globose to subglobose-ovoid, almost smooth to verruculose; conidiophores simple or frequently branched, or
conidia echinulate-digitate, spines up to 1.3 μm long, formed by unbranched conidiophores (mainly C. sphaerospermum complex)
............................................................................................................................................................................................................ 11
1
www.studiesinmycology.org
25
BenSch et al.
10*
Small terminal conidia non-globose, rather ellipsoid-ovoid, obovoid, fusiform, limoniform or short subcylindrical (if with some intermixed
subglobose conidia, conidiophores unbranched or even micronematous) .......................................................................................... 17
11 Conidia echinulate-digitate, spines up to 1.3 μm long, formed by unbranched conidiophores ....................................... C. spinulosum
11* Conidia smooth or almost so to verruculose or verrucose .................................................................................................................. 12
12 Secondary ramoconidia 0–3(–4)-septate, septa thickened and darkened .......................................................................................... 13
12* Secondary ramoconidia 0–1(–2)-septate, septa neither thickened nor darkened ............................................................................... 14
13 Conidiophores in vitro 2–3.5(–5.5) μm wide, usually unbranched; small terminal conidia 2–5(–6) × 2–3(–5) μm; secondary ramoconidia
7–25(–31) × 2–3.5(–6.5) μm; ramoconidia sporadically formed .................................................................................... C. halotolerans
13* Conidiophores mostly somewhat wider, 2.5–4.5(–6) μm, in vitro and in vivo often branched; small terminal conidia mostly wider,
(2–)3–5(–7) × (2–)3–3.5(–4.5) μm; secondary ramoconidia (4–)8–22(–37.5) × (2–)3–4(–5) μm; ramoconidia often formed, up to 40 μm
long, with up to ive septa, commonly beaked (alternarioid) on MEA and PDA ................................................... C. sphaerospermum
14(12)Conidia and secondary ramoconidia irregularly verruculose to sometimes loosely verrucose; radial growth on PDA at 25 °C after
14 d typically less than 5 mm ............................................................................................................................................ C. langeronii
14* Conidia and secondary ramoconidia smooth to minutely verruculose; radial growth on PDA at 25 °C after 14 d typically more than
10 mm ................................................................................................................................................................................................. 15
15 Conidiophores (3–)3.5–4(–7.5) μm wide, thick-walled; conidiogenous loci and conidial hila 0.5–2 μm diam; ramoconidia sometimes
formed (with truncate base, up to 2 μm wide, i.e., base without coronate hilum); no growth observed after 14 d at 30 °C on MEA
................................................................................................................................................................................ C. psychrotolerans
15* Conidiophores usually narrower, 2–4 μm wide, wall less thickened; conidiogenous loci and conidial hila 0.5–1.5 μm diam; ramoconidia
rarely formed; colonies showing at least weak growth after 14 d at 30 °C on MEA ............................................................................ 16
16 Secondary ramoconidia (4–)6.5–13(–24.5) μm long; no visible colony growth after 14 d at 10 °C on MEA ............... C. dominicanum
16* Secondary ramoconidia (3.5–)5.5–19(–42) μm long; radial growth of colonies after 14 d at 10 °C on MEA more than 5 mm
................................................................................................................................................................................................... C. velox
17(10)Conidiophores in vitro micronematous, little differentiated, short, (5–)10–50(–60) μm long .................................................. C. salinae
17* Conidiophores in vitro micronematous as well as macronematous or only macronematous, in vivo usually only macronematous ... 18
18 Conidiophores very long, up to about 500 μm, unbranched, iliform-setiform, pluriseptate, wall somewhat thickened, not pale, at least
medium brown; conidiogenous cells terminal, mostly somewhat swollen, often unilaterally swollen; conidia smooth to minutely
verruculose ................................................................................................................................................................... C. tenuissimum
18* Conidiophores much shorter, not iliform-setiform, or conspicuously geniculate, or with terminal as well as intercalary conidiogenous
cells and loci ........................................................................................................................................................................................ 19
19 Conidia smooth, rarely some conidia minutely rough-walled .............................................................................................................. 20
19* Conidia verruculose to verrucose or echinulate .................................................................................................................................. 27
20 Conidiophores 15–500 × 2.5–9(–11) μm, arising from supericial hyphae, pluriseptate, frequently dichotomously or subdichotomously
branched; conidiogenous cells usually terminal and somewhat swollen; conidia 2–32 × 2–7 μm, 0–3(–4)-septate ............. C. varians
20* Conidiophores shorter, not dichotomously branched or conidia smaller or with fewer septa .............................................................. 21
21 Conidiophores usually cylindrical-iliform, non-geniculate-sinuous, in vivo usually arising from immersed hyphae or swollen hyphal
cells, usually only with a single terminal conidiogenous cell, not or barely swollen, but occasionally subdenticulate, only with a single
or few conidiogenous loci; conidia usually 2–5 μm wide and 0–1-septate ........................... C. cladosporioides complex (see below)
21* Conidiophores geniculate-sinuous or with intercalary conidiogenous cells and conidiogenous loci, or terminal conidiogenous cells
distinctly swollen, or conidia different, wider or with more septa ......................................................................................................... 22
22 Conidiophores fasciculate, usually strongly geniculate-sinuous, 50–300 × 3–8 μm; ramoconidia present, 8–18 × 3–7 μm, with truncate
base, 2–4 μm wide, 0–3-septate; conidia 3–15 × 2.5–5.5 μm, 0–1-septate; only known from dead leaves of Magnolia ... C. delectum
22* Conidiophores straight, subcylindrical to slightly geniculateinuous; ramoconidia lacking ................................................................... 23
23 Conidiophores 7–126 × 3–5(–6) μm; conidia 2–6 μm wide, 0–1-septate, thin-walled, smooth; cells without distinct lumens
.......................................................................................................................................................................................... C. pannosum
23* Conidiophores up to 250(–300) × 3–9(–10) μm; conidia (2–)3–10 μm wide, 0–5-septate, wall thickened; cells of the hyphae,
conidiophores and conidia often with distinct, subglobose to irregular lumens ................................................................................... 24
24 In vivo mycelium internal and external, supericial; conidiophores arising from immersed hyphae or swollen hyphal cells or solitary,
arising from supericial hyphae, 10–120 × 3–8 μm; conidia 8–40 × 5–10 μm ..................................... C. astroideum var. astroideum
24* In vivo mycelium mostly internal; conidiophores only arising from immersed hyphae, swollen hyphal cells or stromatic hyphal
aggregations, 20–250(–300) μm long; conidia narrower, (2–)3–7(–9) μm .......................................................................................... 25
25 Conidiophores often branched, especially in the upper portion frequently branched .................................................. C. sarmentorum
25* Conidiophores usually unbranched ..................................................................................................................................................... 26
26 Conidia (2–)3–6(–7) μm; conidiogenous loci and hila 0.5–1.5(–2) μm diam .................................................................. C. delicatulum
26* Conidia 3–8(–10) μm wide; conidiogenous loci and hila 1–2(–2.5) μm diam ..................................................................... C. nigrellum
27(19)Conidia at irst smooth, but later mixed with verruculose conidia; cells of the hyphae, conidiophores and conidia often with distinct,
subglobose to irregularly shaped lumens ............................................................................................................................................ 28
27* All conidia verruculose to verucose or echinulate ............................................................................................................................... 29
28 In vivo mycelium internal and external, supericial; conidiophores arising from immersed hyphae or swollen hyphal cells or solitary,
arising from supericial hyphae, 10–120 × 3–8 μm; conidia 8–40 × 5–10 μm ..................................... C. astroideum var. astroideum
28* In vivo mycelium internal; conidiophores only arising from immersed hyphae, swollen hyphal cells or stromatic hyphal aggregations,
20–250(–300) μm long; conidia narrower, (2–)3–7(–8) μm ............................................................................................ C. delicatulum
26
the genuS Cladosporium
29(27)Conidia with distinctly echinulate, spiny ornamentation, spines 0.5–1.3 μm long; hila usually situated on small peg-like prolongations
or denticles ..................................................................................................................................................................... C. spinulosum
29* Conidia verruculose to verrucose ........................................................................................................................................................ 30
30 Conidiophores in vivo fasciculate, 10–70 × 3–7 μm, arising from stromata, 10–60 μm diam, later enlarged or conluent, up to 150 μm
diam; conidia solitary or in unbranched chains, 5–26 × 3–5.5 μm, 0–3-septate; on dead legumes of Phaseolus ....... C. leguminicola
30* Stromata lacking or much smaller; conidia in branched chains ........................................................................................................... 31
31 Conidiophores cladosporioides-like, 100–400 × 3–8 μm, usually iliform, non-geniculate-sinuous, only with a single terminal unswollen
conidiogenous cell, with a single or few conidiogenous loci, occasionally subdenticulate; conidia (5–)8–15 × 3–6 μm; secondary
ramoconidia up to 25 × 8.5 μm ........................................................................................................................................... C. auriculae
31* Conidiophores much shorter, geniculate-sinuous, with terminal as well as intercalary or pleurogenous conidiogenous cells ............ 32
32 Conidiophores 10–120 × 3–6 μm, frequently verruculose, at least towards the base; conidia (5–)8–35(–40) × 3–9 μm, 1–4(–6)-septate,
thick-walled, often with constrictions at the septa, occasionally with oblique to longitudinal septa ..... C. astroideum var. catalinense
32* Conidia 2–6(–7) μm wide, 0–3(–4)-septate, thin-walled, not or barely constricted at the septa, all septa vertical, not longitudinal .... 33
33 Mycelium in vitro dimorphic, narrow hyphae 1–3 μm wide, hyaline or subhyaline, thin-walled, hyphae of the second type wider, 3.5–8
(–9) μm wide, pale to dark greyish olivaceous to olivaceous-brown, thick-walled, wall sometimes even two-layered, 1(–1.5) μm
wide, hyphae appearing consistently enveloped in polysaccharide-like material or covered by a slime coat; conidiophores usually
several times slightly to usually distinctly geniculate towards the apex, with up to 14 conidiogenous loci; secondary ramoconidia 13–20
× 4–5 μm, 0–3-septate; isolated from a lichen, probably lichenicolous .......................................................................... C. antarcticum
33* Mycelium in vitro not dimorphic; neither enveloped in polysaccharide-like material nor covered by a slime coat [if hyphae with extracellar
polysaccharide-like material, secondary ramoconidia much smaller, (5–)6–11(–22) × 2.5–3 μm, 0(–1) μm]; or only known in vivo (on
dead leaves of Typha), secondary ramoconidia 15–30 × 4–6(–7) μm, (0–)1–3(–4)-septate, conidiophores non or only slightly geniculate,
with few conidiogenous loci; saprobic species .................................................................................................................................... 34
34 Small terminal conidia ellipsoid-subcylindrical, narrowly obovoid, limoniform to fusiform, but not globose ........................................ 35
34* Small terminal conidia globose to subglobose .................................................................................................................................... 37
35 Terminal conidia ellipsoid-subcylindrical, septate conidia and secondary ramoconidia 15–30 × 4–6(–7) μm, (0–)1–3(–4)-septate;
saprobic on dead leaves of Typha ................................................................................................................................. C. heleophilum
35* Terminal conidia narrowly obovoid, limoniform to fusiform, secondary ramoconidia narrower, 2.5–5 μm, 0–1(–2)-septate ............... 36
36 Small terminal conidia variable, narrowly obovoid, limoniform to fusiform; secondary ramoconidia (13–)17–32(–37) × 3–5(–6) μm,
0–1(–2)-septate; in vitro mycelium without extracellar polysaccharide-like material .................................................. C. subtilissimum
36* Small terminal conidia fusiform; secondary ramoconidia smaller, (5–)6–11(–22) × 2.5–3 μm, 0(–1)-septate; in vitro mycelium with
extracellar polysaccharide-like material ............................................................................................................................. C. fusiforme
37(34)Conidiophores usually with numerous conidiogenous loci forming sympodial clusters of pronounced scars at the apex, sometimes
up to 10 or even more denticle-like loci; conidia 3–20(–28) × 2.5–5(–6) μm, 0–1(–2)-septate, often with several apically crowded hila,
up to 7(–9) ........................................................................................................................................................................... C. tenellum
37* Conidiophores usually only with few conidiogenous loci, mostly 1–3; conidia longer and narrower, 2.5–32 × 2–4(–5) μm, 0–3-septate,
usually only with 1–3 distal hila .................................................................................................................................. C. ramotenellum
Key to species in the C. cladosporioides complex (in vitro)
Morphological features used in the key to distinguish the species treated in this study were determined by light microscopy after 7 to 9 d
growth at 25 ºC on SNA, and culture characteristics after 14 d incubation on PDA. Cladosporium uredinicola and C. vignae are not included
in the key because of problems with type material, identity of putatively representative specimens, and since isolates of these species did
not sporulate during the course of the examinations (Bensch et al. 2010), and measurements given in literature were made only on PDA and
are therefore only partly comparable. Terminology used for conidial types, scars and surface ornamentation follow Schubert et al. (2007b). To
relect the morphological variability of some of the species, especially with regard to surface ornamentation of conidia, these species are listed
twice or up to three times in the key, e.g. C. exile. The C. cladosporioides s. lat. complex (see couplet 35 in the key), includes species that are
morphologically close to C. cladosporioides but still distinguishable due to a combination of subtle features as well as C. cladosporioides s.
str. and morphologically indistinguishable but phylogenetically distinct lineages of the latter species. Lineages of C. cladosporioides s. lat. are
phylogenetically closer to some of the species covered below than they are to C. cladosporioides s. str., even though they are morphologically
not distinguished from C. cladosporioides s. str. (Bensch et al. 2010, ig. 1).
1
1*
2
2*
3
3*
4
4*
Conidia inely verruculose to coarsely verrucose or irregularly rough-walled ........................................................................................ 2
Conidia smooth or almost smooth ....................................................................................................................................................... 15
Conidia distinctly ornamented, verruculose to coarsely verrucose or irregularly rough-walled ............................................................. 3
Conidia less ornamented, almost smooth to asperulate or minutely verruculose, sometimes irregularly rough-walled ........................ 7
Conidia frequently septate, with 0−3 septa; surface with coarse verrucae up to 1 μm high ...................... C. verrucocladosporioides
Conidia mainly 0−1-septate, occasionally with a second septum; verrucae lower, only up to 0.5 μm high ........................................... 4
Conidiophores up to 430 μm long, smooth; small terminal conidia globose, subglobose or obovoid, broad, 4.5−6 μm wide
........................................................................................................................................................................................... C. acalyphae
Conidiophores shorter, up to 200 μm long, usually shorter, almost smooth to minutely verruculose or irregularly rough-walled; small
www.studiesinmycology.org
27
BenSch et al.
terminal conidia obovoid, ellipsoid, ovoid, rarely subglobose, 2.5−4.5 μm wide ................................................................................... 5
Conidiophores macronematous, (2.5−)3.5−5.5 μm wide, walls thickened, 0.5−1(−1.5) μm wide, sometimes even appearing to be twolayered; small terminal conidia 5−6 μm long .......................................................................................................... C. pini-ponderosae
5*
Conidiophores macro-, semimacro- and micronematous, narrower, (1.5−)2.5−4 μm wide, walls unthickened or only slightly thickened,
about 0.5 μm wide; small terminal conidia longer, 4−9 μm long ............................................................................................................ 6
6
Ramoconidia 4−5 μm wide, aseptate; conidia inely verruculose to usually verruculose, occasionally distinctly verrucose; conidiogenous
loci and hila (0.5−)0.8−2(−2.2) μm diam ......................................................................................................................... C. chubutense
6*
Ramoconidia narrower, 2.8−4 μm, 0−2-septate; conidia mostly distinctly verruculose-rugose or irregularly rough-walled; conidiogenous
loci and hila narrower, 0.5−1.5 μm diam ....................................................................................................................... C. exasperatum
7(2) Terminal unbranched part of the branched conidial chains usually very long with up to 8(−10), sometimes up to 17 conidia .............. 8
7*
Conidia in densely branched chains, terminal unbranched part of the chains much shorter with 1−4 conidia ................................... 11
8
Conidia inversely coloured with small terminal and intercalary conidia being slightly darker than secondary ramoconidia, ramoconidia
and conidiophores; small terminal conidia (3−)5−8.5 μm long, intercalary conidia (5−)7−20 μm long; small terminal and intercalary
conidia in delicate, loose chains, minutely verruculose or irregularly rough-walled, rugose, secondary ramoconidia and ramoconidia
smooth ............................................................................................................................................................................ C. inversicolor
8*
Conidia not inversely coloured, small terminal and intercalary conidia paler or concolorous with secondary ramoconidia, ramoconidia
and conidiophores; small terminal and intercalary conidia shorter, 4−7(−8) μm and (5−)6.5−10(−12) μm, respectively; no differences in
ornamentation between smaller conidia and secondary ramoconidia ................................................................................................... 9
9
Conidiophores 45−210(−360) μm long, pluriseptate, with up to 12 septa; secondary ramoconidia (7.5−)9−26(−37) × (2.5−)3−5 μm,
0(−1)-septate ................................................................................................................................................................. C. asperulatum
9*
Conidiophores shorter, up to 100(−115) μm long, 0−4(−5)-septate; secondary ramoconidia somewhat shorter and narrower, 8−20(−23)
× (2.5−)3−4 μm, 0−1(−2)-septate ........................................................................................................................................................ 10
10 Conidiophores macronematous, (2.5−)3.5−4.5(−5) μm wide, thick-walled, walls up to 1 μm wide; conidiogenous cells geniculate,
subnodulose with unilateral swellings or occasionally nodulose, with up to six loci crowded at the apex; conidia smooth or almost so to
inely verruculose; on Myrtaceae ................................................................................................................................. C. myrtacearum
10* Conidiophores macro- and micronematous, slightly narrower, 2−4(−4.5) μm wide, walls slightly thickened, up to 0.5 μm; conidiogenous
cells non-nodulose, occasionally geniculate, usually with a single apical scar, sometimes with 2−3 conidiogenous loci at the apex;
conidia smooth to minutely verruculose or often irregularly rough-walled; on Cortaderia ................................................ C. colombiae
11(7) Macronematous conidiophores 2.5−5(−6) μm wide; secondary ramoconidia 5−17 (−24) × (2−)3−4.5 μm .............. C. phyllactiniicola
11* Macronematous conidiophores narrower, (1.5−)2−4(−5) μm; secondary ramoconidia longer and/or narrower, 10−30(−34) × 2−3.5(−4)
μm ....................................................................................................................................................................................................... 12
12 Conidiophores (1.5−)2−3.5(−4) μm wide, subhyaline, pale olivaceous to pale olivaceous-brown; conidiogenous loci and hila narrow,
(0.8−)1−1.5(−1.8) μm diam .................................................................................................................................................................. 13
12* Conidiophores somewhat wider, (2−)3−4(−5) μm, and darker, pale to medium olivaceous-brown; secondary ramoconidia somewhat
wider, 2.5−3.5(−4) μm; conidiogenous loci and hila, 0.5−2 μm diam .................................................................................................. 14
13 Ramoconidia 25−45 × 2.5−3(−4.5) μm; secondary ramoconidia 6−30(−34) × 2−3(−3.5) μm, 0−1(−3)-septate, 4−16(−19) × 2−3(−3.5)
μm ............................................................................................................................................................................... C. perangustum
13* Ramoconidia 17–20(–28) × (2–)3(–4) μm, secondary ramoconidia (5–)10–15(–20) × (3–)3.5(–4) μm, aseptate ...... C. phaenocomae
14 Ramoconidia 17−41 μm long with a broadly truncate base, 2.5−3 μm wide; small terminal conidia 3.5−5(−5.5) × 2−3 μm, intercalary
conidia (4−)5−8(−9) μm long; conidiogenous loci and hila 0.5−2 μm diam ................................................................................ C. exile
14* Ramoconidia up to 34 μm, base 2−2.5 μm wide; small terminal conidia 3.5−4.5(−5) × 2−2.2(−2.5) μm, intercalary conidia longer, 5−13
μm; conidiogenous loci and hila slightly narrower, 0.5−1.8 μm diam .............................................................................. C. scabrellum
15(1) Macronematous conidiophores nodulose or nodose with swellings usually being quite apart from each other; conidiogenous loci
usually restricted to swellings .............................................................................................................................................................. 16
15* Macronematous conidiophores non-nodulose or only occasionally subnodulose due to geniculate proliferation; conidiogenous loci not
conined to swellings ........................................................................................................................................................................... 18
16 Conidia solitary or in short unbranched or branched chains, 5−8(−9) μm wide; phytopathogenic, causing leaf spots on Colocasia
.......................................................................................................................................................................................... C. colocasiae
16* Conidia always catenate, usually in densely branched chains, (1.5−)2−4(−5) μm wide; saprobes occurring on numerous substrates
............................................................................................................................................................................................................. 17
17 Conidiophores up to 720 μm or even longer, always nodulose to nodose with conidiogenous loci restricted to swellings (on SNA and
in vivo; on PDA and OA conidiophores without swellings ................................................................................................ C. oxysporum
17* Conidiophores up to 310(−460) μm long, often subnodulose or nodulose with a head-like swollen apex and sometimes few additional
nodes on a lower level, but most conidiophores neither geniculate nor nodulose, loci often situated on swellings but not restricted to
them, in intercalary conidiogenous cells loci often sitting at about the same level round about the stalk, but not connected with swellings
as in C. oxysporum; on PDA and OA conidiophores darker, often with swellings ........................................................ C. tenuissimum
18(15)Conidiophores 4−7(−8) μm wide at the base, attenuated towards the apex, 3−4 μm wide, medium to dark brown, often with a foot-like
swollen base .................................................................................................................................................................. C. basiinlatum
18* Conidiophores different, narrower or not distinctly attenuated towards the apex, paler, not dark brown, without a foot-like swollen
base ..................................................................................................................................................................................................... 19
19 Conidiophores up to 100 μm long, rarely longer and secondary ramoconidia up to 20 μm long, occasionally longer ........................ 20
19* Conidiophores up to 330 μm, and secondary ramoconidia up to 30 μm long ..................................................................................... 27
5
28
the genuS Cladosporium
20
20*
21
Conidia in long unbranched or loosely, mostly dichotomously branched chains ................................................................................. 21
Conidia usually in densely branched chains both at the base of the chain and intercalary ................................................................ 24
Conidiophores (3−)3.5−4.5 μm wide; conidia 0−3-septate; forming subglobose or globose, dense pseudoparenchymatous
conglomerations of swollen hyphal cells ............................................................................................................................ C. hillianum
21* Conidiophores 2.5−3.5(−4) μm; conidia 0−1(−2)-septate; without pseudoparenchymatous conglomerations .................................... 22
22 Conidial chains labellate, characteristically spread in a fan-like manner, secondary ramoconidia 11−27 μm long, 0(−1)-septate
....................................................................................................................................................................................... C. labelliforme
22* Conidial chains not labellate, secondary ramoconidia 7−19(−23) μm, 0−1(−2)-septate .................................................................... 23
23 Conidial chains very long, with up to 18 conidia; small terminal conidia 5−9 × 2−2.5 μm; cladosporioid scar structure with dome and
rim not clearly visible using light microscopy ...................................................................................................... C. chalastosporoides
23* Conidial chains shorter, with up to 8(−14) conidia; small terminal conidia shorter and narrower, 2.5−5 × 1.5−2 μm; dome and rim clearly
visible ............................................................................................................................................................................ C. funiculosum
24(20) Conidiophores with monopodial rejuvenations having a single terminal rather inconspicuous annellation; conidia 1−3(−3.5) μm wide;
conidiogenous loci and hila 0.5−1.5(−1.8) μm diam ....................................................................................................... C. gamsianum
24* Conidiophores without monopodial rejuvenations; conidia 3−5(−6) μm; conidiogenous loci and hila 0.5−2 μm diam ........................ 25
25 Conidiophores mostly 1−3-septate; small terminal and intercalary conidia 2−3 μm wide, secondary ramoconidia 3−4 μm wide;
conidiogenous loci and hila 0.8−1.8(−2) μm diam; on Myrtaceae ............................................................................... C. myrtacearum
25* Conidiophores pluriseptate; small terminal and intercalary conidia 2−4(−4.5) μm, secondary ramoconidia (2.5−)3−5(−6) μm;
conidiogenous loci and hila slightly wider; fungicolous or lichenicolous .............................................................................................. 26
26 Mycelium minutely verruculose to irregularly rough-walled; conidiophores 2.5−5(−6) μm wide; conidia smooth or almost so to inely
asperulate; fungicolous, occurring on chasmothecia of Phyllactinia ...................................................................... C. phyllactiniicola
26* Mycelium dimorphic, fertile hyphae irregularly rough-walled, sterile hyphae smooth; conidiophores narrower, 3−4 μm wide; conidia
smooth; on lichens ...................................................................................................................................................... C. licheniphilum
27(19) Conidia 1.5−3(−3.5) μm wide ............................................................................................................................................................. 28
27* Conidia up to 5(−7) μm, mostly 3−4 μm .............................................................................................................................................. 30
28 Conidiophores (8−)12−130(−150) μm long; conidia smooth or almost so to inely verruculose .................................. C. perangustum
28* Conidiophores longer, up to 330 μm long; conidia smooth or almost so ............................................................................................. 29
29 Conidiophores subulate, formed like an awl with a swollen base and distinctly attenuated towards the apex, 2−3 μm wide at the apex;
small terminal conidia 2.5−4.5(−5.5) × 2−2.5 μm .......................................................................................................... C. subuliforme
29* Conidiophores not subulate, somewhat wider and not distinctly attenuated towards the apex, (1.5−)2−4 μm wide; small terminal
conidia 3−6.5 × 1.5−2 μm ......................................................................................................................................... C. angustisporum
30(27) Conidia 0−3-septate ........................................................................................................................................................................... 31
30* Conidia 0−1-septate, rarely with an additional septum ........................................................................................................................ 35
31 Conidiophores (2−)3−4(−5) μm wide; conidia smooth to sometimes asperulate or minutely verruculose .......................................... 32
31* Conidiophores (2.5−)3−6(−6.5) μm; conidia smooth or almost so ...................................................................................................... 33
32 Conidia 3−5 μm wide, 0−3-septate, septa often darkened; mycelium dimorphic ............................................ C. paracladosporioides
32* Conidia 2−3.5(−4) μm wide, 0−1(−3)-septate, septa not darkened; mycelium not dimorphic .................................................... C. exile
33(31) Ramoconidia 24−43 × 3−3.5 μm; conidia in long loosely branched chains, often dichotomously branched, up to 10(−14) conidia in
the terminal unbranched part of the chain, small terminal conidia 4−8(−10) μm long; phytopathogenic on Cucurbitaceae
.................................................................................................................................................................................... C. cucumerinum
33* Ramoconidia longer and wider; conidia in branched chains, branching in all directions, up to ive conidia in the terminal unbranched
part of the chain, small terminal conidia (2−)3.5−5 μm long ................................................................................................................ 34
34 Intercalary conidia (2−)2.5−3(−4) μm wide, secondary ramoconidia (2.5−)3−4(−5) μm wide; conidiogenous loci and hila 0.5−2(−2.5) μm
diam; attaining 50−70 mm diam after 14 d on PDA, MEA and OA; occurring on ascomycetes and fruiting bodies of different
basidiomycetous fungi ................................................................................................................................................ C. lycoperdinum
34* Intercalary conidia (2.5−)3−4(−4.5) μm, secondary ramoconidia (2.5−)3−6 μm; conidiogenous loci and hila 0.8−3 μm diam; slower
growing on all media, attaining 17−32 mm diam after 14 d; saprobic and possibly endophytic ........................................... C. varians
35(30) Small terminal conidia (2.5−)3−4 μm wide, usually globose or subglobose, secondary ramoconidia (3−)4−5(−6) μm wide
...................................................................................................................................................................................... C. globisporum
35* Small terminal conidia narrower, up to 3 μm wide, subglobose, obovoid, ovoid or limoniform, but not globose, secondary ramoconidia
usually narrower .................................................................................................................................................................................. 36
36 Macronematous conidiophores 4−5(−6) μm wide, erect or decumbent; fungicolous, occurring on species of Taphrina
...................................................................................................................................................................................... C. phyllophilum
36* Macronematous conidiophores narrower, usually 2.5−4 μm wide, usually erect, not decumbent; on different substrates (cladosporioides
s. lat. complex) .................................................................................................................................................................................... 37
37 Conidia inversely coloured with small terminal and intercalary conidia being slightly darker than secondary ramoconidia, ramoconidia
and conidiophores; small terminal conidia (3−)5−8.5 μm long ....................................................................................... C. inversicolor
37* Conidia not inversely coloured, small terminal and intercalary conidia paler or concolorous with secondary ramoconidia, ramoconidia
and conidiophores; small terminal conidia (2−)3−5(−6) μm ................................................................................................................ 38
38 Conidiophores usually with a head-like swollen apex and sometimes a few additional swellings on a lower level and/or conidiophores
slightly to often distinctly sympodially proliferating, growth or branching proceeding in an angle of 45° to almost 90°; in intercalary
conidiogenous cells loci sitting at about the same level round about the stalk, garland-like (tenuissimum s. lat.) .............................. 39
www.studiesinmycology.org
29
BenSch et al.
38*
Conidiophores different, without apical or intercalary swellings, at most subnodulose, growth not proceeding in an angle of 45° to
almost 90° ........................................................................................................................................................................................... 40
39 Conidiophores usually with a head-like swollen apex, uni- or multilateral, and sometimes with few additional nodules on a lower level;
ramoconidia 22−41 μm long; conidia smooth, occasionally irregularly rough-walled ................................................... C. tenuissimum
39* Conidiophores without head-like swollen apex; ramoconidia 16−56 μm; the outer walls of small terminal conidia and intercalary conidia
often seem to detach, irregular, somewhat refractive ......................................................................................................... C. rectoides
40(38) Ramoconidia 3−5 μm wide; secondary ramoconidia 10−30(−38) μm long (av. approx. 19−21), small terminal conidia in long unbranched
chains, up to 10 conidia in the terminal part of the chain .................................................................................................................... 41
40* Ramoconidia up to 4 μm wide; secondary ramoconidia shorter, 7−25 μm long (av. approx. 15−16), occasionally few conidia longer,
terminal conidial chains shorter, up to six, mainly up to four conidia in the terminal unbranched part of the chain ............................ 42
41 Secondary ramoconidia 0−1(−2)-septate, intercalary conidia subrostrate or rostrate ......................................................... C. iranicum
41* Secondary ramoconidia usually aseptate, occasionally 1-septate, intercalary conidia not rostrate ....................... C. cladosporioides
[including morphologically indistinguishable but phylogenetically distinct lineages]
42(40) Conidiogenous loci and hila 0.5−1.5(−1.8) μm .......................................................................................... C. pseudocladosporioides
42* Conidiogenous loci and hila somewhat wider, 0.5−2 μm ..................................................................................................................... 43
43 Conidia almost smooth to often asperulate, loosely verruculose or irregularly rough-walled, especially in small terminal and intercalary
conidia ................................................................................................................................................................................................. 44
43* Conidia smooth or almost so ............................................................................................................................................................... 45
44 Intercalary conidia and secondary ramoconidia with numerous distal hila crowded at the apex, in intercalary conidia with 2−4(−6) hila,
in secondary ramoconidia with up to 6(−9) hila at the apex, small terminal conidia 2−4 μm long (av. 3.5), intercalary conidia 5−12 μm
long (av. 7.9), aseptate .................................................................................................................................................... C. xylophilum
44* Intercalary conidia and secondary ramoconidia with only few distal hila, in intercalary conidia with 1−2(−3) hila, in secondary conidia
with up to three hila, small terminal conidia 3.5−5(−5.5) μm (av. 4.4), intercalary conidia (4−)5−8(−9) μm long (av. 6.3), 0−1-septate
.................................................................................................................................................................................................... C. exile
45(43) Mycelium often forming dense ropes, hyphae 1−5 μm wide; conidiophores macronematous, often very long, up to 285 μm; due to the
special cell structure conidiophores and conidia often with disto-septa ........................................................................ C. australiense
45* Mycelium not forming ropes, hyphae (0.5–)1−3(−4) μm; conidiophores macronematous 50−165 μm long, micronematous
19−75(−100) μm long; conidiophores and conidia without disto-septa .......................................................................... C. delicatulum
Key to biotrophic and common saprobic foliicolous Cladosporium species in vivo based on morphology
and ecology
Beside the foliicolous Cladosporium species, this key contains some common, widespread saprobic species often occurring as secondary
invaders on living or fading leaves, i.e., on lesions caused by other fungi. Descriptions of surface ornamentations, e.g., smooth, verruculose,
etc., refer to light microscopy if not otherwise stated.
1
1*
2
2*
3
3*
4
4*
5
5*
6
6*
7
7*
8
8*
30
Conidia solitary, occasionally few conidia in very short un ..branched chains, 20–75 × 5–10 μm, 1–6-septate, smooth or almost so; on
Ranunculus ............................................................................................................................................................................... C. ferox
Conidia solitary or in short unbranched chains, but distinctly verruculose to verrucose or echinulate, or conidia in long, often branched
chains, if conidia smooth, much shorter, with few septa ....................................................................................................................... 2
Conidia 13–30(–45) × 7–12 μm, very thick-walled, distinctly 2–3-layered; conidiophores 120–300(–400) μm long; on Corypha
............................................................................................................................................................................................ C. coryphae
Conidial wall one-layered or conidiophores much shorter or conidia narrower ..................................................................................... 3
Stromata well-developed, 20–100 μm diam, later expanded, up to 500 μm diam, pustulate; conidiophores 100–250 μm long, straight
to geniculate-subnodulose; conidia 8–25 × 5–10 μm, 0–3-septate, coarsely and distantly verrucose, verrucae up to 1 μm diam,
distance up to 2.5 μm; on dead stems of Zinnia ............................................................................................................... C. oblongum
Stromata lacking or much smaller, or conidiophores shorter, and/or conidia smooth or with distinct ornamentation of the surface ..... 4
Conidia solitary or only some conidia in short, mostly unbranched chains, very broad, 6–25 μm, average ≥ 8 μm (conidia sometimes
dimorphic, broad conidia mixed with some narrower ones) .................................................................................................................. 5
Conidia solitary, but narrower, less than 8 μm wide, or conidia in long, often branched chains .......................................................... 24
Conidiophores strongly geniculate-sinuous, terminally often coralloid, with distinct protuberances or denticulate, 20–145 × 4.5–8 μm;
conidia solitary or in short unbranched, rarely branched chains, 9–35 × (8.5–)10–15 μm, 0–1(–2)-septate; on Melosperma
..................................................................................................................................................................................... C. melospermae
Conidiophores distinctly torulose-nodulose to geniculate-nodulose or non- to only slightly geniculate, but not coralloid ..................... 6
Conidiophores distinctly torulose-nodulose or geniculate-nodulose, often with constrictions at the septa ........................................... 7
Conidiophores straight to somewhat geniculate-sinuous, but neither torulose-nodulose nor geniculate-nodulose ............................ 16
Conidia 40–65 × (15–)18–25 μm, strongly constricted at the septa; on Polygonatum ..................................................... C. polygonati
Conidia narrower, on the average less than 20 μm and/or not constricted at the septa ........................................................................ 8
Conidia dimorphic, large conidia formed singly or only in very short, unbranched chains, 8–18 μm wide, mixed with narrower catenate
conidia, 5–8 μm wide ............................................................................................................................................................................. 9
Conidia uniformly large, above all wide ............................................................................................................................................... 10
the genuS Cladosporium
9
9*
10(8)
10*
11
11*
12
12*
Large conidia 22–45 × 8.5–18 μm; biotrophic on Robinia .................................................................................................... C. robiniae
Large conidia in vivo up to 30 × 8–12(–15) μm wide; saprobic .................................................................................. C. macrocarpum
Conidia (10–)15–30 × 10–14 μm, (0–)1–septate; on Brassica .......................................................................................... C. brassicae
Conidia larger, above all longer, or pluriseptate .................................................................................................................................. 11
In vitro with twisted aerial hyphae; conidia in vivo 8–11(–12) μm wide; on Spinacia ........................................................... C. variabile
Without twisted aerial hyphae in vitro; conidia wider ........................................................................................................................... 12
Conidia ellipsoid-cylindrical, usually somewhat attenuated towards the base, short obconically truncate .......................................... 13
At least some conidia laterally gradually narrowed, solei-form (sole-shaped), sometimes distinctly bulbose at the base which is broadly
rounded, i.e., not attenuated ............................................................................................................................................................... 14
13 Conidia (15–)20–55(–60) × 9–15(–17) μm, (0–)1–3(–4)-septate, often constricted at the primary septum, wall coarsely verrucosepustulate; on Narthecium ..................................................................................................................................................... C. lacroixii
13* Conidia up to 70 × 15 μm, with up to six septa, without any constrictions, wall verrucose-digitate; on Gagea and Ornithogalum
......................................................................................................................................................................................... C. ornithogali
14(12) Conidia about 25–55 × 12–15 μm, (1–)2–4(–6)-septate; conidiophores often with small side branches; on Dianthus and possibly other
members of the Caryophyllaceae .................................................................................................................................. C. echinulatum
14* Conidia only 0–3-septate or larger, up to 75 × 20 μm; conidiophores without any side branches; on Iris ........................................... 15
15 Conidia 30–75 × 10–20(–25) μm, (0–)2–6(–7)-septate, wall up to 1 μm wide .......................................................................... C. iridis
15* Conidia 15–55 × (9–)11–19(–21) μm, 0–3-septate, wall thicker, up to 2 μm, usually somewhat zonate ......................... C. pseudiridis
16(6) Conidiophores uniformly short, 8–40 × 4–10 μm; conidia 15–30 × 8–13 μm, 0–2-septate; on Haplophyllum ................ C. haplophylli
16* Conidiophores much longer, on the average > 40 μm ......................................................................................................................... 17
17
Conidia (11–)20–60 × (5–)7–12 μm; conidiophores fasciculate, arising from substomatal stromata, 20–50 μm diam; on Arthropodium
......................................................................................................................................................................................... C. arthropodii
17* Conidia shorter or wider, or substomatal stromata lacking .................................................................................................................. 18
18 At least some conidia gradually narrowed in the middle, solei-form, at the base often somewhat swollen, broadly rounded; on Allium
............................................................................................................................................................................................................. 19
18* Conidia subglobose, subclavate, ellipsoid-ovoid to short cylindrical, base not swollen, but somewhat attenuated ............................ 20
19 Conidia (20–)30–60(–80) μm long; conidiogenous loci protuberant, ‘peg-like’; on various Allium spp. ....................................... C. allii
19* Conidia longer, (40–)60–90(–120) μm; conidiogenous loci non-protuberant, ≤ 1 μm high, not ‘peg-like’; on Allium cepa and A. istulosa
........................................................................................................................................................................................... C. allii-cepae
20(18) Conidia in vivo (10–)15–30(–35) × (6–)7–9(–11) μm, width on the average < 9 μm, in vitro even narrower; on Narthecium
............................................................................................................................................................................................. C. ossifragi
20* Conidia in vivo wider, 6–14 μm, on the average > 9 μm ...................................................................................................................... 21
21 Conidia in short, unbranched, rarely branched chains ........................................................................................................................ 22
21* All or almost all conidia formed singly ................................................................................................................................................. 23
22 Conidia (0–)1–5-septate; on Trillium .......................................................................................................................................... C. trillii
22* Conidia 0–1(–2)-septate; on Agoseris .............................................................................................................................. C. agoseridis
23(21) Conidia 0–1-septate, base somewhat attenuated; on Alopecurus .................................................................................... C. alopecuri
23* Conidia 0–3-septate, base broadly rounded; on Phleum ........................................................................................................... C. phlei
24(4) Conidiophores dimorphic, forming two types different in shape, size, septation, pigmentation and sometimes thickness of the wall
............................................................................................................................................................................................................. 25
24* Conidiophores uniform, not distinctly dimorphic .................................................................................................................................. 28
25 Conidiophores up to 300 μm long, protoplasm of the conidiophore cells often conspicuously aggregated at the septa forming a paler
cavity (lumen) in the centre of the cells; conidia usually 0–1-septate; on Eriobotrya .............................................. C. fusicladiiformis
25* Conidiophores usually shorter, protoplasm of the cells not aggregated at the septa; conidia 0–2(–6)-septate ...................................26
26 Conidiophores 3–6 μm wide, often distinctly nodulose; conidia 3–27 × 3–6.5 μm, 0–6-septate; on Dieffenbachia, probably saprobic
................................................................................................................................................................................... C. dieffenbachiae
26* Conidiophores 3–8 μm wide, subnodulose or slightly geniculate-sinuous, but not distinctly nodulose; conidia shorter and somewhat
narrower, 2.5–19(–28) × 2–5(–6.5) μm, 0–2(–5)-septate .....................................................................................................................27
27 Conidiophores with numerous crowded conidiogenous loci, apex of conidiogenous cells appearing somewhat rugose, periconiellalike, walls of the conidiophores thickened, up to 1(–1.25) μm wide, smooth, cells often with distinct clearly delineated lumen; ramoconidia
occasionally occurring; on Syringa .................................................................................................................................. C. syringicola
27* Apex of conidiogenous cells not periconiella-like, walls of conidiophores up to 2 μm wide, with age outer wall seemingly detaching
irregularly, cells without distinct lumen; no ramoconidia; on Chrysophyllum ..................................................................C. chrysophylli
28(24) Conidiophores distinctly nodulose or nodose, conidiogenous loci usually conined to swellings ....................................................... 29
28* Conidiophores non-nodulose or occasionally only subnodulose due to geniculate proliferations, but conidiogenous loci not conined to
swellings, or only tips of the conidiophores somewhat swollen ........................................................................................................... 35
29 Conidia usually smooth or almost so ................................................................................................................................................... 30
29* Conidia with different surface ornamentation, minutely to distinctly verruculose or verrucose, asperulate or irregularly rough-walled
............................................................................................................................................................................................................. 32
30 Conidiophores 40–105 μm long, nodulose, swellings not regular, neither clearly separated nor distant from each other, in quick
succession; on Borassus ....................................................................................................................................................... C. borassi
30* Conidiophores longer, up to 250 μm long or even longer, occasionally up to 500 μm long, straight, nodose with distinct, regular
www.studiesinmycology.org
31
BenSch et al.
swellings, clearly separated and distant from each other .................................................................................................................... 31
Conidia 0–1(–2)-septate, subglobose, ovoid, limoniform or ellipsoid, 3–6 μm wide; saprobic, on numerous substrates, also as a
secondary invader on leaf spots caused by other fungi .................................................................................................. C. oxysporum
31* Conidia 0–3(–5)-septate, broadly ellipsoid-subcylindrical to cylindrical, wider, (5–)6–9(–10) μm; on Colocasia .............. C. colocasiae
32(29) Conidiophores unbranched to often branched; conidia almost smooth to minutely asperulate or irregularly rough-walled, walls
sometimes even two-layered, with unusual cell structure (cells with a paler cavity in the centre of the cells); on Ocotea
..................................................................................................................................................................................... C. oreodaphnes
32* Conidiophores mostly unbranched, rarely branched; conidia usually minutely to distinctly verruculose or verrucose, walls usually not
two-layered, without unusual cell structure ......................................................................................................................................... 33
33 Conidiophores often with a single head-like, terminal swelling; conidia (2.5–)3–6.5 μm wide, 0–2(–3)-septate, usually minutely
verruculose, occasionally smooth; on Galium ............................................................................................................................. C. galii
33* Conidiophores with several or numerous intercalar and terminal swellings; conidia usually wider, up to 12(–15) μm, with up to ive
septa, usually distinctly verruculose to verrucose ............................................................................................................................... 34
34 Conidia (5–)11–36(–41) μm long, (0–)1–4(–5)-septate; on Trillium ............................................................................................ C. trillii
34* Conidia much shorter, mostly up to 25 μm long and 0–3-septate; saprobic, on numerous substrates, also as a secondary invader on
leaf spots caused by other fungi ................................................................................................................................. C. macrocarpum
35(28) Conidiophores usually in small to moderately large, loose to dense fascicles, usually arising from stromata, stromatic hyphal
aggregations or swollen hyphal cells, emerging through stomata or erumpent through the cuticle, occasionally solitary ...................36
35* Conidiophores solitary or occasionally in loose groups but not fasciculate, usually arising from hyphae or swollen hyphal cells ...... 75
36 Conidia smooth or almost so, rarely few conidia faintly rough-walled ................................................................................................. 37
36* Conidia minutely to distinctly verruculose-echinulate, rarely few conidia smooth ............................................................................... 53
37 Conidiophores up to 100 μm long, but usually much shorter .............................................................................................................. 38
37* Conidiophores usually longer than 100 μm ......................................................................................................................................... 49
38 Conidia 10–45(–68) μm long, occasionally up to 180 μm in length, 0–4(–7)-septate; conidiogenous loci and hila (1–)1.5–3(–4) diam;
on Allium ........................................................................................................................................................................... C. victorialis
38* Conidia usually much shorter, with up to three septa; conidiogenous loci and hila usually narrower ................................................. 39
39 Conidia 0–1-septate, rarely with two septa ......................................................................................................................................... 40
39* Conidia 0–3(–4)-septate ...................................................................................................................................................................... 44
40 Conidiophores often branched ............................................................................................................................................................ 41
40* Conidiophores usually unbranched ..................................................................................................................................................... 42
41 Conidia often distinctly constricted at the septa; on Corymbia .................................................................................... C. myrtacearum
41* Conidia not constricted at the septa; on Cycas ..................................................................................................................... C. cycadis
42(40) Conidia up to 25 μm long and 7 μm wide; on Passilora ................................................................................................... C. maracuja
42* Conidia shorter, up to 18 μm long, 2–5(–6) μm wide ........................................................................................................................... 43
43 Conidiophores fasciculate as well as solitary, arising from internal and external hyphae, often with percurrent, enteroblastic
proliferations; conidiogenous loci and hila very small, up to 1 μm diam; ramoconidia sometimes occurring; conidia up to 11 μm long;
on Cassia and Chamaecrista .......................................................................................................................... C. cassiae-surathensis
43* Conidiophores usually fasciculate, arising from stromata or stromatic hyphal aggregations, mycelium not external, conidiophores
without enteroblastic proliferations; conidiogenous loci and hila 0.5–1.5(–2) μm diam; ramoconidia absent; conidia somewhat longer,
1.5–18 μm; on Rhododendron ...................................................................................................................................... C. rhododendri
44(39) Conidia (3–)4–18(–20) μm long ......................................................................................................................................................... 45
44* Conidia longer, up to 30 μm ................................................................................................................................................................ 48
45 Conidiophores fasciculate as well as solitary, arising from internal and external hyphae; ramoconidia occasionally occurring; conidia
2–14(–16) μm long; on Capparis ...................................................................................................................................... C. lineolatum
45* Conidiophores usually fasciculate; mycelium not external; ramoconidia absent; conidia somewhat longer ....................................... 46
46 Conidiophores erect to decumbent, unbranched or often branched; on Manihot .......................................................... C. nigrelloides
46* Conidiophores always erect, not decumbent, usually unbranched, rarely branched .......................................................................... 47
47 Conidiophores 10–58 × 3.5–5 μm, often distinctly paler at the apex, almost hyaline; on Jacaranda .......................... C. jacarandicola
47* Conidiophores 15–70 × 3–6 μm, not distinctly paler at the apex; on Citrus ................................................................... C. corrugatum
48(44) Conidiophores erect to decumbent, fasciculate or solitary, arising from creeping hyphae, also on trichomes; conidia (8–)10–30(–35) ×
2.5–6 μm; on Gynoxys ....................................................................................................................................................... C. inopinum
48* Conidiophores always erect, arising from stromatic hyphal aggregations, not from creeping hyphae, not on trichomes; conidia
somewhat wider, 6–28 × 4–8 μm; on Lupinus ............................................................................................................... C. lupiniphilum
49(37) Conidiophores 2.5–5 μm wide; conidia smooth to often minutely verruculose; on Smilax ............................................. C. smilacicola
49* Conidiophores usually much wider, (3–)4–11(–16) μm; conidia usually smooth under light microscopy ............................................ 50
50 Conidia (0–)1–6-septate, 9–45 × 4–8 μm; on Photinia .................................................................................................... C. diaphanum
50* Conidia with up to three septa, up to 20 μm long ................................................................................................................................ 51
51 Ramoconidia present, truncate at the base, 2–4 μm wide; conidiophores 50–300 × 3–8 μm; saprobic on leaves of Magnolia
............................................................................................................................................................................................ C. delectum
51* Ramoconidia lacking ........................................................................................................................................................................... 52
52 Conidiophores 50–175 × (4–)5–8(–11) μm, walls thickened, (0.5–)1–2 μm wide, cells often with a distinct small inner lumen clearly
separated from the inner wall; conidia 4–14 μm long, 0–1(–2)-septate, smooth or almost so, but mostly minutely verruculose when
31
32
the genuS Cladosporium
viewed by SEM; on Populus ............................................................................................................................................. C. populicola
Conidiophores (23–)150–360 μm or even longer, 6–11(–16) μm wide at the base, distinctly attenuated towards the apex, (2–)3–5.5
μm wide, walls thickened, 0.75–3 μm wide, protoplasm of the cells somewhat aggregated at the septa; conidia 2–22 μm long,
0–3-septate, smooth, with an unusual cell structure (with paler cavity in the centre of the cells); on Cycas ......................... C. apicale
53(36) Conidiophores up to 100(–120) μm long, but usually much shorter ................................................................................................... 54
53* Conidiophores usually longer than 100 μm ......................................................................................................................................... 67
54 Conidia solitary or in simple chains; stromata large, at irst 10–60 μm diam, later enlarged or conluent, up to 150 μm diam; on legumes
of Phaseolus ................................................................................................................................................................. C. leguminicola
54* Conidia at least partly in branched chains; stromata smaller .............................................................................................................. 55
55 Conidiophores up to 10 μm wide, distinctly rough-walled; hyphae up to 13 μm wide ......................................................................... 56
55* Conidiophores narrower, up to 7 μm wide (at the very base occasionally wider), usually smooth; hyphae much narrower ............... 57
56 Conidiophores 10–60 μm long; conidia without distinct small central lumen; on Aesculus .................................... C. asperistipitatum
56* Conidiophores up to 120(–180) μm long; conidia often with distinct visible central lumen; on Gentiana .......................... C. gentianae
57(55) Conidia up to 22 μm long ................................................................................................................................................................... 58
57* Conidia usually longer, up to 30 μm long ............................................................................................................................................ 64
58 Conidiogenous loci and hila small, usually 0.5–1.5 μm diam .............................................................................................................. 59
58* Conidiogenous loci and hila wider, 1–2.5 μm diam ............................................................................................................................. 61
59 Conidiophores only slightly geniculate-sinuous; conidia without distinct lumen; on Rhododendron ............................. C. rhododendri
59* Conidiophores usually distinctly geniculate-sinuous or sub-nodulose; conidia often with distinct lumen ............................................ 60
60 On living leaves, but without leaf spots; conidia 0–1(–3)-septate; on Salix ................................................................... C. minusculum
60* Forming distinct brown leaf spots; conidia 0–3(–4)-septate; on Eriobotrya .................................................................... C. eriobotryae
61(58) Conidiophores few to often numerous, in small to moderately large, loose to dense fascicles ......................................................... 62
61* Conidiophores solitary or few in loose fascicles .................................................................................................................................. 63
62 Conidiophores up to 125 μm long, 4–6.5 μm wide, usually unbranched without distinct constrictions; conidia 2–5(–6.5) μm wide; on
Chamaerops ................................................................................................................................................................. C. chamaeropis
62* Conidiophores up to 80 μm long, 3–8 μm wide, often branched with distinct constrictions at the septa; conidia 3–7 μm wide; on Yucca
................................................................................................................................................................................................ C. yuccae
63 Conidiophores up to 75 μm long; conidia ellipsoid-ovoid to cylindrical, ends rounded or only slightly attenuated, conidia 0–3-septate,
usually distinctly verruculose; on Oncidium .................................................................................................................. C. orchidearum
63* Conidiophores 10–40 μm long; catenate conidia distinctly fusiform, with attenuated, pointed ends, 0–1-septate, almost smooth to
verruculose; on Ruta ................................................................................................................................................................. C. rutae
64(57) Hyphae radiating, forming loose to dense stromatic hyphal plates (fusicladium-like growth), hyphal cells sometimes irregularly lobed;
conidiophores unbranched or once branched; on Angelica ................................................................................................. C. foliorum
64* Hyphae not radiating, without fusicladium-like growth, hyphal cells not irregularly lobed; conidiophores unbranched, only very rarely
branched ............................................................................................................................................................................................. 65
65 Conidiogenous loci and hila 1–1.5 μm diam; conidia 2–5 μm wide, 0–1-septate, almost smooth to verruculose; on Ilex ... C. cheonis
65* Conidiogenous loci and hila (1–)1.5–2.5 μm diam; conidia (3–)4–8(–9) μm wide, 0–3(–4)-septate ................................................... 66
66 Conidiophores 8–54 μm long, 0–1(–2)-septate; conidia 0–1(–3)-septate, faintly to conspicuously verruculose-echinulate;
on Tragopogon ..................................................................................................................................................................... C. praecox
66* Conidiophores up to 100 μm long, 0–4(–6)-septate; conidia 0–3(–4)-septate, verruculose; on Vinca ................................ C. vincicola
67(53) Conidiophores in large, dense fascicles, at the base of the conidiomata more or less parallel, synnema-like; stromata large, 40–150
μm wide, sometimes conluent and even larger, several layers deep; on Soldanella .................................................... C. soldanellae
67* Conidiophores in small to moderately large, loose to dense fascicles, but conidiomata not synnema-like; stromata smaller ............ 67
68 Conidiophores 2.5–7 μm wide ............................................................................................................................................................. 69
68* Conidiophores usually wider, at least up to 10 μm .............................................................................................................................. 73
69 Conidia in vivo (6–)7–9(–11) μm wide (in vitro 5–8 μm); on Narthecium ............................................................................. C. ossifragi
69* Conidia in vivo narrower, 2.5–6(–7) μm ............................................................................................................................................... 70
70 Conidiophores often with percurrent, enteroblastic proliferations; conidiogenous cells with numerous, often crowded conidiogenous
loci, loci and hila small, 1–1.5 μm diam; conidia 3–15(–19) μm long, with an unusual cell structure (with distinct, central paler lumen in
the centre of the cells); on Cypripedium ..................................................................................................................... C. orchidiphilum
70* Conidiophores only sporadically with enteroblastic proliferations; conidiogenous cells with a single or several conidiogenous loci,
neither numerous nor crowded, loci and hila usually somewhat wider, 1–2(–2.5) μm diam; conidia usually longer, without an unusual
cell structure ........................................................................................................................................................................................ 71
71 Conidiophores usually unbranched; conidiogenous loci arranged on about the same level, like a garland; conidia usually 0–4(–5)-septate;
on Stanhopea ................................................................................................................................................................. C. stanhopeae
71* Conidiophores unbranched or often branched, once or several times; conidiogenous loci not arranged like a garland; conidia usually
0–3-septate ......................................................................................................................................................................................... 72
72 Conidia 3.5–26 × 3–7(–8) μm, with age becoming longer and wider, distinctly swollen, up to 36 μm long or even longer and up to 11
μm wide, with up to seven septa, forming secondary conidiophores; without ramoconidia; on Dracaena and Cordyline
...................................................................................................................................................................................... C. dracaenatum
72* Conidia shorter and narrower, 2–18(–21) × 1.5–5(–6) μm, with age neither longer and wider nor swollen; ramoconidia occasionally
occurring, up to 26 μm long, 0(–1)-septate; on Smilax .................................................................................................... C. smilacicola
52*
www.studiesinmycology.org
33
BenSch et al.
73(68) Conidiophores up to 310 μm long or even longer, 5–15 μm wide near the base, distinctly attenuated towards the apex, 3–5(–6) μm
wide; conidia with an unusual cell structure (with paler lumen in the centre of the cells); hyphae 2–4 μm wide; on Berberis
..................................................................................................................................................................................... C. ushuwaiense
73* Conidiophores shorter, up to 180 μm long, not distinctly attenuated towards the apex, up to 10 μm wide; conidial cells without small
lumen in the centre, but lumen often distinct, clearly separated from the inner wall; hyphae up to 11 μm wide or even wider ........... 74
74 Conidia 3.5–23 × 3–6(–8) μm, almost smooth to often verruculose or irregularly rough-walled; on Gentiana .................. C. gentianae
74* Conidia 4–14 × 3–5(–5.5) μm, smooth or almost so to slightly verruculose (light microscopy), but most conidia minutely verruculose
when viewed by SEM; on Populus ................................................................................................................................... C. populicola
75(35) Conidiophores characteristically branched, right-angled; on Epidendrum .................................................................. C. rectangulare
75* Conidiophores unbranched or branched, but not right-angled ............................................................................................................ 76
76 Conidiophores always with a conspicuously swollen bulbous base, up to 16 μm wide; on Rivina ......................................... C. rivinae
76* Base of conidiophores not distinctly swollen, not bulbous .................................................................................................................. 77
77 Mycelium external; conidiophores consistently arising from supericially growing hyphae ................................................................. 78
77* Mycelium consistently internal or sometimes both internal and external; conidiophores usually arising from swollen hyphal cells or
internal hyphae, occasionally also from external creeping hyphae ..................................................................................................... 93
78 Conidiophores very long, 15–500 μm, often dichotomously branched, tips (conidiogenous cells) often somewhat swollen; probably
saprobic ................................................................................................................................................................................. C. varians
78* Conidiophores either much shorter or unbranched to irregularly branched ........................................................................................ 79
79 Web-like mycelial colonies or patches on green leaves attacked by aphids or only with honey dew, without any lesions, formed by
germinated conidia; mycelium without any nutritive contact to the host leaves, i.e., completely supericial; hyphae 2–6 μm wide,
but often with swollen cells, up to 10 μm diam, monilioid, conidiophores 10–120(–300) × 3–8.5 μm; conidia 3–20 × 2–5 μm; secondary
ramoconidia 8–25 × 3–8.5 μm, 0–3-septate, cells often with a distinct central lumen .......................................................... C. aphidis
79* Either biotrophic species, causing lesion, or saprobic species ........................................................................................................... 80
80 Conidiophores usually 2–6 μm wide .................................................................................................................................................... 81
80* Conidiophores usually wider, 3–9(–11) μm .......................................................................................................................................... 89
81 Conidia narrow, usually 2–5 μm wide, on the average < 5 μm ............................................................................................................ 82
81* Conidia usually wider, up to 8 μm ........................................................................................................................................................ 86
82 Conidiogenous loci and hila small, 0.5–1.5 μm diam .......................................................................................................................... 82
82* Conidiogenous loci and hila wider, up to 2.5 μm diam ........................................................................................................................ 85
83 Conidiophores erect to decumbent; conidia 3–23(–27) μm long, 0–3(–4)-septate; on Heterophragma ............... C. heterophragmatis
83* Conidiophores always erect; conidia usually shorter, usually 0–1-septate, only occasionally with up to three septa sometimes with an
unusual cell structure .......................................................................................................................................................................... 84
84 Mycelium smooth to mostly more or less verruculose or irregularly rough-walled; conidia 4–17(–19) μm long, with age becoming
distinctly enlarged, longer and wider, 5–7 μm wide, lumen often distinct, clearly separated from the inner wall; on Ulmus
...................................................................................................................................................................................... C. hypophyllum
84* Mycelium smooth; conidia 4–14(–20) μm long, not distinctly enlarged with age, lumen of the cells not distinct; on Styrax
.................................................................................................................................................................................... C. inconspicuum
85(82) Conidiophores 0–3-septate; conidia 0–2-septate; on Mimulus ...................................................................................... C. mimulicola
85* Conidiophores pluriseptate, with up to 11 septa; conidia 0–4(–5)-septate; on Nerium ....................................................... C. neriicola
86(81) Conidiophores 5–50 μm long, 0–3-septate; conidia 5–8 μm wide, 0–5-septate, often 3-septate; on Artemisia ............... C. obtectum
86* Conidiophores 20–200(–250) μm, pluriseptate; conidia (2.5–)3–6.5 μm wide .................................................................................... 87
87 Conidia usually 0–1(–2)-septate, 2–18 μm long, only with age becoming longer and more frequently septate, with up to four septa, with
paler reduced lumen in the centre of the cells; on Bougainvillea ................................................................................. C. arthrinioides
87* Conidia 0–4(–5)-septate, 5–35(–55) μm long, cells without distinct lumen ......................................................................................... 88
88 Conidiophores and conidia verruculose; on Euonymus .............................................................................................. C. subobtectum
88* Conidiophores and conidia smooth; on Caragana ........................................................................................................... C. caraganae
89(80) Conidiophores 5–50 μm long, 3–5(–7) μm wide, 0–3-septate; on Artemisia .................................................................... C. obtectum
89* Conidiophores much longer, pluriseptate ............................................................................................................................................ 90
90 Conidiophores often several times mildly to distinctly geniculate-sinuous, walls often distinctly two-layered; ramoconidia frequently
formed, 17–60 × 4.5–8 μm, 0–3(–5)-septate; on Ulmus and other hosts, probably saprobic ............................................... C. varians
90* Conidiophores usually not geniculate-sinuous, walls more or less thickened, but not distinctly two-layered; ramoconidia absent .... 91
91 Stromata (external) composed of more or less isodiametrical cells, forming a textura angularis; conidiophores 15–300 × 2.5–8 μm,
arising solitarily from creeping hyphae or in loose groups from stromata; conidia 0–2(–5)-septate, walls unthickened or almost so;
on Piper ............................................................................................................................................................................. C. pipericola
91* Stromata lacking .................................................................................................................................................................................. 92
92 Conidiophores 5–150(–210) × (2–)3–6 μm, solitary, often also formed secondarily (microcyclic conidiogenesis); conidia 0–1(–3)-septate,
with age becoming distinctly swollen, longer and wider, up to 12 μm wide or even wider, pluriseptate, often constricted at the septa
and thick-walled, sometimes even two-layered; hyphae, conidiophores and conidia sometimes with paler lumen in the centre of the
cells; on Heliotropium ........................................................................................................................................................ C. heliotropii
92* Conidiophores 20–300 μm long, 2.5–5 μm wide above and 4–10 μm wide below, wall 0.5–1 μm thick, one-layered; conidia
0–3(–5)-septate, unchanged with age, thin-walled; conidiophores and conidia without paler lumen; on pods of Butea .... C. buteicola
93(77) Conidiophores narrow, usually 2–5 μm wide ...................................................................................................................................... 94
34
the genuS Cladosporium
93*
94
Conidiophores usually somewhat wider, 3–8(–11) μm ...................................................................................................................... 102
Conidia 0–4(–7)-septate, commonly 3-septate, septa usually thickened and distinctly darkened; on Cenchrus and Chaetochloa
....................................................................................................................................................................................... C. spongiosum
94* Conidia with up to three septa, but septa neither conspicuously thickened nor darkened .................................................................. 95
95 Conidiophores solitary, arising from internal and external hyphae or in small loose fascicles ............................................................ 96
95* Conidiophores always solitary, non-fasciculate ................................................................................................................................. 100
96 Conidia usually 0–1-septate ................................................................................................................................................................ 97
96* Conidia 0–3-septate ............................................................................................................................................................................ 99
97 Conidiophores 20–100 μm long, often with percurrent, enteroblastic proliferations; conidiogenous loci and hila very small, up to 1 μm
diam; conidia 3–11 × 2–4 μm; on Cassia and Chamaecrista ........................................................................... C. cassiae-surathensis
97* Conidiophores up to 250 μm long, without percurrent, enteroblastic proliferations; conidiogenous loci and hila 0.5–2.5 μm diam;
conidia 3–30 × 2–5(–7) μm .................................................................................................................................................................. 98
98 Conidiophores often distinctly geniculate-sinuous with numerous densely aggregated conidiogenous loci, conidiogenous cells both
terminal and intercalary; on Pisum ........................................................................................................................................ C. pisicola
98* Conidiophores iliform or subcylindrical, neither distinctly geniculate-sinuous nor nodulose with 1–3, rarely more conidiogenous loci;
conidiogenous cells usually terminal; saprobic ...................................................................................................... C. cladosporioides
99(96) Conidiogenous loci and hila 0.5–1.5(–2) μm diam; conidia 2–4.5 μm wide; on Capparis ............................................... C. lineolatum
99* Conidiogenous loci and hila 1–2 μm diam; conidia 3.5–8.5 μm wide ............................................................................ C. gleditschiae
100(95) Conidiophores 14–150 μm long, unbranched or once branched, arising from internal and external, creeping hyphae; without
microcyclic conidiogenesis; on Quercus ....................................................................................................................... C. fumagineum
100* Conidiophores usually unbranched, always arising from internal hyphae or swollen hyphal cells, longer, up to 275 μm ................. 101
101 Microcyclic conidiogenesis often occurring; conidia 3.5–23(–31) × 2–5(–7) μm, 0–2(–3)-septate, pale, hyaline, subhyaline to very pale
brown; on Xyris ....................................................................................................................................................................... C. xyridis
101* Microcyclic conidiogenesis lacking; conidia shorter and wider, 8–14 × 4–8 μm, usually aseptate; on Carpesium .............. C. carpesii
102(93) Conidia 0–6(–8)-septate, conidial cells sometimes with paler lumen in the centre; on Boscia .......................................... C. bosciae
102* Conidia 0–4-septate, conidial cells without paler lumen in the centre ............................................................................................... 103
103 Ramoconidia often occurring, 15–23 × 5–7.5 μm, 0(–1)-septate; mycelium sometimes irregularly lobed; on Ranunculus
.................................................................................................................................................................................. C. grech-delicatae
103* Ramoconidia absent or only rarely formed; mycelium not irregularly lobed ...................................................................................... 104
104 Conidia 3–15(–20) μm long ............................................................................................................................................................... 105
104* Conidia up to 35 μm long or even longer .......................................................................................................................................... 107
105 Conidiophores (4–)5–8(–11) μm wide, often with percurrent, enteroblastic proliferations, walls (0.5–)1–2 μm wide, cells often with
distinct, small inner lumen clearly separated from the inner wall; conidia smooth or almost so to somewhat verruculose (when viewed
by SEM most conidia minutely verruculose), cells sometimes with distinct inner lumen; on Populus .............................. C. populicola
105* Conidiophores (3.5–)4–7 μm wide, usually without enteroblastic proliferations, walls up to 1 μm wide, cells without distinct inner
lumen, but protoplasm somewhat aggregated at the septa; conidia smooth, cells without distinct inner lumen ............................... 106
106 Conidia (2.5–)3.5–6(–7) μm wide; on Liriodendron .......................................................................................................... C. liriodendri
106* Conidia (2–)3–4.5(–6) μm wide; on Annona ........................................................................................................................ C. annonae
107(104) Immersed hyphae often with a slime coat; on Cucurbitaceae ............................................................................... C. cucumerinum
107* Hyphae without slime coat ................................................................................................................................................................ 108
108 Conidia 3.5–8.5(–9) μm wide ............................................................................................................................................................ 109
108* Conidia narrower, 2.5–6(–7) μm ........................................................................................................................................................ 110
109 Conidiophores often subnodulose, with unilateral swellings, but conidiogenous loci not conined to swellings; all structures with oil
droplets; on Arabis ............................................................................................................................................. C. subsclerotioideum
109* Conidiophores geniculate-sinuous, but non-nodulose; without oil droplets; on Gleditsia .............................................. C. gleditschiae
110(108) Conidiogenous cells almost consistently terminal, conidiophores non or barely geniculate; saprobic species ........................... 111
110* Conidiogenous cells terminal, but also intercalary; biotrophic leaf-spotting species ......................................................................... 113
111 All conidia distinctly verruculose to verrucose; mycelium internal and external; solitary conidiophores arising from supericial hyphae
present ............................................................................................................................................................................... C. auriculae
111* Conidia consistently smooth or smooth to faintly rough-walled ......................................................................................................... 112
112 Conidiophores very long and iliform-setiform, mostly 100–500 μm long, pluriseptate, pigmented, mostly medium to medium dark
brown or olivaceous-brown and thick-walled (up to 1.5 μm), tips often unilaterally swollen; conidia smooth to verruculose
...................................................................................................................................................................................... C. tenuissimum
112* Conidiophores up to about 250 μm long, mostly much shorter, paler and thin-walled, ≤ 1 μm, tips not swollen, at most sudenticulate;
conidia usually consistently smooth, only occasionally with a few faintly rough-walled conidia ............................. C. cladosporioides
113(110) Conidia 3–23(–25) μm long .......................................................................................................................................................... 114
113* Conidia longer, up to 30 μm long or even longer ............................................................................................................................... 115
114 Walls of the conidiophores 0.5–1 μm wide; on Alnus, Europe .............................................................................................. C. alneum
114* Walls of the conidiophores (0.5–)1–2 μm wide; on Oncoba, New Zealand ......................................................................... C. oncobae
115(113) Mycelium both internal and external ............................................................................................................................................. 116
115* Mycelium only internal; walls of the conidiophores mostly only slightly thickened, not two-layered; conidia mostly 0–1-septate, very
rarely with a second septum, usually smooth .................................................................................................................................... 117
www.studiesinmycology.org
35
BenSch et al.
116 Conidiophores with thickened walls, sometimes even two-layered; conidia 0–2(–3)-septate, smooth to minutely verruculose; on
Fraxinus ............................................................................................................................................................................ C. fraxinicola
116* Conidiophores thin-walled and one-layered; conidia 0–4(–5)-septate, smooth; on Caragana ......................................... C. caraganae
117 Ramoconidia occasionally formed; conidiogenous loci and hila 0.5–2 μm diam; on Lespedeza and Vigna ........................... C. vignae
117* Ramoconidia absent; conidiogenous loci and hila 1–3 μm diam; on Psoralea .................................................................. C. psoraleae
Tabular key to foliicolous Cladosporium species based on host families and genera
Several probably biotrophic Cladosporium species have recently been described from China by Z.Y. Zhang and co-workers (types deposited
at MHYAU). Unfortunately, the type collections of the species concerned could not be re-examined since type material was not available on
loan, and even attempts to examine the specimens in the herbarium MHYAU failed, i.e. the access was refused. Hence, it was necessary
to place the species concerned in the list of not examined, doubtful taxa, above all since Z.Y. Zhang and co-workers used a rather broad
concept of Cladosporium s. lat. Nevertheless, the potentially biotrophic Cladosporium species concerned are listed in this tabular key, but
they are cited in square brackets.
Acanthaceae
[Strobilanthes ......................................................................................................................................................................... C. strobilanthis]
Amaryllidaceae
1
Conidia solitary or in short unbranched or branched chains, subcylindrical-fusiform to often cylindrical, 10–45(–68) × (3–)4.5–8(–9) μm,
occasionally up to 180 μm in length, 0–4(–7)-septate, smooth or almost so; on Allium .................................................... C. victorialis
1*
Conidia solitary, wall coarsely ornamented, verrucose-digitate ............................................................................................................. 2
2
Conidiophores 60–150(–250) × 4–19 μm, conidia (40–)60–90(–120) μm long; on Allium cepa and A. istulosum ........... C. allii-cepae
2*
Conidiophores usually shorter, up to 120 μm long, and somewhat narrower, 4–13 μm; on various other Allium spp. and Narcissus
............................................................................................................................................................................................................... 3
3
Conidiogenous loci distinctly peg-like, up to 2 μm high, 1.5–2.5(–3) μm wide; on Allium spp. ..................................................... C. allii
3*
Conidiogenous loci up to 1 μm high, not peg-like, 1.5–3.5 μm wide; on Narcissus .............................................................. C. lacroixii
Anacardiaceae
Rhus ................................................................................................................................................................... C. verrucocladosporioides
Annonaceae
Annona .......................................................................................................................................................................................... C. annonae
Apiaceae
Angelica .......................................................................................................................................................................................... C. foliorum
Apocynaceae
1
Conidiophores in small to moderately large fascicles, arising from substomatal stromatic hyphal aggregations, 3–7.5 μm wide, at the
very base sometimes up to 10 μm wide; on Vinca .............................................................................................................. C. vincicola
1*
Conidiophores solitary, arising terminally or laterally from hyphae, narrower, 2.5–5 μm wide .............................................................. 2
2
Conidia 2.5–5(–6) μm wide, 1–4(–5)-septate; on Nerium .................................................................................................... C. neriicola
2*
Conidia 6.4–7.7 μm wide, 0–1-septate, rarely 2–3-septate; on Metaplexis ................................................................... [C. metaplexis]
Aquifoliaceae
Ilex .............................................................................................................................................................................................. C. cheonis
Araceae
1
Conidia (5–)6–9(–10) μm wide, smooth; biotrophic on Colocasia .................................................................................... C. colocasiae
1*
Conidia narrower, 2–6.5 μm wide, smooth to verruculose; saprobic on dead leaves of Dieffenbachia ..................... C. dieffenbachiae
Araliaceae
[Tetrapanax ............................................................................................................................................................................. C. tetrapanacis]
Arecaceae
1
Conidia 13–30(–44) × 7–12 μm, 2–4(–5)-septate, conidial wall distinctly 2–3-layered; on Corypha .................................. C. coryphae
1*
Conidia much narrower, 2–6 μm wide, wall one-layered ....................................................................................................................... 2
2
Conidiophores nodulose, with conidiogenous loci often conined to swellings; conidia 3–10(–14) μm long, 0–1-septate, smooth;
conidiogenous loci and hila small, 0.5–1(–1.5) μm diam; on Borassus ................................................................................. C. borassi
2*
Conidiophores non-nodulose, without swellings; conidia longer, up to 20 μm, 0–1(–3)-septate, usually minutely verruculose;
conidiogenous loci and hila wider, (0.5–)1–2(–2.5) μm diam; on Chamaerops ............................................................ C. chamaeropis
36
the genuS Cladosporium
Aristolochiaceae
[Aristolochia ........................................................................................................................................................................... C. aristolochiae]
Asparagaceae
1
Conidia solitary or in short unbranched chains ..................................................................................................................................... 2
1*
Conidia catenate, in branched chains ................................................................................................................................................... 4
2
Conidiophores 80–250(–300) × 5–16 μm; conidiogenous loci and hila 3–4 μm wide and 1.5–2 μm high; conidia (35–)40–70 × 18–24
μm; on Polygonatum ........................................................................................................................................................ C. polygonati
2*
Conidiophores shorter and narrower, (12–)25–130(–200) × (3–)4.5–12 μm; conidiogenous loci narrower; conidia (10–)20–60(–75) ×
(5–)7–15(–16) μm .................................................................................................................................................................................. 3
3
Conidiophores (3–)4.5–8(–10) μm; loci 1.5–2.5(–3.5) μm diam; conidia (5–)7–12 μm wide, (0–)1–3(–5)-septate, echinulate (digitate
under SEM); on Arthropodium .......................................................................................................................................... C. arthropodii
3*
Conidiophores (7–)8–12 μm, loci (2–)3(–4) μm diam; conidia (7–)9–15(–16) μm wide, (0–)2–6(–7)-septate, verrucose to coarsely
verrucose; on Ornithogalum ............................................................................................................................................. C. ornithogali
4
Conidiophores solitary ........................................................................................................................................................................... 5
4*
Conidiophores in small to very large, loose to dense fascicles ............................................................................................................. 6
5
Conidia 2.8–15.4 long; on Ophiopogon ….. .............................................................................................................. [C. ophiopogonis]
5*
Conidia 3.1–20.6 long; on Polygonatum ................................................................................................................ [C. polygonaticola]
6
Conidiophores 20–80 × 3–8 μm; conidia 4–18 μm long, 0–1-septate, on Yucca ................................................................... C. yuccae
6*
Conidiophores 20–240 × 3–5(–5.5) μm; conidia 3.5–26 μm long, 0–3-septate; on Cordyline, Dracaena .................... C. dracaenatum
Aspleniaceae
[Neottopteris ........................................................................................................................................................................ C. neottopteridis]
Asteraceae
1
Mycelium internal; conidia loosely to densely verruculose or faintly to distinctly verrucose-echinulate ................................................ 2
1*
Mycelium mainly external; conidia usually smooth, only occasionally some conidia verruculose or faintly rough-walled ..................... 6
2
With well-developed, large stromata, at irst 20–100 μm diam, later expanded, pustulate, up to 500 μm diam; conidiophores in dense
fascicles, 100–250 μm long; conidia coarsely verruculose, warts up to 1 μm diam, distant (up to 2.5 μm); on dead stems of Zinnia
........................................................................................................................................................................................... C. oblongum
2*
Stromata lacking or almost so; conidiophores solitary or in small, loose fascicles; conidia almost smooth to densely and inely
verruculose or verruculose-echinulate .................................................................................................................................................. 3
3
Conidial hila 1–1.5 μm diam; conidia (5–)10–15(–20) μm long ............................................................................................................. 4
3*
Conidial hila wider 1–3.5(4.5) μm diam; conidia distinctly longer .......................................................................................................... 5
4
Conidiophores 48–250 × 3.4–5.8 μm; conidia 8–14 × 4–8 μm; on Carpesium .................................................................... C. carpesii
4*
Conidiophores 60–100(–200) × 2.5–3 μm; conidia (3–)4–15(–20) 2–3.5(–4) μm; on Phaenocoma ......................... C. phaenocomae
5
Conidiophores 20–90(–110) × (3–)4–10(–13) μm, 0–4-septate; conidia (10–)12–40(–50) × (5–)6–13(–15) μm; conidiogenous loci and
hila (1.5–)2–3.5(–4.5) μm diam; on Agoseris ................................................................................................................... C. agoseridis
5*
Conidiophores shorter and narrower, 8–54 × (2.5–)3.5–6(–7) μm, 0–1(–2)-septate; conidia shorter and narrower, (6.5–)12–26(–31) ×
4–7(–8) μm; conidiogenous loci and hila narrower, 1–2.5 μm diam; on Tragopogon ........................................................... C. praecox
6
Conidiophores solitary to often fasciculate, arising from small stromata or creeping hyphae, 10–120 μm long; conidia 2.5–6 μm wide;
on Gynoxys ........................................................................................................................................................................ C. inopinum
6*
Conidiophores solitary or in loose to dense groups, but non-asciculate, arising from pluriseptate, external, creeping hyphae or stromatic
hyphal aggregations, much shorter, 5–50 μm long; conidia wider, (3–)5–8 μm; on Artemisia ........................................... C. obtectum
Berberidaceae
Berberis ................................................................................................................................................................................. C. ushuwaiense
Betulaceae
Alnus ............................................................................................................................................................................................... C. alneum
Bignoniaceae
1
Conidiophores arising from swollen hyphal cells and supericial hyphae, 15–500 μm long, frequently dichotomously or subdichotomously
branched; on leaves of Catalpa but also on different hosts ................................................................................................... C. varians
1*
Conidiophores much shorter, unbranched or irregularly branched ........................................................................................................ 2
2
Mycelium external; conidiophores solitary, arising from swollen hyphal cells or supericially growing, creeping hyphae, 4–113(–190)
μm long, often growing like and confusable with hyphae; conidia 3–23(–27) μm long; on Heterophragma ......... C. heterophragmatis
2*
Mycelium internal, usually substomatal; conidiophores usually fasciculate, arising from stromata, emerging through stomata, shorter,
10–58 μm long, not growing like and not confusable with hyphae; conidia shorter, 4–18 μm long; on Jacaranda ..... C. jacarandicola
www.studiesinmycology.org
37
BenSch et al.
Boraginaceae
Heliotropium ................................................................................................................................................................................ C. heliotropii
Brassicaceae
1
Conidia catenate, in longer chains, 6–33(–44) × 4–8(–9) μm, 0–4-septate; on Arabis ...................................... C. subsclerotioideum
1*
Conidia solitary or in short chains, 10–14 μm wide; on Brassica and Thelypodium .......................................................... C. brassicae
Campanulaceae
[Platycodon ............................................................................................................................................................................ C. platycodonis]
Capparaceae
1
Mycelium internal; stromata intraepidermal; conidiophores cylindrical-oblong, unbranched, rarely branched, 35–130 × 4.5–7(–9) μm;
conidia polymorphous, 4–40 × 4.5–8 μm, 0–6(–8)-septate; conidiogenous loci and hila 1–2.5 μm diam; on Boscia ........... C. bosciae
1*
Mycelium at irst internal, later also external; stromata lacking; conidiophores iliform, unbranched or branched, sometimes slightly
furcate, shorter and narrower, 8–70(–90) × 2.5–5(–6) μm; conidia shorter and narrower, 2–14(–16) × 2–4.5 μm, 0–3-septate;
conidiogenous loci and hila narrower, 0.5–1.5(–2) μm diam; on Capparis ....................................................................... C. lineolatum
Caricaceae
[Carica ........................................................................................................................................................................................ C. caricinum]
Caryophyllaceae
1
Conidia solitary or only in very short uubranched chains, (15–)25–55(–65) × (10–)12–15(–17) μm, echinulate; on Dianthus and
possibly other hosts of this family .................................................................................................................................. C. echinulatum
1*
Conidia in branched chains, much smaller and narrower, < 20(–25) × 7 μm, smooth .......................................................................... 2
2
Life cycle both sexual and asexual; ramoconidia 3–4(–4.5) μm wide; on Silene ................................................................... C. silenes
2*
Life cycle only asexual; ramoconidia 4.6–7.2 μm wide; on Lychnis ................................................................................. [C. lychnidis]
Celastraceae
Euonymus .............................................................................................................................................................................. C. subobtectum
Chenopodiaceae
Spinacia ......................................................................................................................................................................................... C. variabile
Cucurbitaceae
Citrullus, Coccinia, Cucumis, Cucurbita, Lagenaria, Luffa, Momordica, Sechium ................................................................ C. cucumerinum
Cycadaceae
Cycas
1
Conidiophores very long, usually 150–360 μm ...................................................................................................................... C. apicale
1*
Conidiophores much shorter, 20–60 μm ............................................................................................................................... C. cycadis
Dryopteridaceae
[Cyrtomium .................................................................................................................................................................................... C. cyrtomii]
Ericaceae
Rhododendron ......................................................................................................................................................................... C. rhododendri
Euphorbiaceae
1
Conidiophores 10–90 × 2.5–6 μm; conidia 4–18 μm long, smooth or almost so; on Manihot ........................................ C. nigrelloides
1*
Conidiophores 150–430 × 3–4 μm; conidia 4.5–25 (–29), smooth to loosely verruculose, irregularly verruculose-rugose or roughwalled; on Acalypha .......................................................................................................................................................... C. acalyphae
Fabaceae
1
Conidia dimorphic, with large, above all wide conidia, .23–37 × 8.5–13.5 μm, 0–6-septate, and smaller conidia, only 12.5–25 × 5–8.5
μm; on Robinia ..................................................................................................................................................................... C. robiniae
1*
Conidia not distinctly dimorphic, narrower, < 10 μm wide ..................................................................................................................... 2
2
Conidiophores at least partly solitary, arising from supericial hyphae .................................................................................................. 3
2*
Conidiophores not arising from supericial hyphae ............................................................................................................................... 6
3
Conidiophores and conidia smooth to distinctly rough-walled or rugose ………………………………………………………………….…. 4
3*
Conidiophores and conidia smooth ....................................................................................................................................................... 5
4
Conidiophores 20–300 μm long, 4–10 μm wide below and 2.5–5 μm wide in the upper half; on pods of Butea ............... C. buteicola
38
the genuS Cladosporium
4*
5
5*
6
6*
7
7*
8
8*
9
9*
10
10*
11
11*
Conidiophores 16–105 × (3.5–)4–6 μm; on Gleditsia .................................................................................................... C. gleditschiae
Conidiophores 30–200(–250) μm long; conidia 5–35(–55) × (2.5–)3.5–6.5(–7.5) μm, 0–4(–5)-septate; conidiogenous loci and hila 1–2
μm diam; on leaves of Caragana ..................................................................................................................................... C. caraganae
Conidiophores 20–100 μm long; conidia 3–11 × 2–4 μm, 0–1-septate; conidiogenous loci and hila up to 1 μm diam; on Chamaecrista
and Senna .......................................................................................................................................................... C. cassia-surathensis
Conidiophores with nodulose swellings, short, ca. 20–55 μm long; conidia smooth, 0–1-septate; on Lathyrus ................... [C. lathyri]
Conidiophores without swellings ........................................................................................................................................................... 7
Conidia in short chains, ca. 5.5–9.5 μm wide, 0–3-septate, distinctly verruculose; on Pisum ..................................... [C. qinghaiense]
Conidia in long, branched chains, or narrower, or only smooth to faintly rough-walled ......................................................................... 8
Conidiophores solitary or mostly loosely fasciculate, relatively short, 10–80 μm long; conidiogenous cells terminal, with only few
conidiogenous loci; conidia 0–3-septate ............................................................................................................................................... 9
Conidiophores solitary or in small loose groups, but non-fasciculate, longer, up to 180 μm; conidiogenous cells terminal and intercalary,
often with numerous conidiogenous loci; conidia 0–1(–3)-septate ...................................................................................................... 10
Stromata small, ca. 10 μm diam; conidia smooth; on Lupinus ...................................................................................... C. lupiniphilum
Stromata well-developed, 10–60 μm diam, later expanded, up to 150 μm wide; conidia verruculose; on dead legumes of Phaseolus
vulgaris ......................................................................................................................................................................... C. leguminicola
Conidiophores 3–7(–8) μm wide; conidiogenous loci and hila 0.5–2 μm diam; ramoconidia occasionally formed, base up to 3.5 μm
wide; conidia 3–22(–29) μm long; on Vigna ............................................................................................................................ C. vignae
Conidiophores somewhat narrower, 3–6 μm wide; conidiogenous loci and hila somewhat wider, 1–3 μm diam; ramoconidia not
occurring; conidia usually somewhat longer, 5–30 .............................................................................................................................. 11
Conidiophores 22–130 × 3–6 μm, slightly geniculate-sinuous; conidiogenous loci and hila 1–3 μm diam; conidia 5–30 × 3.5–7 μm;
on Psoralea ....................................................................................................................................................................... C. psoraleae
Conidiophores 20–180 × 3–5(–7) μm, often distinctly geniculate-sinuous; conidiogenous loci and hila 1–2.5 μm diam; conidia 3–26 ×
3–5(–7) μm; on Pisum ........................................................................................................................................................... C. pisicola
Fagaceae
Quercus ................................................................................................................................................................................... C. fumagineum
Gentianaceae
Gentiana ...................................................................................................................................................................................... C. gentianae
Hydrangeaceae
[Hydrangea .............................................................................................................................................................................. C. hydrangeae]
Iridaceae
Iris
1
Conidia (18–)30–75(–87) × (7–)10–16(–18) μm, (0–)2–6(–7)-septate, wall thickened, above all in older conidia, up to 1 μm wide
................................................................................................................................................................................................... C. iridis
1*
Conidia shorter and wider, 15–55 × (9–)11–19(–21) μm, 0–3-septate, wall thicker, up to 2 μm wide ............................. C. pseudiridis
Lamiaceae
[Teucrium .......................................................................................................................................................................................... C. teucrii]
Lauraceae
Ocotea ................................................................................................................................................................................... C. oreodaphnes
Liliaceae
[Liliaceae s. lat. see also Amaryllidaceae (Allium) and Asparagaceae (Arthropodium, Ornithogalum, Polygonatum)] ............. C. ornithogali
Magnoliaceae
1
Stromata developed, 15–40 μm diam; conidiophores solitary or in small loose groups; ramoconidia lacking; causing leaf-spots on
Liriodendron ..................................................................................................................................................................... C. liriodendri
1*
Stromata lacking or almost so; conidiophores solitary or in small to moderately large fascicles; ramoconidia present; on dead leaves
of Magnolia ......................................................................................................................................................................... C. delectum
Malvaceae
[Corchorus ..................................................................................................................................................................................... C. corchori]
Melanthiaceae
Trillium .................................................................................................................................................................................................. C. trillii
www.studiesinmycology.org
39
BenSch et al.
Myrtaceae
1
Conidial chains labellate, spread in a fan-like manner, conidia 2.5–3(–3.5) μm wide, aseptate, smooth; on Melaleuca
....................................................................................................................................................................................... C. labelliforme
1*
Conidial chains not labellate, branching in all directions, occasionally dichotomously branched, conidia somewhat wider, (2–)2.5–4
μm wide, 0–2-septate, inely verruculose, irregularly rough-walled or slightly to distinctly irregularly verruculose-rugose ................... 2
2
Conidiophores asperulate or irregularly rough-walled; conidia 4–30(–37) × (2.5–)3.5–4.5(–5) μm, slightly to distinctly verruculoserugose; on Eucalyptus .................................................................................................................................................. C. exasperatum
2*
Conidiophores smooth or almost so; conidia 5–20(–25) × 3–4(–4.5) μm, smooth to inely verruculose or somewhat irregularly roughwalled; on Corymbia and Eucalyptus .......................................................................................................................... C. myrtacearum
Nartheciaceae
Narthecium .................................................................................................................................................................................... C. ossifragi
Nyctaginaceae
Bougainvillea .......................................................................................................................................................................... C. arthrinioides
Oleaceae
1
Conidiophores dimorphic; conidiogenous cells with numerous subdenticulate, often crowded conidiogenous loci, apex appearing
somewhat rugose (periconiella-like); conidia 2.5–18 × 2–5(–6.5) μm; on Syringa .......................................................... C. syringicola
1*
Conidiophores non-dimorphic; conidiogenous cells with a single to several conidiogenous loci, not crowded, apex not periconiellalike ......................................................................................................................................................................................................... 2
2
Conidiophores up to 265 μm long, (2.5–)4–9 μm wide; on Fraxinus ................................................................................ C. fraxinicola
2*
Conidiophores much shorter and narrower, ca. 20–70 × 4.4–5.9 μm; on Forsythia ........................................................ [C. forsythiae]
Onagraceae
[Circaea ......................................................................................................................................................................................... C. circaeae]
Orchidaceae
1
Conidiophores characteristically branched, right-angled; on Epidendrum ................................................................... C. rectangulare
1*
Conidiophores unbranched, rarely sparingly branched, but not right-angled ........................................................................................ 2
2
Conidiophores solitary, rarely in small groups, not emerging through stomata; on Stanhopea ...................................... C. stanhopeae
2*
Conidiophores loosely to densely fasciculate, rarely solitary, usually emerging through stomata ........................................................ 3
3
Conidiophores 50–175 μm long, pluriseptate, often entero-blastically proliferating, often nodulose with small intercalary swellings;
conidiogenous cells terminal and intercalary, with numerous, crowded conidiogenous loci; conidia subglobose, broadly ovoid, ellipsoid
to somewhat irregular, smooth to minutely verruculose; loci and hila 0.5–1.5 μm wide; on Cypripedium .................. C. orchidiphilum
3*
Conidiophores shorter, 20–75 μm long, 0–3-septate, not enteroblastically proliferating, without swellings; conidiogenous cells terminal,
only few conidiogenous loci, not crowded; conidia obovoid, ellipsoid to subcylindrical, usually verruculose; loci and hila wider, (1–)1.5–
2.5 μm diam; on Oncidium ........................................................................................................................................... C. orchidearum
Passiloraceae
Passilora ...................................................................................................................................................................................... C. maracuja
Phrymaceae
Mimulus ..................................................................................................................................................................................... C. mimulicola
Phytolaccaceae
Rivina ................................................................................................................................................................................................ C. rivinae
Pinaceae
Pinus
1
Conidia 0–3-septate, almost smooth to usually verruculose, sometimes verrucose in vivo; in vitro conidiophores macronematous,
(2.5–)3.5–5.5 μm wide, terminal conidial chains long, up to nine conidia .............................................................. C. pini-ponderosae
1*
Conidia 0–1-septate, almost smooth to minutely verruculose; in vitro conidiophores both macro- and micronematous, (1.5–)2.5–4 μm
wide, up to three or four conidia in the terminal unbranched part of the chain ............................................................... C. chubutense
Piperaceae
Piper ........................................................................................................................................................................................... C. pipericola
Plantaginaceae
Melosperma ........................................................................................................................................................................... C. melospermae
40
the genuS Cladosporium
Poaceae
1
Conidiophores solitary, non-fasciculate, arising from internal and external creeping hyphae, 5–90 × 3–5(–6) μm; conidia catenate,
in unbranched or branched chains, narrow, 3–41(–60) × 3.5–5.5(–7) μm, 0–4(–7)-septate, septa often distinctly darkened, smooth;
conidiogenous loci and hila narrow, 0.5–1.5(–2) μm diam; on Cenchrus and Chaetochloa .......................................... C. spongiosum
1*
Conidiophores fasciculate, longer and wider; conidia solitary or only in short chains, rather large, about 23–40(–60) × 6–15 μm,
verruculose ........................................................................................................................................................................................... 2
2
Conidiophores in usually fairly large, dense fascicles, some-times in palisade-like layers, arising from stromata, 40–120 × 5–9 μm;
conidia solitary, 0–1-septate; conidiogenous loci and hila 3–5 μm diam; on Alopecurus ................................................... C. alopecuri
2*
Conidiophores solitary or in small, loose fascicles, up to 300 μm long, 6–9 μm wide; conidia solitary or in short chains, (0–)1–3(–5)-septate;
conidiogenous loci and hila 2–2.5 μm diam; on Phleum ............................................................................................................ C. phlei
Polypodiaceae
[Neocheiropteris ............................................................................................................................................................ C. neocheiropteridis]
Primulaceae
1
Stromata lacking; conidiophores solitary or loosely aggregated, 100–400 μm long; on dead leaves of Primula ............... C. auriculae
1
Stromata 40–150 μm diam; conidiophores in large, dense fascicles, sometimes even subsynnematous; on Soldanella
........................................................................................................................................................................................ C. soldanellae
Proteaceae
1
Sexual part of the life cycle formed; asci, ascospores; on Grevillea ................................................................................. C. grevilleae
1*
Asexual part of the life cycle formed; conidiophores and conidia; on Alloxylon ....................................................... C. angustisporum
Ranunculaceae
Ranunculus
1
Conidia solitary, very large, ca. 30–60 × 7.5–9 μm ................................................................................................................... C. ferox
1*
Conidia catenate, much smaller, (2.5–)5–23 × (2.5–)3.5–7.5 μm ............................................................................ C. grech-delicatae
Rosaceae
1
Conidia (2–)3–45 × 2–8 μm, 0–6-septate; conidiogenous loci and hila 1–2.5(–3) μm diam; on Photinia ....................... C. diaphanum
1*
Conidia smaller, up to 22 μm long, 0–3(–4)-septate; conidiogenous loci and hila 0.5–2 μm diam; on Eriobotrya ................................ 2
2
Conidiophores dimorphic, 50–300 μm long, sometimes branched, not geniculate; conidia 3.5–14(–20) × 2.5–6 μm, 0–1(–2)-septate,
very pale, smooth or almost so ............................................................................................................................... C. fusicladiiformis
2*
Conidiophores not dimorphic, 20–120 μm long, unbranched, usually distinctly geniculate-sinuous; conidia up to 22 μm long,
0–3(–4)-septate, distinctly verruculose ........................................................................................................................... C. eriobotryae
Rubiaceae
Galium ................................................................................................................................................................................................... C. galii
Rutaceae
1
Conidia solitary or occasionally in short chains, rather broad, (15–)18–30 × 9–13 μm; on Haplophyllum ...................... C. haplophylli
1*
Conidia catenate, narrower, 3–7 μm wide ............................................................................................................................................. 2
2
Conidiophores 10–40 μm long, 0–1(–2)-septate; conidiogenous cells terminal or conidiophores often reduced to conidiogenous cells;
conidia 0–1-septate; conidiogenous loci and hila 1.5–2.5 μm diam; on Ruta ........................................................................... C. rutae
2*
Conidiophores longer, 15–70 μm long, 0–5-septate; conidiogenous cells terminal or intercalary; conidia 0–2(–3)-septate; conidiogenous
loci and hila narrower, 0.5–1.5(–2) μm diam; on Citrus .................................................................................................. C. corrugatum
Salicaceae
1
Conidiophores mostly strongly and frequently geniculate-sinuous, 15–100 × 2–5(–7) μm; conidiogenous cells 0.5–1.5 μm diam; on
Salix ............................................................................................................................................................................... C. minusculum
1*
Conidiophores longer and wider, 50–175 × (2.5–)3–8(–11) μm, only somewhat geniculate-sinuous or subnodulose; conidiogenous loci
and hila 1–2 μm diam ............................................................................................................................................................................ 2
2
Conidiophores (4–)5–8(–11) μm wide; conidia 4–14 × 3–5(–5.5) μm, 0–1-septate; on Populus ..................................... C. populicola
2*
Conidiophores (2.5–)3–6(–7) μm wide; conidia 3–20(–25) × 2.5–6(–7) μm, 0–3-septate; on Oncoba ............................... C. oncobae
Sapindaceae
Aesculus ........................................................................................................................................................................... C. asperistipitatum
Sapotaceae
Chrysophyllum ......................................................................................................................................................................... C. chrysophylli
www.studiesinmycology.org
41
BenSch et al.
Scrophulariaceae
[Digitalis .................................................................................................................................................................................... C. digitalicola]
Smilacaceae
Smilax ........................................................................................................................................................................................ C. smilacicola
Styracaceae
Styrax ................................................................................................................................................................................... C. inconspicuum
Thymelaeaceae
[Edgeworthia ......................................................................................................................................................................... C. edgeworthiae]
Typhaceae
Typha ....................................................................................................................................................................................... C. heleophilum
Ulmaceae
Ulmus
1
Conidiophores 58–325 × 3.5–9(–11) μm; ramoconidia up to 60 μm long, 0–3(–5)-septate; conidia polymorphous, 2–32 × 2–7 μm,
0–2-septate; conidiogenous loci and hila 0.5–3 μm diam; on Ulmus laevis and additional hosts ......................................... C. varians
1*
Conidiophores much shorter and narrower, 10–80(–100) × (2–)2.5–4(–5) μm; ramoconidia absent; conidia shorter and narrower,
4–17(–19) × 2–5 μm, 0–1(–3)-septate; conidiogenous loci and hila narrower, 0.5–1.5 μm; on Ulmus minor .............. C. hypophyllum
Xanthorrhoeaceae
[Dianella ................................................................................................................................................................................... C. dianellicola]
Xyridaceae
Xyris ................................................................................................................................................................................................ C. xyridis
Zingiberaceae
[Alpinia ............................................................................................................................................................................................ C. alpiniae]
Key to fungicolous and lichenicolous Cladosporium species in vivo based on morphology and ecology
Beside fungicolous and lichenicolous Cladosporium species, this key also contains some common, widespread saprobic species which
occasionally occur on other fungi and lichens.
1
With numerous globose to subglobose terminal conidia, almost smooth to verruculose; conidiophores often branched; saprobic species
............................................................................................................................................................................. C. sphaerospermum
1*
Without globose terminal conidia .......................................................................................................................................................... 2
2
Conidia verruculose, rarely a few intermixed conidia smooth or almost so ........................................................................................... 3
2*
Conidia smooth or almost so, rarely a few intermixed conidia faintly rough-walled .............................................................................. 8
3
Conidiophores distinctly nodulose, swellings round about the stalk; conidiogenous loci conined to swellings; saprobic species,
occasionally occurring on other fungi and lichens as secondary invader .............................................................................................. 4
3*
Conidiophores non-nodulose, straight, subcylindrical to geniculate-sinuous ........................................................................................ 5
4
Swellings of the conidiophores up to 8 μm diam; conidia in branched chains, secondary ramoconidia usually (3–)5–7(–8) μm wide
........................................................................................................................................................................................... C. herbarum
4*
Swellings of the conidiophores wider, up to 12 μm diam; conidia in vivo dimorphic, smaller conidia in branched chains, mixed with at
least some very large conidia, formed singly or in short simple chains, (6–)7–12(–15) μm wide ............................... C. macrocarpum
5(3) Conidiophores often irregularly to dichotomously branched; on Exobasidium .................................. C. exobasidii var. verruculosum
5*
Conidiophores unbranched, rarely with a single branching ................................................................................................................... 6
6
Occasionally with ramoconidia; isolated from Caloplaca regalis, Antarctica .................................................................. C. antarcticum
6*
Ramoconidia lacking; on aecia of rust fungi or Epichloë typhina .......................................................................................................... 7
7
On Epichloë typhina .......................................................................................................................................................... C. epichloës
7*
On aecia of rust fungi ....................................................................................................................................................... C. aecidiicola
8(2) Conidiophores nodulose, with conspicuous swellings round about the stalk, swellings with conidiogenous loci .................................. 9
8*
Conidiophores non-nodulose .............................................................................................................................................................. 11
9
Conidiophores often simply branched; on Exobasidium ......................................................................... C. exobasidii var. exobasidii
9*
Conidiophores unbranched ................................................................................................................................................................. 10
9
Conidiophores very long, about up to 500 μm or even longer, swellings regular, round about the stalk, and remote; saprobic species
......................................................................................................................................................................................... C. oxysporum
42
the genuS Cladosporium
9*
Conidiophores much shorter, up to 80 μm, swellings lacking or present, if present not remote and rather irregular; on Taphrina
tosquinetii ................................................................................................................................................. ‘C. herbarum f. parasiticum’
11(8) Conidiophores very long, up to 550(–800) μm, iliform-etiform, unbranched; conidiogenous cells terminal, often somewhat swollen;
conidia 0–1-septate; saprobic species ......................................................................................................................... C. tenuissimum
11* Conidiophores usually much shorter or branched; speciic, fungicolous or lichenicolous species ...................................................... 12
12 Conidiophores frequently branched, branched part terminal, often dense and periconiella-like; conidiogenous loci often numerous and
crowded at the tips of the branchlets ................................................................................................................................................... 13
12* Conidiophores simple or irregularly branched, branching not conined to the apex, not periconiella-like; conidiogenous loci usually not
crowded ............................................................................................................................................................................................... 14
13 With well-developed stromata, 45–130 × 30–90 μm; conidia up to 29 μm long, 2–3(–4)-septate; on rust fungi (Cronartium,
Endocronartium and Pucciniastrum) .................................................................................................................................... C. gallicola
13* Stromata lacking; conidia 3.5–13 μm long, 0–1-septate; lichenicolous, on Parmelia, Pertusaria, Phaeophyscia, Physcia and Xanthoria
..................................................................................................................................................................................... C. licheniphilum
14(12) Conidiophores thick-walled throughout or at least towards the base, thickened walls conspicuously two-layered (light microscopy);
conidia always thick-walled, wall partly two-layered as well ................................................................................................................ 15
14* Walls of the conidiophores and conidia always one-layered (light microscopy), thin to slightly thickened .......................................... 16
15 Walls of the conidiophores two-layered throughout, septa strongly darkened; hila conspicuously protuberant, up to 1.5 μm high;
ramoconidia lacking; on Monilinia laxa and Boletales ................................................................................................. C. episclerotiale
15* Conidiophores only two-layered towards the base, septa not conspicuously darkened; hila less protuberant; ramoconidia present; on
Gerwasia (rust fungus) ...................................................................................................................................................... C. gerwasiae
16(14) Colonies on fruit bodies of macromycetes (gilled fungi, gastero-mycetes, poroid fungi) ................................................................... 17
16* Colonies on pathogenic fungi (rust fungi, powdery mildews, downy mildews or Taphrina spp.) ..........................................................18
17 Ramoconidia frequently formed, 33–53 × 4.5–7 μm, 0–6-septate ..................................................................................... C. epimyces
17* Ramoconidia rarely formed, 21–32 × 4.5–5.5 μm, 0–4(–5)-septate ........................................................................... C. lycoperdinum
18(16) Conidiophores erect to frequently decumbent, repent, growth characteristically effuse; ramoconidia present; conidia (0–)1–5(–6)-septate;
conidiogenous loci and hila (0.5–)1–3 μm diam; on Taphrina ...................................................................................... C. phyllophilum
18* Conidiophores usually erect, neither repent nor effuse; ramoconidia lacking; conidiogenous loci and hila smaller, 0.5–2 μm diam
............................................................................................................................................................................................................. 19
19 Conidia 4–20 μm long; mycelium in asci of Taphrina coerulescens ................................................................................... C. taphrinae
19* Conidia up to 40 μm long; mycelium in sori of rust fungi, on powdery mildew or downy mildew .................................... C. uredinicola
Tabular key to fungicolous and lichenicolous Cladosporium species based on hosts
Ascomycota (lichenised) ................................................................................................................................................................................ 43
Ascomycota (non-lichenised) ........................................................................................................................................................................ 43
Basidiomycota ............................................................................................................................................................................................... 44
Peronosporomycetes (Downy mildews) ........................................................................................................................................................ 44
Lichenised Ascomycota
1
1*
Conidiophores subcylindrical or only slightly geniculate-sinuous, 5–8 μm wide; conidia smooth, 3.5–13 × 3–7 μm; on Parmelia,
Pertusaria, Phaeophyscia, Physcia and Xanthoria ..................................................................................................... C. licheniphilum
Conidiophores usually distinctly geniculate-sinuous, 3–4.5 μm wide; isolated from the thallus of Caloplaca regalis .... C. antarcticum
Non-lichenised Ascomycota
Capnodiales
Teratosphaeria .............................................................................................................................................................. C. chalastosporoides
Erysiphales (powdery mildews)
1
Conidiophores 6–105(–120) μm long; conidia 3–17(–24) × (2–)3–4.5 μm, 0–1(–2)-septate; on Phyllactinia guttata
.................................................................................................................................................................................. C. phyllactiniicola
1*
Conidiophores (5–)20–230(–300) μm long; conidia 3–39 × 3–6.5(–8) μm, 0–3(–5)-septate; on Erysiphe and Phyllactinia spp.
......................................................................................................................................................................................... C. uredinicola
Helotiales (Monilinia)
Monilinia laxa (Sclerotinia cinerea) ........................................................................................................................................ C. episclerotiale
Hypocreales (Epichloë)
Epichloë typhina .......................................................................................................................................................................... C. epichloës
www.studiesinmycology.org
43
BenSch et al.
Taphrinales (Taphrina)
1
Conidiophores erect to decumbent, repent; ramoconidia present ................................................................................ C. phyllophilum
1*
Conidiophores erect, usually not decumbent; ramoconidia lacking ....................................................................................................... 2
2
Conidiophores usually arising from hyphae, 23–150(–200) × 3–5(–7) μm, without intercalary swellings; conidiogenous loci usually
numerous, crowded, denticle-like; conined to asci of Taphrina coerulescens ................................................................... C. taphrinae
2*
Conidiophores usually arising from stromata, 52–82 × 3.5–5.5 μm, occasionally with intercalary swellings, up to 6 μm diam, swellings
with conidiogenous loci, but loci not conined to these swellings; on Taphrina tosquinetii ....................... ‘C. herbarum f. parasiticum’
Basidiomycota
Exobasidiales (Exobasidium)
1
Conidia smooth ....................................................................................................................................... C. exobasidii var. exobasidii
1*
Conidia verruculose ........................................................................................................................... C. exobasidii var. verruculosum
Pucciniales (Uredinales)
1
Conidia verruculose, rarely a few conidia only faintly rough-walled; on aecia .................................................................. C. aecidiicola
1*
Conidia smooth ..................................................................................................................................................................................... 2
2
Conidiophores frequently branched in the upper part, simply branched to several times, dense, periconiella-like; on aecia of Cronartium,
Endocronartium and Pucciniastrum .................................................................................................................................... C. galliicola
2*
Conidiophores usually unbranched, rarely a few conidiophores simply branched ................................................................................ 3
3
Conidiophores solitary, arising from hyphae, (5–)21–230(–300) × 2–5 μm, walls of the conidiophores only slightly thickened, 0.5–0.75
μm; ramoconidia lacking; on various rust fungi ............................................................................................................... C. uredinicola
3*
Conidiophores in loose to dense fascicles, arising from stromata, larger, 24–366(–630) × 3–8 μm, tips often elongated and
narrowed, walls of the conidiophores mostly strongly thickened towards the base, (0.5–)1–1.5 μm; ramoconidia present; on
Gerwasia ........................................................................................................................................................................... C. gerwasiae
On fruit bodies of macromycetes (Agaricales, Boletales, Polyporales, Russulales)
1 Walls of the conidiophores conspicuously two-layered; conidia subglobose, ellipsoid to broadly ellipsoid, often constricted at the septa
which are conspicuously darkened, walls of the conidia often two-layered; ramoconidia lacking; on Boletales ............. C. episclerotiale
1* Walls of the conidiophores not two-layered; conidia polymorphous; ramoconidia present (rarely to frequently formed) .......................... 2
2 Ramoconidia frequently formed, 33–53 × 4.5–8 μm, 0–6-septate; on Agaricales and Russulales ........................................ C. epimyces
2* Ramoconidia rarely formed, shorter, 11–32 × (4.5–)5–6 μm, 0–4-septate; on Agaricales, Boletales, Polyporales and Russulales
........................................................................................................................................................................................ C. lycoperdinum
Cladosporium on aphids
On aphids and supericially on leaves attacked by aphids .............................................................................................................. C. aphidis
RESULTS
Phylogenetic analysis
Approximately 1 700 bases, spanning the ITS and LSU regions,
were obtained for isolates listed in Table 1. These two regions
were analysed separately; ITS, together with EF-1α and ACT, to
determine species level relationships and LSU for the generic
placement. Approximately 350–600 and 230 bases were determined
for EF-1α and ACT, respectively, and these were concatenated with
the corresponding ITS sequences for a combined analysis of the
Cladosporium species with ex-type or representative strains.
The manually adjusted LSU alignment contained 98 taxa
(including the outgroup sequence) and, of the 837 characters used
in the phylogenetic analysis, 408 were parsimony-informative, 50
were variable and parsimony-uninformative and 379 were constant.
Neighbour-joining analyses using the three substitution models on
the sequence data yielded trees with similar topology and bootstrap
values; 528 equally most parsimonious trees were obtained from
the heuristic search, the irst of which is shown in Fig. 2 (TL = 2
302, CI = 0.375, RI = 0.827, RC = 0.310). The phylogenetic tree
44
derived from this region (Fig. 2) shows that Cladosporium s.str. is
restricted whereas Cladosporium-like genera are distributed across
many different orders, including but not restricted to Corticiales,
Pleosporales, Helotiales, Chaetothyriales etc. These genera are
discussed in more detail elsewhere in this manuscript.
The manually adjusted combined ITS, EF-1α and ACT
alignment contained 70 taxa (including the outgroup sequence)
and, of the 1 112 characters used in the phylogenetic analysis, 385
were parsimony-informative, 212 were variable and parsimonyuninformative and 515 were constant. Neighbour-joining analyses
using the three substitution models on the sequence data yielded
trees with similar topology and bootstrap values. Twelve equally
most parsimonious trees were obtained from the heuristic search,
the irst of which is shown in Fig. 5 (TL = 3 415, CI = 0.372, RI
= 0.636, RC = 0.237). The phylogenetic tree of these combined
regions (Fig. 5) shows that the ex-type and representative strains
known from sequence can be placed in one of three species
complexes, the exceptions being C. salinae and C. aphidis, and
with C. langeronii and C. psychrotolerans not occurring on the
same branch as the rest of the species in the C. sphaerospermumcomplex but as a more basal sister clade.
the genuS Cladosporium
Alphabetical descriptive list of true Cladosporium
species
The present work provides a comprehensive treatment of all
biotrophic, foliicolous, fungicolous and saprobic Cladosporium
species known to date. Cladosporium species formerly referred
to the genus Heterosporium described in detail by David (1997)
are also included. Species of uncertain taxonomic position and
excluded species are separately listed. Names are arranged in
alphabetical order. All species are comprehensively described and
illustrated. Drawings (1: 100) are, if not otherwise stated, based
on type material or other authentic collections. The scale bars
represent 10 μm. The treatments of the particular species contain
the scientiic names with bibliographic references, synonyms, type
collections, literature references, published illustrations, exsiccatae,
comprehensive descriptions, host range and geographic
distribution, additional collections examined and notes. Author
abbreviations conform to those of Brummit & Powell (1992), while
journal abbreviations follow Lawrence et al. (1968) and Bridson &
Smith (1991). Herbarium names have been abbreviated according
to Holmgren et al. (1990). Abbreviations of exsiccatae conform to
those of Triebel & Scholz (2001−2011). The host and distribution
data indicate the known distributions and hosts or substrates
obtained from checklists, herbarium specimens, databases and
other sources compiled for different countries. Host names have
been checked as far as possible and conform to “Tropicos”. Under
“additional collections examined” all specimens seen are cited,
arranged in alphabetical order. The recently published checklist
(Dugan et al. 2004) served as source for the compilation of the taxa
treated in this monograph.
1. Cladosporium acalyphae Bensch, H.D. Shin, Crous & U.
Braun, Stud. Mycol. 67: 17. 2010. Figs 6–8.
Holotype: South Korea, Hoengseong, N37º32’09’’ E128º07’07’’,
isol. from Acalypha australis (Euphorbiaceae), 11. Oct. 2004, coll.
H.-D. Shin, isol. P.W. Crous (CBS H-20422). Ex-type culture: CBS
125982 = CPC 11625.
Ill.: Bensch et al. (2010: 18−20, igs 2−4).
In vitro: Mycelium internal and supericial; hyphae unbranched or
loosely branched, iliform to cylindrical-oblong, 1−4 μm wide, later
up to 7 μm wide, especially towards the base of conidiophores,
pluriseptate, not constricted or in wider hyphae slightly constricted
at septa, sometimes septa in short succession, smooth or
minutely verruculose, walls unthickened or slightly thick-walled.
Conidiophores solitary, macronematous, arising terminally and
laterally from ascending or plagiotropous hyphae, erect, straight
to somewhat lexuous, very long, narrowly cylindrical-oblong,
150−430 × (2.5−)3−4(−5) μm, unbranched or once branched,
branches often rather long, appearing like a conidiophore on its
own, non-nodulose, sometimes once geniculate, often slightly
attenuated towards the apex, pluriseptate, cells rather long, not
constricted at septa, medium olivaceous-brown, smooth, walls
slightly thickwalled. Conidiogenous cells integrated, terminal and
sometimes intercalary, narrowly cylindrical-oblong, non-nodulose,
occasionally once geniculate-sinuous, 23−80 μm long, with 1−4
loci at the apex, occasionally few additional loci at a lower level,
but mostly above the septum, loci conspicuous, subdenticulate to
www.studiesinmycology.org
Fig. 6. Cladosporium acalyphae (CBS 125982). Macronematous conidiophores,
mycelium, ramoconidia and conidial chains in vitro. Scale bar = 10 µm. K. Bensch
del.
denticulate, 1.5−2 μm diam, somewhat thickened and darkenedrefractive. Ramoconidia cylindrical-oblong, 21−44(−65) ×
3.5−4(−4.5) μm, 0(−1)-septate, base truncate, 2−2.5 μm wide,
somewhat refractive. Conidia catenate, in branched chains,
branching in all directions or dichotomously, 1−4 conidia in the
terminal unbranched part of the chain, small terminal conidia
globose, subglobose to obovoid, broad, 4.5−9 × 4.5−6 μm (av.
± SD: 6.9 ± 1.3 × 5.0 ± 0.5), aseptate, apex broadly rounded,
base attenuated, hilum often on a short stalk-like prolongation,
intercalary conidia ovoid, ellipsoid to subcylindrical, often with
rostrate ends, (6−)8−17(−21) × 3.5−5(−6) μm (av. ± SD: 11.3 ± 3.3
× 4.4 ± 0.7), aseptate, attenuated towards apex and base, small
terminal and intercalary conidia smooth to loosely verruculose,
irregularly verruculose-rugose or rough-walled (LM), surface with
irregularly reticulate structure or embossed stripes under SEM
probably caused by diminishing turgor and shriveling of young
conidia, thin-walled, with 1−3(−4) hila at the apex, secondary
ramoconidia ellipsoid to subcylindrical or cylindrical, 12−25(−29)
× 3−5 μm (av. ± SD: 18.4 ± 4.5 × 3.9 ± 0.6), aseptate, rarely
1-septate, pale to medium olivaceousbrown, smooth or inely
verruculose, walls slightly thickened, hila conspicuous, often
situated on small peg-like prolongations, subdenticulate to
denticulate, 0.8−2 μm diam, somewhat thickened and darkenedrefractive; microcyclic conidiogenesis occurring.
Culture characteristics: Colonies on PDA attaining 60−72 mm
diam after 14 d, olivaceous-grey to grey-olivaceous, reverse
leaden-grey to iron-grey, powdery to loccose, margins colourless
to grey-olivaceous, narrow, feathery, regular, aerial mycelium
loose, diffuse to loccose or luffy, mainly in colony centre,
45
BenSch et al.
Fig. 7. Cladosporium acalyphae (CBS 125982). A–G. Macronematous conidiophores and conidial chains. Scale bars = 10 µm.
olivaceous-grey, growth effuse, without prominent exudates,
sporulation profuse. Colonies on MEA reaching 56−64 mm diam
after 14 d, grey-olivaceous to pale olivaceous-grey and iron-grey
towards margins, somewhat zonate, reverse iron-grey, powdery
to loccose, margins colourless to white, feathery, regular, aerial
mycelium diffuse to loccose, pale olivaceous-grey, mainly in
colony centre, growth effuse, radially furrowed in the centre,
without prominent exudates, sporulation profuse. Colonies on OA
attaining 59−67 mm diam after 14 d, dark smoke-grey to brownish,
iron-grey towards margins, reverse leaden-grey to iron-grey,
powdery to loccose, margins grey-olivaceous, glabrous, regular,
aerial mycelium diffuse to loccose, white to pale olivaceousgrey, growth effuse with numerous not very prominent exudates,
sporulation profuse.
Substrate and distribution: On Acalypha australis; South Korea.
Notes: Cladosporium acalyphae is genetically close to C.
delicatulum and C. inversicolor (Bensch et al. 2010, ig. 1, part a),
but constitutes a distinct lineage in TEF as well as ACT (distance
analyses in TreeBASE), but it is morphologically quite distinct from
the latter two species and all other taxa of Cladosporium.
Fig. 8. Cladosporium acalyphae (CBS 125982). A. Secondary ramoconidia and
conidia on a conidiophore. Note the smooth surface of the conidiophores. B–C.
Secondary ramoconidia on smooth conidiophores and patterns of scars. D. Details
of scars on a secondary ramoconidium. E. Secondary ramoconidia and scars.
F. Conidia as seen with cryoSEM showing a reticulate surface ornamentation.
G. SEM micrograph of “meristematic development” on the agar surface and two
conidiophores formed. Scale bars = 2 (D–E), 5 (B–C, F), 10 (A, G) µm.
46
the genuS Cladosporium
Thümen, Mycoth. Univ. 373 (e.g., B 700006146, BPI 426074, HAL,
HBG, M-0057483) and Germany, Bayern, Bayreuth, May 1874, ex
herb. Thümen, in herb. Körnick (B 700006147).
Lit.: Saccardo (1886: 368, 1913a: 1371), Lindau (1907: 806–807,
1910: 796), Ferraris (1912: 350), Gonzáles-Fragoso (1927: 211),
Ellis (1976: 330), Ellis & Ellis (1985: 571, 1988), Sagdullaeva et
al. (1990: 43), Braun & Rogerson (1995: 142), Guo (2001: 464),
Zhang et al. (2003: 36–37), Heuchert et al. (2005: 14–17).
Ill.: Ellis (1976: 330, ig. 248), Zhang et al. (2003: 36, ig. 11),
Heuchert et al. (2005: 15, ig. 1).
Exs.: Clements & Clements, Crypt. Format. Colorad. 270;
Litschauer & Kreissler, Krypt. Exs. 2938; Săvulescu, Herb. Mycol.
Roman. 1342; Spegazzini, Dec. Mycol. Ital. 57; Sydow, Mycoth.
March. 799, 3285; Thümen, Mycoth. Univ. 373. Vill, Fungi Bav. 898.
Fig. 9. Cladosporium aecidiicola (B 700006144). Conidiophores arranged in
fascicles and conidia in vivo. Scale bar = 10 µm. B. Heuchert del.
2. Cladosporium aecidiicola Thüm., Mycoth. Univ., Cent.
IV, No. 373. 1876. Figs 9–11.
Lectotype (designated here): Germany, Bayern, Bayreuth, on
aecia on living leaves of Euphorbia cyparissias (Euphorbiaceae),
1874, Thümen, Mycoth. Univ. 373 (B 700006144). Isolectotypes:
In vivo: Colonies on aecia (usually completely overgrown by the
fungus) and surrounding leaf tissue (colonies not vein-limited),
occasionally also on spermogonia, olivaceous-brown to dark
brown, occasionally almost blackish brown, caespitose, velvety,
effuse, conluent. Mycelium supericial to immersed, hyphae
branched, 2.5–6 μm wide, septate, cells 10–15 μm long, often
constricted at septa and with swellings, sometimes forming strands,
subhyaline to pale olivaceous or olivaceous-brown, smooth, walls
somewhat thickened. Stromata lacking to diffuse, composed of
subglobose cells, 3–7 μm diam, pale olivaceous-brown to medium
brown, smooth, walls somewhat thickened. Conidiophores solitary,
caespitose or in loose to dense fascicles, arising from diffuse
stromatic layers, if arising from the attacked surrounding leaf
tissue erumpent through the cuticle, erect, straight to geniculatesinuous, occasionally subnodulose, simple, occasionally branched,
10–140(–185) × 2.5–7(–8) μm, often swollen at the very base, 6–12
μm wide, 0–5(–7)-septate, sometimes constricted at the septa,
pale olivaceous-brown to medium brown, paler towards the apex,
smooth, walls thickened, up 1.5 μm wide, at the base sometimes
up to 2 μm wide. Conidiogenous cells integrated, terminal and
Fig. 10. Cladosporium aecidiicola (B 700006207). Conidiophores arranged in loose or dense fascicles and conidia. Scale bar = 10 µm. B. Heuchert del.
www.studiesinmycology.org
47
BenSch et al.
Fig. 11. Cladosporium aecidiicola (B 700006144). A. Loosely fasciculate conidiophores. B. Conidia. C. Conidium as seen with SEM showing the verruculose surface
ornamentation. D. Details of scars on conidiophores and conidia. Scale bars = 5 (C–D), 10 (A–B) µm.
intercalary, 10–67 μm long, polyblastic, sympodially proliferating,
1–4 conidiogenous loci per cell, loci sometimes located at small
shoulders formed by sympodial proliferation, protuberant,
thickened and darkened, 1–2.5 μm diam. Conidia catenate,
mostly in branched chains, straight, ovoid, ellipsoid, subglobose,
subcylindrical to fusiform, 4–25(–29) × 3–8(–10) μm, 0–3-septate,
not or occasionally somewhat constricted at septa, pale olivaceousbrown, smooth or almost so to verruculose, walls unthickened or
only slightly thickened, apex rounded to subtruncate, with up to two
hila, base truncate to convex, hila protuberant, 1–2(–2.5) μm diam,
thickened and darkened; microcyclic conidiogenesis occurring.
Substrates and distribution: On aecia of rust fungi; Asia,
Caucasus, Europe, North America – on Aecidium carneum on
Astragalus sp. (Sweden), A. cyparissiae on Euphorbia cyparissias
(Germany), A. euphorbiae (Italy, Romania, Ukraine), A. falcariae
on Falcaria sp. (Germany), A. ligustri on Ligustrum vulgare
(Germany), A. lonicerinum on Lonicera xylosteum (Austria),
A. trollii on Trollius sp. (Sweden), Aecidium sp. on Elaeagnus
oxycarpa (Kazakhstan), Aecidium sp. on Euodia sp. (China),
Coleosporium campanulacearum on Campanula sp. (Austria,
Georgia), C. campanulacearum on Campanula rapunculoides
(Austria), on C. tussilaginis on Petasites sp. (Italy), Melampsora
helioscopiae (Romania), Puccinia agropyrina on Thalictrum minus
(= T. collinum) (Kazakhstan), P. circaeae (Romania), P. coronata
(Romania), P. graminis on Berberis sp. (Armenia), P. grindeliae
on Chrysothamnus viscidilorus var. lanceolatus (USA, UT), P.
passerinii (Ukraine), P. phragmitis (China, UK), P. phragmitis on
Rumex crispus (Kazakhstan), P. smilacearum-digraphidis on
Polygonatum verticillatum (Germany), P. tragopogi (Armenia),
P. violae (Romania), Puccinia sp. on Hordeum chilense (USA),
Tranzschelia pruni-spinosae on Anemone ranunculoides (Austria),
Uromyces lapponicus on Hedysarum kopetdaghi (Turkmenistan),
U. limonii (UK), U. limonii on Limonium sp. (France), U. pisi on
Euphorbia cyparissias (Germany, Romania), Uromyces sp. on
Euphorbia cyparissias (Switzerland), on Uromyces sp. on Limonium
vulgare (Denmark).
Additional general records (host plants), but without speciic host
fungi: Berberis vulgaris (Austria, Switzerland), Epilobium hirsutum
(Latvia), Euphorbia cyparissias (Austria, Germany, Hungary,
Italy, Slovakia), E. esula (Romania), E. hirsuta (Latvia), Frangula
alnus (Latvia), Hymenoxys hoopesii (≡ Helenium hoopesii) (USA,
AZ), Malus ×micromalus (China, Liaoning), Polygonum aviculare
(Uzbekistan), Ribes uva-crispa var. sativa (= R. grossularia)
(Latvia), R. rubrum (Latvia), Salix amygdalina (Latvia), Scorzonera
hispanica (Germany), Silene vulgaris (Latvia), Sonchus arvensis
(Latvia), Tussilago farfara (Latvia).
Additional specimens examined: Austria, Graubünden, 1000 m, on aecia on
Euphorbia cyparissias (Euphorbiaceae), 13 Jun. 1901, A. Volkart (HBG); Innsbruck,
48
on Aecidium lonicerinum on Lonicera xylosteum (Caprifoliaceae), 19 Aug. 1894,
P. Magnus (HBG); on Coleosporium campanulacearum on Campanula sp.
(Campanulaceae), 29 Aug. 1908, P. Magnus (HBG); on Campanula rapunculoides,
P. Magnus (HBG); near Innsbruck, on aecia on Berberis vulgaris (Berberidaceae),
29 Jun. 1893, P. Magnus (HBG); on Uromyces pisi, Jun., Litschauer & Kreissler,
Krypt. Exs. 2938 (B 700006149); Steiermark, Graz, Botanical Garden, Arboretum,
on Tranzschelia pruni-spinosae on Anemone ranunculoides (Ranunculaceae),
24 Apr. 2008, C. Scheuer 5438 (GZU 21-2008, HAL 2233 F); Tirol, on aecia
on Euphorbia cyparissias, 25 Sep. 1893, P. Magnus (HBG); Tirol, Fulpmes, 7
Aug. 1910, P. Magnus (HBG). Denmark, Lakolk, on Uromyces sp. on Limonium
vulgare (Plumbaginaceae), 21 Jul. 1901, O. Jaap (HBG). Germany, Bavaria,
Bad Reichenhall, on aecia on Euphorbia cyparissias, 14 May 1918, Schoenau
(M-0057481); Bavaria, Kr. Schweinfurt, Gerolzhofen, Grettstadt, Höleinsbach, on
aecia on Scorzonera hispanica (Asteraceae), Jun. 1906, A. Vill, Fungi Bav. 898 (B
700006148); May 1905 (B 700006136); Bavaria, München, on Aecidium ligustri on
Ligustrum vulgare (Oleaceae), 24 Jun. 1882, Allescher (M-0057479); München,
Petersbrunn, on Aecidium cyparissiae on Euphorbia cyparissias, 1893, Allescher
(M-0057480); Bavaria, Oberfranken, Bayreuth, May 1874, ex herb. Körnicke (B
700006147); Brandenburg, Börnicke, 1872, J. Eichelbaum (HBG); 29 May 1910,
ex herb. T. Ohrdruf (B 700006155); Rheinland-Pfalz, Kr. Altkirchen, between
Steneroth and Elkenroth, 26 May 1923, A. Ludwig (B 700006207); Saxony, on
Aecidium falcariae on Falcaria sp. (Apiaceae), 27 May 1893, P. Magnus (HBG);
Westfalen, on Puccinia smilacearum-digraphidis on Polygonatum verticillatum
(Asparagaceae), 9 Jul. 1938, A. Ludwig (B 700006152, B 700006153). Hungary,
Posonii, on aecia on Euphorbia cyparissias, Aug. 1890, J.A. Bäumler (M-0057487).
Italy, Conegliano, on Aecidium euphorbiae on Euphorbia sp., 1879, Spegazzini,
Dec. Mycol. Ital. 57 (B 700006133); Emilia Romagna, Parma, Langhirano, on aecia
on Euphorbia cyparissias, Apr. 1873, G. Passerini, ex herb. Natter (B 700006132);
Meran, on Coleosporium tussilaginis on Petasites sp. (Asteraceae), 2 Nov. 1893, P.
Magnus (HBG). Romania, Muntenia, distr. Dâmboviţta-Băaduleşsti, on Uromyces
pisi, 16 Apr. 1944, T. Săvulescu, Herb. Mycol. Roman. 1342 (M-0057486). Slovakia,
Banská-Štiavnica, on aecia on Euphorbia cyparissias, 2 Jun. 1897, A. Kmet (HBG).
Switzerland, Engadin, on aecia on Berberis vulgaris, 22 Aug. 1888, P. Magnus (B
700006143, HBG); Kt. Wallis, Zermatt, 26 Jul. 1905, O. Jaap, Jaap, Fl. Schweiz
17 (B 700006141); on aecia on Euphorbia cyparissias, Jaap, Fl. Schweiz 15 (B
700006140). USA, California, Berkeley, on Puccinia sp. on Hordeum chilense
(Poaceae), 20 Sep. 1897, P. Magnus (HBG); Colorado, Manitou, on aecia, 22. Jun.
1906, Clements & Clements, Crypt. Format. Colorad. 270 (B 700006137).
Notes: Cladosporium aecidiicola is somewhat reminiscent of the
saprobic C. herbarum, but clearly differs in having conidia which
are often distinctly narrowed towards the base and more frequently
formed in branched chains. Furthermore, the conidiophores are
not distinctly nodulose as in the latter species. Cladosporium
epichloës on Epichloë typhina, which is also characterised by
having subnodulose conidiophores and verruculose conidia, is
morphologically barely distinguishable from C. aecidiicola, but
tentatively maintained as separate species due to clear ecological
differences and the host specialization. Final conclusions about the
afinity of the two taxa have to be based on inoculation experiments.
Oudemans (1919–1924) listed Coleosporium sp., Aecidium
falcariae and A. tussilaginis (= Puccinia poarum) as hosts of C.
aecidiicola, but without any further details, and Lindau (1907)
recorded it on Euphorbia palustris, E. verrucosa, Falcaria
sp., Lonicera xylosteum, Teucrium scorodonia, on the host
fungus Coleosporium sp. on Petasites sp. and on Campanula
rapunculoides.
the genuS Cladosporium
Fig. 12. Cladosporium agoseridis (HAL 1556 F). Fascicle of conidiophores and
conidia in vivo. Scale bar = 10 µm. K. Bensch del.
3. Cladosporium agoseridis U. Braun & Rogerson, Sydowia
47(2): 142. 1995. Figs 12, 13.
Holotype: USA, Utah, Washington Co., northwest side of Pine
Valley Mountain, northeast of Diamond Valley, vicinity of Mud
Spring, on living leaves of Agoseris glauca (Asteraceae), 7 Jun.
1994, C.T. Rogerson (NY). Isotype: HAL 1556 F.
Lit.: Schubert (2005b: 34).
Ill.: Braun & Rogerson (1995: 143, ig. 1), Schubert (2005b: 35, ig.
3, pl. 1, igs A–G).
In vivo: Leaf spots amphigenous, subcircular or somewhat
irregular, 1–8 mm diam or conluent and larger, greyish brown,
often somewhat zonate, surrounded by a narrow, darker margin
or marginal line, necrotic. Colonies amphigenous, punctiform
to subeffuse, brown, somewhat velvety. Mycelium internal,
subcuticular to intraepidermal; hyphae branched, 3–13 μm wide,
septate, often with swellings and constrictions, pale to medium
olivaceous-brown, smooth, walls thickened, forming small to
moderately large aggregations of inlated hyphal cells, 25–100
μm wide, lattened, only few layers deep, cells subglobose to
cylindrical-oblong, 5–15 μm wide, medium olivaceous-brown,
smooth, thick-walled. Conidiophores solitary, in small loose
groups or often loosely fasciculate, arising from internal hyphae
or hyphal aggregations, erumpent through the cuticle, straight
and subcylindrical to somewhat lexuous, geniculate-sinuous,
unbranched, occasionally once branched, 20–90(–110) × (3–)4–
10(–13) μm, 0–4-septate, often somewhat constricted at the basal
septum, often wider and somewhat swollen at the base, up to 15
μm wide, and attenuated towards the apex, pale yellowish, pale
to medium olivaceous-brown or brown, smooth, walls somewhat
thickened, cells sometimes with distinct, clearly delineated
lumen. Conidiogenous cells integrated, terminal and sometimes
intercalary, cylindrical, 14–55 μm long, often somewhat geniculate,
usually with a single or up to three, rarely four conidiogenous loci,
www.studiesinmycology.org
Fig. 13. Cladosporium agoseridis (HAL 1556 F). A. Symptoms. B. Fascicle of
conidiophores. C. Tip of a conidiophore with attached conidium. D. Conidium. E.
Conidium showing surface ornamentation. F. Tip of a conidiophore with still attached
conidia. G. Small fascicle of conidiophores. Scale bars = 10 (C–F), 20 (B, G) µm.
conspicuous, protuberant, obconically truncate to slightly convex,
2–3.5(–4.5) μm diam, dome and surrounding rim relatively lat,
dome not higher than the surrounding rim, somewhat thickened,
darkened-refractive. Conidia catenate, usually in unbranched
chains, occasionally in branched chains, broadly subglobose,
obovoid, ellipsoid-ovoid, subcylindrical or somewhat clavate, (10–)
12–40(–50) × (5–)6–13(–15) μm, 0–1(–3)-septate, pale yellowish,
olivaceous to brown, loosely to densely verruculose or verrucose,
sometimes almost smooth, walls somewhat thickened, lumen
sometimes distinct, clearly separated from the inner wall, apex
rounded or slightly attenuated, hila obconically truncate to slightly
convex, conspicuous, (1.5–)2–3.5(–4.5) μm diam, somewhat
thickened and darkened-refractive; conidia sometimes germinating
but microcyclic conidiogenesis not observed.
Substrate and distribution: On Agoseris glauca; USA.
Notes: Cladosporium agoseridis belongs to a group of leaf-spotting
species forming fasciculate, relatively broad conidiophores, broad
conidiogenous loci and hila and rather large, above all wide conidia.
Other plant pathogenic Cladosporium species on composites
are well distinguished by having quite distinct lesions, narrower
conidiophores [3–7 μm in C. inopinum; (2.5–)3.5–6(–7) μm in C.
praecox], narrower conidia [2.5–6 μm in C. inopinum; 4–7(–8) μm in
C. praecox] and narrower conidiogenous loci and hila [(1–)1.5–2.5
μm in C. praecox]. A record of Cladosporium sp. on Agoseris glauca
from Washington (Shaw 1973) seems to pertain to C. agoseridis.
49
BenSch et al.
Fig. 14. Cladosporium allicinum (CPC 12211). Macro- and micronematous
conidiophores and conidia in vitro. Scale bar = 10 µm. K. Bensch del.
4. Cladosporium allicinum (Fr. : Fr.) Bensch, U. Braun &
Crous comb. nov. MycoBank MB800304. Figs 14–17.
Basionym: Sphaeria allicina Fr., Kongl. Vetensk. Acad. Handl. 38:
247. 1817 : Fr., Syst. Mycol. 2: 437. 1823.
≡ Sphaerella allicina (Fr. : Fr.) Auersw., in Gonn. & Rabenh., Mycol.
Europaea 5–6: 19. 1869.
≡ Davidiella allicina (Fr. : Fr.) Crous & Aptroot, in Aptroot, Mycosphaerella
and its anamorphs: 2. Conspectus of Mycosphaerella. Biodiversity Ser.
5: 30. 2006.
= Hormodendrum hordei Bruhne, in W. Zopf, Beitr. Physiol. Morph. nied. Org.
4: 1. 1894, non C. hordei Pass., 1887.
≡ Cladosporium herbarum δ cerealium f. hordei (Bruhne) Ferraris, Flora
Ital. Crypt., Pars I, Fungi, Fasc.13: 882. 1914.
≡ Cladosporium bruhnei Linder, Bull. Natl. Mus. Canada 97: 259. 1947.
≡ Cladosporium hordei (Bruhne) Pidopl., Gribnaya Flora Grubykh
Kormov: 268. 1953, nom. illeg., homonym, non C. hordei Pass., 1887.
Holotype: Sweden, Skåne, on tip blight of living leaves of Allium
sp. (Amaryllidaceae) (Fr. no. F-09810, UPS-FRIES). Neotype
of Cladosporium bruhnei (designated in Schubert et al. 2007b):
Belgium, Kampenhout, isol. from Hordeum vulgare (Poaceae),
26 June 2005, J.Z. Groenewald (CBS H-19856). Isoneotype: HAL
2023 F. Ex-type cultures: CBS 121624 = CPC 12211, CPC 12212.
= Cladosporium fasciculatum f. gladioli Roum., Fungi Sel. Gall. Exs., Cent. XII,
No. 1158. 1881, nom. nud. [syntypes: e.g., HBG], syn. nov.
= Cladosporium juncicola [“juncicolum”] Niessl, in herb. [M-0057609], syn.
nov.
Lit.: Saccardo (1899: 1076), Linder (1947: 289), Schubert et al.
(2007b: 118–120).
Ill.: Linder (1947: 289, pl. 14, ig. C), Schubert et al. (2007b: 118–
120, igs 9–12).
Exs.: Ellis & Everhart, Fungi Columb. 163 p.p.; Roumeguère, Fungi
Sel. Gall. Exs. 1158.
In vitro: Ascomata pseudothecial, black, supericial, situated on a
small stroma, globose, up to 250 μm diam; ostioles periphysate,
with apical periphysoids present; wall consisting of 3–6 layers
of reddish brown textura angularis. Asci fasciculate, bitunicate,
subsessile, obovoid to broadly ellipsoid, straight to slightly curved,
50
Fig. 15. Cladosporium allicinum (CPC 12211). A. Conidiophore with characteristic
long secondary ramoconidium and complex conidiophore. B. Detail of hila on
secondary ramoconidia. C. Details of prominent ornamentation on conidia. Scale
bars = 2 (B), 5 (C), 10 (A) µm.
8-spored, 65–90 × 16–25 μm; with pseudoparenchymatal cells
of the hamathecium persistent. Ascospores tri- to multiseriate,
overlapping, hyaline, with irregular lumina, thick-walled, straight
to slightly curved, fusoid-ellipsoidal with obtuse basal end, and
acutely rounded apical end, widest near the middle of the apical
cell, medianly 1-septate, not to slightly constricted at the septum,
(20–)25–27(–30) × (5.5–)6–7 μm. Mycelium supericial, hyphae
branched, 1.5–8 μm wide, pluriseptate, broader hyphae usually
slightly constricted at the septa and somewhat swollen, hyaline to
subhyaline, almost smooth to somewhat verruculose or irregularly
rough-walled, sometimes appearing to have a slime coat, walls
unthickened. Conidiophores macronematous, sometimes also
micronematous, arising as lateral or terminal branches from
plagiotropous or ascending hyphae, erect, straight to more or less
lexuous, sometimes geniculate, nodulose, usually with small headlike swellings, sometimes also with intercalary nodules, sometimes
swellings protruding and elongated to one side, unbranched,
occasionally branched, (7–)20–330 μm, sometimes even longer,
(2–)3–5 μm wide, swellings (4–)5–8 μm wide, pluriseptate, not
constricted at the septa, septa sometimes not very conspicuous,
subhyaline to pale brown or pale olivaceous, smooth or somewhat
verruculose, walls unthickened or almost so, more thickened
with age. Conidiogenous cells integrated, usually terminal,
cylindrical with a terminal head-like swelling, sometimes with a
second swelling, 15–40 μm long, proliferation sympodial, with few
conidiogenous loci conined to swellings, up to ive per swelling,
the genuS Cladosporium
Fig. 16. Cladosporium allicinum (F-09810, UPS-FRIES, holotype). Ascus and
ascospores of the teleomorphic state. Scale bar = 10 µm. P.W. Crous del.
loci protuberant, conspicuous, 1–2 μm diam, thickened and
darkened-refractive. Conidia catenate, formed in branched chains,
straight to slightly curved, small terminal conidia subglobose, ovoid
to obovoid or somewhat limoniform, 4–9 × 2.5–3.5 μm [av. ± SD,
6.5 (± 1.5) × 3.1 (± 0.5) μm], aseptate; secondary ramoconidia
and occasionally formed ramoconidia ellipsoid to subcylindrical
or cylindrical, 10–24(–31) × 3–5(–7) μm [av. ± SD, 16.1 (± 4.1)
× 4.1 (± 0.8) μm], rarely up to 40 μm long, 0–1(–3)-septate, very
rarely 5-septate, subhyaline to pale brown or pale olivaceous,
minutely verruculose to verrucose (mostly granulate with some
muricate projections under SEM), walls unthickened or almost so,
apex rounded or slightly attenuated towards apex and base, hila
protuberant, conspicuous, 1–2 μm wide, up to 1 μm high, thickened
and darkened-refractive; microcyclic conidiogenesis occurring.
In vivo: Agreeing with the features in vitro, but mycelium usually
immersed and conidiophores solitary to fasciculate, arising from
immersed hyphae or swollen hyphal cells, stromata usually absent.
Culture characteristics: Colonies on PDA reaching 22–32 mm diam
after 14 d at 25 ºC, olivaceous grey to iron grey, sometimes whitish,
smoke grey to pale olivaceous due to abundant aerial mycelium
Fig. 17. Cladosporium allicinum (CPC 12211). A–B. Macronematous conidiophores. C. Conidial chains. D. Micronematous conidiophore. E. Ascomata of the teleomorphic state
formed on the host. F–G. Asci. Scale bars = 10 (A–B, D, F), 200 (E) µm.
www.studiesinmycology.org
51
BenSch et al.
covering almost the whole colony, with age collapsing becoming
olivaceous grey, occasionally zonate, velvety to loccose, margin
narrow, entire edge, white, glabrous to somewhat feathery, aerial
mycelium sparse to abundant, white, luffy, growth regular, lat to
low convex, sometimes forming few exudates in the colony centre,
sporulating. Colonies on MEA reaching 21–32 mm diam after 14
d at 25 ºC, grey olivaceous, olivaceous grey to dull green or iron
grey, sometimes whitish to pale smoke grey due to abundant aerial
mycelium, olivaceous grey to iron grey reverse, velvety, margin
narrow, entire edge to slightly undulate, white, radially furrowed,
glabrous to slightly feathery, aerial mycelium sparse to abundant,
mainly in the centre, white, luffy, growth convex to raised, radially
furrowed, distinctly wrinkled in the colony centre, without prominent
exudates, sporulating. Colonies on OA reaching 20–32 mm diam
after 14 d at 25 ºC, smoke grey, grey olivaceous to olivaceous grey,
greenish black or iron grey reverse, margin narrow, entire edge,
colourless to white, glabrous, aerial mycelium sparse to abundant,
dark smoke grey, diffuse, high, later collapsed, felty, growth lat,
without prominent exudates, sporulation profuse.
Substrates and distribution: On living and decaying plant and fungal
material, man, air, hypersaline and industrial water; worldwide.
Additional specimens examined: Sine loco et dato (CBS 188.54 = ATCC 11290
= IMI 049638). Australia, New South Wales, Barrington Tops National Park,
isol. from leaves of Eucalyptus stellulata (Myrtaceae), 3 Jan. 2006, B. Summerell,
(CPC 12921). Austria, Zillertal, on dead leaves of Hordeum sp., 9 Aug. 1906, P.
Magnus (HBG), mixed with C. macrocarpum. Belgium, isol. from Quercus robur
(Fagaceae), (CBS 157.82). Czech Republic, Lisen, isol. from Polygonatum
odoratum (Asparagaceae) (CBS 813.71, albino mutant of CBS 812.71). France,
Perpignan, on old leaves of Gladiolus ×gandavensis (Iridaceae), Dec. 1879,
Roumeguère, Fungi Gall. Sel. Exs. 1158 (B). Germany, CBS 134.31 = ATCC 11283
= IMI 049632; Berlin, park, on dead leaves of Hedera helix (Araliaceae), 19 June
1896, P. Magnus (HBG); Nordrhein-Westfalen, Essen, botanical garden, on dead
Nigella damascena (Ranunculaceae), 10 Jul. 2007, N. Ale-Agha (HAL); Mühlheim
an der Ruhr, isol. from industrial water, IWW 727 (CBS 110024); Sachsen-Anhalt,
Halle (Saale), Robert-Franz-Ring, isol. from leaves of Tilia cordata (Tiliaceae),
2004, K. Schubert (CPC 11386). Italy, South Tirol, Marling near Meran, on dead
leaves of Arrhenatherum elatius (Poaceae), 29 Aug. 1911, P. Magnus (HBG);
Seir, on dead leaves of Sesleria sp. (Poaceae), 19 Aug. 1908, P. Magnus (HBG).
Netherlands, isol. from air (CBS 521.68); isol. from Hordeum vulgare (Poaceae),
1 Jan. 2005, P.W. Crous (CPC 12139); isol. from man, skin (CBS 159.54 = ATCC
36948); Amsterdam, isol. from Thuja tincture (CBS 177.71); Geleen, St. Barbara
Ziekenhuis, isol. from man, skin (CBS 366.80, CBS 399.80); isol. from man, sputum,
Aug. 1955 (CBS 161.55). New Zealand, Auckland, Mt. Albert, Harbutt Ave., on dead
leaves of Cortaderia selloana (Poaceae), 23 Sep. 2007, C.F. Hill 2007/2954 (HAL);
Otago, Lake Harris, isol. from Ourisia macrophylla (Plantaginaceae), 30 Jan. 2005,
A. Blouin, Hill 1135 (CPC 11840). Poland, Białowieża national Park, on dead leaves
of Tilia cordata, 27 Oct. 1992, W. Mułenko (LBL). Russia, Moscow region, isol.
from Polyporus radiatus (Polyporaceae), Oct. 1978 (CBS 572.78 = VKM F-405);
St. Petersburg, Botanical Garden of the Komarov Botanical Institute, on necrotic
segments of leaves of Actinidia arguta (Actinidiaceae), 14 Sep. 2009, V.A. Mel’nik
(HAL 2329 F). Slovenia, Ljubljana, isol. from an air conditioning system, 2004,
M. Butala (EXF-680 = CPC 12046); Sečovlje, isol. from hypersaline water from
salterns (reserve pond), 2005, P. Zalar (EXF-389 = CPC 12042). Spain, Ebro Delta,
isol. from hypersaline water from salterns (crystallisation pond), 2004, P. Zalar (EXF594 = CPC 12045). Switzerland, Graubünden, 980 m, on dead leaves of Dianthus
caryophyllus (Caryophyllaceae), 5 Nov. 1900, A. Volkart (HBG); Simplonhospiz,
2010 m, on dead leaves of Homogyne alpina (Asteraceae), 29 Jul. 1905, O. Jaap
(HBG). USA, New Jersey, Vineland, on necrotic parts of leaves of Hedera helix, Apr.
1893, Ellis & Everhart, Fungi Columb. 163 (HBG); New York, Geneva, isol. from
CCA-treated Douglas-ir pole (CBS 115683 = ATCC 66670 = CPC 5101).
Notes: Based on the recently decided abolition of Art. 59 of the
ICBN, the older teleomorph name Sphaeria allicina has priority
over the anamorph name C. bruhnei, and Cladosporium antedates
Davidiella. Therefore, the combination Cladosporium allicinum
(= C. bruhnei) is introduced. This species belongs to the C.
herbarum complex, as already stated by Linder (1947), but differs
52
from C. herbarum s. str. and C. macrocarpum in having consistently
narrower conidia, usually 2.5–5 μm wide, and the conidiophores
often form only a single apical swelling. Although this species
occurs on numerous substrates and is widely distributed, it has
not yet been recognised as a distinct species since it has probably
been interpreted as a narrow variant of C. herbarum. Based on
morphology and DNA sequence data, the CBS strain CBS 177.71
chosen by Prasil & de Hoog (1988) as representative living strain of
C. herbarum, clusters instead with isolates of C. allicinum. The CBS
strain 813.71 is an albino mutant of the latter species as it does not
appear to contain colour pigment. Furthermore, all isolates from
humans treated until now as C. herbarum proved to be conspeciic
with the narrow-spored C. allicinum.
Aptroot (2006) treated Davidiella tassiana (ascospores
17–25 × 6–8.5 μm, RO), the teleomorph name of Cladosporium
herbarum, as a synonym of D. allicina (ascospores 20–27 × 6–7
μm, UPS). However, the teleomorphs of the two species differ in
apical ascospore taper (acutely rounded in D. allicina, but obtusely
rounded in D. tassiana).
5. Cladosporium allii (Ellis & G. Martin) P.M. Kirk & J.G.
Crompton, Pl. Pathol. 33: 320. 1984. Figs 18, 19.
Basionym: Heterosporium allii Ellis & G. Martin, J. Mycol. 1: 100.
1885.
Fig. 18. Cladosporium allii (NY). Fascicle of conidiophores, conidiogenous cells and
conidia in vivo. Scale bar = 10 µm. U. Braun del.
the genuS Cladosporium
Fig. 19. Cladosporium allii (CBS 101.81). A–H. Conidiophores and conidial chains in vitro. Scale bars = 10 µm.
Neotype: USA, New Jersey, on Allium vineale (Amaryllidaceae),
June 1882, Ellis 3608a (NY), selected by Kirk & Crompton, 1984
[the neotype is topotype material (the true type material was
collected in Aug. 1883)].
= Heterosporium ornithogali var. allii-porri Sacc. & Briard, Rev. Mycol. 8: 25.
1886 [holotype: PAD].
≡ Heterosporium allii var. allii-porri (Sacc. & Briard) Sacc., Syll. fung. 4:
488. 1886.
≡ Heterosporium allii-porri (Sacc. & Briard) Nicolas & Aggéry, Rev. Pathol.
Vég. Entomol. Agric. France 14: 197. 1927.
≡ Heterosporium allii-porri (Sacc. & Briard) Arx, Genera Fungi Sporul.
Pure Culture, Ed. 3: 305. 1981, comb. superl.
≡ Cladosporium allii-porri (Sacc. & Briard) Boerema, Verslagen Meded.
Plantenziektenk. Dienst Wageningen 152: 15. 1978 [“1977”].
= (?) Didymellina intermedia Cif., Not. Matatt. Piante 2: 6–7. 1949
(unconirmed).
= Heterosporium allii var. allii-sativi Bontea & Dumitriaş, Rev. Roumaine Biol.,
Sér. Bot., 12(6): 389. 1967 [isotypes: Săvulescu, Herb. Mycol. Roman. 1749,
e.g. IMI 115600, K].
Lit.: Saccardo (1902: 1065), Lindau (1910: 77), CMI Descriptions
of Pathogenic Fungi and Bacteria No. 841 (1986). Kirk (1986a),
Jordan, Burchill & Maude (1990a,b), Jordan, Maude & Burchill
(1990a,b), David (1997: 29–33), Ho et al. (1999: 116–118), Zhang
et al. (2003: 38–40).
Ill.: CMI Descriptions of Pathogenic Fungi and Bacteria No. 841
(1986: igs A–B), Kirk & Crompton (1984: 321, ig. 3), David (1997:
31, ig. 4, 32, ig. 5 A–C), Zhang et al. (2003: 39, ig. 13 and pl. 5,
ig. 3, pl. X, ig. 1).
Exs.: Reliqu. Farlowianae 177; Săvulescu, Herb. Mycol. Roman. 1749.
In vivo: Leaf spots lacking (fruiting on necrotic leaves) or
amphigenous, ellipsoid-fusiform, oblong, with the long axis
www.studiesinmycology.org
parallel to the leaf veins, 5–25 × 2–15 mm, initially yellowish,
ochraceous, later brown, greyish brown or dingy greyish white,
margin indeinite or with a diffuse paler to darker halo. Colonies
amphigenous, punctiform to conluent, dense, medium to dark
brown. Mycelium immersed. Stromata lacking to well-developed,
10–80 μm diam, immersed, brown, composed of swollen hyphal
cells, 3–20 μm diam, thick-walled. Conidiophores solitary or in
small to large, dense fascicles, arising from internal hyphae or
stromata, erumpent, erect, straight, subcylindrical to moderately
geniculate-sinuous or subnodulose, unbranched, 30–120(–160) ×
4–10 μm (up to 600 μm or even longer in culture or moist chamber),
continuous to pluriseptate, uniformly pale olivaceous, olivaceousbrown or ochraceous-brown, smooth to distinctly verruculose, wall
up to 1 μm wide. Conidiophores reduced to conidiogenous cells or
conidiogenous cells integrated, terminal or intercalary, 10–60 μm
long, conidiogenous loci distinctly coronate, protuberant, peg-like,
1–2 per cell, 2–2.5(–3) μm wide, 1–2 μm high. Conidia solitary or
in simple, rarely branched, short chains, formed on relatively long,
thin, neck-like protuberances that eventually form a septum and
turn to peg-like scars after conidial secession, ellipsoid-ovoid,
obovoid, subcylindrical, inally often soleiform, (15–)20–60(–80)
× (5–)7–15(–17) μm, 0–3-septate (in culture and moist chamber
sometimes up to ive septa), barely constricted at septa, septa
often becoming sinuous with age, pale olivaceous-brown to brown,
wall up to 1 μm wide, one-layered, densely verruculose-verrucose,
ends rounded, hila conspicuously coronate, either rather lat or
distinctly protuberant, peg-like, 1.5–2.5(–3) × 1–1.5 μm.
In vitro: Mycelium dimorphic; hyphae narrow, sparingly branched,
1–2 μm wide, septate, septa very pale, not very conspicuous,
hyaline to subhyaline or very pale olivaceous, smooth or almost
53
BenSch et al.
so or somewhat irregularly rough-walled, walls unthickened, giving
rise to micronematous conidiophores. Second type of hyphae wider
and darker, unbranched or sparingly branched, (3–)4–8 μm wide,
with swellings and constrictions especially at septa, swellings up to
10 μm wide, pale to medium olivaceous-brown, smooth or almost
so, walls somewhat thickened, about 0.5 μm wide, giving rise to
macronematous conidiophores. Macronematous conidiophores
solitary, arising terminally or laterally from plagiotropous or ascending
hyphae, erect, straight to somewhat lexuous, subcylindrical,
nodulose, with a single apical swelling or sometimes with additional
nodules at a lower level, unbranched, occasionally branched
towards the apex, 40–240 × 4–8(–9) μm, pluriseptate, occasionally
slightly constricted at septa, pale to medium olivaceous-brown,
smooth or almost so, walls unthickened or only slightly thickened.
Micronematous conidiophores arising terminally and laterally from
hyphae, somewhat lexuous, narrowly cylindrical-oblong or iliform,
non-nodulose or only subnodulose at or towards the apex, 5–100
× 2–3(–4) μm, 0–4-septate, septa not constricted but somewhat
darkened, subhyaline to pale olivaceous, smooth, sometimes
verruculose towards the apex, walls unthickened. Conidiogenous
cells terminal but also intercalary, cylindrical-oblong, sometimes
distinctly geniculate, with subnodulose or nodulose apices and
one or several additional swellings at a lower level, 19–60 μm long,
conidiogenous loci conined to these swellings being situated on
shoulders, 1.5–2.5 μm diam, thickened and darkened-refractive.
Ramoconidia occasionally formed. Conidia numerous, catenate, in
short unbranched or branched chains, with 2–4(–5) conidia in the
unbranched terminal part of the chain, small terminal conidia broadly
obovoid to ellipsoid, 5–13 × (3.5–)4–6(–6.5) μm [av. ± SD, 9.3 (±
2.4) × 5.0 (± 0.9) μm], 0(–1)-septate, intercalary conidia ellipsoid,
ovoid to subcylindrical, 9–17 × 5–7.5 μm [av. ± SD, 13.1 (± 2.2) × 6.1
(± 0.8) μm], 0–1-septate, secondary ramoconidia broadly ellipsoid
to subcylindrical, sometimes obclavate being narrower at the base,
sometimes irregular in outline due to loci being situated on short
lateral outgrowth or lateral shoulders, 14–28(–35) × (4.5–)6–9(10)
μm [av. ± SD, 22.1 (± 5.1) × 7.2 (± 1.3) μm], 0–2(–4)-septate, septa
not very conspicuous, with age becoming curved or sinuous, pale
to medium or even dark olivaceous-brown, verruculose to distinctly
and densely verrucose, thick-walled, due to ornamentation up to
1.5 μm thick, apex rounded or attenuated towards the base, hila
prominent, conspicuous, (1–)1.5–2.5 μm diam, dome quite lat but
rim protuberant, thickened and darkened-refractive; microcyclic
conidiogenesis commonly occurring with conidia giving rise to
nodulose secondary conidiophores; conidia sometimes germinating.
Culture characteristics: Colonies on PDA olivaceous-grey to irongrey, reverse olivaceous-black, velvety to loccose, somewhat
shiny, margins rhizoid, grey-olivaceous, aerial mycelium loccose,
loose to dense, growth lat, without prominent exudates, sporulating.
Colonies on MEA iron-grey to olivaceous-grey, reverse olivaceousblack, velvety to loccose, margins feathery to rhizoid, olivaceousblack, aerial mycelium scattered, loose to dense, loccose, growth
effuse, without prominent exudates, sporulation profuse. Colonies
on OA grey-olivaceous to olivaceous-grey, reverse leaden-grey to
iron-grey, velvety to loccose, margin grey-olivaceous, feathery,
regular, aerial mycelium loose to more dense, loccose, growth lat,
without prominent exudates, sporulation profuse.
Substrates and distribution: On Allium spp. (Amaryllidaceae);
Asia, Caucasus, Europe, North and South America, New Zealand
– Allium ampeloprasum (Canada), A. canadense (USA, MD, MS,
NY, PA, TX, WI), A. cernuum (USA, WY), A. istulosum (China), A.
54
oleraceum (Latvia), A. porrum (France, Netherlands, New Zealand,
UK, USA, PA), A. sativum (Brazil, China, Denmark, India, Romania),
A. schoenoprasum [incl. A. sibiricum] (Norway), A. scorodoprasum
(China), A. stellatum (USA), A. validum (USA, CA, OR), A. vineale
(USA, DE, IL, NJ, TX), Allium spp. (Armenia, Austria, Belgium,
Canada, Czech Republic, Denmark, France, Georgia, Germany,
Greece, Japan, Kazakhstan, Latvia, Norway, Romania, Russia,
UK, Ukraine).
Additional specimens examined: Netherlands, Limburg, Horst, experimental
garden, isol. from Allium porrum, isol. by G.H. Boerema, CBS H-10337 (CBS 101.81
= ATCC 200948 = PD 80/165, reference strain). Norway, Finnmark Co., Loppa,
Vasbotndalen in Øksfjord, on Allium schoenoprasum (A. sibiricum), 22 Aug. 1968,
H.B. Gjaerum (IMI 159610). UK, without locality, on A. porrum, 12 Mar. 1980, A.L.
Smith (IMI 246076); Oxfordshire, 4 Feb. 1981, J.A. Crompton (IMI 255145).
Notes: Allium cepa has occasionally been reported as host of C.
allii. Such records are, however, wrong and undoubtedly pertain to
C. allii-cepae (David 1997). Cladosporium allii-cepae is comparable
with C. allii but easily distinguishable by its much larger conidia. The
record of Kniphoia uvaria (Xanthorrhoeaceae) as host of C. allii by
Oudeman (1919) is very doubtful. Chinese records of C. allii on A.
ascalonicum probably refers to C. allii-cepae. Allium escalonicum
auct. non L. is a name previously applied to shallots. Since in vitro
the conidia of C. allii are shorter, narrower and not soleiform the
species is reminiscent of C. macrocarpum but genetically the two
species are quite distinct (see Fig. 5).
6. Cladosporium allii-cepae (Ranoj.) M.B. Ellis, More
Dematiaceous Hyphomycetes: 337. 1976. Fig. 20.
Basionym: Heterosporium allii-cepae Ranoj., Ann. Mycol. 8: 399.
1910.
Holotype: Serbia, Sumadija, Rebschule von Veliko Oraschje, on
stems of Allium cepa (Amaryllidaceae), June 1905, N. Ranojevic
(BPI 1108762).
= (?) Heterosporium allii var. cepivorum Nicolas & Aggéry, Rev. Pathol. Vég.
Entomol. Agric. France 14: 197. 1927.
= Mycosphaerella allii-cepae M.M. Jord., Maude & Burchill, Trans. Brit. Mycol.
Soc. 86: 392. 1986.
≡ Davidiella allii-cepae (M.M. Jord., Maude & Burchill) Crous & U. Braun,
Mycol. Progr. 2(1): 10. 2003.
Lit.: Saccardo (1913a: 1388), Ellis (1976: 337), Hall & Kavanagh
(1984, 1985), Ellis & Ellis (1985: 305), CMI Descriptions of
Pathogenic Fungi and Bacteria No. 842 (1986), Kirk (1986b),
Jordan, Burchill & Maude (1990a,b), Jordan, Maude & Burchill
(1986, 1990a,b), David (1997: 33–34), Zhang et al. (2003: 40–42).
Ill.: Ellis (1976: 336, ig. 255), Kirk & Crompton (1984: 322, ig. 4,
323, ig. 5), Ellis & Ellis (1985: pl. 129, ig. 1343), CMI Descriptions
of Pathogenic Fungi and Bacteria No. 842 (1986: igs A–B). Shin
(1995: 92: ig. 1 a–d), David (1997: 32, ig. 5 D–G, 35, ig. 6), Kiehr
et al. (1997), Zhang et al. (2003: 41, ig. 14 and pl. 5, ig. 4).
Exs.: Vestergren, Micromyc. Rar. Sel. Praec. Scand. 1798.
In vivo: Leaf spots on living and faded leaves and stems, more
or less elliptical, with the long axis parallel to the leaf veins, up
to 15 × 5 mm, whitish, yellow, ochraceous, later brownish due to
the developing fungal colonies. Colonies mainly hypophyllous,
punctiform to dense, pale to medium brown. Mycelium internal,
hyphae forming loose to dense stromatic aggregations of swollen
hyphal cells, small to expanded, cells subcircular in outline, up
the genuS Cladosporium
Substrates and distribution: On Allium spp. (Amaryllidaceae); Asia,
Europe, North and South America – Allium cepa (Argentina, Brazil,
Canada, Chile, China, Denmark, France, Greece, India, Ireland,
Japan, Korea, Norway, Peru, Serbia, UK, USA, CA, CO, WA), A.
istulosum (China, Korea, Norway).
Additional specimens examined: India, Kashmir, Lalmandi, Agric. Res. Station, on
Allium cepa, 9 Aug. 1957, T.N. Kaul (IMI 69995). Ireland, Dublin, on Allium cepa, 26
May 1976, E.W. Ryne (IMI 228272).
Notes: Cladosporium allii differs from C. allii-cepae by its much
smaller conidia. Zhang et al. (2003) listed Allium ascalonicum
as host of the latter species from China, which is, however, very
probably wrong as A. escalonicum auct. non L. has previously often
been applied to shallots. The record of Allium sibiricum from India,
Kashmir as host of C. allii-cepae (Ellis 1976) is also wrong and
undoubtedly refers to the IMI specimen cited above, in which the
host proved to be A. cepa. Kiehr et al. (1997) recorded this species
from Argentina and summarised its distribution in South America,
including a list of literature references.
7. Cladosporium alneum Pass. ex
Schlechtendalia 14: 56. 2006. Figs 21, 22.
K.
Schub.,
≡ Cladosporium alneum Pass., in herb.
Holotype: Italy, Emilia Romagna, Parma, on living leaves of Alnus
glutinosa (Betulaceae), 1879, G. Passerini (B 700006156).
Lit.: Schubert (2005b: 35–37).
Ill.: Schubert (2005b: 37, ig. 4, pl. 2, igs A–C), Schubert et al.
(2006: 57, ig. 1, pl. 1, ig. A).
Fig. 20. Cladosporium allii-cepae (BPI 1108762). Fascicle of conidiophores,
conidiophores, conidiogenous cells and conidia in vivo. Scale bar = 10 µm. U.
Braun del.
to 20 μm diam, brown, wall somewhat thickened. Conidiophores
solitary to caespitose, loose to dense, erect, straight, subcylindrical
to geniculate-sinuous, usually not torulose-nodulose, at most cells
slightly swollen by constrictions at septa, usually unbranched,
rarely branched, 60–150(–250) × 4–19 μm, pluriseptate, rather
pale to medium olivaceous or brownish, walls thickened, 0.5–
2.5 μm wide, thicker walls in the lower half sometimes distinctly
two-layered, smooth or occasionally faintly rough-walled.
Conidiogenous cells integrated, terminal, 10–60 μm long, straight,
curved to sinuous, but barely geniculate, apex mostly rounded,
sympodial, occasionally enteroblastically proliferating, usually with
1–3 conidiogenous loci per cell, conspicuous, but barely or only
slightly protuberant, 2–5 μm wide, 1–1.5 μm high, periclinal rim not
very evident. Conidia solitary, occasionally in short chains, broadly
ellipsoid-ovoid, soleiform, subcylindrical, 30–90(–120) × 12–20
μm, (0–)1–2(–3)-septate, septa often somewhat curved to sinuous
with age, usually not constricted at septa or only slightly so, very
pale to pale olivaceous, olivaceous-brown or brown, wall thin to
slightly thickened, 0.5–1.5 μm, densely verruculose-verrucose,
ends rounded, sometimes becoming truncate to concave at the
base with age, hila 2–4 μm wide, 1 μm high, not very protuberant,
somewhat darkened-refractive.
www.studiesinmycology.org
Fig. 21. Cladosporium alneum (B 700006156). Symptoms, conidiophores and
conidia in vivo. Scale bar = 10 µm. K. Bensch del.
55
BenSch et al.
Fig. 22. Cladosporium alneum (B 700006156). A. Symptoms. B. Overview, small, loose group of conidiophores. C. Tip of a conidiophore with several somewhat darkened
conidiogenous loci. Scale bars = 10 (C), 20 (B) µm.
In vivo: Leaf spots amphigenous, subcircular, oval to somewhat
irregular in outline, scattered over the whole leaf surface, extending
and often conluent, becoming oblong-irregular, 1–30 mm long,
1–13 mm wide, pale brownish, ochraceous or clay-coloured,
becoming grey-brown or olivaceous-brown by dense fructiication,
mostly without margin, occasionally with a narrow, irregular, pale
to medium reddish brown margin, affected areas turning fragile
and inally forming shot hole symptoms. Colonies amphigenous,
scattered to subeffuse, loosely caespitose, brown, villose.
Mycelium internal, subcuticular to intra-epidermal, sometimes also
external, growing supericially; hyphae unbranched or sparingly
branched, 3–9 μm wide, septate, sometimes slightly constricted
at the septa, pale to medium olivaceous-brown or medium brown,
smooth, walls somewhat thickened, forming swollen hyphal cells,
subglobose, 7–13 μm wide, medium brown or medium olivaceousbrown, smooth, thick-walled. Stromata usually absent, sometimes
developed, small, up to 30 μm diam, composed of swollen hyphal
cells. Conidiophores solitary, in pairs of two or sometimes in loose
groups, arising from swollen hyphal cells, erumpent, sometimes
arising from internal and external hyphae, lateral and terminal,
erect, straight to somewhat lexuous, sometimes slightly geniculatesinuous and subnodulose towards the apex, unbranched, 25–260
× (2–)3–7(–8.5) μm, pluriseptate, pale to usually pale medium
brown or medium brown, paler towards the apex, sometimes
subhyaline, smooth to somewhat asperulate, especially near the
base, walls thickened, one-layered or two-layered, inner wall not
very conspicuous, 0.5–1 μm wide, usually attenuated towards
the apex, sometimes slightly swollen at the base, up to 10 μm
56
wide, protoplasm of the cells somewhat aggregated at the septa,
appearing to be thickened, similar to distoseptation. Conidiogenous
cells integrated, terminal and intercalary, cylindrical-oblong, 9–46
μm long, proliferation sympodial, with several or even numerous
conidiogenous loci, sometimes situated on small lateral shoulders
or crowded at small multilateral swellings but not conined to them,
protuberant, short cylindrical, subdenticulate, (0.5–)1–2(–2.5)
μm diam, thickened, refractive to somewhat darkened. Conidia
catenate, usually in branched chains, subglobose, ovoid, ellipsoidovoid, subcylindrical to cylindrical, 3–23 × 2.5–6 μm, 0–3-septate,
sometimes slightly constricted at the septa, pale brown or
pale olivaceous, sometimes almost subhyaline, smooth, walls
somewhat thickened, apex rounded or with a single or several hila,
slightly attenuated towards the base, hila protuberant, 0.5–2(–2.5)
μm diam, obconically truncate, thickened, refractive to somewhat
darkened; microcyclic conidiogenesis occurring.
Substrate and distribution: On Alnus glutinosa; Italy.
Notes: Cladosporium alneum is morphologically very close to C.
oncobae, described from New Zealand on Oncoba, but differs in
having deviating lesions and conidiophores with somewhat thinner
walls. Since the two species are geographically and ecologically
clearly distinct, we prefer to maintain two separate species. All
other species of Cladosporium s. lat. on Alnus are quite distinct
from C. alneum and proved to belong to C. herbarum (viz., C.
alnicola Corda and C. alnicola Bubák & Vleugel) or Passalora (C.
bacilligerum).
the genuS Cladosporium
truncate, hila protuberant, 3–5 μm diam, periclinal rim only slightly
raised, often not very conspicuous, thickened, darkened.
Substrate and distribution: On Alopecurus geniculatus; USA.
Notes: Cladosporium alopecuri belongs to a group of leaf-spotting
species with fasciculate conidiophores. It is well characterised
by forming usually rather large, dense fascicles, sometimes in
palisade-like layers, arising from often large stromata. These
characters distinguish this species from all other plant pathogenic
species of Cladosporium, including the morphologically otherwise
close C. phlei. Zhang et al. (2003) listed this species on Alopecurus
geniculatus, A. aequalis and Polypogon fugax (= P. higegaweri)
from China, and considered Fusicladium alopecuri Sawada
(nom. inval.), introduced without Latin diagnosis, a synonym of
F. alopecuri Ellis & Everh., which could not be conirmed since
type material of F. alopecuri Sawada could not be traced and the
Chinese collections were not available for a re-examination.
9. Cladosporium angustisporum Bensch, Summerell,
Crous & U. Braun, Stud. Mycol. 67: 17. 2010. Figs 24, 25.
Holotype: Australia, north Queensland, Daintree N.P., isol. from
Alloxylon wickhamii (Proteaceae), coll. B.A. Summerell, isol. P.W.
Crous (CBS H-20423). Ex-type culture: CBS 125983 = CPC 12437.
Fig. 23. Cladosporium alopecuri (NY). Conidiophores and conidia in vivo. Scale bar
= 10 µm. U. Braun del.
8. Cladosporium alopecuri (Ellis & Everh.) U. Braun,
Schlechtendalia 5: 32. 2000. Fig. 23.
Basionym: Fusicladium alopecuri Ellis & Everh., J. Mycol. 4: 53.
1888.
Holotype: USA, Montana, Columbia Falls, on Alopecurus
geniculatus (Poaceae), 20 May 1887, B.T. Galloway (NY).
Lit.: Schubert (2005b: 38).
Ill.: Braun (2000: 33, ig. 2), Schubert (2005b: 38, ig. 5).
In vivo: Symptoms on faded host plants, deinite leaf spots
lacking, but with yellowish ochraceous to brownish, later greyish
brown discolorations. Colonies amphigenous, punctiform,
scattered to dense, brown to blackish brown, later dark greyish
brown. Mycelium internal, immersed, forming moderately
large stromata, olivaceous to olivaceous-brown, occasionally
conluent. Conidiophores in well-developed, usually fairly
large, dense fascicles, sometimes in palisade-like layers,
arising from stromata, erumpent, erect, straight, subcylindrical
to slightly geniculate-sinuous, unbranched, 40–120 × 5–9
μm, 0–1-septate, subhyaline, pale olivaceous to yellowish or
olivaceous-brown, smooth, thin-walled, conidiophores usually
reduced to conidiogenous cells, mostly with a single or only two
conidiogenous loci, conspicuous, 3.5–5 μm diam, thickened,
darkened. Conidia solitary, obovoid, ellipsoid-ovoid, short
cylindrical, 20–40 × 7–13(–15) μm, 0–1-septate, subhyaline, pale
olivaceous-yellowish to pale brown, densely inely asperulateechinulate, apex obtuse, broadly rounded, base obconically
www.studiesinmycology.org
Fig. 24. Cladosporium angustisporum (CBS 125983). Macro- and micronematous
conidiophores, mycelium often forming ropes, ramoconidia and conidial chains in
vitro. Scale bar = 10 µm. K. Bensch del.
57
BenSch et al.
Fig. 25. Cladosporium angustisporum (CBS 125983). A–H. Macronematous conidiophores and conidial chains. Scale bars = 10 µm.
Ill.: Bensch et al. (2010: 21, igs 5−6).
In vitro: Mycelium immersed and supericial; hyphae branched, 1−3
μm wide, septate, mostly not constricted at septa, subhyaline to
olivaceousbrown, smooth to verruculose or irregularly rough-walled,
walls unthickened, sometimes irregular in outline due to swellings
and constrictions, forming expanded hyphal ropes. Conidiophores
solitary, macro- and micronematous, erect or ascending, arising
terminally from ascending or laterally from plagiotropous hyphae,
straight or lexuous, iliform to cylindrical-oblong, non-nodulose,
usually not geniculate, two types of conidiophores, short and
long ones, 22−280 × (1.5−)2−4 μm, pluriseptate, not constricted
at septa, but sometimes irregular in outline due to wider or
narrower parts within the stalk, pale to medium olivaceous-brown
or pale olivaceous, smooth or verruculose at the base, walls
unthickened or slightly thickened. Conidiogenous cells integrated,
mainly terminal, sometimes also intercalary, neither nodulose
nor geniculate, narrowly cylindrical-oblong, 10−27 μm long, with
several loci crowded at the apex, in intercalary conidiogenous cells
loci mainly situated on small lateral denticles just below a septum,
subdenticulate, conspicuous, 1−1.5(−2) μm diam, thickened and
darkened-refractive. Ramoconidia cylindrical, 18−42(−55) μm
long, 0−1-septate, concolouress with tips of conidiophores, base
broadly truncate, 2.5−3 μm wide, unthickened but sometimes
slightly refractive. Conidia catenate, in branched chains, with 1−5
conidia in the terminal unbranched part of the chain, branching in
all directions, small terminal conidia obovoid to narrowly ellipsoid,
3−6.5 × 1.5−2 μm (av. ± SD: 4.9 ± 1.0 × 1.8 ± 0.3), aseptate,
intercalary conidia narrowly ellipsoid, fusiform, (4−)5.5−11.5(−13) ×
(1.5−)2−2.5(−3) μm (av. ± SD: 8.1 ± 2.4 × 2.4 ± 0.4), 0(−1)-septate,
with 1−3 distal hila, secondary ramoconidia ellipsoid to subcylindrical
58
or cylindrical, (6−)7.5−26 × 2−3 μm (av. ± SD: 14.9 ± 6.1 × 2.7
± 0.4), 0−1-septate, not constricted at the median septum, pale
olivaceous or pale olivaceous-brown, smooth, walls unthickened,
somewhat attenuated towards apex and base, with 2−4(−5) distal
hila, hila conspicuous, subdenticulate, 0.5−2 μm diam, thickened
and darkened-refractive.
Culture characteristics: Colonies on PDA attaining 57−76 mm diam
after 1 mo, pale olivaceous-grey to smoke-grey, mouse-grey due
to abundant sporulation, glaucous-grey towards margins, reverse
greenish-black, luffy, margin whitish, feathery, broad, aerial
mycelium abundant, woolly to luffy, covering almost the whole
colony surface, without prominent exudates, sporulating. Colonies
on MEA reaching 45−60 mm diam after 1 mo, smoke-grey, whitish
to pale olivaceous-grey due to abundant aerial mycelium, reverse
iron-grey to pale greenish-grey, velvety to woolly-luffy, margin
colourless to whitish, feathery, regular, aerial mycelium abundant,
dense, luffy, without prominent exudates, sporulation profuse.
Substrate and distribution: On Alloxylon wickhamii; Australia.
Notes: Cladosporium angustisporum belongs to the C.
cladosporioides complex and is morphologically very close to
C. cladosporioides s. str. but differs in having distinctly narrower
conidia, 1.5−3 μm wide. Furthermore, the mycelium is often
aggregated in hyphal ropes, and the conidiophores are dimorphic.
In phylogenetic analyses, C. angustisporum proved to be allied to
C. subuliforme and C. vignae but is distinct and morphologically
and ecologically easily distinguishable (Bensch et al. 2010).
the genuS Cladosporium
10. Cladosporium annonae Nann., Atti Reale Accad.
Fisiocrit. Siena, Ser. 10, 4(1–2): 91. 1929, as “anonae”. Figs
26, 27.
Holotype: Italy, Siena, botanical garden, on living leaves of Annona
sp. (Annonaceae), Aug. 1928, mixed infection with C. herbarum as
secondary invader (SIENA).
Lit.: Saccardo (1972: 1336), Schubert (2005b: 39–40).
Ill.: Schubert (2005b: 39, ig. 6, pl. 2, igs D–F).
Fig. 26. Cladosporium annonae (SIENA). Symptoms, conidiophores and conidia in
vivo. Scale bar = 10 µm. K. Bensch del.
In vivo: Leaf spots amphigenous, irregular in shape and size,
often near to or limited by the midrib, pale to medium brown,
mostly surrounded by a narrow, more or less irregular, medium
to dark brown margin, paler below, becoming fragile with age,
surrounding leaf tissue often somewhat discoloured, yellowish to
olivaceous. Colonies epiphyllous, loosely scattered, caespitose,
brown. Mycelium internal, subcuticular to intraepidermal; hyphae
branched, 2.5–7 μm wide, septate, with swellings and constrictions,
single hyphal cells up to 12 μm wide, pale to medium olivaceousbrown, smooth, walls thickened, forming a loose to somewhat
denser hyphal network, sometimes aggregated. Stromata mostly
absent, sometimes developed, 10–35 μm diam, composed of
swollen hyphal cells, subglobose, 5–8 μm diam, medium dark
olivaceous-brown to brown, smooth, thick-walled, walls up to 1
μm wide. Conidiophores mostly solitary or in small loose groups,
arising from internal hyphae, swollen hyphal cells or stromata,
erumpent through the cuticle, erect, straight to somewhat lexuous,
cylindrical-oblong, usually without swellings, unbranched, 45–
Fig. 27. Cladosporium annonae (SIENA). A. Symptoms. B. Tip of a conidiophore with several somewhat darkened conidiogenous loci. C. Conidiophore and conidia. Scale bars
= 10 (B–C) µm.
www.studiesinmycology.org
59
BenSch et al.
150(–300) × (3.5–)4–7 μm, septate, not constricted at the septa,
medium olivaceous-brown or brown, often somewhat paler at
the apex, smooth, walls thickened, mostly distinctly two-layered,
up to 1 μm wide, often wider or swollen at the base, 7–11 μm,
protoplasm of the cells somewhat aggregated at the septa, so that
the walls and septa appear to be thickened (as in distoseptation).
Conidiogenous cells integrated, terminal and intercalary, cylindrical,
7–43 μm long, proliferation sympodial, with several conidiogenous
loci, sometimes situated on small shoulders, protuberant, almost
lat to short cylindrical, 1–2(–2.5) μm diam, thickened, refractive,
sometimes slightly darkened. Conidia mostly in branched chains,
straight, subglobose, obovoid, ellipsoid, sometimes subcylindrical,
3–15(–19) × (2–)3–4.5(–6) μm, 0–1(–3)-septate, not constricted
at the septa, at irst subhyaline, later pale olivaceous-brown,
smooth, walls somewhat thickened, apex rounded or attenuated
towards the apex and base, hila protuberant, (0.5–)1–2(–2.5) μm
diam, thickened, refractive to somewhat darkened; sporadically
microcyclic conidiogenesis occurring.
Substrate and distribution: On Annona sp.; Italy.
Notes: Cladosporium annonae belongs to a group of leaf-spotting
Cladosporium species. Type material has been examined, but
the observed conidiophores are much shorter than those given in
Nannizzi’s (1929) original description (240–300 μm). Cladosporium
populicola and C. alneum are two somewhat similar leaf-spotting
species that are, however, ecologically and morphologically easily
distinguishable (see keys and Schubert 2005b). Cladosporium
alneum differs from C. annonae in having longer and usually
somewhat wider conidia, 3–23 × 2.5–6 μm, and conidiophores
arising from swollen hyphal cells as well as internal and external
creeping hyphae. The conidiophores of C. populicola, often with
a distinct lumen which is clearly separated from the inner wall, are
sometimes apically branched, possess thicker walls, (0.5–)1–2 μm
wide, and often proliferate enteroblastically. This phenomenon is
visible as a discontinuity in pigmentation and in thickness of the
wall.
11. Cladosporium antarcticum K. Schub., Crous & U.
Braun, Stud. Mycol. 58: 115. 2007. Figs 28–30.
Holotype: Antarctica, King Georg, Arctowski, isol. from the lichen
Caloplaca regalis (Teloschistaceae), 1991, C. Möller, No. 32/12
(CBS H-19857). Isotype: HAL 2024 F. Ex-type culture: CBS 690.92.
Ill.: Schubert et al. (2007b: 116–117, igs 6–8).
In vitro: Mycelium immersed and supericial, dimorphic, branched,
often with short lateral outgrowths, narrow hyphae 1–3 μm wide,
hyaline to subhyaline, thin-walled, hyphae of the second type
wider, 3.5–8(–9) μm, pluriseptate, often somewhat constricted at
the septa, sometimes swollen, pale to dark greyish olivaceous or
olivaceous-brown, smooth or verruculose, thick-walled, sometimes
even two-layered (two distinct wall layers visible), 1(–1.5) μm thick,
hyphae appearing consistently enveloped in polysaccharide-like
material or covered by a slime coat. Conidiophores micronematous
and macronematous, solitary or in loose groups, arising from
plagiotropous or ascending hyphae, terminally or usually laterally.
Macronematous conidiophores erect to somewhat decumbent,
straight to somewhat lexuous or bent, cylindrical, once or several
times slightly to distinctly geniculate towards the apex due to
60
Fig. 28. Cladosporium antarcticum (CBS 690.92). Macro- and micronematous
conidiophores and conidia in vitro. Scale bar = 10 µm. K. Bensch del.
sympodial proliferation, unbranched or once branched, up to 120
μm long, 3–4.5 μm wide, sometimes slightly attenuated towards
the apex, pluriseptate, up to eight septa, occasionally slightly
constricted at the septa, pale to medium or even dark olivaceousbrown or greyish brown, paler towards apices, smooth to somewhat
rough-walled, walls thickened but thinner-walled towards
apices, sometimes slightly swollen at the base, up to 6 μm wide.
Conidiogenous cells integrated, terminal and intercalary, once
or several times slightly to distinctly geniculate, 10–33 μm long,
proliferation sympodial, with several or numerous conidiogenous
loci, at irst terminal, later turning to one side of the stalk and
situated on small lateral shoulders, up to 14 per cell, protuberant,
denticulate, 1–1.5(–2) μm diam, thickened and darkened-refractive.
Micronematous conidiophores as short lateral, peg-like outgrowths
with a single apical scar or somewhat longer, occasionally once
geniculate with several conidiogenous loci at the apex, 2–22 ×
2–3 μm, pale greyish olivaceous, loci denticulate. Ramoconidia
occasionally occurring, cylindrical, up to 30 μm long, 4–5 μm wide,
0–1-septate, concolorous with the tips of conidiophores, with a
broadly truncate, unthickened and not darkened base, without
dome and rim, 2.5 μm wide. Conidia catenate, in branched chains,
straight, small terminal conidia obovoid, limoniform or narrowly
ellipsoid, 4–14 × 2.5–4 μm [av. ± SD, 8.5 (± 3.3) × 3.5 (± 0.6)],
0(–1)-septate, secondary ramoconidia ellipsoid to cylindrical, often
with several or numerous conidial hila crowded at the distal end, up
to 12, 13–30 × 4–5 μm [av. ± SD, 20.1 (± 5.8) × 4.3 (± 0.5) μm],
0–3-septate, sometimes slightly constricted at the median septum,
pale olivaceous-brown or greyish brown, minutely verruculose to
verrucose (granulate under SEM), walls more or less thickened,
rounded or slightly attenuated towards apex and base, hila
protuberant, denticulate, 0.8–1.5(–2) μm diam, thickened and
darkened-refractive; microcyclic conidiogenesis occurring.
Culture characteristics: Colonies on PDA attaining 9 mm diam after
14 d at 25 ºC, greenish olivaceous to grey olivaceous, at the margin
becoming dull green, reverse with a pale olivaceous grey centre
and a broad olivaceous black margin, margin narrow, regular,
entire edge, white, feathery, aerial mycelium sparse but colonies
appearing felty, growth lat with somewhat elevated colony centre,
prominent exudates not formed, sporulation dense, covering almost
the whole colony. Colonies on MEA attaining 12 mm diam after 14
d at 25 ºC, olivaceous grey to iron grey, iron grey reverse, velvety
the genuS Cladosporium
Fig. 29. Cladosporium antarcticum
(CBS 690.92). A. Overview of the
growth pattern on SNA. Note the
very large bulbous cells formed at
the base of different conidiophores.
Other conidiophores sprout from
the agar surface. B. Overview of
conidiophores and conidia. Note
the large distance of the scars on
the conidiophore and the different
stages of conidial formation on
the tips of other conidia. The long
secondary ramoconidia are also
visible, and sparse aerial hyphae.
C. Detail of B with details of the
ornamentation and scars. The
absence of ornamentation at the
apical (spore-forming) end of the
secondary ramoconidium is clearly
visible. D–E. Tubular structures on
conidiophore (D) and secondary
ramoconidium (E). Scale bars = 2
(E), 5 (C–D), 10 (A–B) µm.
Fig. 30. Cladosporium antarcticum (CBS 690.92). A–B. Macronematous conidiophores. C, G. Mycelium enveloped by a polysaccharide-like layer. D, F. Conidia. E. Micronematous
conidiophore. H. Ramoconidium with numerous distal scars. Scale bars = 10 µm.
www.studiesinmycology.org
61
BenSch et al.
to powdery, aerial mycelium sparse, sporulation profuse. Colonies
on OA attaining 4 mm after 14 d at 25 ºC, olivaceous grey, aerial
mycelium sparse, diffuse, growth lat, without prominent exudates,
sporulating.
Substrate and distribution: On the lichen Caloplaca regalis;
Antarctica.
Notes: Two genuine lichenicolous Cladosporium species are
known, viz., C. antarcticum and C. licheniphilum, described on
apothecia of Pertusaria alpina in Russia. The latter species is,
however, quite distinct from C. antarcticum by its subcylindrical
or only slightly geniculate-sinuous, wider conidiophores, 5–8 μm
diam, with numerous characteristically terminal branches and much
shorter, 0–1-septate, smooth conidia, 3.5–13 × 3–7 μm (Heuchert &
Braun 2006). Other Cladosporium species described from lichens,
viz., C. lichenicola, C. arthoniae and C. lichenum are invalid or have
been excluded (see list of excluded Cladosporium species).
12. Cladosporium aphidis Thüm., Oesterr. Landwirtsch.
Wochenbl. 2(43): 505. 1876. Figs 31–33.
Lectotype (designated here): Austria, Klosterneuburg, on
Symphytum oficinale (Boraginaceae), associated with Aphis
symphyti, Aug. 1875, Thümen, Herb. Mycol. Oecon. 484 (B
700006165). Isolectotypes: Thümen, Herb. Mycol. Oecon. 484 (C.
aphidis mixed with C. herbarum).
Fig. 32. Cladosporium aphidis (CBS H-20938). Conidiophores and conidia in vitro.
Scale bar = 10 µm. K. Bensch del.
Epitype (designated here): Germany, Nordrhein-Westfalen,
Essen, botanical garden, supericially on dead carcasses of aphids
on leaves of Echium vulgare (Boraginaceae), 27 June 2006, N. AleAgha (CBS H-20938, HAL). Ex-type culture: CBS 132182 = CPC
13204.
≡ Cladosporium aphidis Thüm., Herb. Mycol. Oecon., Fasc. X, No. 484.
1877.
= Cladosporium aphidis Thüm., Oesterr. Bot. Z. 27: 12. 1877 [syntypes:
Thümen, Mycoth. Univ. 672, e.g., B, HAL, HBG].
≡ Cladosporium aphidis Thüm., Mycoth. Univ., Cent. VII, No. 672. 1877.
= Cladosporium aphidigenum Pass., in herb. (B).
Lit.: Saccardo (1886: 369), Lindau (1907: 830), Ferraris (1912:
351), Lind (1913: 522), Zhang et al. (2003: 43).
Ill.: Zhang et al. (2003: 43, ig. 16).
Exs.: Roumeguère, Fungi Sel. Gall. Exs. 5192 p.p.; Thümen, Herb.
Mycol. Oecon. 484; Thümen, Mycoth. Univ. 672.
Fig. 31. Cladosporium aphidis (B 700006165). Mycelium, conidiophores and conidia
in vivo. Scale bar = 10 µm. U. Braun del.
62
In vivo: Colonies on carcasses of aphids, on leaves and stems
punctiform to effuse, loccose, often overgrowing entire carcasses
and spreading around the aphids, thin to dense, later often
forming colonies on the leaf surface around the carcasses, inally
sometimes spread on the whole leaf, forming supericial colonies
without nutritive contact to the host plant, i.e., entirely supericial,
patches or effuse, loose to dense, springing from germinating
conidia, probably nourishing from the honey dew produced by the
aphids, colonies olivaceous-brown to dark brown. Mycelium in and
the genuS Cladosporium
Fig. 33. Cladosporium aphidis (CBS 132182). A–I. Conidiophores and conidial chains in vitro. Scale bars = 10 µm.
on aphids, on leaves consistently supericial, often forming weblike aggregations of hyphae; hyphae straight to strongly lexuoussinuous, branched, 2–6 μm wide, subhyaline to olivaceous-brown,
wall thin, about 0.5–1 μm, smooth to distinctly rough-walled, later
with swollen hyphal cells, often monilioid, up to 10 μm diam, walls
darker and thicker, up to 1.5 μm, occasionally distinctly two-layered,
occasionally hyphal cells with distinct central lumen. Stromata
lacking, at most with small aggregations of swollen hyphal cells.
Conidiophores solitary, occasionally in loose groups, arising from
supericial hyphae, lateral, occasionally terminal, or emerging
from aggregations of swollen hyphal cells, erect to decumbent,
shape and size very variable, straight, subcylindrical-conical to
strongly geniculate-sinuous, occasionally subnodulose, simple
or branched, occasionally strongly branched, 10–120 × 2–7 μm,
sometimes up to 300 μm long (but differentiation between very
long, branched conidiophores and ordinary hyphae often dificult),
aseptate to pluriseptate throughout, pale olivaceous to medium
olivaceous-brown or brown, wall thin to somewhat thickened,
0.5–1(–1.5) μm, smooth to distinctly rough-walled. Conidiogenous
cells integrated, terminal, occasionally intercalary or pleurogenous,
5–30 μm long, with a single to several conidiogenous loci 1–2 μm
diam, slightly protuberant. Conidia in simple and branched chains,
shape and size variable, subglobose, ellipsoid-ovoid, obovoid,
fusiform, subcylindrical, secondary ramoconidia 8–25 × 3–8.5 μm,
0–3-septate, conidia 3–20 × 2–5 μm, 0–1-septate, subhyaline to
olivaceous-brown, smooth to faintly rough-walled, conidia thinwalled (< 1 μm), walls of secondary ramoconidia somewhat thicker,
0.5–1.5 μm, conidial cells often with distinct lumen that renders
the conidia seemingly thick-walled, ends rounded to attenuated,
hila often somewhat protuberant, denticle-like, 0.5–1.5 μm diam;
germination and microcyclic conidiogenesis occurring.
www.studiesinmycology.org
In vitro: Mycelium sparingly or loosely branched, 1.5−5 μm
wide, septate, often slightly constricted at septa, almost hyaline,
subhyaline or very pale olivaceous-brown, smooth or minutely
verruculose, walls only slightly thickened, cell lumen granular,
guttulate, sometimes aggregated at walls, single hyphal cells
sometimes swollen, bulbous, mainly near or at septa or at the
base of conidiophores, swellings up to 8 μm wide. Conidiophores
solitary, sometimes in pairs of two, macro- and micronematous,
arising terminally or laterally from plagiotropous or ascending
hyphae, erect to ascending, straight or slightly lexuous, iliform or
subcylindrical, non-geniculate or rarely slightly so towards or near
the apex, non-nodulose, unbranched, occasionally once branched,
20−255 × 3−5(−6) μm, septate, not constricted at septa, slightly
attenuated towards the apex, pale to olivaceous-brown, smooth,
base often appears to be verruculose, cell lumen often as in hyphae
but not all conidiophores with granular lumen. Micronematous
conidiophores narrower, shorter and paler, unbranched, 15−72
× 2−3 μm subhyaline to pale olivaceous-brown, smooth, mostly
with a single or two apical conidiogenous loci. Conidiogenous
cells integrated, terminal, sometimes also intercalary, usually
neither geniculate nor nodulose, 11−40 μm long, with a single or
only few (mostly three) apical conidiogenous loci, 1−2 μm diam,
somewhat thickened and darkened-refractive. Ramoconidia often
formed, straight or slightly lexuous, subcylindrical, 19−45(−50)
× (3−)3.5−5(−5.5) μm, 0−1(−3)-septate, not constricted at septa,
concolouress with apices of conidiophores, not or only very slightly
attenuated towards apex and base, base non-cladosporioid.
Conidia numerous, polymorphous, catenate, formed in branched
chains, small terminal conidia subglobose to obovoid, limoniform
or somewhat irregular, 3−5(−6) × 2−3 μm (av. ± SD: 3.8 ± 0.6
× 2.7 ± 0.4), aseptate, rounded at the apex, intercalary conidia
63
BenSch et al.
broadly ovoid, limoniform or ellipsoid, (4−)5−13 × (2.5−)3−4(−5)
μm (av. ± SD: 7.9 ± 2.1 × 3.6 ± 0.6), 0−1-septate, rounded or
slightly attenuated at apex and base, secondary ramoconidia
ellipsoid to subcylindrical, (8−)10−30(−36) × 3−5(−5.5) μm (av.
± SD: 18.1 ± 6.2 × 3.8 ± 0.6), 0−2(−4)-septate, not constricted,
slightly attenuated towards the base, pale olivaceous-brown
to olivaceous-brown, almost smooth to minutely verruculose
or somewhat irregular, walls thin or only slightly thickened,
sometimes cell lumen distinct that renders the conidia seemingly
thick-walled, lumen mostly granular, hila 0.5−2 μm diam,
somewhat thickened and darkened-refractive.
Culture characteristics: Colonies on PDA attaining 47−53 mm after
1 mo, grey-olivaceous to olivaceous or olivaceous-grey due to
profuse sporulation, greenish-grey towards margin, leaden-grey to
olivaceous-black reverse, velvety, margin white, regular, narrow to
somewhat broader, feathery, aerial mycelium at irst almost absent,
diffuse, later dense, covering large parts of the colony, whitish or
pale olivaceous-grey, felty-woolly, growth low convex to sometimes
umbonate, with age few small but prominent exudates formed,
sporulation profuse. Colonies on MEA attaining 16−30 mm after
1 mo, olivaceous-grey to olivaceous, pale olivaceous-grey due to
aerial mycelium, iron-grey reverse, sometimes zonate, velvety or
powdery, margin colourless or white, regular, radially furrowed,
feathery, colony centre often forming a crater like structure,
immersed, aerial mycelium sparse, diffuse or dense, numerous
small prominent exudates formed, appear almost blackish,
sporulation profuse. Colonies on OA attaining up to 63 mm after
1 mo, grey-olivaceous or olivaceous-grey, reverse iron-grey to
grayish-blue, powdery, margin white, glabrous, narrow, aerial
mycelium sparse, diffuse, growth lat, without prominent exudates,
sporulation profuse.
Substrates and distribution: On or associated with aphids; Asia
(China), Europe (Austria, Denmark, Germany, Italy, UK), South
Africa, North America (USA), Central and South America (Brazil,
Peru, Puerto Rico, Virgin Islands), Oceania (Hawaii).
Additional specimens examined: Austria, Klosterneuburg, on Symphytum oficinale
(Boraginaceae) associated with Aphis symphyti, Aug. 1876, Thümen, Mycoth. Univ.
672 (B 700006166; BPI 426119, 426367; HAL); Steiermark, Graz, Botanical Garden
of the Karl-Franzens-University, on Onosma sp. (Boraginaceae), June 1990, M.
Teppner (HAL 2095 F). Brazil, S.P., Piracicaba, on aphids, on leaves of Manihot
utilissima (Euphorbiaceae), 27 Mar. 1933, A.P. Viégas (BPI 425871). Germany,
Bavaria, Bad Kissingen, supericial on leaves of Lonicera xylosteum (Caprifoliaceae),
6 Sep. 1891, P. Magnus (HBG); Berlin, on Aphis sp., on Pyrus communis
(Rosaceae), 29 July 1923, Noack (B 700006168); Berlin, University Garden, on
aphids and supericially on leaves of Euonymus japonicus (Celastraceae), 4 Mar.
1898, P. Magnus (HBG); Brandenburg, on aphids on leaves of Brassica oleracea
(Brassicaceae), 4 Oct. 1904, O. Jaap (B 700006169); Nordrhein-Westfalen,
Duisburg, on leaves of Buddleja davidii (Scrophulariaceae), 2007, N. Ale-Agha
(HAL); Essen, botanical garden, on leaves of Anthyllis barba-jovis (Fabaceae),
associated with aphids, 18 Aug. 2006, N. Ale-Agha (HAL); on Asplundia moritziana
(= Carludvicia plicata) (Cyclanthaceae), 30 Oct. 2008, N. Ale-Agha (HAL); on
leaves of Echium pininana (Boraginaceae), 27 June 2006, N. Ale-Agha (HAL); on
leaves of Erythrochiton brasiliensis (Rutaceae), associated with aphids, 2006, N.
Ale-Agha (HAL); on leaves of Pericallis appendiculata (Asteraceae), associated with
aphids, 18 Aug. 2006, N. Ale-Agha (HAL); on brown scales and deciduous short
shoots at the base of needles of Pinus sp. (Pinaceae), 3 June 2008 and 18 Jul.
2008, N. Ale-Agha (HAL); on leaves of Sonchus congestus (Asteraceae), associated
with aphids, 8 May 2006, N. Ale-Agha (HAL), CPC 13087. Italy, Emilia Romagna,
Parma, on leaves of Populus alba (Salicaceae), associated with aphids, Passerini
(B 700006164), as “C. aphidigenum”; on aphids on leaves of Symphytum oficinale,
Sep., Passerini (B 700006163 A), as “C. aphidigenum”; on aphids on leaves
of Salix sp., Sep., Passerini (B 700006163 B), as “C. aphidigenum”. Portugal,
Coimbra, on leaves of Asclepias consanguinea (Apocynaceae), supericial,
together with other hyphomycetes, F. Moller, Roumeguère, Fungi. Sel. Gall. Exs.
5192 (B 700006637), as C. mollis. Puerto Rico, Trujillo Alto, on Aphis gossypii on
64
Abelmoschus esculentus (Malvaceae), 10 Apr. 1931, R. Faxon & A.S. Mills (BPI
426118). USA, Delaware, Wilmington, on insect carcasses (aphids?), on leaves of
Buddleja sp. (Scrophulariaceae), 8 Nov. 1926, J.F. Adams (BPI 427205), as “C.
heugelianum”; Hawaii, Honolulu, on Aphis maidis on Zea mays (Poaceae), 20 Oct.
1910, H. Marsh (BPI 426115); Virginia, Clark Co., Blandy Exp. Farm, on carcasses
of insects (aphids?), on leaves of Buddleja asiatica, 23 May 1949, C.L. Lefebvre
(BPI 427206), as “C. heugelianum”; Washington, Seattle, University of Washington
campus, 47.6263530, -122.3331440, isol. from chasmothecia of Phyllactinia guttata
(Erysiphales) on leaves of Corylus avellana (Betulaceae), 2 Dec. 2004, D. Glawe,
CPC 11829.
Notes: Conidia of C. aphidis often germinate on the surface of leaves
and form entirely supericial colonies, sometimes quite independent
of infected aphids. Such supericial colonies are often mixed with
C. herbarum which may also germinate and grow supericially. The
conidia in vitro are narrower and somewhat longer.
A single collection on Musca from Hungary proved to belong
to C. fusiforme which was quite surprising. Cladosporium aphidis
differs from the latter species in having mycelium with distinct
bulboid swellings, longer and somewhat wider macronematous
conidiophores, slightly wider conidiogenous loci, wider ramoconidia
and somewhat longer and wider intercalary and secondary
ramoconidia.
13. Cladosporium apicale Berk. & Broome, J. Linn. Soc.,
Bot. 14: 99. 1873, 1875. Figs 34, 35.
Holotype: Sri Lanka (Ceylon), Peradeniya, on leaves of Cycas
circinalis (Cycadaceae), Jan. 1868, G.H.K. Thwaites (K 121544).
= Cladosporium cycadacearum Shambu Kumar et al., Indian Phytopathol.
60(3): 352. 2007 [holotype: GPU Herb. No. KSR 109; isotype: HCIO 47979].
Lit.: Saccardo (1886: 367), Ellis (1976: 332), Pasqualetti et al.
(2005: 108), Schubert (2005b: 40–41).
Ill.: Ellis (1976: 334, ig. 252 A), Schubert (2005b: 42, ig. 7, pl. 3,
igs A–H), Kumar et al. (2007: 353, ig. 2).
In vivo: On leaves, hypophyllous as greyish to blackish patches
or discolorations, irregular in shape, usually extended, covering
large areas of the leaf surface, stretching halfway or right across
the leaves. Colonies hypophyllous, scattered, in small tufts
or very loosely caespitose, erect to decumbent, dark brown or
almost blackish, villose. Mycelium internal, later also external,
growing supericially, creeping; hyphae sparingly branched,
1.5–5 μm wide, septate, often slightly constricted at the septa,
subhyaline to pale brown, smooth, walls slightly thickened.
Stromata mostly substomatal, composed of more or less angular,
polygonal cells, medium to dark brown, smooth, thick-walled.
Conidiophores mostly loosely fasciculate, occasionally solitary,
arising from stromata, emerging through stomata, more or less
erect, straight or almost so, but somewhat lexuous towards the
apex, cylindrical-oblong, subulate, somewhat geniculate-sinuous
to subnodulose near the apex, unbranched or branched, at
the base and, above all, at the apex, 30–360 μm long or even
longer, 6–11(–16) μm wide at the base, (2–)3–5.5 μm wide at
the apex, pluriseptate, medium to usually dark brown or dark
reddish brown, paler towards the apex, smooth or almost so to
often obviously faintly asperulate, thick-walled, often distinctly
two-layered, 0.75–3 μm wide, sometimes swollen at the base,
protoplasm of the cells somewhat aggregated at the septa, so
that the walls and, above all, septa appear to be thickened (as
in distoseptation), occasionally enteroblastically proliferating.
the genuS Cladosporium
Fig. 35. Cladosporium apicale (K 121544). A. Symptoms. B. Overview, fascicle
of conidiophores. C. Conidiophore with numerous conidiogenous loci and small
subglobose conidia. D. Conidiophore with numerous protuberant conidiogenous
loci. E. Conidiophore showing cell structure, with thickened, two-layered walls and
protoplasm aggregated at the septa. F. Conidia showing coronate scar structure. G,
H. Conidia showing cell structure, with paler cavity in the centre. Scale bars = 5 (D,
F), 10 (C, E, G–H), 50 (B) µm.
Fig. 34. Cladosporium apicale (K 121544). Conidiophores and conidia in vivo. Scale
bar = 10 µm. K. Bensch del.
Conidiogenous cells integrated, terminal and intercalary,
somewhat geniculate-sinuous and subnodulose, 6–23 μm
long, proliferation sympodial, cicatrised, with numerous, small
conidiogenous loci, conspicuously protuberant, subdenticulate,
short cylindrical, often situated on small lateral shoulders or
somewhat crowded, obconically truncate, 1–2 μm diam, dome
often not higher than the surrounding rim, thickened, refractive to
somewhat darkened. Conidia catenate, in branched chains, most
of them very small, subglobose, obovoid, limoniform or ellipsoid,
2–11 × 2–4 μm, 0–1-septate, few conidia larger, ellipsoid, fusiform
or subcylindrical, up to 22 μm long, 3–5(–6) μm wide, 1–3-septate,
not constricted at the septa, pale olivaceous to pale or sometimes
medium olivaceous-brown, smooth or almost so, walls somewhat
thickened, in the centre often with a distinct plae lumen,
surrounded by the somewhat darker protoplasm, apex rounded or
with a single to numerous, small hila, slightly attenuated towards
the base, base truncate or obconically truncate, hila protuberant,
short cylindrical, 0.5–2 μm diam, dome and periclinal rim often not
very conspicuous, thickened, refractive to somewhat darkened;
occasionally microcyclic conidiogenesis occurring.
Substrates and distribution: On Cycas spp. (Cycadaceae); Asia –
www.studiesinmycology.org
Cycas circinalis (Sri Lanka), C. revoluta (India, U.P.).
Notes: The coronate (cladosporioid) structure of the conidiogenous
loci and hila in Cladosporium apicale is not very evident when
examined by light microscopy, but has been proven by means of
SEM (see Schubert 2005b, pl. 3, ig. F). Cladosporium cycadis,
known from Asia, Europe and Central America on leaves of
Cycas spp., is well distinguished from C. apicale by its narrower
conidiophores, 4–5 μm wide, and aseptate, rarely 1-septate, smaller
conidia. Type material of C. cycadacearum was not available, but
based on the description, drawing and host given in Kumar et al.
(2007) it is very probable that this species has to be considered a
synonym of C. apicale.
Unconirmed reports of occurrence of C. apicale have been
published by De & Chattopadhyay (1994) from West Bengal, India,
on Swietenia mahogoni (Meliaceae), Zhang et al. (2003: 45–46,
ig. 18) on Allium istulosum and Magnolia grandilora from China
(Sichuan and Yunnan) and Pasqualetti et al. (2005) on dead leaves
of Arbutus unedo.
65
BenSch et al.
Fig. 36. Cladosporium arthrinioides (M-0057465). Conidiophores and conidia in vivo. Scale bar = 10 µm. K. Bensch del.
Fig. 37. Cladosporium arthrinioides (M-0057465). A. Apex of a conidiophore. B. Conidium showing the coronate scar structure. C. Supericial mycelium. D, E. Conidia. Scale
bars = 2 (B), 10 (A, C–E) µm.
66
the genuS Cladosporium
14. Cladosporium arthrinioides Thüm. & Beltr., Nuovo
Giorn. Bot. Ital. 8: 252. 1876. Figs 36, 37.
Lectotype (designated here): Italy, Sicily, Palermo, botanical
garden, on living and wilted leaves of Bougainvillea spectabilis
(Nyctaginaceae), Mar. 1875, V. Beltrani-Pisani (M-0057465).
Isolectotypes: Thümen, Mycoth. Univ. 873 (BPI 426137, HAL,
HBG, M-0057466, MICH).
Lit.: Saccardo (1886: 359), Ferraris (1912), Schubert (2005b: 43–
44).
Ill.: Schubert (2005b: 43, ig. 8, pl. 4, igs A–E).
Exs.: Thümen, Mycoth. Univ. 873.
In vivo: Leaf spots amphigenous, minute, at irst punctiform, later
subcircular, up to 3 mm diam, dark brown to almost blackish, often
near or at the leaf margin, without distinct margin, velvety, occasionally
somewhat raised. Colonies amphigenous, scattered to subeffuse,
loose to often dense, brown, villose or somewhat downy. Mycelium
external, supericial; hyphae branched, 2–6 μm wide, septate,
sometimes slightly constricted at the septa and somewhat swollen, up
to 9 μm wide, subhyaline to pale or medium dirty olivaceous-brown,
smooth or almost so to somewhat asperulate or irregularly roughwalled, walls thickened, protoplasm of the cells granular, sometimes
distinct and clearly separated from the wall, with small oil droplets,
sometimes aggregated at the septa, so that the walls and, above
all, septa appear to be thickened (as in distoseptation), forming a
more or less dense hyphal network. Stromata lacking. Conidiophores
solitary, arising from supericially growing hyphae, terminal and
lateral, often growing like and confusable with hyphae, erect, straight
to lexuous, iliform, unbranched or once branched, non-nodulose,
25–200 × 2–4 μm, pluriseptate, sometimes slightly attenuated at the
septa, pale to medium dirty olivaceous-brown, smooth to somewhat
asperulate or irregularly rough-walled, walls slightly thickened,
sporadically with oil droplets. Conidiogenous cells integrated, mostly
terminal, sometimes also intercalary, narrowly cylindrical, 5–43 μm
long, proliferation sympodial, with a single to several conidiogenous
loci, protuberant, subdenticulate to denticulate, obconically truncate,
0.5–2 μm diam, dome and periclinal rim often not very conspicuous,
thickened and darkened-refractive. Ramoconidia occasionally
formed, subcylindrical, up to 24 μm long, 4–5 μm wide, 1–2-septate,
broadly truncate at the base, up to 2.5 μm wide. Conidia catenate,
in unbranched or branched chains, small terminal conidia globose,
subglobose, ovoid, limoniform, ovoid-ellipsoid or somewhat
irregular, 2–11 × 2–5 μm, 0–1-septate, secondary ramoconidia and
intercalary conidia ellipsoid, narrowly obclavate to subcylindrical,
13–18 × 3–6.5 μm, 0–1(–2)-septate, occasionally distoseptate,
with age larger and more frequently septate, up to 32 μm long,
with up to four septa, subhyaline, pale to medium dirty olivaceousbrown, smooth or sometimes minutely verruculose, walls more or
less thickened, in the centre of the cells often with distinct lumen,
surrounded by the protoplasm, sometimes also with somewhat
refractive oil droplets, ends rounded or attenuated, hila protuberant,
small, obconically truncate, 0.5–1.5(–2) μm diam, dome and rim
often inconspicuous, thickened, refractive, sometimes darkened;
microcyclic conidiogenesis occurring.
hyphae. However, the coronate (cladosporioid) structure of the
conidiogenous loci and hila has been shown by means of SEM
examinations (see Schubert 2005b, pl. 4, ig. B). Cladosporium
heterophragmatis is the only morphologically comparable
species, which is, however easily distinguishable by having
erect to decumbent, creeping conidiophores with only few nonconstricted septa. Ramoconidia are lacking and the conidia are
never distoseptate. Farr et al. (1989) listed C. arthrinioides on
Bougainvillea sp. from Texas, USA.
15. Cladosporium arthropodii K. Schub. & C.F. Hill, Fungal
Diversity 22: 15. 2006. Figs 38–40.
Holotype: New Zealand, Auckland, Glen Funes, University
of Auckland, Tamaki Campus, on Arthropodium cirratum
(Asparagaceae), 1 Jul. 2004, C.F. Hill, No. 1054, mixed infection
with Alternaria sp. (HAL 1828 F). Paratypes: New Zealand, East
Tamaki, Auckland University Campus, on Arthropodium cirratum, 4
Sep. 2003, E.H.C. McKenzie (PDD 78376) and Little Huia, 3 Dec.
1963, J.M. Dingley (PDD 23039). Epitype (selected here): New
Zealand, Auckland, Glen Funes, University of Auckland, Tamaki
Campus, on Arthropodium cirratum, 8 Oct. 2008, C.F. Hill, CBS
H-20939. Ex-epitype culture: CBS 124043 = CPC 16160.
Lit.: Schubert (2005b: 44–46).
Ill.: Schubert (2005b: 45, ig. 9, pl. 5, igs A–G), Braun et al. (2006:
16, ig. 1, 17, pl. 1).
In vivo: Leaf spots amphigenous, oval to oblong-irregular, at irst
visible as small, whitish, shiny discolorations, later forming larger
spots, up to 30 mm long, inally conluent, covering large areas of the
Substrates and distribution: On Bougainvillea spectabilis; Italy.
Notes: Cladosporium arthrinioides supericially resembles
Stenella species, above all due to verruculose supericial
www.studiesinmycology.org
Fig. 38. Cladosporium arthropodii (HAL 1828 F). Fascicles of conidiophores and
conidia in vivo. Scale bar = 10 µm. K. Bensch del.
67
BenSch et al.
Fig. 39. Cladosporium arthropodii (HAL 1828 F). A. Symptoms. B. Fascicle of conidiophores. C. Conidiophores. D. Tip of a conidiophore with still attached conidium. E.
Geniculate conidiophore. F. Conidium showing surface ornamentation. G. Tip of a conidiophore with still attached conidium. Scale bars = 5 (D), 10 (C, E–G), 20 (B) µm.
68
the genuS Cladosporium
Fig. 40. Cladosporium arthropodii (CPC 16160). A–J. Conidiophores and still attached conidia in vitro. Scale bars = 10 µm.
leaves, on the upper leaf surface whitish, whitish grey to somewhat
rose-coloured, shiny, often with slightly rose- to purple-coloured,
irregular discolorations in the centre of pale spots, somewhat zonate,
surrounded by a narrow or broader, irregular margin or halo, yellowish
brown to dark reddish brown, rarely purple or greenish, on the lower
leaf surface darker, greyish to grey-green. Colonies hypophyllous,
scattered, effuse, loose to dense, short caespitose, olivaceous-brown
to brown or even blackish. Mycelium internal, subcuticular; hyphae
branched, 3–5(–7.5) μm wide, septate, pale olivaceous to pale
olivaceous-brown, smooth, walls unthickened or slightly thickened,
sometimes with small swellings and constrictions, often aggregated.
Stromata mostly substomatal, 20–50 μm diam, dense, compact, pale
to medium olivaceous or olivaceous-brown. Conidiophores loosely to
densely fasciculate, arising from stromata, usually emerging through
stomata, occasionally erumpent through the cuticle, erect, straight
to lexuous, cylindrical-oblong, not to somewhat geniculate-sinuous,
unbranched or rarely branched, non-nodulose to subnodulose, 30–
130 × (3–)4.5–8(–10) μm, 0–5-septate, not constricted at the septa,
very pale olivaceous to pale brown, smooth, sometimes somewhat
verruculose near the apex, walls only slightly thickened, not or only
slightly attenuated towards the apex. Conidiogenous cells integrated,
terminal and intercalary, cylindrical, 10–47 μm long, proliferation
sympodial, with a single to few conidiogenous loci, often on small
lateral shoulders, more or less protuberant, 1.5–2.5(–3.5) μm
wide, 0.5–1 μm high, periclinal rim not distinctly raised, thickened,
somewhat darkened. Conidia solitary or in short unbranched chains,
straight to slightly curved, cylindrical-oblong, (10–)20–60 × (5–)7–12
μm, (0–)1–3(–5)-septate, sometimes slightly constricted at the septa,
septa not very conspicuous, pale to pale medium olivaceous-brown,
echinulate (digitate under SEM), walls more or less thickened, apex
usually rounded, base rounded or often somewhat attenuated,
hila more or less protuberant, 1.5–2.5(–3.5) μm diam, somewhat
darkened.
In vitro (description based on OA): Mycelium immersed and
supericial; hyphae branched, 1–7 μm wide, due to swellings and
constriction somewhat irregular in outline, swellings up to 10 μm
www.studiesinmycology.org
wide, sometimes somewhat geniculate-sinuous or zig-zag-like
and therefore hardly distinguishable from conidiophores, septate,
subhyaline to pale olivaceous-brown, often somewhat darker at
the base of conidiophores, smooth, walls unthickened or almost
so. Conidiophores solitary or sometimes in loose groups of 2–3,
arising terminally or laterally from hyphae, erect, straight to
somewhat lexuous, cylindrical-oblong, often slightly to distinctly
geniculate or subnodulose with unilateral shoulders due to
sympodial proliferation or irregular in outline due to swellings and
constrictions, unbranched, occasionally branched, up to 170 μm
long, 4–6 μm wide, pluriseptate, pale to medium olivaceous-brown,
smooth, walls unthickened or only slightly so. Conidiogenous
cells integrated, terminal or intercalary, geniculate-sinuous or
subnodulose with unilaterally swollen shoulders, 10–31 μm long,
mostly with a single conidiogenous locus per cell, loci often not
very prominent, convex but dome and rim not distinctly raised, 1–3
μm diam, somewhat darkened-refractive. Conidia solitary or in very
short unbranched chains, more or less straight, small conidia ovoid,
pyriform or ellipsoid, aseptate, larger conidia ellipsoid to cylindricaloblong, (9–)15–45 (5.5–)6–10 μm (av. ± SD: 29.3 ± 10.2 × 7.9 ±
1.8), sometimes basal cell bulboid or single cells distinctly swollen,
up to 17 μm diam, 0–3-septate, sometimes slightly constricted
at septa, septa usually not very conspicuous, becoming sinuous
with age, pale to medium olivaceous-brown, densely echinulate,
sometimes loosely verrucose, walls thickened, up to 1 μm wide,
slightly attenuated towards the base, hila conspicuous, 1.5–3 μm
diam, thickened and darkened-refractive; sometimes germinating.
Culture characteristics: Colonies on PDA attaining 1–8 mm diam
after 14 d, olivaceous-grey to white due to aerial mycelium, reverse
iron-grey, luffy, margins white, narrow, aerial mycelium loose to
dense, luffy, abundant, without prominent exudates, sparsely
sporulating on the agar blocks. Colonies on MEA 1–4 mm diam,
white to pale greenish-grey, reverse iron-grey, luffy, margins white,
narrow, feathery, aerial mycelium abundant, white, luffy, covering
the whole colony, dense, growth low convex to convex, without
prominent exudates, sparsely sporulating. Colonies on OA 1–4
69
BenSch et al.
mm, iron-grey to olivaceous-grey, whitish due to aerial mycelium,
reverse olivaceous-grey, velvety, aerial mycelium white, loose,
luffy, growth low convex, without prominent exudates, sporulating.
Substrate and distribution: On Arthropodium cirratum; New
Zealand.
Notes: Cladosporium arthropodii is endemic in New Zealand
on Arthropodium cirratum. Cladosporium allii is morphologically
comparable but well distinguished by its consistently fasciculate
conidiophores and much wider conidia. Furthermore, the periclinal rim
of the conidiogenous loci of C. allii is distinctly elongated (ca. 2 μm
high) giving a peg-like appearance. Cladosporium allii-cepae differs in
having much longer conidia, (40–)60–120(–90) μm, and C. victorialis
(Braun & Mel’nik 1997) is quite distinct by its smooth conidia.
16. Cladosporium asperistipitatum U. Braun & K. Schub.,
Schlechtendalia 16: 62. 2007. Fig. 41.
Holotype: USA, California, Contra Costa Co., Orinda, on living
leaves of Aesculus californica (Sapindaceae), 30 Oct. 1932, L.
Bonar (F 1167622).
Ill.: Braun & Schubert (2007: 63, ig. 1).
In vivo: Leaf spots large, usually marginal, 5–30 mm wide, brown,
with somewhat raised margin, occasionally with diffuse purplish to
brown halo. Colonies amphigenous, faintly punctiform to effuse,
dark brown to blackish. Mycelium internal. Stromata lacking or
small, 10–30 μm diam, substomatal to intraepidermal, brown,
composed of swollen hyphal cells, 3–13 μm diam, walls thickened.
Conidiophores solitary or in small fascicles, arising from internal
hyphae or stromatic hyphal cells, erumpent or emerging through
stomata, erect, straight to slightly geniculate-sinuous, unbranched
or occasionally branched, 10–60 × 2.5–9 μm, continuous to septate,
pale olivaceous to medium olivaceous-brown, wall thickened,
up to 2 μm wide, above all in the lower half, and distinctly twolayered. Conidiogenous cells integrated, terminal, 10–30 μm long;
conidiogenous loci (1–)1.5–2(–2.5) μm diam, somewhat thickened,
darkened and protuberant. Conidia catenate, in simple or branched
Fig. 41. Cladosporium asperistipitatum (F 1167622). Conidiophores and conidia in
vivo. Scale bar = 10 µm. U. Braun del.
70
chains, subglobose, ellipsoid-ovoid, fusiform, limoniform to
subcylindrical, 4–30 × 3–7 μm, 0–2(–4)-septate, pale olivaceous
to olivaceous-brown, walls thin to slightly thickened, < 1 μm wide,
faintly to distinctly verruculose, ends obtuse, rounded to attenuated,
apex with 1–3 hila, base with a single hilum, 1–2 μm diam, slightly
protuberant.
Substrate and distribution: On Aesculus californica; USA.
Notes: Cladosporium asperistipitatum is a biotrophic, leaf-spotting
species morphologically resembling C. gentianae which differs,
however, in having quite distinct lesions and somewhat shorter,
mostly 0–1-septate conidia, frequently with distinct lumina, so that
the conidia become seemingly very thick-walled.
17. Cladosporium asperulatum Bensch, Crous & U. Braun,
Stud. Mycol. 67: 21. 2010. Figs 42–44.
Holotype: Portugal, isol. from Protea susannae (Proteaceae),
1 May 2007, P.W. Crous (CBS H-20424). Ex-type culture: CBS
126340 = CPC 14040.
Ill.: Bensch et al. (2010: 22−24, igs 7−9).
In vitro: Mycelium immersed, sparingly supericial; hyphae
unbranched or very sparingly branched, 2−4.5 μm wide, septate,
not constricted at septa, subhyaline to pale or medium olivaceous-
Fig. 42. Cladosporium asperulatum (CBS 126340). Macronematous conidiophores,
ramoconidia and conidial chains in vitro. Scale bar = 10 µm. K. Bensch del.
the genuS Cladosporium
Fig. 43. Cladosporium asperulatum (CBS 126340). A–D. Conidiophores and conidia. E–G. Secondary ramoconidia and conidia formed in branched chains. Scale bars = 10 µm.
brown, smooth to minutely verruculose or irregularly verrucose, walls
unthickened or almost so, sometimes forming ropes. Conidiophores
macro- and micronematous, solitary, arising terminally or laterally
from plagiotropous or ascending and erect hyphae, erect, straight
to slightly lexuous, cylindrical-oblong, sometimes slightly geniculate
towards the apex, non-nodulose, (15−)45−210(−360) × (2−)3−4(−5)
μm, sometimes up to 5 μm wide at the base, unbranched, occasionally
branched, branches below the apex or at a lower level, usually below
a septum, sometimes up to 105 μm long, pluriseptate with 0−12 septa,
not constricted, pale to medium olivaceous-brown, paler towards the
apex and sometimes attenuated, smooth to asperulate or minutely
verruculose, walls slightly thickened; micronematous conidiophores
iliform or narrowly cylindrical-oblong, about 2 μm wide, paler and
narrower, subhyaline or pale olivaceous-brown, mostly with a
single apical scar. Conidiogenous cells integrated, mainly terminal,
cylindrical-oblong, sometimes slightly geniculate-sinuous towards
the apex, 22−38 μm long, smooth or almost so, with 2−4 apical
loci, protuberant, subdenticulate, sometimes situated on peg-like
prolongations, 1−2 μm diam, thickened and darkened-refractive.
Ramoconidia cylindrical-oblong, 15−50 × 3−4 μm, 0(−1)-septate,
concolouress with tips of conidiophores, smooth or almost so, base
broadly truncate, (2.2−)2.5−3(−3.2) μm wide, unthickened. Conidia
catenate, in branched chains, up to 8(−10) conidia in the terminal
unbranched part of the chain, small terminal conidia obovoid,
4.5−7(−8) × 2−3(−3.5) μm (av. ± SD: 5.6 ± 1.0 × 2.6 ± 0.5), intercalary
conidia ovoid, fusiform to ellipsoid, 5−11(−13) × 2.5−3(−4) μm (av.
± SD: 8.0 ± 2.1 × 2.8 ± 0.4), aseptate, secondary ramoconidia
ellipsoid, fusiform, subcylindrical, (7.5−)9−26(−37) × (2.5−)3−4(−5)
μm (av. ± SD: 18.3 ± 6.6 × 3.4 ± 0.6), 0(−1)-septate, very rarely
with a second septum, not constricted at septa, subhyaline to pale
olivaceous-brown, smooth to minutely verruculose or irregularly
rough-walled (LM), under SEM loosely verruculose or surface with
www.studiesinmycology.org
irregularly reticulate structure or embossed stripes probably caused
by diminishing turgor and shriveling of tender conidia, walls slightly
thickened, attenuated towards apex and base, hila protuberant,
subdenticulate, 0.8−2 μm diam, thickened and darkened-refractive;
microcyclic conidiogenesis not observed.
Culture characteristics: Colonies on PDA attaining 48−53 mm
diam after 14 d, olivaceous-grey, iron-grey or grey-olivaceous at
margins, sometimes zonate, reverse leaden-grey, greyish blue to
iron-grey, powdery to luffy or hairy, margin white, narrow, glabrous,
aerial mycelium abundantly formed, dense, luffy and high in colony
centre, growth lat to low convex with somewhat elevated colony
centre, without prominent exudates, sporulation profuse. Colonies on
MEA reaching 45−64 mm diam after 14 d, olivaceous-grey to pale
greenish grey, reverse olivaceous-grey to iron-grey, powdery to luffy,
margin white to smoke-grey, narrow, regular, glabrous to feathery,
sometimes radially furrowed, aerial mycelium abundant, several
prominent exudates formed appearing blackish, sporulation profuse.
Substrates and distribution: On plant material; Asia (India), Europe
(Portugal), North America (USA).
Additional specimens examined: India, isol. from Eucalyptus leaf litter (Myrtaceae),
1 Mar. 2004, coll. W. Gams, isol. P.W. Crous (CBS 126339 = CPC 11158). Cf.
asperulatum: USA, isol. from grape bud, F. Dugan (208 db sci 1 = CBS 113744).
Notes: Cladosporium asperulatum is phylogenetically close to C.
myrtacearum forming a distinct sister clade (see Bensch et al.
2010, ig. 1, part a). Morphologically this species is comparable
with C. subtilissimum, but differs in having 0−12-septate, somewhat
longer conidiophores and narrower conidia (Schubert et al. 2007b).
An isolate from North America (CBS 113744) is morphologically
and phylogenetically slightly distinct from the other two isolates
71
BenSch et al.
1852, Cesati, Klotzsch, Herb. Viv. Mycol. 1787 (HAL). Isolectotypes:
Klotzsch, Herb. Viv. Mycol. 1787 (e.g., HBG, M).
= Cladosporium acaciae Reichert, Bot. Jahrb. Syst. 56: 720. 1921 [holotype:
B 700006135].
= Cladosporium herbarum var. indutum Thüm., Mycoth. Univ., Cent. XVI, No.
1571. 1880 [syntypes: e.g. HBG].
= Cladosporium juglandis Pass., in herb. [B 700006561]
Lit.: Saccardo (1886: 366), Lindau (1907: 813), Ferraris (1912:
337), Zhang et al. (1999b: 37–38; 2003: 50–51).
Ill.: Zhang et al. (1999b: 37, ig. 2; 2003: 50, ig. 24).
Exs.: Ellis & Everhart, Fungi Columb. 789; Ellis & Everhart, N.
Amer. Fungi 650; Klotzsch, Herb. Viv. Mycol. 1787; Marcucci, Unio
Itin. Crypt. VI; Saccardo, Mycoth. Ven. 586; Thümen, Mycoth. Univ.
1571.
Fig. 44. Cladosporium asperulatum (CBS 126340). A–B. Conidiophores and very
young conidia (A). C–D. Conidia and details of scars on a secondary ramoconidium.
E. Conidia with sparse ornamentation. Note the round conidium-initial. F. Whorl
of secondary ramoconidia formed at the tip of a conidiophore. G. Details of
ornamentation showing loosely irregularly reticulate structures. H. Secondary
ramoconidia. I. Overview of a conidiophore with scars on the tip of the conidiophore.
J. Swollen cells at agar surface giving rise to conidiophores. Note the scar on the
root structure. Scale bars = 1 (D), 2 (G), 5 (A, C, E–F, H–J), 10 (B) µm.
and only tentatively assigned to the present species (Bensch et al.
2010, ig. 1, part a).
18. Cladosporium astroideum Ces., Flora 36: 204. 1853
var. astroideum. Fig. 45.
Lectotype (designated here): Italy, on dead stems of Alisma sp.
(Alismataceae) and leaves and stems of Typha sp. (Typhaceae),
72
In vivo: Saprobic. Colonies small, punctiform to pustulate, subcircular
in outline to dendritic, sometimes subeffuse, scattered to conluent,
forming diffuse layers, dark brown to black. Mycelium internal
and external; immersed hyphae forming strands or small to large
stromatic hyphal aggregations, immersed to erumpent, composed
of swollen hyphal cells, 2.5–17 μm diam, supericial hyphae simple
or branched, 3–10 μm wide, septate, thick-walled, 0.5–1.5 μm wide,
at irst subhyaline to pale olivaceous or olivaceous-brown, later pale
to medium dark brown, smooth to verruculose, septate, often with
constrictions at septa, sometimes effecting a monilioid appearance,
cells sometimes with distinct lumen. Conidiophores solitary or
loosely aggregated, caespitose, arising from immersed hyphae,
swollen hyphal cells or stromata, or solitary, arising from supericial
hyphae, lateral, erect to decumbent, straight to curved, subcylindrical
to geniculate-sinuous, rarely subnodulose, simple or occasionally
branched, 10–120 × 2–8 μm, continuous to pluriseptate, pale to
medium dark brown or olivaceous-brown, sometimes paler towards
the tip, smooth- to rough-walled, walls 0.5–1.5 μm wide, sometimes
distinctly two-layered, cells often with distinct, clearly delineated
lumen, so that the conidiophores appear to be very thick-walled.
Conidiogenous cells integrated, terminal, occasionally intercalary,
10–30 μm long, conidiogenous loci distinctly coronate, 1–2.5 μm
diam, protuberant, denticle-like. Conidia solitary or usually in simple
or branched chains, secondary ramoconidia common, broadly
ellipsoid-ovoid, obovoid, fusiform, subcylindrical, (5–)8–35(–40) × (4–
)5–10(–12) μm, (0–)1–4(–6)-septate, often somewhat constricted at
septa, rarely with 1(–2) oblique or longitudinal septa or septa mixed
with a few distosepta, small aseptate terminal conidia subhyaline
to pale olivaceous, septate secondary ramoconidia or intercalary
conidia pale to medium dark brown or olivaceous-brown, smoothto somewhat rough-walled, walls thin to usually thickened, 0.5–2.5
μm, thick walls sometimes distinctly two-layered, cells often with
distinct lumen, ends rounded or somewhat attenuated, hila coronate,
somewhat protuberant, 1–2 μm diam or (1–)1.5–2.5 μm wide and 1
μm high; germination and microcyclic conidiogenesis occurring.
Substrates and distribution: On dead fruits, leaves and stems;
Europe (Germany, Greece, Italy, Slovenia), North Africa (Egypt),
North America (USA, CA, IL, NJ).
Additional specimens examined: Egypt, Alexandria, Gabbarey, on dead stems of
Typha angustifolia (Typhaceae), 1920, G. Maire, Plantes d’Egypte (HBG); El-Tor,
on dead leaves of Dichanthium foveolatum (≡ Andropogon foveolatus) (Poaceae),
Feb. 1903, R. Muschler, Iter sinaicum (HBG). Germany, Berlin, zoo, on dead leaves
of Acer saccharinum (Sapindaceae), 5 Nov. 1871, P. Magnus (HBG); Brandenburg,
Potsdam, Caputh, on dead leaves of Phragmites australis (Poaceae), 20 Dec. 1877,
P. Magnus (B 700006477). Greece, Rhodos, Kahiram, on dry pods of Vachellia
the genuS Cladosporium
Fig. 46. Cladosporium astroideum var. catalinense (F 1320003). Mycelium,
conidiophores, conidia and microcyclic conidiogenesis in vivo. Scale bar = 10 µm.
U. Braun del.
Fig. 45. Cladosporium astroideum var. astroideum (HAL). Mycelium, conidiophores
and conidia in vivo. Scale bar = 10 µm. U. Braun del.
farnesiana (≡ Acacia farnesiana) (Fabaceae), Feb. 1822, Ehrenberg (B 700006135),
holotype of C. acaciae. Italy, Cagliari, botanical garden, on dead stems of Glebionis
coronaria (Asteraceae), 1866, Marcucci, Unio Itin. Crypt. VI (HBG); Emilia
Romagna, Parma, on dead leaves of Juglans regia (Juglandaceae), G. Passerini
(B 700006561); South Tirol, near Bozen, on dead leaves of Quercus pubescens
(Fagaceae), 13 Nov. 1910, W. Pfaff (HBG); Veneto, Treviso, Bosco Montello, on
dead leaves of Castanea sativa (Fagaceae), Sep. 1875, Saccardo, Mycoth. Ven.
585 (B 700006297). Slovenia, Krain (Carniolia), Laibach, on old leaves of Castanea
sativa, 20 Jan. 1877, W. Voss (B 700006289, HBG). USA, Carolina, Aiken, on dead
stems of Zea mays (Poaceae), 1877, H.W. Ravenel, Thümen, Mycoth. Univ. 1571
(HBG); Illinois, on dead leaves of Typha latifolia, 15 Sep. 1993, R.A. Harper (HBG);
New Jersey, Newield, on old fruits of Yucca ilamentosa (Agavaceae), Sep. 1894,
Ellis & Everhart, Fungi Columb. 789 and N. Amer. Fungi 650 (HBG).
Notes: Zhang et al. (1999b) reported this species from China as
causal agent of leaf spots on Sagittaria sagittifolia, which is very
doubtful since C. astroideum is a saprobic fungus.
Cladosporium astroideum var. catalinense U. Braun, var.
nov. MycoBank MB800303. Fig. 46.
Etymology: Variety named after the island from which it was
collected.
Distinguished from var. astroideum by its conidiophores that are
verruculose below, somewhat narrower conidia, 3–8 μm, mostly
verruculose, sometimes with oblique or longitudinal septa.
Holotype: USA, California, Santa Catalina Island, Johnson’s
Landing, on dead stems of Foeniculum vulgare (Apiaceae), 24
Sep. 1920, L.W. Nuttall (F 1320003).
www.studiesinmycology.org
Additional specimen examined: USA, Santa Catalina Island, Isthmus, on necrotic
capsules of Isomeris sp. (Cleomaceae), 25 Sep. 1920, L.W. Nuttall (F 1320000).
Notes: The North American collections are close to C. astroideum,
but differ in various details. We tentatively consider this fungus as
variety of the latter species. A reassessment of the whole complex
of C. astroideum, including cultures and sequence analyses, is
necessary to adequately deine the status of this variety.
19. Cladosporium auriculae (Cooke) J.C. David, Mycol.
Pap. 172: 98. 1997. Fig. 47.
Basionym: Heterosporium auriculae Cooke, J. Roy. Hort. Soc. 27:
380. 1902.
≡ Heterosporium auriculae Cooke, Grevillea 16: 109. 1888, nom. inval.
(ICBN Art. 32.1).
Holotype: UK, without locality, on dead leaves of Primula auricula
(Primulaceae), without collector and date, ex herb. M.C. Cooke
[K(M) 146492)].
Lit.: Massee (1910), Saccardo (1913a: 1384).
Ill.: David (1997: 84, ig. 19 A–D).
In vivo: On necrotic leaves, probably saprobic, leaf spots not
evident. Colonies amphigenous, inely punctiform to subeffuse,
medium brown. Mycelium internal and external. Stromata
lacking. Conidiophores solitary to loosely aggregated, arising
from immersed swollen hyphal cells or supericial hyphae, erect,
straight to lexuous, cylindrical-iliform, neither distinctly geniculatesinuous nor nodulose, unbranched or occasionally branched,
about 100–350 × 3–8 μm, pluriseptate throughout, pale to medium
73
BenSch et al.
Fig. 47. Cladosporium auriculae [K(M) 146492]. Conidiophores, conidiogenous cells
and conidia in vivo. Scale bar = 10 µm. U. Braun del.
brown, often paler towards the apex, thin-walled, up to 1 μm wide,
smooth or almost so to distinctly verruculose. Conidiogenous cells
integrated, terminal, occasionally intercalary, 10–40 μm long,
usually not or barely geniculate, unswollen, but often subdenticulate
by protuberant conidiogenous loci, occasionally slightly swollen
or somewhat geniculate-sinuous, with 1–5 conidiogenous loci,
often crowded at the apex, 1.5–3 μm diam, distinctly coronate.
Conidia catenate, in simple or branched chains, subglobose,
ellipsoid-ovoid, obovoid, fusiform, (5–)9–16 × 3–6 μm, secondary
ramoconidia up to 25 × 8.5 μm, 0(–1)-septate, pale to medium
olivaceous or olivaceous-brown, thin-walled, < 1 μm wide, faintly
to usually distinctly verruculose, sometimes even verrucose, ends
rounded to attenuated, with a single basal and 1–4 terminal hila,
often somewhat protuberant, 1–2.5 μm diam, distinctly coronate.
Ramoconidia and microcyclic conidiogenesis not observed.
Substrate and distribution: On Primula auricula; UK.
Notes: David (1997) compared this species with C. cladosporioides,
which is, however, distinct by having smaller, above all narrower,
and smooth conidia. The verruculose conidia resemble those of C.
allicinum (= C. bruhnei) and C. herbarum, but the two species are
easily distinguishable by having distinctly nodulose conidiophores.
74
Fig. 48. Cladosporium australiense (CBS 125984). Conidiophores, ramoconidia and
conidial chains, mycelium sometimes forming ropes in vitro. Scale bar = 10 µm. K.
Bensch del.
20. Cladosporium australiense Bensch, Summerell, Crous
& U. Braun, Stud. Mycol. 67: 24. 2010. Figs 48, 49.
Holotype: Australia, New South Wales, Douglas Park, S 34º10’50’’
E 150º42’18’’, isol. from Eucalyptus moluccana (Myrtaceae), 2006,
coll. B.A. Summerell, isol. P.W. Crous (CBS H-20425). Ex-type
culture: CBS 125984 = CPC 13226.
Ill.: Bensch et al. (2010: 24−25, igs 10−11).
In vitro: Mycelium immersed and supericial, abundant, hyphae
loosely to densely branched, sometimes anastomosing, iliform
to cylindrical-oblong or thicker hyphae irregular in outline due to
intercalary swellings and constrictions, 1−5 μm wide, septate, often
slightly constricted, subhyaline to pale or medium olivaceousbrown, smooth to verruculose or loosely rough-walled, rugose,
walls unthickened or slightly thickened in wider hyphae, rhizoidlike, sometimes cells swollen, up to 7 μm wide, often forming dense
ropes with hyphae entwined. Conidiophores macronematous,
solitary, arising terminally and laterally from hyphae, erect, slightly
lexuous, cylindrical-oblong, often very long, 48−285 × 3−4(−5)
μm, seta-like, mostly neither geniculate nor nodulose, occasionally
subnodulose and slightly geniculate, unbranched or branched,
the genuS Cladosporium
Fig. 49. Cladosporium australiense (CBS 125984). A–B. Conidiophores and conidial chains. C. Conidiophore with a septate secondary ramoconidium still attached. D. Conidia.
Scale bars = 10 µm.
branches 3−55 μm long, pluriseptate, not constricted at septa, pale
to medium olivaceous-brown, smooth, walls somewhat thickened,
about 0.5 μm wide, several cells with unusual cell structure having
one or few bigger cavities and protoplasm attached at cell walls
or forming distosepta. Conidiogenous cells integrated, terminal,
occasionally intercalary, cylindrical-oblong, neither geniculate
nor nodulose, 6−15(−40) μm long, with 1−4 loci at the apex or
1−3 loci in intercalary cells with loci situated mostly all at more or
less the same level, like a garland, conspicuous, subdenticulate,
1−2 μm diam, somewhat thickened and darkened-refractive.
Ramoconidia occasionally formed, cylindrical-oblong, often hardly
distinguishable from secondary ramoconidia. Conidia catenate, in
branched chains, branching in all directions, up to 2−4(−5) conidia
in the terminal unbranched part of the chain, small terminal conidia
globose, subglobose to obovoid, 3−6 × 2−3 μm (av. ± SD: 4.2 ±
0.9 × 2.5 ± 0.4), aseptate, rounded at the apex, intercalary conidia
ovoid, ellipsoid to subcylindrical, 5−14(−16) × 2.5−3(−4) μm (av. ±
SD: 9.1 ± 3.2 × 3.0 ± 0.4), 0−1-septate, not constricted at septa,
with 1−3 distal hila, secondary ramoconidia ellipsoid, subcylindrical
to cylindrical, (7−)11−25(−27) × 3−4 μm (av. ± SD: 18.5 ± 5.1 ×
3.4 ± 0.3), 0−1(−2)-septate, septum median or often somewhat
in the upper half, not constricted at septa, often with additional
distosepta, cells with one or few small to large cavities giving the
cells a somewhat thick-walled appearance, pale olivaceous to pale
olivaceous-brown, smooth, walls unthickened to slightly thickened,
slightly attenuated towards apex and base, hila conspicuous,
subdenticulate to denticulate, (0.5−)0.8−2 μm diam, thickened and
darkened-refractive; microcyclic conidiogenesis not observed.
Notes: Cladosporium australiense pertains to the C. cladosporioides
complex and is morphologically close to the latter species s. str.,
but genetically clearly distinct, forming a separate clade in sister
position to C. xylophilum and C. verrucocladosporioides, and
differs in having conidiophores and secondary ramoconidia with
one or several bigger cavities and protoplasm attached at cell walls
or forming distosepta. Furthermore, the mycelium usually forms
dense ropes and secondary ramoconidia are 0−1-septate or may
have one or more distosepta.
Culture characteristics: Colonies on PDA attaining 70−75 mm
diam after 14 d, pale olivaceous-grey, grey-olivaceous towards
margins, reverse greyish-blue to iron-grey, loccose to luffy,
margins colourless to white, regular, somewhat feathery, aerial
mycelium abundant, forming loosely to densely loccose or luffy
mats covering the whole colony, smoke-grey to pale olivaceousgrey, growth low convex, without prominent exudates, sporulation
sparse. Colonies on MEA reaching up to 80 mm diam after 14
d, olivaceous-grey to pale olivaceous-grey, glaucous-grey due
to sporulation, reverse iron-grey, velvety to loccose, margins
colourless to white, regular, feathery, aerial mycelium abundant,
densely loccose, growth effuse, radially furrowed, without
prominent exudates, sporulating. Colonies on OA attaining 65−69
mm diam after 14 d, pale olivaceous-grey to pale greenish-grey,
In vitro: Mycelium immersed, rhizoid, aerial mycelium sparsely
formed; hyphae sparingly branched, 4−7 μm wide, septate, not
constricted or often distinctly constricted at septa, medium to dark
brown, paler at the tips, at the base of the conidiophores distinctly
swollen and constricted at septa, 5−11 μm wide, medium to dark
brown, smooth to asperulate, thick-walled, sometimes forming
stromatic hyphal aggregations. Conidiophores macronematous,
solitary or in groups of two or three, rarely four, arising from swollen
hyphal cells or stromatic hyphal aggregations, erect, straight to
slightly lexuous, often quite rigid, seta-like, usually non-nodulose,
sometimes slightly head-like, unilaterally swollen at the apex, not
geniculate, cylindrical-oblong, distinctly attenuated towards the
apex, unbranched, 35−140 μm long, at the base 4−7(−8) μm wide,
at the apex 3−4 μm wide, 1−6-septate, not constricted at septa,
www.studiesinmycology.org
towards margins and few areas grey-olivaceous, with smaller
dots of smoke-grey, reverse pale mouse-grey with smaller dots or
patches of leaden-grey or iron-grey, velvety to loccose, margins
colourless, regular, glabrous, aerial mycelium abundant, dense,
forming expanded mats, loccose to luffy, growth low convex,
without prominent exudates, sporulating.
Substrate and distribution: On Eucalyptus; Australia.
21. Cladosporium basiinlatum Bensch, Crous & U. Braun,
Stud. Mycol. 67: 25. 2010. Figs 50, 51.
Holotype: Germany, on Hordeum vulgare (Poaceae) (CBS
H-20426). Ex-type culture: CBS 822.84.
Ill.: Bensch et al. (2010: 26−27, igs 12−13).
75
BenSch et al.
like. Conidiogenous cells integrated, terminal, cylindrical-oblong,
11−25 μm long, neither nodulose nor geniculate, with 2−3(−4) loci
at the apex, subdenticulate, protuberant, 1.2−2 μm diam, thickened
and darkened-refractive. Ramoconidia absent or only very rarely
formed. Conidia numerous, catenate, in long unbranched or basely
branched chains, up to 9(−11) conidia in the unbranched part,
straight, small terminal conidia and intercalary conidia obovoid,
ovoid to narrowly ellipsoid, 4−6(−7) × (2−)2.5−3 μm, intercalary
conidia (6.5−)7−10(−11) × 2.5−3.5(−4) μm (av. ± SD: 8.4 ± 1.3 ×
3.0 ± 1.3), aseptate, rounded at the apex or attenuated towards
apex and base, secondary ramoconidia ellipsoid to subcylindrical
or cylindrical, 10−23(−32) × 3−4.5(−5) μm (av. ± SD: 15.0 ± 4.7
× 4.0 ± 0.4), mainly 4 μm wide, 0(−1)-septate, not constricted at
the median septum, pale brown to pale olivaceous-brown, distinctly
paler than conidiophores, smooth or almost so, walls unthickened
or almost so, slightly attenuated towards apex and base, hila
protuberant, often broadly truncate at the base, 1−2(−2.2) μm
diam, somewhat thickened and darkenedrefractive; microcyclic
conidiogenesis occasionally occurring.
Fig. 50. Cladosporium basiinlatum (CBS 822.84). Mycelium, stromatic hyphal
aggregations, conidiophores often with foot-like swollen base and conidial chains in
vitro. Scale bar = 10 µm. K. Bensch del.
medium to dark brown, paler towards the tip, smooth to minutely
verruculose, walls thickened, sometimes two-layered, especially
towards the base, about 1 μm wide, often swollen at the base, foot-
Culture characteristics: Colonies on PDA olivaceous-black to
grey-olivaceous due to profuse sporulation and mycelium, reverse
iron-grey to dark leaden-grey, felty-woolly, margin narrow, white,
feathery, aerial mycelium diffuse, loose, luffy, without prominent
exudates, sporulation profuse, mainly in colony centre. Colonies on
MEA greenish olivaceous to grey-olivaceous, reverse olivaceousgrey to iron-grey, powdery to luffy or felty, margin white, feathery,
narrow, without prominent exudates, sporulation profuse. Colonies
on OA olivaceous-grey to dull green, with age olivaceous, reverse
iron-grey to sky-grey, woolly-felty, margin dull green, outermost
Fig. 51. Cladosporium basiinlatum (CBS 822.84). A–D. Young conidiophores arising laterally from hyphae with conidial chains still attached. E–G. Older conidiophores formed
in pairs arising from distinctly swollen hyphal cells or stromatic hyphal aggregations and numerous conidia. H. A single ramoconidium and numerous conidia. I. A conidial chain
composed of intercalary and small terminal conidia. Scale bars = 10 µm.
76
the genuS Cladosporium
margin colourless to white, feathery, aerial mycelium abundant,
felty-woolly, loose to dense, without prominent exudates,
sporulation profuse.
Substrate and distribution: On Hordeum; Germany.
Notes: Cladosporium basiinlatum, which clusters as a sister
to C. lycoperdinum and C. cladosporioides s. lat. (Bensch et al.
2010, ig. 1, part a), is part of the C. cladosporioides complex, but
differs from most species of this group by having mainly aseptate
conidia that are distinctly paler than conidiophores and dark
brown, thick-walled conidiophores with usually foot-like swollen
basal cells. Cladosporium gentianae is the only species which is
morphologically comparable with C. basiinlatum, but its conidia
are somewhat darker, verruculose or irregularly rough-walled, and
0−1(−3)-septate (Lobik 1928, Schubert 2005b).
22. Cladosporium borassi Hasija, Indian Phytopathol. 19:
373. “1966”, 1967, as “borassii”. Figs 52, 53.
Fig. 52. Cladosporium borassi (IMI 109416c). Internal mycelium, fascicle of
conidiophores and conidia in vivo. Scale bar = 10 µm. K. Bensch del.
Holotype: India, Madhya Pradesh, Jabalpur, Howbagh, Coll.
Garden, on living leaves of Borassus labellifer (Arecaceae), 7 Sep.
1964, S.K. Hasija (IMI 109416c).
Lit.: Schubert (2005b: 46–47).
Ill.: Hasija (1967: 375, ig. 2), Schubert (2005b: 47, ig. 10, pl. 4,
igs F–H).
In vivo: Leaf spots amphigenous, at irst as brown pinhead spots
on the upper region of the leaves, later extending, irregular in size,
effuse, fading, turning yellowish to pale brown or pale reddish brown,
surrounded by a narrow, reddish brown to medium brown margin.
Colonies amphigenous, scattered, caespitose, loose to dense,
dark brown to blackish. Mycelium internal, immersed, subcuticular
to intraepidermal; hyphae branched, 2–4 μm wide, septate, often
with small swellings, nodulose, subhyaline to pale olivaceous, walls
slightly thickened. Stromata composed of subglobose, angular or
somewhat irregular cells, 5–10 μm wide, pale to medium brown
or yellowish brown, walls slightly thickened, sometimes forming
extended stromatic layers. Conidiophores in loose to dense fascicles,
arising from stromata, erumpent through the cuticle, erect, straight
to lexuous, sometimes slightly geniculate-sinuous, subnodulose
or nodulose, small swellings up to 6 μm wide, often connected
with conidiogenesis, unbranched, 40–105(–150) × 4–5.5 μm,
pluriseptate, olivaceous-brown to medium brown, somewhat paler
towards the apex, smooth, walls thickened, sometimes distinctly twolayered, up to 1 μm wide, often swollen at the base, up to 8 μm wide.
Conidiogenous cells integrated, terminal or intercalary, proliferation
sympodial, with numerous conidiogenous loci, often situated on small
multilateral swellings, small, often not very conspicuous, truncate to
slightly convex, 0.5–1(–1.5) μm diam or smaller, slightly thickened,
somewhat darkened-refractive. Conidia catenate, in unbranched
or branched chains, straight, subglobose, ellipsoid, subcylindrical,
3–10(–14) × 2.5–6 μm, 0–1-septate, septum more or less median,
sometimes slightly constricted at the septum, pale brown, smooth,
walls slightly thickened, truncate at the base, hila very small, up to 1
μm diam, slightly thickened, somewhat darkened-refractive.
Substrate and distribution: On Borassus labellifer; India.
www.studiesinmycology.org
Fig. 53. Cladosporium borassi (IMI 109416c). A. Symptoms. B. Fascicle of
conidiophores. C. Nodulose conidiophores. Scale bars = 10 (C), 20 (B) µm.
Notes: Cladosporium borassi has subnodulose or nodulose
conidiophores as do species of the C. herbarum complex, but is
quite distinct by its smooth conidia and narrower conidiogenous
loci and hila.
23. Cladosporium bosciae (Sacc.)
Schlechtendalia 14: 58. 2006. Figs 54, 55.
K.
Schub.,
Basionym: Cladosporium compactum f. bosciae Sacc., Ann. Mycol.
8: 340. 1910.
≡ Cladosporium compactum var. bosciae (Sacc.) Sacc., Syll. Fung. 22:
1367. 1913.
Holotype: Eritrea, Barca, Agordat, alt. 640 m, on Boscia
senegalensis (Capparaceae), 23 Feb. 1909, A. Fiori (PAD).
Lit.: Schubert (2005b: 47–48).
Ill.: Schubert (2005b: 49, ig. 11, pl. 6, igs A–E), Schubert et al.
(2006: 59, ig. 2).
In vivo: Leaf spots amphigenous, numerous, punctiform, up to 2
mm wide, often somewhat irregular and limited by larger leaf veins,
77
BenSch et al.
Fig. 54. Cladosporium bosciae (PAD). Conidiophores and conidia in vivo. Scale bar
= 10 µm. K. Bensch del.
grey brown to dark brown or almost blackish, later conluent, then
leaves with a somewhat spotted appearance, spots surrounded by
a narrow, reddish or pale reddish brown, irregular margin, cuticle
of the diseased leaves seemingly destroyed or dislodged, looking
like the damage caused by insects, yellowish ochraceous. Colonies
usually hypophyllous, on the upper leaf surface sparingly developed,
scattered, dense, caespitose, red-brown to dark brown, velvety.
Mycelium internal, immersed; hyphae branched, 3.5–4.5 μm wide,
septate, sometimes slightly constricted at the septa, pale olivaceous
to olivaceous-brown, smooth, walls somewhat thickened, often
somewhat wider and darker at the base of conidiophores. Stromata
intraepidermal composed of subglobose to somewhat angular,
thick-walled cells, 5–12 μm wide, olivaceous-brown, smooth.
Conidiophores solitary or in loose groups, arising from stromata or
from swollen hyphal ropes, erect, more or less straight, cylindricaloblong, unbranched, rarely branched, 35–130 × 4.5–7(–9) μm,
septate, sometimes slightly constricted at the septa, olivaceousbrown, smooth or sometimes faintly asperulate, walls thickened,
sometimes even distinctly two-layered, mostly somewhat paler and
attenuated towards the apex. Conidiogenous cells integrated, at irst
terminal, later intercalary, cylindrical, 10–35 μm long, proliferation
sympodial, with one or only few conidiogenous loci, subdenticulate,
truncate to slightly convex, 1.5–2.5 μm diam, thickened, more or less
darkened-refractive. Conidia in branched chains, polymorphous,
small terminal conidia subglobose, ovoid, obovoid, ellipsoid,
subcylindrical, 4–11 × 2.5–5(–6) μm, 0–1-septate, larger intercalary
conidia ellipsoid, fusiform, cylindrical 10–20 × 3.5–6(–7) μm,
(0–)1–3-septate, ramoconidia and secondary ramoconidia oblong,
ellipsoid-cylindrical, 18–40 × 4.5–8 μm, 1–6(–8)-septate, not to
slightly constricted at the septa, pale olivaceous, olivaceous-brown
to brown, smooth or almost so, sometimes faintly rough-walled,
Fig. 55. Cladosporium bosciae (PAD). A. Conidiophores. B. Chain of conidia. C. Conidia. D, E. Conidia showing cell structure, with paler cavity in the centre of the cells. Scale
bars = 10 (B–E) µm.
78
the genuS Cladosporium
walls slightly to distinctly thickened, in the centre often with distinct
lumen, surrounded by the somewhat darker protoplasm, similar to
distoseptation, apex rounded to attenuated, hila truncate to slightly
convex, 1–2.5 μm diam, thickened, somewhat darkened-refractive;
microcyclic conidiogenesis not observed.
Substrate and distribution: On Boscia senegalensis; Eritrea.
Notes: The biology of Cladosporium bosciae is still unknown, i.e., it is
unclear if this species is biotrophic or saprobic. It is morphologically
well distinguished from all other species of Cladosporium including
the common saprobic species C. herbarum, C. oxysporum and C.
cladosporioides.
24. Cladosporium brassicae (Ellis & Barthol.) M.B. Ellis,
More Dematiaceous Hyphomycetes: 340. 1976. Fig. 56.
Basionym: Cladotrichum brassicae Ellis & Barthol., Trans. Kansas
Acad. Sci. 16: 167. 1899.
≡ Heterosporium brassicae (Ellis & Barthol.) Arx, Genera Fungi Sporul.
Pure Cult., Ed. 3: 305. 1981.
Lectotype (designated here): USA, Kansas, Rooks Co., on old
leaves of Brassica oleracea (Brassicaceae), 22 Apr. 1897, E.
Bartholomew 2399 (NY). Isolectotypes: NY; Ellis & Everhart, N.
Amer. Fung. 3589 (e.g., K, IMI 99694, NY) and Ellis & Everhart,
Fungi Columb. 1274 (e.g. NY).
Lit.: David (1997: 82), Zhang et al. (2003: 55–56).
Ill.: Ellis (1976: 341, ig. 259 A), David (1997: 83, ig. 18; 84, ig. 19
E–G), Zhang et al. (2003: 55, ig. 28).
Exs.: Ellis & Everhart, Fungi Columb. 1274; Ellis & Everhart, N.
Amer. Fung. 3589.
In vivo: Leaf spots amphigenous, but usually more densely fruiting
on the upper leaf surface, patches at irst more or less discrete,
1–2.5 mm diam, circular to ellipsoid, later extending and often
conluent, 1–2 cm diam, covering large areas of the leaf surface,
dark olivaceous to brown, without margin. Colonies amphigenous,
short caespitose, dense, velvety. Mycelium immersed, subcuticular
to intraepidermal; hyphae sparingly branched, 4–8 μm wide, septate,
subhyaline to pale olivaceous, sometimes slightly constricted at
septa, walls smooth and only slightly thickened, forming hyphal
aggregations and stromata. Stromata at irst small, consisting of
only few swollen hyphal cells, later extended but only few layers
deep, swollen hyphal cells 7–13 μm wide, subglobose to somewhat
angular, pale to medium olivaceous or olivaceous-brown, smooth,
thick-walled. Conidiophores in small to large, loose to somewhat
dense fascicles, often effuse, forming extended layers, arising from
swollen hyphal cells or stromata, emerging through stomata or
erumpent through the cuticle, erect, straight to more or less lexuous,
geniculate, once or usually several times, often closely so, nodulose,
but not distinctly nodose, unbranched, occasionally branched, usually
only once at the apex of conidiophores, such branches often being
more like short lateral protuberances, 30–150 × 5–10 μm, 10–12 μm
wide at swellings, 1–4-septate, usually one in the lower third, pale
olivaceous to pale medium olivaceous-brown or sometimes reddish
brown, darker towards the base and paler towards the apex, smooth,
occasionally somewhat rough-walled towards apex or near scars,
walls somewhat thickened, up to 1 μm wide, less thickened towards
the apex. Conidiogenous cells integrated, terminal or intercalary,
geniculate, nodulose, with conidiogenous loci conined to swellings,
www.studiesinmycology.org
Fig. 56. Cladosporium brassicae (NY). Fascicle of conidiophores and conidia in
vivo. Scale bar = 10 µm. K. Bensch del.
often very long, up to 70 μm, proliferation sympodial, with a single or
up to four conidiogenous loci, loci sessile to protuberant, 2–3.5 μm
diam, thickened and somewhat darkened-refractive. Conidia solitary
or catenate, in short, unbranched chains, broadly ovoid, ellipsoid
to cylindrical, straight, (7–)10–32(–40) × (7–)9–16 μm, mainly
0–1-septate, often with a second subsidiary septum, occasionally
3-septate, not to sometimes slightly constricted, concolorous with
conidiophores, coarsely verrucose to echinulate, walls somewhat
thickened, up to 1.5 μm wide including ornamentation, both ends
rounded, hila sessile, apparently somewhat immersed, to slightly
protuberant, 2–3.5 μm diam, thickened and darkened-refractive;
microcyclic conidiogenesis not observed.
Substrates and distribution: On Brassica and Thelypodium spp.
(Brassicaceae), Asia, North America – Brassica oleracea (USA,
KS), Brassica oleracea subsp. gongylodes (= B. caulorapa)
(China), B. rapa (B. campestris) (China), Thelypodium sagittatum
(USA, UT).
Additional specimens examined: USA, Utah, Sevier Co., T 238, R2W, Sec 23, ca. 1
mile north of Glenwood, 5400 f., on Thelypodium sagittatum (Brassicaceae), 25 Apr.
1979, Welsh, Welsh & Moore, det. C.T. Rogerson (NY).
Notes: Cladosporium brassicae was until now only known from type
collections. An additional collection on Thelypodium sagittatum from
North America could be examined and proved to be conspeciic.
Conidia were 1–3-septate and the conidiophores were somewhat
darker, reddish brown but agreed well with the concept of the
species concerned. Therefore, Thelypodium sagittatum is a new
host. Brassica oleracea subsp. gongylodes (= B. caulorapa), B.
rapa (= B. campestris) and Crepidiastrum denticulatum (≡ Ixeris
denticulata) (Asteraceae) have been recorded as hosts from China
(Zhang et al. 2003).
Type material of C. brassicicola Sawada, an invalid name
published without Latin diagnosis (Sawada 1959), could not
be traced. Therefore, the taxonomic status of this species,
described from Taiwan on leaves of Brassica juncea, is uncertain.
79
BenSch et al.
Cladosporium brassicae is distinguished from the latter taxon by
its coarsely verrucose or echinulate, much wider conidia, 10–14
μm wide.
David (1997) classiied the lectotype material as holotype,
which was, however, not correct. Several duplicates (syntypes)
of the original collection had been distributed in exsiccata, and no
holotype was designated in the original publication.
25. Cladosporium buteicola (“buteacola”) Cooke, Grevillea
5(33): 15. 1876. Fig. 57.
Holotype: India, on pods of Butea monosperma (= B. frondosa)
(Fabaceae), 1876, Hobson 113 [K(M) 121548].
Lit.: Saccardo (1886: 353), Ellis (1976: 343).
Ill.: Cooke (1876: pl. 74, ig. 10), Ellis (1976: 343, ig. 260 B).
In vivo: Colonies on pods, effuse or thin patches, olivaceous-brown.
Mycelium internal and external; supericial hyphae branched,
2–10 μm wide, septate, without or sometimes with constrictions at
septa, olivaceous-brown, smooth or almost so, walls thin to slightly
thickened, forming swollen hyphal cells, 3–12 μm diam, sometimes
aggregations of such cells, but true well-developed stromata
lacking. Conidiophores solitary or in small, loose groups, arising
from immersed or supericial hyphae or aggregations of swollen
hyphal cells, erect, straight, lexuous, iliform, barely or only slightly
geniculate-sinuous, unbranched, 20–300 μm long, 4–10 μm wide
below and 2.5–5 μm wide in the upper half, pluriseptate throughout,
pale to medium olivaceous-brown, at irst smooth, later becoming
verruculose, above all in the lower half, walls 0.5–1 μm wide.
Conidiogenous cells integrated, terminal, 10–30 μm long, not or
only slightly geniculate or subdenticulate, with a single or several
conidiogenous loci, distinctly coronate, 1–2 μm diam, somewhat
protuberant. Conidia catenate, in simple or branched chains,
ellipsoid-ovoid, subcylindrical, pyriform, fusiform, small terminal
and intercalary conidia 4–18 × 2–6 μm, 0–1-septate, secondary
ramoconidia 8–30(–55) × 4–8(–10) μm, 0–3(–4)-septate, pale
Fig. 57. Cladosporium buteicola [K(M) 121548]. Mycelium, conidiophores and
conidia in vivo. Scale bar = 10 µm. U. Braun del.
80
olivaceous to olivaceous-brown, smooth to faintly rough-walled,
walls thin to slightly thickened, up to 1 μm wide, ends rounded
to attenuated, hila 1–2 μm diam; germination and microcyclic
conidiogenesis occurring.
Substrate and distribution: On Butea monosperma; India.
Notes: This species is only known from the type collection. It is
morphologically close to C. delicatulum, also described by Cooke
from India, but differs in forming abundant supericial hyphae with
solitary conidiophores. Furthermore, distinct lumina in cells of the
conidiophores and conidia are lacking.
26. Cladosporium caraganae K. Schub., U. Braun & H.D.
Shin, Schlechtendalia 16: 63. 2007. Figs 58, 59.
Holotype: South Korea, Jinju, Gazwa Arboretum, Forestry
Research Institute, on dead but not fallen leaves of Caragana
sinica (Fabaceae), 15 Oct. 2003, H.D. Shin (HAL 2008 F).
Ill.: Braun & Schubert (2007: 64, ig. 2).
In vivo: Leaf spots amphigenous, on the upper leaf surface
subcircular to irregular, about 2 mm diam, grey to blackish, on the
lower leaf surface forming blackish patches, not distinctly limited,
somewhat dendritic towards the margin. Colonies hypophyllous,
effuse, dense, grey to dark olivaceous-brown, velvety. Mycelium
internal, subcuticular to intraepidermal, later also external; hyphae
unbranched or branched, 2–7 μm wide, septate, often slightly to
distinctly constricted at septa, sometimes with swellings, up to 9(–
13) μm wide, subhyaline to pale olivaceous or olivaceous-brown,
smooth, walls mostly thickened, cells often with small oil droplets or
appearing granulated, forming loose to dense hyphal aggregations.
Stromata lacking. Conidiophores solitary, arising from supericial
Fig. 58. Cladosporium caraganae (HAL 2008 F). Conidiophores arising from
supericial hyphae and conidia in vivo. Scale bar = 10 µm. K. Bensch del.
the genuS Cladosporium
Fig. 59. Cladosporium caraganae (HAL 2008 F). A. Supericial hyphal ropes giving rise to conidiophores. B. Details of scar structure on a secondary ramoconidium and conidia.
C. Tip of a conidiophore with smooth, catenate conidia. Scale bars = 10 (B–C), 20 (A) µm.
hyphae, laterally from creeping hyphae or terminally from ascending
hyphae, erect, straight to slightly lexuous, non-nodulose,
sometimes distinctly geniculate towards the apex, unbranched,
occasionally apically branched, 30–200(–250) × 3–6(–7) μm,
septate, occasionally slightly constricted at septa, pale olivaceous
or olivaceous-brown, paler towards the apex, sometimes almost
hyaline, smooth, sometimes slightly rough-walled, walls thickened,
up to 1 μm wide, thinner-walled towards the apex. Conidiogenous
cells integrated, terminal but also intercalar, cylindrical-oblong,
10–55 μm long, sometimes geniculate, proliferation sympodial,
with a single or usually several denticulate conidiogenous loci,
often crowded, protuberant, 1.5–2.5(–3) μm diam, thickened and
somewhat darkened-refractive. Conidia catenate, in unbranched or
branched chains, straight, ellipsoid, fusiform or cylindrical, 5–35(–
55) × (2.5–)3.5–6.5(–7.5) μm, 0–4(–5)-septate, occasionally slightly
constricted at septa, subhyaline, pale olivaceous or olivaceousbrown, smooth, walls unthickened or somewhat thickened, slightly
attenuated towards the base, cells with oil droplets appearing
granular, hila 1–2 μm diam, thickened and darkened-refractive;
microcyclic conidiogenesis occurring.
Substrate and distribution: On Caragana sinica; South Korea.
Notes: Cladosporium caraganae belongs to a group of leaf-spotting
Cladosporium species morphologically close to C. cladosporioides,
C. psoraleae and C. vignae but easily distinguishable from those
species by its 0–4-septate conidia and loose to dense hyphal
aggregations.
27. Cladosporium carpesii Sawada, Bull. Gov. Forest Exp.
Sta. 105: 93. 1958.
Holotype: Japan, Iwate Prefecture, Morioka, on leaves of
Carpesium abrotanoides var. thunbergianum (Asteraceae), 12
Sep. 1947, K. Sawada (herb. Univ. Iwate, Japan).
In vivo: Leaf spots circular to variable in shape, 3–10 cm diam,
greenish yellow, margin indeinite, inally with brownish centre.
Mycelium internal or epidermal. Stromata lacking or only composed
of a few swollen hyphal cells, brown. Conidiophores solitary,
arising from swollen hyphal cells, erumpent through the cuticle,
erect, assurgent to the leaf hairs or creeping on the leaf surface,
lexuous, cylindrical, unbranched, rarely 1–2 times branched, 48–
250 × 3.4–5.8 μm, pluriseptate, not constricted at septa, brown,
smooth to minutely verruculose, thick-walled. Conidiogenous cells
integrated, terminal and intercalary, with a few conidiogenous loci
per conidiogenous cell, conspicuously protuberant, at intercalary
www.studiesinmycology.org
conidiogenous cells somewhat on shoulders, 1.5–2.2 μm wide, 1–2
μm high, darkened, refractive. Conidia catenate, in unbranched
chain, ramoconidia not observed, straight, fusiform, ovoid to
ellipsoid, 8–14 × 4–8 μm, usually aseptate, not constricted at septa,
pale brown to brown, slightly verruculose, apex slightly attenuated,
protuberant, hila 1–1.5 μm diam.
Substrate and distribution: On Carpesium; Japan.
Notes: Type material of C. carpesii has been traced in the herbarium
of the Iwate University, Japan (without acronym). C. Nakashima reexamined the type material and prepared the current description.
28. Cladosporium cassiae-surathensis J.M. Yen, Bull.
Soc. Mycol. France 97(3): 130–131. 1981. Figs 60–62.
Holotype: Singapore, Bukit Timah, on living leaves of Senna
surattensis (≡ Cassia surattensis) (Fabaceae), 29 Mar. 1970, G.
Lim, No. 45 (LAM: Yen Herb., No. 10541).
Lit.: Braun & Freire (2004: 222), Schubert (2005b: 49–51).
Ill.: Yen (1981: 130, ig. 1), Schubert (2005b: 50–51, igs 12–13, pl.
7, igs A–F).
In vivo: Leaf spots amphigenous, subcircular, oval-oblong to
irregular, 1–8 mm wide, sometimes covering large areas of the leaf
surface, small spots brown, larger ones with a pale brown centre,
surrounded by a narrow, somewhat raised, dark brown, reddish
brown to blackish margin, spots somewhat paler on the lower leaf
surface. Colonies amphigenous, punctiform, scattered, loosely
caespitose, brown, not velvety. Mycelium internal, subcuticular to
intraepidermal, later also external, growing supericially; hyphae
sparsely branched, 3–4 μm wide, septate, not constricted at the
septa, pale brown or somewhat darker at the base of conidiophores,
smooth, walls slightly thickened, often single cells swollen, up to 8
μm. Stromata subcuticular to intraepidermal, small, up to 25 μm
wide, lat, only few layers deep, composed of swollen, subcircular
to somewhat angular, thick-walled cells, 5–9 μm diam, pale
brown to medium brown, sometimes even dark brown, smooth.
Conidiophores solitary arising from internal and external hyphae
or swollen hyphal cells, or in small, loose fascicles (up to 10)
arising from stromata, erumpent through the cuticle, erect, straight
to somewhat lexuous, cylindrical, unbranched, rarely branched,
20–100 × 3–5 μm, septate, not constricted at the septa, pale brown
to mostly medium or even dark brown, somewhat paler towards the
apex, smooth, sometimes slightly rough-walled, walls thickened,
usually distinctly two-layered, up to 1 μm wide, often swollen at
81
BenSch et al.
Fig. 60. Cladosporium cassiae-surathensis. Original drawing from Yen (1981). A–D. Conidiophores. E–H. Conidia. I–J. Germinating conidia. K. Uredospores parasitised by
germ tubes of conidia.
Fig. 61. Cladosporium cassiae-surathensis (HAL). Conidiophores and conidia in vivo. Scale bar = 10 µm. K. Bensch del.
82
the genuS Cladosporium
Fig. 62. Cladosporium cassiae-surathensis (HAL). A. Symptoms on leaves of
Chamaecrista sp. B. Fascicle of conidiophores. C. Tip of a conidiophore with still
attached conidia. D. Fascicle of conidiophores, conidiophores with percurrent,
enteroblastic proliferations. E. Conidiophore with terminal conidiogenous cell with
numerous, small conidiogenous loci crowded near the apex. F. Conidia. Scale bars
= 5 (C), 10 (D–F), 50 (B) µm.
the base, up to 8 μm wide, often enteroblastically proliferating.
Conidiogenous cells integrated, terminal or intercalary, cylindrical,
5–16 μm long, proliferation sympodial, cicatrised, with numerous
small conidiogenous loci, often crowded near the apex, slightly
convex, up to 1 μm diam, more or less thickened, not to slightly
darkened-refractive. Ramoconidia ellipsoid to subcylindrical, 9–14
× 3–4.5 μm, mostly 1-septate, darker, concolorous with the tips of
conidiophores, walls somewhat thicker as in conidia, with mostly
several conidiogenous loci, base broadly truncate, 2 μm wide,
unthickened. Conidia catenate, in unbranched or branched chains,
straight to slightly curved, subglobose, ovoid, ellipsoid-ovoid,
3–11 × 2–4 μm, 0–1-septate, not constricted at the septa, very
pale to pale brown, smooth, walls slightly thickened, ends more
or less rounded, hila truncate to slightly convex, up to 1 μm diam,
thickened, not to slightly darkened.
Substrates and distribution: On Senna and Chamaecrista spp.
(Fabaceae), Asia, South America – Chamaecrista sp. (Brazil),
Senna surattensis (Singapore).
Additional specimen examined: Brazil, State of Ceará, Cascavel County, Preaoca
district, on Chamaecrista sp., 30 Aug. 2003, F. Freire (HAL).
Notes: The present description is mainly based on a single collection
from Brazil on leaves of Chamaecrista sp. (Cassia s. lat.) that
agreed well with the original description of C. cassiae-surathensis
www.studiesinmycology.org
Fig. 63. Cladosporium chalastosporoides (CBS 125985). Conidiophores, conidia in
long unbranched or dichotomously branched chains and microcyclic conidiogenesis
in vitro. Scale bar = 10 µm. K. Bensch del.
except for somewhat longer conidiophores, formed singly as well
as in small loose fascicles arising not only from external hyphae
but also from internal hyphae, swollen hyphal cells and from small
stromata as well as microcyclic conidiogenesis observed by Yen
(1981). Yen (1981) also noted that the germinating conidia may
occasionally penetrate germ pores of uredospores of Uredo
cassiae-surathensis occurring on the same leaves.
29. Cladosporium chalastosporoides Bensch, Crous & U.
Braun, Stud. Mycol. 67: 27. 2010. Figs 63–65.
Holotype: South Africa, Western Cape Province, Jonkershoek
Nature Reserve, isol. from fruiting bodies of Teratosphaeria proteaearboreae (Teratosphaeriaceae) on leaves of Protea nitida [= P.
arborea] (Proteaceae), 4 Jan. 2007, P.W. Crous (CBS H-20427).
Ex-type culture: CBS 125985 = CPC 13864.
Ill.: Bensch et al. (2010: 28−29, igs 14−16).
In vitro: Mycelium immersed and supericial; hyphae unbranched
or loosely branched, 1−3 μm wide, septate, not constricted at
septa, pale to pale medium olivaceous-brown, almost smooth
to usually minutely verruculose, walls unthickened or almost
so. Conidiophores solitary, macronematous, arising terminally
and laterally from hyphae, erect, straight to somewhat lexuous,
cylindrical-oblong, sometimes with constrictions, attenuations and
swellings which give the conidiophores an irregular appearance,
often slightly to distinctly geniculatesinuous, usually once,
83
BenSch et al.
Fig. 64. Cladosporium chalastosporoides (CBS 125985). A–B. Conidiophores and conidia. C–D. Conidial chains. Scale bars = 10 µm.
unbranched or once branched, below the apex or at a lower
level, 30−80 × (2−)2.5−3.5(−4) μm, 1−4-septate, not constricted
at septa, medium olivaceous-brown, smooth, walls unthickened
or only very slightly thickened, occasionally slightly attenuated
towards the apex. Conidiogenous cells integrated, mainly terminal
but also intercalary, cylindrical, often geniculatesinuous, 6−20 μm
long, 1−2(−4) loci at or towards the apex, sometimes situated on
small lateral prolongations, subdenticulate to denticulate, 1−1.5 μm
diam, central dome and periclinal rim not very conspicuous, loci
lat, somewhat thickened and darkened-refractive. Ramoconidia
formed, hardly distinguishable from secondary ramoconidia,
up to 24 μm long, base 2−3 μm wide, broadly truncate. Conidia
catenate, at the base of the chain or intercalary once or twice
mostly dichotomously branched, formed in long, unbranched
chains of up to 18 conidia, small terminal conidia narrowly obovoid
to subcylindrical, 5−9 × 2−2.5 μm (av. ± SD: 7.3 ± 1.1 × 2.1 ±
0.2), aseptate, intercalary conidia fusiform to subcylindrical, 7−14
× 2−3 μm (av. ± SD: 9.4 ± 1.5 × 2.5 ± 0.3), 0(−1)-septate, with 1−2
distal hila, small terminal and intercalary conidia subhyaline, often
distinctly paler compared with secondary ramoconidia, secondary
ramoconidia fusiform to subcylindrical, 10−19 × (2−)2.5−4 μm (av.
± SD: 15.3 ± 2.4 × 3.1 ± 0.5), 0−1(−)2-septate, not constricted at
septa, pale to pale medium olivaceous-brown, smooth or almost
so, walls more or less unthickened, slightly attenuated towards
apex and base and occasionally constricted in the middle, hila
conspicuous, truncate, 0.8−1.8 μm diam, central dome and
periclinal rim not very prominent, neither with LM nor SEM,
thickened and darkened-refractive; microcyclic conidiogenesis very
often occurring with conidia forming secondary conidiophores.
Culture characteristics: Colonies on PDA attaining 42−49 mm diam
after 14 d, olivaceous-grey to iron-grey, olivaceous-black towards
margins, reverse iron-grey to olivaceous-black, loccose to luffyFig. 65. Cladosporium chalastosporoides (CBS 125985). A–B. Examples of
elongated conidia and chains. C. Branch formation on conidiophores. D. Branched
conidiophore, ramoconidia and conidia. E. Elongated conidia. Note the broad areas
of connection between the spores. F. Overview of a large cell mass that gives rise
to conidiophores and spores. G. Detail of a cell mass showing meristematic growth.
H. Differentiation of fungal cells into a cell mass. Scale bars = 5 (A–E), 10 (G–H),
50 (F) µm.
84
the genuS Cladosporium
felty, margins crenate, very narrow, white, feathery, aerial mycelium
abundant, dense, covering most of colony surface, loccose to luffyfelty, growth effuse with elevated colony centre, without exudates,
sporulation sparse. Colonies on MEA attaining 38−56 mm diam after
14 d, pale olivaceous-grey to olivaceous-grey, reverse olivaceous-grey,
velvety to loccose, margins regular, white, glabrous, aerial mycelium
abundant, covering the whole surface, dense, loccose, growth effuse,
radially furrowed, without exudates, sporulation sparse. Colonies
on OA reaching 38−45 mm diam after 14 d, olivaceous-grey to pale
olivaceous-grey, zonate, grey-olivaceous towards margins, colony
centre with dots of pale greenish grey aerial mycelium, reverse irongrey to leaden-grey, velvety to loccose, margin regular to undulate,
narrow, glabrous, white, aerial mycelium abundant, covering the whole
surface, loccose, lat, without exudates, not sporulating.
Substrate and distribution: On fruiting bodies of Teratosphaeria
proteae-arboreae; South Africa.
Notes: The narrow, fusiform conidia of C. chalastosporoides formed
in long, mostly unbranched chains remind one of Chalastospora
(Pleosporales), above all its type species C. gossypii (Simmons
2007, Crous et al. 2009a). However, based on SEM examinations
of the conidiogenous loci and hila, which proved to be coronate
(cladosporioid), as well as its phylogenetic position as sister of C.
hillianum it became evident that this species is part of Cladosporium
s. str. (Bensch et al. 2010).
30. Cladosporium chamaeropis (Unamuno) K. Schub.,
Schlechtendalia 14: 63. 2006. Figs 66, 67.
Basionym: Cladosporium fasciculare f. chamaeropis Unamuno,
Trab. Secc. Cienc. Nat. Congr. Assoc. Progr. Cienc. Oporto 1921:
60. 1922.
Holotype: Spain, near Oriedo, on leaves of Chamaerops humilis
(Arecaceae), May 1921, P. Unamuno (MA 06416).
Lit.: Schubert (2005b: 51–53).
Ill.: Schubert (2005b: 53, ig. 14, pl. 6, igs F–I), Schubert et al.
(2006: 64, ig. 3, pl. 1, igs D–E).
In vivo: Leaf spots amphigenous, at irst small, subcircular-oval to
somewhat oblong, later extending and conluent, oblong-irregular,
covering large areas of the leaf surface, mainly at the tips of leaves,
pale brown or fading, turning pale clay-coloured in the centre,
surrounded by a narrow to wide irregular margin, dark brown or
almost blackish, surrounding leaf tissue discoloured, brownish.
Colonies amphigenous, scattered to sub-effuse in the pale centre,
loosely caespitose, in tufts, brown. Mycelium internal, intraepidermal;
hyphae sparingly branched, 2–4 μm wide, septate, subhyaline to pale
brown, smooth, walls not or only slightly thickened. Stromata usually
well-developed, compact, substomatal to intraepidermal, 10–30 μm
diam, several layers deep, composed of somewhat angular swollen
hyphal cells, 4–10 μm wide, brown to olivaceous-brown, smooth,
thick-walled. Conidiophores mostly in loose to somewhat denser
fascicles, few to numerous, arising from stromata, rarely solitary
arising from swollen hyphal cells, mostly emerging through stomata
or erumpent through the cuticle, erect, straight to somewhat lexuous,
cylindrical-oblong, sometimes slightly geniculate-sinuous towards the
apex, usually without swellings, unbranched or rarely once branched,
20–125 × 4–6.5 μm, septate, not constricted at the septa, pale to
medium olivaceous-brown or brown, sometimes paler towards
www.studiesinmycology.org
Fig. 66. Cladosporium chamaeropis (MA 06416). Fascicles of conidiophores
emerging through stomata or erumpent through the cuticle and conidia in vivo. Scale
bar = 10 µm. K. Bensch del.
the apex, smooth or almost so, walls thickened, often distinctly
two-layered, up to 1 μm wide. Conidiogenous cells integrated,
terminal and intercalary, cylindrical, (6–)11–35 μm long, proliferation
sympodial, with few to numerous conidiogenous loci, often on small
lateral shoulders, protuberant, more or less subdenticulate, short
cylindrical, 1–2(–2.5) μm diam, thickened, refractive to somewhat
darkened. Ramoconidia not observed. Conidia catenate, mostly in
branched chains, ovoid, obovoid, limoniform, ellipsoid to cylindrical,
3–20 × 2–5(–6.5) μm, 0–1(–3)-septate, septa sometimes not very
conspicuous, sometimes slightly constricted at the septa, pale
brown or olivaceous-brown, smooth or almost so to mostly minutely
verruculose, walls only slightly thickened, apex rounded, somewhat
attenuated or with a single or several conidiogenous hila, hila
protuberant, short cylindrical, truncate to slightly convex, (0.5–)
1–2(–2.5) μm diam, thickened, refractive to somewhat darkened;
microcyclic conidiogenesis not observed.
Substrate and distribution: On Chamaerops humilis; Spain.
Notes: Cladosporium chamaeropis is a biotrophic, leaf-spotting
species which is morphologically quite distinct from other taxa
described on hosts of the Arecaceae, viz., C. borassi, C. coryphae
and C. phoenicis (= C. herbarum). Cladosporium praecox,
C. maracuja and C. myrtacearum, three supericially similar
species, are easily distinguishable by deviating conidial surface
ornamentation, some additional differences in conidial size, and
conidiophore ramiication.
31. Cladosporium cheonis (Chupp & Linder) U. Braun,
Biblioth. Lichenol. 86: 85. 2003. Fig. 68.
Basionym: Cercospora cheonis Chupp & Linder, Mycologia 29: 27.
1937.
Holotype: China, Jiangsi Prov., Huang Yen Ssu, Hsing Tzu Hsien,
on leaves of Ilex sp. (Aquifoliaceae), 13 Sep. 1932, S.Y. Cheo, No.
922 (CUP 39400).
Lit.: Chupp (1954: 52), Crous & Braun (2003: 119), Schubert
(2005b: 53–54).
85
BenSch et al.
Fig. 67. Cladosporium chamaeropis (MA 06416). A. Overview. B. Conidiophores. C. Fascicle of conidiophores, conidiophores with numerous, conspicuous, somewhat darkened
conidiogenous loci. D. Conidia. Scale bars = 10 (B–D), 20 (A) µm.
margin, pale to dark. Colonies punctiform to subeffuse, dark brown
to blackish. Mycelium immersed. Stromata lacking or developed,
10–50 μm diam, substomatal, brown. Conidiophores in small to
moderately large fascicles, loose to moderately dense, arising from
internal hyphae or stromata, emerging through stomata, erect,
straight, subcylindrical to somewhat geniculate-sinuous, nonnodulose, unbranched, 40–100 × 3–5 μm, pluriseptate throughout,
pale olivaceous-brown to medium dark brown or somewhat reddish
brown, wall thin to somewhat thickened, smooth. Conidiogenous
cells integrated, terminal or intercalary, 10–30 μm long, proliferation
sympodial, with often numerous and crowded conidiogenous loci,
conspicuous, 1–1.5 μm diam, thickened and darkened. Conidia
catenate, occasionally in branched chains, ellipsoid-ovoid,
subcylindrical-fusiform, 5–23(–30) × 2–5 μm, 0–1-septate (most
conidia septate), olivaceous-brown, thin-walled, almost smooth to
verruculose, ends rounded to obconically truncate, hila 1–1.5 μm
diam, somewhat thickened and darkened.
Substrate and distribution: On Ilex sp.; China.
Fig. 68. Cladosporium cheonis (CUP 39400). Conidiophores and conidia in vivo.
Scale bar = 10 µm. U. Braun del.
Ill.: Braun (2003: 94, ig. 8), Schubert (2005b: 54, ig. 15).
In vivo: Leaf spots amphigenous, subcircular, 1–5 mm wide,
brown, blackish, later pale, greyish, surrounded by a narrow, raised
86
Notes: This species was originally assigned to Cercospora, but based
on the coronate, Cladosporium-like structure of its conidiogenous loci
and hila Braun (2003) reallocated it to Cladosporium. Cladosporium
cheonis belongs to a group of similar biotrophic, leaf-spotting species,
e.g., C. jacarandicola, C. maracuja, C. alopecuri, C. agoseridis, C.
praecox and C. orchidearum, which are, however, morphologically
well differentiated by characters of the conidiophores, conidiogenous
loci and conidia (see Schubert 2005b).
the genuS Cladosporium
32. Cladosporium chrysophylli Thaung, Trans. Brit. Mycol.
Soc. 63(3): 620. 1974. Figs 69, 70.
Holotype: Myanmar, Sintoung, east of Thazi, on living leaves of
Chrysophyllum cainito (Sapotaceae), 24 May 1973, Mya Thaung
(IMI 17741).
Lit.: Schubert (2005b: 54–56).
Ill.: Thaung (1974: 620, ig. 1), Schubert (2005b: 55, ig. 16, pl. 8,
igs A–F).
In vivo: Leaf spots amphigenous, irregular, varying in shape and
size, on the upper leaf surface pale olivaceous, greyish green,
surrounded by a narrow, dark brown margin, on the lower leaf
surface pale greenish brown, margin pale olivaceous-brown
and somewhat raised. Colonies hypophyllous, effuse, densely
caespitose, dark olivaceous-brown or somewhat greyish, short
villose or velvety. Mycelium both internal, mostly subcuticular or
substomatal, and external, supericial; hyphae branched, 2.5–6
μm wide, septate, sometimes slightly constricted at the septa,
subhyaline to pale olivaceous, smooth or sometimes distinctly
roughened, walls slightly thickened, forming swollen hyphal cells,
subglobose, 6–11 μm wide, sometimes aggregated, forming
hyphal ropes or stromatic hyphal aggregations. Conidiophores
solitary or in small loose groups or fascicles, arising from
swollen hyphal cells, internal and external hyphae or stromatic
hyphal aggregations, emerging through stomata, erumpent
through the cuticle or growing supericially, erect to sometimes
subdecumbent, straight to slightly lexuous, variable in shape
and size, somewhat dimorphic, at irst short cylindrical, 18–70 μm
long, later iliform, up to 225 μm long, often slightly geniculatesinuous, unbranched, sometimes branched, once or several
times, 3–7(–8) μm wide, septate, pale yellowish brown or pale
to medium olivaceous-brown, paler towards the apex, walls
often irregularly and distinctly roughened, rugose, outer wall
seemingly irregularly detaching, often refractive and somewhat
shiny, thickened, often distinctly two-layered, up to 2 μm wide,
mostly wider or somewhat swollen at the base, 8–12 μm wide.
Conidiogenous cells integrated, terminal and intercalary, 7–35
μm long, sub-cylindrical-oblong, sometimes slightly geniculate or
subnodulose; conidiogenous loci often situated on small lateral
shoulders, protuberant, subdenticulate, obconically truncate,
0.5–2 μm diam, convex dome often not very distinct, somewhat
thickened and darkened-refractive. Conidia catenate, mostly in
branched chains, obovoid, ellipsoid, fusiform to subcylindrical,
3–19(–28) × 2–5(–6) μm, 0–2(–5)-septate, septa not very
conspicuous, pale olivaceous-yellow to very pale brown, smooth
or almost so, with age turning irregularly rough-walled, outer wall
seemingly detached, only slightly thickened, hila more or less
protuberant, obconically truncate, 0.5–2 μm diam, convex dome
and periclinal rim often not very distinct, thickened, somewhat
darkened-refractive; microcyclic conidiogenesis occurring.
Fig. 69. Cladosporium chrysophylli (IMI 17741). Symptoms, dimorphic conidiophores
and conidia in vivo. Scale bar = 10 µm. K. Bensch del.
Substrate and distribution: On Chrysophyllum cainito; Myanmar.
Notes: Thaung (1974) described longer conidia, up to 28 μm,
which is given in brackets in the description above. Cladosporium
chrysophylli is a leaf-spotting species with unique characters,
barely comparable with other Cladosporium species.
www.studiesinmycology.org
Fig. 70. Cladosporium chrysophylli (IMI 17741). A. Symptoms. B. Conidiophore and
conidium showing coronate scar structure. C. Branched conidiophores. D. Fascicle
of conidiophores emerging through stomata. E. Tip of a conidiophore with several
somewhat darkened conidiogenous loci. F. Conidiophore, outer wall seemingly
irregularly detaching. Scale bars = 5 (B), 10 (C, E–F), 20 (D) µm.
87
BenSch et al.
33. Cladosporium chubutense K. Schub., Gresl. & Crous,
Persoonia 22: 116. 2009. Figs 71–73.
Holotype: Argentina, Chubut, dpto. Languineo, Rio Pico, Carnelia
property, 44° 8’ 30’’S, 71° 26’ 40’’W, on needles of Pinus ponderosa
(Pinaceae), Apr. 2007, A. Greslebin (CBS H-20209, dried culture).
Isotype: BAFC 51695. Ex-type cultures: CBS 124457 = CIEFAP
321 = CPC 13979.
Lit.: Bensch et al. (2010).
Ill.: Schubert et al. (2009: 116–117, igs 4–6).
In vivo: Isolated from needles becoming necrotic from the top to the
base, no discrete leaf lesions formed. Caespituli irst punctiform,
dark brown, distributed along the stomatal lines in upper and
under sides, then coalescing and forming elongated, erumpent,
black stromata. Mycelium internal, subcuticular to intraepidermal,
occasionally external, supericial, hyphae unbranched to
occasionally branched, 2–6(–8) μm wide, pluri-septate, often
in short succession, slightly constricted, often appearing to be
darkened, pale olivaceous to pale olivaceous-brown, smooth to
minutely verruculose, walls unthickened or almost so, becoming
regularly or irregularly swollen, up to 14 μm diam, walls more
thickened at swellings, sometimes forming ropes. Stromata small to
large, 20–75 μm diam or conluent, compact, dense, several layers
deep, substomatal to intra-epidermal, pseudoparenchymatous,
composed of swollen hyphal cells, subglobose to somewhat
angular, 5–10 μm diam, medium to dark brown, walls thickened.
Conidiophores macronematous, fasciculate, in small to large,
loose to dense fascicles, spider-like, emerging through stomata or
erumpent through the cuticle, arising from stromata, erect, straight
to lexuous, subcylindrical, not geniculate or only slightly so,
sometimes subnodulose due to small lateral swellings, unbranched
or rarely once branched towards the apex, 28–120 × (4–)5–8(–9)
μm, often slightly to distinctly attenuated towards the apex, base
often wider or swollen, up to 10 μm wide, pluriseptate, septa often
in short succession, sometimes slightly constricted, medium to dark
olivaceous-brown or brown, paler at apices, smooth to minutely
verruculose or irregularly and distinctly roughened, rugose, outer
walls seeming to detach irregularly, especially towards the apex,
walls thickened, often distinctly 2-layered, up to 1.5 μm wide,
sometimes enteroblastically proliferating. Conidiogenous cells
integrated, terminal and intercalary, subcylindrical, not geniculate,
sometimes subnodulose with loci situated on small lateral
shoulders, 6–32 μm long, with a single or several conspicuous
loci, somewhat crowded towards the apex, 1–2(–2.5) μm diam,
somewhat thickened and darkened-refractive. Conidia solitary or
catenate, in unbranched or branched chains, straight, subglobose,
obovoid, ellipsoid to subcylindrical, 4.5–19 × 4.5–7(–8) μm (av.
± SD: 11.1 ± 3.5 × 5.8 ± 1.0), 0–1(–2)-septate, rarely with three
septa, septum more or less median, sometimes slightly constricted,
with age becoming sinuous, pale to medium olivaceous-brown,
almost smooth to minutely verruculose, walls somewhat thickened,
attenuated towards apex and base, often broadly rounded at
the distal end, sometimes slightly pointed, with a single, two, or
rarely three hila at the distal end, conspicuous, 1–2(–2.5) μm
diam, somewhat thickened and darkened-refractive; microcyclic
conidiogenesis not observed.
In vitro: Mycelium mainly immersed, sometimes supericial; hyphae
mainly unbranched, 1–4 μm wide, septate, septa occasionally
88
Fig. 71. Cladosporium chubutense (HAL 2323 F). Fascicle of conidiophores and
conidia in vivo. Scale bar = 10 µm. K. Bensch del.
Fig. 72. Cladosporium chubutense (CBS 124457 = CPC 13979). Conidiophores and
conidia in vitro. Scale bar = 10 µm. K. Bensch del.
darkened, without any swellings and constrictions, subhyaline to pale
dingy brown or greyish brown, almost smooth to somewhat irregularly
rough-walled, walls unthickened. Conidiophores macronematous
and micronematous, arising terminally from ascending hyphae, or
sometimes laterally from plagiotropous hyphae, solitary, erect, straight
or slightly lexuous, subcylindrical to cylindrical, slightly to distinctly
geniculate-sinuous towards the apex, sometimes subnodulose
with loci situated on small lateral shoulders, often several times,
unbranched or once branched, 10–200 × (1.5–)2.5–4 μm, septate,
not constricted at septa, greyish brown to olivaceous-brown, smooth
to minutely verruculose or verruculose, especially towards the apex,
walls only slightly thickened, about 0.5 μm wide. Conidiogenous
cells integrated, terminal and intercalary, subcylindrical to cylindrical,
slightly to distinctly geniculate, once or several times, non-nodulose
but the whole apical cell occasionally inlated, ellipsoid, 10–57 μm
long, conidiogenous loci conspicuous, often crowded towards the
apex, up to six or more loci per cell, 1–2(–2.2) μm diam, somewhat
the genuS Cladosporium
Fig. 73. Cladosporium chubutense (CBS 124457 = CPC 13979). A–E. Macro- and microne-matous conidiophores with conidia. F–G. Conidial chains. Scale bars = 10 µm.
thickened and darkened-refractive. Ramoconidia subcylindrical,
19–34(–38) × (3.5–)4–5 μm, aseptate, concolorous with the tips of
conidiophores. Conidia catenate, in branched chains, branching in
all directions, up to three or four conidia in the unbranched parts,
small terminal conidia obovoid to ellipsoid, 4–8 × 2.5–4 μm (av. ± SD:
5.9 ± 1.2 × 3.0 ± 0.5), aseptate, broadly rounded at the apex, hila
0.5–1 μm diam, intercalary conidia ellipsoid-ovoid, 7–14 × 3–4.5 μm
(av. ± SD: 9.9 ± 2.0 × 3.9 ± 0.5), aseptate, with a single sometimes
up to three distal hila, hila 0.8–1.5 μm diam, secondary ramoconidia
ellipsoid-ovoid to subcylindrical, 13–27(–34) × 4–5 μm (av. ± SD:
19.8 ± 5.4 × 4.4 ± 0.4), 0–1-septate, not constricted at septa, septa
median or somewhat in the upper half, with up to four distal hila, pale
to medium greyish brown or olivaceous-brown, minutely verruculose
to usually verruculose, occasionally distinctly verrucose, walls
unthickened or only slightly so, hila conspicuous, (0.5–)0.8–2(–2.2)
μm diam, somewhat thickened and darkened-refractive; microcyclic
conidiogenesis sometimes generating conidia irregular in outline.
Culture characteristics: Colonies on PDA attaining 50–61 mm diam
after 1 mo, dull green to dark grey-olivaceous, reverse iron-grey,
velvety to powdery, margin white, broad, glabrous, aerial mycelium
pale olivaceous-grey, diffuse, loose, only few areas covered,
growth regular, lat, without conspicuous exudates, sporulation
www.studiesinmycology.org
profuse. Colonies on MEA reaching 26–37 mm diam after 1 mo,
olivaceous to greenish olivaceous forming concentric zones,
reverse iron-grey to greenish black, velvety, margin white, narrow,
regular, feathery, aerial mycelium pale olivaceous-grey, sparse,
growth lat, sometimes with a crater-like structure in the centre or
wrinkled, without conspicuous exudates, sporulation profuse.
Substrate and distribution: On Pinus ponderosa; Argentina.
Additional specimens examined: Argentina, Chubut, dpto. Languineo, Rio Pico,
Carnelia property, 44° 8’ 30’’S, 71° 26’ 40’’W, on needles of Pinus ponderosa
(Pinaceae), 12 Jan. 2005, A. Greslebin, mixed infection with C. macrocarpum and
an alternarioid hyphomycete (HAL 2323 F).
Notes: On its host, Pinus ponderosa, Cladosporium chubutense,
a species with unclear biology and ecology, is dissimilar to other
species, except for C. oreodaphnes, which has, however, longer
and somewhat narrower nodulose conidiophores (Schubert
2005b), as well as C. antarcticum distinguished by its dimorphic
mycelium, 0–3-septate conidia and its narrower conidiogenous loci
and hila, 0.8–1.5(–2) μm (Schubert et al. 2007b).
89
BenSch et al.
34. Cladosporium cladosporioides (Fresen.) G.A. de Vries,
Contr. Knowl. Genus Cladosporium: 57. 1952. Figs 74–76.
Basionym: Penicillium cladosporioides Fresen., Beitr. Mykol. 1: 22.
1850.
≡ Hormodendrum cladosporioides (Fresen.) Sacc., Michelia 2(6): 148.
1880.
≡ Cladosporium herbarum f. hormodendroides Ferraris, Flora Ital. Crypt.,
Pars I, Fungi, Fasc. 8: 332. 1912
Type: Germany, on overwintered leaves of Hydrangea sp.
(Hydrangeaceae) (not preserved). Neotype (designated in Bensch
et al. 2010): Germany, isol. from indoor air, Ch. Trautmann (CBS
H-20428). Ex-type culture: CBS 112388.
= ? Ramularia meliloti Ellis & Everh., Erythea 2: 26. 1894 [holotype: NY].
= Cladosporium myriosporum Ellis & Dearn., Proc. Canad. Inst., N.S., 3, 1: 90.
1897, p.p. (nom. confus., type material composed of C. macrocarpum and C.
cladosporioides) [syntypes: DAOM, NYS].
= Monilia humicola Oudem., Arch. Néerl. Sci. Exact. Nat. 7: 286. 1902.
= Cladosporium herbarum f. camelliae-japonicae Bubák, in RabenhorstPatzschke, Fungi Eur. Exs., Cent. 43, No. 4289. 1901, nom. nud. [syntypes:
e.g. B, HAL, HBG].
Lit.: Yamamoto (1959: 3), Ellis (1971: 319), Subramanian (1971:
285), Shvartsman et al. (1975: 93–94), Domsch et al. (1980: 202),
Ellis & Ellis (1985: 290, 468), Wang & Zabel (1990), Braun (1998:
301), Ho et al. (1999: 121), Samson et al. (2000: 108), de Hoog
et al. (2000: 583), Samson et al. (2001: 340), Zhang et al. (2003:
69–74), Park et al. (2004), Schubert & Braun (2004: 304), Heuchert
et al. (2005: 46–47), Pasqualetti et al. (2005), Schubert (2005b:
155–156), Bensch et al. (2010: 29–34).
Ill.: Fresenius (1850: Taf. 3, igs 23–28), de Vries (1952: 58–59, igs
10–11), Yamamoto (1959: 4, igs 9–12), Ellis (1971: 318, ig. 219
C), Domsch et al. (1980: 203, ig. 82), Ho et al. (1999: 122, igs
8–9), de Hoog et al. (2000: 583–584, igs), Samson et al. (2000:
108, ig. 48; 109, pl. 46), Schell (2003: 582, ig. 16), Zhang et al.
(2003: 70, ig. 30), Bensch et al. (2010: 30–32, igs 17–19).
Exs.: Rabenhorst, Fungi Eur. Exs. 4289.
In vivo: Colonies usually effuse, sometimes rather inconspicuous,
greyish green, olivaceous-brown or brownish. Mycelium usually
immersed in the substratum. Stromata lacking. Conidiophores
solitary to loosely caespitose, erect, long, iliform or subcylindrical,
neither distinctly geniculate-sinuous nor nodulose, usually
unbranched, occasionally once branched, 10–250 × (2.5–)3–5(–
6) μm, mostly less than 100 μm long, aseptate to pluriseptate
throughout, smooth, rarely faintly rough-walled, thin-walled, ≤
1(–1.5) μm. Conidiogenous cells integrated, terminal, rarely also
intercalary, occasionally conidiophores aseptate, i.e., reduced to
conidiogenous cells, 10–30(–40) μm long, usually not or barely
geniculate, i.e., usually without any proliferation, neither sympodial
nor percurrent, but often subdenticulate, with 1–3, rarely more,
denticle-like, somewhat protuberant conidiogenous loci at the
apex, (0.5)1–2(–2.5) μm diam, distinctly coronate, somewhat
darkened-refractive. Conidia in branched chains, broadly
subglobose, ellipsoid-ovoid, limoniform, (3–)4–8(–11) × 2–4(–5)
μm, 0(–1)-septate, ramoconidia and secondary ramoconidia
ellipsoid-subcylindrical to cylindrical, 8–30 × (2.5–)3–5(–5.5) μm,
0–2-septate, subhyaline, pale olivaceous to olivaceous-brown,
smooth, rarely with faintly rough-walled conidia mixed with
smooth conidia, thin-walled, ≤ 1 μm, ends attenuated to rounded,
hila (0.5)1–2(–2.5) diam, somewhat protuberant and somewhat
darkened-refractive.
90
Fig. 74. Cladosporium cladosporioides (CBS 112388). Macro- and micronematous
conidiophores, ramoconidia and conidial chains in vitro. Scale bar = 10 µm. K.
Bensch del.
In vitro: Mycelium immersed, rarely supericial; hyphae sparse,
unbranched or sparingly branched, (1−)2−4(−5) μm wide,
septate, septa occasionally darkened, without any swellings and
constrictions, subhyaline, pale olivaceous-brown or pale brown,
smooth to minutely verruculose or rough-walled, walls unthickened.
Conidiophores solitary, macronematous or semimacronematous,
sometimes micronematous, arising terminally from ascending
hyphae or laterally from plagiotropous hyphae, straight to somewhat
lexuous, narrowly cylindrical to cylindrical-oblong, sometimes
iliform, non-nodulose, usually not geniculate-sinuous, occasionally
once geniculate, 40−300(−350) × (2.5−)3−4(−5.5) μm, unbranched
or occasionally branched, branches usually short, only as peg-like
lateral outgrowth just below a septum, occasionally up to 60 μm,
mostly in the upper third, pluriseptate, usually not constricted at
septa, sometimes slightly constricted and one of the upper septa
slightly darkened where ramoconidia are formed, pale to medium
olivaceous-brown or brown, smooth to minutely verruculose or
verruculose especially towards the base, walls unthickened or
slightly thickened, occasionally slightly attenuated towards the
apex, base sometimes swollen, up to 7 μm wide; micronematous
conidiophores shorter, narrower, paler, unbranched, 9−150 ×
(1−)1.5−2.5(−3) μm wide. Conidiogenous cells integrated, usually
terminal, sometimes intercalary with conidiogenous loci situated
on small peg-like or denticle-like lateral outgrowths just below a
septum, cylindrical-oblong, not geniculate, non-nodulose, (7−)16−38
μm long, with up to four loci crowded at the apex, subdenticulate to
denticulate, protuberant, 1−2(−2.5) μm diam, central dome often
not very conspicuous, lat, somewhat thickened and darkenedrefractive. Ramoconidia seceding at one of the upper, somewhat
darkened septa, straight to slightly curved, cylindrical-oblong,
the genuS Cladosporium
Fig. 75. Cladosporium cladosporioides (CBS 112388). A–F. Macronematous conidiophores and conidial chains. Scale bar = 10 µm.
15−50 × (2.5−)3−5 μm, with up to three septa, pale olivaceousbrown, concolorous with tips of conidiophores, smooth, base not
cladosporioid, 2.5−4 μm wide, unthickened or slightly thickened,
sometimes slightly refractive. Conidia numerous, catenate, in long
branched chains, up to 10 conidia in the upper unbranched part,
branching in all directions, small terminal conidia subglobose,
obovoid, ovoid to limoniform, 3−6(−7) × (1.5−)2−2.5(−3) μm (av. ±
SD: 4.7 ± 0.9 × 2.4 ± 0.3), aseptate, intercalary conidia limoniform,
ellipsoid-ovoid, sometimes fusiform or subcylindrical, 5−12(−14.5) ×
(2−)2.5−3(−4) μm (av. ± SD: 8.1 ± 2.2 × 2.9 ±0.3), aseptate, with up
to 3(−4) distal hila, secondary ramoconidia ellipsoid, subcylindrical
to cylindrical-oblong, (7−)10−33(−38) × (2−)2.5−4(−6) μm (av. ± SD:
19.4 ± 6.6 × 3.2 ± 0.5), 0(−1)-septate, occasionally with two septa, not
constricted at septa, with up to four distal hila, subhyaline, pale brown
or pale olivaceous-brown, smooth, under SEM smooth or surface
with somewhat irregularly reticulate structure or embossed stripes
probably caused by diminishing turgor and shriveling of tender young
conidia, thin-walled, sometimes cell structure unusual, with a small
cavity in the cells, hila conspicuous, subdenticulate to denticulate,
0.5−2(−2.5) μm diam, somewhat thickened and darkened-refractive;
microcyclic conidiogenesis occasionally occurring.
Culture characteristics: Colonies on PDA grey-olivaceous to
dull green or olivaceous-grey, reverse iron-grey, leaden-grey or
olivaceous-black, velvety to loccose, margins grey-olivaceous
to white, feathery, regular, aerial mycelium sparse, diffuse, or
www.studiesinmycology.org
sometimes abundantly formed, dense, loccose-felty, low, forming
mats, growth lat to low convex, usually without prominent
exudates, occasionally with several small prominent exudates.
Colonies on MEA grey-olivaceous to olivaceous or olivaceous-grey,
pale olivaceous-grey or whitish due to aerial mycelium, olivaceousblack or olivaceous-buff at margins, reverse olivaceous-black or
iron-grey, velvety to loccose, margins white to grey-olivaceous,
glabrous to feathery, aerial mycelium sparse, scattered, diffuse to
loccose, sometimes abundantly formed, covering almost the whole
colony, loccose-felty, whitish, growth lat to effuse, somewhat
radially furrowed, without prominent exudates. Colonies on OA
grey-olivaceous, towards margins at irst greenish olivaceous,
then dull-green and again grey-olivaceous, sometimes white,
reverse olivaceous-grey to leaden-grey, sometimes pale mousegrey, velvety to loccose, margins narrow, glabrous, regular, aerial
mycelium scattered to sometimes abundant, loccose or felty,
loose to somewhat dense, growth lat, no prominent exudates;
sporulation usually profuse on all media.
Substrates and distribution: On fading and decaying plant material,
on living leaves as secondary invader, isolated from air, soil,
foodstuffs, water-damaged building materials and numerous other
materials; cosmopolitan.
Additional specimens examined: Sine loco, deposited by C.L. Shear, stored as “C.
herbarum” (CBS 132.29). Australia, New South Wales, Mullion Creek, S 33º06’48’’
E149º08’45’’, isol. from Eucalyptus robertsonii ssp. hemisphaerica (Myrtaceae),
91
BenSch et al.
Fig. 76. Cladosporium cladosporioides
(CBS 112388, neotype). A. Overview of a
colony containing running differentiated
conidiophore-forming hyphae and many
aerial hyphae. B. Scars on a conidiophore.
C. Top view of a conidiophore with scars and
an aerial structure. D. Branching patterns of
aerial hyphae intermingled with spore-forming
structures. E. Aerial hyphae, conidiophores
and spores. F. Conidiophores sprouting from
agar with all types of dispersion structures.
G. Conidial chains. H. Detail of conidial
chains and ornamentation showing irregularly
reticulate structures or embossed stripes
probably caused by diminishing turgor and
shriveling of tender young conidia. I. Three
secondary ramoconidia. J. Secondary
ramoconidia and conidia on agar with some
irregularly reticulate ornamentation. Scale
bars = 2 (B–C, H), 5 (F–G, I–J), 10 (D–E), 50
(A) µm.
15 Jan. 2007, coll. B.A. Summerell, isol. P.W. Crous (NSW 134279, CPC 13667,
13669); Queensland, near Cairns, isol. from Eucalyptus sp. (Myrtaceae), 27 Aug.
2006, P.W. Crous (CPC 13235). Brazil, isol. from soil, CBS 101367. Denmark, isol.
from cellulose powder, paint manufacturer, 2007, B. Andersen (BA 1692 = CPC
14293); isol. from soil, pea ield, 2007, B. Andersen (BA 1691 = CPC 14292).
France, Vallon, Pont d’Arc, isol. from twigs of an unidentiied tree, 21 Aug. 2007,
P.W. Crous (CPC 14271). Germany, isol. from Pisum sativum (Fabaceae), stored
as C. cladosporioides f. pisicola (CBS 145.35 = MUCL 926); Essen, botanical
garden, 51.45, 7.0167, isol. from leaves of Morus rubra (Moraceae), 2005, N. AleAgha (CPC 12214). India, isol. from Dalbergia sp. (Fabaceae), 3 Jan. 2004, coll. W.
Gams, isol. P.W. Crous (CPC 11131); isol. from Eucalyptus sp., 3 Jan. 2004, coll.
W. Gams, isol. P.W. Crous (CPC 11161). Indonesia, Tele, isol. from Eucalyptus sp.,
endophyte spots, spots after herbicide, 3 Jan. 2008, coll. M.J. Wingield, isol. P.W.
Crous, as “Neofusicoccum sp.” (CPC 15038). Israel, Jaffa, isol. from Gossypium
seeds (Malvaceae), 1967, isol. by M. Gonen (CBS 674.82 = CBS 320.87 = ATCC
38026 = ATCC 200936 = IMI 126640, stored as “C. tenuissimum”). Japan, isol.
from bamboo slats, probably authentic strain of C. multigeniculatum (CBS 122130
= ATCC 38012 = IFO 6539 = JCM 10684 = NBRC 6539). Slovenia, Češnjica
near Ljubljana, isol. from a living mite inhabiting a strawberry leaf, 4 Apr. 2008,
Vojko Škerlavaj, isol. by H.-J. Schroers (HJS 1069 = CPC 15167). South Africa,
isol. from Pisum sativum, B.J. Dippenaar, stored as C. cladosporioides f. pisicola
(CBS 143.35 = MUCL 10090); Barberton, Laeveld Coop, isol. from wheat, 1988
(CPC 14009 = MRC 10150); Eastern Cape, Aiwal North, isol. from wheat, 1989
(CPC 14019 = MRC 10813); Free State, Brandfort, isol. from wheat, 1989 (CPC
14017 = MRC 10809, CPC 14018 = MRC 10810); Perdespan, isol. from wheat,
92
1988 (CPC 14015 = MRC 10260); Winburg, isol. from wheat, 1989 (CPC 14021
= MRC 10827); Gauteng, Pretoria, isol. from pawpaw, 1990 (CPC 14024 = MRC
11280). South Korea, Chuncheon, N37º50’10’’ E127º32’01’’, isol. from Celosia
cristata (Amaranthaceae), 7 Oct. 2003, coll. H.-D. Shin, isol. P.W. Crous (CPC
11121); Suwon, N37º16’03’’ E126º59’16’’, isol. from chasmothecia of Phyllactinia
sp. (Erysiphales) on Fraxinus chinensis subsp. rhynchophylla (Oleaceae), 7 Nov.
2007, coll. H.-D. Shin, isol. P.W. Crous (CPC 14705); Chuncheon, N37º50’10’’
E127º32’01’’, isol. from Phragmidium griseum on Rubus crataegifolius (Rosaceae),
20 Jul. 2004, coll. H.-D. Shin, isol. P.W. Crous (CPC 11398); Jinju, N35º11’24’’
E128º10’56’’, isol. from Phytolacca americana (Phytolaccaceae), 15 Oct. 2003,
coll. H.-D. Shin, isol. P.W. Crous (CPC 11122); Jinju, N35º11’24’’ E128º10’56’’,
isol. from Plectranthus sp. (Lamiaceae), 1 Jul. 2004, coll. H.-D. Shin, isol. P.W.
Crous (CPC 11406); Chuncheon, N37º50’10’’ E127º32’01’’, isol. from Ricinus
communis (Euphorbiaceae), 7 Oct. 2003, coll. H.-D. Shin, isol. P.W. Crous (CPC
11119); Yangpyeong, N37º30’12’’ E127º41’55’’, isol. from Rubus coreanus, 23 Jul.
2004, coll. H.-D. Shin, isol. P.W. Crous (CPC 11404); Hongcheon, N37º48’17’’
E127º51’13’’, isol. from leaves of Stellaria aquatica (Caryophyllaceae), 6 Jun.
2005, coll. H.-D. Shin, isol. P.W. Crous (CPC 12187); Hoengseong, N37º32’09’’
E128º07’07’’, isol. from Valeriana oficinalis (incl. V. fauriei) (Valerianaceae), 23
Jun. 2004, coll. H.-D. Shin, isol. P.W. Crous (CPC 11393); Jeongeup, N35º36’05’’
E126º51’25’’, isol. from Vigna unguiculata [= V. sinensis] (Fabaceae), 29 Oct. 2003,
coll. H.-D. Shin, isol. P.W. Crous (CPC 11123); Suwon, N37º16’03’’ E126º59’16’’,
isol. from Viola mandshurica (Violaceae), 14 Oct. 2003, coll. H.-D. Shin, isol. P.W.
Crous (CPC 11120); Hongcheon, N37º48’17’’ E127º51’13’’, isol. from Chenopodium
icifolium (Chenopodiaceae), 10 Mar. 2002, coll. H.-D. Shin, isol. P.W. Crous (CPC
the genuS Cladosporium
10142). Thailand, Chiang Mai, Mushroom Research Centre, isol. from Areca sp.
(Arecaceae), 20 Dec. 2006, coll. I. Hidayat, isol. P.W. Crous (CPC 13734). Uganda,
Mubende, isol. from food, coffee leaf, 2000, coll. J.L. Sørensen, isol. B. Andersen
(BA 1677 = CPC 14356). USA, 2004, M. Blackwell (CPC 11684 = CBS 117483,
as “C. gossypiicola”); California, isol. from Pisum sativum, as C. cladosporioides
f. pisicola (CBS 144.35 = ATCC 11284 = IFO 6371 = IMI 049627); Laramie,
isol. from food, mouldy pea, 2000, coll. J.L. Sørensen, isol. B. Andersen (BA 1676
= CPC 14355); Louisiana, Baton Rouge, isol. from Magnolia sp. (Magnoliaceae), 8
Sep. 2007, P.W. Crous (CPC 14244); isol. from pruned wood, 2006, K. Seifert (CPC
12852); Washington, isol. from spinach seed, Spinacia oleracea (Chenopodiaceae),
2003, L. du Toit (CBS 126341 = CPC 12763, CPC 12760, 12762, 12764); isol. from
grape berry, F.M. Dugan (w99-175a sci 1 = CBS 113740); isol. from grape bud, F.M.
Dugan (113db sci 1 = CBS 113738); isol. from culm node of crested wheat grass,
F.M. Dugan (wa2-00 sci 1 = CBS 113739).
Excluded strains within the C. cladosporioides complex [morphologically
indistinguishable but phylogenetically distinct, indicated in Bensch et al. (2010), Fig.
1 as C. cladosporioides s. lat. Lineages 1−4]: Argentina, Chubut, Rio Pico, carnelian
property, isol. from needles of Pinus ponderosa (Pinaceae), 2007, A. Greslebin (CPC
13978). Denmark, isol. from indoor building material, school, 2007, B. Andersen
(BA 1695 = CPC 14296). Germany, isol. from leaves of Acer pseudoplatanus
(Aceraceae), L. Pehl, ident. as C. tenuissimum by G.S. de Hoog (CBS 116744);
isol. from wheat grain, Triticum sp. (Poaceae), 2007, B. Andersen (BA 1674 = CPC
14284); Bayern, isol. from lichens on leaves of Acer platanoides (Aceraceae), 2006,
B. Heuchert (CPC 13220); Tübingen, botanical garden, isol. from Paeonia obovata
(Paeoniaceae), Sep. 2006, P.W. Crous (CPC 13362). Netherlands, isol. from seed
coat of Cirsium vulgare (Asteraceae), depos. by B.H. van Leeuwen, Jan. 1980
(CBS 125.80); Millingerwaard, isol. from fruits of Sambucus nigra (Adoxaceae), 29
Aug. 2007, P.W. Crous (CPC 14238). New Zealand, Auckland, Auckland University
campus, Oncoba spinosa (Salicaceae), 9 Jan. 2004, C.F. Hill (Hill 1076-2 = CPC
11664); Waikato, Karapiro, Gorton Road, isol. from imported buds of Prunus avium
(Rosaceae), 6 Jan. 2008, J. Rennie (CPC 15457). South Africa, Western Cape
Province, Jonkershoek Nature Reserve, isol. from Brunneosphaerella protearum
(Mycosphaerellaceae) fruiting bodies, 30 Mar. 2007, P.W. Crous (CPC 13867).
South Korea, Pocheon, National Arboretum, N37º45’04’’ E127º09’55’’, isol. from
Fatoua villosa (Moraceae), 18 Oct. 2002, coll. H.-D. Shin, isol. P.W. Crous (CPC
10150). UK, Manchester, isol. from uredospores of Puccinia allii (Pucciniaceae),
G.S. Taylor (CBS 306.84). USA, Washington State, isol. from bing cherry fruits, R.G.
Roberts (CBS 113746).
Notes: Cladosporium cladosporioides as previously circumscribed
on the base of morphology represents a heterogeneous complex of
numerous phylogenetically and more or less also morphologically
distinct species (Bensch et al. 2010). Cladosporium cladosporioides
s. str. is one of the most common, saprobic Cladosporium species
with worldwide distribution, frequently occurring as secondary invader
on necrotic parts of many different host plants, isolated from air, soil,
textiles and numerous other substrates (Ellis 1971) and found as
common endophytic fungus (Riesen & Sieber 1985, El-Morsy 2000,
Kumaresan & Suryanarayanan 2002). Furthermore, the conidia of
this species are among the most ubiquitous bioaerosols found in
indoor and outdoor samples (Domsch et al. 1980, Mullins 2001,
Park et al. 2004). Some leaf-spotting races have been reported, but
such host-speciic pathogenicity is largely untested (Anilkumar &
Seshadri 1975, Arya & Arya 2003). Bensch et al. (2010) proposed a
neotype for C. cladosporioides since type material of this species is
not preserved. De Vries (1952) invalidly and erroneously lectotypiied
the species based on Bisby’s dried “standard culture” [isol. fr. Arundo
leaves, Bamboo Garden, Kew, 1943 (IMI 25324, 60507, 60509)
= CBS 170.54] which, however, proved to belong to the saprobic
C. ramotenellum, which is undoubtedly more common than originally
presumed by Schubert et al. (2007b).
In phylogenetic sequence analyses, this species forms a
distinct, well-supported clade (see Bensch et al. 2010, ig 1, part c).
Nevertheless, C. cladosporioides is still paraphyletic representing a
species complex since there are still numerous isolates listed under
excluded strains that are morphologically indistinguishable from C.
cladosporioides s. str., but phylogenetically different, clustering
apart in various subclades.
www.studiesinmycology.org
Yamamoto (1959), Ellis (1971), de Hoog et al. (2000) and
Samson et al. (2000) discussed strains of “C. cladosporioides” with
asperulate or inely verruculose conidia, which proved to represent
different, phylogenetically clearly distinct species, as for instance
C. asperulatum and C. perangustum.
Cladosporium cladosporioides, which occurs rather commonly
on Pisum sativum, has often been confused with C. cladosporioides
f. pisicola (≡ C. pisicola). The latter species is, however, a
biologically and morphologically distinct species (see notes under
C. pisicola).
35. Cladosporium colocasiae Sawada, Trans. Nat. Hist.
Soc. Taiwan 25: 125. 1916. Figs 77, 78.
Holotype: Taiwan, on Colocasia esculenta (= C. antiquorum)
(Araceae), 2 Jun. 1910, K. Sawada (PPMH). Ex-type culture: CBS
386.64 = ATCC 200944 = MUCL 10084.
= Cladosporium colocasiicola Sawada, Special Publ. Coll. Agric. Natl. Taiwan
Univ. 8: 195. 1959, nom. inval.
Lit.: Bugnicourt (1958), Ellis (1971: 312), Matsushima (1975: 34),
David (1988a), IMI Distribution Maps of Plant Diseases 592 (1988),
Holcomb (1989), Ho et al. (1999: 123), Zhang et al. (2003: 74–76),
Schubert (2005b: 56–58), Bensch et al. (2010: 34–35).
Ill.: Bugnicourt (1958: 235, ig. 1), Ellis (1971: 313, ig. 216 B),
Matsushima (1975: pl. 77), David (1988a: ig.), De & Chattopadhyay
(1994: 228, ig. 4), Ho et al. (1999: 124, igs 10–11), Zhang et al.
(2003: 76, ig. 40), Schubert (2005b: 57, ig. 17, pl. 9, igs A–E),
Bensch et al. (2010: 35, fig. 20).
In vivo: Leaf spots amphigenous, subglobose, oval to somewhat
irregular, at irst punctiform, later extending, forming large patches,
very variable in size, 1–10(–15) mm wide, sometimes conluent,
covering large areas of the leaf surface, medium to dark brown,
Fig. 77. Cladosporium colocasiae (BPI 426382). Conidiophores and conidia in vivo.
Scale bar = 10 µm. K. Bensch del.
93
BenSch et al.
Fig. 78. Cladosporium colocasiae in vivo (BPI 426382; J–N) and in vitro (CBS 386.64 and 119542; A–I). A–D. Conidiophores and conidial chains. E–F. Intercalary conidiogenous
cells with typical nodes and conidiogenous loci restricted to these swellings. G–H. Microcyclic conidiogenesis with conidia forming secondary conidiophores. I. Conidia. J.
Nodose conidiophore showing coronate scar structure. K. Conidiophore with distinct swellings, clearly separated and distant from each other. L. Conidiophore with coronate
scars conined to swellings. M. Conidiophore. N. Conidia. Scale bars = 5 (J), 10 (A–I, L–N), 20 (K) µm.
later yellow, most frequently on old leaves, giving them a spotted
appearance, occasionally somewhat paler in the centre, on
the lower leaf surface somewhat paler, grey-brown. Colonies
amphigenous, effuse, mostly densely caespitose, brown, velvety to
villose. Mycelium internal, subcuticular to intraepidermal, later also
external, growing supericially; hyphae branched, 2.5–7.5 μm wide,
septate, often with swellings and constrictions, at swellings up to 10
μm wide, pale brown to somewhat yellowish brown, smooth, thickwalled, forming hyphal aggregations, composed of swollen hyphal
cells, subglobose to somewhat angular, 4–10 μm wide, pale to
pale medium brown or somewhat yellowish brown, smooth, thickwalled. Conidiophores solitary or in small loose groups, arising
from internal and external hyphae or from hyphal aggregations,
conidiophores arising from internal hyphae, erumpent through the
cuticle, erect, straight or slightly lexuous, somewhat geniculate94
sinuous, subnodulose to distinctly nodulose or even nodose,
usually with multilateral, intercalar and terminal swellings,
5–8.5(–10) μm diam, unbranched, sometimes once branched,
(25–)50–250(–450) μm long or even longer, 3.5–6(–7) μm wide,
pluriseptate, not constricted at the septa, pale to pale medium
brown, smooth, with age somewhat asperulate, walls thickened,
usually one-layered, sometimes also two-layered, especially
near the base, somewhat swollen at the base and attenuated
towards the apex. Conidiogenous cells integrated, terminal and
intercalary, 6–30 μm long, with characteristic nodulose swellings,
conidiogenous loci conined to them, situated on small shoulders
or multilateral swellings, mostly 1–3 per node, protuberant, almost
lat to short cylindrical, well differentiated in dome and raised rim,
1–2(–2.5) μm diam, thickened, somewhat refractive or slightly
darkened. Conidia catenate, in unbranched or branched chains,
the genuS Cladosporium
broadly ellipsoid-subcylindrical to cylindrical, (8–)12–23(–32) ×
(5–)6–9(–10) μm, 0–3(–5)-septate, sometimes slightly constricted
at the median septum, pale to pale medium brown, smooth, surface
of older conidia sometimes appearing to be net-like, granular or
punctate, walls more or less thickened, ends broadly rounded, hila
protuberant, 1–2(–2.5) diam, thickened, refractive to somewhat
darkened; sporadically microcyclic conidiogenesis occurring.
In vitro: Mycelium immersed and supericial; hyphae loosely
branched, 1–4 μm wide, sometimes distinctly swollen, bulboid,
about 10 μm wide, septate, not constricted at septa, subhyaline
to pale olivaceous-brown, smooth to loosely verruculose, walls
unthickened. Conidiophores macronematous, solitary, arising
terminally and laterally from hyphae, erect, lexuous, cylindricaloblong, nodose, with several nodes being quite apart from each other,
occasionally branched, very long, up to 1 350 μm or even longer,
3–4(–5) μm wide, nodes multilateral, 5–8 μm diam, pluriseptate, not
constricted at septa, pale to medium olivaceous-brown, smooth or
sometimes appearing to be reticulate, walls somewhat thickened,
about 0.5 μm wide. Conidiogenous cells integrated, terminal and
intercalary, cylindrical-oblong, nodose with a single node per cell,
15–70 μm long, loci restricted to swellings, usually 2–4 per node,
1–1.8(–2) μm diam Ramoconidia occasionally formed. Conidia
solitary or in short, unbranched or branched chains, more or less
straight, broadly ellipsoid-subcylindrical to cylindrical, unbranched
terminal conidia 9–16 × 5–7(–8) μm (av. ± SD: 12.5 ± 1.8 × 6.1
± 0.7), 0–1-septate, catenate conidia 10.5–23(–30) × 5–8(–9) μm
(av. ± SD: 17.5 ± 5.2 × 6.0 ± 1.0), 0–1(–2)-septate, septum median
or somewhat in the upper or lower half or third, becoming sinuous
with age, pale to medium brown, smooth to loosely verruculose
or reticulate, walls unthickened or almost so, hila conspicuous,
1–1.8(–2) μm diam, somewhat thickened and darkened-refractive;
microcyclic conidiogenesis sometimes occurring with conidia
forming secondary conidiophores.
Culture characteristics: Colonies on PDA attaining 56–76 mm diam
after 14 d, grey-olivaceous to olivaceous or dull green, reverse
olivaceous-black, velvety, pulvinate to loccose, with a narrow white
or grey-olivaceous margin, regular to slightly undulate, somewhat
feathery, aerial mycelium sparse, locally constricted to few areas,
loccose, growth regular, lat to low convex, numerous small but not
very conspicuous exudates formed, sporulation profuse. Colonies
on MEA reaching 50–68 mm diam after 14 d, pale olivaceousgrey, grey-olivaceous to greenish olivaceous due to abundant
sporulation, whitish to smoke-grey due to aerial mycelium, reverse
olivaceous-grey to iron-grey, velvety, powdery to loccose or luffy,
with a white regular, glabrous or feathery, narrow margin, aerial
mycelium abundantly formed, loccose to luffy, covering large part
of the colony surface, growth effuse, conical, radially furrowed
and wrinkled with elevated colony centre, few small conspicuous
exudates start to be formed, sporulating. Colonies on OA, 60–65
mm diam after 14 d, grey-olivaceous, whitish or pale olivaceousgrey due to loccose aerial mycelium arranged in tufts, spotted,
reverse leaden-grey to iron-grey, pulvinate to loccose, margins
grey-olivaceous, glabrous, regular, growth lat, without prominent
exudates, sporulation profuse.
Substrate and distribution: On Colocasia esculenta (Araceae);
Africa (Ethiopia, Ghana, Guinea, Mauritius, Nigeria), Asia (Brunei,
China, Hong Kong, India, Indonesia, Japan, Malaysia, Nepal,
Pakistan, Sabah, Sarawak, South Korea, Taiwan), Australasia
(American Samoa, Australia, Cook Islands, Federated States
www.studiesinmycology.org
of Micronesia, Fiji, French Polynesia, Kiribati, Marshall Islands,
New Caledonia, New Zealand, Niue, Palau, Papua New Guinea,
Solomon Islands, Tahiti, Tonga, Vanuatu, Western Samoa), Europe
(Portugal, Azores), North America (USA), West Indies (Barbados),
Central & South America (Brazil, Cuba, Dominican Republic,
Puerto Rico).
Additional specimens examined: Dominican Republic, intercepted at San Juan,
on Colocasia esculenta (Araceae), 9 Jul. 1985, R. Barbosa (BPI 525147, as C.
colocasiicola). Ethiopia, Kaffa Prov., Jimma, on Colocasia esculenta, 10 Nov. 1955,
R.B. Stewart (BPI 426383, BPI 426385, NY). Fiji, isol. from Colocasia esculenta,
June 2001, C.F. Hill (CBS 115191 = CPC 4323 = STE-U 4323). Japan, Kyoto, on
Colocasia esculenta, 2 Oct. 1924, K. Togashi (BPI 426382); Riken, BioResource
Centre, isol. from Colocasia esculenta (CBS 119542 = CPC 12726 = ICM 13264);
Sendai, 25 Oct. 1918, A. Yasuda (BPI 426381). Puerto Rico, intercepted at San
Juan, on Colocasia esculenta, 11 Mar. 1961, H.L. Rubin (BPI 426384).
Notes: Cladosporium colocasiae is a leaf-spotting species, conined
to Colocasia esculenta, with nodulose conidiophores that are
reminiscent of those of C. herbarum, C. oxysporum and C. variabile,
but its conidia are quite distinct (Schubert 2005b, Schubert et al.
2007b). In phylogenetic trees (TEF and ACT), sequences of this
species are nested within C. tenuissimum, but form a distinct, wellsupported clade, suggesting that additional markers are necessary
for a suficient resolution. Type material of C. colocasiicola could
not be traced, but based on the original description and illustration
this name has to be reduced to synonymy with C. colocasiae.
Ho et al. (1999) examined C. colocasiae in culture and
published a irst detailed description of its features in vitro,
recording the conidiophores as being much longer than on the
natural substratum. Matsushima (1980) mentioned Eucalyptus
sp. and Psidium guajava as additional hosts, but these records
probably refer to one of the supericially similar, saprobic species
mentioned above. Zhang et al. (2003) treat C. colocasiicola as a
separate species and cite a record on Nelumbo nucifera, which is,
however, very doubtful.
36. Cladosporium colombiae K. Schub. & Crous, Persoonia
22: 120. 2009. Figs 79, 80.
Holotype: Colombia, Páramo de San Cayetano, isol. from a dead
leaf of Cortaderia (Poaceae), depos. May 1980, isol. W. Gams
(CBS H-10374), formerly stored as C. tenuissimum. Ex-type
culture: CBS 274.80 B.
Lit.: Bensch et al. (2010).
Ill.: Schubert et al. (2009: 120–121, igs 10–11).
In vitro: Mycelium immersed and supericial; hyphae branched,
1–5 μm wide, septate, sometimes constricted at septa and with
swellings, up to 8 μm diam, subhyaline to olivaceous or olivaceousbrown, smooth to minutely verruculose or irregularly rough-walled,
sometimes covered by polysaccharide-like material, wart-like,
rugose, therefore irregular in outline, often forming ropes or
loose aggregations. Conidiophores macro- and micronematous,
arising terminally or laterally from ascending or plagiotropous
hyphae, erect, straight to lexuous, solitary or in pairs of two;
macronematous conidiophores cylindrical-oblong, non-nodulose,
unbranched or occasionally branched, 25–105 × 3–4(–4.5) μm,
often slightly attenuated towards the apex, 0–4(–5)-septate,
not constricted at septa, sometimes in short succession, pale to
medium olivaceous-brown, sometimes even dark olivaceous95
BenSch et al.
Fig. 79. Cladosporium colombiae (CBS 274.80 B). Conidiophores and conidia in
vitro. Scale bar = 10 µm. K. Bensch del.
brown, smooth to often minutely verruculose, especially towards
the apex, walls slightly thickened; micronematous conidiophores
iliform, narrower, paler, often only as peg-like lateral outgrowth
of hyphae, unbranched, 10–135 × 2–2.5 μm, septate, subhyaline
to pale olivaceous-brown, smooth to minutely verruculose, walls
unthickened. Conidiogenous cells integrated, terminal, rarely
intercalary, cylindrical-oblong or iliform, 10 – 37 μm long, usually
with only a single apical locus, sometimes with 2–3 loci, then
subdenticulate, 1–2 μm diam, thickened and darkened-refractive.
Conidia catenate, in long branched chains, up to 10 conidia in the
unbranched part, small terminal conidia obovoid, 4–6.5 × 2.5–3(–4)
μm (av. ± SD: 4.7 ± 0.8 × 3.2 ± 0.5), intercalary conidia ovoid,
limoniform to ellipsoid-ovoid, 6–9 × (2.5–)3–3.5 μm (av. ± SD: 7.0
± 0.8 × 3.2 ± 0.3), aseptate, attenuated towards apex and base,
secondary ramoconidia ellipsoid to subcylindrical, sometimes
clavate, 8–17(–23) × (2.5–)3–4 μm (av. ± SD: 13.8 ± 3.4 × 3.5 ±
0.4), 0–1(–2)-septate, not constricted at the median septum, pale to
medium olivaceous-brown, smooth to minutely verruculose or often
irregularly rough-walled, walls somewhat thickened, about 0.5 μm
thick, attenuated towards apex and base, with 2–4(–5) distal hila,
subdenticulate, 0.8–2 μm diam, thickened and darkened-refractive;
microcyclic conidiogenesis sometimes occurring.
Fig. 80. Cladosporium colombiae (CBS 274.80 B). A–D. Conidiophores and conidia. E. Conidial chain. Scale bar = 10 µm.
96
the genuS Cladosporium
Culture characteristics: Colonies on PDA grey-olivaceous, reverse
iron-grey to greenish blue, luffy to felty, margin feathery, aerial
mycelium high, luffy, growth low convex to convex, without
prominent exudates, sporulation profuse. Colonies on MEA smokegrey to grey-olivaceous and pale olivaceous-grey with small dots
of olivaceous-grey towards margins, whitish or glaucous-grey
towards margins, reverse iron-grey, velvety to woolly-felty, margins
colourless to white, somewhat feathery, growth lat, exudates few
and small but conspicuous, sporulation profuse.
Substrate and distribution: On leaves of Cortaderia; Colombia.
Notes: Cladosporium colombiae is phylogenetically closely allied
to C. chubutense, but the latter species is morphologically quite
distinct by having usually distinctly geniculate, longer conidiophores
and minutely verruculose to verruculose, larger conidia formed in
short chains. Cladosporium ramotenellum, C. subtilissimum and C.
tenuissimum are additional comparable species but can be easily
distinguished by the size and shape of their conidia (Schubert et al.
2007b). The ecology of C. colombiae remains unclear with respect
to its possible role as a saprobe or pathogen.
37. Cladosporium corrugatum McAlpine, Fungus Dis.
Citrus Trees Austral.: 88. 1899. Fig. 81.
Holotype: Australia, Armadale near Melbourne, on both surfaces
of green orange leaves (Citrus ×aurantium, Rutaceae), Jan. 1899
(VPRI 5924).
Lit.: Saccardo (1913a: 1367), Schubert (2005b: 58–60).
Ill.: McAlpine (1899: ig. 57), Schubert (2005b: 59, ig. 18).
In vivo: Leaf spots amphigenous, oval to irregular, 3–18 mm
wide, pale to dark grey-brown, on the upper leaf surface with an
irregular, narrow, dark reddish brown, somewhat raised margin,
on the lower leaf surface margin wider, at irst yellowish brown,
later somewhat reddish, irregularly lobed in outline, surface of the
spots more or less corrugated. Colonies amphigenous, punctiform
to effuse, caespitose, forming dense tufts, dark brown to even
blackish, conluent, velvety. Mycelium immersed, subcuticular
to intraepidermal; hyphae sparingly branched, 3–4 μm wide,
sometimes slightly swollen and up to 6 μm wide, septate, not
constricted or slightly constricted at the septa, subhyaline to pale
olivaceous, smooth, walls not or barely thickened. Stromata mostly
substomatal, composed of subglobose to somewhat angular, loosely
to densely arranged, thick-walled cells, 5–9 μm wide, pale to dark
olivaceous-brown, smooth. Conidiophores solitary or fasciculate,
loose to dense, arising from stromata, emerging through stomata or
erumpent through the cuticle, straight to often somewhat lexuous,
somewhat geniculate-sinuous, unbranched, rarely branched, 15–
70 × 3–6 μm, septate, medium to dark olivaceous, occasionally
somewhat paler towards the apex, smooth, walls not or only slightly
thickened, often somewhat swollen at the base. Conidiogenous
cells integrated, terminal or intercalary, cylindrical, 5–25 μm long,
proliferation sympodial, with a single to several conidiogenous
loci, protuberant, subdenticulate, mostly truncate, 0.5–1.5(–2) μm
diam, periclinal rim often not very conspicuous, slightly thickened,
only somewhat darkened-refractive. Conidia catenate, in branched
chains, more or less straight, obovoid, ellipsoid to sometimes
Fig. 81. Cladosporium corrugatum (VPRI 5924). Conidiophores and conidia in vivo. Scale bar = 10 µm. K. Bensch del.
www.studiesinmycology.org
97
BenSch et al.
subcylindrical, 3–20 × 2.5–7 μm, 0–2(–3)-septate, not constricted
at the septa, pale olivaceous to olivaceous-brown, smooth, rarely
minutely verruculose, walls not to slightly thickened, apex rounded
or somewhat attenuated, with a single or few apical hila, hila
truncate to slightly convex, 0.5–1.5(–2) μm diam, slightly thickened,
somewhat darkened-refractive; microcyclic conidiogenesis
occurring.
Substrate and distribution: On Citrus ×aurantium; Australia.
Notes: Cladosporium corrugatum is a leaf-spotting species welldistinguished from other Cladosporium spp. occurring on Citrus
and other hosts. Type material of C. citri Massee, C. farnetianum
(≡ C. citri Briosi & Farneti, nom. illeg.), C. furfuraceum, and
C. subfusoideum, additional species described from Citrus, is
not preserved. Cladosporium brunneoatrum, causing “False
Melanose” on leaves and fruits of Citrus ×aurantium, is excluded
from Cladosporium s. str., but its taxonomic status remains
unknown since the type material is very sparse. Type details of
C. subfusoideum recorded on fruits of Citrus medica agree with
Diplodia citricola. Cladosporium elegans var. singaporense is
conspeciic with Spiropes guareicola (Schubert & Braun 2005a).
Cladosporium sclerotiophilum, introduced by Sawada (1931) and
only described in Japanese, has to be excluded from Cladosporium
s. str., but its taxonomic afinity is not yet clear.
38. Cladosporium coryphae (Syd. & P. Syd.) J.C. David,
Mycol. Pap. 172: 99. 1997. Fig. 82.
Basionym: Heterosporium coryphae Syd. & P. Syd., Philipp. J. Sci.
8: 196. 1913.
Lectotype (selected by David, 1997): Philippines, Mindoro, San
José, on leaves of Corypha elata (Arecaceae), Jan. 1912, P.W.
Graff, Sydow, Fungi Exot. Exs. 48 (S). Isolectotypes: IMI 10041,
K, M-0057517.
Ill.: David (1997: 89, ig. 22 I–K; 101, ig. 27).
Exs.: Sydow, Fungi Exot. Exs. 48.
In vivo: On leaves without conspicuous spots or discolorations.
Colonies amphigenous, caespitose, loose to dense, effuse,
medium olivaceous-brown to brown, velvety, covering more
or less the whole leaf surface. Mycelium external, supericial,
radiating around caespitulae, hyphae branched, 3–7 μm wide,
pluriseptate, translucent, pale to medium yellowish brown to
olivaceous-brown, smooth, walls slightly thickened, cells becoming
often somewhat swollen, subglobose, up to 15 μm wide, forming
stromatic hyphal aggregations. Conidiophores solitary or loosely
fasciculate, arsising from stromatic hyphal aggregations or from
hyphae, spreading out irregularly to give a somewhat loccose
appearance, erect, lexuous and somewhat sinuous, slightly
geniculate near the apex, unbranched, occasionally branched,
(35–)70–300(–400) × 4–7(–8) μm, slightly attenuated towards the
apex, septate, septa often not very conspicuous, pale to medium
chestnut-brown, paler brown or somewhat greyish brown near the
apex, smooth, walls somewhat thickened, sometimes two distinct
wall layers visible. Conidiogenous cells integrated, terminal to
more rarely intercalary, cylindrical-oblong to somewhat geniculate,
17–85 μm long, proliferation sympodial, with a single or few,
more or less protuberant conidiogenous loci, 1.5–3 μm diam,
98
Fig. 82. Cladosporium coryphae (S). Loosely fasciculate conidiophores and conidia
in vivo. Scale bar = 10 µm. K. Bensch del.
thickened, darkened-refractive. Conidia catenate, in unbranched
or branched chains, usually straight, occasionally slightly curved,
obovate, obpyriform, broadly ellipsoid to subcylindrical, 9–32(–44)
× (5–)7–14(–17) μm, 0–4(–5)-septate, occasionally distoseptate,
not constricted at septa, straw-coloured, becoming more densely
pigmented, ochre brown to red-brown, smooth, walls thickened,
usually 2–3-layered, appearing zonate, different zones with
different pigmentation, cells appearing to have a distinct lumen,
hila not very conspicuous, 1–3 μm diam, thickened, darkenedrefractive; microcyclic conidiogenesis occuring.
Substrate and distribution: On Corypha elata; Philippines.
Notes: This species was placed in the separate subgenus
Bistratosporium (David 1997), which is, however not tenable.
39. Cladosporium cucumerinum Ellis & Arthur, Bull. Agric.
Exp. Sta., Indiana 19: 9–10. 1889. Figs 83, 84.
Holotype: USA, New York, Geneva, on fruits of Cucumis sativus
(Cucurbitaceae), J.C. Arthur (NY).
the genuS Cladosporium
Fig. 83. Cladosporium cucumerinum (BPI 426422). Conidiophores and conidia in
vivo. Scale bar = 10 µm. K. Bensch del.
Epitype (designated in Bensch et al. 2010): isol. from fruits of C.
sativus (CBS H-20429). Ex-type culture: CBS 171.52 = MUCL
10092.
= Scolecotrichum melophthorum Prill. & Delacr., Bull. Soc. Mycol. France 7(1):
219. 1891.
≡ Macrosporium melophthorum (Prill. & Delacr.) Rostr., Gartn.-Tidende
24: 18. 1893.
= Cladosporium cucumeris A.B. Frank, Z. Planzenkrankh. 3: 31. 1893
[holotype: B 700006228].
= Cladosporium scabies Cooke, Gard. Chron., Ser. 3, 34: 100. 1903 [type: K].
= Cladosporium cucumerinum var. europaeum Bubák, in herb. [BPI 426422].
Lit.: Saccardo (1892: 601), Lindau (1907: 830, 1910: 797), Ferraris
(1912: 349), Gonzáles-Fragoso (1927: 206), Cash (1952: 68),
de Vries (1952: 62), Ellis (1971: 318), Ellis & Holliday (1972),
Brandenburger (1985: 403), Ellis & Ellis (1985: 339), von Arx (1987:
193), McKemy & Morgan-Jones (1992), Ho et al. (1999: 125),
Zhang et al. (2003: 80–82), Schubert (2005b: 60–63), Bensch et
al. (2010: 35–37).
Ill.: de Vries (1952: 63, ig. 12), Ellis (1971: 318, ig. 219 B), Ellis
& Holliday (1972: ig.), von Arx (1987: 194, ig. 83b), McKemy &
Morgan-Jones (1992: 165, ig. 1; 167, pl. 1), Ho et al. (1999: 124,
ig. 14), Zhang et al. (2003: 81, ig. 45), Schubert (2005b: 62, ig.
19), Bensch et al. (2010: 36, ig. 21).
Exs.: Petrak, Fl. Bohem. Morav. Exs. Pilze 2108; Poelt & Scheuer,
Reliqu. Petrak. 2342; Săvulescu, Herb. Mycol. Roman. 643.
In vivo: On living leaves, petioles, stems, fruits and young shoots,
causing cucumber gummosis or scab and crown blight, on
leaves forming necrotic, water-soaked spots, sometimes with a
gummy exudate, sporulation mostly only sparse; on fruits forming
pronounced, deeply sunken brownish lesions, up to 10 mm diam or
conluent, with a gummy exudate, older fruits with brown, cork-like
scabs; young seedlings sometimes seriously damaged, dieback
of apical shoots may occur. Colonies effuse, loosely to densely
www.studiesinmycology.org
caespitose, pale to medium or almost dark greyish olive, velvety
or somewhat felt-like with age. Mycelium partly immersed in the
substratum, partly supericial; hyphae branched, 2–6 μm wide,
septate, subhyaline to pale olivaceous or pale brown, smooth,
walls somewhat thickened, sometimes forming loose hyphal
aggregations, composed of swollen, subglobose hyphal cells,
pale medium brown, thick-walled, immersed hyphae often with a
slime coat, sometimes becoming spirally twisted. Conidiophores
solitary or in small groups, loose to somewhat dense, arising from
internal and external hyphae, lateral or terminal, or from swollen
hyphal cells or hyphal aggregations, erect, straight to somewhat
lexuous, cylindrical to iliform, often somewhat geniculate-sinuous
towards the apex, without multilateral swellings, unbranched, often
with short, subapical, lateral projections, sometimes branched,
once to several times, 10–300(–400) × 3–7(–8) μm, septate,
sometimes constricted at the septa, pale to medium olivaceousbrown or pale to pale medium brown, somewhat paler towards
the apex, smooth, walls somewhat thickened, often swollen and
somewhat bulbous at the base, up to 8 μm wide. Conidiogenous
cells integrated, terminal and intercalary, cylindrical or somewhat
geniculate, 18–65 μm long, proliferation sympodial, with a single
to several conidiogenous loci, subdenticulate, often situated on
small shoulders or short lateral projections, protuberant, often
truncate and almost lat, 1–2.5 μm diam, central dome usually not
higher than the surrounding rim, thickened, refractive to darkenedrefractive. Ramoconidia occasionally occurring, cylindrical-oblong,
up to 52 μm long, mostly aseptate, base 3–4 μm wide, unthickened,
not darkened, sometimes slightly refractive. Conidia catenate,
usually in branched chains, straight to slightly curved, terminal
and intercalary conidia subglobose, obovoid, ellipsoid, fusiform or
limoniform, 3–25 × 2–7(–9) μm, 0(–1)-septate, occasionally slightly
constricted at the septum, secondary ramoconidia cylindrical to
somewhat clavate or occasionally ampulliform to doliiform, up to
35 μm long, 3–7(–8) μm wide, 0–2(–3)-septate, with age slightly
constricted at the septa, subhyaline, pale brown or pale olivaceousbrown, smooth, sometimes minutely verruculose, walls somewhat
thickened, apex broadly rounded or with a single to several (up to
5) somewhat denticle-like hila, more or less truncate at the base,
hila protuberant, 1–2.5 μm diam, thickened, refractive to somewhat
darkened; microcyclic conidiogenesis sometimes observed.
In vitro: Mycelium immersed and supericial; hyphae sparingly
branched, 0.5–4 μm wide, later up to 8 μm wide, septate, with
age constricted at septa, subhyaline to very pale brown or pale
olivaceous-brown, later olivaceous-brown, smooth or almost
so, walls unthickened, broader hyphae with slightly thickened
walls. Conidiophores solitary, macro- and micronematous, arising
terminally from ascending and laterally from plagiotropous hyphae,
erect, straight to slightly lexuous, macronematous conidiophores
cylindrical-oblong, sometimes once geniculate-sinuous with
conidiogenous loci situated laterally on shoulders, non-nodulose,
usually unbranched on SNA, sometimes once branched towards
the apex (often branched on PDA according to McKemy & MorganJones, 1992), up to 350 μm long, 3–5(–5.5) μm wide, pluriseptate,
not constricted at septa, often more densely septate towards the
base, pale brown, later medium brown, smooth, walls unthickened
or slightly thickened, up to 0.5 μm wide; micronematous
conidiophores straight to slightly lexuous, iliform, narrower, 1–2
μm wide, septate, smooth. Conidiogenous cells integrated, usually
terminal, iliform to cylindrical-oblong, sometimes once geniculate,
up to 47 μm long, 1–2(–3) loci, protuberant, subdenticulate,
1.5–2 μm diam, somewhat thickened and darkened-refractive.
99
BenSch et al.
Fig. 84. Cladosporium cucumerinum (CSB 171.52, epitype). A. Symptoms on fruits of Cucumis sativus (holotype NY). B–G. Conidiophores and conidial chains in vitro. Scale
bars = 10 µm.
Ramoconidia occasionally formed, cylindrical-oblong, 24–43 ×
3–3.5 μm, 0–2-septate, smooth, base truncate, 2.5–3 μm wide,
unthickened or only slightly so. Conidia catenate, in long loosely
branched chains, often dichotomously branched, up to 10(–14)
conidia in the terminal unbranched part of the chain, straight, small
terminal conidia obovoid to ellipsoid-ovoid, subglobose, 4–8(–10)
× (1–)1.5–3(–3.5) μm (av. ± SD: 6.4 ± 1.2 × 2.2 ± 0.5), aseptate,
hila 0.5–0.8(–1) μm diam, intercalary conidia ellipsoid-ovoid to
fusiform or subcylindrical, sometimes limoniform, 7–15(–17) ×
(2–)2.5–4(–5) μm (av. ± SD: 10.9 ± 2.7 × 3.2 ± 0.6), 0(–1)-septate,
hila 0.8–1.2(–1.5) μm, secondary ramoconidia ellipsoid, fusiform to
cylindrical-oblong, (9.5–)14–30(–40) × (2.5–)3–5(–5.5) μm (av. ±
SD: 21.8 ± 5.8 × 3.8 ± 0.7), 0–2(–3)-septate, with age sometimes
slightly constricted at septa, septum median or in the upper or
lower third, pale brown or very pale brown, later becoming medium
brown, smooth, walls unthickened, slightly attenuated towards
apex and base, cells sometimes with small cavities, hila 1.5–2.2(–
2.5) μm diam, conspicuous, subdenticulate, somewhat thickened
and darkened-refractive; conidia often germinating, forming
micronematous conidiophores and conidia.
Culture characteristics: Colonies on PDA attaining 50–78 mm
diam after 14 d, grey-olivaceous to olivaceous-grey or olivaceous,
sometimes dull green towards margins and somewhat zonate,
reverse olivaceous-black, velvety to loccose or felty, margin
narrow to broad, colourless to white, regular, somewhat feathery,
aerial mycelium absent, sparse or sometimes abundantly formed
at few parts of the colony or covering almost the whole colony
surface, white, loose to dense, woolly, loccose, growth lat, regular,
without prominent exudates, sporulation profuse. Colonies on MEA
100
reaching 36–73 mm diam after 14 d, grey-olivaceous to smokegrey, whitish due to aerial mycelium, reverse iron-grey, velvety
to luffy, margin white, glabrous, regular, aerial mycelium dense,
forming mats, covering large parts of the colony surface, growth
lat, somewhat wrinkled and folded in colony centre, sometimes
with several prominent exudates, sporulation profuse. Colonies on
OA attaining 54–79 mm diam after 14 d, pale olivaceous-grey, greyolivaceous to smoke-grey or whitish, grey-olivaceous or iron-grey
at margins, reverse leaden-grey to iron-grey, velvety to loccose,
margin regular, colourless to white, glabrous, aerial mycelium
absent to abundantly formed, covering most of the colony surface,
growth lat, regular, without prominent exudates, sporulation
profuse.
Substrates and distribution: on leaves, stems and fruits of
Cucurbitaceae, especially Cucumis sativus, C. melo and Cucurbita
pepo, other host genera Citrullus, Lagenaria, Luffa, Momordica,
Sechium; cosmopolitan – Citrullus lanatus (Greece, Korea, Puerto
Rico, Romania, USA), C. vulgaris (Canada, Puerto Rico, USA,
Virgin Islands, Turkmenistan,), Coccinia grandis (Kenya), Cucumis
melo (Canada, France, Czech Republic, Great Britain, Greece,
India, Mexico, Netherlands, Panama, Romania, South Africa,
South Korea, Turkmenistan, USA, Zimbabwe), C. sativus (Armenia,
Austria, Brazil, Canada, China, Czech Republic, Denmark, Estonia,
Germany, Great Britain, Greece, Iran, Italy, Japan, Jordan,
Kenya, Latvia, Mauritius, Mexico, Netherlands, Panama, Poland,
Romania, South Africa, South Korea, Suriname, Switzerland,
USA, Zimbabwe), Cucumis spp. (Germany, Mexico, South
Korea), Cucurbita maxima (Canada, Chile, China, USA), C. pepo
(Barbados, Mauritius, New Zealand, Pakistan, South Africa, USA,
the genuS Cladosporium
Zimbabwe), Cucurbita spp. (Canada, USA), Lagenaria siceraria
(China, Greece, India), Luffa acutangula (India), L. cylindrica (= L.
aegyptiaca) (China), Momordica charantia (China), Sechium edule
(Panama), without host (Georgia, Hungary, Norway).
Additional specimens examined: Sine loco, isol. from fruits of Cucumis sativus
(Cucurbitaceae), W.W. Gilbert (CBS 108.23); isol. and ident. by J. Westerdijk, 1908
(CBS 109.08). Austria, Kärnten, Poertschack at the Wörther See, on C. sativus,
Aug. 1902, E. Cerny (BPI 426422, original material of Cladosporium cucumerinum
var. europaeum). Czech Republic, Mähren-Weisskirchen, on C. sativus, Aug.
1925, F. Petrak, Petrak, Fl. Bohem. Morav. Exs. Pilze 2108 (M-0057514). Denmark,
Kopenhagen, on C. sativus, 20 Sep. 1907, J. Lind, Flora Danica (B 700006220);
25 Sep. 1907, J. Lind, Lind, Flora Danica (B 700006219, HBG). Germany,
Bayern, Mögeldorf near Nürnberg, on C. sativus, 16 Sep. 1906, P. Magnus
(HBG); Brandenburg, Berlin, Aug. 1924, Noack (B 700006224); Versuchsfeld near
Dahlem, 20 Sep. 1902, P. Magnus (HBG); Kreis Oder-Spree, Erkner near Berlin,
18 Jun. 1892 (B 700006228, holotype of Cladosporium cucumeris); MecklenburgVorpommern, Bramow near Rostock, 18 Aug. 1924, Fr. Malchow (B 700006225);
Sachsen, Dresden-Stetzsch, on Cucumis sp., Jul. 1920, Laubert (B 700006222);
Sachsen-Anhalt, Harz, Quedlinburg, on C. sativus, 30 Jul. 1934 (B 700006221).
Netherlands, isol. by G.A. de Vries (CBS 158.51 = ATCC 11279 = IFO 6370
= IMI 049628 = VKM F-817); Amsterdam, G.W. van der Helm, isol. by G.A. de
Vries, Jan. 1951 (CBS 173.54); Berkel, G.W. van der Helm, isol. by G.A. de Vries
(CBS 174.54, CBS 175.54); Naaldwijk, isol. by S. Dudok de Wit, CBS 123.44;
Jan. 1951, G.W. van der Helm, isol. by G.A. de Vries (CBS 176.54); Sloten, coll.
by G.W. van der Helm, isol. by G.A. de Vries (CBS 172.54). Pakistan, Lahore,
on Cucurbita pepo, Dec. 1951, S. Ahmad, Reliqu. Petrak. 2342 (B 700006227,
M-0057513). Poland, Oberschlesien, Proskau, on C. sativus, 1 Sep. 1895 (B
700006226). Romania, Muntenia, District Ilfov-Bucureşti, on C. sativus, 13 Jul.
1933, T. Săvulescu & C. Sandu, Săvulescu, Herb. Mycol. Roman. 643 (M-0057515,
M-0057516). Switzerland, Graubünden, Plantahof, Landquart, on C. sativus, 23
Sep. 1901, A. Volkart (HBG). USA, Massachusetts, Clinton, on C. sativus, Oct.
1892, C.A. Chase, Massachusetts Fungi 828 (NY); Dwight, on Cucumis melo, 16
Aug. 1916, Massachusetts Fungi 2040 (NY); Pennsylvania, isol. from painted loor
by M.H. Downing, 1951 (CBS 174.62 = ATCC 16022 = ATHUM 2861 = CECT
2110 = IFO 31006 = IMI 045534 = MUCL 19019 = QM 489 = VTT D-92188).
Notes: Cladosporium cucumerinum is a biotrophic species conined
to hosts of the Cucurbitaceae. In phylogenetic analyses, this species
clustered as a sister to C. subuliforme (Bensch et al. 2010, ig. 1,
part c), but formed a distinct lineage. Morphologically it resembles
C. cladosporioides, but differs in having geniculate conidiophores
and somewhat wider conidia. The characters of the two species
in vitro are also distinct. In de Vries (1952) and McKemy &
Morgan-Jones (1992) “Macrosporium cucumerinum Ellis & Everh.,
Hedwigia 7: 49. 1896.” is cited as a synonym of C. cucumerinum,
but in Hedwigia, vol. 7 (published in 1868, not in 1896) there is
no reference to this name. In Index Fungorum the original citation
of Macrosporium cucumerinum is given as “Proc. Acad. Nat. Sci.
Philadelphia 1895: 440. 1895.” and “Alternaria cucumerina (Ellis
& Everh.) A. Elliott, Amer. J. Bot. 4: 472. 1917.” is given as current
name. De Vries (1952) listed Chloridium polysporum (≡ Acladium
polysporum) as an additional synonym and stressed that this name
antedated C. cucumerinum. McKemy & Morgan-Jones (1992)
discussed this “synonymy” and stated that the two species are
not identical and do not even belong in the same genus. Hughes
(1958), who examined type material of Wallroth’s species, reduced
A. polysporum to synonymy with Botrytis cinerea.
Cladosporium cucumerinum, the causal organism of crown
blight and scab or gummosis disease is widespread and common
especially on Citrullus lanatus, Cucumis melo, C. sativus and
Cucurbita pepo, but various records from other members of the
Cucurbitaceae belong very probably to this species and are,
therefore, listed under “substrates and distribution”. However,
there are also records on hosts belonging to other families. These
records are partly doubtful, but may indicate that this species is not
conined to cucurbits. Molecular analyses based on material from
hosts of other plant families are necessary to solve this problem.
www.studiesinmycology.org
Roberts et al. (1986) examined the internal mycolora of achenes
of Helianthus annuus (Asteraceae) and reported C. cucumerinum
as isolated from developing sunlower seeds. Morphological data
and illustrations have not been provided, and cultures could not be
traced, so that a veriication of the identity of the fungus concerned
was not possible. Hasija (1967) described this species from India
on Solanum tuberosum (Solanaceae). In Korea, a Cladosporium
species was isolated from leaves of Solanum melongena cultivated
in a greenhouse, and identiied as C. cucumerinum by Kwon et al.
(1999). They carried out inoculation experiments and reported it to
cause symptoms in seedlings of watermelon, cucumber, oriental
melon and pumpkins. Kwon et al. (2000) reported it to cause a
black scab disease on sword bean (Canavalia ensiformis = C.
gladiata, Fabaceae) in greenhouses in Korea. Mendes et al. (1998)
listed Capsicum annuum as a further host species. An isolate from a
painted loor in the USA very probably resulted due to contamination
with C. cucumerinum (Bensch et al. 2010). The species was
originally described from North America but an epitypiication with
European material is justiied due to its pathogenicity to several
hosts of cucurbits and its cosmopolitan distribution.
40. Cladosporium cycadis Marcolongo, Riv. Patol. Veg.,
Ser. 2, 7(1): 8. 1914. Fig. 85.
Neotype (designated here): Italy, Orto Botanico di Napoli, on
Cycas revoluta (Cycadaceae), I. Marcolongo, Cavara & Pollacci,
Fung. Paras. Piante Colt. Utili Exs. 439 (BPI 426433). Isoneotype:
M-0057511.
Lit.: Saccardo (1931: 790), Rao & Baheker (1964), Zhang et al.
(1998a: 6, 2003: 82–83).
Ill.: Zhang et al. (1998a: 5, ig. 2; 2003: 82, ig. 46).
Exs.: Cavara & Pollacci, Fung. Paras. Piante Colt. Utili Exs. 439;
Ciferri, Mycol. Doming. Exs. 392.
In vivo: On living and faded leaves, causing dingy olivaceous-green
to brown lesions, small to large leaf segments becoming necrotic,
size and shape variable, margin indeinite, later sometimes entire
leaves turning brown. Colonies faintly punctiform to effuse, often
Fig. 85. Cladosporium cycadis (BPI 426433). Conidiophores and conidia in vivo.
Scale bar = 10 µm. U. Braun del.
101
BenSch et al.
rather inconspicuous, dark brown. Mycelium internal. Stromata
lacking or small, up to 50 μm diam, brown, immersed to slightly
erumpent. Conidiophores solitary or in small, usually loose fascicles,
arising from internal hyphae, swollen hyphal cells or stromata,
erect, erumpent, straight, subcylindrical to usually geniculatesinuous, often strongly so, sometimes subnodulose, unbranched
or branched, 20–60 × 2–6 μm, pluriseptate throughout, olivaceousbrown, smooth, wall thin, ca. 0.5 μm wide. Conidiogenous cells
integrated, terminal and intercalary, 10–25 μm long, conidiogenous
loci 1–1.5 μm diam, slightly protuberant. Conidia catenate, in
simple or branched chains, subglobose, ellipsoid-ovoid, fusiform,
subcylindrical, 4–10 × 2–5 μm, secondary ramoconidia 6–24 × 3–7
μm, 0–2(–3)-septate, subhyaline to pale olivaceous or olivaceousbrown, smooth, thin-walled, up to 0.5 μm, ends obtuse to attenuated,
hila often somewhat protuberant, subdenticulate, 1–1.5 μm diam,
distinctly coronate; microcyclic conidiogenesis not occurring.
Substrates and distribution: On Cycas spp. (Cycadaceae); Asia,
Europe, Central America – C. intermedia (Czech Republic), C.
revoluta (China, Dominican Republic, Italy), C. rumphii (China).
Additional specimens examined: Czech Republic, Moravia, “Eisgrub”, on Cycas
intermedia, H. Zimmermann, ex herb. Bubák, 20 Feb. 1909 (BPI 426121).
Dominican Republic, Prov. Santo Domingo, Llano Costero, Ciudad Trujillo, on
Cycas revoluta, Jul. 1928, R. Ciferri, Ciferri, Mycol. Doming. Exs. 392 (BPI 426432).
Fig. 86. Cladosporium delectum [K (F) 121550]. Conidiophores and conidia in vivo.
Scale bar = 10 µm. U. Braun del.
Notes: Cladosporium apicale differs in having much longer
conidiophores.
Substrate and distribution: On dead leaves of Magnolia virginiana;
USA.
41. Cladosporium delectum Cooke & Ellis, Grevillea 6(37):
6. 1877. Fig. 86.
Lectotype (selected here): USA, New Jersey, Newield, on dead
leaves of Magnolia virginiana (= M. glauca) (Magnoliaceae), J.B.
Ellis 3038 [ex herb. Cooke 1885] [K (F) 121550].
Lit.: Saccardo (1886: 358), Lindau (1907: 822), Ferraris (1912:
341), Cash (1952: 68).
Ill.: Cooke & Ellis (1877: pl. 96, ig. 36).
Exs.: Ellis, N. Amer. Fungi 371; Ellis & Everhart, Fungi Columb.
164.
In vivo: Saprobic on dead leaves (leaf litter). Colonies punctiform
to effuse, blackish brown. Mycelium immersed. Stromata lacking.
Conidiophores solitary or in small to moderately large, loose fascicles,
arising from immersed hyphae or small aggregations of swollen hyphal
cells, erect, straight to usually strongly geniculate-sinuous, but not
nodulose, unbranched or occasionally branched, 50–300 × 3–8 μm, at
the very base up to 12 μm wide, pluriseptate throughout, medium to
medium dark brown, smooth or almost so, wall thickened, 0.5–1.5(–2)
μm wide, often distinctly two-layered, above all below. Conidiogenous
cells integrated, terminal and intercalary, 10–25 μm long, with a single or
several conidiogenous loci, only slightly protuberant, 1–2 μm diam, raised
periclinal rim not very evident when viewed by light microscopy. Conidia
catenate, in simple or branched chains, ramoconidia and secondary
ramoconidia present, 8–18 × 3–7 μm, 0–3-septate, ramoconidia with
truncate base, 2–4 μm wide, without coronate hilum, conidia ellipsoidovoid, fusiform, subcylindrical, 3–15 × 2.5–5.5 μm, 0–1-septate,
subhyaline to pale olivaceous or olivaceous-brown, smooth or almost
so, thin-walled (< 1 μm), ends rounded to attenuated, hila 1–2 μm diam.
102
Additional specimens examined: USA, New Jersey, Newield, on dead leaves of
Magnolia virginiana, June 1878, Ellis, N. Amer. Fungi 371(NY) and June 1893, Ellis
& Everhart, Fungi Columb. 164 (NY), topotype material.
Notes: Zhang et al. (2003) recorded, described and illustrated this
species from China on leaves of Castanea mollissima. However,
the Chinese collection does not agree with type material of C.
delectum. Some duplicates of Fungi Columb. 164 contain mixtures
of Cladosporium herbarum, C. cladosporioides and Alternaria sp.
42. Cladosporium delicatulum Cooke, Grevillea 5(33): 17.
1876. Figs 87–92.
Holotype: India, on dead leaves (litter), Colonel Hobsen, No.
23 (K [M] 121551). Isotypes: Vize, Micro-Fungi Exot. 24 (e.g., B
700006230).
= Cladosporium fasciculatum f. scirpi-lacustris Roum., Fungi Sel. Gall. Exs.,
Cent. 17, No. 1688. 1881, nom. nud. [syntypes: e.g. FH].
= Cladosporium fasciculatum var. densum Ravenel, Fungi Amer. Exs., Cent.
VII, No. 602. 1882, nom. nud. [syntype: BPI 426554]
= Cladosporium tuberum Cooke, Grevillea 12(61): 31. 1883 [lectotype: K].
Lit.: Saccardo (1886: 361–362), Bensch et al. (2010: 37–40).
Ill.: Bensch et al. (2010: 38–40, igs 22–25).
Exs.: Anonymous, Calif. Fungi 427; Herter, Plantae Urug. Exs.
1496; Ravenel & Cooke, Fungi Amer. Exs. 600, 602; Roumeguère,
Fungi Sel. Gall. Exs. 1688; Thümen, Mycoth. Univ. 1767.
Description based on poor type material: Saprobic, on dead
leaves, forming punctiform colonies, effuse, dark brown to blackish.
Mycelium immersed. Conidiophores solitary or loosely aggregated,
the genuS Cladosporium
Fig. 89. Cladosporium delicatulum (K, lectotype of C. tuberum). Conidiophores and
conidia in vivo. Scale bar = 10 µm. U. Braun del.
Fig. 87. Cladosporium delicatulum. Conidiophores and conidia in vivo. Scale bar =
10 µm. U. Braun del.
Fig. 88. Cladosporium delicatulum [K (M) 121551]. Conidiophores and conidia in
vivo. Scale bar = 10 µm. U. Braun del.
caespitose, arising from immersed hyphae or aggregations
of swollen, brown hyphal cells, 3–8 μm diam, erect, straight,
subcylindrical-conical to usually distinctly geniculate-sinuous,
www.studiesinmycology.org
usually unbranched, rarely once branched, 20–100 × 3–5.5 μm,
pluriseptate, pale to medium dark brown, smooth to rough-walled,
walls thickened, 0.5–1.5 μm wide. Conidiogenous cells integrated,
terminal and intercalary, 10–30 μm long, conidiogenous loci
conspicuous, coronate, 1–2 μm diam, somewhat protuberant.
Conidia catenate, in simple or branched chains, 5–15 × 2.5–6 μm,
terminal conidia obovoid, aseptate, intercalary conidia ellipsoidfusiform, 0–1-septate, secondary ramoconidia ellipsoid-fusiform,
subcylindrical, 0–1(–2)-septate, pale olivaceous to olivaceousbrown, smooth or almost so, thin-walled, but often with distinctly
reduced lumen, with a single basal and 1–2 terminal hila, 1–1.5
μm diam.
Variation of other collections in vivo: Saprobic on dead leaves, fruits,
stems, tubers or necrotic lesions caused by other fungi. Colonies
punctiform, pustulate to effuse, forming loose to dense patches or
covers, dingy dark brown to usually blackish. Mycelium immersed;
hyphae simple or branched, 2–7 μm wide, septate, olivaceous
to brown, wall thin to slightly thickened, smooth or almost so,
forming strands and small to well-developed, stromatic hyphal
aggregations, loose to dense, immersed to somewhat erumpent,
composed of swollen hyphal cells 2.5–12 μm diam, olivaceous,
olivaceous-brown to dark brown, wall thin to somewhat thickened.
Conidiophores solitary or loosely aggregated, caespitose to almost
fasciculate, arising from immersed hyphae or stromatic hyphal
aggregations, on leaves erumpent or emerging through stomata,
erect to decumbent, straight, subcylindrical to geniculate-sinuous,
occasionally subnodulose, simple or occasionally branched, 10–200
× 3–7 μm wide, septate, usually pluriseptate throughout, sometimes
constricted at the septa, pale to dark olivaceous-brown, brown to
medium dark brown, sometimes paler towards the apex, wall thin
to somewhat thickened, usually thick below and thinner towards
the apex, 0.5–1.5(–2) μm, occasionally distinctly two-layered near
the base, smooth to rough-walled. Conidiogenous cells integrated,
terminal, sometimes also intercalary, 8–32 μm long, conidiogenous
loci 1–2 μm diam, slightly protuberant, differentiation of the
raised periclinal rim often not very distinct, somewhat darkenedrefractive. Conidia in simple and branched chains, ellipsoid-ovoid,
obovoid, fusiform, subcylindrical, rarely subglobose, 3–25(–30) ×
(2–)3–6(–7) μm, 0–3-septate, pale to medium olivaceous-brown
or brown, smooth or almost so, occasionally smooth and roughwalled conidia mixed (above all small, aseptate conidia sometimes
verruculose), cells sometimes with distinct lumen giving the conidia
103
BenSch et al.
Fig. 90. Cladosporium delicatulum (CBS H-20430, reference material). Conidiophores and conidia in vivo. Scale bar = 10 µm. K. Bensch del.
Fig. 91. Cladosporium delicatulum (CBS 126344). Macro- and micronematous conidiophores and conidial chains in vitro. Scale bar = 10 µm. K. Bensch del.
104
the genuS Cladosporium
Fig. 92. Cladosporium delicatulum (CBS 126344). A–G. Macronematous conidiophores and conidial chains. Scale bar = 10 µm.
a rather thick-walled appearance, ends rounded to attenuated,
wall thin (≤ 1 μm), hila 1–2 μm diam, barely to slightly protuberant,
occasionally somewhat denticle-like; germination and microcyclic
conidiogensis occurring.
In vitro: Mycelium immersed, rarely supericial; hyphae unbranched
or sparingly branched, (0.5−)1−3(−4) μm wide, septate, without
swellings and constrictions, subhyaline to pale olivaceous
or pale olivaceous-brown, smooth to minutely verruculose,
sometimes loosely verrucose. Conidiophores macronematous
and micronematous, solitary, arising terminally and laterally from
hyphae, erect, straight to somewhat lexuous, cylindrical-oblong,
non-nodulose, sometimes slightly geniculate towards the apex,
unbranched, occasionally branched, once or several times, often
as short peg-like prolongations, 50−165(−200) × 3−4.5(−5) μm,
2−4(–7)-septate, sometimes attenuated at septa, pale olivaceous
to pale medium olivaceous-brown, smooth, sometimes loosely
minutely verruculose at the base, walls unthickened or almost so,
about 0.5 μm wide, sometimes slightly attenuated towards the
apex, up to 5.5 μm wide at the base; micronematous conidiophores
narrower and pale olivaceous, 19−75(−100) × (1.5−)2−2.5 μm.
Conidiogenous cells integrated, terminal, sometimes intercalary,
situated on small peg-like prolongations, cylindrical-oblong,
sometimes geniculate at or towards the apex, non-nodulose,
occasionally the whole cell inlated in shape like a secondary
ramoconidium, 11−37 μm long, with (1−)2−3(−4) apical loci, crowded
at the apex, conspicuous, subdenticulate to denticulate, sometimes
situated on small lateral outgrowths, quite broad, truncate, rim
and dome not distinctly visible, 1.5−2.2 μm diam, thickened and
darkenedrefractive. Ramoconidia cylindrical-oblong, 13−46 ×
www.studiesinmycology.org
2.5−4(−5) μm, 0−1(−2)-septate, sometimes distinctly constricted at
the median septum, base broadly truncate, 2−3 μm wide, neither
thickened nor darkened-refractive. Conidia numerous, in densely
branched chains, branching in all directions, up to four conidia in
the terminal unbranched part of the chain, small terminal conidia
obovoid, subglobose or globose, 2.5−4.5(−6) × (1.5−)2−2.5(−3.5)
μm (av. ± SD: 3.7 ± 0.8 × 2.4 ± 0.4), aseptate, apex rounded,
sometimes irregular due to additional lateral hila, intercalary conidia
limoniform to ellipsoid-ovoid or sometimes irregular in outline due
to lateral hila, 4−13(−17.5) × 2.5−3.5(−4) μm (av. ± SD: 7.8 ± 3.0
× 3.0 ± 0.4), 0−1-septate, attenuated towards apex and base,
with 1−4(−6) distal hila, secondary ramoconidia ellipsoid-ovoid to
subcylindrical or cylindrical, (6−)8−23.5(−31) × (2.5−)3−4.5(−5)
μm (av. ± SD: 15.6 ± 5.4 × 3.6 ± 0.5), 0−1(−2)-septate, very
rarely 3-septate, not constricted at septa, pale olivaceous to pale
olivaceous-brown, smooth or almost so, walls unthickened, often
only slightly attenuated towards apex and base, with (1−)2−4(−5)
distal hila, hila conspicuous, subdenticulate or denticulate,
0.5−2.2 μm diam, thickened and darkened-refractive; microcyclic
conidiogenesis not observed.
Culture characteristics: Colonies on PDA attaining 60−78 mm
diam after 14 d, olivaceous-grey, grey-olivaceous to olivaceous
and olivaceous-black, reverse olivaceous-black, loccose to
villose, margins grey-olivaceous, feathery, regular, aerial mycelium
scattered to abundant, covering almost the whole colony surface,
loccose to villose, low to rarely high, growth lat, without prominent
exudates, sporulation sparse. Colonies on MEA reaching 67−76 mm
diam after 14 d, smoke-grey to pale olivaceous-grey, olivaceousgrey or glaucous-grey at margins, reverse olivaceousgrey, loccose,
105
BenSch et al.
luffy, margins white, glabrous to feathery, regular, aerial mycelium
abundant, covering the whole colony surface, loccose to luffy,
growth lat, radially furrowed and wrinkled in colony centre, without
prominent exudates, sporulation sparse or absent. Colonies on OA
reaching 55−74 mm diam after 14 d, smoke-grey to pale olivaceousgrey, grey-olivaceous or olivaceous due to abundant sporulation,
reverse pale greenish grey to olivaceous-grey, velvety to loccose,
margins regular, glabrous, narrow, colourless, aerial mycelium
sparse to abundant, covering the whole surface, loccose, loose
to dense, low, growth lat, without prominent exudates, sporulation
sparse to profuse.
Substrates and distribution: Isolated from air, building material, dust,
plant material; Europe (Denmark, Germany). Saprobic on dead
leaves, fruits, stems, tubers, or occurring as secondary invader on
necrotic lesions caused by other fungi in vivo, widely distributed,
Asia (China, incl. Taiwan, India), Europe (France, Germany), North
America (USA), South America (Uruguay).
Additional specimens examined: Denmark, isol. from indoor air, 2007, B. Andersen
(BA 1679 = CPC 14285, BA 1680 = CPC 14286, BA 1681 = CBS 126342 = CPC
14287); isol. from building material, school, 2007, B. Andersen (BA 1698 = CBS
126343 = CPC 14299); isol. from building material, 2007, B. Andersen (BA 1683
= CPC 14289); Broenshoej, isol. from indoor air, control room, 2000, B. Andersen
(BA 1724 = CPC 14363); indoor air sample, in cup board, water damaged room,
2000, B. Andersen (BA 1718 = CPC 14360); beach near Copenhagen, isol. from
seaweed, 2007, B. Andersen (BA 1706 = CPC 14307); Valleroed, isol. from dust,
school, 2000, B. Andersen (BA 1740 = CPC 14372). France, Lyon, on dead
stems of Schoenoplectus lacustris (= Scirpus lacustris) (Cyperaceae), 1880, J.
Therry, Roumeguère, Fungi Sel. Gall. Exs. 1688 (HBG), and Thümen, Mycoth.
Univ. 1767 (HAL, HBG, syntypes of C. fasciculatum f. scirpi-lacustris). Germany,
Bayern, Hitzing, on dead leaves of Hedera helix (Araliaceae), 15 Oct. 1915, Niessl
(M-0057550); München, park of castle Nymphenburg, isol. from Puccinia bromina
ssp. symphyti-bromarum var. paucispora, Jul. 2006, K. Schubert (CPC 13148);
Sachsen-Anhalt, Halle (Saale), Robert-Franz-Ring, isol. from leaves of Tilia cordata
(Tiliaceae), 2 Aug. 2004, K. Schubert (CBS H-20430, CBS 126344 = CPC 11389,
reference strain of C. delicatulum). India, Gorakhpur, on necrotic patches on faded
leaves of Dianthus barbatus (Caryophyllaceae), 23 Mar. 1972, Y.N. Srivastava, No.
2 (IMI 212469) (originally deposited as C. cladosporioides). USA, California, Marin
County, San Rafael, on leaves of Hedera helix, as secondary invader, 11 Nov. 1935,
L. Bonar, Anonymous, Calif. Fungi 427 (NY); South Carolina, Aiken, on faded and
necrotic leaves of Euonymus japonicus (Celastraceae), Ravenel, Ravenel & Cooke,
Fungi Amer. Exs. 602 (BPI 426554, syntype of C. fasciculatum var. densum); on
tubers of Ipomoea batatas (Convolvulaceae), Ravenel, Ravenel & Cooke, Fungi
Amer. Exs. 600 (K, lectotype of C. tuberum designated here). Uruguay, on dead
pods of Sesbania virgata (= S. marginata) (Fabaceae), June 1932, S. José, Herter,
Plantae Urug. Exs. 1496 (HBG).
Notes: In phylogenetic analyses, Cladosporium delicatulum
clustered as a sister to C. cladosporioides s. lat. (Bensch et al.
2010, ig. 1, part a) and formed a distinct lineage. This species
is undoubtedly a common and widespread saprobic hyphomycete.
Cladosporium inversicolor and C. cladosporioides are two
comparable taxa, but C. delicatulum differs from the latter species in
having 0−1-septate intercalary conidia and secondary ramoconidia,
only a few conidia in the terminal unbranched part of conidial
chains, shorter often slightly geniculate conidiophores and shorter
secondary ramoconidia. Cladosporium inversicolor is distinct by
its longer conidial chains, longer small terminal and intercalary
conidia, wider intercalary conidia and secondary ramoconidia,
longer ramoconidia with a broader base, with conidia being smooth
to loosely verruculose or irregularly rugose. The old, sparse type
material of C. delicatulum is from India. New Indian collections
and cultures are not available. Therefore, a formal epitypiication of
this species is not yet proposed, but the German strain from Tilia
cordata can serve as reference strain to ix the application of C.
delicatulum and agrees well with the Indian type material.
106
43. Cladosporium diaphanum Thüm., Mycoth. Univ., Cent.
XIX, No. 1868. 1881. Figs 93–95.
Lectotype (selected by Braun 2001): France, Lyon, on dead leaves
of Photinia glabra (Rosaceae), Jun. 1880, J. Therry, Thümen,
Mycoth. Univ. 1868 (HAL). Isolectotypes: M-0057506 and Thümen,
Mycoth. Univ. 1868 (e.g., B 700006244, BPI 426451, BPI 426452,
HBG, M-0057507). Topotypes: France, Rhone-Alpes, Rhone, Lyon,
Parc de la Tete-d’Or, on Photinia glabra, J. Therry, Roumeguère,
Fungi Sel. Gall. Exs. 5591 (e.g., B 700006247).
Lit.: Saccardo (1892: 603), Ellis (1976: 342), Braun (2001: 56),
Schubert (2005b: 64–67).
Ill.: Ellis (1976: 341, ig. 259 B), Braun (2001: 55, ig. 2), Schubert
(2005b: 66, ig. 21, pl. 10, igs A–G).
Exs.: Roumeguère, Fungi Sel. Gall. Exs. 5591; Thümen, Mycoth.
Univ. 1868.
In vivo: On faded or dead leaves and petioles, without distinct leaf
spots, causing whitish to pale greyish discolorations, membranous.
Colonies usually hypophyllous, sparsely developed on the upper leaf
surface, caespitose, punctiform to effuse, loose to dense, olivaceousbrown to brown or even blackish brown, velvety, often covering large
areas or almost the entire leaf surface. Mycelium internal, mostly
substomatal, subcuticular to intraepidermal; hyphae sparingly
branched, 3–6 μm wide, septate, sometimes slightly constricted at
the septa, subhyaline to pale brown, smooth, walls slightly thickened.
Stromata or stromatic hyphal aggregations small to extended, mostly
several layers deep, composed of subglobose to somewhat angular-
Fig. 93. Cladosporium diaphanum (M-0057507). Fascicle of conidiophores
emerging through stomata and conidia in vivo. Scale bar = 10 µm. K. Bensch del.
the genuS Cladosporium
Fig. 95. Cladosporium diaphanum (M-0057507). A. Overview. B. Branched
conidiophore and conidia. C. Conidium showing coronate scar structure. D.
Branched conidiophores. E. Tips of conidiophores and conidia. F. Base of a fascicle
emerging through stomata. G. Conidiophore showing cell structure, with thickened,
two-layered walls and protoplasm aggregated at the septa. Scale bars = 5 (C), 10
(E, G), 20 (B, D, F), 50 (A) µm.
Fig. 94. Cladosporium diaphanum (B 700006247). Conidiophores and conidia in
vivo. Scale bar = 10 µm. K. Bensch del.
oblong, polygonal cells, 5–9 μm wide, pale to medium or even dark
brown, smooth, slightly thick-walled. Conidiophores solitary or often
fasciculate, in loose to dense fascicles, arising from stromata or
swollen hyphal cells, usually emerging through stomata, but also
erumpent through the cuticle, erect to decumbent, straight to lexuous,
iliform-setiform, not or somewhat geniculate-sinuous towards the
apex, not or hardly nodulose, unbranched or often basely or apically
branched, once to several times (mostly unilateral), 40–250(–300) ×
(3–)4–7(–8) μm, pluriseptate, sometimes slightly constricted at the
septa, medium brown to medium dark golden brown, base often
somewhat darker, paler towards the apex, smooth, occasionally
faintly rough-walled, thick-walled, sometimes distinctly two-layered,
often somewhat wider or swollen at the base, up to 11(–13) μm wide,
attenuated towards the apex, protoplasm of the cells somewhat
aggregated at the septa, which appear to be thickened, similar to
distoseptation, pale and not very conspicuous. Conidiogenous cells
integrated, terminal and intercalary, 7–40 μm long, proliferation
sympodial, with one to several conidiogenous loci, more or less
protuberant, 1–2.5(–3) μm diam, dome only somewhat higher than
the surrounding rim, thickened, darkened-refractive. Ramoconidia
occasionally observed. Conidia catenate, in unbranched or branched
chains, mostly straight, dimorphic, small aseptate conidia subglobose,
ovoid, limoniform, ellipsoid, fusiform, (2–)3–14 × 2–6 μm, pale, walls
thin to only slightly thickened, smooth to faintly rough-walled, larger
www.studiesinmycology.org
conidia obovoid, ellipsoid, fusiform to cylindrical, 9–45 × 4–8 μm,
(0–)1–6-septate, sometimes slightly constricted at the septa, pale
olivaceous to medium brown, smooth to minutely verruculose, walls
slightly thickened, apex rounded or slightly attenuated towards the
apex and base, hila protuberant, 1–2.5(–3) μm diam, thickened,
darkened-refractive; microcyclic conidiogenesis occurring.
Substrates and distribution: On Photinia glabra; France.
Notes: Conidiophores in lectotype and isolectotype material
are often once or several times branched, whereas those in the
authentic material distributed as “Roumeguère, Fungi Sel. Gall.
Exs. 5591” are usually unbranched. Cladosporium diaphanum
was collected in June on faded or dead leaves of Photinia glabra,
but it remains unclear, whether this species caused the premature
dying and leaf fall or whether it is a saprobic species. Additional
collections are necessary to clarify the biology of this taxon.
Among the Cladosporium species occurring on leaves of various
host genera and families some morphologically similar species with
pluriseptate (more than three septa) conidia and non-nodulose
conidiophores are easily distinguishable from C. diaphanum in
having wider conidia [5–14(–17) μm wide in C. coryphae], shorter
and partly narrower, usually unbranched conidiophores (35–130
μm long in C. bosciae; 5–90 × 3–5 μm in C. spongiosum), narrower
conidiogenous loci (usually 0.5–1.5 μm diam in C. spongiosum)
and differ in the formation and arrangement of conidiophores
107
BenSch et al.
(conidiophores solitary, arising as terminal or lateral branches of
creeping hyphae in C. spongiosum; conidiophores arising from
external mycelium in C. coryphae; conidiophores solitary or in
loose groups arising from intraepidermal stromata or from swollen
hyphal ropes in C. bosciae). The widespread saprobic C. herbarum
is quite distinct in having verruculose to verrucose, 0–3-septate,
shorter conidia and nodulose conidiophores with conidiogenous
loci restricted to swellings.
In absence of a monograph of the genus Cladosporium, Braun
(2001) assigned some collections on several basidiomycetes to
C. diaphanum. During the course of a morphotaxonomic revision
of fungicolous Cladosporium species these collections were
re-examined and proved to be identical with C. lycoperdinum
(Heuchert et al. 2005). The conidia of the latter species are shorter,
(2–)6–28 μm long, 0–3-septate, narrow ramoconidia with up to 5
septa (21–32 × 4.5–5.5 μm) are occasionally formed and the walls
of the conidiophores are only slightly thickened and not distinctly
two-layered.
Cladosporium diaphanum has been recorded in literature from
Georgia to occur on Laurocerasus oficinalis, which could not be
conirmed.
44. Cladosporium dieffenbachiae Verpl. & Van den
Broecke, Ann. Soc. Sci. Bruxelles, Sér. B, 56: 105. 1936. Fig.
96.
Holotype: Belgium, Gent, botanical garden, on dead leaves of
Dieffenbachia seguine (= D. magniica) (Araceae), Sep. 1935,
associated with Septoria dieffenbachiae (GENT).
In vivo: Colonies hypophyllous, effuse, dense, dark olivaceousbrown or brown, often along leaf veins. Mycelium internal and
external; hyphae branched, 2–7 μm wide, septate, with swellings
and constrictions, pale olivaceous-brown to medium brown,
smooth, rarely minutely verruculose, forming loose to somewhat
dense hyphal aggregations. Stromata lacking. Conidiophores
solitary, arising from creeping hyphae or swollen hyphal cells,
terminal and lateral, more or less erect, straight to slightly lexuous,
dimorphic, small conidiophores unbranched, sometimes with
small swellings, 8–30 × 3–5 μm, pale brown, large conidiophores
distinctly nodulose, unbranched or branched, once or several
times, 35–170 × 3–6 μm, swellings up to 10 μm wide, septate,
pale brown to pale medium brown, somewhat paler towards the
apex, smooth, walls thickened. Conidiogenous cells integrated,
terminal and intercalary, nodulose, conidiogenous loci situated
on multilateral swellings, with a single or few loci, somewhat
protuberant, (0.5–)1–2 μm diam, thickened, slightly darkenedrefractive. Conidia in branched chains, straight to slightly curved,
small terminal conidia subglobose, ovoid, obovoid or limoniform,
3–7 × 2–4.5 μm, aseptate, smooth to verruculose, sometimes even
verrucose, large conidia (secondary ramoconidia) subglobose,
ellipsoid, subcylindrical to cylindrical, 4–27 × 3–6.5 μm,
0–6-septate, not constricted, olivaceous to medium pale brown,
smooth to verruculose, walls slightly thickened, apex rounded or
somewhat attenuated, often with few apical conidiogenous hila,
subdenticulate, truncate to slightly convex, 0.5–1.5(–2) μm diam,
thickened, somewhat darkened-refractive.
Substrates and distribution: On Dieffenbachia seguine; Belgium.
108
Fig. 96. Cladosporium dieffenbachiae (GENT). Conidiophores and conidia in vivo.
Scale bar = 10 µm. K. Bensch del.
Notes: The biology of C. dieffenbachiae is unclear, but it is very
probably a saprobic species. Due to nodulose conidiophores, it
is comparable with C. oxysporum and the C. herbarum complex.
Cladosporium oxysporum differs from this species in having much
longer, setiform, non-dimorphic conidiophores and consistently
smooth-walled, usually 0–1-septate conidia. Within the C.
herbarum complex C. dieffenbachiae agrees well with C. allicinum,
but the conidia are up to 6-septate. Furthermore, the conidiophores
of the latter species are not dimorphic in vivo. Therefore, C.
dieffenbachiae is tentatively maintained as a separate species.
Cultures and molecular sequence analyses are urgently necessary.
A single collection under the name C. dieffenbachiae from Mexico
on leaves of Dieffenbachia seguine (as D. picta) is deposited at
BPI.
45. Cladosporium dominicanum Zalar, de Hoog & GundeCimerman, Stud. Mycol. 58: 169. 2007. Fig. 97.
Holotype: Dominican Republic, salt lake Enriquillo, isol. from
hypersaline water, Jan. 2001, N. Gunde-Cimerman, isol. P. Zalar
(CBS H-19733). Ex-type culture: EXF-732 = CBS 119415.
Ill.: Zalar et al. (2007: 170, ig. 6).
In vitro: Mycelium unbranched to sparingly branched, septate, not
constricted at septa, pale olivaceous-brown, minutely verruculose
to irregularly rough-walled, walls unthickened or almost so,
protoplasm somewhat aggregated in the centre of the cells, granular,
without extracellular polysaccharide-like material. Conidiophores
micronematous and semimacronematous, hardly distinguishable
from hyphae, arising laterally and terminally on erect or ascending
hyphae, erect, somewhat lexuous, iliform to cylindrical-oblong,
usually neither geniculate nor nodulose, unbranched or branched,
once or several times, branches as short lateral prolongations
below a septum, (5–)10–100(–200) × (1–)2–2.5(–3.5) μm, aseptate
or with few septa, pale olivaceous-brown, smooth to minutely
the genuS Cladosporium
Fig. 97. Cladosporium dominicanum (CBS 119415) (from Zalar et al. 2007). A–D. Colony surface grown on PDA (A), OA (B), MEA (C) and MEA plus 5 % NaCl (D) of strains
incubated for 14 d at 25 ºC in darkness. E–F. Habit of conidiophores. G. Conidiophore. H–I. Secondary ramoconidia and conidia. E–I. All from 7-d-old SNA slide cultures. A, D,
F–H, from EXF-2519; B, C, E from EXF-727; I, EXF-732 (ex-type strain). Scale bars = 10 (G–I), 30 (F), 100 (E) µm.
verruculose, walls thin-walled to slightly thickened; micronematous
conidiophores often only as short denticle- or peg-like lateral
outgrowth of hyphae. Conidiogenous cells integrated, teminal,
sometimes intercalary or conidiophores reduced to conidiogenous
cell, cylindrical, with a single or few apical loci, protuberant,
www.studiesinmycology.org
denticulate, 0.8–1.5 μm diam, thickened and darkened-refractive.
Ramoconidia rarely formed. Conidia catenate, in branched chains,
branching in all directions, up to eight conidia in the unbranched
parts, small terminal conidia globose or subglobose to usually
short-ovoid, narrower at both ends, (2–)3–3.5(–5.5) × 2–2.5 μm
109
BenSch et al.
[av. (± SD) 3.4 (± 0.6) × 2.2 (± 0.2)], aseptate, smooth to minutely
verruculose, intercalary conidia ovoid, limoniform to ellipsoid, 3–8.5
× 2–3 μm, aseptate, smooth to minutely verruculose, with up to three
distal hila, secondary ramoconidia cylindrical to almost spherical,
attenuated towards apex and base, 6.5–24.5 × (2–)2.5–3(–4.5) μm,
[av. (± SD) 10.3 (± 5.2) × 2.7 (± 0.6)], 0–1-septate, not constricted at
the median septum, with up to four distal scars, subhyaline to pale
olivaceous or light brown, smooth or almost so, walls unthickened
to slightly thickened, hila protuberant, conspicuous, denticulate,
0.5–1.5 μm diam, thickened and darkenend-refractive; microcyclic
conidiogenesis not occurring.
Culture characteristics: Colonies on PDA reaching 18–36 mm
diam after 14 d, grey-olivaceous in colony centre due to abundant
sporulation, glaucous-grey to greenish-grey, reverse greenish-grey,
velvety to hairy or felty, margin regular, white, somewhat feathery,
aerial mycelium aundant, high, luffy to felty, covering most of the
surface, lat or slightly furrowed, with lat margin, numerous small
droplets of light reseda-green (2E6) exudates sometimes present.
Colonies on OA reaching 19–34 mm diam after 14 d, dark mousegrey, reverse black, velvety to loosely powdery with raised central
part due to fasciculate bundles of conidiophores, aerial mycelium
sparse, whitish to smoke-grey, without exudates, sporulating.
Colonies on MEA reaching 30–32 mm diam after 14 d, reseda green
(2E6), reverse dark green-brown, velvety, furrowed, with undulate
margin. Colonies on MEA + 5 % NaCl reaching 37–41 mm diam,
reseda-green (2E6), reverse brownish green, radially furrowed,
velvety, sporulating in the central part or all over the colony, margin
white and regular. Maximum tolerated salt concentration: 75 % of
tested strains develop colonies at 20 % NaCl after 7 d, while after 14
d all strains grow and sporulate. Cardinal temperatures: No growth
at 4 and 10 °C, optimum 25 °C (30–32 mm diam), maximum 30 °C
(2–15 mm diam), no growth at 37 °C. Differential parameters: No
growth at 10 °C, oval conidia, large amounts of sterile mycelium
(from Zalar et al. 2007).
Substrates and distribution: Saprobic on fruit surfaces, hypersaline
waters in (sub)tropical climates; Asia (Iran), Central America
(Dominican Republic).
Additional specimens examined: Dominican Republic, isol. from hypersaline water,
salterns (EXF-696, EXF-720, EXF-727); salt lake Enriquillo, isol. from hypersaline
water (EXF-718). Iran, isol. from Citrus fruit (orange) (CPC 11683).
Notes: Cultures of C. dominicanum sporulate less abundantly than
C. sphaerospermum and C. halotolerans and tend to loose their
ability to sporulate with subculturing (Zalar et al. 2007).
46. Cladosporium dracaenatum Thüm., Mycoth. Univ.,
Cent. XIX, No. 1869. 1881. Figs 98–100.
Lectotype (designated here): USA, South Carolina, Aiken, on
living leaves of Cordyline fruticosa (= C. terminalis, Dracaena
cooperi) (Asparagaceae), 1876, H.W. Ravenel, Thümen, Mycoth.
Univ. 1869 (B 700006248). Isolectotypes: e.g., BPI 426454, BPI
426455, BR-MYC 8172,86, HAL, HBG, M-0057504.
Lit.: Saccardo (1892: 605), Schubert (2005b: 67–70).
Ill.: Schubert (2005b: 68–69, igs 22–23, pl. 11, igs A–G).
Exs.: Thümen, Mycoth. Univ. 1869.
110
Fig. 98. Cladosporium dracaenatum (B 700006248). Fascicle of conidiophores and
conidia in vivo. Scale bar = 10 µm. K. Bensch del.
In vivo: Lesions amphigenous, forming distinct patches, irregular in
shape and size, pale to dark grey-brown or dark olivaceous-brown,
without margin, sometimes conluent. Colonies amphigenous,
scattered, in small tufts, caespitose, loose to dense, sometimes
loccose, brown. Mycelium internal, intraepidermal, later also
external, growing supericially; hyphae creeping, branched, 3–9
μm wide, septate, often constricted at the septa and then cells
more or less irregularly swollen, subhyaline, pale olivaceousgreen to pale brown, smooth, walls only slightly thickened, forming
stromata and loose to dense hyphal aggregations. Stromata
small to moderately large, usually well-developed, substomatal to
intraepidermal, subglobose, 15–70 μm wide, few to several layers
deep, composed of swollen hyphal cells, subglobose to somewhat
angular or irregular, pale to medium brown, smooth, walls
thickened. Conidiophores in small to moderately large fascicles,
few to numerous, loosely arranged, arising from stromata, emerging
through stomata or erumpent through the cuticle, or solitary arising
from external hyphae or secondary conidia, erect, straight to more
or less lexuous, often once to several times mildly to distinctly
geniculate-sinuous, sometimes very slightly nodulose, unbranched
or branched, once or twice, 20–240 × 3–5(–5.5) μm, pluriseptate,
septa often not very conspicuous, pale brown, somewhat darker
near the base, pale medium brown, smooth or minutely asperulate
or somewhat irregularly rough-walled with age, sometimes minutely
verruculose near the apex, walls slightly thickened, one-layered,
often slightly swollen at the base. Conidiogenous cells integrated,
terminal and intercalary, cylindrical to often geniculate, 8–40 μm
long, proliferation sympodial, with a single or few conidiogenous
loci, often situated on small lateral shoulders or unilateral swellings,
protuberant, often subdenticulate, short cylindrical, 1–2(–2.5) μm
diam, dome often somewhat higher than the surrounding rim,
thickened and somewhat darkened-refractive. Conidia catenate,
the genuS Cladosporium
apex and base rounded, sometimes slightly attenuated, with age
conidia becoming longer and wider, distinctly swollen, up to 36
μm long or even longer and up to 11 μm wide, with up to seven
septa, septa occasionally somewhat sinuous, darker, pale medium
to medium brown or medium olivaceous-brown, thick-walled with a
somewhat irregular surface ornamentation, sometimes with a single
longitudinal septum, confusable with supericially growing swollen
hyphae, hila protuberant, short cylindrical, truncate, 1–2(–2.5) μm
diam, thickened and somewhat darkened-refractive; microcyclic
conidiogenesis often occurring.
Substrates and distribution: On Cordyline and Dracaena spp.
(Asparagaceae), Ruscus (Asparagaceae); North America –
Cordyline fruticosa (USA), C. indivisa (USA), Cordyline sp. (USA),
Dracaena sp. (USA), Ruscus aculeatus (USA).
Additional specimen examined: USA, California, Berkeley, Euclide Ave. & Regal
Bldg., on Ruscus aculeatus, 17 Oct. 1942, L. Bonar (F 1167647).
Fig. 99. Cladosporium dracaenatum (M-0057504). Conidiophores, old, swollen
conidia and microcyclic conidiogenesis in vivo. Scale bar = 10 µm. K. Bensch del.
Notes: Vanev & Taseva (1990) reported C. dracaenatum on Dracaena
draco from Bulgaria. Baka & Krzywinski (1996) examined fungi
associated with leaf spots of the endemic and now nearly extinct
Dracaena ombet in Sudan and reported C. dracaenatum as one of
the most common isolates and an important reason for the decline
of this tree. They discussed the explosive spread of the disease
either as pointing to an epidemic outbreak of a lethal pathogen
inluenced by external factors, or as a result of long-distance mass
transport. Besides pathogenicity tests, SEM and TEM examination
were carried out to detect and conirm the compatibility between
Dracaena leaves and the pathogen. Remarkable ultrastructural
changes of cell organelles after infection were noticed indicating
that the fungus examined was a true pathogen. Baka & Krzywinski
(1996) did not provide a detailed description of its morphology, but
described branched conidiophores emerging through stomata and
producing new conidia, so it is possible that they actually dealt with
C. dracaenatum. Probably, C. dracaenatum is not conined to North
America and more common than indicated under substrates and
distribution.
The leaf-spotting fungus on Ruscus aculeatus from California is
morphologically indistinguishable from C. dracaenatum.
47. Cladosporium echinulatum (Berk.) G.A. de Vries,
Contr. Knowl. Genus Cladosporium: 49. 1952. Figs 101, 102.
Basionym: Helminthosporium echinulatum Berk., Gard. Chron.
1870: 382. 1870.
≡ Heterosporium echinulatum (Berk.) Berk. & Broome, in Cooke,
Grevillea 5(35): 123. 1877.
Lectotype (selected by David 1997): Great Britain, on Dianthus
caryophyllus (Caryophyllaceae), Feb. 1870, ex herb. M.J. Berkeley
(K).
Fig. 100. Cladosporium dracaenatum (B 700006248 and M-0057504). A.
Symptoms. B. Fascicle of conidiophores. C. Conidiophore and conidia. D. Small
catenate conidia showing surface ornamentation. E. Old swollen conidia forming
secondary conidiophores (microcyclic conidiogenesis). F, G. Conidiophores and old
swollen conidia. Scale bars = 5 (D), 10 (C, E–G), 20 (B) µm.
in branched chains, straight, obovoid, ellipsoid to subcylindrical
or cylindrical, 3.5–26 × 3–7(–8) μm, 0–3-septate, sometimes
slightly constricted at the septa, pale brown, smooth or almost so
to minutely or distinctly verruculose, walls only slightly thickened,
www.studiesinmycology.org
= Heterosporium circinale Klotzsch, Herb. Viv. Mycol., No. 188. 1832, nom.
inval. [syntypes: e.g. B, HAL, M].
= Helminthosporium exasperatum Berk. & Broome, Ann. Mag. Nat. Hist., Ser.
4, 11: 345. 1873 [lectotype: K].
≡ Heterosporium exasperatum (Berk. & Broome) Cooke, Grevillea
16(80): 109. 1888.
= Heterosporium dianthi Sacc. & Roum., Rev. Mycol. (Toulouse) 3(11): 57.
1881 [lectotype: PAD].
= Mycosphaerella dianthi C.C. Burt, Trans. Brit. Mycol. Soc. 20: 214. 1936.
≡ Davidiella dianthi (C.C. Burt) Crous & U. Braun, Mycol. Progr. 2(1): 10.
2003.
= Heterosporium echinulatum var. dianthi Losa, Collect. Bot. (Barcelona) 3:
111
BenSch et al.
Fig. 101. Cladosporium echinulatum (M-0057503, isotype of Heterosporium
circinale). Fascicle of conidiophores and conidia in vivo. Scale bar = 10 µm. K.
Bensch del.
149. 1952 [holotype: BCF].
= ? Heterosporium dianthi Sawada, Bull. Gov. Forest Exp. Sta. 105: 98. 1958,
nom. illeg., non H. dianthi Sacc. & Roum., 1881.
Lit.: Saccardo (1886: 481), Lindau (1910: 81–82), Ellis (1971: 311),
Subramanian (1971: 291–293), Sivanesan (1984: 222), David
(1988b, 1997: 34–40), Ho et al. (1999: 127), Zhang et al. (2003:
90–92).
Ill.: Lindau (1910: 82, ig.), Ellis (1971: 311, ig. 215 A), Subramanian
(1971: 292, ig. 226), Sivanesan (1984: 223, ig. 119), David (1988b:
ig.; 1997: 37–38, igs 7, 8 A–C), IMI Distribution Maps of Plant
Diseases 593 (1988), Ho et al. (1999: 126, igs 15–16), Zhang et
al. (2003: 91, ig. 54).
Exs.: Baenitz, Herb. Eur. 6728; Briosi & Cavara, Fung. Paras.
Piante Colt. Utili Exs. 440; Kabát & Bubák, Fungi Imperf. Exs.
49; Klotzsch, Herb. Viv. Mycol. 188; Migula, Krypt. Germ., Austr.
Helv. Exs. [Pilze] 69; Rabenhorst, Fungi Eur. Exs. 2884; Saccardo,
Mycoth. Ven. 1043; Săvulescu, Herb. Mycol. Roman. 444; Sydow,
Mycoth. March. 2565; Vestergren, Micromyc. Rar. Sel. Praec.
Scand. 998; Vill, Fungi Bav. 995.
In vivo: On living leaves, stems and inlorescences especially
the calyx, leaf spots amphigenous, at irst more or less circular
to ellipsoid, 10–20 mm diam, later becoming conluent, grey to
olivaceous-green, surrounded by a somewhat darker greyish
brown, purple or reddish margin, margin occasionally raised.
112
Colonies amphigenous, scattered to subeffuse, caespitose,
brown, velvety, often formed in the centre of the spots. Mycelium
internal, subcuticular to sometimes intraepidermal; hyphae
sparingly branched, 3–12 μm wide, septate, not constricted or
only slightly so, subhyaline to olivaceous-brown, smooth, walls
only slightly thickened; aerial hyphae only present in culture
(according to David 1997). Stromata usually well-developed,
compact, pseudoparenchymatous, mostly substomatal, sometimes
intraepidermal, small, consisting of only a few swollen hyphal cells,
sometimes larger, a few layers deep, swollen hyphal cells 8–17
μm diam, olivaceous-brown, smooth, thick-walled. Conidiophores
in small loose fascicles, arising from stromata or swollen hyphal
cells, usually emerging through stomata or sometimes erumpent
through the cuticle, erect, substraight to more or less lexuous,
several times distinctly geniculate-sinuous, torulose, subnodulose
to nodulose, unbranched to occasionally branched, formed as short
lateral branches, 40–200(–250) × 7–10(–12) μm, swellings up to
12–15 μm wide, septate, not or only slightly constricted at septa,
pale brown, mostly medium brown, somewhat paler towards the
apex, smooth to distinctly verruculose, walls thickened, sometimes
appearing two-layered, about 1 μm thick, often swollen at the
base, occasionally enteroblastically proliferating. Conidiogenous
cells integrated, terminal and intercalary, several times geniculatesinuous, nodulose, proliferation sympodial with conidiogenous
loci conined to swellings, usually with 1–2 conidiogenous loci
per cell, protuberant, coronate, about 3 μm wide, thickened,
somewhat darkened-refractive. Conidia solitary or in short,
unbranched chains, straight to slightly curved, broadly ellipsoid
to cylindrical, sometimes soleiform with a swollen bulbous basal
cell, (15–)25–55(–65) × (10–)12–15(–17) μm, (0–)1–4(–6)-septate,
sometimes constricted at septa, septa becoming sinuous with age,
pale to medium brown, coarsely verrucose to usually echinulate,
projections up to 1 μm long, walls thickened, up to 1.5(–2) μm thick
(including ornamentation), both ends obtuse, conidial hila raised
in young conidia, in older conidia becoming incorporated in the
conidial wall, 2.5–3.5(–4) μm wide, thickened, darkened-refractive;
microcyclic conidiogenesis occurring.
In vitro (description based on MEA, sparingly fruiting on
SNA): Mycelium branched, 3–8 μm wide, with swellings and
constrictions, therefore sometimes irregular in outline, swellings
up to 16 μm, single hyphal cells sometimes distinctly swollen,
up to 21 μm wide, subhyaline to pale olivaceous, at the base
of conidiophores somewhat darker, smooth, walls unthickened
or slightly thickened. Conidiophores macronematous, arising
mainly terminally from ascending hyphae, sometimes also
laterally from plagiotropous hyphae, erect, straight to usually
lexuous, cylindrical-oblong, once or several times slightly to
distinctly geniculate due to sympodial proliferation, subnodulose
with unilateral shoulders or nodulose with multilateral swellings,
swellings up to 10 μm diam, unbranched, occasionally branched
towards the apex, sometimes proliferating at the apex without
forming additional loci appearing as hyphal apices, up to 730 μm
long or even longer, 5–6 μm wide, pluriseptate, not constricted at
septa, pale to medium olivaceous-brown, sometimes even dark
olivaceous-brown, smooth, walls only slightly thickened (about
0.5 μm wide). Conidiogenous cells integrated, terminal and
intercalary, cylindrical-oblong, once or twice slightly to distinctly
geniculate, subnodulose with loci being situated on unilaterally
swollen shoulders, length variable, with 1–5 loci per cell,
sometimes verruculose, conidiogenous loci conspicuous, usually
protuberant, 2–3 μm wide, about 1 μm high, thickened and
the genuS Cladosporium
Fig. 102. Cladosporium echinulatum (CBS 123191). A–G. Conidiophores and conidial chains in vitro. Scale bars = 10 µm.
darkened-refractive. Ramoconidia occasionally formed. Conidia
solitary or in short unbranched, rarely branched chains, straight,
smaller conidia broadly ovoid, ellipsoid, sometimes obclavate,
8–17 × 5–8(–9) μm [av. (± SD) 13.4 (± 3.3) × 6.5 (± 1.1)],
0–1-septate, larger conidia broadly ellipsoid to subcylindrical,
with age becoming soleiform, lower cell somewhat bulbous,
18–42 × 7–9.5(–10.5) μm [av. (± SD) 28.0 (± 6.9) × 7.9 (± 1.0)],
0–3-septate, septa not very conspicuous, becoming sinuous with
age, pale to medium olivaceous-brown, verruculose to usually
densely echinulate, due to surface ornamentation walls appear to
be very thick-walled, up to 1.5 μm wide, attenuated towards apex
and base, hila conspicuous, (1.5–)2–3 μm diam, thickened and
darkened-refractive; conidia sometimes germinating; microcyclic
conidiogenesis occasionally occurring.
Culture characteristics: Colonies on PDA attaining 24–29 mm after
14 d, pale olivaceous-grey to olivaceous-grey, grey-olivaceous
towards margins, olivaceous-black reverse, loccose, margins
white, glabrous to feathery, narrow, crenate to rarely imbricate,
aerial mycelium loccose, low, loose to dense, growth lat with
somewhat elevated colony centre, without prominent exudates,
sporulating. Colonies on MEA reaching 21–30 mm, olivaceous-grey
to grey-olivaceous, whitish due to aerial mycelium, olivaceous-grey
reverse, velvety to luffy-loccose, margins narrow, white, glabrous
to feathery, somewhat crenate, aerial mycelium luffy-loccose,
loose to dense, white or smoke-grey, growth lat with somewhat
elevated colony centre, radially furrowed and wrinkled, without
prominent exudates, sporulation profuse. Colonies on OA reaching
26–34 mm, grey-olivaceous, whitish due to luffy-loccose aerial
mycelium, leaden-grey or pale greenish-grey reverse, margin
white, regular to somewhat crenate, aerial mycelium abundant,
loose to dense, growth lat, sporulating.
www.studiesinmycology.org
Substrates and distribution: On Dianthus spp. (Caryophyllaceae);
Africa, Asia, Europe, Oceania, North, Central and South America,
widely distributed – Dianthus barbatus (Belgium, Czech Republic,
Denmark, Switzerland, UK), D. caryophyllus (Austria, Brazil,
Canada, China, Czech Republik, Denmark, Finland, France,
Germany, Italy, Kenya, New Zealand, Portugal, Romania, Turkey,
UK, USA), D. carthusianorum (Germany), D. chinensis (China), D.
deltoides (Estonia), D. plumarius (Germany), D. seguieri (Spain),
D. superbus (Taiwan), Dianthus spp. (Argentina, Austria, Belgium,
Brazil, Bulgaria, Canada, Chile, Columbia, Cyprus, Denmark, El
Salvador, Estonia, France, Germany, Hungary, Iceland, India, Iran,
Israel, Italy, Japan, Kazakhstan, Kyrgystan, Lithuania, Mexico,
Morocco, the Netherlands, Norway, Peru, Poland, Slovenia,
South Africa, Spain, Sweden, Switzerland, Uganda, UK, Ukraine,
Uzbekistan, former Yugoslavia).
Additional specimens examined: Czech Republic, Böhmen, near Turnau, on Dianthus
caryophyllus, 29 Sep. 1902, J.E. Kabát, Kabát & Bubák, Fungi Imperf. Exs. 49
(HBG). France, La Varenne near Paris, on Dianthus sp., 1903, P. Hariot, Vestergren,
Micromyc. Rar. Sel. Praec. Scand. 998 (M); Seine-et-Marne, Fontainebleau, on
D. caryophyllus, Mar. 1894, Feuilleaubois, Société du Sud-Est 1894 No. 445 (M).
Germany, Bavaria, Freising, on D. caryophyllus, Nov. 1877, Prof. J.E. Weiss, No.
69 (M); Neustift, in a garden, 3 Oct. 1897, Allescher (M); Schönow, 1897, Prof. J.E.
Weiss (M); Oberfranken, Bamberg, on D. carthusianorum, without date, A. Vill (HBG);
Unterfranken, Gerolzhofen, Rüdenhausen, in the castle garden, on Dianthus sp., Aug.
1907, A. Vill, Vill, Fungi Bav. 995 (M); Berlin, on D. caryophyllus, 1899, Dr. O. Appel (M);
Berlin, garden, on Dianthus plumarius, 1992, P. Magnus (HBG); Brandenburg, Steglitz,
near Berlin, on D. caryophyllus, Mar. 1890, P. Magnus (M); Tamsel, 20 Oct. 1909, P.
Vogel, Flora marchica (M). Israel, Jerusalem, on Dianthus sp., 20 May 1936, Dr. T.
Rayss, Flora Cryptogamica Palaestinae (M). Italy, Torino, on D. caryophyllus, Prof.
P. Voglino, Cavara & Pollacci, Fung. Paras. Piante Colt. Utili Exs. 440 (M). Romania,
Muntenia, distr. Ilfov, Bucureşti, on D. caryophyllus, 15 Aug. 1932, T. Săvulescu & C.
Sandu, Săvulescu, Herb. Mycol. Roman. 444 (M); on Dianthus sp., 8 Oct. 1959 (M).
Slovenia, “Krain, near Zicklach”, on Silene dioica, 14 Mar. 1888, W. Voss (HBG). South
Africa, Natal, on Dianthus sp., Nov. 1910, C. Fuller (HBG). New Zealand, Auckland,
Old Government House, Auckland University Campus, on Dianthus barbatus 20 Apr.
2008, C.F. Hill (CPC 15386 = CBS 123191, reference strain).
113
BenSch et al.
Notes: This species has also been recorded from other members
of the Caryophyllaceae, including e.g., Cerastium, Lychnis,
Saponaria, Silene and Viscaria. Collections on these hosts have
not been seen and are not conirmed, except for a sample on
Silene dioica (Slovenia, “Krain, near Zicklach”, W. Voss, 14 Mar.
1888, HBG), which could be conirmed.
subglobose, ellipsoid, obovoid, fusiform, subcylindrical, 4–24(–26)
× 3–7(–8) μm, 0–3(–5)-septate, usually not constricted at the septa,
subhyaline to pale brown, almost smooth to faintly rough-walled,
walls slightly to conspicuously thickened, apex rounded, with up to
4 hila, base truncate to convex, occasionally somewhat attenuated,
hila protuberant, thickened, darkened, 1–2 μm diam; microcyclic
conidiogenesis occurring.
48. Cladosporium epichloës Lobik, Bolezni Rast. 17(3–4):
189. 1928. Fig. 103.
Substrates and distribution: On Epichloë typhina (Clavitipitaceae);
Europe – E. typhina on Bromus inermis (Russia), E. typhina on
Dactylis polygama (Germany), E. typhina on Holcus lanatus
(Denmark), E. typhina (Austria).
Holotype: Russia, “Bezirk Piatigorsk, im Walde am Abhange
des Berges Maschuk, bei der Lermontowschen Haltestelle”, on
stromata of Epichloë typhina (Clavicipitaceae) on Bromus inermis
(Poaceae), 26 Sep. 1923 (LE-40522).
Lit.: Heuchert et al. (2005: 18–19).
Ill.: Lobik (1928: pl. 8, ig. 86), Heuchert et al. (2005: 18, ig. 2).
Exs.: Keissler, Krypt. Exs. 3392.
In vivo: Colonies olivaceous to dark brown, effuse, diffuse to
dense, conluent, velvety. Mycelium immersed and external;
supericial hyphae creeping, branched, 2.5–6 μm wide, septate,
often constricted at the septa, cells irregularly swollen, up to 8 μm
wide, subhyaline to pale brown, smooth, walls slightly thickened.
Stromata lacking. Conidiophores solitary or in loose groups, arising
from hyphae or swollen hyphal cells, erect to decumbent, straight
or often curved, subcylindrical, geniculate-sinuous, unbranched or
branched, (9–)34–105 × 3–6 μm, slightly swollen at the base, up to 7
μm, 0–7-septate, not constricted at the septa, pale to medium brown,
paler towards the apex, smooth, walls slightly thickened, rarely
with subnodulose intercalary swellings, up to 7 μm wide, swellings
mostly with conidiogenous loci. Conidiogenous cells integrated,
terminal and intercalary, 10–44 μm long, polyblastic, proliferation
sympodial, with (1–)2–9(–11) conspicuous conidiogenous loci
which are sometimes situated on small unilateral swellings
(“shoulders”) protuberant, thickened, darkened-refractive, 1.5–2 μm
diam. Conidia mainly in branched chains, straight, polymorphous,
Additional specimens examined: Austria, Mauerbach near Vienna, on Epichloë
typhina, Jul., C. Keissler, Keissler, Krypt. Exs. 3392 (M-0057568). Denmark, Rømø,
Lakolk, on E. typhina on Holcus lanatus, 24 Jul. 1901, O. Jaap (HBG). Germany,
Sachsen-Anhalt, Harz, Sangerhausen, NNW Grillenberg, “N Kohlenstraße”,
deciduous forest, alt. ca. 379, on E. typhina on Dactylis polygama, 9 Nov. 2003, H.
Jage (herb. Jage, No. 2967/03, HAL 1822).
Notes: This species, conined to Epichloë typhina, is morphologically
close to the common, widespread saprobic species C. herbarum,
which differs, however, in having characteristic intercalary nodes of
7–9 μm diam, such nodes encompassing the entire circumference
of the conidiophore and not just swelling on one side of the
conidiophore as in “shoulders”. The conidiophores of C. epichloës
are often geniculate, with small unilateral shoulders, but multilateral
nodes are lacking. Cladosporium epichloës is morphologically
barely distinguished from C. aecidiicola. Since the latter species
is ecologically clearly discriminated from C. epichloës by being
conined to aecia of rust fungi, we tentatively prefer to maintain
two different species. Inoculation experiments and molecular
examinations are necessary to adequately deine the taxonomy of
the two species and their relations.
49. Cladosporium epimyces Cooke, Grevillea 12(61): 31.
1883. Figs 104–106.
Lectotype (designated by Heuchert et al. 2005): USA, South
Carolina, Aiken, on a gill of Armillaria mellea [≡ Agaricus melleus]
(Agaricaceae), Ravenel & Cooke, Fungi Amer. Exs. 596 (NY).
Isolectotypes: Ravenel & Cooke, Fungi Amer. Exs. 596 (e.g., BPI
426471).
Lit.: Heuchert et al. (2005: 19–22).
Ill.: Heuchert et al. (2005: 20–21, igs 3–4).
Exs.: Ravenel & Cooke, Fungi Amer. Exs. 596.
Fig. 103. Cladosporium epichloës (LE-40522 and HAL 1822). Conidiophores and
conidia in vivo. Scale bar = 10 µm. B. Heuchert del.
114
In vivo: Colonies light to dark brown, effuse, caespitose, velvety,
erect structures delicate, thin. Mycelium immersed and supericial,
hyphae branched, 4–6 μm wide, cells 7–14 μm long, with
constrictions at the septa, pale olivaceous-brown, occasionally
subhyaline, smooth, walls somewhat thickened. Stromata diffuse,
composed of subglobose to rectangular cells, 4–14 μm diam,
subhyaline to pale brown, smooth, walls somewhat thickened.
Conidiophores in loose to dense fascicles, arising from stromata or
formed singly, arising from repent hyphae, erect, sometimes curved,
subcylindrical to geniculate-sinuous, unbranched to branched,
several times, 23–250(–280) × 3–5.5(–7) μm, sometimes swollen
at the very base, up to 9 μm, rarely with some slight intercalary
swellings, up to 7 μm, 0–8(–12)-septate, not or only slightly
constricted at the septa, pale brown, at the base pigmentation
the genuS Cladosporium
Fig. 104. Cladosporium epimyces (NY, lectotype). A. Overview showing loosely
arranged conidiophores and conidia in vivo. B. Diffuse stromata, conidiophores and
conidia in vivo. Scale bars = 10 (B), 50 (A) µm.
Fig. 106. Cladosporium epimyces (PH). Branched conidiophores, ramoconidia,
conidia and microcyclic conidiogenesis. Scale bar = 10 µm. B. Heuchert del.
Fig. 105. Cladosporium epimyces (NY). Conidiophores arising from stromata,
conidia and microcyclic conidiogenesis in vivo. Scale bar = 10 µm. B. Heuchert del.
stronger, paler towards the apex, smooth or almost so, young
conidiophores and conidiophores with attached conidia often
irregularly rough-walled, walls slightly thickened, tips and ultimate
branchlets often unthickened. Conidiogenous cells integrated,
terminal and intercalary, 9–52 μm long, polyblastic, with up to six
conidiogenous loci, sympodially proliferating, conidiogenous loci
somewhat protuberant, thickened, darkened-refractive, 1.5–3 μm
diam. Ramoconidia straight, subcylindrical, 33–53 × 4.5–7 μm,
0–6-septate, occasionally constricted at the septa, basal hilum
slightly convex, without convex dome and surrounding raised
rim, 2–3 μm wide. Conidia catenate, usually in branched chains,
straight to slightly curved, ellipsoid, fusiform, subcylindrical, 4–36(–
44) × 4–7(–8) μm, 0–3-septate, sometimes constricted at the septa,
subhyaline to pale brown, smooth or almost so, young conidia still
attached to the conidiophores sometimes irregularly rough-walled,
walls slightly thickened, sometimes thin-walled, apex with up to
four hila, base truncate to convex, occasionally attenuated towards
the base, hila protuberant, thickened, darkened-refractive, 1–2 μm
diam; microcyclic conidiogenesis seen.
Substrates and distribution: On gill fungi (Agaricales); Europe,
North America – Armillaria mellea (USA), Hypholoma sublatentum
(USA), Lactarius sp. (Poland), Leucopaxillus gentianeus (USA).
www.studiesinmycology.org
Additional specimens examined: Poland, Prószków, Arboret d. Pom. Institutes, on
Lactarius sp., Sep. 1901, (HBG). USA, Pennsylvania, on Hypholoma sublatentum,
2 Aug. 1953, G.T. Reese (PH); Washington, Spokane County, [Pseudotsuga taxifolia
Association, Douglas Fir Zone, Dense near-climax forest, bottom of east facing
slope and adjacent level, 8.2. miles north of N.P. Ry, overpass north of spangle
on west side of U.S. 195, 2300 feet, T. 24N, R. 43E., S. 17.], on Leucopaxillus
gentianeus (= Leucopaxillus amarus), 10 Feb. 1948, W.B. & V.G. Cooke (NY).
Notes: Cooke (1883) described lanceolate, aseptate, hyaline
conidia, 15–20 × 4 μm. The examination of type material and
additional collections showed that the original description of C.
epimyces is very poor and that this species is much more variable.
Aarnaes (2000) recorded this species from Norway, but without any
details about hosts.
50. Cladosporium episclerotiale Bubák, Ann. Mycol. 12:
213. 1914. Fig. 107.
Holotype: Italy, Tyrol, Arco, on sclerotia of Monilinia laxa
(= Sclerotinia cinerea) (Sclerotiniaceae) on mummiied fruits of
Prunus domestica (Rosaceae), Mar. 1913, E. Diettrich-Kalkhoff
(BPI 426531).
Lit.: Saccardo (1931: 797), Heuchert et al. (2005: 22–24).
Ill.: Heuchert et al. (2005: 23, ig. 5).
Exs.: Jaap, Fungi Sel. Exs. 175; Sydow, Mycoth. Germ. 1781.
115
BenSch et al.
shoulders, formed by sympodial geniculation, truncate, 1–2(–3) μm
diam, convex dome and periclinal rim often not very conspicuous
(light microscopy), thickened, darkened-refractive. Conidia usually
in branched chains, straight, subglobose, ellipsoid, broadly ellipsoid,
6.5–23(–30) × 4–8 μm, 0–4-septate, often slightly constricted at
the septa, cells often distinctly rounded, septa often thickened and
darkened, occasionally with distosepta, olivaceous to dark brown,
smooth to irregularly verruculose, walls thickened, mostly distinctly
two-layered, apex rounded or truncate to convex, with up to four
hila, base truncate to convex, hila mostly somewhat protuberant,
1–2.5(–3) μm wide and up to 1.5 μm high, thickened and mostly
distinctly darkened, occasionally not darkened, i.e., hila paler
than the surrounding conidial base; microcyclic conidiogenesis
occasionally occurring.
Substrates and distribution: On Sclerotinia cinerea (Sclerotiniaceae)
and cap and gill fungi; Europe – on Prunus domestica (Germany,
Italy), Prunus spinosa (Germany), Suillus bovinus (Germany), gill
of a mushroom (Germany).
Additional specimens examined: Germany, Bavaria, Gerolzhofen, on dried fruits
of Prunus spinosa, Apr. 1912, A. Vill (HBG, as C. herbarum); Brandenburg, Kreis
Nieder-Barnim, forest between Sophienstädt and Ruhlsdorf, on decaying gills of
Boletus bovinus (= Suillus bovinus), 7 Nov. 1917, P. Sydow, Sydow, Mycoth. Germ.
1781 (M-0057567, HBG, as C. fuligineum) [mixed collection with C. herbarum];
Kreis Prignitz, Triglitz, on a dead gill of a mushroom in a pine forest, 4 Oct. 1905, O.
Jaap, Jaap, Fungi Sel. Exs. 175 (B 700006384, as C. fuligineum Bonord.);
Fig. 107. Cladosporium episclerotiale (BPI 426531). Conidiophores and conidia in
vivo. Scale bar = 10 µm. B. Heuchert del.
In vivo: Colonies blackish brown, diffuse to dense, conluent,
caespitose, velvety. Mycelium immersed and external, supericial;
hyphae 3.5–7 μm wide, closely septate (with eu- and pseudosepta),
often constricted at the septa, hyphal cells irregularly swollen,
medium olivaceous-brown, smooth or almost so, walls thickened,
conspicuously two-layered, outer layer slightly pigmented to
subhyaline. Stromata diffuse, loose to dense, composed of swollen
hyphal cells, subglobose, 3–10 μm diam, medium olivaceousbrown to dark brown, thick-walled, stromatic and hyphal cells
with oil droplets. Conidiophores solitary, arising from hyphal
cells, aggregations or from stromata, erect, straight to curved,
subcylindrical, more or less geniculate-sinuous, unbranched or
branched, (22–)97–295(–322) × 5–8(–10) μm, enlarged at the
base, sometimes conspicuously swollen, 8–13 μm wide, somewhat
attenuated towards the apex, 3–21-septate (with eusepta as
well as pseudosepta), cells 10–20 μm long, olivaceous-brown to
medium brown, pigmentation often stronger near the base, paler
towards the apex, smooth or almost so, walls thickened below, less
thickened towards the apex, wall near the tip often unthickened,
occasionally with slightly intercalary swellings, up to 10 μm diam,
often with conidiogenous loci, wall composed of two distinct layers,
outer layer paler, often with an irregular surface, not constricted
at the septa, conidiophores occasionally with oil droplets.
Conidiogenous cells integrated, terminal and intercalary, 10–55
μm long, polyblastic, with 1–7 conidiogenous loci, proliferation
sympodial, loci non- to slightly protuberant, sometimes on small
116
Notes: The generic afinity of this species, based on the structure
of the conidiogenous loci, was not clearly discernable by means of
light microscopy since the central dome and periclinal raised rim are
often not very distinct. However, it could clearly be demonstrated
by SEM that C. episclerotiale belongs in Cladosporium s. str.
(Heuchert et al. 2005).
The substrates of two of the examined collections are not quite
certain. The collection “Jaap, Fungi Sel. Exs. 175” (B 700006384),
on a dry gill of a mushroom, is totally overgrown with C. herbarum,
but a few blackish brown, caespitose, effuse colonies proved to
be morphologically indistinguishable from C. episclerotiale. In
a collection on dried fruits of Prunus spinosa (HBG), it was not
possible to ascertain if sclerotia were present.
51. Cladosporium eriobotryae Pass. & Beltrani, Transunti,
Ser. 3, 7(1): 38. 1882. Fig. 108.
Holotype: Italy, Sicily, Monostalla, on dry leaves of Eriobotrya
japonica (Rosaceae), Sep. 1878 (not preserved). Neotype
(designated here): Germany, Berlin, Botanical Garden, on
Eriobotrya japonica, 2 May 1899, P. Hennings (B 700006326).
Lit.: Saccardo (1886: 356), Lindau (1907: 824), Ferraris (1912:
344).
In vivo: On living leaves, leaf spots small, vein-limited to large,
irregularly shaped, brown, margin indeinite or with a narrow
darker marginal line. Colonies amphigenous, mainly hypophyllous,
punctiform, scattered to loosely aggregated, dark brown to blackish.
Mycelium internal, occasionally with a few supericial hyphae
arising from swollen hyphal cells. Stromata well-developed, 10–
60(–90) μm diam, substomatal to intraepidermal, brown, composed
of swollen hyphal cells, 3–8 μm diam, walls somewhat thickened.
Conidiophores in small to moderately large fascicles, loose to
the genuS Cladosporium
Fig. 108. Cladosporium eriobotryae (B 700006326). Conidiophores, conidia and
microcyclic conidiogenesis in vivo. Scale bar = 10 µm. U. Braun del.
moderately dense, arising from stromata, emerging through
stomata or erumpent, erect, unbranched, straight, subcylindrical or
attenuated towards the tip to usually distinctly geniculate-sinuous
or subnodulose, 20–120 × 3–8 μm, 1– pluriseptate throughout,
olivaceous to medium brown throughout or paler towards the
apex, smooth, occasionally rough-walled, walls up to 1 μm
wide. Conidiogenous cells integrated, terminal and intercalary,
(5–)10–20(–25) μm long, conidiogenous loci conspicuous,
distinctly coronate, 1–2 μm diam, slightly protuberant, somewhat
darkened-refractive. Conidia catenate, in simple or branched
chains, ellipsoid-ovoid, obovoid, fusiform, subcylindrical, 7–22 ×
(2–)3–7 μm, 0–3(–4)-septate, pale olivaceous to medium brown or
olivaceous-brown, verruculose, thin-walled (≤ 1 μm), ends rounded
to somewhat attenuated, hila 0.75–1.5(–2) μm diam; microcyclic
conidiogenesis occurring.
Substrate and distribution: On Eriobotrya japonica; Europe
(Germany, Italy).
Notes: Type material of this species could not be traced. The
German collection, proposed to serve as neotype, agrees well with
the brief original description. Due to the description of up to 3-septate
conidia, the original type of C. eriobotryae was undoubtedly not
identical with Fusicladium eriobotryae [= F. pomi, see Schubert et
al. 2003]. Cladosporium eriobotryae differs from C. fusicladiiformis,
also described from Eriobotrya japonica, in having much shorter,
non-dimorphic, unbranched conidiophores and darker, verruculose
conidia with up to four septa.
52. Cladosporium exasperatum Bensch, Summerell, Crous
& U. Braun, Stud. Mycol. 67: 40. 2010. Figs 109–111.
Holotype: Australia, Northern Territory, Edith Falls, S 14º05’20’’ E
132º05’12’’, isol. from Eucalyptus tintinnans (Myrtaceae), 23 Sep.
2007, coll. B.A. Summerell, isol. P.W. Crous (CBS H-20431). Extype culture: CBS 125986 = CPC 14638.
Ill.: Bensch et al. (2010: 41−42, igs 26−28).
www.studiesinmycology.org
Fig. 109. Cladosporium exasperatum (CBS 125986). Macro- and micronematous
conidiophores, mycelium and conidial chains in vitro. Scale bar = 10 µm. K. Bensch
del.
In vitro: Mycelium immersed and supericial; hyphae loosely
branched, (1−)1.5−4 μm wide, septate, not constricted at septa,
subhyaline to pale or medium olivaceous-brown, smooth to
verrucose or irregularly rough-walled, walls unthickened or
almost so, sometimes irregular in outline due to swellings,
occasionally swollen at the base of conidiophores, up to 6 μm
wide. Conidiophores macro- and semimacronematous, solitary,
arising laterally or terminally from hyphae, erect, straight to slightly
lexuous, cylindrical-oblong, sometimes once, occasionally slightly
to distinctly twice geniculate-sinuous below the apex or on a lower
level, non-nodulose, unbranched or once branched, 15−100 ×
(2−)2.5−4 μm, septate, not constricted at septa, pale to usually
medium olivaceous-brown, sometimes dark olivaceous-brown,
asperulate or irregularly rough-walled, walls unthickened or only
very slightly thickened, occasionally slightly attenuated towards the
apex. Conidiogenous cells integrated, terminal, cylindrical-oblong,
11−40 μm long, sometimes once, rarely twice geniculate-sinuous,
with loci situated on small lateral shoulders, with a single or up to
3(−4) apical loci, conspicuous, subdenticulate to denticulate, 1−1.5
μm diam, periclinal rim and central dome clearly visible, somewhat
thickened and darkened-refractive. Ramoconidia cylindricaloblong, 19−40 × 2.8−4 μm, 0−2-septate, concolorous with tips of
conidiophores, smooth, base not cladosporioid, broadly truncate,
2.8−4 μm wide, unthickened and not darkened. Conidia catenate,
in branched chains, 2−6 conidia in the unbranched terminal part of
the chain, dichotomously branched or branching in all directions,
small terminal conidia obovoid to ellipsoid-ovoid, sometimes
subglobose, 4−9 × 3−4.5 μm (av. ± SD: 6.7 ± 1.6 × 3.9 ± 0.5),
aseptate, apex usually rounded, intercalary conidia ellipsoid-ovoid
to fusiform, 7−15 × 3−4.5 μm (av. ± SD: 10.5 ± 2.6 × 3.9 ± 0.4),
0(−1)-septate, not constricted, with 1−2 distal hila, secondary
ramoconidia ellipsoid, fusiform to subcylindrical, 9.5−30(−37)
× (2.5−)3.5−4.5(−5) μm (av. ± SD: 19.2 ± 6.2 × 3.9 ± 0.6),
117
BenSch et al.
Fig. 110. Cladosporium exasperatum (CBS 125986). A–C, E–H. Macronematous conidiophores and conidial chains. D. Ramoconidium seceding at the tip of a conidiophore.
Scale bar = 10 µm.
Fig. 111. Cladosporium exasperatum (CBS 125986). A. Ornamented conidia on aerial structures. Note the small scars with an ornamentation free zone. B. Secondary
ramoconidium with a scar and aerial structures. C. Scars on a conidiophore. Note the smooth surface of the conidiophores. D. Ornamented secondary ramoconidium on a
conidiophore with visible line of delineation. E. Septate aerial structure with conidiophores, conidia and scars as background. F. Two ornamented globose conidia adhered to an
aerial structure showing an irregularly reticulate surface. G. Segmented differentiated hyphae on the agar surface giving rise to numerous conidiophores, conidiophore initials
and aerial structures. H. Detail of differentiated substrate hyphae. I. Segmented differentiated hyphae in initials. Scale bars = 2 (A), 5 (B–F, H), 10 (G, I) µm.
118
the genuS Cladosporium
0−2-septate, not constricted, sometimes slightly swollen or wider at
septa, septa median, somewhat in the upper half or occasionally in
the lower third, pale olivaceous to often medium or dark olivaceousbrown, slightly to distinctly irregularly verruculose-rugose (LM),
surface with irregularly reticulate structure or embossed stripes
under SEM probably caused by diminishing turgor and shriveling of
tender conidia, walls thickened, occasionally distinctly constricted
in the middle, attenuated towards apex and base, with 1−4 distal
hila, hila conspicuous, subdenticulate to denticulate, 0.5−1.5 μm
diam, more or less thickened and darkened-refractive; microcyclic
conidiogenesis occurring.
Culture characteristics: Colonies on PDA attaining 26−38 mm diam
after 14 d, zonate, centre olivaceous-grey, then grey-olivaceous,
iron-grey and dull green, reverse iron-grey to dull green, loccose to
luffy, margin regular to undulate, white, glabrous to feathery, aerial
mycelium loccose to luffy, loose to dense, covering large areas
of the colony, growth effuse, with elevated colony centre, without
prominent exudates, sporulation profuse. Colonies on MEA attaining
12−15 mm diam after 14 d, surface and reverse olivaceous-grey,
loccose, margins crenate, very narrow, white, glabrous, aerial
mycelium abundant, loccose, dense, covering the whole surface,
growth convex but radially furrowed and wrinkled in colony centre,
no exudates, sporulating. Colonies on OA reaching 39−54 mm diam
after 14 d, olivaceous-grey to grey-olivaceous due to abundant
sporulation, reverse olivaceous-grey to pale greenish grey, velvety
to loccose, margins regular, colourless to white, glabrous, aerial
mycelium abundant, loccose to luffy, loose to dense, covering large
parts of the colony surface, growth lat, without exudates.
Substrate and distribution: On Eucalyptus tintinnans; Australia.
Notes: Cladosporium exasperatum, which formed a distinct lineage
in phylogenetic analyses as sister to C. scabrellum (Bensch et al.
2010, ig. 1, part a), is a member of the C. cladosporioides s.lat.
complex and belongs to a group of species with distinctly roughwalled conidia. It differs from all allied taxa in having a unique
verruculose-rugose surface ornamentation. Furthermore, it is
characterized in vitro by forming very slow growing small colonies
on all media. Cladosporium pini-ponderosae and C. acalyphae
are two comparable species with distinct surface ornamentation,
which are, however, easily distinguishable by having much longer
conidiophores and some differences in the size of the conidiogenous
loci and size and shape of conidia (Bensch et al. 2010).
53. Cladosporium exile Bensch, Glawe, Crous & U. Braun,
Stud. Mycol. 67: 43. 2010. Figs 112–114.
Holotype: USA, Washington, Seattle, University of Washington
campus, isol. from chasmothecia of Phyllactinia guttata
(Erysiphales) on leaves of Corylus avellana (Betulaceae), 12 Feb.
2004, D. Glawe (CBS H-20432). Ex-type culture: CBS 125987
= CPC 11828.
Ill.: Bensch et al. (2010: 43−45, igs 29−31).
In vitro: Mycelium immersed and supericial; hyphae sparingly
branched, 2−4 μm wide, septate, sometimes constricted at
septa, sterile hyphae subhyaline, fertile hyphae pale or medium
olivaceous-brown, smooth to minutely verruculose or irregularly
www.studiesinmycology.org
Fig. 112. Cladosporium exile (CBS 125987). Macro- and micronematous
conidiophores, mycelium sometimes forming ropes and conidial chains in vitro.
Scale bar = 10 µm. K. Bensch del.
rough-walled where conidiophores are formed, walls unthickened
to slightly thickened, sometimes forming ropes or swollen at the
base of conidiophores, up to 7 μm wide. Conidiophores macroand micronematous, solitary, arising terminally and laterally from
hyphae, erect or ascending, straight to lexuous, cylindrical-oblong,
slightly geniculate towards the apex, sometimes subnodulose,
unbranched or sometimes once, occasionally twice branched,
several long conidiophores up to 305 μm, but mostly shorter,
6−100 μm long, (2−)3−4(−5) μm wide, sometimes wider or even
swollen at the base, up to 8 μmwide, pluriseptate, mostly 0−4
septa, long conidiophores with up to nine septa, sometimes slightly
constricted at septa, pale brown to medium olivaceous-brown,
sometimes paler towards the base, smooth or almost so to minutely
verruculose or irregularly roughwalled, walls unthickened or slightly
thickened, about 0.5 μm wide. Micronematous conidiophores
narrower, shorter and paler, with 1−2 apical loci. Conidiogenous
cells integrated, terminal, sometimes intercalary, cylindrical-oblong,
sometimes geniculate-sinuous, occasionally subnodulose, 6−34
μm long, with up to six loci per cell, crowded at or towards the apex,
sometimes loci situated on small lateral shoulders, subdenticulate
to denticulate, (0.7−)1−2 μm diam, thickened and darkenedrefractive. Ramoconidia cylindrical-oblong, 17−41 × 2.5−4(−5) μm,
0−1-septate, a single ramoconidium with four septa, base broadly
truncate, 2.5−3 μm wide, unthickened, somewhat refractive. Conidia
catenate, in branched chains, up to four conidia in the unbranched
terminal part of the chain, small terminal conidia subglobose to
mostly obovoid, 3.5−5(−5.5) × 2−3 μm (av. ± SD: 4.4 ± 0.7 × 2.4 ±
0.4), intercalary conidia ovoid to ellipsoid, (4−)5−8(−9) × (2−)2.5−3
μm (av. ± SD: 6.3 ± 1.2 × 2.9 ± 0.3), aseptate, with 1−2(−3) distal
hila, secondary ramoconidia ellipsoid to subcylindrical or cylindrical,
with up to three apical hila, 7−25(−35) × 2.5−3.5(−4) μm (av. ± SD:
15.6 ± 7.7 × 3.1 ± 0.4), 0−1(−3)-septate, not constricted at septa,
subhyaline to pale olivaceous-brown, almost smooth to asperulate
or minutely verruculose, under SEM surface almost smooth to
reticulate or with embossed stripes caused by diminishing turgor
and shriveling of tender young conidia, walls unthickened to
slightly thickened, slightly attenuated towards apex and base,
hila protuberant, subdenticulate to denticulate, 0.5−2 μm diam,
thickened and darkened-refractive; microcyclic conidiogenesis
sometimes occurring.
119
BenSch et al.
Fig. 113. Cladosporium exile (CBS 125987). A–G. Macronematous conidiophores and conidial chains. Scale bars = 10 µm.
Culture characteristics: Colonies on PDA olivaceous to glaucousgrey, reverse pale olivaceous-grey to olivaceous-grey, powdery
to luffy, margin white, narrow, somewhat feathery and shiny
like metal, aerial mycelium diffuse, loose, luffy, high, growth low
convex, somewhat folded, without exudates, sporulation profuse.
Colonies on MEA olivaceous-grey in the centre, greenish grey and
glaucous-grey towards margins, zonate, reverse olivaceous-grey,
woolly-felty, margin white, narrow, somewhat feathery, radially
furrowed, folded and wrinkled, aerial mycelium low to high, loose
to more dense, luffy, without exudates, sporulating. Colonies on
OA dark smoke-grey to olivaceous-grey and iron-grey, reverse
leadengrey, powdery to woolly-felty, margin narrow, white or
colourless, glabrous, aerial mycelium loose, luffy to felty, growth
lat to low convex, without exudates, sporulating.
Substrate and distribution: Isolated from chasmothecia of
Phyllactinia guttata on leaves of Corylus; USA.
Notes: In phylogenetic analyses, this species formed a separate
lineage as sister to C. paracladosporioides (Bensch et al. 2010,
ig. 1, part a), i.e., it belongs to the C. cladosporioides complex.
The latter species is easily distinguishable by its usually smooth
conidiophores and conidia, and somewhat longer and wider, mainly
aseptate secondary ramoconidia.
120
54. Cladosporium exobasidii Jaap
54.1. Cladosporium exobasidii Jaap, Verh. Bot. Vereins
Prov. Brandenburg 49: 29. 1907 var. exobasidii. Fig. 115.
Lectotype (designated in Heuchert et al. 2005): Germany, Rhön,
Gersfeld, Rotes Moor, on galls of Exobasidium vaccinii var. vaccinii
(Exobasidiales) on Vaccinium uliginosum (Ericaceae), 30 Jul. 1906,
O. Jaap (B 700006339). Isolectotypes: Jaap, Fungi Sel. Exs. 200
(e.g. HAL, M-0057603).
Lit.: Lindau (1907: 808, 1910: 796), Lind (1913: 522), Saccardo
(1913a: 1371), Braun (2001: 57), Heuchert et al. (2005: 24–26).
Ill.: Braun (2001: 55, ig. 3), Heuchert et al. (2005: 25, ig. 6).
Exs.: Jaap, Fungi Sel. Exs. 200.
In vivo: Colonies on galls, olivaceous-brown to dark brown,
occasionally greyish, loosely to densely caespitose, effuse, erect
structures slightly shiny. Mycelium immersed; hyphae branched,
2−9 μm wide, cells 3−14 μm long, often constricted at the septa,
subhyaline to olivaceous-brown, cells smooth, walls unthickened
or only slightly thickened, swollen hyphal cells 3−13 μm diam,
forming loose to dense aggregations. Conidiophores solitary or in
the genuS Cladosporium
Fig. 114. Cladosporium exile
(CBS 125987). A. Conidia
and secondary ramoconidia
with reticulate ornamentation,
near long aerial hyphae or
conidiophores. B. CryoSEM of
different types of conidia on aerial
structures. Note a remarkable
pattern
of
blastoconidium
formation (backwards) (arrow).
C. Numerous hyphae and
conidiophores in this overview
of a colony of the fungus. D.
Hyphae on the agar surface. E.
Conidia and aerial structures.
F. Detail of conidia and scars.
Scale bars = 2 (F), 5 (A–B), 10
(E), 50 (C–D) µm.
loose to dense fascicles, arising from hyphal aggregations, erect to
decumbent, straight to curved, subcylindrical, slightly geniculatesinuous, usually unbranched, rarely branched in the upper third, 25400 × 3.5−6.5(−8) μm, occasionally longer, sometimes somewhat
swollen at the base, 6−11 μm wide, 3−11-septate, not constricted
at the septa, pale to medium olivaceous-brown, paler towards
the apex, tips often subhyaline, smooth, but occasionally roughwalled in the upper third, walls thickened, but often thin-walled near
the tips, occasionally enterogenously proliferating, monopodial,
sometimes with intercalary swellings, 5−9 μm wide, swellings often
with numerous conidiogenous loci, tips often somewhat capitate
and provided with numerous conspicuous loci. Conidiogenous cells
integrated, terminal and intercalary, subcylindrical, 14−108 μm long,
polyblastic, sympodially proliferating, conidiogenous loci crowded,
usually conspicuously protuberant, thickened and darkened,
1.5−2.5 μm wide. Ramoconidia clavate, subcylindrical, 14−29(−30)
× 4−5.5 μm, 0−1(−3)-septate, without any constrictions, base slightly
convex, without dome and raised rim, 2.5−3 μm wide. Conidia
numerous, usually in branched chains, straight, occasionally
slightly curved, subglobose, obovoid, ellipsoid, subcylindrical, 2−19
× 2−6 μm, 0−1(−2)-septate, without any constrictions, subhyaline
to pale olivaceous, smooth, wall unthickened to slightly thickened,
apex rounded, somewhat attenuated to truncate, with up to ive
hila, base truncate, convex or slightly attenuated, hila protuberant,
thickened and darkened, 1−2 μm diam; microcyclic conidiogenesis
occurring.
Substrates and distribution: On Exobasidium spp. (Exobasidiales);
Europe – on E. rhododendri (Austria), E. vaccinii on Andromeda
polifolia (Sweden), E. vaccinii on Vaccinium uliginosum (Denmark,
Germany), E. vaccinii on V. vitis-idaea (Germany), E. vaccinii on
Vaccinium spp. (Poland, Ukraine).
www.studiesinmycology.org
Additional specimens examined: Austria, Kärnten, Oberseeland in the Karawanken,
on Exobasidium rhododendri, 31 Jul. 1907, O. Jaap (HBG). Denmark, Jütland,
Wilborg, on galls of Exobasidium vaccinii var. vaccinii on Vaccinium uliginosum, 14
Jun. 1906, J. Lind (B 700006341). Germany, Bavaria, Mittelfranken, near Girndorf,
on Exobasidium vaccinii on Vaccinium vitis-idaea, 29 Jun. 1898, A. Schwarz (HBG);
“Föhrenwald auf Diluvialsand”, E of Nürnberg, 3 Jul. 1898, A. Schwarz (HBG);
between Schwarzenbach and Unterburg near Nürnberg, 9 Jul. 1907, A. Schwarz
(HBG); Brandenburg, forster’s house Briese near Birkenwerder, 8 Jun. 1901, P.
Röseler (HBG, as C. fuligineum); Kreis Prignitz, Putlitzer Heide bei Putlitz, on galls
of Exobasidium vaccinii var. vaccinii on Vaccinium uliginosum, 1 Sep. 1900, O. Jaap
(B 700006340).
Notes: The collection on Exobasidium vaccinii var. vaccinii on
Vaccinium uliginosum from Denmark (B 700006341) cannot be
considered a paratype as cited by Braun (2001: 57) since this
collection was not cited by O. Jaap in the protologue of the original
description. Another original collection from the herbarium in BerlinDahlem (B 700006339), cited by Jaap, was selected as lectotype (this
sample cannot be considered the holotype since Jaap listed several
original collections, but did note designate any types). Duplicates of
the lectotype, distributed in Jaap’s Fungi Sel. Exs. are isolectotypes.
De Vries (1952) and Ho et al. (1999) cited C. exobasidii as
a synonym of C. cladosporioides, although in the latter species
the conidiophores are characterised by lacking swellings and
geniculations, and the conidiogenous loci are not crowded (Braun
2001). Oudemans (1919) and Lind (1934) regarded C. exobasidii to
be a synonym of C. fuligineum (= C. herbarum), although the latter
species is clearly distinguished by having verruculose to verrucose
conidia. A collection on Exobasidium rhododendri (HBG) has been
examined, but was too old and scarce to be provable.
54.2. Cladosporium exobasidii var. verruculosum
Heuchert, U. Braun & K. Schub., Schlechtendalia 13: 27.
2005. Fig. 116.
121
BenSch et al.
Fig. 116. Cladosporium exobasidii var. verruculosum (HBG). Conidiophores and
conidia in vivo. Scale bar = 10 µm. B. Heuchert del.
Fig. 115. Cladosporium exobasidii var. exobasidii (B 700006339). Conidiophores,
ramoconidia and conidia in vivo. Scale bar = 10 µm. B. Heuchert del.
Holotype: Czech Republic, near Pontresina, on Exobasidium
vaccinii var. vaccinii (Exobasidiales), 3 Sep. 1899, P. Magnus
(HBG).
Ill.: Heuchert et al. (2005: 27, ig. 7).
In vivo: Colonies olivaceous, loose to dense, effuse, caespitose.
Mycelium immersed; hyphae branched, 2−5 μm wide, cells
5−20 μm long, not constricted at the septa or only slightly so,
olivaceous-brown, smooth, walls unthickened. Stromata lacking.
Conidiophores solitary, arising from hyphae, often densely
arranged, erect to decumbent, geniculate-sinuous, mostly
unbranched, occasionally branched in the upper third, 2−217 ×
2−6 μm, slightly enlarged at the base, up to 7 μm, 1−6-septate,
without any constrictions, pale to dark olivaceous-brown, rarely
subhyaline, usually paler towards the apex, smooth, walls
slightly thickened. Conidiogenous cells integrated, terminal
and intercalary, 14−53 μm long, polyblastic, conspicuously
geniculate-sinuous, occasionally slightly widened, conidiogenous
loci protuberant, thickened, darkened, 1−2 μm diam. Conidia
usually in branched chains, rarely slightly curved, broadly
ellipsoid, fusiform, obovoid, 5−23 × 3−7 μm, 0−1−2)-septate,
secondary ramoconidia broadly ellipsoid, subcylindrical, 10−38
× 4−7 μm, 0−2(−3)-septate, at the apex with up to four hila,
without any constrictions, pale olivaceous-brown to medium
brown, verruculose, walls unthickened, apex rounded, truncate
or somewhat attenuated, convex, base truncate to convex, hila
conspicuously protuberant, thickened and darkened, 1−2 μm
diam; microcyclic conidiogenesis not observed.
122
Substrates and distribution: On Exobasidium spp. (Exobasidiales);
Europe – on E. vaccinii var. vaccinii (Czech Republic), on
Exobasidium warmingii (Switzerland).
Additional specimen examined: Switzerland, near Davos, on Exobasidium
warmingii on Saxifraga aizoon, 22 Jul. 1901, Travel (HBG).
Notes: This variety is distinguished from var. exobasidii by having
verruculose conidia. Conidiophores with nodulose swellings and
true ramoconidia are lacking in var. verruculosum, but swellings
are also not consistently formed in var. exobasidii. Otherwise the
two varieties are congruent.
55. Cladosporium ferox (Kabát & Bubák ex Lindau) J.C.
David, Mycol. Pap. 172: 40. 1997. Fig. 117.
Basionym: Heterosporium ferox Kabát & Bubák ex Lindau, Rabenh.
Krypt.-Fl., Ed. 2, 1(9): 83. 1910.
≡ Heterosporium ferox Kabát & Bubák, Fungi Imperf. Exs., Fasc. 9, No.
444. 1907, nom. inval.
Lectotype (selected by David 1997): Czech Republic, Tábor,
botanic garden, on Ranunculus arvensis (Ranunculaceae), 12 Jul.
1906, Kabát & Bubák, Fungi Imperf. Exs. 444 (BPI 802184).
Lit.: Lindau (1910: 83).
Ill.: David (1997: 41–42, igs 9–10).
Exs.: Kabát & Bubák, Fungi Imperf. Exs. 444.
In vivo: Leaf spots amphigenous, small, regular, brown patches.
Mycelium immersed, branched, septate, hyaline, 2.5–7.5 μm wide.
Stromata absent. Conidiophores densely fasciculate, up to 30,
arising from the host surface, erect, sometimes curved, subcylindrical
to geniculate-sinuous, above all in the upper half, unbranched, 90–
150(–200) × 6–8.5 μm, septate, uniformly pale to medium brown,
thin-walled (< 1 μm), smooth, old conidiophores sometimes inely
the genuS Cladosporium
Fig. 118. Cladosporium labelliforme (CBS 126345). Conidiophores and conidia in
long often dichotomously branched chains in vitro. Scale bar = 10 µm. K. Bensch
del.
Fig. 117. Cladosporium ferox (BPI 802184). Conidiophores and conidia in vivo.
Scale bar = 10 µm. U. Braun del.
rough-walled. Conidiogenous cells integrated, terminal, 10–40
μm long, conidiogenous loci conspicuous, somewhat protuberant,
thickened and darkened, broad and lat, 2.5–3.5 μm diam. Conidia
solitary, cylindrical to subclavate, straight, occasionally somewhat
curved, (25–)30–55(–65) × 7–9 μm, (1–)3–4(–5)-septate, not
constricted at the septa, pale olivaceous, thin-walled, smooth,
old conidia sometimes inely rough-walled, apex obtuse, broadly
rounded, somewhat tapering towards the base, basal hilum not or
somewhat protuberant, coronate, with a thickened periclinal rim
which may be inclined inwards, central dome conical, 2 μm diam,
with a distinct central pore.
Substrates and distribution: On Ranunculus arvensis; Czech
Republic.
Notes: Lindau (1910) commented that this species infected aerial
parts of the host plant, but not usually the lowers, gradually causing
its degeneration until it dies.
56. Cladosporium labelliforme Bensch, Summerell, Crous
& U. Braun, Stud. Mycol. 67: 45. 2010. Figs 118, 119.
Holotype: Australia, Northern Territory, Fogg Dam, S 12º34’01’’
E 131º17’49’’ isol. from Melaleuca cajuputi (Myrtaceae), 23 Sep.
2007, coll. B.A. Summerell, isol. P.W. Crous (CBS H-20433). Extype culture: CBS 126345 = CPC 14523.
Ill.: Bensch et al. (2010: 46−47, igs 32−33).
In vitro: Mycelium immersed and supericial; hyphae iliform to
cylindrical-oblong, unbranched or sparingly branched, 0.5−1 μm
wide, at the base of conidiophores wider, 1.5−2.5 μm wide, septate,
not constricted, septa often not very conspicuous, subhyaline to
www.studiesinmycology.org
very pale olivaceous or pale olivaceous-brown, smooth, walls
unthickened, forming dense ropes or iliform hyphae often spirally
twisted. Conidiophores macronematous, solitary, arising terminally
and laterally from hyphae, erect, straight, cylindrical-oblong,
neither nodulose nor geniculate, unbranched, 24−90 × 2−3.5(−4)
μm, 0−3-septate, not constricted at septa, very pale olivaceousbrown or olivaceous, smooth or inely verruculose, asperulate,
walls unthickened and somewhat irregular towards the base.
Conidiogenous cells integrated, terminal, cylindrical-oblong,
neither geniculate nor nodulose, 11−42 μm long, with 1−3 loci at the
apex, conspicuous, subdenticulate, 1−1.5 μm diam, thickened and
darkened-refractive. Ramoconidia occasionally formed, cylindricaloblong, up to 50 μm long, asperulate as tips of conidiophores, not
attenuated towards the base, base about 2.5 μm wide, unthickened
and not darkened. Conidia catenate, in long branched chains, often
dichotomously branched, up to 7(−9) conidia in the unbranched
terminal parts of the chain, conidial chains labellate (spread in a
fan-like manner), small terminal conidia obovoid or ellipsoid, 4.5−8
× 1.5−2.5 μm (av. ± SD: 6.1 ± 1.2 × 2.0 ± 0.3), intercalary conidia
fusiform to ellipsoid or subcylindrical, 7−16(−18) × 1.5−3 μm (av.
± SD: 10.0 ± 3.0 × 2.5 ± 0.5), aseptate, occasionally with a single
septum, attenuated towards apex and base, with 1−2(−3) distal
hila, secondary ramoconidia fusiform to cylindrical-oblong, 11−27 ×
(2−)2.5−3(−3.5) μm (av. ± SD: 19.2 ± 4.9 × 2.8 ± 0.3), aseptate, very
pale olivaceous or olivaceous-brown, smooth, walls unthickened,
with 2−3(−4) distal hila, hila conspicuous, subdenticulate,
0.5−1.5 μm diam, thickened and darkened-refractive; microcyclic
conidiogenesis occasionally occurring.
Culture characteristics: Colonies on PDA attaining 50−57 mm diam
after 14 d, grey-olivaceous, reverse grey-olivaceous to olivaceousgrey, paler parts olivaceous-buff, loccose to luffy-felty, margins
glabrous, somewhat shiny and slimy, white to olivaceous-buff, aerial
mycelium diffuse to somewhat dense, pale olivaceous-grey, loccose
to villose, growth low convex to dome-shaped with somewhat
elevated colony centre, without conspicuous exudates, sporulation
profuse. Colonies reaching 60−80 mm diam after 14 d, smoke-grey
123
BenSch et al.
Fig. 119. Cladosporium labelliforme (CBS 126345). A–H. Conidiophores and conidial chains. Scale bars = 10 µm.
to olivaceous, grey-olivaceous towards margins, reverse olivaceousgrey, loccose to luffy, margins white, glabrous to feathery radially
furrowed, aerial mycelium whitish forming dense patches, loccose to
felty, growth effuse, without prominent exudates, sporulation profuse.
Colonies on OA attaining 55−68 mm diam after 14 d, olivaceous-buff
to greenish olivaceous, reverse pale olivaceous-grey to olivaceousgrey, greyish sepia in colony centre, loccose, margins colourless,
glabrous, aerial mycelium sparse, diffuse, growth lat, somewhat
wrinkled, sometimes concentric zones of higher conidiophores and
areas of profuse sporulation, without exudates.
Substrate and distribution: On Melaleuca; Australia.
Notes: Cladosporium labelliforme, which clustered as a basal
sister to C. exile in phylogenetic analyses (Bensch et al. 2010, ig.
1, part a), belongs to the C. cladosporioides complex, but is well
distinguished from allied species by its conidial chains spread in
fan-like manner.
57. Cladosporium foliorum Ellis & Everh. ex K. Schub.,
Schlechtendalia 14: 65. 2006. Fig. 120, 121.
≡ Cladosporium foliorum Ellis & Everh., in herb.
Holotype: USA, California, Amador Co., Pine Grove, ca. 2200 m
alt., on living leaves of Angelica breweri (Apiaceae), Aug. 1896, G.
Hansen, No. 1362 (BPI 426581). Isotype: BPI 426580.
Lit.: Schubert (2005b: 70–71).
Ill.: Schubert (2005b: 71, ig. 24, pl. 9, igs F–I), Schubert et al.
(2006: 66, ig. 4, pl. 1, ig. F).
124
In vivo: Leaf spots amphigenous, formed as extended pale
ochraceous to yellowish orange discolorations, often limited by
leaf veins, appearing somewhat angular-irregular, at irst at leaf
margins, later spreading towards the midrib, covering large areas of
the leaf surface, later becoming somewhat darker, small segments
orange-brown or pale to medium brown and somewhat shiny,
without margin, rarely with a narrow brownish margin. Colonies
amphigenous, sparingly fruiting and not very conspicuous,
often at the tips of the leaves, loosely caespitose, pale brown to
brown. Mycelium internal, subcuticular to intraepidermal; hyphae
branched, 2–8 μm wide, septate, sometimes with small swellings
and constrictions, subhyaline to pale olivaceous-green, smooth or
almost so, walls slightly to distinctly thickened, radiating, forming
loose to dense stromatic hyphal plates (Fusicladium-like growth),
interconnecting stromata, hyphal cells angular, cylindrical-oblong,
polygonal, 5–10(–15) μm long, sometimes irregularly lobed, pale
yellowish to pale yellowish-brown. Stromata compact, 15–50 μm
diam, usually substomatal, composed of swollen hyphal cells, 3–10
μm wide, medium brown to somewhat reddish brown, smooth or
almost so, thick-walled. Conidiophores fasciculate, in small to
moderately large fascicles, arising from stromata, emerging through
stomata, rarely solitary, arising from hyphae, erect, substraight to
somewhat lexuous, narrowly cylindrical-oblong to iliform, often
apically slightly geniculate-sinuous, non-nodulose, unbranched
or once branched, 25–120 × 3–5 μm, 1–4-septate, septa not
very conspicuous, subhyaline to pale brown, almost smooth to
minutely verruculose throughout, walls one-layered, somewhat
thickened, sometimes slightly swollen at the base, up to 7 μm wide,
protoplasm of the cells somewhat aggregated at the septa, but not
very conspicuous. Conidiogenous cells integrated, terminal and
intercalary, cylindrical-oblong, often slightly geniculate, 13–51 μm
the genuS Cladosporium
darkened-refractive. Conidia catenate, in branched chains, straight
to slightly curved, subglobose, obovoid, ellipsoid to cylindrical, 4–26
× (2.5–)3.5–6 μm, 0–1(–2)-septate, sometimes slightly constricted
at the septa, pale brown, verruculose, walls slightly thickened,
apex and base rounded or somewhat attenuated, hila protuberant,
(0.5–)1–2 μm diam, thickened, somewhat darkened-refractive;
microcyclic conidiogenesis occurring.
Substrate and distribution: On Angelica breweri; USA.
Notes: With its radiating hyphal plates C. foliorum resembles species
of the genus Fusicladium, but it is quite distinct from the latter genus
in having cladosporioid conidiogenous loci and hila with a central
convex dome and a raised periclinal rim. Cladosporium dracaenatum
is morphologically close to C. foliorum but clearly separated by longer,
pluriseptate conidiophores and 0–3-septate conidia. With age the
conidia become more frequently septate (up to 7), longer and above
all wider, giving rise to secondarily formed conidiophores. Other
Cladosporium species described on a host belonging to Apiaceae
were excluded from this genus and reallocated to Passalora [e.g.
C. depressum ≡ Passalora depressa] or Pseudocercospora [C.
punctiforme = Pseudocercospora saniculae-europaeae].
Fig. 120. Cladosporium foliorum (BPI 426581). Fascicle of conidiophores and
conidia in vivo. Scale bar = 10 µm. K. Bensch del.
long, proliferation sympodial, with few conidiogenous loci situated
on small lateral shoulders, protuberant, well differentiated in a raised
dome and a periclinal rim, 1–2 μm diam, thickened, somewhat
58. Cladosporium fraxinicola K. Schub. & Mułenko,
Schlechtendalia 14: 67. 2006. Figs 122–125.
Holotype: Germany, Sachsen-Anhalt, Halle (Saale), Neuwerk/
Jägerplatz, Kindergarten, on Fraxinus excelsior (Oleaceae),
Fig. 121. Cladosporium foliorum (BPI 426581). A. Symptoms. B. Conidia. C. Conidiophores and conidia. D. Mycelium. Scale bars = 10 (B–D) µm.
www.studiesinmycology.org
125
BenSch et al.
23 Jun. 2004, K. Schubert (HAL 1829 F). Paratypes: Germany,
Sachsen-Anhalt, Halle (Saale), Neuwerk/Jägerplatz, Kindergarten,
on Fraxinus excelsior, 2 Aug. 2004, K. Schubert (HAL 1830 F) and
Braun, Fungi Sel. Exs. 47; Poland, Lublin, street margin, 10 Aug.
2004, W. Mułenko (HAL 1831 F) and Braun, Fungi Sel. Exs. 48.
Lit.: Schubert (2005b: 73).
Ill.: Schubert (2005b: 72–75, igs 25–27, pl. 12, igs A–H), Schubert
et al. (2006: 68–70, igs 5–7, pl. 1, igs H–I).
Exs.: Braun, Fungi Sel. Exs. 47, 48.
In vivo: On living leaves, causing leaf spots, amphigenous,
variable in shape and size, subcircular to oval-elliptical or
irregularly in outline, 3–8 mm wide, effuse, then usually irregular
in shape, up to 45 mm long or even longer, conluent, covering
large areas of the leaf surface, pale to medium brown or even
dark brown, somewhat darker in the centre, somewhat zonate,
surrounded by a narrow, irregular, dark brown or even reddish
brown margin, on the lower leaf surface spots paler. Colonies
epiphyllous or hypophyllous, scattered, punctiform, caespitose,
loose to somewhat denser, pale to dark brown or blackish, villose.
Mycelium internal and external, hyphae sometimes emerging
through stomata and growing supericially; hyphae sparingly
branched, 3.5–6(–8) μm wide, septate, subhyaline to very pale
olivaceous, smooth, walls only slightly thickened, often swollen,
swollen hyphal cells subglobose to somewhat angular, 7–14
μm wide, pale to medium olivaceous-brown. Stromata absent.
Conidiophores solitary or in small loose groups, arising from
internal and external hyphae or swollen hyphal cells, erumpent
through the cuticle, emerging through stomata or growing
supericially, erect, straight to slightly lexuous, often geniculatesinuous, somewhat subnodulose, unbranched or branched,
12–265 × (2.5–)4–9 μm, septate, pale olivaceous to olivaceousbrown, smooth, walls slightly to distinctly thickened, sometimes
even two-layered, slightly attenuated towards the apex, lumen
sometimes granular. Conidiogenous cells integrated, terminal
or intercalary or conidiophores reduced to conidiogenous cells,
10–52 μm long, proliferation sympodial, with a single to several
protuberant conidiogenous loci, 1–3 μm diam, thickened,
darkened-refractive. Conidia catenate, in branched chains,
straight, small terminal conidia (without apical hila) subglobose,
ovoid, obovoid to ellipsoid, 2.5–10 × 2–4.5 μm, intercalary conidia
and secondary ramoconidia limoniform, ellipsoid, fusiform to
cylindrical, 6.5–31 × 3.5–6 μm, 0–2(–3)-septate, rarely somewhat
constricted at the septa, pale olivaceous, smooth to minutely
verruculose, walls only slightly thickened, lumen sometimes
appearing to be granular, apex rounded or truncate, somewhat
attenuated towards the apex and base, base truncate to slightly
convex, hila (0.5–)1–3 μm diam, thickened, darkened-refractive;
microcyclic conidiogenesis occurring.
Fig. 122. Cladosporium fraxinicola (HAL 1829 F). Conidiophores and conidia in vivo.
Scale bar = 10 µm. K. Bensch del.
Substrate and distribution: On Fraxinus excelsior; Europe
(Germany, Poland).
Notes: Cladosporium fraxinicola is comparable with C. myrtacearum
(Braun et al. 2005) and C. psoraleae (Ellis 1976) but has deviating
characters of conidiophores, conidia and conidiogenous loci
(Schubert et al. 2006). Attempts to grow C. fraxinicola in culture
failed.
Several Cladosporium taxa have been described from Fraxinus
spp. Cladosporium acutum is conspeciic with C. herbarum.
Cladosporium desmotrichum (Desmazières 1851) was described to
126
Fig. 123. Cladosporium fraxinicola (HAL 1830 F, paratype). Symptoms,
conidiophores and conidia in vivo. Scale bar = 10 µm. K. Bensch del.
have fasciculate conidiophores with hyaline tips and 0–1-septate,
colourless conidia. Type material of this species could not be traced
at PC. Original material of C. fumago f. fraxini (nom. nud., Thümen,
Herb. Mycol. Oecon. 375, B 700006422) consists of green leaves
the genuS Cladosporium
infected with several hyphomycetes, including a Trimmatostromalike hyphomycete and C. herbarum. The taxonomic status of C.
simplex Schwein., described from the USA, is quite unclear since the
type material is too meagre and the original description (Schweinitz
1832) too brief and non-informative for a inal conclusion.
59. Cladosporium fumagineum Sacc., Nuovo Giorn. Bot.
Ital., N.S., 27: 86. 1920. Fig. 126, 127.
Lectotype (designated here): USA, Wyoming, Cook Co., Devils
Tower, on living leaves of Quercus sp. (Fagaceae), 28 Jul. 1918,
J.R. Weir, No. 10025 (PAD). Isolectotype: BPI 426763.
Lit.: Saccardo (1931: 791), Schubert (2005b: 76–77).
Ill.: Schubert (2005b: 76, ig. 28, pl. 13, igs A–C).
Fig. 124. Cladosporium fraxinicola (HAL 1831 F, paratype). Symptoms,
conidiophores, conidia and microcyclic conidiogenesis in vivo. Scale bar = 10 µm.
K. Bensch del.
Fig. 125. Cladosporium fraxinicola (HAL 1829 F and paratype HAL 1831 F). A,
B. Symptoms. C, D. Fascicle of conidiophores. E. Tip of a conidiophore with still
attached conidium and several conidia. F, H. Conidiophores. G. Conidial chain with
conidia just separating but still attached at the central domes. Scale bars = 10 (F–H),
20 (E), 50 (C–D) µm.
www.studiesinmycology.org
In vivo: Leaf spots amphigenous, oval to somewhat irregular,
often conluent, epiphyllous spots pale greyish brown to pale
reddish brown, margin indeinite or narrow and somewhat darker
reddish brown, sometimes surrounded by a narrow whitish grey
halo, hypophyllous olivaceous-brown to dark brown, surrounded
by a narrow pale yellowish to pale reddish brown halo. Colonies
hypophyllous, effuse, densely caespitose, dark brown, not veinlimited, velvety. Mycelium internal and external; hyphae branched,
2–5 μm wide, septate, often slightly constricted at the septa, pale
olivaceous to olivaceous-brown, smooth, walls slightly thickened,
often with small swellings, up to 7 μm wide, forming a loose hyphal
network. Conidiophores solitary or in small loose groups of 2–3,
arising from internal and external creeping hyphae, terminal and
lateral, erect, straight to somewhat lexuous, slightly geniculatesinuous towards the apex, unbranched or once branched, 14–150
× 3–5.5 μm, septate, olivaceous-brown, somewhat paler towards
the apex, smooth, walls thickened. Conidiogenous cells integrated,
terminal and intercalary, cylindrical, 7–25 μm long, somewhat
geniculate-sinuous, proliferation sympodial, with a single to several
conidiogenous loci, subdenticulate, truncate to slightly convex,
1–2 μm diam, thickened, darkened-refractive. Conidia catenate,
in unbranched or branched chains, straight to slightly curved,
subglobose, ovoid, obovoid, ellipsoid, fusiform to subcylindrical,
2.5–25 × 2.5–5.5 μm, 0–2(–3)-septate, sometimes constricted
Fig. 126. Cladosporium fumagineum (PAD). Conidiophores and conidia in vivo.
Scale bar = 10 µm. K. Bensch del.
127
BenSch et al.
Fig. 127. Cladosporium fumagineum (PAD). A. Conidiophores, somewhat geniculate-sinuous towards the apex. B, C. Conidia. Scale bars = 10 (A–C) µm.
at the septa, olivaceous to olivaceous-brown or medium brown,
smooth, walls only slightly thickened, apex often rounded, hila
protuberant, truncate to slightly convex, 0.5–2 μm diam, somewhat
thickened and darkened-refractive; microcyclic conidiogenesis not
observed.
Substrate and distribution: On Quercus sp.; USA.
Notes: This species is morphologically close to some collections
of C. uredinicola on Phyllactinia angulata on leaves of Quercus
sp. However, no powdery mildew fungi could be observed on
the leaves of the lectotype material, and leaf spots are formed.
Since C. uredinicola is a fairly variable, fungicolous species
mainly occurring on uredospores of several rust fungi, which
mostly produces much longer conidia (Heuchert et al. 2005),
C. fumagineum is tentatively maintained as a separate species.
Additional collections are needed to clarify the taxonomic status
of this taxon.
60. Cladosporium funiculosum W. Yamam., Sci. Rep.
Hyogo Univ. Agric., Ser. Agric. 4(1): 5. 1959. Figs 128, 129.
Holotype: Japan, isol. from leaves of Vigna umbellata
[= Phaseolus chrysanthos] (Fabaceae), probably authentic strain
of C. funiculosum. Ex-type culture: CBS 122129 = ATCC 38010
= IFO 6537 = JCM 10683.
Lit.: Bensch et al. (2010: 47−49).
Ill.: Bensch et al. (2010: 48, igs 34−35).
In vitro: Mycelium immersed and supericial, hyphae loosely
branched, iliform to cylindrical-oblong or irregular in outline due
to swellings, 1–3 μm wide, septate, smooth or mostly loosely
verruculose to densely verruculose Zasmidium(Stenella)-like,
walls unthickened, sometimes forming ropes. Conidiophores
micronematous to semimacronematous, solitary, arising terminally
and laterally from plagiotropous or ascending hyphae or hyphal
strains, iliform to narrowly cylindrical-oblong, neither geniculate
nor nodulose, unbranched, occasionally once branched,
10−120 × 2−3(−4) μm, usually rather short, 0−2(−5)-septate,
not constricted at septa, subhyaline to pale olivaceous, smooth
to minutely verruculose, walls unthickened, sometimes hardly
128
Fig. 128. Cladosporium funiculosum (CBS 122129). Macro- and micronematous
conidiophores, mycelium sometimes formed in ropes and conidial chains in vitro.
Scale bar = 10 µm. K. Bensch del.
distinguishable from hyphae, sometimes irregular in outline due
to swellings and constrictions. Conidiogenous cells integrated,
terminal, sometimes intercalary, proliferation often distinctly
sympodial, but neither geniculate nor nodulose, 10−33 μm long,
with 1−3 loci at the apex, sometimes few additional loci at a lower
level, subdenticulate, 1−1.5 μm diam, somewhat thickened and
darkened-refractive. Ramoconidia not formed. Conidia catenate,
in long unbranched or basely, often dichotomously branched
chains, up to 8(−14) conidia in the unbranched terminal part,
straight, small terminal conidia subglobose, obovoid, narrowly
ovoid, ellipsoid, sometimes narrowly obclavate, 2.5−5 × 1.5−2
μm (av. ± SD: 4.3 ± 1.0 × 1.7 ± 0.3), aseptate, intercalary conidia
narrowly ellipsoid, fusiform to subcylindrical, 5−16 × (1.5−)2−3
μm (av. ± SD: 9.3 ± 3.3 × 2.6 ± 0.4), 0−1-septate, with 1−2
distal hila, secondary ramoconidia ellipsoid to subcylindrical or
cylindrical, 7−23(−27) × 2.5−3.2(−4) μm (av. ± SD: 15.6 ± 5.1
× 2.9 ± 0.3), 0−1(−2)-septate, not constricted at septa, septum
often somewhat in the upper half, with (1−)2−3 distal hila, often
the genuS Cladosporium
Fig. 129. Cladosporium funiculosum (CBS 122129). A–E, G. Macronematous conidiophores and conidial chains. F. Micronematous conidiophore with conidial chains. Scale
bars = 10 µm.
with a second hilum near the base forming additional conidia
“backwards”, subhyaline to pale olivaceous, smooth, walls
unthickened, slightly to distinctly attenuated towards apex and
base, cell structure granular, hila conspicuous, subdenticulate,
0.5−1.5 μm diam, somewhat thickened and darkened-refractive;
microcyclic conidiogenesis not observed.
Culture characteristics: Colonies on PDA attaining 65−78 mm
diam after 14 d, glaucous-grey or iron-grey to black, olivaceous
towards margins, reverse greenish grey to grey-olivaceous or
dark mouse-grey to black, loccose, felty-woolly to shiny, margin
white to olivaceous, feathery, regular, aerial mycelium abundant,
loccose to villose, low to high, mainly in colony centre, growth
effuse to low convex, somewhat wrinkled, with numerous small to
large prominent exudates, sometimes coalescing, forming slimy
ring-like structures in colony centre, not sporulating. Colonies on
MEA reaching up to 80 mm diam after 14 d, pale olivaceous-grey
to buff or rosy-buff, reverse olivaceous-grey, brick to dark brick
towards margins, zonate, loccose to felty, margin white, glabrous
to feathery, narrow, regular, aerial mycelium abundant, covering
most of the colony surface, loccose to felty, dense, low, growth
effuse, radially furrowed and wrinkled, without prominent exudates,
not sporulating. Colonies on OA attaining 58−67 mm diam after
14 d, white to smoke-grey, pale olivaceous-grey or olivaceousgrey, colony centre buff or rosy-buff, reverse pale olivaceous-grey
to fawn, loccose to felty, margins colourless to white, glabrous,
regular, aerial mycelium abundant, covering the whole surface,
loccose to felty, growth lat, with numerous small prominent
exudates, not sporulating.
www.studiesinmycology.org
Substrate and distribution: On Vigna; Japan.
Notes: Due to smooth conidia, C. funiculosum belongs to the C.
cladosporioides complex. The history of description, typiication and
deposited cultures of this species is, however, somewhat confusing
and intricate. CBS strains of C. funiculosum are subcultures of
authentic strains deposited at the IFO culture collection. Bensch et
al. (2010) included two strains of C. funiculosum in the phylogenetic
studies of this complex. One of these (CBS 122128 = ATCC
16160 = IFO 6536 = JCM 10682) was named C. coralloides
and should represent an authentic strain of the latter species but
this seems to be a different species. The two strains are both
phylogenetically (see Bensch et al. 2010, ig. 1, part b as sister to C.
pseudocladosporioides) and morphologically identical whereas C.
coralloides, an invalidly published name isolated from Ficus carica
and Oryza sativa, is to be excluded from the genus Cladosporium
since the scar structure is not cladosporioid. A dried specimen
from BPI was re-examined agreeing with the species description
published in Yamamoto (1959) and shows Metulocladosporiellalike structures. The “lectotype” of C. coralloides in Ho et al. (1999)
was inappropriately designated since it was not an element from
the protologue of the original description. Furthermore, sequence
data of DNA from ATCC 16160 do not cluster with C. funiculosum
but represent another species. Conidiophore measurements and
the species epithet “funiculosum” introduced in Yamamoto (1959)
very probably refer to hyphal strands and not conidiophores since
these are often hardly distinguishable from hyphae or hyphal
strands.
129
BenSch et al.
61. Cladosporium fusicladiiformis Gonz. Frag., Trab. Mus.
Nac. Ci. Nat., Ser. Bot. 10: 188. 1916. Figs 130, 131.
Holotype: Spain, near Dos Hermanas, Sevilla, on living leaves of
Eriobotrya japonica (Rosaceae), 22 Mar. 1915, Gonzáles-Fragoso
(MA 06413).
Lit.: Gonzáles-Fragoso (1927: 205), Saccardo (1931: 795),
Schubert (2005b: 77–79).
Ill.: Schubert (2005b: 78, ig. 29, pl. 14, igs A–G).
In vivo: On living leaves, leaf spots amphigenous, numerous,
scattered, rarely somewhat oblong, up to 8 mm diam, later
conluent, later pale greyish brown by the detached cuticle, margin
indeinite or surrounded by a narrow, dark brown margin. Colonies
mostly hypophyllous, in small tufts, loosely scattered over leaf
spots, iliform, brown, villose. Mycelium internal, subcuticular to
intraepidermal, sometimes also external, growing supericially,
occasionally growing in leaf hairs; hyphae branched, (1–)2–
5(–7) μm wide, septate, often with swellings and constrictions,
subhyaline, pale to pale medium brown or somewhat yellowish
brown, smooth, walls somewhat thickened. Stromata usually welldeveloped, substomatal, occasionally intraepidermal, 20–50 μm
diam, few layers deep, composed of swollen hyphal cells, more
or less subglobose, 3–11 μm diam, medium brown or somewhat
yellowish brown, smooth, thick-walled. Conidiophores dimorphic,
fasciculate, usually in small loose fascicles (up to 10), arising from
stromata, mostly emerging through stomata, sometimes erumpent
through the cuticle, erect, more or less lexuous, cylindrical-oblong
to iliform, shorter conidiophores geniculate-sinuous, unbranched
or branched, 20–300 × 3–6.5(–8) μm, sometimes nodulose, with
small intercalar swellings, up to 7 μm diam, attenuated towards
the apex and slightly swollen at the base, up to 10 μm wide,
pluriseptate, sometimes slightly constricted at the septa, medium
brown, paler towards the apex, sometimes subhyaline at the apex,
smooth to somewhat asperulate, especially in the lower part, thickwalled, often distinctly two-layered, 0.5–1.5 μm wide, walls thinner
towards the apex, occasionally enteroblastically proliferating, cell
structure apparently distoseptate, with protoplasm aggregated
near the septa and a paler cavity in the centre of the cells; second
type of conidiophores shorter, narrower and paler, solitary, arising
from supericially growing hyphae or swollen hyphal cells, erect,
more or less straight, unbranched, 8–45 × 2–4 μm, septate, pale
olivaceous, smooth, walls somewhat thickened, not distinctly twolayered. Conidiogenous cells integrated, terminal and intercalary,
cylindrical-oblong, 5–30 μm long, proliferation sympodial, with few
to numerous, sometimes crowded conidiogenous loci, sometimes
situated on small lateral shoulders or intercalar and terminal
swellings, but not conined to them, somewhat protuberant,
truncate and lat to slightly convex, 1–2 μm diam, thickened,
often not darkened, but somewhat refractive. Ramoconidia not
observed. Conidia catenate, in branched chains, obovoid, ellipsoid,
fusiform, subcylin-drical, 3.5–14(–20) × 2.5–6 μm, 0–1(–2)-septate,
septa not very conspicuous, sometimes slightly constricted at
the septa, very pale, subhyaline to very pale brown, smooth or
almost so, occasionally minutely verruculose, walls somewhat
thickened, sometimes apparently two-layered, ends rounded or
slightly attenuated, hila more or less protuberant, slightly convex,
0.5–2 μm diam, thickened, refractive, sometimes slightly darkened;
microcyclic conidiogenesis sometimes observed.
130
Fig. 130. Cladosporium fusicladiiformis (MA 06413). Dimorphic conidiophores and
conidia in vivo. Scale bar = 10 µm. K. Bensch del.
Substrate and distribution: On Eriobotrya japonica; Spain.
Notes: In Saccardo (1931), C. fusicladiiformis was discussed as
being similar to Fusicladium eriobotryae, but the latter species, which
proved to be conspeciic with F. pomi (Schubert et al. 2003), is quite
distinct by having percurrently proliferating conidiogenous cells and
non-coronate conidiogenous loci. Furthermore, Saccardo (1931)
stated that C. eriobotryae and C. nervisequum, both described on
leaves of Eriobotrya japonica and antedating C. fusicladiiformis,
differ from the latter species. The conidia of C. nervisequum were
described to be oblong, 1–3-septate, longer and abobe all wider,
10–25 × 5–10 μm (Montagne 1857), but type material of the latter
species could not be traced and re-examined. The conidiophores
of C. eriobotryae, neotypiied above by a collection from Germany
(Botanical Garden, Berlin), are caespitose, usually unbranched
and short, 20–120 × 3–8 μm, and the conidia 1–3(–4)-septate,
verruculose and darker. Dimorphic conidiophores have not been
seen. Most of the other Cladosporium species described on hosts
belonging to the Rosaceae have been excluded from the genus
(Braun et al. 2003, Schubert et al. 2003, Schubert 2005b) or
proved to be synonyms of the widespread C. herbarum and C.
cladosporioides. Cladosporium diaphanum on Photinia glabra is
distinguished by having 0–6-septate, longer and wider conidia and
somewhat wider conidiogenous loci and hila.
Cladosporium apicale on Cycas circinalis and C. ushuwaiense
known from Argentina on Berberis ilicifolia are morphologically
comparable with C. fusicladiiformis but do not possess dimorphic
conidiophores. Furthermore, they deviate in having conidiophores
the genuS Cladosporium
almost so to asperulate or irregularly rough-walled, walls only slightly
thickened, < 1 μm wide, conidiophores often secede at septa forming
ramoconidia and fragments. Conidiogenous cells integrated, terminal
and intercalary, cylindrical-oblong, (11−)15−57 μm long, with 2−3 apical
subdenticulate loci, 1−1.8 μm diam, thickened and darkened-refractive.
Ramoconidia often formed, subcylindrical to cylindrical-oblong, 15−40
× 3−3.5(−4) μm, 0(−1)-septate. Conidia catenate, in branched chains,
branching in all directions, with up to ive conidia in the unbranched
part, small terminal conidia obovoid to fusiform, 3.5−5(–6.5) × 2−2.5(–
3) μm (av. ± SD: 3.9 ± 0.6 × 2.3 ± 0.3), intercalary conidia fusiform to
ellipsoid, 5−8(−9) × 2−3(−3.5) μm (av. ± SD: 6.5 ± 1.1 × 2.8 ± 0.4),
with up to three distal hila, secondary ramoconidia ellipsoid, fusiform
to subcylindrical or cylindrical, (7−)8−24(−31) × (2−)3−3.5(−4) μm (av.
± SD: 14.1 ± 5.5 × 3.2 ± 0.4), aseptate, with 2−3(−4) distal hila, pale
brown, smooth or almost so to minutely verruculose, walls unthickened
or only slightly thickened, attenuated towards apex and base, hila
conspicuous, 0.5−1.8 μm diam, thickened and darkened-refractive;
microcyclic conidiogenesis not occurring.
Fig. 131. Cladosporium fusicladiiformis (MA 06413). A. Symptoms. B. Overview,
fascicle of conidiophores. C. Dimorphic conidiophores, second type. D. Tip of
a conidiophore. E, G. Conidiophores showing cell structure, with thickened, twolayered walls and protoplasm aggregated at the septa. F. Conidia. Scale bars = 10
(C–G), 50 (B) µm.
with thicker walls [0.75–3 μm wide in C. apicale; 0.5–2(–3) μm wide
in C. ushuwaiense], the conidia of C. apicale are 0–3-septate, and
the conidia of C. ushuwaiense differ in being usually verruculose.
62. Cladosporium fusiforme Zalar, de Hoog & GundeCimerman, Stud. Mycol. 58: 169. 2007. Fig. 132.
Holotype: Slovenia, Sečovlje salterns, isol. from hypersaline water,
Dec. 1999, L. Butinar (CBS H-19732). Ex-type culture: EXF-449
= CBS 119414.
Ill.: Zalar et al. (2007: 171, ig. 7).
In vitro: Mycelium sparingly branched, 1−4 μm wide, septate, pale to
medium olivaceous-brown, sometimes subhyaline, minutely verruculose
or smooth, walls unthickened, without extracellular polysaccharidelike material. Conidiophores solitary, semimacronematous to
macronematous, arising terminally and laterally from hyphae, erect,
straight to slightly lexuous, cylindrical-oblong to iliform, neither
nodulose nor geniculate, unbranched to branched, branches often
only as short denticle-like lateral prolongation just below a septum,
sometimes almost every single cell with a peg-like lateral outgrowth,
often to the same direction, (10−)20−170(−200) × (2−)2.5−3.5(−5) μm,
regularly septate with 0−8 septa, not constricted at the often darkened
septa, subhyaline, pale to medium olivaceous-brown, smooth or
www.studiesinmycology.org
Culture characteristics: Colonies on PDA reaching 32−46 mm diam
after 1 month, grey-olivaceous to dark herbage-green at margins,
reverse leaden-grey to iron-grey, velvety, margin narrow, feathery,
aerial mycelium sparse, hairy, diffuse, growth lat or sometimes
with elevated colony centre, without prominent exudates. Colonies
on MEA reaching 38 mm diam after 1 month, pale olivaceous to
olivaceous or grey-olivaceous, reverse olivaceous-grey to iron-grey,
velvety to powdery, sometimes felty, margin narrow, white, feathery,
regular, radially furrowed, aerial mycelium sparse, felty, loose, high,
without prominent exudates. Colonies on MEA + 5 % NaCl reaching
28–43 mm diam, olive, granular due to profuse sporulation, slightly
furrowed with lat, olive-grey margin, reverse dark green. Colonies
on OA attaining 53−64 mm diam after 1 month, pale olivaceous to
olivaceous, reverse iron-grey to leaden-grey, velvety to powdery,
margin white, feathery, aerial mycelium absent or sparse, growth
lat, prominent exudates not formed, sporulation profuse on all
media. Maximum tolerated salt concentration: 75 % of tested
strains develop colonies at 20 % NaCl after 7 d, while after 14 d
all strains grow and sporulate. Cardinal temperatures: No growth
at 4 and 10 °C, optimum 25 °C (30–32 mm diam), maximum 30
°C (2–15 mm diam), no growth at 37 °C (from Zalar et al. 2007).
Substrates and distribution: Saprobic, isolated from chicken food
(Canada), hypersaline water (Slovenia) and on chasmothecia of
powdery mildew fungi (USA).
Additional specimens examined: Canada, Saskatchewan, Saskatoon, isol. from
chicken food by Riddell (CBS 452.71). Slovenia, Sečovlje saltern, isol. from
hypersaline water (EXF-397). USA, Washington, Seattle, University of Washington
campus, isol. from chasmothecia of Phyllactinia guttata (Erysiphales) on leaves of
Corylus avellana (Betulaceae), 12 Feb. 2004, D. Glawe (CPC 11835).
Notes: This species was treated by Zalar et al. (2007) within the
C. sphaerospermum complex, but differs from other taxa of this
complex in having fusiform terminal conidia and usually unbranched
conidiophores.
63. Cladosporium galii Mułenko, K. Schub. & M. Kozłowska,
Mycotaxon 90(2): 272. 2004. Figs 133, 134.
Holotype: Poland, Nizina Północnopodlaska, Białowieża Forest,
Białowieża National Park, Forest Compartment 342, Permanent
131
BenSch et al.
Fig. 132. Cladosporium fusiforme (CBS 119414) (from Zalar et al. 2007). A–D. Colony surface grown on PDA (A), OA (B), MEA (C) and MEA plus 5 % NaCl (D) of strains
incubated for 14 d at 25 ºC in darkness. E–G. Habit of conidiophores. H–I. Ramoconidia and conidia. E–I. All from 7-d-old SNA slide cultures. A–H, from EXF-449 (ex-type strain);
I, from CBS 452.71. Scale bars = 10 (H–I), 30 (F–G), 100 (E) µm.
plot No 40 BSG UW, single collection in oak-linden-hornbeam
forest (Tilio-Carpinetum), on living leaves of Galium odoratum
(Rubiaceae), 26 Sep. 1992, W. Mułenko (LBLM–8459). Isotype:
HAL 1811 F.
132
Lit.: Schubert (2005b: 79–81).
Ill.: Mułenko et al. (2004: 273, ig. 1), Schubert (2005b: 80, ig. 30,
pl. 13, igs D–F).
the genuS Cladosporium
In vivo: On living leaves, distinct leaf spots lacking, but with pale
olivaceous-brown to greyish discolorations. Colonies hypophyllous,
rarely epiphyllous, punctiform, in small tufts, scattered, pale to dark
brown, sometimes almost blackish. Mycelium internal, subcuticular
to subepidermal, immersed; hyphae branched, (3–)4–8(–10) μm
wide, septate, with swellings and constrictions, pale to medium
brown, smooth, walls slightly thickened, forming loose to somewhat
denser stromatic hyphal aggregations. Stromata 37.5–82.5(–100)
μm diam, composed of swollen, subcircular, ellipsoid to somewhat
angular-irregular, thick-walled hyphal cells, (5–)7–16(–20) μm wide,
olivaceous to dark brown, smooth. Conidiophores solitary, arising
from hyphae or in loose to dense fascicles arising from stromatic
hyphal aggregations, erumpent through the cuticle or emerging
through stomata, erect, straight or slightly lexuous, unbranched
or rarely branched, 25–280 × (2.5–)4.5–8(–10) μm, septate, but
only few septa (up to four), subhyaline, pale to medium olivaceousbrown, somewhat paler towards the apex, smooth, sometimes
minutely verruculose at the apex, walls slightly thickened, more
thick-walled near the base, sometimes even two-layered, usually
swollen and somewhat darker at the base, up to 12 μm wide, often
with small, head-like, terminal swellings, up to 8 μm wide, with a
single or several distinct scars at the apex. Conidiogenous cells
integrated, terminal or intercalary, cylindrical or often with small
swellings, 18–80 μm long, proliferation sympodial, with a single or
few conidiogenous loci, often situated on swellings but not restricted
to them, protuberant, mostly short cylindrical, (1–)1.5–2.5 μm diam,
somewhat thickened and darkened–refractive. Conidia catenate, in
simple or branched acropetal chains, sometimes solitary, straight to
slightly curved, primary conidia small, obovoid, ellipsoid, 3–7 × 2–4
μm, smooth, secondary conidia ellipsoid, fusiform to cylindrical,
6–30(–40) × (2.5–)3–6.5 μm, mostly 0(–2)-, very rarely 3-septate,
not constricted at the septa, subhyaline, very pale to medium
pale brown, smooth to usually minutely verruculose, walls only
slightly thickened, somewhat rounded or attenuated at the ends,
with protuberant hila at one end or both ends, slightly convex,
(0.5–)1–2.5 μm diam, well differentiated in a central convex dome,
surrounded by a raised ine rim, thickened, somewhat darkenedrefractive.
Substrate and distribution: On Galium odoratum; Poland.
Notes: Cladosporium pilicola (Saccardo 1892: 602) was described
from France on dry stems of Galium mollugo. Type material of this
species could not be traced, and other collections are unknown.
However, based on the original description, C. pilicola is quite
Fig. 133. Cladosporium galii (HAL 1811 F). Conidiophores and conidia in vivo. Scale
bar = 10 µm. K. Bensch del.
distinct from C. galii (probably saprobic; conidiophores branched;
conidia cylindrical, 1–3-septate). Cladosporium cladosporioides
is morphologically close to C. galii, but differs in having narrower,
cylindrical conidiophores, 2–5.5 μm wide, without swellings, usually
formed singly, and usually smooth, narrower conidia, 2–5 μm wide.
Nodulose conidiophores are known from some other Cladosporium
species, e.g., C. colocasiae, C. herbarum, C. macrocarpum, C.
oxysporum and C. variabile. The conidia of C. herbarum and C.
variabile are coarsely verruculose and wider. In addition, C. variabile,
conined to Spinacia oleracea, is well-distinguished by forming
tortuose, spirally twisted aerial hyphae. Cladosporium colocasiae,
a common parasite of Colocasia species, has much wider, usually
Fig. 134. Cladosporium galii (HAL 1811 F). A. Conidiophores. B. Conidiogenesis. C. Conidium. Scale bars = 10 (A–C) µm.
www.studiesinmycology.org
133
BenSch et al.
6–9 μm broad, smooth conidia, and the widespread saprobic
species C. oxysporum differs in having very long conidiophores, up
to 500 μm long or even longer, and smooth conidia.
64. Cladosporium gallicola B. Sutton, Mycol. Pap. 132: 37.
1973. Figs 135–137.
Holotype: Canada, Saskatchewan, 27’S. Meadow Lake, on galls
of Endocronartium harknessii (Cronartiaceae) on twigs of Pinus
banksiana (Pinaceae), 25 May 1967, C. Rentz, WINF (M) 6898e
(IMI 145204).
Lit.: Ellis (1976: 329), Heuchert et al. (2005: 28–33).
Ill.: Sutton (1973: 38–39, figs 17, 18), Ellis (1976: 329, ig. 247),
Heuchert et al. (2005: 29, ig. 8).
In vivo: Colonies on galls and intact as well as empty aecia,
medium olivaceous-brown to dark brown, in loose to dense
fascicles or solitary, spreading to the surrounding tissue of the
host plant, effuse, caespitose, velvety, conluent, affected needles
discoloured, reddish brown. Mycelium immersed and external,
supericial; hyphae branched, 1–4(–8) μm wide, cells 7–15 μm
long, often irregularly swollen, 5–7 μm diam, pale to medium brown,
occasionally subhyaline. Stromata hemisphaerical, 45–130 × 30–90
μm, composed of subglobose to subangular cells, 4–12 μm diam,
dark brown, smooth. Conidiophores solitary, arising from hyphae,
or mostly formed in loose to dense fascicles, mostly more than 10,
arising from stromata, erumpent, erect, straight to slightly curved,
subcylindrical, 34–260 × (3.5–)6–9 μm, at the base up to 10 μm wide,
somewhat attenuated towards the apex, up to 15-septate, usually not
constricted at the septa, medium to dark brown, paler towards the
apex, smooth to faintly verruculose, walls thickened, less thickened
or unthickened towards the apex, occasionally enteroblastically
proliferating, monopodial, usually branched in the upper third,
branchlets 11–43(–65) × 4–5(–7) μm, 0–3(–5)-septate, subhyaline to
pale brown. Conidiogenous cells integrated, terminal and intercalary,
subcylindrical or somewhat attenuated towards the tip, 6–35 μm
long, polyblastic, with numerous conspicuous conidiogenous loci
(up to 10 or even more), proliferation sympodial, appearing to be
coarsely verrucose by numerous densely arranged protuberant
conidiogenous scars, loci slightly to conspicuously protuberant,
truncate, denticle-like, somewhat thickened and refractive, 1–3 μm
diam, convex dome sometimes indistinct. Ramoconidia lacking.
Conidia usually in branched chains, straight, small terminal conidia
subglobose, obovoid, limoniform, ellipsoid, fusiform to broadly
subcylindrical, 3–17 × 2–6(–7) μm, 0(–1)-septate, without any
constrictions, subhyaline to pale brown, smooth, walls thin to
slightly thickened, intercalary conidia and secondary ramoconidia
broadly ellipsoid to subcylindrical, 12–29 × 6–8 μm, 2–3(–4)-septate,
occasionally constricted at the septa, medium brown, smooth, walls
thickened, apex rounded or provided with up to six hila, base rounded
to attenuated, truncate, more or less protuberant, slightly thickened
and refractive, convex dome sometimes not very distinct, (0.5–)1–2.5
μm diam; microcyclic conidiogenesis occurring.
Substrates and distribution: On rust fungi (Uredinales); North
America – Cronartium comandrae on Pinus contorta var. latifolia
(Canada, widespread), Endocronartium harknessii on Pinus
banksiana (Canada, widespread), Pucciniastrum goeppertianum
on Abies grandis (USA, WA).
134
Fig. 135. Cladosporium gallicola (IMI 145204). Conidiophores, conidiogenous cells
and conidia in vivo. Scale bar = 10 µm. B. Heuchert del.
Fig. 136. Cladosporium gallicola (BPI, as “C. peridermiicola”). Dense fascicle of
conidiophores arising from stromata, conidiophores arising from hyphae and conidia
in vivo. Scale bar = 10 µm. B. Heuchert del.
the genuS Cladosporium
Fig. 137. Cladosporium gallicola (BPI, as “C. peridermiicola”). A–D. Conidiophores with darkened-refractive conidiogenous loci. E. Dense fascicle of commonly branched
conidiophores. F. Details of scar structure on a conidiophore and secondary ramoconidium. Scale bars = 5 (F), 10 (A–D), 50 (E) µm.
Additional specimens examined: USA, Washington, Metalline Falls, on Peridermium
columnare (= Pucciniastrum goeppertianum) on Abies grandis, 6 Jun. 1930, G.G.
Hedgcock (BPI 427386, deposited as C. peridermiicola in herb.); Sullivan Lake, 9
Jun. 1930, G.G. Hedgcock (BPI 427385); 5 Jul. 1929 (BPI 427383).
Notes: Under light microscopy, the cladosporioid structure of the
conidiogenous loci with distinct central dome and periclinal raised
rim was not quite evident, but SEM (Heuchert et al. 2005, pl. 2, ig.
8) conclusively showed that C. gallicola belongs in Cladosporium s.
str. Strongly branched conidiophores, as described and depicted by
Sutton (1973), could be found in the type collection, but were less
branched in the other specimens examined. Several specimens
deposited at BPI under the herbarium name C. peridermiicola
proved to be identical with C. gallicola. Stromata are present
and the conidia are broadly ellipsoid-subcylindrical, 12–29 × 6–8
μm, 2–3(–4)-septate, occasionally somewhat constricted at the
septa, but otherwise these collections agree well with C. gallicola.
Records of Cladosporium sp. on Cronartium conigenum and C.
coleosporioides from Canada, Alberta, by Powell (1971) possibly
belong to C. gallicola.
65. Cladosporium gamsianum Bensch, Crous & U. Braun,
Stud. Mycol. 67: 49. 2010. Figs 138, 139.
Fig. 138. Cladosporium gamsianum (CBS 125989). Conidiophores and conidial
chains in vitro. Scale bar = 10 µm. K. Bensch del.
Holotype: South Africa, Pretoria, Walter Sisulu Botanical Garden,
25.706944, 28.229444, isol. from Strelitzia sp. (Strelitziaceae), 17
Feb. 2005, coll. W. Gams, isol. P.W. Crous (CBS H-20434). Ex-type
culture: CBS 125989 = CPC 11807.
forming ropes. Conidiophores solitary or in small groups of four
or sometimes fasciculate, macronematous, arising terminally and
laterally from hyphae or hyphal ropes, erect, straight or slightly
lexuous, cylindrical-oblong, not geniculate, sometimes slightly
swollen at the apex, sometimes with monopodial rejuvenations
(monopodially proliferating without conidiogenesis) and a single
rather inconspicuous annellation, unbranched, 10−146 × 3−5
μm, pluriseptate, sometimes slightly constricted at septa, medium
olivaceous-brown, paler towards or at the uppermost apex, with
age dark brown, more thick-walled and two-layered, walls about
1 μm wide, slightly attenuated towards the apex, base sometimes
up to 6.5 μm wide. Conidiogenous cells integrated, sympodially
Ill.: Bensch et al. (2010: 50, igs 36−37).
In vitro: Mycelium sparingly branched, 1−3.5 μm wide, at the base
of conidiophores wider, up to 5 μm wide, septate, subhyaline to pale
olivaceous or pale olivaceous-brown, with age hyphae becoming
dark brown, smooth, sometimes constricted at septa and minutely
verruculose towards the base of conidiophores, walls unthickened,
www.studiesinmycology.org
135
BenSch et al.
Fig. 139. Cladosporium gamsianum (CBS 125989). A–G. Conidiophores and conidial chains. Scale bars = 10 µm.
proliferating, usually terminal, with age intercalary, cylindricaloblong, not geniculate, with age slightly swollen, subnodulose at
or towards the apex with loci situated at these lateral shoulders,
9−18 μm long, rupturing the outer wall layer around some of the
scars, resulting in a lateral displacement of scars, leaving more
or less conspicuous circumferential annular fringes of the torn
wall, lateral scars in face view conspicuous, lat, non-protuberant
or only slightly so, slightly thickened and darkened, with 1−4 loci
at the apex, with age up to eight loci crowded towards the apex,
loci conspicuous, subdenticulate, 1−1.5(−1.8) μm diam, thickened
and darkened-refractive. Conidia catenate, in branched chains,
branching dichotomously or in all directions, narrow, straight, small
terminal conidia obovoid, 3−6 × 1−1.5(−2) μm (av. ± SD: 5.0 ±
0.9 × 1.4 ± 0.3), aseptate, intercalary conidia fusiform to narrowly
ellipsoid-ovoid, 4.5−12 × 1.5−2.5 μm (av. ± SD: 7.4 ± 2.0 × 1.9
± 0.4), aseptate, with 1−3(−5) distal hila, secondary ramoconidia
narrowly ellipsoid to subcylindrical, (6−)7−14.5 × (1.2−)1.5−3(−3.5)
μm (av. ± SD: 10.3 ± 2.4 × 2.6 ± 0.6), 0(−1)-septate, sometimes
constricted at the median septum, subhyaline to pale olivaceous
or pale olivaceous-brown, smooth, walls unthickened or almost
so, attenuated towards apex and base, with 1−5 distal hila,
conspicuous, subdenticulate, 0.5−1.5(−1.8) μm diam, thickened
and darkened-refractive; microcyclic conidiogenesis not observed.
Culture characteristics: Colonies on PDA olivaceous-grey to irongrey, grey-olivaceous towards margins, somewhat zonate, reverse
olivaceous-grey to greyish-blue, grey-olivaceous towards margins,
woolly-felty, margin white, glabrous, aerial mycelium abundant,
covering almost the whole colony surface, woolly-felty, sometimes
high, growth lat, numerous small or large prominent exudates
formed, sporulation profuse. Colonies on MEA olivaceous-grey,
136
iron-grey or black towards margins, reverse iron-grey, velvety to
woolly, margin white, glabrous, sometimes radially furrowed, aerial
mycelium abundant, covering the whole colony surface, growth low
convex, without prominent exudates, sporulating. Colonies on OA
olivaceous to grey-olivaceous, olivaceous-grey towards margins,
reverse leaden-grey to leaden-black, velvety to powdery, margin
white, narrow, glabrous, aerial mycelium absent or sparse, growth
lat, sporulation profuse.
Substrate and distribution: On Strelitzia; South Africa.
Notes: Cladosporium gamsianum, which is an element of
the C. cladosporioides complex, clustered as a sister to C.
verrucocladosporioides (Bensch et al. 2010, ig. 1, part b) but
formed a separate lineage in all phylogenetic analyses. This
species is unique within the genus Cladosporium by its special
mode of rejuvenation leaving more or less conspicuous annellations
and conspicuous lateral conidiogenous loci that are somewhat
reminiscent of the genus Annellosympodia (McTaggart et al. 2007).
66. Cladosporium gentianae Lobik, Bolezni Rast. 17(3–4):
189. 1928. Figs 140, 141.
Holotype: Russia, on leaves of Gentiana cruciata (Gentianaceae),
15 Sep. 1927 (LE 40527).
Lit.: Shvartsman et al. (1975: 95), Schubert (2005b: 81–83).
Ill.: Lobik (1928: tab. 8, ig. 87), Schubert (2005b: 82, ig. 31, pl. 13,
igs G–J).
the genuS Cladosporium
In vivo: On living leaves, leaf sheaths and stems, leaf spots at
leaf margins or starting at leaf margins and extending towards
the mid, sometimes even covering the whole leaf surface, fading,
turning yellowish brown, ochraceous, margin indeinite, somewhat
raised at the border to the healthy leaf tissue, stems yellowish
brownish discoloured, fading. Colonies amphigenous, solitary
or in small tufts, effuse, scattered, loosely to moderately dense,
caespitose, dark brown to blackish. Mycelium internal, subcuticular
to intraepidermal; hyphae conspicuous, sparingly branched, 3.5–11
μm wide, septate, sometimes distinctly constricted at the septa,
subhyaline, pale olivaceous to medium olivaceous-brown, smooth
or almost so to minutely verruculose, thick-walled, wall appearing
yellowish, somewhat darker than the lumen and refractive, often
with small to large, subglobose to somewhat irregular oil droplets,
forming hyphal aggregations and stromata. Stromata substomatal
to intraepidermal, small, subglobose, later somewhat lattened,
15–50 μm diam, only few layers deep, usually composed of only
few but large swollen hyphal cells, up to 20 μm wide, subglobose
to somewhat angular or irregular, medium to medium dark
olivaceous-brown or brown, rarely pale olivaceous-brown, smooth
to minutely verruculose, thick-walled. Conidiophores mostly loosely
fasciculate, with 2–8 per fascicle, arising from stromata, sometimes
solitary, arising from internal hyphae, emerging through stomata or
erumpent through the cuticle, erect, rarely subdecumbent, more or
less straight, cylindrical-oblong, robust, non-nodulose, usually not
geniculate, unbranched, 26–120(–180) × (4–)5–10 μm, septate,
not constricted at the septa, dark olivaceous-brown to brown,
Fig. 140. Cladosporium gentianae (LE 40527). Conidiophores and conidia in vivo.
Scale bar = 10 µm. K. Bensch del.
minutely verruculose or somewhat irregularly rough-walled, walls
thickened, usually distinctly two-layered, 1–1.5 μm wide, slightly
attenuated towards the apex, sometimes somewhat swollen at
the base, protoplasm somewhat aggregated near the septa, which
Fig. 141. Cladosporium gentianae (LE 40527). A. Overview, fascicle of conidiophores. B. Conidiophore. C. Conidiophore and conidia. D. Mycelium. Scale bars = 10 (B–D), 20
(A) µm.
www.studiesinmycology.org
137
BenSch et al.
appear to be thickened, similar to distoseptation, occasionally
enteroblastically proliferating. Conidiogenous cells integrated,
terminal, occasionally intercalary, 13–36 μm long, proliferation
sympodial, with 1–8 conidiogenous loci, often crowded at the
apex, protuberant, short cylindrical, subdenticulate, 1.5–2(–2.5) μm
diam, thickened, somewhat darkened-refractive. Conidia catenate,
usually in branched chains, straight, subglobose, obovoid, ovoid,
narrowly to broadly ellipsoid to subcylindrical, 3.5–23 × 3–6(–8)
μm, 0–1(–2)-septate, septum more or less median, not constricted
at the septa, pale olivaceous-green to olivaceous-brown or brown,
smooth or almost so to verruculose or irregularly rough-walled,
walls thickened, up to 1 μm wide, rarely appearing two-layered,
often with a distinct, clearly delineated lumen, apex rounded or with
up to 4 hila, slightly attenuated towards the base, hila protuberant,
short cylindrical, 1–2 μm diam, thickened, somewhat darkenedrefractive; microcyclic conidiogenesis not observed.
Substrates and distribution: On Gentiana spp. (Gentianaceae),
Europe – Gentiana cruciata (Kazakhstan, Russia).
Notes: Lobik (1928) described and illustrated up to 3-septate
conidia, which could not be observed. Cladosporium gentianae
is the only species in this genus known to attack a member
of the Gentianaceae. The seta-like, non-nodulose, very dark
conidiophores and the conspicuous, often wide hyphae chiely
characterise this species, deining and distinguishing it from
morphologically allied taxa. Cladosporium episclerotiale known
from galls of Endocronartium harknessii possesses longer
conidiophores, (22–)97–295(–322) μm, with frequently branched
apices and 0–4-septate conidia (Heuchert et al. 2005), and C.
populicola differs in having shorter, obovoid-ellipsoid conidia, 4–14
μm long, and narrower hyphae, (2–)3–4.5 μm wide.
67. Cladosporium gerwasiae Heuchert, U. Braun & K.
Schub., Schlechtendalia 13: 31. 2005. Figs 142, 143.
Holotype: Guatemala, Chimaltenango, Tecpan, Xecoxol, “plantation
ex Rubus urticifolius”, N 140°51.8; W 90°59, on Gerwasia sp.
(Uredinales) on leaves of Rubus urticifolius (Rosaceae), G.A.
Alvarez (KR-5684).
Ill.: Heuchert et al. (2005: 32, ig. 10 and pl. 1, ig. 1–2).
In vivo: Hyperparasitic. Colonies conined to epiphyllous sori of
Gerwasia sp. and the surrounding deformed leaf tissue, but not
spreading onto green leaf tissue which is not affected by the rust,
dark olivaceous-brown, visible (at 10–50×) as diffuse to mostly
dense tufts. Mycelium immersed and external, supericial; hyphae
sparingly branched, 2–6 μm wide, septate, often slightly constricted
at the septa, pale brown, smooth, walls slightly thickened or
unthickened. Stromata formed by swollen hyphal cells, subglobose
to somewhat angular, 4–9 μm diam, pale to medium brown, walls
slightly thickened. Conidiophores in loose to dense fascicles, arising
from stromata, or solitary, arising from swollen hyphal cells, erect,
straight to curved, cylindrical, unbranched or branched at the base
as well as near the tips, 24–366(–630) × (3–)4–7(–8) μm, often
somewhat wider near the base, tips often elongated and narrowed,
only 2.5 μm wide, 1–22-septate, not or only slightly constricted at
the base, pale to medium brown, tips paler, occasionally subhyaline,
smooth, occasionally minutely rough-walled near the base,
138
Fig. 142. Cladosporium gerwasiae (KR-5684). Conidiophores in loose to dense
fascicles arising from stromata and conidiophores arising from swollen hyphal
cells, conidiogenous cells, ramoconidia and conidia in vivo. Scale bar = 10 µm.
B. Heuchert del.
pigmented walls thickened, (0.5–)1–1.25 μm wide, often distinctly
two-layered towards the base, unthickened and one-layered
above, lumen often distinct, diminished, granular, surrounded by
a wide, colourless to greyish layer (under phase-contrast), giving
a very thick-walled appearance, some cells of the conidiophores
conspicuously attenuated. Conidiogenous cells integrated, terminal
and intercalary, cylindrical, 7–50 μm long, polyblastic, proliferation
subtly to markedly sympodial, conidiogenous loci protuberant,
almost denticle-like, more or less thickened, darkened-refractive,
1.5–2.5 μm diam. Ramoconidia 14–26 × 3–5 μm, 0–2(–3)-septate,
base slightly convex, without a coronate hilum, up to 3 μm
wide. Conidia usually in branched chains, ellipsoid, obovoid,
subcylindrical, 4–24 × 2.5–8 μm, 0–1(–3)-septate, not or only
slightly constricted at the septa, very pale brown, smooth, external
pigmented wall layer slightly thickened, with phase-contrast
showing a granular lumen surrounded by a distinct hyaline to
greyish layer, giving a thick-walled appearance, apex rounded to
slightly attenuated, with up to four hila, base truncate to convex,
occasionally somewhat attenuated, hila protuberant, more or less
thickened and conspicuously darkened-refractive, 0.5–2.5 μm
diam; microcyclic conidiogenesis occurring.
Substrate and distribution: On Gerwasia sp.; Guatemala.
Notes: This species is easily distinguishable from C. uredinicola
by its obviously fasciculate habit, usually wider conidiophores, 4–7
μm, with thicker, often two-layered walls, (0.5–)1–1.25 μm wide.
Colonies of C. uredinicola are effuse, consistently non-fasciculate,
the conidiophores are 2–5 μm wide, thin-walled, 0.5–0.75 μm,
and not two-layered. Cladosporium gallicola, also occurring on
the genuS Cladosporium
Fig. 143. Cladosporium gerwasiae (KR-5684). A. Tip of a conidiophore with still attached conidia. B. Conidiophores intermixed with uredospores of Gerwasia. C. Overview
showing fasciculate conidiophores and conidia in vivo. D. Conidia. Scale bars = 10 (A–B, D), 50 (C) µm.
rust fungi, differs in having strongly branched conidiophores with
conidiogenous cells which are seemingly coarsely verrucose
by being densely covered with numerous conidiogenous loci.
Cladosporium aecidiicola has verruculose conidia (Heuchert et al.
2005).
68. Cladosporium gleditschiae Cooke, Grevillea 17(83):
66. 1889. Figs 144, 145.
Lectotype (designated here): USA, South Carolina, Aiken, on
legumes of Gleditsia sp. (Fabaceae), Ravenel, Fungi Amer. Exs.
297 (K 121559). Isolectotype: BPI 426765, NY, PH.
Lit.: Saccardo (1892: 603, 1895: 621).
Exs.: Ravenel, Fungi Amer. Exs. 297.
In vivo: On pods, causing sometimes discolorations or small spots.
Colonies caespitose, effuse, loose to dense, dark olivaceous-brown
to brown or almost blackish, velvety or somewhat crustaceous,
covering large areas of the pod surface. Mycelium internal and
external, supericial, hyphae creeping, branched, 3–5(–6) μm
wide, septate, with swellings and constrictions, pale to medium or
even dark brown, smooth to minutely verruculose, thick-walled,
forming extended, dense stromatic hyphal aggregations, swollen
hyphal cells subglobose, 5.5–10 μm wide, protoplasm often
aggregated at walls and septa forming a small to somewhat large
cavity in the centre, sometimes forming dense, compact, medium
to dark brown conglomerations. Conidiophores solitary or in loose
groups of 2–3, arising from hyphae or swollen hyphal cells, erect
to subdecumbent, straight to lexuous, often geniculate-sinuous,
non-nodulose, unbranched or branched, 16–105 × (3.5–)4–6 μm,
pluriseptate, sometimes slightly constricted at septa, pale to medium
brown, often somewhat paler towards the apex, smooth or almost
so to minutely verruculose or rugose, thick-walled, often somewhat
swollen at the base, up to 8 μm wide, attenuated towards the apex,
protoplasm often aggregated at walls and septa forming a distinct
lumen in the centre, sometimes forming distosepta. Conidiogenous
cells integrated, terminal or intercalary, short cylindrical to oblong,
geniculate-sinuous, 4–18 μm long, proliferation sympodial, with a
single or a few conidiogenous loci, protuberant, subdenticulate, 1–2
μm diam, thickened, darkened-refractive. Conidia catenate, in simple
or branched chains, straight, obovoid, broadly ellipsoid, fusiform to
cylindrical, 5–28.5 × 3.5–8.5 μm, 0–3(–4)-septate, sometimes slightly
constricted at septa, rarely with 1–2 additional transversal septa, pale
to medium brown, smooth to minutely verruculose or rugose, walls
www.studiesinmycology.org
Fig. 144. Cladosporium gleditschiae (K 121559). Conidiophores arising from
swollen hyphal cells, conidia and microcyclic conidiogenesis in vivo. Scale bar =
10 µm. K. Bensch del.
slightly thickened, apex rounded or somewhat attenuated towards
apex and base, protoplasm often aggregated at walls and septa
forming a distinct lumen in the centre, hila more or less protuberant,
truncate to slightly convex, 1–2 μm diam, thickened, darkenedrefractive; microcyclic conidiogenesis occurring.
Substrate and distribution: On Gleditsia sp.; USA.
Notes: The species has also been reported on Gleditsia triacanthos
from Turkmenistan (Koshkelova & Frolov 1973). Cladosporium
vignae, causal agent of the scab, leaf and pod blight on Vigna
and Lespedeza spp., is morphologically comparable but distinct in
having 0–1-septate, narrower conidia (2–6 μm).
69. Cladosporium globisporum Bensch, Crous & U. Braun,
Stud. Mycol. 67: 51. 2010. Figs 146–148.
Holotype: Sweden, isol. from meat stamp, 1986, M. Olsen, No.
M291 (CBS H-20435). Ex-type culture: CBS 812.96.
Ill.: Bensch et al. (2010: 51−53, igs 38−40).
139
BenSch et al.
Fig. 145. Cladosporium gleditschiae (K 121559). A. Conidiophores arising from hyphal ropes. B. Detail of the scar on a conidium. Scale bars = 10 (A), 5 (B) µm.
In vitro: Mycelium mainly immersed, sparingly branched, 2−5
μm wide, septate, not constricted at septa, pale brown, smooth
to minutely verruculose, walls unthickened. Conidiophores
macro- and micronematous, solitary, arising terminally and
laterally from ascending or plagiotropous hyphae, erect, straight
to slightly flexuous, cylindrical-oblong to filiform, non-nodulose,
sometimes geniculate, unbranched to once branched, branches
as short denticle-like lateral outgrowth, later becoming longer,
17−165 × 3−5 μm, micronematous conidiophores (1−)2−2.5(−3)
μm wide, 0−4-septate, cells quite long, not constricted at septa,
septa often darkened, pale to pale medium brown, slightly
paler towards the apex, minutely verruculose, asperulate,
walls unthickened or slightly thickened, up to 1 μm wide.
Conidiogenous cells integrated, often distinctly sympodially
proliferating, terminal, usually non-nodulose, sometimes slightly
geniculate, filiform to cylindrical-oblong, somewhat flexuous,
17−55 μm long, with up to three apical loci, sitting close together
at the apex, conspicuous, subdenticulate to denticulate,
(1.2−)1.5−2(−2.2) μm diam, thickened and darkened-refractive.
Ramoconidia cylindrical-oblong, 19−41(−56) × 3−4(−5) μm,
0(−2)-septate, base broadly truncate. Conidia catenate, in
densely branched chains, straight to slightly curved, small
terminal conidia globose, subglobose to obovoid, 2.5−6(−8) ×
(2.5−)3−4 μm (av. ± SD: 4.5 ± 1.6 × 3.2 ± 0.5), broadly rounded
at the apex, intercalary conidia subglobose, broadly ellipsoidovoid, (4−)5−9(−14) × 3−4(−5) μm (av. ± SD: 6.6 ± 2.2 × 3.7
± 0.5), aseptate, with up to 3(−5) distal hila, often distinctly
denticulate, secondary ramoconidia ellipsoid to subcylindrical,
9−25(−30) × (3−)4−5(−6) μm (av. ± SD: 16.6 ± 5.6 × 4.3 ± 0.5),
0(−1)-septate, with 3−4 distal hila, sometimes hila not only distal
but also lateral in the middle of the cell, pale brown, smooth
or almost so, under SEM surface reticulate or with somewhat
embossed stripes caused by diminishing turgor and shriveling
of tender young conidia, walls unthickened or only slightly so,
attenuated towards apex and base, hila conspicuous, often
distinctly denticulate, 0.5−2 μm diam, thickened and darkenedrefractive; microcyclic conidiogenesis not observed.
Culture characteristics: Colonies on PDA grey-olivaceous to
olivaceous, reverse leaden-grey, velvety to powdery, margin
colourless to white, feathery, aerial mycelium sparse, loose, luffy,
only few areas covered, growth lat, without exudates, sporulation
profuse. Colonies on MEA grey-olivaceous, pale olivaceous-grey
towards margins, reverse olivaceous-grey, velvety, due to aerial
mycelium several white patches, luffy, loose to dense, without
140
Fig. 146. Cladosporium globisporum (CBS 812.96). Macro- and micronematous
conidiophores, ramoconidia and conidial chains in vitro. Scale bar = 10 µm. K.
Bensch del.
exudates, sporulation profuse. Colonies on OA grey-olivaceous
to pale olivaceous due to profuse sporulation or olivaceous-buff,
reverse leaden-grey to iron-grey, velvety to powdery, glittering due
to numerous small, not very prominent exudates (like little water
drops), margin colourless, feathery, aerial mycelium absent or
sparse, growth lat.
Substrate and distribution: Isolated from meat stamp; Sweden.
Notes: Cladosporium globisporum, which clustered in phylogenetic
analyses as a basal sister to C. phyllophilum (Bensch et al. 2010,
ig. 1, part a), is morphologically somewhat intermediate between
the C. cladosporioides and C. sphaerospermum complex. The
the genuS Cladosporium
Fig. 147. Cladosporium globisporum (CBS 812.96). A–F. Macronematous conidiophores and conidial chains. Scale bar = 10 µm.
conidiophores are rather C. cladosporioides-like, whereas the
terminal and intercalary globose or subglobose conidia are rather
reminiscent of C. sphaerospermum, although they are smooth and
not verruculose as in the latter species.
70. Cladosporium grech-delicatae Sacc., Ann. Mycol.
11: 564. 1913 [and Nuovo Giorn. Bot. Ital., N.S. 21(1): 125.
1914]. Fig. 149, 150.
Holotype: Malta, Uied Bufula, on still living stems of Ranunculus
aquatilis (Ranunculaceae), Apr. 1913, Doct. Borg (PAD).
Lit.: Saccardo (1931: 794), Schubert (2005b: 83–85).
Ill.: Schubert (2005b: 84, ig. 32, pl. 15, igs A–C).
In vivo: On still living stems, forming small oblong patches,
1–3 mm long, 1 mm wide, dark, blackish, sometimes confluent.
Colonies loose to dense, compact, pale to dark brown, villose
to somewhat floccose when well fruiting. Mycelium internal,
subcuticular to intraepidermal; hyphae sparsely branched, 3–8
μm wide, septate, sometimes slightly constricted at the septa,
www.studiesinmycology.org
pale to medium olivaceous-brown or somewhat darker at the
base of conidiophores, sometimes subhyaline, smooth, walls
slightly thickened, sometimes with small swellings, forming
loose to somewhat denser stromatic hyphal aggregations or
plates, occasionally with irregularly lobed cells. Conidiophores
solitary or in small loose groups, but not fasciculate, arising
from hyphae, swollen hyphal cells or from hyphal aggregations,
erumpent through the cuticle, erect to rarely subdecumbent,
straight or almost so to slightly flexuous, cylindrical-oblong to
filiform, usually not geniculate, non-nodulose, unbranched,
sometimes once branched, 40–235 μm long or even longer,
3.5–8(–9) μm wide, pluriseptate, sometimes slightly constricted
at the septa, medium to dark olivaceous-brown or brown,
walls thickened, often distinctly two-layered, walls up to 1 μm
wide, smooth to somewhat asperulate or irregularly roughwalled, slightly attenuated towards the apex, protoplasm of the
cells sometimes aggregated near the septa, appearing to be
somewhat thickened, similar to distoseptation. Conidiogenous
cells integrated, terminal, rarely intercalary, cylindrical-oblong,
14–43 μm long, proliferation sympodial, at the apex with few
subdenticulate conidiogenous loci, protuberant, truncate
141
BenSch et al.
Fig. 149. Cladosporium grech-delicatae (PAD). Conidiophores, ramoconidia and
conidia in vivo. Scale bar = 10 µm. K. Bensch del.
brown, smooth, walls somewhat thickened, lumen apparently
somewhat distinct, apex often obtuse, rounded or with a single
or few hila, hila truncate to slightly convex, slightly protuberant,
0.5–1.5(–2) μm diam, somewhat thickened and refractive.
Substrate and distribution: On Ranunculus aquatilis (≡ Batrachium
aquatile); Malta.
Notes: This species is comparable with C. cladosporioides but
distinct in having shorter and wider conidia, wider, thick-walled,
often distinctly two-layered conidiophores and sometimes
irregularly lobed hyphae.
Fig. 148. Cladosporium globisporum (CBS 812.96). A–B. Delicately ornamented
conidia showing a somewhat irregularly reticulate surface or slightly embossed
stripes probably caused by diminishing turgor and shriveling of tender young conidia.
C. Conidia, secondary ramoconidia and scars. D. Conidiophore with secondary
ramoconidia. E. Globose ornamented small terminal conidia. F. Running hyphae on
agar and conidia. Scale bars = 2 (B, E), 5 (A, C), 10 (D, F) µm.
or obconically truncate, 1.5–2(–2.5) μm diam, dome and
surrounding rim sometimes not very conspicuous, somewhat
thickened and darkened-refractive. Ramoconidia ellipsoid,
subcylindrical to cylindrical, 15–23 × 5–7.5 μm, 0(–1)-septate,
pale to medium brown or olivaceous-brown, with few apical
hila, base truncate, 2–3 μm wide, without dome and raised
rim, unthickened. Conidia catenate, usually in branched
chains, subglobose, obovoid, narrowly to broadly ellipsoid or
subcylindrical, (2.5–)5–17 × (2.5–)3.5–6 μm, 0–1-septate, not
constricted at the septa, pale to medium brown or olivaceous142
71. Cladosporium grevilleae Crous & Summerell, IMA
Fungus 2(1): 51. 2011. Figs 151, 152.
Holotype: Australia, New South Wales, Mount Annan Botanical
Garden, on leaves of Grevillea sp. (Proteaceae), Aug. 1999, P.W.
Crous & B.A. Summerell JT 974 (DAR 74881). Ex-type cultures:
CPC 2913–2916 = CBS 114271.
Ill.: Crous et al. (2011b: 63, igs 2–3).
In vivo: Leaf spots absent. Ascomata occurring in leaf litter,
amphigenous, black, subepidermal, erumpent to supericial,
globose, to 100 μm diam, with central, periphysate ostiole, 10–
15 μm diam; wall of 3–4 layers of brown textura angularis. Asci
aparaphysate, fasciculate, bitunicate with issitunicate discharge,
subsessile, obovoid to broadly ellipsoid, slightly curved, 8-spored,
35–45 × 9–12 μm, with visible apical apiculus. Ascospores tri- to
the genuS Cladosporium
Fig. 150. Cladosporium grech-delicatae (PAD). A. Overview. B. Tip of a conidiophore. C. Ramoconidium and conidia. Scale bars = 10 (B–C), 50 (A) µm.
Fig. 151. Cladosporium grevilleae (DAR 74881). Asci with ascospores. Ascospores showing sheath, and angular inclusions. Scale bar = 10 µm. P. Crous del.
Fig. 152. Cladosporium grevilleae (DAR 74881). A–B. Asci. C. Ostiolar region (arrowed). D. Ascus with ascospores. Scale bars = 10 µm.
www.studiesinmycology.org
143
BenSch et al.
multi-seriate, hyaline, guttulate with angular inclusions, thickwalled, straight to slightly curved, fusoid-ellipsoid with obtuse
ends, medianly 1-septate, widest in middle of apical cell, slightly
constricted at the septum, tapering towards both ends, but slightly
more to lower end, (9–)11–12(–13) × 3.5–4(–4.5) μm; ascospores
surrounded with a thin sheath when mounted in water, becoming
brown and verruculose with age; ascospore germination with
germ tubes parallel to the long axis of the spore, but distorting
prominently (original spore cells up to 8 μm wide), germinating
with numerous germ tubes, forming dense clusters of hyphae, but
mostly remaining hyaline after 24 h on MEA.
Culture characteristics: Colonies after 2 wk at 24 °C spreading,
reaching 15–20 mm diam. On MEA erumpent, with even, lobed
margins; surface folded, with sparse aerial mycelium, olivaceousgrey; reverse iron-grey. On OA lat, with sparse aerial mycelium,
and lobed, somewhat feathery margins; surface pale olivaceousgrey in middle, iron-grey in outer region. On PDA erumpent,
with lobed, feathery margins; surface folded, with sparse aerial
mycelium, grey-olivaceous; reverse iron-grey.
Substrate and distribution: On Grevillea; Australia.
Notes: Cladosporium grevilleae forms only the sexual state of
the life-cycle, i.e., its anamorph was neither observed in vivo nor
formed in vitro. The ascospores are typical of a Cladosporium
teleomorph, having thick walls, and angular inclusions (Aptroot
2006), becoming brown and verruculose with age. Although
four analysed loci supported the association of the species with
Cladosporium, it did not match any of the Cladosporium sequences
currently available on the GenBank nucleotide database (closest
match on ITS was Davidiella macrospora GenBank EU167591
with 95 % identity, on EF-1α was Cladosporium myrtacearum
GenBank HM148360 with 97 % identity and on ACT it was
Cladosporium iranicum GenBank HM148599 with 89 % identity).
(Crous et al. 2011b).
72. Cladosporium halotolerans Zalar, de Hoog & GundeCimerman, Stud. Mycol. 58: 172. 2007. Fig. 153.
Holotype: Namibia, isolated from hypersaline water of salterns, 1
Sep. 2000, N. Gunde-Cimerman, isolated by P. Zalar, 1 Oct. 2000
(CBS H-19734). Ex-type culture: EXF-572 = CBS 119416.
Lit.: Haubold et al. (1998a), Buzina et al. (2003), Meklin et al.
(2004).
Ill.: Zalar et al. (2007: 172, ig. 8).
In vitro: Mycelium party submerged, partly supericial; hyphae
sparingly branched, (1–)2–4 µm wide, pluriseptate, septa often
appearing somewhat darkened, usually not constricted, pale brown
or pale olivaceous-brown, almost smooth or minutely verruculose,
walls unthickened, without extracellular polysaccharide-like
material. Conidiophores micronematous to semimacronematous,
arising laterally and terminally from hyphae, erect, straight to
somewhat lexuous, narrowly cylindrical-oblong, occasionally
slightly geniculate, non-nodulose, micronematous conidiophores
iliform or only as short peg-like or denticle-like lateral outgrowths
of hyphae, usually unbranched, sometimes intercalary with short
lateral denticulate outgrowths just below a septum, 4–150(–300)
144
× 2–3.5(–5.5) µm, micronematous conidiophores 1–1.5 µm
wide, 0–3-septate, septa often appearing darkened, sometimes
pluriseptate with septa in short succession, especially towards
the apex, septa not constricted, pale olivaceous-brown, smooth to
minutely verruculose, walls unthickened or almost so, sometimes
forming ramoconidia and fragments. Conidiogenous cells
integrated, terminal or sometimes intercalary, or conidiophores
reduced to conidiogenous cells, cylindrical, 4–18 µm long, usually
neither geniculate nor nodulose, with a single or up to three
protuberant, subdenticulate or denticulate conidiogenous loci,
0.7–1.5(–2) µm diam, thickened and darkened. Ramoconidia 15–
37 × 2–2.5(–3) µm, with up to ive septa, base broadly truncate,
about 2 µm wide, slightly thickened and somewhat darkenedrefractive. Conidia catenate, in branched chains, conidial chains
branching in all directions, terminal chains with up to nine conidia,
small terminal conidia globose or subglobose, 2–5(–6) × 2–3(–5)
µm (av. ± SD: 3.3 ± 0.7 × 2.5 ± 0.4), aseptate, intercalary conidia
subglobose, ovoid or ellipsoid, 3.5–9 × (2–)2.5–3 µm (av. ± SD:
6.2 ± 1.5 × 2.8 ± 0.2), aseptate, pale to medium brown, minutely
verruculose or verruculose, secondary ramoconidia ellipsoid,
fusiform or cylindrical, 7–25(–31) × 2–3.5(–6.5) µm (av. ± SD:
16.2 ± 6.1 × 2.5 ± 0.4), 0–3(–4)-septate, mostly 1-septate, not
constricted at septa, septa often somewhat darkened, pale to
medium brown, almost smooth to minutely verruculose, walls
unthickened, slightly attenuated towards apex and base, with up
to four distal hila, hila protuberant, subdenticulate or denticulate,
0.5–1.5(–2) µm diam, thickened and darkened-refractive;
microcyclic conidiogenesis not occurring.
Culture characteristics: Colonies on PDA attaining 27–43 mm
diam after 14 d, olivaceous, grey olivaceous or olivaceous-grey,
reverse olivaceous-grey to leaden-grey or olivaceous-black,
velvety, powdery to felty-wooly, margins white, regular, glabrous
or feathery, aerial mycelium absent or sparse, growth lat with a
somewhat elevated colony centre, without prominent exudates,
sporulation profuse. Colonies on MEA attaining 18–44 mm diam
after 14 d, smoke-grey, pale olivaceous-grey or olivaceous-grey,
sometimes glaucous-grey at margin, reverse olivaceous-grey,
powdery to felty-wooly, margin colourless to white, glabrous or
feathery, colony centre furrowed, aerial mycelium felty, abundant,
covering most of the colony surface, sporulating. Colonies
on MEA + 5 % NaCl 24–48 mm diam, olive, furrowed, velvety,
with more pale, undulate margins, reverse dark green to black.
Colonies on OA reaching 29–40 mm diam after 14 d, smoke-grey
to grey-olivaceous or dark mouse-grey, reverse olivaceous or
olivaceous-grey, velvety to felty, luffy, margin white, somewhat
feathery, aerial mycelium sparse, diffuse or abundantly formed,
high, dense, whitish, growth lat with papillate surface, sporulation
profuse. Maximum tolerated salt concentration: Only 15 % of
tested strains develop colonies at 20 % NaCl after 7 d, whereas
after 14 d all cultures grow and sporulate. Cardinal temperatures:
No growth at 4 °C, optimum at 25 °C, maximum at 30 °C. No
growth at 37 °C (from Zalar et al. 2007).
Substrates and distribution: Saprobic, isolated from hypersaline
water in subtropical climates, indoor environments, Arctic ice
and biomats, contaminant in lesions of humans and animals,
plants, rock, conifer wood and window frame, from mycorrhizal
roots; probably circumglobal, Africa (Namibia, South Africa),
Arctics, Asia (India, Israel, Turkey), Australasia (New Zealand),
Europe (Belgium, Bosnia and Herzegovina, Denmark, Germany,
Italy, Russia, Slovenia, Spain, Sweden, Switzerland, UK), North
the genuS Cladosporium
Fig. 153. Cladosporium halotolerans (CBS 119416) (from Zalar et al. 2007). A–D. Colony surface grown on PDA (A), OA (B), MEA (C) and MEA plus 5 % NaCl (D) of strains
incubated for 14 d at 25 ºC in darkness. E–H. Habit of conidiophores. I. Conidiophore. J. Succession of secondary ramoconidia. K. Conidia. E–K. All from 7-d-old SNA slide
cultures. A–B, from EXF-572 (ex-type strain); C–D, from EXF-977; E, G, from EXF-972; F, from EXF-564; H, I, K, from EXF-1072; J, from dH 12862. Scale bars = 10 (I–K), 30
(H), 50 (F–G), 100 (E) µm.
America (USA) and Central and South America (Brazil, Dominican
Republic).
www.studiesinmycology.org
Additional specimens examined: Sine loco, isol. from Citrus, 2007, B. Anderson,
BA 1701 (CPC 14302). Arctic, isol. from ice (dH 13911 = EXF-2422). Bosnia and
Herzegovina, ston salterns (EXF-944). Brazil, culture contaminant (dH 12862 = EXF2533); Fortaleza, isol. from nuts, 18 Jul. 2005, U. Braun (CPC 12224). Denmark,
isol. from water, 2006, B. Anderson, V 2515 (CPC 13641); Bronshoy, isol. from indoor
environment, wall over a door frame, old water damage, 2000, B. Anderson, BA 1727
145
BenSch et al.
(CPC 14366). Dominican Republic, salterns (EXF-698); salt lake Enriquilio (EXF703). India, isol. from Musa sp. (Musaceae), 25 Oct. 2004, M. Arzanlou (CPC 11615).
Israel, Dead Sea, isol. from hypersaline water (EXF-1072). Namibia, salterns (EXF565, EXF-567, EXF-571). New Zealand, Auckland, Mount Albert, isol. from leaf litter,
20 Jun. 2005, C.F. Hill (CPC 12316, stored as C. sphaerospermum); Coromandel,
Hahai, isol. from leaf lesions on Ficus benjamina (Moraceae), 28 Sep. 2005, C.F. Hill,
Hill 1259 (CPC 12479). Russia, Moscow region, isol. from Aureobasidium caulivorum
(CBS 573.78). Slovenia, bathroom (EXF-972, EXF-977); Sečovlje salterns (EXF-228
= MZKI B-840; EXF-380; EXF-2372). South Africa, Bethal, isol. from thatch, 2002,
CAMS 000863 (CPC 13997); Durban, isol. from thatch, 2002, CAMS 000825 (CPC
13996). Spain, Santa Pola salterns (EXF-646). Turkey, isol. from brain (dH 12991 =
EXF-2535). UK, isol. from laboratory air (CBS 191.54).
Notes: This species, which according to Zalar et al. (2007) probably
prefers (hyper)osmotic ecological niches, is morphologically close
to C. sphaerospermum. Its distribution is insuficiently known, but
based on the recorded collections from various indoor habitats and
as a culture contaminant in different parts of the world (Zalar et al.
2007), C. halotolerans is possibly cosmopolitan.
73. Cladosporium haplophylli (Vasyag. & Tartenova) J.C.
David, Mycol. Pap. 172: 85. 1997. Fig. 154.
Basionym: Heterosporium haplophylli Vasyag. & Tartenova, Krypt.
Fl. Kazakhst. 8(2): 164. 1975.
Holotype: Kazakhstan, Chimkentskaya Oblast, southern KyzylKum desert, on Haplophyllum latifolium (Rutaceae), 10 Jun. 1957,
M.A. Tartenova (probably not preserved). Paratype: Kazakhstan,
Chimkentskaya Oblast, Talasskii Alatau, Zapovednik AksuDzhabagly, river Aksu, on Haplophyllum latifolium, 23 Jul. 1968,
M.P. Vasyagina (AA).
Ill.: David (1997: 87, ig. 20).
In vivo: Leaf spots amphigenous, elliptical-subcircular, often at
the tips of the leaves or marginal, 1–4(–5) mm wide, pale beige to
medium brown, occasionally with a narrow somewhat raised margin,
surrounded by a diffuse yellowish halo. Colonies amphigenous,
mainly hypophyllous, punctiform, scattered to dense, dark brown.
Mycelium immersed. Stromata lacking, only with single swollen
hyphal cells, 4–18 µm diam, or small aggregations of swollen
hyphal cells, 10–40 µm diam, brownish. Conidiophores solitary
or in small groups or fascicles, arising from single swollen hyphal
cells or cell aggregations, erumpent, erect, straight, subcylindrical
to usually narrowed towards the apex, barely to slightly geniculatesinuous, unbranched, 8–40 × 4–10 µm, aseptate, pale to medium
yellowish brown or brown, wall thin, ≤ 1 µm, smooth to faintly roughwalled; conidiophores usually reduced to conidiogenous cells, with
1–2(–3) conidiogenous loci, conspicuously coronate, 2–3 µm wide
and 1 µm high. Conidia solitary or in short chains, ramoconidia
lacking, ellipsoid-ovoid, obovoid, pyriform, subcylindrical, 15–30 ×
8–12(–13) µm, 0–2-septate, pale to medium olivaceous-brown or
brown, wall thin, ≤ 1 µm, densely verruculose or verrucose, ends
broadly rounded or somewhat attenuated, with a single coronate
hilum, 1–2.5 µm diam.
Substrates and distribution: On Haplophyllum spp. (Rutaceae),
Central Asia – H. acutifolium (Turkmenistan), H. latifolium
(Kazakhstan).
Additional specimen examined: Turkmenistan, Maryskaya Oblast, Badkhyzskii
Zapovednik, Urochishche Er-Oilan-Duz, on Haplophyllum acutifolium, 20 Apr. 1978,
V.A. Mel’nik (IMI 269572, LE).
146
Fig. 154. Cladosporium haplophylli (IMI 269572). Conidiophores and conidia in vivo.
Scale bar = 10 µm. U. Braun del.
Notes: This is a leaf-spotting phytopathogenic fungus, wellcharacterised by having very short, aseptate conidiophores, arising
from basal swollen hyphal cells, and large, above all rather broad,
densely verruculose-verrucose conidia.
74. Cladosporium heleophilum J.C. David, Mycol. Pap.
172: 80. 1997. Fig. 155.
Holotype: USA, Washington, Seattle, on Typha latifolia (Typhaceae),
26 Aug. 1912, E. Bartholomew, Bartholomew, Fungi Columb. 4407
(K). Isotypes: Bartholomew, Fungi Columb. 4407.
Ill.: David (1997: 62, ig. 17 J–L).
Exs.: Bartholomew, Fungi Columb. 4407; Krieger, Fungi Saxon.
Exs. 2091 p.p.
In vivo: Saprobic on dead leaves. Colonies effuse or forming
small, brown, punctiform to oblong speckles on the leaf surface
composed of conidiophore tufts, often arranged in a line alongside
furrows in the leaf. Mycelium immersed; hyphae forming loose
stromatic aggregations, sometimes erumpent through the
cuticle. Conidiophores usually fasciculate, occasionally solitary,
erect, straight, subcylindrical to moderately geniculate-sinuous,
unbranched, 30–120 × 4–6 µm, septate, pale olivaceous-brown
to medium brown, sometimes paler towards the apex, wall thin to
slightly thickened (≤ 1 µm), smooth or almost so, cells occasionally
with distinct lumen, protoplasm aggregated at the septa, which
appear to be thickened. Conidiogenous cells integrated, terminal
and intercalary, sympodially proliferating, slightly to moderately
geniculate-sinuous, 10–30 µm long, usually with 1–4 distinctly
coronate conidiogenous loci, somewhat protuberant, 1.5–2 µm
diam. Conidia in simple or branched chains, elongate-ellipsoid to
cylindrical, short conidia sometimes ellipsoid-ovoid, 8–30(–35) ×
(3–)4–6(–7) µm, 0–3(–5)-septate, usually without any constrictions,
septate conidia and secondary ramoconidia 12–30 µm long, pale
olivaceous to olivaceous-brown, thin-walled, almost smooth to
usually verruculose, ends obtuse to slightly attenuated, hila 1–2 µm
diam, distinctly coronate, slightly protuberant, somewhat thickened
and darkened-refractive; microcyclic conidiogenesis not observed.
Substrates and distribution: On dead leaves of Typha latifolia
(Typhaceae) – Europe (Germany, Latvia, UK), North America
(USA, WA).
the genuS Cladosporium
A collection on necrotic patches on Rosa sp. (Brazil, State
of Ceará, São Benedito City, 30 Oct. 2001, F. Freire, HAL)
agrees morphologically well with C. heleophilum [conidiophores
fasciculate, 20–70 × 2–6 µm, geniculate; conidia in branched
chains, obovoid, ellipsoid to cylindrical, (6–)10–25 × 3–6(–7) µm,
0–3-septate, almost smooth to verruculose].
75. Cladosporium heliotropii Erikss., Bot. Centralbl. 47:
299, 1891 and Fungi Paras. Scand. Exs., Fasc. 8, No. 396.
1891. Figs 156–158.
Lectotype (designated here): Sweden, Stockholm, Rosendal,
on leaves of Heliotropium arborescens (= H. peruvianum)
(Boraginaceae), 1882, J. Eriksson, Erikss., Fungi Paras. Scand.
Exs. 396 (BPI 426853). Isolectotypes: Erikss., Fungi Paras. Scand.
Exs. 396 (e.g. HAL, HBG).
Lit.: Saccardo (1892: 602), Ferraris (1914: 884), Schubert (2005b:
85–87).
Ill.: Schubert (2005b: 86–87, igs 33–34, pl. 16, igs A–F).
Exs.: Eriksson, Fungi Paras. Scand. Exs. 396.
Fig. 155. Cladosporium heleophilum (K). Fascicle of conidiophores, conidiogenous
cells and conidia in vivo. Scale bar = 10 µm. U. Braun del.
Additional specimens examined: Germany, Mittelfranken, on Typha sp., 11 Oct.
1902, Zahn (HBG); Saxony, Ostrau near Schandau, on Typha latifolia, Jul. 1901/
Oct. 1904, Krieger, Fungi Saxon. Exs. 2091 (HAL, as Cladosporium typharum
mixed with C. macrocarpum); ex “Station für Planzenschutz zu Hamburg”, on Typha
latifolia, June 1903, without exact locality and collector (HBG). Latvia, Vidzeme,
Vestiena, on Typha latifolia, 20 Jul. 1933, K. Starcs (M-0057703).
Notes: This species, which is only known from a few herbarium
samples, is insuficiently known. It is undoubtely a saprobic
fungus. The size of the conidiophores and, above all, the width of
the conidia remind one of Cladosporium allicinum, but the latter
species differs from C. heleophilum in having distinctly nodulose
conidiophores. Cladosporium heleophilum belongs to a group of C.
herbarum-like species, but it is distinguished from the C. herbarum
complex by their non-nodulose conidiophores. Within this group of
species, C. heleophilum resembles C. antarcticum, isolated from
a lichen, and the saprobic C. subtilissimum. The latter species
is, however, distinct by having numerous small, usually narrowly
obovoid, limoniform to fusiform conidia and somewhat narrower,
usually 0–1-septate secondary ramoconidia. The conidiophores
of C. antarcticum are usually distinctly geniculate, with numerous,
often crowded, denticle-like conidiogenous loci. The differentiation
between C. heleophilum and allied species, above all C.
subtilissimum, is rather dificult and only tentative, since David’s
species is not yet known in vitro.
www.studiesinmycology.org
In vivo: On living leaves, forming pale to dark brown, dense patches.
Colonies epiphyllous, punctiform, scattered, caespitose, pale
brown to olivaceous-brown, loccose-villose. Mycelium external,
supericial; hyphae branched, septate, at irst 3–6 µm wide, later
distinctly swollen and often constricted at the septa, up to 10 µm
wide or even wider, almost subhyaline or pale olivaceous, later pale
olivaceous to medium brown or somewhat reddish brown, smooth
or almost so to irregularly rough-walled, rugose, walls slightly to
distinctly thickened, sometimes even two-layered, protoplasm
of the cells often aggregated at the septa, olivaceous-yellowish,
refractive and somewhat granular, surrounding walls much paler,
sometimes almost hyaline, sometimes with distinct lumen in the
centre, hyphae aggregated, forming loose to dense supericial,
stromatic hyphal aggregations. Stromata lacking. Conidiophores
solitary, arising from external, creeping hyphae, swollen hyphal
cells or stromatic hyphal aggregations, lateral or terminal, often
also formed secondarily (microcyclic conidiogenesis), arising from
swollen conidia, erect, straight to lexuous, narrowly cylindricaloblong or iliform, sometimes growing like and confusable
with hyphae, usually not geniculate, sometimes subnodulose,
unbranched or branched, 5–150(–210) × (2–)3–6 µm, septate,
sometimes slightly constricted at the septa, almost hyaline,
subhyaline to pale olivaceous-brown, smooth or almost so,
sometimes asperulate, walls somewhat thickened, cell structure
similar to hyphae, protoplasm distinct, clearly delineated from the
inner wall, sometimes with distinct, irregular lumen in the centre,
sometimes appearing to be distoseptate. Conidiogenous cells
integrated, mostly terminal, occasionally intercalary, cylindrical,
8–32 µm long, proliferation sympodial, with 1–3 conidiogenous loci,
often near the apex, sometimes situated on small lateral shoulders
or unilateral swellings, protuberant, subdenticulate, 1–2 µm diam,
thickened, refractive to somewhat darkened. Conidia catenate, in
unbranched or branched chains, subglobose, obovoid, ellipsoid,
fusiform to subcylindrical, occasionally cylindrical, 4–20(–25) × 3–7
µm wide, 0–1(–3)-septate, sometimes slightly constricted at the
septa, pale olivaceous or pale olivaceous-brown, smooth or almost
so to minutely verruculose, walls more or less thickened, with age
becoming distinctly swollen, longer and wider, up to 12 µm or even
147
BenSch et al.
Fig. 156. Cladosporium heliotropii (BPI 426853). Conidiophores and conidia in vivo. Scale bar = 10 µm. K. Bensch del.
wider, pluriseptate, often constricted at septa, sometimes with a
single longitudinal septum or a single distoseptum, thick-walled,
sometimes even two-layered, smooth to verruculose, sometimes
confusable and only barely distinguishable from swollen hyphae,
cell structure similar to hyphae and conidiophores, hila protuberant,
1–2 µm diam, thickened, refractive to somewhat darkened;
microcyclic conidiogenesis often occurring.
Substrates and distribution: On Heliotropium spp. (Boraginaceae);
Europe, North America – Heliotropium arborescens (Sweden; USA,
AK), Heliotropium sp. (USA, AK).
Additional specimens examined: USA, Alaska, Sitka, on Heliotropium sp., 1 Nov.
1914, J.P. Anderson, Alaska Fungi 144 (BPI 426850, BPI 426851) and Alaska Fungi
159 (BPI 426849, BPI 426852).
Notes: Some collections on Heliotropium sp. from Alaska housed
at BPI were examined and proved to be conspeciic with C.
heliotropii. They agree well with the latter species in forming an
external mycelium giving rise to solitary conidiophores, having
conidiophores and conidia with agreeing morphology and
showing the same cell structures as described above. They vary
in having longer conidiophores (up to 210 µm long), longer and
more frequently septate [0–3(–5) septa] conidia and occasionally
somewhat wider conidiogenous loci and hila (up to 3 µm diam).
Old, swollen conidia could only occasionally be observed, whereas
in the type material they were common. Ferraris (1914) recorded
148
Fig. 157. Cladosporium heliotropii (BPI 426851). Conidiophores and conidia in vivo.
Scale bar = 10 µm. K. Bensch del.
and described a single collection from Italy (Italy, near Turin, in a
garden, on leaves of Heliotropium peruvianum, P. Voglino) indicating
that C. heliotropii is probably more common and widespread than
the genuS Cladosporium
Fig. 158. Cladosporium heliotropii (BPI 426853 and HBG). A, D. Conidiophores with still attached conidia. B. Conidiophore arising from supericially growing hyphae. C, E.
Conidiophores and old swollen conidia. F. Small conidia. Scale bars = 10 (A–F) µm.
given under “Substrate and distribution”. Furthermore, he stated
that the conidia rapidly germinated in water, so that infections of
leaves above all occurred under humid conditions. French (1989)
reported this species from California, USA.
The phenomenon that conidia are become distinctly swollen,
larger and wider, pluriseptate and thick-walled with age, giving rise
to secondary conidiophores, is also known from C. dracaenatum,
but this species differs from C. heliotropii in having fasciculate,
mildly to distinctly geniculate-sinuous conidiophores arising from
true, substomatal to intra-epidermal stromata, and cells with distinct
lumina, as described above, were not observed.
www.studiesinmycology.org
76. Cladosporium herbaroides K. Schub., Zalar, Crous &
U. Braun, Stud. Mycol. 58: 120. 2007. Figs 159–161.
Holotype: Israel, Eilat salterns, isolated from hypersaline water,
2004, N. Gunde-Cimerman, isol. M. Ota (CBS H-19858). Isotype:
HAL 2025 F. Ex-type culture: CBS 121626 = EXF-1733 = CPC 12052.
Ill.: Schubert et al. (2007b: 121–123, igs 13–15).
In vitro: Mycelium branched, (1–)2–8 μm wide, septate, often with
small swellings and constrictions, subhyaline to pale brown or pale
149
BenSch et al.
Fig. 159. Cladosporium herbaroides (CPC 12052). Macro- and micronematous
conidiophores and conidia in vitro. Scale bar = 10 µm. K. Bensch del.
olivaceous-brown, smooth or almost so to somewhat verruculose,
walls unthickened or almost so. Conidiophores macronematous
and micronematous, arising lateral from plagiotropous hyphae or
terminally from ascending hyphae. Macronematous conidiophores
erect, straight to slightly lexuous, often geniculate, nodulose, with
unilateral or multilateral swellings, often numerous swellings in
short succession giving them a gnarled appearance, often forming
somewhat protruding or prolonged lateral swellings or a branchlike prolongation below the terminal swelling (due to sympodial
proliferation), unbranched or sometimes branched, 30–230 μm
long or even longer, 3–5 μm wide, swellings 5–8 μm wide, septate,
not constricted at septa, pale to medium olivaceous-brown,
smooth or almost so, walls slightly thickened. Conidiogenous cells
integrated, terminal or intercalary, cylindrical, usually nodulose to
nodose forming distinct swellings, sometimes geniculate, 15–55
μm long, with numerous conidiogenous loci usually conined to
swellings or situated on small lateral shoulders, sometimes on the
top of short peg-like prolongations or denticles, loci protuberant,
1–2 μm diam, thickened and darkened-refractive. Micronematous
conidiophores much shorter, narrower, paler, neither nodulose nor
geniculate, arising laterally from plagiotropous hyphae, often only
as short lateral denticles or branchlets of hyphae, erect, straight,
conical to cylindrical, unbranched, 3–65 × 2–3 μm, mostly aseptate,
Fig. 160. Cladosporium herbaroides (CPC 12052). A–B, D. Macronematous conidiophores. C. Conidial chain. E. Micronematous conidiophore. F. Microcyclic conidiogenesis.
G. Conidia formed by micronematous conidiophores. Scale bars = 10 µm.
150
the genuS Cladosporium
Fig. 161. Cladosporium herbaroides (CPC 12052). A. Overview of the growth characteristics of this fungus. Broad hyphae run over the surface of the agar, and possibly give rise
to conidiophore branches. The conidiophores of this fungus can be rather long, resembling aerial hyphae. Clusters of conidia are clearly visible in this micrograph. B. The very
wide surface hyphae can anastomose. C. Conidiophore with secondary ramoconidia and conidia. Note the variation in scar size. D. A very elaborate, complex conidiophore with
different scars of variable size, one being more than 2 μm wide! E. Details of secondary ramoconidia and hila. Note the rather strong ornamentation in which smaller “particles”
are between larger ones. F. Three conidia in a row. Note the scar formation in the chain and the reduction of the size of the cells throughout the spore-chain. The inset shows
the resemblance of the scars on a conidiophore and on a secondary ramoconidium. Scale bars = 2 (F), 5 (D–E), 10 [B–C, F (inset)], 50 (A) µm.
sometimes up to ive septa, subhyaline, smooth, walls unthickened.
Conidiogenous cells integrated, terminal or conidiophores reduced
to conidiogenous cells, conidiogenous loci solitary or sometimes
as sympodial clusters of pronounced denticles, protuberant, 1–1.5
μm diam, thickened and somewhat darkened-refractive. Conidia
polymorphous, two main morphological types recognisable, formed
by the two different types of conidiophores, conidia formed by
macronematous conidiophores catenate, in branched chains,
straight to slightly curved, subglobose, obovoid, limoniform,
ellipsoid to cylindrical, 3–33 × (2–)3–6(–7) μm [av. ± SD, 14.5
www.studiesinmycology.org
(± 7.9) × 5.2 (± 1.2) μm], 0–2(–3)-septate, sometimes slightly
constricted at septa, septa median or somewhat in the lower
half, pale to medium olivaceous-brown, verruculose to verrucose
(granulate under SEM), walls slightly thickened, with up to three
rarely four distal scars, with age becoming medium or even dark
brown (chocolate brown), wider and more thick-walled, 5.5–33 ×
(3.5–)5–9(–11) μm [av. ± SD, 14.4 (± 6.9) × 7.2 (± 1.9) μm], walls up
to 1 μm thick, hila protuberant, 0.8–2(–2.5) μm diam, thickened and
darkenedrefractive; microcyclic conidiogenesis occurring. Conidia
formed by micronematous conidiophores paler and narrower,
151
BenSch et al.
mostly formed in unbranched chains, sometimes in branched chains
with up to three distal hila, straight to slightly curved, limoniform,
narrowly fusiform, almost iliform to subcylindrical, 10–26(–35) ×
2–3.5 μm [av. ± SD, 15.6 (± 6.2) × 2.9 (± 0.5) μm], 0–1(–3)-septate,
subhyaline to pale brown, almost smooth to minutely verruculose,
walls unthickened, hila protuberant, 1–1.5 μm diam, thickened and
somewhat darkened-refractive.
Culture characteristics: Colonies on PDA attaining 23 mm diam
after 14 d at 25 ºC, grey-olivaceous to olivaceous, olivaceous-grey
reverse, velvety, margin regular, entire edge, narrow, feathery, aerial
mycelium abundantly formed, loose, with age covering large parts
of the colony, woolly, growth lat with somewhat elevated colony
centre, folded, regular, deep into the agar, with few prominent
exudates, sporulation profuse. Colonies on MEA attaining 24 mm
diam after 14 d at 25 ºC, grey- to greenish olivaceous, olivaceousgrey or iron-grey reverse, velvety to powdery, margin narrow,
colourless, entire edge, somewhat feathery, aerial mycelium pale
olivaceous-grey, sparse, growth convex, radially furrowed, folded
in the colony centre, without prominent exudates, sporulating.
Colonies on OA attaining 23 mm diam after 14 d at 25 ºC, greyolivaceous, margin more or less regular, entire edge, colourless,
somewhat feathery, aerial mycelium whitish to smoke grey, at
irst sparse, later more abundantly formed, growth lat, without
exudates, sporulation profuse.
Substrates and distribution: Isolated from hypersaline water; Israel,
but probably more widespread (see “notes”).
Notes: Cladosporium herbaroides belongs to the C. herbarum
complex but is distinguished by having somewhat longer conidia
becoming wider, darker and even more thick-walled with age [at irst
conidia 3–33 × (2–)3–6(–7) μm, with age (3.5–)5–9(–11) μm wide]
and by forming a second conidial type formed on micronematous
conidiophores, giving rise to unbranched conidial chains which
are almost iliform, limoniform, narrowly fusiform to subcylindrical,
much narrower and paler than the ones formed by macronematous
conidiophores, 10–26(–35) × 2–3.5 μm (Schubert et al. 2007b).
Two old herbarium collections morphologically well agreeing
with C. herbaroides have been examined [Germany, Hannover,
Nordstemmen, on old leaves of Populus sp., 12 Sep. 1883,
Eichelbaum (HBG); Germany, Bavaria, Fränkische Schweiz,
Jura, Hirschbach, on necrotic leaf spots caused by a coelomycete
(Phyllosticta or Septoria), on Rhamnus cathartica, Sep. 1904, Zahn
(HBG)].
Fig. 162. Cladosporium herbarum (RO, holotype of the teleomorphic state Davidiella
tassiana). Ascus and ascospores in vivo. Scale bar = 10 µm. P.W. Crous del.
77. Cladosporium herbarum (Pers. : Fr.) Link, Ges. Naturf.
Freunde Berlin Mag. Neuesten Entdeck. Gesammten Naturk.
7: 37. 1816: Fr., Syst. mycol. 3(2): 370. 1832. Figs 162–165.
Basionym: Dematium herbarum Pers., Ann. Bot. (Usteri) 11: 32.
1794 : Fr., Syst. mycol. 3(2): 370. 1832.
≡ Acladium herbarum (Pers.) Link, Ges. Naturf. Freunde Berlin Mag.
Neuesten Entdeck. Gesammten Naturk. 3: 12. 1809.
≡ Byssus herbarum (Pers.) DC., Fl. franç., Ed. 3, 5: 11. 1815, as “Bissus”.
≡ Dematium vulgare Pers., Mycol. eur. 1: 13. 1822, nom. superl.
≡ Dematium vulgare a herbarum (Pers.) Pers., Mycol. eur. 1: 13. 1822.
Lectotype (selected by Prasil & de Hoog 1988): Sine loco, sine
dato, ex herb. Persoon (L 910.225-733). Epitype (designated by
Schubert et al. 2007b): Netherlands, Wageningen, isolated from
Hordeum vulgare (Poaceae), 2005, P.W. Crous (CBS H-19853).
152
Fig. 163. Cladosporium herbarum (CPC 11600). Macro- and micronematous
conidiophores and conidia in vitro. Scale bar = 10 µm. K. Bensch del.
Isoepitype: HAL 2022 F. Ex-epitype culture: CBS 121621 = CPC
12177–12179, 12181, 12183.
the genuS Cladosporium
Fig. 164. Cladosporium herbarum (CPC 11600), anamorphic and teleomorphic states (from the host and CPC 12181). A–B. Macronematous conidiophores. C. Micronematous
conidiophore. D. Microcyclic conidiogenesis. E. Conidial chain. F. Ascomata on the leaf. G. Ascomata formed in culture on nettle stems. H–I. Asci on the host. J–K. Ascospores
in culture. L. Asci in culture. Scale bars = 10 (A, E, H, J–L), 200 (F–G, I) µm.
= Dematium epiphyllum Pers., Syn. meth. fung. 2: 695. 1801 [type: L 910.225646].
≡ Cladosporium epiphyllum (Pers.) Nees, Syst. Pilze 1: 67. 1817.
≡ Cladosporium epiphyllum (Pers.) Link, in Willd., Sp. pl. 6(1): 42. 1824.
≡ Chloridium epiphyllum (Pers.) Chevall., Fl. gén. env. Paris 1: 35. 1826.
≡ Cladosporium epiphyllum (Pers.) Fr., Syst. mycol. 3(2): 370. 1832.
= Dematium herbarum γ fungorum Pers., Syn. meth. fung. 2: 699. 1801 [type:
L 910.225-732].
≡ Dematium vulgare γ fungorum (Pers.) Pers., Mycol. eur. 1: 14. 1822.
≡ Cladosporium herbarum β fungorum (Pers.) Chevall., Fl. gén. env.
Paris 1: 36. 1826.
≡ Cladosporium fungorum (Pers.) Roum., Fungi Sel. Gall. Exs., Cent. 33,
No. 3293. 1885, as “Pers.”.
= Dematium epiphyllum β chionanthi Pers., Mycol. eur. 1: 16. 1822 [holotype:
L 910.255-872 = L 0115833].
≡ Cladosporium epiphyllum β chionanthi (Pers.) Link, in Willd., Sp. pl.
6(1): 42. 1824.
= Dematium fuscum Pers., Mycol. eur. 1: 16. 1822 [type: L 910.225-720].
≡ Cladosporium fuscatum Link, in Willd., Sp. pl. 6(1): 41. 1824, non C.
fuscum Link, 1824.
= Botrytis pulvinata Link, in Willd., Sp. pl. 6(1): 61. 1824 [holotype: B
700006764].
= Cladosporium typharum Desm., Pl. Crypt. N. France, Ed. 1, Ser. 2, Fasc. VII,
www.studiesinmycology.org
No. 304. 1828 [syntypes: e.g., K, PC].
= Cladosporium sparsum Schwein., Trans. Amer. Philos. Soc., N.S., 4(2): 277.
1832 [syntypes: PH 1020413-1020414].
= Cladosporium typhae Schwein., Trans. Amer. Philos. Soc., N.S., 4(2): 277.
1832 [type: PH].
= Helminthosporium (“Helmisporium”) herbarum Schwein., Trans. Amer.
Philos. Soc., N.S. 4(2): 279. 1832 [type: PH].
= Acladium heterosporum Wallr., Flora crypt. Germ. 2: 287. 1833 [type: STR].
= Cladosporium alnicola Corda, Icon. fung. 1: 14. 1837 [type: PRM].
≡ Didymotrichum alnicola (Corda) Bonord., Handb. Mykol.: 89. 1851.
= Cladosporium caricicola Corda, Icon. fung. 1: 14. 1837 [type: PRM].
≡ Didymotrichum caricicola (Corda) Bonord., Handb. Mykol.: 89. 1851.
= Cladosporium graminum Corda, Icon. fung. 1: 14. 1837, nom. illeg., non C.
graminum (Pers.) Link, 1824.
= Cladosporium fasciculatum Corda, Icon. fung. 1: 15. 1837 [type: PRM].
= Cladosporium lignicola Corda, Icon. fung. 1: 14. 1837 [type: PRM 155424].
= Cladosporium nodulosum Corda, Icon. fung. 1: 15. 1837 [type: PRM].
≡ Didymotrichum nodulosum (Corda) Bonord., Handb. Mykol.: 89. 1851.
= Cladosporium tomentosum Corda, Icon. fung. 1: 15. 1837 [type: PRM].
= Helminthosporium (“Helmisporium”) lexuosum Corda, Icon. fung. 1: 13.
1837 [T: PRM].
≡ Brachysporium lexuosum (Corda) Sacc., Syll. fung. 4: 429. 1886.
= Myxocladium arundinis Corda, Icon. fung. 1: 12. 1837 [type: PRM 155582].
153
BenSch et al.
Fig. 165. Cladosporium herbarum (CPC 11600). A. Overview of hyphal growth and conidiophore formation of a colony on SNA. Conidiophores are often formed on very wide
(approx. 10 µm), septate hyphae that often grow near the agar surface. B. A more detailed view on colony organisation reveals the ornamented conidia. Note the septum near
the conidiophore (arrow). C. Detail of spore ornamentation and hila on a secondary ramoconidium (arrow). Ornamentation is visible during early stages of spore formation
(arrow). D. Structure of the conidiophore, illustrating the complex morphology of the spore-forming apparatus. In addition, secondary ramoconidia, conidia, and a hilum on
the conidium are visible. E. Complex structure of the spore-forming apparatus. F. Details of secondary ramoconidia with complex scar-pattern on the right cell. G. Details of a
secondary ramoconidium giving rise to conidia. Note the lack of ornamentation at the location of spore formation. Scale bars = 5 (C–E, G), 10 (B, F), 50 (A) µm.
≡ Cladosporium arundinis (Corda) Sacc., Syll. fung. 4: 364. 1886.
= ? Cladosporium herbarum var. typharum Westend. & Van Haes., Catalogue
des Cryptogames observes depuis 1835, dans le Brabant et das la Province
d’Anvers: 8, no. 173. 1838.
= Cladosporium amaranticola Opiz, Lotos 5: 41. 1855, nom. nud. [type: PRM].
= Cladosporium caespiticium Rabenh., Fungi Eur. Exs., Cent. VI, No. 579.
1863 and Bot. Zeitung (Berlin) 21: 230. 1863, nom. nud. [lectotype: M-0057455;
isolectotypes: HBG, HAL]
= Sphaerella tassiana De Not., Sferiacei Italici 1: 87. 1863.
≡ Mycosphaerella tassiana (De Not.) Johanson, Öfvers. Förh. Kongl.
Svenska Vetensk.-Akad. 41: 167. 1884.
≡ Davidiella tassiana (De Not.) Crous & U. Braun, Mycol. Progr. 2: 8.
2003.
= Cladosporium fuligineum Bonord., Abh. Mykol. 1: 92. 1864 [neotype: B,
154
selected by Braun 2001].
= Cladosporium microporum Rabenh., in Marcucci, Unio Itin. Crypt., No. 42.
1866, nom. nud.
≡ Cladosporium microporum Rabenh., in Cooke, Grevillea 17(83): 66.
1889 [syntypes: HBG, M-0057685].
= Cladosporium herbarum f. napi Thüm., Herb. Mycol. Oecon., Fasc. III, No.
107. 1873 [syntype: M].
= Cladosporium herbarum f. Holci mollis Thüm., Herb. Mycol. Oecon., Fasc.
IV, No. 161. 1874 [syntype: M].
= Cladosporium herbarum f. Tritici vulgaris Thüm., Herb. Mycol. Oecon., Fasc.
IV, No. 204. 1874 [syntype: M].
= Cladosporium herbarum var. torulosum Berk. & Broome, The Fungi of
Ceylon, no. 886. 1870; J. Linn. Soc., Bot. 14: 99. “1873” 1875 [type: K].
= Cladosporium herbarum f. phaseoli Thüm., Fungi Austr. Exs. 1293. 1875
the genuS Cladosporium
[syntypes: e.g. BPI 427201, HAL].
= Helminthosporium vesiculosum Thüm., Mycoth. Univ., Cent. VIII, No. 784.
1877 [syntypes: e.g. B, HAL, PAD].
≡ Brachysporium vesiculosum (Thüm.) Sacc., Syll. fung. 4: 429. 1886.
= Cladosporium herbarum f. Brassicae Botrytis Thüm., Herb. Mycol. Oecon.,
Fasc. XIII, No. 613. 1878 [syntype: M].
= Cladosporium herbarum f. Foeniculi oficinalis Thüm., Mycoth. Univ., Cent.
10, No. 981. 1878 [syntypes: HAL, M].
= Cladosporium elegans Penz., in Saccardo, Michelia 2(8): 471. 1882
[neotype: PAD (Saccardo, Mycoth. Ital. 1189)].
= Cladosporium punctulatum Sacc. & Ellis, Michelia 2(8): 578. 1882 [lectotype:
NY; isolectotypes: BPI 427402, PAD].
= Cladosporium macrocarpum f. fraxini Roum., Fungi Sel. Gall. Exs., Cent. 22,
No. 2158. 1882 [syntype: B].
= Cladosporium profusum f. robustior Roum., Fungi Sel. Gall. Exs., Cent. 24,
No. 2364. 1883 [lectotype (designated here): Roumeguère, Fungi Sel. Gall.
Exs. 2364 (B 700006687)].
≡ Cladosporium profusum var. robustior Roum. & Pat., Rev. Mycol.
(Toulouse) 5: tab. 35. ig. 6. 1883.
= Cladosporium brunneum Cooke & Harkn., Grevillea 12: 96. 1884, nom. illeg.,
non C. brunneum Corda, 1837 [holotype: K 121546; isotype: BPI 426168].
≡ Cladosporium brunneolum Sacc., Syll. fung. 4: 358. 1886.
= Helminthosporium phyllophilum P. Karst., Hedwigia 23: 41. 1884 [type: H].
= Heterosporium abroniae Harkn., Bull. Calif. Acad. Sci. 1: 38. 1884 [type:
CAS 2558, now BPI 1108748].
= Helminthosporium nanum f. petiolicola Roum., Fungi Sel. Gall. Exs., Cent.
34, No. 3391. 1885 [syntypes: G, PC].
= Heterosporium epimyces Cooke & Massee, in Cooke, Grevillea 16(79): 80.
1888 [type material not preserved; neotype (designated here): UK, Millield,
on Polyporus squamosus, 14 Oct. 1864, ex herb. M.C. Cooke, K (M) 121555].
= Heterosporium laburni Oudem., Ned. Kruidk. Arch., Ser. 2, 5(2): 174. 1888
[holotype: L].
= Cladosporium condylonema Pass., in Briosi & Cavara, Fung. Paras. Piante
Colt. Utili Ess., No. 79. 1889 [syntypes: e.g., BPI 426388, HAL].
= Heterosporium goiranicum C. Massal., Nuovo Giorn. Bot. Ital. 21: 170. 1889
and Mem. Accad. Agric. Verona, Ser. III, Fasc. 2, 65: 117. 1889 [holotype: VER].
= Cladosporium velutinum Ellis & Tracy, J. Mycol. 6: 76. 1890 [holotype: NY].
= Helminthosporium acuum P. Karst., Hedwigia 31: 295. 1892 [type: H].
= Helminthosporium compactum P. Karst., Hedwigia 31: 295. 1892 [type: H].
= Heterosporium galii Fautrey & Roum., Rev. Mycol. (Toulouse) 14: 106. 1892
[lectotype: PC].
= Heterosporium caulicola Ellis & Everh., Proc. Acad. Nat. Sci. Philadelphia
46: 381. 1894, as “caulicolum” [holotype: NY].
= Heterosporium cladosporioides Ellis & Everh., Proc. Acad. Nat. Sci.
Philadelphia 46: 382. 1894 [holotype: NY].
= Heterosporium didymosporum Clem., Bot. Surv. Nebraska 3, 1893(2): 11.
1894 [holotype: NEB].
= Cladosporium acutum Ellis & Dearn., Proc. Canad. Inst., N.S., 3, 1: 91. 1897
[syntypes: DAOM, NYS].
= Cladosporium fusicladium Sacc., in Bresadola & Saccardo, Malpighia 11:
321. 1897 [holotype: PAD].
= Cladosporium herbarum var. vincetoxici Allesch., in P. Sydow, Hedwigia
36(6), Beibl.: 163. 1897 [T: M].
= Heterosporium avenae Oudem., Hedwigia 37: 318. 1898 [lectotype
(designated by David 1997): L].
= Heterosporium syringae Oudem., Hedwigia 37: 183. 1898 [lectotype
(designated by David 1997): L].
= ? Cladosporium fagi Oudem., Ned. Kruidk. Arch., Ser. 3, 2(3): 768. 1902 and
Beih. Bot. Centralbl. 11: 538. 1902.
= Cladosporium herbarum f. dianthi D. Sacc., Mycoth. Ital. No. 1385. 1904?
(syntypes: e.g., BPI 427194, SIENA).
= Cladosporium laricis Sacc., Ann. Mycol. 3: 515. 1905 [holotype: PAD].
= Cladosporium graminum var. moliniae-caeruleae Sacc., Ann. Mycol. 3: 169.
1905 [syntypes: Saccardo, Mycoth. Ital. 1583, e.g., SIENA].
= Heterosporium ephedrae Potebnia, Ann. Mycol. 5: 21. 1907 and Trudy
Oshch. Isp. Prir. Imp. Khar’kovsk. Univ. (Trudy Oshch. Estest. imp. Khar’kov
Univ.) 1907: 43. 1907 [lectotype (designated by David 1997): PAD].
= Heterosporium fraxini Ferd. & Winge, Bot. Tidsskr. 28(2): 256. 1907
[holotype: C].
= Heterosporium opuntiae Lindau, in Rabenhorst, Krypt.-Fl., ed. 2, 1(9): 84.
1910 [holotype: B].
= Heterosporium berberidis Ranoj., Ann. Mycol. 8: 399. 1910 [type: BPI
1108749].
= Heterosporium cytisi Ranoj., Ann. Mycol. 8: 398. 1910 [type: BPI 1108761].
= Cladosporium vincae Fairm., Ann. Mycol. 9: 148. 1911 [holotype: CUP-F
2873(24-68)].
= Heterosporium asperatum Massee ex Sacc., Syll. fung. 22: 1388. 1913.
www.studiesinmycology.org
= Heterosporium spiraeae Syd. & P. Syd., Ann. Mycol. 11: 406. 1913 [holotype:
S; isotype: IMI 16609].
= Heterosporium yuccae Bubák, Ann. Mycol. 12: 214. 1914 [lectotype
(designated by David 1997): BPI 802198].
= Heterosporium sorghi Ranoj., Ann. Mycol. 12: 418. 1914 [holotype: BPI
1108750].
= Heterosporium stromatigenum Bubák & Vleugel, in Bubák, Ann. Mycol. 14:
351. 1916 [type: BPI 802196].
= Cladosporium herbarum f. stellariae Unamuno, Bol. Soc. Esp. Hist. Nat. 34:
146. 1934 [holotype: MA 06330].
= Heterosporium atopomerum Kirschst., Ann. Mycol. 37: 122. 1939 [holotype:
B].
= Cladosporium sarraceniae Dearn. & House, Circ. New York State Mus. 24:
58. 1940, nom. inval.
≡ Cladosporium punctatum Dearn. & House, in herb., non C. punctatum
(Sacc.) Sacc., 1882.
= Cladosporium moldavicum Fosteris, Bull. Sect. Sci. Acad. Roumaine 26(7):
494. 1944? and in Săvulescu, Herb. Mycol. Roman., Fasc. 27, No. 1341. 1944,
nom. inval. [syntypes: BPI 427266, MA-Fungi 8381, etc.].
= Heterosporium equiseti H.C. Greene, Amer. Midl. Naturalist 44(3): 642. 1950
[holotype: WIS].
= Cladosporium herbarum f. amaranthi Petrak, Fl. Bohem. Morav. Exs. Pilze,
II. Ser., 1. Abt. No. 1216.
= Cladosporium hederae, in herb. (HBG).
= Heterosporium caricis Grove, in herb. (K).
= Heterosporium pseudoplatani Grove, in herb. (K).
= Cladosporium herbarum f. Bambusae arundinaceae Thüm., in herb. (M).
Lit.: Saccardo (1886: 350, 1972: 327, 1304), Lindau (1907: 800,
1910: 795), Ferraris (1912: 331), Gonzáles-Fragoso (1927: 194),
de Vries (1952: 71), Hughes (1958: 750), Ellis (1971: 313), Domsch
et al. (1980: 204), Sivanesan (1984: 225), Ellis & Ellis (1985: 290,
468; 1988: 168), Prasil & de Hoog (1988), Wang & Zabel (1990:
202), McKemy & Morgan-Jones (1991c), Dugan & Roberts (1994),
David (1997: 59), Ho et al. (1999: 129), de Hoog et al. (2000:
587), Samson et al. (2000: 110, 2001), Zhang et al. (2003: 103–
108), Heuchert et al. (2005: 47–48), Schubert (2005b: 156–159),
Schubert et al. (2007b: 122–125).
Ill.: Ferraris (1912: 327, ig. 101), de Vries (1952: 73, ig. 15),
Yamamoto (1959: 2, igs 1–4), Ellis (1971: 314, ig. 217 A), Domsch
et al. (1980: 206, ig. 83), Arx (1987: 57, ig. 27), Prasil & de Hoog
(1988: 51, ig. 3), McKemy & Morgan-Jones (1991c: 311, pl. 1;
313, ig. 1), Dugan & Roberts (1994: 516, igs 4–7), David (1997:
62, ig. 17 F, G, I), Ho et al. (1999: 130, igs 21–22), de Hoog et
al. (2000: 587–588, igs), Samson et al. (2000: 110, ig. 49; 111,
pl. 47), Zhang et al. (2003: 107–108, ig. 66–67), Schubert et al.
(2007b: 124–126, igs 16–18).
Exs.: Baglietto et al., Erb. Critt. Ital. 1175, 1396; Bartholomew, Fungi
Columb. 2215, 3613, 4407, 4712, 4713; Beck & Zahlbruckner,
Krypt. Exs. 220; Briosi & Cavara, Fung. Paras. Piante Colt. Util.
Ess. 79; Cooke, Fungi Brit. Exs. 188; Desmazières., Pl. Crypt. N.
France 304; Ellis & Everhart, Fungi Columb. 163 p.p., 383, 1082;
Ellis & Everhart, Fungi Columb. 383, 789; Ellis & Everhart, N. Amer.
Fungi 650, 652, 659, 2968, 2969; Eriksson, Fungi Paras. Scand.
Exs. 500; Fl. Hung. Exs. 206; Fuckel, Fungi Rhen. Exs. 113, 114,
1909, 2561; Jaap, Fungi Sel. Exs. 175; Jack, Leiner & Stizenberger,
Krypt. Badens 327; Jaczewski, Komarov & Tranzschel, Fungi
Ross. Exs. 249, 296; Kabát & Bubák, Fungi Imp. Exs. 241, 242,
344, 443; Kari, Fungi Exs. Fenn. 100; Karsten, Fungi Fenn. Exs.
189; Keissler, Krypt. Exs. 2837 p.p.; Kellerman & Swingle, Ohio
Fungi 124; Liro, Mycoth. Fenn. 825–832; Marcucci, Unio Itin. Crypt.
42; Mougeot & Nestler, Stirp. Crypt. Vog.-Rhen. 299; Oudemans,
Fungi Neerl. Exs. 298; Petrak, Fl. Bohem. Morav. Exs. Pilze 513 a,
1102, 1216; Petrak, Fungi Eichleriani 48; Rabenhorst, Fungi Eur.
Exs. 77, 272, 577, 578, 579, 1271, 1283, 1284, 3895; Rabenhorst,
Klotzschii Herb. Viv. Mycol. 333, 765; Roumeguère, Fungi Sel. Gall.
Exs. 59, 258, 439, 950, 951, 2157, 2158, 2364, 3293, 3391, 4190;
155
BenSch et al.
Saccardo, Mycoth. Ital. 391, 590, 1187, 1189, 1385–1387, 1583;
Saccardo, Mycoth. Ven. 588, 1070, 1071; Săvulescu, Herb. Mycol.
Roman. 1341; Seymour & Earle, Econ. Fungi 502; Sydow, Mycoth.
Germ. 349, 946, 1781, 2044; Sydow, Mycoth. March. 1790, 1795,
4294, 4576, 4577; Thümen, Fungi Austr. Exs. 1293; Thümen, Herb.
Mycol. Oecon. 107, 161, 204, 469, 613; Thümen, Mycoth. Univ.
286, 586, 784, 981, 1767, 1963; Triebel, Microfungi Exs. 72; Vill,
Fungi Bav. 897.
Ascomata pseudothecial, black, globose, erumpent to supericial,
up to 200 μm diam, with 1(–3) short, periphysate ostiolar necks;
wall consisting of 3–6 layers of medium red-brown textura
angularis. Asci fasciculate, bitunicate, subsessile, obovoid to
broadly ellipsoid, straight to slightly curved, 8-spored, 65–85 × 13–
17 μm. Pseudoparaphyses absent in host material, but remnants
observed when studied in culture, hyaline, septate, subcylindrical,
anastomosing, 3–4 μm wide. Ascospores tri- to multiseriate,
overlapping, hyaline, with irregular luminar inclusions, thickwalled, straight to slightly curved, fusoid-ellipsoidal with obtuse
ends, widest near middle of apical cell, medianly 1-septate, not to
slightly constricted at the septum, tapering towards both ends, but
more prominently towards the lower end, (17–)20–23(–25) × (6–)
7(–8) μm; becoming brown and verruculose in asci. Ascospores
germinating after 24 h on MEA from both ends, with spore body
becoming prominently constricted at the septum, but not distorting,
up to 7 μm wide, hyaline to pale brown and appearing somewhat
verruculose, enclosed in a mucoid sheath, with germ tubes being
irregular, somewhat nodular.
In vivo: Mycelium usually immersed, rarely supericial. Stromata
lacking or only with small, loose stromatic hyphal aggregations.
Colonies effuse, caespitose to punctiform-pustulate, velvety,
olivaceous-green,
olivaceous-grey
to
olivaceous-brown.
Conidiophores solitary, loosely aggregated, caespitose or in small
to moderately large, loose to moderately dense fascicles, usually
arising from immersed hyphae or swollen hyphal cells, occasionally
from stromatic hyphal aggregations, erect, straight to somewhat
geniculate-sinuous, usually distinctly nodulose, 30–250 × 3–6(–7)
µm, usually septate, pale to dark olivaceous, olivaceous-brown,
chestnut-brown or brown, wall thin to somewhat thickened, up to 1(–
1.5) µm wide, smooth to somewhat rough-walled, swellings up to 9
µm wide. Conidiogenous cells integrated, terminal and intercalary,
10–30 µm long, nodulose to nodose, swellings usually multilateral,
occasionally more unilateral due to a stronger geniculation,
usually with several conidiogenous loci conined to swellings,
somewhat protuberant, distinctly coronate, (1–)1.5–2(–2.5) µm
diam. Conidia catenate, in simple and branched chains, ellipsoidovoid, subcylindrical, small terminal and intercalary conidia 5–15 ×
(3–)4–6(–7) µm, 0–1-septate, secondary ramoconidia 10–25(–30)
× (4–)5–7(–9) µm, (0–)1–3-septate, not constricted at the septa,
pale to mid olivaceous, olivaceous-brown or brown, thin-walled (≤
1 µm), verruculose to verrucose, rarely a few intermixed conidia
almost smooth, ends obtuse, rounded to slightly attenuated, hila
somewhat protuberant, distinctly coronate, (1–)1.5–2(–2.5) µm
diam.
In vitro: Mycelium supericial, loosely branched, (0.5–)1–5 μm
wide, septate, sometimes constricted at septa, hyaline, subhyaline
to pale brown, smooth or almost so to verruculose or irregularly
rough-walled, sometimes appearing irregular in outline due to
small swellings and constrictions, walls unthickened to somewhat
thickened, cell lumen appearing to be granular. Conidiophores both
156
macro- and micronematous, arising laterally from plagiotropous
hyphae or terminally from ascending hyphae. Macronematous
conidiophores erect, straight to lexuous, somewhat geniculatesinuous, nodulose to nodose with unilateral or multilateral swellings,
with a single to numerous swellings in short succession giving the
stalk a knotty/gnarled appearance, unbranched or occasionally
branched, up to three times, sometimes with a lateral branch-like
proliferation below or at the apex, 10–320 × 3.5–5 μm, swellings
5–8(–9) μm wide, pluriseptate, septa sometimes constricted when
formed after a node, pale to medium brown, older ones almost dark
brown, paler towards the apex, smooth or minutely verruculose,
walls thickened, sometimes even two-layered. Conidiogenous
cells integrated, terminal or intercalary, nodulose to nodose,
with a single or up to ive swellings per cell, 10–24 μm long,
proliferation sympodial, with several conidiogenous loci conined to
swellings, mostly situated on small lateral shoulders, more or less
protuberant, broadly truncate to slightly convex, 1.5–2.5 μm diam,
thickened and somewhat darkened-refractive. Micronematous
conidiophores hardly distinguishable from hyphae, sometimes only
as short lateral outgrowth with a single apical scar, short, conical to
almost iliform or narrowly cylindrical, non-nodulose, not geniculate,
unbranched, 5–120 × 1.5–3(–4) μm, pluriseptate, not constricted
at septa, cells usually very short, 5–15 μm long, subhyaline to
pale brown, almost smooth to minutely verruculose or irregularly
rough-walled, sometimes forming clavate conidia, up to 33 μm
long, 0–2-septate. Conidiogenous cells integrated, terminal or
conidiophores reduced to conidiogenous cells, narrowly cylindrical
or iliform, with a single or two loci. Conidia catenate, in unbranched
or loosely branched chains with branching mostly occurring in the
lower part of the chain, straight to slightly curved, small terminal
conidia without distal hilum obovoid, 4–10 × 3–5(–6) μm [av. ± SD,
7.8 (± 1.9) × 4.7 (± 0.9) μm], aseptate, intercalary conidia with a
single or sometimes up to three distal hila limoniform, ellipsoid to
subcylindrical, 6–16 × 4–6 μm [av. ± SD, 12.4 (± 1.6) × 5.3 (± 0.6)
μm], 0–1-septate, secondary ramoconidia with up to four distal hila,
ellipsoid to cylindrical-oblong, 12–25(–35) × (3–)5–7(–9) μm [av. ±
SD, 18.8 (± 4.5) × 6.2 (± 0.9) μm], 0–1(–2)-septate, rarely with up to
three septa, sometimes distinctly constricted at the septum, septum
median or somewhat in the upper or lower half, pale greyish brown
or brown to medium brown or greyish brown, minutely verruculose
to verrucose, walls slightly to distinctly thickened, guttulate to
somewhat granular, usually only slightly attenuated towards apex
and base, apex obtuse or slightly truncate, towards the base
sometimes distinctly attenuated with hila situated on short stalklike prolongations, hila slightly to distinctly protuberant, truncate to
slightly convex, (0.8–)1–2.5(–3) μm wide, 0.5–1 μm high, somewhat
thickened and darkened-refractive; microcyclic conidiogenesis
occurring, conidia forming micro- and macronematous secondary
conidiophores.
Culture characteristics: Colonies on PDA reaching 19–37 mm diam
after 14 d at 25 ºC, grey-olivaceous to olivaceous-grey, whitish
to smoke-grey or pale olivaceous-grey due to abundant aerial
mycelium, velvety, reverse olivaceous-grey or iron-grey, margin
almost colourless, regular, entire edge, glabrous to feathery,
aerial mycelium abundant mainly in the colony centre, dense,
felty, woolly, sometimes becoming somewhat reddish brown,
fawn coloured, growth regular, lat to low convex with an elevated
colony centre, sometimes forming few large prominent exudates,
sporulation profuse. Colonies on MEA reaching 17–37 mm diam
after 14 d at 25 ºC, smoke-grey to pale olivaceous-grey towards
margin, olivaceous-grey to iron-grey reverse, velvety, margin white,
the genuS Cladosporium
entire edge to slightly undulate, aerial mycelium abundant, dense,
luffy to felty, growth low convex or raised, radially furrowed, folded
and wrinkled in the colony centre, without prominent exudates but
sporulating. Colonies on OA reaching 12–28 mm diam after 14
d at 25 ºC, olivaceous-grey to iron-grey, due to abundant aerial
mycelium pale olivaceous-grey, olivaceous-grey reverse, margin
narrow, more or less undulate, white, aerial mycelium white, loose
to dense, high, luffy to felty, covering large parts of the colony,
growth lat to low convex, without prominent exudates, sporulating.
Substrate and distribution: On fading and decaying plant material,
on living leaves (phylloplane fungus), as secondary invader, as an
endophyte, isolated from air, soil, foodstuffs, paints, textiles and
numerous other materials; cosmopolitan.
Additional specimens examined: (selection; the complete, large collections
deposited at B, HAL, HBG and M have been revised): Sine loco, on Agaricus sp.
(Agaricales), Jun. 1924, Prof. Lehmwanger (M-0057570, as C. fuligineum); on
leaves of Chionanthus sp. (Oleaceae) (L 910.255-872 = L-0115833, holotype of
Dematium epiphyllum var. (β) chionanthi); on Hypholoma fasciculare, 21 Jun. 1905,
O. Jaap (HBG, as C. fuligineum); “ad Gandam”, on leaves of Rhamnus alaterna
(Rhamnaceae), winter 1861, E. Coemans, Rabenhorst, Fungi Eur. Exs. 579 (M0057455, lectotype of C. caespiticium, selected here; HAL, HBG, M-0057454:
isolectotypes). Sine loco et dato, on leaves of Hedera sp. (Araliaceae) (HBG,
original material of C. hederae). Austria, Wiener Wald, on bark of Carpinus
betulus (Betulaceae), May 1936, K. Keissler (PRM 657474). Canada, Ontario,
London, Victoria park, on fallen leaves of Fraxinus sp. (Oleaceae), Oct./Nov. 1896
(DAOM, NYS, syntypes of C. acutum). Czech Republic, Mähren, in den Eisgruber
Weingärten, on leaves of Sorghum bicolor (= Andropogon sorghum, Sorghum
vulgare) (Poaceae), 15 Sep. 1907, Hugo Zimmermann (BPI 426832, as C. graminum
var. sorghi); near Reichenberg, on leaves and culms of Carex (Cyperaceae) (PRM,
type of Cladosporium caricicola Corda). France, Lothringen, Forbach, Behrener
Wald, on Russula sp. (Russulaceae), 15 Sep. 1918, A. Ludwig (B 700006386, as
C. fuligineum). Germany, Bavaria, near Wilzhofen, on Agaricus sp., 28 Oct. 1925,
Schoman (M-0057565, as C. fuligineum); Brandenburg, Kreis Nieder-Barnim,
forest between Sophienstädt and Ruhlsdorf, on decaying gills of Boletus bovinus
(= Suillus bovinus) (Boletaceae), 7 Nov. 1917, P. Sydow, Sydow, Mycoth. Germ.
1781 (M-0057567, as C. fuligineum, mixed collection with C. episclerotiale); Kreis
Prignitz, Triglitz, mould lawn on decaying gills of Pholiota adiposa (Strophariaceae)
on pastures, 27 Dec. 1897, O. Jaap (HBG, as C. fuligineum); Triglitz, on Fraxinus
excelsior, 7 Apr. 1898, O. Jaap (HBG, as C. elegans Penz.); 12 Aug. 1897, O. Jaap
(HBG, as C. elegans Penz.); Hessen, Wiesbaden-Dotzheim, on dead leaves of
Vinca minor (Apocynaceae), 23 Apr. 1963, R. Steppan, No. 1074 (B 700006766, as
“Ramularia vincae Sacc.”, revised by U. Braun as C. vincae Moesz); Kreis Siegen,
near Gernsdorf, on Amanita muscaria (Amanitaceae), 22 Sep. 1935, A. Ludwig
(B 700006385, as C. fuligineum); Kreis Siegen-Wittgenstein, near Wasserburg,
Hainchen, on Boletus subtomentosus, 16 Jul. 1922, A. Ludwig (B 700006382,
neotype of C. fuligineum); Sachsen, Hirschberg, Schmilka near Schandau, on living
leaves of Prunus domestica (Rosaceae), Jul. 1896, G. Wagner (M-0057518, as C.
condylonema); Sachsen-Anhalt, Halle, botanical garden, on the surface of living
leaves of Corylus avellana (Betulaceae), 11 Jun. 2004, B. Heuchert, det. K. Schubert
(HAL); Harz, Schierke, ca. 1.5 km E of town, Königsmoor, on dead leaves attached
to stem, sphagnum bog ex Lycopodium annotinum (Lycopodiaceae), 26 Jul. 2003,
F. Klenke (KR-12198). Hungary, Gebirgspark, on Fraxinus excelsior, Oct. 1886,
J.A. Bäumler, Flora Posoniensis (HBG, as C. elegans Penz.). Italy, on upper and
lower surface of dead leaves of Carex nigra [“fusca”] (Cyperaceae), Tassi no. 862,
(RO, holotype of Davidiella tassiana); Apenninen, Giogo di Scarparia (Mugello), on
still living leaves of Larix europaea (= Larix decidua) (Pinaceae), Sep. 1905, Prof.
V. Perona (PAD, holotype of C. laricis); Cadine, on fallen leaves of Fraxinus sp.,
Nov. 1922, Roui (BPI 426449, as C. desmotrichum); Padova, botanical garden, on
living leaves of Citrus medica (C. ×limonum) (Rutaceae), Aug. 1902, associated
with Epicoccum vulgaris, Saccardo, Mycoth. Ital. 1189 (HBG, neotype of C.
elegans Penz., designated here; Saccardo, Mycoth. Ital. 1189: isoneotypes, e.g.,
PAD); Parma, on living leaves of Prunus domestica, Jun. 1889, Briosi & Cavara,
Fung. Paras. Piante Colt. Utili Ess. 79 (e.g., BPI 426388, HAL, syntypes of C.
condylonema); Riva-Valsesia, on leaves of Salix incana (Salicaceae), 28 Apr. 1891,
Carestia, No. 770 (PAD, holotype of C. fusicladium); Sardinia, Gonnos-Fanadiga,
on leaves of Nerium oleander (Apocynaceae), Dr. Marcucci, Marcucci, Unio Itin.
Crypt. 42 (e.g., HBG, M-0057685, syntypes of C. microporum). Romania, Moldova,
Neamţ District, Broşteni, Neagră Valley, on Festuca ovina (Poaceae), 15 Aug.
1943, S. Fosteris, Săvulescu, Herb. Mycol. Roman. 1341 (BPI 427266; MA-Fungi
8381, syntypes of C. moldavicum). Russia, Kaliningrad area, Polesskij District,
on dead wood of Carpinus betulus (Betulaceae), 10 Oct. 2005, D.A. Shabunin, ex
www.studiesinmycology.org
SPbFRI 238 (HAL); St. Petersburg, Komarov Botanical Garden, near the building
of botanical museum, on fallen leaves of Ginkgo biloba (Ginkgoaceae), 27 Nov.
2002, V. Mel’nik (HAL). Sweden, Prov. Vesterbotten, Umeå, on Alnus incana var.
borealis (Betulaceae), Sep. 1911, J. Vleugel (BPI 426104, holotype of C. alnicola
Bubák & Vleugel). UK, London, Highgate, Millield Lane, on Polyporus squamosus
(Polyporaceae), 14 Oct. 1864, ex herb. Cooke (K 121555, syntype of Heterosporium
epimyces Cooke & Massee). USA, California, on dead leaves of Hedera helix, Dec.
1880, Henkney, No. 1954, mixed infection with a second hyphomycete (K 121546,
holotype of C. brunneum Cooke & Harkn.; BPI 426168: isotype); Colorado, San
Juan Co., above Little Molas Lake, isolated from stems of Delphinium barbeyi
(Ranunculaceae), 12 Sep. 2004, A. Ramaley, CBS H-19868 (teleomorph), single
ascospore isolates, CBS 121622 = CPC 11600, CPC 11601–11604; Mississippi,
Starkville, on leaves of Phalaris canariensis (Poaceae), 25 Mar. 1890, S.M. Tracy,
No. 1323 (NY, holotype of C. velutinum; BPI 427595, 427596, 427597: topotypes);
New Jersey, Newield, on leaves of Euonymus japonicas (Celastraceae), 17 Apr.
1881, J.B. Ellis, no. 3585 (NY, lectotype of C. punctulatum, selected here; BPI
427402, PAD: isolectotypes); New York, Buffalo, on rotten wood (ties), ex herb. G.W.
Clinton (PH); Lyndonville, on dead leaves of Vinca minor, 6 May 1910, C.E. Fairman
[CUP-F2873(24-68), holotype of C. vincae Fairm.]; Saratoga Luke, on Russula sp.,
Apr. 1944, H.D. House (M-0057569, as C. fuligineum); Pennsylvania, Bethlehem,
on leaves of Allium cepa and Allium sp. (Amaryllidaceae), Schweinitz, No. 2602 (PH
1020413, 1020414, syntypes of C. sparsum).
Notes: Cladosporium herbarum is one of the most common and
widespread cosmopolitan hyphomycetes, occurring on all kinds
of organic debris, as secondary invader, indoor fungus, culture
contamination, etc. The connection of the anamorph and Davidiella
tassiana, the sexual stage of this species, was proven by means
of molecular sequence analyses (Schubert et al. 2007b) and
conirmed previously published taxonomic treatments of von Arx
(1950) and Barr (1958). The typiication of C. herbarum proposed
in de Vries (1952) with a specimen from Link’s herbarium at B was,
however, incorrect and had to be refused. Therefore, Prasil & de
Hoog (1988) discussed this typiication and designated one of
Persoon’s original specimens as lectotype of C. herbarum, which
is rather poor, but nevertheless in full accordance with the current
concept of this species and an epitype designated by Schubert et
al. (2007b). The holotype of Davidiella tassiana (RO), the sexual
stage, is morphologically also in good agreement with ascomata
in the epitype.
Type collections of the numerous synonyms of C. herbarum
were previously revised by Hughes (1958), Prasil & de Hoog
(1988) and David (1997). Numerous additional types were reexamined during the course of monographic studies of the genus
Cladosporium carried out by the authors.
78. Cladosporium heterophragmatis S.A. Khan & Kamal,
Mycopathol. Mycol. Appl. 18(4): 246. 1962. Figs 166, 167.
Holotype: Pakistan, Tandoja, Campus A.R.I., on leaves of
Haplophragma adenophyllum (≡ Heterophragma adenophyllum)
(Bignoniaceae), 15 Nov. 1961, Shakil Ahmad Khan (IMI 90787).
Lit.: Schubert & Braun (2004: 298–300), Schubert (2005b: 87–89).
Ill.: Khan & Kamal (1962: 247, ig.), Schubert & Braun (2004: 299,
ig. 1), Schubert (2005b: 88, ig. 35, pl. 15, igs D–H).
In vivo: On living leaves, forming subcircular, oval-oblong to irregular
discolorations on the upper leaf surface, conluent, yellowish brown
or ochraceous. Colonies hypophyllous, caespitose, effuse, dense,
grey-brown to dark brown, blackish, not vein-limited, velvety,
conluent, covering large areas of the leaf surface. Mycelium
external, supericial, hyphae branched, 2.5–6 µm wide, septate,
sometimes slightly constricted at the septa, pale olivaceous to
olivaceous-brown, smooth, rarely rough-walled to verruculose, walls
157
BenSch et al.
Fig. 167. Cladosporium heterophragmatis (IMI 90787). A. Symptoms. B.
Conidiophores arising from supericial hyphae. C. Branched conidiophore and
conidia. D. Small conidiophores arising from plagiotropous hyphae. E. Conidia.
Scale bars = 10 (B–E) µm.
Fig. 166. Cladosporium heterophragmatis (IMI 90787). Conidiophores and conidia
in vivo. Scale bar = 10 µm. K. Bensch del.
thickened, hyphae often aggregated, forming horizontal threads, or
with swollen hyphal cells, subcircular-ellipsoid, up to 13 µm diam.
Conidiophores solitary, arising from swollen hyphal cells or creeping
hyphae, lateral or terminal, erect or sometimes decumbent, straight
to somewhat lexuous, unbranched or branched, 4–113(–190)
× 2–5 µm, often slightly attenuated towards apex, continuous or
with few septa, not constricted at the septa, pale olivaceous to
olivaceous-brown, concolorous with the hyphae, often growing like,
hardly distinguishable from and confusable with supericial hyphae,
smooth, occasionally verruculose. Conidiogenous cells integrated,
terminal, rarely intercalary, cylindrical to oblong, 3–14 µm long,
proliferation sympodial, with a single to several conidiogenous
loci, mostly crowded at the apex, scars protuberant, conspicuous,
sometimes subdenticulate, slightly convex, 1–2 µm diam,
thickened, more or less darkened-refractive. Conidia catenate,
frequently in branched chains, straight to slightly curved, variable
in shape, small conidia (apex without any hila or with a single
apical hilum) subglobose, ellipsoid-ovoid, obovoid, fusiform, 3–14
× 2–4.5 µm, 0–1(–2)-septate, larger conidia with two to several
apical hila (secondary ramoconidia) ellipsoid, fusiform, cylindrical
to somewhat irregular, 5–23(–27) × 3–5(–6) µm, 0–3(–4)-septate,
sometimes slightly constricted at the septa, pale olivaceous, pale
brown to olivaceous-brown, smooth, rarely verruculose, walls
unthickened or only slightly thickened, apex rounded or attenuated,
hila conspicuous, subdenticulate, slightly convex, (0.5–)1–1.5 µm
diam, thickened, darkened-refractive; microcyclic conidiogenesis
occasionally occurring.
Substrate and distribution: On Haplophragma adenophyllum;
Pakistan.
158
Notes: This species is morphologically similar to the mycophilic
species C. phyllophilum, but the latter species is easily
distinguishable by having longer and somewhat wider, usually
multibranched, pluriseptate conidiophores (20–250 × 3–7 µm)
and wider, 0–5(–6)-septate conidia [small conidia 4–13 × 3–6 µm,
large conidia 10–30(–35) × 4–10 µm] (Braun 2001, Heuchert et al.
2005). Cladosporium bignoniae, described on capsules of Bignonia
radicans from North America, is tentatively considered a nomen
dubium (Schubert & Braun 2004), and C. jacarandicola, known from
New Zealand on Jacaranda mimosifolia, differs in having usually
fasciculate conidiophores arising from substomatal stromata.
79. Cladosporium hillianum Bensch, Crous & U. Braun,
Stud. Mycol. 67: 52. 2010. Figs 168, 169.
Holotype: New Zealand, Auckland, St. Johns, Auckland University
campus, artiicial pond, isol. from leaf mold of Typha orientalis
(Typhaceae), 29 May 2008, R. Beever NZ 2008/2765b (CBS
H-20436). Ex-type cultures: CBS 125988 = CPC 15459, CPC
15458.
Ill.: Bensch et al. (2010: 54, igs 41−42).
In vitro: Mycelium internal and supericial; hyphae branched, 1−4.5
μm wide, becoming swollen with age, up to 6 μm wide, single cells
9 μm wide, pluriseptate, narrower hyphae usually not constricted
at septa, but wider ones often slightly to distinctly constricted, due
to swellings, branchings and constrictions often irregular in outline,
sometimes septa in short succession, pale to medium olivaceousbrown, smooth, sometimes slightly rough-walled, walls unthickened
or somewhat thickened, forming subglobose to globose dense
conglomerations (teleomorph initials?), pseudoparenchymatous,
textura angularis, 29−55 μm diam, composed of somewhat swollen
the genuS Cladosporium
Fig. 168. Cladosporium hillianum (CBS 125988). Mycelium, hyphal conglomeration,
conidiophores and conidia in vitro. Scale bar = 10 µm. K. Bensch del.
hyphal cells, 3.5−6 μm wide, medium brown or olivaceous-brown,
slightly thick-walled. Conidiophores solitary, semimacronematous
or micronematous, often hardly distinguishable from supericial
hyphae, arising terminally and laterally from plagiotropous
or ascending hyphae, erect or ascending, straight to slightly
lexuous, cylindrical-oblong, sometimes once geniculate-sinuous
and subnodulose, (12−)25−100 × (3−)3.5−4.5 μm, pluriseptate,
2−8-septa, sometimes slightly constricted at septa, septa often
not very conspicuous, pale olivaceous-brown or pale brown,
smooth, walls slightly thickened, cell structure somewhat unusual,
guttulate. Conidiogenous cells integrated, terminal, occasionally
also intercalary, cylindrical, 8−24 μm long, sometimes geniculatesinuous or subnodulose, with a single locus or often up to four
loci at or towards the apex, situated on small lateral shoulders,
not very conspicuous, 1−1.5(−2) μm diam, slightly thickened,
not darkened but sometimes slightly refractive. Ramoconidia
occasionally formed. Conidia catenate, in long unbranched or
basely branched chains, branching often dichotomously, up to
7(−11) conidia in the unbranched terminal part, small terminal
conidia obovoid to ellipsoid, (5−)6.5−11 × (2−)2.5−4 μm (av. ± SD:
8.0 ± 1.8 × 3.2 ± 0.7), 0−1-septate, septum median or somewhat in
the upper half, intercalary conidia ellipsoid to fusiform or irregular,
7.5−14(−17.5) × 2.5−3.5(−4.5) μm (av. ± SD: 11.0 ± 2.9 × 3.2 ±
0.5), 0−1(−2)-septate, septum median or somewhat in the upper
half, secondary ramoconidia fusiform, ellipsoid to subcylindrical or
irregular, 10−20(−30) × (2.5)3−4.5(−5) μm (av. ± SD: 16.1 ± 4.8 × 3.7
± 0.7), 0−3(−4)-septate, sometimes slightly to distinctly constricted
at septa and becoming sinuous with age, subhyaline to pale brown
or pale to medium olivaceous-brown, smooth, walls unthickened
or slightly thickened, with 1−2(−4) distal hila, subconspicuous to
Fig. 169. Cladosporium hillianum (CBS 125988). A–G. Conidiophores and conidia. H. Hyphal conglomeration. Scale bars = 10 µm.
www.studiesinmycology.org
159
BenSch et al.
conspicuous, (0.5−)0.8−1.5 μm diam, slightly thickened, somewhat
darkened-refractive; microcyclic conidiogenesis often occurring
forming secondary conidiophores and conidia, often germinating.
80. Cladosporium hypophyllum Fuckel, Jahrb.
Nassauischen Vereins Naturk. 23–24: 356. “1869”, 1870.
Figs 170, 171.
Culture characteristics: Colonies on PDA attaining 13−17 mm
diam after 14 d, olivaceous-grey to iron-grey, reverse iron-grey
to olivaceous-black, luffy, margins narrow, colourless to white,
somewhat feathery, slightly undulate, aerial mycelium luffy, loose,
high, pale olivaceous-grey, growth low convex. Colonies on MEA
reaching 24−25 mm diam after 14 d, olivaceous-grey with patches
of white due to aerial mycelium, reverse olivaceous-grey to irongrey, velvety, margin narrow, white, crenate, radially furrowed,
glabrous, aerial mycelium whitish to pale olivaceous-grey, dense,
growth low convex to convex with elevated and wrinkled colony
centre. Colonies on OA attaining 25−30 mm diam after 14 d,
iron-grey with patches of smoke-grey due to aerial mycelium and
sporulation, reverse leaden-grey to olivaceous-grey, margins white,
regular, glabrous, aerial mycelium loose to dense, luffy, smokegrey or whitish, growth lat, sporulating on all media, without
prominent exudates.
Lectotype (designated here): Germany, “auf Grünau bei
Hattenheim am Rheinufer”, on the lower surface of living leaves of
Ulmus minor (= U. campestris) (Ulmaceae), Fuckel, Fungi Rhen.
Exs. 1629 (M-0057614). Isolectotypes: Fuckel, Fungi Rhen. Exs.
1629 (e.g., B 700006555; BPI 427228, 427229, 427234; HAL).
Substrate and distribution: On Typha; New Zealand.
Notes: Cladosporium hillianum, which is unique among
other Cladosporium spp. due to the formation of globoid
pseudoparenchymatic hyphal aggregations on SNA, clustered in
phylogenetic analyses close to C. chalastosporoides but formed
a distinct lineage (Bensch et al. 2010, ig. 1). The latter species is
easily distinguishable from C. hillianum by its distinctly narrower
conidiophores, aseptate, narrower conidia and 0−1(−2)-septate
secondary ramoconidia. Cladosporium delicatulum is another
supericially similar species, which differs, however, in forming
numerous globose to broadly ellipsoid, shorter and narrower small
terminal conidia and some other minor differences. In addition,
pseudoparenchymatous conglomerations of textura angularis as in
C. hillianum are not produced.
= Cladosporium microstictum Sacc. & D. Sacc., in Saccardo, Ann. Mycol. 3:
169. 1905, syn. nov. [lectotype: B 700006625].
Lit.: Saccardo (1886: 360), Lindau (1907: 820), Schubert (2005b:
89–91).
Ill.: Schubert (2005b: 90, ig. 36, pl. 17, igs A–E).
Exs.: Fuckel, Fungi Rhen. Exs. 1629; Saccardo, Mycoth. Ital. 589.
In vivo: On living leaves, without distinct leaf spots, hypophyllous
as greyish olivaceous coating. Colonies hypophyllous, effuse,
loosely to densely caespitose, pale to medium olivaceous-brown or
brown, sometimes even blackish, sometimes covering large areas
of the leaf surface. Mycelium external, supericial; hyphae iliform,
branched, 1–4 µm wide, septate, sometimes constricted at the
septa, subhyaline to pale olivaceous-brown, later distinctly swollen,
up to 7 µm wide, constricted at the septa, darker, pale medium
to medium olivaceous-brown or brown, walls somewhat thicker,
almost smooth or mostly more or less verruculose to irregularly
rough-walled, protoplasm of the cells sometimes aggregated at the
septa so that walls and, above all, septa appear to be thickened (as
in distoseptation), with a somewhat irregular cavity in the centre
of the cells, hyphae aggregated, forming loose to dense hyphal
ropes or plates and stromatic hyphal aggregations composed of
subglobose swollen hyphal cells, brownish to somewhat reddish
brown, walls thickened. Conidiophores solitary or in loose groups,
not fasciculate, arising from external creeping hyphae, lateral or
Fig. 170. Cladosporium hypophyllum (M-0057614). Conidiophores and conidia in vivo. Scale bar = 10 µm. K. Bensch del.
160
the genuS Cladosporium
Fig. 171. Cladosporium hypophyllum (M-0057614). A. Conidiophores, conidia and external mycelium. B. Geniculate conidiophore. C. Old, distinctly swollen conidia showing cell
structure, with paler cavity in the centre. D, E. Conidia. Scale bars = 10 (A–E) µm.
terminal, or from stromatic hyphal aggregations, erect, straight to
lexuous, often mildly to distinctly geniculate-sinuous, sometimes
subnodulose, unbranched or branched, 10–80(–100) × (2–)2.5–
4(–5) µm, septate, pale olivaceous-brown, smooth to somewhat
verruculose-asperulate, walls thickened, sometimes even twolayered, sometimes growing like and confusable with hyphae,
protoplasm of the cells occasionally aggregated at the septa,
appearing to be thickened (as in distoseptation). Conidiogenous
cells integrated, terminal and intercalary, often geniculate to
subnodulose, 8–25 µm long, proliferation sympodial, with
several conidiogenous loci, often situated on small shoulders or
unilateral swellings but not conined to them, subdenticulate,
protuberant, obconically truncate or slightly convex, 0.5–1.5 µm
diam, thickened, somewhat refractive, but mostly not darkened.
Conidia catenate, in unbranched or branched chains, straight,
ovoid, ellipsoid to fusiform, sometimes subcylindrical, 4–17(–19)
× 2–5 µm, 0–1(–3)-septate, subhyaline to pale olivaceous, with
age becoming distinctly swollen, longer and wider, 5–7 µm wide,
usually constricted at the septa, darker, pale medium olivaceousbrown to somewhat reddish brown, with thicker walls, confusable
with swollen hyphal cells of stromatic hyphal aggregations, smooth
or almost so to more or less verruculose or irregularly rough-walled,
protoplasm often distinct, clearly delineated from the inner wall and
sometimes with a conspicuous, small, irregular, paler lumen in the
centre of the cells, giving conidia a somewhat zonate appearance,
ends rounded or slightly attenuated, hila more or less protuberant,
0.5–1.5 µm diam, thickened, refractive but mostly not darkened;
microcyclic conidiogenesis often occurring.
www.studiesinmycology.org
Substrate and distribution: On Ulmus minor (Ulmaceae); Europe
(Germany, Italy).
Additional specimens examined: Italy, Treviso, Vittorio, on leaves of Ulmus
minor (= U. campestris), Oct. 1899, D. Saccardo, Saccardo, Mycoth. Ital. 589
(B 700006625, lectotype of C. microstictum, designated here; isolectotypes: D.
Saccardo, Saccardo, Mycoth. Ital. 589, e.g. HBG).
Notes: De Vries (1952) listed C. hypophyllum as synonym of C.
cladosporioides. A re-examination of type material of C. hypophyllum
revealed that the latter species is quite distinct in having usually
geniculate conidiophores with several conidiogenous loci, shorter
conidia and somewhat narrower, mostly not darkened conidiogenous
loci and hila; the unusual cell structure as described above does not
occur in C. cladosporioides. Cladosporium heliotropii, which has a
similar growth type and cell structure, is easily distinguishable by
having usually non-geniculate, somewhat wider conidiophores with
only few conidiogenous loci and somewhat wider conidia. A single
collection deposited at herbarium M under C. hypophyllum (Germany,
Bavaria, Fürstenfeldbrück, at the bank of the river Amper towards
Schoengasing, on still living leaves of Ligustrum vulgare, 31 Aug.
1883, Allescher, M-57613) proved to be C. herbarum as secondary
invader occurring on leaf spots caused by Septoria ligustri.
Cladosporium microstictum, also described on leaves of Ulmus
minor, was introduced in 1899 in D. Saccardo’s “Mycotheca italica”
without any description. Later, in 1905, Saccardo published a
detailed Latin diagnosis. Type material of this species could be reexamined and proved to be synonymous with C. hypophyllum.
161
BenSch et al.
Fig. 172. Cladosporium inconspicuum (B 700006557). Conidiophores and conidia in
vivo. Scale bar = 10 µm. K. Bensch del.
81. Cladosporium inconspicuum Thüm., Contributiones ad
lorum mycologicam lusitanicam, Ser. 2, No. 193. 1879 and
Hedwigia 19: 133. 1880. Figs 172, 173.
Lectotype (designated here): Portugal, Coimbra, botanical
garden, on living leaves of Styrax oficinalis (Styracaceae), Oct.
1878, G.A. Moller (B 700006557).
Lit.: Saccardo (1886: 359), Oudemans (1923), Schubert (2005b:
91–92).
Ill.: Schubert (2005b: 92, ig. 37, pl. 16, igs G–I).
reddish brown, centre mostly paler, pale greyish brown or whitish
grey, margin narrow, dark brown, sometimes surrounded by a
somewhat reddish brown halo, spots turning fragile with age, on the
lower leaf surface almost inconspicuous. Colonies hypophyllous,
scattered, brownish, only sparingly fruiting. Mycelium internal
and external, supericial; hyphae branched, (1–)3–6 µm wide,
septate, with swellings and constrictions, then up to 9 µm wide,
pale to medium smutty brown, rarely subhyaline, smooth, walls
somewhat thickened, forming hyphal ropes and plates or loose
to somewhat denser stromatic hyphal aggregations composed of
swollen hyphal cells, somewhat darker than hyphae, medium dark
brown, thick-walled. Conidiophores solitary, arising terminally or
laterally from supericially growing, creeping hyphae or stromatic
hyphal aggregations, erect, straight or somewhat lexuous,
narrowly cylindrical, non-geniculate, non-nodulose, unbranched,
19–45 × 3–4(–5) µm, septate, pale brown or yellowish brown,
smooth, walls somewhat thickened. Conidiogenous cells
integrated, usually terminal, 4–10 µm long, proliferation
sympodial, with a single or few conidiogenous loci, subdenticulate,
obconically truncate, 0.5–1.5(–2) µm diam, thickened, refractive
to somewhat darkened. Conidia in unbranched or branched
chains, obovoid, ovoid-ellipsoid to fusiform, 4–14(–20) × 2.5–5
µm, 0–1(–3)-septate, not constricted at the septa, pale brown,
smooth or almost so (verruculose under SEM), walls somewhat
thickened, often slightly attenuated towards the base, hila
protuberant, obconically truncate, 0.5–1.5(–2) µm diam, central
dome usually not higher than the surrounding rim and often not
very conspicuous, thickened, refractive to somewhat darkened;
microcyclic conidiogenesis not observed.
Substrate and distribution: On Styrax oficinalis; Portugal.
Notes: Thümen (1880) described long, sometimes branched and
somewhat wider (5 µm) conidiophores which have not been seen
during the course of the re-examination of the type material of C.
inconspicuum. The type is in poor condition and only sparingly
fruiting. Only few conidia and conidiophores have been observed.
SEM examinations conclusively showed that C. inconspicuum
belongs in Cladosporium s. str. (Fig. 173A), but additional
collections need to be examined to determine the morphological
variation in this taxon. Urtiaga (1986) recorded this species from
Cuba on Begonia nelumbifolia, but this could not be conirmed.
In vivo: Leaf spots epiphyllous, small, subcircular to somewhat
irregular, 1–5 mm wide, medium to dark brown or somewhat
Fig. 173. Cladosporium inconspicuum (B 700006557). A. Tip of a conidiophore showing the coronate scar structure. B, C. Conidiophores. Scale bars = 5 (A), 10 (B–C) µm.
162
the genuS Cladosporium
Fig. 174. Cladosporium inopinum
(GZU). Conidiophores and conidia in
vivo. Scale bar = 10 µm. U. Braun del.
82. Cladosporium inopinum (Petr.) U. Braun, Mycotaxon
55: 224. 1995. Fig. 174.
Basionym: Cercospora inopina Petr., Sydowia 4: 570. 1950.
Lectotype (designated here): Ecuador, Pichincha, near Quito, on
leaves of Gynoxys hallii (Asteraceae), 20 Sep. 1937, H. Sydow,
Poelt & Scheuer, Reliqu. Petrak. 1350 (GZU). Isolectotypes: Poelt
& Scheuer, Reliqu. Petrak. 1350.
Lit.: Chupp (1954: 142), Crous & Braun (2003: 227), Schubert
(2005b: 93).
Ill.: Braun (1995a: 225, ig. 2), Schubert (2005b 93, ig. 38).
Exs.: Poelt & Scheuer, Reliqu. Petrak. 1350.
In vivo: Leaf spots indistinct. Mycelium mainly external, on
trichomes; hyphae branched, 1–5 µm wide, septate, subhyalinegreenish, olivaceous to brown, smooth, rarely faintly rough-walled,
forming small stromata. Stromata composed of brown swollen
cells, 4–9 µm wide, fairly thick-walled. Conidiophores solitary to
fasciculate, arising from small stromata or plagiotropous hyphae,
erect or horizontal, straight to geniculate-sinuous, subcylindrical,
unbranched, occasionally branched, 10–120 × 3–7 µm, continuous
or septate, sometimes with constrictions and swollen segments,
olivaceous-brown, smooth, walls only slightly thickened.
Conidiogenous cells integrated, terminal or conidiophores reduced
to conidiogenous cells, cylindrical, often geniculate, with a single
or few conidiogenous loci, somewhat protuberant, about 1–2 µm
diam, thickened and darkened. Conidia catenate, occasionally in
branched chains, ellipsoid-ovoid, subcylindrical, (8–)10–30(–35) ×
2.5–6 µm, 0–3(–4)-septate, olivaceous to brown, smooth to faintly
rough-walled, hila somewhat protuberant, about 1.5–2 µm diam,
thickened and darkened.
Substrate and distribution: On Gynoxys hallii; Ecuador.
Notes: Based on the cladosporioid structure of the conidiogenous
loci and hila, Cercospora inopina was transferred to Cladosporium
s. str. (Braun 1995a). Cladosporium gynoxidicola, also known
www.studiesinmycology.org
from Ecuador on leaves of Gynoxys sp., has been excluded from
the latter genus and re-allocated to Passalora (Schubert & Braun
2005b).
83. Cladosporium inversicolor Bensch, Crous & U. Braun,
Stud. Mycol. 67: 55. 2010. Figs 175, 176.
Holotype: Netherlands, isol. from a leaf of Triticum aestivum
(Poaceae), deposited Jul. 1980 as C. cladosporioides, isol. by N.J.
Fokkema, ident. by G.A. de Vries (CBS H-20437). Ex-type culture:
CBS 401.80 = ATCC 200941.
Ill.: Bensch et al. (2010: 55−56, igs 43−44).
In vitro: Mycelium immersed and sparingly supericial; hyphae
mainly unbranched, 1.5−3(−4.5) μm wide, septate, not constricted
at septa, without swellings, pale olivaceous to pale olivaceousbrown, smooth to often minutely verruculose, walls unthickened.
Conidiophores macronematous, solitary, arising terminally and
laterally from hyphae, erect, straight to somewhat lexuous,
cladosporioides-like, cylindrical-oblong, somewhat geniculatesinuous towards or at the apex, non-nodulose, unbranched or
once branched, 15−225 × 2.5−4(−5) μm, aseptate or with few
septa, not constricted at septa, subhyaline to very pale olivaceousbrown, smooth, sometimes rough-walled at the base; occasionally
also micronematous, about 1.5 μm wide. Conidiogenous cells
integrated, mainly terminal, cylindrical-oblong, non-nodulose,
sometimes geniculate at or towards the apex due to sympodial
proliferation, 15−66 μm long, with (1−)2−3 loci, conspicuous,
subdenticulate, 1−2 μm diam, somewhat thickened and darkenedrefractive. Ramoconidia occasionally formed, cylindrical-oblong,
17−42 × 3−3.5 μm, aseptate, occasionally with up to three septa,
base (1.8−)2−3 μm wide, unthickened. Conidia numerous, catenate,
in branched chains, often dichotomously branched, sometimes in
more directions, terminal unbranched parts of the chains often
very long, up to eight conidia, sometimes even up to 17 conidia,
small terminal conidia obovoid to ellipsoid, sometimes subglobose,
163
BenSch et al.
conidia ovoid, fusiform to ellipsoid, (5−)7−20 × (2−)2.5−3.5(−4)
μm (av. ± SD: 10.3 ± 3.5 × 2.9 ± 0.4), aseptate, attenuated
towards apex and base, with 1−3(−4) distal hila, secondary
ramoconidia subcylindrical, 10.5−24(−29) × (2.2−)2.8−4(−4.2) μm
(av. ± SD: 16.9 ± 4.0 × 3.3 ± 0.5), 0−1(−2)-septate, but mainly
aseptate, not constricted at septa, pale to olivaceous-brown,
small terminal conidia and intercalary conidia slightly darker than
ramoconidia, secondary ramoconidia and conidiophores, smooth
to loosely minutely verruculose or irregularly rough-walled, rugose,
verruculose-rugose surface ornamentation especially in small
terminal and intercalary conidia, conidia slightly attenuated towards
apex and base, with (1−)2−4(−6) distal hila, walls unthickened or
almost so, hila conspicuous, subdenticulate, 0.5−2 μm diam,
thickened and darkened-refractive; microcyclic conidiogenesis not
observed.
Fig. 175. Cladosporium inversicolor (CBS 401.80). Conidiophores and conidial
chains in vitro with intercalary conidia and small terminal conidia sometimes
verruculose or irregularly rough-walled, rugose. Scale bar = 10 µm. K. Bensch del.
(3−)5−8.5 × 2−3(−3.5) μm (av. ± SD: 5.9 ± 1.6 × 2.6 ± 0.4),
aseptate, apex rounded, attenuated towards the base, intercalary
Culture characteristics: Colonies on PDA attaining 60−75 mm diam
after 14 d, olivaceous-grey, grey-olivaceous towards margins,
leaden-grey to olivaceous-black reverse with grey-olivaceous
margins, loccose, margins regular, white or colourless, feathery,
aerial mycelium sparse to abundant, diffuse to loccose, loose to
dense, growth effuse, without prominent exudates, sporulation
profuse. Colonies on MEA 62−65 mm diam after 14 d, greyolivaceous to olivaceous-grey or olivaceous, reverse iron-grey
to black, velvety, loccose to felty, margins colourless or white,
glabrous to feathery, regular, aerial mycelium whitish to smokegrey, felty-loccose, growth effuse, sometimes radially furrowed
in colony centre, without exudates, sporulation profuse. Colonies
on OA 50−65 mm diam after 14 d, grey-olivaceous to greenish
olivaceous, olivaceous, olivaceous-grey or olivaceous-buff, reverse
pale greenish grey to olivaceous-grey, leaden-grey or iron-grey,
Fig. 176. Cladosporium inversicolor (CBS 401.80). A–G. Conidiophores and conidial chains with intercalary conidia and small terminal conidia somewhat darker than ramoconidia
and secondary ramoconidia. Scale bars = 10 µm.
164
the genuS Cladosporium
velvety to loccose, margins glabrous, olivaceous-grey, narrow,
aerial mycelium smoke-grey to pale olivaceous-grey, felty, growth
lat, without exudates, sporulation profuse.
Substrates and distribution: On plant material, isol. from air and
food, also mycophilic; Europe (Denmark, Germany, Netherlands),
North America (USA), South America (Colombia).
Additional specimens examined: Sine loco, isol. from Triticum aestivum (Poaceae)
by F.T. Bennett, 1929, deposited as C. cladosporioides, CBS 131.29 = ATCC
200942 = ATCC 11275 = IMI 049623 = LCP 52.404. Colombia, isol. from Cortaderia
sp. (Poaceae), CBS 484.80. Denmark, Usseroed, isol. from school dust, 2000, B.
Andersen, BA 1735 = CPC 14368. Germany, Bayern, München, park of castle
Nymphenburg, isol. from Puccinia bromina ssp. symphyti-bromarum var. paucispora,
Jul. 2006, K. Schubert, CPC 13150. Netherlands, isol. from a leaf of Tilia sp.
(Tiliaceae), deposited Jan. 1965 as C. cladosporioides, isol. by A.A. Verhorst, ident.
by G.A. de Vries, CBS 143.65; Baarn, de Vuursche, isol. from seeds of Alnus sp.
(Betulaceae), 14 Mar. 1982, G.S. de Vries, No. 4110, CBS H-1604, CBS 464.82
= ATCC 200945, deposited as C. laxicapitulatum; Millingerwaard, isol. from a fruit
of Sambucus nigra (Adoxaceae), 29 Aug. 2007, P.W. Crous, CPC 14241; Zwolle,
isol. from outside air, 7 Jan. 2007, M. Meijer, CPC 14190, 14191. USA, Seattle,
University of Washington campus, 47.6263, 122.3331, isol. from chasmothecia of
Phyllactinia guttata (Erysiphales) on leaves of Corylus avellana (Betulaceae), 16
Sep. 2004, D. Glawe, CPC 11818.
Notes: Cladosporium inversicolor belongs to the C. cladosporioides
complex. The name of this species is derived from the unusual
pigmentation of conidia with intercalary conidia being usually darker
than ramoconidia, secondary ramoconidia and conidiophores,
which is unique and distinctive amoung Cladosporium species of
this complex and in general. Phylogenetically C. inversicolor is
close to C. delicatulum (Bensch et al. 2010, ig. 1, part a), which
is, however, morphologically well distinguished by having often
dichotomously branched, very long conidial chains and various
other characters of the conidia.
84. Cladosporium iranicum Bensch, Crous & U. Braun,
Stud. Mycol. 67: 56. 2010. Figs 177, 178.
Holotype: Iran, isol. from a leaf of Citrus ×aurantium (= C.
×sinensis) (Rutaceae), on scale insect, 2004, W. Gams (CBS
H-20438). Ex-type culture: CBS 126346 = CPC 11554.
Ill.: Bensch et al. (2010: 56−57, igs 45−46).
In vitro: Mycelium mainly immersed; hyphae sparingly branched,
1−5 μm wide, septate, sometimes with swellings and constrictions,
therefore irregular in outline, almost hyaline to pale or medium
olivaceous-brown, smooth to minutely verruculose, walls
unthickened to slightly thickened, forming ropes. Conidiophores
macro- and micronematous, solitary, arising terminally and laterally
from plagiotropous or ascending hyphae, erect or ascending,
straight to slightly lexuous, iliform to cylindrical-oblong, neither
nodulose nor geniculate, unbranched or once branched,
branches mostly only as short lateral prolongations just below a
septum, 40−180(−325) × (2−)2.5−4(−5) μm, pluriseptate, mostly
2−5-septate, not constricted at septa, few septa darkened (viz.
those septa at the base of potential ramoconidia), subhyaline
to pale, sometimes medium olivaceous-brown, smooth or
almost so at the apex and inely asperulate towards the base,
walls unthickened to slightly thickened. Conidiogenous cells
integrated, mainly terminal, cylindrical-oblong, neither geniculate
nor nodulose, 6−44 μm long, smooth, with up to three apical loci,
sometimes one or two loci at a lower level, denticulate, protuberant,
www.studiesinmycology.org
Fig. 177. Cladosporium iranicum (CBS 126346). Macro- and micronematous
conidiophores, ramoconidia and conidial chains in vitro with intercalary conidia
sometimes subrostrate. Scale bar = 10 µm. K. Bensch del.
1−2 μm diam, thickened and darkened-refractive. Ramoconidia
sometimes formed, cylindrical-oblong, 31−42 × 3.5−5 μm, often
1-septate, base broadly truncate, 2.2−3 μm wide, not thickened,
somewhat refractive. Conidia catenate, in long unbranched and
branched chains, chains often dichotomously branched, up to 10
conidia in the unbranched terminal part, straight to slightly curved,
small terminal conidia and intercalary conidia in long unbranched
chains, obovoid, limoniform to narrowly fusiform or ellipsoid,
4−10 × (1−)1.5−3 μm (av. ± SD: 6.2 ± 1.7 × 2.3 ± 0.6), aseptate,
often subrostrate to rostrate, secondary ramoconidia ellipsoid to
subcylindrical or cylindrical-oblong, 10−33 × (2−)3−4 μm (av. ±
SD: 21.0 ± 6.8 × 3.4 ± 0.5), 0−1(−2)-septate, not constricted at
septa, septum median, sometimes in the lower third, often not very
conspicuous, almost hyaline to pale olivaceous-brown, smooth
or almost so, walls unthickened or slightly thickened, slightly to
distinctly attenuated towards apex and base, cell structure 1−2
guttulate, with up to three distal hila, hila protuberant, denticulate,
0.5−2 μm diam, thickened and darkened-refractive; conidia often
germinating especially terminal ones, germ tubes up to 30 μm long;
no microcyclic conidiogenesis.
Culture characteristics: Colonies on PDA attaining 53−60 mm
diam after 1 mo, olivaceous-grey to grey-olivaceous towards
the margins, reverse iron-grey to leaden-grey, powdery to luffy,
margin regular, white, broad, glabrous, aerial mycelium abundantly
formed, luffy, especially in the colony centre, no exudates formed,
sporulation profuse. Colonies on MEA reaching 47 mm diam after 1
mo, smoke-grey or whitish due to aerial mycelium, pale olivaceousgrey towards margins, reverse olivaceous-grey to buff, luffy-felty,
margin regular, white, glabrous, radially furrowed, aerial mycelium
abundant, luffy, covering the whole surface, no exudates,
sporulation profuse.
165
BenSch et al.
Fig. 178. Cladosporium iranicum (CBS 126346). A–D, F–G. Macronematous conidiophores and conidial chains. E. Conidia and conidiophore showing the often guttulate cell
structure. H–I. Subrostrate intercalary conidia. Scale bar = 10 µm.
Substrate and distribution: On Citrus; Iran.
Notes: Cladosporium iranicum belongs to the C. cladosporioides
complex and is morphologically very close to the latter species
s. str., but somewhat distinct by its 1(−2)-septate secondary
ramoconidia, often ± beaked intercalary conidia and slow-growing
cultures. Phylogenetically C. iranicum is, however, quite unrelated
to C. cladosporioides (Bensch et al. 2010, ig. 1, part a vs. c). There
are some other Cladosporium species described from Citrus spp.,
which are, however, quite distinct.
85. Cladosporium iridis (Fautrey & Roum.) G.A. de Vries,
Contr. Knowl. Genus Cladosporium: 49. 1952. Figs 179, 180.
Basionym: Scolecotrichum iridis Fautrey & Roum., Rev. Mycol.
(Toulouse) 13: 82. 1891.
≡ Heterosporium iridis (Fautrey & Roum.) J.E. Jacques, Contr. Inst. Bot.
Univ. Montréal 39: 18. 1941.
Lectotype: France, Cote d’Or, Jardin de Noidan, on leaves of Iris
germanica (Iridaceae), Jul. 1880, F. Fautrey, Roumeguère, Fungi
Sel. Gall. Exs. 5689 (PC, designated by David 1997). Isolectotypes:
Roumeguère, Fungi Sel. Gall. Exs. 5689, e.g. K.
166
Epitype: Netherlands, Boterenbrood, isolated from Iris sp., Aug.
1940 (CBS H-19859). Ex-epitype culture: CBS 138.40.
= Heterosporium gracile Sacc., Syll. fung. 4: 480. 1886, as “(Wallr. ?) Sacc.”
[lectotype: PAD].
= Heterosporium montenegrinum Bubák, Sitzungsber. Königl. Böhm. Ges.
Wiss. Prag, Math.-Nat. Classe, 1903(12): 21. 1903 [lectotype: BPI 802189].
= Scolecotrichum cladosporioideum Maire, Ann. Mycol. 4: 329. 1906, as “sp.
prov. nov.” [holotype: MPU; isotypes: PC, K].
= Didymellina macrospora Kleb., Ber. Deutsch. Bot. Ges. 42: 60. “1924” 1925.
≡ Mycosphaerella macrospora (Kleb.) Jørst., Meld. Statens Plantepatol. Inst.
1: 20. 1945.
≡ Davidiella macrospora (Kleb.) Crous & U. Braun, Mycol. Progr. 2(1): 10.
2003.
= ? Heterosporium pruneti Nicolas & Aggéry, Rev. Pathol. Vég. Entomol. Agric.
France 15: 66. 1928.
= Heterosporium iridis-pumilae Săvul. & Sandu, Hedwigia 75: 222. 1935
[syntypes: K, IMI 10047, M-0057611].
Lit.: Saccardo (1886: 480, 1892: 600), Ellis (1971: 312), Ellis &
Waller (1974), Sivanesan (1984: 222, as Cladosporium state of
Mycosphaerella macrospora), McKemy & Morgan-Jones (1990),
David (1997: 43), Shin et al. (1999), Zhang et al. (2003: 111),
Schubert et al. (2007b: 125–129).
Ill.: Ellis (1971: 311, ig. 215 B), Sivanesan (1984: 224, ig. 120), McKemy
& Morgan-Jones (1990: 427, ig. 1; 429, pl. 1; 431, ig. 2; 433, ig. 3; 437,
pl. 2), David (1997: 45, ig. 11), Shin et al. (1999: ig. 1), Zhang et al.
(2003: 111, ig. 71), Schubert et al. (2007b: 128, igs 20–21).
the genuS Cladosporium
the cuticle, erect, straight to substraight or lexuous, often slightly
to distinctly geniculate, subnodulose to torulose, unbranched, later
occasionally branched, (12–)25–120(–250) × (6–)9–15(–17) µm,
swellings up to 17 µm wide, continuous to septate, pale to medium
olivaceous-brown or brown, somewhat paler at the apices, smooth or
occasionally minutely roughened with age, walls almost unthickened,
becoming more thick-walled with age, base often swollen, up to 27
µm wide. Conidiogenous cells integrated, terminal or intercalary, 10–
94 µm long, subnodulose, geniculate, proliferation sympodial, with a
single or few conidiogenous loci situated on small lateral shoulders or
swellings, loci protuberant, coronate with a distinct periclinal rim, 2−4
µm wide, thickened, somewhat darkened-refractive. Conidia solitary
or in short unbranched chains, straight to slightly curved, oblongcylindrical, becoming soleiform with age, (13–)30–60(–75) × (11–)
15–23(–28) µm, (0–)2–4(–7)-septate, often slightly constricted at
the septa, septa becoming sinuous with age, pale to medium brown
or olivaceous-brown, distinctly and densely spinulose echinulate, at
irst thin-walled (young conidia) becoming thick-walled, up to 2–3(–
5) µm thick including ornamentation, rounded at both ends, rarely
somewhat pointed, with a distinctly bulbous base, hila often not very
prominent, often only visible as thickened and somewhat darkened
plate just below the outer echinulate wall layer, in younger conidia
protuberant, 2.5–4 µm wide, thickened; microcyclic conidiogenesis
not observed.
Fig. 179. Cladosporium iridis (CBS 138.40). Conidiophores and conidia in vitro.
Scale bar = 10 µm. K. Bensch del.
Exs.: Anonymous, Fl. Olten. Exs. 151; Baglietto, Cesati & Notaris,
Erb. Critt. Ital. Ser. II, 1299; Briosi & Cavara, Fungi Paras. Piante
Colt. Utili Ess. 115; Ellis & Everhart, N. Amer. Fungi. Ser. II, 2189;
Kabát & Bubák, Fungi Imperf. Exs. 295, 595, 796; Krieger, Fungi
Saxon. Exs. 2448; Newodowski, Griby Ross. Herb. 194; Petrak,
Fl. Bohem. Morav. Exs. Pilze 521, 2210, 2479; Săvulescu, Herb.
Mycol. Roman. 187, 648; Scheuer & Poelt, Mycoth. Graec. 54;
Sydow, Mycoth. Germ. 1396; Sydow, Mycoth. March. 1683, 4398;
Triebel, Microf. Exs. 535; Vestergren, Micromyc. Rar. Sel. Praec.
Scand. 241; Vill, Fungi Bav. 790; Weese, Eumyc. Sel. Exs. 611;
Zahlbruckner, Krypt. Exs. 1836b.
In vivo: Leaf spots amphigenous, necrotic, oval-ellipsoid to oblong,
sometimes subcircular, 1−8 mm long, 0.5−2 mm wide, scattered,
with a pale yellowish, greyish to pale brown centre, surrounded by a
narrow to broad reddish brown to brown or even dark margin and a
somewhat wider olivaceous-green halo, later often conluent. Colonies
amphigenous, in small tufts, punctiform to effuse, caespitose, dark
olivaceous-brown to brown, villose, fruiting on the pale centre of the
spots, often distinctly visible (even without using a stereomicroscope).
Mycelium internal, subcuticular to intraepidermal; hyphae sparingly
branched, 3–12 µm wide, septate, not constricted at the septa,
subhyaline to pale olivaceous, smooth, walls unthickened or almost
so. Stromata small to extended, 40−150 × 30−80 µm or conluent and
larger, several layers deep, substomatal to intraepidermal, medium to
almost dark brown, composed of swollen hyphal cells, subglobose
to angular, 10−16(−22) µm wide, pale olivaceous, smooth, walls
only slightly thickened. Conidiophores solitary or usually fasciculate,
in small to moderately large loose fascicles, caespitose, arising
from stromata, emerging through stomata or erumpent through
www.studiesinmycology.org
In vitro: Mycelium branched, 2–8 μm wide, septate, not constricted
at the septa, hyaline to pale brown, smooth, walls slightly
thickened, sometimes guttulate. Conidiophores very long, usually
terminally arising from ascending hyphae, erect to subdecumbent,
slightly to distinctly lexuous, geniculate-sinuous, usually several
times, subnodulose due to geniculate, sympodial proliferation
forming swollen lateral shoulders, unbranched, rarely branched,
up to 720 μm long, 6–11 μm wide, swellings 8–11(–14) μm wide,
pluriseptate, often very regularly septate, not constricted at
the septa, pale to medium olivaceous-brown, somewhat paler
towards the apex, smooth to minutely verruculose, walls only
slightly thickened. Conidiogenous cells integrated, terminal as
well as intercalary, cylindrical-oblong, 15–55 μm long, proliferation
percurrent to sympodial, usually with a single geniculation forming
laterally swollen shoulders often below a septum, conidiogenous
loci conined to swellings, usually one locus per swelling, rarely
two, protuberant, (2–)2.5–4 μm diam, somewhat thickened
and darkened-refractive. Conidia solitary, sometimes in short,
unbranched chains, straight to curved, young conidia pyriform to
subcylindrical, connection between conidiophore and conidium
being rather broad, subhyaline to pale olivaceous-brown, walls
slightly thickened, then enlarging and becoming more thick-walled,
cylindrical-oblong, soleiform with age, both ends rounded, usually
with a slightly to distinctly bulbous base, visible from a very early
stage, but broadest part often towards the apex not at the base, (18–)
30–75(–87) × (7–)10–16(–18) μm [av. ± SD, 53.3 (± 17.8) × 12.6 (±
2.2) μm], (0–)2–6(–7)-septate, usually not constricted at the septa,
rarely slightly constricted, septa often becoming sinuous with age,
pale to medium olivaceous-brown, sometimes darker, verrucose to
echinulate, walls thickened, especially in older conidia, up to 1 μm
thick, hila protuberant, often stalk-like or conically prolonged, up
to 2 μm long, (2–)2.5–3.5(–4) μm diam, with age becoming more
sessile, sometimes just visible as a thickened plate just below the
outer wall layer, especially in distal scars of branched conidia,
periclinal rim often distinctly visible, hila somewhat thickened and
darkened-refractive; microcyclic conidiogenesis not observed.
167
BenSch et al.
Fig. 180. Cladosporium iridis (CBS 138.40). A–C. Conidiophores with conidia. D. Conidium. Scale bar = 10 µm.
Culture characteristics: Colonies on PDA reaching 19–23 mm
iam after 14 d at 25 ºC, pale greenish olivaceous, smoke-grey
to olivaceous-grey due to abundant aerial mycelium, greenish
olivaceous to olivaceous reverse, margin broad, regular, entire
edge to slightly undulate, feathery, aerial mycelium abundantly
formed, felty, luffy, covering large parts of the colony, mainly in
the central parts, high, growth low convex with a somewhat raised
colony centre. Colonies on MEA reaching 9–23 mm diam after 14 d
at 25 ºC, pale olivaceous-grey to olivaceous-grey, olivaceous-grey
reverse, felty, margin slightly undulate, white, somewhat raised,
aerial mycelium abundant, loose, diffuse, high, growth low convex,
radially furrowed, slightly folded. Colonies on OA reaching 10–19
mm diam after 14 d at 25 ºC, olivaceous, margin broad, undulate,
white, aerial mycelium white, very high, loose, diffuse, hairy, growth
lat, due to the mycelium low convex, without prominent exudates
and sporulating on all media. Cultures on SNA without sporulation.
Substrates and distribution: Leaf spot and blotch of Belamcanda,
Gladiolus, Iris, Juno and Pardanthopsis spp. (Iridaceae),
Hemerocallis (Xanthorrhoeaceae), Africa (Algeria, Morocco, South
Africa, Zambia, Zimbabwe), Asia (Armenia, Azerbaijan, China,
Georgia, India, Iran, Israel, Japan, Kazakhstan, Kyrgyzstan, Korea,
Russia, Turkey, Turkmenistan, Uzbekistan), Australasia (Australia,
New Zealand), Caucasus, Europe (Austria, Belgium, Belorussia,
Cyprus, Czech Republic, Denmark, Estonia, France, Germany,
Great Britain, Greece, Italy, Latvia, Lithuania, Malta, Moldavia,
Montenegro, Netherlands, Norway, Poland, Romania, Russia,
Serbia, Spain, Sweden, Ukraine), North America (Canada, USA),
Central and South America (Argentina, Chile, Jamaica, Panama,
Uruguay) – Belamcanda chinensis (= Gemmingia chinensis)
(China, Jilin; USA, CA, FL, IA, KS, NY, OK, VA, VT, WI), Gladiolus
×gandavensis (Czech Republic), Gladiolus spp. (Denmark, Iran,
Romania), Hemerocallis fulva (USA, NJ), Iris alberti (Kazakhstan),
I. anguifuga (China, Zhejiang), I. aphylla (= I. furcata) (Armenia,
Romania), I. bismarkiana (Romania), I. crocea (Germany), I.
elegantissima (Armenia), I. ensata (China, Gansu), I. ×lorentina
(Romania), I. foetida (= I. gueldenstaedtiana) (Czech Republic,
Serbia, Sweden), I. foetidissima (France, Germany, Italy, New
Zealand), I. germanica (Armenia, Australia, Austria; Canada, BC,
NS, Ont.; Czech Republic, Denmark, Estonia, France, Germany,
168
Hungary, Israel, Italy, Korea, New Zealand, Poland, Romania,
Serbia, South Africa, Spain; USA, WA), I. glaucescens (= I. scariosa
auct.) (Kazakhstan), I. halophila (Armenia, Germany, Kazakhstan,
Romania), I. humilis (= I. lavissima, I. mandshurica (Kazakhstan,
Romania), I. hungarica (Romania), I. iberica (Romania), I. japonica
(China, Xinjiang; Romania), I. kamaonensis (Germany, Romania),
I. latifolia (Germany), I. monnieri (Romania), I. musulmanica
(Armenia), I. nepalensis (Germany), I. pallida (Latvia, Romania),
I. paradoxa (Armenia), I. plicata (= I. swertii Hort.) (Denmark), I.
prilipkoana (= I. demetrii) (Armenia), I. pseudocyperus (Greece,
Romania), I. pumila (Armenia, Romania), I. sibirica (Armenia), I.
sogdiana (Armenia, Kazakhstan), I. songarica (Romania), I. spuria
(Denmark, Romania), I. spuria subsp. halophila (Germany), I.
tectorum (China, Anhui, Jiangsu, Liaoning, Shandong), I. tingitana
(New Zealand), I. tuberosa (Romania), I. variegata (Romania), I.
versicolor (Armenia, Romania, USA, MA, OK), I. xiphium (Canada,
BC; New Zealand), Iris spp. (Armenia; Australia, Tasmania; Austria;
Canada, BC, Man., NB, NS, Ont., Que.; China, Jilin; Czech
Republic, Denmark, Germany, Greece, Iran, the Netherlands, New
Zealand, Poland, Central and South America, South Africa; USA,
AL, FL, HI, NC, WY; Sweden, Ukraine), Juno bucharica (≡ Iris
bucharica) (Romania), J. caucasica (≡ I. caucasia) (Romania),
Pardanthopsis dichotoma (≡ Iris dichotoma) (China, Jilin).
Additional specimens examined: Sine loco et sine dato, isolated from Iris sp.,
CBS 107.20. Austria, Steiermark, Graz, Bezirk Liebenau, Murfeld, GF 8958/4,
47°01’N/15°27’E, 340 m alt., in einem Hausgarten, on Iris sp., 18 Jun., 9 Aug., 20
Aug. 1996, P. Zwetko, Scheuer & Poelt, Mycoth. Graec. 54 (M-0057610); Wien,
botanical garden, on I. germanica, Jul. 1915, Kupka (M). Czech Republic, Böhmen,
Turnau, on I. gueldenstaedtiana, 16 Jul. 1909, J.E. Kabát, Kabát & Bubák, Fungi
Imperf. Exs. 796 (M); Brünn in Mähren, on Gladiolus ×gandavensis, Sep. 1925,
Dr. J. Hruby, Petrak, Fl. Bohem. Morav. Exs. Pilze 2210 (M); on Iris sp., Aug. 1929,
Dr. J. Hruby, Petrak, Fl. Bohem. Morav. Exs. Pilze, 2479 (M); Eisgrub I Mähren,
Fürst Lichtenstein Hofgarten, on I. germanica, 28 Aug. 1912, H. Zimmermann,
Petrak, Fl. Bohem. Morav. Exs. Pilze 521 (M); Sternberg, Park der Irrenanstalt, on
I. germanica, Dec. 1925, J. Piskoř, Flora moravica. France, Iles de Chausey, on I.
foetidissima, 11 Jul. 1929, W.J. Lütjeharms, Fungi gallici (M). Germany, Bayern,
Freising, on I. germanica, 1897, Prof. J.E. Weiss (M); Murnau, on I. germanica,
Sep. 1875, Allescher (M); Mittelfranken, Kreis Hersbruck, on I. germanica, 27 Aug.
1946, K. Starcs, No. 2495 (M); Oberfranken, Bamberg, on I. germanica, Aug. 1902,
A. Vill, Vill, Fungi Bav. 790 (M); Berlin, botanical garden, on Iris sp., Aug. 1890, P.
Magnus, (M); Späth’sche Baumschule, on I. germanica, 1895, P. Sydow, No. 79
(M); Sachsen, Rathen, in a garden, on Iris sp., Jul. 1916, W. Krieger, Krieger, Fungi
Saxon. Exs. 2448 (M); Sachsen-Anhalt, Halle, botanical garden, on Iris crocea,
the genuS Cladosporium
11 Jun. 2004, K. Schubert (HAL); on I. foetidissima, 22 Jun. 2004, K. Schubert
(HAL); on I. kamaonensis, 11 Jun. 2004, K. Schubert (HAL); on I. latifolia, 22 Jun.
2004, K. Schubert (HAL); on I. nepalensis, 11 Jun. 2004, K. Schubert (HAL); on I.
spuria ssp. halophila, 18 Sep. 2003, K. Schubert (HAL); 25 Sep. 2003, K. Schubert
(HAL); Thüringen, Weimar, in a garden, on I. germanica, Oct., J. Bornmüller,
Zahlbruckner, Krypt. Exs. 1836b (M). Greece, Athen, on I. pseudacorus, Apr., F. von
Höhnel, Zahlbruckner, Krypt. Exs. 1836b (M; same exsiccatae number as above but
different dates). Israel, Jerusalem-Catamon, on I. germanica, 2 Mar. 1938, Dr. T.
Rayss, Flora Cryptog. Palaestinae. Italy, Pavia, botanical garden, on I. germanica,
1889, Briosi & Cavara, Fung. Paras. Piante Colt. Utili Ess. 115 (M); Pozzuolo nel
Lucchese, on I. foetidissima, Apr. 1882, G. Arcangeli, Baglietto, Cesati & Notaris,
Erb. Critt. Ital., Ser. II, 1299 (B). Latvia, Prov. Vidzeme, Riga, on I. pallida, 6 Jul.
1932, K. Starcs, No. 342 (M). New Zealand, Otago, Dunedin, on Lilium sp. (?
Iris sp.), Oct. 1948, J.M. Dingley (PDD 6292). Poland, “Tamsel”, Schlossgarten,
on I. germanica, 16 Jul. 1913, P. Vogel, Sydow, Mycoth. Germ. 1396; Schlesien,
Freudenthal, am Sudetinum des neuen Parkes, on I. germanica, Sep. 1933, Dr.
J. Weese, Weese, Eumyc. Se. Exs. 611 (M). Romania, Muntenia, distr. Ilfov,
Bucureşti, on I. germanica, 27 Jul. 1927, T. Săvulescu & C. Sandu, Săvulescu, Herb.
Mycol. Roman. 187 (M); Burureşti, Grādina Botanica, on I. lorentina, 12 Jul. 1958,
Eliade (M); Mănăstirea, on I. germanica, 1 Aug. 1932, T. Săvulescu & C. Sandu,
Săvulescu, Herb. Mycol. Roman. (M); distr. Oltenia, Gura-Văii, on I. pumila, 13 Jul.
1961, Eliade (M); Oltenia, distr. Craiova, Horto Bot. Craiovense, on I. germanica, alt.
ca. 95 m, 5 Oct. 1963, J. Comes, I. Ene, M. Costescu & I. Terbea, Anonymous, Fl.
Olten. Exs. 151 (M). Serbia, Topčider near Belgrad, on I. gueldenstaedtiana, 14 Jul.
1909, N. Ranojevič, Kabát & Bubák, Fungi Imperf. Exs. 595 (M). Sweden, Uppsala,
botanical garden, on I. gueldenstaedtiana, 18 Sep. 1895, A.G. Eliasson (M); on Iris
sp., 15 Sep. 1896, T. Vestergren, Vestergren, Micromyc. Rar. Se. Praec. Scand. 241
(M). Ukraine, province Kiev, distr. Tscherkasy, Smiela, on Iris sp., 6 Dec. 1915, G.
Newodowski, Newodowski, Griby Ross. Herb. 194 (M). USA, New Jersey, Newield,
on Hemerocallis fulva, Jul. 1888, Ellis & Everhart, N. Amer. Fungi. Ser. II, 2189 (M);
Wyoming, Laramie, on Iris sp., Oct. 1940, W.I. Solheim (M).
Notes: Cladosporium iridis is one of the Heterosporium-like species
with very large conidia formed singly or only in very short chains.
Records of this species on Narcissus sp. (Amaryllidaceae) from
Armenia, Allium canadense (Amaryllidaceae), Chlorogalum sp.
(Asparagaceae), Pandanus sp. (Pandanaceae) and Freesia
hybrid (Iridaceae) from from the USA (Farr et al. 1989) are very
doubtful and possibly based on confusions with C. macrocarpum
or other species. Schubert et al. (2007b) examined collections
on Hemerocallis fulva from the USA as well as Gladiolus
×gandavensis from the Czech Republic and conirmed their correct
determinations. Another collection on the latter host from Germany,
deposited at K (Kew), proved to be misidentiied and belonging to
Drechslera (David 1997).
86. Cladosporium jacarandicola K. Schub., U. Braun &
C.F. Hill, Sydowia 56(2): 300. 2004. Figs 181, 182.
Holotype: New Zealand, Mt. Albert, Ruarangi Road, on living
leaves of Jacaranda mimosifolia (Bignoniaceae), 6 Aug. 2002, C.F.
Hill 693 (HAL 1812 F).
Lit.: Schubert (2005b: 94–95).
Ill.: Schubert & Braun (2004: 300, ig. 2), Schubert (2005b: 94, ig.
39, pl. 17, igs F–I).
In vivo: On living leaves, causing small, subcircular, brownish spots.
Colonies punctiform, scattered, brownish. Mycelium internal; hyphae
3–4(–5) µm wide, septate, pale olivaceous. Stromata absent to welldeveloped, substomatal, composed of subglobose, more or less
thick-walled cells, 5–7 µm wide, olivaceous to olivaceous-brown.
Conidiophores solitary or in loose to dense fascicles, arising from
stromata, emerging through stomata, erect, straight to somewhat
lexuous, somewhat geniculate-sinuous, unbranched, occasionally
branched, 10–58 × 3.5–5 µm, continuous to sparsely septate,
www.studiesinmycology.org
Fig. 181. Cladosporium jacarandicola (HAL 1812 F). Conidiophores and conidia in
vivo. Scale bar = 10 µm. K. Bensch del.
olivaceous, olivaceous-brown, apex often paler, subhyaline, smooth,
walls slightly thickened, somewhat swollen at the base. Conidiogenous
cells integrated, terminal, rarely intercalary, cylindrical-oblong, 10–30
µm long, proliferation sympodial, with several conidiogenous loci,
often crowded at the apex, loci protuberant, 1–1.5(–2) µm diam,
thickened, darkened-refractive. Conidia in unbranched or branched
chains, subglobose, obovoid, fusiform, ellipsoid, 4–18 × 3–5(–6.5)
µm, 0–3-septate, pale olivaceous, smooth to verruculose, walls
not to slightly thickened, apex rounded, somewhat attenuated or
truncate, base truncate to slightly convex, hila (0.5–)1–1.5(–2) µm
diam, thickened, darkened-refractive; microcyclic conidiogenesis
occasionally occurring.
Substrate and distribution: On Jacaranda mimosifolia; New
Zealand.
Notes: There are some morphologically similar species with
consistently short conidiophores, which are, however, quite distinct
from C. jacarandicola in forming wider conidiophores [(3–)4–10(–13)
wide in C. agoseridis; 3–8 µm in C. lupiniphilum], larger, above all
wider conidia with different surface ornamentations [(6.5–)12–26(–
31) × 4–8 µm, faintly to conspicuously verruculose-echinulate in C.
praecox; (10–)12–40(–50) × (5–)6–13(–15) µm, loosely to densely
verruculose or verrucose in C. agoseridis; 6–28 × 4–8 µm, smooth
or almost so in C. lupiniphilum] and wider loci, respectively [1.5–2.5
µm diam in C. orchidearum; (1.5–)2–3.5(–4.5) µm diam. in C.
agoseridis] (Braun & Rogerson 1995, Braun 1998, 2000, Ellis 1976).
In addition to the species of Cladosporium, this collection (no. 693)
also contained rich fructiication of Cercospora apii Fresen. s. lat.
87. Cladosporium lacroixii Desm., Pl. Crypt. N. France, Ed.
3, Fasc. 15–16, No. 755. 1860. Fig. 183.
Lectotype (designated by David 1997): France, Vienne, on
Narcissus jonquilla (Amaryllidaceae), L.-S.V. Lacroix, Desmazières,
Pl. Crypt. N. France 755 (PC). Isolectotypes: Desmazières, Pl.
Crypt. N. France 755, e.g., BR, K, IMI 118411.
169
BenSch et al.
Fig. 182. Cladosporium jacarandicola (HAL 1812 F). A, C. Fascicles of conidiophores emerging through stomata. B. Unbranched and branched conidiophores. D. Conidia.
Scale bars = 10 (A–D) µm.
Lit.: Saccardo (1886: 370), Ellis (1976: 333), David (1997: 50).
Ill.: Ellis (1976: 335, ig. 253), David (1997: 51, ig. 13, 53, ig. 14 G–I).
In vivo: Colonies on necrotic leaves, effuse, olivaceous to blackish
brown, loose to dense, velvety. Mycelium immersed. Stromata
lacking, but solitary swollen hyphal cells or small to moderately
large aggregations of such cells formed, cells up to 15 µm diam.,
brown. Conidiophores solitary to caespitose, arising from swollen
hyphal cells, erect, usually moderately to strongly geniculatesinuous, torulose, usually unbranched, 50–100 × 5–13 µm,
pluriseptate throughout, pale to medium brown, tips sometimes
paler, thin-walled, ≤ 1 µm, smooth or almost so. Conidiogenous
cells integrated, terminal and intercalary, proliferation distinctly
sympodial, 10–35 µm long, conidiogenous loci distinctly coronate,
1–5 per cell, 1.5–3.5 µm wide and about 1 µm high, somewhat
darkened-refractive. Conidia usually solitary, ellipsoid-ovoid,
obovoid, short cylindrical, (15–)20–50(–60) × 9–15(–17) µm,
(0–)1–3(–4)-septate, occasionally somewhat constricted at the
septa, but usually non-constricted, septa often becoming sinuous
with age, very pale to medium brown, wall thin, < 1 µm, densely
to occasionally more loosely verrucose, ends rounded, hila
conspicuously coronate, 1.5–3.5 µm wide, up to 1 µm high.
Substrate and distribution: On Narcissus jonquilla; France.
Fig. 183. Cladosporium lacroixii (IMI 118411). Conidiophores and conidia in vivo.
Scale bar = 10 µm. U. Braun del.
170
Notes: This species is morphologically close to Cladosporium
macrocarpum, but differs by much larger conidia, almost
the genuS Cladosporium
Fig. 184. Cladosporium langeronii (CBS 189.54) (from Zalar et al. 2007). A–D. Colony surface grown on PDA (A), OA (B), MEA (C) and MEA plus 5 % NaCl (D) of strains
incubated for 14 d at 25 ºC in darkness. E–F. Habit of conidiophores. G–I. Ramoconidia and conidia. E–I. All from 7-d-old SNA slide cultures. A–D, from CBS 189.54 (ex-type
strain); E, from CBS 109868; F–I, from EXF-999. Scale bars = 10 (G–I), 30 (F), 100 (E) µm.
consistently formed singly, with septa becoming sinuous with
age. The ecology of this species, which is only known from the
type collection, is unclear. The type material consists of necrotic
leaves. If this fungus is biotrophic or saprobic, remains unclear
and requires new collections. In the type material, several much
narrower conidiophores, up to 100 × 3–5 µm, with much smaller,
Cladosporium herbarum-like conidia have been observed. If they
belong to C. lacroixii, representing a second type of a dimorphic
fruiting is also unclear and could only be proven in culture.
www.studiesinmycology.org
88. Cladosporium langeronii (Fonseca, Leão & Nogueira)
Vuillemin, Champ. Paras. Myc. Homme: 78. 1931. Fig. 184.
Basionym: Hormodendrum langeronii Fonseca, Leão & Nogueira,
Sciencia Med. 5: 563. 1927.
≡ Cladosporium langeronii (Fonseca, Leão & Nogueira) Cif., Manuale di
Micologia Medica, Ed. 2: 488. 1960, comb. superl.
171
BenSch et al.
Neotype: Brazil, isolated from man ulcero-nodular mycosis of hand
and arm, 1927, coll. & isol. by da Fonseca (CBS H-19737). Ex-type
culture: CBS 189.54.
Lit.: Zalar et al. (2007: 173–174).
Ill.: Zalar et al. (2007: 174, ig. 9).
In vitro: Mycelium partly immersed, partly supericial; hyphae
branched, 1–3 µm wide, septate, without swellings and constrictions,
subhyaline to pale brown, smooth or almost so, sometimes
enveloped in polysaccharide-like material, sometimes forming few
swollen hyphal cells, up to 7 µm diam, arranged like a starting
stroma giving rise to several conidiophores appearing loosely
fasciculate. Conidiophores macro- and micronematous, arising
terminally and laterally from submerged and supericial hyphae,
erect or ascending, straight to slightly lexuous. Macronematous
conidiophores cylindrical-oblong, sometimes geniculatesinuous, non-nodulose, (20–)50–210(–290) × 2.5–4.5(–6) µm,
unbranched or often branched, once or several times, branches
not only as short peg-like prolongations but longer, distinct, one
branching often below the apex, pluriseptate, cell length 9–22
µm, not constricted at septa, medium to dark brown, somewhat
paler at the apex, smooth to verruculose or irregularly roughwalled, walls slightly thickened, about 0.5 µm wide, protoplasm
granular. Conidiogenous cells integrated, terminal, sometimes also
intercalary, cylindrical, 9–25 µm long, slightly attenuated at the
apex, sometimes seceding and forming ramoconidia, usually with a
single apical scar, protuberant, 0.8–1.5(–2) µm diam, thickened and
darkened-refractive. Micronematous conidiophores iliform, mostly
unbranched, rarely branched, 6–120 µm long or longer, 1–2 µm
wide, pale brown, septate, smooth or almost so, walls unthickened.
Conidiogenous cells integrated, terminal or sometimes discrete,
with a single apical scar, protuberant, 0.5–1 µm diam, thickened
and darkened-refractive. Ramoconidia cylindrical, 0–1-septate,
(10–)11–22(–42) × (3–)3.5–4.5(–5) µm, base broadly truncate,
2–3.5 µm wide, slightly thickened and somewhat darkened.
Conidia catenate, formed in dichotomously branched chains, with
up to six conidia in the unbranched parts, straight, small terminal
conidia subglobose or ovoid, (3–)4–5.5(–8) × (2–)3–4(–5) µm
(av. ± SD: 5.1 ± 1.6 × 3.1 ± 0.5 μm), aseptate, rarely 1-septate,
hila 0.5–0.8 µm diam, apex rounded, intercalary conidia broadly
ovoid to ellipsoid, 5–11 × 3–4 µm (av. ± SD: 7.5 ± 1.7 × 3.3 ±
0.3 μm), 0(–1)-septate, not constricted, attenuated towards apex
and base, with a single apical hilum, 0.5–1 µm diam, secondary
ramoconidia ellipsoid to cylindrical, (5.5–)9–20(–35.5) × (2.5–)3–
4.5(–5.5) µm (av. 10.7 ± 4.7 × 3.6 ± 0.8 µm), 0–1(–2)-septate,
not constricted at septa, pale to medium or dark brown, irregularly
verruculose to sometimes loosely verrucose, walls slightly or more
distinctly thickened, with 1–2(–3) distal hila, hila protuberant, peglike, denticulate, 0.8–1.5(–2) µm diam, thickened and darkenedrefractive; microcyclic conidiogenesis occasionally occurring.
Conidia formed by micronematous conidiophores paler, narrower,
usually only in unbranched chains, iliform, ellipsoid to obclavate,
3–12 × 1.5–2.5 µm, 0(–1)-septate.
Culture characteristics: Colonies on PDA, OA and MEA with
restricted growth, attaining 2.5–4.5, 1.5–7.0 and 1.0–5.5 mm diam.,
respectively. Colonies lat or heaped (up to 3 mm), dark green,
with black reverse and slightly undulate margin with immersed
mycelium. Sporulating on PDA and OA, sterile on MEA. On MEA
+ 5 % NaCl growth is faster, colonies attaining 8.5–12 mm diam,
sporulating and growing deeply into the agar. Maximum tolerated
172
salt concentration: All strains develop colonies at 17 % NaCl
after 14 d. Cardinal temperatures: No growth at 4 °C, optimum /
maximum at 25 °C (1–5.5 mm diam.), no growth at 30 °C (from
Zalar et al. 2007).
Substrate and distribution: Polar ice and biomats, conifer wood and
window frame in Europe, humans; strains originating from nasal
mucus (Buzina et al. 2003) have 100 % sequence homology with
studied strains, as well as a clone from mycorrhizal roots (Menkis
et al. 2005).
Additional specimens examined: Antarctics, isol. from biomat in a lake (dH
11736). Arctics, isol. from ice (dH 13833 = EXF-1933). Belgium, Lichtervoorde,
isol. from a moist aluminium school window frame (CBS 101880). Brazil, isol.
from orig. face lesions (dH 12459 = EXF-999). Germany, Göttingen, isol. from
wood of Picea abies (CBS 601.84). Italy, Parma, isol. from mortar of Muro
Farnesiano, coll. by C. Urzi, Dpt. Sci. Microbiol. Gen. Mol., Univ. of Messina, Italy,
No. MC 783 (CBS 109868).
Notes: Cladosporium langeronii is a saprobic species belonging to
the C. sphaerospermum complex. This species, which is probably
much more widespread than indicated by the present collections,
is morphologically and genetically allied to the latter species (s.
str.), but clearly distinct (see Zalar et al. 2007). De Vries (1952)
synonymised the isolate identiied as Hormodendron langeronii
with C. sphaerospermum. Other authors confused strains of this
species with C. cladosporioides (Buzina et al. 2003, Menkis et al.
2005), although it has slightly longer conidia. According to Zalar
et al. (2007) it most likely represents a complex of at least two
species with strains from the Arctic and the Antarctic probably being
distinguished from C. langeronii on species level.
Cladosporium langeronii failed to grow at 30 °C and has been
isolated from various saprobic substrates. Therefore, clinical
strains of this species could have been secondary colonisers being
able to dwell on surfaces poor in nutrients or more likely, could be
air-borne contaminations of lesions (Summerbell et al. 2005, Zalar
et al. 2007).
89. Cladosporium leguminicola U. Braun & K. Schub.,
Schlechtendalia 16: 65. 2007. Fig. 185.
Holotype: Spain, Churriana (Málaga), C. Blívar et E. Rijoa, on pods
of Phaseolus vulgaris (Fabaceae), 18 Jun. 1917, Gonz. Fragoso
2906 (MA 06450-52), as “C. pisi”.
Ill.: Braun & Schubert (2007: 66, ig. 3).
In vivo: On necrotic pods, lesions lacking. Colonies dark brown
to blackish, effuse to dense, forming subcircular to irregular
speckles. Mycelium immersed. Stromata small to well-developed,
10–60 µm diam., later enlarged or conluent, up to 150 µm diam.,
yellowish brown, pale to medium brown or medium to dark
olivaceous-brown, cells circular to somewhat angular-irregular
in outline, 3–7 µm diam. Conidiophores in small to large, loose
to dense fascicles, arising from stromata, erect, straight to
somewhat curved or slightly sinuous, but usually non-geniculate,
unbranched, 10–70 × 3–7 µm, 0–3-septate, wall thin to slightly
thickened, ≤ 1 µm, pale olivaceous to medium olivaceousbrown, smooth or almost so to verruculose. Conidiogenous cells
integrated, terminal or conidiophores reduced to conidiogenous
cells, 10–30 µm long, coronate conidiogenous loci 1.5–2.5 µm
wide and 1 µm high. Conidia solitary or in unbranched chains,
the genuS Cladosporium
Fig. 185. Cladosporium leguminicola (MA 06450-52). Conidiophores, conidiogenous
cells and conidia in vivo. Scale bar = 10 µm. U. Braun del.
ellipsoid-ovoid, subcylindrical, ramoconidia not seen, 5–26
× 3–5.5 µm, 0–3-septate, subhyaline to pale olivaceous or
olivaceous-brown, thin-walled, verruculose, ends obtuse, rounded
to somewhat attenuated, hila distinct, slightly protuberant, 1.5–2
µm diam; microcyclic conidiogenesis lacking.
Fig. 186. Cladosporium licheniphilum (LE). Conidiophores and conidia in vivo. Scale
bar = 10 µm. B. Heuchert del.
Substrate and distribution: On Phaseolus vulgaris; Spain.
Notes: The type collection of this species was deposited at MA as
Cladosporium pisi Cugini & Macch. The latter species was described
to have small, subhyaline conidia, 4.5–5.5 × 4–4.5 (terminal) and
7–9 × 2.5–4.5 µm (lateral), respectively (Saccardo 1892: 601).
These measurements rather agree with those of C. cladosporioides,
which is common on pea. Gonzáles-Fragoso (1927) recorded the
collection on Phaseolus vulgaris as C. pisi, but simply copied
the original description of the latter species, which is not in full
agreement with the fungus on bean. Cladosporium cladosporioides
is quite distinct from C. leguminicola by lacking stromata, usually
much longer, iliform, non-fasciculate conidiophores with terminal,
non-geniculate conidiogenous cells and, above all, usually
smooth-walled, 0–1-septate conidia. The true C. pisicola, often
confused with the common C. cladosporioides, is a leaf-spotting
fungus, characterised by having integrated, often intercalary,
distinctly geniculate conidiogenous cells and 0–3-septate conidia.
It is distinct from C. leguminicola by its geniculate conidiophores
with intercalary conidiogenous cells, smooth conidia and lacking
stromata.
90. Cladosporium licheniphilum Heuchert & U. Braun,
Herzogia 19: 12. 2006. Figs 186, 187.
Holotype: Russia, Altai, Zmeinogorsk Region, Belaya River near
Mt. Stanovaya, 51°00’N, 82°44’N, alt. 600 m, Taiga forest, on
apothecia of Pertusaria alpina, 12 Jun. 1999, E. A. Davydov (LE).
Epitype (designated in Bensch et al. 2010): Germany, Bavaria,
Unterfranken, Kreis Aschaffenburg, parking area at the motorway
Würzburg-Frankfurt, south-east of Waldaschaff, isol. from the lichen
Phaeophyscia orbicularis and Physcia sp. on stems and bark of
www.studiesinmycology.org
Acer platanoides (Aceraceae), 31 May 2006, W. von Brackel, No.
3808 (CBS H-20439). Ex-type culture: CBS 125990 = CPC 13224.
Lit.: von Brackel (2007: 8, 2008: 64, 2009), Bensch et al. (2010:
58).
Ill.: Heuchert & Braun (2006: 13, ig. 1; 18, ig. 5 A–F), Bensch et
al. (2010: 59, ig. 47).
In vivo: Colonies on the thallus and on apothecia of the host lichens,
caespitose, reddish brown to dark brown or blackish, somewhat
shiny. Mycelium immersed; hyphae branched, sinuous, 5–8 µm
wide, brown, septate, with constrictions, thick-walled, smooth,
hyphal cells around the conidiophores swollen, subglobose to
polygonal, about 12 µm diam. or 7–17 × 4–10 µm, thick-walled, dark
brown, smooth. Stroma lacking. Conidiophores solitary or in small,
loose tufts, arising from internal swollen hyphal cells, erect, straight
to slightly curved, subcylindrical to slightly geniculate-sinuous,
unbranched to 1–3 times branched, ramiication usually terminal,
divergent, branchlets short to moderately long, 10–55 × 4–6.5 µm
(occurrence and percentage of branches variable, almost lacking
to abundant), conidiophores (65–)90–190 × 5–8 µm, somewhat
wider at the base and gradually narrowed towards the apex,
5–14-septate, usually not constricted at the septa, dark brown,
paler towards the apex, thick-walled (up to 2 µm), but wall of the
terminal conidiogenous cells thinner, smooth, often with a single or
few distant enteroblastic-percurrent proliferations. Conidiogenous
cells integrated, terminal, occasionally intercalary, (7–)9–20 µm
long, subcylindrical-conic to slightly geniculate-sinous, proliferation
sympodial, with a single or usually numerous, up to 12, coronate
conidiogenous loci, 1–2 µm diam. Ramoconidia lacking or very
173
BenSch et al.
Fig. 187. Cladosporium licheniphilum in vivo (LE) and in vitro (CBS 125990). A–G. Macronematous conidiophores and conidial chains in vitro. H. Conidiophores in vivo.
I. Apically branched tips of conidiophores. J–K. Conidia. Scale bars = 10 (A–G, I–K), 20 (H) µm.
rare. Conidia catenate, usually in branched acropetal chains,
subglobose, limoniform to ellipsoid-subcylindrical, 0–2(–3)-septate,
ramoconidia lacking, only with secondary ramoconidia, aseptate
conidia 3.5–8 × 3–5 µm, secondary ramoconidia and septate
conidia 7–20 × 4–7 µm, usually not constricted at the septa, pale
brown or yellowish brown, wall thin to slightly thickened, smooth
or almost so, ends more or less rounded or slightly attenuated,
with a single basal and 1–4 distal hila, coronate, 0.5–2 µm diam;
occasionally with microcyclic conidiogenesis.
In vitro: Mycelium immersed and supericial, dimorphic, sparingly
branched, 2−5 μm wide, septate, not constricted at septa, hyaline
to subhyaline or olivaceous-brown, sterile hyphae smooth,
fertile hyphae giving rise to conidiophores irregularly roughwalled, Zasmidium(Stenella)-like, often irregular in outline,
walls unthickened to somewhat thickened. Conidiophores
macronematous, sometimes also micronematous, arising laterally
or terminally from plagiotropous or ascending hyphae, solitary,
sometimes in loose groups of two or three, erect, straight to slightly
lexuous, cylindrical-oblong, unbranched or once, sometimes
twice branched, lateral branches as short outgrowth just below
a septum or relatively long, in an angle of 45−90°, 28−90(−145)
× 3−4 μm, pluriseptate, not constricted at septa, pale olivaceous
to olivaceous-brown, paler towards apices, smooth to somewhat
irregularly rough-walled, similar as hyphae, walls thickened, up to
1 μm wide; micronematous conidiophores shorter, narrower and
paler, 14−65 × 2−3 μm, septate. Conidiogenous cells integrated,
terminal and intercalary, cylindrical-oblong, 5−35 μm long, with
1−5 protuberant, denticle-like loci, often aggregated or sitting
on small lateral prolongations, broadly truncate, 1.2−2 μm diam,
174
thickened and distinctly darkened, secondary ramoconidia sitting
like a whirl at the tip of conidiophores. Ramoconidia occasionally
formed, with a broadly truncate base, not darkened but slightly
refractive. Conidia numerous, catenate, in branched chains, often
dichotomously branched, especially in the terminal part, small
terminal conidia ovoid to obovoid, sometimes subglobose or
globose, 3−5 × 2−3 μm (av. ± SD: 4.3 ± 0.8 × 2.6 ± 0.4), aseptate,
rounded at the apex, attenuated towards the base, smooth, thinwalled, intercalary conidia ovoid-ellipsoid, 5−8(−12) × 3−4 μm
(av. ± SD: 7.6 ± 1.7 × 3.6 ± 0.4), aseptate, distinctly attenuated
towards the base, with 1−3 distal hila, sometimes situated on short
lateral prolongations at the distal end, secondary ramoconidia
ellipsoid to fusiform or subcylindrical, with (1−)2−3(−4) distal hila,
7−18(−23) × (2.5−)3−5(−6) μm (av. ± SD: 12.8 ± 3.9 × 3.9 ±
0.7), 0−1(−2)-septate, not constricted at septa, only very slightly
attenuated towards apex and base, base broadly truncate, pale
brown or pale olivaceous-brown, smooth, walls unthickened or
almost so, hila protuberant, denticulate, 0.5−2 μm diam, thickened,
distinctly darkened-refractive; microcyclic conidiogenesis not
observed.
Culture characteristics: Colonies on PDA attaining 8−18 mm diam
after 1 mo, pale olivaceous-grey to olivaceous-grey, sometimes
olivaceous due to abundant sporulation, reverse olivaceous-grey,
velvety to luffy, margin feathery to glabrous, regular, slow growing,
aerial mycelium loose to dense, more abundant at few areas, luffy,
few prominent exudates formed, sporulation profuse. Colonies
on MEA reaching 8−18 mm diam after 1 mo, grey-olivaceous to
olivaceous-grey, whitish to smoke-grey due to aerial mycelium,
reverse olivaceous-grey to iron-grey, velvety to woolly, margin
the genuS Cladosporium
very narrow, white feathery, aerial mycelium dense, high, covering
some parts of the colony, abundant, without conspicuous exudates,
sporulation profuse.
Substrate and distribution: On thalli and apothecia of lichens
(Ascomycota); Asia (Russia, Altai and Taimyr Peninsula), Europe
(Germany, Italy, Norway, Russia) – Cetraria islandica (Germany),
Glypholecia scabra (Norway), Hypogymnia physodes (Germany),
Lecanora chlarotera (Germany), L. carpinea (Germany), Parmelia
sulcata (Germany), Peltigera rufescens (Germany), Pertusaria
alpina (Russia, Altai), Phaeophyscia orbicularis (Germany),
Physcia aipolia (Germany), P. tenella (Germany), Physconia
distorta (Germany), Thamnolia vermicularis [(Russia, Taimyr
Peninsula (Zhurbenko 2012)], Xanthoria parietina (Germany, Italy),
X. polycarpa (Germany).
Additional specimens examined: Germany, Bavaria, Mittelfranken, Kreis ErlangenHöchstadt, car park, BAB Münden-Würzburg behind Nbg. Nord, 49°31'36"N,
10°04'03"E, 310 m NN, on Parmelia sulcata, on Acer platanoides, 23 Jun. 2006,
W. v. Brackel x077; Kreis Kitzingen, Gerentholz, NE Willanzheim, 245 m NN,
49°41'31,8"N, 10°14'09"E, on Physcia tenella, on a fallen twig of Quercus robur,
21 Dec. 2006, W. v. Brackel x117. Italy, Tuscany, Prov. Siena, Siena, between
Belvedere and Tognazza, 335m, on apithecia of Xanthoria parietina, on Fraxinus
ornus, 14 Sep. 2007. W. v. Brackel, IVL 4281 (all collections deposited in the moss
and lichen herbarium of the “Institut für Vegetationskunde und Landschaftsökologie
(IVL)” Hemhofen, Germany). Russia, Taimyr Peninsula, Byrranga Mts., N of
Levinson-Lessinga Lake, 250 m alt., on Thamnolia vermicularis, 20 Aug. 1995, M.
Zhurbenko 95581 (HAL 2444 F, LE 260309).
Notes: Cladosporium licheniphilum, which is a true lichenicolous
species on thalli and apothecia of living lichens, clustered as a
sister to C. phyllophilum (Bensch et al. 2010, ig. 1, part a) and
formed a distinct lineage. Cladosporium antarticum is another
genuine lichenicolous Cladosporium species, but belongs to the
C. herbarum complex. Cladosporium lichenicola is an invalid,
doubtful name, and C. lichenum has been excluded and assigned
to Pseudocercospora (Hawksworth 1979, Heuchert et al. 2005).
Cladosporium licheniphilum is morphologically well distinguishable
from most other species of Cladosporium by having conidiophores
with numerous characteristic terminal branches. Comparable
ramiications are only known in a few other species, e.g. the
uredinicolous North American C. gallicola, and the biotrophic, leafspotting species C. populicola on Populus tremula, C. syringicola
on Syringa ×chinensis and C. ushuwaiensis, known from Argentina
on Berberis ilicifolia, which are, however, easily distinguishable by
various differences in the characters of conidiophores and conidia
(see Schubert 2005b, Schubert & Braun 2006).
91. Cladosporium lineolatum Sacc., Ann. Mycol. 12: 313.
1914. Figs 188, 189.
Lectotype (designated here): Philippines, Prov. Rizal, Alabang,
on leaves of Capparis micracantha (Capparaceae), Mar. 1912,
P.W. Graff, No. 16748 (PAD). Isolectotype: BPI 427242.
Lit.: Saccardo (1931: 789), Schubert (2005b: 95–96).
Ill.: Schubert (2005b: 96, ig. 40, pl. 18, igs A–D).
In vivo: On still living leaves, causing leaf spots or discolorations,
amphigenous, minute, often short striate, blackish, under
stereomicroscope visible as minute spots with pale centre and
distinct, blackish brown margin. Colonies hypophyllous, scattered,
subeffuse, dense, dark brown. Mycelium at irst internal, subcuticular
to intraepidermal, later also external, growing supericially; hyphae
www.studiesinmycology.org
Fig. 188. Cladosporium lineolatum (PAD). Conidiophores and conidia in vivo. Scale
bar = 10 µm. K. Bensch del.
branched, 2–5 µm wide, sometimes swollen, then up to 8 µm
wide, septate, often slightly constricted at the septa, subhyaline
or almost hyaline to pale olivaceous-brown, smooth, walls slightly
thickened. Stromata lacking. Conidiophores solitary or in small,
loose fascicles, arising from internal hyphae, mostly emerging
through stomata, sometimes erumpent through the cuticle, or in
loose to somewhat denser groups, arising from supericial hyphae
as lateral or terminal branches, confusable with hyphae, more or
less erect to subdecumbent, more or less lexuous, iliform, often
mildly geniculate-sinuous, unbranched or branched, sometimes
slightly furcate, 8–70(–90) × 2.5–5(–6) µm, septate, often slightly
constricted at the septa, then cells appearing to be somewhat
swollen, pale to medium olivaceous-brown, somewhat paler towards
the apex, smooth, with age somewhat asperulate, walls thickened,
often two-layered, up to 1 µm wide, sometimes slightly swollen at
the base. Conidiogenous cells integrated, terminal and intercalary,
often somewhat geniculate, 6–15 µm long, proliferation sympodial,
cicatrised, with numerous subdenticulate, protuberant conidiogenous
loci, obconically truncate to slightly convex, 0.5–1.5(–2) µm
diam, thickened, refractive to somewhat darkened. Ramoconidia
sporadically occurring, base broadly truncate, unthickened and only
very slightly attenuated. Conidia in branched chains, subglobose,
ovoid, limoniform, ellipsoid to subcylindrical, 2–14(–16) × 2–4.5 µm,
0–3-septate, mostly not constricted at the septa, subhyaline to pale
olivaceous-brown, smooth or almost so to somewhat irregularly
rough-walled, walls more or less thickened, occasionally apparently
two-layered, apex and base rounded or attenuated, hila protuberant,
obconically truncate to somewhat convex, 0.5–1.5(–2) µm diam,
dome and rim often not very conspicuous, thickened, refractive to
somewhat darkened; microcyclic conidiogenesis occurring.
Substrate and distribution: On Capparis micracantha; Philippines.
Notes: Cladosporium lineolatum is morphologically comparable
with C. myrtacearum, C. cassiae-surathensis and C. chamaeropis.
However, the latter species differs from C. lineolatum in having
somewhat longer and wider conidia, 3–20 × 2–5(–6.5) µm,
wider conidiophores, 4–6.5 µm, usually arranged in moderately
large fascicles and somewhat wider conidiogenous loci and
hila, (0.5–)1–2(–2.5) µm; in C. myrtacearum the conidiophores
are wider, 3–7 µm, and the conidia are longer, above all wider,
3–22 × 2–7 µm, often constricted at the septa; and C. cassiae175
BenSch et al.
Fig. 189. Cladosporium lineolatum (PAD). A. Overview. B. Small fascicle of conidiophores emerging through stomata. C. Conidiophores arising from supericial hyphae. D.
Conidia. Scale bars = 10 (B–D), 20 (A) µm.
surathensis possesses 0–1-septate conidia and non-geniculate
conidiophores. Cladosporium bosciae described on leaves of
Boscia senegalensis, a host belonging to the Capparidaceae, is
quite distinct by forming much longer and wider, 0–6(–8)-septate
conidia, wider conidiophores and wider conidiogenous loci and hila.
92. Cladosporium liriodendri K. Schub. & U. Braun,
Schlechtendalia 14: 71. 2007. Figs 190,191.
Holotype: USA, New York, Tomkins Co., Six Mile Ravine, on
Liriodendron tulipifera (Magnoliaceae), 11 Sep. 1949, C.T.
Rogerson, as “Cercospora or Cladosporium” (NY).
Lit.: Schubert (2005b: 96–98).
Ill.: Schubert (2005b: 97, ig. 41, pl. 18, igs E–I), Schubert & Braun
(2006: 72, ig. 8, pl. 2, igs A–C).
In vivo: On living leaves, leaf spots amphigenous, subcircular,
but more or less irregular in outline, 2–10 mm wide, sometimes
somewhat extended, up to 14 mm long, medium to dark brown
or yellowish brown, ochraceous, sometimes greyish brown in the
centre, surrounded by a narrow, medium to dark brown, irregular
margin, on the lower leaf surface somewhat paler, affected areas
inally dropping out, forming shot holes symptoms. Colonies
Fig. 190. Cladosporium liriodendri (NY). Symptoms, conidiophores and conidia in
vivo. Scale bar = 10 µm. K. Bensch del.
176
the genuS Cladosporium
Fig. 191. Cladosporium liriodendri (NY). A. Conidiophore with coronate scars. B. Overview. C. Conidium showing coronate scar structure. D. Tips of conidiophores with several
conspicuous, somewhat darkened conidiogenous loci. E. Conidia. Scale bars = 5 (A, C), 10 (D–E), 20 (B) µm.
amphigenous, loosely scattered, villose, not caespitose, brown.
Mycelium internal, subcuticular to intraepidermal, hyphae sparingly
branched, 4.5–6 µm wide, septate, pale to mostly medium brown,
smooth, walls slightly thickened, often swollen. Stromata or stromatic
hyphal aggregations usually small, subglobose to somewhat oval,
15–40 µm diam., sometimes larger, composed of large, swollen
hyphal cells, subglobose to angular-oblong, 8–13(–20) µm wide,
dark brown, thick-walled. Conidiophores solitary or in small loose
groups, mostly in pairs or up to three, arising from swollen hyphal
cells or stromatic hyphal aggregations, erumpent through the
cuticle, erect, straight to slightly lexuous, not to only somewhat
geniculate-sinuous, unbranched, rarely branched, 45–200 × 4–7(–8)
µm, pluriseptate, medium to dark brown, smooth, walls distinctly
thickened, sometimes two-layered, up to 1 µm wide, often swollen
at the base and somewhat attenuated towards the apex, sporadically
subnodulose, swellings sometimes with conidiogenous loci, but
loci not restricted to them. Conidiogenous cells integrated, terminal
and intercalary, 8–39 µm long, proliferation sympodial, with few
protuberant conidiogenous loci, 1–2.5(–3) µm diam, conspicuously
differentiated in a central dome and a raised periclinal rim, thickened,
only somewhat darkened-refractive. Conidia in unbranched or
branched chains, straight, obovoid, ellipsoid to subcylindrical,
5–15(–20) × (2.5–)3.5–6(–7) µm, 0–1(–3)-septate, not constricted
at the septa, in 1-septate conidia the septum more or less median,
pale to medium brown, smooth, walls more or less thickened, apex
rounded, slightly attenuated or truncate, hila protuberant, 1–2.5 µm
diam, thickened, only somewhat darkened-refractive; occasionally
microcyclic conidiogenesis occurring.
www.studiesinmycology.org
Substrate and distribution: On Liriodendron tulipifera; USA.
Notes: This species is similar to C. cladosporioides, which differs,
however, in having narrower conidiophores, 2.5–5 µm wide, with
usually one-layered walls and longer, somewhat narrower conidia,
5–30 × 3–4(–5) µm. Cladosporium galii is also morphologically
close to C. liriodendri, but the conidia are longer, 6–30(–40) µm,
and, above all, minutely verruculose (Mułenko et al. 2004). Farr et
al. (1989) recorded C. cladosporioides on Liriodendron in the USA,
which possibly refers to C. liriodendri.
93. Cladosporium lupiniphilum U. Braun, A monograph of
Cercosporella, Ramularia and allied genera (phytopathogenic
hyphomycetes) 2: 410. 1998. Fig. 192.
Holotype: Byelorussia, Minsk area, on Lupinus luteus (Fabaceae),
1959, (?) Chenashskaya (LEP, as “Ramularia lupini”).
Lit.: Schubert (2005b: 98–99).
Ill.: Braun (1998: 411, ig. 664), Schubert (2005b: 99, ig. 42).
In vivo: Leaf spots amphigenous, subcircular to irregular, 1–3 mm
wide, pale yellowish ochraceous to greyish white, margin indeinite
or with a narrow darker marginal line. Colonies amphigenous, mostly
hypophyllous, subeffuse, pale brownish. Mycelium internal; hyphae
branched, 1.5–5 µm wide, septate, hyaline, subhyaline to pale
yellowish olivaceous or olivaceous-brown, forming small stromatic
177
BenSch et al.
Fig. 192. Cladosporium lupiniphilum (LEP). Conidiophores and conidia in vivo. Scale
bar = 10 µm. U. Braun del.
hyphal aggregations, up to 10 µm wide, subglobose, subcuticular
to intraepidermal, rarely substomatal, often conluent, sometimes
almost crustose. Conidiophores solitary or in small, loose fascicles,
arising from internal cells or stromatic hyphal aggregations,
erumpent through the cuticle, rarely emerging through stomata,
erect, subcylindrical, hardly geniculate-sinuous, non-nodulose,
unbranched, 20–80 × 3–8 µm, continuous or sparingly septate,
subhyaline, pale olivaceous to yellowish brown, smooth, walls
only slightly thickened. Conidiogenous cells integrated, terminal,
with few conidiogenous loci, prominent, 1–2 µm diam, thickened
and darkened. Conidia catenate, usually in branched chains,
ellipsoid-ovoid (-subglobose), subcylindrical-fusiform, 6–28 × 4–8
µm, 0–3-septate, subhyaline, pale olivaceous to yellowish brown,
smooth or almost so, ends somewhat attenuated, hila prominent,
1.5–2.5 µm diam, thickened and darkened.
Fig. 193. Cladosporium lycoperdinum (K 121561). Conidiophores in loose fascicles
arising from stromata and conidia in vivo. Scale bar = 10 µm. B. Heuchert del.
Substrate and distribution: On Lupinus luteus; Byelorussia.
Notes: Cladosporium lupiniphilum is easily distinguishable from
morphologically similar Cladosporium species with relatively short,
loosely fasciculate conidiophores by having smooth conidia [faintly to
conspicuously verruculose-echinulate in C. praecox], wider, 0–3-septate
conidia [2–5 µm wide, 0–1-septate in C. cheonis; 2–5(–6.5) µm wide,
0–1(–3)-septate in C. chamaeropis], and wider conidiogenous loci and
hila [1–1.5 µm diam in C. cheonis], respectively.
94. Cladosporium lycoperdinum Cooke, Grevillea 12(61):
32. 1883. Figs 193–195.
Lectotype (designated in Heuchert et al. 2005): USA, South
Carolina, Aiken, on Lycoperdon sp. (Agaricales), Ravenel & Cooke,
Fungi Amer. Exs. 595 (K 121561). Isolectotypes: Ravenel & Cooke,
Fungi Amer. Exs. 595 (e.g., BPI 427244, NY).
178
Fig. 194. Cladosporium lycoperdinum (from B 700006387 and a collection from PH).
Conidiophores arising from swollen hyphal cells, ramoconidia and conidia in vivo.
Scale bar = 10 µm. B. Heuchert del.
the genuS Cladosporium
Fig. 195. Cladosporium lycoperdinum (CBS 574.78c). A–D. Macronematous conidiophores and conidial chains in vitro. Scale bar = 10 µm.
Lit.: Saccardo (1886: 368), Heuchert et al. (2005: 33–36), Bensch
et al. (2010: 58–60).
Ill.: Heuchert et al. (2005: 34–35, igs 11–12), Bensch et al. (2010:
59, ig. 48).
Exs.: Herter, Plantae Urug. Exs. 1452; Sydow, Mycoth. Germ. 1781.
In vivo: Colonies olivaceous to dark brown, effuse, loose to dense,
caespitose, velvety. Mycelium external, supericial; hyphae branched,
3–12 µm wide, cells 5–10 µm long, often subglobose, angular to
irregularly swollen by constrictions at the septa, swollen cells sometimes
aggregated, pale olivaceous to olivaceous-brown, occasionally
subhyaline, smooth. Stromata effuse, composed of swollen hyphal
cells, 5–12 µm diam., dark olivaceous-brown or medium brown,
smooth, walls thickened. Conidiophores solitary, arising from swollen
hyphal cells, or in loose to dense fascicles, arising from stromata, erect
to decumbent, straight to curved, subcylindrical, slightly geniculatesinuous, unbranched to branched, 39–265 × 3–6(–8) µm, 6–9 µm
wide at the base, occasionally somewhat attenuated towards the apex
(up to 3.5 µm), 4–12-septate, at the base septa often closely spaced,
occasionally slightly constricted at the septa, pale to medium olivaceous,
sometimes paler towards the tips, occasionally subhyaline, smooth
or almost so, walls slightly thickened, thin-walled towards the apex,
rarely enteroblastically proliferating, monopodial. Conidiogenous cells
integrated, terminal or intercalary, cylindrical, 8–53 µm long, polyblastic,
proliferation sympodial, with up to four not or only slightly protuberant
conidiogenous loci, only occasionally denticle-like or situated on small
lateral shoulders, thickened and darkened-refractive, 1–2(–3) µm
diam, intercalary conidiogenous cells sometimes somewhat swollen.
Ramoconidia occasionally formed, subcylindrical to ampulliform, 21–
32 × 4.5–5.5 µm, 0–5-septate, usually not constricted at the septa,
smooth or rarely irregularly minutely verruculose, walls thickened, base
convex without a cladosporioid hilum, 2–3 µm wide, apex with up to
ive hila. Conidia usually in branched chains, straight, rarely curved,
variable in shape and size, fusiform, obovoid, limoniform, narrowly to
broadly ellipsoid, (2–)6–28 × 2.5–7(–10) µm, 0–3-septate, without any
constrictions, pale olivaceous, occasionally subhyaline, smooth, walls
unthickened or almost so, apex rounded, with up to four hila, base
truncate to convex, sometimes attenuated, hila slightly protuberant,
denticle-like, thickened, darkened-refractive, (0.5–)1–2(–2.5) µm diam;
microcyclic conidiogenesis observed.
www.studiesinmycology.org
In vitro: Mycelium unbranched or loosely branched, iliform to
cylindrical-oblong, (0.5−)1−5 μm wide, not constricted at septa,
subhyaline to pale or medium olivaceous-brown, smooth or almost so
to often minutely verruculose or loosely verrucose, walls unthickened
or almost so, occasionally forming ropes. Conidiophores macro- and
micronematous, solitary, arising terminally and laterally from hyphae,
erect, straight or slightly lexuous, macronematous conidiophores
cylindrical-oblong or iliform, non-nodulose, usually not geniculate,
occasionally slightly geniculate at or towards the apex due to
sympodial proliferation, unbranched or once, rarely twice branched,
branches often only as short lateral peg-like prolongations just below
a septum, 20−250 × (2.5−)3−6(−6.5) μm, pluriseptate, with septa
occasionally in short succession, not constricted at septa, few septa
sometimes darkened just below potential ramoconidia or where
conidiophores disarticulate into shorter pieces, pale olivaceous to
medium olivaceous-brown, smooth to somewhat irregularly roughwalled or minutely verruculose, especially at or towards the base,
walls unthickened or almost so, about 0.5 μm wide, sometimes
slightly attenuated towards the apex or intercalary somewhat wider;
micronematous conidiophores narrower, shorter and paler, 9−105 ×
1.5−2.5 μm, iliform, not geniculate, unbranched or once branched,
0−5-septate, subhyaline to pale olivaceous, conidiogenous cells
6.5−50 μm long, loci 0.5−1.2 μm diam. Conidiogenous cells
integrated, terminal, intercalary or sometimes pleurogenous, often
seceding and forming ramoconidia, cylindrical-oblong, sometimes
slightly geniculate due to sympodial proliferation, 10−57 μm long,
with (1−)2−4 loci at or towards the apex, sometimes with additional
loci situated on a lower level, in intercalary conidiogenous cells loci
usually situated on small peg-like lateral outgrowths, loci conspicuous,
subdenticulate to denticulate, 1−2 μm diam, thickened and darkenedrefractive. Ramoconidia often formed, cylindrical-oblong, 13.5−55 ×
3−5(−5.5) μm, 0−3(−6)-septate, not constricted at septa, with 2−4
distal hila, base broadly truncate, 2.2−3(−3.5) μm wide, unthickened
or slightly thickened, often somewhat darkened or refractive, without
dome and rim. Conidia catenate, in branched chains branching in
all directions, up to 5(−7) conidia in the terminal unbranched part
of the conidial chains, straight, small terminal conidia subglobose
to obovoid or narrowly ellipsoid, (2−)3.5−5 × (1.5−)2−2.5(−3) μm
(av. ± SD: 4.2 ± 0.7 × 2.0 ± 0.3), aseptate, intercalary conidia
limoniform, ovoid to ellipsoid, 4−14(−16.5) × (2−)2.5−3(−4) μm (av.
179
BenSch et al.
± SD: 8.6 ± 3.0 × 2.8 ± 0.5), 0(−1)-septate, with 1−3(−4) distal hila,
secondary ramoconidia ellipsoid to cylindrical, sometimes almost
doliiform, 8−32(−38) × (2.5−)3−4(−5) μm (av. ± SD: 15.6 ± 6.3 × 3.5
± 0.5), 0−1(−3)-septate, not constricted at septa, pale olivaceous
to pale olivaceous-brown, smooth or almost so, walls unthickened
or almost so, with 2−5 distal hila, intercalary conidia and secondary
ramoconidia sometimes formed in dense whirls at the conidiogenous
cells or secondary ramoconidia, hila conspicuous, subdenticulate,
0.5−2(−2.5) μm diam, thickened and darkened-refractive; microcyclic
conidiogenesis occasionally occurring.
Culture characteristics: Colonies on PDA attaining 50−68 mm
diam after 14 d, olivaceous-grey, grey-olivaceous towards
margins, reverse leaden-grey to olivaceous-black, loccose to
luffy, margins white to grey-olivaceous, feathery, regular, aerial
mycelium abundant, covering the whole colony surface, loccose
to luffy, growth lat to low convex, without prominent exudates,
sporulation profuse. Colonies on MEA reaching 50−62 mm diam
after 14 d, olivaceous-grey to pale olivaceous-grey, sometimes
smoke-grey or white, reverse olivaceous-grey to iron-grey, loccose
to felty, margins white, narrow, feathery, regular, aerial mycelium
abundant, covering the whole colony surface, growth lat to low
convex, sometimes radially furrowed, without prominent exudates,
sporulation profuse. Colonies on OA attaining 58−70 mm diam
after 14 d, olivaceous to greenish olivaceous, olivaceous-grey at
margins, reverse leaden-grey to olivaceous-grey, loccose to felty,
margins glabrous, aerial mycelium abundant covering almost the
whole colony surface, loose to dense, low to rarely high, growth lat,
without prominent exudates, sporulation profuse.
Substrates and distribution: On ascomycetes and fruiting bodies of
different basidiomycetous fungi; Europe (Germany, Russia), North
America (Canada, USA) and South America (Colombia, Uruguay) –
Apiosporina morbosa (Canada), Aureobasidium caulivorum (Russia),
Gomphidius viscidus (Germany), Lactarius volemus (USA, NY),
Lepiota procera (Germany), Lycoperdon sp. (USA, SC), Phyllactinia
guttata (USA, WA), Piptoporus betulinus (Germany), Polyporus sp.
(USA, DE, NY), Scleroderma tuberoideum (South America, Uruguay),
on Suillus bovinus (Germany), unknown ascomycete (USA, WA).
Additional specimens examined: Canada, Ontario, isol. from galls of Apiosporina
morbosa (Venturiaceae) on Prunus sp. (Rosaceae), 2005, coll. K.A. Seifert, isol.
P.W. Crous (CBS 126347 = CPC 12102). Colombia, near Cogna, bosque andino,
ca. 3000 m alt., isol. from Puya sp. (Bromeliaceae), W. Gams, depos. May 1980,
isol. by W. Gams (CBS 274.80C). Germany, Brandenburg, (probably Triglitz),
on Lepiota procera, 4 Oct. 1905, O. Jaap, Fl. Prov. Br. 102 (B 700006246), as
C. fuligineum [later revised by Braun (2001) as C. diaphanum, mixed infection
together with C. herbarum]; Kreis Nieder-Barnim, forest between Sophienstädt and
Ruhlsdorf, on decaying gills of Boletus bovinus (= Suillus bovinus), 7 Nov. 1917, P.
Sydow, Sydow, Mycoth. Germ. 1781 (HBG), as C. fuligineum [mixed collection with
C. episclerotiale and C. herbarum]; Kreis Prignitz, Triglitz, on Gomphidius viscidus,
5 Oct. 1905, O. Jaap, Fl. Prig. 74 (B 700006245), as C. fuligineum [later re-identiied
by Braun (2001) as C. diaphanum Thüm.; mixed collection with C. herbarum];
Nordrhein-Westfalen, Kreis Olpe, near Silberg, Sellenbruch, on Polyporus betulinus
(≡ Piptoporus betulinus), 20 Jun. 1947, A. Ludwig (B 700006387), as C. fuligineum.
Russia, Moscow region, isol. from Aureobasidium caulivorum (Dothioraceae),
ident. by W. Gams, stored as “C. epichloës” (CBS 574.78C = VKM F-2759).
Uruguay, Dep. de San José, on Scleroderma tuberoideum, 5 Oct. 1939, W.G.
Herter, Herter, Plantae Urug. Exs. 1452 (NY), as C. epimyces. USA, Delaware, on
Polyporus sp., without date, A. Commons (PH); Wilmington, on Polyporus sp., 26
Oct. 1891, herb. A. Commons Nr. 1879 (PH), as C. epimyces; Illinous, Lake Forest,
on an old Polyporus sp., 16 Oct. 1892, R.A. Harper (NY); New York, Newield, on
Lactarius volemus, Sep. 1897 (NY), as C. epimyces; Washington, Metalline Falls,
on an unknown ascomycete on Abies grandis, 31 Aug. 1930, G.G. Hedgcock (BPI
427384), as C. peridermiicola in herb.; Seattle, University of Washington campus,
isol. from chasmothecia of Phyllactinia guttata (Erysiphales) on leaves of Corylus
avellana (Betulaceae), 12 Feb. 2004, D. Glawe (CBS 126348 = CPC 11833).
180
Notes: This species can be classiied as fungicolous
hyphomycete being conined to fruiting bodies of ascomycetes
and basidiomycetes. Based on the collections examined it can
be suggested that this species, which was until the treatment in
Heuchert et al. (2005) only known from the type collections, is
probably widespread and common. An epitype has not yet been
designated since type material was collected on a basidiomycete,
but the available cultures, which morphologically coincide with C.
lycoperdinum (Heuchert et al. 2005), are isolated from ascomycetes
(Bensch et al. 2010). A collection of “Cladosporium epimyces”
on Scleroderma tuberoideum (NY), overgrowing basidiospores,
is tentatively assigned to C. lycoperdinum, although a few
conidiophores with swellings (up to 10 µm) have been observed.
“Peridermium columnare” (BPI 427384) [deposited under “C.
peridermiicola” in herb.], inhabiting stromata of an unknown
ascomycete, is morphologically also indistinguishable from C.
lycoperdinum. In a specimen on Lactarius volemus (NY), some
broadly ellipsoid to subcylindrical, darker conidia, 13–20 × 5.5–8.5
µm, 1–3-septate, with constrictions at the septa, have been seen,
but it is not clear if they belonged to C. lycoperdinum or if a mixed
infection was involved. Cladosporium epimyces, also occurring
on fungal fruiting bodies, possesses much larger, subcylindrical
ramoconidia, 33–53 × 4.5–6 µm, 0–6-septate. The conidiophores
are, in comparison with C. lycoperdinum, frequently dichotomously
to irregularly branched (Heuchert et al. 2005).
95. Cladosporium macrocarpum Preuss, in Sturm,
Deutsch. Fl. 3(26): 27. 1848, nom. cons. prop. Figs 196–199.
≡ Cladosporium herbarum var. macrocarpum (Preuss) M.H.M. Ho &
Dugan, Mycotaxon 72: 131. 1999.
Neotype (designated by Schubert et al. 2007b): USA, Washington,
isolated from Spinacia oleracea (Chenopodiaceae), 1 Jan. 2003,
L. DuToit (CBS H-19855). Isoneotype: HAL 2020 F. Ex-neotype
culture: CBS 121623 = CPC 12755.
= Dematium herbarum var. (β) brassicae Pers., Syn. meth. fung. 2: 699. 1801
[holotype: L 910.255-736 = L-0115836].
= Dematium graminum Pers., Mycol. eur. 1: 16. 1822 [lectotype: L 910.255723 = L-0115836].
Fig. 196. Cladosporium macrocarpum (CBS 299.67). Macro- and micronematous
conidiophores and conidia in vitro. Scale bar = 10 µm. K. Bensch del.
the genuS Cladosporium
Fig. 197. Cladosporium macrocarpum (CBS 299.67), anamorphic and teleomorphic state (CPC 12755). A–C. Macronematous conidiophores and conidia. D–G. Micronematous
conidiophores. H. Microcyclic conidiogenesis. I. Ascomata formed on nettle stems in culture. J. Periphyses. K, M–N. Asci. L. Ostiole. Scale bars = 10 (A, D–H, J–N), 200 (I) µm.
≡ Cladosporium graminum (Pers.) Link, in Willd., Sp. pl. 6(1): 42. 1824.
≡ Chloridium graminum (Pers.) Chevall., Fl. gén. env. Paris 1: 36. 1826.
= Dematium vulgare var. (β) foliorum Pers., Mycol. eur. 1: 14. 1822 [holotype:
L 910.255-736 = L-0115871].
≡ Cladosporium herbarum α foliorum (Pers.) Chevall., Fl. gén. env. Paris
1: 36. 1826.
= Dematium vulgare var. (δ) typharum Pers., Mycol. eur. 1: 14. 1822 [lectotype:
L 910.255-698 = L-0115852].
= Cladosporium brunneum Corda, Icon. fung. 1: 15. 1837 [holotype: PRM
657440].
= Cladosporium gracile Corda, Icon. fung. 1: 15. 1837 [holotype: PRM
155423].
≡ Didymotrichum gracile (Corda) Bonord., Handb. Mykol.: 89. 1851.
= Cladosporium lanciforme Ces., Flora 36: 204. 1853 [syntypes: Rabenh.,
Herb. Viv. Mycol. 1788, e.g. B; HAL; HBG; LE 160383, 160385].
www.studiesinmycology.org
≡ Cladosporium typharum f. lanciforme (Ces.) Ferraris, Flora Ital. Crypt.,
Pars I, Fungi, Fasc. 8: 337. 1912.
= Cladosporium cardariae Opiz, Lotos 5: 41. 1855 [syntypes: PRM 657446,
657447].
= Cladosporium herbarum f. lignicola Thüm., Fungi Austr. Exs., Fasc. 9, No.
891. 1874 [syntypes: Thümen, Fungi Austr. Exs., e.g., BPI, HAL].
= Cladosporium kniphoiae Cooke, Grevillea 14(70): 40. 1885 [holotype: K
121560].
= Heterosporium minutulum Cooke & Massee, Grevillea 16(77): 11. 1887
[holotype: K].
= Cladosporium algarum Cooke & Massee, Grevillea 16(79): 80. 1888
[lectotype: K, isolectotype: NY, designated by Kohlmeyer].
≡ Heterosporium algarum (Cooke & Massee) Cooke & Massee, in Cooke,
Grevillea 18(88): 74. 1890.
= Heterosporium typharum Cooke & Massee, Grevillea 16(79): 80. 1888
181
BenSch et al.
Fig. 198. Cladosporium macrocarpum (CBS 299.67). A. Survey of a conidiophore that forms several secondary ramoconidia and conidia. Several aerial hyphae are also
visible in this picture. B. Conidiophore with broadly ellipsoid secondary ramoconidia and obovoid conidia. Note the different scars on the conidiophore at the lower left. C.
Ellipsoid or obovoid conidia with notable areas of scar formation. The ornamentation is relatively widely distributed over the body of the cell and similar to C. variabile. D. Detail
of a conidiophore (see B) with scars. Note the relatively shallow rings of the scars. E. Details of conidia and a secondary ramoconidium. F. Conidiophore with a secondary
ramoconidium and conidia. Note the hila on several spores and the lack of ornamentation at the site where spores are formed. Scale bars = 2 (E), 5 (D, F), 10 (A–C) µm.
[lectotype: NY, designated by J. David].
= Heterosporium phragmitis var. typharum Cooke, Grevillea 16(80): 109.
1888, nom. inval. [type: K].
= Heterosporium maculatum Klotzsch ex Cooke, Grevillea 17(83): 65. 1888
[lectotype: K, designated by J. David].
= Heterosporium fungicola Ellis & Everh., J. Mycol. 5: 70. 1889, as “fungicolum”
[type not preserved; neotype (designated here): USA, Nebrasca, on Polyporus
182
pricipes, Lincoln, 1888, R. Pound, NY].
= Heterosporium hybridum Ellis & Everh., J. Mycol. 5: 70. 1889 [holotype: NY].
= Heterosporium cleomis Ellis & Everh., in Kelsey, J. Mycol. 5: 82. 1889, nom.
inval.
= Fusicladium destruens Peck, Rep. (Annual) New York State Mus. Nat. Hist.
43: 30. 1890 [holotype: NYS].
= Cladosporium phoenicis Roum., Fungi Sel. Gall. Exs. , Cent. 58, No. 5798.
the genuS Cladosporium
= Heterosporium tortuoso-inlatum Bubák, Bot. Közlem. 15(3–4): 82. 1915
[holotype: BPI].
= Cladosporium alnicola Bubák & Vleugel, Svensk Bot. Tidskr. 11: 322. 1917,
nom. illeg., non C. alnicola Corda, 1837 [holotype: BPI 426104].
= Cladosporium epiphyllum var. acerinum Sacc., Nuovo Giorn. Bot. Ital., N.S.,
27: 86. 1920 [holotype: PAD].
= Heterosporium laricinum Dearn., Mycologia 21: 328. 1929 [holotype: DAOM
6812].
= Heterosporium maydis Lobik, Trudy Severo-Kavkazsk. Inst. Zashch.
Rast. 1(2): 42. 1933 [neotype (designated here): Russia, Leningrad Oblast,
Siverskaya, on Zea mays, 8 Sep. 1955, E.g. Potatosova (LEP)].
= Heterosporium thapsiae Petr., Denkschr. Akad. Wiss. Wien 105(2): 26. 1943
[holotype: W 08842].
= Heterosporium petuniae R. Sprague, Mycologia 57: 658. 1965 [holotype:
WSP 46160].
= Davidiella macrocarpa Crous, K. Schub. & U. Braun, Stud. Mycol. 58: 129.
2007.
= Cladosporium equiseti Pass., in herb. [B 700006324].
= Heterosporium cerastis Jacz., in herb. [LEP].
= Heterosporium trichostematis Jacz., in herb. [LE 41142].
Fig. 199. Cladosporium macrocarpum (CPC 12755, neotype). Ascus and
ascospores. Scale bar = 10 µm. P.W. Crous del.
1891 and Rev. Mycol. (Toulouse) 13: 133. 1891 [lectotype (designated here):
B 700006677. Isolectotypes: Roumeguère, Fungi Sel. Gall. Exs. 5798, e.g. FH,
etc.].
= Cladosporium myriosporum Ellis & Dearn., Proc. Canad. Inst., N.S., 3, 1: 90.
1897 [syntypes: DAOM, NYS].
= Heterosporium tuberculans Ellis & Everh., Erythea 1: 203. 1893 [syntypes:
Ellis & Everhart, N. Amer. Fungi 3295, e.g., NY, K].
= Heterosporium sphaeriiforme Ellis & Everh., Proc. Acad. Nat. Sci.
Philadelphia 46: 381. 1894, as “sphaeriaeforme” [holotype: NY].
= Cladosporium menispermi Allesch., Hedwigia 34: 220. 1895 [holotype:
M-0057686].
= Heterosporium proteus Starbäck, Bot. Centralbl. 64: 382. 1895 [syntypes:
Eriksson, Fungi Paras. Scand. Exs. 500, e.g. K].
= Cladosporium idesiae Bres., Hedwigia 35(1), Beih.: 62. 1896 [type: B
70000655].
= Heterosporium oxybaphi F. Patt., Bull. Torrey Bot. Club 27: 285. 1900 [type:
BPI].
= Heterosporium calandriniae Massee, Bull. Misc. Inform. 175–177: 168. 1901
[holotype: K].
= Heterosporium chamaeropis Oudem., Beih. Bot. Centralbl. 11: 539. 1902
[holotype: L].
= Heterosporium hordei Bubák, Sber. K. böhm. Ges. Wiss., Math.-Nat. Kl.
1903(12): 20. 1903 [holotype: BPI].
= Cladosporium magnoliae Lindau, Verh. Bot. Vereins Prov. Brandenburg 47:
74. 1905 [holotype: B].
= Heterosporium amsoniae Kabát & Bubák, Hedwigia 47: 362. 1908 [holotype:
BPI].
www.studiesinmycology.org
Lit.: Saccardo (1886: 352), Lindau (1907: 805), Ferraris (1912: 335),
Gonzáles-Fragoso (1927: 200), de Vries (1952: 76), Ellis (1971:
315), Shvartsman et al. (1975: 97–111), Domsch et al. (1980: 208),
Ellis & Ellis (1985: 290, 468), Matsushima (1985: 5), McKemy &
Morgan-Jones (1991c), Dugan & Roberts (1994), David (1997: 71),
Samson et al. (2000: 112), Schubert et al. (2007b: 129–133).
Ill.: Preuss (1848: Tab. 14), Gonzáles-Fragoso (1927: 200, Fig. 44),
de Vries (1952: 77–78, Figs 16–17), Ellis (1971: 314, Fig. 217 C),
Shvartsman et al. (1975: 98–99, Figs 57–58), Domsch et al. (1980:
209, Fig. 84), Matsushima (1985: 58, Fig. 311), Dugan & Roberts
(1994: 516, Figs 1–3), David (1997: 62, Fig. 17 H–I; 89, Fig. 22
G–H), Samson et al. (2000: 112, Fig. 50; 113, Pl. 48), Schubert et
al. (2007b: 129–132, Figs 22–25).
Exs.: Allescher & Schnabl, Fungi Bav. 592, 593; Ellis, N. Amer.
Fungi 650; Fl. Hung. Exs. 206; Fuckel, Fung. Rhen. Exs. 109;
Kabát & Bubák, Fungi Imp. Exs. 795; Krieger, Fungi Saxon. Exs.
2091 p.p.; Liro, Mycoth. Fenn. 829; Petrak, Fl. Bohem. Morav. Exs.
Pilze 513 b, 557; Petrak, Mycoth. Gen. 215; Roumeguère, Fungi
Sel. Gall. Exs. 948, 1060, 1159, 1365, 3294, 3594, 5798; Smarods,
Fungi Lat. Exs. 650; Sydow, Mycoth. Germ. 2044; Sydow, Mycoth.
March. 485, 899, 4498; Thümen, Fungi Austr. Exs. 535; Vestergren,
Micromyc. Rar. Sel. Praec. Scand. 1798.
Ascomata supericial on a small stroma, black, up to 200 μm
diam, globose, separate, but developing with 1–3 necks with age;
ostioles consisting of pale brown to subhyaline cells, periphysate,
with periphysoids growing into the cavity; wall consisting of 3–6
layers of medium brown textura angularis. Pseudoparaphyses
present, hyaline, subcylindrical, septate, anastomosing, 3–4
μm diam; hamathecial cells persistent in cavity. Asci fasciculate,
bitunicate, subsessile, broadly ellipsoid with a long tapered stalk,
straight to curved, 8-spored, 70–110 × 15–20 μm. Ascospores tri- to
multiseriate, overlapping, hyaline, guttulate, irregular lumina rarely
observed, thick-walled, straight to slightly curved, fusoidellipsoidal
with obtuse ends, widest in the middle of the apical cell, medianly
1-septate, not to slightly constricted at the septum, tapering
towards both ends, but more prominently towards lower end, (22–)
23–26(–28) × (6–)6.5–7(–8) μm; mucoid sheath rarely observed,
mostly absent.
In vivo: Colonies on dead leaves, stems and other organic substrates
rather inconspicuous to conspicuous, caespitose, effuse, punctiform
to almost pustulate, dark to blackish olivaceous or olivaceous-brown,
velvety. Mycelium usually immersed, rarely with few supericial
183
BenSch et al.
hyphae. Stromata usually lacking, occasionally with loose stromatic
hyphal aggregations. Conidiophores solitary or in small to large
facicles, loose to moderately dense, large fascicles occasionally
in almost pustulate aggregations, arising from internal hyphae or
swollen hyphal cells, erect, usually distinctly nodulose-nodose to
geniculate-nodulose, up to 8(–10) µm wide (otherwise agreeing with
the conidiophores formed in vitro, see below). Conidia often distinctly
dimorphic, with smaller conidia formed in simple or branched chains,
8–20 × 5–9 µm, and some larger, above all broader conidia, 15–25(–
30) × (6–)7–12(–15) µm, formed singly or in short simple to branched
chains, micronematous conidiophores not formed in vivo (otherwise
agreeing with conidia formed in vitro, see below).
In vitro: Mycelium unbranched or loosely branched, 1–4.5(–5) μm
wide, septate, sometimes slightly constricted at septa, hyaline to
pale brown, smooth to minutely verruculose, walls unthickened
or slightly thickened. Conidiophores micronematous and
macronematous, solitary, arising terminally from plagiotropous
hyphae or terminally from ascending hyphae. Macronematous
conidiophores erect, straight to somewhat lexuous, cylindricaloblong, nodulose to nodose, with a single apical or usually several
swellings either somewhat distinct from each other or often in
short succession giving conidiophores a knotty appearance,
swellings sometimes laterally elongated or formed at the top of
a branch-like outgrowth below the apical swelling, sometimes
distinctly geniculate, unbranched, sometimes branched, 12–260
× (3–)4–6 μm, swellings 5–10 μm wide, pluriseptate, sometimes
slightly constricted at septa, pale to medium brown or olivaceousbrown, somewhat paler at apices, smooth to minutely verruculose
or verruculose, walls somewhat thickened, sometimes even twolayered. Conidiogenous cells integrated, terminal or intercalary,
cylindrical, nodulose with lateral shoulders or nodose with swellings
round about the stalk, with conidiogenous loci conined to swellings,
12–37 μm long, with up to 12 loci per cell, usually with up to six, loci
conspicuous, protuberant, (1–)1.5–2 μm diam, somewhat thickened
and darkened-refractive. Micronematus conidiophores almost
indistinguishable from hyphae, straight, narrowly iliform, nonnodulose or with a single or few swellings, mostly with small headlike swollen apices, usually only few micrometer long, 1.5–3 μm
wide, aseptate or with only few septa, subhyaline, smooth or almost
so, walls unthickened, with a single or only few conidiogenous loci,
narrow, 0.8–1.2 μm diam, thickened and somewhat darkenedrefractive. Conidia catenate, in branched chains, small terminal
conidia subglobose, obovoid, oval, limoniform, 4–11 × (3–)4–6 μm
[av. ± SD, 7.6 (± 1.9) × 5.0 (± 0.8) μm], aseptate, intercalary conidia
broadly ovoid-ellipsoid, 10–17 × (4.5–)5–9 μm [av. ± SD, 12.7 (±
2.1) × 6.8 (± 0.8) μm], 0–1-septate; secondary ramoconidia broadly
ellipsoid to subcylindrical, 14–25(–30) × (5–)6–9(–10) μm [av. ±
SD, 19.4 (± 3.5) × 7.6 (± 1.0) μm], 0–2(–3)-septate, sometimes
slightly constricted at the septa, septa somewhat sinuous with age,
pale brown to medium olivaceous-brown or brown, sometimes
even dark brown, verruculose to echinulate (muricate under
SEM), walls thickened, up to 1 μm thick, mostly broadly rounded
at apex and base, sometimes attenuated, sometimes guttulate
by oil drops, with up to three apical hila, mostly 1–2, hila sessile
(apparently somewhat immersed) to somewhat protuberant,
1–2(–2.5) μm diam, thickened and darkened-refractive; microcyclic
conidiogenesis occurring with conidia forming secondary microand macronematous conidiophores, conidia often germinating with
long hyphae. Conidia formed by micronematous conidiophores
usually smaller, narrower and paler, catenate, in short unbranched
or branched chains, subglobose, obovoid to limoniform, ellipsoid
184
or fusiform, 2.5–16 × 1.5–5 μm, 0(–1)-septate, few longer conidia
subcylindrical to clavate, up to 37(–43) μm long, 0–2(–3)-septate,
occasionally with up to four septa, sometimes slightly constricted
at the septa, subhyaline to pale brown, almost smooth to minutely
verruculose, walls unthickened, hila 0.8–1.2 μm diam, thickened
and darkened-refractive.
Culture characteristics: Colonies on PDA reaching 30–43 mm
in diam after 14 d at 25 ºC, dark dull green to olivaceous-grey,
olivaceous-grey, dark olivaceous- to iron-grey reverse, pulvinate,
velvety, sometimes somewhat zonate, paler zones towards the
margin, margin regular, entire edge, almost colourless to white,
glabrous to feathery, aerial mycelium sparse to more abundant
in the colony centre or covering large areas of the colony, hairy,
luffy or felty, whitish to smoke-grey, sometimes becoming reddish,
livid red to vinaceous, growth lat, regular, sometimes forming
few prominent exudates, exudates sometimes slightly purplish,
sporulation profuse with two kinds of conidiophores, low and high.
Colonies on MEA reaching 31–50 mm in diam after 14 d at 25
ºC, grey-olivaceous to olivaceous-grey or iron-grey, sometimes
pale olivaceous-grey to whitish due to abundant aerial mycelium,
olivaceous-grey or iron-grey reverse, velvety or powdery, margin
narrow, entire edge, colourless to white, glabrous, aerial mycelium
sparse to abundant, hairy or felty, growth regular, lat to low convex,
radially furrowed, without prominent exudates, sporulation profuse.
Colonies on OA reaching 29–40 mm in diam after 14 d at 25 ºC,
grey-olivaceous, olivaceous-grey to dark smoke-grey, olivaceousblack or iron grey reverse, margin entire edge, narrow, colourless
or white, glabrous, aerial mycelium sparse, mainly in the colony
centre, felty, white to smoke-grey or grey-olivaceous, felty, growth
lat, regular, without exudates, sporulating.
Substrate and distribution: Decaying plant material (on leaves,
stems and other parts of dead herbaceous and woody plants), on
dead fruit bodies of other fungi, occasionally as secondary invader
on lesions caused by other fungi, isolated from human and water,
incl. hypersaline water; widespread, almost cosmopolitan.
Additional specimens examined: Sine loco et dato (L 910.255-723 = L-0115836,
lectotype of Dematium graminum). Sine loco, on dead stems of Brassica sp.
(Brassicaceae), No. 601 [L 910.255-716 = L-0115849, holotype of D. herbarum
var. (β) brassicae]. Sine loco, on leaves of Iris (Iridaceae), Quercus (Fagaceae),
Brassica etc. [L 910.255-736 = L-0115871, holotype of D. vulgare var. (β) foliorum,
isotype L 910.255-718 = L-0115872]. Sine loco et dato [L 910.255-698 = L-0115852,
lectotype of D. vulgare var. (δ) typharum]. Isolated from “Mycosphaerella tulasnei”
(CBS 223.32 = ATCC 11287 = IMI 049635). Czech Republic, Prague, on rotten
leaves of Populus sp. (Salicaceae), 1847, E.D. Hofmann (PRM 657440, neotype
of C. brunneum Corda, designated here). Denmark, Viborg, on dead leaves of
Hordeum vulgare (= H. hexastichum) (Poaceae), 3 Aug. 1903, J. Lind (HBG).
France, Toulouse, on leaves of Phoenix canariensis (= P. tenuis), 1891, G.
Machado, Roumeguère, Fungi Sel. Gall. Exs. 5798 (syntypes of C. phoenicis e.g.,
B, FH). Germany, on dead leaves of Althaea oficinalis (Malvaceae), 8 Sep. 1902,
Zahn, herb. Magnus 1453 (HBG); on petioles of Brassica oleracea (Brassicaceae),
without date, J. Becker (FR); Bavaria, Munich, botanical garden, on fading leaves
of Menispermum canadense (Menispermaceae), Sep. 1894, Allescher (M-0057686,
holotype of C. menispermi); Berlin, Späth’sche Baumschule, on leaves of Idesia sp.
(Salicaceae), Oct. 1895, P. Sydow (B 700006556, holotype of C. idesiae; Sydow,
Mycoth. March. 4498: isotypes, e.g., HBG); Brandenburg, Triglitz, on old fruit bodies
of Hypholoma fasciculata, 2 Oct. 1904, O. Jaap (B). Italy, Emilia Romagna, Parma,
on Equisetum ramosum (Equisetaceae), 1873, G. Passerini (B 700006324, original
material of C. equiseti). Poland, “Tamsel”, on leaves of Yulanea ×soulangeana
(= Magnolia ×soulangeana), Dec. 1904, Vogel (B 700006614, holotype of C.
magnoliae). Romania, isolated from water (CBS 175.82). Russia, Novgorod
Region, Borovichi District, lake Peleno, on dead leaves of Eriophorum vaginatum
(Cyperaceae), 9 Aug. 2007, V.A. Mel'nik (HAL). Slovenia, Sečovlje, isolated from
hypersaline water from salterns (precrystalisation pond), 2004, P. Zalar (EXF-2287
= CPC 12054). Turkey, Ankara, Tekeli, isolated from Triticum aestivum (Poaceae),
isol. S. Tahsin, ident. A.C. Stolk (CBS 299.67). UK, Surrey, Kew, Royal Botanic
the genuS Cladosporium
Gardens, on leaves of Kniphoia uvaria (= K. aloides) (Xanthorrhoeaceae), Aug.
1885, M.C. Cooke (K 121560, holotype of C. kniphoiae). USA, Idaho, Coeur d’
Alene, on leaves, partly becoming dry, of Acer platanoides (Aceraceae), Sep. 1918,
N.W. Scherer, No. 10027 (PAD, type of C. epiphyllum var. acerinum); Montana,
Huntley, on dead leaves of Hordeum sp., 7 Jul. 1928, P.A. Young 238 (BPI 423224);
Seattle, University of Washington Campus, 47.6263530, -122.3331440, isolated
from cleistothecia of Phyllactinia guttata (Erysiphaceae) on leaves of Corylus sp.
(Corylaceae), 16 Sep. 2004, D. Glawe (CPC 11817).
Notes: Cladosporium macrocarpum is closely allied and
morphologically very similar to C. herbarum. The two species
are very common and widespread saprobic species, which
have often been confused previously, depending on the species
concept of C. herbarum s. lat. (McKemy & Morgan-Jones 1991c,
Dugan & Robert 1994, Ho et al. 1999), but based on molecular
and morphological re-examinations Schubert et al. (2007b) have
clearly shown that two distinct species are involved. Cladosporium
macrocarpum is well differentiated from C. herbarum s. str. by the
wider swellings of its nodulose conidiophores, 5–10 μm, broader
and more frequently septate conidia [small terminal conidia
4–11 × (3–)4–6 μm, intercalary conidia 10–17 × (4.5–)5–9 μm,
secondary ramoconidia 14–25(–30) × (5–)6–9(–10) μm] and by
being connected with a distinct teleomorph, described as Davidiella
macrocarpa. The teleomorphic state is characterized by having
smaller, globose pseudothecia, asci with longer stalks, prominent
pseudoparaphyses, and rather inconspicuous luminar ascospore
inclusions.
On natural substrates the conidiophores are usually somewhat
wider than in culture, 4–8(–10) μm wide, and also the conidia can
be somewhat wider, sometimes up to 13(–15) μm.
Type material of C. macrocarpum is not preserved. Therefore,
de Vries (1952) “lectotypiied” C. macrocarpum by a specimen in
Saccardo’s herbarum (Herb. Myc. P.A. Saccardo no. 419, PAD), but
this material, later distributed in Mycotheca Italica no. 1396, has to be
regarded as neotype as already pointed out by David (1997). A single
collection of Saccardo’s Mycotheca Italica no. 1396 from herb. HBG,
which can be considered as isoneotype material, was re-examined
and proved to rather agree with the species concept of C. herbarum
s. str. The conidia were formed in simple, rarely branched chains,
6–26 × (4–)5.5–8(–9) μm, 0–3-septate, almost smooth or minutely
to densely verruculose or verrucose. However, since de Vries’
“lectotypiication” was incorrect according to the code (ICBN, Art. 9.2,
9.17), a neotype was designated in Schubert (2005b).
Cladosporium graminum, described by Persoon (1822), as
well as C. brunneum and C. gracile, introduced by Corda (1837),
are older synonyms of C. macrocarpum and, according to the
code, would have priority. However, since C. macrocarpum is a
well established, currently used name with numerous records in
literature, a proposal to conserve the name against these older
names is in preparation for formal publication in Taxon.
“Purplish” exudates in Cladosporium (examined strain probably
belonging to the C. herbarum complex) have been correlated
with in vivo (in knapweed seed) biological activity against Botrytis
(Ragavendra 2012).
96. Cladosporium maracuja Viégas, Bragantia 6: 367.
1947. Figs 200, 201.
Holotype: Brazil, Prov. St. Pauli, Pindorama, Est. Exp. de
Pindorama, on Passilora sp. (Passiloraceae), 19 Jul. 1935, A.S.
Costa (IACM).
www.studiesinmycology.org
Fig. 200. Cladosporium maracuja (IACM). Conidiophores and conidia in vivo. Scale
bar = 10 µm. K. Bensch del.
Lit.: Schubert (2005b: 99–101).
Ill.: Viégas (1947: 368, ig. 5), Schubert (2005b: 100, ig. 43, pl. 19,
igs A–C).
In vivo: Leaf spots amphigenous, solitary, scattered, subcircular,
1–6 mm wide, paler in the centre, margin reddish brown, surrounded
by a subcircular, yellowish halo. Colonies amphigenous. Mycelium
immersed, intercellular, intraepidermal; hyphae sparingly branched,
4–5.5 µm wide, septate, not constricted at the septa, hyaline to
subhyaline, smooth, walls slightly thickened. Stromata small,
substomatal to intraepidermal, 10–50 µm diam, composed of
swollen hyphal cells, subglobose, 6–10(–14) µm diam, pale brown,
smooth, thick-walled. Conidiophores solitary or in small groups,
arising from swollen hyphal cells or from stromata, erumpent
through the cuticle or emerging through stomata, erect, straight to
somewhat lexuous, sometimes geniculate-sinuous, non-nodulose,
unbranched, rarely branched, 25–85 × 4–7 µm, often slightly
attenuated towards the apex, septate, occasionally somewhat
constricted at the septa, pale brown, smooth, walls only slightly
thickened, often swollen at the base, up to 10 µm wide, protoplasm
of the cells sometimes aggregated at the septa which then appear
to be thickened, similar to distoseptation. Conidiogenous cells
integrated, terminal or intercalary, cylindrical, 10–32 µm long, with
a single to several conidiogenous loci, situated on small lateral
shoulders, protuberant, truncate to slightly convex, 1–2(–2.5) µm
diam, dome and raised rim conspicuous, thickened, darkenedrefractive. Conidia catenate, in branched chains, straight, variable,
obovoid, ellipsoid, fusiform to subcylindrical, 5–25 × (3–)4–6.5 µm,
0–1(–2)-septate, sometimes slightly constricted at the septum,
subhyaline to pale brown, smooth, walls not to only slightly
thickened, apex rounded or with a single to several apical hila,
truncate to slightly convex at the base, hila conspicuous, 1–2 µm
diam, thickened, darkened-refractive; microcyclic conidiogenesis
not observed.
Substrate and distribution: On Passilora sp.; Brazil.
Notes: A stained preparation of the holotype of this species, which
has been examined by F. Freire (Fortaleza, Brazil), showed several
185
BenSch et al.
Fig. 201. Cladosporium maracuja (IACM). A. Conidiophores and conidia. B. Tip of a conidiophore. C. Conidia. Scale bars = 10 (A–C) µm.
fascicles of conidiophores and numerous conidia with conspicuous
cladosporioid conidiogenous loci and hila, well differentiated in a
convex central dome and a raised periclinal rim. Cladosporium
maracuja is the only species in this genus known to attack a
member of Passiloraceae. It is morphologically comparable with
C. praecox, C. orchidearum and C. gentianae, but distinct in having
smooth conidia [faintly to conspicuously verruculose-echinulate
in C. praecox; smooth or almost so to verruculose or irregularly
rough-walled in C. gentianae; verruculose in C. orchidearum],
narrower conidiophores [(4–)5–10 µm wide in C. gentianae], and
usually 0–1-septate conidia (0–3-septate in C. orchidearum).
Cladosporium oxysporum, originally described on dead leaves of
Passilora edulis from Cuba, is a common saprobic species and
easily distinguishable from C. maracuja by having nodulose or
even nodose, much longer conidiophores with conidiogenous loci
conined to swellings.
97. Cladosporium melospermae K. Schub. & U. Braun,
Schlechtendalia 16: 67. 2007. Fig. 202.
Holotype: Argentina, Mendoza, San Rafael, entre Calmu-co y
Barrancas, on leaves of Melosperma andicola (Plantaginaceae),
15 Feb. 1942, Covas, No. 462, ex herb. A. Ruiz Leal, Nr. 10797,
Flora de la Prov. Mendoza (LPS 14053).
Ill.: Braun & Schubert (2007: 68, ig. 4).
In vivo: On leaves and inlorescences (sepals), without conspicuous
leaf spots, but sometimes with pale brownish discolorations,
somewhat faded. Colonies amphigenous, punctiform, scattered to
effuse, brownish, sometimes velvety. Mycelium internal, subcuticular
to intraepidermal; hyphae branched, (3–)5–10 µm wide, septate,
not constricted at the septa, pale brown to pale medium brown or
somewhat olivaceous-brown, sometimes subhyaline, smooth or
almost so, walls more or less thickened, forming aggregations.
Stromata small, substomatal to intraepidermal, 15–60 µm diam,
few layers deep, composed of swollen hyphal cells or hyphal
aggregations, cells 8–15 µm diam, medium brown, smooth, thickwalled. Conidiophores solitary or usually in small loose fascicles,
arising from hyphae aggregations or stromata, emerging through
stomata or erumpent through the cuticle, erect, straight to lexuous,
cylindrical, geniculate-sinuous towards the apex and often coralloid
186
Fig. 202. Cladosporium melospermae (LPS 14053). Fascicle of conidiophores
arising from stromata and conidia in vivo. Scale bar = 10 µm. K. Bensch del.
at the apex, non-nodulose, unbranched, occasionally branched,
20–145 × 4.5–8 µm, 0–5-septate, not constricted at the septa,
medium brown, somewhat paler towards the apex, smooth to
verruculose, walls thickened, up to 1(–1.5) µm thick, often distinctly
two-layered, base often somewhat swollen. Conidiogenous cells
integrated, terminal or intercalary, 10–30 µm long, cylindrical, often
geniculate-sinuous, coralloid, proliferation distinctly sympodial,
with a single or few conidiogenous loci situated on small shoulders
or small lateral projections, protuberant, coronate but dome
the genuS Cladosporium
and rim often lat and not distinctly differentiated, 2–3 µm wide,
thickened and somewhat darkened-refractive. Conidia solitary or
in short unbranched, rarely branched chains, straight, obovoid,
broadly ellipsoid to subcylindrical or somewhat irregular, 9–35 ×
(8.5–)10–15 µm, 0–1(–2)-septate, septum somewhat in the upper
or lower half, sometimes slightly constricted, medium brown,
coarsely verrucose, ornamentation up to 1 µm high, thick-walled,
apiculate, base sometimes attenuated, stalk-like, cells sometimes
with large oil drops, hila more or less protuberant, truncate, dome
and rim lat, dome often not higher than the surrounding rim, 2–3
µm wide, thickened, somewhat darkened-refractive; microcyclic
conidiogenesis occurring.
Substrate and distribution: On Melosperma andicola; Argentina.
Notes: Cladosporium melospermae, occurring on living leaves and
inlorescences of the endemic host species Melosperma andicola,
is morphologically close to C. variabile, but the later species, which
causes distinct leaf spots on spinach, is easily distinguishable by
having longer, (6.5–)10–45(–55) µm, 0–4(–5)-septate conidia.
Cladosporium mimulicola, known from North America on Mimulus
sp., is quite distinct by having shorter and narrower conidiophores
arising from supericial hyphae, smooth and narrower conidia,
(2.5–)3–5(–6.5) µm, and narrower conidiogenous loci and hila. Type
material of C. digitalicola, a species also recorded from a member
of the Scrophulariaceae, was not available for a re-examination.
Therefore, its status remains still uncertain, but conidia were
described to be shorter, 0–4-septate, and conidiophores longer and
narrower, 45–502 × 4.0–7.9 µm (Zhang et al. 1998e).
98. Cladosporium mimulicola U. Braun, Nova Hedwigia
58(1–2): 196. 1994. Fig. 203.
Holotype: USA, California, on Mimulus sp. (Phrymaceae), 18 Jul.
1895, J.J. Davis (NY).
Lit.: Schubert (2005b: 101).
Ill.: Braun (1994: 193, pl. 1, ig. 7), Schubert (2005b: 101, ig. 44).
In vivo: Leaf spots almost absent or diffuse, leaves with brownish
discolorations. Colonies usually hypophyllous, diffuse, brownish.
Mycelium internal and external, supericial; hyphae branched, 2–5
µm wide, septate, brown, smooth, thin-walled. Stromata absent.
Conidiophores solitary, arising from supericial hyphae, lateral
and terminal, straight and subcylindrical to slightly geniculatesinuous, unbranched, 10–80 × 3–5 µm, 0–3-septate, brown, walls
thin to slightly thickened. Conidiogenous cells integrated, terminal
or conidiophores reduced to conidiogenous cell, proliferation
sympodial, conidiogenous loci conspicuous, protuberant, (0.5–)
1–2(–2.5) µm diam, thickened and darkened. Conidia catenate,
often in branched chains, ellipsoid-ovoid, subcylindrical, 10–30 ×
(2.5–)3–5(–6.5) µm, 0–2-septate, brown, smooth, hila coronate,
somewhat protuberant, 0.5–2.5 µm diam, thickened and darkened.
Substrate and distribution: On Mimulus sp.; USA.
Notes: This species, only known from the type collection, is a leafspotting hyphomycete easily distinguishable from other biotrophic
Cladosporium spp. by its solitary conidiophores arising from
supericial hyphae and rather large, smooth conidia.
www.studiesinmycology.org
Fig. 203. Cladosporium mimulicola (NY). Conidiophores arising from supericial
hyphae and conidia in vivo. Scale bar = 10 µm. U. Braun del.
99. Cladosporium minusculum Sacc., Ann. Mycol. 11: 20.
1913. Figs 204, 205.
Holotype: Malta, Ghain el Gbira, on Salix alba (Salicaceae), Oct.
1911, Caruana Gatto (PAD). Isotype: IMI 70294.
Lit.: Ferraris (1914: 886), Saccardo (1931: 798), Braun (2000: 34),
Schubert (2005b: 102–103).
Ill.: Braun (2000: 37, ig. 5), Schubert (2005b: 102, ig. 45, pl. 19,
igs D–F).
In vivo: On living leaves without conspicuous lesions or associated
with distinct leaf spots, together with Alternaria sp., spots
amphigenous, subcircular to irregular, 1–3 mm wide, brownish
to greyish white, with a diffuse brownish margin or marginal
line. Colonies amphigenous, usually epiphyllous, scattered to
subeffuse, dull greyish brown, not very conspicuous. Mycelium
internal, mostly intraepidermal; hyphae sparingly branched, 3–6
µm wide, septate, later often swollen and slightly constricted at
the septa, up to 10 µm wide, subhyaline to pale brown, smooth,
walls somewhat thickened. Stromata absent or developed, small,
usually substomatal, 10–20 mm diam., composed of only few
swollen hyphal cells, somewhat angular, 4–8 µm wide, pale to
medium brown, thick-walled, smooth. Conidiophores solitary or in
small loose fascicles, arising from swollen hyphal cells or stromata,
often emerging through stomata, sometimes erumpent through the
cuticle, erect, straight to often somewhat lexuous, strongly and
frequently geniculate-sinuous, non-nodulose, mostly unbranched,
15–100 × 2–5(–7) µm, pluriseptate, not constricted at the septa,
pale to medium dark brown throughout or tips paler, smooth, with
age somewhat asperulate, walls somewhat thickened, sometimes
187
BenSch et al.
Notes: In the original diagnosis, Saccardo (1913b) described this
species to occur on minute excrements of insects on the lower leaf
surface of living leaves of Salix alba, which could not be observed
neither by Braun (2000) nor during the course of the recent reexamination of the type material. Cladosporium minusculum is
morphologically allied to C. cladosporioides, but differs in having
strongly geniculate-sinuous conidiophores and consistently
verruculose conidia (Braun 2000). Cladosporium lineolatum, which
is also close to C. minusculum, deviates in having an internal
mycelium as well as supericially growing hyphae giving raise to
often branched conidiophores, and usually smooth 0–3-septate
conidia; occasionally ramoconidia are formed.
100. Cladosporium myrtacearum K. Schub., U. Braun &
R.G. Shivas, Australas. Pl. Pathol. 34: 513. 2005. Figs 206,
207.
Fig. 204. Cladosporium minusculum (PAD). Conidiophores and conidia in vivo.
Scale bar = 10 µm. K. Bensch del.
two-layered, protoplasm of the cells somewhat aggregated at the
septa which then appear to be thickened (as in distoseptation).
Conidiogenous cells integrated, terminal and intercalary, 7–35
µm long, proliferation sympodial, with numerous conidiogenous
loci, often aggregated near the apex, protuberant, often situated
on small lateral shoulders, subdenticulate, (0.5–)1–1.5 µm diam,
thickened, refractive to somewhat darkened. Conidia catenate,
in unbranched or branched chains, subglobose, ellipsoid-ovoid,
fusiform to subcylindrical, 3–16 × (2–)3–5(–6) µm, 0–1(–3)-septate,
sometimes slightly constricted at the septa, subhyaline to pale
yellowish, olivaceous or brownish, almost smooth or mostly faintly
to distinctly verruculose, walls somewhat thickened, thicker with
age, protoplasm of the cells mostly with a small, oval to somewhat
irregular, paler cavity in the centre, ends rounded or slightly
attenuated, hila protuberant, truncate to slightly convex, 0.5–1.5
µm diam, thickened, refractive to somewhat darkened; microcyclic
conidiogenesis sporadically occurring.
Substrate and distribution: On Salix alba; Malta.
Holotype: Australia, Northern Territory, Millingimbi, Townsite, on
Corymbia polycarpa (Myrtaceae), 17 Aug. 1999, A.A. Mitchel (BRIP
26527). Isotype: DNAP 26527. Epitype (designated in Bensch et al.
2010): Australia, Northern Territory, Emerald Springs, S13°37’23,
E131°36’40, isol. from Corymbia foelscheana, 22 Sep. 2007, coll.
B.A. Summerell, isol. P.W. Crous (CBS H-20440). Ex-type culture:
CBS 126350 = CPC 14567.
Lit.: Schubert (2005b: 103–105), Bensch et al. (2010: 60–61).
Ill.: Braun et al. (2005: 513, ig. 5), Schubert (2005b: 104, ig. 46, pl.
19, igs G–I), Bensch et al. (2010: 61, ig. 49).
In vivo: Leaf spots amphigenous, subcircular to oval-oblong, 3–12
mm wide, medium to reddish brown, centre paler, occasionally
with a small hole in the centre, margin irregular, inconspicuous
or narrow, somewhat raised, occasionally with a narrow, brown
or purplish halo, inally leaf spots dropping out, leaving shot-hole
symptoms. Colonies amphigenous, mostly in the centre, densely
caespitose, olivaceous-brown. Mycelium immersed, subcuticular
to intraepidermal; hyphae sparingly branched, septate, subhyaline
to pale olivaceous, smooth, thin-walled. Stromata lacking or only
with small hyphal aggregations, composed of swollen hyphal
cells, subglobose to somewhat angular, 5–10(–13) µm diam,
pale olivaceous to medium brown. Conidiophores solitary or
in small, loose to dense fascicles, arising from swollen hyphal
cells, emerging through stomata or erumpent through the cuticle,
Fig. 205. Cladosporium minusculum (PAD). A. Fascicles of conidiophores. B. Frequently geniculate-sinuous conidiophores and conidia. C. Conidia. Scale bars = 10 (A–C) µm.
188
the genuS Cladosporium
Fig. 206. Cladosporium myrtacearum (BRIP 26527). Symptoms, conidiophores and
conidia in vivo. Scale bar = 10 µm. K. Bensch del.
straight to lexuous, unbranched or branched, geniculate-sinuous,
subnodulose, often somewhat attenuated towards the apex
and swollen at the base, 14–96 × 3–7(–9.5) µm, pluriseptate,
often constricted at the septa, pale olivaceous to medium
brown, somewhat paler towards the apex, walls somewhat
thickened, protoplasm of the cells sometimes aggregated at the
septa which then appear to be thickened (as in distoseptation).
Conidiogenous cells integrated, terminal and intercalary,
cylindrical-oblong, 10–43 µm long, proliferation sympodial, with
numerous, scattered conidiogenous loci, not conined to swellings,
somewhat protuberant, 1–2 µm diam, somewhat thickened and
darkened-refractive. Conidia in unbranched or branched chains,
straight, subglobose, ovoid, obovoid, broadly ellipsoid-fusiform,
subcylindrical, 3–22 × 2–7 µm, 0–1(–2)-septate, not to distinctly
constricted at the septa, pale olivaceous to olivaceous, smooth,
wall unthickened to somewhat thickened, apex rounded or with
1–5 hila, base rounded to somewhat attenuated, hila somewhat
protuberant, 1–2 µm diam, somewhat thickened and darkenedrefractive; microcyclic conidiogenesis occasionally occurring.
In vitro: Mycelium branched, branches often only as short lateral
outgrowths, rhizoid, 1−6 μm wide, septate, with swellings and
constrictions, therefore often irregular in outline, subhyaline to
medium olivaceous-brown or dingy brown, smooth to often appearing
rough-walled, minutely verruculose to irregularly rough-walled, walls
unthickened or somewhat thickened, sometimes forming ropes or
hyphae twisted. Conidiophores macronematous, sometimes also
micronematous, solitary or in loose groups, arising terminally and
laterally from ascending, erect and plagiotropous hyphae, erect,
straight to lexuous, cylindrical-oblong, geniculate towards the
apex, sometimes subnodulose towards the tip, but also intercalary,
www.studiesinmycology.org
with unilateral swellings giving conidiophores a gnarled, knotty
appearance, unbranched, occasionally once branched, 9−85(−120)
× (2−)3−4.5(−5) μm, (0−)1−3(−5)-septate, not constricted at septa,
septa sometimes not very conspicuous, pale to medium olivaceousbrown or dingy brown, sometimes paler towards the apex, smooth
or almost so, walls thickened, up to 1 μm wide. Conidiogenous
cells integrated, terminal, cylindrical-oblong, geniculate, once or
sometimes several times, subnodulose with unilateral swellings due
to geniculations, occasionally nodulose, 12−45 μm long, with up to six
loci, crowded towards the apex, situated on small lateral shoulders
but not conined to swellings, loci conspicuous, subdenticulate to
denticulate, 1−1.8 μm diam, thickened and darkened-refractive.
Ramoconidia occasionally formed, 17−30 μm long, about 4 μm wide.
Conidia catenate, in branched chains, branching in all directions, up
to seven conidia in the unbranched terminal part of the chain, smooth
to sometimes verruculose, small terminal and intercalary conidia
obovoid to narrowly ellipsoid, mostly fusiform, 5−10(−12) × 2−4 μm
(av. ± SD: 8.1 ± 1.8 × 2.9 ± 0.6), aseptate, rarely with a single septum,
distinctly attenuated towards apex and base, secondary ramoconidia
fusiform, ellipsoid to subcylindrical, (9−)10−20(−25) × 3−4(−4.5) μm
(av. ± SD: 14 ± 3.4 × 3.5 ± 0.5), 0−1(−2)-septate, not constricted at
septa, septa not very conspicuous, median or somewhat in the upper
half, pale or sometimes medium brown or dingy brown, smooth to
inely verruculose or somewhat irregularly rough-walled, attenuated
towards apex and base, walls unthickened or slightly thickened,
with up to ive distal hila, sometimes also laterally, hila protuberant,
subdenticulate, 0.8−1.8(−2) μm diam, somewhat thickened and
darkened-refractive; microcyclic conidiogenesis occasionally
occurring.
Culture characteristics: Colonies on PDA attaining 40−57 mm diam
after 1 mo, olivaceous-grey, grey-olivaceous towards margins or
iron-grey both surface and reverse, buff due to aerial mycelium,
towards the margins becoming slimy, not sporulating, appearing
zonate, mycelium often aggregated, forming “dread locks”, margin
white, regular, glabrous, growth lat with elevated colony centre,
aerial mycelium formed, often aggregated, without prominent
exudates, sporulating. Colonies on MEA reaching 44−70 mm
diam after 1 mo, iron-grey to smoke-grey due to sporulation,
whitish towards margins, olivaceous-grey reverse, white to buff
towards margins, luffy, hairy, margin white, glabrous, radially
furrowed, aerial mycelium abundantly formed, covering the whole
surface, dense, hairy to luffy, colonies wrinkled, without prominent
exudates, sporulation sparse.
Substrate and distribution: On Myrtaceae (Corymbia, Eucalyptus);
Australia.
Additional specimen examined: Australia, New South Wales, Bimbadeen Lookout,
ca. 10 km of Cessnock, North Coast, isol. from Eucalyptus placita (Myrtaceae), 14
Oct. 2006, coll. B.A. Summerell, isol. P.W. Crous (NSM 734672, CBS 126349 =
CPC 13689).
Notes: Cladosporium myrtacearum seems to be conined to hosts
or substrates of the Myrtaceae and seems to cause lesions,
suggesting a biotrophic fungus. However, the ecology of this
species is not yet clear, i.e. it is uncertain if the observed leaf spots
have been actually caused by C. myrtacearum. It was described
from Australia on Corymbia polycarpa based on a collection in vivo.
Later it was possible to prepare a culture isolated from an additional
collection on the type host, which has been designated as epitype
(Bensch et al. 2010). A second, morphologically well agreeing
189
BenSch et al.
Fig. 207. Cladosporium myrtacearum in vivo (BRIP 26527) and in vitro (CBS 126350). A. Fascicle of conidiophores. B–C. Tips of conidiophores and conidia. D–K. Macronematous
conidiophores and conidial chains in vitro. Scale bars = 10 µm.
strain isolated from Eucalyptus placita from New South Wales
(CPC 13689) clustered with the epitype strain of C. myrtacearum
and formed a highly supported subclade (see Bensch et al. 2010,
ig. 1, part a). Cultures of C. antarcticum resemble those of C.
myrtacearum, but they are distinct by forming dimorphic mycelium,
longer and wider, minutely verruculose or verrucose conidia and
secondary ramoconidia (Schubert et al. 2007b). Cladosporium
jacarandicola (Schubert & Braun 2004), described from New
Zealand on Jacaranda mimosifolia, and C. praecox (Braun 2000)
on Tragopogon orientalis in Europe are two somewhat similar
species, but they differ in having shorter and somewhat narrower,
sparsely septate conidiophores and conidia with different surface
ornamentations (smooth to verruculose in C. jacarandicola and
faintly to conspicuously verruculose-echinulate in C. praecox).
190
101. Cladosporium neriicola S.A. Khan & M.A. Kamal,
Mycopathol. Mycol. Appl. 52(1): 33. 1974. Figs 208, 209.
Holotype: Pakistan, Tando Mohd Khan, Faugi Sugarcane Farm, on
leaves of Nerium oleander (= N. indicum) (Apocynaceae), 11 Oct.
1966, S.A. Khan (IMI 123901).
Lit.: Schubert (2005b: 105–107).
Ill.: Khan & Kamal (1974: 34, ig. 4), Schubert (2005b: 106, ig. 47,
pl. 20, igs A–D).
In vivo: On living leaves, tips and margins of the leaves, faded, turning
pale greyish brown, delimited from green parts of the leaves by a small,
2 mm wide, medium to reddish brown margin, on the lower leaf surface
somewhat darker. Colonies hypophyllous, punctiform, scattered, in
the genuS Cladosporium
Fig. 208. Cladosporium neriicola (IMI 123901). Symptoms, conidiophores and conidia in vivo. Scale bar = 10 µm. K. Bensch del.
Fig. 209. Cladosporium neriicola (IMI 123901). A. Symptoms. B. Geniculate conidiophore showing coronate scar structure. C. Conidiophores. D. Conidia. Scale bars = 5 (B),
10 (C–D) µm.
small tufts, dark brown to blackish. Mycelium internal, immersed,
subcuticular, or external, supericial; hyphae plagiotropic, branched,
2.5–4 µm wide, septate, often constricted at the septa, pale olivaceousbrown, smooth to verruculose, walls slightly thickened. Stromata dense,
15–50 µm diam, only few layers deep, composed of subcircular to
somewhat angular cells, 3–6 µm diam, pale to reddish brown, smooth,
www.studiesinmycology.org
walls slightly thickened. Conidiophores solitary, arising from external,
creeping hyphae, lateral or terminal, or in loose groups arising from
stromata, erect to subdecumbent, straight to slightly lexuous, one to
several times mildly to distinctly geniculate-sinuous, unbranched, rarely
branched, 13–100 × 2.5–4.5(–5) µm, 1–11-septate, pale to medium
brown throughout or somewhat paler towards the apex, smooth or
191
BenSch et al.
faintly to distinctly verruculose, especially at the apex, walls slightly
thickened. Conidiogenous cells integrated, terminal or intercalary,
cylindrical, 6–25 µm long, with a single or several subdenticulate
conidiogenous loci, truncate to slightly convex, 1–2(–2.5) µm diam,
dome and rim often not very conspicuous, somewhat thickened, more
or less darkened-refractive. Conidia catenate, in branched chains,
straight to slightly curved, small conidia subglobose, obovoid, ellipsoid,
3.5–14 × 2.5–4.5(–5) µm, 0–1(–3)-septate, larger conidia cylindricaloblong, 15–30 × 4–5(–6) µm, 1–4(–5)-septate, sometimes constricted
at the septa, mostly at the median septum, subhyaline, pale to medium
brown, smooth to faintly or distinctly verruculose, walls thickened,
apex rounded or attenuated with a single to several apical hila, hila
conspicuous, subdenticulate, truncate to slightly convex, 1–2(–2.5) µm
diam, dome and rim often almost inconspicuous, somewhat thickened,
darkened-refractive; microcyclic conidiogenesis not observed.
Substrate and distribution: On Nerium oleander; Pakistan.
Notes: With its supericially growing, smooth to verruculose secondary
mycelium and its sometimes verruculose conidiophores and conidia,
C. neriicola resembles species of the genus Stenella, but SEM
examinations conclusively showed that C. neriicola is a genuine
member of Cladosporium s. str. (Schubert 2005b, pl. 20, ig. B).
Cladosporium microporum, described on leaves of Nerium
oleander from Italy, proved to be synonymous with C. herbarum. Type
material of C. nerii, recorded on faded and dried leaves of Nerium
oleander from Spain, could not be located in herb. MA and is probably
not preserved. However, the latter species seems to be quite distinct
from C. neriicola, since it was described to have longer and wider,
130 × 7 µm, irregularly pluriseptate conidiophores and wider, oval,
cylindrical to subclavate conidia, up to 30 × 8 µm (Gonzáles-Fragoso
1920). Zhang et al. (2003) reported C. nerii on Nerium indicum from
China, but this record possibly refers to C. neriicola.
102. Cladosporium nigrelloides U. Braun & Mouch., New
Zealand J. Bot. 37(2): 302. 1999. Fig. 210.
Holotype: French Polynesia, Isles Gambier, Mangareva, Rikitaea,
on leaves of Manihot utilissima (Euphorbiaceae), 2 Apr. 1966,
Huguenin (PS 66.684, PC, as “Cercospora henningsii”).
Lit.: Schubert (2005b: 107).
Ill.: Braun et al. (1999: 300, ig. 2), Schubert (2005b: 107, ig. 48).
In vivo: Leaf spots amphigenous, subcircular to irregular, 2–40 mm
wide, sometimes oblong, up to 70 mm in length, ochraceous to
pale brown, later greyish to greyish brown, margin indeinite or with
a narrow somewhat darker marginal line, occasionally somewhat
raised. Colonies amphigenous, punctiform, sometimes dense,
dark greyish brown. Mycelium internal, occasionally with a few
supericial hyphae; hyphae branched, septate, brown, smooth,
thin-walled. Stromata intraepidermal, occasionally substomatal,
15–50 µm diam, brown, composed of swollen hyphal cells, 2.5–8
µm diam, walls somewhat thickened. Conidiophores solitary or in
small to fairly large fascicles, loose to dense, arising from stromata,
erumpent through the cuticle, occasionally emerging through
stomata, erect to decumbent, unbranched or branched, straight,
subcylindrical to slightly geniculate-sinuous, 10–90 × 2.5–6 µm,
olivaceous to medium brown throughout or paler towards the apex,
smooth, walls thin to somewhat thickened. Conidiogenous cells
192
Fig. 210. Cladosporium nigrelloides (PS 66.684, PC). Conidiophores and conidia in
vivo. Scale bar = 10 µm. U. Braun del.
integrated, terminal, conidiogenous loci protuberant, about 1–2 µm
diam, thickened and darkened. Conidia catenate, often in branched
chains, ellipsoid-ovoid, fusiform, subcylindrical, 4–18 × 4–6 µm,
0–3-septate, pale olivaceous to olivaceous-brown, smooth or
almost so, ends obtuse, rounded or attenuated, subacute, hila
protuberant, about 1.5–2 µm diam, thickened and darkened.
Substrate and distribution: On Manihot utilissima; French Polynesia.
Notes: Cladosporium nigrelloides belongs to a group of plantinhabiting, leaf-spotting species within Cladosporium, well
characterised by its relatively large intraepidermal stromata, mostly
fasciculate conidiophores and relatively small conidia which are
more or less smooth.
103. Cladosporium nigrellum Ellis & Everh., Proc. Acad.
Nat. Sci. Philadelphia 1893: 463. 1894. Fig. 211.
Lectotype (designated here): USA, West Virginia, Fayette Co.,
Nuttallburg, on inner bark of railroad ties, 20 Oct. 1893, L.W. Nutall,
Fl. Fayette County, No. 172 (NY). Isolectotypes: Ellis & Everhart, N.
Amer. Fungi. Ser. II, 3086 (e.g., HBG, N, NY, PH, WIS) and Ellis &
Everhart, Fungi Columb. 382 (e.g., NY, PH).
= Cladosporium herbarum f. epixylon Sacc., Mycoth. Ven., Fasc. 3, No. 286.
1875 [syntypes: e.g., BPI 427093, HAL].
= Cladosporium aspericoccum Oudem., in Roumeguère, Fungi Sel. Gall. Exs.,
Cent. 46, No. 4592. 1888, nom. nud. [syntypes: FH].
the genuS Cladosporium
Lit.: Saccardo (1895: 620), Ellis (1976: 329), Morgan-Jones (1977:
5), Zhang et al. (2003: 86–87, 133–134).
Ill.: Ellis (1976: 328, ig. 246), Morgan-Jones (1977: 4, ig. 2), Zhang
et al. (2003: 86: ig. 50; 133, ig. 89).
Exs.: Clements & Clements, Crypt. Format. Colorad. 271; Ellis
& Everhart, N. Amer. Fungi. Ser. II 3086; Ellis & Everhart, Fungi
Columb. 382; Roumeguère, Fungi Sel. Gall. Exs. 4592; Saccardo,
Mycoth. Ital. 591; Saccardo, Mycoth. Ven. 286.
In vivo: Saprobic, occurring on wood. Colonies extended, dense,
caespitose, grey-brown to dark brown or blackish olivaceous,
velvety, often forming expanded layers. Mycelium immersed or
supericial; hyphae branched, 3–6 µm wide, septate, sometimes
slightly constricted at septa, pale brown to pale yellowish-brown,
sometimes almost hyaline, smooth, walls slightly thickened, often
with small swellings and constrictions, forming stromatic hyphal
aggregations, cells subcircular to somewhat angular-irregular,
5–10(–14) µm wide, medium to dark brown, walls thickened.
Conidiophores macronematous, solitary or in small loose groups,
arising from swollen hyphal cells or stromatic hyphal aggregations,
erect, straight to somewhat lexuous, cylindrical, sometimes
slightly geniculate-sinuous and with small intercalar swellings, up
to 8 µm wide, unbranched, rarely branched, 14–290 × 4–7(–9)
µm, sometimes slightly attenuated towards apex and swollen
at the base, pluriseptate, not constricted at septa, medium to
dark brown, paler towards the apex, pale brown at the apex,
smooth, occasionally minutely verruculose towards the apex,
walls thickened, sometimes distinctly two-layered, protoplasm
sometimes aggregated at septa and walls. Conidiogenous cells
integrated, terminal or intercalary, cylindrical, sometimes with small
intercalar swellings, proliferation sympodial, polyblastic, with up to
four conidiogenous loci, sometimes occurring at small swellings
but not conined to them, truncate to slightly convex, 1–2(–2.5)
µm diam, somewhat thickened and darkened-refractive. Conidia
catenate, in unbranched or branched chains, straight or slightly
curved, subglobose, obovoid, limoniform, ellipsoid to subcylindrical,
3.5–22(–31) × 3–8(–10) µm, 0–3(–4)-septate, sometimes slightly
to distinctly constricted at septa, subhyaline, pale brown to medium
brown, smooth, sometimes verruculose, walls unthickened or
slightly thickened, protoplasm aggregated at walls and septa, apex
and base rounded or slightly attenuated, with up to three apical
scars, slightly to distinctly protuberant, base truncate to slightly
convex, 1–2(–2.5) µm diam, not distinctly differentiated in dome
and surrounding rim in light microscopy, thickened and somewhat
darkened-refractive; microcyclic conidiogenesis occasionally
occurring.
Substrates and distribution: On wood and herbaceous stems; Asia
(China), Europe (France, Italy, Russia), North America (USA).
Additional specimens examined: Italy, Bologna, botanical garden, on dead stems
of Ricinus communis (Euphorbiaceae), Feb. 1899, Saccardo, Mycoth. Ital. 591
(SIEN, as C. stromatum); Selva (Treviso), on decorticated branches of Robinia
pseudoacacia (Fabaceae), Sep. 1874, Saccardo, Mycoth. Ven. 286 (e.g., BPI
427093, HAL, syntypes of C. herbarum f. epixylon). Latvia, Vidzeme, Vestiena,
on dead wood of Picea abies (Pinaceae), 20 Jul. 1933, K. Starcs (M-0057726).
Netherlands, La Haye, on dead stems of Sambucus nigra (Adoxaceae), May
1888, C. Destrée, Roumeguère, Fungi Sel. Gall. Exs. 4592 (FH, syntype of C.
aspericoccum). Russia, Leningradskaya Oblast’, Vyborgskij Rajon, settlement
Roshchino, on dead wood of Betula pendula (Betulaceae), 21 Sep. 2006, D.A.
Shabunin (HAL 2402 F); Novgorodskaya Oblast', Okulovskij Rajon, in the vicinity of
village Zarechnaya, on dead wood of Betula pendula, 20 May 2001, D.A. Shabunin
(HAL 2403 F), contains Cladosporium herbarum and traces of C. nigrellum. USA,
Colorado, Bottomless Pit, on dead cones of Pinus lexilis (Pinaceae), 13 Jul. 1906,
Clements & Clements, Crypt. Format. Colorad. 271 (BPI 426469, as C. entoxylinum).
www.studiesinmycology.org
Fig. 211. Cladosporium nigrellum (NY). Conidiophores and conidia in vivo. Scale
bar = 10 µm. K. Bensch del.
Notes: The colonies, conidiophores and conidia of this woodinhabiting species resemble those of the saprobic C. delicatulum
but the conidia in C. nigrellum are somewhat wider [3–8(–10) µm
in C. nigrellum versus 3–6(–7) µm in C. delicatulum]. Besides
this the biology of C. nigrellum seems to be different from C.
delicatulum in mainly occurring on wood. Therefore, we prefer to
treat it as a distinct species. Additional collections and phylogenetic
studies are necessary to clarify the status of this species as well
as of the morphologically very similar species C. sarmentorum
also occurring on wood. Since the conidiophores of the latter
species are frequently branched in the upper portion with short
lateral branchlets or long branches, it is tentatively maintained as
a species separate from C. nigrellum until additional collections,
cultures and phylogenetic data are available.
Cladosporium xylophilum, a species also occurring on
wood, deviates from C. nigrellum in having shorter and narrower
conidiophores, 0–1(–3)-septate, narrower conidia, true ramoconidia
and in forming numerous subglobose or ovoid small terminal conidia
which are often irregularly rough-walled (Bensch et al. 2010).
The description and illustration of “C. nigrellum” in Ho et al. (1999:
137 and 138, ig. 38) was based on a strain isolated from Fagus
sylvatica leaf litter in the Netherlands and does not agree with true
C. nigrellum but was re-identiied as Fusicladium fagi (Crous et al.
2007d).
193
BenSch et al.
Substrate and distribution: On Zinnia elegans; Italy.
Notes: Cladosporium oblongum resembles C. herbarum and C.
macrocarpum, but the conidiophores of C. oblongum are less
nodulose, the conidiogenous loci are not conined to swellings and,
above all, the conidia are characteristically coarsely verrucose, with
rather large, distant verrucae. On account of conidia often formed
singly or only in simple chains and the very coarse sculpture
of the conidial surface, this species is close to various former
Heterosporium species.
105. Cladosporium obtectum Rabenh. ex Cooke, Grevillea
17(83): 66. 1889. Figs 213, 214.
≡ Cladosporium obtectum Rabenh., in Marcucci, Unio Itin. Crypt., No. 36.
1866, nom. nud.
Lectotype (designated here): Italy, Sardinia, Alghero, on fading
leaves of Artemisia maritima (Asteraceae), Marcucci, Unio Itin.
Crypt. 36 (HBG). Isolectotypes: Marcucci, Unio Itin. Crypt. 36;
Rabenhorst, Fungi Eur. Exs. 2783 (e.g., B, HAL, HBG, M).
Lit.: Saccardo (1892: 602, 1895: 619), Ferraris (1912: 348), Ellis
(1976: 342), Schubert (2005b 108–109).
Ill.: Ellis (1976: 341, ig. 259 C), Schubert (2005b: 108, ig. 49, pl.
19, igs J–L).
Exs.: Marcucci, Unio Itin. Crypt. 36; Rabenhorst, Fungi Eur. Exs.
2783.
Fig. 212. Cladosporium oblongum (BPI 427289). Stromata, fascicle of conidiophores
and conidia in vivo. Scale bar = 10 µm. U. Braun del.
104. Cladosporium oblongum Bres., Stud. Trent. 7: 74.
1926. Fig. 212.
Holotype: Italy, Trento, on dead stems of Zinnia elegans
(Asteraceae), 1923, G. Bresadola (BPI 427289).
In vivo: Saprobic on dead stems, colonies punctiform or pustulate,
up to 5 mm diam, dark olivaceous-brown to brown. Mycelium
internal. Stromata well-developed, at irst small to medium-sized,
punctiform, 20–100 μm diam, composed of swollen hyphal cells,
3–10 μm diam, thick-walled, stromata later enlarged or conluent,
up to 500 μm diam, pustulate, subcircular in outline to somewhat
irregular or oblong, at irst immersed, later erumpent, brown.
Conidiophores arising from stromata, erumpent, in large, loose
to dense fascicles, erect, straight, subcylindrical to moderately
geniculate-sinuous, subnodulose, swellings usually connected
with geniculation, often unilateral, i.e. not as nodulose as in C.
herbarum, unbranched, 100–250 × 3–7 μm, pluriseptate throughout,
olivaceous-brown, wall up to 1 μm wide, almost smooth to distinctly
asperulate. Conidiogenous cells integrated, terminal, occasionally
intercalary, 10–30 μm long, conidiogenous loci 1.5–2 μm diam,
slightly protuberant. Conidia solitary or catenate, usually in simple
chains, broadly ellipsoid-ovoid, subcylindrical, rarely subglobose,
8–25 × 5–10 μm, rather pale, very pale yellowish to greenish,
pale olivaceous or olivaceous-brown, wall up to 1 μm thick, very
coarsely verrucose-rugose, verrucae up to 1 μm wide, distant from
each other (up to 2.5 μm), ends usually broadly rounded, hila 1.5–2
μm wide and 1 μm high; microcyclic conidiogenesis not observed.
194
In vivo: On leaves, without distinct leaf spots or discolorations.
Colonies hypophyllous, sometimes also epiphyllous, punctiform
to subeffuse, pale olivaceous-brown, thin, mostly not very
conspicuous, between leaf hairs. Mycelium external, supericial;
hyphae branched, 3–11 μm wide, with swellings and constrictions,
pluriseptate, closely septate, cells narrow, 3–10 μm long, pale
to medium olivaceous-brown, smooth, walls thickened, forming
loose to dense, small to extended stromatic hyphal aggregations.
Conidiophores solitary or in loose to somewhat denser groups,
but not fasciculate, sometimes forming large, expanded,
sporodochium-like layers, arising from external hyphae or
stromatic hyphal aggregations, more or less erect and lexuous,
non-nodulose, sometimes slightly geniculate-sinuous, unbranched
or branched, 5–60 μm long, but usually much shorter, 3–5(–7)
μm wide, 0–2(–3)-septate, sometimes slightly constricted at the
Fig. 213. Cladosporium obtectum (HBG). Conidiophores and conidia in vivo. Scale
bar = 10 µm. K. Bensch del.
the genuS Cladosporium
Fig. 214. Cladosporium obtectum (HBG). A. External pluriseptate mycelium. B. Conidiophores. C. Conidium. Scale bars = 10 µm.
septa, pale to medium olivaceous-brown, smooth, walls more or
less thickened. Conidiogenous cells terminal or conidiophores
often reduced to conidiogenous cells, later becoming also
intercalary, 5–25 μm long, sometimes slightly geniculate-sinuous
towards the apex, proliferation sympodial, with a single or several
protuberant conidiogenous loci, often situated on small shoulders,
subdenticulate, short cylindrical, obconically truncate, 1.5–2.5 μm
diam, thickened and darkened-refractive. Conidia catenate, usually
in short unbranched chains, occasionally in branched chains,
straight or somewhat curved, broadly ellipsoid to cylindrical, 8–25(–
34) × (3–)5–8 μm, 0–5-septate, often 3-septate, sometimes slightly
constricted at the septa, occasionally with a single longitudinal
septum, septa becoming somewhat sinuous with age, pale to
medium olivaceous-brown, smooth or sometimes verruculose,
walls more or less thickened, slightly attenuated towards apex
and base, hila protuberant, obconically truncate, 1.5–2.5 μm diam,
thickened and darkened-refractive; microcyclic conidiogenesis
occasionally occurring.
Substrate and distribution: On Artemisia maritima; Italy.
Notes: Unio Itin. Crypt. (1866) is a selection of species made by
Marcucci and determinated by Rabenhorst. Since a description
was not given, C. obtectum was not validly published, but in
Cooke’s “Omitted Diagnoses” (1889) a brief Latin diagnosis of
this species was provided. Ellis (1976) described longer conidia,
up to 34 μm, and somewhat wider conidiophores, which could not
be observed. Zhang et al. (2003) reported this species from China
on Artemisia hedinii and Solanum melongena. These records are
very probably not identical with C. obtectum since the authors
described and illustrated much longer conidiophores, 51–102 μm,
and 0–2-septate, shorter and narrower conidia.
Several other Cladosporium species occur on hosts belonging
to the Asteraceae. Cladosporium agoseridis on Agoseris glauca in
North America and C. praecox, known from the Czech Republic
on Tragopogon orientalis, are easily distinguishable by internal
mycelium, usually fasciculate conidiophores and 0–1(–3)-septate
conidia with different surface ornamentations (loosely to densely
verruculose or verrucose in C. agoseridis; faintly to distinctly
verrucose-echinulate in C. praecox). Cladosporium inopinum,
known from South America on Gynoxys hallii, differs in forming
small stromata, often fasciculate, longer conidiophores, up to 120
μm, and narrower conidia, 2.5–6 μm wide.
www.studiesinmycology.org
Fig. 215. Cladosporium oncobae (HAL 1832 F). Symptoms, conidiophores and
conidia in vivo. Scale bar = 10 µm. K. Bensch del.
106. Cladosporium oncobae K. Schub. & C.F. Hill, Fungal
Diversity 22: 18. 2006. Figs 215, 216.
Holotype: New Zealand, Auckland, Princes Street, Auckland
University Campus, on Oncoba spinosa (Salicaceae), 19 Sep.
2004, C. F. Hill, No. 1076 (HAL 1832 F).
Lit.: Schubert (2005b: 109–111).
Ill.: Schubert (2005b: 110, ig. 50, pl. 21, igs A–G), Braun et al.
(2006: 19–20, ig. 2, pl. 2).
195
BenSch et al.
sometimes enteroblastically proliferating, often somewhat swollen at
the base. Conidiogenous cells integrated, terminal and intercalary,
8–36 μm long, proliferation sympodial, somewhat geniculatesinuous, with few to numerous conidiogenous loci, often crowded
and situated on small lateral shoulders, protuberant, 0.5–2(–2.5)
μm diam, thickened, more or less darkened-refractive. Conidia
in branched chains, numerous, variable in shape, subglobose,
obovoid, limoniform, narrowly to broadly ellipsoid to subcylindrical
or somewhat irregular, 3–20(–25) × 2.5–6(–7) μm, 0–3-septate,
occasionally constricted at the septa, pale olivaceous, smooth, very
rarely somewhat rough-walled, walls thickened, often rounded at the
ends, usually with a single or few apical protuberant hila, 0.5–2(–2.5)
μm diam, thickened, more or less darkened-refractive; microcyclic
conidiogenesis occurring.
Substrate and distribution: On Oncoba spinosa; New Zealand.
Fig. 216. Cladosporium oncobae (HAL 1832 F). A. Symptoms. B, C. Tips of
conidiophores with numerous, conspicuous, somewhat crowded conidiogenous
loci and conidia. D. Conidiophore and conidia. E. Numerous conidia and base of a
conidiophore with percurrent, enteroblastic proliferation and thickened, two-layered
walls. F. Conidial chains. G. Conidial chain. Scale bars = 5 (D, G), 10 (B–C, E–F)
µm.
In vivo: On living leaves, causing necrotic leaf margins, leaf spots
amphigenous, small to extended, irregular in shape, infections mostly
starting at leaf margins, later enlarging and covering large areas of
the leaf surface, pale to dark brown, sometimes somewhat zonate, at
irst without deinite border, later with a distinct, small to wide, irregular,
dark brown to purple-brown margin, often turning fragile at the leaf
margins. Colonies amphigenous, scattered, loosely caespitose,
pale olivaceous-grey to dark olivaceous-brown. Mycelium internal,
subcuticular, rarely external; hyphae emerging through stomata
and growing supericially, creeping, loosely branched, 2–6 μm wide,
septate, sometimes slightly constricted at the septa, often with small
swellings, pale olivaceous, smooth, walls slightly thickened, forming
a loose network, at the base of the conidiophores often somewhat
swollen and darker, pale to medium olivaceous-brown. Stromata
mostly absent to rarely well-developed, substomatal, 15–40 μm diam,
forming dense stromatic aggregations composed of swollen hyphal
cells, subglobose, 6–13 μm diam, pale to medium or dark olivaceousbrown, thick-walled. Conidiophores mostly solitary, rarely in pairs or in
small groups, arising from swollen hyphal cells or from internal, rarely
supericial hyphae, usually emerging through stomata, erect, straight
to lexuous, often somewhat geniculate-sinuous, subnodulose, with
small lateral shoulders or one-sided swellings, sometimes with
somewhat head-like, swollen tips, unbranched or rarely branched,
15–162 × (2.5–)3–6(–7) μm, pluriseptate, pale olivaceous to medium
or dark olivaceous-brown, often somewhat paler at the apex, smooth,
walls thickened, often distinctly two-layered, (0.5–)1–2 μm thick,
196
Notes: Cladosporium idesiae, the only species of the genus
Cladosporium hitherto described on a host belonging to
the Flacourtiaceae, has to be reduced to synonymy with C.
macrocarpum.
Cladosporium oncobae is morphologically comparable with C.
cladosporioides, C. myrtacearum and C. uredinicola. However, C.
cladosporioides differs from the new species in having somewhat
longer and narrower, 0–1-septate conidia, usually terminal
conidiogenous cells only with a single or few conidiogenous
loci and conidiophores with only somewhat thickened and
usually one-layered walls; in C. myrtacearum the conidiophores
are often arranged in loose to somewhat dense fascicles,
crowded conidiogenous loci are lacking, and the conidia are
0–1(–2)-septate; and in the hyperparasitic C. uredinicola the walls
of the conidiophores are only slightly thickened, not two-layered
and the conidia are longer, 3–39 μm, 0–2(–3)-septate, without
any constrictions (Heuchert et al. 2005). Cladosporium alneum
is also morphologically closely allied to C. oncobae, but differs
in its occurrence on an unrelated host (on Alnus spp.), distinct
lesions and conidiophores with thinner walls [0.5–1 μm wide in
C. alneum, versus (0.5–)1–2 μm wide in C. oncobae]. Based on
these differences, and since leaf-spotting Cladosporium species
are generally conined to related hosts of a single plant family, C.
oncobae is considered a separate species.
Two strains isolated from type material and stored in the
CPC collection (CPC 11663 and CPC 11664) at the CBS were
included in the phylogenetic studies within the C. cladosporioides
complex. CPC 11664 represents one of the C. cladosporioides s.
lat. lineages and CPC 11663 is conspeciic with C. perangustum
(see Bensch et al. 2010). Cladosporium perangustum differs from
C. oncobae in having narrower conidiophores, conidiogenous cells
and conidia and probably co-occured with the latter species on the
same leaf spots. Since C. oncobae is morphologically close to C.
cladosporioides it is possible that CPC 11664 is ex-type culture of
C. oncobae. However, additional isolates from Oncoba leaves are
necessary to clarify the status of the isolate and the species.
107. Cladosporium orchidearum Cooke & Massee,
Grevillea 16(79): 80. 1888. Figs 217, 218.
Holotype: Great Britain, Surrey, Kew, Kew Gardens, H. Low’s
nursery, Borough of Richmond, on leaves of Oncidium crispum
(Orchidaceae) (NY 72454).
the genuS Cladosporium
Fig. 217. Cladosporium orchidearum (NY 72454). Conidiophores emerging through
stomata or erumpent through the cuticle and conidia in vivo. Scale bar = 10 µm.
K. Bensch del.
Lit.: Saccardo (1892: 605), Ellis (1976: 339), Schubert & Braun
(2004: 305), Schubert (2005b: 111–113).
Ill.: Ellis (1976: 339, ig. 257 B), Schubert & Braun (2004: 306, ig.
4), Schubert (2005b: 112, ig. 51, pl. 20, igs E–G).
In vivo: On living leaves, distinct leaf spots lacking. Colonies
hypophyllous, punctiform, in small tufts, scattered to dense, dark
olivaceous-brown. Mycelium immersed, subcuticular; hyphae
sparingly branched, 2–4.5 μm wide, septate, not to slightly
constricted at the septa, sometimes with small swellings, up to
7 μm wide, subhyaline, pale olivaceous to pale brown, smooth,
walls not or only slightly thickened. Stromata 25–65 μm diam,
composed of subcircular to somewhat angular or oblong cells,
5–11 μm wide, pale to medium brown, walls slightly thickened.
Conidiophores solitary or in small fascicles, arising from stromata,
mostly emerging through stomata, or erumpent through the
cuticle, erect, straight to slightly lexuous, sometimes somewhat
geniculate-sinuous, unbranched, occasionally branched, 20–75
× (3–)4–6 μm, 0–3-septate, pale to medium olivaceous-brown,
smooth, walls not or only very slightly thickened, not or somewhat
swollen at the base, up to 7 μm wide. Conidiophores reduced to
conidiogenous cells or conidiogenous cells integrated, terminal,
20–45 μm long, proliferation sympodial, with one to several
conidiogenous loci, often crowded at the apex, loci protuberant,
subdenticulate to denticulate, truncate to slightly convex, 1.5–2.5
μm diam, slightly thickened, darkened-refractive. Conidia catenate,
usually in branched chains, ellipsoid, ovoid, subcylindrical, 5–20 ×
4–7 μm, 0–3-septate, sometimes slightly constricted at the septa,
pale olivaceous, almost smooth to usually verruculose, walls only
slightly thickened, somewhat attenuated towards apex and base,
with up to three scars at the apex, hila truncate to slightly convex,
1–2(–2.5) μm diam, thickened, darkened-refractive; microcyclic
conidiogenesis not observed.
Fig. 218. Cladosporium orchidearum (NY 72454). A. Symptoms. B, C. Conidiophores and conidia with conspicuous, darkened-refractive conidiogenous loci. Scale bars = 10
(B–C) µm.
www.studiesinmycology.org
197
BenSch et al.
Substrate and distribution: On Oncidium crispum; Great Britain.
Notes: Cladosporium orchidearum is only known from the type
material; several collections from BPI identiied as “C. orchidearum”
proved to be misidentiied and had to be referred to other taxa or they
had to be described as new species. Cladosporium orchidearum
belongs into a complex of foliicolous Cladosporium species with
consistently short conidiophores, but differs in having narrower
conidiophores [(3–)4–10(–13) wide in C. agoseridis; 3–8 μm in C.
lupiniphilum] and smaller, above all narrower conidia, respectively
[(6.5–)12–26(–31) × 4–8 μm in C. praecox; (10–)12–40(–50) × (5–)
6–13(–15) μm in C. agoseridis; 6–28 × 4–8 μm in C. lupiniphilum].
Cladosporium orchidis, described on Dactylorhiza majalis ssp.
praetermissa from Great Britain, was excluded and re-allocated to
Fusicladium and C. cattleyae, known from Belgium on dead leaves
of Cattleya mossia, proved to be a synonym of Dendryphiella
vinosa (Schubert & Braun 2004).
108. Cladosporium orchidiphilum K. Schub. & U. Braun,
Sydowia 56(2): 306. 2004. Figs 219, 220.
Holotype: Australia, Canterbury, on leaves of Cypripedium sp.
(Orchidaceae), 19 Aug. 1914 (VPRI 2488, as “C. orchidearum”).
Lit.: Schubert (2005b: 113–115).
Ill.: Schubert & Braun (2004: 307, ig. 5), Schubert (2005b: 114, ig.
52, pl. 22, igs A–C).
In vivo: On living leaves, causing leaf spots, irregular in shape, on
the upper leaf surface with a greyish centre, surrounded by a dark
reddish brown margin, on the lower leaf surface more or less zonate,
with a pale greyish brown centre, surrounded by dark red-brown and
pale reddish brown segments, partly surrounded by a narrow dark
red-brown margin and a pale to dark reddish brown halo. Colonies
hypophyllous, punctiform, in small tufts, scattered, blackish brown.
Mycelium internal; hyphae sparingly branched, 2–4 μm wide,
septate, often slightly constricted at the septa, cells sometimes
slightly swollen, pale olivaceous to pale olivaceous-brown, smooth,
walls slightly thickened. Stromata dense, small to large, substomatal,
subcuticular to intraepidermal, 35–75(–100) μm diam, composed
of subglobose to somewhat angular or irregular, thick-walled cells,
up to 13 μm wide, medium to dark brown, smooth. Conidiophores
mostly densely fasciculate, arising from stromata, emerging through
Fig. 219. Cladosporium orchidiphilum (VPRI 2488). Fascicle of conidiophores
emerging through stomata and conidia in vivo. Scale bar = 10 µm. K. Bensch del.
stomata or erumpent through the cuticle, more or less erect,
straight to lexuous, often somewhat geniculate-sinuous, nodulose,
but swellings not connected with any conidiogenous loci, mostly
unbranched, rarely branched, 50–175 × (2.5–)3–6 μm, pluriseptate,
medium brown, paler towards the apex, smooth, thick-walled,
sometimes two-layered, 0.5–1 μm wide, somewhat attenuated
towards the apex, often enteroblastically proliferating, visible as
discontinuity in pigmentation and thickness of the wall, protoplasm
sometimes aggregated at the septa, so that the walls and septa
appear to be thickened, similar to distoseptation. Conidiogenous cells
integrated, terminal or intercalary, 12–35 μm long, with numerous,
often crowded conidiogenous loci, sometimes situated on small
lateral shoulders, more or less protuberant, subdenticulate, 0.5–1.5
μm diam, slightly thickened, darkened-refractive. Conidia catenate,
in unbranched or branched chains, straight to curved, subglobose,
ovoid, ellipsoid to somewhat irregular in shape, 3–15(–19) × 2–5(–7)
Fig. 220. Cladosporium orchidiphilum (VPRI 2488). A. Fascicle of conidiophores emerging through stomata. B, C. Conidiophores and conidia. Scale bars = 10 (B–C), 20 (A) µm.
198
the genuS Cladosporium
μm, 0–3(–4)-septate, sometimes constricted at the septa, usually
very pale to pale brown, rarely somewhat darker, pale medium
brown, smooth to minutely verruculose, thick-walled, sometimes with
distinct, pale, irregular lumen in the centre of the cell, surrounded by
the somewhat darker protoplasm, apex rounded or attenuated, often
with one or several apical hila, hila protuberant, truncate to slightly
convex, 0.5–1.5 μm diam, slightly thickened, somewhat darkenedrefractive; microcyclic conidiogenesis often occurring.
Substrate and distribution: On Cypripedium; Australia.
Notes: Cladosporium orchidearum is a similar species, but easily
distinguishable by having shorter, 0–3-septate conidiophores,
without swellings and enteroblastic proliferations, and wider
conidiogenous loci and hila, (1–)1.5–2.5 μm diam; microcyclic
conidiogenesis does not occur. The identity of the host plant is not
quite clear. Cypripedium spp. are not native in Australia, and they
are not commercially grown. The cultivation of these orchids is very
dificult, and they are generally only found in specialist hobby orchid
collections. Confusion with commercially grown Paphiopedilium
or Phragmipedium spp. is possible (according to a previous
information from C.F. Hill †).
109. Cladosporium oreodaphnes Allesch. ex K. Schub.,
Schlechtendalia 14: 73. 2006. Figs 221, 222.
Fig. 221. Cladosporium oreodaphnes (M-0057756). Symptoms, fascicle of
conidiophores and conidia in vivo. Scale bar = 10 μm. K. Bensch del.
≡ Cladosporium oreodaphnes Allesch., in herb.
Holotype: Germany, Berlin, botanical garden, on a leaf of
Oreodaphne foetens [= Ocotea foetens] (Lauraceae), Apr. 1894,
P. Hennings (M-0057756).
Lit.: Schubert (2005b: 115–117).
Ill.: Schubert (2005b: 116, ig. 53, pl. 23, igs A–G), Schubert et al.
(2006: 74, ig. 9, pl. 1, ig. G).
In vivo: Leaf spots amphigenous, effuse, covering large areas of
the leaf surface, faded, turning pale brownish-grey or somewhat
ochraceous, membranous, caused by the detaching cuticle, margin
or marginal line mostly small, irregular, dark brown or olivaceousbrown, sometimes with a small olivaceous-greyish halo. Colonies
amphigenous, scattered, punctiform, dense, in tufts, greyish brown
to somewhat blackish, appearing darker on the upper leaf surface,
somewhat loccose or sometimes villose. Mycelium internal,
subcuticular to intraepidermal; hyphae branched, 2–5 μm wide,
septate, sometimes with swellings and constrictions, very pale,
subhyaline or almost hyaline to pale olivaceous, smooth or almost
so, walls only slightly thickened. Stromata small to moderately
large, 25–65 μm wide, sometimes wider, up to 110 μm wide,
several layers deep, composed of swollen hyphal cells, subglobose
to somewhat angular, polygonal, 5–11 μm wide, medium to dark
olivaceous-brown or brown, smooth, thick-walled. Conidiophores
in small to moderately large loose fascicles, arising from stromata,
erumpent through the cuticle, more or less erect, straight to more
or less lexuous, cylindrical-oblong to iliform, sometimes mildly
geniculate-sinuous, often subnodulose to nodulose, intercalar
swellings up to 7 μm diam, unbranched to often branched, usually
once branched, sometimes several times branched, 40–230 × 3.5–7
μm, pluriseptate, sometimes slightly constricted at the septa, pale to
medium olivaceous-brown, smooth or almost so to faintly asperulate
or irregularly rough-walled with age, walls somewhat thickened, up
to 1 μm thick, sometimes even two-layered, occasionally swollen
www.studiesinmycology.org
at the base, up to 8 μm wide, protoplasm of the cells sometimes
aggregated at the septa appearing to be thickened, similar to
distoseptation. Conidiogenous cells integrated, terminal and
intercalary, 10–21 μm long, proliferation sympodial, occasionally
mildly geniculate-sinuous, often subnodulose or nodulose, usually
with numerous, crowded conidiogenous loci at the swellings,
but loci not conined to them, protuberant, subdenticulate, 1–2.5
μm diam, thickened and darkened-refractive. Conidia catenate,
in branched chains, more or less straight, almost globose to
subglobose, obovoid, limoniform, somewhat fusiform, ellipsoid to
subcylindrical, 2.5–19(–24) × 2–7(–8) μm, 0–1(–3)-septate, pale to
medium olivaceous-brown, almost smooth to minutely asperulate or
irregularly rough-walled, walls more or less thickened, sometimes
even two-layered, up to 1 μm wide, with distinct lumen in the centre
of the cells, surrounded by the somewhat darker protoplasm, apex
and base somewhat rounded or attenuated, hila protuberant,
truncate to obconically truncate, short cylindrical, 0.5–2(–2.5) μm
diam, thickened, darkened-refractive; microcyclic conidiogenesis
occurring.
Substrate and distribution: On Oreodaphne foetens; Germany.
Notes: Several Cladosporium species have been described from
hosts belonging to the Lauraceae, but almost all of them have
to be excluded from the genus. Cladosporium cinnamomeum
(≡ Scolecotrichum cinnamomeum) was assigned to Stenella (Braun
2002) and now belongs to the genus Zasmidium (see excluded
species). Cladosporium congestum proved to be conspeciic with
Spiropes scopiformis (Ellis 1968). Cladosporium ferrugineum,
known from Cuba on Nectandra coriacea, was placed in the new
genus Penidiella (Crous et al. 2007b). Cladosporium machili (nom.
inval.), described from Taiwan on Machilus (= Persea) thunbergii,
and C. supericiale on Cinnamomum ovalifolium from India have
to be excluded from Cladosporium s. str. since the conidiogenous
199
BenSch et al.
Fig. 223. Cladosporium ornithogali (M). Conidiophores and conidia in vivo. Scale bar
= 10 µm. K. Bensch del.
Lectotype: Germany, on leaves of Ornithogalum umbellatum
(Asparagaceae), spring, Klotzsch, Herb.Viv. Mycol. 69 (M, selected
by David 1997). Isolectotypes: Klotzsch, Herb.Viv. Mycol. 69 (e.g.
HAL, K).
= Heterosporium ornithogali var. minus Bäumler, Ann. K.K. Naturhist. Hofmus.
12(2): 81. 1897, as “f. minus“ [syntypes: Krypt. Exs. 219 (e.g., K)].
= Didymellina ornithogali J.E. Jacques, Contr. Inst. Bot. Montréal 39: 35. 1941.
≡ Davidiella ornithogali (J.E. Jacques) Crous & U. Braun, Mycol. Progr.
2(1): 10. 2003.
Fig. 222. Cladosporium oreodaphnes (M-0057756). A. Symptoms. B. Overview. C.
Conidiophores with numerous, conspicuous, somewhat crowded conidiogenous
loci. D. Branched conidiophore with several conidiogenous loci. E. Conidia showing
cell structure, with paler cavity in the centre of the cells. F. Conidiophore with several
coronate scars. G. Conidia. Scale bars = 5 (F–G), 10 (C–E), 50 (B) µm.
loci and hila are non-cladosporioid, but their taxonomic afinities
are not yet clear. Cladosporium lauri was described to form leaf
spots on leaves of Laurus nobilis which were damaged by insects.
Type material of the latter species could not be traced, but Raybaud
(1923) stated that the conidia are similar to those of the genus
Torula with the central cells swollen and voluminous.
Among leaf-spotting Cladosporium species on hosts of other
plant families, Cladosporium oreodaphnes is morphologically
comparable with C. apicale, C. dracaenatum and C. fusicladiiformis;
however, the latter species is easily distinguishable by its dimorphic
conidiophores and somewhat shorter and narrower conidia, which
are usually smooth or almost so. Cladosporium apicale possesses
much longer and distinctly attenuated conidiophores with thicker
walls, 0.75–3 μm wide, and in C. dracaenatum the conidiophores
and conidia are narrower, intercalar swellings with crowded
conidiogenous loci are lacking and, above all, the conidia become
longer, wider, more frequently septate and more thick-walled with
age.
110. Cladosporium ornithogali (Klotzsch ex Cooke) G.A.
de Vries, Contr. Knowl. Genus Cladosporium: 49. 1952. Fig.
223.
Basionym: Heterosporium ornithogali Klotzsch ex Cooke, Grevillea
5(35): 123. 1877.
≡ Heterosporium ornithogali Klotzsch, Herb. Viv. Mycol., Cent. I, No. 69.
1832, nom. inval.
200
Lit.: Ellis (1976: 338), David (1995b; 1997: 52), IMI Descriptions of
Fungi and Bacteria, No. 123, Sheet 1228.
Ill.: Ellis (1976: 337, ig. 256 B), David (1995b: 1, ig.; 1997: 53–54,
igs 14 A–F, 15).
Exs.: Beck & Zahlbruckner, Krypt. Exs. 219; Ellis & Everhart,
Fungi Columb. 1366; Ellis & Everhart, N. Amer. Fungi Ser. II.
2187; Klotzsch, Herb. Viv. Mycol. 69; Saccardo, Mycoth. Ital. 1588;
Săvulescu, Herb. Mycol. Roman. 646; Scheuer, Dupla Graec. Fung.
6; Tranzschel & Serebrianikow, Mycoth. Ross. 248; Vestergren,
Micromyc. Rar. Sel. Praec. Scand. 1445; Vize, Micro-Fung. Brit. 70.
In vivo: Leaf spots amphigenous, elongated ellipsoid patches,
5–20 × 2–5 mm, the long axis parallel to that of the leaves, pale to
dark brown, becoming conluent, somtimes covering the entire leaf
surface. Colonies effuse, caespitose, loose to dense, pale to dark
brown, velvety. Mycelium internal, subcuticular to intraepidermal;
hyphae branched, 2–7 μm wide, septate, not constricted at the
septa, hyaline, subhyaline to very pale olivaceous, smooth,
walls unthickened or almost so, forming swollen hyphal cells.
Stromata absent (only formed in culture, black, prosenchymatous).
Conidiophores solitary, in loose groups or caespitose, arising
from hyphae or swollen hyphal cells, emerging through stomata
or erumpent through the cuticle, erect, more or less straight,
geniculate-sinuous, often somewhat twisted, subnodulose to
nodulose, swellings up to 15 μm wide, unbranched or apically
once branched, (12–)25–120(–200) × (7–)8–12 μm, septate,
younger conidiophores often only with a single or few basal septa,
not or only slightly constricted at septa, pale olivaceous-brown to
red-brown, paler at the apices, subhyaline, smooth or minutely
roughened, walls thin, base often somewhat wider or swollen,
up to 15 μm. Conidiogenous cells integrated, usually terminal,
also intercalary, geniculate, once or several times, proliferation
sympodial with conidiogenous loci situated on lateral shoulders or
the genuS Cladosporium
swellings, distinctly protuberant, (2–)3(–4) μm wide, ca. 1 μm high,
the periclinal rim generally lower than the central dome, thickened
and somewhat darkened-refractive. Conidia solitary or in short
unbranched chains, young conidia oblong-ovate, 0–1-septate, later
oblong-cylindrical, straight to slightly curved, (12–)20–60(–75) ×
(7–)9–15(–16) μm, (0–)2–6(–7)-septate, usually not constricted at
the septa, becoming sinuous with age, very pale to pale brown,
verrucose to coarsely verrucose, walls almost thin-walled, with
age more thickened, up to 1 μm, apex rounded, but somewhat
attenuated towards the base, base truncate, hila protuberant,
ca. 3 μm diam, thickened and darkened-refractive; microcyclic
conidiogenesis not observed.
Substrates and distribution: On Ornithogalum spp. and Gagea
spp. (Liliaceae); Africa (Kenya, South Africa), Europe (Austria,
Czech Republic, France, Germany, Hungary, Italy, Montenegro,
Netherlands, Poland, Romania, Russia, Serbia, Slovakia, UK), North
America (USA) – Gagaea lutea (Germany), G. pratensis (Czech
Republic, Germany), G. pusilla (Austria, Russia), Ornithogalum
arabicum (Kenya), O. narbonense (France, Montenegro, Serbia),
O. nutans (Hungary, N. Ireland, Slovakia), O. umbellatum (Austria,
France, Germany, Italy, Poland, Romania; USA, IL, PA, WA),
Ornithogalum sp. (Netherlands, South Africa, UK).
Additional specimens examined: Czech Republic, Bohemia, Tábor, on Gagea
pratensis, 25 Mar. 1905, F. Bubák, Vestergren, Micromyc. Rar. Sel. Praec. Scand.
1445 (M). Germany, Sachsen-Anhalt, Altmark, Altkreis Osterburg, Gollensdorf,
Friedhof, MTB 3035/1, on Gagea pratensis, 22 Apr. 2003, H. Jage, Jage Herb.
No. 167/03 (HAL); Börde, Kreis Köthen, Gröbzig, Friedhof, on Gagea pratensis,
23 Mar. 2003, H. Jage, Jage Herb. No. 61/03 (HAL); Hügelland, Kreis Hettstedt,
Ober-Wiederstedten, Kirch(Fried)hof, MTB 4335/1.3, on Gagea pratensis, 12 Apr.
2003, H. Jage, Jage Herb. No. 100/03 (HAL); on Ornithogalum umbellatum, 12 Apr.
2003, H. Jage, Jage Herb. No. 99/03 (HAL). Hungary, Posonia, in a garden, on
Ornithogalum nutans, May, J.A. Bäumler, Beck & Zahlbruckner, Krypt. Exs. 219
(M, syntype of Heterosporium ornithogali f. minus). Romania, Muntenia, distr.
Ilfov, Jilava, on Ornithogalum umbellatum, 8 May 1933, T. Săvulescu & C. Sandu,
Săvulescu, Herb. Mycol. Roman. 646 (M). Russia, Prov. Tambow, Zna, on Gagea
pusilla, 22 Apr. 1910, Schirajewsky, Tranzschel & Serebrianikow, Mycoth. Ross. 248
(M). USA, Pennsylvania, Philadelphia, on Ornithogalum umbellatum, Dr. G.A. Rex,
Ellis & Everhart, N. Amer. Fungi. Ser. II. 2187 (M).
Fig. 224. Cladosporium ossifragi (M). Fascicle of conidiophores and conidia in vivo.
Scale bar = 10 µm. K. Bensch del.
Notes: Ranojević (1910) reported this species on leaves of
Ornithogalum narbonense, Muscari comosum and M. neglectum
from Montenegro and Serbia. Material on Muscari could not be
traced and examined.
111. Cladosporium ossifragi (Rostr.) U. Braun & K. Schub.,
Stud. Mycol. 58: 133. 2007. Figs 224–227.
Basionym: Napicladium ossifragi Rostr., Bot. Fǽröes 1: 316. 1901.
Fig. 225. Cladosporium ossifragi (CBS 842.91). Conidiophores and conidia in vitro.
Scale bar = 10 µm. K. Bensch del.
Neotype (selected in Schubert et al. 2007b): Denmark, Undallslund,
on Narthecium ossifragum (Nartheciaceae), 13 Sep. 1885, E.
Rostrup (CP). Epitype: Norway, Bjerkreim County, isolated from
Narthecium ossifragum, M. di Menna (CBS H-19860). Ex-epitype
culture: CBS 842.91 = ATCC 200946.
Ill.: David (1995a: 1, ig.; 1997: 88–89, igs 21, 22 A–B), Ho et al.
(1999: 133, igs 28–29), Schubert et al. (2007b: 133–134, igs.
26–28).
Exs.: Jaap, Fungi Sel. Exs. 49; Petrak, Mycoth. Gen. 146; Sydow,
Mycoth. Germ. 1047.
≡ Heterosporium ossifragi (Rostr.) Lind, Dan. fung.: 531. 1913.
= Heterosporium magnusianum Jaap, Schriften Naturwiss. Vereins SchleswigHolstein 12: 346. 1902 [lectotype: HBG].
≡ Cladosporium magnusianum (Jaap) M.B. Ellis, More Dematiaceous
Hyphomycetes: 337. 1976.
Lit.: Ellis & Ellis (1985: 390), David (1995a, 1997: 85), IMI
Descriptions of Fungi and Bacteria, No. 123, Sheet 1227 (1995),
Ho et al. (1999: 132).
www.studiesinmycology.org
In vivo: On living or fading leaves without causing distinct leaf spots,
but sometimes with discolorations. Colonies amphigenous, scattered
to effuse, caespitose, dense, pale to medium or even dark olivaceousbrown, with dense fructiication velvety. Mycelium internal, subcuticular
to intraepidermal; hyphae sparingly branched, septate, subhyaline to
pale olivaceous, smooth. Stromata small to usually extended, dense,
compact, 25–100 μm wide or even larger, later conluent, substomatal
201
BenSch et al.
Fig. 226. Cladosporium ossifragi (CBS 842.91). A. Macronematous conidiophore. B. Micronematous conidiophore. C–D. Conidia. E. Conidia and microcyclic conidiogenesis.
Scale bars = 10 µm.
Fig. 227. Cladosporium ossifragi (CBS 842.91). A. Survey on different secondary ramoconidia and conidia. B. Details of conidia and hila. Note the very pronounced ornamentation
and the absence of ornamentation near the site of spore formation. C. Detail of the end of a secondary ramoconidium with pronounced hila. D. Formation of a new conidium.
Note the broad scar behind it (> 1 µm). E. Formation of a new conidium from a smooth-walled stalk. F. Hila on a secondary ramoconidium. This micrograph is from the sample
before coating with gold-palladium and shows similar features as the sample after sputter coating. Scale bars = 2 (B–D, F), 5 (E), 10 (A) µm.
202
the genuS Cladosporium
to intraepidermal, composed of slightly swollen hyphal cells, 4–7 μm
wide, pale to medium olivaceous or olivaceous-brown, smooth, walls
slightly thickened. Conidiophores in small to usually large, loose to
somewhat denser fascicles, emerging through stomata or erumpent
through the cuticle, arising from stromata, erect, straight to lexuous,
non-nodulose but often slightly geniculate, unbranched, up to 250 μm
long, (3–)5–7 μm wide, sometimes attenuated towards the apex, 2 μm
wide, septate, not constricted at septa, pale to medium olivaceous,
golden brown with age, paler towards the apex, subhyaline, walls
almost smooth to usually minutely verruculose, walls unthickened
or almost so, with age becoming thickened, two distinct wall layers
visible, 1–1.5 μm thick. Conidiogenous cells terminal or intercalary,
cylindrical, often slightly geniculate, proliferation sympodial with several
small conidiogenous loci often situated on small lateral shoulders,
protuberant, 1–2 μm diam, thickened, refractive or slightly darkened.
Conidia solitary or catenate, in unbranched chains, straight, cylindrical
to subfusiform, ellipsoid, rounded at the ends, (10–)16–32(–37)
× (5–)7–10(–12) μm, (0–)1–5(–7)-septate, mostly 2–4-septate,
occasionally constricted at septa, pale to medium brown, closely
verruculose to echinulate, more or less thick-walled, hila protuberant,
1–2 μm diam, thickened, somewhat refractive or darkened; microcyclic
conidiogenesis not observed.
In vitro: Mycelium abundantly formed, twisted, often somewhat
aggregated, forming ropes, branched, 1–5 μm wide, septate,
often irregularly swollen and constricted, hyaline or subhyaline to
pale brown, smooth, walls unthickened or only slightly thickened.
Conidiophores macronematous and micronematous, arising from
plagiotropous hyphae, terminally or laterally, erect to subdecumbent,
more or less straight to lexuous, cylindrical, sometimes geniculate,
subnodulose with loci often situated on small lateral shoulders,
unbranched, sometimes branched, often very long, up to 350 μm
long, 3–4.5(–5) μm wide, pluriseptate, shorter ones aseptate, not
constricted at septa, pale to pale medium brown, paler towards
apices, sometimes subhyaline, smooth to minutely verruculose,
especially towards apices, walls somewhat thickened, up to 0.5
μm, sometimes appearing two-layered. Conidiogenous cells
integrated, terminal as well as intercalary, cylindrical, sometimes
geniculate, subnodulose, 5–31 μm long, proliferation sympodial,
with few loci (1–3) per cell, loci usually conined to small lateral
shoulders, protuberant, conspicuous, short cylindrical, 1–2 μm
wide, up to 1 μm high, somewhat thickened, darkened-refractive.
Conidia catenate, in short, unbranched or branched chains,
straight, small terminal and intercalary conidia subglobose, obovoid
to ellipsoid, 4–15 × 3–5 μm [av. ± SD, 9.3 (± 3.7) × 4.0 (± 0.7)
μm], 0–1-septate, not constricted at the septa, pale brown, hila
0.8–1 μm diam, secondary ramoconidia cylindrical, sometimes
ellipsoid or subfusiform, 16–36(–40) × (4–)5–8 μm [av. ± SD, 26.6
(± 7.4) × 6.0 (± 1.2) μm], (0–)1–3(–4)-septate, not constricted at
the septa, septa sometimes slightly sinuous, pale brown to pale
medium brown, densely verruculose, verrucose to echinulate
(densely muricate under SEM), walls unthickened to somewhat
thickened, rounded or somewhat attenuated at apex and base,
hila protuberant, conspicuous, sometimes situated on short, small
prolongations, 1–2.5 μm diam, somewhat thickened and darkenedrefractive; microcyclic conidiogenesis occasionally occurring.
Culture characteristics: Colonies on PDA reaching 53 mm diam after
14 d at 25 ºC, greenish olivaceous, grey-olivaceous to olivaceousgrey or iron-grey, appearing somewhat zonate, dull green to
olivaceous-black reverse, margin colourless, regular, entire edge,
aerial mycelium abundantly formed, covering at irst the colony
www.studiesinmycology.org
centre later most of the surface, dense, high, growth lat with elevated
colony centre, somewhat folded. Colonies on MEA reaching 54 mm
diam after 14 d at 25 ºC, pale olivaceous-grey to olivaceous-grey
in the centre, iron-grey reverse, velvety, margin colourless to white,
entire edge, radially furrowed, aerial mycelium abundantly formed,
luffy to felty, growth lat with somewhat raised, folded colony centre.
Colonies on OA attaining 52 mm diam after 14 d at 25 ºC, olivaceousgrey to iron-grey, iron-grey to greenish black reverse, margin white,
entire edge, aerial mycelium diffuse, loose, growth lat, prominent
exudates absent, sporulation profuse on all media.
Substrate and distribution: Causing leaf spots on Narthecium
ossifragum; Europe (Austria, Denmark, Germany, Great Britain,
Ireland, Norway).
Additional specimens examined: Denmark, Tǿnder, Rǿmǿ, Twismark (formerly
belonging to Germany, on the label: Germany, Schleswig-Holstein), on Narthecium
ossifragum, 19 Aug. 1911, H. Sydow, Sydow, Mycoth. Germ. 1047 (M, PDD 51942).
Germany, Hamburg, Eppendorfer Moor, on N. ossifragum, 12 Sep. 1897, O. Jaap
[HBG, as “Heterosporium ossifragi”, lectotype of C. magnusianum (desingated
by Schubert et al. 2007b)]; 4 Sep. 1903, O. Jaap, Jaap, Fungi Sel. Exs. 49 (M);
Hamburg, Wernerwald near Cuxhaven, on N. ossifragum, Aug. 1927, A. Ludwig,
Petrak, Mycoth. Gen. 146 (M). Norway, Møre og Romsdal County, on N. ossifragum,
M. di Menna (CBS 843.91).
Notes: Due to rather large conidia with coarse surface ornamentation,
this species was included in a revision of the Cladosporium herbarum
complex (Schubert et al. 2007b). Type material of Napicladium
ossifragi is not preserved in Rostrup’s herbarium (on Narthecium
ossifragum, Faeroe Islands, Viderö, Viderejde and Österö, Svinaa,
sine dato, leg. Ostenfeld & Harz). However, other authentic
collections seen and examined by Rostrup are deposited at CP.
Lind (1913) re-examined these samples, synonymised N. ossifragi
with H. magnusianum and correctly introduced the combination H.
ossifragi. Nevertheless, the correct oldest name for this fungus has
been ignored by most authors. David (1997), who clearly stated that
N. ossifragi is the earliest name for this species, preferred to use
the name C. magnusianum because the typiication of Rostrup’s
name was still uncertain. Despite the lacking type material, there
is no doubt about the correct identity of N. ossifragi since authentic
material of this species, examined by and deposited in Rostrup’s
herbarium (CP), is preserved. Therefore, there is no reason to
reject the oldest valid name for this species. The original collection
of C. magnusianum cited by Jaap (1902) (on leaves of Narthecium
ossifragum, Denmark, Tǿnder, Rǿmǿ, peatbog by Twismark, Jul.–
Aug. 1901, Jaap), but not designated as type, is not preserved
(David 1997). It is neither deposited at B, HBG nor S. However, in
the protologue Jaap (1902) also referred to material of this species
found near Hamburg, which was, hence, syntype material available
for a lectotypiication proposed by Schubert et al. (2007b).
112. Cladosporium oxysporum Berk. & M.A. Curtis, J. Linn.
Soc., Bot. 10: 362. 1869. Figs 228, 229.
Holotype: Cuba, on dead leaves of Passilora sp. (Passiloraceae),
C. Wright, Fungi cubensis Wrightiani, No. 489 (K 121562).
= Cladosporium subtile Rabenh., Fungi Eur. Exs., Ed. Nov., Ser. 2, Cent. 24,
No. 2364. 1876, nom. nud. [syntypes: e.g. B, HAL, HBG].
= ?Cladosporium atriellum Cooke, Grevillea 6(40): 139. 1878 [syntypes:
Ravenel, F. Amer. Exs. 296, e.g., K, NY, PH 01020433].
= Cladosporium artocarpi Gonz. Frag. & Cif., Bol. Soc. Esp. Hist. Nat. 25: 366.
1925 and Publ. Estac. Agron. Haina, Ser. B, Bot. 1: 14. 1925 [holotype: MA
06393].
203
BenSch et al.
macronematous, arising from immersed hyphae or swollen hyphal
cells, erect, cylindrical-iliform, straight to slightly sinuous, but
barely geniculate, unbranched, nodulose to distinctly nodose, (50–)
70–350(–400) × (2.5–)3–6(–7) μm, at the very base occasionally
somewhat enlarged, up to 10 μm wide, nodulose swellings 4–9 μm
diam, pale to mid brown throughout or somewhat paler towards
the apex, smooth or almost so, wall slightly thickened, 0.5–1(–1.5)
μm. Conidiogenous cells integrated, terminal and intercalary,
10–60 μm long, conidiogenous loci distinctly coronate, conined
to nodulose swellings, (1–)2–4(–6), slightly protuberant, 1–2.5 μm
diam, thickened and darkened. Ramoconidia lacking or developed,
but not very abundant, up to 25(–30) × 5–6 μm, 1–2(–3)-septate.
Conidia catenate, in branched chains, small terminal conidia and
intercalary conidia subglobose, obovoid, ellipsoid-ovoid, broadly
fusiform, limoniform, 3–15 × 2.5–5 μm, 0(–1)-septate, secondary
ramoconidia abundant, ellipsoid-ovoid, subcylindrical, fusiform,
8–20(–25) × (3–)4–6 μm, 0–1(–2)-septate, with 2–4 terminal hila,
pale olivaceous, olivaceous-brown to brown, smooth or almost so,
thin-walled, 0.5–0.8 μm, hila slightly protuberant, 0.8–2 μm diam;
microcyclic conidiogenesis not observed.
Fig. 228. Cladosporium oxysporum (CBS 125991). Macro- and micronematous
conidiophores, conidia and microcyclic conidiogenesis with conidia forming
secondary conidiophores in vitro. Scale bar = 10 µm. K. Bensch del.
= Cladosporium sorghi S.R. Chowdhury, Sydowia 23(6): 50. “1969” 1970
[holotype: IMI 125190b].
Lit.: Saccardo (1886: 363), Ellis (1971: 312), McKemy & MorganJones (1991b), David (1997: 81), Bagyanarayana & Braun (1999:
13), Ho et al. (1999: 137), de Hoog et al. (2000: 589), Zhang et al.
(2003: 138–139), Schubert & Braun (2004: 308–309), Heuchert et
al. (2005: 48), Schubert (2005b: 159–160), Bensch et al. (2010:
61–63).
Ill.: Ellis (1971: 313, ig. 216 A), McKemy & Morgan-Jones (1991b:
399, pl. 1; 401, ig. 1; 403, ig. 2), David (1997: 62, ig. 17 A–E),
Ho et al. (1999: 138, ig. 39), de Hoog et al. (2000: 589–590, igs),
Zhang et al. (2003: 139, ig. 93), Bensch et al. (2010: 62–63, igs
50–51).
In vivo: Colonies effuse, rarely punctiform, dingy olivaceous to
greyish brown, thinly hairy to velvety, loose to moderately dense.
Mycelium immersed, occasionally supericial, hyphae branched,
2–5 μm wide, septate, subhyaline, pale olivaceous to brownish,
smooth, thin-walled, with age sometimes becoming variously
inlated, swollen hyhal cells sometimes forming loose to moderately
dense aggregations or sometimes strands. Stromata lacking, but
sometimes with small to moderately large, lat, often loose, stromatic
hyphal aggregation, composed of swollen hyphal cells, 2.5–10(–
15) μm diam, yellowish to medium brown, smooth, wall soemwhat
thickened, 0.5–1.5 μm wide. Conidiophores solitary to caespitose,
forming loose aggregations, distinct fascicles lacking, erumpent,
204
In vitro: Mycelium internal and supericial, hyphae loosely branched,
1–4 μm wide, septate, not constricted at septa, but sometimes irregular
due to swellings and constrictions, subhyaline to pale olivaceous,
darker towards the base of the conidiophores, medium olivaceousbrown, smooth, walls unthickened or slightly thick-walled, occasionally
forming ropes. Conidiophores macronematous, sometimes also
micronematous, solitary, arising terminally and laterally from hyphae,
erect, straight to slightly lexuous, nodose with nodes being quite
apart of each other, with a single node at the apex or just below and
few additional nodes on a lower level, 1–7 nodes per conidiophore,
swellings 3–6 μm wide, unbranched, rarely branched, conidiophores
long, 40–720 μm or even longer, 2–4 μm wide, at the base up to
5 μm wide, slightly attenuated towards the apex, pluriseptate, not
constricted at septa, pale to medium olivaceous-brown, sometimes
dark brown, often paler at the apex, smooth, walls somewhat thickwalled, about 0.5(–1) μm wide; micronematous conidiophores paler,
narrower and shorter, also with nodules or only subnodulose or
geniculate, often attenuated towards the apex, 30–115 × 1.5–2 μm,
nodes 3–4 μm wide. Conidiogenous cells integrated, terminal and
intercalary, with a single node, conidiogenous loci conined to these
swellings, with 1–4 loci per node, sometimes subnodulose or once
geniculate, 14–46 μm long, loci conspicuous, subdenticulate, 0.8–1.5
μm diam, somewhat thickened and darkened-refractive. Ramoconidia
rarely occurring. Conidia catenate, in branched chains, up to ive
conidia in the terminal unbranched part of the chain, branching in all
directions, small terminal conidia globose, subglobose to obovoid,
3–5 × 2–3 μm (av. ± SD: 4.2 ± 0.6 × 2.5 ± 0.4), aseptate, apex
rounded, intercalary conidia ovoid, limoniform to ellipsoid, (4–)5–
11 × 2.5–3.5(–4) μm (av. ± SD: 7.2 ± 1.9 × 3.0 ± 0.4), aseptate,
rarely 1-septate, not constricted at septa, with 2–5(–6) distal hila,
attenuated towards apex and base, secondary ramoconidia ellipsoid
to subcylindrical, 7–21(–24) × (2.5–)3–4 μm (av. ± SD: 15.0 ± 5.1 ×
3.3 ± 0.4), 0–1-septate, not constricted at septa, pale olivaceous to
pale olivaceous-brown, smooth, walls unthickened or almost so, with
2–4(–5) distal hila, subdenticulate, 0.5–1.5(–2) μm diam, thickened
and darkened-refractive; sometimes microcyclic conidiogenesis
occurring with conidia forming secondary conidiophores.
Culture characteristics: Colonies on PDA attaining 68–78 mm diam
after 14 d, smoke-grey to pale olivaceous-grey, reverse leadengrey, grey-olivaceous at margins both surface and reverse, felty-
the genuS Cladosporium
Fig. 229. Cladosporium oxysporum (CBS 125991). A–B. Tips of macronematous conidiophores, in A typically nodulose, and conidial chains. C–H. Intercalary conidiogenous
cells and conidia. Scale bar = 10 µm.
loccose, margins broad, regular, whitish, glabrous, aerial mycelium
abundant, covering most parts of the colony surface, feltyloccose or luffy, growth low convex, without prominent exudates,
sporulating. Colonies on MEA attaining 58–70 mm diam after 14 d,
whitish to smoke-grey or pale olivaceous-grey at margins, reverse
olivaceous-grey to black, woolly-loccose, margins colourless
to whitish, glabrous, regular, radially furrowed, aerial mycelium
abundant, covering most of the colony surface, woolly-loccose,
whitish to smoke-grey, growth low convex, without prominent
exudates, sporulation sparse. Colonies on OA reaching 53–62 mm
diam after 14 d, whitish to pale olivaceous-grey or smoke-grey,
reverse leaden-grey, pale olivaceous-grey at margins, woollyloccose, margins colourless, narrow, glabrous, regular, aerial
mycelium abundant, white to smoke-grey, densely woolly-loccose,
covering most of the colony surface, growth lat to low convex,
sometimes several prominent exudates, sporulation sparse.
Substrates and distribution: Isolates from air, soil, on dead parts
of leaves and stems of herbaceous and woody plants and other
organic matter; common and widespread, especially in the tropics
and subtropics.
Additional specimens examined: Sine loco et dato, on Ficus hispida (Moraceae)
(M-0057754). Australia, Toowoomba, isolated from Cucumis melo fruits
(Cucurbitaceae), 31 Oct. 1977, P.E. Meyers T8069 (IMI 223440). China, terracotta
army site, isol. from soil, 2000, coll. S. Gravesen, isol. B. Andersen (CBS H-20441,
CBS 125991 = CPC 14371, BA 1738, reference strain of C. oxysporum). Cuba,
Boyamo, on pods of Adenanthera pavonina (Fabaceae), 13 Sep. 1967, R. Urtiaga
(IMI 130161). India, Ludhiana, isolated from Arachis hypogaea (Fabaceae), 16
Apr. 1973, J.S. Chohan 199 (IMI 1555); Madhya Pradesh, Raipur, Coll. of Science,
on inlorescences of Sorghum bicolor (= S. vulgare) (Poaceae), Dec. 1966, S.R.
Chowdhury (IMI 125190b, holotype of C. sorghi). Mexico, intercepted at Laredo,
Texas, USA by Cary, on Oncidium sp. (Orchidaceae), 13 Apr. 1954, det. A.H. Lewis
(BPI 427293, as C. orchidearum). New Caledonia, Plateau de la Chute de la
www.studiesinmycology.org
Riviere des Lacs, isolated from Dacrydium araucarioides (Podocarpaceae), 15 Sep.
1066, B. Huguenin L25 (IMI 123453). USA, Florida, Winter Haven, on Citrus leaves,
21 Jun. 1967, E. Fisher (IMI 128147); Texas, on leaves of Ulmus sp. (Ulmaceae),
1894 (NY, as C. brunneum Corda); Ellis (NY). Venezuela, isol. from indoor air before
renovation in lab, 2007, coll. K. Lyhne, isol. B. Andersen (BA 1707 = CBS 126351
= CPC 14308).
Notes: Cladosporium oxysporum is well-characterised by a
combination of nodulose conidiophores, as in C. herbarum and
allied species, and relatively small, smooth conidia. It is a rather
common and widespread saprobic hyphomycete, especially in
tropical and subtropical areas, inhabiting a wide range of ecological
niches, including dead parts of leaves and stems of herbaceous
and woody plants, also as secondary invader on necrotic leaf
lesions caused by other fungi, and living host plants on which it has
been recorded to induce leaf spots (Fisher 1967, Hammouda 1992,
Lamboy & Dillard 1997). Type material of C. oxysporum has been
re-examined, described and illustrated by McKemy & MorganJones (1991b) and David (1997), conirming the interpretation
of the species by Ellis (1971) to be accurate. Type material is in
poor condition and almost exhausted, but a few typical conidia and
nodulose conidiophores could be observed (Schubert 2005b).
The number of examined strains of C. oxysporum was rather
limited, and cultures from Cuba or other Carribean areas were not
at hand. Therefore, an epitypiication is postponed until appropriate
collections will be available, but CBS strain 125991 from China,
which agrees very well with the species concept of C. oxysporum,
can serve as reference strain.
An unusual phenomenon of the two strains included in Bensch
et al. (2010) on PDA is that the conidiophores do not possess the
typical swellings, but are unbranched or branched, once or several
times geniculate-sinuous, secondary ramoconidia are longer, up to
37 μm long and true ramoconidia are formed.
205
BenSch et al.
CBS strain 125.80, which was used as reference strain for C.
oxysporum in de Hoog et al. (2000), Wirsel et al. (2002) and Zalar
et al. (2007) and illustrated by de Hoog et al. (2000) with nodose
conidiophores, is not conspeciic with this species. Morphologically
it belongs to the C. cladosporioides s. lat. complex, and clusters
quite apart from C. oxysporum (see Bensch et al. 2010, ig. 1, part
a vs. c) which suggests that it probably got contaminated.
113. Cladosporium pannosum Cooke, Grevillea 12(61): 24.
1883, sub “Chaetophoma musae Cooke”. Fig. 230.
Holotype: USA, South Carolina, on Musa sp. (Musaceae), H.W.
Ravenel, No. 3056 (K 121564).
Lit.: Saccardo (1884: 201), Heuchert et al. (2005: 48–50).
Ill.: Heuchert et al. (2005: 49, ig. 19).
In vivo: Colonies medium brown, effuse, conluent, velvety, also
on the necrotic leaf tissue of the host plant. Mycelium internal and
external; hyphae 2–5 μm wide, septate, often constricted at the
septa, hyphal cells occasionally swollen, up to 10 μm wide, medium
to dark brown, forming dense hyphal aggregations or stromata,
which are composed of swollen subglobose hyphal cells, 5–10
μm diam, medium brown, smooth. Conidiophores solitary, in loose
groups or fasciculate, arising from swollen hyphal cells or stromata,
erect, straight to slightly curved, geniculate-sinuous, unbranched
or only rarely branched, 7–126 × (2–)3–5(–6) μm, up to 6 μm wide
at the very base, 0–4-septate, without or with constrictions at the
septa, pale to medium brown, somewhat paler towards the apex,
smooth to faintly rough-walled, walls slightly thickened, unthickened
near the tips, usually < 1 μm. Conidiogenous cells integrated,
terminal and intercalary, 5–45 μm long, polyblastic, proliferation
sympodial, with several more or less protuberant, thickened and
somewhat darkened-refractive conidiogenous loci, 1–2 μm diam.
Conidia usually in branched chains, straight, subglobose, obovoid,
ellipsoid, subcylindrical, limoniform, 5–18(–25) × 2–6(–7) μm,
0–3-septate, non-constricted at the septa, pale olivaceous-brown
to medium brown, smooth or almost so to verruculose, apex
rounded to somewhat attenuated, with up to ive hila, base truncate
to convex, hila more or less protuberant, thickened, refractive, 1–2
μm diam; microcyclic conidiogenesis occurring.
Substrate and distribution: Saprobic, on dead leaves and stems;
Europe (Italy), North America (USA).
Additional specimen examined: Italy, Siena, Botanical Garden, on dead stems of
Echeandia echeandoides (= E. eleutheranda) (Asparagaceae), Nov. 1898, without
collector (SIENA).
Notes: The name Cladosporium pannosum was introduced by
Cooke in connection with the ascomycete Chaetophoma musae
and meant to be its possible conidial form (anamorph). However,
this association is undoubtedly wrong since C. pannosum is a true
member of Cladosporium s. str. The ecology of C. pannosum is
unclear. An association with other fungi has not been observed.
It is very probably a saprobic fungus, which was found together
with Chaetophoma musae on the same leaves by accident.
Cladosporium pannosum is C. cladosporioides-like, but differs
in having intercalary conidiogenous cells, often with numerous
conidiogenous loci. Furthermore, the conidiophores tend to be
geniculate. Cooke (1883) published the following original diagnosis:
206
Fig. 230. Cladosporium pannosum (K 121564). Conidiophores, solitary or in loose
groups arising from swollen hyphal cells or stromata, conidiogenous cells and
conidia in vivo. Scale bar = 10 µm. B. Heuchert del.
“Effusa, atro-fusca, pannosa. Hyphis intertextis, cladosporoides
(Cladosporium pannosum, Cooke). Peritheciis globoso-depressis,
membrenaceis, fuscis (.08 mm. diam). Sporis ellipticis, hyalinis
(.004 × .002 mm)”.
A collection on rotten leaves of Prunus lyonii (USA, California,
St. Catalina, Golf Links, 27 Dec. 1920, L.W. Nuttall, F 1166312) is
close to C. pannosum, but the conidia are consistently verruculose.
A sample from Brazil (State of Ceará, São Benedito City, on Rosa
sp., 30 Oct. 2001, F. Freire, HAL) is morphologically also very close,
but the colonies occur on leaf spots and secondary ramoconidia
are up to 25 × 7 μm.
114. Cladosporium paracladosporioides Bensch, Crous &
U. Braun, Stud. Mycol. 67: 63. 2010. Figs 231, 232.
Holotype: Isol. by G.A. de Vries, deposited by Raistrick, No. 4079,
Sep. 1954 (CBS H-20442). Ex-type culture: CBS 171.54 = ATCC
11278 = 200943 = IFO 6369 = IMI 049626 = MUCL 917 = NCTC
4097.
Ill.: Bensch et al. (2010: 63–64, igs 52–53).
In vitro: Mycelium immersed and abundantly supericial; hyphae
sparingly branched, 1.5−5 μm wide, septate, not constricted at
septa, pale brown to medium brown or olivaceous-brown, septa
mostly quite regular, sometimes 2−3 in short succession, smooth to
asperulate or irregularly rough-walled, somewhat dimorphic, many
hyphae look like macronematous conidiophores but are not yet
sporulating, medium brown and smooth. Conidiophores macro-and
micronematous, arising terminally and laterally from ascending or
plagiotropous hyphae, erect, straight to mostly lexuous, sometimes
hardly distinguishable from hyphae. Macronematous conidiophores
cylindrical-oblong, non-nodulose, sometimes distinctly geniculatesinuous due to sympodial proliferation, once or several times,
unbranched or once branched, (17−)50−180(−300) × (2−)3−4 μm,
pluriseptate, sometimes slightly constricted at septa, pale to medium
brown or olivaceous-brown, smooth to irregularly asperulate or
loosely delicately verruculose, walls thickened. Conidiogenous
cells integrated, terminal, cylindrical-oblong, sometimes intercalary,
occasionally geniculate-sinuous, 8−40 μm long, with 2−4 apical loci
the genuS Cladosporium
or up to ive loci per cell, sometimes situated on lateral shoulders
or on short lateral prolongations at the apex, protuberant,
denticle-like, 1.5−2 μm diam, thickened and darkened-refractive.
Micronematous conidiophores narrower and paler, iliform,
unbranched, up to 165 μm long or even longer, 2−2.5(−3) μm wide,
subhyaline to pale brown. Conidiogenous cells integrated, terminal,
narrowly cylindrical-oblong, usually with a single apical locus,
sometimes with few loci. Ramoconidia occasionally formed, hardly
distinguishable from secondary ramoconidia. Conidia catenate, in
branched chains, up to four conidia in the unbranched terminal part
of the chain, straight, small terminal conidia obovoid, subglobose,
sometimes obpyriform, 4−7(−14) × 3−3.5 μm (av. ± SD: 5.3 ± 1.1
× 3.1 ± 0.2), aseptate, intercalary conidia limoniform, fusiform to
ovoid, 6−9(−11) × 3−3.5(−4) μm (av. ± SD: 7.7 ± 1.5 × 3.4 ± 0.3),
0(−1)-septate, with 1−2(−3) distal hila, secondary ramoconidia
ellipsoid, fusiform to subcylindrical, with up to four distal hila,
7−26(−30) × (3−)3.5−5 μm (av. ± SD: 16.2 ± 6.2 × 4.0 ± 0.4),
sometimes obclavate, up to 36 μm long, 0−3-septate, sometimes
slightly constricted at septa, irst septum median or often slightly
to distinctly in the upper half, sometimes in the lower, with 2−3
septa sometimes irregular, cells of different size, septa darkened,
pale brown, smooth or almost so to very inely asperulate, walls
somewhat thickened, attenuated towards apex and base, hila
protuberant, denticulate, broadly truncate, 0.8−2(−2.5) μm diam,
thickened and darkened-refractive; microcyclic conidiogenesis
occurring, forming secondary conidiophores.
Fig. 231. Cladosporium paracladosporioides (CBS 171.54). Dimorphic mycelium,
macro- and micronematous conidiophores, ramoconidia, conidia and microcyclic
conidiogenesis in vitro. Scale bar = 10 µm. K. Bensch del.
Culture characteristics: Colonies on PDA olivaceous-grey to irongrey and olivaceous-black, reverse greenish grey to olivaceousblack, woolly-luffy, margin colourless to white, glabrous to feathery,
very narrow, aerial mycelium luffy, loose, high, colonies somewhat
Fig. 232. Cladosporium paracladosporioides (CBS 171.54). A–C, E–F. Macronematous conidiophores and conidial chains. D, H. Conidial chains, septa of secondary ramoconidia
distinctly darkened. G. Microcyclic conidiogenesis. Scale bars = 10 µm.
www.studiesinmycology.org
207
BenSch et al.
shiny, without prominent exudates, sporulation profuse. Colonies
on MEA olivaceous-grey to greenish olivaceous due to profuse
sporulation, pale olivaceous-grey towards margins, somewhat
zonate, reverse olivaceous-grey, velvety to felty, margin narrow,
colourless to white, radially furrowed, colonies folded and wrinkled,
aerial mycelium abundant, loose to dense, without conspicuous
exudates, sporulating. Colonies on OA olivaceous-grey to irongrey, smoke-grey due to mycelium and sporulation, reverse
greenish black to leaden-grey, powdery to felty, margin colourless
to white, narrow, glabrous, aerial mycelium loose, diffuse to dense,
felty-luffy, without exudates, sporulating.
Substrate and distribution: Substrate and distribution unknown.
Notes: Cladosporium paracladosporioides is phylogenetically
close to C. varians (see Bensch et al. 2010, ig. 1, part a),
which is, however, easily distinguishable due to different
conidiophores, conidiogenous loci and conidia. Morphologically
C. paracladosporioides is rather similar to C. cladosporioides and
C. iranicum, but differs from the latter species in having shorter
conidial chains, wider terminal and intercalary, non-rostrate conidia
and wider, 0(−1)-septate secondary ramoconidia, and from C.
cladosporioides in having somewhat wider, often septate, smooth
or almost so to very inely asperulate conidia.
115. Cladosporium perangustum Bensch, Crous & U.
Braun, Stud. Mycol. 67: 65. 2010. Figs 233–235.
Holotype: South Africa, Pretoria, Walter Sisulu park, isol. from
Cussonia sp. (Araliaceae), 20 Feb. 2007, P.W. Crous (CBS
H-20451). Ex-type culture: CBS 125996 = CPC 13815.
Ill.: Bensch et al. (2010: 66−67, igs 54−56).
In vitro: Mycelium internal and supericial; hyphae iliform to
narrowly cylindrical-oblong, loosely branched, (0.5−)1−4 μm
wide, septate, sometimes slightly constricted at septa, sometimes
irregular due to intercalary swellings and constrictions, subhyaline
to pale olivaceous or pale olivaceous-brown, smooth to usually
verruculose or irregularly rough-walled, walls unthickened or almost
so, sometimes swollen at the base of conidiophores, sometimes
forming dense ropes. Conidiophores solitary, sometimes in pairs,
macronematous, semimacronematous or micronematous, arising
terminally and laterally from hyphae or from swollen hyphal cells,
erect, straight or slightly lexuous, iliform to narrowly cylindricaloblong, usually neither geniculate nor nodulose, sometimes
geniculate-sinuous or unilaterally slightly swollen at the apex,
unbranched, occasionally branched, once or several times,
branches short, peg-like or up to 30 μm long, conidiophores
(8−)12−130(−150) × (1.5−)2−3.5(−4) μm, 0−6 septate, usually not
constricted at septa, occasionally septa darkened, subhyaline,
pale olivaceous or pale olivaceous-brown, more or less roughwalled, especially towards the base of conidiophores, asperulateverruculose, at the apex smooth or almost so, walls unthickened
or slightly thickened, about 0.5 μm wide, sometimes slightly
attenuated towards the apex, at the base sometimes up to 4.5 μm
wide. Conidiogenous cells integrated, mainly terminal, sometimes
also intercalary or pleurogenous, narrowly cylindrical-oblong,
sometimes geniculate-sinuous, non-nodulose, in intercalary
cells loci situated on small peg-like lateral prolongations or just
208
below the septum, 7−40 μm long, with 1−4(−5) apically crowded
loci, forming clusters of pronounced scars, conspicuous,
subdenticulate to denticulate, 0.8−1.5 μm diam, thickened and
darkened-refractive. Ramoconidia cylindrical-oblong, 25−45 ×
2.5−3(−4.5) μm, aseptate, rarely 1(−2)-septate, base truncate,
2−2.5(−4) μm wide, sometimes slightly darkened or refractive.
Conidia numerous, catenate, in branched chains, branching in all
directions, 1−4 conidia in the terminal unbranched part of the chain,
small terminal conidia globose, subglobose or ovoid to obovoid,
2−4(−5) × (1.5−)2−2.5 μm (av. ± SD: 3.1 ± 0.6 × 2.1 ± 0.2), apex
broadly rounded or slightly attenuated, intercalary conidia ovoid,
limoniform to ellipsoid, somewhat fusiform or subcylindrical,
4−16(−19) × 2−3(−3.5) μm (av. ± SD: 8.7 ± 3.8 × 2.5 ± 0.4),
0(−1)-septate, attenuated towards apex and base, with 1−3(−5)
distal hila, secondary ramoconidia narrowly ellipsoid to cylindricaloblong, 6−30(−34) × 2−3(−3.5) μm (av. ± SD: 17.8 ± 7.4 × 2.5
± 0.4), 0−1(−3)-septate, septum median or often somewhat in the
upper half, with 2−4(−7) distal hila, pale olivaceous-brown, smooth
or almost so to inely verruculose (LM), under SEM smooth or
surface with somewhat irregularly reticulate structure or embossed
stripes probably caused by diminishing turgor and shriveling of
tender conidia, thin-walled, hila conspicuous, subdenticulate to
denticulate, (0.8−)1−1.5(−1.8) μm diam, thickened and darkenedrefractive; microcyclic conidiogenesis occasionally occurring.
Culture characteristics: Colonies on PDA attaining 33−76 mm
diam after 14 d, grey-olivaceous to olivaceous, olivaceous-grey or
iron-grey, sometimes with patches of smoke-grey or pale greenish
grey, reverse dull green, leaden-grey to olivaceous-grey, iron-grey
or olivaceous-black, sometimes releasing an olivaceous-buff or
orange to luteous soluble pigment into the agar, luffy, loccose or
powdery, margins glabrous to feathery, whitish, olivaceous-buff
or pale luteous due to the pigment, broad, regular or somewhat
undulate, aerial mycelium diffuse to loosely loccose or felty,
growth effuse, usually without prominent exudates, occasionally
numerous small to large prominent exudates formed, sporulation
profuse. Colonies on MEA reaching 40−72 mm diam after 14 d,
pale olivaceous-grey to glaucous-grey or grey-olivaceous, whitish
to smoke-grey due to aerial mycelium, reverse olivaceous-grey to
iron-grey, occasionally releasing an orange solube pigment into the
agar, velvety to loccose, margins white, narrow, regular to undulate,
glabrous to somewhat feathery, aerial mycelium abundantly formed,
covering most parts of colony surface, loosely to densely loccose
or felty, white to pale olivaceous-grey or smoke-grey, growth effuse
with sometimes elevated colony centre, radially furrowed, sometimes
few small prominent exudates formed, sporulation profuse. Colonies
on OA 40−75 mm diam after 14 d, whitish to smoke-grey and pale
olivaceous-grey or grey-olivaceous, reverse pale olivaceous-grey,
pale greenish grey to olivaceous-grey, leaden-grey or sometimes
amber-coloured due to the pigment released into the agar, velvety
or luffy to felty-loccose, margins colourless or greenish olivaceous,
glabrous, regular, aerial mycelium abundant, covering large parts
of the colony surface, dense, low to high, white, growth effuse,
sometimes few prominent exudates formed, sporulating.
Substrate and distribution: On plant material, ascomycetes,
isolated from food; widely distributed; Africa (South Africa), Asia
(India, Thailand), Australasia (Australia, New Zealand, Polynesia),
Europe (Germany), North America (USA).
Additional specimens examined: Sine loco, sine dato, isol. by C.H. Hassall, No.
4-1949, ident. by G.A. de Vries as C. cladosporioides (CBS 167.54 = ATCC 11276
the genuS Cladosporium
Fig. 233. Cladosporium perangustum (CBS 125996). Macro- and micronematous conidiophores, mycelium often formed in dense ropes, ramoconidia and conidial chains in
vitro. Scale bar = 10 µm. K. Bensch del.
Fig. 234. Cladosporium perangustum (CBS 125996). A–G. Macronematous conidiophores and conidial chains. Scale bar = 10 µm.
www.studiesinmycology.org
209
BenSch et al.
of Oncoba spinosa (Salicaceae), Sep. 2004, C.F. Hill (Hill 1076-1 = CPC 11663).
Polynesia, reserve Pun Kukui in forest, isol. from banana “Eka ulu”, 2006, coll. I.
Budenhagen, isol. P.W. Crous (CPC 12792, 12793). South Africa, Alkmar, Laeveld
Coop, isol. from wheat, 1988 (CPC 14008 = MRC 10135, as C. sphaerospermum);
Durban, botanical garden Durban near Reunion, -29.85, 31.0167, isol. from Strelitzia
sp. (Strelitziaceae), 2005, coll. W. Gams, isol. P.W. Crous (CPC 11806); Free State,
Danielsrus, isol. from oats, 1983 (CPC 14004 = MRC 03367); Transkei, Mazeppa
Bay, isol. from Strelitzia sp., growing on fruiting structures, 1 June 2008, P.W. Crous
(CPC 14911); Pretoria, Walter Sisulu park, isol. from Protea caffra (ascospore
isolate) (Proteaceae), 2 Jan. 2007, P.W. Crous (CPC 13730, 13774); isol. from
Teratosphaeria maculiformis (Teratosphaeriaceae) on Protea caffra, 2 Jan. 2007,
P.W. Crous (CPC 13727); Western Cape Province, Jonkershoek Nature Reserve,
isol. from Teratosphaeria ibrillosa (Teratosphaeriaceae), 30 Mar. 2007, P.W. Crous
(CPC 13870); Western Cape, Betties Bay, Harold Porter National park, isol. from
Protea cynaroides, 4 Dec. 2008, L. Mostert (CPC 15192). Thailand, isol. from
Acacia mangium (Fabaceae), 2005, coll. W. Himaman, isol. P.W. Crous (CPC 11526,
11856). USA, Louisiana, Baton Rouge, isol. from Magnolia sp. (Magnoliaceae), 8
Sep. 2007, P.W. Crous (CPC 14247); isol. from leaves of pecan tree, 8 Sep. 2007,
P.W. Crous (CPC 14256); Washington, Seattle, University of Washington campus,
isol. from chasmothecia of Phyllactinia guttata (Erysiphales) on leaves of Corylus
avellana (Betulaceae), 16 Sep. 2004, D. Glawe (CBS 126365 = CPC 11820, CPC
11815, 11819, 11821, 11831).
Notes: Cladosporium perangustum is probably a common and
widespread saprobic species isolated from a wide range of
substrates, which formed a well-supported, but genetically not quite
uniform clade in phylogenetic analyses (see Bensch et al. 2010,
ig. 1, part b), suggesting that this species has to be considered a
complex as in C. cladosporioides and C. pseudocladosporioides.
However, due to the morphological uniformity of the strains
concerned we tentatively prefer to maintain them as a single
species until more isolates will be available or additional characters
are to be found. Cladosporium exile and C. scabrellum are two
species morphologically comparable with C. perangustum but
genetically quite distinct (see Bensch et al. 2010, ig. 1, part b vs.
a). Cladosporium exile is clearly distinguished by its usually longer
and somewhat wider conidiophores, slightly wider ramoconidia
and conidia, shorter intercalary conidia and somewhat wider
conidiogenous loci and hila, and C. scabrellum differs in having
mainly macronematous and somewhat wider conidiophores and
slightly wider secondary ramoconidia.
116. Cladosporium phaenocomae Crous, Persoonia 26:
74. 2011. Fig. 236.
Fig. 235. Cladosporium perangustum (CBS 125996). A. Conidia with very gentle
surface ornamentation showing irregularly reticulate structures. B. A coherent view
on conidiophores, stipes, aerial hyphae and conidia. C. Secondary ramoconidia,
conidia and scars. The conidia at the upper right show some cell wall structures. D.
Conidiophore with secondary ramoconidia, intercalary and small terminal conidia.
Note the disruptions of the cell walls between the conidia. E. Scars on very elongated
secondary ramoconidia. F. Scar-pattern at the end of the conidiophores. Note the
lattened separation domes. G. Ropes of aerial hyphae. H. Running segmented
hyphae that may form conidiophores and not segmented aerial hyphae. Note the
blastoconidium on one hypha. Scale bars = 2 (A, C, E–F), 5 (D, G), 10 (B, H) µm.
= IMI 049624). Australia, isol. from margarine, N. Charley (CPC 11046); isol. from
Eucalyptus placita (Myrtaceae), coll. B.A. Summerell, isol. P.W. Crous (CPC 13686);
Northern Territory, Emerald Springs, S13°37’23, E131°36’40, isol. from Corymbia
foelscheana (Myrtaceae), 22 Sep. 2007, coll. B.A. Summerell, isol. P.W. Crous
(CPC 14566); isol. from Erythrophleum chlorostachys (Fabaceae), 9 Jan. 2007, B.A.
Summerell (CBS 126364 = CPC 14532). Germany, Essen, botanical garden, 51.45,
7.0167, isol. from Morus rubra (Moraceae), 2005, N. Ale-Agha (CPC 12216). India,
isol. from Eucalyptus sp. (Myrtaceae), 3 Jan. 2004, coll. W. Gams, isol. P.W. Crous
(CPC 11133); isol. from Musa sp. (Musaceae), 25 Oct. 2004, M. Arzanlou (CPC
11609). New Zealand, Auckland, Auckland University campus, isol. from leaves
210
Holotype: South Africa, Western Cape Province, Hermanus,
Fernkloof Nature Reserve, S 34°23’38’’E 19°16’9.7’’ on leaf bracts
of Phaenocoma prolifera (Asteraceae), 2 May 2010, K.L. Crous &
P.W. Crous (CBS H-20529). Ex-type cultures: CPC 18221, 18223
= CBS 128769.
Ill.: Crous & Groenewald (2011: 75, ig. 5)
In vitro: Mycelium immersed and supericial, abundant, 1–2.5
μm wide, septate, subhyaline to pale or medium olivaceousbrown, smooth to verruculose, at times forming hyphal ropes.
Macroconidiophores macronematous, solitary, arising terminally
and laterally from hyphae, erect, slightly flexuous, cylindricaloblong, 60–100(–200) × 2.5–3 μm, neither geniculate nor
nodulose, unbranched or branched below, 2–5-septate, not
constricted at septa, pale to medium olivaceous-brown, smooth.
Microconidiophores erect, intercalary, subcylindrical, 5–20 ×
2–3 μm, 0–1-septate, pale to medium brown, smooth to inely
verruculose. Conidiogenous cells integrated, terminal and
intercalary, cylindrical-oblong, neither geniculate nor nodulose,
the genuS Cladosporium
Fig. 236. Cladosporium phaeocomae (CBS 128769). A. Colony on MEA. B–H. A series of micro- and macronematous conidiophores showing conidia in chains. Scale bars =
10 µm.
5–20(–25) × (2–)3(–3.5) μm, with 1–4(–6) loci at the apex or 1–3
loci in intercalary cells with loci situated mostly all at more or less
the same level, conspicuous, subdenticulate, 1–1.5 μm diam,
somewhat thickened and darkened-refractive. Ramoconidia
occasionally formed, subcylindrical, 0(–1)-septate, 17–20(–
28) × (2–)3(–4) μm. Secondary ramoconidia fusoid-ellipsoid,
aseptate, (5–)10–15(–20) × (3–)3.5(–4) μm. Conidia pale to
olivaceous-brown, inely verruculose, catenate, in branched
chains, branching in all directions, up to 2–4 conidia in the
terminal unbranched part of the chain; intercalary conidia ovoid
to ellipsoid, aseptate, 4–5(–10) × (2.5–)3(–3.5) μm, with 1–3
distal hila, somewhat thickened, darkened-refractive, 1–1.5 μm
diam; small terminal conidia globose, subglobose to obovoid,
(3–)4(–5) × 2–3 μm, aseptate, rounded at the apex; microcyclic
conidiogenesis not observed.
Culture characteristics: Colonies after 1 wk at 25 °C in the dark,
with sparse aerial mycelium and smooth, even margins, reaching 7
cm diam; on OA greenish olivaceous; on MEA dull green (surface
and reverse); on PDA grey-olivaceous (surface), and olivaceousgrey in reverse; sporulating profusely on all media.
Substrate and distribution: On Phaenocoma; Australia.
Notes: Phylogenetically, C. phaenocomae is closely allied to C.
australiense (Bensch et al. 2010), but can be distinguished by
its narrower hyphae, conidia that are slightly roughened, and the
presence of microconidiophores (Crous & Groenewald 2011).
www.studiesinmycology.org
117. Cladosporium phlei (C.T. Greg.) G.A. de Vries, Contr.
Knowl. Genus Cladosporium: 49. 1952. Figs 237, 238.
Basionym: Heterosporium phlei C.T. Greg., Phytopathology 9: 580.
1919.
Holotype: USA, New York, Ithaca, greenhouse, on Phleum pratense
(Poaceae), 6 Apr. 1918, C.T. Gregory (CUP). Epitype (designated
here): Germany, Husum, isol. from Phleum pratense, isol. by
U.G. Schlösser, No. 155 (CBS H-20940). Ex-epitype culture: CBS
358.69.
Lit.: Matsushima (1975: 36), Ellis (1976: 334), Harada & Mino
(1976), Ellis & Ellis (1985: 508), David (1988e, 1997: 90), IMI
Distribution Maps of Plant Diseases 595 (1988), Ho et al. (1999:
139), Zhang et al. (2003: 142–143).
Ill.: Gregory (1919: 579, ig. 2), Matsushima (1975: 53, igs 1–2),
Ellis (1976: 335, ig. 254), Ellis & Ellis (1985: pl. 185, ig. 1904),
David (1988e: 1, ig.; 1997: 91, ig. 23), Ho et al. (1999: 141, igs
40–41), Zhang et al. (2003: 142, ig. 95).
In vivo: Leaf spots short ellipsoidal-fusiform, oblong, purple-violet to
brown, ochraceous, greyish, with narrow purple-violet margin, 1–4 ×
0.5–2 mm, sometimes conluent and larger. Colonies amphigenous,
mainly hypophyllous, inconspicuous, punctiform to subeffuse,
brown. Mycelium internal and external; hyphae septate, subhyaline
to pigmented, wall thin to somewhat thickened. Stromata lacking
or small, i.e., with small aggregations of swollen hyphal cells,
occasionally larger, up to 70 μm diam, brown, cells 3–10 μm wide.
Conidiophores solitary, in small groups or occasionally in fascicles,
211
BenSch et al.
throughout or tips somewhat paler, wall up to 1.5 μm thick, smooth
or almost so. Conidiogenous cells integrated, terminal, occasionally
intercalary, sympodial, with up to three coronate conidiogenous loci,
1.5–2.5 × 1 μm, somewhat protuberant. Conidia solitary, broadly
ellipsoid-ovoid, obovoid to short cylindrical, often somewhat broader
at the distal end, (10–)15–35(–55) × 7–14 μm, 0–3(–5)-septate,
usually not or barely constricted at the septa, pale to medium dark
brown, densely echinulate-verruculose, wall up to 1(–1.5) μm wide,
conidial cells sometimes with distinct lumen giving the impression
that the conidial walls are two-layered and rather thick-walled, apex
broadly rounded, base rounded to short obconically truncate, basal
hilum coronate, slightly protuberant, 1–2.5 × 1 μm.
Fig. 237. Cladosporium phlei (IMI 167692). Conidiophores and conidia in vivo. Scale
bar = 10 µm. U. Braun del.
arising from internal hyphae or stromatic cells, erect, straight,
subcylindrical to moderately geniculate-sinuous to subnodulose,
usually unbranched, 50–300 × 5–10 μm, pluriseptate, usually not
constricted at septa, pale to medium olivaceous-brown or brown
In vitro: Mycelium immersed and supericial; hyphae loosely
branched, 0.5–4(–5) μm wide, septate, without swellings and
constrictions, subhyaline to pale brown or olivaceous-brown,
smooth or almost so, walls unthickened or only slightly so,
narrow hyphae sometimes twisted. Conidiophores macro- and
micronametous, arising terminally from ascending hyphae and
laterally from plagiotropous hyphae, erect, straight to somewhat
lexuous, solitary. Macronematous conidiophores cylindricaloblong to irregular in outline in the upper portion due to distinct
sympodial proliferations and swellings, slightly swollen at tip,
subnodulose with loci situated on small lateral shoulders or
nodulose, swellings 5–8 μm diam, sometimes slightly to distinctly
geniculate, occasionally even zigzag-like, continuing growth in an
angle of 45°, unbranched or sometimes branched, 10–260 × (3–)
3.5–5(–6) μm, 0–10(–14)-septate, not constricted at septa, septa
sometimes not very conspicuous, pale to medium olivaceousbrown, smooth, occasionally verruculose, walls slightly thickened
to distinctly thick-walled, up to 1 μm wide. Conidiogenous cells
Fig. 238. Cladosporium phlei (CBS 358.69). A–F, H. Conidiophores being slightly to distinctly geniculate with solitary conidia or conidia arranged in short chains. G. Microcyclic
conidiogenesis. Scale bars = 10 µm.
212
the genuS Cladosporium
integrated, terminal and intercalary, cylindrical, subnodulose or
nodulose, slightly to distinctly geniculate-sinuous, with a single or
several loci, sometimes forming clusters of pronounced scars with
loci being situated on small lateral prolongations, subdenticulate
or denticulate, conspicuous, (1–)1.5–2 μm diam, thickened and
darkened-refractive. Micronematous conidiophores small, narrow,
often only as small lateral peg-like prolongations of hyphae,
short conical to cylindrical, mostly without swellings, sometimes
geniculate, unbranched, 5–30 × 1.5–2.5 μm, 0–2-septate,
subhyaline to pale brown, smooth, walls unthickened, often reduced
to conidiogenous cells, with a single or few apical loci, protuberant,
1–1.5 μm diam, thickened and darkened. Ramoconidia both formed
by macro- and micronematous conidiophores, cylindrical-oblong,
sometimes irregular due to swellings and sympodial proliferation,
16–50 × 3.5–5.5 μm, 0–3-septate, smooth or occasionally
verruculose, base broadly truncate, 2–3 μm wide. Conidia
catenate, solitary or in short unbranched or branched chains, small
conidia subglobose, ovoid, obovoid to broadly ellipsoid, 6.5–13 ×
4–7(–7.5) μm, 0(–1)-septate, apex broadly rounded, larger conidia
(solitary ones, intercalary conidia and secondary ramoconidia)
ellipsoid, broadly ellipsoid-ovoid, subcylindrical-oblong, sometimes
obclavate or clavate, 11–40 × 5–9(–10) μm, 0–1(–3)-septate,
usually not constricted at septa, septum median or somewhat in the
upper half, with age septa sometimes becoming sinuous, pale to
medium or dark olivaceous-brown or brown, surface ornamentation
variable ranging from smooth or almost so in young small terminal
conidia to verruculose, verrucose or distinctly echinulate, loose to
dense, verrucae up to 1 μm high, walls unthickened or somewhat
thickened, conidial cells sometimes with distinct lumen giving the
impression that the conidial walls are two-layered and rather thickwalled, apex broadly rounded, base rounded to short obconically
truncate, hila protuberant, sessile or subdenticulate, 1–2 μm diam,
thickened and darkened-refractive; microcyclic conidiogenesis
often occurring with conidia forming secondary conidiophores or
conidia germinating.
Culture characteristics: Colonies on PDA attaining up to 67 mm
diam after 2 wk, pale olivaceous-grey to olivaceous-black, reverse
fuscous-black, dark brick towards margins, releasing vinaceous
pigment into the agar, margins broad, feathery, regular, aerial
mycelium covering large parts of the colony, vinaceous-grey,
loccose, loose to dense, growth effuse with somewhat elevated
colony centre, without prominent exudates, not sporulating.
Colonies on MEA reaching 50–63 mm, whitish, smoke-grey to dark
vinaceous, reverse iron-grey to livid-red or vinaceous, releasing
vinaceous pigment into the agar, luffy, loccose, margins glabrous,
regular, aerial mycelium abundant, covering almost the whole
surface, luffy, loccose, dense, growth effuse, somewhat wrinkled,
without exudates, sporulating. Colonies on OA reaching 55–65 mm
diam, grey-olivaceous to olivaceous-grey and livid-red, reverse
livid-red to dark vinaceous, loccose, margins livid-red, glabrous,
regular, aerial mycelium pale vinaceous, loccose, growth lat,
without exudates, sporulating.
Co. Kildare, Dept. of Agric. Farm, on P. pratense, 17 Jul. 1972, A. Mangan (IMI
167692). Netherlands, Bennekom, isol. from Phleum sp., isol. by J. Florschütz-de
Waard (CBS 307.50). UK, England, Whitechurch, on P. pratense, 21 Jul. 1972, E.
Roberts (IMI 167525).
Notes: Zhang et al. (2003) described and illustrated this species
from China on Holcus sp. This record could not be proven. European
collections forming lesions on Calamagrostis cf. arundinacea
(Europe, Germany?, locality and date unclear, leg. Starcs, ex herb.
A. Ludwig, 13354, B 700006506) and Elymus arenarius (Germany,
Schleswig-Holstein, Sylt, Munkmarsch, 20 Aug. 1953, A. Ludwig,
B 700006492) are morphologically indistinguishable from C.
phlei. The biology of the collections concerned is quite unclear. It
is possible that C. phlei has a wider host range on various other
grasses. But it could also be that we have a complex of some
morphlogically similar, but biologically different species.
The choosen epitype well agrees with type material of
C. phlei. The species was originally described from North America
but an epitypiication with European material is justiied due to its
pathogenicity to the grass genus Phleum and its cosmopolitan
distribution.
118. Cladosporium phyllactiniicola Bensch, Glawe, Crous
& U. Braun, Stud. Mycol. 67: 67. 2010. Figs 239, 240.
Holotype: USA, Seattle, University of Washington campus,
47.6263, -122.3331, isol. from chasmothecia of Phyllactinia guttata
(Erysiphales) on leaves of Corylus avellana (Betulaceae), 2 Dec.
2004, D. Glawe (CBS H-20443). Ex-type cultures: CBS 126355
= CPC 11830, CBS 126352 = CPC 11836, CBS 126353 = CPC
11823, CBS 126354 = CPC 11825.
Ill.: Bensch et al. (2010: 68, igs 57−58).
In vitro: Mycelium immersed and supericial, plagiotropous,
ascending to erect, not dimorphic; hyphae sparingly branched,
Substrate and distribution: On Phleum pratense; Asia (China,
Japan, Korea), Australasia (New Zealand), Europe (Austria,
Byelorussia, Denmark, Estonia, Finland, Germany, Iceland,
Ireland, Netherlands, Norway, Poland, Russia, Sweden, UK), North
America (Canada; USA, widespread).
Additional specimens examined: Austria, Steiermark, Graz, Bezirk Mariatrost, on
Phleum pratense, 19 June 2008, C. Scheuer, ex herb. GZU (HAL 2243 F). Ireland,
www.studiesinmycology.org
Fig. 239. Cladosporium phyllactiniicola (CBS 126352). Macro- and micronematous
conidiophores, mycelium and conidial chains in vitro. Scale bar = 10 µm. K. Bensch
del.
213
BenSch et al.
Fig. 240. Cladosporium phyllactiniicola (CBS 126352). A–H. Macronematous conidiophores and conidial chains. Scale bars = 10 µm.
1−5(−6) μm wide, septate, not constricted at septa, sometimes
swollen, subhyaline to pale brown, minutely verruculose to
irregularly rough-walled, especially at the base of conidiophores,
sometimes forming ropes, often irregular in outline. Conidiophores
macro-and micronematous, arising laterally and terminally from
plagiotropous and ascending hyphae, erect, straight to slightly
lexuous. Macronematous and semimacronematous conidiophores
cylindrical-oblong, non-nodulose, sometimes geniculate towards
the apex, unbranched or once branched, 6−105(−120) × 2.5−5(−6)
μm, 0−6(−7)-septate, occasionally slightly constricted at septa,
pale to pale medium brown or olivaceous-brown, smooth or almost
so, walls unthickened in the younger conidiophores, thickened in
the older ones, sometimes slightly attenuated towards the apex.
Conidiogenous cells integrated, mainly terminal, cylindricaloblong, sometimes slightly geniculate, 6−25 μm long, with 2−4
conspicuous, subdenticulate to denticulate loci, sometimes
forming small clusters or situated on lateral shoulders formed due
to sympodial proliferation or on small lateral proliferations, loci
protuberant, 1−2 μm diam, thickened and darkened-refractive.
Micronematous conidiophores narrowly cylindrical-oblong to mostly
iliform, unbranched, non-geniculate and non-nodulose, often only
as short lateral outgrowths of hyphae, 8−40 × 2−2.5 μm, with few
septa, subhyaline, smooth, walls unthickened. Conidiogenous
cells integrated, terminal, 7−15 μm long, with a single locus or
up to three apical loci, 1−1.2 μm diam. Ramoconidia occasionally
formed, up to 28 μm long, 3.5−4 μm wide, base about 3 μm wide.
Conidia catenate, in branched chains, branching in all directions,
up to four conidia in the unbranched terminal part, straight, small
214
terminal conidia subglobose to obovoid, 3−6(−7) × 2−4 μm (av.
± SD: 4.2 ± 1.4 × 2.8 ± 0.8), aseptate, attenuated towards the
base, broadly rounded at the apex, intercalary conidia limoniform
to ellipsoid-ovoid, 5−10 × 3−4(−4.5) (av. ± SD: 6.2 ± 1.1 × 3.4 ±
0.6), 0(−1)-septate, secondary ramoconidia limoniform to usually
narrowly to broadly ellipsoid-ovoid to subcylindrical, 5−17(−24) ×
(2−)3−4.5 μm (av. ± SD: 11.8 ± 4.2 × 3.7 ± 0.7), conidia formed
by micronematous and semimacronematous conidiophores shorter
and narrower, 0−1(−2)-septate, mainly aseptate, not constricted at
septa, subhyaline to pale brown or pale olivaceous-brown, smooth
or almost so to inely asperulate, walls unthickened to slightly
thickened, often almost not attenuated towards apex and base,
hila conspicuous, subdenticulate to denticulate, 0.5−2 μm diam,
thickened and darkened-refractive; microcyclic conidiogenesis not
observed.
Culture characteristics: Colonies on PDA olivaceous-grey to irongrey, smoke-grey to pale olivaceous-grey due to aerial mycelium,
reverse leaden-grey, felty-woolly, margin narrow, white, somewhat
feathery, aerial mycelium sparse to abundant, felty, high, sometimes
few small, not very prominent exudates, formed, sporulating.
Colonies on MEA olivaceous-grey surface and reverse or greyolivaceous to greenish grey on surface, with patches of white or
smoke-grey due to dense abundant aerial mycelium, luffy, woolly,
margin narrow, white, glabrous to somewhat feathery, greenish
olivaceous at margins due to profuse sporulation, wrinkled,
sometimes radially furrowed, without exudates, sporulating.
Colonies on OA olivaceous-grey to grey-olivaceous or olivaceous,
the genuS Cladosporium
whitish to smoke-grey due to aerial mycelium, reverse leaden-grey,
olivaceous-grey to iron-grey, margin narrow, white, glabrous, aerial
mycelium absent, diffuse to dense, low to high, luffy to felty-woolly,
without prominent exudates, sporulating.
Substrate and distribution: Mycophilic, occurring on chasmothecia
of Phyllactinia guttata; USA.
Notes: Cladosporium phyllactiniicola, which was isolated from
ascomata of a powdery mildew, is morphologically comparable
with C. uredinicola, also known to occur on hosts of the
Erysiphales (Morgan-Jones & McKemy 1990, Heuchert et al.
2005), but genetically quite distinct (see Bensch et al. 2010,
ig. 1, part a vs. b) and morphologically easily separable by its
longer conidiophores, longer, 0−2-septate intercalary conidia and
longer, 0−3(−5)-septate secondary ramoconidia. Cladosporium
exile, also isolated from chasmothecia of Phyllactinia guttata and
comparable with C. phyllactiniicola, is morphologically clearly
distingusihed by having somewhat narrower conidiophores,
narrower terminal and intercalary conidia, 2−3 μm wide, and
longer and narrower secondary ramoconidia, 7−25(−35) ×
2.5−3.5(−4) μm.
119. Cladosporium phyllophilum McAlpine, Agric. Gaz.
New South Wales 7: 153. 1896. Figs 241–243.
Lectotype (designated by Heuchert et al. 2005): Australia,
Victoria, Armadale, on leaves and twigs of Prunus persica
(= Persica vulgaris) (Rosaceae) infected with and deformed by
Taphrina deformans (= Exoascus deformans), 16 Feb. 1896,
D. McAlpine (VPRI 2490). Epitype (designated in Bensch et al.
2010): Germany, Sachsen-Anhalt, Halle (Saale), botanical garden,
isolated from fruits of Prunus cerasus infected with Taphrina sp.,
2004, K. Schubert (CBS H-20444). Ex-type culture: CBS 125992
= CPC 11333.
Fig. 241. Cladosporium phyllophilum (VPRI 2490). Erect conidiophores arising from
stromata, conidiogenous cells, ramoconidia and conidia in vivo. Scale bar = 10 µm.
B. Heuchert del.
= Cladosporium exoasci Ellis & Barthol., in Shear, Fungi Columb., Cent. XV,
No. 1493. 1901, nom. nud. [syntypes: B 700006330, NY].
= Cladosporium exoasci Lindau, in Rabenhorst, Krypt.-Fl., ed. 2, 1(8): 808.
1907. [lectotype (designated by Heuchert et al. 2005): Jaap, Fungi Sel. Exs.
248 (B 700006337); isolectotypes: Jaap, Fungi Sel. Exs. 248, e.g. HAL].
Lit.: McAlpine (1902: 100), Saccardo (1906: 575, 1913a: 1370),
Lindau (1910: 796), Braun (2001: 53–56), Heuchert et al. (2005:
36–40), Bensch et al. (2010: 69–71).
Ill.: McAlpine (1902: igs 87–88), Braun (2001: 55, ig. 1), Heuchert
et al. (2005: 37–38, igs 13–14, pl. 2, fig. 12), Bensch et al. (2010:
70, ig. 59).
Exs.: Braun, Fungi Sel. Exs. 50, 51, 80; Jaap, Fungi Sel. Exs. 248;
Petrak, Fl. Bohem. Morav. Exs. Pilze 556; Shear, Fungi Columb.
1493; Sydow, Mycoth. Germ. 1780; Triebel, Microf. Exs. 534.
In vivo: Colonies on fruits, leaves, rarely also twigs, usually on
deformations caused by Taphrina spp., olivaceous-brown to dark
brown, occasionally yellowish brown or greyish olivaceous, loose to
dense, conluent, caespitose, effuse, velvety. Mycelium immersed
and external, supericial; hyphae creeping, interwoven, branched,
2–7(–10) μm wide, cells 4–15 μm long, septate, often constricted
at the septa, with swellings, olivaceous-brown or pale brown,
occasionally subhyaline. Stromata diffuse, immersed, loose to
dense, conluent, composed of swollen hyphal cells, subglobose,
www.studiesinmycology.org
Fig. 242. Cladosporium phyllophilum (B 700006334 and B 700006335,
lectoparatypes of C. exoasci Lindau). Erect conidiophores arising from stromata,
creeping conidiophores, conidiogenous cells, ramoconidia and conidia. Scale bar =
10 µm. B. Heuchert del.
215
BenSch et al.
Fig. 243. Cladosporium phyllophilum (CBS 125992). A–H. Macronematous conidiophores and conidial chains in vitro. I–K. SEM photos showing the smooth or somewhat
shrivelling surface ornamentation of conidia and details of the coronate scar structure. Scale bars = 5 (I–K), 10 (A–H) µm.
2–13 μm diam, olivaceous to medium brown, smooth, walls slightly
thickened. Conidiophores solitary, arising from hyphae or swollen
hyphal cells, or in loose to dense groups, arising from stromata,
erect to decumbent, creeping, straight to curved, geniculatesinuous, simple to often multibranched, (6–)20–233(–250) × 3–8
μm, occasionally even longer, sometimes swollen at the base, 7–11
μm wide, densely 4–14-septate, cells 10–15 μm long, usually not
constricted at the septa, olivaceous to medium brown, occasionally
paler, usually paler towards the apex, sometimes subhyaline
at the tip, smooth to faintly rough-walled, wall slightly thickened,
above all near the base, but thin-walled towards the apex, rarely
with enterogenous, monopodial proliferation. Conidiogenous cells
integrated, terminal and intercalary, occasionally pleurogenous,
subcylindrical, 7–63 μm long, polyblastic, proliferation sympodial,
conidiogenous loci protuberant, 1.5–3 μm diam, thickened,
216
darkened. Ramoconidia clavate, ellipsoid, subcylindrical, fusiform,
13–35(–38) × 3–10 μm, (0–)1–5(–6)-septate, occasionally
constricted at the septa, base truncate to convex, 2–3.5 μm wide,
without a cladosporioid hilum. Conidia usually in branched chains,
straight to slightly curved, subglobose, ellipsoid-ovoid, obovoid,
3.5–18(–26) × 2–6(–7) μm, 0–1-septate, usually without any
constrictions, subhyaline to pale brown, smooth, walls unthickened
to slightly thickened, apex rounded to somewhat attenuated,
with up to ive hila, base truncate to convex, occasionally slightly
attenuated, hila protuberant, (0.5–)1–2.5 μm diam, thickened and
darkened; microcyclic conidiogenesis occurring.
In vitro: Mycelium immersed to supericial; sparingly branched,
1−4 μm wide, septate, not constricted at septa, pale, almost
hyaline to olivaceous-brown or brown, smooth to irregularly
the genuS Cladosporium
rough-walled, verruculose or verrucose with large wart-like
structures, walls thin or slightly thickened. Conidiophores
semimacronematous or macronematous, solitary, arising
laterally or terminally from plagiotropous or ascending hyphae.
Macronematous conidiophores erect to decumbent, straight or
lexuous, cylindrical-oblong, unbranched or branched, usually
once, sometimes twice or up to four times, branches often start as
short lateral outgrowth just below a septum, becoming longer with
age, up to 65(−90) μm long, often at an angle of 45°, sometimes
up to 90°, neither nodulose nor geniculate, 15−180 × 4−5(–6) μm,
pluriseptate, not constricted at septa, pale olivaceous to medium
olivaceous-brown or brown, smooth to sometimes asperulate,
especially towards the apex, walls somewhat thickened, up to 1
μm wide, base sometimes also covered by wart-like structures.
Conidiogenous cells integrated, terminal or intercalary, cylindricaloblong, sometimes slightly geniculate towards the apex, 13−41
μm long, mostly with up to three or four subdenticulate protuberant
loci, sitting close together at the apex, 1.5−2 μm diam, thickened
and darkened-refractive. Semimacronematous conidiophores
paler, smaller and narrower, unbranched or branched once or
twice, 15−100 × 2−3 μm, septate, conidiogenous cells 7−19 μm
long, with up to seven distal scars, subdenticulate, crowded at
the apex, hila 1−1.5 μm diam. Ramoconidia occasionally formed
(hardly distinguishable from secondary ramoconidia), cylindricaloblong, 17−33 × 4 μm, aseptate, sometimes 1-septate, pale
olivaceous, smooth, walls unthickened or almost so, base
truncate. Conidia numerous, catenate, in branched chains,
branching in all directions, 1−3 conidia in the terminal unbranched
part of the chain, small terminal conidia obovoid to ovoid, 3−7 ×
2−3 μm (av. ± SD: 4.8 ± 1.4 × 2.5 ± 0.3), aseptate, hila 0.5−0.8
μm diam, intercalary conidia ovoid, ellipsoid-ovoid, 6−13 ×
3−4 μm (av. ± SD: 8.9 ± 2.1 × 3.2 ± 0.3), aseptate, with up to
4(−5) distal hila, hila 0.8−1.2 μm diam, secondary ramoconidia
ellipsoid, subcylindrical to cylindrical, 7−32 × 2.5−4(−5) μm (av.
± SD: 18.7 ± 7.2 × 3.7 ± 0.6), 0(−2)-septate, not constricted at
septa, subhyaline, pale olivaceous, smooth or almost so, with
1−4 distal hila, walls unthickened, hila 1−2.2 μm diam, thickened
and darkened-refractive; conidia formed by semimacronematous
conidiophores shorter, paler and narrower.
Culture characteristics: Colonies on PDA attaining 41−46 mm
diam after 1 mo, grey-olivaceous to olivaceous, reverse iron-grey
to greyish blue, powdery to felty, margin white, narrow, glabrous,
regular, aerial mycelium diffuse to loose, luffy, mainly in colony
centre, growth lat, without prominent exudates, sporulation profuse.
Colonies on MEA reaching 49−52 mm diam after 1 mo, smoke-grey
to olivaceous-grey or brownish, whitish towards margins, reverse
olivaceous-grey, velvety to woolly, margin white, glabrous, radially
furrowed, aerial mycelium sparse to more abundantly formed, luffy,
few prominent exudates formed, sporulation profuse. Colonies
on OA reaching 44−49 mm diam after 1 mo, grey-olivaceous to
olivaceous or olivaceous-grey, reverse leaden-black to leaden-grey,
powdery to luffy, margin white, narrow, glabrous, aerial mycelium
sparse, diffuse to more abundantly formed in colony centre, high,
luffy-felty, without prominent exudates, sporulation profuse.
Substrate and distribution: On species of Taphrina, including
T. cerasi, T. communis, T. deformans and T. pruni on Prunus s.
lat. species; Asia (Armenia, Georgia, Kazakhstan, Uzbekistan),
Australia, Europe (Czech Republic, France, Germany, Romania,
Switzerland), North America (USA) – on Taphrina cerasi on Prunus
cerasus (Germany, Kazakhstan), T. communis on P. americana
www.studiesinmycology.org
(USA, CO), T. deformans on P. persica (Australia, Germany,
Uzbekistan), T. pruni on P. americana (USA, WI ?), T. pruni on P.
avium (Switzerland), T. pruni on P. cerasus (Germany), T. pruni
on P. domestica (Germany, Kazakhstan), T. pruni on Prunus sp.
(Armenia, France, Czech Republic), ?Taphrina sp. on Malus ×zumi
(Germany), Taphrina sp. on P. armeniaca (Australia), Taphrina sp.
on P. persica (Australia), Taphrina sp. on P. spinosa (Germany,
Romania), Prunus avium, P. cerasus and P. domestica (Lithuania),
host unknown (Georgia). On Teratosphaeria proteae-arboreae;
South Africa.
Additional specimens examined: Czech Republic, Mähren, Eisgrub, Grenzteiche,
on Taphrina pruni (Taphrinaceae), 7 Jun. 1911, H. Zimmermann, Petrak, Fl. Bohem.
Morav. Exs. Pilze 556 (HBG, M-0057605). France, Lothringen, Forbach, Kreuzberg,
on T. pruni on Prunus domestica (Rosaceae), 25 Jun. 1916, A. Ludwig (B 700006333).
Germany, Bavaria, on P. spinosa, Jun. 1909, A. Vill (HBG); on T. deformans on
P. persica, Jun. 1909, A. Vill (HBG); Gerolzhofen, on T. pruni on P. domestica, 7
Jul. 1909, A. Vill (HBG); Kr. Freising, Freising/Weihenstephan, on Taphrina sp. on
Prunus sp., Jun. 1918, Bons (B 700006336); Brandenburg, Kr. Prignitz, Triglitz, on
T. pruni on P. domestica, 1 Oct. 1904, O. Jaap (B 700006335); Rangsdorf near
Zossen, on T. cerasi on P. cerasus, 24 Jun. 1919, H. Sydow, Sydow, Mycoth.
Germ. 1780 (PH); He-Nassau, Dillkreis, Donsbach, on T. pruni on P. domestica, 21
Jun. 1936, A. Ludwig (B 700006331); Rhön, near Gersfeld, ca. 500 m, on T. pruni
(= Exoascus rostrupianus) on P. spinosa, 31 Jul. 1906, O. Jaap, Jaap, Fungi Sel.
Exs. 248 (B 700006327, lectotype of C. exoasci Lindau, isolectotypes: Jaap, Fungi
Sel. Exs. 248); Sachsen, Kirchberg, Alte Hartmannsdorfer Str., garden Bensch, on
T. pruni on P. domestica, 25 Jul. 2004, K. Schubert (HAL) and Braun, Fungi Sel.
Exs. 50; Sachsen-Anhalt, Halle, botanical garden, on T. pruni on P. cerasus, 11 Jun.
2004, B. Heuchert (HAL 1823); on ?Taphrina sp. on Malus ×zumi, 14 Jun. 2004, U.
Braun, Braun, Fungi Sel. Exs. 51 (HAL); Osterhausen, Freiplatz 14, in a garden, on
Taphrina sp. on Prunus padus, 9 Jul. 2006, K. Schubert, U. Braun, Fungi Sel. Exs.
80 (HAL). South Africa, Western Cape Province, Jonkershoek Nature Reserve,
on Teratosphaeria proteae-arboreae (Teratosphaeriaceae) on Protea nitida [= P.
arborea] (Proteaceae), 30 Mar. 2007, P.W. Crous, CPC 13873. Switzerland, Kt.
Bern, Berner Oberland, Kandersteg, on Taphrina pruni on Prunus avium, 19 Jul.
1905, O. Jaap, Jaap, Fl. Schweiz 16 (B 700006334, syntype of C. exoasci Lindau).
USA, Colorado, Walsenberg, on T. communis on P. americana, Jul. 1900, C.L.
Shear, Shear, Fungi Columb. 1493 (B 700006330, syntype of C. exoasci Ellis &
Barthol.); Pope (WI ?), on T. pruni on P. americana, 1 Jul. 1929 (NY)
Notes: Cladosporium phyllophilum occurs on woody host plants
usually associated with Taphrina species. A South African strain
isolated from Teratosphaeria proteae-arboreae clustered together
with the epitype [on ACT the two strains are 98 % identical (217/220
bases), whereas on TEF only 96 % (374/387 bases and 4 gaps)
(see Bensch et al. 2010)], which is, however, morphologically
slightly different by its shorter, mostly unbranched conidiophores
and shorter, less-septate, smooth to asperulate conidia. The South
African fungus is only tentatively assigned to C. phyllophilum. It
possibly represents a cryptic species, but additional collections
are necessary to prove its identity. Cladosporium phyllophilum is
phylogenetically close to C. licheniphilum and C. phyllactiniicola (see
Bensch et al. 2010, ig. 1), but morphologically clearly separable by
several differences in the characters of conidiophores and conidia
(see Bensch et al. 2010). Heuchert et al. (2005) pointed out that C.
phyllophilum represents the oldest name for this species that was
previously usually known as C. exoasci. In the type collection of
C. exoasci (B 700006336), with relatively short [14−99 × 3−5(−6)
μm], unbranched, non-decumbent conidiophores, the otherwise
characteristic dimorphism of the conidia could not be observed,
and typical ramoconidia were lacking, but the designated epitype
agrees well with the description given in Braun (2001) and Heuchert
et al. (2005), although conidiophore and conidial measurements
are slightly narrower in vitro than on the natural host.
A collection recently found on fruits of the unusual host Malus
×zumi in Germany (HAL) is morphologically indistinguishable
from C. phyllophilum. Infections of the fruits by Taphrina could not
217
BenSch et al.
be proven with certainty. Valiuškaitė (2002) recorded C. exoasci
from Lithuania on plum, sweet and sour cherry, but without any
reference to Taphrina.
120. Cladosporium pini-ponderosae K. Schub., Gresl. &
Crous, Persoonia 22: 118. 2009. Figs 244–246.
Holotype: Argentina, Neuquén, Aluminé, Lagos Marmol property,
39°22’52’’S, 71°5’38’’W, on needles of Pinus ponderosa (Pinaceae),
Jan. 2005, A. Greslebin (CBS H-20210). Isotypes: BAFC 51696,
HAL 2322 F. Ex-type cultures: CBS 124456 = CIEFAP 322 = CPC
13980.
Ill.: Schubert et al. (2009: 118–119, igs 7–9).
In vivo: Isolated from needles becoming necrotic from the top to
the base, no discrete leaf lesions formed. Colonies punctiform,
sometimes coalescing, dark brown, distributed along the stomatal
lines in upper and under sides of the needles but more abundant
on the lower side. Mycelium internal, immersed, but also external,
supericial, composed of septate, smooth, subhyaline to pale
brown, thin to slightly thick-walled hyphae, 2.5–6(–8) μm diam,
hyphae somewhat constricted at septa, often swollen, forming
substomatal, pseudoparenchymatous stromata, small to extended,
50–135 μm diam or even larger, several layers deep, composed
of thick-walled, olivaceous to brown, rounded cells, 8–15 μm
diam. Conidiophores in small to large dense fascicles, arising
from stromata, emerging through stomata, subcylindrical, sinuous,
slightly geniculate due to sympodial proliferation and slightly
tapered towards the apices, mostly unbranched, rarely branched,
12–70(–100) × 3.5–8 μm, 0–3-septate, rarely forming more septa,
pale to dark olivaceous-brown, paler towards apices, walls brown,
thick-walled, often 2-layered (two distinct wall layers visible),
darkened and thickened towards the base, often enteroblastically
proliferating, once or twice. Conidiogenous cells integrated, mostly
terminal but also intercalary, subhyaline to pale brown, geniculate,
polyblastic, proliferation sympodial with several conidiogenous
loci situated terminally or laterally on small shoulders, cicatrised,
loci protuberant, denticulate, thickened and darkened-refractive.
Conidia single or catenate, in unbranched or branched chains,
ovoid, ellipsoid to subcylindrical, 5–20(–31) × (3–)4–8(–9) μm
(av. ± SD: 13.1 ± 5.9 × 5.7 ± 1.5), 0–3-septate, pale brown to
brown, slightly thick-walled, almost smooth to usually verruculose,
sometimes verrucose, hila conspicuous, 1–2 μm diam, thickened
and darkened-refractive; microcyclic conidiogenesis observed.
In vitro: Mycelium immersed and supericial; hyphae unbranched
or loosely branched, 1.5–5(–8) μm wide, septate, without any
constrictions or swellings, subhyaline to pale greyish brown or
dingy-brown, smooth to irregularly rough-walled to verruculose or
walls covered by polysaccharide-like material, walls unthickened
or only slightly thickened. Conidiophores macronematous,
arising terminally from ascending or laterally from plagiotropous
hyphae, solitary, sometimes in pairs of two, erect, straight or
slightly lexuous, subcylindrical to cylindrical-oblong, sometimes
slightly geniculate towards the apex, once or twice, unbranched,
sometimes once branched, rarely twice, non-nodulose, 14–190
× (2.5–)3.5–5.5 μm, septate, not constricted at septa, branches
as short lateral outgrowths just below a septum, later becoming
longer, greyish brown or dingy brown, sometimes paler towards the
apex, almost smooth to minutely verruculose to irregularly roughwalled, walls thickened, 0.5–1(–1.5) μm wide, sometimes even
appearing to be 2-layered (two distinct wall layers visible), not or
only very slightly attenuated towards the apex. Conidiogenous cells
integrated, mainly terminal, sometimes intercalary, subcylindrical to
cylindrical-oblong, sometimes slightly geniculate towards the apex,
14–45 μm long, conidiogenous loci mostly crowded at the apex,
(1–)2–4(–6) loci, broadly truncate, central convex dome not very
prominent, 1.5–2.5 μm diam, somewhat thickened and darkened-
Fig. 244. Cladosporium pini-ponderosae (BAFC 51696). Fascicle of conidiophores arising from extended stromata and conidia in vivo. Scale bar = 10 µm. A. Greslebin del.
218
the genuS Cladosporium
Fig. 245. Cladosporium pini-ponderosae (CBS 124456 = CPC 13980).
Conidiophores and conidia in vitro. Scale bar = 10 µm. K. Bensch del.
refractive. Ramoconidia cylindrical-oblong, not attenuated
towards the base, 20–45 × 3.5–5(–5.5) μm, 0–1(–3)-septate,
not constricted, concolorous with the tips of conidiophores, walls
thickened, base unthickened, broadly truncate. Conidia catenate,
in unbranched or branched chains, branching in all directions, up
to nine conidia in the terminal chain, small terminal conidia obovoid
to ellipsoid-ovoid, 5–6 × 2.5–4(–4.5) μm (av. ± SD: 5.5 ± 0.5 × 3.3
± 0.6), broadly rounded at the apex, slightly attenuated towards
the base, aseptate, hila 0.5–1(–1.2) μm diam, intercalary conidia
ellipsoid-ovoid, fusiform, sometimes rostrate towards the distal
end, attenuated towards apex and base, 6–15(–22) × 3–4(–5) μm
(av. ± SD: 9.9 ± 3.8 × 3.8 ± 0.5), aseptate, rarely 1-septate, mostly
with a single but sometimes up to three distal hila, hila 0.8–1.5 μm
diam, secondary ramoconidia fusiform to subcylindrical, 10–30(–
36) × 3.5–5 μm (av. ± SD: 20.3 ± 6.4 × 4.3 ± 0.4), 0–1(–2)-septate,
very rarely 3-septate, not constricted at septa, septa sometimes
slightly darkened, becoming somewhat sinuous with age, with
up to four distal hila, attenuated towards apex and base, pale to
medium greyish brown or dingy brown, small terminal conidia and
young conidia subhyaline, verruculose to very irregularly roughwalled, younger conidia almost smooth, walls appear to be very
thick-walled, lumen distinct, very pale between inner and outer wall,
hila broadly truncate, 0.5–2.5 μm diam, somewhat thickened and
darkened-refractive; microcyclic conidiogenesis occurring.
Culture characteristics: Colonies on PDA attaining 65–73 mm
diam after 1 mo, grey-olivaceous to olivaceous-grey, reverse
olivaceous-grey to iron-grey, velvety to powdery, margin white,
Fig. 246. Cladosporium pini-ponderosae (CBS 124456 = CPC 13980). A–D. Conidiophores with conidial chains. E–G. Conidia. Scale bars = 10 µm.
www.studiesinmycology.org
219
BenSch et al.
narrow, glabrous to feathery, regular, entire edge to slightly
undulate, aerial mycelium formed, pale olivaceous-grey, somewhat
luffy, especially in colony centre, growth lat, without conspicuous
exudates, sporulation profuse. Colonies on MEA reaching 64–74
mm diam after 1 mo, olivaceous-grey to grey-olivaceous due to
profuse sporulation, greenish grey towards the margin, velvety,
margin white, narrow, regular, glabrous, radially furrowed, growth
lat, often folded, without conspicuous exudates.
Substrate and distribution: On Pinus ponderosa; Argentina.
Notes: Cladosporium pini-ponderosae is genetically close to
C. chubutense, which has also been described from dead and
living needles of Pinus ponderosa collected in pine plantations
in Argentina (Patagonia). ITS data of the two species are almost
identical, but ACT and TEF sequence are distinct (see Schubert et
al. 2009, igs 1, 3, table 3), and C. chubutense is distinguished from
C. pini-ponderosae by forming both macro- and micronematous
conidiophores in culture which are slightly to distinctly geniculate
towards the apex and somewhat narrower in vitro [(1.5–)2.5–4 μm
vs (2.5–)3.5–5.5 μm in C. pini-ponderosae] and conidia with quite
distinct surface ornamentation formed in much shorter chains.
Pinus ponderosa is not native to Argentina, so the fungus could
have been imported with the pine or jumped onto the pine from a
different host.
121. Cladosporium pipericola R.A. Singh & Shankar,
Mycopathol. Mycol. Appl. 43(1): 110. 1971, as “pipericolum”.
Figs 247, 248.
Lectotype (designated here): India, Uttar Pradesh, Varanasi, on
living leaves of Piper betle (Piperaceae), 15 Jan. 1965, G. Shankar
(IMI 116933). Isolectotype: MSP, No. 342.
Lit.: David (1988d), Schubert (2005b: 120–122).
Ill.: Singh & Shankar (1971: 111, pl. 1, igs 3–4), David (1988d: 1,
ig.), Schubert (2005b: 121, ig. 56, pl. 22, igs D–F).
In vivo: Leaf spots irregular in shape, 20–100 × 15–30 mm wide,
brown, with characteristically zonate ring formation, starting
on the lower leaf surface, but eventually affecting both sides.
Colonies usually hypophyllous, rarely epiphyllous, pale greenish
to olivaceous-brown, villose. Mycelium external, supericial;
Fig. 247. Cladosporium pipericola (IMI 116933). Symptoms, stromata (textura
angularis), conidiophores and conidia in vivo. Scale bar = 10 µm. K. Bensch del.
hyphae branched, 1–3(–4) μm wide, septate, not constricted at the
septa, pale brown, smooth or rarely slightly rough-walled, walls
slightly thickened, forming stromata by aggregation. Stromata
(40–)50–80(–100) diam, composed of more or less isodiametrical
brown cells, (5–)7–9.5(–12) μm wide, forming a textura angularis.
Conidiophores solitary, arising from creeping hyphae, or in small
loose groups, arising from stromata, erect, straight to slightly
lexuous, unbranched or often branched, at the base and, above
all, towards the apex, 15–300 × 2.5–8 μm, septate, not constricted
at the septa, pale olivaceous to olivaceous-brown, thick-walled,
verruculose and wider at the base, becoming thin, smooth-
Fig. 248. Cladosporium pipericola (IMI 116933). A. Symptoms. B, C. Conidiophores and conidia. Scale bars = 10 (B–C) µm.
220
the genuS Cladosporium
walled and attenuated towards the apex, sometimes more or less
nodulose with small swellings which are not connected with the
conidiogenesis. Conidiogenous cells integrated, mostly terminal,
cylindrical, 14–35 μm long, proliferation sympodial, with a single or
only few conidiogenous loci, protuberant, subdenticulate, truncate
to slightly convex, 1–2.5 μm diam, thickened, somewhat darkenedrefractive. Conidia catenate, in branched chains, straight to slightly
curved, variable in shape and size, obovoid, ellipsoid, limoniform,
fusiform, cylindrical, (3–)4.5–21(–28.5) × (1.5–)3–6.5(–8.5) μm,
0–2(–5)-septate, not constricted at the septa, pale olivaceousbrown to olivaceous-brown, smooth, sometimes distinctly verrucose
with age, walls unthickened to slightly thickened, apex rounded or
with up to four apical hila, truncate or slightly convex at the base,
hila protuberant, 0.5–2(–2.5) μm diam, thickened and somewhat
darkened-refractive; microcyclic conidiogenesis not observed.
Substrate and distribution: On Piper betle; India.
Notes: Infection starts at the tips or margins of the leaves and
spreads over main parts of the leaf lamina. Lesions develop fast
under favourable conditions (cold damp weather) and soon cover
the entire leaf surface causing premature defoliation. Infection
symptoms initially are evident in December becoming severe in
January and February (Singh & Shankar 1971).
Cladosporium pipericola is morphologically closely allied to
C. cladosporioides but differs, in having wider, often branched
and sometimes nodulose conidiophores and 0–2(–5)-septate,
somewhat wider conidia.
122. Cladosporium pisicola W.C. Snyder, Phytopathology
24: 899. 1934. Fig. 249.
≡ Cladosporium cladosporioides f. sp. pisicola (W.C. Snyder) G.A. de
Vries, Contr. Knowl. Genus Cladosporium: 61. 1952.
Neotype (designated here): USA, California, Berkeley, on pods
of Pisum sativum (Fabaceae), 20 Oct. 1940, W.C. Snyder, herb.
Dearness 2395 (DAOM).
Lit.: Farr et al. (1989: 629), Bensch et al. (2010: 34).
Ill.: Snyder (1934: 893, ig. 2).
In vivo: Lesions on living pods and seeds, subcircular in outline
to irregularly shaped, occasionally oblong, 1–8 mm diam, brown,
later with paler centre, pale brown, straw-coloured, dingy grey,
margin darker brown. Colonies conined to lesions, effuse, brown.
Mycelium internal. Stromata lacking. Conidiophores solitary,
occasionally loosely grouped, arising from internal hyphae or
small hyphal aggregations, erect, straight, subcylindrical to
distinctly geniculate-sinuous, occasionally branched, 20–180
× 3–5(–7) μm, continuous to usually septate, pale olivaceous to
olivaceous-brown, smooth, wall thin, < 1 μm. Conidiogenous cells
integrated, terminal and intercalary, 10–30 μm long, often distinctly
geniculate, conidiogenous loci few to usually numerous, often
densely aggregated, 1–2.5 μm diam, sometimes protuberant,
subdenticulate. Conidia in simple or branched chains, with up to
six terminal hila, smaller conidia 3–10 × 2–5 μm, 0(–1)-septate,
secondary ramoconidia 8–26 × 3–5(–7) μm, 0–1(–3)-septate,
pale olivaceous to olivaceous-brown, smooth, thin-walled, < 1
μm, hila 1–2 μm diam, often somewhat protuberant; microcyclic
conidiogenesis not observed.
www.studiesinmycology.org
Fig. 249. Cladosporium pisicola (DAOM). Conidiophores and conidia in vivo. Scale
bar = 10 µm. U. Braun del.
Substrates and distribution: On Pisum sativum; North America
(USA, California).
Additional specimens examined: USA, California, Berkeley, on seeds of Pisum
sativum, 20 Jul. 1940, W.C. Snyder, herb. Dearness 2395 (DAOM); isolated from
infected pods of Pisum sativum, Dec. 1940, W.C. Snyder (BPI 427394, 427395;
DAOM 41245; WIS); Santa Cruz Co., Watsonville, on pods of Pisum sativum, 22
Nov. 1943, Ramsey & Cameron 253 (BPI 427396).
Notes: Snyder (1934), who introduced the name C. pisicola for a
plant pathogenic leaf, stem and pod spot disease, confused this
species with C. cladosporioides, which is rather common on the
phyllosphere of pea. Therefore, it is not surprising that several
original strains, including CBS 144.35, which is an authentic culture
of W.C. Snyder from 1935, proved to belong to C. cladosporioides
based on morphology and molecular sequence analyses. This is
also the reason for the decision of de Vries (1952) who reduced
C. pisicola to synonymy with C. cladosporioides. Cladosporium
pisicola was based on heterogeneous elements, i.e. cultures of
C. cladosporioides and in vivo material with a morphologically
distinct leaf spotting Cladosporium. Thus, the application of the
name C. pisicola depends on its typiication. Since C. pisicola
is a leaf-spotting, obviously biotrophic fungus, morphologically
clearly distinct from C. cladosporioides by its distinctly sympodially
proliferating, geniculate conidiophores with terminal as well as
intercalary conidiogenous cells and numerous, often crowded
conidiogenous loci, we prefer to keep it as a separate species.
Several authentic cultures and samples deposited by W.C. Snyder
have been examined, but unfortunately all of them date back to
1935 and 1940 and cannot be considered as type collections
since this species was already described in 1934. Therefore, a
neotypiication is necessary.
221
BenSch et al.
Records of C. pisicola from South Africa (Doidge et al. 1953,
Crous et al. 2000) are unclear and have to be proven and conirmed.
123. Cladosporium polygonati M.B. Ellis,
Dematiaceous Hyphomycetes: 338. 1976. Fig. 250.
More
Holotype: Ireland, Wicklow, Eniskerry, Bray, Powerscourt, on
Polygonatum sp. (Asparagaceae), Oct. 1965, C.H. Dickson (IMI
116694).
Lit.: David (1997: 57–58).
Ill.: David (1997: 38, ig. 8 D–G; 56, ig. 16).
In vivo: On faded leaves, distinct leaf spots lacking, with diffuse
discolorations or with dark spots, mainly caused by dense
fruiting. Colonies amphigenous, punctiform, brown, loose to
dense, scattered over the whole leaf surface. Mycelium internal.
Stromata lacking or only with small aggregations of a few swollen
hyphal cells. Conidiophores solitary to caespitose, erect, usually
strongly nodulose-geniculate, often almost monilioid, often swollen
at the conidiogenous cells, unbranched, 80–250(–300) × 5–16
μm, swellings up to 20 μm wide, basal cells also often swollen,
pluriseptate, often constricted at the septa, medium to dark brown
throughout, wall smooth to faintly rough-walled, wall thickened,
1–2 μm wide, one-layered or occasionally distinctly two-layered,
sometimes with a distict lumen giving the cells a seemingly
very thick-walled appearance. Conidiogenous cells integrated,
terminal and occasionally intercalary, 10–50 μm long, with 1–4
conspicuous conidiogenous loci, coronate, 3–4 μm wide and 1.5–2
μm high, somewhat darkened-refractive. Conidia solitary or in
short, unbranched chains, initially ovate with a swollen base, later
broadly ellipsoid-subcylindrical, 30–70 × 15–25 μm, 1–3-septate,
often constricted at the septa, olivaceous to pale brown, densely
echinulate, wall thin, 1–1.5 μm wide, ends rounded, with a
single conspicuous basal hilum or basal and terminal hila, barely
protuberant, 3–5 μm diam, somewhat darkened-refractive.
Substrate and distribution: On Polygonatum sp.; Ireland.
Notes: Cladosporium polygonati is easily distinguishable from all
other Cladosporium species due to very large, above all wide,
echinulate conidia, with distinct constrictions at the septa, and
strongly nodulose-nodose, often almost monilioid conidiophores.
The ecology of this species is not quite clear. This species is only
known from the type collection, which contains brown, nectrotic
leaves without distinct leaf spots, suggesting a saprobic fungus, but
it is also possible that this species initially occurs on green living host
leaves. New collections are necessary to answer these questions.
The type material of C. polygonati contains at least two additional
Cladosporium species, one with C. herbarum-like conidia, 8–20 ×
4–7(–8) μm, and another one with long, iliform-setiform, brown,
thick-walled conidiophores and small, pale, smooth to faintly roughwalled conidia, resembling C. tenuissimum.
124. Cladosporium populicola K. Schub. & U. Braun,
Schlechtendalia 14: 76. 2006. Figs 251, 252.
Holotype: Germany, Schleswig-Holstein, Missunde, Schlei ferry,
river bank, on Populus tremula (Salicaceae), 30 Aug. 2004, U.
Braun (HAL 1833 F).
Lit.: Schubert (2005b: 122–123).
Ill.: Schubert (2005b: 123, ig. 57, pl. 25, igs A–H), Schubert et al.
(2006: 77, ig. 10, pl. 2, igs D–F).
Fig. 250. Cladosporium polygonati (IMI 116694). Conidiophores and conidia in vivo.
Scale bar = 10 µm. U. Braun del.
222
In vivo: Leaf spots amphigenous, at irst small, subcircular to
irregular, later extending, becoming oblong or often irregular,
greyish white, surrounded by a distinct, narrow, irregular, dark brown
to almost blackish margin, inally conluent, covering large areas of
the leaves. Colonies amphigenous, loosely scattered, dark brown,
sometimes visible as greyish to blackish dots on the whitish spots.
Mycelium internal, subcuticular to intraepidermal; hyphae sparingly
branched, (2–)3–4.5 μm wide, septate, pale olivaceous-brown,
near the base of the conidiophores somewhat wider and darker,
with swellings and constrictions, smooth, with slightly to distinctly
thickened walls, occasionally even distinctly two-layered. Stromata
or stromatic hyphal aggregations absent to well-developed, mostly
small, 15–35(–45) μm diam, composed of swollen, subglobose
to somewhat angular-oblong cells, 6–11(–15) μm wide, medium
to dark olivaceous-brown, smooth, thick-walled. Conidiophores
solitary, in pairs or small loose groups, arising from swollen hyphal
cells or from stromata, erumpent through the cuticle, erect, straight
to slightly lexuous, subcylindrical or cylindrical-oblong, unbranched
to apically branched, not to sporadically somewhat geniculatesinuous, 50–175 × (4–)5–8(–11) μm, pluriseptate, not constricted
at the septa, medium to dark olivaceous-brown, somewhat
the genuS Cladosporium
clearly separated from the inner wall of the conidiophore, often
enteroblastically proliferating. Conidiogenous cells integrated,
terminal and intercalary, 6–24 μm long, proliferation sympodial,
with a single to several conidiogenous loci, sometimes located
on small lateral shoulders, protuberant, 1–2 μm diam, slightly
darkened-refractive. Conidia catenate, in unbranched or branched
chains, straight, obovoid, ellipsoid to rarely subcylindrical, 4–14
× 3–5(–5.5) μm, 0–1(–2)-septate, not constricted at the septa,
pale olivaceous to pale olivaceous-brown, smooth or almost so to
slightly verruculose (light microscopy), but most conidia minutely
verruculose when viewed by SEM, walls more or less thickened,
cells occasionally with a distinct lumen clearly separated from
the thick wall, apex rounded or slightly attenuated, with up to
three apical hila, protuberant, 1–2 μm diam, somewhat darkenedrefractive; microcyclic conidiogenesis not observed.
Substrate and distribution: On Populus tremula; Germany.
Fig. 251. Cladosporium populicola (HAL 1833 F). Symptoms, conidiophores and
conidia in vivo. Scale bar = 10 µm. K. Bensch del.
Notes: Several species of Cladosporium s. lat. have been described
on Populus spp. (C. asteroma, C. lethiferum, C. maculicola, C.
martianofianum, C. ramulosum, C. subsessile), but all of them
have to be excluded from Cladosporium s. str. and belong in
Fusicladium (Schubert et al. 2003). Type material of C. brunneum,
described on dead leaves of Populus sp., could not be traced at
PRM and is probably not preserved.
Cladosporium populicola is morphologically similar to C.
cladosporioides, which differs, however, in having narrower
conidiophores, 2.5–5 μm wide, with thinner and usually onelayered, not two-layered walls, without enteroblastic proliferations,
as well as longer conidia, 5–30 μm. Cladosporium oncobae is
also morphologically close to this species, but clearly separated
by narrower conidiophores, (2.5–)3–6(–7) μm wide, cells without a
distinct, separated lumen, and longer conidia, 3–20(–25) μm, with
up to three septa (Schubert et al. 2006).
125. Cladosporium praecox (Niessl)
Schlechtendalia 5: 34. 2000. Figs 253, 254.
U.
Braun,
Basionym: Fusicladium praecox Niessl, in Rabenhorst, Fungi Eur.
Exs., Ed. Nov., Ser. II, No. 1166. 1868 and Hedwigia 7: 124. 1868.
Lectotype (designated here): Czech Republic, “pr. Bistenz
ad Brunnam Moraviae”, on leaves of Tragopogon orientalis
(Asteraceae), May, G. de Niessl, Rabenhorst, Fungi Eur. Exs. 1166
(M-0057733). Isolectotypes: Rabenhorst, Fungi Eur. Exs. 1166
(e.g., B, HAL, HBG).
Lit.: Schubert (2005b: 124–125).
Ill.: Braun (2000: 33, ig. 3), Schubert (2005b: 124, ig. 58, Pl. 22,
igs G–I).
Exs.: Rabenhorst, Fungi Eur. Exs. 1166.
Fig. 252. Cladosporium populicola (HAL 1833 F). A. Symptoms. B. Overview. C, E.
Conidiophores with percurrent, enteroblastic proliferations and thickened, distinctly
two-layered walls. D. Conidiophore and conidia with coronate scars. F. Conidia
showing surface ornamentation. G. Tip of a conidiophore with young conidium. H.
Conidia. Scale bars = 10 (C–H), 20 (B) µm.
paler and attenuated towards the apex, smooth to occasionally
minutely verruculose, thick-walled, often distinctly two-layered,
(0.5–)1–2 μm wide, cells often with a distinct small inner lumen
www.studiesinmycology.org
In vivo: On living and fading leaves, causing diffuse yellowish
ochraceous to yellowish brown discolorations. Colonies
amphigenous, subeffuse, not very conspicuous, ochraceous,
brownish. Mycelium internal, substomatal and intraepidermal;
hyphae branched, 3–4.5 μm wide, septate, pale olivaceous,
smooth. Stromata absent to well-developed, 10–55 μm diam, cells
subcircular to irregular in outline, 2–8 μm diam, yellowish brown,
ochraceous. Conidiophores solitary or in small to moderately large
fascicles, loose to dense, arising from hyphae, swollen hyphal cells
223
BenSch et al.
Notes: Based on the cladosporioid structure of the conidiogenous
loci and hila Braun (2000) assigned Fusicladium praecox to
Cladosporium. Cladosporium praecox is well-characterised by
having uniformly short, very pale conidiophores. Cladosporium
agoseridis, known from North America on Agoseris spp., is easily
distinguishable in having longer and wider conidiophores and
conidia and, above all, wider conidiogenous loci and hila; and C.
inopinum, occurring on another host belonging to the Asteraceae,
differs in forming mainly external mycelium on trichomes and
0–3(–4)-septate conidia.
126. Cladosporium pseudiridis K. Schub., C.F. Hill, Crous
& U. Braun, Stud. Mycol. 58: 135. 2007. Figs 255, 256.
Fig. 253. Cladosporium praecox (M-0057733). Conidiophores and conidia in vivo.
Scale bar = 10 µm. K. Bensch del.
or stromata, emerging through stomata or erumpent through the
cuticle, erect, straight, subcylindrical to attenuated towards the apex,
somewhat geniculate-sinuous, unbranched or rarely branched,
8–54 × (2.5–)3.5–6(–7) μm, 0–1(–2)-septate, subhyaline to pale
yellowish ochraceous or yellowish brown, smooth, walls slightly
thickened, often somewhat swollen at the base, up to 9 μm wide.
Conidiogenous cells integrated, terminal, or conidiophores often
reduced to conidiogenous cells, 8–50 μm long, with one to several
conidiogenous loci, protuberant, (1–)1.5–2.5 μm diam, thickened,
darkened-refractive. Conidia catenate, in unbranched or branched
chains, ellipsoid-ovoid, fusiform, (6.5–)12–26(–31) × 4–7(–8) μm,
0–1(–3)-septate, yellowish, pale yellowish brown, ochraceous or
pale brown, faintly to conspicuously verruculose-echinulate, walls
thin to slightly thickened, with 1–3 hila, protuberant, 1–2 μm diam,
thickened, darkened-refractive; rarely microcyclic conidiogenesis
occurring.
Substrate and distribution: On Tragopogon orientalis; Czech
Republic.
Holotype: New Zealand, Auckland, Mt. Albert, Carrington Road,
Unitec Campus, isolated from lesions on living leaves Iris sp.
(Iridaceae), 15 Aug. 2004, C.F. Hill (CBS H-19861). Ex-type culture:
CBS 116463 = LYN 1065 = ICMP 15579.
Ill.: Schubert et al. (2007b: 136–137, igs 29–30).
In vitro: Mycelium sparingly branched, 2–7 μm wide, septate,
not constricted at the septa, subhyaline to pale brown, smooth
or almost so, walls somewhat thickened, guttulate or protoplasm
appearing granular, sometimes enveloped by a slime coat.
Conidiophores arising mostly terminally from ascending hyphae,
sometimes also laterally from plagiotropous hyphae, erect, more
or less straight, broadly cylindrical-oblong, once or several times
slightly to distinctly geniculate-sinuous, forming more or less
pronounced lateral shoulders, nodulose, unbranched, 100–320(–
500) × 7–11 μm, swellings 10–14 μm wide, becoming narrower and
paler towards the apex, septate, not constricted at the septa, septa
mainly basal, apical cell often very long, pale to medium olivaceousbrown, subhyaline at the apex, smooth or almost so, sometimes
minutely verruculose, walls usually distinctly thickened, sometimes
even two-layered, up to 1(–2) μm thick, protoplasm granular,
often clearly contrasting from the outer wall. Conidiogenous cells
integrated, terminal and intercalary, cylindrical-oblong, slightly
to distinctly geniculate-sinuous, nodulose with conidiogenous
loci conined to swellings or lateral shoulders, 30–110 μm long,
proliferation percurrent to sympodial, with a single or three,
sometimes up to ive geniculations per cell, usually only a single
Fig. 254. Cladosporium praecox (M-0057733). A. Fascicle of conidiophores. B. Conidiophores. C. Conidia. Scale bars = 10 (A–C) µm.
224
the genuS Cladosporium
Fig. 255. Cladosporium pseudiridis (CBS 116463). Conidiophores and conidia in
vitro. Scale bar = 10 µm. K. Bensch del.
locus per swelling, protuberant, very prominent, short cylindrical,
peg-like, clearly composed of a dome and surrounding rim, dome
often higher than the periclinal rim, broad, somewhat paler than
rim, conically narrowed, (2–)2.5–4 μm wide, up to 2 μm high,
thickened and darkened-refractive. Conidia solitary, sometimes in
short unbranched chains of two or three, straight to slightly curved,
young conidia small, 0–1-septate, broadly ovoid to pyriform, 15–26
× (9–)11–16(–18) μm [av. ± SD, 19.2 (± 4.3) × 14.2 (± 3) μm], irst
septum somewhat in the upper half, the upper cell is much smaller
but gradually extending as the conidium matures, mature conidia
1–3-septate, broadly pyriform, cylindrical-oblong or soleiform,
usually with a distinctly bulbous base, 30–55 × 12–19(–21) μm [av. ±
SD, 41.5 (± 6.8) × 17.1 (± 2.1) μm], broadest part of conidia usually
at the bulbous base, mostly attenuated towards the basal septum,
septa becoming sinuous with age, pale to medium olivaceousbrown or brown, usually echinulate, sometimes coarsely verrucose,
walls distinctly thickened, up to 2 μm thick, often appearing layered
with a large lumen in the centre of the cell, broadly rounded to
lattened at apex and base, hila often very prominent, often peglike elongated, up to 3 μm long, with age becoming less prominent,
visible as a thickened lat plate just below the outer echinulate wall
layer, slightly raised towards the middle, 2–3.5 μm diam, thickened
and darkened-refractive; microcyclic conidiogenesis not observed.
Culture characteristics: Colonies on PDA attaining 6 mm diam after
14 d at 25 ºC, whitish, smoke-grey to pale olivaceous-grey due
Fig. 256. Cladosporium pseudiridis (CBS 116463). A–C. Conidiophores and conidia. D. Part of a conidiogenous cell showing a protuberant cladosporioid conidiogenous locus.
E–F. Conidia. Scale bars = 10 µm.
www.studiesinmycology.org
225
BenSch et al.
to abundant aerial mycelium, olivaceous-black reverse, margin
narrow, white, more or less crenate, aerial mycelium zonate, luffy,
covering most of the colony, mainly in the colony centre, growth
convex to raised, deep into the agar, with age few large prominent
exudates formed, sparingly sporulating. Colonies on MEA attaining
7 mm diam after 14 d at 25 ºC, olivaceous-grey, pale olivaceousgrey to pale rosy-buff due to abundant aerial mycelium covering
almost the whole colony, iron-grey reverse, margin colourless or
white, broad, regular, more or less glabrous, aerial mycelium luffy,
dense, high, growth convex to umbonate, sometimes with elevated
colony centre, prominent exudates lacking, sporulation sparse.
Colonies on OA attaining 8 mm diam after 14 d at 25 ºC, white, pale
buff to pale olivaceous-grey in the centre, margin grey-olivaceous,
olivaceous- to iron-grey reverse, margin entire edge or somewhat
undulate, somewhat feathery, growth raised with a somewhat
depressed centre forming an elevated outer rim, without prominent
exudates, sporulation more abundant.
Substrate and distribution: On Iris sp.; New Zealand.
Notes: Host range and origin of C. pseudiridis, which is
morphologically close to the common and widespread C. iridis, are
still unknown as the genus Iris is not indigenous to New Zealand.
The latter species is easily distinguishable from C. pseudiridis by
its (0–)2–6(–7)-septate, longer and narrower conidia, (18–)30–75(–
87) × (7–)10–16(–18) μm (Schubert et al. 2007b).
127. Cladosporium pseudocladosporioides Bensch,
Crous & U. Braun, Stud. Mycol. 67: 71. 2010. Figs 257, 258.
Holotype: Netherlands, Zwolle, isol. from outside air, 7 Jan. 2007,
M. Meijer (CBS H-20445). Ex-type cultures: CBS 125993 = CPC
14189, CPC 14193
Ill.: Bensch et al. (2010: 71−72, igs 60−61).
Fig. 257. Cladosporium pseudocladosporioides (CBS 125993). Macro- and
micronematous conidiophores, ramoconidia and conidial chains in vitro. Scale bar
= 10 µm. K. Bensch del.
In vitro: Mycelium immersed and supericial; hyphae unbranched
or sparingly branched, (0.5−)1−4 μm wide, septate, sometimes
constricted at septa, especially in wider ones, subhyaline to pale
olivaceous or pale olivaceous-brown, smooth or almost so, walls
sometimes slightly thickened, about 0.5 μm wide, sometimes
irregular in outline due to swellings and constrictions, sometimes
forming small ropes of few hyphae, sometimes cells swollen,
up to 6.5 μm wide, fertile hyphae minutely verruculose, mainly
at the base of conidiophores. Conidiophores macronematous,
sometimes also micronematous, solitary or in small loose groups,
arising terminally and laterally from hyphae or swollen hyphal
cells, erect, straight to slightly lexuous, cylindrical-oblong, nonnodulose, sometimes once geniculate-sinuous or slightly swollen
at the apex, unbranched or branched once or twice, occasionally
three times, branches often only as short denticle-like lateral
outgrowth just below a septum, 15−155 μm long, 2−4 μm,
sometimes attenuated towards apex, 0−5-septate, sometimes
slightly constricted at septa, pale to pale medium olivaceousbrown, sometimes paler towards the apex, smooth or almost so,
at the base asperulate or inely verruculose like fertile hyphae,
walls slightly thickened, about 0.5 μm wide or unthickened;
micronematous conidiophores iliform, narrower, not attenuated,
about 1.8 μm wide. Conidiogenous cells integrated, terminal,
sometimes intercalary, slightly attenuated, narrowly cylindrical-
oblong, sometimes once geniculate, non-nodulose, (6.5−)9−33
μm long, with 1−4 loci at the apex, occasionally with up to seven
loci crowded at or towards the apex, in intercalary cells loci situated
on small lateral peg-like outgrowths, 1−2(−3) loci, conspicuous,
subdenticulate, 1−1.5(−1.8) μm diam, somewhat thickened and
darkened-refractive. Ramoconidia cylindrical-oblong, 19−48 ×
3−4 μm, 0−2(−3)-septate, pale olivaceous-brown, smooth, base
broadly truncate, 2−3 μm wide, unthickened or slightly thickened,
sometimes slightly refractive. Conidia very numerous, catenate, in
branched chains, branching in all directions with 3−6(−9) conidia
in the terminal unbranched part of the chain, small terminal conidia
obovoid, ovoid to limoniform or ellipsoid, sometimes subglobose,
3−5.5 × (1−)1.5−2.5 μm (av. ± SD: 4.1 ± 0.7 × 2.1 ± 0.3), apex
rounded or attenuated towards apex and base, intercalary conidia
ovoid, limoniform to ellipsoid or subcylindrical, 4.5−13(−19) ×
(1.8−)2−3 μm (av. ± SD: 8.8 ± 3.9 × 2.6 ± 0.3), 0(−1)-septate,
slightly attenuated towards apex and base, with 1−4(−5) distal
hila, secondary ramoconidia ellipsoid-ovoid to subcylindrical or
cylindrical-oblong, (6.5−)8−23(−29) × (2−)2.5−3.5(−4) μm (av. ±
SD: 16.1 ± 5.1 × 2.9 ± 0.3), 0−1(−2)-septate, septum median
or often somewhat in the lower half, pale olivaceous to pale
olivaceous-brown, smooth or almost so, sometimes slightly
rough-walled, walls unthickened, with (1−)2−4(−6) distal hila,
226
the genuS Cladosporium
Fig. 258. Cladosporium pseudocladosporioides (CBS 125993). A–F. Macronematous conidiophores and conidial chains. Scale bars = 10 µm.
conspicuous, subdenticulate, 0.5−1.5(−1.8) μm diam, somewhat
thickened and darkened-refractive; microcyclic conidiogenesis
not observed.
Culture characteristics: Colonies on PDA attaining 65−78 mm diam
after 14 d, olivaceous-grey to grey-olivaceous, reverse leaden-grey
to olivaceous-black, felty-loccose, margins regular, glabrous to
feathery, grey-olivaceous, aerial mycelium felty-loccose, growth
effuse to low convex, few small prominent exudates formed,
sporulation profuse. Colonies on MEA attaining 52−75 mm diam
after 14 d, smoke-grey to dark smoke-grey or grey-olivaceous,
reverse iron-grey, loccose, margins white, narrow, glabrous to
somewhat feathery, aerial mycelium white, loccose, abundant,
dense, growth effuse and somewhat radially furrowed, mostly
without prominent exudates, sporulation profuse. Colonies on OA
reaching 55−73 mm diam after 14 d, olivaceous to grey-olivaceous
or olivaceous-buff, pale olivaceous-grey to greenish grey towards
margins, reverse pale greenish grey, leaden-grey to iron-grey,
loccose, margins colourless, glabrous, regular, aerial mycelium
loccose to felty, sometimes covering large parts of colony surface,
growth effuse with few prominent exudates, sporulation profuse.
Substrates and distribution: On plant material and fungal fruiting
bodies, isolated from air, soil, water and food; widely distributed,
Africa (Uganda, South Africa), Asia (Indonesia, South Korea),
Australasia (Australia, New Zealand), Europe (France, Germany,
www.studiesinmycology.org
Italy, Netherlands, Romania, Russia, Slovenia), North America
(Canada, USA), South America (Brazil, Chile).
Additional specimens examined:Sine loco, isol. from cloud water, coll. & isol. by M.
Sancelme, ident. as C. tenuissimum by G.S. de Hoog (CBS 117134). Australia,
Bimbadeen Lookout, ca. 10 km of Cessnock, North Coast, isol. from Eucalyptus
placita (Myrtaceae), 10 Nov. 2006, coll. B.A. Summerell, isol. P.W. Crous, NSW
734672 (CPC 13683); New South Wales, Douglas Park, isol. from E. moluccana,
31 Aug. 2006, coll. B.A. Summerell, isol. P.W. Crous (CPC 13339, 13340). Brazil,
Vicosa, Parque National de Serra du Brigadeiro, isol. from Vernonia sp. (Asteraceae),
27 Jul. 2006, O. Pereira (CPC 13488). Canada, Ontario, Pearth, River Tay, isol. from
Sagittaria graminea (Alismataceae), 9 Jan. 2006, W. Gams & K.A. Seifert (CPC
13529); British Colombia, Victoria, isol. from Acer macrophyllum (Aceraceae), 9 Jun.
2007, coll. B. Callan, isol. P.W. Crous (CPC 14382). Chile, Easter Island, isol. from
soil, 2007, B. Andersen (BA 1694 = CPC 14295). France, Caves de Madelaine, isol.
from leaves, 21 Aug. 2007, P.W. Crous (CBS 126356 = CPC 14278). Germany,
Frankfurt/Main, Botanical Garden, isol. from Paeonia sp. (Paeoniaceae), 7 Oct.
2004, R. Kirschner [CBS 117153, stored as Graphiopsis chlorocephala (Fresen.)
Trail]; Schwäbische Alb, Kuppenalb, isol. from Myrothecium inundatum growing on
an old fungal fruit body, 5 Oct. 2006, M. Grube (CBS 126390 = CPC 13499, CPC
13500, 13501). Indonesia, Tele, isol. from Eucalyptus sp. (Myrtaceae), endophyte
spots after herbicide, 2008, coll. M.J. Wingield, isol. P.W. Crous (CPC 14992). Italy,
S. Michele all’Adige, isol. from leaves of Malus sylvestris (Rosaceae), depos. by
G. de Stanchina, Dec. 1980, ident. by G.S. de Hoog (CBS 667.80 = IHEM 3705).
Netherlands, Putten, isol. from pine needles of Pinus sp. (Pinaceae), 24 Jul. 2007,
P.W. Crous (CPC 14230). New Zealand, Auckland, Alfriston, Emblings Bridge,
-36.8667, 174.7667, isol. from leaves of Phalaris aquatica (Poaceae), 26 Nov. 2002,
C.F. Hill (Hill 730 = ICMP 14870 = CPC 11841). Romania, isol. from Pteridium
aquilinum (CBS 176.82). Russia, mycophilic, ident. by W. Gams as C. cladosporioides
(CBS 574.78A = VKM F-422); Moscow region, isol. from Melampsoridium betulae
(Pucciniastraceae), ident. by W. Gams as C. cladosporioides (CBS 574.78B = VKM
227
BenSch et al.
F-2759). Slovenia, Gabrovka, isol. from a fruit of Rosa canina (Rosaceae) attached
to shrub, 3 Jan. 2008, H.-J. Schroers (HJS 1038 = CPC 14975a). South Africa,
Eastern Cape, Aiwal North, isol. from wheat, 1989 (CPC 14020 = MRC 10814); Free
state, Amersfoort, isol. from oats, 1984 (CPC 14005 = MRC 03850); Danielsrus,
isol. from oats, 1983 (CPC 14003 = MRC 03366); Hoopstad, isol. from wheat, 1988
(CPC 14014 = MRC 10232); Modderpoort, Ladybrand, isol. from wheat, 1983 (CPC
14006 = MRC 03978); Slabberts, isol. from oats, 1983 (CPC 14001 = MRC 03240,
CPC 14002 = MRC 03245); Tugela, isol. from wheat, 1988 (CPC 14013 = MRC
10221); Westminster, isol. from oats, 1983 (CPC 14007 = MRC 03979); Northern
Cape, Prieska, isol. from Aloe dichotoma (Xanthorrhoeaceae), 2005 (CPC 13998 =
CAMS 001160); unknown location, isol. from Sorghum sp. (Poaceae), 1988 (CPC
14010 = MRC 10183, as C. cucumerinum). South Korea, Hoengseong, N37º32’09’’
E128º07’07’’, isol. from Agrimonia pilosa (Rosaceae), 4 Aug. 2004, H.-D. Shin
(CPC 11605); Namyangju, N37º34’59’’ E127º13’52’’, isol. from Glebionis coronaria
(= Chrysanthemum coronarium var. spatiosum) (Asteraceae), 30 Sep. 2004, H.D. Shin (CPC 11392). Uganda, Mubende, isol. from coffee leaf, 2000, coll. J.L.
Sørensen, isol. B. Andersen (BA 1677 = CPC 14357). USA, Illinois, Peoria, isol.
from Triticum aestivum (Poaceae), 1966, C.W. Hesseltine, NRRL A-14110 (CBS
H-10342, culture CBS 149.66); Louisiana, Baton Rouge, isol. from pruned wood,
2006, K.A. Seifert (CPC 12850); New York, Binghamton, isol. from creosote-treated
southern pine pole (ATCC 66669 = CPC 5100); Virginia, Front Royal, isol. from pods
of Kentucky coffee tree, 2007, P.W. Crous (CPC 13992).
Notes: Cladosporium pseudocladosporioides is a common,
widespread saprobic hyphomycete phylogenetically and
morphologically very close to C. cladosporioides but clearly
distinct by forming a separate lineage in phylogenetic analyses
(see Bensch et al. 2010, ig. 1, part b vs. c) and by having shorter
and somewhat narrower, 0−1(−2)-septate secondary ramoconidia,
narrower conidiogenous loci and hila, and hyphae sometimes
forming ropes. However, the distinction between the two species
only based on morphology is dificult and not always possible
with certainty, which is additionally complicated by the genetical
structure of the C. pseudocladosporioides clade suggesting that it
possibly represents a complex containing cryptic species (observed
in both ACT and TEF alignments in Bensch et al. 2010). Uncertain
strains should simply be referred to as C. cladosporioides s. lat.
(complex). Cladosporium paracladosporioides is also similar but
differs in having wider, 0−3-septate secondary ramoconidia, wider
conidiogenous loci and hila and is phylogenetically distinct (see
Bensch et al. 2010, ig. 1, part b).
Fig. 259. Cladosporium psoraleae (IMI 163005). Symptoms, conidiophores and
conidia in vivo. Scale bar = 10 µm. K. Bensch del.
128. Cladosporium psoraleae M.B. Ellis, Mycol. Pap. 131:
16. 1972. Figs 259, 260.
Holotype: Myanmar (Burma), Mandalay, on living leaves of Cullen
corylifolium (≡ Psoralea corylifolia) (Fabaceae), 25 Nov. 1971, M.
Thaung (IMI 163005).
Lit.: Ellis (1976: 344), Schubert (2005b: 125–126).
Ill.: Ellis (1972: 17, ig. 16; 1976: 343, ig. 260 C), Schubert (2005b:
126, ig. 59, pl. 26, igs A–D).
In vivo: Leaf spots amphigenous, subcircular to irregular in outline,
1–4(–9) mm wide, pale brown to greyish brown, surrounded
by a narrow, irregular, medium brown margin and a somewhat
irregular yellowish ochraceous halo. Colonies amphigenous,
short caespitose, pale greyish brown, short villose. Mycelium
internal, subcuticular; hyphae branched, 2–6 μm wide, septate,
not or slightly constricted at the septa, sometimes swollen, up to
8 μm wide, pale olivaceous, sometimes subhyaline, smooth, with
slightly thickened walls, forming swollen hyphal cells, subcircular
to somewhat oblong, up to 15 μm diam, smooth, walls thickened.
Conidiophores solitary or in loose, small groups, arising from
hyphae or swollen hyphal cells, erumpent through the cuticle,
228
Fig. 260. Cladosporium psoraleae (IMI 163005). A. Symptoms. B, C. Conidiophores.
D. Conidium. Scale bars = 10 (B–D) µm.
straight to slightly lexuous, somewhat geniculate-sinuous, nonnodulose, unbranched or only once or twice branched, 22–130
× 3–6 μm, septate, with 1–4 septa, not constricted at the septa,
pale brown, pale olivaceous, sometimes subhyaline, smooth,
walls slightly thickened, one-layered, occasionally swollen at the
base, up to 10 μm wide. Conidiogenous cells integrated, terminal
or intercalary, cylindrical, oblong, proliferation sympodial, with
numerous, occasionally crowded conidiogenous loci, protuberant,
sometimes subdenticulate, truncate to slightly convex, 1.5–3
μm diam, dome and periclinal rim conspicuous, thickened and
somewhat darkened-refractive. Conidia catenate, in unbranched or
branched chains, straight, cylindrical, ellipsoid, fusiform, limoniform,
5–30 × 3.5–7 μm, 0–1(–2)-septate, septum more or less median,
not constricted at the septum, subhyaline to pale brown, smooth,
the genuS Cladosporium
walls slightly thickened, apex rounded to attenuated or with 1–5
apical scars, truncate at the base, hila protuberant, truncate to
slightly convex, 1–2.5 μm diam, thickened, somewhat darkenedrefractive; microcyclic conidiogenesis not observed.
olivaceous-brown, smooth, somewhat attenuated towards apex and
base, with 3(–5) distal hila, protuberant, denticulate, 1–2 μm diam,
thickened and darkened-refractive; microcyclic conidiogenesis not
observed.
Substrate and distribution: On Cullen corylifolium, Myanmar.
Culture characteristics: Colonies on PDA reaching 10–27 μm diam
in 14 d at 25 °C, grey-olivaceous to olivaceous, becoming pale
olivaceous grey or smoke grey due to abundant aerial mycelium,
reverse olivaceous-grey to iron-grey and leaden-grey, velvety to feltywooly; margin narrow to wide, white, regular to undulate, glabrous
to feathery; aerial mycelium at irst absent, later abundantly formed,
felty, high; growth lat to later convex, sometimes either heaped
or radially furrowed; few prominent exudates formed; sporulation
profuse. Colonies on OA reaching 7–20 mm diam, at irst greyoliavceous to olivaceous, reverse leaden-grey to leaden-black,
later pale mouse-grey to pale olivaceous due to aerial mycelium,
reverse black, velvety to ilty; margin white, glabrous, regular or
either undulate or arachnoid, deeply furrowed; aerial mycelium
sparse to felty, dense, pale mouse-grey, covering only parts of the
colony, mainly the colony centre; growth lat with papillate surface;
without prominent exudates; sporulation profuse. Colonies on
MEA reaching 8–19 mm diam, grey-olivaceous, glaucous-grey at
margin, smoke-grey to pale mouse-grey or whitish due to aerial
mycelium, reverse olivaceous-grey to iron-grey, velvely to woolyfelty, margin white, narrow, glabrous to feathery, radially furrowed;
aerial mycelium abundant, luffy; few prominent exudates formed;
sporulation profuse. Colonies on MEA with 5 % NaCl growing much
faster than on other media, reaching 25–38 mm diam, of different
colours, mostly reseda-green and granulate due to profuse
sporulation, margin olive-yellow, reverse yellow to dark green.
Maximum tolerated salt concentration: MEA + 17 % NaCl after 14 d.
Cardinal temperatures: No growth at 4 °C, optimum and maximum
temperature at 25 °C (8–19 mm diam), no growth at 30 °C (from
Zalar et al. 2007).
Notes: Cladosporium psoraleae is morphologically close to C.
cladosporioides but distinct in having conidiogenous cells with
numerous, sometimes crowded conidiogenous loci, somewhat
wider conidia and, above all, conspicuous leaf spots. C. vignae is
also very close, but possesses somewhat narrower conidiogenous
loci and hila and somewhat shorter and narrower conidia;
subglobose and obovoid terminal conidia are usually lacking
in C. psoraleae. Owing to these small differences C. psoraleae
is tentatively maintained as a separate species, but additional
collections and, above all, molecular data are urgently needed to
clarify the status of this taxon. Bilgrami et al. (1991) cited a record
of the latter species on living leaves of Gardenia turgida from India
(UP), which seems to be very doubtful.
129. Cladosporium psychrotolerans Zalar, de Hoog &
Gunde-Cimerman, Stud. Mycol. 58: 175. 2007. Fig. 261.
Holotype: Slovenia, Sečovlje salterns, isolated from hypersaline
water, May 1999, S. Sonjak (CBS H-19730). Ex-type culture: EXF391 = CBS 119412.
Ill.: Zalar et al. (2007: 166, ig. 5 e, 176, ig. 11).
In vitro: Mycelium partly supericial and partly submerged, with
numerous lateral pegs, consistently enveloped in polysaccharidelike material; hyphae unbranched or sparingly branched, 1–3(–5)
μm wide, septate, not constricted at septa, pale brown or pale
olivaceous-brown, almost smooth to verruculose, thin-walled.
Conidiophores macronematous, arising terminally and laterally
from hyphae, erect or ascending, straight or somewhat lexuous,
neither geniculate nor nodulose, cylindrical-oblong, unbranched or
branched, once or few times, 20–220 × (2–)3–4(–5) μm, septate,
not constricted at septa, pale olivaceous-brown or brown, smooth
or almost so, sometimes verruculose at the base, walls slightly
thickenend, about 0.5 μm wide. Conidiogenous cells integrated,
terminal and intercalary, cylindrical, 12–65 μm long, producing
sympodial clusters of pronounced, conspicuous denticles (1–4 loci)
at their distal ends, loci 1.5–2 μm diam, often seceding at a septum
and behaving like conidia. Ramoconidia cylindrical with a broadly
truncate base, 19–43(–47) × (2–)3–4(–4.5) μm, aseptate, rarely
1(–2)-septate, not or only very slightly attenuated towards the
base, base 2–2.5 μm wide, somewhat darkened-refractive. Conidia
catenate, in branched chains, branching in all directions, terminal
chains with up to six conidia, small terminal conidia subglobose
to ovoid, globose, (2–)3–5 × 2–2.5(–3) μm (av. ± SD: 3.9 ± 0.8 ×
2.7 ± 0.4), aseptate, pale brown, smooth to minutely verruculose,
rounded at the apex, attenuated towards the base, hila 0.5–0.8
μm diam, intercalary conidia ovoid, limoniform to ellipsoid, 5–12
× 2.5–3(–3.5) μm (av. ± SD: 7.2 ± 1.9 × 3.2 ± 0.5), 0(–1)-septate,
pale brown, smooth to minutely verruculose, with up to three distal
hila, 0.5–1 μm diam, secondary ramoconidia ellipsoid to cylindrical,
(7.5–)12–25(–29) × 2.5–3.5(–4.5) μm (av. ± SD: 17.8 ± 5.6 × 3.3
± 0.4), 0–1(–2)-septate, not constricted at septa, pale brown or
www.studiesinmycology.org
Substrates and distribution: Isolated from hypersaline water, indoor
environments and plant material; Europe (Germany, Slovenia),
North America (USA), West Indies (Dominican Republic).
Additional specimens examined: Dominican Republic, salterns (EXF-714).
Germany, Sachsen-Anhalt, Halle, isol. from silicone at a window, 2007, U. Braun
(CPC 13822); botanical garden, isol. from leaves of Corylus avellana (Betulaceae),
11 Jun. 2004, K. Schubert (CPC 11330). Slovenia, Sečovlje salterns, isolated from
hypersaline water (EXF-326, EXF-332). USA, Seattle, University of Washington
campus, 47.6263, -122.3331, isol. from chasmothecia of Phyllactinia guttata
(Erysiphales) on leaves of Corylus avellana, 16 Sep. 2004, D. Glawe (CPC 11814,
CPC 11824, CPC 11834).
Notes: Cladosporium psychrotolerans, belonging to the C.
sphaerospermum complex, differs from C. halotolerans in having
0–1(–2)-septate secondary ramoconidia with septa neither
darkened nor thickened and globose, subglobose or ovoid small
terminal conidia. It seems to be more common than indicated in
Zalar et al. (2007) and also occur in indoor environments.
Cladosporium langeronii and C. psychrotolerans are closely
related but C. langeronii is particularly well distinguishable from
all other Cladosporium species by its slow growing colonies (1–7
mm diam / 14 d) and relatively large conidia (4–5.5 × 3–4 μm).
Cladosporium psychrotolerans has smaller conidia (3–4 × 2.5–3
μm) but a similar length : width ratio and faster expanding colonies
(8–18 mm diam / 14 d) (Zalar et al. 2007).
229
BenSch et al.
Fig. 261. Cladosporium psychrotolerans (CBS 119412) (from Zalar et al. 2007). A–D. Colony surface grown on PDA (A), OA (B), MEA (C) and MEA plus 5 % NaCl (D) of strains
incubated for 14 d at 25 ºC in darkness. E–F. Conidiophores. G. Apical part of a conidiophore. H–I. Secondary ramoconidia and conidia. E–I. All from 7-d-old SNA slide cultures.
All but C, from EXF-391 (ex-type strain); C, from EXF-714. Scale bars = 10 (G–I), 50 (F), 100 (E) µm.
130. Cladosporium ramotenellum K. Schub., Zalar, Crous
& U. Braun, Stud. Mycol. 58: 137. 2007. Figs 262–264.
Holotype: Slovenia, Sečovlje, isolated from hypersaline water from
reverse ponds, salterns, 2005, P. Zalar (CBS H-19862). Isotype: HAL
2026 F. Ex-type culture: CBS 121628 = CPC 12043 = EXF-454.
Ill.: Schubert et al. (2007b: 138–139, igs 31–33).
230
In vitro: Mycelium unbranched or only sparingly branched, 1.5–4
μm wide, septate, without swellings and constrictions, hyaline
or subhyaline, smooth, sometimes irregularly rough-walled,
walls unthickened. Conidiophores solitary, macronematous and
micronematous, arising as lateral branches of plagiotropous
hyphae or terminally from ascending hyphae, erect, straight or
slightly lexuous, cylindrical, neither geniculate nor nodulose,
without head-like swollen apices or intercalary swellings,
the genuS Cladosporium
0–1-septate, rarely up to 4-septate, subhyaline to very pale
olivaceous, smooth or almost so, with a broadly truncate base,
without any dome and raised rim, 2–3 μm wide, not thickened
but somewhat refractive. Conidia numerous, polymorphous,
catenate, in branched chains, straight, sometimes slightly curved,
small terminal conidia numerous, globose, subglobose or ovoid,
obovoid or limoniform, 2.5–7 × 2–4(–4.5) μm [av. ± SD, 5.1 (±
1.3) × 3.1 (± 0.6) μm], aseptate, without distal hilum or with a
single apical scar, intercalary conidia ellipsoid to subcylindrical,
8–15 × 3–4(–4.5) μm [av. ± SD, 11.5 (± 2.4) × 3.6 (± 0.5) μm],
0–1-septate; secondary ramoconidia subcylindrical to cylindricaloblong, 17–35 × 3–4(–5) μm [av. ± SD, 22.5 (± 5.6) × 3.7 (±
0.5) μm], 0–3-septate, not constricted at the septa, subhyaline
to very pale olivaceous, minutely verruculose (granulate under
SEM), walls unthickened or almost so, apex broadly rounded or
slightly attenuated towards apex and base, sometimes guttulate,
hila protuberant, conspicuous, 0.8–1.5(–2) μm diam, somewhat
thickened and darkened-refractive; microcyclic conidiogenesis
occurring.
Fig. 262. Cladosporium ramotenellum (CPC 12043). Conidiophores and conidia in
vitro. Scale bar = 10 µm. K. Bensch del.
unbranched, sometimes branched, branches often only as short
lateral prolongations, mainly formed below a septum, 14–110
× 2–4 μm, septate, not constricted at the septa, subhyaline to
pale olivaceous or brown, smooth to minutely verruculose, walls
unthickened, sometimes guttulate. Conidiogenous cells integrated,
terminal, sometimes also intercalary, cylindrical, not geniculate,
non-nodulose, 10–28(–50) μm long, proliferation sympodial, with
few conidiogenous loci, mostly 1–3, loci sometimes situated on
small lateral prolongations, protuberant, 0.5–1.5(–2) μm diam,
thickened and somewhat darkened-refractive. Ramoconidia
formed, cylindrical-oblong, up to 47 μm long, 2–4 μm wide,
Culture characteristics: Colonies on PDA reaching 46–49 mm
diam after 14 d at 25 ºC, olivaceous to grey-olivaceous due to
abundant sporulation, appearing zonate in forming concentric
zones, margin entire edge to slightly undulate, white, glabrous,
aerial mycelium absent or sparse, growth lat with a somewhat
folded and wrinkled colony centre, without prominent exudates,
sporulation profuse. Colonies on MEA reaching 48–49 mm diam
after 14 d at 25 ºC, grey-olivaceous to olivaceous-grey, velvety,
olivaceous-grey to iron-grey reverse, margin entire edge to
undulate, radially furrowed, colourless, glabrous to feathery,
aerial mycelium sparse, diffuse, growth lat with slightly elevated
colony centre, distinctly wrinkled, prominent exudates not
formed, abundantly sporulating. Colonies on OA attaining 40 mm
diam after 14 d at 25 ºC, grey-olivaceous, margin entire edge,
colourless or white, glabrous, aerial mycelium absent or sparse,
growth lat, without exudates, sporulation profuse.
Fig. 263. Cladosporium ramotenellum (CPC 12043). A, C. Macronematous conidiophore. B. Conidial chain. D. Micronematous conidiophore. E. Ramoconidia and conidia.
Scale bars = 10 µm.
www.studiesinmycology.org
231
BenSch et al.
Fig. 264. Cladosporium ramotenellum (CPC 12043). A. Survey of colony development showing a large bulbous “foot cell” that gives rise to conidiophores, which can be
branched. B. Details of conidiophores showing secondary ramoconidia and conidia. The inset shows scar formation on a conidiophore. C. Conidiophore and several conidia.
D. Details of ornamentation on conidia. Note the wide, but relatively low ornamentation units. E. A micrograph illustrating the organisation within a conidiophore. Scale bars =
5 (A–D), 10 (E) µm.
Substrate and distribution: Hypersaline water, air; Europe
(Slovenia).
Additional specimen examined: Slovenia, Ljubljana, isolated from an air conditioning
system (bathroom), 2004, M. Butala (CBS 121627 = CPC 12047 = EXF-967).
Notes: Due to its consistently minutely verruculose conidia, C.
ramotenellum was described in Schubert et al. (2007b) in a
revision of the C. herbarum complex, although this species, which
is probably a saprobe in air and hypersaline water, morphologically
rather resembles C. cladosporioides and C. tenellum. The latter
species is slower growing in vitro and has conidiophores with
numerous conidiogenous loci, usually crowded towards the apex
forming sympodial clusters of pronounced scars, and shorter,
somewhat wider, 0–1(–2)-septate conidia, 3–20(–28) × (2.5–)3–
5(–6) μm (Schubert et al. 2007b). Cladosporium cladosporioides is
easily distinguishable by its smooth conidia.
A collection of a Cladosporium on and in old, necrotic glumes and
utricles of Carex nigra, partly infected by Anthracoides heterospora
and associated with C. herbarum s. lat. (Poland, Bory Tucholskie
forest, Mętne Reserve, ca. 18 km NE of Chojnice, 11 Aug. 2005, M.
Piątek, HAL 2235 F), agrees well with the features of C. ramotenellum
(with ramoconidia, smooth or almost so, conidia and secondary
ramoconidia 4–30 × 2–6 μm, 0–4(–5)-septate, verruculose). However,
the conidiophores are longer, the conidiogenous cells are often
232
intercalary and the conidia are somewhat wider, up to 6 μm, with up
to 4(–5) septa. Cladosporium ramotenellum has been described from
cultures, i.e. its features and variability in vivo is unknown. Therefore,
the identiication of herbarium samples is rather dificult and almost
impossible without additional cultures.
Recent unpublished molecular studies indicate that C.
ramotenellum is a quite common saprobic species occurring on
various substrates with a wider geographic distribution than given
in Schubert et al. (2007b). Its species description will have to be
emended in a paper dealing with additions to the C. herbarum
complex.
131. Cladosporium rectangulare K. Schub. & U. Braun,
Sydowia 56(2): 309. 2004. Figs 265, 266.
Holotype: Ex England, intercepted at Hoboken, New Jersey, USA,
on still living and fading leaves of Prosthechea prismatocarpa
(≡ Epidendrum prismatocarpum) (Orchidaceae), 29 Sep. 1941,
D.P. Limber (BPI 427292, as “C. orchidearum”).
Lit.: Schubert (2005b: 127–128).
Ill.: Schubert & Braun (2004: 310, ig. 6), Schubert (2005b: 127, ig.
60, pl. 22, igs J–L).
the genuS Cladosporium
ramoconidia) ovoid, ellipsoid, subcylindrical to cylindrical, 5–33 ×
2.5–5(–6) μm, 0(–1)-septate, pale to medium olivaceous-brown
or somewhat reddish brown, almost smooth to verruculose, walls
only slightly thickened, apex rounded or slightly attenuated towards
apex and base, hila protuberant, 0.5–1.5 μm diam, somewhat
thickened and darkened-refractive; microcyclic conidiogenesis
often occurring with conidia forming secondary conidiophores and
conidia.
Substrate and distribution: On Prosthechea prismatocarpa;
England.
Notes: The characteristic features described above could also be
observed in a small dried culture added to this collection. There
are no differences between the collections in vitro and in vivo with
regard to colour, septation, ramiication and length, although these
features are often variable in cultures of Cladosporium species.
The distinctive right-angled branched and erect to decumbent,
geniculate-sinuous conidiophores segregate C. rectangulare from
the supericially similar C. cladosporioides and allied taxa.
132. Cladosporium rectoides Bensch, H.-D. Shin, Crous &
U. Braun, Stud. Mycol. 67: 73. 2010. Figs 267, 268.
Fig. 265. Cladosporium rectangulare (BPI 427292). Conidiophores and conidia in
vivo. Scale bar = 10 µm. K. Bensch del.
In vivo: On leaves, forming crustaceous, stromatic layers, effuse,
dark brown to black. Mycelium internal. Conidiophores solitary, erect
to decumbent, straight to lexuous, somewhat geniculate-sinuous,
occasionally subnodulose, at irst unbranched, later repeatedly
characteristically branched, right-angled, 25–125 × 2.5–4(–5) μm,
sometimes longer, attenuated towards the apex, septate, septa
not very conspicuous, olivaceous-brown to somewhat reddish
brown, paler towards the apex, smooth, walls slightly thickened,
occasionally two-layered, 0.5(–1) μm wide. Conidiogenous cells
integrated, terminal and intercalary, cylindrical, 8–28 μm long,
proliferation sympodial, with few subdenticulate, protuberant
conidiogenous loci, 0.5–1.5 μm diam, slightly thickened and
darkened-refractive. Conidia catenate, often in branched chains,
straight, small terminal conidia (without or only with a single
apical hilum) numerous, subglobose, ovoid, obovoid, limoniform,
ellipsoid, 2.5–9 × 2–4(–5) μm, aseptate, large conidia (secondary
Holotype: South Korea, Jinju, N35º11’24’’ E128º10’56’’, isol. from
Vitis lexuosa (Vitaceae), 18 Oct. 2004, coll. H.-D. Shin, isol. P.W.
Crous (CBS H-20446). Ex-type culture: CBS 125994 = CPC 11624.
Ill.: Bensch et al. (2010: 74−75, igs 62−63).
In vitro: Mycelium sparingly developed to more abundantly
supericial, hyphae unbranched or sparingly branched, 1−4.5 μm
wide, sometimes up to 6 μm wide, septate, not constricted at septa,
pale olivaceous or pale olivaceous-brown, smooth to minutely
verruculose or verruculose, sometimes verrucose, walls unthickened,
sometimes forming ropes. Conidiophores solitary, macronematous,
occasionally micronematous, arising terminally and laterally from
hyphae, erect, straight to slightly lexuous, narrowly cylindricaloblong, once or twice slightly to often distinctly geniculate-sinuous or
slightly nodulose but often neither geniculate nor nodulose, with few
apical loci, unbranched or branched, branches sometimes quite long,
up to 80 μm, conidiophores 19−210 × (2−)2.5−4 μm, pluriseptate,
not constricted at septa, sometimes growth proceeding at an angle
Fig. 266. Cladosporium rectangulare (BPI 427292). A. Right-angled branched conidiophore. B, C. Conidia. Scale bars = 10 (A–C) µm.
www.studiesinmycology.org
233
BenSch et al.
Fig. 267. Cladosporium rectoides (CBS 125994). Conidiophores, ramoconidia and
conidial chains in vitro. Scale bar = 10 µm. K. Bensch del.
of 45−90°, geniculations mostly intercalary, quite apart of the apex,
pale to pale medium olivaceous-brown, smooth, walls slightly thickwalled, about 0.5 μm wide. Conidiogenous cells integrated, terminal
and intercalary, distinctly sympodially proliferating, cylindrical-oblong,
sometimes geniculate or right-angled, 12−47 μm long, sometimes
few additional loci at a lower level, often arranged like a garland round
about the stalk, in intercalary cells loci often situated on small lateral
shoulders, conspicuous, subdenticulate, 1−2 μm diam, somewhat
thickened and darkened-refractive. Ramoconidia cylindrical-oblong,
16−56 × 3−4 μm, 0−1-septate, commonly formed, base truncate,
2.2−3 μm wide, not thickened, sometimes slightly refractive. Conidia
catenate, in branched chains, branching in all directions, up to 4(−5)
conidia in the unbranched terminal part of the chain, small terminal
conidia globose, subglobose to obovoid, (2.5−)3−4(−5) × 2−3 μm (av.
± SD: 3.8 ± 0.7 × 2.5 ± 0.4), aseptate, subhyaline, smooth, apex
rounded, the outer wall often seems to detach, somewhat refractive,
appearing to be like a halo and walls appearing to be thick-walled,
intercalary conidia ovoid, ellipsoid to subcylindrical, 5−15(−17) ×
(2.5−)3−3.5 μm (av. ± SD: 9.2 ± 3.2 × 3.0 ± 0.3), 0−1-septate, not
constricted at septum, with 1−3(−4) distal hila, the outer wall also
seems to detach as in small terminal conidia, secondary ramoconidia
ellipsoid to subcylindrical or cylindrical, 8−28 × (2.5−)3−4 μm (av.
Fig. 268. Cladosporium rectoides (CBS 125994). A–G. Macronematous conidiophores and conidial chains; small terminal conidia sometimes with surface ornamentation
indicated by the arrows in B. Scale bar = 10 µm.
234
the genuS Cladosporium
± SD: 17.4 ± 5.3 × 3.4 ± 0.3), 0−1(−2)-septate, pale olivaceousbrown, smooth, walls unthickened or slightly thick-walled, slightly
attenuated towards apex and base, cells with one or more cavities,
with 2−3(−4) distal hila, hila conspicuous, subdenticulate, 0.5−2 μm
diam, somewhat thickened and darkened-refractive; microcyclic
conidiogenesis not observed.
Culture characteristics: Colonies on PDA attaining 68−82 mm diam
after 14 d, olivaceous-grey to iron-grey, whitish to pale olivaceousgrey due to aerial mycelium, reverse olivaceous-black, velvety
to loccose or luffy, margins feathery, colourless, regular, aerial
mycelium mainly in colony centre, luffy-loccose, growth effuse,
deep into the agar, without prominent exudates, sporulation profuse.
Colonies on MEA reaching 65−80 mm diam after 14 d, smoke-grey,
grey-olivaceous to brownish, whitish to pale olivaceous-grey due
to aerial mycelium, reverse iron-grey, powdery to luffy-loccose,
margins regular, feathery, colourless, aerial mycelium mainly in
colony centre, loose, diffuse to densely luffy-loccose, growth
effuse, somewhat furrowed or wrinkled in colony centre, without
prominent exudates, sporulation profuse. Colonies on OA attaining
60−77 mm diam after 14 d, pale olivaceous-grey to smoke-grey,
greenish grey, iron-grey or grey-olivaceous at margins, reverse
sky-grey to olivaceous-grey, velvety to woolly-loccose, margins
colourless, glabrous, regular, aerial mycelium mainly in colony
centre, white to smoke-grey, loosely to densely loccose, growth
effuse, without prominent exudates, sporulation profuse.
Fig. 269. Cladosporium rhododendri (HAL 1834 F). Conidiophores and conidia in
vivo. Scale bar = 10 µm. K. Bensch del.
Substrate and distribution: On different plants; Asia (South Korea).
Additional specimen examined: South Korea, Jinju, N35º11’24’’ E128º10’56’’, isol.
from Plectranthus sp. (Lamiaceae), 1 Jul. 2004, coll. H.-D. Shin, isol. P.W. Crous
(CBS 126357 = CPC 11405).
Notes: Cladosporium rectoides resembles C. rectangulare, above
all with regard to its conidiophores, but the two species are
genetically quite distinct (see Bensch et al. 2010, ig. 1, part b vs. c),
and the latter species differs in lacking ramoconidia and in having
wider conidia [2−5(−6) μm wide] with secondary ramoconidia being
mostly aseptate and narrower conidiogenous loci and hila, 0.5−1.5
μm diam (Schubert & Braun 2004).
133. Cladosporium rhododendri K. Schub., Schlechtendalia
16: 69. 2007. Figs 269, 270.
Holotype: Germany, Sachsen-Anhalt, Halle (Saale), Pyrastraße,
on Rhododendron sp. (Ericaceae), 11 Jun. 2004, D. Seidel, mixed
infection with C. herbarum (HAL 1834 F).
Lit.: Schubert (2005b: 128–130).
Ill.: Schubert (2005b: 129, ig. 61, pl. 27, igs A–F), Braun &
Schubert (2007: 70, ig. 5).
In vivo: On living leaves, leaf spots amphigenous, irregular in shape
and variable in size, often arising at leaf margins and extending
towards the midrib, dark brown to somewhat greyish brown, on the
upper leaf surface often covered by the whitish grey, membranous
detached cuticle. Colonies hypophyllous, scattered, in dense, low
tufts, pale olivaceous, velvety. Mycelium internal, subcuticular to
intraepidermal. Stromata absent to well-developed, 20–50 μm diam,
composed of subglobose to somewhat oblong, densely aggregated
cells, 5–9 μm wide, pale to medium olivaceous-brown, smooth,
www.studiesinmycology.org
thick-walled. Conidiophores in small to large, loose to dense
fascicles, arising from swollen hyphal cells or stromata, usually
emerging through stomata, occasionally erumpent through the
cuticle, erect, straight to slightly lexuous, unbranched, occasionally
branched at the base, often somewhat geniculate-sinuous, without
intercalar swellings, 15–80 × 3–6 μm, mostly up to 50 μm long,
0–5-septate, not constricted at the septa, pale olivaceous to
medium olivaceous-brown throughout or sometimes slightly paler
towards the apex, smooth, walls slightly thickened, somewhat
swollen at the base and slightly attenuated towards the apex.
Conidiogenous cells integrated, terminal and intercalary, cylindrical,
8–30 μm long, proliferation sympodial, with several conidiogenous
loci, often crowded and situated on small shoulders, protuberant,
1–1.5(–2) μm diam, well differentiated in a central convex dome
and a periclinal rim, thickened, slightly darkened-refractive. Conidia
catenate, usually in branched chains, straight to slightly curved,
subglobose, obovoid, limoniform, ellipsoid, fusiform, subcylindrical,
1.5–18 × (1–)2–5 μm, 0–1(–2)-septate, septum more or less median
and not constricted, very pale olivaceous to pale olivaceous-brown,
smooth or almost so to minutely verruculose or irregularly roughwalled, walls only slightly thickened, apex rounded or somewhat
attenuated, hila protuberant, slightly convex, 0.5–1.5(–2) μm diam,
dome and raised rim conspicuous, thickened, slightly darkenedrefractive; occasionally with microcyclic conidiogenesis.
Substrate and distribution: On Rhododendron sp.; Germany.
Notes: The hyphomycete found on a herbarium sample deposited
under the name “Cladosporium rhododendri” (in herb., on
Rhododendron sp., Switzerland, Bern, Berner Oberland, vom
Faulhorn, 7/58, B 700006700, without any author and collector)
does not belong in Cladosporium s. str. and must be excluded,
although the generic afinity of this fungus is not yet clear.
235
BenSch et al.
Fig. 270. Cladosporium rhododendri (HAL 1834 F). A. Fascicle of conidiophores. B. Conidial chains. C. Tip of a conidiophore with still attached conidium. D. Conidial chain,
conidia just separating but still attached at the central domes. E, F. Fascicles of conidiophores emerging through stomata and conidia. Scale bars = 10 (B–F), 50 (A) µm.
Cladosporium rhododendri pertains to a group of leafspotting Cladosporium species characterised by relatively short,
fasciculate conidiophores usually emerging through stomata, as
e.g., C. praecox [conidia larger, above all wider, (6.5–)12–26(–
31) × 4–8 μm, faintly to conspicuously verruculose-echinulate],
C. orchidearum (conidia wider, 4–7 μm, 0–3-septate, usually
verruculose, and loci wider, 1.5–2.5 μm diam), C. agoseridis
[conidiophores wider, (3–)4–10(–13) μm, conidia larger and,
above all, wider, (10–)12–40(–50) × (5–)6–13(–15) μm, densely
verrucose] and C. lupiniphilum (conidiophores wider, 3–8 μm,
conidia larger and, above all, wider, 6–28 × 4–8 μm) [Braun 1998,
2000, Braun & Rogerson 1995, Ellis 1976, Schubert & Braun 2004].
It is morphologically also close to C. jacarandicola, described
236
from New Zealand on living leaves of Jacaranda mimosifolia,
which is, however, distinguished by having 0–3-septate conidia
(Schubert & Braun 2004).
134. Cladosporium rivinae Speg., Anales Mus. Nac.
Buenos Aires 20: 437. 1910. Figs 271, 272.
Holotype: Argentina, near Metán, Salta, on living leaves of Rivina
humilis (= R. laevis) (Phytolaccaceae), Jun. 1905, C. Spegazzini,
Mycetes Argent. Nr. 1108 (LPS 13.137).
the genuS Cladosporium
Fig. 271. Cladosporium rivinae (LPS 13.137). Symptoms, conidiophores and conidia
in vivo. Scale bar = 10 µm. K. Bensch del.
Lit.: Saccardo (1913a: 1369), Farr (1973: 251), Schubert (2005b:
130–131).
Ill.: Schubert (2005b: 131, ig. 62, pl. 26, igs E–H).
In vivo: Leaf spots amphigenous, subcircular-oval to somewhat
irregular, 2–7 mm wide, very pale yellowish ochraceous,
membranous, pale greyish brown in the centre, sometimes
surrounded by a narrow, pale yellowish brown, raised margin,
inally leaf spots turning fragile and dropping out, forming shot
hole symptoms. Colonies epiphyllous, loose, pale greyish brown,
villose, often only sparingly fruiting. Mycelium internal; hyphae
branched, 1.5–7 μm wide, septate, often with small swellings and
constrictions, subhyaline to pale olivaceous or pale olivaceousbrown, smooth, walls unthickened or slightly thickened, at the base
of the conidiophores somewhat swollen and darker, concolorous
with conidiophores, up to 10 μm wide, sometimes aggregated.
Stromata absent. Conidiophores solitary or in small groups of
2–3, non-fasciculate, arising from hyphae or swollen hyphal cells,
erumpent through the cuticle, sometimes also emerging through
stomata, erect, more or less straight, sometimes slightly lexuous
towards the apex, iliform, attenuated towards the apex, usually
non-geniculate and non-nodulose, unbranched, rarely branched at
the apex, 40–240 μm long, (4–)6–9(–11) μm wide near the base,
narrower towards the apex, 3.5–5 μm wide, septate, sometimes
Fig. 272. Cladosporium rivinae (LPS 13.137). A. Symptoms. B. Bulbous, distinctly swollen bases of conidiophores. C. Tip of a conidiophore with several darkened-refractive
conidiogenous loci. D. Conidia. Scale bars = 10 (B–D) µm.
www.studiesinmycology.org
237
BenSch et al.
slightly constricted at the septa, pale to medium olivaceous-brown,
smooth to somewhat asperulate with age, walls thickened, mostly
distinctly two-layered, 0.5–2(–2.5) μm wide, usually distinctly
swollen, bulbous and somewhat darker at the base, up to 16 μm
wide. Conidiogenous cells integrated, terminal and intercalary,
6–26 μm long, proliferation sympodial, with a single to several
conidiogenous loci, often somewhat crowded near the apex,
protuberant, subdenticulate, 1–2 μm diam, thickened, darkenedrefractive. Conidia catenate, in branched chains, more or less
straight, globose or almost so, obovoid, ellipsoid to cylindrical, 3–23
× 2.5–4.5(–6) μm, 0–3-septate, sometimes slightly constricted at
the septa, very pale olivaceous to pale olivaceous-brown, almost
smooth to usually minutely verruculose, apex rounded or with a
single or several hila, protuberant, short cylindrical, 0.5–2 μm diam,
thickened, darkened-refractive; microcyclic conidiogenesis not
observed.
Substrate and distribution: On Rivina humilis; Argentina.
Notes: Cladosporium rivinae, which is the only biotrophic species
occurring on a host belonging to the Phytolaccaceae, differs from
all morphologically allied taxa in having conidiophores with a wide,
distinctly swollen, bulbous base, up to 16 μm wide (Schubert
2005b). Recently, C. basiinlatum has been described in vitro which
also possesess conidiophores being distinctly attenuated towards
the apex and having swollen, but often foot-like basal cells. Isolated
from Hordeum in Germany, this specis differs from C. rivinae in
having usually aseptate conidia and rigid conidiophores with only
terminal conidiogenous cells (Bensch et al. 2010).
135. Cladosporium robiniae (Kabát & Bubák) J.C. David,
Mycol. Pap. 172: 92. 1997. Fig. 273.
Basionym: Heterosporium robiniae Kabát & Bubák, Hedwigia 43:
421. 1904.
Lectotype (designated here): Czech Republic, Bohemia, Turnau
[Turnov], on Robinia pseudoacacia (Fabaceae), 20 Nov. 1904, J. E.
Kabát (BPI 802193). Isolectotypes: Kabát & Bubák, Fungi Imperf.
Exs. 596 (e.g., BPI 802192, K).
Lit.: Saccardo (1906: 585), Lindau (1910: 84), David (1997: 92–94).
Ill.: David (1997: 84, ig. 19 H–K, 94, ig. 24).
Exs.: Kabát & Bubák, Fungi Imperf. Exs. 596.
In vivo: Leaf spots lacking or only with discolorations, up to 1.5
mm diam, subcircular to angular, solitary to conluent. Caespituli
amphigenous, mainly hypophyllous, punctiform, brown, loose to
dense. Mycelium internal. Stromata lacking or sparingly developed
as loose stromatic aggregations of swollen hyphal cells at the
base of the conidiophore tufts. Conidiophores in well developed
fascicles, about 20 to 30, loose to moderately dense, arising from a
stromatic base, erumpent, erect, straight, subcylindrical, but usually
moderately to strongly geniculate-sinuous or geniculate-nodulose,
the swellings being well spaced, unbranched, 100–250 μm long,
5–7.5(–8.5) μm wide, up to 10 μm wide at nodes, pluriseptate
throughout, pale to medium brown or reddish brown, paler to wards
the tips, wall thin or somewhat thickened, 0.5–1.5 μm wide, cells
sometimes with distinct lumen (then cells of the conidiophores
seemingly very thick-walled, at the septa up to 4 μm), wall smooth
or occasionally slightly roughened. Conidiogenous cells integrated,
238
Fig. 273. Cladosporium robiniae (BPI 802193). Conidiophores and dimorphic
conidia in vivo. Scale bar = 10 µm. U. Braun del.
terminal and intercalary, 10–40 μm long, with a single or several
conidiogenous loci, distinctly coronate, somewhat protuberant,
thickened and pigmented, 1.5–2(–2.5) μm diam. Conidia dimorphic,
concolorous with the conidiophores, small conidia solitary or in
short, usually simple chains, ellipsoid-ovoid to cylindrical, 10–25
× 5–8.5 μm, 0–3(–4)-septate, verrucose, large conidia solitary,
broadly ellipsoid-ovoid to subcylindrical, 22–45 × 8.5–18 μm,
0–4(–6)-septate, coarsely verrucose-echinulate, wall 0.5–1.5 μm
wide, apex broadly rounded, base rounded to sligthly attenutaed,
hila coronate, somewhat thickened and darkened, 1–2.5 μm diam;
microcyclic conidiogenesis not observed.
Substrate and distribution: On Robinia pseudoacacia; Europe
(Czech Republic), North America (USA, NY).
Additional specimen examined: USA, New York, Ithaca, Cornell University Campus,
on Robinia pseudacacia, 2 Nov. 1939, J.É. Jacques 84 (CUP 26875).
Notes: Cladosporium robiniae is a Heterosporium-like species,
easily distinguishable from allied species by its fasciculate,
nodulose conidiophores and dimorphic conidia.
David (1997) considered BPI material to be the holotype, which
is, however, incorrect. Kabát and Bubák distributed duplicates of
the type material as Fungi Imperf. Exs. 596, but did not designate
holotype material. Therefore, the BPI material concerned should
rather be used for a lectotypiication.
the genuS Cladosporium
loci, protuberant, 1.5–2.5 μm diam, thickened and darkened.
Conidia catenate, often in branched chains, broadly ellipsoid-ovoid,
subcylindrical-fusiform, 6–18 × 3–6 μm, 0–1-septate, subhyaline
to pale olivaceous, almost smooth to verruculose, ends obtuse
to attenuated, hila protuberant, 1.5–2.5 μm diam; thickened and
darkened.
Substrate and distribution: On Ruta graveolens; Azerbaijan.
Notes: During the course of monographic studies within the
genus Ramularia, Braun (1998) re-examined the type material
of Ramularia rutae. Due to the cladosporioid structure of the
conidiogenous loci and hila and the pigmented conidiophores he
placed it in Cladosporium s. str. stating that there is no closely allied
species in the latter genus.
137. Cladosporium salinae Zalar, de Hoog & GundeCimerman, Stud. Mycol. 58: 175. 2007. Fig. 275.
Holotype: Slovenia, Sečovlje salterns, isolated from hypersaline
water, Feb. 1999, S. Sonjak (CBS H-19731). Ex-type culture: CBS
119413 = EXF-335.
Ill.: Zalar et al. (2007: 166, ig. 5 f, 177, ig. 11).
Fig. 274. Cladosporium rutae (BAK). Conidiophores loosely fasciculate,
conidiogenous cells and conidia in vivo. Scale bar = 10 µm. U. Braun del.
136. Cladosporium rutae (T.M. Akhundov) U. Braun, A
monograph of Cercosporella, Ramularia and allied genera
(phytopathogenic hyphomycetes), Vol. 2: 306. 1998. Fig.
274.
Basionym: Ramularia rutae T.M. Akhundov, Novosti Sist. Nizsh.
Rast. 24: 96. 1987.
Holotype: Azerbaijan, Apsheron, botanical garden, on Ruta
graveolens (Rutaceae), 25 Mar. 1961, Akhundov (BAK). Isotype:
LE 42008.
Lit.: Schubert (2005b: 132).
Ill.: Braun (1998: 307, ig. 571), Schubert (2005b: 132, ig. 63).
In vivo: Leaf spot amphigenous, shape and size variable, often
entire leaves discoloured, yellowish, ochraceous to pale brown,
margin indeinite. Colonies amphigenous, mostly hypophyllous,
effuse to dense, greyish white to pale brown. Mycelium internal;
hyphae pale, forming loose to dense hyphal aggregations,
substomatal to intraepidermal, pale olivaceous to olivaceous-brown,
composed of swollen hyphal cells, 3–8 μm diam. Conidiophores
solitary to caespitose, loosely fasciculate, arising from stromatic
hyphal aggregations, erect, straight, subcylindrical to somewhat
geniculate-sinuous, often somewhat attenuated towards the apex,
unbranched, rarely branched, 10–40 × 3–7 μm, 0–1(–2)-septate,
subhyaline to pale olivaceous or olivaceous-brown, smooth.
Conidiogenous cells integrated, terminal or conidiophores often
reduced to conidiogenous cells, with a single or two conidiogenous
www.studiesinmycology.org
In vitro: Mycelium partly supericial partly submerged, sparingly
developed, 1–3 μm wide, with numerous lateral pegs but not
distinctly branched, septate, not constricted at septa, subhyaline
to pale brown, smooth or almost so to sometimes irregularly roughwalled, walls unthickened or somewhat thickened, consistently
enveloped in polysaccharide-like material. Conidiophores poorly
differentiated, micronematous, arising terminally and laterally from
hyphae, erect, straight to lexuous, iliform to narrowly cylindricaloblong, slightly to distinctly geniculate-sinuous at or towards
the apex, once or several times and often in short succession,
sometimes bent, non-nodulose, unbranched or occasionally
branched with short peg-like lateral outgrowths, 5–60(–75) × (1.5–)
2–3(–4) μm, irregularly densely septate with 0–4(–6)-septa, not
constricted at septa, pale to medium olivaceous-brown or brown,
smooth to often minutely verruculose or irregularly rough-walled,
walls somewhat thickenend. Conidiogenous cells integrated,
mostly terminal, sometimes intercalary, narrowly cylindrical, slightly
to distinctly geniculate-sinuous towards the apex forming sympodial
clusters of pronounced denticles at their distal end, loosely to
densely crowded, conidiogenous loci protuberant, 1–1.5 μm diam,
thickened and darkened-refractive. Conidia catenate, in branched
chains, branching in all directions, terminal chains with up to six
conidia, straight, small terminal conidia obovoid, 3–6 × 2–3 μm [av.
(± SD) 4.8 (± 1.2) × 2.4 (± 0.4)], aseptate, smooth to occasionally
minutely verruculose, hila 0.8–1 μm diam, intercalary conidia with
1–2 distal hila fusiform or ellipsoid, 6–9(–10) × (2–)2.5–3(–3.5) μm
[av. (± SD) 7.5 (± 1.1) × 3.0 (± 0.4)], 0(–1)-septate, usually smooth,
sometimes very slightly rough-walled, attenuated towards apex
and base, hila 0.8–1 μm diam, secondary ramoconidia ellipsoid to
cylindrical, (7.5–)8–19(–25) × 2.5–3.5(–4.5) μm [av. (± SD) 13.4 (±
4.0) × 3.1 (± 0.5)], 0–1(–3)-septate, not constricted at septa, septa
becoming sinuous with age, pale to pale medium olivaceous-brown
or brown, smooth or almost so, sometimes very slightly roughwalled, walls thickened, slightly attenuated towards apex and
base, with up to six pronounced denticulate distal hila, sometimes
239
BenSch et al.
Fig. 275. Cladosporium salinae (CBS 119413). A–D. Colony surface grown on PDA (A), OA (B), MEA (C) and MEA plus 5 % NaCl (D) of strains incubated for 14 d at 25 ºC in
darkness. E–F. Habit of conidiophores. G. Conidiophore. H. Detail of apical part of conidiophore. I. Conidia. J. Secondary ramoconidia and conidia. E–J. All from 7-d-old SNA
slide cultures. A–D, from EXF-604; E–J, from EXF-335 (ex-type strain). Scale bars = 10 (H–J), 30 (G), 50 (F), 100 (E).
situated on small irregular lateral outgrowth, hila 1–1.5(–1.8)
μm diam, thickened and darkened; microcyclic conidiogenesis
occasionally occurring.
Culture characteristics: Colonies on PDA reaching 10–27 mm
diam, greenish olivaceous to grey-olivaceous or dull-green,
reverse leaden-grey, felty-wooly or velvety, margin narrow, white,
feathery, regular to undulate, aerial mycelium formed, felty-wooly,
high, growth low convex, without prominent exudates, sporulation
profuse. Colonies on OA reaching 7–20 mm diam, grey-olivaceous
240
to dull-green or mouse-grey, reverse olivaceous-grey to iron-grey
or dark mouse-grey, velvety, aerial mycelium sparse, smoke-grey,
diffuse to luffy, growth lat with papillate surface, without prominent
exudates, sporulation profuse. Colonies on MEA reaching 8–19
mm diam, grey- to greenish olivaceous, reverse olivaceous-grey
to iron-grey, velvety to luffy-felty, margin white, narrow, feathery to
undulate, aerial mycelium mainly in colony centre, felty-luffy, high,
colonies radially furrowed, wrinkled or folded, centre elevated,
without prominent exudates, sporulation profuse. Colonies on MEA
with 5 % NaCl growing much faster than on other media, reaching
the genuS Cladosporium
25–38 mm diam, of different colours, mostly reseda-green (2E6)
and granulate due to profuse sporulation, margin olive-yellow
(2D6), reverse yellow to dark green. Maximum tolerated salt
concentration: MEA + 17 % NaCl after 14 d. Cardinal temperatures:
No growth at 4 °C, optimum and maximum temperature at 25 °C
(8–19 mm diam), no growth at 30 °C.
Substrate and distribution: Isolated from hypersaline water in the
Mediterranean basin; Europe (Slovenia, Spain).
Additional specimens examined: Slovenia, Sečovlje salterns, isol. from hypersaline
water (EXF-322). Spain, Santa Pola, isol. from hypersaline water (EXF-604).
Notes: Cladosporium salinae morphologically resembles species
of the genus Fusicladium because its conidia are oblong-ellipsoid
to fusiform and conidiogenous loci of ramoconidia are placed
closely together. As any other Cladosporium species, its conidia
show typical cladosporioid scar structures, however. Cladosporium
salinae seems to have a separate position within the genus
Cladosporium since it seems to be distantly related to any other
described Cladosporium species or currently known species
complex within Cladosporium (Zalar et al. 2007).
138. Cladosporium sarmentorum (Riedl & Ershad) U.
Braun, Schlechtendalia 19: 63. 2009. Fig. 276.
Basionym: Acrosporella sarmentorum Riedl & Ershad, Sydowia 29:
166. 1977.
Holotype: Iran, village Love, near Minoudasht, 100–200 m high,
on dead tendrils of Rubus sp. (Rosaceae), 23 Apr. 1974, Riedl &
Ershad F5 (W). Isotype: EVIN.
Ill.: Riedl & Ershad (1977: 167, ig. 4), Braun (2009: 64, ig. 1).
In vivo: Colonies on dead stems, wood and tendrils, punctiform,
scattered to aggregated, or forming dense villose black patches
or layer. Mycelium internal, sometimes also external; hyphae pale
olivaceous to medium brown or olivaceous brown, 2–7 μm diam,
septate, smooth. Stromata lacking to well-developed, immersed
to erumpent, 20–120 μm diam, dark brown, composed of swollen
hyphal, 3–15 μm diam, brown, wall up to 1.5 μm thick, often with
diffuse aggregations and layers of stromatic hyphal cells, often
forming supericial strands of swollen cells, up to 10 μm diam,
with constrictions at the septa, monilioid. Conidiophores solitary,
caespitose, in small to large, loose to moderately dense fascicles,
arising from swollen hyphal cells or stromata, erect to decumbent,
20–250 × 3–9 μm, simple to frequently branched in the upper
portion, with short lateral branchlets or long branches, up to 90
μm in length, subcylindrical to sinuous, slightly geniculate, rarely
subnodulose, pluriseptate throughout, often densely septate and
constricted at the septa, pale to dark brown or olivaceous-brown,
tips usually paler, subhyaline to olivaceous, wall 0.5–1(–2) μm thick,
smooth to somewhat rough-walled or rugose, cells sometimes
with distinct lumina, giving the impression that the walls are very
thick, occasioanlly guttulate, rarely with monopodial rejuvenation.
Conidiogenous cells integrated, terminal and intercalary, 5–25 μm
long, at irst often monoblastic, later polyblastic, sympodial, but
conidiogenous cells not or only slightly geniculate, conidiogenous
loci (1–)1.5–2(–2.5) μm diam, less conspicuous than in most
other species of the genus, barely or only slightly protuberant,
slightly darkened-refractive, central dome not very conspicuous.
www.studiesinmycology.org
Fig. 276. Cladosporium sarmentorum (W). Fascicle of conidiophores arising
from stromata, frequently branched upper portions of conidiophores, conidia and
microcyclic conidiogenesis in vivo. Scale bar = 10 µm. U. Braun del.
Conidia solitary and catenate, in short simple to branched chains,
subglobose, broadly ellipsoid-ovoid, fusiform, subcylindrical,
limoniform, (3–)5–24(–30) × (2–)4–7(–8) μm, 0–3(–5)-septate, often
slightly constricted at the septa, small, young conidia subhyaline,
later olivaceous, olivaceous-brown to medium brown, wall up to
1 μm wide, smooth, rarely faintly rough-walled, ends rounded to
somewhat attenuated, hila not or somewhat protuberant, 0.75–2
μm diam, slightly darkened-refractive; occasionally with microcyclic
conidiogenesis.
Substrate and distribution: Saprobic on dead wood, stems and
tendrils; Asia (Iran), Europe (Italy, Russia).
Additional specimens examined: Italy, South Tirol, Trentino, Seis, on dead wood
(planks), 1870, G. Hausmann (B 700006723). Russia, Leningrad Oblast, Vyborg
District, Roshchino, Pionerskoje Forestry, on dead wood of Picea abies (Pinaceae),
23 Aug. 2005, D.A. Shabunin (HAL 2172 F, LE 256895); St. Petersburg, Vyborg
District, Institutskij prospect, near house 21, on dead cones of Picea pungens, 24
Dec. 2009, D.A. Shabunin (HAL 2350 F).
Notes: This is a saprobic species. The colonies, conidiophores
and conidia resemble those of C. delicatulum and C. nigrellum,
but the conidiophores are frequently branched. Cladosporium
sarmentorum differs from most other Cladosporium species in
having barely geniculate conidiogenous cells with less conspicuous,
barely protuberant, barely or only slightly darkened-refractive
conidiogenous loci. The loci are cladosporioid, but the central dome
is not very conspicuous and the surrounding rim is rather low.
241
BenSch et al.
Cladosporium xylophilum, another species occurring on wood, is
easily distinguishable from the latter species in having narrower,
usually unbranched conidiophores, 2–4 μm wide, and narrower
[(2−)2.5−4(−5) μm wide], 0–1(–3)-septate, smooth or often
irregularly rough-walled, loosely verruculose to verrucose conidia
with numerous distal hila crowded at the apex or situated on small
lateral prolongations (Bensch et al. 2010).
139. Cladosporium scabrellum Bensch, Schroers, Crous &
U. Braun, Stud. Mycol. 67: 75. 2010. Figs 277–279.
Holotype: Slovenia, Boštanj near Sevnica, on wilted part of leaf of
Ruscus hypoglossum (Asparagaceae), 2 Jan. 2008, H.-J. Schroers
(CBS H-20447). Ex-type culture: CBS 126358 = CPC 14976 = HJS
1031.
Ill.: Bensch et al. (2010: 76−77, igs 64−66).
In vitro: Mycelium immersed and supericial, hyphae unbranched
or loosely branched, 0.5−4 μm wide, mostly 1−3 μm wide, septate,
sometimes constricted at septa, subhyaline to pale medium
olivaceous-brown, smooth to minutely verruculose or loosely
verruculose, walls unthickened or almost so, often with intercalary
swellings and constrictions, forming loose to usually dense long
characteristic ropes, somewhat interlaced, hyphae which give rise
Fig. 277. Cladosporium scabrellum (CBS 126358). Macro- and micronematous
conidiophores, ramoconidia and conidial chains in vitro. Scale bar = 10 µm. K.
Bensch del.
Fig. 278. Cladosporium scabrellum (CBS 126358). A–D (after 3 d). Macronematous conidiophores arising solitary or in small loose groups from hyphae or swollen hyphal cells
and short conidial chains. E–H (after 7 d). Macronematous conidiophores and conidial chains. Scale bar = 10 µm.
242
the genuS Cladosporium
obovoid, 3.5−4.5(−5) × 2−2.2(−2.5) μm (av. ± SD: 4.1 ± 0.5 × 2.1
± 0.2), aseptate, intercalary conidia limoniform, fusiform, ellipsoid
to subcylindrical, 5−13 × 2−3(−3.5) μm (av. ± SD: 8.2 ± 2.9 × 2.7 ±
0.4), 0−1-septate, not constricted, slightly attenuated towards apex
and base, with 1−4 distal hila, secondary ramoconidia ellipsoid to
subcylindrical or cylindrical, 10−25 × 2.5−3.5(−4) μm (av. ± SD:
17.2 ± 4.6 × 3.1 ± 0.4), 0−1-septate, not constricted, with 3−4(−5)
distal hila, pale to pale medium olivaceous-brown, smooth or almost
so to often indistinctly asperulate or loosely minutely verruculose,
more obvious in small terminal and intercalary conidia, under SEM
smooth or surface reticulate or with embossed stripes caused by
diminishing turgor and shriveling of tender young conidia, walls
unthickened, hila conspicuous, subdenticulate, 0.5−1.8 μm diam,
somewhat thickened and darkened-refractive; without microcyclic
conidiogenesis.
Culture characteristics: Colonies on PDA grey-olivaceous to
iron-grey, reverse iron-grey to olivaceous-black, grey-olivaceous
towards margins, velvety to loccose, margins white, glabrous to
feathery, aerial mycelium scattered, loccose, smoke-grey, growth
lat, few small to large prominent exudates, sporulation profuse.
Colonies on MEA olivaceous-grey to smoke-grey in colony centre
due to aerial mycelium and lack of sporulation, most of the colony
grey-olivaceous due to abundant sporulation, reverse olivaceousgrey to iron-grey, velvety to felty-loccose, margins white, glabrous
to feathery, growth effuse with elevated colony centre, tip of the
colony immersed, sometimes radially furrowed, without exudates.
Colonies on OA olivaceous, greenish olivaceous towards margins
and with stripes and triangles of iron-grey, reverse iron-grey to
pale greenish grey, loccose to felty-villose, margins colourless,
narrow, glabrous, aerial mycelium mainly in colony centre, loccose
to felty-villose, pale olivaceous-grey, growth lat, without exudates,
sporulation profuse.
Substrate and distribution: On wilted leaves of Ruscus; Europe
(Slovenia).
Fig. 279. Cladosporium scabrellum (CBS 126358). A. Globose conidia with nearly
smooth surface. B. Overview of conidial chains. C. Elongated conidiophores. D.
Secondary ramoconidium with large scars. E. Whorls of secondary ramoconidia
and conidia. Note the presence of four scars on the top ramoconidium and the
reticulate ornamentation of one of the conidia. F. Secondary ramoconidia of which
one elongated. G. Conidiophore with secondary ramoconidia and large scar. Scale
bars = 2 (A), 5 (D–G), 10 (B), 50(C) µm.
to conidiophores solitary and not in ropes, sometimes slightly wider
at the base of conidiophores. Conidiophores macronematous,
occasionally micronematous, solitary, arising laterally and terminally
from hyphae, erect, straight, cylindrical-oblong, neither nodulose
nor geniculate, unbranched, occasionally once branched with quite
short branches just below a septum, 40−115(−185) × 3−4 μm, at
the base up to 5 μm wide, septate, not constricted at septa, medium
olivaceous-brown, smooth or inely verruculose, walls only slightly
thickened, about 0.5 μm wide. Conidiogenous cells integrated,
mainly terminal, but also intercalary, cylindrical-oblong, neither
geniculate nor nodulose, 25−53 μm long, with up to four loci at the
apex, conspicuous, subdenticulate, 1−1.8 μm diam, thickened and
darkened-refractive. Ramoconidia occasionally formed, cylindricaloblong, up to 34 μm long, 4 μm wide, base 2−2.5 μm wide, smooth
or inely verruculose. Conidia very numerous, catenate, in densely
branched chains, branching in all directions, up to four conidia in
the unbranched terminal part, small terminal conidia subglobose to
www.studiesinmycology.org
Notes: Cladosporium scabrellum morphologically resembles C.
cladosporioides and C. tenuissimum, but in phylogenetic analyses
the three species proved to be quite distinct (see Bensch et al.
2010, ig. 1, part a vs. c), and there are some morphological
differences. Above all, C. scabrellum often forms long dense ropes,
the conidiophores are shorter and the smooth or mostly indistinctly
asperulate conidia are very numerous.
140. Cladosporium silenes Crous, IMA Fungus 2(1): 64.
2011. Fig. 280.
Holotype: UK, Pembrokeshire, Skomer Island, stems of exposed
Oberna unilora (= Silene maritima) (Caryophyllaceae) 22 Aug.
2000, A. Aptroot 49319 (CBS H-19874). Ex-type culture: CBS
109082.
Ill.: Crous et al. (2011b: 64, ig. 4).
In vivo: Ascomata occurring in exposed twigs, amphigenous,
black to dark brown, subepidermal, to 70 μm diam, visible by an
erumpent, central, periphysate ostiole, 5–10 μm; wall of 2–3 layers
of red-brown textura angularis. Asci aparaphysate, fasciculate,
bitunicate with issitunicate discharge, subsessile, obovoid to
243
BenSch et al.
Fig. 280. Cladosporium silenes (CBS H-19874). A. Ascomata on host tissue (arrows). B. Asci. C. Ascospores (arrow denotes mucoid appendage); D–F. Conidiophores with
conidial chains. A–C from Bensch et al. 2010. Scale bars = 10 µm.
broadly ellipsoid, straight to slightly curved, 8-spored, 25–35 × 10–
12 μm, with visible apical apiculus. Ascospores tri- to multiseriate,
hyaline, nonguttulate with angular inclusions, thick-walled, straight
to slightly curved, fusoid-ellipsoid with obtuse ends, medianly
1-septate, widest in middle of apical cell, not to slightly constricted
at the septum, tapering towards both ends, but slightly more to
lower end, (10–)11–13(–14) × (3–)3.5(–4) μm; turning brown once
discharged, and some containing remnants of a mucoid layer;
germinating from both ends, distorting, becoming brown and inely
verruculose.
brown, concolorous with tips of conidiophores, smooth, base not
cladosporioid, 2−2.5 μm wide, thickened, somewhat refractive.
Secondary ramoconidia aseptate, smooth, pale olivaceous-brown,
cylindrical-oblong, (8–)10–15(–20) × 3.5–4 μm. Conidia numerous,
catenate, in branched chains of up to 6 in the upper unbranched
part, branching in all directions. Intercalary conidia limoniform,
ellipsoid-ovoid, 7−8(−10) × (2−)2.5−3 μm, aseptate, with up to 3
distal hila. Small terminal conidia aseptate, subglobose, obovoid,
ovoid to limoniform, 4−5(−6) × (2.5−)3(−3.5) μm; hila darkened and
somewhat thickened, 0.5–1 μm diam.
In vitro: Mycelium consisting of branched, septate, pale to
medium brown 2(–4) μm wide hyphae, without any swellings and
constrictions, smooth to minutely verruculose, walls unthickened.
Conidiophores solitary, macronematous or micronematous, arising
terminally from ascending hyphae or laterally, straight to somewhat
lexuous, narrowly cylindrical to cylindrical-oblong, non-nodulose,
not geniculate-sinuous, 15−100(−200) × 3−4(−4.5) μm, unbranched,
pluriseptate, usually not constricted at septa, pale to medium
olivaceous-brown, smooth to minutely verruculose, especially
towards the base, walls unthickened, base sometimes swollen, up
to 8 μm wide; micronematous conidiophores shorter, unbranched,
10−30 × 3−4 μm. Conidiogenous cells integrated, usually terminal,
sometimes intercalary, cylindrical-oblong, not geniculate, nonnodulose, (10−)20−60 μm long, with up to three loci crowded at
the apex, subdenticulate to denticulate, protuberant, 2(−2.5) μm
diam, central dome mostly lat, somewhat thickened and darkenedrefractive. Ramoconidia straight to slightly curved, cylindricaloblong, 15−20(–25) × 3−4(–4.5) μm, aseptate, pale olivaceous-
Culture characteristics: Colonies after 2 wk at 24 °C spreading with
moderate aerial mycelium and smooth, lobate margins, reaching
40 mm diam after 2 wk. On MEA surface pale olivaceous-grey to
olivaceous-grey, reverse iron-grey. On OA surface grey-olivaceous
to olivaceous-grey. On PDA surface grey-olivaceous, reverse
olivaceous-grey.
244
Substrate and distribution: On Oberna; UK.
Notes: Strain CBS 109082 represents an ascospore isolate,
obtained from material of Silene maritima and initially identiied as
Mycosphaerella tassiana var. arthopyrenioides. Morphologically
the CBS strain is similar to C. cladosporioides, but is a member of
a distinct clade (C. cladosporioides s.lat. Lineage 3; see ig. 1, part
A in Bensch et al. 2010). Cladosporium silenes is the irst sexual
species known from the C. cladosporioides complex. Given that
these ascomata are rather inconspicuous (immersed, approx. 100
μm diam), it is not surprising that they have been largely overlooked
the genuS Cladosporium
in the past (Crous et al. 2011b). Cladosporium silenes differs from
C. cladosporioides in having shorter, unbranched conidiophores,
longer conidiogenous cells, shorter ramo- and intercalary conidia
(Bensch et al. 2010).
141. Cladosporium sinuosum K. Schub., C.F. Hill, Crous &
U. Braun, Stud. Mycol. 58: 141. 2007. Figs 281, 282.
Holotype: New Zealand, Te Anau, isolated from leaves of Fuchsia
excorticata (Onagraceae), 31 Jan. 2005, A. Blouin, C.F. Hill 1134A
(CBS H-19863). Ex-type culture: CBS 121629 = CPC 11839 =
ICMP 15819.
Ill.: Schubert et al. (2007b: 140–141, igs 34–35).
In vitro: Mycelium sparingly branched, 1–7 μm wide, septate,
not constricted at the septa, subhyaline to pale brown, smooth
to minutely verruculose, walls unthickened or slightly thickened,
sometimes with small swellings. Conidiophores arising laterally
from plagiotropous hyphae or terminally from ascending hyphae,
erect, more or less straight to lexuous, often once or several times
slightly to distinctly geniculate-sinuous, sometimes even zigzag-like,
nodulose with small to large lateral shoulders, shoulders somewhat
distant from each other or in close succession giving them a knotty/
gnarled appearance, unbranched or once branched, 25–260 × 5–7
μm, shoulders up to 10 μm wide, pluriseptate, septa sometimes
in short succession, not constricted at the septa, pale brown to
medium brown, smooth to minutely verruculose, walls thickened,
often distinctly two-layered, up to 1 μm thick. Conidiogenous
cells integrated, terminal or intercalary, often slightly to distinctly
geniculate-sinuous, nodulose with small to large laterally swollen
shoulders, 8–30 μm long, proliferation sympodial, with a single
or up to three conidiogenous loci, usually conined to lateral
shoulders, protuberant, often denticle-like or on the top of short
cylindrical stalk-like prolongations, 1.2–2(–2.2) μm diam, mainly
2 μm, somewhat thickened and darkened-refractive, dome often
slightly higher than the surrounding rim. Conidia solitary or in short
unbranched chains with up to three conidia, straight, obovoid, oval,
broadly ellipsoid to subcylindrical or sometimes clavate (broader at
the apex), 9–21 × (5–)6–8 μm [av. ± SD, 14.5 (± 2.5) × 6.6 (± 0.7)
μm], 0–1-septate, not constricted at the septa, septum more or less
median, pale greyish brown, densely echinulate, spines up to 1 μm
long, walls thickened, apex mostly broadly rounded or sometimes
attenuated, towards the base mostly distinctly attenuated forming a
peg-like prolongation, up to 2 μm long, hila protuberant, 1.2–2 μm
diam, mainly 2 μm, somewhat thickened and darkened-refractive;
microcyclic conidiogenesis not observed.
Culture characteristics: Colonies on PDA attaining 20 mm
diam after 14 d at 25 ºC, pale olivaceous-grey due to abundant
aerial mycelium, olivaceous-grey towards margins, iron-grey
to olivaceous-black reverse, margin regular, entire edge, aerial
mycelium abundant, cottony, dense, high, growth regular, low
convex, radially furrowed in the centre, growing deep into the agar,
with age numerous small to large prominent exudates, sporulation
sparse. Colonies on MEA attaining 16 mm diam after 14 d at 25 ºC,
white to pale smoke-grey, fawn reverse, velvety, margin undulate,
glabrous, aerial mycelium abundant, dense, high, luffy, growth
raised with elevated colony centre, laterally furrowed, without
prominent exudates. Colonies on OA attaining 18 mm diam after 14
www.studiesinmycology.org
Fig. 281. Cladosporium sinuosum (CPC 11839). Conidiophores and conidia in vitro.
Scale bar = 10 µm. K. Bensch del.
d at 25 ºC, olivaceous, white to pale olivaceous-grey in the centre
due to abundant aerial mycelium, olivaceous-grey reverse, margin
white, entire edge, glabrous, aerial mycelium loose to dense, high,
luffy to felty, growth lat to low convex, regular, without prominent
exudates, sporulating.
Substrate and distribution: Isolated from various plants; New
Zealand.
Additional specimens examined: New Zealand, Lincoln, Kimihia, Boundary Road,
isolated from Hordeum vulgare (Poaceae), 6 Dec. 2007, R. Thangavel (HAL 2237
F); isolated from Secale cereale (= ×Triticum secale) (wheat × rye hydrid), 6 Dec.
2007, R. Thangavel (HAL 2236 F).
Notes: Cladosporium sinuosum belongs to the C. herbarum
complex, but differs from the latter species (s. str.) in having slightly
to distinctly geniculate-sinuous, often zigzag-like conidiophores
and Heterosporium-like conidia, i.e. formed singly or rarely in
short unbranched chains. Morphologically comparable species
with a similar combination of such conidiophores and conidia are
unknown (Schubert et al. 2007b).
142. Cladosporium smilacicola K. Schub., Schlechtendalia
14: 77. 2006. Figs 283, 284.
Holotype: Germany, München, botanical garden, cold house,
on Smilax regelii (= S. grandifolia) (Smilacaceae), Mar. 1895,
Allescher (M-0057718), as “C. smilacis (Schwein.) Fr.”.
Lit.: Schubert (2005b: 132–134).
Ill.: Schubert (2005b: 133, ig. 64, pl. 28, igs A–G), Schubert et al.
(2006: 79, ig. 11, pl. 2, igs G–H).
In vivo: On living leaves, large areas of the leaf surface becoming
necrotic, at irst at leaf margins, later extending, on the upper
245
BenSch et al.
Fig. 282. Cladosporium sinuosum (CPC 11839). A–D. Conidiophores. E–F. Conidia. G–H. SEM photos showing details of the conidial surface ornamentation. Scale bars = 5
(H), 10 (A–G) µm.
leaf surface pale greyish brown to whitish, faded, below dingy
olivaceous-brown to pale greyish brown, partly limited by a
narrow, brown or somewhat reddish brown margin and a paler
halo. Colonies mainly hypophyllous, sometimes sparsely fruiting
on the upper leaf surface, effuse, loosely to densely caespitose,
dingy greyish brown to brown, somewhat loccose with dense
fructiication (visible with a stereomicroscope), velvety. Mycelium
internal, subcuticular to intraepidermal; hyphae branched, 2–6
μm wide, septate, with swellings and constrictions, subhyaline
to very pale olivaceous-green, smooth, thin-walled or almost so,
forming a loose network or hyphal plates. Stromata intraepidermal
and substomatal, at irst small, subglobose, later extending and
lattened, compact, 15–85 μm wide or conluent and even larger,
several layers deep, composed of swollen hyphal cells, subglobose
to somewhat angular-oblong, 5–10 μm diam, pale to medium brown,
smooth, walls somewhat thickened. Conidiophores in small to
246
somewhat larger, loose fascicles, arising from stromata, emerging
through stomata or erumpent through the cuticle, erect, straight
to slightly lexuous, cylindrical-oblong, often slightly geniculatesinuous near the apex, unbranched or usually branched, once or
several times and often near the base, 20–150 × 2.5–5 μm, septate,
pale brown to pale olivaceous-brown, somewhat darker near the
base, smooth to somewhat irregularly rough-walled, sometimes
minutely verruculose near the apex, walls somewhat thickened,
sometimes even two-layered near the base, occasionally slightly
swollen near the base, sporadically enteroblastically proliferating.
Conidiogenous cells integrated, terminal and intercalary, cylindricaloblong, slightly geniculate, 14–48 μm long, with a single or several
conidiogenous loci, often situated on small lateral shoulders,
protuberant, subdenticulate, 1–2 μm diam, dome not or only slightly
higher than the surrounding rim, thickened, darkened-refractive.
Ramoconidia sporadically occurring, broadly cylindrical-oblong, up
the genuS Cladosporium
to 26 μm long, 0(–1)-septate, with a broadly truncate, unthickened
base, 3 μm wide. Conidia in branched chains, straight, numerous
and variable in shape, subglobose, obovoid, ellipsoid, fusiform,
subcylindrical to cylindrical-oblong, 2–18(–21) × 1.5–5(–6) μm,
0–3(–4)-septate, usually not constricted at the septa, pale brown
to pale olivaceous-brown, almost smooth to often verruculose
or irregularly rough-walled, walls somewhat thickened, apex
rounded or slightly attenuated towards the apex and base, hila
protuberant, short cylindrical, truncate to slightly convex, (0.5–)1–2
μm diam, thickened, somewhat darkened-refractive; microcyclic
conidiogenesis occurring.
Substrate and distribution: On Smilax regelii; Germany.
Fig. 283. Cladosporium smilacicola (M-0057718). Symptoms, fascicle of
conidiophores and conidia in vivo. Scale bar = 10 µm. K. Bensch del.
Notes: Type material of Cladosporium smilacis, briely described
by Schweinitz (1822) as Dematium smilacis Schwein. on twigs
of Smilax sp. from North America, is in very poor condition. No
Cladosporium has been found, only few dark brown, 2–3-septate
conidia without cladosporioid hila have been observed, so that
this species has to be excluded from Cladosporium s. str., but its
taxonomic status remains unclear. Zhang et al. (2003) described
and illustrated a record on Smilax china determinated as C.
smilacis, which could not be obtained for examination.
Cladosporium smilacicola is morphologically comparable
with C. foliorum and C. oreodaphnes, but the latter species is
quite distinct in having wider, often subnodulose to nodulose
conidiophores, wider, 0–1(–3)-septate conidia with more or less
thickened, sometimes even two-layered walls and a somewhat
unusual cell structure (protoplasm aggregated so that the septa and
walls appear to be thickened, with a conspicuous, paler lumen in
the centre of the cells giving them a somewhat zonate appearance).
Cladosporium foliorum deviates in forming dense, Fusicladium-like
stromatic hyphal aggregations with sometimes irregularly lobed
cells, usually unbranched conidiophores and somewhat longer,
0–1(–2)-septate conidia (Schubert 2005b).
143. Cladosporium soldanellae Jaap, Ann. Mycol. 5: 270.
1907. Figs 285, 286.
Neotype (proposed here): Germany, Bavaria, GarmischPartenkirchen, Hammersbach, trail from Hammersbach to
“Höllentalklamm” and Höllentalanger, near Höllentalangerhütte,
wet meadow, on leaves of Soldanella alpina (Primulaceae), 23
Jun. 2006, K. Schubert, Braun, Fungi Sel. Exs. 81 (HAL 2009 F).
Isoneotypes: Braun, Fungi Sel. Exs. 81 (BPI, GZU, HMAS, IMI, KR,
KUS, LE, M, PDD, VPIR) and CBS H-19871. Ex-neotype culture:
CBS 132186 = CPC 13153.
[Type: Switzerland, Simplonhospiz, 2010 m alt., on dead leaves of
Soldanella alpina (not preserved).]
= Cladosporium stysanoides Bubák, Bot. Közlem. 15(3–4): 81. 1915.
[holotype: BPI 427476].
Fig. 284. Cladosporium smilacicola (M-0057718). A. Symptoms. B. Overview. C.
Fascicle of conidiophores. D. Tip of a conidiophore with attached conidium, with
several conidiogenous loci and hila. E. Conidiophores. F. Conidia showing surface
ornamentation. G. Conidiophores, conidia and microcyclic conidiogenesis. Scale
bars = 10 (C–G), 20 (B) µm.
www.studiesinmycology.org
Lit.: Lindau (1910: 796), Ferraris (1912: 348), Saccardo (1913a:
1368), Schubert (2005b 134–136).
Ill.: Schubert (2005b: 135, ig. 65, pl. 29, igs A–D).
Exs.: Braun, Fungi Sel. Exs. 81.
In vivo: On leaves and petioles, leaf spots formed as subcircular,
whitish or pale brown, somewhat raised discolorations. Colonies
amphigenous, scattered, punctiform, in small tufts, brown, at irst
247
BenSch et al.
truncate, hila protuberant, (1–)1.5–2(–2.5) μm diam, thickened,
somewhat darkened-refractive; microcyclic conidiogenesis not
observed.
Fig. 285. Cladosporium soldanellae (BPI 427476, holotype of C. stysanoides).
Fascicle of conidiophores and conidia in vivo. Scale bar = 10 µm. K. Bensch del.
concentrically arranged at the border of the raised discolorations,
later extending. Mycelium internal, subcuticular to intraepidermal;
hyphae branched, 1.5–8 μm wide, septate, without swellings and
constrictions, subhyaline or even hyaline to very pale olivaceousgreen, smooth, walls not or slightly thickened, radiating, forming
hyphal plates. Stromata intraepidermal, sometimes substomatal,
subglobose to somewhat oblong-angular, usually effuse, 40–150
μm wide, sometimes conluent and larger, several layers deep,
compact, composed of subglobose to angular-oblong, polygonal
cells, 4–9 μm wide, medium to dark brown, smooth, thick-walled.
Conidiophores mostly in large, dense fascicles, numerous, arising
from stromata, emerging through stomata or erumpent through the
cuticle, erect, straight, especially at the base of the conidiomata
more or less parallel, synnema-like, somewhat lexuous towards
the apex, short cylindrical to cylindrical-oblong, non-geniculate or
rarely slightly geniculate near the apex, non-nodulose or sometimes
subnodulose, unbranched, rarely branched, 12–180 × 3.5–7 μm,
0–5-septate, pale to medium olivaceous-brown, paler towards
the apex, smooth or almost so to minutely verruculose, walls
slightly thickened, one-layered, rarely two-layered near the base,
protoplasm of the cells somewhat aggregated at the septa which
appear to be thickened, sometimes somewhat swollen at the base,
slightly attenuated towards the apex, sporadically enteroblastically
proliferating. Conidiogenous cells integrated, usually terminal,
occasionally intercalary, or short conidiophores reduced to
conidiogenous cells, cylindrical-oblong, sometimes slightly
geniculate, 12–40 μm long, mostly with a single conidiogenous
locus, sometimes with few loci, protuberant, 1.5–2(–2.5) μm diam,
thickened, somewhat darkened-refractive. Conidia solitary or
in unbranched chains, obovoid, ellipsoid to cylindrical, straight,
6.5–26 × 4–6(–7) μm, 0(–1)-septate, sometimes slightly constricted
at the septa, pale to medium olivaceous-brown, concolorous with
conidiophores, minutely verruculose, walls slightly thickened, apex
rounded or attenuated towards apex and base, base often broadly
248
In vitro: Mycelium immersed and supericial; hyphae branched,
1–4 μm wide, single cells distinctly swollen, up to 7 μm wide,
pluriseptate, sometimes constricted at septa, subhyaline to pale
or medium brown or olivaceous-brown, smooth, walls unthickened
or slightly thickened, about 0.5 μm wide. Conidiophores solitary
or in loose groups of 2–3, arising terminally and laterally from
hyphae, erect, straight to more or less lexuous, narrowly
cylindrical-oblong but due to swellings and constrictions sometimes
irregular in outline, sometimes slightly geniculate towards the
apex or subnodulose, unbranched or once branched, 25–200
× 3–4.5(–5) μm, pluriseptate, pale to medium olivaceous-brown,
smooth or partly minutely verruculose, walls unthickened or
somewhat thickened, about 0.5 μm wide. Conidiogenous cells
integrated, mainly terminally but also intercalary, cylindrical-oblong,
sometimes geniculate or subnodulose, 10–55 μm long, smooth
or often somewhat verruculose, with up to four loci per cell, loci
conspicuous, protuberant, 1–1.5(–2) μm diam, thickened and
darkened-refractive. Ramoconidia occasionally formed, up to 32
μm long, often minutely verruculose, base 3–3.5 μm wide. Conidia
catenate, numerous, in branched chains, up to four conidia in the
terminal unbranched part of the chain, straight, small terminal
conidia obovoid to limoniform or subglobose, 3–8 × 2–3 μm [av.
(± SD) 4.7 (± 1.7) × 2.6 (± 0.4) μm], aseptate, intercalary conidia
limoniform, ellipsoid to subcylindrical, 5.5–17 × (2.5–)3–4(–4.5) μm
[av. (± SD) 9.9 (± 3.3) × 3.4 (± 0.4) μm], 0–1(–2)-septate, with
1–2 distal scars, secondary ramoconidia ellipsoid to subcylindrical
or cylindrical, (7.5–)11–27(–31) × 3–4(–4.5) μm [av. (± SD) 19.1
(± 6.5) × 3.9 (± 0.7) μm], 0–3-septate, septa often not very
conspicuous, with 2–3(–4) distal scars, almost smooth to minutely
verruculose or loosely verruculose, walls unthickened or almost
so, hila protuberant, (0.5–)1–1.5(–2) μm diam, thickened and
darkened-refractive; microcyclic conidiogenesis occurring; conidia
sometimes germinating.
Culture characteristics: Colonies on PDA attaining 8–20 μm after
14 days, olivaceous-grey to iron-grey, olivaceous-black at margins,
smoke-grey due to aerial mycelium, reverse olivaceous-black,
velvety to luffy-wooly, margins white, very narrow, somewhat
feathery, regular to somewhat irregular, aerial mycelium loose to
dense, high, luffy, growth low convex to convex, without prominent
exudates, sporulation profuse. Colonies on MEA reaching 12–21
mm, smoke-grey to grey-olivaceous due to a profuse sporulation,
iron-grey at margins, reverse olivaceous-grey to iron-grey, luffy,
margins colourless or white, narrow, glabrous to somewhat feathery,
aerial mycelium abundant, high, luffy, dense, growth convex with
elevated and furrowed parts, without prominent exudates. Colonies
on OA attaining 8–18 mm, olivaceous-grey, iron-grey at margins,
smoke-grey or whitish due to aerial mycelium, reverse olivaceousblack, velvety to powdery, margins iron-grey, somewhat feathery,
irregular, aerial mycelium diffuse to dense forming few large spots
of luffy dense hyphae, whitish, high, growth lat, without prominent
exudates, sporulation profuse.
Substrate and distribution: On Soldanella spp.; Europe – Soldanella
alpina (Germany, Italy, Montenegro, Switzerland), S. hungarica
subsp. major (Romania), S. montana (Romania), S. pusilla
(Romania).
the genuS Cladosporium
Fig. 286. Cladosporium soldanellae in vivo (BPI 427476, holotype of C. stysanoides) and in vitro (CPC 13153 = CBS 132186, ex-neotype culture of C. soldanellae). A. Overview,
fascicle of conidiophores B. Stromata and mycelium. C. Conidiophores. D. Conidium. E–K. Conidiophores and conidial chains in vitro. Scale bars = 10 (B–K), 50 (A) µm.
www.studiesinmycology.org
249
BenSch et al.
Additional specimen examined: Montenegro, Durmitor, Lokvice, ca. 2200 m, on
Soldanella alpina, 15 Aug. 1904, F. Bubák (BPI 427476, holotype of C. stysanoides).
Notes: Type material of Cladosporium stysanoides was re-examined
and turned out to be a true, well-characterised Cladosporium
species. The original diagnosis of C. soldanellae (Jaap 1907),
recorded on leaves of Soldanella alpina from Switzerland, has
been compared with this species and proved to be very close to
C. stysanoides. Jaap (1907) described the colonies as punctiform,
at irst concentrically arranged at pale subcircular leaf spots, later
extended, with unbranched conidiophores in dense fascicles, 100–
175 × 4–5 μm, and 0–1-septate, cylindrical-oblong conidia, 13–15
× 4–5 μm. Although type material of C. soldanellae could not be
traced, neither at herb. B nor HBG, both species are considered
to be conspeciic. Cladosporium soldanellae, which antedates
C. stysanoides, has priority according to the Code of Botanical
Nomenclature, and therefore, C. stysanoides is reduced to
synonymy with the latter species. Type material of C. soldanellae is
probably not preserved, so a neotype is proposed. Ferraris (1912)
mentioned a record from Tyrol, Italy. Soldanella hungarica ssp.
major, S. montana and S. pusilla have been reported as additional
hosts from Romania (Bontea 1985).
Cladosporium soldanellae supericially resembles the saprobic
C. herbarum but deviates in having densely fasciculate, synnemalike conidiophores arranged in punctiform, non-caespitose tufts,
which usually do not have terminal and intercalar swellings with
conidiogenous loci conined to them, and 0(–1)-septate conidia.
Phylogenetically it is closely related to C. ossifragi but the
latter species differs in having closely verruculose to echinulate,
(0–)1–5(–7)-septate, wider conidia [(5–)7–10(–12) μm]. Its
conidiophores in vivo are arranged in fascicles but never synnemalike as in C. soldanellae.
144. Cladosporium sphaerospermum Penz., Michelia
2(8): 473. 1882. Figs 287–289.
Neotype (designated by Zalar et al. 2007): Isolated from nail of
man, 1949, R.W. Zappey (CBS H-19738). Ex-neotype culture: CBS
193.54 = ATCC 11289 = IMI 049637.
[Type: Italy, Padova, on faded leaves and stems of Citrus sp.
(Rutaceae), Feb. 1882, O. Penzig (not preserved)].
= Cladosporium papyricola Berk. & Broome, Trans. Linn. Soc., Ser. 2, Bot., 2:
68. 1883, as "papyricolor" [holotype: K 121565], syn. nov.
= Torula lichenopsis Höhn., Denkschr. Kaiserl. Akad. Wiss., Math.-Naturwiss.
Kl. 83: 36. 1927. [holotype: FH-Höhnel 1275].
= Cladosporium hibisci Reichert, Bot. Jahrb. Syst. 56: 721, 1921 [holotype: B
700006553], syn. nov.
Lit.: Saccardo (1886: 355), Lindau (1907: 826), Ferraris (1912:
345), De Vries (1952: 81), Yamamoto (1959: 3), Ellis (1971: 315),
Hawksworth (1979: 287), Ellis & Ellis (1985: 290, 1988), Wang &
Zabel (1990: 200), Domsch et al. (1980: 209), Ho et al. (1999: 133,
as C. lignicola, 139), de Hoog et al. (2000: 591), Samson et al. (2000:
114, 2001: 340), Zhang et al. (2003: 156–158), Heuchert et al. (2005:
50), Zalar et al. (2007: 177–179). Dugan et al. (2008: 9–16).
Ill.: De Vries (1952: 82, ig. 18), Yamamoto (1959: 2, igs 5–8),
Minoura (1966: 141, ig. 5C), Ellis (1971: 316, ig. 218 A), Domsch et
al. (1980: 209, ig. 85), Ho et al. (1999: 133, ig. 27; 141, igs 42–43),
de Hoog et al. (2000: 591–592, igs), Samson et al. (2000: 114, ig.
51; 115, pl. 49), Zhang et al. (2003: 157, ig. 107), Zalar et al. (2007:
166, ig. 5 g, 178, ig. 12). Dugan et al. (2008: 13–14, igs 2–3).
250
In vivo: Colonies punctiform to effuse, sometimes larger patches,
loose to dense, mostly rather thin, medium to dark olivaceous to
greenish black. Mycelium immersed, sometimes also external.
Hyphae branched, septate, 1.5–4 μm wide, subhyaline, olivaceous
to brown, smooth or almost so to minutely verruculose, wall thin to
somewhat thickened. Conidiophores solitary or loosely aggregated,
arising from immersed or superical hyphae, lateral or terminal,
erect to decumbent, usually macronematous, but occasionaly also
micronematous, subcylindrical-iliform, not or barely geniculatesinuous, non-nodulose, micronematous conidiophores simple,
macronematous ones unbranched or frequently branched, once
branched to variously branched, 10–300(–500) × 2–5 μm, short
micronematous conidiophores aseptate or sparingly septate,
macronematous ones pluriseptate throughout, pale olivaceous to
medium brown or olivaceous-brown, wall ≤ 1 μm wide, smooth to
minutely verruculose. Conidiogenous cells integrated, terminal,
occasionally intercalary or pleurogenous, 10–30 μm long, little
differentiated, without swellings, non-geniculate, occasionally
subdenticulate caused by the protuberant conidiogenous loci,
which are somewhat thickened and darkened, 1–1.5(–2) μm diam.
Ramoconidia abundant, cylindrical-subcylindrical, 12–35 × 3–5
μm, (0–)1–3(–4)-septate, pale to medium olivaceous or brown, wall
≤ 1 μm wide, smooth or almost so. Conidia in branched chains,
small terminal conidia globose or subglobose, 2–5 × 2–4 μm, apex
rounded, base rounded to slightly attenuated, intercalary conidia
subglobose to broadly ellipsoid-ovoid, 4–10 × 3–5 μm, 0–1-septate,
subhyaline, pale olivaceous to olivaceous-brown, thin-walled (ca.
0.5 μm), almost smooth to verruculose (light microscopy), warts
easily visible in air bubbles, secondary ramoconidia subglobose to
subcylindrical, (4–)6–20 × (2.5–)3–4.5(–5) μm, 0–2(–3)-septate,
pale to medium olivaceous to olivaceous-brown, wall ≤ 1 μm wide,
smooth or almost so, hila often somewhat protuberant, coronate,
0.5–1.5 diam.
In vitro: Mycelium partly submerged, partly supericial; hyphae
sparingly branched, 1–3 μm wide, septate, pale to pale medium
olivaceous-brown, smooth to sometimes minutely verruculose,
walls slightly thickened, not enveloped in polysaccharide-like
material. Conidiophores micronematous and macronematous,
arising terminally and laterally from hyphae, erect or ascending,
straight to slightly lexuous. Macronematous conidiophores
cylindrical-oblong, neither geniculate nor nodulose, unbranched
or branched, (10–)45–130(–300) × 2.5–4.5(–6) μm, pluriseptate,
with relatively dense septation (cells mostly 4.5–23 μm long),
septa darkened and somewhat thickened, pale medium to
medium olivaceous-brown, smooth to minutely verruculose, walls
thickened. Conidiogenous cells integrated, terminal, sometimes
intercalary, cylindrical, usually short, 6–18 μm long, proliferation
sympodial, with a single or few apical scars, loci protuberant,
denticulate, 0.8–1.5 μm diam, thickened and darkened-refractive.
Micronematous conidiophores iliform to narrowly cylindricaloblong, up to 80 μm long or even longer, 1–2 μm wide, pluriseptate,
not that densely septate as macronematous ones, septa also
somewhat darkened and thickened, pale to medium olivaceousbrown, walls almost unthickened. Conidiogenous cells integrated,
terminal and intercalary, short cylindrical, 9–27 μm long, with a few
subdenticulate loci, 0.5–0.8 μm diam, thickened and darkenedrefractive. Ramoconidia often formed, cylindrical, (11.5–)20.5–40(–
48) × (2.5–)3(–3.5) μm, with up to ive septa, base broadly truncate,
2 μm wide, slightly thickened and somewhat darkened-refractive,
but not coronate. Conidia catenate, in branched chains, branching
in all directions, with up to six conidia in the unbranched parts,
the genuS Cladosporium
Fig. 287. Cladosporium sphaerospermum (CBS 19454) (from Zalar et al. 2007). A–D. Colony surface grown on PDA (A), OA (B), MEA (C) and MEA plus 5 % NaCl (D) of strains
incubated for 14 d at 25 °C in darkness. E–F. Habit of conidiophores. G–I. Ramoconidia and conidia. E–I. All from 7-d-old SNA slide cultures. A, C–D, F–H, from CBS 193.54
(ex-neotype strain); B, from EXF-738; E, EXF-455; I, EXF-458. Scale bars = 10 (G–I), 50 (F), 100 (E) µm.
straight, small terminal conidia globose to subglobose, sometimes
ovoid, (2–)3–5(–7) × (2–)3–3.5 μm [av. (± SD) 4.1 (± 0.7) × 3.2 (±
0.3) μm], aseptate, minutely verruculose to verrucose, narrower at
both ends, intercalary conidia with 1–2 apical hila subglobose, ovoid
to ellipsoid, 4.5–8.5 × 3–4(–4.5) μm [av. (± SD) 6.5 (± 1.6) × 3.6 (±
0.3) μm], aseptate, attenuated towards apex and base, secondary
ramoconidia ellipsoid to cylindrical, 8–22(–37.5) × (2–)3–4(–5) μm
[av. (± SD) 15.4 (± 5.1) × 3.6 (± 0.5) μm], 0–3(–4)-septate, not
constricted at septa, but septa somewhat darkened and thickened,
www.studiesinmycology.org
pale to usually medium olivaceous-brown, sometimes dark brown,
smooth to minutely verruculose, walls thickened, with up to six
pronounced, denticulate distal hila, 0.8–1.5 μm diam, sometimes
loci situated at the end of protuberant, short, terminal projections,
1–2 μm long or even longer in secondary ramoconidia with beaklike ends, sometimes alternarioid, obclavate, subrostrate (not
observed when cultivated on SNA after 7 d, but on PDA and MEA),
thickened and darkened-refractive; microcyclic conidiogenesis not
observed.
251
BenSch et al.
Fig. 288. Cladosporium sphaerospermum (NRRL 8131) (from Dugan et al. 2008). A–H. Conidiophores at various stages of development, showing their characteristic branching
patterns, ramoconidia, secondary ramoconidia, intercalary conidia, and small, terminal conidia (all on SNA). I. Conidiophore with alternarioid secondary ramoconium (arrow),
formed on MEA. J, K. Secondary ramoconidia and intercalary conidia (note older intercalary conidia, which become dark brown and globose). Scale bars = 10 µm.
Culture characteristics: Colonies on PDA reaching 21–50 mm
diam in 14 d at 25 °C, grey-olivaceous or greenish olivaceous,
reverse dark grey-olivaceous, iron-grey or greyish-blue, velvety,
margin white, regular, narrow, somewhat feathery, aerial
mycelium absent or sparse, growth flat with an elevated colony
centre, numerous prominent exudates formed, sporulating,
some strains release green soluble pigment into the agar.
Colonies on OA reaching 21–38 mm diam, dark grey-olivaceous,
olivaceous or olivaceous-grey due to profuse sporulation,
reverse greenish grey, velvety, aerial mycelium absent,
growth flat with papillate surface, without prominent exudates.
252
Colonies on MEA attaining 15–45 mm diam, grey-olivaceous to
olivaceous-grey, reverse olivaceous-grey to iron-grey, powdery,
velvety, margin colourless or white, feathery, regular, radially
furrowed, aerial mycelium almost absent, growth low convex
with elevated colony centre, centre often wrinkled forming a
crater-like structure, without prominent exudates, sporulation
profuse. Colonies on MEA with 5 % NaCl growing faster than
on other media, reaching 31–60 mm diam, mainly olive, either
being almost flat or radially furrowed, with margin of superficial
mycelium, sporulation dense, reverse ochraceous or dark
green. Maximum tolerated salt concentration: On MEA + 20 %
the genuS Cladosporium
Fig. 289. Cladosporium sphaerospermum (NRRL 8131) (from Dugan et al. 2008).
Scanning electron micrographs of Cladosporium sphaerospermum NRRL 8131.
A, B. Branching chains of conidia, showing conidiogenous loci with disjunctors
(arrows). C. Apex of conidiophore with conidiogenous scar in proile (arrow). D.
Two conidiogenous loci at apex of a secondary ramoconidium, the upper (arrow)
clearly coronate. E. Two conidiogenous loci at apex of a conidiophore, the one
facing the viewer is clearly coronate (arrow). F. Two conidiogenous loci (arrows) at
apex of a secondary ramoconidium are coronate. Scale bars = 1 (D), 1.25 (F), 2.5
(A–C), 5 (E) µm.
NaCl 89 % of all strains tested develop colonies after 7 d, 96 %
after 14 d. Cardinal temperatures: No growth at 4 °C, optimum
at 25 °C, maximum at 30 °C, no growth at 37 °C.
Substrates and distribution: Occurring as secondary invader on
numerous plants, saprobic on dead leaves, stems, wood and other
plant organs, isolated from outdoor and indoor air, soil, hypersaline
water, indoor wet cells, foodstuffs and other organic matter, paint,
silicone, textiles and occasionally isolated from man and animals
(nails, nasal mucus, etc.); cosmopolitan.
Additional specimens examined: Antarctica, Edmonson Point (North Valley),
near the lake, isolated from soil (ornithogenic), 24 Jan. 1995, S. Onofri 8 (IMI
377828). Dominican Republic, salterns, isol. from hypersaline water (EXF715). Egypt, near Siut, on dry stems of Abelmoschus esculentus (Malvaceae),
Oct. 1822/25, Ehrenberg (B 700006553, holotype of C. hibisci). Germany,
Hessen, Frankfurt/Main, on Euonymus sp. cult. (Celastraceae), Jan. 1997, ex
“Planzenschutzamt Frankfurt” (HAL), together with Erysiphe euonymicola. Israel,
Dead Sea, isol. from hypersaline water (EXF-1061, EXF-1726); Eilat salterns,
isol. from hypersaline water (EXF-1069, EXF-1732). New Zealand, Auckland,
isolated from Onychiurus pseudoimetarius (Collembola), 29 Jan. 1969, J. Young
10 (IMI 137456), as C. cladosporioides; Henderson, on dead leaves of Kalanchoë
fedtschenkoi (Crassulaceae), 17 Jun. 1980, P.J. Brook (PDD 41029); Campbell
Island, on ground litter (Dracophyllum sp.), 6 Mar. 2000, S.L. Stephenson (PDD
74350). Slovenia, isol. from a bathroom (EXF-738, EXF-739, EXF-962, EXF965); Sečovlje salterns, isol. from hypersaline water (EXF-131 = MZKI B-1005,
EXF-328, EXF-385, EXF-446, EXF-455, EXF-458, EXF-461, EXF-464, EXF-465,
EXF-598, EXF-644, EXF-645, EXF-649, EXF-781 = MZKI B-899, EXF-788).
Spain, Barcelona, Salines de la Trinitat, isol. from hypersaline water (CBS 102045
= EXF-2524 = MZKI B-1066). Russia, Kaliningrad, isol. from gardening peat
substrate (CPC 10944). UK, Surrey, Kew, isolated from Arundo sp. (Poaceae),
Sep. 1943, G.R. Bisby (IMI 25324). USA, isol. from Carya illinoinensis leaf scale
(Juglandaceae) (CBS 109.14 = ATCC 36950); isolated from wood (CBS H-20086,
HAL 1846 F, dried culture ex ATCC 38493; cultures ATCC 38493 = CBS 117728
= CPC 12098 = NRRL 8131); Philadelphia, QM Depot, on cotton duck, 21 Nov.
1951, E.T. Reese (PH 01020458, as C. herbarum).
Notes: Cladosporium sphaerospermum was described by Penzig
(1882) from decaying Citrus leaves and branches in Italy. He
www.studiesinmycology.org
described C. sphaerospermum as a species with (i) branched,
septate and dark conidiophores having a length of 150–300 μm and
a width of the main conidiophore stipe of 3.5–4 μm, (ii) spherical
to ellipsoid, acrogenously formed conidia of 3.4–4 μm diam, and
(iii) ramoconidia of 6–14 × 3.5–4 μm. Penzig’s original material
is not known to be preserved. Later, a culture derived from CBS
193.54, originating from a human nail, was accepted as typical of
C. sphaerospermum. However, de Vries (1952), incorrectly cited
it as “lectotype”, and thus the same specimen was designated
as neotype in Zalar et al. (2007), with the derived culture (CBS
193.54) used as ex-neotype strain. Zalar et al. (2007) consider
C. sphaerospermum as halo- or osmotolerant. Although C.
sphaerospermum has commonly been isolated from osmotically
stressed environments, it is also known from non-stressed niches.
Cladosporium sphaerospermum is a cosmopolitan species that has
been studied from the perspectives of phylogeny, halo-tolerance and
general ecology (summarised in Zalar et al. 2007), biodegradative
capacities (e.g., Weber et al. 1995, Prenafeta-Boldú et al. 2001,
Potin et al. 2004, Nieves-Rivera et al. 2006, Kim et al. 2007), and
clinical aspects (summarized in de Hoog et al. 2000 and Zalar et al.
2007). Hardly any reports are available unambiguously proving that
C. sphaerospermum is a human pathogen. It is therefore possible
that CBS 193.54 was not involved in any disease process but rather
occurred as a contaminant on dry nail material. Cladosporium
sphaerospermum is a phylogenetically well-delineated species
(see Fig. 5 and Zalar et al. 2007).
The only record of this species in connection with a fungal
substrate was published by Hawksworth (1979) who reduced
Torula lichenopsis, described from crustose lichen, to synonymy
with C. sphaerospermum.
Type material of C. hibisci has been examined and proved to be
identical with C. sphaerospermum (conidia verruculose, globose or
subglobose, brown; secondary ramoconidia 0–3-septate). Zhang
et al. (2003) cited collections of “C. hibisci” on Hibiscus syriacus
(China, Shaanxi) and H. tiliaceus (China, Heilongjiang). The identity
of these samples is, however, quite unclear. Klotzsch, Herb. Viv.
Mycol. 187 (HBG) is an unusual C. sphaerospermum-like collection
with long, occasionally branched conidiophores and globose,
verruculose, terminal conidia, 3–5 μm diam, but the conidiophores
are up to 10 μm wide.
The North American strain (NRRL 8131) is C. sphaerospermum,
but differs in morphology from previously known isolates of that
species (Dugan et al. 2008). It is easily distinguishable from C.
herbarum (including C. lignicola) and all other known species of
Cladosporium s. str., by having obclavate, short rostrate, sometimes
“alternarioid” conidia. Individual conidia often conformed to the
spherical shape generally typical of isolates of C. sphaerospermum,
but such conidia of NRRL 8131 could be somewhat larger than the
upper limits of 4(–7) × 3.5(–4.5) μm given for C. sphaerospermum
in Zalar et al. (2007). Furthermore, the conidiophores are at irst
consistently micronematous, much later they may become more
macronematous, and they are usually unbranched. The conidiophores
in other isolates of C. sphaerospermum are often branched in vivo
as well as in vitro. However, not only did NRRL 8131 cluster with
strains of C. sphaerospermum (see Dugan et al. 2008, ig. 1), but
the neotype of C. sphaerospermum (CBS 193.54) occasionally
displayed subrostrate “beaks” on ramoconidia (e.g., ig. 5G in Zalar
et al. 2007). Because sequence data conclusively place NRRL 8131
into C. sphaerospermum (see Dugan et al. 2008, ig. 1) and because
the subrostrate “beaks” could also be located in the neotype, the
authors refrained from designating the morphologically distinct NRRL
8131 as a new variety. In their treatment of C. sphaerospermum-like
253
BenSch et al.
Fig. 290. Cladosporium spinulosum (CPC 12040) (from Zalar et al. 2007). A–D. Colony surface grown on PDA (A), OA (B), MEA (C) and MEA plus 5 % NaCl (D) of strains
incubated for 14 d at 25 ºC in darkness. E. Habit of conidiophores. F–J. Conidiophores. K–L. Conidia (also visible in I–J). E–L. All from 7-d-old SNA slide cultures. A–L, from
EXF-334 (ex-type strain). Scale bars = 10 (G–L), 30 (F), 100 (E) µm.
species (Zalar et al. 2007) some variation was observed in the ITS
sequence data of all members studied, suggesting that they may not
present a single monophyletic group, but could belong to a species
complex within Cladosporium.
145. Cladosporium spinulosum Zalar, de Hoog & GundeCimerman, Stud. Mycol. 58: 180. 2007. Figs 290, 291.
Holotype: Slovenia, Sečovlje salterns, isolated from hypersaline
water, Feb. 1999, S. Sonjak (CBS H-19796). Ex-type culture: EXF334 = CBS 119907.
Ill.: Zalar et al. (2007: 166, Fig. 5 h, 179, Fig. 13).
254
In vitro: Mycelium immersed and supericial; hyphae unbranched
or sparingly branched, 1–3.5 μm wide, septate, usually without
swellings and constrictions, subhyaline, smooth or almost so to
somewhat irregularly rough-walled, walls unthickened, sometimes
enveloped in polysaccharide-like material. Conidiophores
solitary, arising laterally from plagiotropous hyphae or terminally
from ascending hyphae, erect or ascending, straight to more or
less lexuous, cylindrical, sometimes slightly geniculate, nonnodulose but loci sometimes situated on small lateral shoulders,
unbranched or branched, (10–)25–110(–155) × (2–)3–4(–5) μm,
0–6(–9)-septate (cells mostly 6–20 μm long), not constricted
at septa, pale to medium olivaceous-brown or brown, smooth,
sometimes irregularly rough-walled to verrucose near the base,
walls somewhat thickened, about 0.5 μm wide, somewhat
tapering towards the apex. Conidiogenous cells integrated,
the genuS Cladosporium
Fig. 291. Cladosporium spinulosum (CPC 12040). A. Overview on agar surface with conidiophores arising from the surface. The spore clusters on the conidiophore are very
compact. Note several simple, tubular conidiophore ends. The inset shows details of a conidium showing two pronounced hila and a unique, very distinct ornamentation on the
cell wall. B. Conidiophore with globose or subsphaerical secondary ramoconidia and conidia. Note the newly forming cells and hila. C. Two conidiophores. D. Details of spores
and spore formation. E. The end of a conidiophore and two scars. Scale bars = 1 (A inset), 5 (B, D–E), 10 (C), 20 (A) µm.
terminal or intercalary, cylindrical, sometimes slightly geniculate,
10–30 μm long, producing sympodial clusters of pronounced
denticles at their distal ends, sometimes situated on small lateral
shoulders or short lateral prolongations, loci protuberant, 0.8–1.2
μm diam, thickened and darkened-refractive. Ramoconidia rarely
formed. Conidia catenate, in branched chains, branching in all
directions, up to four conidia in the unbranched parts, straight,
small terminal and intercalary conidia globose to subsphaerical,
4–7(–8) × 3–4.5(–5) μm [av. (± SD) 5.3 (± 1.0) × 3.6 (± 0.4)],
aseptate, secondary ramoconidia with up to four distal scars
subsphaerical, ovoid, ellipsoid to subcylindrical, occasionally
obclavate, (6–)7–15(–18) × 4–5(–6) μm [av. (± SD)11.1 (± 3.3)
× 4.3 (± 0.6)], 0–1-septate, usually aseptate, not constricted at
the median septum, pale olivaceous to pale olivaceous-brown,
conspicuously digitate, with up to 1.3 μm long projections having
parallel sides and blunt ends, loose to moderately dense, hila
sometimes situated on small stalk-like elongations, protuberant,
0.8–1.2 μm diam, thickened and darkened-refractive; microcyclic
conidiogenesis occurring.
www.studiesinmycology.org
Culture characteristics: Colonies on PDA reaching 20–30 mm
diam, dull green to olivaceous-grey due to profuse sporulation,
reverse olivaceous-black, velvety, either with a white and regular
margin or margin narrow, undulate and feathery, colony arachnoid
towards margins, aerial mycelium sparse, growth lat with elevated
and distinctly wrinkled and folded colony centre, without prominent
exudates, growth deep into the agar. Colonies on OA reaching 20–
25 mm diam, olivaceous-grey, iron-grey reverse, margin narrow,
white, glabrous, entire edge, aerial mycelium sparse, growth lat,
radially furrowed or wrinkled in colony centre, without prominent
exudates, sporulation profuse. Colonies on MEA reaching 17–28
mm diam, olivaceous-grey to grey-olivaceous due to profuse
sporulation, iron-grey towards margins, reverse iron-grey, velvety,
margin white, narrow, crenate, radially furrowed forming a craterlike structure, aerial mycelium sparse, diffuse, growth raised to
convex with distinctly wrinkled and folded colony centre, without
prominent exudates, sporulation profuse. Colonies on MEA with 5
% NaCl reaching 12–18 mm diam, of different colours, greenish
grey (29D2), greyish green (29D5) to dark green (29F6); colony
appearance variable, mostly either being almost lat with immersed
255
BenSch et al.
146. Cladosporium spongiosum Berk. & M.A. Curtis, J.
Linn. Soc., Bot. 10(46): 362. 1869. Figs 292, 293.
≡ Helminthosporium spongiosum (Berk. & M.A. Curtis) Cif., Atti Ist. Bot.
Lab. Crittog. Univ. Pavia, Ser. 5, 19: 109. 1962.
Holotype: Cuba, on inlorescences of Cenchrus sp. (Poaceae), C.
Wright, No. 287 (K 121570).
Lit.: Saccardo (1886: 365), Ellis (1971: 317), Schubert (2005b:
136–138), Heuchert et al. (2005: 57).
Ill.: Ellis (1971: 316, ig. 218 B), Schubert (2005b: 138, ig. 66, Pl.
27, igs G–H).
Fig. 292. Cladosporium spongiosum (K 121570). Conidiophores and conidia in vivo.
Scale bar = 10 µm. K. Bensch del.
colony centre or radially furrowed, with white to dark green margin
consisting of supericial mycelium; sporulation dense. Reverse pale
to dark green. Maximum tolerated salt concentration: On MEA + 17
% NaCl, two of three strains tested developed colonies after 14 d.
Cardinal temperatures: Growth at 4 °C, optimum and maximum at
25 °C (17–28 mm). No growth at 30 °C.
Substrates and distribution: Hypersaline water; Europe (Slovenia).
Additional specimen examined: Slovenia, Sečovlje salterns, isolated from
hypersaline water (EXF-382).
Notes: Cladosporium spinulosum is a member of the C. herbarum
species complex although its globoid conidia are reminiscent of C.
sphaerospermum. Within Cladosporium, the species is unique in
having conspicuously digitate conidia and ramoconidia. The two
strains are differing in the size of conidia. The average size of
conidia in EXF-334 is 6.2 (± 0.9) × 4.2 (± 0.5) μm, and in EXF-382
it is 3.9 (± 0.6) × 3.3 (± 0.4) μm (Schubert et al. 2007b).
In vivo: Colonies on still closed, dried inlorescences, effuse,
dense, dark olivaceous-brown to almost blackish, sometimes
crustaceous, densely felt-like. Mycelium internal and external,
supericial; hyphae branched, 2–7(–9) μm wide, septate, often with
swellings and constrictions, pale to medium brown or olivaceousbrown, smooth, walls only slightly thickened, somewhat swollen at
the base of the conidiophores. Stromata absent. Conidiophores
solitary, arising from internal and external plagiotropous hyphae,
terminal and lateral, erect, straight to somewhat lexuous, slightly
geniculate-sinuous towards the apex, unbranched or sometimes
branched, 5–90 × 3–5(–6) μm, septate, often slightly constricted
at the septa, pale to medium yellowish brown or olivaceous-brown,
somewhat paler towards the apex, smooth, walls slightly thickened,
one-layered, sometimes growing like and confusable with hyphae.
Conidiogenous cells integrated, terminal and intercalary, cylindrical
to somewhat geniculate, 4–35 μm long, proliferation sympodial,
with several, subdenticulate conidiogenous loci, protuberant, short
cylindrical, truncate to obconically truncate, 0.5–1.5(–2.5) μm diam,
thickened, refractive to somewhat darkened. Conidia catenate, in
unbranched or branched chains, straight to slightly curved, very
variable in shape and size, small conidia subglobose, ovoid,
ellipsoid, 3–13 × 2–5 μm, 0–1-septate, larger conidia ellipsoid,
fusiform, subcylindrical or cylindrical, 6–41(–60) × 3.5–5.5(–7)
μm, 0–4(–7)-septate, commonly 3-septate, sometimes slightly
constricted at the septa, septa often thickened and distinctly
darkened, pale brown to medium yellowish brown, smooth, walls
somewhat thickened, apex rounded or mostly attenuated towards
the apex and base, hila protuberant, short cylindrical, obconically
truncate to slightly convex, 0.5–1.5(–2.5) μm diam, conspicuously
differentiated in central dome and periclinal rim, thickened,
refractive to darkened; microcyclic conidiogenesis occasionally
occurring.
Fig. 293. Cladosporium spongiosum (K 121570). A. Conidia. B. Conidia with distinctly thickened and darkened septa. Scale bars = 10 (B), 20 (A) µm.
256
the genuS Cladosporium
Substrates and distribution: On Cenchrus and Chaetochloa spp.
(Poaceae); Caribbean – Cenchrus sp. (Cuba), Chaetochloa setosa
(Haiti).
Additional specimen examined: Haiti, near St. Michel Caverus, Dept. du Nord, on
Chaetochloa setosa, 5 Dec. 1925, E.C. Leonard 7759 (F).
Notes: Berkeley (1869) mentioned Setaria as a second host
genus and compared the habit of his newly described species
with Helminthosporium ravenelii M.A. Curtis. Ciferri (1962) did not
examine type material of Cladosporium spongiosum, but rather
based his new combination on material collected by himself on
Cenchrus echinatus in the Dominican Republic (in comparing it
with the very brief description given by Berkeley and Curtis). He
described wider conidiophores, 6–8.5 μm, and solitary, elliptic or
subfusate, wider conidia with acuminate ends, with the free end as
a rule more acutate than the basal cell, 20–40.5 × 6–10 μm, which
do not agree with the description given above. A re-examination of
the type material showed C. spongiosum to be a genuine member
of Cladosporium s. str. The conidia with its distinctly darkened
and thickened septa remind one of C. episclerotiale, but the latter,
fungicolous species is quite distinct in having much longer and,
above all, wider conidiophores, (22–)97–295(–322) × 5–8(–10)
μm, shorter and wider conidia, with thickened, mostly distinctly
two-layered walls and somewhat wider conidiogenous loci and hila
(Heuchert et al. 2005).
Alieri et al. (1984) recorded C. spongiosum from Florida
on Cenchrus sp. and Ahmad (1969) listed it on Cenchrus
pennisetiformis from Pakistan. Furthermore, the species is
mentioned in literature on several hosts belonging to quite distinct
plant families. Zhang et al. (2003) recorded it on Amaryllis vittata,
Areca catechu, Cassia siamea, Chrysanthemum coronarium,
Coix lacryma-jobi, Freesia refracta and Platycodon grandilorus.
Panicum maximum (Zambia), Setaria chevalieri (Sierra Leone) and
Setaria pallidifusca (Zambia) are given as additional hosts in Lenné
(1990). Vittal & Dorai (1994/1995) reported C. spongiosum from
India as colonising leaves of Eucalyptus tereticornis, and Williams
& Liu (1976) cited Melinis minutilora as host species from Sabah,
Malaysia. In Haiti the species was recorded on Chaetochloa setosa
(Benjamin & Slot 1969). The later material could be checked and
proved to be the genuine C. spongiosum. However, most of these
records, which could not be checked, are probably misidentiied
and not identical with C. spongiosum. Rao (1988) described this
species on arecanut stem from India.
Bilgrami et al. (1991) listed this species from India as
hyperparasite on Acrosporium (= Oidium) dendrophthoae
(Erysiphales), and Mathur & Mukerji (1981) published two additional
Indian records on Phyllactinia dalbergiae and P. moricola [as P.
corylea], but these collections probably refer to the hyperparasitic
C. uredinicola (Heuchert et al. 2005).
147. Cladosporium stanhopeae Allesch., Hedwigia 34:
221. 1895. Figs 294, 295.
Holotype: Germany, München, botanical garden, on faded leaves of
Stanhopea sp. (Orchidaceae), Sep. 1894, Allescher (M-0057717).
Lit.: Saccardo (1895: xlviii; 1899: 1081), Lindau (1907: 817),
Schubert & Braun (2004: 311), Schubert (2005b: 138–140).
Ill.: Schubert & Braun (2004: 312, ig. 7), Schubert (2005b: 139, ig.
67, pl. 29, igs E–G).
www.studiesinmycology.org
Fig. 294. Cladosporium stanhopeae (M-0057717). Conidiophores and conidia in
vivo. Scale bar = 10 µm. K. Bensch del.
In vivo: On faded leaves, leaf spots amphigenous, varying
in shape and size, mostly irregular, medium to dark brown,
sometimes almost blackish, stromatic, occasionally somewhat
shiny. Colonies hypophyllous, small, punctiform, scattered,
greyish brown, not conined to leaf spots. Mycelium internal;
hyphae sparingly branched, 1.5–3 μm wide, septate, subhyaline
to pale yellowish brown, walls unthickened to slightly thickened.
Stromata dense, often large, up to 130 μm diam, composed
of subglobose to somewhat angular cells, 4–12 μm diam,
medium to dark brown, smooth, walls only slightly thickened.
Conidiophores solitary or in small, loose fascicles, arising from
stromata, erumpent through the cuticle, erect, usually more or
less lexuous, unbranched, up to 250 μm long, 3–5.5 μm wide,
septate, pale to medium brown, slightly paler towards the apex,
smooth, walls thickened, somewhat attenuated towards the apex,
without swellings. Conidiogenous cells integrated, terminal or
intercalary, 5–25 μm long, with a single or only few conidiogenous
loci, arranged on about the same level (like a garland), sometimes
situated on small lateral shoulders, loci 1–2 μm diam, thickened,
only slightly darkened-refractive. Conidia catenate, in branched
chains, subglobose, ovoid, obovoid, ellipsoid, subcylindrical to
cylindrical, 2–25 × 2–6 μm, 0–4(–5)-septate, mostly not constricted
at the septa, pale olivaceous to pale olivaceous-brown, smooth
or almost so to verruculose, walls more or less thickened, apex
rounded or with up to six apical hila, truncate to slightly convex
at the base, hila 0.5–2 μm diam, thickened, somewhat darkenedrefractive; occasionally with microcyclic conidiogenesis.
257
BenSch et al.
Fig. 295. Cladosporium stanhopeae (M-0057717). A. Symptoms. B. Overview. C. Conidiophore and conidia. Scale bars = 10 (C), 50 (B) µm.
Substrate and distribution: On Stanhopea; Germany.
Notes: Cladosporium herbarum, C. oxysporum and C.
sphaerospermum, widespread saprobic species occurring on a wide
range of substrates, are easily distinguishable from C. stanhopeae.
The conidia of C. oxysporum are smooth and only 0–1(–2)-septate,
the conidiophores are consistently nodulose. Cladosporium
herbarum differs in having conidiophores with intercalary and
terminal swellings, which are connected with conidiogenesis,
and verruculose to verrucose conidia (small subglobose conidia
absent). Cladosporium sphaerospermum forms small conidia,
which are globose to subglobose and distinctly verrucose, as well
as 0–3-septate, smooth or verruculose secondary ramoconidia,
and the conidiophores do not have any swellings.
Cladosporium tenuissimum is morphologically comparable with
C. stanhopeae but deviates in having longer and somewhat wider
conidiophores with often uni- or multilaterally swollen apices and
0–1-septate conidia.
148. Cladosporium subinlatum K. Schub., Zalar, Crous &
U. Braun, Stud. Mycol. 58: 143. 2007. Figs 296–298.
Holotype: Slovenia, Sečovlje, crystallization ponds, salterns,
isolated from hypersaline water, 2005, S. Sonjak (CBS H-19864).
Isotype: HAL 2027 F. Ex-type culture: CBS 121630 = CPC 12041
= EXF-343.
258
Fig. 296. Cladosporium subinlatum (CPC 12041). Macro- and micronematous
conidiophores and conidia in vitro. Scale bar = 10 µm. K. Bensch del.
Ill.: Schubert et al. (2007b: 143–144, Figs 37–39).
In vitro: Mycelium unbranched or occasionally branched, 1.5–3 μm
wide, later more frequently branched and wider, up to 7 μm wide,
the genuS Cladosporium
Fig. 297. Cladosporium subinlatum (CPC 12041). A–C. Macronematous conidiophores. D–E. Conidia. Scale bar = 10 µm.
Fig. 298. Cladosporium subinlatum
(CPC 12041). A–G. Images of an
11-d-old culture on SNA. A. Overview
of colony with clusters of conidia and
aerial hyphae. Many of the hyphae
have a collapsed appearance. B.
Detail of colony with conidiophores,
conidia and aerial hyphae that
are partly collapsed. C. Detail of a
conidiophore end and a secondary
ramoconidium. Note the scars at
the end of the conidiophore. D.
Details of conidia and ornamentation.
The ornamentation consists out of
markedly deined units, which have
a relatively large distance from
each other. Note the hilum on the
right conidium. E. Conidiophore with
large scars and conidia. F. Different
blastoconidia with very early stages
of new spore formation in the middle
of the picture. G. Pattern of spore
development. Scale bars = 2 (D), 5
(B, E–G), 10 (C), 20 (A) µm.
www.studiesinmycology.org
259
BenSch et al.
septate, not constricted at the septa, hyaline or subhyaline, almost
smooth to somewhat verruculose or irregularly rough-walled, walls
unthickened. Conidiophores mainly macronematous, sometimes
also micronematous, arising terminally from ascending hyphae or
laterally from plagiotropous hyphae, erect or subdecumbent, straight
or lexuous, sometimes bent, cylindrical, nodulose, usually with
small head-like swellings, sometimes swellings also on a lower level
or intercalary, occasionally geniculate, unbranched, occasionally
branched, (5–)10–270 × (1.5–)2.5–4.5(–5.5) μm, swellings 3–6.5 μm
wide, aseptate or with few septa, not constricted at the septa, pale
brown, pale olivaceous-brown or somewhat reddish brown, smooth,
usually verruculose or irregularly rough-walled and paler, subhyaline
towards the base, walls thickened, sometimes appearing even twolayered, up to 1 μm thick. Conidiogenous cells integrated, usually
terminal or conidiophores reduced to conidiogenous cells, cylindrical,
nodulose, usually with small head-like swellings with loci conined
to swellings, sometimes geniculate, 5–42 μm long, proliferation
sympodial, with several loci, up to four situated at nodules or on
lateral swellings, protuberant, conspicuous, denticulate, (0.8–)1–2
μm diam, thickened and darkened-refractive. Conidia catenate,
in branched chains, more or less straight, numerous globose and
subglobose conidia, ovoid, obovoid, broadly ellipsoid to cylindrical,
4–17(–22) × (2.5–)3.5–5.5(–7) μm [av. ± SD, 11.7 (± 4.6) × 4.5 (±
0.8) μm], 0–1(–2)-septate, not constricted at septa, pale brown or
pale olivaceous-brown, ornamentation variable, mainly densely
verruculose to echinulate (loosely muricate under SEM), spines up
to 0.8 μm high, sometimes irregularly verrucose with few scattered
tubercles or irregularly echinulate, walls unthickened or slightly
thickened, apex rounded or slightly attenuated towards apex and
base, hila conspicuous, protuberant, denticulate, 0.5–2 μm diam,
thickened and darkened-refractive; microcyclic conidiogenesis
observed.
Culture characteristics: Colonies on PDA attaining 29 mm diam after
14 d at 25 ºC, olivaceous-black to olivaceous-grey towards margin,
margin regular, entire edge, narrow, colourless to white, glabrous to
feathery, aerial mycelium formed, luffy, mainly near margins, growth
lat, somewhat folded in the colony centre, deep into the agar, few
prominent exudates formed with age, sporulation profuse. Colonies
on MEA attaining 25 mm diam after 14 d at 25 ºC, olivaceous-grey
to olivaceous due to abundant sporulation in the colony centre, pale
greenish grey towards margin, iron-grey reverse, velvety to powdery,
margin crenate, narrow, white, glabrous, radially furrowed, aerial
mycelium diffuse, growth convex with papillate surface, wrinkled
colony centre, without prominent exudates, sporulation profuse.
Colonies on OA attaining 26 mm diam after 14 d at 25 ºC, olivaceous,
iron-grey to greenish black reverse, growth lat, deep into the agar,
with a single exudate, abundantly sporulating.
Substrate and distribution: Hypersaline water; Slovenia.
Notes: Cladosporium subinlatum is a saprobic hyphomycete wellcharacterised by the formation of numerous globose or subglobose
conidia, resembling species of the C. sphaerospermum complex,
with coarse surface ornamentation ranging from verruculose to
distinctly spiny. Cladosporium spinulosum, also isolated from
hypersaline water, is morphologically close to C. subinlatum,
but differs from the latter species in having somewhat narrower
macronematous conidiophores, narrower conidiogenous loci and
hila, and conidia with longer spines, up to 1.3 μm. Cladosporium
allicinum may supericially also be confusable, but its conidia are
minutely verruculose to verrucose but never spiny.
260
Fig. 299. Cladosporium subobtectum (NY). Mycelium, conidiophores and conidia in
vivo. Scale bar = 10 µm. U. Braun del.
149. Cladosporium subobtectum U. Braun & K. Schub.,
Schlechtendalia 16: 71. 2007. Fig. 299.
Holotype: USA, California, Santa Clara Co., Stanford University,
on Euonymus sp. cult. (Celastraceae), 26 Oct. 1901, C.F. Baker,
Paciic Slope Fungi 31 (NY). Isotypes: Baker, Paciic Slope Fungi
31 (e.g., B 700006689).
Ill.: Braun & Schubert (2007: 72, ig. 6).
Exs.: Baker, Paciic Slope Fungi 31.
In vivo: Leaf blotches large, yellowish to whitish, straw-coloured, later
darkened, margin indeinite or with a narrow dark border. Colonies
amphigenous, subeffuse, dark brownish or olivaceous-brown,
velvety. Mycelium internal and external; supericial hyphae branched,
2–8 μm wide, septate, thin-walled (< 1 μm), subhyaline to pale
olivaceous-brown, often with swellings and constrictions. Stromata
lacking. Conidiophores solitary, arising from supericial hyphae,
lateral, occasionally terminal, erect, straight, subcylindrical-iliform
to somewhat geniculate-sinuous, unbranched, often narrowed and
paler towards the apex, 20–100 × 3–6 μm, septate, thin-walled (≤ 1
μm), pale olivaceous to medium olivaceous-brown or brown, almost
smooth to distinctly verruculose. Conidiogenous cells integrated,
terminal as well as intercalary, 10–25 μm long, proliferation sympodial,
occasionally percurrent, conidiogenous loci 1–2 μm diam, distinctly
coronate. Ramoconidia rarely formed. Conidia in simple or branched
the genuS Cladosporium
Fig. 301. Cladosporium subsclerotioideum (DAOM). A. Overview. B. Conidiophores.
C. Conidiophores and conidia. D. Conidiophores with coronate scars. E. Germinating
conidium. F, G. Conidia showing surface ornamentation. Scale bars = 10 (B–G), 50
(A) µm.
Fig. 300. Cladosporium subsclerotioideum (DAOM). Conidiophores and conidia in
vivo. Scale bar = 10 µm. K. Bensch del.
chains, subglobose, ellipsoid-ovoid, fusiform, subcylindrical, 5–28 ×
2.5–6(–8) μm 0–4-septate, pale olivaceous to medium olivaceousbrown, occasionally darker, thin-walled (≤ 1 μm), verruculose,
ends rounded to attenuated, hila 1–2 μm diam, often denticle-like;
microcyclic conidiogenesis not observed.
Substrate and distribution: On Euonymus sp.; USA.
Notes: Cladosporium subobtectum is a leaf-spotting hyphomycete,
which is morphologically close to C. obtectum (Schubert 2005b),
a species causing lesions on Artemisia maritima. However, the
latter species is distinguished from C. subobtectum by having
much shorter conidiophores, larger conidiogenous loci and conidial
hila and wider, often smooth conidia. The saprobic C. astroideum
is another similar species, which differs from C. subobtectum in
having much wider, (3–)4–11 μm, thick-walled (0.5–1.5 μm),
smooth to verruculose conidia, often with distinct lumina that render
them seemingly very thick-walled.
150. Cladosporium subsclerotioideum Bubák & Dearn.,
Hedwigia 58: 33. 1916. Figs 300, 301.
Lectotype (designated here): Canada, Ontario, London, on living
leaves of Turritis glabra (≡ Arabis glabra) (Brassicaceae), Jun.–
www.studiesinmycology.org
Jul. 1910, J. Dearness, mixed infection with Peronospora parasitica
and Albugo candida (BPI 427479). Isolectotype: DAOM.
Lit.: Saccardo (1931: 790), Schubert (2005b: 140–142).
Ill.: Schubert (2005b: 141, ig. 68, pl. 30, igs A–G).
In vivo: On still living or dying leaves and stems, without distinct leaf
spots or discolorations. Colonies amphigenous, at irst punctiform,
subcircular-oval, later conluent, caespitose, dense, dark brown,
somewhat raised, velvety. Mycelium internal, subcuticular to intraepidermal; hyphae sparingly branched, 3–7 μm wide, septate, with
swellings and constrictions, pale to medium olivaceous-brown or
even dark brown, smooth or almost so to somewhat rugose or
rough-walled, thick-walled, cells with oil droplets giving them a
granular appearance, forming dense, compact hyphal aggregations.
Conidiophores solitary or in loose groups, arising from stromatic
hyphal aggregations, erumpent through the cuticle, erect, straight
to more or less lexuous, sometimes slightly geniculate-sinuous,
subnodulose, but conidiogenous loci not conined to swellings,
unbranched or branched, 59–240 × 4–8.5(–10) μm, pluriseptate,
sometimes slightly constricted at the septa, olivaceous-brown, paler
towards the apex, tips sometimes subhyaline, smooth or almost
so to irregularly rugose or rough-walled, distinctly thick-walled,
often distinctly two-layered, with small to somewhat larger, shiny
or refractive oil droplets. Conidiogenous cells integrated, terminal
or intercalary, cylindrical, 15–50 μm long, proliferation sympodial,
with a single or only few conidiogenous loci, protuberant, slightly
convex, 1–2.5 μm diam, thickened, darkened-refractive. Conidia
261
BenSch et al.
catenate, in unbranched or rarely branched chains, straight, ovoid,
ellipsoid, fusiform, subcylindrical to cylindrical, 6–33(–44) × 4–8(–
9) μm, 0–4-septate, occasionally slightly constricted at the septa,
occasionally distoseptate, pale to medium olivaceous-brown,
surface ornamentation variable, smooth or almost so to irregularly
rugose or verruculose, thick-walled, sometimes even two-layered,
apex rounded or attenuated, often with small oil droplets, hila
protuberant, truncate to slightly convex, 1–2.5 μm diam, thickened,
darkened-refractive; often germinating, occasionally microcyclic
conidiogenesis occurring.
Substrate and distribution: On Turritis glabra; Canada.
Notes: On the label of the type collections the date is given as 1911,
whereas in the original diagnosis (Bubák 1916) 1910 is published.
This species is morphologically comparable with C. herbarum, but
the latter species is quite distinct in having subnodulose or nodulose
conidiophores with conidiogenous loci conined to swellings, cells
without oil droplets, and usually shorter, verruculose or sometimes
even verrucose, but not rugose conidia without oil droplets.
151. Cladosporium subtilissimum K. Schub., Dugan,
Crous & U. Braun, Stud. Mycol. 58: 146. 2007. Figs 302–304.
Holotype: USA, isolated from a grape berry, F.M. Dugan, wf 99-2-9
sci 1 (CBS H-19865). Isotype: HAL 2028 F. Ex-type culture: CBS
113754.
Ill.: Schubert et al. (2007b: 145–147, igs 40–42).
Exs.: ? Kabát & Bubák, Fungi Imperf. Exs. 242, p.p.
Fig. 302. Cladosporium subtilissimum (CBS 113754). Macro- and micronematous
conidiophores and conidia in vitro. Scale bar = 10 µm. K. Bensch del.
In vitro: Mycelium unbranched or sparingly branched, 1–5 μm
wide, septate, without swellings and constrictions, hyaline to
subhyaline or pale brown, smooth to minutely verruculose, walls
unthickened or almost so, protoplasm somewhat guttulate or
granular. Conidiophores macronematous and micronematous,
arising laterally from plagiotropous hyphae or terminally from
ascending hyphae, erect, straight to slightly lexuous, iliform to
cylindrical-oblong, non-nodulose, sometimes geniculate towards
Fig. 303. Cladosporium subtilissimum (CBS 113754). A–C. Macronematous conidiophores. D. Conidial chain. E. Micronematous conidiophore. F–G. Conidia. Scale bars = 10
µm.
262
the genuS Cladosporium
Fig. 304. Cladosporium subtilissimum (CBS 113754). A. Overview on the organisation of spore formation. The micrograph shows a large basal secondary ramoconidium which
has chains of secondary ramoconidia, intercalary and small terminal conidia. The conidia are formed in rows of often three cells. Note the size difference in the different cells.
B. Conidiophore showing very pronounced scars that almost appear as branches. C. Detail of (A), illustrating the scar formation between the cells. D. Conidia during different
stages of formation. E. Details of pronounced hila, and prominent ornamentation on secondary ramoconidia with the central dome-formed area. F. Different conidia and hila.
Scale bars = 2 (E), 5 (B–D, F), 10 (A) µm.
the apex, unbranched or once branched, branches short to
somewhat longer, usually formed below a septum, sometimes only
short, denticle-like or conical, 25–140 × 2–4 μm, 0–4-septate, not
constricted at the septa, subhyaline to pale brown, almost smooth,
minutely verruculose to verruculose, sometimes irregularly roughwalled in the lower part, walls unthickened or slightly thickened,
protoplasm guttulate or somewhat granular. Conidiogenous cells
integrated, terminal or pleurogenous, sometimes also intercalary,
iliform to narrowly cylindrical, non-nodulose, sometimes
geniculate, 14–57 μm long, with usually sympodial clusters of
pronounced conidiogenous loci at the apex or on a lower level,
denticle-like or situated on short lateral prolongations, up to ive
loci, intercalary conidiogenous cells usually with a short denticlelike lateral outgrowth below a septum, protuberant, denticulate,
somewhat truncate, 1.2–2 μm diam, thickened and darkenedrefractive. Ramoconidia sometimes occurring, conidiogenous
cells seceding at one of the upper septa of the conidiophore and
www.studiesinmycology.org
behaving like conidia, iliform or cylindrical, 20–40(–55) μm long,
1.5–4 μm wide, 0–1-septate, concolorous with conidiophores, not
attenuated towards apex and base, base broadly truncate, noncladosporioid, without any dome and raised rim, 2–3.5 μm wide,
neither thickened nor darkened, sometimes slightly refractive.
Conidia catenate, in branched chains, up to 12 or even more in
a chain, straight, small terminal conidia numerous, subglobose,
narrowly obovoid, limoniform or fusiform, 4–9 × 2–3.5 μm [av. ±
SD, 6.4 (± 1.5) × 2.8 (± 0.4) μm], with up to three distal scars,
aseptate, hila (0.5–)0.8–1 μm diam, intercalary conidia narrowly
ellipsoid, fusiform to subcylindrical, 9–18 × 3–4(–6) μm [av. ± SD,
13.0 (± 2.5) × 3.8 (± 0.3) μm], 0(–1)-septate, hila 1–1.2(–1.8) μm
diam, with up to four distal scars, secondary ramoconidia ellipsoid,
fusiform or subcylindrical, (13–)17–32(–37) × 3–5(–6) μm [av. ±
SD, 21.4 (± 4.4) × 4.1 (± 0.5) μm], 0–1(–2)-septate, septum median
or somewhat in the lower half, usually not constricted at the septa,
with up to six distal hila crowded at the apex, hila (1.2–)1.5–2(–2.5)
263
BenSch et al.
μm diam, apex often somewhat laterally enlarged or prolonged with
hila crowded there, very pale or pale brown or olivaceous-brown,
minutely verruculose to verruculose (granulate under SEM), walls
unthickened or only slightly thickened, often slightly attenuated
towards apex and base, protoplasm often guttulate or granular,
hila protuberant, denticulate, (0.5–)0.8–2(–2.2) μm diam, thickened
and darkened-refractive; microcyclic conidiogenesis occasionally
observed.
Culture characteristics: Colonies on PDA attaining 24 mm diam
after 14 d at 25 °C, grey-olivaceous to olivaceous, olivaceousgrey, iron-grey or olivaceous-black reverse, velvety, margin
regular, entire edge, white or pale greenish olivaceous, glabrous
to feathery, aerial mycelium sparse, only few areas with abundant
mycelium, diffuse, growth regular, lat or with a raised and wrinkled
colony centre, radially furrowed, effuse, usually without prominent
exudates, with age several exudates formed, sporulation profuse,
colonies consisting of two kinds of conidiophores, short and a few
longer ones. Colonies on MEA reaching 25 mm diam after 14 d
at 25 °C, greenish olivaceous to grey-olivaceous in the centre,
olivaceous-grey to iron-grey reverse, velvety, margin entire
edge, crenate or umbonate, narrow, pale greenish olivaceous,
sometimes radially furrowed, aerial mycelium absent or sparse,
growth low convex with distinctly wrinkled colony centre, without
prominent exudates, abundantly sporulating. Colonies on OA
attaining 25 mm diam after 14 d at 25 °C, dark grey-olivaceous
to olivaceous due to profuse sporulation, iron-grey reverse,
sometimes releasing some olivaceous-buff pigments into the
agar, velvety, margin regular, entire edge or crenate, narrow,
colourless or white, glabrous or feathery, aerial mycelium sparse,
growth lat with slightly raised colony centre, prominent exudates
lacking, sporulation profuse.
Substrate and distribution: Plant material and hypersaline water;
Europe (Slovenia), North America (USA).
Additional specimens examined: Slovenia, Sečovlje, isolated from hypersaline
water from salterns (reserve pond), 2005, P. Zalar (CPC 12044 = EXF-462). USA,
isolated from bing cherry fruits, F.M. Dugan (CBS 113753).
Excluded strains within the subtilissimum complex: Argentina, isolated from Pinus
ponderosa (Pinaceae), 2005, A. Greslebin (CPC 12484, CPC 12485). USA, isolated
from grape berry, F.M. Dugan (CBS 113741, CBS 113742); isolated from grape bud,
F.M. Dugan (CBS 113744).
Notes: Cladosporium subtilissimum is a species of the C. herbarum
complex but morphologically resembles C. cladosporioides. It
deviates from C. cladosporioides s. str. by having verruculose to
verrucose conidia. Schubert et al. (2007b) listed and discussed
several isolates which are in vitro morphologically not distinguished
from C. subtilissimum but genetically separated in various
subclades, so that they can only be referred to as Cladosporium
sp. or C. subtilissimum s. lat. used as morphological species.There
are similar problems with regard to the identiication of herbarium
specimens that morphologically agree with C. subtilissimum
[e.g., Spain, Ceuda, on dead leaves of Eucalyptus viminalis, 26
Dec. 1925, Vidal y Lopes 7602 (MA 07502); Germany, Berlin,
Grunewaldsee, on Typha angustifolia, 9 Oct. 1904, G. Lindau,
Kabát & Bubák, Fungi Imperf. Exs. 242 (B 700006756); France,
Loire, Veauches, on T. latifolia, Aug. 1883, Hernier, ex herb. G.
Winter (B 700006750)].
264
Fig. 305. Cladosporium subuliforme (CBS 126500). Subulate conidiophores,
ramoconidia and conidial chains in vitro. Scale bar = 10 µm. K. Bensch del.
152. Cladosporium subuliforme Bensch, Crous & U. Braun,
Stud. Mycol. 67: 77. 2010. Figs 305, 306.
Holotype: Thailand, Chiang Mai, Sansai, Mai Jo, palm nursery,
isol. from Chamaedorea metallica (Arecaceae), 26 Dec. 2006, coll.
I. Hidayat & J. Meeboon, FIH 401, isol. P.W. Crous (CBS H-20448).
Ex-type culture: CBS 126500 = CPC 13735.
Ill.: Bensch et al. (2010: 78, igs 67−68).
In vitro: Mycelium internal and supericial; hyphae sparingly
branched, 1−4 μm wide, septate, sometimes slightly constricted at
the base of conidiophores, subhyaline to pale olivaceous-brown,
smooth to minutely verruculose or verruculose, often somewhat
swollen at the base of conidiophores, up to 6 μm wide, sometimes
forming ropes. Conidiophores macro- to semimacronematous or
micronematous, solitary or in pairs, arising terminally and laterally
from hyphae, erect, straight to mostly lexuous, iliform to narrowly
cylindrical-oblong, often slightly to distinctly attenuated towards
the apex and wider at the base, not nodulose or geniculate,
unbranched or branched, branches often only as short peg-like
lateral outgrowth just below a septum bearing conidiogenous loci,
branches occasionally longer, up to 20 μm, 9−330 × (1.5−)2−3(−3.5)
μm, pluriseptate, usually not constricted at septa, pale to medium
olivaceous-brown, smooth to sometimes minutely verruculose,
parts of the stalk occasionally verrucose, basal cell sometimes
swollen up to 8(−10) μm, walls unthickened or only slightly
thickened, about 0.5 μm wide. Conidiogenous cells integrated,
mainly terminal but also intercalary, narrowly cylindrical-oblong,
the genuS Cladosporium
Fig. 306. Cladosporium subuliforme (CBS 126500). A–C. Tips of conidiophores with conidial chains. D. Subulate conidiophore with terminal and intercalary conidiogenous cell
and conidia. Scale bar = 10 µm.
neither nodulose nor geniculate, 9−40 μm long, with up to ive
loci crowded at the uppermost apex, in intercalary cells loci often
situated on small denticle- or peg-like lateral outgrowths just below
a septum, loci conspicuous, subdenticulate, (0.8−)1−1.5(−1.8) μm
diam, thickened and darkened-refractive. Ramoconidia commonly
formed, cylindrical-oblong, differentiation between ramoconidia
and secondary ramoconidia often quite dificult, (14−)17−35 ×
(1.5−)2−3 μm, 0(−1)-septate, pale olivaceous-brown, smooth,
walls unthickened, not attenuated towards the base, base broadly
truncate, 2−2.5 μm wide, unthickened, but often somewhat
darkened or refractive. Conidia numerous, catenate, in branched
chains, up to 5−6 conidia in the unbranched terminal part of the
chain, branching in all directions, straight, small terminal conidia
obovoid, subglobose, ovoid to limoniform or ellipsoid, 2.5−4.5(−5.5)
× 2−2.5 μm (av. ± SD: 4.2 ± 0.9 × 2.2 ± 0.2), aseptate, rounded at
the apex, attenuated towards the base, intercalary conidia ellipsoid
to subcylindrical, 5.5−12(−13) × 2−3(−3.5) μm (av. ± SD: 8.3 ± 2.5
× 2.6 ± 0.4), aseptate, with up to four distal hila, attenuated towards
apex and base, secondary ramoconidia ellipsoid to subcylindrical,
sometimes cylindrical-oblong, (6−)8−25(−28) × 2−3(−3.5) μm (av.
± SD: 15.1 ± 7.3 × 2.7 ± 0.4), 0−1-septate, not constricted at
septa, median or somewhat in the lower half, usually somewhat
attenuated towards the base, (2−)3−4(−5) distal hila, pale
olivaceous-brown, smooth or almost so, walls unthickened, hila
conspicuous, subdenticulate to denticulate, (0.2−)0.5−1.5(−1.8) μm
diam, somewhat thickened and darkened-refractive; microcyclic
conidiogenesis not observed.
Culture characteristics: Colonies on PDA grey-olivaceous to mainly
olivaceous-grey, reverse olivaceous-grey, velvety to loccose,
luffy, margins grey-olivaceous to white, feathery, regular or slightly
undulate, aerial mycelium abundant, loose, luffy, growth effuse to
low convex, without exudates, sporulation profuse. Colonies on
MEA greenish olivaceous to pale olivaceous-grey and olivaceousbuff, glaucous-grey at margins, reverse olivaceous-grey, loccose
to luffy, margins white, glabrous, regular to somewhat undulate,
radially furrowed and wrinkled, effuse, aerial mycelium abundant,
luffy, mainly in colony centre, without exudates, sporulation profuse.
Colonies on OA whitish to smoke-grey and pale olivaceous-grey,
www.studiesinmycology.org
olivaceous-buff and dull green towards margins, somewhat zonate,
grey-olivaceous due to sporulation, reverse leaden-grey, loccose
to felty, margins dull green or colourless, regular, glabrous, aerial
mycelium abundant, loccose to luffy-felty, covering large parts of
colony surface, growth effuse, without exudates, sporulating.
Substrate and distribution: Isolated from Chamaedorea metallica;
Asia (Thailand).
Notes: Cladosporium subuliforme belongs to the C. cladosporioides
complex, but deviates from allied species, above all C.
cladosporioides and C. tenuissimum, by its long narrow subulate
conidiophores with several loci crowded at the apex and its
numerous ramoconidia with narrow loci and hila. Cladosporium
angustisporum is phylogenetically close to this species (see Bensch
et al. 2010, ig. 1, part c) but morphologically easily separable. The
conidiophores are not subuliform and the terminal conidia are
somewhat longer and narrower.
153. Cladosporium syringicola K. Schub. & U. Braun,
Schlechtendalia 14: 80. 2006. Figs 307, 308.
Holotype: Germany, Sachsen-Anhalt, Halle (Saale), botanical
garden, on Syringa ×chinensis (Oleaceae), 2 Aug. 2004, K.
Schubert, mixed infection with Erysiphe syringae-japonicae (U.
Braun) U. Braun & S. Takam. (HAL 1835 F). Isotypes: Braun, Fungi
Sel. Exs. 52 (BPI, GZU, HMAS, IMI, KR, KUS, LE, M, PDD, VPIR).
Lit.: Schubert (2005b: 142–144).
Ill.: Schubert (2005b: 143, ig. 69, pl. 31, igs A–G), Schubert et al.
(2006: 81, ig. 12, pl. 2, igs I–J).
In vivo: On living leaves, leaf spots amphigenous, distinct, at irst
punctiform, later extending, usually irregular in shape, 1–25 mm
wide, pale to medium brown, occasionally somewhat zonate,
surrounded by a narrow, dark brown margin, sometimes with a pale
greenish halo, on the lower leaf surface somewhat paler, conluent.
Colonies amphigenous, loosely scattered, in small tufts, dark brown
265
BenSch et al.
Fig. 307. Cladosporium syringicola (HAL 1835 F). Symptoms, dimorphic
conidiophores and conidia in vivo. Scale bar = 10 µm. K. Bensch del.
to blackish, tips of sporulating conidiophores pale olivaceous.
Mycelium internal and external, hyphae emerging through stomata
and then growing supericially; hyphae loosely branched, 2–5 μm
wide, septate, sometimes constricted at the septa, often with small
swellings, subhyaline to pale olivaceous, smooth, walls thickened,
cells occasionally with distinct, clearly delineated, somewhat granular
lumen, forming stromatic hyphal aggregations. Stromatic hyphal
aggregations absent to well-developed, composed of swollen hyphal
cells, subcircular to somewhat angular, 6–15 μm wide, medium
to dark olivaceous-brown, smooth, thick-walled. Conidiophores
dimorphic, solitary or in small fascicles, usually emerging through
stomata or erumpent through the cuticle, arising from swollen hyphal
cells or stromatic hyphal aggregations, erect, straight to lexuous,
unbranched or often branched, not to somewhat geniculate-sinuous,
sometimes subnodulose, subcylindrical, attenuated towards the
apex, 25–140 × 4–8 μm, pluriseptate, occasionally constricted at the
septa, medium to dark olivaceous-brown or brown, smooth, walls
thickened, often distinctly two-layered, up to 1(–1.25) μm thick, cells
often with distinct, clearly delineated lumen, often swollen at the
base, up to 14 μm wide; conidiophores of the second type shorter,
narrower and paler, solitary, arising from supericial hyphae, erect,
more or less straight, somewhat geniculate, unbranched, 9–35
× 2–4 μm, septate, not constricted at the septa, pale olivaceous,
smooth, walls thickened, not distinctly two-layered, but sometimes
with distinct, delineated lumen, slightly attenuated towards the apex,
often somewhat swollen at the base. Conidiogenous cells integrated,
terminal or intercalary, 7–25 μm long, proliferating sympodially,
with numerous, subdenticulate conidiogenous loci, often crowded,
apex appearing somewhat rugose (periconiella-like), 0.5–2 μm
diam, somewhat thickened and darkened-refractive. Ramoconidia
266
Fig. 308. Cladosporium syringicola (HAL 1835 F). A. Symptoms. B. Overview. C.
Fascicle of conidiophores emerging through stomata. D. Tip of a conidiophore with
numerous, conspicuous, crowded, coronate conidiogenous loci and conidia still
attached. E, G. Dimorphic conidiophores, second type of conidiophores arising from
external, creeping hyphae. F. Tip of a conidiophore with conidia still attached. Scale
bars = 10 (C–F), 20 (B) µm.
rarely occurring. Conidia catenate, in branched chains, straight to
slightly curved, subglobose, obovoid, ellipsoid, subcylindrical to
irregular, 2.5–18 × 2–5(–6.5) μm, 0–2(–3)-septate, not constricted
at the septa, subhyaline, pale olivaceous to pale olivaceous-brown,
smooth to sometimes minutely verruculose, walls thickened, often
with distinct, clearly delineated lumen, apex rounded to somewhat
attenuated, with numerous hila, 0.5–2 μm diam, somewhat thickened
and darkened-refractive; microcyclic conidiogenesis often occurring.
Substrate and distribution: On Syringa ×chinensis; Germany.
Additional specimen examined: Germany, Saxony, Dresden, Leubnitz-Neuostra, on
Syringa vulgaris, 12 Oct. 2010, E.G. Mahn (HAL 2421 F).
Notes: Based on frequently branched conidiophores and
conidiogenous cells with numerous crowded conidiogenous
loci, Cladosporium syringicola is morphologically close to and
comparable with C. gallicola, but the latter species differs in
having uniform, non-dimorphic conidiophores, somewhat wider
conidiogenous loci (1–3 μm), and somewhat longer and wider
conidia, 3–29 × 2–8 μm, with 0–3(–4) septa (Heuchert et al. 2005).
Dimorphic conidiophores are also formed in C. fusicladiiformis, a
leaf-spotting species known from Spain on Eriobotrya japonica,
but the latter species is quite distinct in having much longer
conidiophores, up to 300 μm, and usually 0–1-septate conidia
without a distinct, clearly delineated lumen.
the genuS Cladosporium
Cladosporium fumago var. maculaeforme (type: on Syringa
vulgaris, Thüm., Mycoth. Univ. 673 M-0057646, examined) does
not belong in Cladosporium s. str., since the conidiogenous loci
and hila are non-cladosporioid; the generic afinity is not yet
clear. Cladosporium fumago f. syringae-vulgaris (type: on Syringa
vulgaris, Thüm., Herb. Mycol. Oecon. 393, M-0057668, examined)
is a Trimmatostroma-like hyphomycete, which has to be excluded
from Cladosporium s. str. Cladosporium syringae (type material
not traced) was described to have fasciculate, unbranched
conidiophores, 3–4 μm wide, and cylindrical conidia, 15–20 μm
long (Saccardo 1931).
154. Cladosporium taphrinae Bubák, Bot. Közlem. 15(3–
4): 81. 1915. Figs 309, 310.
Holotype: Montenegro, Šavnik, on Taphrina coerulescens
(Taphrinales) on Quercus cerris (Fagaceae), 30 Sep. 1911, L.
Vlach (BPI 427506).
Lit.: Saccardo (1931: 797), Heuchert et al. (2005: 40).
Ill.: Heuchert et al. (2005: 41, ig. 15, pl. 2, igs 10–11).
In vivo: Colonies olivaceous to dark brown, loose to dense,
subcircular in outline, later extended, conluent, caespitose,
velvety. Mycelium on, in and between asci; hyphae strongly
branched, 2–6 μm wide, septate, usually not constricted at the
septa, subhyaline, pale olivaceous to medium brown, forming
dense hyphal nets, cells sometimes swollen, 4–10 μm diam,
occasionally forming small stromata, pale brown, smooth, walls
thickened. Conidiophores solitary, arising from hyphae, or in
loose fascicles, arising from stromata, erumpent through asci,
erect, straight to curved, subcylindrical, rarely slightly geniculatesinuous, usually unbranched, rarely branched, 23–150(–200) ×
3–5(–7) μm, occasionally longer and up to 8 μm wide, 1–7-septate,
without any constrictions, medium brown, paler towards the apex,
tips often subhyaline, smooth or almost so, faintly rough-walled
at the base, walls somewhat thickened, but thinner towards the
apex, occasionally enterogenously proliferating and monopodial.
Conidiogenous cells integrated, terminal and intercalary, 9–48
μm long, polyblastic, proliferation sympodial, near the apex with
numerous conspicuous conidiogenous loci (up to 14), numerous
densely arranged loci often giving an impression to be slightly
inlated and denticulate, conidiogenous loci protuberant, denticlelike, 1–1.5(–2) μm diam, thickened and darkened. Conidia mostly
in branched chains, straight, subglobose, obovoid, ellipsoid, 4–20
× 2.5–6 μm, 0–2(–3)-septate, without any constrictions at the
septa, pale olivaceous to olivaceous-brown, smooth or almost so,
occasionally subechinulate, walls slightly thickened, small conidia
Fig. 309. Cladosporium taphrinae (BPI 427506). Conidiophores arising from
stromata and conidiophores arising from hyphae, erumpent through asci and
conidia in vivo. Scale bar = 10 µm. B. Heuchert del.
usually thin-walled, apex rounded to slightly attenuated, with up to
4 hila, base truncate to convex, occasionally somewhat attenuated,
hila protuberant, 0.5–1.5(–2) μm diam, thickened and darkened,
basal hilum of branched conidia convex, without a cladosporioid
hilum, up to 2 μm wide; microcyclic conidiogenesis not observed.
Substrate and distribution: On Taphrina spp. (Taphrinales);
Europe, North America – Taphrina coerulescens on Quercus cerris
(Montenegro), T. coerulescens (USA, NV).
Additional specimen examined: USA, NV, Lincoln County, dirt road toward Highland
Peak 5.6 miles from junction with Route 93, west of Pioche, 37°54'30" N 114°33'12"
W, on Taphrina coerulescens, 17 Aug. 1993, C.T. Rogerson (Fungi of Nevada) (NY,
as C. exoasci).
Notes: In contrast to Cladosporium phyllophilum (= C. exoasci),
the second Cladosporium species on Taphrina spp., C. taphrinae
grows on and between asci of Taphrina coerulescens. The
conidiophores arise from hyphae, are minutely verruculose at the
base and the tips are often somewhat swollen and appear coarsely
Fig. 310. Cladosporium taphrinae (BPI 427506). A, D. Overview showing conidiophores erumpent through asci and conidia. B. Conidiophores and conidia. C. Details of the
coronate scar structure on conidiophores and conidia. D. Scale bars = 5 (C), 10 (B), 20 (A, D) µm.
www.studiesinmycology.org
267
BenSch et al.
verrucose by densely arranged denticle-like scars. Stromata are
usually lacking, and the conidiogenous loci and conidial hila are
smaller. The conidia are uniform, without obvious dimorphism.
A collection on Taphrina coerulescens deposited at NY could be
identiied as C. taphrinae and represents the irst record for North
America (Heuchert et al. 2005).
155. Cladosporium tenellum K. Schub., Zalar, Crous & U.
Braun, Stud. Mycol. 58: 149. 2007. Figs 311–313.
Holotype: Israel, Ein Bokek, Dead Sea, isolated from hypersaline
water, 2004, M. Ota (CBS H-19866). Isotype: HAL 2029 F. Ex-type
culture: CBS 121634 = CPC 12053 = EXF-1735.
Ill.: Schubert et al. (2007b: 148–149, igs 43–45).
In vitro: Mycelium sparingly branched, 1–3 μm wide, septate,
septa often not very conspicuous, not constricted at the septa,
sometimes slightly swollen, subhyaline, smooth, walls unthickened.
Conidiophores macronematous and micronematous, solitary,
arising terminally or laterally from plagiotropous or ascending
hyphae, erect or subdecumbent, almost straight to more or less
lexuous, cylindrical, sometimes geniculate towards the apex, but
not nodulose, sometimes with short lateral prolongations at the
apex, unbranched to once or twice branched (angle usually 30–45°
degree, sometimes up to 90°), branches usually below a septum,
6–200 × (1–)2–4(–5) μm, septate, septa not very conspicuous,
not constricted at the septa, subhyaline to pale brown, almost
smooth to usually asperulate, walls unthickened or almost so.
Conidiogenous cells integrated, terminal or intercalary, sometimes
conidiophores reduced to conidiogenous cells, cylindrical,
sometimes geniculate, non-nodulose, 6–40 μm long, proliferation
sympodial, with several conidiogenous loci often crowded at the
apex and sometimes also at a lower level, situated on small lateral
shoulders, unilateral swellings or prolongations, with up to 6(–10)
Fig. 311. Cladosporium tenellum (CPC 12053). Macro- and micronematous
conidiophores and conidia in vitro. Scale bar = 10 µm. K. Bensch del.
denticulate loci, forming sympodial clusters of pronounced scars,
intercalar conidiogenous cells with short or somewhat long lateral
outgrowths, short denticle-like or long branches with several scars
at the apex, usually below a septum, loci protuberant, 1–1.5(–2) μm
diam, thickened and darkened-refractive. Ramoconidia sometimes
occurring, cylindrical, up to 32 μm long, 2.5–4 μm wide, with a
broadly truncate, unthickened base, about 2 μm wide. Conidia
catenate, formed in branched chains, straight, small terminal
conidia globose, subglobose, ovoid, oval, 3–6 × 2.5–3.5 μm [av.
± SD, 4.5 (± 1.3) × 2.8 (± 0.4) μm], aseptate, asperulate, with 0–2
distal hila, intercalary conidia and secondary ramoconidia ellipsoidovoid, ellipsoid to subcylindrical, 3.5–20(–28) × (2.5–)3–5(–6) μm
[av. ± SD, 12.4 (± 5.4) × 4.1 (± 0.7) μm], 0–1-septate, rarely with
up to three septa, sometimes slightly constricted at the septa,
Fig. 312. Cladosporium tenellum (CPC 12053). A–C, E. Macronematous conidiophore. D. Micronematous conidiophore. F. Ramoconidium and conidia. Scale bars = 10 µm.
268
the genuS Cladosporium
regular, entire edge, narrow, colourless to white, aerial mycelium
absent or sparingly formed, felty, whitish, growth regular, lat,
radially furrowed, with folded and elevated colony centre, deep into
the agar, with age forming few to numerous prominent exudates,
sporulation profuse, few high conidiophores formed. Colonies on
MEA reaching 25–44 mm diam after 14 d at 25 ºC, olivaceous-grey
to olivaceous- or iron-grey due to abundant sporulation in the colony
centre, velvety, margin regular, entire edge, narrow, colourless,
white to pale olivaceous-grey, aerial mycelium loose, diffuse,
growth convex with papillate surface, radially furrowed, wrinkled,
without prominent exudates, sporulating. Colonies on OA reaching
23–32 mm diam after 14 d at 25 ºC, grey-olivaceous, olivaceousgrey to olivaceous due to abundant sporulation in the colony centre,
olivaceous- or iron-grey reverse, velvety, margin regular, entire
edge, narrow, colourless or white, aerial mycelium sparse, diffuse,
loccose, growth lat to low convex, radially furrowed, wrinkled,
without prominent exudates, sporulation profuse.
Substrate and distribution: Hypersaline water and plant material;
Middle East (Israel), North America (USA).
Additional specimens examined: Israel, Eilat, isolated from hypersaline water from
salterns, 2004, N. Gunde-Cimerman (CBS 121633 = CPC 12051 = EXF-1083).
USA, Seattle, University of Washington campus, isolated from Phyllactinia sp.
(Erysiphaceae) on leaves of Corylus sp. (Corylaceae), 16 Sep. 2004, D. Glawe
(CPC 11813).
Fig. 313. Cladosporium tenellum (CPC 12053). A. A bird’s eye view of a colony of
C. tenellum with its very characteristic bundles of aerial hyphae. Numerous conidia
are visible, formed on simple conidiophores. B. Hyphae that run on the agar surface
give rise to conidiophores and numerous conidia that are relatively rounded. C.
Conidiophore ends are rather simple and have large scars. D. Hila on a secondary
ramoconidium with non-ornamented area. E. Detail of the prominent ornamentation
on a secondary ramoconidium. Scale bars = 2 (C, E), 5 (D), 10 (B), 20 (A) µm.
Notes: Cladosporium tenellum comprises characters of various
species complexes of the genus Cladosporium. The formation of
globose or subglobose terminal conidia is reminiscent of species in
the C. sphaerospermum complex. Based on the general morphology
and size of conidiophores and conidia C. tenellum is rather
comparable with species of the C. cladosporioides complex, e.g. C.
cladosporioides s. str. characterised by smooth conidiophores and
conidia with only few conidiogenous loci and conidial hila crowded
at the apex and somewhat wider conidiophores, 3–5(–6) μm.
However, it belongs to the C. herbarum complex where it resembles
C. subtilissimum and C. ramotenellum (Schubert et al. 2007b). In C.
subtilissimum the small terminal conidia are not globose but rather
narrowly obovoid to limoniform, the conidiogenous loci and conidial
hila are somewhat wider, (0.5–)0.8–2(–2.2) μm, and at the apices of
conidiophores and conidia only few scars are formed. Cladosporium
ramotenellum possesses longer and narrower, 0–3-septate conidia,
2.5–35 × 2–4(–5) μm, but forms only few conidiogenous loci and
conidial hila at the apices of conidiophores and conidia.
156. Cladosporium tenuissimum Cooke, Grevillea 6(40):
140. 1878. Figs 314–316.
subhyaline, pale brown to medium olivaceous-brown, asperulate
or verruculose (muricate, granulate or colliculate under SEM),
walls unthickened or slightly thickened, apex rounded or slightly
to distinctly attenuated towards apex and base, often forming
several apical hila, up to 7(–9), crowded, situated on small lateral
outgrowths giving them a somewhat irregular appearance, hila
protuberant, 0.5–1.5 μm diam, thickened and darkened-refractive;
microcyclic conidiogenesis sometimes occurring.
Lectotype (designated by Heuchert et al. 2005): USA, South
Carolina, Aiken, on leaf sheets of Zea mays (Poaceae), H.W.
Ravenel, Ravenel, Fungi Amer. Exs. 160 (NY). Isolectotypes:
Ravenel, Fungi Amer. Exs. 160 (e.g., K, PH 01020427). Topotype
material: Roumeguère, Fungi Sel. Gall. Exs. 5295 (e.g., NY).
Epitype (designated by Bensch et al. 2010): USA, Louisiana, Baton
Rouge, isol. from fruits of Lagerstroemia sp. (Lythraceae), 8 Sep.
2007, P.W. Crous (CBS H-20449). Ex-type culture: CBS 125995 =
CPC 14253
Culture characteristics: Colonies on PDA reaching 27–34 mm diam
after 14 d at 25 ºC, smoke-grey, grey-olivaceous to olivaceous-grey,
olivaceous-grey to iron-grey reverse, velvety to powdery, margin
= Cladosporium herbarum var. citricola H.S. Fawc. & O.F. Burger,
Phytopathology 1: 165. 1911 [lectotype, selected here: USA, Florida, Bayview,
26 Jul. 1909, H.S. Fawcett, FLAS, F 13144].
www.studiesinmycology.org
269
BenSch et al.
Fig. 314. Cladosporium tenuissimum (NY). Conidiophores emerging through
stomata, tips of conidiophores and conidia in vivo. Scale bar = 10 µm. B. Heuchert
del.
Lit.: Saccardo (1886: 365), Ellis (1976: 326), Ho et al. (1999: 140),
Zhang et al. (2003: 166–168), Heuchert et al. (2005: 50–52),
Bensch et al. (2010: 78–81).
Ill.: Ellis (1976: 327, ig. 245 A), Ho et al. (1999: 143, igs 46–47),
Zhang et al. (2003: 166, ig. 115), Heuchert et al. (2005: 51, ig. 20),
Bensch et al. (2010: 80–81, igs 69–70).
Exs.: Ellis, N. Amer. Fungi 651; Ravenel, Fungi Amer. Exs. 160;
Roumeguère, Fungi Sel. Gall. Exs. 5295.
In vivo: Colonies greyish brown to dark brown, effuse, conluent,
thickly felted to villose. Mycelium immersed; hyphae branched,
2–7 μm wide, septate, with constrictions at the septa, hyphal
cells sometimes irregularly swollen, sometimes irregularly lobed,
subhyaline to pale olivaceous-brown, hyphae giving rise to
conidiophores often darker, medium to dark brown, and walls
somewhat thickened. Stromata lacking. Conidiophores solitary or
in loose groups, mostly two or three, but not distinctly fasciculate,
arising from hyphae or somewhat swollen hyphal cells, on leaves
and stems erumpent through the cuticle or emerging through
stomata, erect, straight to slightly curved-sinuous, but not
geniculate-sinuous, subcylindrical-iliform, unbranched, 50–550(–
800) × 3–7(–8) μm, at the very base sometimes wider, somewhat
270
Fig. 315. Cladosporium tenuissimum (CBS 125995). Macro- and micronematous
conidiophores usually with a head-like swollen apex and sometimes additional
intercalary nodules, conidial chains and microcyclic conidiogenesis in vitro. Scale
bar = 10 µm. K. Bensch del.
inlated, often somewhat attenuated towards the tip, pluriseptate
throughout, usually 3–18-septate, non-constricted at the septa,
pale, medium to dark brown or olivaceous-brown, often paler
towards the tip, smooth, occasionally faintly rough-walled, wall
somewhat thickened, up to 1.5 μm, but tips usually unthickened,
cells occasionally with distinct lumen, giving the impression that the
walls are two-layered and thick, up to 2 μm wide, occasionally with
unilateral slight swellings. Conidiogenous cells integrated, terminal,
sometimes intercalary, cylindrical to subclavate, 15–40 μm long,
occasionally longer, polyblastic, with 2–5 coronate conidiogenous
loci, sympodially proliferating, terminal conidiogenous cells
unswollen, tips subdenticulate, subgeniculate, slightly curved or
often sligthly swollen, multilateral or often only unilateral, with a single
or several conspicuous conidiogenous loci, somewhat protuberant,
thickened and darkened, 1–2.5 μm diam. Ramoconidia abundant,
with a truncate or obconically truncate base, 1.5–2.5 μm wide, up
to 30 × 7 μm, (0–)1(–3)-septate. Conidia in branched chains, small
terminal and intercalary conidia variable, subglobose, ellipsoidovoid, limoniform, 3–10(–13) × 2–5 μm, 0(–1)-septate, secondary
ramoconidia subcylindrical, ellipsoid-ovoid, fusiform, 8–25 × 3–6
μm, 0–1-septate, slightly or not constricted at the septa, pale
olivaceous to olivaceous-brown, smooth to rough-walled (smooth
and verruculose conidia usually mixed), wall unthickened to slightly
the genuS Cladosporium
Fig. 316. Cladosporium tenuissimum (CBS 125995). A–E, G–H. Macronematous conidiophores and conidial chains. F, I–J. Micronematous conidiophores with conidia. Scale
bars = 10 µm.
thickened (0.5–1 μm), apex rounded or somewhat irregular by
having up to four protuberant hila, base truncate to convex or often
somewhat attenuated, hila thickened, darkened, 0.5–1.5(–2) μm
diam; microcyclic conidiogenesis not observed.
In vitro: Mycelium immersed and supericial, hyphae branched,
(0.5−)1−5 μm wide, septate, sometimes constricted at septa,
subhyaline to pale or medium brown, with swellings and
constrictions, often irregular in outline, smooth to sometimes
minutely verruculose, sometimes appearing rough-walled, walls
unthickened or very slightly thickened, sometimes forming ropes.
Conidiophores solitary, macronematous and micronematous,
arising terminally and laterally from hyphae; macronematous
conidiophores solitary, sometimes in groups of 2−3, erect,
straight or slightly lexuous, cylindrical-oblong to almost iliform,
sometimes slightly to distinctly geniculate towards the apex,
often subnodulose or nodulose with an apical and sometimes a
few additional swellings on a lower level, swellings quite distant
from the apex and from each other, most conidiophores neither
geniculate nor nodulose, unbranched or branched, branching
often at an angle of 45−90°, just below the apex or at a lower level,
branches sometimes only as short denticle-like prolongations just
below a septum, occasionally long, conidiophores 30−310(−460)
× 2.5−4 μm (on OA up to 900 μm long), septate, sometimes
distinctly constricted at septa, pale to medium brown or
www.studiesinmycology.org
olivaceous-brown, smooth, sometimes slightly rough-walled
at the base, walls somewhat thickened, sometimes slightly
attenuated towards the apex and distinctly swollen at the base,
with age conidiophores becoming darker and more thick-walled;
micronematous to semimacronematous conidiophores narrower,
paler, iliform to narrowly cylindrical-oblong, non-nodulose or only
slightly swollen at the apex, unbranched, 17−85 × (1−)2−2.5 μm,
with few septa or reduced to conidiogenous cells, pale brown or
subhyaline, smooth, walls unthickened or almost so, with a single
or up to seven subdenticulate, pronounced loci crowded at the
apex. Conidiogenous cells integrated, terminal and intercalary,
cylindrical-oblong, sometimes short geniculate at the apex,
often nodulose, swellings up to 5 μm wide, cells (4−)10−44 μm
long, loci often situated on swellings but not restricted to them,
mostly only a single swelling per cell, in terminal cells apex
usually head-like uni- or multilaterally swollen with up to eight
pronounced, subdenticulate to denticulate loci crowded at the
tip, in intercalary conidiogenous cells loci often sitting at about
the same level (arranged like a garland round about the stalk)
or situated on small lateral shoulders, loci 1−1.5(−2) μm diam,
thickened and darkened-refractive. Ramoconidia occasionally
formed, subcylindrical or cylindrical-oblong, 22−41 × 3−4(−5)
μm, 0(−1)-septate, base broadly truncate, 2−3.5 μm wide.
Conidia catenate, in densely branched chains, 1−4(−6) conidia
in the terminal unbranched part of the chain, branching in all
271
BenSch et al.
directions, straight, small terminal conidia subglobose, obovoid,
limoniform, sometimes globose, (2−)2.5−5(−6) × (1.5−)2−3 μm
(av. ± SD: 3.7 ± 1.0 × 2.2 ± 0.4), aseptate, apex broadly rounded,
intercalary conidia ovoid, ellipsoid or subcylindrical, 4−12(−17) ×
(1−)2−3(−4.5) μm (av. ± SD: 8.1 ± 2.7 × 2.8 ± 0.6), aseptate,
occasionally 1-septate, with up to 5(−7) distal hila, sometimes
cell lumen distinct, secondary ramoconidia ellipsoid, fusiform
to subcylindrical or cylindrical, (6−)7−25(−31) × (2−)2.5−4(−5)
μm (av. ± SD: 15.0 ± 5.8 × 3.2 ± 0.5), with (1−)2−6(−7) distal
hila, sometimes with 1−2 hila at the basal end, 0−1(−2)-septate,
sometimes distinctly constricted at septa, with age more
frequently septate, pale brown or pale olivaceous-brown, smooth,
occasionally irregularly rough-walled, walls unthickened or
almost so, attenuated towards apex and base, hila conspicuous,
subdenticulate to denticulate, 0.5−1.8(−2) μm diam, thickened
and darkened-refractive; microcyclic conidiogenesis occasionally
occurring with conidia forming secondary conidiophores.
Culture characteristics: Colonies on PDA attaining up to 84 mm
diam after 14 d, smoke-grey to grey-olivaceous or olivaceousgrey, reverse leaden-grey to olivaceous-black, woolly to luffy,
margin glabrous to feathery, grey-olivaceous to white, aerial
mycelium abundant, high, luffy, smoke-grey, dense, without
prominent exudates, sporulating. Colonies on MEA reaching 70−80
mm diam after 14 d, smoke-grey to pale olivaceous-grey, pale
olivaceous due to abundant sporulation, reverse olivaceous-grey,
woolly, luffy, margins narrow, glabrous to feathery, colourless to
white, sometimes radially furrowed and wrinkled, aerial mycelium
abundant, luffy, dense, high, pale olivaceous-grey, covering large
parts of the colony surface, growth low convex, few prominent
exudates formed, sporulating. Colonies on OA attaining 65−73
mm diam after 14 d, smoke-grey, pale olivaceous-grey to whitish
due to aerial mycelium, greenish grey towards margin, reverse
olivaceous-grey to iron-grey or leaden-grey, woolly-luffy to felty,
margin colourless to white, narrow, glabrous, aerial mycelium high,
abundantly formed, luffy to felty, whitish, growth lat to low convex,
mostly without prominent exudates, sporulating.
Substrate and distribution: On different host plants isolated from
dead leaves, twigs, stems, wood and other organic matter, also
isolated from air, bread, soil and water; cosmopolitan but especially
common in the tropics.
Additional specimens examined: Australia, Cairns, isol. from Callistemon viminalis
(Myrtaceae), 18 Aug. 2006, P.W. Crous (CPC 13222). Brazil, Fortaleza, isol. from
a rust fungus, 30 Jul. 2005; U. Braun (CPC 12223); Pernambuco, isolated from
Carica papaya (Caricaceae), 26 Nov. 1960, A.F. Vital 27 (IMI 83634). Burundi,
isol. from a fruit, J. Rammelo, isol. by B.P.R. Vittal, ident. W. Gams (CBS 117.79).
Indonesia, isol. from Musa sp. (Musaceae), 2004, M. Arzanlou (CPC 11612). Iran,
isol. from Citrus ×aurantium (Rutaceae), 2004, W. Gams (CPC 11555). Ivory Coast,
Abidjan, isol. from Musa sp., 10 Jan. 2005, Kone Daouda, CL1 ra (CBS 126501 =
CPC 14410). Mozambique, isol. from Musa sp., coll. A. Viljoen, isol. P.W. Crous
(CPC 10538, 10539). Nigeria, isol. from fruit (CBS 262.80). Polynesia, reserve
Pun Kukui, in forest, isol. from banana, 2006, coll. I. Budenhagen, isol. P.W. Crous
(CPC 12794, 12795). Russia, Novgorodskaya Oblast’, Okulovskij Rajon, village
Zarechnaya, on wood and bark of Populus tremula (Salicaceae), 22 Jul. 2006, D.A.
Chabounin (HAL). South Africa, Durban, Durban Botanical Garden near Reunion,
-29.85, 31.0167, isol. from Strelitzia sp. (Strelitziaceae), 2005, coll. W. Gams, isol.
P.W. Crous (CPC 11805). South Korea, Jeju, N33≡ 27’25’’ E126º33’40’’, isol. from
Pseudognaphalium afine (Asteraceae), 28 Oct. 2005, coll. H.-D. Shin, isol. P.W.
Crous, preserved as “Passalora sp.” (CPC 10882). USA, Delaware, Wilmington, on
dead leaves of Rosa sp. (Rosaceae), 11 Dec. 1883, A. Commons (PH 01020444);
Louisiana, Baton Rouge, isol. from Magnolia sp. (Magnoliaceae), 8 Sep. 2007,
P.W. Crous (CPC 14250); Utah, Cache Co., Cache national Forest, Logan Canyon,
isolated from Penstemon cyananthus (dried culture) (Plantaginaceae), 1 Sep. 1990,
C.T. Rogerson (NY); Weber Co., Ogden, isolated from dead twigs of Mahonia
272
aquifolium (dried culture) (Berberidaceae), 10 Apr. 1983, C.T. Rogerson (NY).
Yugoslavia, isolated from Adriatic sea water, 4 Sep. 1974, M. Cvetkovic U-69 (IMI
187598, as C. cladosporioides).
Cladosporium tenuissimum s. lat. / Lineage 1 (see Bensch et al. 2010, Fig. 1c):
Australia, Queensland, isol. from rock, Chillagoe Mungana Caves National Park, P.W.
Crous (CPC 13252). Bali, bat cave, isol. from soil, 2000, coll. J.C. Frisvad, isol. B.
Andersen (BA 1737 = CPC 14370). India, isol. from Dalbergia sp. (Fabaceae), 2004,
coll. W. Gams, isol. P.W. Crous (CPC 11130); Chandigarh, 30.7372, 76.7872, isol.
from Citrus sp. (Rutaceae), 3 Jan. 2004, W. Gams (CPC 11132). Laos, Vientiane
Capital, Xaythany District, Xay Villiage, isol. from leaves of Basella alba [= B. rubra]
(Basellaceae), 4 Jan. 2007, coll. P. Phengsintham, isol. P.W. Crous (CPC 14196); isol.
from Shorea siamensis (Dipterocarpaceae), 22 Jan. 2007, coll. P. Phengsintham, isol.
P.W. Crous (CPC 13732). Thailand, isol. from Acacia mangium (Fabaceae), coll. W.
Himaman, isol. P.W. Crous (CPC 11521, 11929). Venezuela, Cabruta, Mochimo Bay,
isol. from a decayed branch under water, 2007, coll. K. Lyhne, isol. B. Andersen (BA
1710 = CPC 14311); Rojo, Mochimo Bay, isol. from sediment, red mangrove, 2007,
coll. K. Lyhne, isol. B. Andersen (BA 1711 = CPC 14312).
Notes: Cladosporium tenuissimum is a common saprobic
hyphomycete comparable and confusable with C. cladosporioides,
but genetically as well as morphologically distinct as demonstrated
in Bensch et al. (2010) and discussed under “notes” of the latter
species. Material distributed in Roumeguère, Fungi Sel. Gall. Exs.
5295, issued in 1890, seems to be topotype material rather than
syntype material.
On SNA plates conidiophores of C. tenuissimum can become
darker and more thick-walled with age and conidia more frequently
septate. On OA and PDA the conidiophores are very long and darker,
medium to dark olivaceous-brown, surprisingly forming several
nodules quite distant from each other, as in the closely allied C.
oxysporum, which are never formed in vivo. However, C. oxysporum
does not form such characteristically nodose conidiophores on OA
and PDA, but does so on SNA and in vivo. Therefore, the formation
of such swellings in culture has to be cautiously taken into
consideration as diagnostic character. Otherwise, the data given in
the general descriptions of C. cladosporioides and C. tenuissimum
in Ellis (1976) and Ho et al. (1999) could be conirmed.
Cladosporium stanhopeae, a species described on Stanhopea
(Orchidaceae) from Germany (Schubert & Braun 2004, Schubert
2005b) resembles C. tenuissimum but is tentatively maintained as
a separate species until isolates from that host can be included in
molecular studies.
Various authors described and recorded C. tenuissimum
collections isolated from and classiied to be mycoparasitic on rust
fungi (e.g. Sharma & Heather 1981, 1988, Moricca et al. 1999).
Assante et al. (2004) used one strain of C. tenuissimum out of
the material studied by Moricca et al. (1999), carried out detailed
histological examinations by means of TEM, and found close
interactions between Cladosporium hyphae and uredospores,
indicating that the fungus examined was a true mycoparasite.
However, the material concerned was not available and could
not be proven. Therefore, it remains unclear if C. tenuissimum
may occur as mycoparasite of rusts or if the authors concerned
confused the examined material with C. uredinicola. Heuchert et
al. (2005) re-examined several collections of “C. tenuissimum” on
rusts, but all of them proved to be identical with C. uredinicola.
157. Cladosporium trillii Ellis & Everh., Proc. Acad. Nat.
Sci. Philadelphia 47: 430. 1895. Figs 317, 318.
Holotype: USA, Washington, Pullman, on leaves of Trillium
petiolatum (Melanthiaceae), Jun. 1894, C.V. Piper, no. 341, mixed
infection with Phyllosticta trillii Ellis & Everh. (NY).
the genuS Cladosporium
= Heterosporium trillii Ellis & Everh., Proc. Acad. Nat. Sci. Philadelphia 46:
382. 1894 [holotype: NY].
≡ Cladosporium trillii (Ellis & Everh.) J.C. David, Mycol. Pap. 172: 94.
1997. nom. illeg., non C. trillii Ellis & Everh., 1895.
≡ Cladosporium trilliicola J.C. David, Schlechtendalia 11: 88. 2004.
Lit.: David (1997: 94), Schubert (2005b: 144–146).
Ill.: David (1997: 89, ig. 22 C–F; 95, ig. 25), Schubert (2005b: 145,
ig. 70, pl. 32, igs A–D).
In vivo: Leaf spots amphigenous, subcircular-elliptical, 2–7(–20)
mm wide, with a whitish grey membranous centre, turning greyish
brown with dense fructiication, margin narrow, brown to reddish
brown, sometimes surrounded by a yellowish brown halo. Colonies
amphigenous, effuse, scattered, in small tufts, loose to somewhat
dense, caespitose, brown. Mycelium internal, subcuticular to
intraepidermal; hyphae branched, 2–7 μm wide, septate, sometimes
slightly constricted at the septa, occasionally with small swellings, up
to 10 μm wide, subhyaline to pale olivaceous or olivaceous-brown,
smooth, walls slightly thickened. Stromata small, compact, 15–40 μm
diam, composed of subglobose to somewhat angular cells, 5–10 μm
wide, pale to medium olivaceous-brown, smooth, walls thickened.
Conidiophores in small loose fascicles, arising from stromata,
erumpent through the cuticle or emerging through stomata, erect,
straight to somewhat lexuous, often geniculate-sinuous, subnodulose
or slightly nodulose, unbranched or rarely once branched, 30–155
× 6–9(–10) μm, septate, with only few septa, septa sometimes not
very conspicuous, pale brown to medium reddish brown, smooth,
Fig. 317. Cladosporium trillii (NY). Symptoms, conidiophores and conidia in vivo.
Scale bar = 10 µm. K. Bensch del.
Fig. 318. Cladosporium trillii (NY). A. Symptoms. B. Conidiophores and conidia. C. Conidium. D. Geniculate, somewhat nodulose conidiophore and conidia. Scale bars = 10
(B–D) µm.
www.studiesinmycology.org
273
BenSch et al.
walls thickened but one-layered, up to 0.5 μm wide, often somewhat
swollen at the base, up to 11 μm wide, slightly attenuated towards
the apex, occasionally enteroblastically proliferating. Conidiogenous
cells integrated, terminal and intercalary, 6–45 μm long, proliferation
sympodial, often geniculate-sinuous, subnodulose or slightly
nodulose, conidiogenous loci situated on small unilateral shoulders
or multilateral swellings, protuberant, short cylindrical, 1.5–2(–2.5)
μm diam, mostly 1 μm high, clearly differentiated in a somewhat
raised central dome and a periclinal rim, thickened, somewhat
darkened-refractive. Conidia catenate, in unbranched chains, more
or less straight, obovoid, broadly ellipsoid to mostly cylindrical, (5–)
11–36(–41) × (3.5–)6–11(–12) μm, (0–)1–4(–5)-septate, sometimes
slightly constricted at the septa, pale olivaceous to olivaceous-brown,
distinctly verruculose or verrucose, walls only slightly thickened, apex
and base rounded or slightly attenuated, hila protuberant, 1–2(–2.5)
μm diam, thickened, darkened-refractive; occasionally microcyclic
conidiogenesis occurring.
Substrate and distribution: On Trillium spp.; North America – Trillium
ovatum (USA, ID, MT, WA), T. petiolatum (USA, WA), Trillium sp.
(USA, WA).
Additional specimen examined: USA, Idaho, Latah Co., on Trillium ovatum, 14 Jul.
1893, C.V. Piper 128 (NY, holotype of Heterosporium trillii).
Notes: In 1894, Ellis & Everhardt described the new species
Heterosporium trillii as causing leaf spots on Trillium ovatum,
eventually killing off the leaves. One year later they introduced
Phyllosticta trillii on Trillium petiolatum and mentioned a
Cladosporium trillii occurring on the same spots for which they gave
a short description (Ellis & Everhardt 1895). David (1997), who
revised the fungi previously referred to Heterosporium, examined
the type material of Heterosporium trillii and assigned this species to
Cladosporium, but failed to recognise that the name Cladosporium
trillii already existed. Later, C. trilliicola was published as new name
for this homonym (Dugan et al. 2004). However, a re-examination
of type material of Cladosporium trillii revealed that both species
are conspeciic.
Cladosporium ossifragi and C. variabile are morphologically
comparable with C. trillii but C. ossifragi differs in the somewhat
narrower, longer conidiophores, generally shorter and somewhat
narrower conidia and the degree of septation; and C. variabile, the
causal agent of leafs spot of spinach, possesses tortuose, spirally
twisted aerial hyphae, wider and somewhat longer conidia, (6.5–)
10–45(–55) × (5–)7–14(–17) μm, and wider conidiogenous loci and
hila, (1–)2–3(–3.5) μm diam.
158. Cladosporium uredinicola Speg., Anales Mus. Nac.
Hist. Nat. Buenos Aires 23: 122–123. 1912. Figs 319–322.
Holotype: Argentina, Salta, near Calilegua, on sori of Puccinia
cestri (Pucciniaceae) on Cestrum pubescens (Solanaceae), Nov.
1911, C. Spegazzini (LPS 13073). Permanent slides: AUA, IMI
87162a.
Lit.: Saccardo (1931: 798), Sutton (1973: 40), Ellis (1976: 330),
Traquair et al. (1984), Ellis & Ellis (1985: 571, 1988), Morgan-Jones
& McKemy (1990), Sheta (1996), Ho et al. (1999: 142), Zhang et
al. (2003: 168–170), Heuchert et al. (2005: 41–46), Dugan & Glawe
(2006), Bensch et al. (2010: 81–82).
Ill.: Sutton (1973: 41, ig. 19A), Ellis (1976: 331, ig. 249), Morgan274
Fig. 319. Cladosporium uredinicola (IMI 254519). Conidiophores arising from
hyphae and conidia in vivo. Scale bar = 10 µm. B. Heuchert del.
Jones & McKemy (1990: 189, pl. 1; 191, ig. 1; 193, ig. 2; 195, ig.
3; 197, pl. 2; 199, pl. 3), Ho et al. (1999: 143, ig. 49), Zhang et al.
(2003: 169, ig. 117), Heuchert et al. (2005: 42–44, igs 16–18).
Exs.: Braun, Fungi Sel. Exs. 53.
In vivo: Colonies on rust sori, pale olivaceous or olivaceous-grey,
loose to dense, caespitose, loccose, effuse. Mycelium supericial,
occasionally immersed; hyphae branched, 2–5(–7) μm wide,
septate, often constricted at the septa, some cells swollen, up to 8
μm diam, sometimes aggregated, forming dense hyphal nets, pale
olivaceous-brown, occasionally subhyaline, smooth or sometimes
faintly rough-walled, walls unthickened to slightly thickened.
Stromata lacking. Conidiophores solitary, arising from hyphae,
lateral and terminal, or aggregated in loose groups, erect, rarely
decumbent, straight to curved, often somewhat geniculate-sinuous,
unbranched or occasionally branched (at an acute angle of about
30°), (5–)20–230(–300) × 2–5.5 μm, narrowed towards the apex,
0–8-septate, without any constrictions, pale to medium olivaceousbrown, paler towards the apex, sometimes even subhyaline at the
tips, smooth to faintly verruculose, walls slightly thickened, 0.5–0.75
μm wide, tips unthickened. Conidiogenous cells integrated, terminal
and intercalary, cylindrical or occasionally subclavate, 9–59(–75) μm
long, polyblastic, proliferation sympodial, with up to 11 conidiogenous
loci per cell, sometimes aggregated, slightly protuberant, often on
small shoulders or swellings caused by sympodial proliferation, 1–2
μm diam. Ramoconidia rare. Conidia usually in branched chains,
straight, rarely somewhat curved, subglobose, obovoid, limoniform,
narrowly ellipsoid, fusiform, subcylindrical, subclavate, subhyaline,
pale olivaceous to olivaceous-brown, smooth or almost so, walls
uniformly thin or only very slightly thickened; small terminal conidia
and intercalary conidia 3–18 × 2–5 μm, 0–1-septate; secondary
the genuS Cladosporium
greenish black. Hyphae septate, olivaceous-brown, smooth to
verruculose, (2.5–)3.3–4(–5.6) μm wide. Conidiophores up to
290 μm long, but usually 120–170 μm, (2.5–)3.3–3.7(–4.1) μm
wide, simple or occasionally branched, pluriseptate, smooth to
verruculose, light to dark olivaceous. Ramoconidia and secondary
ramoconidia oblong-cylindrical, (6–)9.9–12.2(–15.6) × (2.5–)3.3–
3.6(–4.1) μm, 0–1-septate, pale olive. Conidia (small terminal
and intercalary conidia) (5–)5.9–8.2(–16.5) × (2.9–)3.3–3.6(–3.8)
μm. Colonies on PDA (Morgan-Jones & McKemy 2000) at 25 °C
attaining 43–48 mm diam in two weeks, olive to dark green, evenly
lanose or with outer perimeter (18–22 mm wide), smooth, velvety.
Hyphae variable, of two main types, either thin-walled, 2–4 μm
wide, not constricted at the widely spaced septa, or hyphae with
somewhat thicker walls, up to 7 μm wide, constricted at the septa
which occur at frequent intervals, with chlamydospore-like cells,
5–7.5 μm diam. Conidiophores up to 600 μm long and 3–5 μm wide,
often with unilateral branches, up to 150 μm long. Ramoconidia
subhyaline to pale olivaceous, up to 60 μm long, mostly 15–25 μm
long, and 4–5 μm wide, up to 5-septate.
Fig. 320. Cladosporium uredinicola (IMI 171548, at the lower limit of the variability
of the species). Conidiophores arising from hyphae, microcyclic conidiogenesis and
conidia in vivo. Scale bar = 10 µm. B. Heuchert del.
Fig. 321. Cladosporium uredinicola (IMI 183694 and IMI 183695). Conidiophores
arising from hyphae or aggregated in loose groups, microcyclic conidiogenesis and
conidia in vivo. Scale bar = 10 µm. B. Heuchert del.
ramoconidia 8–39 × 3–6.5(–8) μm, 0–3(–5)-septate, without any
constrictions, occasionally subhyaline, apex and base rounded,
occasionally somewhat wider at the apex, with up to seven hila; hila
slightly protuberant, rarely denticle-like, darkened-refractive, 0.5–1.5
μm diam, basal hila of larger conidia usually somewhat wider, 2–3 μm
diam; occasionally with microcyclic conidiogenesis.
In vitro: Colonies on MEA (Ho et al. 1999) attaining 50 mm diam
at 25 °C in 2 w, greyish olivaceous to olivaceous, velvety, reverse
www.studiesinmycology.org
Substrate and distribution: Hyperparasitic on telia and uredia
of rusts (Pucciniales), especially Cronartium and Puccinia,
also on downy mildews (Peronosporales) and powdery mildew
fungi (Erysiphales); Asia (Hong Kong, India, Iran, South Korea),
Australasia (Australia, New Zealand), Caribbean, Europe (Czech
Republic, Germany, Netherlands, UK), North America (Canada,
USA) and South America (Argentina, Brazil) – Chrysocyclus cestri
(South America), Cronartium fusiforme f. sp. quercum (USA, AL),
Cronartium ribicola (UK), Erysiphe euonymicola on Euonymus
japonicus (Iran), E. kusanoi on Celtis (Korea), Gymnosporangium
nelsonii on Amelanchier lorida (Canada, BC), G. sabinae on
Pyrus communis (Germany), Melampsora coleosporides on Salix
babylonica (Australia, NSW), M. laricis-populina on Populus
deltoides (Australia, NSW), M. laricis-populina on Populus sp. (New
Zealand), M. laricis-populina on Populus gelrica (Australia, NSW),
Peronospora arborescens on Papaver somniferum (India, UP), P.
gaeumannii on Argemone mexicana (India, UP), Phragmidium sp. on
Rubus allegheniensis (USA, NY), Phyllactinia angulata on Quercus
(USA, NY), Phyllactinia guttata on Corylus avellana (USA, WA),
Pleochaeta shiraiana on Celtis (Korea), Puccinia allii on Allium sp.
(UK), P. artemisiicola on Artemisia campestris (Germany), P. cestri
on Cestrum pubescens (Argentina), P. coprosmae on Coprosma
macrocarpa (New Zealand); P. horiana on Chrysanthemum
ssp. (Brazil; USA, FL), P. jaceae on Grossheimia macrocephala
(≡ Centaurea macrocephala) (Germany), P. malvacearum on Alcea
rosea (Germany), P. melanocephala on Saccharum oficinarum
(Australia), P. melanocephala on Saccharum sp. (India), P. recondita
on Triticum sp. (UK), P. sessilis on Arum maculatum (UK), P. vincae
on Vinca sp. (Czech Republic), P. violae on Viola odorata (Canada),
Puccinia sp. on Carex acutiformis (the Netherlands), Tranzschelia
pruni-spinosae (Hong Kong), Triphragmium ulmariae (UK).
Records without particular rust fungi: Pulsatilla dahurica
(China: Heilongjiang, Jilin). Records without any hosts: Cuba, New
Zealand.
Additional specimens examined: Australia, Brisbane, BSES Eight Mile Plains
Sugar Exp. Stn., on Puccinia melanocephala on Saccharum oficinarum, 5 Nov.
1980, O.W. Sturgess (IMI 254519, permanent slide); NSW, 10 km N of Kyogle, on
Melampsora coleosporides on Salix babylonica, 26 Feb. 1979, C.A. Nicholson (DAR
33593); Snowy Mountains Authority Plantation, Tumur, on M. laricis-populina on
Populus deltoides, 4 Mar. 1974, R.C. Nielsen (DAR 24066); on M. laricis-populina
on Populus gelrica, 5 Mar. 1974, R.C. Nielsen (DAR 24060). Canada, BC, Sarno,
on Gymnosporangium nelsonii on Amelanchier lorida, 11 Jul 1935, G.G. Hedgcock
275
BenSch et al.
Fig. 322. Cladosporium uredinicola (IMI 254519). A–B. Conidiophores and conidia in vivo. C–D. Details of the coronate scar structure on conidia. Scale bars = 5 (C), 10 (B, D),
20 (A) µm.
(BPI 427380, as C. peridermiicola); W of Nellway, 24 Jul 1936, G.G. Hedgcock (BPI
427381, as C. peridermiicola). Germany, Brandenburg, Kr. Prignitz, Triglitz, on
Puccinia malvacearum on Alcea rosea, 3 Oct. 1898, O. Jaap (HBG, as C. aecidiicola);
Hessen, Frankfurt/Main, botanical garden, on P. malvacearum on Alcea rosea, 7 Oct.
2004, R. Kirschner 2221 (HAL); Schöneck near Frankfurt/Main, on Gymnosporangium
sabinae on Pyrus communis, 11 Jul 2004, J. Gossmann (herb. R. Kirschner); SachsenAnhalt, Halle, botanical garden, on Puccinia jaceae on Grossheimia macrocephala,
16 Jul. 2004, U. Braun (HAL); on P. malvacearum on Alcea rosea, 22 Apr. 2004, B.
Heuchert (HAL) and Braun, Fungi Sel. Exs. 53 (HAL); Kr. Wittenberg, Kemberg, on
Puccinia artemisiicola on Artemisia campestris, 30 Aug. 2002, H. Jage (herb. Jage, Nr.
2690/02). India, Udaipur University, on Puccinia melanocephala on Saccharum sp.,
K.L. Kothari (IMI 245023); UP, Ghazipur, on Peronospora arborescens on Papaver
somniferum, 10 Feb. 1974, S.L. Singh (IMI 183695); on P. gaeumannii on Argemone
mexicana, 10 Feb. 1974, S.L. Singh (IMI 183694). Iran, Guilan, Rasht, on Erysiphe
euonymicola on Euonymus japonicus, 25 Jul. 2004, S.A. Khodaparast (HAL). New
Zealand, Auckland, Wattle Bay, on Puccinia coprosmae on Coprosma macrocarpa,
5 Jul. 1982, P.A. Maddison (PDD 43020, as C. cladosporioides). South Korea, Juju,
Halla Arboretum, on Erysiphe kusanoi and Pleochaeta shiraiana, 11 Dec. 2006, H.D.
Shin (KUS-F22520, HAL 2092 F). UK, on Puccinia recondita on Triticum sp., Dec.
1972, D.J. Allen (IMI 171548); Scotland, Dungairney Gdns., Bridge of Earn, Perthshire,
on Cronartium ribicola, 1979, A.P. Bennell (IMI 252337). USA, Alabama, Lee County,
Auburn, hyperparasitic on Cronartium fusiforme (Cronartiaceae, Uredinales) on
leaves of Quercus nigra (Fagaceae), 20 May 1982, W.D. Kelley (ATCC 46649 =
CPC 5390); New York, Bronx County, southwest of azalea plantings, The New York
Botanical Garden, on Phragmidium sp. on Rubus allegheniensis, 28 Nov. 1979, C.T.
Rogerson (NY, as C. tenuissimum); west of North Bridge, The New York Botanical
Garden, on Phyllactinia angulata on Quercus sp., 6 Oct. 1986, C.T. Rogerson (NY,
as C. tenuissimum).
Notes: A single strain of C. uredinicola (CPC 5390 = ATCC
46649) has been included in molecular studies within the C.
cladosporioides complex by Bensch et al. (2010), but did not
sporulate. Morgan-Jones & McKemy (1990), who examined this
strain previously, conirmed its identity based on morphology, but
mentioned that the conidiophores were somewhat longer and the
conidia somewhat wider and more frequently septate as in other
collections. Phylogenetically, the strain concerned is allied to C.
funiculosum and C. pseudocladosporioides (see Bensch et al.
2010, ig. 1, part b).
The type material of C. uredinicola is in poor condition
(Sutton 1973) and was, therefore, not re-examined. Sutton (l.c.)
examined the holotype and deposited a permanent slide at IMI.
Ho et al. (1999) und Morgan-Jones & McKemy (1990) examined
C. uredinicola in culture and published detailed descriptions of
its features in vitro. The latter authors showed that growths and
morphology are dependent on the particular substrates and that the
conidiophores are usually much shorter, less branched and darker
in nature. The conidia are usually shorter and have only few septa.
In literature, various collections with slightly deviating
characteristics have been discussed, e.g., two samples from
Tranzschelia pruni-spinosae which were only tentatively assigned
to C. uredinicola by Sutton (1973) since most conidiophores were
shorter than 100 μm and narrower than 3.5 μm, whereas MorganJones & McKemy (1990) considered these collections to be
276
conspeciic with C. uredinicola, based on the morphology of the
conidiophores and conidia. Our own examinations have shown
that C. uredinicola is fairly variable, i.e., some specimens are very
delicate (colonies barely visible under stereomicroscopy), and then
the conidiophores and conidia are often at the lower limit of the
variability (see Heuchert et al. 2005, ig. 17), in which the collection
on Puccinia recondita on Triticum sp. displays conidiophores
simple in structure and conidia with a restricted range of size and
septation. These results support the inclusion of the samples from
Tranzschelia pruni-spinosae in C. uredinicola.
Two collections on downy mildews (Peronospora spp., deposited
at IMI, see Heuchert et al. 2005, ig. 18) have been studied and proved
to be morphologically indistinguishable from C. uredinicola, which is
in agreement with Morgan-Jones & McKemy (1990), who examined
these samples as well. Records of C. uredinicola on powdery mildew
fungi, viz., Erysiphe euonymicola on Euonymus japonicus (HAL)
and Phyllactinia angulata on Quercus sp. (NY), are new and also
surprising, but morphologically the collections concerned are not
separable from collection on rust fungi. However, the true afinity of
such collections has to be proven on the base of molecular sequence
analyses. Some other isolates from powdery mildew fungi have been
made and included in morphological and molecular studies such as C.
exile and C. phyllactiniicola, but proved to represent phylogenetically
distinct species with obvious morphological differences (see Bensch
et al. 2010, discussion).
Strain CBS 306.84, isolated from urediniospores of Puccinia
allii in the UK, and identiied as C. uredinicola proved to be not
conspeciic with CPC 5390. Morphologically it belongs to the C.
cladosporioides s. lat. complex but phylogenetically it is different
from C. cladosporioides s. str. clustering apart from this clade (see
Bensch et al. 2010, ig. 1, part b vs. c). Representative strains close
to the type material with regard to host and geographical origin are
not yet available. Therefore, an epitypiication of C. uredinicola has
not yet been proposed.
159. Cladosporium ushuwaiense Speg., Bol. Acad. Nac.
Ci. 27(4): 399. 1924, as “ushuwaiensis”. Figs 323, 324.
Holotype: Argentina, Tierra del Fuego, Ushuwaia, on dead leaves
of Berberis ilicifolia (Berberidaceae), 18 Jan. 1924, C. Spegazzini
(LPS 13.144).
Lit.: Saccardo (1972: 1340), Farr (1973), Schubert (2005b: 146–
148).
Ill.: Schubert (2005b: 147, ig. 71, pl. 32, igs E–H).
In vivo: On the lower leaf surface as greyish or greyish brown
discolorations, covering large areas or even the entire leaf surface.
the genuS Cladosporium
Fig. 324. Cladosporium ushuwaiense (LPS 13.144). A. Symptoms. B. Conidiophores
and conidia. C. Overview, fascicle of conidiophores. D. Conidiophore with percurrent,
enteroblastic proliferation and distinctly thickened walls. Scale bars = 10 (B, D), 50
(C) µm.
refractive. Conidia catenate, in branched chains, subglo-bose,
obovoid, narrowly to broadly ellipsoid or subcylindrical, 3–15(–20) ×
3–7(–9) μm, 0–1(–2)-septate, sometimes slightly constricted at the
septa, pale to medium brown or olivaceous-brown, almost smooth
to verruculose, slightly to distinctly thick-walled, often two-layered,
protoplasm often aggregated at the septa and walls appearing to be
thickened and with a more or less large and paler cavity in the centre
of the cells, apex and base more or less rounded or attenuated, with
a single to several apical hila, protuberant, slightly convex to short
cylindrical, 0.5–1.5(–2) μm diam, thickened, somewhat darkenedrefractive; microcyclic conidiogenesis not observed.
Fig. 323. Cladosporium ushuwaiense (LPS 13.144). Conidiophores and conidia in
vivo. Scale bar = 10 µm. K. Bensch del.
Colonies hypophyllous, effuse, loosely scattered or somewhat
denser, in tufts, dark brown, villose to bristle-like. Mycelium internal;
hyphae branched, 2–4 μm wide, septate, sometimes slightly
constricted at the septa, pale brown or pale olivaceous-brown,
smooth, walls somewhat thickened. Stromata substomatal to
intraepidermal, small to extended, several layers deep, compact,
composed of more or less angular to irregularly polygonal cells,
5–13 μm wide, pale to medium brown or medium dark brown,
smooth, thick-walled. Conidiophores solitary or mostly in small
fascicles, 2–10 per fascicle, arising from stromata, emerging
through stomata or erumpent through the cuticle, erect, straight to
slightly lexuous, especially near the apex, cylindrical-oblong, mostly
distinctly attenuated towards the apex, somewhat geniculate and
subnodulose towards the apex (in connexion with conidiogenesis),
unbranched or apically branched, once to several times, 80–310
μm long or even longer, 5–15 μm wide near the base, 3–5(–6)
μm at the apex, pluriseptate, medium to mostly dark brown, paler
towards the apex, almost smooth to asperulate or verruculose
throughout, walls thickened, usually distinctly two-layered, 0.5–2(–
3) μm wide, occasionally somewhat swollen at the base, sometimes
enteroblastically proliferating. Conidiogenous cells integrated,
terminal and intercalary, 6–36 μm long, proliferation sympodial,
subnodulose and somewhat geniculate in connexion with
conidiogenesis, with several conidiogenous loci often situated on
small shoulders or swellings, often somewhat crowded, protuberant,
subdenticulate, 1–1.5(–2) μm diam, thickened, somewhat darkenedwww.studiesinmycology.org
Substrate and distribution: On Berberis ilicifolia; Argentina.
Notes: This species is morphologically comparable with C.
apicale, known from Sri Lanka on Cycas circinalis, but the latter
species is quite distinct in having 0–3-septate, usually smooth and
somewhat narrower conidia with one-layered walls, somewhat
wider conidiogenous loci and hila and more frequently branched
conidiophores with somewhat thicker walls, 0.75–3 μm wide.
160. Cladosporium variabile (Cooke) G.A. de Vries, Contr.
Knowl. Genus Cladosporium: 85. 1952. Figs 325–328.
Basionym: Heterosporium variabile Cooke, Grevillea 5: 123. 1877.
≡ Helminthosporium variabile Cooke, Fungi Brit. Exs. Ser. 1, No. 360.
1870. nom. inval.
Holotype: UK, Wales, Montgomeryshire, Welshpool, Forden
Vicarage, on Spinacia oleracea (Chenopodiaceae), J.E. Vize,
Cooke, Fungi Brit. Exs. Ser. I, No. 360 (K). Epitype (designated by
Schubert et al. 2007b): USA, Washington, isolated from Spinacia
oleracea, 1 Jan. 2003, L. Du Toit (CBS H-19867). Ex-epitype
culture: CBS 121635 = CPC 12751, 12753.
= Cladosporium subnodosum Cooke, Grevillea 17(83): 67. 1889. [lectotype:
Ravenel, Fungi Amer. Exs. 294, NY].
= Davidiella variabilis Crous, K. Schub. & U. Braun, Stud. Mycol. 58: 152.
2007, as “variabile”.
Lit.: Ellis (1971: 315), Subramanian (1971: 295–296), Ellis & Ellis
(1985: 429), David (1995c, 1997: 94), IMI Descriptions of Fungi and
Bacteria 123, Sheet 1229 (1995), IMI Distribution Maps of Plant
277
BenSch et al.
Exs.: Cooke, Fungi Brit. Exs. Ser. I, No. 360; Poelt & Scheuer,
Reliqu. Petrak. 2359; Ravenel, Fungi Amer. Exs. 294; Săvulescu,
Herb. Mycol. Roman. 647; Winter, Fungi Eur. Extraeur. Exs. 3283;
Zahlbruckner, Krypt. Exs. 1498.
Fig. 325. Cladosporium variabile (NY, lectotype of C. subnodosum). Conidiophores
and conidia in vivo. Scale bar = 10 µm. K. Bensch del.
Fig. 326. Cladosporium variabile (CPC 12751). Macro- and micronematous
conidiophores and conidia in vitro. Scale bar = 10 µm. K. Bensch del.
Diseases 1229 (1996), Ho et al. (1999: 144), Zhang et al. (2003:
170–172), Schubert (2005b: 160–161), Schubert et al. (2007b:
150–153).
Ill.: Minoura (1966: 141, ig. 5D), Ellis (1971: 314, ig. 217 B), David
(1995c: 1, ig.; 1997: 97, ig. 26), Fuentes-Davila & Gabrielson
(1996: 54–55, igs 1–2), Ho et al. (1999: 145, ig. 50), Zhang et al.
(2003: 171, ig. 119), Schubert et al. (2007b: 150–152, igs 46–48).
278
In vivo: Leaf spots amphigenous, subcircular to oval, 1–4 μm
diam, pale olivaceous-brown to olivaceous-brown or dark brown
with dense fructiication, somewhat raised, surrounded by a raised
pale yellowish-ochraceous margin, later conluent. Colonies
amphigenous, effuse, densely caespitose, pale brown to brown,
velvety. Mycelium internal, subcuticular to intraepidermal; hyphae
branched, 2.5–11 μm wide, septate, often with swellings and
constrictions, sometimes appearing somewhat irregularly lobed,
subhyaline, pale olivaceous to pale medium olivaceous-brown
or somewhat reddish brown, smooth, walls somewhat thickened,
forming loose hyphal plates or aggregations. Stromata substomatal
or intraepidermal, composed of swollen hyphal cells, subglobose,
6–11 μm wide, walls somewhat thickened. Conidiophores fasciculate,
in loose small to moderately large fascicles, rarely also solitary,
arising from stromatic hyphal aggregations or swollen hyphal cells,
emerging through stomata or erumpent through the cuticle, erect
to subdecumbent, straight to often somewhat lexuous, cylindricaloblong, geniculate-sinuous, subnodulose to nodulose, with lateral
shoulders or multilateral swellings, unbranched, occasionally
once branched, 20–200 μm long or even longer, (3.5–)5–8.5 μm
wide, swellings 7–11 μm wide, septate, sometimes attenuated or
constricted at the septa, pale olivaceous-green to pale medium
olivaceous-brown or somewhat reddish-brown, paler towards
the apex, sometimes subhyaline, smooth to slightly roughened,
asperulate, walls more or less thickened, sometimes distinctly twolayered, up to 1 μm thick, apex often head-like swollen, base up to 11
μm wide. Conidiogenous cells integrated, terminal and intercalary,
20–68(–110) μm long, geniculate, subnodulose to nodulose,
proliferation sympodial, with a single or several loci conined to
unilateral shoulders or multilateral swellings, protuberant, short
cylindrical, coronate, distinctly differentiated in dome and rim but
dome not higher than the surrounding rim, (1–)2–3(–3.5) μm wide,
about 1 μm high, thickened, somewhat darkened-refractive. Conidia
in unbranched or branched chains, straight to curved, obovoid,
broadly ovoid, ellipsoid, subcylindrical or cylindrical, sometimes
slightly irregular or soleiform, (6.5–)10–45(–55) × (4.5–)6–14(–17)
μm, 0–4(–5)-septate, sometimes constricted at one of the median
septa, septa often appearing somewhat darkened, sometimes with
a single longitudinal septum, pale to medium olivaceous-brown
or somewhat reddish brown, verruculose to coarsely verrucose,
occasionally almost smooth with only few warts, walls thickened,
up to 2 μm thick, ends rounded or slightly attenuated, often with a
bulbous base, hila protuberant, short cylindrical, (1–)2–3(–3.5) μm
wide, about 1 μm high, thickened, somewhat darkened-refractive;
microcyclic conidiogenesis often occurring.
In vitro: Ascomata pseudothecial, black, supericial, situated on
a small stroma, globose, up to 250 μm diam, with 1–3 ostiolate
necks; ostioles periphysate, with apical periphysoids present;
wall consisting of 3–6 layers of dark brown textura angularis,
textura epidermoidea in surface view. Asci fasciculate, bitunicate,
subsessile, obovoid to broadly ellipsoid, straight to slightly curved,
8-spored, 70–95 × 18–28 μm; with pseudoparenchymatal cells
of the hamathecium persistent. Ascospores tri- to multiseriate,
overlapping, hyaline, with irregular lumina, thick-walled, straight
to slightly curved, fusoid-ellipsoidal with obtuse ends, widest near
the middle of the apical cell, medianly 1-septate, not to slightly
the genuS Cladosporium
Fig. 327. Cladosporium variabile (CPC 12751), anamorphic and teleomorphic state. A–C. Macronematous conidiophores. D, F. Micronematous conidiophores. E, G–H. Conidia.
I. Twisted aerial mycelium. J. Ascomata formed on nettle stem in culture. K. Surface view of ascomal wall of textura epidermoidea. L–M. Asci. N–P. Ascospores. Q. Ascus with
a sheath. Scale bars = 10 (A, D, G–J, K–N), 250 (J) µm.
www.studiesinmycology.org
279
BenSch et al.
Fig. 328. Cladosporium variabile (CPC 12753). A. Survey of hyphae that grow on the agar surface. Some of the fungal cells have a swollen appearance and could develop into
a “foot cell” that gives rise to a conidiophore. B. A number of aerial hyphae obstruct the swollen, large structures on the agar surface, which give rise to conidiophores. Some
of them appear ornamented. C. A series of conidia formed on a conidiophore (bottom of the micrograph). D. Detail of the ornamented conidia. The ornamentations are isolated
and dispersed. Note also the ornamentation-free scar zone and the hilum of the left cell. E. Two conidia behind an aerial hypha. F. Two conidiophores forming secondary
ramoconidia. Note the bulbous shape of the spore-forming apparatus. This micrograph is from an uncoated sample. Scale bars = 2 (D), 5 (E), 10 (A–C, F) µm.
constricted at the septum, at times developing a second septum
in each cell, several ascospores with persistent, irregular mucoid
sheath, (22–)26–30(–35) × (7–)7.5–8(–9) μm.
Mycelium immersed and supericial, irregularly branched, aerial
mycelium twisted and spirally coiled, 1–3 μm wide, septate,
sometimes with swellings or small lateral outgrowths, hyaline
to subhyaline, smooth, walls unthickened, hyphae which give
rise to conidiophores somewhat wider, 3–4.5 μm, subhyaline to
pale brown, almost smooth to minutely verruculose, sometimes
enveloped by a polysaccharide-like cover. Conidiophores usually
macronematous, but also micronematous, arising terminally
from ascending hyphae or laterally from plagiotropous hyphae.
Macronematous conidiophores erect, more or less straight to
lexuous, often distinctly geniculate-sinuous forming lateral
shoulders or unilateral swellings, sometimes zigzag-like or
somewhat coralloid, nodulose, swellings at irst terminal, then
becoming lateral due to sympodial proliferation, often as distinct
lateral shoulders, unbranched, sometimes once branched,
6–180 × (2.5–)3–6 μm, swellings (3–)6–11 μm wide, septate, not
constricted at the septa, pale to medium olivaceous-brown or
280
brown, usually verruculose, walls somewhat thickened, about 1 μm
thick, sometimes appearing to be two-layered. Conidiogenous cells
integrated, terminal and intercalary, cylindrical, nodulose to nodose,
with a single or two swellings per cell, swellings apart from each
other or formed in short succession, loci conined to swellings, up to
six per node, protuberant, 1–2 μm diam, thickened and darkenedrefractive. Micronematous conidiophores erect, straight to slightly
lexuous, unbranched, usually without swellings, iliform to narrowly
cylindrical, sometimes only as short lateral outgrowths of hyphae,
often almost indistinguishable from hyphae, up to 50 μm long,
1.5–2.5(–3) μm wide, longer ones pluriseptate, septa appear to
be somewhat more darkened, with very short cells, 4–12 μm long,
subhyaline to pale brown, smooth, walls unthickened or almost so.
Conidiogenous cells integrated, usually terminal, rarely intercalary,
cylindrical, non-nodulose, with a single, two or few conidiogenous
loci at the distal end, protuberant, up to 2 μm diam, thickened
and darkened-refractive. Conidia catenate, in branched chains,
straight, subglobose, obovoid, oval, broadly ellipsoid to cylindrical,
sometimes clavate, 4–26(–30) × (3.5–)5–9(–10) μm [av. ± SD, 16.8
(± 6.9) × 6.5 (± 1.4) μm], 0–3-septate, usually not constricted at
the septa, septa becoming sinuous with age, often appearing to
the genuS Cladosporium
be darkened, pale to medium or even dark brown or olivaceousbrown, verruculose to densely verrucose or echinulate (granulate
under SEM), walls slightly to distinctly thickened in larger conidia,
apex and base broadly rounded, sometimes broadly truncate or
somewhat attenuated, apex and base often appear to be darkened
or at least refractive, hila protuberant to somewhat sessile (within
the outer wall ornamentation), (0.8–)1–2 μm diam, thickened and
darkened-refractive; microcyclic conidiogenesis occurring.
Culture characteristics: Colonies on PDA attaining 29 mm diam
after 14 d at 25 ºC, olivaceous to olivaceous-grey or iron-grey, irongrey or olivaceous-grey reverse, velvety to powdery, margin regular,
entire edge to imbriate, almost colourless, aerial mycelium whitish
turning olivaceous-grey, sometimes reddish, greyish rose, woollyfelty, growth lat with elevated colony centre, somewhat folded or
radially furrowed, with age forming several very small but prominent
exudates, sporulation profuse. Colonies on MEA attaining 27 mm
diam after 14 d at 25 ºC, olivaceous-grey to iron-grey, white to pale
olivaceous-grey in the centre due to abundant aerial mycelium,
velvety, margin very narrow, colourless, more or less entire edge,
radially furrowed, aerial mycelium luffy to loccose, dense, growth
low convex with wrinkled and folded centre, without exudates,
sporulation profuse. Colonies on OA attaining 25 mm diam after
14 d at 25 ºC, iron-grey or olivaceous, margin regular, entire edge,
narrow, white, glabrous, aerial mycelium whitish, at irst mainly in
the colony centre, high, dense, loccose, growth lat, abundantly
sporulating, no exudates.
Substrate and distribution: Leaf-spotting fungus on Spinacia
oleracea; Africa (Morocco), Asia (China, India, Iran, Iraq, Pakistan,
South Korea, Turkey), Europe (Austria, Belgium, Bulgaria,
Cyprus, Czech Republic, Denmark, France, Germany, Greece,
Hungary, Italy, Montenegro, Netherlands, Norway, Romania,
Spain, Switzerland, Turkey, UK), North America (Canada, USA,
widespread).
161. Cladosporium varians U. Braun, Melnik & K. Schub.,
Mikol. Fitopatol. 42(3): 215. 2008. Figs 329–333.
Holotype: Russia, St. Petersburg, botanical garden of the V.L.
Komarov Botanical Institute, Russian Academy of Sciences, on
dead leaves of Catalpa bungei (Bignoniaceae), 15 Jan. 2007, V.A.
Mel’nik (LE 232350). Isotype: HAL 2061 F. Ex-type culture: CBS
126362 = CPC 13658, CPC 13659, 13660 [dried ex-type cultures:
HAL 2059 F (PDA), HAL 2060 F (SNA)].
= Cladosporium phyllogenum K. Schub., Mikol. Fitopatol. 42(3): 218. 2008.
(HAL 1845 F, holotype; CBS H-19870, isotype).
Lit.: Schubert (2005b: 117–120).
Ill.: Schubert (2005b: 118–119, igs 54–55, pl. 24, igs A–J), Braun
et al. (2008b: 216, ig. 1; 219–220, igs 2–3).
In vivo: Colonies on dead, still attached leaves, amphigenous, but
mainly hypophyllous, forming small to moderately large blackish
speckles or patches, fruiting often on and around leaf veins,
dense to effuse, dark greyish olivaceous to blackish or on living
leaves and petioles, hypophyllous, sometimes epiphyllous, at irst
occurring at the lower part of the leaves, especially near to or at leaf
veins, then spreading along leaf veins, subcircular, later conluent,
at irst without symptoms or lesions almost inconspicuous,
epiphyllous, irregular, paler green with small punctiform brown
areas, which later becoming larger, up to 1.5 mm wide, without
margin but surrounded by a pale greenish or yellowish green halo.
Colonies mostly hypophyllous, rarely epiphyllous, punctiform to
effuse, caespitose, loose to dense, pustule-like, medium to dark
olivaceous-brown or even blackish, velvety. Mycelium immersed
and external, supericial; hyphae branched, 2–7 μm wide, septate,
Additional specimens examined: Austria, garden near Stadlau, on leaves of
Spinacia oleracea, Jun., F. de Höhnel, Zahlbruckner, Krypt. Exs. 1498 (HBG).
Belgium, Bruxelles, botanical garden, Jun. 1884, E. Marschal, Winter, Fungi Eur.
Extraeur. Exs. 3283 (HBG, M, as Heterosporium variabile). Czech Republic,
Mährisch-Weißkirchen (Hranice), in a garden, Mar. 1935, F. Petrak, Poelt & Scheuer,
Rel. Petrak. 2359 (M-0057768, as H. variabile). Germany, Jun. 1949, Dr. v. Horn
(B 700006608, as C. macrocarpum); Baden-Württemberg, Karlsruhe, Durlach, Oct.
1948, Dr. J. Hruby (B 700006612, as C. macrocarpum); Bavaria, Freising, garden,
25 May 1898, Prof. Dr. Weiss (M). Italy, Siena, botanical garden, Apr. 1896, leg.?
(SIENA). Romania, Transilvania, distr. Alba-Aiud, 29 May 1932, T. Săvulescu & C.
Sandu, Săvulescu, Herb. Mycol. Roman. 647 (M, as “H. variabile”). Switzerland,
Graubünden, Fürstenach, Versuchsgarten, 1780 m, 22 Sep. 1901, A. Volkart (HBG,
as H. variabile). Turkey, Ankara, 29 Apr. 1942, H. Bremer, Petrak Pilzherbarium
(M). USA, Massachusetts, Amherst, May 1910, H.M. Jennison (M); South Carolina,
Aiken, Rav., Fungi Amer. Exs. 294 (NY: lectotype of C. subnodosum; Ravenel, Fungi
Amer. Exs. 294, e.g., BPI 427478, NY, PH: isolectotypes).
Notes: Cladosporium variabile is a biotrophic, leaf-spotting species,
morphologically belonging to the C. herbarum complex, in which it
is supericially similar to the saprobic C. macrocarpum, but differs
from the latter species in having distinctly larger and more frequently
septate conidia in vivo, often with distinctly darkened septa,
becoming sinuous with age and often with distinctly darkened apex
and base, as well as in forming twisted and spirally coiled aerial
mycelium in cultures, which have lower growth rates. In vivo the
conidia are usually longer, somewhat wider and more frequently
septate, (6.5–)10–45(–55) × (4.5–)6–14(–17) μm, 0–4(–5)-septate
(Schubert 2005b).
www.studiesinmycology.org
Fig. 329. Cladosporium varians (HAL 2061 F, isotype of C. varians). Mycelium,
conidiophores, conidiogenous cells, ramoconidia and conidia in vivo. Scale bar =
10 µm. U. Braun del.
281
BenSch et al.
Fig. 330. Cladosporium varians (HAL 1845 F, holotype of C. phyllogenum).
Conidiophores and conidiogenous cells in vivo. Scale bar = 10 µm. K. Bensch del.
occasionally with swellings, up to 9 μm diam, subhyaline, pale
olivaceous to medium olivaceous-brown, smooth or almost so, wall
thin, ≤ 1 μm. Stromata lacking, at most with diffuse aggregations of
swollen hyphal cells. Conidiophores solitary, arising from swollen
hyphal cells or supericial hyphae, lateral or terminal, erect to
decumbent, 15–500 × 2.5–9(–11) μm, straight to often lexuous,
cylindrical-iliform, subsetiform, barely geniculate to sometimes
several times mildly to distinctly geniculate-sinuous, rarely
subnodulose, intercalar swellings up to 11 μm wide, but swellings
without conidiogenous loci, simple, branched to multibranched,
often forming intricate aggregations of supericial hyphae and
conidiophores, differentiation between hyphae and branched
conidiophores often dificult or even impossible, pluriseptate
throughout, pale olivaceous to medium olivaceous-brown, smooth
or almost so, occasionally slightly rough-walled or rugose, thinwalled, ≤ 1 μm, or walls thickenend, often two-layered, lumen of
the cells sometimes appearing to be granular. Conidiogenous cells
integrated, usually terminal, cylindrical-oblong, 10–65 μm long,
slightly geniculate towards the apex or tips somewhat swollen,
unilaterally swollen or subdenticulate, with a single or several
conidiogenous loci, protuberant, obconically truncate, distinctly
coronate, 1.5–3 μm diam, thickened, somewhat darkened-refractive.
Ramoconidia subcylindrical to cylindrical-oblong, 17–60 × 4.5–8
μm, 0–3(–5)-septate, dingy olivaceous to olivaceous-brown, smooth
or almost so, walls thickened, sometimes even two-layered, base
broadly truncate, unthickened, 2–4.5 μm wide, somewhat refractive
or darkened, but not cladosporioid. Conidia polymorphous, very
variable in shape and size, catenate, in branched chains, straight
282
Fig. 331. Cladosporium varians (HAL 1845 F, holotype of C. phyllogenum).
Ramoconidia, secondary ramoconidia and conidia in vivo. Scale bar = 10 µm. K.
Bensch del.
to curved, small terminal conidia subglobose, ovoid, obovoid, 2–13
× 2–4.5 μm, aseptate, subhyaline, pale olivaceous, smooth or
almost so, hila 0.5–1 μm diam, intercalary conidia and secondary
ramoconidia (with a single or several hila at the apex) subglobose,
ovoid, obovoid, limoniform, ellipsoid, fusiform, subcylindrical or
cylindrical, 4–32 × 3–7 μm, 0–3(–4)-septate, not constricted at
septa, pale olivaceous to dingy olivaceous-brown, smooth or almost
so, walls more or less thickened, lumen of the cells sometimes
appearing to be granular, hila protuberant, short cylindrical, 0.5–2.5
μm diam, thickened, somewhat refractive to slightly darkened;
microcyclic conidiogenesis sporadically occurring.
In vitro: Mycelium mainly immersed, rarely supericial; hyphae
unbranched or branched, 1.5–7 μm wide, aerial hyphae narrower,
1.5–3 μm wide, septate, not constricted or slightly to distinctly
constricted at septa, pale olivaceous, olivaceous-brown or brown,
smooth or sometimes minutely verruculose or verruculose, thinwalled or almost so, sometimes hyphal cells distinctly swollen
at the base of conidiophores, up to 8 μm wide. Conidiophores
macronematous, sometimes also micronematous, terminally or
laterally arising from ascending or plagiotropous hyphae, solitary,
erect or ascending, straight to lexuous, cylindrical-oblong, nonnodulose, sometimes geniculate-sinuous towards the apex,
unbranched or branched, branches often formed as short, denticlelike lateral prolongations just below or at a septum, 25–300(–530)
× (2.5–)3.5–6(–7) μm, pluriseptate, upper septa just below potential
ramoconidia appear somewhat darker, refractive and thickened, pale
the genuS Cladosporium
slightly to distinctly constricted at septa, pale olivaceous to pale
olivaceous-brown, smooth or almost so, walls unthickened or
slightly thickened, slightly attenuated towards apex and base, hila
0.8–3 μm diam; microcyclic conidiogenesis not observed.
Culture characteristics: Colonies on PDA attaining 26–32 mm
diam after 14 d, dark green-olivaceous to dark grey-olivaceous,
olivaceous or iron-grey, sometimes slightly zonate, reverse greyolivaceous to olivaceous-grey or leaden-grey, velvety to powdery
or loccose, margin white, narrow or broad, regular, entire edge,
glabrous to somewhat feathery; aerial mycelium sparse, only at
few spots or diffuse, high, hairy to luffy, pale olivaceous-grey;
growth regular, lat to low convex with slightly elevated colony
centre, prominent exudates not formed, sporulation profuse,
two kinds of conidiophores formed, low and high ones. Colonies
on MEA reaching 17–22 mm diam after 14 d, olivaceous-grey,
grey-olivaceous to iron-grey surface and reverse, some colonies
glaucous-grey at margins, velvety to powdery, margins colourless
or white, regular, feathery, narrow to broad, aerial mycelium
sparse, diffuse, loosely loccose, growth low convex, radially
furrowed, sometimes wrinkled and folded in colony centre,
without prominent exudates, sporulation profuse. Colonies on
OA attaining 20–22 mm diam after 14 d, olivaceous-grey to irongrey, grey-olivaceous due to sporulation and pale olivaceousgrey due to aerial mycelium, reverse iron-grey to leaden-grey
or olivaceous-grey, surface somewhat zonate, loccose or luffy,
margin white, narrow, glabrous, aerial mycelium sparse, loosely
to densely loccose or luffy, growth lat or low convex, regular,
without prominent exudates, sporulating.
Fig. 332. Cladosporium varians (HAL 1845 F, holotype of C. phyllogenum).
Conidiophores and conidia in vivo. A. Geniculate conidiophore. B. Branched
conidiophore and conidia. C. Conidiophores with several conspicuous
conidiogenous loci. D. Conidia and external mycelium. E. Truncate, unthickened
base of a ramoconidium. F–G. Ramoconidia. H. Conidia showing coronate scar
structure. I–J. Conidia. Scale bars = 5 (E), 10 (C–D, F–J), 20 (A, B) µm.
olivaceous or olivaceous-brown, smooth to somewhat asperulate,
especially towards the base, walls somewhat thickened, 0.5 μm
wide; micronematous conidiophores narrower, paler and shorter,
lexuous, iliform, unbranched, 17–100 μm long or longer, 2–3(–3.5)
μm wide, septate, not constricted at septa, pale olivaceous or pale
brown, smooth or asperulate, walls unthickened. Conidiogenous
cells integrated, mostly terminal, but also intercalary, cylindricaloblong, non-nodulose, sometimes geniculate, 8–36(–96) μm long,
with a single or often up to three loci at the apex, often situated
on denticle-like prolongations, loci truncate or slightly convex,
(1.5–)2–3 μm diam, somewhat thickened and darkened-refractive.
Ramoconidia subcylindrical or cylindrical, 23–56(–64) × (3–)4–6(–
7) μm, 0–2(–3)-septate, concolorous with tips of conidiophores,
usually with up to three distal hila, not attenuated towards the
base or only slightly so, base broadly truncate, 3–4.5 μm wide,
unthickened and somewhat refractive. Conidia polymorphous,
numerous, catenate, in branched chains, branching in all directions,
up to ive conidia in the terminal unbranched part of the chain,
small terminal conidia globose, subglobose, ovoid to obovoid,
4–6(–8) × 2.5–3 μm (av. ± SD: 4.8 ± 1.0 × 2.8 ± 0.3), aseptate,
intercalary conidia ovoid to ellipsoid, 6–15(–18) × (2.5–)3–4(–4.5)
μm (av. ± SD: 9.9 ± 3.2 × 3.5 ± 0.4), 0–1-septate, with up to three
distal scars, secondary ramoconidia ellipsoid to subcylindrical or
cylindrical-oblong, (8–)11–33(–40) × (2.5–)3–6 μm (av. ± SD: 21.9
± 7.8 × 4.3 ± 0.7), 0–2(–5)-septate, mainly 1-septate, sometimes
www.studiesinmycology.org
Substrate and distribution: On plant material, sometimes also
endophytic; Asia (India), Europe (Germany, Russia, Slovenia).
Additional specimens examined: Germany, Sachsen-Anhalt, Halle (Saale),
Botanical Garden, on living leaves of Ulmus laevis (Ulmaceae), 9 Jul. 2004, K.
Schubert [HAL 1845 F, holotype of C. phyllogenum, isotype CBS H-19870 (dried
SNA plate); ex-type culture CBS 126360 = CPC 11327]. India, Habingirii, isol. from
leaf debris, 2004, W. Gams (CBS 126361 = CPC 11134). Slovenia, Gabrovka,
isol. from a fruit of Rosa canina (Rosaceae) attached to shrub, 3 Jan. 2008, H.-J.
Schroers (HJS 1038 = CPC 14975B).
Notes: Cladosporium varians belongs to the C. cladosporioides
complex, but differs from C. cladosporioides s. str. by its long,
frequently branched conidiophores, arising on the natural hosts
from supericial hyphae. Furthermore, the tips of the conidiogenous
cells are often somewhat swollen or unilaterally swollen, and the
ramoconidia have up to four septa, and subglobose conidia are
not abundant. Cladosporium tenuissimum is another comparable
species, which is, however, easily distinguishable by its setiform,
usually unbranched conidiophores, often with darker and thicker
walls, only 0–1-septate ramoconidia, and conidia ranging from
smooth to verruculose. Phylogenetically this species is closely allied
to C. paracladosporioides clustering as a sister to the latter species
(see Bensch et al. 2010, ig. 1, part a), but morphologically quite
distinct. Due to slighty morphological differences and distinct habits,
C. varians and C. phyllogenum have been described as two distinct
species. However, molecular sequence analyses clearly showed
that the two species are identical. The morphological differences
are within the range of intraspeciic variation.The ecology of
C. varians is not yeat clear. It has been collected as a saprobic
fungus on dead, but still attached leaves of Catalpa bungei, but
possibly lives also as an endophyte, only growing and sporulating
supericially under favourable external conditions with fructiication
283
BenSch et al.
Fig. 333. Cladosporium varians (CBS 126362). A–F. Macronematous conidiophores and conidial chains in vitro. G. Conidia. Scale bars = 10 µm.
mainly conined to and spread on veins, as demonstrated for the
type collection of C. phyllogenum.
162. Cladosporium velox Zalar, de Hoog & GundeCimerman, Stud. Mycol. 58: 181. 2007. Fig. 334.
Holotype: India, Charidij, isolated from Bambusa sp. (Poaceae), W.
Gams (CBS H-19735). Ex-type culture: CBS 119417.
Ill.: Zalar et al. (2007: 166, ig. 5 i, 180, ig. 14).
In vitro: Mycelium partly supericial partly submerged; hyphae
branched, 2–4 μm wide, septate, often with swellings and
constrictions, therefore appearing irregular in outline, pale brown
to pale olivaceous-brown, smooth, walls unthickened to slightly
thickened, often somewhat swollen at the base of conidiophores,
without extracellular polysaccharide-like material. Conidiophores
arising laterally or terminally from plagiotropous or ascending hyphae,
erect, straight to slightly lexuous, iliform to narrowly cylindricaloblong, sometimes slightly geniculate, due to this geniculation
slightly subnodulose, occasionally nodulose, (10–)25–150(–250) ×
(2–)2.5–4(–4.5) μm, unbranched or branched, branches often only
as short denticle-like prolongation below a septum, later branches
longer, dichotomously branched in an angle of 30–45°, 0–7-septate,
not constricted at septa, pale to medium olivaceous-brown, smooth,
walls somewhat thickened, often slightly attenuated towards the apex.
284
Conidiogenous cells integrated, mainly terminal but also intercalary,
sometimes conidiophores reduced to conidiogenous cells, iliform to
narrowly cylindrical-oblong, 20–42 μm long, proliferation sympodial,
with a single or several conidiogenous loci, often somewhat crowded
at the apex, subdenticulate, protuberant, 0.8–1.5 μm diam, thickened
and darkened-refractive. Ramoconidia rarely formed. Conidia
catenate, in branched chains, branching in all directions, terminal
chains with up to ive conidia, straight, small terminal conidia globose,
subglobose, ovoid, 2.5–4 × (1.5–)2–2.5 μm [av. (± SD) 3.3 (± 0.5) ×
2.2 (± 0.3)], aseptate, apex rounded, intercalary conidia limoniform
to narrowly ellipsoid, 3.5–10 × 2–3 μm [av. (± SD) 6.3 (± 2.0) × 2.4 (±
0.4)], aseptate, with up to 3(–4) distal hila, attenuated towards apex
and base, secondary ramoconidia narrowly ellipsoid to cylindricaloblong, straight to slightly curved, (7–)10–34(–42) × 2–3.5(–4.5) μm
[av. (± SD) 20.9 (± 9.1) × 2.7 (± 0.8)], 0–1-septate, not constricted
at septa, with up to 4(–5) distal hila, pale brown, smooth or almost
so to very inely verruculose, walls unthickened or almost so, slightly
attenuated towards apex and base, hila conspicuous, subdenticulate
to denticulate, 0.8–1.5 μm diam, thickened and darkened-refractive;
microcyclic conidiogenesis not observed.
Culture characteristics: Colonies on PDA reaching 35–45 mm diam,
grey-olivaceous to olivaceous, towards margins somewhat zonate,
becoming olivaceous-buff and dull green, reverse olivaceousblack, velvety to powdery, margin broad, white, regular, glabrous
to feathery, aerial mycelium absent or sparse, growth regular, low
convex, with numerous prominent exudates, sporulation profuse.
the genuS Cladosporium
Fig. 334. Cladosporium velox (CBS 119417) (from Zalar et al. 2007). A–D. Colony surface grown on PDA (A), OA (B), MEA (C) and MEA plus 5 % NaCl (D) of strains incubated
for 14 d at 25 ºC in darkness. E–F. Habit of conidiophores. G. Conidiophore. H–I. Secondary ramoconidia and conidia. E–I. All from 7-d-old SNA slide cultures. A–D, G, from
CBS 119417 (ex-type strain); E–F, H–I, from EXF-466. Scale bars = 10 (H–I), 30 (G), 50 (F), 100 (E) µm.
Colonies on OA reaching 30–43 mm diam, pale olivaceous to
brownish, reverse iron-grey, velvety to powdery, margin regular,
aerial mycelium sparse, growth lat with papillate surface, without
prominent exudates, sporulation profuse. Colonies on MEA
reaching 30–42 mm diam, pale green, radially furrowed, with
raised, crater-shaped central part, with white, undulate, submerged
margin. Sporulation poor. Colonies on MEA with 5 % NaCl reaching
35–45 mm diam, pale green, velvety, lat with regular margin.
www.studiesinmycology.org
Reverse pale green. Sporulation poor. Maximum tolerated salt
concentration: 20 % NaCl after 14 d. Cardinal temperatures:
Minimum at 10 °C (9 mm diam), optimum at 25 °C (30–42 mm
diam) and maximum at 30 °C (5–18 mm diam).
Substrates and distribution: Hypersaline water and bamboo;
Europe (Slovenia), Asia (India).
285
BenSch et al.
Additional specimen examined: Slovenia, Sečovlje salterns, isolated from
hypersaline water (EXF-466, 471).
Notes: Cladosporium velox is a species of the C. sphaerospermum
complex. The small terminal conidia are, however, more ovoid and
almost smooth (light microscopy).
163. Cladosporium verrucocladosporioides Bensch, H.D. Shin, Crous & U. Braun, Stud. Mycol. 67: 83. 2010. Figs
335–337.
Holotype: South Korea, Hongcheon, N37º48’17’’ E127º51’13’’, isol.
from leaves of Rhus javanica (= R. chinensis) (Anacardiaceae), 11
Sep. 2005, coll. H.-D. Shin, isol. P.W. Crous as “Pseudocercospora
rhoina” (CBS H-20450). Ex-type culture: CBS 126363 = CPC
12300.
Ill.: Bensch et al. (2010: 84−86, igs 72−74).
In vitro: Mycelium immersed and supericial; loosely branched,
1−4.5 μm wide, septate, mostly not constricted at septa, sometimes
distinctly constricted and due to swellings and intercalary constrictions
irregular in outline, subhyaline to pale or medium olivaceous-brown,
smooth to minutely verruculose or verruculose, walls unthickened
or almost so, at the base of conidiophores sometimes wider, up to
5 μm, sometimes anastomosing and forming ropes of few hyphae.
Conidiophores macro-, sometimes also micronematous, arising
terminally and laterally from hyphae, solitary, erect, straight to
lexuous, cylindrical-oblong, sometimes once distinctly geniculatesinuous, non-nodulose, unbranched, 18−130(−175) × (2.5−)3−4
Fig. 335. Cladosporium verrucocladosporioides (CBS 126363). Macro- and
micronematous conidiophores, mycelium sometimes formed in ropes, ramoconidia
and conidial chains in vitro. Scale bar = 10 µm. K. Bensch del.
μm, pluriseptate, septa often in short succession and darkened,
especially just below potential ramoconidia, sometimes distinctly
Fig. 336. Cladosporium verrucocladosporioides (CBS 126363). A–H. Macronematous conidiophores and conidial chains. Scale bars = 10 µm.
286
the genuS Cladosporium
Fig. 337. Cladosporium verrucocladosporioides (CBS 126363). A. CryoSEM of a small colony illustrating the dense middle part with many young conidiophores and some
disconnected masses of conidia. B. Stout erect conidiophores sprouting from rounded linearly oriented cells. C, G–H. Details of the fungal colony containing the structures on
which conidiophores are formed and the different types of conidia. Note the ornamented conidia and the more or less smooth or only slightly ornamented conidiophores. D.
Conidiophore, secondary ramoconidia and scars. E. Rounded conidia in a chain showing the reticulate surface ornamentation. F. Secondary ramoconidium and scars. Note the
reduced ornamentation on this cell in comparison with the conidia. Scale bars = 2 (D, F), 5 (C, E), 10 (G–H), 20 (B), 50 (A) µm.
constricted at septa, pale to medium olivaceous-brown, smooth to
verruculose-verrucose or irregularly rough-walled, at the apex or the
whole conidiophore with surface ornamentation, walls only slightly
thickened, base often somewhat swollen, up to 5(−7) μm wide,
sometimes slightly attenuated towards the apex. Conidiogenous cells
integrated, mostly terminal, sometimes also intercalary, cylindricaloblong, sometimes once geniculate, 7−30 μm long, with 1−3(−4)
loci at the apex, occasionally up to eight loci crowded at the apex,
sometimes situated on small lateral prolongations, subdenticulate,
protuberant, 1−1.8 μm diam, thickened and darkened-refractive.
Ramoconidia occasionally formed, cylindrical-oblong, 19−36(−45)
× 3−4(−5) μm, 0−2(−3)-septate, not constricted at septa, base
unthickened, broadly truncate, 2−3.5 μm wide. Conidia catenate, in
branched chains, branching in all directions, up to 4(−5) conidia in the
unbranched terminal part of the chain, small terminal conidia obovoid,
subglobose, 3−6.5(−7.5) × 2.5−4.5(−5.5) μm (av. ± SD: 5.0 ± 1.4 ×
3.4 ± 1.0), aseptate, rarely 1-septate, apex usually broadly rounded,
intercalary conidia ovoid, limoniform to ellipsoid-ovoid, 6−13.5(−15)
× 3−6 μm (av. ± SD: 9.5 ± 3.0 × 4.2 ± 0.8), 0−1(−2)-septate, not
constricted at septa, with 1−3 distal hila, apex and base often
rounded or attenuated, secondary ramoconidia ellipsoid-ovoid,
subcylindrical to cylindrical-oblong or somewhat irregular, (7−)8.5−30
× (2.8−)3−4(−5) μm (av. ± SD: 18.9 ± 6.0 × 3.7 ± 0.5), 0−3-septate,
not constricted at septa, with 2−6 distal hila crowded at the apex and
www.studiesinmycology.org
situated on small lateral prolongations at the apex giving conidia an
irregular appearance, younger ones pale olivaceous, later usually
medium, sometimes dark olivaceous-brown, almost smooth (younger
conidia) to mostly irregularly rough-walled, surface ornamentation
variable, coarsely verruculose-rugose to verrucose (LM), irregular in
outline, coarse verrucae up to 1 μm high, sometimes outer wall with
surface ornamentation seemingly detaching, under SEM surface with
irregularly reticulate structure or embossed stripes probably caused
by diminishing turgor and shriveling of tender conidia, walls almost
unthickened or often appear to be distinctly thickened, up to 1 μm wide,
hila more or less conspicuous (sometimes not very conspicuous due
to surface ornamentation), subdenticulate to denticulate, 0.5−1.8 μm
diam, thickened and darkened-refractive; sometimes germinating,
occasionally microcyclic conidiogenesis occurring.
Culture characteristics: Colonies on PDA attaining 47−57 mm diam
after 14 d, olivaceous-grey to iron-grey, reverse olivaceous-black,
grey-olivaceous towards margins, felty-loccose to luffy, margin
white, regular, glabrous, aerial mycelium abundant, loose to dense,
without prominent exudates, sporulation profuse. Colonies on
MEA reaching 52−73 mm diam after 14 d, pale olivaceous-grey to
olivaceous-grey or whitish, reverse iron-grey, velvety to loccose,
margins white, glabrous to feathery, regular, aerial mycelium loose
to dense, low, growth effuse, radially furrowed, with wrinkled and
287
BenSch et al.
folded colony centre, often somewhat immersed, without prominent
exudates, sporulating. Colonies on OA attaining 47−54 mm diam
after 14 d, smoke-grey to grey-olivaceous or olivaceous-grey,
reverse pale mouse-grey to leaden-grey, loccose to luffy-felty,
margin colourless, glabrous, regular, aerial mycelium luffy to feltyloccose, abundant, covering large parts of the colony, growth
effuse to low convex, without exudates, sporulation profuse.
Substrate and distribution: On Rhus javanica; Asia (South Korea).
Notes: Cladosporium verrucocladosporioides is welldistinguished from other Cladosporium species by its unusual
conidial surface ornamentation, which is characterized by being
Verrucocladosporium-like, i.e. coarsely verruculose-rugose. The
genus Verrucocladosporium (Crous et al. 2007b), which is a sister
taxon to Cladosporium s. str., has recently been introduced, but
differs from species of the latter genus in having ± planate, noncoronate conidiogenous loci and hila. There are several other
Cladosporium species with verruculose or irregularly rough-walled
conidia, e.g. C. acalyphae, C. exasperatum and C. pini-ponderosae,
but all of them are phylogenetically (see Bensch et al. 2010, ig. 1,
part b vs. a) as well as morphologically distinct.
164. Cladosporium victorialis (Thüm.) U. Braun & H.D.
Shin, Proc. Komarov Bot. Inst. (St. Petersburg) 20: 101.
1997. Figs 338, 339.
Basionym: Cercospora victorialis Thüm., Hedwigia 21: 172. 1882.
Lectotype (designated by Braun, in Braun & Mel’nik 1997): Russia,
West Siberia, Mt. Kerlygan, on Allium victorialis (Amaryllidaceae),
Martjanov (LE 40451). Isolectotype: LE 40452.
= Cladosporium alliicola H.D. Shin & U. Braun, Korean J. Mycol. 23(2): 141.
1995. [KUS 12597, holotype; isotype: HAL 1533 F].
Lit.: Crous & Braun (2003: 422), Schubert (2005b: 148–149).
Ill.: Shin & Braun (1995: 140–141, igs 1–2), Braun & Mel’nik (1997:
ig. 71), Schubert (2005b: 149, ig. 72, pl. 33, igs A–D).
In vivo: Leaf spots amphigenous, subcircular to irregular, up to 5 cm
wide, without deinite margin, at irst discoloured, yellowish, later
becoming greyish brown, also on inlorescences, causing severe
lower rot. Colonies hypophyllous, loosely scattered, often not
very conspicuous, pale to medium grey-brown, on inlorescences
loosely to densely caespitose, greyish brown, velvety. Mycelium
internal, subcuticular to intraepidermal; hyphae branched, 1–8
μm wide, septate, often slightly constricted at the septa, pale
yellowish to medium brown, smooth, walls somewhat thickened,
forming stromatic hyphal aggregations. Stromata substomatal,
small to moderately large, well-developed, cells somewhat angular,
often cylindrical-oblong, medium brown or yellowish brown,
walls smooth and somewhat thickened. Conidiophores usually
densely fasciculate, 3–20 in a divergent fascicle, arising from
stromata, emerging through stomata, sometimes solitary, formed
as lateral branches of internal hyphae, erect, straight, cylindrical,
often geniculate near the apex, 10–50(–68) × 4–7(–10) μm,
0–1(–2)-septate, yellowish, olivaceous-brown to medium brown,
paler towards the apex, smooth, walls unthickened or only very
slightly thickened. Conidiogenous cells integrated, terminal or
conidiophores often reduced to conidiogenous cells, proliferation
sympodial, 0–3 times mildly geniculate, conidiogenous loci often on
288
Fig. 338. Cladosporium victorialis (HAL 1533 F, isotype of C. alliicola). Fascicle of
conidiophores and conidia in vivo. Scale bar = 10 µm. K. Bensch del.
small lateral shoulders, protuberant, obconically truncate to slightly
convex, 2–3(–4) μm wide, up to 1 μm high, conspicuously thickened
and darkened-refractive. Conidia solitary or in short unbranched
or branched chains, more or less straight, subcylindrical-fusiform
to often cylindrical, 10–45(–68) × (3–)4.5–8(–9) μm, occasionally
up to 180 μm in length, 0–4(–7)-septate, not constricted at the
septa, occasionally slightly constricted at the septa, yellowish,
pale olivaceous to pale olivaceous-brown, smooth or almost so,
walls unthickened or only very slightly thickened, apex rounded or
somewhat attenuated, hila protuberant, often short cylindrical, (1–)
1.5–3(–4) μm wide, 0.5–1 μm high, dome often somewhat higher
than the surrounding rim, thickened and darkened-refractive;
occasionally microcyclic conidiogenesis occurring.
Substrate and distribution: On Allium spp.; Asia – A. victorialis
(= A. ochotense) (Russia, Far East, West Siberia, South Korea).
Additional specimen examined: South Korea, Suwon, on Allium victorialis var.
platyphyllum, 3 Sep. 1993, H.D. Shin (HAL 1533 F, isotype of C. alliicola).
Notes: Cladosporium victorialis differs from C. allii and C. alliicepae in having catenate, smooth, pluriseptate, narrower conidia.
Records of this species from Brazil on Allium cepa and A. sativum
by Mendes et al. (1998) are doubtful and unproven.
165. Cladosporium vignae M.W. Gardner, Phytopathology
15(8): 457. 1925. Figs 340, 341.
Lectotype (designated in Bensch et al. 2010): USA, Indiana,
LaFayette, on Vigna unguiculata (= V. sinensis) (Fabaceae), M.W.
Gardner (BPI 427608). Isolectotype: Sep. 1924, M.W. Gardner (BPI
427604). Topotype: 25 Aug. 1925, M.W. Gardner (BPI 427602).
Authentic strain: CBS 121.25 = ATCC 200933 = MUCL 10110.
Lit.: De Vries (1952: 99), Morgan-Jones & McKemy (1992), McKemy
et al. (1993), Ho et al. (1999: 144), Schubert (2005b: 149–152).
Ill.: Morgan-Jones & McKemy (1992: 13, ig. 1; 15, pl. 1; 17, ig. 2),
Ho et al. (1999: 145, ig. 51), Schubert (2005b: 150, ig. 73, pl. 33,
igs E–F).
the genuS Cladosporium
Fig. 339. Cladosporium victorialis (HAL 1533 F, isotype of C. alliicola). A. Overview. B. Fascicle of conidiophores. C. Conidiophores and conidia. D. Conidia. Scale bar = 10
(B–D), 20 (A) µm.
Fig. 340. Cladosporium vignae (BPI 427608). Conidiophores and conidia in vivo.
Scale bar = 10 µm. K. Bensch del.
In vivo: On pods, peduncles, stems, petioles and leaves, causing
scab, leaf and pod blight, leaf spots amphigenous, irregular in
shape, 1–3 mm wide, with a pale yellowish centre, surrounded by
a more or less broad, irregular, reddish brown margin; on pods at
irst punctiform, subcircular, later effuse, oblong-irregular, up to 6
www.studiesinmycology.org
mm diam, red-brown turning blackish, scab-like, sometimes corky
and cracked, often somewhat raised; on stems and petioles redbrown to dark brown, somewhat sunken, covering large areas
of the host tissue. Colonies caespitose, loose to dense, effuse,
greyish brown to dark brown, velvety, conined to necrotic areas.
Mycelium immersed, subcuticular; hyphae branched, 2–7 μm wide,
septate, subhyaline to pale yellowish brown, smooth or almost so,
often swollen and aggregated, swollen hyphal cells subglobose to
somewhat angular, 7–13 μm wide, yellowish brown, smooth, thickwalled. Stromata lacking. Conidiophores solitary or in small groups,
arising from hyphae or swollen hyphal cells, terminal or lateral, or
from swollen hyphal cells, erumpent through the cuticle, sometimes
emerging through stomata, erect, straight to slightly lexuous,
cylindrical, somewhat geniculate-sinuous, unbranched, rarely
branched near the apex, 12–132 × 3–7(–8) μm, septate, septa not
very conspicuous, pale to medium yellowish brown or almost dark
brown, smooth, with age somewhat asperulate, walls at irst only
slightly thickened, later thickened, sometimes even two-layered,
often wider or somewhat swollen at the base, up to 13 μm wide.
Conidiogenous cells integrated, terminal to intercalary, cylindrical,
10–52 μm long, proliferation sympodial, with a single to numerous,
apically often somewhat crowded conidiogenous loci, protuberant,
more or less subdenticulate, short cylindrical, slightly convex,
1–2 μm diam, thickened, refractive or only somewhat darkened.
Ramoconidia occasionally formed, cylindrical, base truncate,
unthickened, up to 3.5 μm wide. Conidia catenate, frequently in
branched chains, straight, small terminal conidia subglobose to
obovoid, 3–6 × 2–3(–4.5) μm, aseptate, pale brown, intercalary
289
BenSch et al.
Fig. 341. Cladosporium vignae (BPI 427608). A. Conidiophores. B. Conidiophores and conidia. Scale bars = 10 µm.
conidia limoniform, ellipsoid, 5–12 × 3–5 μm, 0(–1)-septate, pale
yellowish brown, secondary ramoconidia ellipsoid, cylindrical, 10–
22(–29) × 3–6 μm, 0–1(–2)-septate, not constricted at the septa,
pale yellowish brown, smooth, rarely minutely verruculose, walls
unthickened or slightly thickened, often somewhat attenuated
towards the apex and base, hila truncate to slightly convex, 0.5–2
μm diam, thickened, darkened-refractive; occasionally microcyclic
conidiogenesis occurring.
In vitro (MEA, Ho et al. 1999): Colonies attaining 65 mm diam at
25 °C in 2 wk, pale olivaceous-grey, locculose, velvety, reverse
dark brick. Hyphae septate, pale olive, smooth or verruculose,
(2.5–)2.9–3.7(–4.3) μm wide. Conidiophores pale to mid-olivaceous
or olivaceous, straight or slightly lexuous, usually simple, smooth
or sometimes verruculose towards the base, septate, up to 250
μm long, usually 75–135 μm, (3.3–)3.6–4.1(–5.0) μm wide.
Conidiogenous cells integrated, usually terminal, with limited
sympodial growth, with several conidiogenous loci. Ramoconidia
present. Conidia in branched chains, subglobose, limoniform,
fusiform, ellipsoid, oblong, light olive to olive, 0–1-septate, smooth,
with prominent, protuberant, dark hila. Terminal conidia and
intercalary conidia (6–)7.4–11.1(–20.6) × (2.5–)3.3–3.9(–4.6) μm.
Ramoconidia and secondary ramoconidia (6.3–)10.6–18.8(–23.8)
× (3.1–)3.7–4.2(–4.8) μm, 1–2(–3)-septate.
Substrates and distribution: On Lespedeza and Vigna spp.;
widely distributed, Africa (South Africa, Zimbabwe), Asia (China),
Australasia (Australia), North America (USA), South America
(Brazil) – Lespedeza bicolor (USA, AL), Lespedeza thunbergii
(USA, AL), Vigna unguiculata (Australia, NSW, Queensland; China,
Henan; South Africa; USA, AL, CA, FL, GA, IN, MD, MS, NC, TX),
V. unguiculata ssp. cylindrica (= V. catjang) (Brazil), V. unguiculata
ssp. dekindtiana (Zimbabwe), V. unguiculata ssp. sesquipedalis
(USA, IN).
Additional specimens examined: USA, Alabama, Grady, on Vigna unguiculata, 13
Jul. 1926, S.A. Wingard (BPI 427603); near Oneonta, on Vigna unguiculata, Jul.
1926, L.E. Miles (BPI 427607); Florida, Fort Meade, on Vigna unguiculata, 25 May
1938, K.O. Varn (BPI 427605); Gainesville, on Vigna unguiculata, 1 Jun. 1935,
Erdman West. (NY); Wauchula, on Vigna unguiculata, 23 Apr. 1935, G.F. Weber
(NY); Maryland, Beltsville, on Vigna unguiculata, 25 Apr. 1949, C.L. Lefebvre
(BPI 427606); Mississippi, Nesly, on Vigna unguiculata, 26 Jun. 1930, Fungi of
Mississippi 1386 (NY); North Carolina, Chadburn, on Vigna unguiculata, 6 Jul. 1934,
G.A. Mackstroth (BPI 427609).
290
Notes: This species, which is a seed-borne parasite, is the causal
agent of scab, leaf and pod blight on Vigna unguiculata and
Lespedeza bicolor. Gardner (1925), who introduced this species,
stated that only young growing tissues are susceptible. Inoculation
experiments were carried out to prove the pathogenicity of
C. vignae. Under favourable conditions infections occurred with
great rapidity and virulence, and visible lesions already causing
crinkling of the leaves may be present within 48 h of inoculation.
Attempts to infect ield pea seedlings (Pisum sativum) with the
cowpea fungus have been unsuccessful. Records of C. vignae on
Pisum spp. (e.g., Winstead et al. 1960) are, therefore, doubtful and
probably misidentiications. Da Silva & Minter (1995) recorded this
species from Brazil on Vigna unguiculata subsp. cylindrica [= V.
catjang (“Vigna cajanga”)]. Ogundero (1986) recorded “C. vignae”
as pathogen on grains of Sorghum guineense from Nigeria, which
is very doubtful and probably wrong.
De Vries (1952) examined an isolate of C. vignae sent to the
CBS by M.W. Gardner in 1925, but found sporulation to be poor. On
the basis of what could be observed, he concluded that this species
was similar to C. cladosporioides and that it would probably have
to be considered as a forma specialis of that species once better
isolates were studied. The same isolate examined by de Vries is
still preserved in the CBS culture collection but forms only sterile
mycelium. Morgan-Jones & McKemy (1992) and Ho et al. (1999)
examined C. vignae in culture, provided detailed descriptions of
its features in vitro and discussed its morphological similarity
with C. cladosporioides treating them as two separate species.
Besides its pathogenicity to Vigna and Lespedeza spp. and its
very characteristic symptoms, C. vignae is distinguished from C.
cladosporioides in having somewhat wider conidiophores with
several to numerous often somewhat crowded conidiogenous loci.
Cladosporium cucumerinum, causal agent of crown blight and scab
or gummosis disease of Cucurbitaceae, is morphologically also
close to C. vignae but separated by its mostly longer conidiophores,
its somewhat longer and wider ramoconidia and its immersed
hyphae often possessing a slime coat. Phylogenetically C. vignae
is quite distinct from the morphologically similar C. cladosporioides
and C. cucumerinum (see Bensch et al. 2010, ig. 1, part c).
Cladosporium psoraleae on Cullen corylifolium [≡ Psoralea
corylifolia] (Fabaceae, tribus Phoraleae) is tentatively maintained
as a separate species since the conidiogenous loci are somewhat
wider and the conidia are usually somewhat longer and wider,
subglobose and obovoid terminal conidia are usually lacking
the genuS Cladosporium
(Schubert 2005b). Additional collections, cultures and molecular
data are needed to clarify whether this species is distinct from C.
vignae or not.
166. Cladosporium vincicola U. Braun & K. Schub.,
Mycotaxon 103: 209. 2008. Fig. 342.
≡ Cladosporium vincae Moesz, Bot. Közlem. 23: 123. 1926, nom. illeg.,
homonym, non C. vincae Fairm., 1911.
Lectotype (designated by Braun et al. 2008a): Hungary, near
Budapest, on living leaves of Vinca herbacea (Apocynaceae)
infected by Puccinia vincae (DC.) Plowr., 25 Apr. 1926, W. Moesz
(W 10216).
Ill.: Braun et al. (2008a: 210, ig. 1).
In vivo: Leaf spots usually terminally or laterally initiated, later
spreading, covering large leaf segments, often more than 50 %
of the leaf blade, inally entire leaves turning necrotic, shape and
size of the lesions variable, dark brown to pale grayish brown, later
dingy gray to grayish white, margin indeinite or with a narrow to
moderately wide dark brown to blackish border or halo, sometimes
with rather diffuse discolorations. Colonies amphigenous, mainly
hypophyllous, punctiform, scattered, dark brown. Mycelium
internal, forming immersed stromatic aggregations, 10–80 μm
diam, composed of swollen hyphal cells, pigmented, thick-walled,
up to 12 μm diam. Conidiophores in small to moderately large
fascicles, loose to moderately dense, occasionally solitary, arising
from substomatal stromatic hyphal aggregations, emerging through
stomata, erect, straight, subcylindrical to moderately geniculatesinuous, rarely subnodulose, unbranched, 15–100 × 3–7.5 μm, at
the very base sometimes up to 10 μm wide, 0–4(–6)-septate, pale
to medium olivaceous or olivaceous-brown, wall 0.5–1 μm thick,
one-layered, smooth to faintly rough-walled. Conidiogenous cells
integrated, terminal or intercalary, 10–35 μm long, conidiogenous
loci distinctly coronate, somewhat protuberant, 1.5–2.5 μm
wide and ca. 1 μm high. Ramoconidia lacking. Conidia in simple
or branched chains, ellipsoid-ovoid, obovoid, fusiform, rarely
subcylindrical, (4.5–)6–25(–33) × (3–)4–8(–9) μm, 0–3(–4)-septate,
pale olivaceous to olivaceous-brown, wall thin, ca. 0.5 μm,
sometimes distinctly two-layered and up to 1 μm thick, distinctly
verruculose, apex rounded in conidia formed singly, attenuated
in catenate conidia, base rounded, with an abruptly protuberant
hilum or attenuated, 1–2 μm diam, occasionally with microcyclic
conidiogenesis.
Substrates and distribution: On Vinca spp.; Europe – Vinca
herbacea (Hungary), V. minor (Russia).
Additional specimens examined: Russia, St. Petersburg, Botanical Garden of the
Komarov Botanical Institute, on living leaves of Vinca minor, 7 May 2007, V.A.
Mel’nik (HAL 2069 F, LE 232293).
Notes: Morphologically this species resembles C. aecidiicola.
However, C. vincicola is undoubtedly biotrophic, forming distinct
leaf lesions, with fasciculate conidiophores emerging through
stomata, and is not associated with rust aecia. The fascicles of
conidiophores arise from well-developed, large stromata. The
conidia of C. vincicola agree well with those of C. herbarum s. str.,
but the conidiophores and the arrangement of the conidiogenous loci
www.studiesinmycology.org
Fig. 342. Cladosporium vincicola (W 10216). Fascicle of conidiophores emerging
through stomata, conidiophores, conidiogenous cells and conidia in vivo. Scale bar
= 10 µm. U. Braun del.
are different. The conidiophores in C. vincicola are non-nodulose,
i.e., the conidiogenous loci are not conined to nodulose swellings
as in C. herbarum. Cladosporium vincae Fairm. is identical with C.
macrocarpum, i.e., the conidiophores are distinctly nodulose with
conidiogenous loci conined to swellings, and the conidia are rather
broad, 5.5–11 μm, mostly 8–10 μm.
167. Cladosporium xylophilum Bensch, Shabunin, Crous
& U. Braun, Stud. Mycol. 67: 87. 2010. Figs 343–345.
Holotype: Russia, Leningrad Oblast, Vyborg District, Roshchino,
Pionerskoje Forestry, isol. from dead wood of Picea abies
(Pinaceae), 2005, D.A. Shabunin (CBS H-20452). Ex-type cultures:
CBS 125997 = CPC 12403.
= ? Cladosporium indigoferae Sawada, Special Publ. Coll. Agric. Natl. Taiwan
Univ. 8: 196. 1959, nom. inval. [BPI 427230, PPMH, syntypes], syn. nov.
Lit.: Braun et al. (2008a: 214–215).
Ill.: Sawada (1959: pl. 3, igs 8–9), Braun et al. (2008a: 215, ig. 4),
Bensch et al. (2010: 88, igs 75–76).
In vitro: Mycelium immersed and supericial; hyphae unbranched or
loosely branched, (0.5−)1−4(−5) μm wide, septate, not constricted at
291
BenSch et al.
Fig. 343. Cladosporium xylophilum (BPI 427230, PPMH, syntypes of C. indigoferae).
Conidiophores and conidia in vivo. Scale bar = 10 µm. K. Bensch del.
Fig. 344. Cladosporium xylophilum (CBS 125997). Macro- and micronematous
conidiophores, mycelium sometimes formed in ropes and conidial chains in vitro.
Scale bar = 10 µm. K. Bensch del.
septa, sometimes with irregular swellings and outgrowths, subhyaline
to pale or medium olivaceous-brown, smooth to asperulate, minutely
verruculose or irregularly verrucose and rough-walled, sometimes
with wart-like structures on the surface, walls unthickened or
almost so, occasionally swollen at the base of conidiophores, up
to 8 μm wide, sometimes forming ropes or rhizoids, branched at
the base of conidiophores. Conidiophores macro-, semimacro- to
sometimes micronematous, solitary, arising terminally and laterally
from hyphae, erect, straight to slightly lexuous, cylindrical-oblong,
usually neither nodulose nor geniculate, sometimes subnodulose
at the uppermost apex, occasionally once geniculate-sinuous,
unbranched, sometimes once branched, 7−155(−190) × 2−4(−5)
μm, 0−6-septate, sometimes slightly constricted at septa, pale
to medium olivaceous-brown, smooth or almost so, sometimes
somewhat irregularly rough-walled or verruculose, especially
292
towards the base, sometimes wider at the base, up to 5.5 μm
wide, or slightly attenuated towards the apex, walls unthickened or
slightly thickened; micronematous conidiophores paler, subhyaline
to pale olivaceous-brown, smooth or almost so. Conidiogenous
cells integrated, usually terminal, cylindrical-oblong, usually neither
nodulose nor geniculate, sometimes subnodulose at the uppermost
apex with loci situated on small lateral shoulders due to sympodial
proliferation, 6−36 μm long, with (1−)2−4(−6) apically crowded
loci forming clusters of pronounced scars, sometimes with few
additional loci at a slightly lower level, protuberant, subdenticulate
to denticulate, (0.8−)1−2 μm diam, thickened and darkenedrefractive. Ramoconidia occasionally formed, cylindrical-oblong,
19−35 μm long, 0(−1)-septate, smooth, base broadly truncate,
2.5−3 μm wide. Conidia numerous, catenate in densely branched
chains, branching in all directions, mostly 2−4(−5) conidia in the
unbranched terminal part of the chains, straight, small terminal
conidia subglobose, obovoid, sometimes globose, 2−5(−6) × 2−2.5
μm (av. ± SD: 3.9 ± 0.9 × 2.3 ± 0.3), aseptate, slightly attenuated
towards apex and base, apex broadly rounded, intercalary conidia
ovoid, limoniform to ellipsoid or subcylindrical, sometimes irregular
in outline especially towards the distal end due to numerous hila
arranged in sympodial clusters of pronounced scars, 5−11(−13)
× (2−)2.5−3 μm (av. ± SD: 7.7 ± 2.2 × 2.6 ± 0.3), 0−1-septate,
septum median, not constricted, with 2−7(−10) distal hila, crowded
at the apex, sometimes situated on small lateral prolongations,
small terminal conidia and intercalary conidia almost smooth to
often irregularly rough-walled, loosely verruculose to verrucose,
attenuated towards apex and base, secondary ramoconidia
ellipsoid, subcylindrical to cylindrical-oblong or irregular in outline,
(5.5−)7−23(−32) × (2−)2.5−4(−5) μm (av. ± SD: 14.5 ± 5.1 × 3.1 ±
0.5), 0−1(−3)-septate, septum median or somewhat in the upper
half, not constricted, with (2−)3−7(−10) distal hila, crowded at the
apex or situated on small lateral prolongations, pale olivaceous
to pale medium olivaceous-brown, smooth or almost so, walls
unthickened or almost so, hila conspicuous, subdenticulate to
denticulate, 0.5−2 μm diam, somewhat thickened and darkenedrefractive; microcyclic conidiogenesis occasionally occurring with
conidia forming secondary conidiophores.
Culture characteristics: Colonies on PDA attaining 52−74 mm diam
after 14 d, olivaceous-grey to grey-olivaceous, reverse iron-grey
to olivaceous-black, loccose to luffy, margins grey-olivaceous,
feathery, aerial mycelium abundant, loccose to luffy, loose to
dense, growth effuse, without exudates, sporulation profuse.
Colonies on MEA reaching 47−74 mm diam after 14 d, olivaceousgrey, whitish due to aerial mycelium, reverse olivaceous to irongrey, velvety to loccose-felty, margins feathery, aerial mycelium
felty, whitish to pale olivaceous-grey, loose to dense, growth effuse
with sometimes papillate surface, sometimes with numerous small
prominent exudates, sporulation profuse. Colonies on OA reaching
47−58 mm diam after 14 d, pale olivaceous-grey to olivaceousgrey, olivaceous-buff, greenish olivaceous to grey-olivaceous due
to sporulation, reverse pale olivaceous-grey to olivaceous-grey,
velvety, loccose to luffy-felty, margins grey-olivaceous, glabrous
to feathery, regular, aerial mycelium abundant, luffy to loccose,
felty, low to high, loose to dense, growth effuse, without exudates,
sporulation profuse.
Substrates and distribution: On wood and plant material; Asia
(Taiwan), Europe (France, Italy, Russia), North America (Canada,
USA).
the genuS Cladosporium
Fig. 345. Cladosporium xylophilum (CBS 125997). A–G. Macronematous conidiophores and conidial chains in vitro. Scale bar = 10 µm.
Additional specimens examined: Canada, Ontario, isol. from galls of Apiosporina
morbosa (Venturiaceae) on twigs of Prunus sp. (Rosaceae), 2005, coll. K.A.
Seifert, isol. P.W. Crous (CPC 12101). France, caves de Madelaine, isol. from
leaves, 21 Aug. 2007, P.W. Crous (CPC 14281). Italy, isol. from twigs of Salix
viminalis (Salicaceae), Sep. 2006, coll. W. Gams, isol. P.W. Crous (CBS 126588 =
CPC 13512, CPC 13513, 13514). ?Taiwan, Pref. Taipei, Taipei, on dead stems of
Indigofera tinctoria (Fabaceae), 18 Aug. 1909, K. Sawada (BPI 427230, syntype
of C. indigoferae); 18 Aug. 1942, K. Sawada (PPMH, authentic material of C.
indigoferae). USA, Washington, isol. from bing cherry fruits (Rosaceae), isol. by
F.M. Dugan (CBS 113749 = cv 10-53 sci1, CBS 113756 = st5-25 sci 1).
Notes: Cladosporium xylophilum, which inhabits woody plants,
belongs to the C. cladosporioides complex. Strains of this
species formed a separate subclade in phylogenetic analyses
(see Bensch et al. 2010, ig. 1, part b). Based on the formation of
numerous subglobose, globose to broadly ovoid, verruculose or
verrucose terminal conidia, this species seems to be allied to C.
sphaerospermum, but differs in having usually 0–1(–2)-septate
ramoconidia and conidia. Furthermore, the two species are
genetically quite distinct, i.e., C. xylophilum does not cluser within
the C. sphaerospermum complex. Strain CPC 14364, isolated
from indoor air in Denmark, clustered with other isolates of C.
xylophilum but differs in having smooth terminal and intercalary
conidia, shorter secondary ramoconidia (6–17(–19) μm) and
slightly narrower conidiogenous loci and hila (0.5–1.5(–1.8) μm
diam). Based on ACT sequence data this single strain clusters
outside the C. xylophilum subclade, but with TEF data it sits within
these isolates (see Bensch et al. 2010, ig. 1, part b). Strains of
this species are in general characterised by a certain degree of
intraspeciic variation. However, the question if cryptic species
are involved cannot yet be answered, but must be based on a
broader sampling.
www.studiesinmycology.org
Cladosporium indigoferae is an invalid name, published without
Latin description. The type material examined is rather sparse, but
some fruiting has been found. In Braun et al. (2008a) this species
was excluded from the genus Cladosporium and its morphological
similarity with the features of Fusicladium and Cladophialophora
was discussed. However, SEM photographs of conidiophores and
conidia from type material showed typical cladosporioid scars with
a central dome and raised rim (not shown here, please see www.
mycobank.org). Therefore, the species has to be treated as a true
member of the genus Cladosporium. Morphologically it agrees with
the recently introduced species C. xylophilum. Both species are
described from dead wood. Conidiophores of C. indigoferae are
15–100(–145) × 3–4.5(–5) μm, the smooth and 0–1(–3)-septate
conidia are 4–26 × 3–5(–7) μm and conidiogenous loci and hila
1–2 μm diam. However, the material examined is in too poor
condition for a inal treatment, which should be based on culture
characteristics supported by molecular DNA sequence analyses.
168. Cladosporium xyridis Tracy & Earle, Bull. Torrey Bot.
Club 23(5): 206. 1896, as “Gladisporium (sic) xyridis”. Figs
346, 347.
Lectotype (designated here): USA, Mississippi, Ocean Springs,
on petals of Xyris imbriata (Xyridaceae), 29 Sep. 1895, F.S. Earle
(NY). Isolectotypes: BPI 427627–427628, NY.
Lit.: Saccardo (1899: 1081), Schubert (2005b: 152–154).
Ill.: Schubert (2005b: 153, ig. 74, pl. 33, igs G–H).
293
BenSch et al.
25–275 × 2–4.5 μm, slightly swollen at the base, up to 6 μm wide,
pluriseptate, pale to medium brown, often paler towards the apex,
sometimes subhyaline at the apex, smooth or almost so, often faintly
verruculose near the base, thick-walled, 0.5–1 μm thick, often more
or less distinctly two-layered, protoplasm sometimes aggregated at
the septa, appearing to be thickened, sporadically enteroblastically
proliferating. Conidiogenous cells integrated, terminal and
intercalary, narrowly cylindrical-oblong, sometimes somewhat
geniculate-sinuous, 10–48 μm long, proliferation sympodial, with a
single or several conidiogenous loci, often somewhat crowded at the
same level, protuberant, subdenticulate, short cylindrical, 1–1.5(–
2) μm diam, thickened, somewhat darkened-refractive. Conidia
catenate, in branched chains, more or less straight, subglobose,
obovoid, ellipsoid, somewhat fusiform, subcylindrical to cylindrical
or irregular, 3.5–23(–31) × 2–5(–7) μm, 0–2(–3)-septate, not or only
slightly constricted at the septa, subhyaline or even hyaline to very
pale brown, smooth or almost so, walls more or less thickened,
apex rounded or often slightly attenuated towards apex and base,
apex often with 1–5 hila, protuberant, 0.5–1.5(–2) μm diam, clearly
differentiated in a central dome and a periclinal rim, thickened,
somewhat darkened-refractive; microcyclic conidiogenesis often
occurring.
Substrates and distribution: On Xyris spp.; Caribbean, North
America – Xyris imbriata (USA, MS), X. jupicai (Cuba), Xyris spp.
(USA, AL).
Fig. 346. Cladosporium xyridis (NY). Conidiophores and conidia in vivo. Scale bar
= 10 µm. K. Bensch del.
Additional specimens examined: Cuba, Herradura, on Xyris jupicai (= X.
communis), 30 Oct. 1917, F.S. Earle No. 803 (NY). USA, Alabama, Tuskegee (?),
on Xyris sp., 12 Jul. 1897, G.W. Carren (?) (NY); Lee Co., Auburn, on Xyris sp., 8
Sep. 1897, F.S. Earle & C.F. Baker (NY).
In vivo: Blackening the persistent withering petals. Colonies
stretching petals, effuse, loosely caespitose, pale brown, villose.
Mycelium internal, subcuticular to often intraepidermal; hyphae
branched, 2.5–9 μm wide, septate, often with swellings and
constrictions, subhyaline or even hyaline to pale medium brown,
smooth, walls thickened, cells occasionally with a distinct lumen
clearly separated from the thickened wall, often appearing to be
granular, hyphae forming a loose network, sometimes aggregated.
Stromata lacking. Conidiophores solitary or arranged in small
loose groups, arising from internal hyphae, erumpent, erect, more
or less straight or only very slightly lexuous, iliform, sometimes
slightly geniculate-sinuous, mostly non-nodulose, unbranched,
Notes: Branched conidiophores as described in the original
diagnosis (Tracy & Earle 1896) could not be observed in the
collections examined. On the web site of the herbarium BPI the
name of this species was given as “hyridis”, which is very probably
a spelling error.
Cladosporium xyridis is the only species known to occur on
a host belonging to the Xyridaceae. It could morphologically be
compared with C. uredinicola and C. cladosporioides but the
latter species differs, however, in having wider conidiogenous
loci and hila, usually 0–1-septate conidia and conidiophores
with a single or only few conidiogenous loci. The conidia of the
fungicolous C. uredinicola are often much longer and somewhat
wider, 3–39 × 2–6.5(–8) μm, the walls of the conidiophores are
Fig. 347. Cladosporium xyridis (NY). A–B. Conidiophores, conidia and microcyclic conidiogenesis in vivo. Scale bars = 10 µm.
294
the genuS Cladosporium
one-layered and somewhat narrower, the conidiogenous loci
are somewhat wider, and sometimes ramoconidia are formed
(Heuchert et al. 2005).
169. Cladosporium yuccae U. Braun, Schlechtendalia 19:
65. 2009. Fig. 348.
Holotype: Italy, South Tyrol, Neumarkt, Fontane frede, on faded
leaves oft Yucca sp. (Asparagaceae), 8 Sep. 1902, P. Magnus
(HBG).
Ill.: Braun (2009: 66, ig. 2).
In vivo: Colonies on large necrotic lesions, punctiform, scattered
to gregarious, darl brown to blackish. Mycelium internal. Stromatic
hyphal aggregations immersed, 10–100 μm diam, occasionally
conluent, dark brown, composed of swollen hyphal cells, 2–8
μm diam. Conidiophores in small to very large, loose to dense
fascicles, arising from stromatic hyphal aggregations, erumpent,
erect, straight, subcylindrical to usually strongly irregularly
sinuous to subnodulose by constrictions at the septa, occasionally
geniculate, simple or often branched, 20–80 × 3–8 μm, pluriseptate
throughout, pale to medium dark brown or olivaceous-brown, often
paler towards the apex, tips occasionally very pale or subhyaline,
smooth or almost so, wall thin, ≤ 1 μm, conidiophores occasionally
with monopodial rejuvenation leaving simple annellate structures.
Conidiogenous cells integrated, terminal and intercalary, 10–20 μm
long, subcylindrical to geniculate, with a single to usually several,
often crowded conidiogenous loci, distinctly coronate, but only
slightly protuberant, (1–)1.5–2 μm diam, somewhat darkenedrefractive. Ramoconidia occasionally formed, base truncate,
2–2.5(–3) μm wide, without coronate hilum. Conidia catenate, in
branched acropetal chains, ellipsoid-ovoid, obovoid, fusiform, 4–18
× 3–7 μm, 0–1-septate, pale olivaceous to olivaceous-brown, thinwalled, almost smooth to usually distinctly, but minutely verruculose,
ends rounded to somewhat attenuated, small terminal conidia pale,
4–8 × 3–4 μm, septate conidia and secondary ramoconidia 10–18
× 4–7 μm, hila somewhat protuberant, distinctly coronate, 1–2 μm
diam, refractive; microcyclic conidiogenesis not occurring.
www.studiesinmycology.org
Fig. 348. Cladosporium yuccae (HBG). Fascicles of conidiophores, tips of
conidiophores, ramoconidia and conidia. Scale bar = 10 µm. U. Braun del.
Substrates and distribution: On Yucca; Italy.
Notes: This species is morphologically close to C. chamaeropis,
but the latter species differs from C. yuccae in having usually
unbranched conidiophores without distinct constrictions and
longer conidiogenous cells, up to 35 μm in length. Furthermore,
ramoconidia have not been observed in C. chamaeropis. The
ecology of C. yuccae is unclear and cannot be veriied on the base
on the old herbarium material. The colonies of C. yuccae occur on
large necrotic lesions, but it is unclear if they have been caused by
the latter fungus. New fresh collections are needed to elucidate the
true biology of this species.
295
BenSch et al.
EXCLUDED TAXA OF CLADOSPORIUM S. STR.
Species and other taxa excluded from Cladosporium s. str. are
listed alphabetically. In the irst line of each entry, the particular
epithets used under Cladosporium are given, but the generic name
Cladosporium is dropped. As far as known, synonyms are given,
including the currently accepted name, which is marked in bold.
References to type collections are given for the particular taxa and
their synonyms. Furthermore, important literature references (Lit.),
above all those dealing with nomenclature and taxonomy, and
selected illustrations (Ill.) are cited.
Cladosporium
abietinum (Pers.) Link, Ges. Naturf. Freunde Berlin Mag.
Neuesten Entdeck. Gesammten Naturk. 7: 37. 1816.
Type: On bark of Pinus abies (Pinaceae).
Basionym: Dematium abietinum Pers., Neues Mag. Bot. 1: 121.
1794.
≡ Sporotrichum abietinum (Pers.) Link, Ges. Naturf. Freunde Berlin Mag.
Neuesten Entdeck. Gesammten Naturk. 3: 13. 1809.
Lit.: de Vries (1952: 89).
Notes: According to Hughes (1958), this species has to be
reduced to synonymy with Trentepohlia abietina (Flot.) Hansg.
Hughes examined material deposited at B (No. 700006134) as
“Cladosporium abietinum Link” (label without any further details). It
is not clear if this material was part of Link’s herbarium.
abietinum Zukal, Verh. K. K. Zool.-Bot. Ges. Wien 37: 44–45.
1887, nom. illeg., homonym, non C. abietinum (Pers.) Link,
1816.
Type: Austria, on moist tuber skins of Solanum tuberosum
(Solanaceae), 1885, associated with Chaetomium crispatum.
≡ Spondylocladium abietinum Sacc., Syll. fung. 10: 662. 1892, as “(Zukal)
Sacc.”.
Lit.: Lindau (1910: 142, as synonym of “Spondylocladium atrovirens
Harz”).
Ill.: Zukal (1887: tab. 1, ig. 8).
Notes: Zukal’s type, originally housed at GZU, could not be located
there (personal communication with Christian Scheuer). Based
on Zukal’s (1887) drawing, this species has to be excluded from
Cladosporium s. str.
acaciae Panwar → acaciicola.
acaciicola M.B. Ellis, More Dematiaceous Hyphomycetes:
342. 1976.
Type: India, Rajastan, Jodhpur, sand dunes of Masuria, from
rhizosphere of Acacia nilotica subsp. indica (Fabaceae) (IMI
104172).
Basionym: Cladosporium acaciae Panwar, Curr. Sci. 39(18): 422.
1970, non C. acaciae Reichert, 1921.
Ill.: Ellis (1976: 343, ig. 260 A).
Notes: Excluded, a black yeast-like fungus.
adianticola R.F. Castañeda, Fungi Cubenses II: 3. 1987, as
“adianticolum”.
Holotype: Cuba, prov. Matanzas, San Miguel de los Baños, on
living leaves of Adiantum sp. (Pteridaceae), 23 Jan. 1987, R.F.
Castañeda (INIFAT C87/40).
Lit.: Crous et al. (2006, 2007c).
296
Fig. 349. Parastenella aequatoriensis (M-0057478, syntype of C. aequatoriense).
Conidiophores and conidia. Scale bar = 10 µm. K. Bensch del.
Ill.: Castañeda (1987: igs 3, 5).
Notes: Excluded, generic afinity not yet settled. Using DNA
sequence data, Crous et al. (2006, 2007c) demonstrated that this
species clusters within the Herpotrichiellaceae, close to species of
the genus Metulocladosporiella.
aequatoriense Petr., Sydowia 2: 380. 1948. Fig. 349.
Syntype: Ecuador, prov. Pichincha, Guarumos near Nono, on
living leaves of Mikania sp. (Asteraceae), 17 Oct. 1937 (M-57478).
≡ Parastenella aequatoriensis (Petr.) K. Schub. & U. Braun, Mycol.
Progr. 4(2): 103. 2005.
Ill.: Schubert & Braun (2005a: 104, ig. 3).
albicans Hallier, Parasitologische Untersuchungen bezüglich
auf die planzlichen Organismen bei Masern, Hungertyphus,
Darmtyphus, Blattern, Kuhpocken, Schafpocken, Cholera
Nostra etc.: 27. 1868.
Type: From man, causing parasitic stomatitis (location unknown).
≡ Cladosporium albicans Hallier, Flora, Neue Reihe, 26(19): 293. 1868,
nom. prov.
Ill.: Hallier (1868b: pl. II, ig. 2).
Notes: Considered to be a state (morph) of Oidium albicans C.P.
Robin [≡ Candida albicans (C.P. Robin) Berkhout] by Hallier
(1868b: 27); human pathogenic, undoubtedly not belonging to
Cladosporium s. str. Hallier (1866: 86) discussed this fungus under
“Stempylium polymophum ? (Oidium albicans auct.)”.
album Dowson, J. Roy. Hort. Soc. 49(2): 211. 1924.
Type: UK, on living leaves of Lathyrus odoratus (Fabaceae).
≡ Hyalodendron album (Dowson) Diddens, Zentralbl. Bakteriol., 2. Abt.,
90: 316. 1934.
≡ Ramularia alba (Dowson) Nannf., in Lundell & Nannfeldt, Fungi Exs.
Suec., Fasc. XXXIX–XL, No. 17. 1950.
= Ramularia galegae f. lathyri Ferraris, Malphigia 20: 153. 1906.
≡ Ramularia lathyri Ferraris, Flora Ital. Crypt., Pars I, Fungi: 812. 1913.
the genuS Cladosporium
≡ Ramularia lathyri (Ferraris) Bubák, Ann. Mycol. 14: 350. 1916.
= Ramularia lathyri Hollós, Bot. Közlem. 1910, 2: 112. 1910.
= Ramularia deusta f. odorati W.C. Snyder & W.H. Davis, Mycologia 42: 417.
1950.
= Ramularia deusta (Fuckel) Karak. var. alba U. Braun, Nova Hedwigia 56:
429. 1993.
Lit.: Braun (1998: 157).
algeriense (Montpell. & Catanei) Vuill., Champ. paras.: 78.
1931.
Type: Isol. from man (location unknown).
Basionym: Hormodendrum algeriense Montpell. & Catanei, Ann.
Dermatol. Syphiligr., Sér. 6, 8: 626. 1927.
Lit.: Dodge (1935: 845), de Hoog et al. (2000: 1014).
Notes: De Hoog et al. (2000) described it as a doubtful species
close to or identical with Fonsecaea pedrosoi (Brumpt) Negroni
[current name Phialophora pedrosoi (Brumpt) Redaelli & Cif.].
alternicoloratum R.F. Castañeda & W.B. Kendr., Univ.
Waterloo Biol. Ser. 35: 20. 1991.
Holotype: Cuba, Pinar del Río, Cuchillas de San Simón, on
leaves and stems of Cyperus alternifolius (= C. involucratus)
(Cyperaceae), 24 Mar. 1990, R.F. Castañeda (INIFAT C90/129).
Ill.: Castañeda & Kendrick (1991: 21, ig. 10).
Notes: Excluded, generic afinity unclear.
americanum H.C. Greene, Amer. Midl. Naturalist 41(3): 723.
1949.
Lectotype (designated here): USA, Wisconsin, Dane Co.,
Madison, University of Wisconsin Arboretum, on living leaves of
Prunus americana (Rosaceae), 18 Sep. 1944, H.C. Greene (WIS).
Isolectotype: BPI 426105.
= Fusicladium carpophilum (Thüm.) Oudem., Verh. Kon. Ned. Akad.
Wetensch., Afd. Natuurk. 1900: 388. 1900.
Lit.: Braun (2001: 53), Schubert (2005a: 56–57).
Ill.: Schubert (2005a: 57, ig. 1).
amoenum R.F. Castañeda, BCCM MUCL Agro-industrial
fungi-yeasts, 1998. nom. nud.
Iconotype: Cuba, Santiago de Cuba, La Gran Piedra, on fallen
leaves of Eucalyptus sp. (Myrtaceae), 2 Nov. 1994, R.F. Castañeda
(Ho et al. 1999: 117, Figs 2–3). Ex-type culture: ATCC 200947,
CBS 254.95, INIFAT C94/155, IMI 367525, MUCL 39143.
≡ Anungitopsis amoena R.F. Castañeda & Dugan, Mycotaxon 72: 118.
1999.
≡ Fusicladium amoenum (R.F. Castañeda & Dugan) Crous, K. Schub. &
U. Braun, Stud. Mycol. 58: 207. 2007.
Ill.: Ho et al. (1999: 117, igs 2–3), Crous et al. (2007d: 206, ig. 20).
Notes: Crous et al. (2007d), using molecular sequence analyses,
showed that Anungitopsis amoena is not congeneric with the type
species of Anungitopsis and has to be transferred to Fusicladium
(Venturiaceae).
amorphae Thüm., Rev. Mycol. (Toulouse) 1: 59. 1879.
Syntypes: USA, South Carolina, Aiken, on dead stems of Amorpha
herbacea (Fabaceae), H.W. Ravenel, Thümen, Mycoth. Univ. 1572
(e.g., B 700006157, BPI 426106, BR-MYC 81368,82, HAL, HBG,
M, NY).
Lit.: Saccardo (1886: 362).
Notes: Excluded species. Status unclear (conidiogenesis probably
tretic). The original description is repeated in Thümen, Mycoth.
Univ., Cent. XVI, No. 1572, 1880.
www.studiesinmycology.org
ampelinum Pass., in Baglietto, Cesati & Notaris, Erb. Critt.
Ital., Ser. II, No. 595. 1872.
Syntypes: Italy, on leaves of Vitis sp. (Vitaceae), Baglietto, Cesati
& Notaris, Erb. Critt. Ital. 595 (e.g., E, IMI 112146).
= Pseudocercospora vitis (Lév.) Speg., Anales Mus. Nac. Buenos Aires 20:
438. 1910.
Lit.: Saccardo (1886: 458), Lindau (1910: 116), Oudemans (1921),
Sivanesan (1984: 210), Crous & Braun (2003: 427).
Notes: See C. vitis (Lév.) Sacc.
anomalum Berk. & M.A. Curtis, J. Linn. Soc., Bot. 10: 362.
1869.
Type: Cuba, on the underside of leaves of a Malvaceae, C. Wright,
Fungi cubensis wrightiani, No. 639 (K).
≡ Pseudocercospora anomala (Berk. & M.A. Curtis) de Hoog,
Persoonia 15(1): 68. 1992.
Lit.: Saccardo (1886: 363).
Notes: Authentic material is also deposited at PC (C. Wright
collection, 1870, North Paciic Expedition 1853–56).
antillanum R.F. Castañeda, Fungi Cubensis II: 3. 1987.
Holotype: Cuba, prov. Guantánamo, Imias, on fallen leaves of
Clusia rosea (Clusiaceae), 3 May 1986, Mayra Camino (INIFAT
C86/128).
Ill.: Castañeda (1987: ig. 6).
Notes: Excluded, no Cladosporium s. str., but generic afinity not
yet clear.
araguatum (Syd.) Arx, Genera Fungi Sporul. Pure Cult., Ed.
2: 224. 1974.
Lectotype (selected by Crous et al. 2007c): Venezuela, Aragua,
La Victoria, between La Victoria and Suata, on living leaves of
Pithecellobium lanceolatum (Fabaceae), Jan. 1928, H. Sydow [IMI
15728(a)]. Isolectotypes: BPI 443420, 443421, 443422, IMI 34905.
≡ Stenella araguata Syd., Ann. Mycol. 28: 205. 1930.
= Cladosporium castellanii Borelli & Marcano, Castellania 1(5): 154. 1973.
[Type: IMI 183818, ex-type culture: CBS 105.75].
Lit.: McGinnis & Padhye (1978), Crous et al. (2007c: 43–45).
Ill.: Crous et al. (2007c: 44–45, igs 7–8).
Notes: This is the type species of the genus Stenella. Crous
et al. (2007c) examined this species in detail, and based on
morphology and DNA sequence data, showed that it clusters in the
Teratosphaeriaceae (Capnodiales).
araliae Sawada, Rep. Gov. Res. Inst. Formosa 85: 91. 1943,
nom. inval. Fig. 350.
Holotype: Taiwan, on Aralia decaisneana (Araliaceae), 26 Nov.
1928, K. Sawada (BPI 426122). Isotype: PPMH.
≡ Stenella araliae Sawada ex K. Schub. & U. Braun, Nova Hedwigia 84:
202. 2007.
≡ Zasmidium araliae (Sawada ex K. Schub. & U. Braun) K. Schub. & U.
Braun, Schlechtendalia 20: 100. 2010.
Ill.: Schubert & Braun (2007: 203, ig. 7).
argillaceum Minoura, J. Ferment. Technol. 44: 140. 1966.
Type: Japan, Yaku, isol. from a decaying myxomycete, 21
Oct. 1961, K. Tubaki (Dept. Fermentation Technology, Faculty
Engineering, Osaka, Japan [IFO]). Ex-type culture: CBS 241.67.
≡ Rhizocladosporium argillaceum (Minoura) Crous & U. Braun, Stud.
Mycol. 58: 50. 2007.
Lit.: Heuchert et al. (2005: 53), Crous et al. (2007c: 50–51).
Ill.: Minoura (1966: 142, ig. 6 E), Crous et al. (2007c: 50, ig. 12).
297
BenSch et al.
Fig. 350. Zasmidium araliae (BPI 426122, holotype of C. araliae). Conidiophores
and conidia. Scale bar = 10 µm. K. Bensch del.
Fig. 352. Fusicladium artemisiae (WIS, lectotype of C. artemisiae). Conidiophores
and conidia. Scale bar = 10 µm. K. Bensch del.
artemisiae H.C. Greene, Amer. Midl. Naturalist 48(3): 757.
1952. Fig. 352.
Lectotype (designated here): USA, Wisconsin, Dane Co., Madison,
University of Wisconsin Arboretum, sandy slope, on living leaves of
Artemisia caudata (= A. campestris spp. caudata) (Asteraceae), 7
Jul. 1951, H.C. Greene (WIS). Isolectotype: BPI 426134. Topotype:
WIS, selected at the same locality at the 23 Jul. 1951.
≡ Fusicladium artemisiae (H.C. Greene) K. Schub. & U. Braun,
Mycotaxon 92: 57. 2005.
Ill.: Schubert (2005a: 58, ig. 2).
arthoniae M.S. Christ. & D. Hawksw., Bull. Brit. Mus. (Nat.
Hist.), Bot. 6(3): 210. 1979.
Holotype: Sweden, Skåne, Genarp, Häckeberga, on apothecia of
Arthonia impolita (Arthoniaceae) on Quercus (Fagaceae), 24 Apr.
1946, M. Skytte Christiansen (herb. Christiansen 570).
Lit.: Heuchert et al. (2005: 58).
Ill.: Hawksworth (1979: 211, ig. 10).
Notes: Excluded, not Cladosporium s. str. (rather Taeniolella-like).
arundinaceum P. Karst. – Oudemans (1924).
Notes: An error; Clasterosporium was intended.
Fig. 351. Fusicladium aromaticum (BPI 426124, lectotype of C. aromaticum).
Conidiophores arising from supericial hyphae and conidia. Scale bar = 10 µm. K.
Bensch del.
aromaticum Ellis & Everh., Proc. Acad. Nat. Sci. Philadelphia
47(3): 439. 1895. Fig. 351.
Lectotype (designated here): USA, California, Pasadena, on
living leaves of Rhus aromatica (Anacardiaceae), Aug. 1894, A.J.
McClatchie (BPI 426124). Isolectotype: NY.
≡ Fusicladium aromaticum (Ellis & Everh.) K. Schub. & U. Braun,
Fungal Diversity 20: 192. 2005.
= Cladosporium nervale Ellis & Dearn., in Bartholomew, Fungi Columb., Cent.
XXI, No. 2010. 1905.
Lit.: Saccardo (1899: 1079), Cash (1952: 69).
Ill.: Schubert & Braun (2005b: 193, ig. 2).
298
astericola Davis, Trans. Wisconsin Acad. Sci. 20: 428. 1922.
Fig. 353.
Lectotype (designated here): USA, Wisconsin, Mellen, on upper
leaves and upper portions of stems of Doellingeria umbellata (≡
Aster umbellatus) (Asteraceae), 4 Aug. 1919, J.J. Davis (WIS).
Isolectotype: BPI 426143.
≡ Fusicladium astericola (Davis) K. Schub. & U. Braun, Mycotaxon 92:
59. 2005.
Ill.: Schubert (2005a: 60, ig. 3).
asterinae Deighton, Mycol. Pap. 118: 30. 1969. Fig. 354.
Holotype: Sierra Leone, Kenema (Nongowa), on colonies of
Asterina contigua (Asterinaceae) on leaves of Dialium dinklagei
(Fabaceae), 6 Dec. 1937, F.C. Deighton (IMI 11851b).
≡ Parapericoniella asterinae (Deighton) U. Braun, Heuchert & K.
Schub., Schlechtendalia 13: 59. 2005.
Lit.: Ellis (1976: 331), Khan & Shamsi (1986: 111–112).
the genuS Cladosporium
Ill.: Deighton (1969: 31, ig. 17), Ellis (1976: 332, ig. 250 A), Khan &
Shamsi (1986: 112, ig. 1), Heuchert et al. (2005: 60, ig. 23).
asteroma Fuckel, Jahrb. Nassauischen Vereins Naturk. 23–
24: 355. “1869”, 1870.
Syntypes: Germany, between Hamburg and Wehrheim, on living
leaves of Populus tremula (Salicaceae), Fuckel, Fungi Rhen. Exs.
2208 (e.g., HAL).
Fig. 353. Fusicladium astericola (WIS, lectotype of C. astericola). Infected leaves
(original size), conidiophores and conidia. Scale bar = 10 µm. K. Bensch del.
≡ Napicladium asteroma (Fuckel) Allesch., Ber. Bayer. Bot. Ges. 5: 25.
1897.
≡ Napicladium asteroma (Fuckel) Sacc., Malphigia 17: 421. 1902.
= Oidium radiosum Lib., Pl. Crypt. Arduenna, Fasc. 3, 285. 1834. [Types: BR,
Libert, Pl. Crypt. Arduenna 285].
≡ Fusicladium radiosum (Lib.) Lind var. radiosum, Ann. Mycol. 3: 430.
1905.
≡ Fusicladium radiosum (Lib.) Lindau, Krypt.-Fl., ed. 2, 1(8): 777. 1907.
≡ Stigmina radiosa (Lib.) Goid., Ann. Bot. (Rome) 21: 11. 1936.
≡ Pollaccia radiosa (Lib.) E. Bald. & Cif., Atti Ist. Bot. “Giovanni Briosi”
10: 61. 1937.
≡ Venturia radiosa (Lib.) Ferd. & C. A. Jørg., Skovtraeernes Sygdomme
1: 125. 1938. (nom. anamorph.).
= Cladosporium ramulosum Roberge ex Desm., Ann. Sci. Nat. Bot., Sér. 2, 18:
361. 1852, non Reissek, 1851. [Type: PC].
≡ Fusicladium ramulosum Rostr., Tidsskr. Skovbr. 6: 294. 1883, nom.
nov., as “(Roberge, in Desm.) Rostr. ”.
≡ Pollaccia ramulosa (Rostr.) Ondřej, Eur. J. Forest Pathol. 2: 143. 1972,
nom. nov., as “(Desm.) Ondřej”.
= ? Cladosporium asteroma Fuckel var. macrosporum Sacc., Michelia 2(6):
126. 1882.
= Fusicladium tremulae A.B. Frank, Hedwigia 22: 127. 1883. [Type: B].
≡ Napicladium tremulae (A. B. Frank) Sacc., Syll. fung. 4: 482. 1886.
= ? Cladosporium asteroma Fuckel var. microsporum Sacc., Syll. fung. 4: 357.
1886.
≡ Fusicladium radiosum (Lib.) Lindau var. microsporum (Sacc.) Lindau, in
Rabenhorst, Krypt.-Fl., ed. 2, 1(8): 777. 1907.
= Venturia tremulae Aderh., Hedwigia 36: 81. 1897 var. tremulae.
= Fusariella populi Garb., Bull. Soc. Mycol. France 33: 89. 1917.
Lit.: Saccardo (1886: 357, 1913a: 1376), Lindau (1907: 777), Lind
(1913: 520), Baldacci & Ciferri (1937: 61), Sivanesan (1984: 618),
Schubert et al. (2003: 85).
asteroma var. macrosporum Sacc., Michelia 2(6): 126. 1880.
Type: France, on leaves of Populus alba (Salicaceae).
= ? Fusicladium radiosum (Lib.) Lind, Ann. Mycol. 3: 430. 1905.
Lit.: Schubert et al. (2003: 85).
asteroma var. microsporum Sacc., Syll. fung. 4: 357. 1886.
Type: Italy, Conegliano, on leaves of Populus tremula (Salicaceae).
= ? Fusicladium radiosum (Lib.) Lind, Ann. Mycol. 3: 430. 1905.
Lit.: Lindau (1907: 777), Baldacci & Ciferri (1937: 61), Sivanesan
(1984: 618), Schubert et al. (2003: 85).
aterrimum Ellis & Everh., Proc. Acad. Nat. Sci. Philadelphia
1894: 378. 1895.
Syntypes: USA, Kansas, Rockport, on rotten wood, Nov. 1893, E.
Bartholomew, No. 1256 (BPI 426152, NY 313201).
Fig. 354. Parapericoniella asterinae (IMI 11851b, holotype of C. asterinae).
Conidiophores with branched apices and lateral branchlets, detached ‘branchlets’
and conidia. Scale bar = 10 µm. B. Heuchert del.
www.studiesinmycology.org
= Helminthosporium binum Corda, Icon. fung. 6: 9. 1854, as “Helmisporium”.
[Type: PRM].
≡ Scolicotrichum binum (Corda) Sacc., Syll. fung. 4: 349. 1886.
≡ Spadicoides bina (Corda) S. Hughes, Canad. J. Bot. 36: 806. 1958,
as “binum”.
= Virgaria uniseptata Berk. & M.A. Curtis, Grevillea 3(28): 145. 1875.
[Syntypes: K, NYS].
≡ Cladotrichum uniseptatum (Berk. & M.A. Curtis) Sacc., Syll. fung. 4:
373. 1886.
≡ Scolicotrichum uniseptatum (Berk. & M.A. Curtis) Cooke, Grevillea
17(82): 41. 1888.
≡ Diplococcium uniseptatum (Berk. & M.A. Curtis) S. Hughes, Canad. J.
Bot. 31: 634. 1953.
= Cladotrichum simplex Sacc., Ann. Mycol. 4: 278. 1906. [Type: PAD].
299
BenSch et al.
= Cladotrichum tapesiae Sacc., Ann. Mycol. 6: 565. 1908.
Lit.: Saccardo (1895: 620), Cash (1952: 68), Hughes (1953: 634),
Ellis (1963: 8–9).
atrum Link, Ges. Naturf. Freunde Berlin Mag. Neuesten
Entdeck. Naturk. 7: 38. 1816 : Fr., Syst. mycol. 3(2): 371.
1832.
Type: Germany, Berlin, on dry stems of a herbaceous plant, Link.
= Dematium herbarum δ lignorum Alb. & Schwein., Consp. fung. lusat.: 368.
1805.
Lit.: Saccardo (1886: 362), Lindau (1907: 831), Hughes (1958:
Mycelia sterilia).
Notes: The material examined by Hughes (B 700006195) is a
sample on wood without any detailed information on the label. If
this material was part of Link’s herbarium is unclear. It contains
sterile mycelium as stated by Hughes (1958). All old collections
of C. atrum examined belonged to various wood-inhabiting
hyphomycetes, often to Torula herbarum (e.g. M-0057458).
aureum Link, Ges. Naturf. Freunde Berlin Mag. Neuesten
Entdeck. Gesammten Naturk. 7: 38. 1816.
Type: On rocks, sent by Nees von Esenbeck.
= ? Trentepohlia aurea (L.) Mart., Fl. crypt. erlang.: 351. 1817.
Notes: Nees (1817): “Cladosporium aureum Link ... bildet mit
Dematium petraeum und strigosum Pers. eine eigene, den Lichenen
beizuordnende Gattung, die ich Amphiconium nenne.”. The genus
Amphiconium Nees is synonymous with Trentepohlia Mart.
avellaneum G.A. de Vries, Contr. Knowl. Genus Cladosporium:
56. 1952 (f. avellaneum).
Ex-type culture: Netherlands, Utrecht, isol. from “Nivea” ointment,
19 May 1947 (ATCC 11273 = CBS 186.54 = IMI 49620).
≡ Cladosporium resinae f. avellaneum (G.A. de Vries) G.A. de Vries,
Antonie van Leeuwenhoek J. Microbiol. Serol. 21: 167. 1955.
= Amorphotheca resinae Parbery anamorph.
Lit.: David & Kelley (1995), Ho et al. (1999: 150), Partridge &
Morgan-Jones (2002: 344-348), Seifert et al. (2007).
Ill.: Partridge & Morgan-Jones (2002: 347, ig. 4), Seifert et al.
(2007: 236, ig. 1).
Notes: Seifert et al. (2007) examined and discussed this species, the
creosote fungus, in detail, including molecular data. Cladosporium
avellaneum is the oldest valid name for the anamorph of A. resinae,
but has often been confused with Hormodendrum resinae Lindau
(≡ Hormoconis resinae), the mononematous state of Sorocybe
resinae, the resin fungus.
avellaneum f. albidum G.A. de Vries, Contr. Knowl. Genus
Cladosporium: 56. 1952.
Notes: See C. avellaneum f. avellaneum. Monosporous isolate
from the parent culture (de Vries 1952).
avellaneum f. viride G.A. de Vries, Contr. Knowl. Genus
Cladosporium: 56. 1952.
Type: Netherlands, Utrecht, isol. from “Nivea” ointment, 19 May
1947. Ex-type culture: ATCC 11274 = CBS 187.54 = IMI 49621.
= Amorphotheca resinae Parbery anamorph.
Lit.: de Vries (1955: 167).
Notes: See C. avellaneum f. avellaneum. Monosporous isolate
from the parent culture (de Vries 1952).
bacilligerum Mont. & Fr., Ann. Sci. Nat. Bot., Sér. 2, 6: 31.
1836.
Type: France, Lyons, Rochecardon near “Lugdunum”, on Alnus
glutinosa (Betulaceae).
≡ Passalora bacilligera (Mont. & Fr.) Mont. & Fr., in Montagne, Syll. gen.
sp. crypt.: 305. 1856.
≡ Scolecotrichum bacilligerum (Mont. & Fr.) J. Schröt., in Cohn, Krypt.-Fl.
Schlesien, Bd. 3(2), Heft 4: 498. 1897.
Lit.: Cooke (1871: 584), Deighton (1967: 5–8), Crous & Braun
(2003: 440).
balladynae Deighton, Mycol. Pap. 118: 32. 1969. Fig. 355.
Holotype: Uganda, near Masaka, on Balladyna magniica
(Parodiopsidaceae) on leaves of Canthium vulgare (Rubiaceae),
May 1962, C.L.A. Leakey (IMI 98798i).
Lit.: Ellis (1976: 331), Heuchert et al. (2005: 61–62).
Ill.: Deighton (1969: 33, ig. 18), Ellis (1976: 332, ig. 250 B),
Heuchert et al. (2005: 62, ig. 24).
Notes: This species has to be excluded from Cladosporium s. str.
and seems to be congeneric with C. asterinae, which has been
assigned to the new genus Parapericoniella, but the type material
is too meagre for a inal conclusion (Heuchert et al. 2005).
bantianum (Sacc.) Borelli, Riv. Anat. Patol. Oncol. 17: 618.
1960.
Type: Italy, Florenz, isol. from cerebral granulomata, 1912 (PAD:
photomicrographs prepared by Saccardo).
Basionym: Torula bantiana Sacc., Ann. Mycol. 10: 320. 1912.
≡ Xylohypha bantiana (Sacc.) McGinnis, A.A. Padhye, Borelli & Ajello, J.
Clin. Microbiol. 23: 1150. 1986.
≡ Cladophialophora bantiana (Sacc.) de Hoog, Kwon-Chung &
McGinnis, J. Med. Veterin. Mycol. 33: 343. 1995.
= Cladosporium trichoides C.W. Emmons, Amer. J. Clin. Pathol. 22: 541.
1952.
= Cladosporium trichoides C.W. Emmons var. chlamydosporum Kwon-Chung,
Mycologia 75(2): 320. 1983.
Notes: See C. avellaneum f. avellaneum. Monosporous isolate
from the parent culture (de Vries 1952).
Lit.: Matsushima (1975), McGinnis & Borelli (1981), Kwon-Chung
& Bennett (1992: 639), Ho et al. (1999: 146), de Hoog et al. (2000:
564), Schell (2003: 577).
Notes: Xylohypha emmonsii, formerly reduced to synonymy with
Cladophialophora bantiana, was re-established as a separate
species despite high nDNA homology values, as Cladophialophora
emmonsii, in Gerrits van den Ende & de Hoog, Stud. Mycol. 43:
160, 1999.
avellaneum f. sterile G.A. de Vries, Contr. Knowl. Genus
Cladosporium: 56. 1952.
baptisiae H.C. Greene, Amer. Midl. Naturalist 39(2): 456.
1948. Fig. 356.
Ex-type culture: Netherlands, Utrecht, isol. from “Nivea” ointment,
19 May 1947 (CBS 185.54).
≡ Cladosporium resinae f. albidum (G.A. de Vries) G.A. de Vries, Antonie
van Leeuwenhoek J. Microbiol. Serol. 21: 167. 1955.
= Amorphotheca resinae Parbery anamorph.
Type: Netherlands, Utrecht, isol. from “Nivea” ointment, 19 May
1947.
≡ Cladosporium resinae f. sterile (G.A. de Vries) G.A. de Vries, Antonie
van Leeuwenhoek J. Microbiol. Serol. 21: 167. 1955.
= Amorphotheca resinae Parbery anamorph.
300
Lectotype (designated here): USA, Wisconsin, Dane Co., Madison,
University of Wisconsin Arboretum, on living leaves of Baptisia
leucophaea (= B. bracteata var. glabrescens) (Fabaceae), 14 Jul.
1947, H.C. Greene (WIS). Isolectotype: BPI 426163.
the genuS Cladosporium
Fig. 356. Fusicladium baptisiae (WIS, lectotype of C. baptisiae). Symptoms (original
size), conidia, conidiogenous cells and fascicles of conidiophores. Scale bar = 10
µm. K. Bensch del.
≡ Cercospora bellynckii (Westend.) Niessl, Hedwigia 15: 1. 1876.
≡ Cercospora bellynckii (Westend.) Sacc., Nuovo Giorn. Bot. Ital. 8: 818.
1876.
≡ Cercosporidium bellynckii (Westend.) X.J. Liu & Y.L. Guo, Acta Mycol.
Sin. 1(2): 93. 1982.
≡ Mycovellosiella bellynckii (Westend.) Constant., Cryptog. Mycol. 3(1):
67. 1982.
≡ Passalora bellynckii (Westend.) U. Braun, Mycotaxon 55: 228. 1995.
= Cercospora vincetoxici Sacc., Syll. fung. 15: 85. 1901.
Lit.: Saccardo (1886: 450), Lindau (1910: 129), Chupp (1954: 69),
Crous & Braun (2003: 78).
berkheyae Syd., Ann. Mycol. 12: 267. 1914.
Fig. 355. Cladosporium balladynae (IMI 98798i). Conidia and conidiophores. Scale
bar = 10 µm. B. Heuchert del.
≡ Fusicladium baptisiae (H.C. Greene) K. Schub. & U. Braun,
Mycotaxon 92: 62. 2005.
Ill.: Schubert (2005a: 62, Fig. 4).
beijerinckii Oudem., on www.indexfungorum.org, Kirk et al.
(n. d.).
Notes: An error, Coryneum beyerinckii Oudem., Hedwigia 22: 115.
1883 [= Stigmina carpophila (Lév.) M.B. Ellis, Mycol. Pap. 72: 56.
1959] was intended.
bellynckii Westend., Bull. Acad. Roy. Sci. Belgique 21(8):
240. 1854.
Holotype: Belgium, Bois de Dave, near Namur, on faded leaves
of Cynanchum vincetoxicum (= Vincetoxicum hirundinaria)
(Apocynaceae), Prof. Bellynck (BR).
www.studiesinmycology.org
Holotype: South Africa, Kwa-Zulu Natal, Cramond, on leaves of
Berkheya sp. (Asteraceae), 2 Dec. 1913, No. 6852 (S).
≡ Fulvia berkheyae (Syd.) M.B. Ellis, More Dematiaceous Hyphomycetes:
315. 1976.
≡ Mycovellosiella berkheyae (Syd.) U. Braun & Crous, Mycol. Res. 99(1):
32. 1995.
≡ Passalora berkheyae (Syd.) U. Braun & Crous, Mycosphaerella and
its anamorphs: 1. Names published in Cercospora and Passalora, CBS
Biodiversity Ser. 1: 441. 2003.
Lit.: Saccardo (1931: 789), Crous & Braun (1996: 250).
betuligenum Ellis & Galloway, in herb. Fig. 357.
Specimen: USA, Washington District of Columbia, on Betula nigra (Betulaceae), 2
Oct. 1887, B.T. Galloway (BPI 426165).
≡ Fusicladium betuligenum K. Schub. & U. Braun, Nova Hedwigia 84:
190. 2007.
Ill.: Schubert & Braun (2007: 192, ig. 2).
bisporum Matsush., Icones Microfungorum a Matsushima
Lectorum: 33. 1975.
Holotype: Japan, Iriomote Island, Okinawa, on a dead leaf of Musa
×paradisiaca (Musaceae), Mar. 1974 (Matsushima herb. 4861).
301
BenSch et al.
≡ Beejadwaya bispora (Matsush.) Subram., Kavaka 5: 97. 1977.
Ill.: Matsushima (1975: pl. 188, igs 1–2).
Notes: Subramanian (1977) transferred the species into a new
genus because of the characteristically shaped, 1-celled conidia
consistently formed in chains of two.
brachyelytri H.C. Greene, Trans. Wisconsin Acad. Sci. 53:
214. 1964. Fig. 358.
Lectotype (designated here): USA, Wisconsin, Sawyer
Co., Flambeau State Forest near Oxbow, on living leaves of
Brachyelytrum erectum (Poaceae), 22 Jul. 1964, H.C. Greene
(WIS). Isolectotype: BPI 426166.
= Passalora fusimaculans (G.F. Atk.) U. Braun & Crous var. barretoana U.
Braun & Crous, Mycosphaerella and its anamorphs: 1. Names published in
Cercospora and Passalora. CBS Biodiversity Ser. 1: 453. 2003.
≡ Passalora barretoana (U. Braun & Crous) D.J. Soares, U. Braun &
R.W. Barreto, Australas. Pl. Pathol. 35(3): 348. 2006.
Lit.: Schubert (2005a: 63–65).
Ill.: Schubert (2005a: 64, ig. 5).
brachytrichum Corda, Icon. fung. 1: 14. 1837.
Syntypes: Czech Republic, near Reichenberg, on the inner side of
the bark of Fagus sp. (Fagaceae), Corda (PRM 155419, 155420).
≡ Didymotrichum brachytrichum (Corda) Bonord., Handb. Mykol.: 89.
1851.
Fig. 357. Fusicladium betuligenum (BPI 426165, holotype material). Conidiophores,
conidiogenous cells and conidia. Scale bar = 10 µm. K. Bensch del.
Lit.: Saccardo (1886: 354), Lindau (1907: 821).
Ill.: Corda (1837: tab. 4, ig. 209).
Notes: Excluded, not Cladosporium s. str. This species is
Cladosporium-like, but the conidiogenous loci and conidial hila are
subdenticulate and not cladosporioid (coronate). The pale conidia
remind one of those of “Cladosporium elatum”.
brevipes Ellis & Barthol. → subsessile.
brevipes House, Bull. New York State Mus. Nat. Hist.
219/220: 62. 1919–1920.
Notes: This appears to be an error by Petrak. Although page 62
of vol 219/220 reads “CLADOSPORIUM BREVIPES, N. sp.” this
portion of vol. 219/220 is actually a reprint of Peck’s report of 1886
(1887), reproduced in vol. 219/220 for reasons stated on page 36.
Homer D. House authored in 1921 “Notes on Fungi, IV…from New
York State Museum Bulletins 219, 220” but House’s notes make no
reference to C. brevipes or any other Cladosporium. It seems that
Petrak’s reference to C. brevipes House is an error. Cladosporium
letiferum House was likely created via the same error. Both actually
pertain to Peck’s fungi of the same name.
brevipes Peck, Rep. (Annual) New York State Mus. Nat. Hist.
40: 64. 1887.
Holotype: USA, New York, Menands, on living leaves of Quercus
alba (Fagaceae), July, C.H. Peck (NYS 523).
Lit.: Saccardo (1892: 604).
Notes: Excluded, not Cladosporium s. str., but taxonomic status
unclear.
breviramosum Morgan-Jones, Mycotaxon 32(1): 228. 1988.
Fig. 358. Passalora barretoana (lectotype of C. brachyelytri). Infected leaves,
conidia, conidiogenous cells and small fascicles of conidiophores emerging through
stomata. Scale bar = 10 µm. K. Bensch del.
302
Holotype: USA, Georgia, St. Simon’s Island, King and Prince Hotel,
on discoloured wallpaper, Dec. 1987, B.J. Jacobsen (AUA). Extype culture: ATCC 64696 = ATCC 76215.
Lit.: Ho et al. (1999: 119).
Ill.: Morgan-Jones & Jacobsen (1988: 229, ig. 2; 231, pl. 1), Ho et
al. (1999: 117, igs 4–5).
the genuS Cladosporium
Fig. 359. Fusicladium britannicum (IMI 175936, holotype of C. britannicum).
Conidiophores, conidia, hyphae and swollen hyphal cells. Scale bar = 10 µm. U.
Braun del.
Notes: Not Cladosporium s. str., this species clusters together with
Amorphotheca resinae and not within the large Cladosporium clade
(Braun et al. 2003, Seifert et al. 2007).
britannicum M.B. Ellis, More Dematiaceous Hyphomycetes:
328. 1976. Fig. 359.
Holotype: UK, Wales, Pwee-y-Faeda Estate, on dead wood of
Quercus sp. (Fagaceae), 13 May 1973 (IMI 175936).
≡ Fusicladium britannicum (M.B. Ellis) U. Braun & K. Schub.,
Mycotaxon 103: 211. 2008.
Ill.: Ellis (1976: 327, ig. 245 C), Braun et al. (2008a: 212, ig. 2).
brunneoatrum McAlpine, Fungus Dis. Citrus Trees Austral.:
15, 78. 1899.
Holotype: Australia, New South Wales, Sydney, on fruits of Citrus
×aurantium (Rutaceae), Jul. 1898 (VPRI).
Lit.: Saccardo (1902: 1057).
Ill.: McAlpine (1899: pl. 1, igs 17–18).
Notes: Excluded, not Cladosporium s. str., but status still unclear;
type material very sparse.
butyri O. Jensen, Centralbl. Bakteriol., 2. Abth., 8: 311–312.
1902.
Type: On butter (location unknown).
= Monilia nigra Burri & W. Staub, Landw. Jahrb. Schweiz 23: 479. 1909.
Notes: A separate, yeast-like form and other characters do not
enable this species to be placed in Cladosporium (de Vries 1952:
90). Material from BPI (Transfer on C.M.A. and Malt, received from
Dr. Westerdijk, May 5, 1911, BPI 426174) has been examined. This
is probably from the culture that had also been examined by de
Vries. De Vries (1952) cited this species as published in “Landw.
Jahrb. Schweiz 15: 329. 1901”, but that reference did not contain a
description of this species. The whole paper of Burri & Staub (1909:
www.studiesinmycology.org
Fig. 360. Fusicladium caducum (WIS, lectotype of C. caducum). Symptoms (original
size), conidia, detached conidiogenous cells (ramoconidia) and conidiophores
arising from supericial hyphae. Scale bar = 10 µm. K. Bensch del.
479–513) dealt with Monilia nigra, but on page 479 the new name
appeared for the irst time.
caducum Davis, Trans. Wisconsin Acad. Sci. 21: 298. 1924.
Fig. 360.
Lectotype (designated here): USA, Wisconsin, along the
Wisconsin river, on leaves of Betula nigra (Betulaceae), 21 Jul.
1922, J.J. Davis (WIS). Isolectotype: BPI 426179.
≡ Fusicladium caducum (Davis) K. Schub. & U. Braun, Mycotaxon 92:
65. 2005.
Ill.: Schubert (2005a: 66, Fig. 6).
caesalpiniae Sawada, Rep. Gov. Res. Inst. Formosa 85: 91.
1943, nom. inval. Fig. 361.
Holotype: Taiwan, Kaohsiung Prov., on Caesalpinia crista (= C.
nuga) (Fabaceae), 24 Mar. 1930, K. Sawada (BPI 426182). Isotype:
PPMH.
≡ Fusicladium caesalpiniae K. Schub. & U. Braun, Fungal Diversity 20:
196. 2005.
Ill.: Schubert & Braun (2005b: 195, ig. 3).
caespitosa (Byssus) Roth, Catal. Bot. 1: 215. 1797.
Notes: This name was sometimes cited as possible synonym of
C. herbarum. This is, however, very doubtful and unproven. Roth
published a very brief description and cited Dillenius (Hist. Mus.:
4, tab. 1, ig. 7, 1741). His description as well as the illustration
in Dillenius, only showing some ilamentose structures, are not
interpretable. Furthermore, in Berlin (herb. B) no material is
deposited under the name Byssus caespitosa.
calamigenum Berk. & Broome, J. Linn. Soc., Bot. 14: 99.
1873, 1875, as “calamigena”.
Isotype: India, Ceylon [Sri Lanka], south of the island, on the fruit of
Calamus sp. (Arecaceae), Jul. 1868, G.H.K. Thwaites (K 121549).
303
BenSch et al.
Fig. 361. Fusicladium caesalpiniae (BPI 426182, holotype of C. caesalpiniae).
Conidiophores and conidia. Scale bar = 10 µm. K. Bensch del.
Lit.: Saccardo (1886: 367).
Notes: Excluded, probably galls.
callae Peck & Clinton, in herb. Fig. 362.
Specimen: USA, New York, Buffalo, on leaves of Calla sp. (Araceae), G.W. Clinton
(BPI 426184: syntype material of Cercospora callae Peck & Clinton).
≡ Cercospora callae Peck & Clinton, Rep. (Annual) New York State Mus.
Nat. Hist. 29: 52. 1876.
Lit.: Chupp (1954: 57), Schubert & Braun (2007: 190).
Ill.: Schubert & Braun (2007: 191, ig. 1).
calotropidis F. Stevens, Trans. Illinois State Acad. Sci. 10:
207. 1917.
Syntypes: Puerto Rico, on leaves of Calotropis procera
(Apocynaceae), Jul. 1915 (ILL 15842, IMI 19791, K, MICH, PC).
= Cercospora calotropidis Ellis & Everh., Rep. (Annual) Missouri Bot. Gard. 9:
120. 1898. [Types: BPI 433953, 433956, IMI 7752 (slide), NY].
≡ Phaeoramularia calotropidis (Ellis & Everh.) Kamal, A.S. Moses & R.
Chaudhary, Mycol. Res. 94: 716. 1990.
≡ Passalora calotropidis (Ellis & Everh.) U. Braun, Schlechtendalia 5:
60. 2000.
= Cercospora microsora Pat., in R.P. Duss, Champignons de la Guadeloupe,
3e Sér.: 91. 1902, nom. illeg., homonym, non C. microsora Sacc., 1880.
≡ Cercospora patouillardii Sacc., Syll. fung. 18: 608. 1906.
= Cercospora calotropidis Lingelsh., Bot. Jahrb. Syst. 39: 605. 1907, nom.
illeg., homonym, non C. calotropidis Ellis & Everh., 1898.
≡ Cercospora lingelsheimii Săvul. & Rayss, Ann. Cryptog. Exot. 8: 49.
1935.
= Cercospora inconspicua Pat. & Har., Bull. Soc. Mycol. France 24: 16. 1909.
[Type: FH 7807].
= Napicladium calotropidis H. Morstatt, Ann. Mycol. 10: 451. 1912.
= Cercospora calotropidis Speg., Anales Mus. Nac. Hist. Nat. Buenos Aires
26: 132. 1914, nom. illeg., homonym, non C. calotropidis Ellis & Everh., 1898.
≡ Cercosporina calotropidis Sacc., Syll. fung. 25: 897. 1931, as “(Speg.)
Sacc.”.
= Cercospora domingensis Gonz. Frag. & Cif., Bol. Soc. Esp. Hist. Nat. 26:
339. 1926. [Types: BPI 435826, MA].
Lit.: Saccardo (1931: 789, 869, 897), Subramanian (1971: 293),
Crous & Braun (2003: 96).
“cancerogenes von Niessen, Canceromyces Auct. 1894” (no
reference to an original description has been found).
Type: Isol. from a cancer of uterus.
304
Fig. 362. Cercospora callae (BPI 426184, syntype). Conidiophores and conidia.
Scale bar = 10 µm. K. Bensch del.
Lit.: Vuillemin (1931: 77), Nannizzi (1934).
Notes: “Wartmann credette di identiicarlo con il C. herbarum o con
una specie vicina, cioè con un saproita banale” (Nannizzi 1934).
capsici Kovatsch., Z. Planzenkrankh. Planzenschutz
48(7): 335. 1938, nom. nov., as “(É.J. Marchal & Steyaert)
Kovatsch.”.
Type: Democratic Republic of the Congo, Prov. di l’Equateur, on
Capsicum frutescens (Solanaceae).
≡ Cercospora capsici É.J. Marchal & Steyaert, Bull. Soc. Roy. Bot.
Belgique 61: 167. 1929, nom. illeg., homonym, non C. capsici Heald &
F.A. Wolf, 1911.
= Cercospora capsici Unamuno, Bol. Soc. Esp. Hist. Nat. 32: 161. 1932, nom.
illeg., homonym, non C. capsici Heald & F.A. Wolf, 1911.
≡ Cercospora unamunoi Castell., Rivista Agric. Subtrop. Trop. 42: 20.
1948.
≡ Phaeoramularia unamunoi (Castell.) Munt.-Cvetk., Lilloa 30: 183. 1960,
nom. inval.
= Cercospora capsicicola Vassiljevsky, Parazitnye nesovershennye griby,
Ch. I, Gifomitsety: 344. 1937.
≡ Phaeoramularia capsicicola (Vassiljevsky) Deighton, More
Dematiaceous Hyphomycetes: 323. 1976.
≡ Phaeoramularia capsicicola (Vassiljevsky) Deighton, Trans. Brit. Mycol.
Soc. 67: 140. 1976, comb. superl.
≡ Passalora capsicicola (Vassiljevsky) U. Braun & F. Freire, Cryptog.
Mycol. 23: 299. 2002.
Lit.: Chupp (1954: 553), Saccardo (1972: 1336), Crous & Braun
(2003: 103).
carpophilum Thüm., Oesterr. Bot. Z. 27: 12. 1877. Fig. 363.
Neotype: Austria, Klosterneuburg, on fruits of Prunus persica
(Rosaceae), Aug. 1877, Thümen (PAD).
≡ Fusicladium carpophilum (Thüm.) Oudem., Verh. Kon. Ned. Akad.
Wetensch., Afd. Natuurk., Tweede Sect. 1900: 388. 1900.
≡ Megacladosporium carpophilum (Thüm.) Vienn.-Bourg., Les
champignons parasite des plantes cultivées 1: 489. 1949.
≡ Fusicladosporium carpophilum (Thüm.) Partridge & Morgan-Jones,
Mycotaxon 85: 362. 2003.
= Fusicladium pruni Ducomet, Thèse Fac. Sci. Paris: 137. 1907.
= Fusicladium amygdali Ducomet, Ann. École, Natl. Agric. Rennes 4: 11. 1911.
= Cladosporium americanum H.C. Greene, Amer. Midl. Naturalist 41(3): 723.
1949.
= Venturia carpophila E.E. Fisher, Trans. Brit. Mycol. Soc. 44: 339. 1961.
the genuS Cladosporium
Lit.: Saccardo (1913a: 1371).
Ill.: Johan-Olsen (1897: tab. 4–5, igs 7–13).
Notes: Excluded. A yeast-like fungus, not allied to Cladosporium.
castellanii Borelli & Marcano, Castellania 1(5): 154. 1973.
Type: Venezuela, isol. from a patient with “tinea nigra” (IMI 183818).
= Stenella araguata Syd., Ann. Mycol. 28: 205. 1930.
Lit.: McGinnis & Padhye (1978: 415), Crous et al. (2007c: 43–45).
Ill.: Crous et al. (2007c: 44–45, igs 7–8).
catamarcense Speg., Anales Soc. Ci. Argent. 10: 63. 1880,
as “catamarcensis”.
Fig. 363. Fusicladium carpophilum (WIS, lectotype of C. americanum). Conidia and
conidiophores. Scale bar = 10 µm. K. Bensch del.
Lit.: Saccardo (1886: 353), Lind (1913), Ellis (1971: 317), Sivanesan
(1974: 75, 1984: 609), Schubert et al. (2003: 26, 28–30), Schubert
(2005a: 56–57).
Notes: “Herb. Mycol. Oecon. 599, Wien, 1877 (LE: syntype)” was
cited in error as type by Schubert et al. (2003).
carrionii Trejos, Revista Biol. Trop. 2: 106. 1954.
Type: Australia and Venezuela, isol. from a case of
chromoblastomycosis, (location unknown).
≡ Cladophialophora carrionii (Trejos) de Hoog, Kwon-Chung &
McGinnis, J. Med. Veterin. Mycol. 33: 345. 1995.
= Cladophialophora ajelloi Borelli, Pan-Amer. Health Organ. Sci. Publ. 396:
335. 1980.
Lit.: Kwon-Chung & Bennett (1992: 350), Ho et al. (1999: 147), de
Hoog et al. (2000: 570), Schell (2003: 578).
caryigenum (Ellis & Langl.) Gottwald, Mycologia 74(3): 388.
1982.
Lectotype (selected by Schubert et al., 2003): USA, Louisiana, St.
Martin, on leaves of Carya illinoensis (Juglandaceae), 3 Sep. 1888,
A.B. Langlois, Fl. Ludov. 1499 (NY). Isolectotypes: USA, Louisiana,
St. Martinsville, on leaves of Carya illinoensis (C. olivaeformis),
Sep. 1888, A.B. Langlois (BPI 426315, 426333, M).
≡ Fusicladium caryigenum Ellis & Langl., J. Mycol. 4: 124. 1888.
= Fusicladium effusum G. Winter, J. Mycol. 1: 101. 1885.
Lit.: Ho et al. (1999), Schubert & Braun (2002a).
caryigenum var. carpineum (Ellis & Everh.) Gottwald,
Mycologia 74(3): 389. 1982, as “carpinum”, comb. inval.
Lectotype (selected by Schubert et al. 2003): Canada, London, on
Carpinus americana (Betulaceae), Oct. 1889, J. Dearness (NY).
Isolectotypes: DAOM, M.
Basionym: Fusicladium effusum var. carpineum Ellis & Everh.,
Proc. Acad. Nat. Sci. Philadelphia 1891: 91. 1891.
Holotype: Argentina, “in arenosis alpinis de Catamarca”, on wilting
leaves of Pachylaena atriplicifolia (Asteraceae) (LPS 13.129).
Lit.: Saccardo (1886: 363), Farr (1973).
Notes: Excluded, but taxonomic status remains unclear. Type
material very sparse.
cattleyae
Verpl.,
Meded.
Landbouwhoogeschool
Opzoekingsstat. Staat Gent 3: 103. 1935.
Holotype: Belgium, Antwerpen, botanical garden, on dead leaves
of Cattleya mossia (Orchidaceae), 12 Mar. 1935 (GENT).
= Dendryphiella vinosa (Berk. & M.A. Curtis) Reisinger, Bull. Soc. Mycol.
France 84(1): 27. 1968.
Lit.: Schubert & Braun (2004: 313–314).
cellare (Pers.) Schanderl, Zentralbl. Bakteriol., 2. Abt., 94:
117. 1936.
Type: From a wine vault.
Basionym: Racodium cellare Pers., Neues Mag. Bot. 1: 123. 1794.
=
=
=
=
=
≡ Zasmidium cellare (Pers.) Fr., Summa veg. Scand. 2: 407. 1849.
≡ Rhinocladiella cellaris (Pers.) M.B. Ellis, Dematiaceous Hyphomycetes:
248. 1971.
Byssus septica Roth, Fl. Germ. 1: 516. 1788.
Byssus mollissima Ehrh., Pl. Crypt. Linn., No. 217. 1790.
Byssus cryptarum DC., Fl. franç. 2: 67. 1805.
Antennaria cellaris Fr., Syst. mycol. 3: 229. 1832.
Rhinocladiella ellisii Hawksw., Taxon 26(2–3): 208. 1977.
Lit.: Gonzáles-Fragoso (1927: 249), de Vries (1952), Barron (1968:
266), Arzanlou et al. (2007).
Notes: The well-known wine-cellar fungus does not belong in
Cladosporium s. str. Using molecular approaches, Arzanlou et al.
(2007) showed that this fungus clusters within the Mycosphaerellaceae
clade (Capnodiales). Morphologically it is close to Stenella. The
type species of Stenella (S. araguata) clusters, however, within
Teratosphaeriaceae (Capnodiales) (Crous et al. 2007c). Based on
phylogenetic as well as phenotypic characters, Zasmidium proved
to be the oldest genus name for Stenella-like anamorphs belonging
to Mycosphaerellaceae (Arzanlou et al. 2007). Although taxa with
a Zasmidium-like morphology appear to be paraphyletic within the
Mycosphaerellaceae (Crous et al. 2009a, b), these taxa should be
separated from Stenella, which has a different scar structure (pileate
conidiogenous loci versus planate, cercosporoid loci in Zasmidium)
and belongs to the Teratosphaeriaceae.
≡ Fusicladium carpineum (Ellis & Everh.) U. Braun & K. Schub., IMI
Descriptions of Fungi and Bacteria 152, No. 1512. 2002.
cerasi (Rabenh.) Aderh., Centralbl. Bakteriol., 2. Abth., 7:
656. 1901.
casei (Johan-Olsen) Sacc. & Traverso, Syll. fung. 19: 296.
1910.
Iconotype: Germany, Borussia, on fruits of Prunus cerasus
(Rosaceae) (Braun 1853: Tab. 1, B, 1–2).
Basionym: Acrosporium cerasi Rabenh., Verh. Vereins Beförd.
Gartenbaues Königl. Preuss. Staaten 1: 176. 1853.
Type: Norway, in cheese, (location unknown).
Basionym: Dematium casei Johan-Olsen, Centralbl. Bakteriol., 2.
Abth., 3: 280. 1897.
www.studiesinmycology.org
≡ Fusicladium cerasi (Rabenh.)
Lantbruksakad. Exp.-fält 1: 73. 1885.
Erikss.,
Meddeland.
Kongl.
305
BenSch et al.
≡ Fusicladium cerasi (Rabenh.) Sacc., Syll. fung. 4: 346. 1886. comb.
superl.
≡ Fusicladiopsis cerasi (Rabenh.) Karak. & Vassiljevsky, Parazitnye
nesovershennye griby, Ch. I. Gifomitsety: 210. 1937.
≡ Megacladosporium cerasi (Rabenh.) Vienn.-Bourg., Les champignons
parasites des plantes cultivées 1: 537. 1949.
≡ Karakulinia cerasi (Rabenh.) N.P. Golovina, Novosti Sist. Nizsh. Rast.
1: 213. 1964.
= Venturia cerasi Aderh., Landw. Jahrb. 29: 541. 1900.
Lit.: Sivanesan & Holliday (1981), Sivanesan (1984), Schubert et
al. (2003: 33–35).
cercestidis Deighton, Mycol. Res. 94(4): 570. 1990, non
Zasmidium cercestidis (J.M. Yen & Gilles) U. Braun, 2010.
Holotype: Sierra Leone, Njala (Kori), on living leaves of Cercestis
congensis (Araceae), 25 Apr. 1934, F.C. Deighton (IMI 7735).
≡ Stenella cercestidis (Deighton) U. Braun, Schlechtendalia 5: 54. 2000,
as “cercestis”, nom. illeg., non S. cercestidis (J.M. Yen & Gilles) Deighton,
1979.
≡ Stenella deightoniana U. Braun, Mycotaxon 92: 404. 2005.
≡ Zasmidium deightonianum (U. Braun) U. Braun, Schlechtendalia 20:
100. 2010.
cerophilum (Tubaki) Matsush., Icones Microfungorum a
Matsushima Lectorum: 34. 1975.
Type: Japan, on the blackened (originally white), powdery wax
layer under the leaf sheathes of Sasa sp. (Poaceae), May 1955
(preserved in Nagao Institute).
Basionym: Acrotheca cerophila Tubaki, J. Hattori Bot. Lab. 20: 143.
1958.
≡ Ramichloridium cerophilum (Tubaki) de Hoog, Stud. Mycol. 15: 74. 1977.
chaetomium Cooke, Grevillea 17(83): 66. 1889.
Fig. 364. Graphiopsis chlorocephalum (HAL 1924 F). Periconioid, stem rotting
morph. Conidiophores and conidia. Scale bar = 10 µm. K. Bensch del.
Type: USA, New Jersey, Newield, on leaves of Euphorbia sp.
(Euphorbiaceae), J.B. Ellis, No. 2289 (K).
≡ Cercosporidium chaetomium (Cooke) Deighton, Mycol. Pap. 112: 27.
1967.
≡ Passalora chaetomium (Cooke) Arx, Proc. Kon. Ned. Akad. Wetensch.
C, 86(1): 44. 1983.
≡ Passalora chaetomium (Cooke) Poonam Srivast., J. Liv. World 1(2):
114. 1994, comb. inval.
= Scolecotrichum ? euphorbiae Tracy & Earle, Bull. Torrey Bot. Club 23: 209.
1896. [Type: NY].
≡ Pyricularia euphorbiae (Tracy & Earle), G.F. Atk., Cornell Univ. Sci. Bull.
3(1): 40. 1897.
Lit.: Saccardo (1892: 602), Ellis (1971: 281), Crous & Braun (2003:
445).
chlamydeum Cif. & Redaelli, Mycopathol. Mycol. Appl. 8: 18.
1957.
Type: From skin of Canis.
Notes: “Material probably lost; judging from the description this was
Moniliella suaveolens” (de Hoog et al. 2000).
chlamydospora Matsush., Icones Microfungorum
Matsushima Lectorum: 34. 1975, as “chlamydosporis”.
a
Holotype: Japan, Osaka, Ibaraki City, from garden soil, May 1967
(Herb. Osaka 1047).
≡ Devriesia chlamydospora (Matsush.) Seifert & N.L. Nickerson,
Canad. J. Bot. 82: 922. 2004.
Fig. 365. Graphiopsis chlorocephalum (HAL 2011 F). Cladosporioid, leaf-spotting
morph. Conidiophores and conidia. Scale bar = 10 µm. K. Bensch del.
Ill.: Matsushima (1975: Pl. 55, ig. 3).
chlorocephalum (Fresen.) E.W. Mason & M.B. Ellis, Mycol.
Pap. 56: 123. 1953. Figs 364, 365.
Holotype: On dead stems of Paeonia sp. (Paeoniaceae) (not
preserved).
306
Neotype: Germany, Sachsen-Anhalt, Halle (Saale), Botanical
Garden, on dead stems of Paeonia oficinalis, 22 Jun. 2005, K.
Schubert (HAL 1924 F). Isoneotype: CBS H-19869. Ex-type
culture: CBS 121523.
the genuS Cladosporium
Basionym: Periconia chlorocephala Fresen., Beitr. Mykol. 1: 21.
1850.
≡ Haplographium chlorocephalum (Fresen.) Grove, Sci. Gossip 21: 198.
1885.
≡ Graphiopsis chlorocephala (Fresen.) Trail, Scott. Naturalist (Perth)
10: 75. 1889.
≡ Dichocladosporium chlorocephalum (Fresen.) K. Schub., U. Braun &
Crous, Stud. Mycol. 58: 96. 2007.
= Cladosporium paeoniae Pass., in Thümen, Herb. Mycol. Oecon., Fasc. IX,
No. 416. 1876 and Just’s Bot. Jahresber. 4: 235. 1876.
= Periconia ellipsospora Penz. & Sacc., Atti Reale Ist. Veneto Sci. Lett. Arti,
Ser. 6, 2: 596. 1883–1884.
= Cladosporium paeoniae Pass. var. paeoniae-anomalae Sacc., Syll. fung. 4:
362. 1886.
= Haplographium chlorocephalum (Fresen.) Grove var. ovalisporum Ferraris,
Flora Ital. Crypt., Pars I, Fungi, Fasc. 13: 875. 1914.
Lit.: Saccardo (1886: 362), Lindau (1907: 822), Ferraris (1912:
348), Lind (1913: 524), de Vries (1952: 94), Ellis (1971: 309),
Subramanian (1971: 296–297), Ellis & Ellis (1985: 395), McKemy
& Morgan-Jones (1991a), Ho et al. (1999: 120), Schubert et al.
(2007a), Braun et al. (2008a: 208–209).
Ill.: Fresenius (1850: tab. 4, igs 10–15), Mason & Ellis (1953:
124–125, igs 42–43), Ellis (1971: 310, ig. 214 B), McKemy &
Morgan-Jones (1991a: 137, ig. 1; 139, pl. 1; 141, ig. 2; 143, pl.
2), Ho et al. (1999: 122, ig. 7), Schubert et al. (2007a: 98–100,
igs 2–4).
chodatii (Nechitsch) Sacc. & D. Sacc., Syll. fung. 18: 577.
1906.
Type: On fermentated rice (Oryza, Poaceae).
Basionym: Dematium chodatii Nechitsch, Inst. Bot. Univ. Genève,
Ser. 6, 5: 22. 1904, as “chodati”.
≡ Candida chodatii (Nechitsch) Berkhout, Die schimmelgeschlachten
Monilia, Oidium, Oospora en Torula: 54. 1923.
= Pullularia pullulans (de Bary) Berkhout, Die schimmelgeschlachten Monilia,
Oidium, Oospora en Torula: 55. 1923.
Lit.: Bilgrami et al. (1991).
Ill.: Nechitsch (1904: 23–25, igs 4–6).
Notes: Type material could not be traced and is probably not
preserved. A collection from BPI (“Transfer on Malt. Received
from Dr. Westerdijk, May 5, 1911”, BPI 426369), on which the
combination Candida chodatii had probably been based, was
examined. Subramanian (1971) cited the species as possible
synonym of Aureobasidium oleae, now regarded as A. pullulans
var. pullulans. The close afinity to Aureobasidium can be
conirmed. Brummitt & Powell (1992) cited the name of this
author as “Nechitsche”, but in the original publication from 1904
his name is given as “Nechitsch”.
cinnamomeum → cinnamomi
cinnamomi (Racib.) Höhn., in Kabát & Bubák, Fungi Imperf.
Exs., Fasc. XIII, No. 643. 1910, as “cinnamomeum”.
Syntype: Indonesia, Java, Buitenzorg, Tjenkumeh, on
Cinnamomum sp. (Lauraceae), 1908, F. v. Höhnel, Kabát & Bubák,
Fungi Imperf. Exs. 643 (e.g., PC).
Basionym: Scolecotrichum cinnamomi Racib., Paras. Alg. Pilz.
Javas: 40. 1900, as “cinnamomeum”.
≡ Stenella cinnamomi (Racib.) U. Braun, Schlechtendalia 8: 37. 2002, as
“cinnamomea”.
≡ Zasmidium cinnamomi (Racib.) Kamal & U. Braun, Cercosporoid
fungi of India: 240. 2010.
= Stenella cinnamomi Hosag. & U. Braun, Indian Phytopathol. 48: 261. 1995.
circinalis Grüss, Wochenschr. Brauerei 48(7): 67. 1931.
Type: On the surface of wort (location unknown).
www.studiesinmycology.org
Notes: From Grüss (1931): “Die Sporen entstehen am Ende
der Hyphen in Kettenform oder durch Verschiebung in kleine
Häufchen.” This collapse of chains into heads does not sound like
Cladosporium.
citri Briosi & Farneti, Atti Ist. Bot. Univ. Pavia, Ser. 2, 10: 19.
1907, nom. illeg., homonym, non C. citri Massee, 1899.
Type: Italy, Sicily, on fruits of Citrus medica (= C. ×limon)
(Rutaceae).
≡ Cladosporium farnetianum Sacc., Syll. fung. 22: 1366. 1913.
≡ Kurosawaia citri Hara, List of Japanese Fungi, ed. 4: 172. 1954, nom.
nov., as “(Briosi & Farneti) Hara”.
Lit.: Ferraris (1912: 347).
Notes: It is not a Cladosporium, but maybe Sphaceloma fawcetii.
citri Penz. – an error. See Fawcett (1910 and 1936: 536).
cladrastidis Naumov, Bull. Soc. Mycol. France 30: 80. 1914.
Holotype: Russia, Far East, Yuzhno-Ussurijskij Kraj, on leaves of
Maackia amurensis (= Cladrastis amurensis) (Fabaceae), 24 Jul.
1912, No. 10, N. Naumov (PC).
= Cercospora cladrastidis Jacz., Hedwigia 39: 123. 1900. [Types: HBG, LE
40382].
≡ Pseudocercospora cladrastidis (Jacz.) J.K. Bai & M.Y. Cheng, Acta
Mycol. Sin. 11: 121. 1992.
Lit.: Saccardo (1931: 792), Crous & Braun (2003: 126).
clappieri – listed in Unesco (1955).
Notes: Uncertain species, type material could not be traced.
coelosporum Spreng., Syst. veg. 4(1): 553. 1827, nom.
superl.
Type: Germany, on stems of Gramineae (Poaceae), (location
unknown).
= Dematium articulatum Pers., Neues Mag. Bot. 1: 121. 1794.
= Helminthosporium carispermum Link, ?, as “Helmisporium”.
Notes: This is a superluous name since Dematium articulatum was
cited as synonym. The latter name was also cited as synonym of C.
fasciculare. A sample deposited in the herbarium of Fresenius as
C. coelosporum has been examined (on old pods, “Fl. Fr. no. 963”,
J. Becker, FR), and proved to be C. herbarum.
compactum Berk. & M.A. Curtis, Grevillea 3(27): 106. 1875.
Types: North America, on leaves of Arundinaria sp. (Poaceae),
No. 3767 (IMI 69771, K, STR).
≡ Cercosporidium compactum (Berk. & M.A. Curtis) Deighton, Mycol.
Pap. 112: 59. 1967.
≡ Passalora compacta (Berk. & M.A. Curtis) U. Braun & Crous,
Mycosphaerella and its anamorphs: 1. Names published in Cercospora
and Passalora, CBS Biodiversity Ser. 1: 133. 2003.
= Cercospora scolecotrichoides G.F. Atk., Cornell Univ. Sci. Bull. 3(1): 46.
1897. [Types: CUP, IMI 95405].
Lit.: Saccardo (1886: 364), Lindau (1907: 833).
confusum Matsush., Matsushima Mycol. Mem. 3: 4. 1983.
Type: Canada, Ontario, on bark of Acer saccharum (Sapindaceae).
Ill.: Matsushima (1983: 28–29, igs 145–147).
Notes: Matsushima (1983) depicts phialosporous form.
congestum Berk. & Broome, J. Linn. Soc., Bot. 14: 99. 1873,
1875.
Types: India, Ceylon, on leaves of Litsea (Lauraceae) (K 115280,
PC).
= Spiropes scopiformis (Berk.) M.B. Ellis, Mycol. Pap. 114: 30. 1968.
307
BenSch et al.
Lit.: Saccardo (1886: 359).
Notes: See C. scopiforme. The material from PC reads: in Litzoa,
Ceylon [Sri Lanka], Dothidea, Berkeley.
coralloides W. Yamam., Sci. Rep. Hyogo Univ. Agric., Ser.
Agric. 4(1): 5. 1959, nom. inval.
Type: Japan, isol. from Ficus carica (Moraceae) and Oryza sativa
(Poaceae).
Lit.: Ho et al. (1999: 125), Bensch et al. (2010).
Ill.: Yamamoto (1959: 6, igs 17–20), Ho et al. (1999: 124, igs
12–13).
Notes: This species was not validly published, because the author
did not designate a type. The “lectotype” chosen in Ho et al. (1999)
is also incorrect since it is not an element from the protologue of
the original description. Hence, a formal validation of this name is
necessary, which will be proposed in a separate paper based on
a re-examination of this fungus. Excluded, but generic afinity not
yet clear.
coreopsidis H.C. Greene, Trans. Wisconsin Acad. Sci. 45:
190. 1956. Fig. 366.
Lectotype (designated here): USA, Wisconsin, Dane Co.,
Madison, University of Wisconsin Arboretum, on living leaves of
Coreopsis palmata (Asteraceae), 27 Jun. 1955 (WIS). Isolectotype:
BPI 426392.
≡ Fusicladium coreopsidis (H.C. Greene) K. Schub. & U. Braun,
Mycotaxon 92: 67. 2005.
Ill.: Schubert (2005a: 68, ig. 7).
cornigenum Bubák, Ann. K.K. Naturhist. Hofmus. 23: 106.
1909.
Holotype: Turkey, Stephanos, near Trapezunt, on living leaves of
Cornus australis (= C. sanguinea ssp. australis, Swida australis)
(Cornaceae), 7 Jul. 1907, Handel-Mazzetti, No. 214 (BPI 426393).
Lit.: Saccardo (1913a: 1367).
Notes: Excluded, conidiogenous cells probably monophialidic.
cubense R.F. Castañeda, Fungi Cubensis II: 4. 1987.
Fig. 366. Fusicladium coreopsidis (WIS, lectotype of C. coreopsidis). Conidia and
conidiophores. Scale bar = 10 µm. K. Bensch del.
cyclaminis Massey & Tilford, Phytopathology 22(1): 19. 1932.
Lectotype: USA, on Cyclamen sp. (Primulaceae), “received Aug.
1932 from L.M. Massey” (BPI 426434, part of type culture).
= Ramularia cyclaminicola Trel., Trans. Illinois State Acad. Sci. 9: 145. 1916.
[Type: ILL 14246: holotype].
Lit.: Baker et al. (1950), Braun (1998: 226), Zhang et al. (1999b:
38).
Notes: This species, previously only known from North America,
has recently been reported from Asia (China) by Zhang et al.
(1999b).
cyttariicola Speg., Physis (Buenos Aires) 7(23): 20. 1923, as
“cyttariicolum”.
Holotype: Argentina, Tierra del Fuego, Puerto Garibaldi, on
Cyttaria harioti (Cyttariaceae, Ascomycetes) (LPS 13.078).
Lit.: Farr (1973: 251, as “cyttariicolum”), Heuchert et al. (2005: 63).
Notes: Excluded, no Cladosporium s. str., but status unclear.
Holotype: Cuba, prov. Guantánamo, Maisí, on fallen leaves of Ficus
sp. (Moraceae), 24 Apr. 1986, Mayra Camino (INIFAT C86/134).
Isotype: HAL 2019 F.
daphniphylli Sawada, Rep. Gov. Res. Inst. Formosa 85: 91.
1943, nom. inval.
Ill.: Castañeda (1987: ig. 8), Crous et al. (2007b: 21, ig. 10).
Holotype:
Taiwan,
on
Daphniphyllum
glaucescens
(Daphniphyllaceae), 8 Feb. 1931, K. Sawada (PPMH).
Notes: Diagnosis only in Japanese, not validly published. Excluded,
but generic afinity not yet clear.
≡ Penidiella cubensis (R.F. Castañeda) U. Braun, Crous & R.F.
Castañeda, Stud. Mycol. 58: 19. 2007.
cubisporum Berk. & M.A. Curtis, Grevillea 3(27): 107. 1875.
Type: USA, Maine, on Ribes sp. (Grossulariaceae), Rev. J. Blake,
No. 6318 (K).
≡ Coremiella cubispora (Berk. & M.A. Curtis) M.B. Ellis, Dematiaceous
Hyphomycetes: 33. 1971.
≡ Briosia cubispora (Berk. & M.A. Curtis) Arx, Antonie van Leeuwenhoek
J. Microbiol. Serol. 38(3): 293. 1972.
Lit.: Saccardo (1886: 355).
cumulus Preuss, Linnaea 25: 726. 1851, as “cumulum”.
Holotype: Germany, Hoyerswerda, on fallen branches, C.G.T.
Preuss 382 (B 700006229).
Lit.: Saccardo (1886: 356), Lindau (1907: 810).
Notes: Excluded. This is a dictyosporous, Stempylium-like
hyphomycete.
decolorans McAlpine, in herb.
Specimen: Australia, on Cynosurus cristatus (Poaceae) (VPRI).
dematiosum Ellis & Langl., in herb.
Specimen: USA, Louisiana, St. Martin, on rotten stems of oak (Quercus sp.,
Fagaceae), 23 Mar. 1888, A.B. Langlois, Fl. Ludoviciana 1264 (NY).
Notes: Excluded, not Cladosporium. Status unclear. Specimen in
Myc. Coll. (Cash 1952: 68).
dendriticum Desm. – Gola (1930).
dendriticum Wallr., Fl. crypt. Germ. 2: 169. 1833.
Syntypes: Germany, Thuringia, on leaves of Pyrus sylvestris
(= Malus malus) (Rosaceae) (B, STR).
≡ Fusicladium dendriticum (Wallr.) Fuckel, Jahrb. Nassauischen Vereins
308
the genuS Cladosporium
Naturk. 23–24: 357. “1869”, 1870.
≡ Passalora dendritica (Wallr.) Sacc., Mycoth. Ven., Cent. XII, No. 1246.
1876. [Michelia 1(2): 265. 1878].
= Venturia inaequalis (Cooke) G. Winter, Hedwigia 36: 81. 1897.
= Fusicladium pomi (Fr.) Lind, Dan. fung.: 521. 1913.
Lit.: Cooke (1871: 583), Saccardo (1886: 345), Lindau (1907: 779),
Sivanesan (1984: 616, as Spilocaea pomi), Ritschel (2001), Crous
& Braun (2003: 485), Schubert et al. (2003: 76).
Notes: Further synonyms are given in Schubert et al. (2003).
≡ Passalora effusa (Berk. & M.A. Curtis) U. Braun, Mycotaxon 55: 231.
1995.
= Cercospora polygonorum Cooke, Hedwigia 17: 39. 1878. [Type: K].
≡ Pseudocercospora polygonorum (Cooke) Y.L. Guo & X.J. Liu,
Mycosystema 4: 110. 1991.
= Helminthosporium hydropiperis Thüm., Rev. Mycol. (Toulouse) 1: 60. 1879.
[Types: Thümen, Mycoth. Univ. 1087, e.g., HAL].
≡ Cercospora hydropiperis (Thüm.) Speg., Bol. Acad. Nac. Ci. 9: 191.
1880.
dendriticum Wallr. var. ß orbiculatum Berk., Gard. Chron.
1848: 716. 1848 (?).
Lit.: Saccardo (1886: 362, 447), Chupp (1954: 451, as Cercospora
polygonorum), Deighton (1986: 637), Crous & Braun (2003: 170).
Notes: In the original diagnosis three collections on different hosts
were mentioned, Deighton (1986) designated the collection on
Polygonum as lectotype. The specimens on Lobelia species refer
to a similar, but distinct Passalora.
dendriticum Wallr. var. heteromeles Harkn., 1881, in herb.
effusum (G. Winter) Demaree, J. Agric. Res. 37: 186. 1928,
nom. illeg., homonym, non C. effusum Berk. & M.A. Curtis,
1875.
Lit.: Cooke (1871: 583).
Notes: “Gard. Chron. 1848: 716 (1848)” has been checked, but the
name “var. orbiculatum” is not present.
Specimen: USA, California, on Heteromeles arbutifolia (Rosaceae), Jun. 1881 (BPI
426448).
= Fusicladium pomi (Fr.) Lind, Dan. fung.: 521. 1913.
Lit.: Ritschel (2001), Schubert et al. (2003: 76).
dendryphioides Ellis, in herb.
Specimen: USA, New Jersey, on Phytolacca sp. (Phytolaccaceae).
Notes: Specimen in Myc. Coll. (Cash 1952).
depressum Berk. & Broome, Ann. Mag. Nat. Hist., Ser. 2, 7:
99. 1851.
Type: UK, on Angelica sylvestris (Apiaceae) (K).
≡ Passalora depressa (Berk. & Broome) Sacc., Nuovo Giorn. Bot. Ital.
8: 187. 1876.
≡ Fusicladium depressum (Berk. & Broome) Roum., Fungi Sel. Gall. Exs.,
No. 86. 1879.
≡ Scolecotrichum depressum (Berk. & Broome) J. Schröt., Krypt.-Fl.
Schlesien, Bd. 3(2), Heft 4: 497. 1897.
≡ Cercospora depressa (Berk. & Broome) Vassiljevsky, Parazitnye
nesovershennye griby, Ch. I, Gifomicety: 385. 1937.
≡ Megacladosporium depressum (Berk. & Broome) Vienn.-Bourg., Les
champignons parasites des plantes cultivées 2: 1488. 1949, comb. inval.
≡ Cercosporidium depressum (Berk. & Broome) Deighton, Mycol. Pap.
112: 37. 1967.
≡ Passalora depressa (Berk. & Broome) Poonam Srivast., J. Liv. World
1(2): 114. 1994, comb. inval.
= Passalora polythrincioides Fuckel, Jahrb. Nassauischen Vereins Naturk.
23–24: 353. “1869”, 1870. [Type: Fuckel, Fungi Rhen. Exs. 103, e.g., HAL].
= Fusicladium peucedani Syd. & P. Syd., Ann. Mycol. 5: 340. 1907, nom. illeg.,
homonym, non F. peucedani Ellis & Holw., 1895. [Type: B, S].
= ? Mycosphaerella angelicae Woron., Věstn. Tilissk. Bot. Sada 28: 17. 1913.
= ? Cercospora depressa f. angelicae Dzhanuz., Trudy Vsesoyuzn. Inst.
Zashch. Rast. 19: 9. “1963”, 1964.
Lit.: Cooke (1871: 584), Lindau (1907: 786), Oudemans (1923),
Crous & Braun (2003: 157).
devriesii A.A. Padhye & Ajello, Sabouraudia 22(5): 430. 1984.
Type: Cayman Islands, from breast of Homo sapiens. Ex-type
culture: ATCC 56280 = CBS 147.84.
≡ Cladophialophora devriesii (A.A. Padhye & Ajello) de Hoog, KwonChung & McGinnis, J. Med. Veterin. Mycol. 33: 344. 1995.
Lit.: Kwon-Chung & Bennett (1992: 645), Ho et al. (1999: 147), de
Hoog et al. (2000: 573), Schell (2003: 578).
effusum Berk. & M.A. Curtis, Grevillea 3(27): 106. 1875.
Lectotype: USA, South Carolina, Society Hill, on Persicaria
punctata (≡ Polygonum punctatum) (Polygonaceae), No. 3775 (K).
Isolectotype: IMI 104922.
≡ Cercospora effusa (Berk. & M.A. Curtis) Ellis, J. Mycol. 1: 53. 1885.
≡ Didymaria effusa (Berk. & M.A. Curtis) Solheim, Illinois Biol. Monogr.
12: 65. 1930.
www.studiesinmycology.org
Holotype: USA, Illinois, Cobden Zels., on Carya tomentosa
(= Carya alba) (Juglandaceae), 1 Oct. 1882, F.S. Earle (B).
≡ Fusicladium effusum G. Winter, J. Mycol. 1: 101. 1885.
≡ Fusicladosporium effusum (G. Winter) Partridge & Morgan-Jones,
Mycotaxon 85: 364. 2003.
= Fusicladium caryigenum Ellis & Langl., J. Mycol. 4: 124. 1888. [Types: BPI,
M, NY].
≡ Cladosporium caryigenum (Ellis & Langl.) Gottwald, Mycologia 74(3):
388. 1982.
Lit.: Schubert & Braun (2002a), Schubert et al. (2003: 41–43).
elaeagnus Gapon., Uzbekistan Biol. Zurn. ?, 1964.
Type: Uzbekistan, Bucharskaya Oblast, on Elaeagnus
(Elaeagnaceae).
Lit.: Sagdullaeva et al. (1990: 47).
Ill.: Sagdullaeva et al. (1990: 48).
Notes: This name and incomplete reference was cited in
Sagdullaeva et al. (1990: 47). The name is not listed in Index
fungorum and MycoBank. V.A. Mel’nik checked the volumes of this
journal for several years before and after 1964, but failed to ind this
name, which has undoubtedly never been published.
elatum (Harz) Nannf., Svenska Skogsvardsfoereren Tidskr.
32(3–4): 397. 1934.
Type: Germany, on an old stump (location unknown).
Basionym: Hormodendrum elatum Harz, Bull. Soc. Imp. Naturalistes
Moscou 44(1): 140. 1871.
≡ Cadophora elatum (Harz) Nannf., Svenska Skogsvardsfoereren Tidskr.
32(3–4): 422. 1934.
≡ Ochrocladosporium elatum (Harz) Crous & U. Braun, Stud. Mycol.
58: 46. 2007.
Lit.: de Vries (1952: 67), Ellis (1976: 326), Matsushima (1983: 4),
Ho et al. (1999: 127), de Hoog et al. (2000: 585).
Ill.: de Vries (1952: 67, ig. 14), Minoura (1966: 141, ig. 4A), Ellis
(1976: 327, ig. 245 B), Matsushima (1983: 26–27, igs 143–144),
Ho et al. (1999: 126, ig. 17), de Hoog et al. (2000: 585–586, igs),
Crous et al. (2007c: 48, ig. 10).
elegans Matsush., Icones Microfungorum a Matsushima
Lectorum: 35. 1975, nom. illeg., homonym, non C. elegans
Penzig, 1882.
Type: Japan, Yaku Island, Kagoshima, isol. from garden soil, Jul.
1971 (Matsush. herb. 4109).
Ill.: Matsushima (1975: pl. 43).
Notes: Excluded, no true Cladosporium, but generic afinity not
yet clear. Zhang et al. (2003) reduced C. elegans Matsush. to
309
BenSch et al.
synonymy with C. elegans Penz., but the latter species proved to
be a synonym of C. herbarum and is therefore quite distinct.
elegans var. singaporense Sacc., Bull. Orto. Bot. Regia Univ.
Napoli 6: 60. 1921.
Holotype: Singapore, Botanical Garden, on leaves of Citrus acida
(Rutaceae), Aug. 1917, Baker, No. 4985 (PAD).
= Helminthosporium guareicola F. Stevens, Bot. Gaz. 65: 241. 1918.
≡ Spiropes guareicola (F. Stevens) Cif., Sydowia 9: 303. 1955.
≡ Pleurophragmium guareicola (F. Stevens) S. Hughes, Canad. J. Bot.
36: 797. 1958.
Lit.: Saccardo (1931: 795), Schubert & Braun (2005a: 107).
elsinoës H.C. Greene, Trans. Wisconsin Acad. Sci. 47: 127.
1958.
Lectotype (selected by Heuchert et al. 2005): USA, Wisconsin,
Lafayette Co., Ipswich near Platteville, on fructiications of
Elsinoë wisconsinensis (Elsinoaceae) on Desmodium illinoense
(Fabaceae), 16 Aug. 1951, H.C. Greene (WIS). Isotypes: BPI
426465A, BPI 426465B.
= Dendryphiella infuscans (Thüm.) M.B. Ellis, Dematiaceous Hyphomycetes:
500. 1971.
Lit.: Heuchert et al. (2005: 63–64).
Ill.: Heuchert et al. (2005: 64, ig. 25).
entoxylinum Corda, Icon. fung. 1: 14. 1837.
Holotype: Czech Republic, Reichenberg, on wood of Pinus sp.
(Pinaceae), Corda (PRM 155726).
Lit.: Saccardo (1886: 353), Lindau (1907: 811, 1910: 796),
Oudemans (1919, 1923).
Ill.: Corda (1837: tab. 3, ig. 202).
Notes: Excluded, no Cladosporium s. str. The conidiogenous
cells are unilocal, determinate or occasionally percurrent, the
conidiogenous loci and conidial hila are unthickened and noncoronate. This species is rather Taeniolella/Xylohypha-like. Several
collections under “Cladosporium entoxylinum” proved to be C.
herbarum s. lat. (e.g., on wood of Abies excelsa, Bohemia, Krypt.
Exs. 2837, PRM 481966; on wood of Carpinus betulus, Austria,
Wiener Wald, May 1936, K. Keissler, PRM 657474). The fungus
described and illustrated from China by Zhang et al. (2003) as
“Cladosporium entoxylinum” is quite distinct and the name is
misapplied. Oudemans (1923) listed C. entoxylinum as pathogen
of Sambucus racemosa (Caprifoliaceae).
erianthi Thüm., Rev. Mycol. (Toulouse) 1: 59. 1879.
Syntypes: USA, South Carolina, Aiken, on dry culms and leaves
of Saccharum giganteum (= Erianthus saccharoides) (Poaceae),
H.W. Ravenel, Thümen, Mycoth. Univ. 1766 (e.g., B, BPI 426532,
BR-MYC 81373,87, HAL, M, NY). Syntype or topotype material:
Roumeguère, Fungi Sel. Gall. Exs. 4690 (e.g., NY), issued in 1888.
Lit.: Saccardo (1886: 364).
Notes: Excluded, not Cladosporium s. str. This is a fungus with
Colletotrichum-like setae, large stromata and small, ovoidsubglobose to fusiform, pale brown conidia. Status unclear.
eriolobi Thaung, Trans. Brit. Mycol. Soc. 63(3): 620. 1974.
Fig. 367.
Holotype: Myanmar (Burma), Maymyo, Botanical Garden, on
living leaves of Eriolobus “indica” (Rosaceae), 28 Dec. 1972, Mya
Thaung (IMI 175732).
≡ Stenella eriolobi (Thaung) K. Schub. & U. Braun, Nova Hedwigia 84:
203. 2007.
≡ Zasmidium eriolobi (Thaung) K. Schub. & U. Braun, Schlechtendalia
20: 101. 2010.
Ill.: Thaung (1974: 621, ig. 2), Schubert & Braun (2007: 204, ig. 8).
eschscholtziae (Harkn.) Dingley, nom. ined.
Holotype: USA, California, San Francisco, on leaves of
Eschscholtzia californica (Papaveraceae), Jan. [1884], Harkness,
No. 3116 (destroyed).
Basionym: Heterosporium eschscholtziae Harkn., Bull. Calif. Acad.
Sci. 1: 38. 1884.
≡ Acroconidiella eschscholtziae (Harkn.)
Dematiaceous Hyphomycetes: 407. 1976.
M.B.
Ellis,
More
Lit.: David (1997: 111).
eucalypticola M.B. Ellis, on www.indexfungorum.org, in
herb.?, Kirk et al. (n. d.).
epacridis McAlpine, Victoria Naturalist 17(10): 186. 1901.
Holotype: Australia, Victoria, Caulield, on living leaves of Epacris
impressa (Ericaceae), Aug. 1900, C. French (VPRI).
Lit.: Saccardo (1902: 1058).
Notes: Excluded, not Cladosporium s. str., but taxonomic status
not yet clear.
episphaerium Schwein., Trans. Amer. Philos. Soc., N.S.,
4(2): 278. 1832, as “episphaeria”.
Syntypes: USA, Pennsylvania, Bethlehem, on Sphaeria species,
No. 2607 (PH 01020420, 01020421).
= ? Dematium episphaerium Alb. & Schwein., Consp. fung. lusat.: 369. 1805.
Lit.: Saccardo (1886: 369), Oudemans (1919, 1920: on Hypoxylon
rubiginosum, 1924).
Notes: Status unclear, no Cladosporium found in the type material.
epixilinum Corda – Gola (1930: 20).
Notes: See C. herbarum Link var. epixylinum Corda.
310
Fig. 367. Zasmidium eriolobi (IMI 175732, holotype of C. eriolobi). Conidiophores
and conidia. Scale bar = 10 µm. K. Bensch del.
the genuS Cladosporium
extoma Sacc. – Gola (1930: 20) listed the type at PAD.
Notes: Maybe this is an error in Gola (1930) and C. extorre was
intended.
extorre Sacc., Nuovo Giorn. Bot. Ital., N.S., 27: 86. 1920.
Holotype: USA, Wyoming, Tonington, on the bark of young twigs
of Whitney Crab Apple (= Pyrus coronaria) (Rosaceae), 12 Jun.
1917, V. Simmons, No. 10335 (PAD). Isotype: BPI 426542.
Lit.: Saccardo (1931: 795).
Notes: This species has to be excluded from Cladosporium s. str.;
status unclear.
fasciculare f. asparagi-oficinalis Thüm., Mycoth. Univ., Fasc.
1, No. 2066. 1881, nom. nud.
Type: USA, Carolina australis, on Asparagus oficinalis
(Asparagaceae), Aiken, 1877, H.W. Ravenel, Thümen, Mycoth.
Univ. 2066 (e.g. M-0057602).
Notes: In the original material from M, only an alternarioid
hyphomycete has been found.
fermentans Goto, Yamak. & Yokots., J. Agric. Chem. Soc.
Japan 49(7): 380. 1975.
Type: Japan, Kofu, from olive fruit waste, 12 Jun. 1968, S. Goto
(RIFY 0587).
= Pichia burtonii Boidin, Pignal, Lehodey, Vey & Abadie, Bull. Soc. Mycol.
France 80: 437. “1964”, 1965.
≡ Endomycopsis burtonii (Boidin, Pignal, Lehodey, Vey & Abadie) Kreger,
The Yeasts, Ed. 2: 174. 1970, nom. inval.
≡ Hyphopichia burtonii (Boidin, Pignal, Lehodey, Vey & Abadie) Arx &
Van der Walt, Antonie van Leeuwenhoek J. Microbiol. Serol. 42(3): 310.
1976.
Fig. 368. Passalora sweetiae (M-0057571, lectotype of C. ferrugineum).
Conidiophores and conidia. Scale bar = 10 µm. K. Bensch del.
ferrugineum Allesch., Hedwigia 34: 116. 1895. Fig. 368.
Lectotype (selected in Schubert & Braun 2005a): Brazil, Minas
Geraës, Paranahyba, on leaves of Sweetia bijuga (≡ Acosmium
bijugum) (Fabaceae), Jul. 1892, E. Ule, No. 1905 (M-0057571).
Isolectotypes: B 700006381, HBG, PC.
≡ Passalora sweetiae K. Schub. & U. Braun, Mycol. Progr. 4(2): 105.
2005.
Lit.: Saccardo (1895: 619).
Ill.: Schubert & Braun (2005a: 106, ig. 5).
ferrugineum R.F. Castañeda, Fungi Cubensis II: 4. 1987,
nom. illeg., homonym, non C. ferrugineum Allescher, 1895.
Holotype: Cuba, prov. Matanzas, Calimete, on living leaves of
Nectandra coriacea (Lauraceae), 24 Jan. 1987, R.F. Castañeda
(INIFAT C87/45). Ex-type culture: CBS 734.87, HAL 2018 F (dried
culture).
≡ Penidiella nectandrae Crous, U. Braun & R.F. Castañeda, Stud.
Mycol. 58: 20. 2007.
Ill.: Castañeda (1987: ig. 7), Crous et al. (2007b: 21, ig. 11).
ferrugineum Sacc. – Gola (1930: 20) listed the type at PAD.
Notes: Maybe an error in Gola (1930) and C. fumagineum was
intended.
lueggeae Thüm., ad. int., in Rabenhorst, Fungi Eur. Exs.,
Cent. XVI, No. 1571. 1872, nom. nud.
Syntypes: Greece, Athina, on leaves of Ophiopogon japonicus (≡
Flueggea japonica) (Asparagaceae), 20 Sep. 1869, de Heldreich,
Rabenhorst, Fungi Eur. Exs. 1571 (e.g., HAL, HBG).
Notes: Excluded, mixed infection with Alternaria sp., Colletotrichum
sp. and an unknown phialidic hyphomycete.
www.studiesinmycology.org
Fig. 369. Passalora foveolicola (LPS 13.136, holotype of C. foveolicola).
Conidiophores and conidia. Scale bar = 10 µm. K. Bensch del.
foveolicola Speg., Anales Mus. Nac. Buenos Aires 20: 437.
1910. Fig. 369.
Holotype: Argentina, near San Javier, Misiones, on living leaves
of Campovassouria cruciata (= Eupatorium bupleurifolium)
(Asteraceae), Aug. 1909 (LPS 13.136).
311
BenSch et al.
= Passalora foveolicola (Speg.) K. Schub. & U. Braun, Nova Hedwigia 84:
195. 2007.
Lit.: Saccardo (1913a: 1369), Farr (1973).
Ill.: Schubert & Braun (2007: 196, ig. 4).
fuligineum f. racemosum Bubák, in herb., as “racemosa”.
Specimen: Czech Republic, Bohemia, Kokocko, near Plzen, on a fungal fruit body
(holothecium), 10 Oct. 1910, F. Bubák (BPI 426582).
Notes: Not Cladosporium, but identity unclear.
fulvum (Arx) K. Bhalla & A.K. Sarbhoy → fulvum Cooke.
fulvum Cooke, Grevillea 12(61): 32. 1883.
Syntype: USA, South Carolina, on leaves of tomato (= Solanum
lycopersicum) (Solanaceae), Ravenel, Fungi Amer. Exs. 599 (e.g., K).
≡ Fulvia fulva (Cooke) Cif., Atti Ist. Bot. Lab. Crittog. Univ. Pavia 10(1):
245. 1954.
≡ Mycovellosiella fulva (Cooke) Arx, Proc. Kon. Ned. Akad. Wetensch.
C, 86(1): 48. 1983.
≡ Cladosporium fulvum “(Arx)” K. Bhalla & A.K. Sarbhoy, Indian
Phytopathol. 53(3): 262. 2000, nom. illeg.
≡ Passalora fulva (Cooke) U. Braun & Crous, Mycosphaerella and its
anamorphs: 1. Names published in Cercospora and Passalora, CBS
Biodiversity Ser. 1: 453. 2003.
Lit.: Saccardo (1886: 363), Lindau (1907: 829, 1910: 797), Ferraris
(1912: 349, 1914: 885), de Vries (1952: 70), Ellis (1971: 306–307),
Holiday & Mulder (1976), von Arx (1987: 195), Ho et al. (1999: 128).
fulvum Cooke var. violaceum Voglino, Ann. Reale Accad.
Agric. Torino 55: 381. “1912” 1913.
Type: Italy, Liguria, Albenga, on leaves of Solanum lycopersicum
(= Lycopersicon esculentum) (Solanaceae) (location unknown).
Lit.: Saccardo (1931: 794).
Notes: Very probably synonymous with Passalora fulva.
fumago Link, Sp. pl. 6(1): 40–41. 1824 : Fr., Syst. mycol. 3(2):
372. 1832.
Type: Europe, on leaves of various plant species.
≡ Torula fumago (Link) Chevall., Fl. gén. env. Paris 1: 34. 1826.
≡ Caldariomyces fumago (Link) Woron., Ann. Mycol. 25: 261. 1926.
≡ Leptoxyphium fumago (Link) R.C. Srivast., Arch. Protistenk. 125(1–4):
333. 1982, as “(Woron.) R.C. Srivast.”, nom. inval.
= ? Fumago vagans Pers., Mycol. eur. 1: 9. 1822.
≡ Cladosporium vagans (Pers.) Desm., Pl. Crypt. N. France, Ed. 1, Fasc.
I, No. 6. 1825, as “Pers.”.
= Syncollesia foliorum C. Agardh, Syst. alg.: 32. 1824.
Lit.: Fries (1832: 372), Saccardo (1886: 547), Lindau (1910: 267),
Lind (1913: 166), Oudemans (1919–1924).
Notes: See Cladosporium vagans. Link (1824) mentioned Fumago
(“Fumago Pers. ex hoc Cladosporio saepe oritur”), but did not refer
to Fumago vagans, so that Link’s species was not based on the
latter name.
fumago f. artemisiae abrotani Thüm., Herb. Mycol. Oecon.,
Fasc. XIV, No. 657. 1879, nom. nud.
Syntype: Austria, near Kalksburg, on Artemisia abrotanum
(Asteraceae), Sep. 1879, von Thümen, Thümen, Herb. Mycol.
Oecon. 657 (e.g., B).
fumago f. corticicola Rabenh., Herb. Viv. Mycol., Ed. Nova,
Ser. Prima, Cent. IV, No. 330. 1856 and Flora 15(9): 134.
1857, nom. nud.
Syntype: Germany, Giessen, on Salix twigs (Salicaceae),
Hoffmann, Rabenhorst, Herb. Viv. Mycol. 330 (e.g., HAL).
fumago f. coryli Thüm., Herb. Mycol. Oecon., Fasc. III, No.
131. 1873, nom. nud.
Syntype: Czech Republic, Bohemia, near Teplitz-Schönau, on
leaves and twigs of Corylus avellana (Betulaceae), Jul. 1873,
Thümen, Herb. Mycol. Oecon. 131 (e.g., B).
fumago f. fragariae-vescae Thüm., Fungi Austr. Exs., Cent.
XI, No. 1085. 1874 and Herb. Mycol. Oecon., Fasc. IV, No.
169. 1874, nom. nud.
Syntypes: Czech Republic, Teplitz-Schönau, on living leaves of
Fragaria vesca (Rosaceae), summer 1873, Thümen, Fungi Austr.
Exs. 1085, Thümen, Herb. Mycol. Oecon.169 (e.g., B, HAL).
fumago f. fraxini Thüm., Herb. Mycol. Oecon., Fasc. VIII, No.
375. 1875, nom. nud.
Syntype: Czech Republic, Bohemia, near Teplitz-Schönau, on
living leaves of Fraxinus excelsior (Oleaceae), Aug. 1873, Thümen,
Herb. Mycol. Oecon. 375 (e.g., B 700006422).
Notes: Excluded, several hyphomycetes, incl. Aureobasidium
pullulans and C. herbarum (see C. vagans).
fumago f. grossulariae Thüm., Herb. Mycol. Oecon., Fasc. III,
No. 130. 1873, nom. nud.
Syntype: Czech Republic, Bohemia, near Teplitz-Schönau, on
leaves and twigs of Ribes grossularia (Grossulariaceae), Jul. 1873,
Thümen, Herb. Mycol. Oecon. 130 (e.g., B).
fumago f. humuli-lupuli Thüm., Herb. Mycol. Oecon., Fasc.
XIII, No. 606. 1878, nom. nud.
Syntype: Austria, near Klosterneuburg, on living leaves of Humulus
lupulus (Cannabaceae), Aug. 1878, Thümen, Herb. Mycol. Oecon.
606 (e.g., B).
fumago f. poae-pratensis Thüm., Herb. Mycol. Oecon., Fasc.
IV, No. 160. 1874, nom. nud.
Syntype: Czech Republic, Bohemia, near Teplitz-Schönau, on
Poa pratensis (Poaceae), summer 1873, Thümen, Herb. Mycol.
Oecon. 160 (e.g., B).
fumago f. quercus A. Walther, in herb.
Specimen: Germany, Bavaria, Bayreuth, on leaves of Quercus robur (Fagaceae),
Aug. 1877, A. Walther (BPI 426739).
Notes: Typical “Fumago vagans” (Aureobasidium pullulans and
Cladosporium sp.). See C. vagans.
fumago f. rosae-acutifoliae A. Walther, in herb.
Specimen: Germany, Bavaria, Bayreuth, on Rosa sp. (Rosaceae), Sep. 1877, A.
Walther (BPI 426751).
fumago f. carpini betuli Thüm., Herb. Mycol. Oecon., Fasc.
VII, No. 339. 1875, nom. nud.
Notes: Typical “Fumago vagans” (Aureobasidium pullulans,
Cladosporium macrocarpum and other hyphomycetes. See C.
vagans).
Syntype: Czech Republic, Bohemia, near Teplitz-Schönau, on
living leaves of Carpinus betulus (Betulaceae), Aug. 1873, Thümen,
Herb. Mycol. Oecon. 339 (e.g., B).
fumago f. rosae-albae Thüm., Herb. Mycol. Oecon., Fasc. IX,
No. 418. 1876, nom. nud.
312
the genuS Cladosporium
Syntype: Germany, Bavaria, near Bayreuth, on leaves and twigs of
Rosa ×alba (Rosaceae), Sep. 1874, Thümen, Herb. Mycol. Oecon.
418 (e.g., B).
fumago f. syringae-vulgaris Thüm., Herb. Mycol. Oecon.,
Fasc. VIII, No. 393. 1875, nom. nud.
Syntypes: Czech Republic, Bohemia, near Teplitz-Schönau, on
living leaves of Syringa vulgaris (Oleaceae), Aug. 1873, Thümen,
Herb. Mycol. Oecon. 393 (e.g., B, M-0057668).
Notes: Excluded, Trimmatostroma-like hyphomycete.
fumago f. ulmi Rabenh., Herb. Viv. Mycol., Ed. Nova, Ser.
Prima, No. 329. 1856 and Flora 15(9): 134. 1857, nom. nud.
Syntype: Germany, Dresden, on Ulmus campestris (Ulmaceae),
1856, Rabenhorst, Herb. Viv. Mycol. 329 (e.g., HAL).
fumago f. ulmi-effusae Thüm., Herb. Mycol. Oecon., Fasc. V,
No. 237. 1874, nom. nud.
Syntype: Czech Republic, Bohemia, near Bilina, on leaves and
young twigs of Ulmus sp. (Ulmaceae), Jul. 1873, Thümen, Herb.
Mycol. Oecon. 237 (e.g., B 700006428).
fumago f. vitis Thüm., Herb. Mycol. Oecon., Fasc. II, No. 76.
1872, nom. nud.
Syntype: Germany, Lübeck, near Travemünde, on Vitis vinifera
(Vitaceae), Oct. 1872, Behrens, Thümen, Herb. Mycol. Oecon. 76
(e.g., B).
fumago var. betulae L.A. Kirchn., Lotos 6: 183. 1856, nom.
nud.
Type: Czech Republic, Haasmüllnerberg near Kaplitz, on living
leaves of Betula pubescens (= B. alba) (Betulaceae) (location
unknown).
fumago “var. corticola Hoffm.”, Herb. Viv. Mycol., Ed. Nova,
Ser. Prima, No. 330. 1857, nom. nud. [see Oudemans 1920:
189].
Notes: Oudemans (l.c.) spelled the name differently, namely C.
fumago f. corticicola Rabenh., changed “f.” to “var.” and attributed
this name to “Hoffm.” although the exsiccatus has the same
number. Correct quotation → C. fumago f. corticicola.
fumago var. elongatum Mont., Flora Fernandesiana: 53.
1835.
Type: Chile, Juan Fernandez, on leaves, especially of ferns
(location unknown).
≡ Antennaria robinsonii Berk. & Mont., London J. Bot. 2: 641. 1843.
Lit.: Berkeley (1843: 640–641), Saccardo (1882: 81).
Notes: Berkeley (1843) discussed C. fumago var. elongatum and
reduced it to synonymy with Antennaria robinsonii.
fumago var. epiphyllum Rabenh. – Exsiccatus (68), speciied
in Kohlmeyer (1962: 39).
fumago var. maculaeforme Thüm., Mycoth. Univ., Cent. VII,
No. 673. 1877 and Herb. Mycol. Oecon., Fasc. 10, No. 583.
1877 and Flora 61(7): 108. 1878.
Syntypes: Germany, Bavaria, Bayreuth, on living leaves of Syringa
vulgaris (Oleaceae), autumn 1875, Thümen, Mycoth. Univ. 673
(e.g., B, HAL, MICH).
www.studiesinmycology.org
Notes: Excluded, not Cladosporium, generic afinity not yet clear.
fumago var. padi L.A. Kirchn., Lotos 6: 184. 1856, nom. nud.
Type: Czech Republic, Kaplitz, on living leaves of Padus “vulgaris”
(= ?Prunus padus) (Rosaceae) (location unknown).
fumago var. rubi L.A. Kirchn., Lotos 6: 184. 1856, nom. nud.
Type: Czech Republic, Dreisesselberg, on leaves and stems of
Rubus idaeus (Rosaceae), Jac. Jungbauer (location unknown).
fumago Mont., in Gay, Fl. chil. 8(1): 32. 1852, nom. illeg.,
homonym, non C. fumago Link, 1824.
Type: Chile, on living leaves of Eugenia (Myrtaceae) (location
unknown).
= ? Napicladium fumago Speg., Revista Fac. Agron. Vet. La Plata, Ser. 2, 6:
190. 1910.
≡ Hyphosoma fumago (Speg.) M.B. Ellis, in herb.
Lit.: Saccardo (1913a: 1396).
Notes: Status unknown.
“fungorum” Sacc., Syll. fung. 2: 406. 1883.
Type: USA, Pennsylvania, Bethlehem, on Polyporus sp.
(Polyporales).
Notes: Cited by Saccardo (1883) in the text under Sphaeria
cladosporiosa Schwein., Trans. Amer. Philos. Soc., N.S., 4(2):
211, 1832. It is unclear if Saccardo intended to introduce a new
name or if he referred to Dematium herbarum γ fungorum.
fusiforme (S.M. Reddy & Bilgrami) de Hoog & Morgan-Jones
(unpublished).
Basionym: Hyalodendron fusiforme S.M. Reddy & Bilgrami, Curr.
Sci. 40(24): 668. 1971.
≡ Retroconis fusiformis (S.M. Reddy & Bilgrami) de Hoog & Bat. Vegte,
Stud. Mycol. 31: 99. 1989.
fusisporum Berk. & M.A. Curtis, in herb.
Specimen: USA, Alabama, on stems of Chionanthus sp. (Oleaceae), ex M.A. Curtis
(NYS).
= Passalora chionanthi (Ellis & Everh.) U. Braun, Cryptog. Mycol. 20: 166.
1999.
Lit.: Braun & Schubert (2007: 65).
geniculatum Morgan-Jones, Mycotaxon 32(1): 226. 1988.
Holotype: USA, Florida, Jacksonville, Airport Holiday Inn, on
discoloured wall plaster, Dec. 1987, B.J. Jacobsen (AUA).
Ill.: Morgan-Jones & Jacobsen (1988: 227, ig. 1).
Notes: Listed as C. geniculatum Morgan-Jones & B.J. Jacobsen
by Kirk et al. (n.d.). Excluded, not a true Cladosporium, but status
not yet clear.
georginae Fuss, Archiv Vereins Siebenb. Landesk., N.F.,
14(2): 431. 1878, nom. nud.
Type: Romania, Giresau, on overwintered stems of Dahlia
pinnata (= Georgina variabilis) (Asteraceae), Fuss (location
unknown).
Notes: Classiied by Fuss (1878) as a forma of C. herbarum.
gloeosporioides G.F. Atk., Cornell Univ. Sci. Bull. 3(1): 39.
1897. Fig. 370.
Lectotype: USA, Alabama, Lee Co., Auburn, on leaves and stems of
Hypericum mutilum (Hypericaceae), 2 Sep. 1891, Duggar. (CUP-A
2170). Syntype: USA, Alabama, Lee Co., Auburn, on leaves of
313
BenSch et al.
gossypii Jacz., Khlopkovoe Delo 1929, No. 5–6: 564. 1929
and Trudy Byuro Prikl. Bot. 24(5): 181–182. 1931.
Lectotype (designated here): Uzbekistan, Bukhara, Experiment
Station, on ibres of Gossypium hirsutum (Malvaceae), 1928,
leg. V.S. Zelenetzki, det. A. Jaczewski (LEP). Isolectotype: LEP.
Other syntypes: Central Asia, on ibres of Gossypium sp., 1927,
V.S. Fedorov (LEP); l.c., 1928 (LEP); Uzbekistan, Bukhara,
Shafrikanskoje, on Gossypium hirsutum, 30 Aug. 1928, V.S.
Zelenetzki (LEP).
≡ Chalastospora gossypii (Jacz.) U. Braun & Crous, Persoonia 22: 144.
2009.
Lit.: Braun et al. (2003).
Ill.: Jaczewski (1931: 181, ig. 16).
Notes: In the publication of 1929, Jaczewski introduced the name
Cladosporium gossypii and provided a brief Russian description,
including shape and size of conidia. This description, published before
1935, is, however, valid. In his paper from 1931, he re-introduced C.
gossypii together with a Latin description and a micrograph of conidia.
Cladosporium gossypii is older than C. malorum, the basionym of
Alternaria malorum, which was published in 1931, but it cannot be
transferred to Alternaria since this name is preoccupied in this genus
by Alternaria gossypii (Jacz.) Y. Nisik. et al., 1940. See C. malorum.
gougerotii (Matr.) G. Carrión & Marg. Silva, Arch. Inst. Pasteur
Algérie 72: 532. 1955.
Neotype (selected by Borelli, 1955): USA, Memphis, isol. from
cystic lesion of human patient, (CBS 526.76).
Basionym: Sporotrichum gougerotii Matr., Compt. Rend. Hebd.
Séances Acad. Sci. 150: 545. 1910, as “gougeroti”, nom. ambig.
et dub.
≡ Rhinocladium gougerotii (Matr.) Verdun, Précis de Parasitologie
Humaine, éd. 2: 677. 1913.
≡ Dematium gougerotii (Matr.) Grigoraki, Bull. Soc. Mycol. France 40:
274. 1924, as “gougeroti”.
≡ Torula gougerotii (Matr.) Brumpt, Précis Parasitol., ed. 5: 1791. 1936.
≡ Oospora gougerotii (Matr.) D. Janke, Archiv Dermatol. Syph. 187: 693.
1949, as “Gougeroti”.
≡ Phialophora gougerotii (Matr.) Borelli, Acta Ci. Venez. 6(2): 81. 1955.
Fig. 370. Dischloridium gloeosporioides (CUP-A 2170, lectotype of C.
gloeosporioides). Conidiophores and conidia. Scale bar = 10 µm. U. Braun del.
Hypericum stans (≡ Ascyrum stans), 29 Aug. 1891, G.F. Atkinson
(CUP-A 2064).
≡ Dischloridium gloeosporioides (G.F. Atk.) U. Braun & K. Schub.,
Fungal Diversity 20: 189. 2005.
Lit.: Saccardo (1899: 1080).
Ill.: Schubert & Braun (2005b: 191, ig. 1).
gonorrhoicum Hallier, Flora, Neue Reihe, 26(19): 293. 1868.
Type: Isol. from man (gonorrhea) (location unknown).
≡ Cladosporium coniothecii-gonorrhoici Hallier, Flora, Neue Reihe,
26(19): 293. 1868 (alternative name).
Ill.: Hallier (1868a: tab. 3, igs 4, 5, 8, 9).
Notes: Undoubtedly not belonging to Cladosporium s. str. Described
as state (morph) of Coniothecium gonorrhoicum Hallier (p. 294).
“Forma penicillare. Hab. Associato a casidi gonorrhea”(Nannizzi
1934). “Species incertae” (Saccardo 1911).
314
Lit.: de Hoog & Hermanides-Nijhof (1977: 114), McGinnis & Ajello
(1982).
Notes: Of S. gougeroti: “This name has been applied to different
fungi and has therefore been abandoned.” (de Hoog et al. 2000:
1022). McGinnis & Ajello (1982) cite a lack of type material and
illustrations, and inadequate descriptions as reasons for considering
S. gougeroti a dubious name. Carrión & Silva (1955) designate No.
1792 (Kennedy Hospital, Memphis), No. 7031 (NIH ← CBS) and No.
7028 (NIH) as specimens of Cladosporium gougerotii. They illustrate
it in photomicrographs Nos. 1792 and 7028 as possessing phialides.
gramineum Link (Fries 1832) under “Species dubias”.
graminum Cooke [A. Commons 30,2060 (Sumstine 1949:
19)].
graminum f. poae-pratensis Thüm., Mycoth. Univ., Cent. V,
No. 490. 1876, nom. nud.
Syntypes: Germany, Bavaria, Bayreuth, on dry culms of Poa
pratensis (Poaceae), summer 1874, Thümen, Mycoth. Univ. 490
(e.g., B, HAL, HBG).
Notes: No Cladosporium found in several syntypes. Long
conidiophores pertain to a dematiaceous hyphomycete of uncertain
status.
the genuS Cladosporium
graminum var. scirpi Harkn., in herb.
Specimen: USA, California, “Corle Madera”(?), on dead stems of Scirpus sp.
(Cyperaceae), Apr. 1880, H.W. Harkness 1364 (BPI 426819).
Notes: Cladosporium sp. is present on the type material (but
sporulation sparse).
graminum var. sorghi Rav., in herb.
Specimen: USA, South Carolina, on Sorghum bicolor (= S. saccharatum)
(Poaceae), H.W. Ravenel (BPI 426822).
Notes: Status uncertain.
griseum (Berk. & Broome) S. Hughes, Canad. J. Bot. 31:
587. 1953.
Isotype: UK, Northamptonshire, Kings Cliff, on dead stems of Urtica
sp. (Urticaceae), Mar. 1850 (K 121543).
Basionym: Dendryphion griseum Berk. & Broome, Ann. Mag. Nat.
Hist., Ser. 2, 7: 177. 1851, as “Dendryphium”, non Polyscytalum
griseum Sacc., 1886.
≡ Polyscytalum berkeleyi M.B.
Hyphomycetes: 158. 1976, nom. nov.
Ellis,
More
Dematiaceous
Notes: Hughes (1953) apparently based his new combination and
illustration on IMI 4333.
guanicense F. Stevens, Trans. Illinois Acad. Sci. 10: 207.
1917, as “guanicensis”.
Syntypes: Puerto Rico, Guanica, on leaves of Argemone mexicana
(Papaveraceae), 2 Mar. 1913, F.L. Stevens, Porto Rican Fungi 347
(a) (e.g., BPI 426841, 426844, 426487, ILL 15873, MICH, PC).
≡ Polythrincium guanicense (F. Stevens) Cif., Ann. Mycol. 36: 233. 1938.
≡ Cercosporidium guanicense (F. Stevens) Deighton, Mycol. Pap. 112:
34. 1967.
≡ Passalora guanicensis (F. Stevens) U. Braun & R.F. Castañeda,
Cryptog. Bot. 1(1): 46. 1989.
≡ Passalora guanicensis (F. Stevens) Poonam Srivast., J. Liv. World 1(2):
116. 1994, as “guanicense”, comb. inval.
= Cercospora whetzelii Chupp, J. Dept. Agric. Porto Rico 15: 16. 1931. [Type:
CUP].
≡ Pyricularia whetzelii (Chupp) Bat. & R. Garnier, Publ. Univ. Recife Inst.
Micol. 278: 18. 1960.
Lit.: Saccardo (1931: 794), Crous & Braun (2003: 206).
gynoxidicola Petr., Sydowia 2: 381. 1948, as “gynoxidicolum”.
Fig. 371.
Types: Ecuador, Pichincha mountains near Quito, on living leaves
of Gynoxys sp. (Asteraceae), 30 Nov. 1937 [IMI 88949 (slide),
M-0057615].
≡ Stenella gynoxidicola (Petr.) J.L. Mulder, Trans. Brit. Mycol. Soc. 79(3):
478. 1982.
≡ Passalora gynoxidicola (Petr.) K. Schub. & U. Braun, Fungal Diversity
20: 200. 2005.
Lit.: Ellis (1976: 342).
Ill.: Schubert & Braun (2005b: 201, ig. 5).
harknessii (Peck) S. Hughes, Canad. J. Bot. 31: 586. 1953.
Holotype: USA, New York, Helderberg Mountains, on decaying
wood, C.H. Peck (NYS 1443). Isotype: DAOM 31921 (slide).
≡ Monilia harknessii Peck, Rep. (Annual) New York State Mus. Nat. Hist.
34: 49. 1883.
Ill.: Hughes (1953: 586, ig. 7).
Notes: Hughes (1953) apparently based his new combination and
illustration on DAOM 28997. Excluded, not Cladosporium s. str.,
a wood-inhabiting hyphomycete, cf. Parahaplotrichum idahoense.
helicosporum R.F. Castañeda & W.B. Kendr., Mycotaxon 63:
183. 1997.
www.studiesinmycology.org
Fig. 371. Passalora gynoxidicola (M-0057615, type of C. gynoxidixola).
Conidiophores and conidia. Scale bar = 10 µm. K. Bensch del.
Holotype: Cuba, La Habana, La Chorrera, on fallen leaves of
Laciasis ligulata (= L. divaricata) from a rainforest, 14 Apr. 1992,
R.F. Castañeda & B. Kendrick (INIFAT C92/207-1). Isotype: MUCL
39868.
Ill.: Castañeda et al. (1997: 184–185, igs 1–2).
Notes: Excluded, not belonging to Cladosporium s. str. Status
unclear.
hemileiae Steyaert, Bull. Soc. Roy. Bot. Belgique 63(1): 47.
1930. Fig. 372.
Holotype: Zaire, Prov. Orientale, Biaro, near Kisangani (Stanleyville),
on uredosoris of Hemileia vastatrix (Uredinales) on Coffea robusta
(Rubiaceae), Oct. 1929, R.L. Steyaert (BPI 426854).
≡ Digitopodium hemileiae (Steyaert) U. Braun, Heuchert & K. Schub.,
Schlechtendalia 13: 65. 2005.
Lit.: Saccardo (1972: 1337), Sutton (1973: 40).
Ill.: Steyaert (1930: tab. 4–5), Heuchert et al. (2005: 65, ig. 26, pl.
1, igs 4, 7).
herbarum f. petiolorum fraxini Thüm., in herb.
Specimen: Germany, Bayern, Bayreuth, on petioles of Fraxinus sp. (Oleaceae),
Apr. 1876, ex herb. Thümen (M).
Notes: Several hyphomycetes inclusive C. herbarum and a
Colletotrichum-like species (dark seta-like conidiophores with paler
apices).
herbarum f. rubi Gonz. Frag., Mem. Real Acad. Ci. Barcelona,
Ser. 3, 15(17): 458 (32). 1920.
Syntypes: Spain, Barcelona, Bonanova and Vallvidrera, on leaves
of Rubus rusticanus (= R. ulmifolius subsp. rusticanus) and Rubus
sp. (Rosaceae), Sep. 1915 and Jul. 1918, Fr. Sennen (MA 06328,
06329).
= Pseudocercospora rubi (Sacc.) Deighton, Mycol. Pap. 140: 152. 1976.
315
BenSch et al.
= ? Ascospora solidaginis Fr., Summa veg. Scand. 2: 425. 1849.
= ? Cercospora sagittariae Ellis & Kellerm., J. Mycol. 2: 1. 1886.
Notes: Synonomy and exsiccati in Oudemans (1923). Examinations
of several collections from HAL and M, all on Sagittaria sagittifolia
(e.g., Thümen, Mycoth. Univ. 1167 and Rabenhorst, Fungi. Eur.
Exs. 1468) always contained Cercospora sagittariae.
heterosporium, in herb.
Specimen: USA, New York, Buffalo, on an unidentiied host plant, G.W. Clinton (BPI
427204).
Notes: Excluded, conidiophores with tretic conidiogenous loci.
Taxonomic afinity remains unclear.
hoveae Syd. & P. Syd., Ann. Mycol. 15: 148. 1917. Fig. 373.
Holotype: Australia, Brisbane River, on leaves of Hovea sp.
(“longifolia var. pannosa”) (Fabaceae), 1863–1865, A. Dietrich,
comm. J. Bornmüller (S).
≡ Pseudocercospora hoveae (Syd. & P. Syd.) K. Schub. & U. Braun,
Mycol. Progr. 4(2): 106. 2005.
Lit.: Saccardo (1931: 792).
Ill.: Schubert & Braun (2005a: 106, ig. 6).
humile Davis, Trans. Wisconsin Acad. Sci. 19: 702. 1919.
Lectotype: USA, Wisconsin, Luck, on leaves of Acer rubrum
(Sapindaceae), 25 Aug. 1916, J.J. Davis (WIS). Isolectotype: BPI
427214.
≡ Fusicladium humile (Davis) K. Schub. & U. Braun, IMI Descriptions of
Fungi and Bacteria 152, No. 1520. 2002.
Fig. 372. Digitopodium hemileiae (BPI 426854, holotype of C. hemileiae).
Conidiophores with digitate or rhizoid protuberances and conidia. Scale bar = 10
µm. B. Heuchert del.
Lit.: Saccardo (1931: 795), Prasil & de Hoog (1988: 53, as “var.”),
Schubert & Braun (2007: 200).
herbarum f. sechii-edulis Thüm., Herb. Mycol. Oecon., Fasc.
X, No. 466. 1877, nom. nud.
Syntype: USA, South Carolina, Aiken, on faded leaves of Sechium
edule (Cucurbitaceae), autumn 1876, H.W. Ravenel, ex herb.
Thümen (M) and Thümen, Herb. Mycol. Oecon. 466 (e.g., M).
= Cercospora apii Fresen. s. lat.
Lit.: Stevenson (1971).
herbarum var. hypharum (sic) Westend. & Van Haes., Lindau
(1910 – in index).
Notes: An error. Refers to C. herbarum var. typharum Westend. &
Van Haes., in Lindau (1907: 801).
herbarum var. rubi Gonz. Frag., Mem. Real Acad. Ci.
Barcelona, Ser. 3, 15(17): 458 (32). 1920.
Notes: See C. herbarum f. rubi Gonz. Frag.
heteronemum (Desm.) Oudem., Arch. Néerl. Sci. Exact. Nat.
11: 363. 1876.
Type: France, on dead leaves of Sagittaria sagittifolia
(Alismataceae), Desmazières, Pl. Crypt. N. France, Sér. 2, No. 7.
Basionym: Macrosporium heteronemum Desm., Ann. Sci. Nat.
Bot., Sér. 3, 20: 216. 1853.
≡ Helminthosporium heteronemum (Desm.) Oudem. (in herb.? ).
316
Fig. 373. Pseudocercospora hoveae (S, holotype of C. hoveae). Conidiophores and
conidia. Scale bar = 10 µm. K. Bensch del.
the genuS Cladosporium
≡ Fusicladosporium humile (Davis) Partridge & Morgan-Jones,
Mycotaxon 85: 366. 2003.
= Venturia acerina Plakidas ex M.E. Barr, Canad. J. Bot. 46: 814. 1968.
Lit.: Saccardo (1931: 788), Ellis (1976: 340), Sivanesan (1984: 607,
as Cladosporium state of Venturia acerina), Schubert et al. (2003: 57).
inaequiseptatum Matsush., Icones Microfungorum a
Matsushima Lectorum: 35. 1975.
Holotype: Japan, Miyajima, Hiroshima, on a dead leaf of Quercus
phillyraeoides (Fagaceae), May 1972 (Matsush. Herb. 4428).
≡ Parapleurotheciopsis inaequiseptata (Matsush.) P.M. Kirk, Trans.
Brit. Mycol. Soc. 78(1): 65. 1982.
infuscans Thüm., Rev. Mycol. (Toulouse) 1: 59. 1879. Fig.
374.
Syntypes: USA, South Carolina, Aiken, on living stems of
Desmodium strictum (Fabaceae), H.W. Ravenel, Thümen, Mycoth.
Univ. 1573 (e.g., BPI 427232, 427231, HAL, M, NY); Ravenel,
Fungi Amer. Exs. 598 (e.g., NY, PH 01020439). Possible syntypes:
Roumeguère, Fungi Sel. Gall. Exs. 4788 (e.g., NY).
≡ Dendryphiella infuscans (Thüm.) M.B. Ellis, Dematiaceous
Hyphomycetes: 500. 1971.
Lit.: Saccardo (1886: 361).
jacarandae Viégas, Bragantia 7(2): 33. 1947. Fig. 375.
Holotype: Brazil, Minas Gerais, Agua Limpa, Exp. de Agua Limpa,
on living leaves of Jacaranda sp. (Bignoniaceae), 21 May 1945,
E.P. Heringer (IACM).
≡ Fusicladium jacarandae (Viégas) K. Schub., U. Braun & F. Freire,
Sydowia 56(2): 302. 2004.
Ill.: Viégas (1947: 46, ig. 11), Schubert & Braun (2004: 303, ig. 3).
jaczewskii Rojdest., in herb.
Fig. 374. Dendryphiella infuscans (WIS, lectotype of C. elsinoes). Conidia and
conidiophores. Scale bar = 10 µm. B. Heuchert del.
Specimen: Russia, Altaj, Bijskij uezd, on insect eggs from the lower side of leaves of
Prunus padus (Rosaceae), 1 Nov. 1924, N.A. Rojdestwensky (LEP).
Notes: Excluded. A mixture of conidia of several hyphomycetes
(Alternaria sp., Cladosporium cf. herbarum, Cladosporium sp.).
lactucae in herb.
Specimen: Turkestan, on leaves of Lactuca serriola (Asteraceae), 28 May 1912,
Barbarin (LEP).
= Passalora scarioliae Syd., Ann. Mycol. 34: 401. 1936.
Notes: It is a new record for Passalora scarioliae until now only
known from Germany, India and Iran.
lactucae Sawada, Rep. Gov. Res. Inst. Formosa 85: 92.
1943, nom. inval.
Syntypes: Taiwan, Taipei, on Pteroscypsela indica (≡ Lactuca
indica) (Asteraceae), 9 Mar. 1924, K. Sawada (BPI 427238, PPMH).
= Cladosporium lactucicola Y. Cui & Z.Y. Zhang, Mycosystema 21(1): 22.
2002.
≡ Cladosporium lactucicola Z.Y. Zhang & Y. Cui, Flora Fungorum
Sinicorum, Vol. 14: 114. 2003, nom. superl.
≡ Passalora lactucicola (Y. Cui & Z.Y. Zhang) K. Schub. & U. Braun,
Mycol. Progr. 4(2): 105. 2005.
Ill.: Schubert & Braun (2005a: 105, ig. 4).
Additional authentic material: Japan, Anetai, Iwate Prefecture, on
Lactuca raddeana, 20 Oct. 1947, A. Ouchi, herb. Sawada (herb.
Univ. Iwate).
lactucicola Y. Cui & Z.Y. Zhang, Mycosystema 21(1): 22.
2002. Fig. 376.
Holotype: China, Sichuan Prov., Chengdu, on living leaves of
Pteroscypsela indica (≡ Lactuca indica) (Asteraceae), 15 Aug.
1985, Y. Qing (MHYAU 03881).
www.studiesinmycology.org
Fig. 375. Fusicladium jacarandae (IACM, holotype of C. jacarandae). Conidiophores
and conidia. Scale bar = 10 µm. K. Bensch del.
317
BenSch et al.
and its anamorphs: 1. Names published in Cercospora and Passalora,
CBS Biodiversity Ser. 1: 458. 2003.
Lit.: Saccardo (1886: 358).
leproides (L. Léger & Nogueira) Nann., Trattato di
Micopatologia Umana 4: 409. 1934, as “(Léger & Nogueira)
Thom 1930”.
Type: Isol. from two patients with mild lesions resembling leprosy
(location unknown).
Basionym: Scopulariopsis leproides L. Léger & Nogueira, Bull. Soc.
Pathol. Exot. 15: 656. 1922.
≡ Hormodendrum leproides (L. Léger & Nogueira) C.W. Dodge, Med.
Mycol.: 848. 1935.
Notes: “Certainly not a Scopulariopsis” ( Mycol. Pap. 86: 87, 1963).
Not Cladosporium s. str.
lethiferum House, Bull. New York State Mus. Nat. Hist.
219/220: 62. 1919–1920.
Notes: Petrak (Petrak’s Lists 1: 192) seems to have errored.
Cladosporium lethiferum Peck is the published name. See
comments under Cladosporium brevipes House.
Fig. 376. Passalora lactucicola (BPI 427238, syntype of C. lactucae). Conidiophores
and conidia. Scale bar = 10 µm. K. Bensch del.
≡ Cladosporium lactucicola Z.Y. Zhang & Y. Cui, Flora Fungorum
Sinicorum, Vol. 14: 114. 2003, nom. superl.
≡ Passalora lactucicola (Y. Cui & Z.Y. Zhang) K. Schub. & U. Braun,
Mycol. Progr. 4(2): 105. 2005.
= Cladosporium lactucae Sawada, Rep. Gov. Res. Inst. Formosa 85: 92. 1943,
nom. inval.
Ill.: He & Zhang (2002: 22, ig. 2), Zhang et al. (2003: 114, ig. 73),
Schubert & Braun (2005a: 105, ig. 4).
ladinum E. Müll., Sydowia 4: 294. 1950.
Type: Switzerland, Graubünden, Scuol, on dead stems of
Laserpitium halleri (Apiaceae), 17 Jul. 1948, E. Müller (ZT).
= ? Leptosphaeria ladina E. Müll., Sydowia 4: 293, 1950.
≡ Nodulosphaeria ladina (E. Müll.) L. Holm, Symb. Bot. Upsal. 14(3): 83,
1957.
Notes: “It must be considered a culture contaminant rather than an
anamorph” (Crane & Shearer 1991).
lantanae K. Bhalla & A.K. Sarbhoy, Indian Phytopathol.
53(3): 263. 2000, as “(Deighton) K. Bhalla & A.K. Sarbhoy
comb. nov.”, nom. illeg. [based on Mycovellosiella lantanae
var. cubensis Deighton, non Cladosporium cubense R.F.
Castañeda, 1987, but the circumscription included C.
trichophilum Petr.].
Type: Cuba, Bayamo, on living leaves of Lantana camara
(Verbenaceae), Mar. 1967, R. Urtiaga (IMI 126781b).
≡ Mycovellosiella lantanae Chupp var. cubensis Deighton, Mycol. Pap.
137: 36. 1974.
≡ Passalora lantanae var. cubensis (Deighton) U. Braun & Crous,
Mycosphaerella and its anamorphs: 1. Names published in Cercospora
and Passalora, CBS Biodiversity Ser. 1: 243. 2003.
laxum Kalchbr. & Cooke, Grevillea 9(49): 24. 1880.
Types: South Africa, on fading leaves of Printzia pyrifolia
(Asteraceae) (IMI 115272, K).
≡ Mycovellosiella laxa (Kalchbr. & Cooke) Deighton, Mycol. Pap. 137: 65.
1974.
≡ Passalora laxa (Kalchbr. & Cooke) U. Braun & Crous, Mycosphaerella
318
lethiferum Peck, Rep. (Annual) New York State Mus. Nat.
Hist. 40: 64. 1887.
Holotype: USA, New Hampshire, Keene, on living leaves of
Populus tremuloides (Salicaceae), Jun. 1887, Peck (NYS). Isotype:
BPI 427241.
≡ Pollaccia lethifera (Peck) M. Morelet, Bull. Soc. Sci. Nat. Archéol.
Toulon & Var 34(219): 12. 1978.
≡ Pollaccia radiosa (Lib.) E. Bald. & Cif. var. lethifera (Peck) M. Morelet,
Cryptog. Mycol. 6: 113. 1985.
≡ Fusicladium radiosum (Lib.) Lind var. lethiferum (Peck) Ritschel & U.
Braun, Schlechtendalia 9: 87. 2003.
= Clasterosporium populi Ellis & Everh., J. Mycol. 7: 134. 1892, nom. illeg.,
homonym, non C. populi Sacc., 1886. [Type: NY].
≡ Stigmina populi Pound & Clem., Bull. Geol. Nat. Hist. Surv. 9: 662.
1896, as “(Ellis & Everh.) Pound & Clem.”.
≡ Stigmina populi Peck, Bull. New York State Mus. Nat. Hist. 157: 34.
1912, as “(Ellis & Everh.) Peck”.
= Dicoccum populinum Ellis & Everh., Proc. Acad. Nat. Sci. Philadelphia 1893:
462. 1894. [Type: NY].
= Fusicladium lageniforme Solheim & Hadield, Univ. Wyoming Publ. 1946:
18–19. 1946, nom. inval.
= Pollaccia americana Ondřej, Eur. J. Forest Pathol. 2: 144. 1972. [Type:
DAOM, as Fusicladium radiosum (Lib.) Lind].
= Venturia tremulae Aderh. var. grandidentatae M. Morelet, Cryptog. Mycol.
6: 113. 1985.
Lit.: Saccardo (1892: 604).
levieri (Magnus) Hara, Agric. & Hort. 12: 2706. 1937.
Holotype: Caucasus, Georgia, Batum, “in silvis litoris Euscini”, on
Diospyros lotus (Ebenaceae), 16 Jun. 1890 (HBG).
≡ Fusicladium levieri Magnus, Trudy Imp. S.-Peterburgsk. Bot. Sada
16: 543. 1900.
≡ Ragnhildiana levieri (Magnus) Vassiljevsky, in Vassiljevsky & Karakulin,
Parazitnye nesovershennye griby, Ch. 1., Gifomitsety: 373. 1937.
≡ Phaeoramularia levieri (Magnus) U. Braun, Proc. Komarov Bot. Inst.
(St. Petersburg) 20: 68. 1997.
= Fusicladium kaki Hori & Yoshino, Bot. Mag. (Tokyo) 19: 220. 1905.
= Fusicladium diospyros Chona, Munjal & J.N. Kapoor, Indian Phytopathol. 9:
129. 1956. [Type: HCIO?].
= Fusicladium diospyros Hori & Yoshino, in herb. (B).
Lit.: Schubert (2001), Crous & Braun (2003: 482), Schubert et al.
(2003: 61–62), Scholler et al. (2004: 132–134).
lichenicola Linds., Quart. J. Microscop. Sci., N.S., 11: 42.
1871, as “lichenicolum”, nom. inval.
the genuS Cladosporium
Type: UK, Scotland, S. Aberdeenshire, Falls of the Garrawalt, on
thallus of Peltigera aphthosa (Peltigeraceae), Aug. 1856, W.L.
Lindsay.
Lit.: Hawksworth (1979: 269), Heuchert et al. (2005: 55).
Notes: “...refers only to sterile mycelium. Lindsay was hesitant in
introducing the name as he stated that the fungus ‘... if it is entitled
to speciic distinction, may be itly denominated C. lichenicolum’.”
(Hawksworth 1979). The original description of this species is
insuficient and type material is not preserved so the generic afinity
of this species remains unknown. Hawksworth (1979) considered
this name as probably invalid, according to ICBN, Art. 34, a
conclusion conirmed by examination of the original description.
lichenum Keissl., Centralbl. Bakteriol., 2. Abth., 37: 389.
1913.
Holotype: Austria, Steiermark, valle See-Aü at Leopoldsteiner See
near Eisenerz, alt. 700 m, on apothecia of Loxospora cismonica (≡
Haematomma cismonicum) (Sarrameanaceae), Jul. 1912, K. von
Keissler (W1912/117).
≡ Pseudocercospora lichenum (Keissl.) D. Hawksw., Bull. Brit. Mus. (Nat.
Hist.), Bot. 6(3): 246. 1979.
Lit.: Saccardo (1931: 796), Heuchert et al. (2005: 69).
Ill.: Hawksworth (1979: 247, Fig. 31).
Notes: Excluded, status still unclear, but based on unthickened
conidiogenous loci in any case not a species of Cladosporium s. str.
lignatile Schwein., Trans. Amer. Philos. Soc., N.S., 4(2): 277.
1832.
Fig. 377. Zasmidium lonicericola (BPI 427243, syntype of C. lonicerae).
Conidiophores and conidia. Scale bar = 10 µm. K. Bensch del.
Holotype: USA, Pennsylvania, Bethlehem, on rotten wood, No.
2601 (PH 01020417, as “lignatilis”).
≡ Virgaria lignatilis (Schwein.) S. Hughes, Canad. J. Bot. 31: 603. 1953.
= Botrytis nigra Link, Ges. Naturf. Freunde Berlin Mag. Neuesten Entdeck.
Gesammten Naturk. 3: 14. 1809.
≡ Virgaria nigra (Link) Nees, Syst. Pilze 1: 54. 1817.
≡ Sporotrichum nigrum (Link) Fr., Syst. mycol. 3(2): 416. 1832.
≡ Trichosporum nigrum (Link) Fr., Summa veg. scand. 2: 492. 1849.
= Sporotrichum fuliginosum Pers., Mycol. eur. 1: 77. 1822. [Type: L].
= Botrytis atrofumosa Cooke & Ellis, Grevillea 6(39): 90. 1878. [Types K, NY].
≡ Virgaria atrofumosa (Cooke & Ellis) Sacc., Syll. fung. 4: 281. 1886.
= Trichosporum splenicum Sacc. & Berl., Atti Reale Ist. Veneto Sci. Lett. Arti,
Ser. 6, 3(4): 741. 1885. [Type: PAD].
Lit.: Saccardo (1886: 356), Hughes (1958: 751, 823).
lonicerae Sawada, Rep. Gov. Res. Inst. Formosa 86: 163.
1943, nom. inval.
Syntype: Taiwan, Taipei, on Lonicera japonica var. sempervillosa
(Caprifoliaceae), 20 Dec. 1914, K. Sawada (BPI 427243).
= Zasmidium lonicericola (Y.H. He & Z.Y. Zhang) Crous & U. Braun,
Persoonia 23: 140. 2009.
lonicericola Y.H. He & Z.Y. Zhang, Mycosystema 20(4): 469.
2001 and in Zhang et al., Flora Fungorum Sinicorum, Vol. 14:
116. 2003. Figs 377, 378.
Holotype: China, Yunnan Prov., Kunming, on living leaves of
Lonicera japonica (Caprifoliaceae), 3 Aug. 1990, H. Li (MHYAU
03533). Epitype (designated in Crous et al., 2009c): South Korea,
Hongchon, on leaves of Lonicera japonica, 30 Oct. 2004, coll. H.-D.
Shin, isol. P.W. Crous (CBS H-20271) (dried culture). Ex-epitype
cultures: CPC 11671 = CBS 125008, CPC 11672, 11673.
Fig. 378. Zasmidium lonicericola (HAL). Conidiophores and conidia. Scale bar = 10
µm. K. Bensch del.
Ill.: He & Zhang (2001: 469, ig. 1), Zhang et al. (2003: 117, ig. 75),
Schubert & Braun (2005b: 205–206, igs 7–8).
Notes: Based on its ITS DNA phylogeny and scar type (planate
instead of pileate), this species is better placed in Zasmidium
(Crous et al. 2009c). Abelia bilora and Leycesteria formosa
(Caprifoliaceae) are reported as additional hosts. Cladosporium
≡ Stenella lonicericola (Y.H. He & Z.Y. Zhang) K. Schub., H.D. Shin & U.
Braun, Fungal Diversity 20: 204. 2005.
≡ Zasmidium lonicericola (Y.H. He & Z.Y. Zhang) Crous & U. Braun,
Persoonia 23: 140. 2009.
www.studiesinmycology.org
319
BenSch et al.
lonicerae Sawada is cited as synonym, which could be conirmed
based on a re-examination.
machili Sawada, Special Publ. Coll. Agric. Natl. Taiwan Univ.
8: 196. 1959, nom. inval.
lysimachiae H.C. Greene, Trans. Wisconsin Acad. Sci. 38:
232. 1946, nom. illeg., homonym, non C. lysimachiae Guba,
1939.
Holotype: Taiwan, Pref. Taipei, Taipei, on leaves of Machilus
thunbergii (Lauraceae), 15 Nov. 1914, Y.F (PPMH).
Notes: Excluded, taxonomic status remains unclear.
Gonatophragmium?
Lectotype (designated here): USA, Wisconsin, Dane Co.,
Madison, Univ. Wisconsin Arboretum, Marsh, on living leaves and
stems of Lysimachia terrestris (Primulaceae), 24 Aug. 1943, H.C.
Greene (WIS). Isotype: BPI 427245).
= Fusicladium lysimachiae (Guba) K. Schub. & U. Braun, Mycol. Progr. 4(2):
102. 2005.
lysimachiae Guba, Rhodora 41: 513. 1939. Fig. 379.
Holotype: USA, Massachusetts, Nantucket Co., Nantucket, in
waste places near the waterfront east of Main Street, on living
leaves, rarely on the stems of Lysimachia vulgaris (Primulaceae),
15 Aug. 1936, E.F. Guba, No. 115 (ILL 21101).
≡ Fusicladium lysimachiae (Guba) K. Schub. & U. Braun, Mycol. Progr.
4(2): 102. 2005.
= Cladosporium lysimachiae H. C. Greene, Trans. Wisconsin Acad. Sci. 38:
232. 1946, nom. illeg.
Ill.: Schubert & Braun (2005a: 103, ig. 1).
lythri Westend., Bull. Acad. Roy. Sci. Belgique 21(8): 240.
1854.
Type: Belgium, near Courtrai, on leaves of Lythrum salicaria
(Lythraceae), Westendorp, No. 1091 (BR).
≡ Cercospora lythri (Westend.) Niessl, Hedwigia 15: 1. 1876.
≡ Stenella lythri (Westend.) J.L. Mulder, Trans. Brit. Mycol. Soc. 65: 517.
1975.
≡ Zasmidium lythri (Westend.) U. Braun & H.D. Shin, comb. nov.
MycoBank, MB800302.
= Cercospora sanguinea Fuckel, Hedwigia 5: 30. 1866. [Type: Fuckel, Fungi
Rhen. Exs. 1630, e.g., HAL].
Lit.: Saccardo (1886: 452), Lindau (1910: 122, 803), Chupp (1954:
362), Crous & Braun (2003: 259).
maculatum Cooke, Fungi Brit. Exs., No. 162 (?).
Syntype: UK, Channel Islands, Jersey, on an unidentiied host,
Cooke, Fungi Brit. Exs. 162 (e.g., B 700006613).
Lit.: Oudemans (1921).
Notes: Excluded. Material immature and poor, cercosporoid.
maculicola Ellis & Barthol., in herb.
Specimen: USA, Kansas, Rockport, on leaves of Populus deltoids subsp. monilifera
(≡ P. monilifera) (Salicaceae), Sep. 1894, E. Bartholomew, ex herb. Ellis (BPI
427256, type material of Cladosporium subsessile).
≡ Fusicladium subsessile (Ellis & Barthol.) K. Schub. & U. Braun, IMI
Descriptions of Fungi and Bacteria 152, No. 1519. 2002.
maculicola (Romell & Sacc.) M. Morelet, Bull. Soc. Sci. Nat.
Archéol. Toulon & Var 201: 4. 1972, as “maculicolum”.
Lectotype: Sweden, Nacka Vikdalen, on living leaves of Populus
tremula (Salicaceae), 24 Jun. 1890, L. Romell (S). Isolectotypes:
IMI 17008, PAD, WINF(M) 11082.
Basionym: Torula maculicola Romell & Sacc., Grevillea 21(99): 69.
1893, non Fusicladium maculicola (Ellis & Kellerm.) Ondřej, 1973.
≡ Phaeoramularia maculicola (Romell & Sacc.) B. Sutton, Canad. J. Bot.
48: 471. 1970.
≡ Fusicladium romellianum Ondřej, Česká Mykol. 27(4): 237. 1973.
= Pollacia borealis A. Funk, Canad. J. Bot. 67: 776. 1989. [Type: DAVFP 23609].
Lit.: Ellis (1976: 322), Schubert & Braun (2002b, c), Schubert et al.
(2003: 90–92).
madagascarense Sartory, Champignons Parasites de
l’Homme et des Animaux 11: 729. 1923, as “madagascarensis”.
Type: Madagascar, infection of human.
≡ Cladosporium sp. (“madagascariensis”) Guég., Compt. Rend. Hebd.
Séances Acad. Sci. 152(7): 412–413. 1911.
≡ ? Cladosporium madagascariense Verdun, Précis de Parasitologie
Humaine, éd. 2: ?. 1912 [publication not found].
≡ Hormodendrum madagascarense (Verdun) C.W. Dodge, Med. Mycol.:
845. 1935.
Lit.: de Hoog et al. (2000: 1025).
Notes: The name C. madagascariensis was not coined by
Guéguen (C. sp.). A description for this species was given in
Sartory (1923: 729), who cited “Verdun, 1912” without any details.
But this publication could not be traced, and it is unclear if Verdun
introduced the name C. madagascarense and if he published a irst
description. Doubtful species, no material known (de Hoog et al.
2000).
malopis Fuss, Archiv Vereins Siebenb. Landesk., N.F., 14(2):
431. 1878, nom. nud.
Type: Romania, Giresau, on overwintered stems of Malope
malacoides (Malvaceae), Fuss (location unknown).
Notes: Classiied by Fuss (1878) as a forma of C. herbarum.
malorum Ruehle, Phytopathology 21: 1146. 1931.
Type: USA, Washington, on living fruits of Malus sylvestris (= Pyrus
malus) (Rosaceae).
Fig. 379. Fusicladium lysimachiae (ILL 21101, holotype of C. lysimachiae).
Conidiophores and conidia. Scale bar = 10 µm. K. Bensch del.
320
≡ Cladosporium malorum Heald & Ruehle, Wash. State Agric. Exp. Sta.
Bull. 245: 48. 1930, nom. nud.
≡ Alternaria malorum (Ruehle) U. Braun, Crous & Dugan, Mycol. Progr.
the genuS Cladosporium
2(1): 5. 2003.
= Cladosporium gossypii Jacz., Khlopkovoe Delo 1929, 5–6: 564. 1929 and
Trudy Byuro Priklad. Bot. 24 (5): 181–182. 1931.
≡ Chalastospora gossypii (Jacz.) U. Braun & Crous, Persoonia 22: 144.
2009.
= Phaeoramularia kellermaniana Marasas & I.H. Bredell, Bothalia 11: 217.
1974 [Types: IMI 165252, PREM 44703].
≡ Cladophialophora kellermaniana (Marasas & I.H. Bredell) U. Braun &
Feiler, Microbiol. Res. 150: 83. 1995.
≡ Pseudocladosporium kellermaniana (Marasas & I.H. Bredell) U. Braun,
A monograph of Cercospora, Ramularia and allied genera 2: 393. 1998.
= Cladosporium porophorum Matsush., Icones Fungorum a Matsushima
Lectorum: 36. 1975.
Lit.: Ho et al. (1999: 134), Goetz & Dugan (2006).
Notes: Due to the phylogenetic position of this taxon within the
Pleosporaceae close to Alternaria spp. and the morphologically
similarity to Alternaria cetera Braun et al. (2003) assigned C.
malorum to Alternaria s. lat. However, the small, amero- to
phragmosporous conidia of A. cetera as well as A. malorum are
rather different from those of common Alternaria species. In a
recently published manual of Alternaria, Simmons (2007) excluded
A. cetera from the latter genus and placed it in the new genus
Chalastospora. Cladosporium malorum agrees with the concept of
this genus and is congeneric (Crous et al. 2009a). Type material
of C. gossypii was re-examined and it is identical to C. malorum.
However, C. gossypii is an older name than C. malorum, which was
published in 1931, and has priority.
mansonii (Castell.) Pinoy, Ann. Dermatol. Syphiligr., Sér. 5, 3:
341. 1912 ? (not on page 341).
Neotype (selected by de Hoog & Hermanides-Nijhof, 1977): India,
Ceylon [Sri Lanka], isol. from a human patient (CBS H-7131).
Isotypes: CBS H-7132, CBS H-7133. Ex-neotype culture: CBS
158.58 = IMI 134457 = ATCC 18657.
≡ Microsporum mansonii Castell., Brit. Med. J. 2: 1271. 1905.
≡ Foxia mansonii (Castell.) Castell., J. Trop. Med. Hyg. 11: 261. 1908.
≡ Malassezia mansonii (Castell.) Verdun, Précis de Parasitologie
Humaine, éd. 2: 698. 1912.
≡ Torula mansonii (Castell.) Vuill., Compt. Rend. Hebd. Séances Acad.
Sci. 89: 406. 1929.
≡ Sporotrichum mansonii (Castell.) Toro, Sci. Surv. Porto Rico & Virgin
Islands 8: 222. 1932, as “mansoni”.
≡ Dematium mansonii (Castell.) C.W. Dodge, Med. Mycol.: 678. 1935.
≡ Pullularia mansonii (Castell.) Borelli, Riv. Anat. Patol. Oncol. 17: 617.
1960.
≡ Aureobasidium mansonii (Castell.) W.B. Cooke, Mycopathol. Mycol.
Appl. 17: 34. 1962.
≡ Rhinocladiella mansonii (Castell.) Schol-Schwarz, Antonie van
Leeuwenhoek J. Microbiol. Serol. 34: 122. 1968.
≡ Exophiala mansonii (Castell.) de Hoog, Stud. Mycol. 15: 114. 1977.
≡ Wangiella mansonii (Castell.) Bièvre & Mariat, Bull. Soc. Franç. Mycol.
Méd. 8(2): 127. 1979.
Lit.: Nannizzi (1934: 405), Kwon-Chung & Bennett (1992: 191–
192), de Hoog et al. (2000: 1026).
Notes: De Hoog & Hermanides-Nijhof (1977) and “Index
fungorum” give “Castell. & Chalm.” as authors of the combination
Cladosporium mansonii (Castell.), Manual of Tropical Medicine,
ed. 2: 1100, 1913. Kwon-Chung & Bennett (1992) summarise
nomenclatural controversies surrounding the name C. mansonii.
According to these authors, it was Castellani who proposed the
combination C. mansonii and attributed it to Pinoy. This name
has been abandoned because of confusion. The neotype strain,
CBS 158.58, is also the ex-type strain of Exophiala castellanii (de
Hoog et al. 2000).
marinum A.K. Pal & Purkay., J. Mycopathol. Res. 30(2): 175.
1992.
www.studiesinmycology.org
Holotype: India, West Bengal, Sundarban, Bakkhali, on living
leaves of Avicennia marina (Acanthaceae), 10 Jun. 1991 (on the
label) / 14 May 1991, A.K. Pal (IMI 351331).
Ill.: Pal & Purkayastha (1992: 174, igs 3–5).
Notes: Not Cladosporium s. str., but status unclear.
martianofianum Thüm., Byull. Moskovsk. Obshch. Isp. Prir.,
Otd. Biol. 55(1): 74. 1880.
Lectotype: Russia, Siberia, Minussinsk, near river Jenissei, on
living leaves of Populus laurifolia (Salicaceae), Aug. 1879, N.
Martianoff (M). Isolectotypes: Thümen, Mycoth. Univ. 2067.
≡ Fusicladium martianofianum (Thüm.) K. Schub. & U. Braun, IMI
Descriptions of Fungi and Bacteria 152, No. 1515. 2002.
= Fusicladium asiaticum Ondřej, Česká Mykol. 27(4): 237. 1973. [Type: LE
161361].
Lit.: Saccardo (1886: 357), Lindau (1907: 818, 1910: 796), Schubert
et al. (2003: 64–65).
Ill.: Schubert et al. (2003: 65, ig. 31).
metaniger (Castell.) Ferraris, Atti Ist. Bot. “Giovanni Briosi”
3: 183. 1932.
Type: Isol. from a case of “trichomycosis nigra” on human skin,
Castellani.
Basionym: Cryptococcus metaniger Castell., Archiv Dermatol.
Syph. 16(4): 402. 1927.
= Hortaea werneckii (Horta) Nishim. & Miyaji, Jap. J. Med. Mycol. 26(2): 145.
1984.
Lit.: Nannizzi (1934: 406), Dodge (1935: 675), Cooke (1962: 27),
Kwon-Chung & Bennett (1992: 195).
Notes: In Unesco (1955: 38) as “metanigrum”. See C. werneckii.
mikaniae F. Stevens, Trans. Illinois Acad. Sci. 10: 208. 1917.
Syntypes: Puerto Rico, Las Marias, on leaves of Mikania sp.
(Asteraceae), 22 Mar. 1913, F.L. Stevens (ILL 314, IMI 119607).
≡ Mycovellosiella mikaniae (F. Stevens) Deighton, Mycol. Pap. 137: 45.
1974.
≡ Passalora mikaniae (F. Stevens) U. Braun & F. Freire, Cryptog. Mycol.
23: 300. 2002.
Lit.: Saccardo (1931: 790), Crous & Braun (2003: 460).
milii Syd., Ann. Mycol. 12: 538. 1914.
Syntypes: France, “Lothringen”, “Wald am Oetinger Tälchen” near
Forbach, on leaves of Milium effusum (Poaceae), 22 Jun. 1913,
A. Ludwig, Sydow, Mycoth. Germ. 1295; “Ostpreussen, Warnicken,
Samland”, on leaves of Millium effusum, 11 Jul. 1914, H. Sydow,
Sydow, Mycoth. Germ. 1296 (e.g., BPI 427263, HBG, M).
≡ Passalora milii (Syd.) G.A. de Vries, Contr. Knowl. Genus
Cladosporium: 94. 1952.
Lit.: Saccardo (1931: 792), Crous & Braun (2003: 460).
minor R.F. Castañeda, Fungi Cubensis III: 22. 1988, as
“minus”, nom. illeg., homonym, non C. minor Spreng. 1827.
Holotype: Cuba, Prov. Pinar del Río, Viñales, on an unidentiied
leaf, 25 Sep. 1987, R.F. Castañeda (INIFAT C87/292-2).
≡ Alysidium minus R.F. Castañeda & W.B. Kendr., Univ. Waterloo Biol.
Ser. 35: 6. 1991, nom. nov., as “(R.F. Castañeda) R.F. Castañeda & W.B.
Kendr.”.
≡ Castanedaea minor A. Baker & Partridge, Mycotaxon 78: 178. 2001,
nom. nov., as “(R.F. Castañeda) A. Baker & Partridge”.
minor Spreng., Syst. veg. 4(1): 553. 1827, as “minus”, nom.
superl.
Type: On fallen twigs and on stems of herbaceous plants.
= Helminthosporium nanum Nees, Syst. Pilze 1: 67. 1817, as “Helmisporum
nanum Link”.
321
BenSch et al.
≡ Dendryphion nanum (Nees) S. Hughes, Canad. J. Bot. 36: 761. 1958.
Notes: Cited in Lindau (1910: 40). Type material or any other
collections unknown. Cladosporium minor is a superluous name
since Helminthosporium nanum was cited as synonym.
minourae Iwatsu, Mycotaxon 20(2): 523. 1984.
Holotype: Japan, Chiba, Shiroi, from rotting wood, 11 Feb. 1979,
T. Iwatsu (IFM 4700, Depart. Pathog. Fungi, Research Inst.
Chemobiodynamics, Chiba, Japan). Ex-type culture: ATCC 52853
= CBS 556.83 = IMI 298056).
≡ Cladophialophora minourae (Iwatsu) Haase & de Hoog, Stud. Mycol.
43: 94. 1999.
Lit.: Ho et al. (1999: 134).
minus R.F. Castañeda → minor R.F. Castañeda.
minus Spreng. → minor Spreng.
modestum Syd., Ann. Mycol. 37: 252. 1939.
Type: Sierra Leone, Kenema, on living leaves of Anthostema
senegalense (Euphorbiaceae), 5 Dec. 1938, F.C. Deighton (IMI
7520).
≡ Denticularia modesta (Syd.) Deighton, Trans. Brit. Mycol. Soc. 59(3):
422. 1972.
Lit.: Ellis (1976: 183).
molle Cooke, Grevillea 6(40): 139. 1878.
Syntypes: USA, South Carolina, Aiken, on under surface of dead
leaves of Asclepias (Apocynaceae) [IMI (slide), K, M].
≡ Cercospora molle (Cooke) Deighton, in herb. (IMI).
= Cercospora venturioides Peck, Rep. (Annual) New York State Mus. Nat.
Hist. 34: 47. 1881. [Type: NYS].
≡ Mycovellosiella venturioides (Peck) U. Braun, Proc. Komarov Bot. Inst.
(St. Petersburg) 20: 99. 1997.
≡ Passalora venturioides (Peck) U. Braun & Crous, Mycosphaerella
and its anamorphs: 1. Names published in Cercospora and Passalora,
CBS Biodiversity Ser. 1: 419. 2003.
= Cercospora illionensis Barthol., Fungi Columb., No. 2611. 1908. [Types:
Bartholomew, Fungi Columb. 2611, e.g., HBG, NY].
Fig. 380. Fusicladium monardae (WIS, lectotype of C. monardae). Conidia,
conidiophores arising from hyphae and stromata. Scale bar = 10 µm. K. Bensch del.
Lit.: Saccardo (1886: 363), Lindau (1907: 828), Crous & Braun
(2003: 419).
monardae H.C. Greene, Amer. Midl. Naturalist 50(2): 508.
1953. Fig. 380.
Lectotype (designated here): USA, Wisconsin, Dane Co. Madison,
Univ. Wisconsin Arboretum, Prairie, on living leaves of Monarda
punctata (Lamiaceae), 10 Aug. 1952, H.C. Greene (WIS). Isotype:
BPI 427271.
≡ Fusicladium monardae (H.C. Greene) K. Schub. & U. Braun,
Mycotaxon 92: 71. 2005.
Ill.: Schubert (2005a: 70, ig. 8).
murorum Petr., Ann. Naturhist. Mus. Wien 52: 288. 1941.
Syntypes: Austria, Wien, on a kitchen wall, Apr. 1941, H. Lohwag,
Krypt. exs. 3468 (B 700006638, M-00576779).
Lit.: Riedl (1968).
Notes: Taxonomic status unclear. The conidiogenous loci and
conidial hila are truncate, unthickened and not darkened, i.e.
they are not coronate as in Cladosporium. This species is
Cladophialophora-like.
musae E.W. Mason, Mycol. Pap. 13: 2. 1945. Fig. 381.
Type: Jamaica, on leaves of Musa sp. (Musaceae), 7 Sep. 1942,
E.B. Martyn [IMI 7521 (slide), ex type collection].
322
Fig. 381. Metulocladosporiella musae (IMI 7521, slide). Conidiophores and conidia.
Scale bar = 10 µm. K. Bensch del.
the genuS Cladosporium
≡ Periconiella sapientumicola Siboe, African J. Mycol. Biotechnol. 1994:
4. 1994, non Periconiella musae M.B. Ellis, 1967.
≡ Metulocladosporiella musae (E.W. Mason) Crous, Schroers,
Groenewald, U. Braun & K. Schub., Mycol. Res. 110: 269. 2006.
Lit.: Ellis (1971: 317), David (1988c), Ho et al. (1999: 136).
Ill.: Martyn (1945: 3, ig. 1), Ellis (1971: 318, ig. 219 A), David
(1988c: 1, ig.), Ho et al. (1999: 138, igs 35–37), Crous et al. (2006:
269–272, igs 3–14).
myrticola Bubák, Ann. Mycol. 13: 113. 1915, as “myrticolum”.
Fig. 382.
Holotype: Italy, Tyrol, Gries near Bozen, on leaves of Myrtus
communis (Myrtaceae), 30 May 1914, Dr. W. Pfaff (BPI 427273).
≡ Fusicladium myrticola (Bubák) K. Schub. & U. Braun, Fungal
Diversity 20: 197. 2005.
Lit.: Saccardo (1931: 793).
Ill.: Schubert & Braun (2005b: 199, ig. 4).
Notes: On the packet of the type specimen, the month of the
collecting date is given as June.
myrticola R.F. Castañeda & W.B. Kendr., Univ. Waterloo Biol.
Ser. 35: 20. 1991, nom. illeg., homonym, non C. myrticola
Bubák, 1915.
Holotype: Cuba, Pinar del Río, Cuchillas de San Simón, on dead
leaves of Syzygium jambos (Myrtaceae), 24 Mar. 1990, R.F.
Castañeda (INIFAT C90/108).
Ill.: Castañeda & Kendrick (1991: 22, ig. 11).
Notes: Excluded, but generic afinity unclear.
nervale Ellis & Dearn., in Bartholomew, Fungi Columb., Cent.
21, No. 2010. 1905.
Syntypes: Canada, London, on living leaves of Rhus typhina
(Anacardiaceae), Jul./ Aug. 1904, J. Dearness, Bartholomew,
Fungi Columb. 2010 (e.g., BPI 427277–427278, ILL, NY).
= Cladosporium aromaticum Ellis & Everh., Proc. Acad. Nat. Sci. Philadelphia
1895: 439. 1895.
≡ Fusicladium aromaticum (Ellis & Everh.) K. Schub. & U. Braun,
Fungal Diversity 20: 192. 2005.
Lit.: Saccardo (1913a: 1367, 1931: 788), Cash (1952: 69), Zhang
et al. (1999b: 42).
Notes: Zhang et al. (1999b) reported this species from China as
causal agent of leaf spots on Rohdea japonica (Liliaceae), which
is doubtful.
obtectum Roth – listed by Gola (1930: 21).
occultum Ces., Atti Accad. Sci. Fis. 8(4): 25. 1879.
Type: Borneo, Pulo-Pinang, on hidden glumes of Sporobolus sp.
(Poaceae), May 1865, C.O. Beccari.
≡ Dactylosporium occultum (Ces.) Cif. & Vegni, Riv. Patol. Veg., Sér.
3, 3: 207. 1963.
Lit.: Saccardo (1886: 364).
Notes: Excluded. Two specimens in Cesati collection at RO (Palmer
Marchi, pers. comm.).
oleacinum – Sacc., Syll. fung. 14: 900. 1899.
Notes: (= ? Chaetophoma oleacina Cooke). Under description of
Ch. oleacina: “Peritheciis (sic) gregariis, subglobosis, inter hyphas
fuligineas … cladosporioideas, conidia fuligenea, uniseptata,
8,4–10,5 x 3½–6 gerentes (Cladosporium oleacinum) oriundis, …;
sporulis ovoideis, 2,3–2,6 x 1–1,3 …” etc.
oleae Ellis & Everh., in herb.
Specimen: USA, California, Santa Barbara, on Olea europaea (Oleaceae), 1 Jun.
1895, J.J. Davis (WIS).
Notes: Not Cladosporium, hyphomycete of unclear identity.
oligocarpum Corda, Icon. fung. 1: 14. 1837.
Holotype: Czech Republic, near Reichenberg, on rotting wood
(PRM 155428).
≡ Didymotrichum oligocarpum (Corda) Bonord., Handb. Mykol.: 89. 1851.
Lit.: Saccardo (1886: 352), Lindau (1907: 810).
Ill.: Corda (1837: tab. 4, ig. 208).
Notes: Excluded, not Cladosporium s. str., but status still unclear.
oligocarpum var. malvacearum Berk., J. Linn. Soc., Bot. 14:
353. 1875.
Type: Cape-Verdes, St. Vincent, on leaves of some malvaceous
plant, Aug. 1873.
Lit.: Saccardo (1886: 352).
Notes: This seems to be an error in Saccardo (1886), because
Berkeley (1875) did not describe a new variety, but rather
considered it to be C. oligocarpum.
opacum Schulzer & Sacc. – cited in Oudemans (1919, 1924).
Notes: “Cladosporium opacum Schulzer & Sacc.” appears to be an
error in Oudemans (1919, 1924). Cladotrichum opacum Schulzer &
Sacc., Hedwigia 23: 127. 1884 was intended.
orbiculans Schwein., in herb.
Specimen: Surinam, on leaves of Ixora sp. (Rubiaceae) (PH 01020416).
Notes: Excluded, not Cladosporium, but status unclear.
orbiculatum Desm., Ann. Sci. Nat. Bot., Sér. 3, 11: 275. 1849.
Fig. 382. Fusicladium myrticola (BPI 427273, holotype of C. myrticola).
Conidiophores and conidia. Scale bar = 10 µm. K. Bensch del.
www.studiesinmycology.org
Lectotype (designated here): On living leaves of Sorbus domestica
(Rosaceae), herb. Desmazières (PC).
≡ Fusicladium orbiculatum (Desm.) Thüm., Fungi Austr. Exs., Cent. VIII,
323
BenSch et al.
No. 774. 1873.
≡ Passalora dendritica var. orbiculata (Desm.) Berk., in Saccardo,
Mycoth. Ven., Cent. XII, No. 1246. 1876 and Michelia 1(2): 265. 1878.
≡ Fusicladium dendriticum var. orbiculatum (Desm.) Sacc., Syll. fung. 4:
345. 1886.
= Venturia inaequalis (Cooke) G. Winter, Hedwigia 36: 81. 1897.
= Fusicladium pomi (Fr.) Lind, Dan. fung.: 521. 1913.
Lit.: Lindau (1907: 782), Oudemans (1921), Ritschel (2001), Crous
& Braun (2003: 485), Schubert et al. (2003: 76).
Notes: For further synonyms see Schubert et al. (2003).
orchidis E.A. Ellis & M.B. Ellis, Mycol. Pap. 131: 17. 1972.
Fig. 383.
Holotype: UK, Norfolk, Horsey Warren, on living leaves of
Dactylorhiza praetermissa (≡ Orchis praetermissa, Dactylorhiza
majalis subsp. praetermissa) (Orchidaceae), 17 Jul. 1955, E.A.
Ellis (IMI 60545).
≡ Fusicladium orchidis (E.A. Ellis & M.B. Ellis) K. Schub. & U. Braun,
Sydowia 56(2): 314. 2004.
Lit.: Ellis (1976: 338), Ellis & Ellis (1985).
Ill.: Ellis (1972: 18, ig. 17), Ellis (1976: 339, ig. 257 A), Schubert &
Braun (2004: 315, ig. 8).
osterici Ces., in herb.
Specimen: Italy, Brescia, on Peucedanum verticillare (= Ostericum verticillare)
(Apiaceae), 1846, Cesati (B 700006650, 700006651).
= Passalora depressa (Berk. & Broome) Sacc., Nuovo Giorn. Bot. Ital. 8: 187.
1876.
Fig. 383. Fusicladium orchidis (IMI 60545). Conidiophores and conidia. Scale bar
= 10 µm. K. Bensch del.
oudemansii Kupka, Oesterr. Bot. Z. 67: 157. 1918, nom. nov.
Type: Netherlands, on leaves of Phragmites australis (= P.
communis) (Poaceae).
≡ Cladosporium phragmitis J. Opiz ex Oudem., Ned. Kruidk. Arch., Ser. II,
6: 57. 1892, non C. phragmitis J. Opiz, 1852.
= Deightoniella arundinacea (Corda) S. Hughes, Mycol. Pap. 48: 29. 1952.
Lit.: Lindau (1907: 814), de Vries (1952: 96), David (1997: 137).
Notes: See C. phragmitis.
oxycocci Shear, Bull. Torrey Bot. Club 34(6): 306. 1907. Fig.
384.
Holotype: Canada, Nova Scotia, Arichat, on living leaves of
Vaccinium macrocarpon (Ericaceae), 21 Jun. 1902, C.L. Shear,
No. 1492 (BPI 427299).
≡ Stenella oxycocci (Shear) K. Schub. & U. Braun, Nova Hedwigia 84:
205. 2007.
≡ Zasmidium oxycocci (Shear) K. Schub. & U. Braun, Schlechtendalia
20: 102. 2010.
Lit.: Saccardo (1913a: 1368).
Ill.: Schubert & Braun (2007: 206, ig. 9).
paeoniae Pass., in Thümen, Herb. Mycol. Oecon., Fasc. IX,
No. 416. 1876 and Just’s Bot. Jahresber. 4: 235. 1876.
Lectotype (selected in Schubert et al., 2007a): Italy, on living
leaves of Paeonia lactilora (= P. edulis) (Paeoniaceae), Thümen,
Herb. Mycol. Oecon. 416 (M-0057753). Isolectotypes: Thümen,
Herb. Mycol. Oecon. 416 and Thümen, Mycoth. Univ. 670 (e.g., B
700006655, HAL, M-0057752).
= Periconia chlorocephala Fresen., Beiträge zur Mykologie 1: 21. 1850.
≡ Graphiopsis chlorocephala (Fresen.) Trail, Scott. Naturalist (Perth)
10: 75. 1889.
≡ Dichocladosporium chlorocephalum (Fresen.) K. Schub., U. Braun &
Crous, Stud. Mycol. 58: 96. 2007.
Lit.: Saccardo (1886: 362), Lindau (1907: 822), Ferraris (1912: 348),
Lind (1913: 524), de Vries (1952: 94), McKemy & Morgan-Jones
(1991a), Schubert et al. (2007a), Braun et al. (2008a: 208–209).
Notes: See C. chlorocephalum.
324
Fig. 384. Zasmidium oxycocci (BPI 427299, holotype of C. oxycocci). Conidiophores
and conidia. Scale bar = 10 µm. K. Bensch del.
paeoniae var. paeoniae-anomalae Sacc., Syll. fung. 4: 362.
1886.
Type: Russia, Siberia, on faded leaves of Paeonia anomala
(Paeoniaceae).
= Cladosporium paeoniae (“paeoniae-anomalae”) Sacc., Michelia 2(6): 148.
1880.
= Graphiopsis chlorocephala (Fresen.) Trail, Scott. Naturalist (Perth) 10: 75.
1889.
≡ Dichocladosporium chlorocephalum (Fresen.) K. Schub., U. Braun &
Crous, Stud. Mycol. 58: 96. 2007.
Lit.: Lindau (1907: 822), McKemy & Morgan-Jones (1991a),
Schubert et al. (2007a), Braun et al. (2008a: 208–209).
“paeoniae-anomalae” Sacc., Michelia 2(6): 148. 1880.
Notes: Saccardo (1880) refers to C. paeoniae reported on Paeonia
anomala.
the genuS Cladosporium
palmetto Ger. – an error in Vanev & Taseva (1990).
Notes: In “Index fungorum” only Helminthosporium palmetto
W.R. Gerard, Grevillea 17: 68, 1889, is listed. This species was
transferred to the genus Pleurophragmium by Hughes (1958) and
later to Spiropes by Ellis (1968).
parasiticum Sorokīn, Mikol. ocherki: 30. 1871. Fig. 385.
Type: Russia, Saratov, on abdomen of Melolontha fullonis
(Coleoptera) (location unknown).
Lit.: Saccardo (1906: 681, 1913a: 1370).
Notes: In Saccardo (1906) under Strumella parasitica C. Wize (l.c.),
[Pilze des Cleon. punctiventr. Crac. 1905: 725, ig. 11 (Bull. Acad.
Crac.)] “An Cladosporium parasiticum Sorok. (ubi?)”. Also cited in
Guéguen, Champ. paras. homme: 256 (1904). Excluded. Status
unclear.
paulliniae Deighton, Mycol. Pap. 144: 54. 1979. Fig. 386.
Holotype: Ghana, Essipun, on leaves of Paullinia pinnata
(Sapindaceae), 9 May 1949, S.J. Hughes (IMI 37238a).
≡ Stenella paulliniae (Deighton) K. Schub. & U. Braun, Mycol. Progr. 4(2):
107. 2005.
≡ Zasmidium paulliniae (Deighton) K. Schub. & U. Braun,
Schlechtendalia 20: 102. 2010.
Ill.: Deighton (1979: 54, ig. 28), Schubert & Braun (2005a: 107, ig. 7).
pelliculosum Berk. & M.A. Curtis, in herb.
Fig. 385. Fusicladium parasiticum (K 130656, neotype of C. phyllochorae).
Conidiophores and conidia. Scale bar = 10 µm. U. Braun del.
Specimen: USA, South Carolina, on leaves of Lobelia puberula (Campanulaceae),
No. 1742 (K 121567).
≡ Passalora lobeliae-cardinalis (Schwein.) U. Braun & Crous,
Mycosphaerella and its anamorphs: 1. Names published in Cercospora
and Passalora, CBS Biodiversity Ser. 1: 254. 2003.
Lit.: Cooke (1889), Saccardo (1892: 602, 1895: 621), Schubert &
Braun (2005b: 201–202).
Notes: Cooke (1889): “Scarcely appears to differ from Cladosporium
effusum Berk. & M.A. Curtis, and does not seem to have been
described”.
pericarpium Cooke, Grevillea 12(61): 31. 1883.
Syntypes: USA, South Carolina, Aiken, on husks of walnut (Juglans
nigra) (Juglandaceae), Ravenel, Fungi Amer. Exs. 597 (e.g., BPI
427378, K (F) 121566, NY, PH 01020429).
Lit.: Saccardo (1886: 353).
Notes: Excluded. Status unclear. Various hyphomycetes,
including a few conidia agreeing with Cooke’s original description
and conidiophore fragments found, but without any trace of
Cladosporium-like scars.
persicum (Sacc.) Tsuji, in herb.
Specimen: Japan, Iwate, Morioka, on Prunus persica (Rosaceae), 27 Oct. 1927, K.
Togashi (BPI 427388).
= Stenella persicae T. Yokoy. & Nasu, Mycoscience 41: 92. 2000.
Lit.: Schubert & Braun (2007: 206–207).
Notes: The collection examined agrees well with the species
concept of Stenella persicae, recently described on fruits of peach
from Japan (Yokoyama & Nasu 2000). The Japanese collection
was inscribed and deposited at BPI as “Cladosporium persicum
(Sacc.) Tsuji”, which is, however, a lapsus that has to be corrected
to Clasterosporium persicum (Sacc.) Tsuji, a species quite distinct
from Stenella persicae.
personatum Berk. & M.A. Curtis, Grevillea 3(27): 106. 1875.
Syntypes: USA, Santee River, on leaves of Arachis hypogaea
(Fabaceae), Ravenel, No. 1612 (IMI 104553, K).
www.studiesinmycology.org
Fig. 386. Zasmidium paulliniae (IMI 37238a, holotype of C. paulliniae). Conidiophores
and conidia. Scale bar = 10 µm. K. Bensch del.
≡ Cercospora personata (Berk. & M.A. Curtis) Ellis & Everh., J. Mycol.
1: 63. 1885.
≡ Cercosporiopsis personata (Berk. & M.A. Curtis) Miura, Flora of
Manchuria and East Mongolia, III. Cryptog. Fungi: 529. 1928, as
“personatum”.
325
BenSch et al.
≡ Passalora personata (Berk. & M.A. Curtis) S.A. Khan & M. Kamal,
Pakistan J. Sci. Res. 13: 188. 1961.
≡ Cercosporidium personatum (Berk. & M.A. Curtis) Deighton, Mycol.
Pap. 112: 71. 1967.
≡ Phaeoisariopsis personata (Berk. & M.A. Curtis) Arx, Proc. Kon. Ned.
Akad. Wetensch. C, 86(1): 43. 1983.
≡ Passalora personata (Berk. & M.A. Curtis) Poonam Srivast., J. Liv.
World 1(2): 117. 1994, comb. inval. et nom. illeg.
= Septogloeum arachidis Racib., Z. Planzenkrankh. 8: 66. 1898.
= Cercospora arachidis Henn., Hedwigia 41: 18. 1902. [Type: B].
= ?Mycosphaerella berkeleyi W.A. Jenkins, J. Agric. Res. 56: 330. 1938.
Lit.: Saccardo (1886: 439), Sivanesan (1984: 219), Crous & Braun
(2003: 317).
personatum f. arachidis-hypogaeae Thüm., Herb. Mycol.
Oecon., Fasc. XIII, No. 608. 1878, nom. nud.
Type: USA, South Carolina, on living leaves of Arachis hypogaea
(Fabaceae), Thümen, Herb. Mycol. Oecon. 608 (e.g. B 700006673).
Notes: No fructiication found on the type material, but this forma is
probably synonymous with Passalora personata.
personatum var. cassiae Thüm., Mycoth. Univ., Cent. XX, No.
1964. 1881.
Syntypes: USA, South Carolina, Aiken, on living leaves of Senna
occidentalis (≡ Cassia occidentalis) (Fabaceae), 1876, H.W. Ravenel,
Thümen, Mycoth. Univ. 1964 (e.g., BPI, G, HBG, HAL, S, W).
= Cercospora personata var. cassiae-occidentalis Berk. & M.A. Curtis,
Grevillea 3(27): 106. 1875. [Types: IMI 104555, K].
= Cercospora occidentalis Cooke, Hedwigia 17: 39. 1878. [Types: BPI
438975, K, IMI 92632a, Ellis & Everhart, N. Amer. Fungi 642, Ravenel, Fungi
Amer. Exs. 65].
≡ Ramularia cassiicola Heald & F.A. Wolf, U.S.D.A. Bur. Pl. Industr. Bull. 226:
101. 1912, nom. nov., non Ramularia occidentalis Ellis & Kellerm., 1883.
≡ Phaeoramularia occidentalis (Cooke) Deighton, More Dematiaceous
Hyphomycetes: 322. 1976.
≡ Passalora occidentalis (Cooke) U. Braun, Schlechtendalia 5: 70.
2000.
= Cercospora sphaeroidea Speg., Anales Soc. Ci. Argent. 9: 279. 1880.
[Types: IMI 206774, LPS 911].
≡ Phaeoisariopsis sphaeroidea (Speg.) L.G. Br. & Morgan-Jones,
Mycotaxon, 4: 303. 1976.
= Cercospora cassiicola Roum., Fungi Sel. Gall. Exs., No. 4486. 1886, as
“cassiaecola”, nom. nud. [lectotype (designated here): LEP].
= Cercospora paulensis Henn., Hedwigia 48: 18. 1909. [Type: B].
= Cercospora iponemensis Henn., Hedwigia 48: 18. 1909. [Type: B].
≡ Cercosporina iponemensis (Henn.) Sacc., Syll. fung. 25: 906. 1931.
= Cercospora occidentalis Ellis & Kellerm., U.S.D.A. Bur. Pl. Industr. Bull. 226:
101. 1912, nom. illeg., homonym, non C. occidentalis Cooke, 1878.
= Cercospora somalensis Curzi, Boll. Staz. Patol. Veg. Roma, N.S., 12: 158.
1932.
Lit.: Ellis (1976: 322), Crous et al. (2000), Crous & Braun (2003:
294).
pestis Thüm., Herb. Mycol. Oecon., Fasc. IX, No. 419. 1876
and Oesterr. Bot. Z. 27: 12. 1877.
Syntype: Austria, Klosterneuburg, Krems, on living leaves of Vitis
vinifera (Vitaceae), 1876, von Thümen, Thümen, Herb. Mycol.
Oecon. 419 (e.g., M).
= Passalora dissiliens (Duby) U. Braun & Crous, Mycosphaerella and
its anamorphs: 1. Names published in Cercospora and Passalora, CBS
Biodiversity Ser. 1: 164. 2003.
Lit.: Saccardo (1886: 458), Lindau (1910: 117), Crous & Braun
(2003: 164).
Notes: See also C. roesleri.
phaseoli Fuss, Archiv Vereins Siebenb. Landesk., N.F., 14(2):
431. 1878, nom. nud.
Specimen: Romania, Giresau, on overwintered fruits of Phaseolus vulgaris
(Fabaceae), Fuss (location unknown).
326
Notes: Classiied by Fuss (1878) as a forma of C. herbarum.
phragmitis J. Opiz, Seznam: 117. 1852, nom. nud., non C.
phragmitis J. Opiz ex Oudem., 1892.
Type: Czech Republic, on leaves of Phragmites australis (= P.
communis) (Poaceae) (PRM).
= Heterosporium phragmitis Sacc., Rev. Mycol. (Toulouse) 6: 37. 1884. as
“(Opiz?) Sacc.”.
Lit.: Saccardo (1886: 370), Lindau (1907: 814), Oudemans (1919),
de Vries (1952: 96).
Notes: Excluded. This species represents a “Fumago” (Kupka
1918, David 1997).
phragmitis J. Opiz ex Oudem., Ned. Kruidk. Arch., Ser. 2,
6(1): 57. 1892.
Type: Netherlands, Bien de Campagne Zorgvlied, near Haye, on
leaves of Phragmites australis (= P. communis) (Poaceae), Jul.
1889, C.E. Destrée.
≡ Cladosporium oudemansii Kupka, Oesterr. Bot. Z. 67: 157. 1918.
= Helminthosporium arundinaceum Corda, Icon. fung. 3: 10. 1839, as
“Helmisporium”.
≡ Napicladium arundinaceum (Corda) Sacc., Syll. fung. 4: 482. 1886.
≡ Deightoniella arundinacea (Corda) S. Hughes, Mycol. Pap. 48: 29.
1952.
Lit.: de Vries (1952: 96), David (1997: 137).
Notes: David (1997): “Oudemans (1892) provided a description of
what he took to be Opiz’s species. This description, also given by
Lindau (1907), is of an entirely different fungus, as noted by Kupka
(1918) and de Vries (1952)”.
phyllachorae M.B. Ellis, More Dematiaceous Hyphomycetes:
332. 1976.
Neotype (selected in Schubert & Braun, 2007): Philippines,
Prov. Laguna, Luzon, on Phyllachora pseudes (Phyllachoraceae)
on dead leaves of Ficus nota (Moraceae), Oct. 1915, M. Ramos,
Bureau of Sciences No. 23781 [K (M) 130656].
≡ Monotospora parasitica Syd. & P. Syd., Ann. Mycol. 15: 263. 1917, non
Cladosporium parasiticum Sorokin, 1891.
≡ Fusicladium parasiticum (Syd. & P. Syd.) U. Braun & K. Schub., Nova
Hedwigia 84: 193. 2007.
Lit.: Heuchert et al. (2005: 56–57).
Ill.: Ellis (1976: 333, ig. 251), Schubert & Braun (2007: 194, ig. 3).
Notes: A further collection for Monotospora parasitica is mentioned
in the original diagnosis on Phyllachora pseudes on leaves of Ficus
nota, Phillipines, Luzon, Prov. Laguna, San Antonio, Oct. 1915, M.
Ramos (Bureau of Sciences 23781).
piricularioides Dearn. & House, Circ. New York State Mus.
24: 57. 1940, nom. inval.
Syntypes: USA, New York, Essex Co., Newcomb, on leaves of
Panicum boreale (Poaceae), 17 Aug. 1924, H.D. House (DAOM
5741, NY, NYS 2365).
= Passalora fusimaculans var. barretoana U. Braun & Crous, Mycosphaerella
and its anamorphs: 1. Names published in Cercospora and Passalora. CBS
Biodiversity Ser. 1: 453. 2003.
≡ Passalora barretoana (U. Braun & Crous) D.J. Soares, U. Braun &
R.W. Barreto, Australas. Pl. Pathol. 35(3): 348. 2006.
Lit.: Schubert & Braun (2005a: 104–105).
pithecolobii Kulhara & Singh, in herb. (?)
Specimen: India, M. Pradesh, Jabalpur, on Pithecellobium dulce (Fabaceae).
polymorphosporum R.F. Castañeda & W.B. Kendr., Univ.
Waterloo Biol. Ser. 35: 24. 1991.
the genuS Cladosporium
Holotype: Cuba, Pinar del Río, Sandino, on stem of an unidentiied
grass, 24 Mar. 1990, R.F. Castañeda (INIFAT C90/139).
Ill.: Castañeda & Kendrick (1991: 23, ig. 12).
Notes: Excluded, not Cladosporium s. str., but status not yet clear.
polymorphum Peyl, Lotos 15: 18. 1865 and Hedwigia 5: 60.
1866.
Type: Czech Republic, Kačina, near Neuhof, on fruits of Pyrus sp.
(Rosaceae), 1864.
= Helminthosporium pyrorum Lib. (p.p.), Pl. Crypt. Arduenna, Fasc. 2, No. 188.
1832. [Type: e.g., DAOM].
≡ Fusicladium pyrorum (Lib.) Fuckel, Jahrb. Nassauischen Vereins
Naturk. 23–24: 357. “1869”, 1870, as “Fusicladium pyrinum”.
≡ Passalora pyrina (Lib.) Sacc., Michelia 1: 537. 1879.
≡ Megacladosporium pyrorum (Lib.) Vienn.-Bourg., Les Champignons
parasites des plantes cultivèes 1: 489. 1949, as “Megacladosporium
pirinum”.
= Arthrinium pyrinum Wallr., Fl. crypt. Germ. 2: 163. 1833. [Types: IMI 68300,
STR].
= Fusidium pyrinum Corda, Icon. Fung. 1: 3. 1837. [Type: PRM].
= Fusicladium virescens Bonord., Handb. Mykol.: 80. 1851. [Iconotype:
Bonorden, 1851: Fig. 94].
= Fusicladium fuscescens Rabenh., Bot. Zeitung (Berlin) 15: 430. 1857.
[Syntypes: Rabenhorst, Herb. Viv. Mycol. 588, e.g., HAL, HBG].
= Passalora pomi G.H. Otth, Mitteil. Naturf. Ges. Bern 1868: 66. 1868. [Type:
BERN].
= Fusicladium pyrorum var. cladophilum Ellis & Everhart, N. Amer. Fungi, No.
2791. 1892. [Types: Ellis & Everhart, N. Amer. Fungi 2791, e.g., BPI, M, NY].
= Venturia pyrina Aderh., Landw. Jahrb. 25: 875. 1896, as “pirina”.
= Cercospora porrigo Speg., Anales Mus. Nac. Buenos Aires, Ser. 2, 6: 341.
1899. [Holotype: LPS 934].
= Fusicladium pyrorum f. carpophila Sacc., Mycoth. Ital., No. 992. 1901.
[Types: Saccardo, Mycoth. Ital. 992, e.g., B].
= Acrotheca dearnessiana Sacc., Ann. Mycol. 10: 314. 1912. [Types:
Bartholomew, Fungi Columb. 5001, e.g., IMI 7073].
≡ Fusicladium dearnessianum (Sacc.) M.B. Ellis, in herb.
Fig. 387. Fusicladium psammicola (PAD, holotype of Exosporium psammicola).
A. Hyphae, hyphal aggregations and strands. B. Fasciculate conidiophores. C.
Conidiogenous cells. D. Conidia. Scale bar = 10 µm. U. Braun del.
Ammophila arenaria (≡ Psamma arenaria) (Poaceae), 18 May
1913, A. Trotter (PAD).
Basionym: Exosporium psammicola Sacc., Ann. Mycol. 11: 420.
1913.
≡ Fusicladium psammicola (Sacc.) U. Braun & K. Schub., Mycotaxon
103: 212. 2008.
Lit.: Lindau (1907: 781), Oudemans (1921), Schubert et al. (2003:
82).
Ill.: Morgan-Jones & Kendrick (1972: 1818, ig. 1), Braun et al.
(2008a: 213, ig. 3).
polysporum Link, Sp. pl. 6(1): 40. 1824.
puccinioides Cooke, Grevillea 5(33): 15. 1876. Fig. 388.
Type: Germany, Berlin, on rotten wood, Link (B 700006685).
= Trichoderma globosum Schwein., Syn. fung. Carol. sup.: 77. 1822 : Fr., Syst.
Mycol. 3(1): 215. 1829. [Syntypes: BPI, K, PH, UPS].
≡ Oidium inquinans Schwein., Trans. Amer. Philos. Soc., N.S., 4(2): 286.
1832, nom. nov.
≡ Torula inquinans (Schwein.) Sacc., Syll. fung. 4: 251. 1886.
≡ Streptothrix globosa (Schwein. : Fr.) S. Hughes, Canad. J. Bot. 31: 606.
1953.
≡ Conoplea globosa (Schwein. : Fr.) S. Hughes, Canad. J. Bot. 36: 755.
1958.
= Steptothrix atra Berk. & M.A. Curtis, Grevillea 3(27): 107. 1875.
= Strumella coryneoidea Sacc. & G. Winter, in Rabenhorst, Fungi Eur. Exs.,
Cent. XXX, No. 2984. 1883 and Hedwigia 22(11): 175. 1883. [Syntypes: e.g.,
CUP, HAL, HBG, M].
= Trichosporium densum P. Karst., Hedwigia 23(4): 59. 1884.
= Streptothrix pereffusa Sumst., Mycologia 6: 34. 1914.
Lit.: Saccardo (1886: 354), Lindau (1907: 831), de Vries (1952: 96).
porophorum Matsush., Icones Microfungorum a Matsushima
Lectorum: 36. 1975.
Holotype: Japan, isol. from seeds of Raphanus sativus
(Brassicaceae), Feb. 1969 (Matsush. herb. 2578).
= Chalastospora gossypii (Jacz.) U. Braun & Crous, Persoonia 22: 144.
(2009).
Lit.: Ho et al. (1999: 134).
Notes: See C. malorum Ruehle.
psammicola (Sacc.) Morgan-Jones & W.B. Kendr., Canad. J.
Bot. 50(9): 1817. 1972. Fig. 387.
Holotype: Libya, Ras Carrac in Magna Syrte, on dead leaves of
www.studiesinmycology.org
Holotype: India, on under side of living leaves of an unidentiied
host plant, 1876, Colonel Hobsen, No. 57 (K 121568).
≡ Prathigada puccinoides (Cooke) M.B. Ellis, in herb.
≡ Pseudoasperisporium puccinioides (Cooke) K. Schub. & U. Braun,
Fungal Diversity 20: 202. 2005.
Lit.: Saccardo (1886: 361), Subramanian (1971: 291).
Ill.: Cooke (1876: pl. 74, ig. 11), Schubert & Braun (2005b: 203, ig. 6).
pulcherrimum Ellis & Everh., N. Amer. Fungi, Ser. 2, Cent.
XXIX, No. 2877. 1893, nom. nud.
Type: USA, Ontario, on Carpinus sp. (Betulaceae), Ellis & Everhart,
N. Amer. Fungi 2877 (e.g., NY).
Lit.: Cash (1952: 69).
pullulans (de Bary) Sacc., Syll. fung. 22: 1250. 1913.
Neotype: France, on fruits of Vitis vinifera (Vitaceae). Ex-neotype
culture: CBS 584.75.
≡ Dematium pullans de Bary, Vergl. Morph. Biol. Pilze: 182. 1884.
≡ Oidium pullulans (de Bary) Lindner, Wochenschr. Brauerei 15: 209–
213. 1898.
≡ Oospora pullulans (de Bary) Sacc., Syll. fung. 18: 499. 1906.
≡ Aureobasidium pullulans (de Bary) G. Arnaud (var. pullulans), Ann.
Écol. Nat. Agric. Montpellier, N.S., 16: 39. 1918.
≡ Pullularia pullulans (de Bary) Berkhout, Die schimmelgeschlachten
Monilia, Oidium, Oospora en Torula: 55. 1923.
≡ Hormonema pullulans (de Bary) Lagerb. & Melin, Nyt Mag. Naturvidensk
71: 256. 1932.
Notes: Cited by Saccardo (1913) under Oidium erysiphoides Fr. f.
cordiae Sacc., Ann. Mycol. 8: 339 (1910): “Hab. In foliis Cordiae
327
BenSch et al.
Fig. 389. Zasmidium quitense (B 700006694, lectotype of C. quitense).
Conidiophores and conidia. Scale bar = 10 µm. K. Bensch del.
Fig. 388. Pseudoasperisporium puccinioides (K 121568, holotype of C.
puccinioides). Fascicle of conidiophores, conidiogenous cells and conidia. Scale
bar = 10 µm. K. Bensch del.
suboppositae … Socium adest Cladosporium (Demat.) pullulans.”.
In Saccardo (1886), Lindau (1907) and Ferraris (1912) “Dematium
pullulans de Bary & Löwenthal” is cited as synonym of Cladosporium
herbarum. For further synonyms and comments on Aureobasidium
pullulans see Subramanian (1971), de Hoog & Yurlova (1994) and
Yurlova et al. (1999).
punctiforme Fuckel, Fungi Rhen. Exs., Fasc. II, No. 116.
1863.
Syntype: Germany, “auf der Geis im Hattenheimer Wald”, on living
leaves of Sanicula europaea (Apiaceae), Fuckel, Fungi Rhen. Exs.
116 (e.g., HAL).
≡ Cercospora punctiformis (Fuckel) G.A. de Vries, Contr. Knowl. Genus
Cladosporium: 97. 1952, nom. illeg, homonym, non C. punctiformis Sacc.
& Roum., 1881.
= Cercospora saniculae-europaeae E. Müll. & Arx, Phytopathol. Z. 24: 356.
1955.
≡ Pseudocercospora saniculae-europaeae (E. Müll. & Arx) U. Braun
& Crous, Mycosphaerella and its anamorphs: 1. Names published in
Cercospora and Passalora, CBS Biodiversity Ser. 1: 365. 2003.
Lit.: Fuckel (1870: 355), Saccardo (1886: 362), Lindau (1907: 825),
Oudemans (1923).
putrefaciens – Z. Planzenkrankh. 4: 333. 1894.
Notes: Without author on Beta sp. – maybe an error and
Clasterosporium putrefaciens (Fuckel) Sacc. was intended.
pygmaeum Ellis & Everh. (in Exs.: Flora Sequoia Gigantea
Region, No. 1235, nom. nud.).
Syntypes: USA, California, Amador Co., Pine Grove, on Vitis
californica (Vitaceae), Jul. 1893, G.E. Hansen (B 700006691–
700006692, BPI 427408–427409, NY).
= Asperisporium minutulum (Sacc.) Deighton, in Ellis, More Dematiaceous
Hyphomycetes: 242. 1976.
Lit.: Schubert & Braun (2005b: 188).
“pyrorum Berk.”, Gard. Chron. 1848: 398. 1848.
Lit.: Lindau (1907: 779), Oudemans (1921).
Notes: Lindau cited “Gard. Chron. p. 398, 1848” for C. pirorum Berk.
and listed it as synonym of Fusicladium dendriticum [= Fusicladium
pomi]. The page concerned has been examined, but the name
“Cladosporium pyrorum Berk.” was not found.
quitense Syd., Ann. Mycol. 37: 420. 1939. Fig. 389.
Lectotype: Ecuador, Pichincha mountains near Quito, on leaves
of Berberis schwerinii (Berberidaceae), 11 Sep. 1937, H. Sydow (B
700006694). Isolectotypes: B 700006693 and Sydow, Fungi Exot.
Exs. 1232, e.g., B 700006695, BPI 427427, M-0057728.
≡ Stenella quitensis (Syd.) K. Schub. & U. Braun, Mycol. Progr. 4(2): 108.
2005.
≡ Zasmidium quitense (Syd.) K. Schub. & U. Braun, Schlechtendalia
20: 103. 2010.
Ill.: Schubert & Braun (2005a: 108, ig. 8).
radians Sacc. & D. Sacc., Syll. fung. 16: 1059. 1902. Fig.
390.
Lectotype: Italy, Padua, on leaves of Abies pinsapo (Pinaceae), Apr.
1900, Saccardo, Mycoth. Ital. 787 (B 700006696). Isolectotypes:
328
the genuS Cladosporium
≡ Hormodendrum resinae Lindau, in Rabenhorst, Krypt.-Fl., ed. 2, 1(8):
699. 1907, as “Hormodendron”.
≡ Hormoconis resinae (Lindau) Arx & G.A. de Vries, Verh. Kon. Ned.
Akad. Wetensch., Afd. Natuurk., Tweede Sect., 61(4): 62. 1973.
= Racodium resinae Fr., Observ. mycol. 1: 216. 1815. [Type: B, DAOM 41890
(slide)].
≡ Sporocybe resinae (Fr.) Fr., Syst. mycol. 3(2): 341. 1832.
≡ Sorocybe resinae (Fr.) Fr., Summa veg. Scand. 2: 468. 1849
(synnematous form, H. resinae is the mononematous form of this species).
≡ Dendryphion resinae (Fr.) Corda, Icon. fung. 6: 11. 1854.
≡ Stysanopsis resinae (Fr.) Ferraris, Flora Ital. Crypt., Pars I, Fungi, Fasc.
6: 187. 1910.
= Pycnostysanus resinae Lindau, Verh. Bot. Vereins Prov. Brandenburg 45:
160. 1904. [Type: B].
≡ Stysanus resinae (Lindau) Sacc., Syll. fung. 18: 651. 1906.
Fig. 390. Septonema acicola (B 700006696, lectotype of C. radians). Conidiophores
and conidia. Scale bar = 10 µm. K. Bensch del.
Lit.: Ellis (1971: 309), Ho et al. (1999: 149), Partridge et al. (2001:
179), Partridge & Morgan-Jones (2002: 344–348), Seifert et al.
(2007).
Notes: Based on the type material, the name Hormodendrum resinae
refers to the mononematous state of the resin fungus. However, this
name was often confused with and applied for the anamorph of
Amorphotheca resinae, the creosote fungus. The oldest valid name
for the latter species is, however, Cladosporium avellaneum.
Saccardo, Mycoth. Ital. 787, e.g., B 700006696, BPI 427428, HAL,
HBG, SIENA.
resinae f. albidum (G.A. de Vries) G.A. de Vries, Antonie van
Leeuwenhoek J. Microbiol. Serol. 21: 167. 1955.
≡ Cladosporium radians Sacc. & D. Sacc., Mycoth. Ital., Cent. VIII., No.
787. 1901, nom. nud.
≡ Septonema acicola U. Braun & K. Schub., Nova Hedwigia 84: 200.
2007.
Lit.: Lindau (1907: 812), Ferraris (1912: 336).
Ill.: Schubert & Braun (2007: 201, ig. 6).
ramulosum Rab. – Oudemans (1924).
Notes: An error. C. ramulosum Roberge ex Desm. was intended.
ramulosum Roberge ex Desm., Ann. Sci. Nat. Bot., Sér. 3,
18: 361. 1852, nom. illeg., non C. ramulosum Reissek, 1851.
Holotype: France, Paris, Parc du Libisy, on Populus alba
(Salicaceae), May 1851, Roberge (PC 1518). Isotype: herb.
Desmazières 2135 (PC).
≡ Fusicladium ramulosum Rostr., Tidsskr. Skovbr. 6: 294. 1883, nom.
nov., as “(Roberge, in Desm.) Rostr.”.
≡ Pollaccia ramulosa (Rostr.) Ondřej, Eur. J. Forest Pathol. 2: 143. 1972,
nom. nov., as “(Desm.) Ondřej”.
= Fusicladium radiosum (Lib.) Lind, Ann. Mycol. 3: 429. 1905 (var. radiosum).
Lit.: Saccardo (1886: 357), Lindau (1907: 777), Oudemans (1920),
Baldacci & Ciferri (1937: 61), Ritschel (2001), Schubert et al. (2003:
85).
Notes: See C. asteroma.
rectum Preuss, in Sturm, Deutschl. Fl. 3(26): 29. 1848.
Holotype: Germany, near Hoyerswerda, on the innerside of bark of
Pinus (Pinaceae), Preuss (B 700006697).
= Helminthosporium fasciculare Corda, Icon. fung. 1: 14. 1837, as
“Helmisporium”. [Type: PRM].
≡ Septonema fasciculare (Corda) S. Hughes, Canad. J. Bot. 36: 803.
1958.
= Dendryphion pini Höhn., Sitzungsber. Kaiserl. Akad. Wiss., Math.-Naturwiss.
Cl., Abt. 1, 116: 153. 1907. [Type: FH].
Lit.: Saccardo (1886: 354, 374, 1906: 577), Lindau (1907: 810).
resinae (Lindau) G.A. de Vries, Antonie van Leeuwenhoek J.
Microbiol. Serol. 21: 167. 1955.
Holotype: Germany, Fl. v. Hamburg, Sachsenwald, on resin of
Picea abies (Pinaceae), 29 Apr. 1904, O. Jaap (B). Isotype: DAOM
41888 (slide).
www.studiesinmycology.org
Basionym: Cladosporium avellaneum f. albidum G.A. de Vries,
Contr. Knowl. Genus Cladosporium: 56. 1952.
Notes: See C. avellaneum f. albidum.
resinae f. avellaneum (G.A. de Vries) G.A. de Vries, Antonie
van Leeuwenhoek J. Microbiol. Serol. 21: 167. 1955.
Basionym: Cladosporium avellaneum f. avellaneum G.A. de Vries,
Contr. Knowl. Genus Cladosporium: 56. 1952.
Notes: See C. avellaneum.
resinae f. sterile (G.A. de Vries) G.A. de Vries, Antonie van
Leeuwenhoek J. Microbiol. Serol. 21: 167. 1955.
Basionym: Cladosporium avellaneum f. sterile G.A. de Vries, Contr.
Knowl. Genus Cladosporium: 56. 1952.
Notes: See C. avellaneum f. sterilis.
resinae f. viride G.A. de Vries) G.A. de Vries, Antonie van
Leeuwenhoek J. Microbiol. Serol. 21: 167. 1955.
Basionym: Cladosporium avellaneum f. viride G.A. de Vries, Contr.
Knowl. Genus Cladosporium: 56. 1952.
Notes: See C. avellaneum f. viride.
rhododendri, in herb.
Specimen: Switzerland, Bern, Berner Oberland, vom Faulhorn, on leaves of
Rhododendron sp. (Ericaceae), 7/58 (B 700006700).
Notes: Excluded, generic afinity not yet clear.
rhodomyrti Sawada, Rep. Gov. Res. Inst. Formosa 87: 74.
1944, nom. inval.
Holotype: Taiwan, on Rhodomyrtus tomentosa (Myrtaceae)
(PPMH).
Notes: Description only in Japanese, not validly published.
Excluded, generic afinity remains unclear.
rhois Arcang., in Thümen, Mycoth. Univ., Cent. XIV, No.
1371. 1879.
Syntypes: Italy, Etruria, Tuscany, Settignano, near Florence,
on living leaves of Rhus coriaria (Anacardiaceae), Nov. 1878,
329
BenSch et al.
Arcangeli, Thümen, Mycoth. Univ. 1371 and Baglietto et al., Erb.
Critt. Ital. 849 (e.g., BPI 427440, E, HAL, K).
= Cercospora marmorata Tranzschel, Mycoth. Ross., Fasc. 5, No. 250. 1911.
[Types: e.g., K, LE, W].
≡ Cercosporina marmorata (Tranzschel) Sacc., Syll. fung. 25: 895. 1931.
≡ Phaeoramularia marmorata (Tranzschel) Deighton, Mycol. Pap. 144:
34. 1979.
≡ Passalora marmorata (Tranzschel) U. Braun & Crous, Mycosphaerella
and its anamorphs: 1. Names published in Cercospora and Passalora,
CBS Biodiversity Ser. 1: 267. 2003.
= Cercospora rhois-coriariae Kuhnh.-Lord., Ann. Épiphyt., Ser. 2, 13: 54. 1947.
Lit.: Saccardo (1886: 359), Lindau (1907: 827), Ferraris (1912:
346).
rietmanni Sart. & Syd., Rev. Pat. Malad. Pays Chauds 15(1):
9–44. 1935.
Type: Isol. from man (mycosis of the epidermis) (location unknown).
= Hortaea werneckii (Horta) Nishim. & Miyaji, Jap. J. Med. Mycol. 26(2): 145.
1984.
Lit.: Ciferri (1960: 501), de Hoog et al. (2000: 721).
Notes: See C. werneckii.
rigidiphorum R.F. Castañeda, in herb.
Holotype: Cuba, on dead leaves of Smilax sp. (Smilacaceae), 6
Nov. 1994, R.F. Castañeda (CBS H-19938). Ex-type culture: CBS
314.95, MUCL 39142.
≡ Penidiella rigidophora Crous, R.F. Castañeda & U. Braun, Stud.
Mycol. 58: 21. 2007.
Ill.: Crous et al. (2007b: 22–23, igs 12–13).
roesleri Catt., Bol. Commiss. Agrar. Voghera 13: 263. 1876.
Type: France, Dep. de l’Eure, Eburense, on Vitis vinifera (Vitaceae),
A. Malbranche.
≡ Cercospora roesleri (Catt.) Sacc., Michelia 2(6): 128. 1880.
≡ Cercospora roesleri “f. typica (Catt.)” Elenkin, Bolezni Rast. 4: 67. 1909,
nom. inval.
≡ Ragnhildiana roesleri (Catt.) Vassiljevsky, Parazitnye nesovershennye
griby, Ch. I, Gifomitsety: 375. 1937.
= Torula dissiliens Duby, Mem. Soc. Phys. Genève 7: 128. 1835.
≡ Septocylindrium dissiliens (Duby) Sacc., Mycoth. Ven., No. 583. 1876.
≡ Phaeoramularia dissiliens (Duby) Deighton, More Dematiaceous
Hyphomycetes: 324. 1976.
≡ Passalora dissiliens (Duby) U. Braun & Crous, Mycosphaerella and
its anamorphs: 1. Names published in Cercospora and Passalora, CBS
Biodiversity Ser. 1: 164. 2003.
= ? Septocylindrium virens Sacc., Nuovo Giorn. Bot. Ital. 8: 186. 1876.
= Septosporium fuckelii Thüm., Oesterr. Bot. Z. 27: 137. 1877.
≡ Cercospora fuckelii (Thüm.) Jacz., Parasitic fungal diseases of grape
vine, Ed. 2: 81. 1906.
≡ Cercospora roesleri f. fuckelii (Thüm.) Elenkin, Bolezni Rast. 4: 68.
1909.
≡ Isariopsis fuckelii (Thüm.) du Plessis, Farming South Africa 17: 62.
1942.
= Cladosporium pestis Thüm., Oesterr. Bot. Z. 27: 12. 1877.
= ? Cercospora coryneoides Săvul. & Rayss, Rev. Pathol. Vég. Entomol.
Agric. France 22: 223. 1935.
= Cercospora leoni Săvul. & Rayss, Rev. Pathol. Vég. Entomol. Agric. France
22: 222. 1935. [Type: HUJ].
= Cercospora judaica Rayss, Palestine J. Bot., Jerusalem Ser. III, 50: 22.
1943. [Type: HUJ].
Lit.: Saccardo (1886: 458, as “rosleri”), Lindau (1910: 117, as
“Rösleri”), Chupp (1954: 604), Crous & Braun (2003: 164).
salicis Moesz & Smarods, Magyar Bot. Lapok 31: 42. 1932.
Fig. 391.
Type: Latvia, near Adaži, on branches of Salix cinerea (Salicaceae),
10 Jun. 1930, J. Smarods (M-0057719). Topotypes: Behr, Plantae
rarae et novae 134-1939 (e.g., HAL), material collected at the type
locality in 1937.
330
Fig. 391. Fusicladium salicis (HAL, topotype of C. salicis). A. Conidiophores. B.
Conidia. Scale bar = 10 µm. U. Braun del.
Authentic material: Petrak, Mycoth. Gen. 1808 (e.g., BPI 427452,
M-0057720).
≡ Fusicladium salicis (Moesz & Smarods) U. Braun & K. Schub.,
Schlechtendalia 16: 73. 2007.
Lit.: Saccardo (1972: 1339).
Ill.: Moesz (1932: 43, ig. 6), Braun & Schubert (2007: 74, ig. 7).
salicis-sitchensis Dearn. & Barthol., Mycologia 16: 174. 1924.
Lectotype: USA, Washington, Langley, on living leaves of Salix
sitchensis (Salicaceae), Sep. 1922, Grant, No. 5011 (DAOM).
= Ramulaspera salicina var. tirolense Bubák & Kabát, Oesterr. Bot. Z. 55: 243.
1905.
≡ Ramularia salicina var. tirolense (Bubák & Kabát) Deighton, Trans. Brit.
Mycol. Soc. 90(2): 330. 1988.
≡ Phacellium salicinum var. tirolense (Bubák & Kabát) U. Braun, Nova
Hedwigia 56: 438. 1993.
Lit.: Saccardo (1972: 1339), Braun (1998: 337).
sambuci Pass., in herb.
Specimen: Italy, on living leaves of Sambucus nigra (Adoxaceae) (B 700006710).
= Pseudocercospora sambucigena U. Braun, Crous & K. Schub.,
Mycotaxon 92: 400. 2005.
Notes: The herbarium name Cladosporium sambuci proved to
be a true and new species of the genus Pseudocercospora. The
abundantly sporulating collection was sparsely intermixed with a
Cladosporium sp.
“sarcopodioides Sacc.” – Oudemans (1924).
Notes: An error. Clasterosporium sarcopodioides was intended.
scillae Deighton, New Zealand J. Bot. 8(1): 55. 1970.
Holotype: New Zealand, Levin, on living leaves of Scilla
peruviana (Asparagaceae), 21 Dec. 1965, G.F. Laundon, LEV
the genuS Cladosporium
477 (IMI 116997). Epitype: CBS H-19903. Ex-epitype culture:
CBS 116461.
≡ Fusicladium scillae (Deighton) U. Braun & K. Schub., IMI Descriptions
of Fungi and Bacteria 152, No. 1518. 2002.
≡ Cladophialophora scillae (Deighton) Crous, U. Braun & K. Schub.,
Stud. Mycol. 58: 198. 2007.
Lit.: Schubert et al. (2003: 94–96), Crous et al. (2007d: 198–199).
Ill.: Laundon (1970: 57, ig. 4), Braun & Schubert (2002: igs A–D),
Schubert et al. (2003: 95, ig. 47), Crous et al. (2007d: 199, ig. 12)
sclerotiophilum Sawada, Rep. Gov. Res. Inst. Formosa 51:
112. 1931, nom. inval.
Holotype: Taiwan, on twigs of Citrus maxima (= C. grandis f.
butan) (Rutaceae), 25 Nov. 1928, K. Sawada (PPMH).
Notes: Description only in Japanese. Excluded, but taxonomic
status not yet clear.
scopiforme Berk., Hooker’s J. Bot. Kew Gard. Misc. 6: 208.
1854, as “scopæforme”.
Type: India, Khasia (Churra), on leaves of Myristica sp.
(Myristicaceae), Hooker (K 115206, UPS).
≡ Helminthosporium scopiforme (Berk.) Subram., J. Indian Bot. Soc. 35:
450. 1956, as “scopæforme”.
≡ Pleurophragmium scopiforme (Berk.) S. Hughes, Canad. J. Bot. 36:
798. 1958, as “scopæforme”.
≡ Spiropes scopiformis (Berk.) M.B. Ellis, Mycol. Pap. 114: 30. 1968.
= Cladosporium congestum Berk. & Broome, J. Linn. Soc., Bot. 14: 99. 1873,
1875. [Type: K].
= Helminthosporium iteodaphnes Thüm., Rev. Mycol. (Toulouse) 2: 38. 1880.
[Type: W 89009].
≡ Cercospora iteodaphnes (Thüm.) Sacc., Syll. fung. 4: 464. 1886.
(IACM).
≡ Mycovellosiella solanicola (Viégas) Munt.-Cvetk., Lilloa 30: 178. 1960.
= Cercospora brachycarpa Syd., Ann. Mycol. 28: 207. 1930. [Type: IMI 8500a].
≡ Mycovellosiella brachycarpa (Syd.) Deighton, Mycol. Pap. 137: 8. 1974.
≡ Passalora brachycarpa (Syd.) U. Braun & Crous, Mycosphaerella and
its anamorphs: 1. Names published in Cercospora and Passalora, CBS
Biodiversity Ser. 1: 87. 2003.
= Cercospora jaguarensis Chupp & A.S. Mull., Bol. Soc. Venez. Ci. Nat. 8: 48.
1942, nom. inval. [Types: CUP, IMI 105210a].
Notes: Zhang et al. (1998c) mentioned a irst record of this species
from China on Solanum melongena.
sphaeroideum Cooke, Grevillea 8(46): 60. 1879.
Holotype: New Zealand, Canterbury Alps, on leaves of Poa foliosa
(Poaceae), No. 398 (K 121569).
= Passalora graminis (Fuckel) Höhn., Zentralbl. Bakteriol. Parasitenk., Abt.
2, 60: 6. 1923.
Lit.: Saccardo (1886: 365), Lind (1913), Schubert & Braun (2005b:
198–200).
sphaerosporum (sic) – Barron (1968: 130, ig. 55).
Notes: Neither C. sphaerospermum nor C. “sphaerosporum” are
amongst the Cladosporium species indexed by Barron (1968),
but Figure 55, of which a detail provides the illustration for the
cover and an enlargement the frontispiece for Barron (1968), is a
commendable illustration of typical C. sphaerospermum.
sphondylii Fuss, Archiv Vereins Siebenb. Landesk., N.F.,
14(2): 431. 1878, nom. nud.
Lit.: Saccardo (1886: 358).
Type: Romania, Grosscheuern, Giresau, on dry stems of
Heracleum sphondylium (Apiaceae), Fuss (location unknown).
Notes: Classiied by Fuss (1878) as a forma of C. herbarum.
scribnerianum Cavara, in Briosi & Cavara, Fung. Paras.
Piante Colt. Utili Ess., Fasc. 7/8, No. 187. 1892 and Hedwigia
31: 143. 1892.
staurophorum (W.B. Kendr.) M.B. Ellis, More Dematiaceous
Hyphomycetes: 333. 1976.
Syntype: Italy, Pavia, on leaves of Betula populifolia (Betulaceae),
1890, F.L. Scribner, Briosi & Cavara, Fung. Paras. Piante Colt. Utili
Ess. 187 (e.g., HAL).
Lectotype: UK, Cheshire, Delamere Forest, on fallen needles of
Pinus sylvestris (Pinaceae), 1957, isol. B. Kendrick (IMI 71590).
Lit.: Saccardo (1895: 620), Lindau (1907: 819), Ferraris (1912:
340), Schubert et al. (2003: 96–97).
Ill.: Schubert et al. (2003: 97, ig. 48).
Lit.: Ellis & Ellis (1985: 175), Ho et al. (1999: 140).
Ill.: Kendrick (1961: 833–834, igs 1–2; pl. 1, igs 3–5), Ellis (1976:
334, ig. 252 B), Ho et al. (1999: 141, igs 44–45), Seifert et al.
(2004: 920, igs 2–12).
≡ Fusicladium scribnerianum (Cavara) M.B. Ellis, More Dematiaceous
Hyphomycetes: 238. 1976.
sericeum Ellis & Everh., in herb.
Specimen: USA, West Virginia, on a decorticated limb of Magnolia fraseri
(Magnoliaceae), 18 Sep. 1895, L.W. Nutall (BPI 427456, BPI 427457).
Notes: Not Cladosporium, status unclear.
sidae Cif. & Gonz. Frag., Bol. Real Soc. Esp. Hist. Nat. 25:
455. 1925 and Publ. Estac. Agron. Haina, Ser. B, Bot., 2: 12.
1926.
Holotype: Dominican Republic, Haina, on dry stems of Sida sp.
(Malvaceae), 28 Jun. 1925, R. Ciferri (MA 06453). Isotype: BPI
427458.
Lit.: Saccardo (1972: 1339).
Notes: Type material has been examined, but no Cladosporium
agreeing with the original description could be traced. The type
contains a mixture of several saprobic hyphomycetes.
solanicola Viégas, Bragantia 6: 368. 1946, as “solanicolum”.
Holotype: Brazil, Prov. St. Pauli, Campinas, Bosque de Jequìtibás,
on Solanum lycocarpum (Solanaceae), 27 Jun. 1913, A.P. Viégas
www.studiesinmycology.org
≡ Hormodendrum staurophorum W.B. Kendr., Canad. J. Bot. 39: 835.
1961.
≡ Devriesia staurophora (W.B. Kendr.) Seifert & N.L. Nickerson, Canad.
J. Bot. 82: 919. 2004.
stipae H.C. Greene, Trans. Wisconsin Acad. Sci. 41: 127.
1952. Fig. 392.
Lectotype (designated here): USA, Wisconsin, Dane Co.,
Madison, Univ. Wisconsin Arboretum, Oak opening, on living leaves
of Hesperostipa spartea (≡ Stipa spartea) (Poaceae), 6 Sep. 1951,
H.C. Greene (WIS). Isolectotype: BPI 427474.
≡ Stenella stipae (H.C. Greene) K. Schub. & U. Braun, Mycotaxon 92:
71. 2005.
≡ Zasmidium stipae (H.C. Greene) K. Schub. & U. Braun,
Schlechtendalia 20: 103. 2010.
Ill.: Schubert (2005a: 72, ig. 9).
“strictum Sacc.” - Gola (1930), Cladotrichum strictum Sacc.
was intended.
“stromatum Pers.” – listed by Saccardo & Berlese (1884:
100).
331
BenSch et al.
Lit.: Saccardo (1899: 1081), Ferraris (1912: 345), Schubert et al.
(2003: 99–100).
Notes: Syntype material is also deposited at NYS (USA, Kansas,
Rooks Co., Rockport, on living leaves of Populus monilifera, 17
Sep. 1894, E. Bartholomew, Kansas Fungi 1576).
supericiale Petch, Ann. Roy. Bot. Gard. (Peradeniya) 9: 327.
1925.
Holotype: Ceylon [Sri Lanka], Hakgala, on leaves of Cinnamomum
ovalifolium (Lauraceae), 27 Feb. 1922, No. 6570 (K 121571).
= Heteroconium solaninum (Sacc. & Syd.) M.B. Ellis, More Dematiaceous
Hyphomycetes: 65. 1976.
Lit.: Saccardo (1972: 1339).
Notes: In the type material, which has been examined, this fungus
is associated with a sooty mould. The conidiophores are solitary,
80–200 × 3–5 μm, pluriseptate, brown, with terminal conidiogenous
cells, often somewhat swollen, becoming intercalary by monopodial
proliferation, unilocal, conidia solitary or in short chains, ellipsoid,
subcylindrical, fusiform, 8–20 × 4–5 μm, mostly 3-septate.
symphoricarpi Dearn., in herb.
Fig. 392. Zasmidium stipae (WIS, lectotype of C. stipae). A. Conidia. B.
Conidiophores. Scale bar = 10 µm. K. Bensch del.
strumelloideum Milko & Dunaev, Novosti Sist. Nizsh. Rast.
23: 134. 1986.
Holotype: Russia, Yaroslavskaya Oblast, Rybinskoe, Sutka, on
leaves of Carex sp. (Cyperaceae) from stagnant water (BKMF2534). Ex-type culture: CBS 114484.
≡ Penidiella strumelloidea (Milko & Dunaev) Crous & U. Braun, Stud.
Mycol. 58: 23. 2007.
Ill.: Milko & Dunaev (1986: 135, ig. 1), Crous et al. (2007b: 23–24,
igs 14–15).
suaveolens (Lindner) Delitsch, Ergebnisse der theoretischen
und angewandten Mikrobiologie, Ed. Lembke, Bd. 1,
Systematik der Schimmelpilze: 135. 1943.
Type: In a distillery.
≡ Sachsia suaveolens Lindner, Mikroskopische Betriebskontrolle in den
Gährungsgewerben: 153. 1895.
≡ Oospora suaveolens (Lindner) Lindau, in Rabenhorst, Krypt.-Fl., ed.
2, 1(8): 35. 1907.
≡ Candida suaveolens (Lindner) Langeron & Guerra, ined. ?
≡ Geotrichum suaveolens (Lindner) Cif., Atti Ist. Bot. Lab. Crittog. Univ.
Pavia, Ser. 5, 19: 6. 1962. cited as “Cif., in Diddens & Lodder, 1942” ?.
≡ Moniliella suaveolens (Lindner) Arx, Antonie van Leeuwenhoek J.
Microbiol. Serol. 38(3): 294. 1972.
Notes: “The species does not belong in the genus Cladosporium”
(de Vries 1952: 97, with comments on type and other specimens).
subsessile Ellis & Barthol., Erythea 4: 83. 1896.
Lectotype (designated by Schubert et al. 2003): USA, Kansas, on
living leaves of Populus deltoides subsp. monilifera (≡ P. monilifera)
(Salicaceae), 18 Sep. 1894, Bartholomew (NY). Isolectotypes:
USA, Kansas, Rockport, on leaves of Populus deltoides subsp.
monilifera, Sep. 1894, E. Bartholomew, Ellis & Everhart, N. Amer.
Fungi 3288 (e.g., M, NY).
≡ Cladosporium brevipes Ellis & Barthol., Erythea 4: 27. 1896, nom. illeg.,
non C. brevipes Peck, 1887.
≡ Fusicladium subsessile (Ellis & Barthol.) K. Schub. & U. Braun, IMI
Descriptions of Fungi and Bacteria 152, No. 1519. 2002.
≡ Cladosporium maculicola Ellis & Barthol., in herb. (BPI 427256).
332
Specimen: Canada, British Columbia, Salmo, on Symphoricarpos acutus
(Caprifoliaceae), 11 Jul. 1935, G.G. Hedgcock (BPI 427503).
= Cercospora symphoricarpi Ellis & Everh., J. Mycol. 5: 70. 1889.
≡ Phaeoramularia symphoricarpi (Ellis & Everh.) Deighton, More
Dematiaceous Hyphomycetes: 317. 1976.
≡ Passalora symphoricarpi (Ellis & Everh.) U. Braun & Crous,
Mycosphaerella and its anamorphs. 1. Names published in Cercospora
and Passalora. CBS Biodiversity Ser. 1: 393. 2003.
Notes: As secondary invader Cladosporium herbarum s. lat. and
a cladosporioides-like Cladosporium species are occasionally
intermixed.
syphiliticum Hallier, Flora, Neu Reihe, 26(19): 294. 1868.
Type: Isol. from man associated with syphilis.
≡ Cladosporium coniothecii-syphilitici Hallier, Flora, Neue Reihe, 26(19):
294. 1868 (alternative name).
Lit.: Saccardo (1913a: 1371), Nannizzi (1934: 409).
Ill.: Hallier (1868a: tab. 3, ig. 13).
Notes: Introduced as state (morph) of Coniothecium syphiliticum
Hallier and Penicillium syphiliticum Hallier (p. 295). A doubtful, human
pathogenic fungus associated with syphilis, undoubtedly not belonging to
Cladosporium s. str. “Est species omnini obscura et vix Cladosporium.”
(Saccardo 1913a). “Species incertae” (Saccardo 1911: 282). Of E.
Hallier, “Herbarium and types: unknown” (Staleu & Cowan 1979).
tectonae Sawada, Rep. Gov. Res. Inst. Formosa 85: 92.
1943, nom. inval.
Syntypes: Taiwan, Taipei, on Tectona grandis (Lamiaceae), 6 May
1930, K. Sawada (BPI 427507, PPMH).
= Fusicladium tectonicola (Yong H. He & Z.Y. Zhang) U. Braun & K. Schub.
(see below).
≡ Cladosporium tectonicola Yong H. He & Z.Y. Zhang, Mycosystema
21(1): 21. 2002 and in Zhang et al., Flora Fungorum Sinicorum, Vol. 14:
164. 2003.
Notes: Description only in Japanese, not validly published.
tectonicola Y.H. He & Z.Y. Zhang, Mycosystema 21(1): 21.
2002 and in Zhang et al., Flora Fungorum Sinicorum, Vol.
14: 164. 2003.
Holotype: China, Guangdong, Ledong, on living leaves of Tectona
grandis (Lamiaceae), 30 Aug. 1978, D.R. Duan (HMAS 38603).
≡ Fusicladium tectonicola (Yong H. He & Z.Y. Zhang) U. Braun & K.
bensch, comb. nov. MycoBank MB800297.
the genuS Cladosporium
Basionym: Cladosporium tectonicola Yong H. He & Z.Y. Zhang,
Mycosystema 21(1): 21. 2002 and in Zhang et al., Flora Fungorum
Sinicorum, Vol. 14: 164. 2003.
= Cladosporium tectonae Sawada, Rep. Gov. Res. Inst. Formosa 85: 92.
1943, nom. inval.
Lit.: Schubert (2005a: 224).
Ill.: He & Zhang (2002: 21, ig. 1), Zhang et al. (2003: 165, ig. 114).
Notes: Zhang et al. (2003) cited Cladosporium tectonae as
synonym, which could be conirmed by a re-examination of
type material. This species is known from China (Gungdong
and Taiwan) on Tectona grandis. Furthermore, this species has
recently been found in Africa, also on T. grandis (Cameroon,
East Province, Dept. Lom et Djérem, between Gamboula and
Bazzama, ca. 23 km E of Bertoua, ca. 690 m alt., 4 Dec. 2007,
J. & M. Piątek, KRAM). This species is cladosporioid, but due to
unthickened, not darkened conidiogenous loci and conidial hila, it
is rather Pseudocladosporium-like. As a result of a phylogenetic
reassessment of venturiaceous anamorphs (Crous et al. 2007d),
Pseudocladosporium was reduced to synonym with Fusicladium.
Based on the examination of type material and the new collection
from Cameroon, F. tectonicola can be redescribed as follows:
On the lower leaf surface as dark olivaceous-brown to reddish
brown discolorations, effuse, at irst punctiform, later conluent,
often along leaf veins, on the upper side grey-brown. Colonies
hypophyllous, pale brown, villose, between leaf hairs. Mycelium
internal and external, supericial; hyphae branched, 1–5 μm wide,
septate, sometimes with swellings and slightly constricted at the
septa, very pale olivaceous, subhyaline, smooth, walls unthickened
or almost so. Stromata absent. Conidiophores solitary, arising from
hyphae, erect, straight, somewhat curved to lexuous, cylindricaloblong to iliform, unbranched, (20–)40–150(–300) × 3–6 μm,
pluriseptate, sometimes slightly constricted at the septa, pale to
medium dark brown, paler towards the apex, smooth, walls thin
to slightly thickened, ≤ 1 μm, often somewhat swollen at the base,
up to 10 μm wide. Conidiogenous cells integrated, terminal or
occasionally intercalary, 8–15 μm long, proliferation sympodial;
conidiogenous loci rather inconspicuous to subdenticulate, planate
to slightly convex, 0.5–1.5 μm wide, unthickened, not darkenedrefractive. Conidia catenate, in unbranched or branched chains,
straight, shape variable, limoniform, ellipsoid-ovoid, obovoid,
fusiform, subcylindrical, rarely subglobose, 5–15(–18) × 2–5 μm,
0(–1)-septate, subhyaline to very pale brown (occasionally with
a few ramoconidia distinguished from conidia and secondary
ramoconidia by being darker brown and have a broader truncate
base), smooth, thin-walled, apex rounded or with up to three apical
hila, base rounded to somewhat attenuated, hila truncate to slightly
convex, 0.5–1.5 μm wide, unthickened, not darkened, sometimes
slightly refractive.
Lit.: McGinnis & Borelli (1981), Ho et al. (1999: 146).
Notes: See C. bantianum.
trichoides C.W. Emmons var. chlamydosporum Kwon-Chung,
Mycologia 75(2): 320. 1983.
Type: USA, Maryland, from brain abscess in man.
= Cladophialophora bantiana (Sacc.) de Hoog, Kwon-Chung & McGinnis, J.
Med. Veterin. Mycol. 33: 343. 1995.
trichophilum H.C. Greene, Amer. Midl. Naturalist 48(3): 756.
1952, nom. illeg., non C. trichophilum Petr. & Cif., 1932. Fig.
393.
Lectotype (designated here): USA, Wisconsin, Rusk Co., Hawkins,
on living leaves of Lonicera hirsuta (Caprifoliaceae), 26 Aug. 1918,
J.J. Davis (WIS). Isolectotype: BPI 427512.
= Mycovellosiella nopomingensis B. Sutton, Mycol. Pap. 132: 77. 1973.
≡ Passalora nopomingensis (B. Sutton) U. Braun & Crous,
Mycosphaerella and its anamorphs: 1. Names published in Cercospora
and Passalora. CBS Biodiversity Ser. 1: 462. 2003.
Lit.: Shvartsman et al. (1975: 96), Schubert (2005a: 73–75).
Ill.: Schubert (2005a: 74, ig. 10).
trichophilum Petr. & Cif., Ann. Mycol. 30: 337. 1932.
Syntypes: Dominican Republic, Valle del Cibao, Prov. Santiago,
Las Lagunas, at Pozo Hediondo, on living leaves of Lantana
trifolia (Verbenaceae), 7 Dec. 1930, R. Ciferri & E.L. Ekman (BPI
427513A, 43696A, IMI 127138a, M-0057713, W).
≡ Mycovellosiella trichophila (Petr. & Cif.) Deighton, in herb. ?
= Cercospora lantanae Chupp, J. Dept. Agric. Porto Rico 15: 10. 1931. [Types:
CUP-PR 1200, IMI 132050].
≡ Mycovellosiella lantanae (Chupp) Deighton, Mycol. Pap. 137: 33. 1974.
≡ Passalora lantanae (Chupp) U. Braun & Crous, Mycosphaerella and
its anamorphs: 1. Names published in Cercospora and Passalora, CBS
Biodiversity Ser. 1: 242. 2003.
= Chaetotrichum lantanae Petr., Sydowia 5: 38. 1951, nom. nov., non
Chaetotrichum trichophilum (Stev.) Petr., 1951.
= Mycovellosiella lantanae var. verbenacearum K. Bhalla, S.K. Singh & A.K.
Srivast., Australas. Syst. Bot. 12: 369. 1999. [Type: IMI 373101].
tenerum (Link) E.W. Mason, in herb.?, Kirk et al. (n. d.) –
www.indexfungorum.org.
tenuis – Gola (1930: 21).
trichellum Sacc. – Gola (1930: 21).
trichoides C.W. Emmons, Amer. J. Clin. Pathol. 22: 541.
1952.
Type: USA, isol. from man.
= Cladophialophora bantiana (Sacc.) de Hoog, Kwon-Chung & McGinnis, J.
Med. Veterin. Mycol. 33: 343. 1995.
www.studiesinmycology.org
Fig. 393. Passalora nopomingensis (WIS, lectotype of C. trichophilum). Conidia and
conidiophores climbing leaf hairs. Scale bar = 10 µm. K. Bensch del.
333
BenSch et al.
Lit.: Saccardo (1895: 620), Lindau (1907: 828), Lind (1913: 524),
Oudemans (1923).
Notes: The fungus described by Hennings as C. uleanum is a
species of the genus Septoidium (hyphae broad, 5–10 μm wide,
medium brown, wall irregularly rugose; conidia formed by unilocal
conidiogenous cells, solitary, broadly ellipsoid-clavate, 50–80 ×
12–20 μm, 2-septate, wall pale, 1–2 μm wide, content golden-brown,
smooth, apex rounded, base subtruncate to convex, 7–10 μm wide,
cells often with distinct lumina, 5–15 μm diam.). This species agrees
well with Septoidium consimile, known from South American on
myrtaceous hosts, and is possibly an older name for this fungus.
unedonis Gonz. Frag., Mem. Real Acad. Ci. Barcelona, Ser.
3, 15(17): 459 (33). 1920.
Holotype: Spain, near Barcelona, Las Planas, on living leaves,
becoming dry, of Arbutus unedo (Ericaceae), 28 Mar. 1918, A.
Caballero (MA 06466).
Lit.: Gonzáles-Fragoso (1927: 204), Saccardo (1931: 790).
Notes: Excluded, taxonomic status unclear; Taeniolella/
Heteroconinium-like.
vagans (Pers.) Desm., Pl. Crypt. N. France, Ed. 1, Fasc. I,
No. 6. 1825, as “Pers.”.
Fig. 394. Fusicladium triostei (NYS 3219, holotype of C. triostei). Conidiophores and
conidia. Scale bar = 10 µm. K. Bensch del.
triostei Peck, Trans. Wisconsin Acad. Sci. 6: 119. 1885 and J.
Mycol. 1: 13. 1885. Fig. 394.
Holotype: USA, Wisconsin, La Crosse, on leaves of Triosteum
perfoliatum (Caprifoliaceae), L.H. Pammel (NYS 3219).
≡ Fusicladium triostei (Peck) K. Schub. & U. Braun, Mycol. Progr. 4(2):
102. 2005.
Lit.: Saccardo (1886: 359), de Vries (1952: 99).
Ill.: Schubert & Braun (2005a: 103, ig. 2).
tropicale Sartory, Bull. Acad. Roy. Méd. 113(24): 890. 1935,
as “tropicalis”, nom. inval.
Type: Central Africa, “Dermatomycosis tropicalis”, disease caused
in man, (location unknown).
Notes: Latin diagnosis lacking, description rudimentary. “Doubtful,
probably an Exophiala” (de Hoog et al. 2000: 1033) or an
Aureobasidium (Ciferri 1960: 501).
“typharum f. fuscum P. Karst.”, Hedwigia 35: 48, 1896.
Notes: Cited by Oudemans (1919); see C. typharum var. fuscum.
The actual reference in Hedwigia is to Brachysporium typharum
(Desm.) P. Karst. var. fuscum n. var.
“typharum var. fuscum P. Karst.”, Hedwigia 35: 48. 1896.
Lit.: Saccardo (1899: 1081).
Notes: See comment under Cladosporium typharum f. fuscum.
uleanum Henn., Hedwigia 34: 116. 1895.
≡ Septoidium uleanum (Henn.) U. Braun, comb. nov. MycoBank
MB800298.
Basionym: Cladosporium uleanum Henn., Hedwigia 34: 116. 1895.
= ? Septoidium consimile Arnaud, Ann. Épiphyt. 7: 62. 1921.
Lectotype (designated here): Brazil, Uberaba in Minas Gerais,
on living leaves of a Myrtaceae, Jun. 1892, E. Ule, no. 1927
(B 700006438). Isolectotype: HBG.
334
Type: On leaves of Acer, Tilia, etc.
≡ Fumago vagans Pers., Mycol. eur. 1: 9. 1822.
Lit.: Saccardo (1886: 370), Oudemans (1923), Friend (1965).
Notes: Fumago is a mixture of Aureobasidium pullulans, Cladosporium
spp. (mainly C. herbarum s. lat.) and sometimes additional saprobic
hyphomycetes, growing in honeydew secreted by aphids on the
upper leaf surface of trees (Friend 1965). See also C. fumago Link.
vangueriae (Thirum. & Mishra) Arx, Genera Fungi Sporul.
Pure Cult., Ed. 2: 222. 1974.
Types: India, Bihar, Darbhanga, on leaves of Meyna laxilora (≡
Vangueria spinosa) (Rubiaceae) (BPI 442756, IMI 51482).
Basionym: Biharia vangueriae Thirum. & Mishra, Sydowia 7(1–4):
79. 1963.
≡ Stenella vangueriae (Thirum. & Mishra) Deighton, Mycol. Pap. 144: 53.
1979.
≡ Zasmidium vangueriae (Thirum. & Mishra) Kamal, Cercosporioid
fungi of India: 252. 2010.
versicolor P.A. Dang., Botaniste 22: 455. 1931.
Type: France, in a glass of water containing a piece of potato
(location unknown).
Ill.: Dangeard (1931: 489, pl. 17; 491, pl. 18).
Notes: Dangeard (1931) described and illustrated this species as a
pycnidial fungus. Excluded.
versicolor T.E.T. Bond, Ceylon J. Sci., Sect. A, Bot. 12: 183.
1947, nom. illeg., non C. versicolor P.A. Dang., 1931.
Type: India, Ceylon [Sri Lanka], St. Coombs, on Ageratum
conyzoides (Asteraceae), Dec. 1943 (IMI 676).
= Cercospora perfoliati Ellis & Everh., J. Mycol. 5: 71, 1889, as “perfoliata”.
[Type: NY].
≡ Mycovellosiella perfoliati (Ellis & Everh.) Munt.-Cvetk., Lilloa 30: 201.
1960.
≡ Passalora perfoliati (Ellis & Everh.) U. Braun & Crous, Mycosphaerella
and its anamorphs: 1. Names published in Cercospora and Passalora,
CBS Biodiversity Ser. 1: 314. 2003.
= Cercospora agerati F. Stevens, Bernice P. Bishop Mus. Bull. 19: 154. 1925.
[Type: ILL 16297].
≡ Ragnhildiana agerati (F. Stevens) F. Stevens & Solheim, Mycologia 23:
402. 1931.
the genuS Cladosporium
= Ramularia agerati Sawada, Special Publ. Coll. Agric. Natl. Taiwan Univ. 8:
190. 1959, nom. inval. [Types: NTU-PPE, herb. Sawada, IMI 123997a (slide)].
= Cercosporella coorgica Muthappa, Mycopathol. Mycol. Appl. 34: 194. 1968.
[Type: IMI 937100].
Lit.: Deighton (1974: 69).
“virescens Pers.”, Mycol. eur. 1: 14. 1822.
Notes: Cladosporium virescens is cited in Lindau (1907: 206) as
synonym of Sporotrichum virescens (Pers.) Link (Bas.: Dematium
virescens Pers.) which seems to be an error. Persoon (1822) refers
to Dematium virescens; the name Cladosporium virescens Pers.
was never published.
virgultorum Schwein., Trans. Amer. Philos. Soc., N.S., 4(2):
277. 1832.
Syntypes: USA, Pennsylvania, Bethlehem, on branches, No. 2605
(PH 01020411, 01020412).
Lit.: Saccardo (1886: 356).
Notes: Excluded, not Cladosporium s. str., but identity not yet clear.
viticola Ces., Flora 37: 206. 1854 and in Klotzsch, Herb. Viv.
Mycol., Cent. XIX, No. 1877. 1854, as “viticolum”.
Syntypes: Italy, on Vitis sp. (Vitaceae), Klotzsch, Herb. Viv. Mycol.
1877 (e.g., BPI 797134, HAL).
≡ Cercospora viticola (Ces.) Sacc., Syll. fung. 4: 485. 1886.
= Pseudocercospora vitis (Lév.) Speg., Anales Mus. Nac. Buenos Aires 20:
438. 1910.
Lit.: Lindau (1910: 116, as “viticolum”), Chupp (1954: 605),
Sivanesan (1984: 210).
vitis (Lév.) Sacc., Mycoth. Ven., Cent. III, No. 284. 1875.
Type: Italy, Treviso, Selva, on leaves of Vitis vinifera (Vitaceae),
Sep. 1874, Saccardo, Mycoth. Ven. 284 (e.g., HAL).
Basionym: Septonema vitis Lév., Ann. Sci. Nat. Bot., Sér. 3, 9: 261.
1848.
≡ Cercospora vitis (Lév.) Sacc., Nuovo Giorn. Bot. Ital. 8: 188. 1876.
≡ Helminthosporium vitis (Lév.) Pirotta, Rev. Mycol. (Toulouse) 11: 185.
1889.
≡ Pseudocercospora vitis (Lév.) Speg., Anales Mus. Nac. Buenos Aires
20: 438. 1910.
≡ Phaeoisariopsis vitis (Lév.) Sawada, Rep. Dept. Agric. Gov. Res. Inst.
Formosa 2: 164. 1922.
≡ Cercosporiopsis vitis (Lév.) Miura, Flora of Manchuria and East
Mongolia, III. Cryptog. Fungi: 527. 1928.
= Cladosporium viticola Ces., Flora 38: 206. 1854 and in Klotzsch, Herb. Viv.
Mycol., Cent. XIX, No. 1877. 1854, as “viticolum”. [Type: e.g., HAL].
≡ Cercospora viticola (Ces.) Sacc., Syll. fung. 4: 485. 1886.
= Cladosporium ampelinum Pass., in Baglietto, Cesati & Notaris, Erb. Critt.
Ital., Ser. II, No. 595. 1872.
= Graphium clavisporium Berk. & Cooke, Grevillea 3(27): 100. 1874. [Type: K].
≡ Isariopsis clavispora (Berk. & Cooke) Sacc., Syll. fung. 4: 631. 1886.
= Cercospora vitis var. rupestris Cif., Ann. Mycol. 20: 45. 1922.
= ? Mycosphaerella personata B.B. Higgins, Amer. J. Bot. 16: 287. 1929.
= Cercospora vitis f. parthenocissi Docea, Lucr. Şti. Inst. Agron. ‘N. Bălescu’,
Ser. A., 11: 406. 1968.
Lit.: Lindau (1910: 116, as “vitis Sacc.”), Chupp (1954: 605),
Deighton (1976: 131), Sivanesan (1984: 210), Crous & Braun
(2003: 427).
werneckii Horta, Revista Med. Cirugía Brasil 29: 274. 1921,
as “Wernecki”.
Type: Brazil, on man. Ex-type culture: ATCC 36317.
≡ Dematium werneckii (Horta) C.W. Dodge, Med. Mycol.: 676. 1935.
≡ Pullularia werneckii (Horta) G.A. de Vries, Contr. Knowl. Genus
Cladosporium: 101. 1952.
≡ Exophiala werneckii (Horta) Arx, Genera Fungi Sporul. Pure Cult.: 180.
1970.
www.studiesinmycology.org
≡ Hortaea werneckii (Horta) Nishim. & Miyaji, Jap. J. Med. Mycol. 26(2):
145. 1984.
≡ Phaeoannellomyces werneckii (Horta) McGinnis & Schell, Sabouraudia
23(3): 184. 1985.
= Cryptococcus metaniger Castell., Archiv Dermatol. Syph. 16(4): 402. 1927.
≡ Cladosporium metaniger (Castell.) Ferraris, Atti Ist. Bot. “Giovanni
Briosi” 3: 183. 1932.
= Cladosporium rietmanni Sartory & Syd., Rev. Pat. Malad. Pays Chauds
15(1): 9–44. 1935.
Lit.: Nannizzi (1934: 408), de Vries (1952: 100), Cooke (1962: 34),
Kwon-Chung & Bennett (1992: 195), Schell (2003: 606).
wikstroemiae H. Zhang & Z.Y. Zhang, Proceedings of
Phytopathological Symposium Organized by Phytopathology
Laboratory of Yunnan Province 2: 306. 1998, nom. nov., as
“(Sawada) H. Zhang & Z.Y. Zhang comb. nov.”, nom. inval.
Lectotype: Taiwan, Taipei, on Wikstroemia indica (Thymelaeaceae),
19 Feb. 1913, K. Sawada (TNS F218930). Isolectotype: PPMH.
≡ Heterosporium wikstroemiae Sawada, Rep. Gov. Res. Inst. Formosa
87: 77. 1944, nom. inval. et illeg., non H. wikstroemiae Petch, 1922.
= Heterosporium wikstroemiae Petch, Ann. Roy. Bot. Gard. (Peradeniya) 7:
319. 1922. [Types: K, PAD].
≡ Stenella wikstroemiae (Petch) J. Walker, Mycol. Res. 95: 1010. 1991.
≡ Zasmidium wikstroemiae (Petch) U. Braun, comb. nov. MycoBank
MB800299.
Lit.: David (1997: 126).
Notes: Heterosporium wikstroemiae Sawada was published without
a Latin description.
zeae Peck, Rep. (Annual) New York State Mus. Nat. Hist. 46:
114. 1894.
Holotype: USA, Menands, on unripened grains of Zea mays
(Poaceae), Sep., C.H. Peck (NYS 3441).
Lit.: Saccardo (1895: 620).
Notes: Excluded, taxonomic status could not be clariied, only
sterile brown mycelium could be observed.
zizyphi P. Karst. & Roum., Rev. Mycol. (Toulouse) 12(46): 78.
1890, non Pseudocercospora zizyphi (Petch) Crous & Braun,
1996.
Syntypes: Vietnam, Tonkin, Hai Phong and “ad Sontag”, on faded
leaves of Zizyphus (Rhamnaceae), May 1888 and Dec. 1889 (B
700006769, PC and Roumeguère, Fungi Sel. Gall. Exs. 5500, e.g., B).
= Cercospora jujubae S. Chowdhury, Indian J. Agric. Sci. 16: 525. 1946. [Type:
IMI 113803].
≡ Pseudocercospora jujubae (S. Chowdhury) N. Khan & Shamsi,
Bangladesh J. Bot. 12: 117. 1983.
Lit.: Saccardo (1892: 604), Ferraris (1912: 342), Oudemans (1923),
Crous & Braun (2003: 233).
Unnamed Cladosporium states of named teleomorphs:
Cladosporium state of Apiosporina collinsii (Schwein.) Höhn.
≡ Fusicladium state of Apiosporina collinsii (Schwein.) Höhn.
Lit.: Sivanesan (1984: 598), Schubert (2001), Schubert et al. (2003:
105–106), Crous et al. (2007c: 205).
Notes: Using molecular sequence analyses, Crous et al. (2007c)
demonstrated that Apiosporina collinsii clusters in and belongs to
the Venturiaceae. They reduced Apiosporina to synonymy with
Venturia and its anamorph was referred to as Fusicladium. Zhang
et al. (2011) revealed Venturia to be polyphyletic, suggesting that
the anamorphs associated with Apiosporina are not members of
Venturia s. str.
335
BenSch et al.
Heterosporium
Lit.: Ellis (1976: 409), David (1997: 107).
Ill.: Ellis (1971: 410, ig. 318B).
The epithets are listed alphabetically. The generic name, i.e.,
Heterosporium, is dropped. Heterosporium was monographed by
David (1997), i.e., this list is mainly based on his examinations.
colocasiae Massee, J. Linn. Soc. London 24: 48. 1887.
adeniae Hansf., Proc. Linn. Soc. London 155: 46. 1943.
Lectotype (selected by David, 1997): Uganda, Kiagwe, on Adenia
cissampeloides (Passiloraceae), Jun. 1938, C.G. Hansford (IMI
10035). Isolectotype: K.
≡ Stenella adeniae (Hansf.) Deighton, Mycol. Pap. 144: 52. 1979.
≡ Zasmidium adeniae (Hansf.) U. Braun, comb. nov. MycoBank
MB800300.
Basionym: Heterosporium adeniae Hansf., Proc. Linn. Soc. London
155: 46. 1943.
= Cercospora adeniae J.M. Yen & Gilles, Bull. Soc. Mycol. France 90: 307.
1975.
Lit.: David (1997: 100).
albiziae (Petch) N. Naito, Mem. Coll. Agric. Kyoto Univ. 47:
51. 1940.
Holotype: Sri Lanka, Central Province, Perideniya, on Albizia
lebbek (Fabaceae), 21 Jun. 1908, Petch 2589 (K).
≡ Helminthosporium albiziae Petch, Ann. Roy. Bot. Gard. Perideniya 4(5):
51. 1940.
≡ Camptomeris albiziae (Petch) E.W. Mason, in Hansford, Proc. Linn.
Soc. London 155: 61. 1943.
Lit.: Ellis (1971: 286), David (1997: 100).
Ill.: Ellis (1971: 286, ig. 196).
allii var. bomareae Pat., Bull. Soc. Mycol. France 11: 233.
1895.
Holotype: Ecuador, Rio Machangara, on Bomarea sp.
(Alstroemeriaceae), 1892, G. de Lagerheim (F).
= Scolectotrichum alstroemeriae Allesch., Hedwigia 34: 116. 1895.
≡ Asperisporium alstroemeriae (Allesch.) Maubl., Lavoura 16: 211.
1913.
Lit.: David (1997: 100).
californicum Ellis & Everh., Fungi Columb., No. 1171. 1897,
nom. inval.
Syntypes: USA, California, on Eriodictyon californicum
(Boraginaceae), Ellis & Everhart, Fungi Columb. 1171 (e.g., K, NY).
= Heterosporium eucalypti var. maculicola (“maculicolum”) Ellis & Everh., N.
Amer. Fungi, Ser. II, No. 3491. 1896, nom. inval.
= Coniothecium eriodictyonis Dearn. & Barthol., in Dearness, Mycologia 21:
331. 1929.
≡ Trimmatostroma eriodictyonis (Dearn. & Barthol.) M.B. Ellis, More
Dematiaceous Hyphomycetes: 28. 1976.
Lit.: David (1997: 104–106).
Ill.: Ellis (1976: 29, ig. 13), David (1997: 105–106, igs 29 A–H, 30).
callospermum Speg., Anales Soc. Ci. Argent. 22: 213. 1886.
Holotype: Argentina, in ields near Santa Barbara and Villarica, on
spikes of Sporobolus sp. (Poaceae), Jan. 1882, C.L. Spegazzini
(LPS).
= Bipolaris ravenelii (M.A. Curtis) Shoemaker, Canad. J. Bot. 37: 884. 1959.
Lit.: David (1997: 107).
chloridis Speg., Revista Argent. Hist. Nat. 1(6): 430. 1891.
Holotype: Paraguay, in ields near Paraguarí, on Chloris sp.
(Poaceae), Feb. 1884, C.L. Spegazzini 4311 (location unknown,
not at LPS).
≡ Acroconidiellina chloridis (Speg.) M.B. Ellis, Mycol. Pap. 125: 24.
1971.
336
Holotype: Jamaica, Portland Parish, near Priestman’s River, on
Colocasia esculenta (Araceae), without any date and collector
(location unknown).
Lit.: David (1997: 107).
Ill.: Massee (1887: pl. 1, ig. 2).
Notes: Excluded, no Cladosporium, but status unclear due to
depauperate type material. According to David (1997), this fungus
could either be Trichocladium asperum Harz or Johnstonia
colocasiae M.B. Ellis. A mixture of these two species should also
be taken into consideration.
dalmaticum Jaap, Ann. Mycol. 14: 43. 1916.
Holotype: Montenegro, Herceg Novi (Castelnuovo), Zelenika, on
rotting stems of Phytolacca americana (Phytolaccaceae), 30 Apr.
1914, O. Jaap 307 (HBG).
Lit.: David (1997: 108).
Notes: Status unclear.
dianellae Sawada, Rep. Gov. Res. Inst. Formosa 87: 76.
1944, nom. inval.
Holotype: Taiwan, Taipei, on Dianella ensifolia (Xanthorrhoeaceae),
19 Nov. 1925, K. Sawada (NTU-PPE).
= Cercospora dianellae Sawada & Katsuki, Special Publ. Coll. Agric. Natl.
Taiwan Univ. 8: 216. 1959.
≡ Stenella dianellae (Sawada & Katsuki) Goh & W.H. Hsieh, Trans.
Mycol. Soc. Republ. China 2: 137. 1987.
≡ Zasmidium dianellae (Sawada & Katsuki) U. Braun, comb. nov.
MycoBank MB800301.
Basionym: Cercospora dianellae Sawada & Katsuki, Special Publ.
Coll. Agric. Natl. Taiwan Univ. 8: 216. 1959.
Lit.: David (1997: 108–110), Goh & Hsieh (1990: 209).
Ill.: David (1997: 110, fig. 31), Goh & Hsieh (1990: 210, fig.
162).
eschscholtziae Harkn., Bull. Calif. Acad. Sci. 1: 38. 1884.
Holotype: USA, California, San Francisco, on Eschscholtzia
californica (Papaveraceae), Jan. 1884, Harkness 3116 (not
preserved).
≡ Acroconidiella eschscholtziae (Harkn.) M.B. Ellis,
Dematiaceous Hyphomycetes: 407. 1976.
≡ Cladosporium eschscholtziae (Harkn.) Dingley, nom. ined.
More
Lit.: David (1997: 111).
Ill.: Ellis (1976: 408, ig. 317).
eucalypti var. maculicola (“maculicolum”) Ellis & Everh., N.
Amer. Fungi, Ser. II, No. 3491. 1896, nom. inval.
Syntype: USA, California, San Gabriel, on Eriodictyon californicum
(Boraginaceae), Jan. 1896, A.J. McClatchie, Ellis & Everhart, N.
Amer. Fungi 3491 (e.g., NY).
= Heterosporium californicum Ellis & Everh., Fungi Columb., No. 1171. 1897,
nom. inval.
= Coniothecium eriodictyonis Dearn. & Barthol., in Dearness, Mycologia 21:
331. 1929.
≡ Trimmatostroma eriodictyonis (Dearn. & Barthol.) M.B. Ellis, More
Dematiaceous Hyphomycetes: 28. 1976.
Lit.: David (1997: 104–106).
Ill.: Ellis (1976: 29, ig. 13), David (1997: 105–106, igs 29 A–H,
30).
the genuS Cladosporium
gramineum (Rabenh. ex Schltdl.) J. Schröt., in Cohn, Kryptl.
Schlesien 3(2): 499. 1897.
≡ Helminthosporium gramineum Rabenh. ex Schltdl., Bot. Zeitung
(Berlin) 15: 94. 1857.
≡ Drechslera graminea (Rabenh. ex Schltdl.) Shoemaker, Canad. J.
Bot. 37: 881. 1959.
Lit.: Ellis (1971: 428), David (1997: 112).
Ill.: Ellis (1971: 428, ig. 298).
granulatum (Berk. & M.A. Curtis) Cooke, Grevillea 5: 123.
1877.
Holotype: Cuba, on dead herbaceous stems, Wright 795, ex herb.
Berkeley, Cuban Fungi 631 (K).
≡ Helminthosporium granulatum Berk. & M.A. Curtis, in Berkeley, J. Linn.
Soc. London 10: 361. 1868.
= Dendryphiella vinosa (Berk. & M.A. Curtis) Reisinger, Bull. Trimestriel Soc.
Mycol. France 84: 27. 1968.
Lit.: Ellis (1971: 500), David (1997: 112).
Ill.: Ellis (1971: 499, ig. 358).
interseminatum (Berk. & Ravenel) G.F. Atk., Cornell Univ.
Sci. Bull. 3: 48. 1897.
≡ Helminthosporium interseminatum Berk. & Ravenel, Grevillea 3: 103.
1875.
≡ Dendryphiella interseminata (Berk. & Ravenel) Bubák & Ranoj., Ann.
Mycol. 12: 417. 1914.
≡ Dendryphion interseminatum (Berk. & Ravenel) S. Hughes, Canad. J.
Bot. 31: 638. 1953.
= Dendryphiella vinosa (Berk. & M.A. Curtis) Reisinger, Bull. Trimestriel Soc.
Mycol. France 84: 27. 1968.
Lit.: Cooke (1950), Ellis (1971: 500), David (1997).
Ill.: Ellis (1971: 499, ig. 358).
lagunense Syd. & P. Syd., Ann. Mycol. 18: 104. 1920.
Type: Philippines, Los Baños, on Cajanus cajan (Fabaceae), 3
Nov. 1919, F.B. Serrano 6278 (location unknown, neither S nor
B).
= ? Dendryphiella vinosa (Berk. & M.A. Curtis) Reisinger, Bull. Trimestriel
Soc. Mycol. France 84: 27. 1968.
Lit.: David (1997).
lilacis (Desm.) Puttemans, Notes Phytopathol. Mycol.
Bruxelles: 2. 1918.
Syntypes: France, Pas-de-Calais, Arras, on Syringa vulgaris
(Oleaceae), Desmazières, Pl. Crypt. N. France 1850 (e.g., K, PC).
≡ Exosporium lilacis Desm., Ann. Sci. Nat. Bot., Sér. 3, 11: 364. 1849.
≡ Cercospora lilacis (Desm.) Sacc., Michelia 2: 128. 1880.
≡ Pseudocercospora lilacis (Desm.) Deighton, Trans. Brit. Mycol. Soc.
88: 389. 1987.
Lit.: David (1997: 113), Crous & Braun (2003: 251).
magnoliae Weedon, Mycologia 18: 222. 1926.
Holotype: USA, Florida, St. Petersburg, on Magnolia grandilora
(Magnoliaceae), 15 Feb. 1923, A.G. Weedon, Fungi of Florida 1
(ILL 16167).
≡ Stenellopsis magnoliae (Weedon) Morgan-Jones, Mycotaxon 10: 406.
1980.
≡ Parastenella magnoliae (Weedon) J.C. David, Mycol. Res. 95: 124.
1991.
Lit.: David (1997: 113).
Ill.: David (1997: 114, ig. 32).
munduleae Syd. & P. Syd., Ann. Mycol. 10: 45. 1912.
Holotype: South Africa, Transvaal, Koedoesriver, Zoutpansberg,
on Mundulea sericea (Fabaceae), 10 Aug. 1911, E. M. Doidge (S).
Isotype: IMI 38033.
www.studiesinmycology.org
≡ Sirosporium munduleae (Syd. & P. Syd.) M.B. Ellis, More
Dematiaceous Hyphomycetes: 302. 1976.
Lit.: David (1997: 115).
Ill.: Ellis (1976: 302, ig. 227).
paradoxum Syd. & P. Syd., in Fuhrmann & Mayor, Mém. Soc.
Neuchâtel. Sci. Nat. 5: 441. 1914.
Holotype: Colombia, Antioquia Dép., Guaca, on Calea sessililora
(= C. glomerata) (Asteraceae), 12 Sep. 1910, E. Mayor (S).
≡ Laocoön paradoxus (Syd. & P. Syd.) J.C. David, Mycol. Pap. 172:
116. 1997.
Ill.: David (1997: 117–119, igs 33–35).
paulsenii Rostr., Bot. Tidskr. 28: 218. 1907.
Holotype: Tadzhikistan, Pamir Mts., on Arnebia euchroma (≡
Macrotomia euchromon) (Boraginaceae), 22 Jul. 1898, O. Paulsen
864 (C).
Lit.: David (1997: 120).
Notes: Not Cladosporium, but status unclear, probably a
Scolecobasidium.
phragmitis var. ammophilae Grove, in herb.
Material examined by David (1997): UK, Cheshire, Leasowe, on
old leaves of Ammophila arenaria (Poaceae), 15 Oct. 1921, V.S.J.
(K).
= Scolecobasidium arenarium (Nicot) M.B. Ellis, More Dematiaceous
Hyphomycetes: 194. 1976.
Lit.: David (1997: 120).
repandum Ferd. & Winge, Bot. Tidskr. 29: 23. 1908.
Holotype: West Indies, St. Thomas, Lovenlund, on dry branches,
16 Dec. 1905, C. Raunkiær (C).
= Dendryphiella vinosa (Berk. & M.A. Curtis) Reisinger, Bull. Trimestriel Soc.
Mycol. France 84: 27. 1968.
Lit.: Ellis (1971: 500), David (1997: 120).
Ill.: Ellis (1971: 499, ig. 358).
sambuci Earle, Bull. Torrey Bot. Club 20: 30. 1897.
Holotype: USA, Alabama, Lee Co., Auburn, on dead stems of
Sambucus sp. (Adoxaceae), 13 Mar. 1896, F.S. Earle & J.M.
Underwood (NY).
= Dendryphiella vinosa (Berk. & M.A. Curtis) Reisinger, Bull. Trimestriel Soc.
Mycol. France 84: 27. 1968.
Lit.: Ellis (1971: 500), David (1997: 120).
Ill.: Ellis (1971: 499, ig. 358).
secalis Dippen., S. African J. Sci. 28: 286. 1931.
Holotype: South Africa, Cape, Stellenbosch, on Secale cereale
(Poaceae), 25 Sep. 1929, BJ Dippenaar (PREM 46907).
= Passalora graminis (Fuckel) Höhn., Zentralbl. Bakteriol. Parasitenk., Abt.
2, 60: 6. 1923.
Lit.: David (1997: 121), Crous & Braun (2003: 203).
selaginellarum M.L. Farr, in Farr & Horner, Nova Hedwigia
15: 266. 1968.
Holotype: USA, Florida, Highlands Co., Archibold Biological
Station, 10 miles S of lake Placid, on Selaginella arenicola
(Selaginellaceae), 22 Jan. 1945, Morton (BPI 802195). Isotype: F.
Lit.: David (1997: 121).
Ill.: David (1997: 122, ig. 36).
Notes: Generic afinity unclear.
337
BenSch et al.
terrestre (E.V. Abbott) R.G. Atk., Mycologia 44: 813. 1952.
Holotype: Canada, Ontario, Ancaster, isol. from soil, 31 Oct. 1947,
RG Atkinson (DAOM 28282).
Holotype: USA, California, Anderson, Coleman Fisheries
Station, isol. ex mycoses of the kidney of the ish Onchorhyncus
tschawytscha, without date and locality (BPI).
Lit.: David (1997: 123).
Notes: All names described in the genus Ochroconis will be revised
in a paper by de Hoog and co-workers which will be published in
Persoonia.
Lit.: David (1997: 125).
Notes: See comments on the genus Ochroconis above under H.
terrestre.
= Scolecobasidium constrictum E.V. Abbott, Mycologia 19: 30. 1927.
≡ Ochroconis constricta (E.V. Abbott) de Hoog & Arx, Kavaka 1: 57.
1973.
tropaeoli T.E.T. Bond, Ceylon J. Sci., Sect. A, Bot. 12: 185.
1947.
Holotype: Sri Lanka, St. Coombs, Talawakelle, on Tropaeolum
majus (Tropaeolaceae), 9 Mar. 1944, T.E.T. Bond (TRI 359).
Isotype: IMI 677.
≡ Scolecobasidium tschawytschae (Doty & D.W. Slater) McGinnis &
Ajello, Trans. Brit. Mycol. Soc. 63: 202. 1974.
≡ Ochroconis tschawytschae (Doty & D.W. Slater) Kiril. & Al-Achmed,
Mikrobiol. Zhurn. 39: 305. 1977.
wikstroemiae Petch, Ann. Roy. Bot. Gard. Perideniya 7: 319.
1922.
Holotype: Sri Lanka, Perideniya, on Wikstroemia indica (= W.
viridilora) (Thymelaeaceae), 2 Feb. 1919, T. Petch 5878 (PDA).
Isotype: K.
≡ Acroconidiella tropaeoli (T.E.T. Bond) J.C. Lindq. & Alippi, Darwiniana
13: 613. 1964.
≡ Stenella wikstroemiae (Petch) J. Walker, Mycol. Res. 95: 1010. 1991.
≡ Zasmidium wikstroemiae (Petch) U. Braun, comb. nov. MycoBank
MB800299.
tschawytschae Doty & D.W. Slater, Amer. Midl. Naturalist 36:
663. 1946.
Basionym: Heterosporium wikstroemiae Petch, Ann. Roy. Bot.
Gard. Perideniya 7: 319. 1922.
Lit.: David (1997: 126).
Ill.: David (1997: 127, ig. 38).
Notes: See list of excluded Cladosporium species.
Lit.: Ellis (1971: 462), David (1997: 125).
Ill.: Ellis (1971: 461, ig. 328).
338
the genuS Cladosporium
UNCERTAIN AND DOUBTFUL
CLADOSPORIUM S. LAT.
SPECIES
OF
Type material of the following taxa could not be traced, was not
available or too sparse for a inal conclusion, so that the generic
afinity and taxonomic status of these taxa could not be proven and
remain unclear. Original descriptions and illustrations, if present and
available, are reproduced. The particular epithets are alphabetically
listed, but the generic name Cladosporium is not applied. Numerous
new species have recently been described from China by Z.Y. Zhang
and co-authors (Zhang et al. 2003), using a rather broad, traditional
concept of Cladosporium s. lat. Type collections are deposited at
MHYAU, but all attempts to re-examine these collections, including a
visit of the herbarium concerned, failed, i.e. the access to the material
was refused. Hence, the species described by Z.Y. Zhang and coauthors have to be included in the list of uncertain and doubtful
species. For illustrations of published species consult MycoBank.
Cladosporium
acerinum Noelli, Nuovo Giorn. Bot. Ital., N.S. 24(3): 195.
1917.
Type: Italy, Piemont, Bruere (Rivoli), on branches of Acer negundo
(Aceraceae), 29 Mar. 1915.
Lit.: Saccardo (1931: 787).
Original diagnosis (Noelli 1917): Caespitulis effuses, minutis,
aterrimis, inaequalibus; conidiophoris ramosis, dilute olivaceis,
aggregatis, septatis, lexuosis; conidiis copiosis, initio oblongis et
simplicibus, olivaceis, deinde ovatis, 1-septatis, constrictis, obtusis
vel acutis, 20–27 × 10–12 μm, fuscis.
Notes: Noelli (1917) compared his newly introduced species with
C. epiphyllum, a synonym of C. herbarum, which differs however
in having narrower conidia. Cladosporium acerinum could rather
belong to C. macrocarpum, if it is a member of the C. herbarum
complex at all. Cladosporium epiphyllum var. acerinum collected
on leaves of Acer platanoides in the USA proved to be synonymous
with C. macrocarpum (Schubert 2005b).
aeruginosum F. Patt., Bull. Torrey Bot. Club 27: 284. 1900.
Type: USA, Department of Agriculture, greenhouse, on living
leaves of Osmanthus fragrans (Olea fragrans) (Oleaceae) affected
by Gloeosporium oleae, Jan. 1900, F.W. Patterson (type in herb. of
“Division of Vegetable Physiology and Pathology, U.S. Department
of Agriculture”). Topotype: 6 Feb. 1900 (BPI 426094).
Lit.: Saccardo (1902: 1058).
Original diagnosis (Patterson 1900): Tufts epiphyllous, verdigris
green, densely fasciculate, distinct, upon light-coloured arid spots;
hyphae simple, septate, nodulose, very light green, 45–90 × 3–3.5
μm; conidia lateral and terminal, in chains of 5 or more, almost
hyaline, generally oblong and continuous, sometimes oblongelliptical and once or twice septate, 5–12 × 2–2.5 μm.
Notes: Type material of C. aeruginosum could not be located.
The collection from BPI can be considered as topotype material,
but could not be examined during the course of the recent
morphotaxonomic studies.
albiziae S.N. Khan & B.M. Misra, Indian Forester 125(7): 746.
1999.
Holotype: India, Uttar Pradesh, Kalsi (Dehra Dun), on leaves of
Albizia lebbek (Fabaceae) (IMI 282484).
Ill.: Khan & Misra (1999: 745, ig. 2).
www.studiesinmycology.org
Original diagnosis (Khan & Misra 1999): Conidiophora simplica,
raro ramosa, brunnea ad atro-brunnea ad cinereo-brunneae,
septata, parietibus parum crassibus, levi, 60–180 × 5–7 μm.
Ramoconidia pallida brunnea, 0–3 septata, ovalia vel cylindrica,
16–22 × 4–5 μm. Conidia pallida-brunnea, cylindrica, levi, 8–12
× 5–6 μm.
Notes: Khan & Misra (1999) reported this species as being allied
to C. psoraleae but different in its virulence, lesion formation and
conidial characters. However, type material, cited to be deposited
at IMI, could not be traced and is probably not preserved.
alliorum Hanzawa, Mycol. Centralbl. 5: 11. 1914.
Type: Japan, Sapporo, on leaves of Allium cepa (Amaryllidaceae).
Lit.: Saccardo (1931: 793).
Ill.: Hanzawa (1914: 6, ig. 2).
Original diagnosis (Hanzawa 1914): Der Pilzrasen besteht aus
vielen Conidienträgern, er entspringt aus den Spaltöffnungen der
Blätter. Die Conidienträger sind unverzweigt, schwach gebogen,
stellenweise etwas angeschwollen, bräunlich gefärbt, vacuoliert.
Ihre Länge ist verschieden, bis 135 μm lang, 4–6 μm (auch 10
μm breit), angeschwollene 8 μm breit. Conidien oval, elliptisch,
besitzen kleine Auswüchse an der Spitze, Oberläche feinpunctiert,
gelb-bräunlich, ohne oder mit 1–2 Septen. Größe verschieden,
meistens 14–25 μm lang, 12–17 μm breit.
Notes: Type material of this species could not be traced. Hanzawa
(1914) described this species as closely related to Cladosporium
herbarum, but distinct by having somewhat swollen conidiophores
and thick conidia. Based on the description and igure given
above, C. alliorum is possibly identical with C. macrocarpum, but
the conidia of C. alliorum are somewhat wider, well agreeing with
the conidial width in C. allii, which has, however, much longer
conidia. Cladosporium allii-cepae, occurring on Allium cepa,
possesses wider conidiophores and usually solitary, much longer
conidia.
alpiniae T. Zhang & Z.Y. Zhang, Plant Diseases and Their
Control: 108. 1998.
Holotype: China, Hubei, Wuchang, on living leaves of Alpinia
galanga (Zingiberaceae), 22 Sep. 1980, J.Y. Li & T.Y. Zhang, No.
4074 (MHYAU 03947).
Ill.: Zhang & Zhang (1998b: 109, ig. 1), Zhang et al. (2003: 38, ig.
12; pl. 10, ig. 2).
Original diagnosis (Zhang & Zhang 1998b): Maculis amphigenis,
apicis vel marginis, atro-brunneis, longi-striatis, marginalibus
pallide brunneis longi-undulatis ornatis, spot blight usque ad 18 ×
1.2 cm vel 3–5 × 1.2 cm, olivaceis mucoris amphigenis, inusitatis.
Conidiphoris solitariis vel rarius ramosis, erectis, septatis,
sympoditer proliferis denticulatis vel cicatrisosis, brunneis, prope
apicem pallescentis, 11.5–219.0 × 2.6–4.6 μm. Ramoconidiis
0–1-septatis, rarius 2–3-septatis, cicatricibus protrudentibus,
praeditis, pallide brunneis, 5.0–18.0 × 3.3–4.6 μm. Conidiis
catenatis, fusiformis, subsphaericis, continuis, laevis, pallide
brunneis, 2.6–7.2 × 2.6–5.1 μm.
Notes: Zhang & Zhang (1998b) cited Alpinia zerumbet as
an additional host species and compared C. alpiniae with C.
cladosporioides, the only other species occurring on a host
belonging to the Zingiberaceae (on Zingiber oficinale), stating
that the latter species possesses longer, limoniform or ellipsoid
conidia. Since type material was not available the status of this
taxon remains unclear.
339
BenSch et al.
ambrosiae House, in herb.
Specimen: USA, New York, Albany Co. Selkirk, on dead stems of Ambrosia triida
(Asteraceae), 10 Apr. 1925, H.D. House (NYS).
amphitrichum Sacc., Syll. fung. 4: 354. 1886.
Type: Czech Republic, near Reichenberg, on rotten wood of Pinus
sp. (Pinaceae).
≡ Amphitrichum olivaceum Corda, Icon. fung. 1: 16. 1837. non C.
olivaceum (Corda) Bonord., 1851.
Lit.: Lindau (1907: 811).
Ill.: Corda (1837: tab. 4, ig. 221).
Original diagnosis (Saccardo 1886): Caespitulis effusis, oblongis
olivaceis; stromatibus aggregatis, convexis, olivaceis opacis,
mycelio spurio suffultis; hyphis simplicibus, gracilibus, lexilibus,
pauciseptatis, olivaceis, tectis; conidiis oblongis, simplicibus vel
didymis, obtusis, pellucidis, 9.5 μm crass.
Notes: Type material could not be traced at PRM. A collection from
Austria, deposited as C. amphitrichum, proved to be C. herbarum
s. lat. (on dead wood of Picea abies, Austria, Tirol, near Lienz, Jun.
1922, K. Keissler, BPI 426111). However, in the brief description
of this species the conidial wides was given to be 9.5 μm. Hence,
C. amphitrichum could rather belong to C. macrocarpum, if it is a
member of the C. herbarum complex at all.
aphidis var. muscae Briard & Har., Rev. Mycol. (Toulouse)
12: 132. 1890.
Type: France, Méry-sur-Seine, on a dead carcass of a ly (Musca),
P. Hariot.
Lit.: Saccardo (1892: 605).
Original diagnosis (Briard & Hariot l.c.): Hyphes droits, rameux,
fascicules, les uns continus et les autres septés légèrement
fuligineux, diaphanes, 5–7 micro. diam; Conidies nombreuses,
ovales ou ovales-oblongues, apiculées au point d’attache et
souvent aux deux extrémités, simples ou 1–2 septées, de meme
couleur que les hyphes ou un peu plus pales, mesurant: les simples
6–8 × 4–5, les didymes 20–22 × 7–8.
Notes: Type material could not be traced. Status remains unclear.
A single collection from Hungary on dead carcasses of Musca sp.
proved to be phylogenetically conspeciic with C. fusiforme.
apiculatum Berk., in herb.
Specimen: USA, Carolina, on Helianthus sp. (Asteraceae) (K).
Lit.: Saccardo (1895: 621).
aquilinum, in herb.
Specimen: On Pteridium sp. (Dennstaedtiaceae); on Pteridium, Northampton
(PH).
arcticum Berl. & Voglino, Syll. fung. 4a: 170. 1886.
Type: Denmark, Greenland, Kaiser-Franz-Joseph-Fjord, on
Epilobium latifolium (Onagraceae).
Lit.: Saccardo (1891: 882).
Original diagnosis (Berlese & Voglino 1886): Caespitulis minutis,
maculaeformibus, olivaceis, velutinis; hyphis subsimplicibus,
cylindraceis, septatis, sub lente fuscis, apice conidiophores;
conidiis oblongo-ovatis vel subclavatis, 1–2-septatis, quandoque
oblique pedicellatis, lavo-fuscis, 24 × 8.
Notes: The species was described by Berlese & Voglino (1886) as
anamorphic state of Pleospora arctica Fuckel. In a collection from B
(Greenland, Umanakfjord, on dead leaves of Arabis hookeri, 14 Jul.
1892, E. Vanhöffen, B 700006170), referred to as C. arcticum, only
C. herbarum and other saprobic hyphomycetes have been found.
340
aristolochiae H. Zhang & Z.Y. Zhang, Mycosystema 17(4):
304. 1998.
Holotype: China, Hubei, Wuchang, on living leaves of Aristolochia
kwangsiensis (Aristolochiaceae), 22 Sep. 1980, J.Y. Li & T.Y. Zhang
(MHYAU 03956).
Ill.: Zhang & Zhang (1998a: 304, ig. 1), Zhang et al. (2003: 46, ig.
19).
Original diagnosis (Zhang & Zhang 1998a): Maculae amphigenae,
lavo-brunneae vel griseae, longe undulatae, atro-brunneae,
4.5 × 8.0 cm. Conidiophora solitaria, erecta, simplicia, septata,
sympodialia cicatricosa brunnea, sursum pallide brunnea, 39–90 ×
3.7–3.8 μm. Ramoconidia 0–1 interdum 3-septata, ellipsoidea vel
ellipsoideo-cylindrica, pallide brunnea, cicatricibus conspicuis, 3.9–
22 × 3.9–9.1 μm. Conidia catenata, continua vel raro 1-septata,
subsphaerica vel ellipsoidea, laevia, pallide brunnea, 2.8–11.1 ×
2.8–5.1 μm.
arundinaceum Mont., Ann. Sci. Nat. Bot., Sér. 3, 12: 299.
1849.
Type: France, Marseille, on sheaths and leaves of Arundo
mediterranea (= A. mauritanica, Ampelodesmos mauritanicus)
(Poaceae), Castagne.
Lit.: Saccardo (1886: 364).
Original diagnosis (Montagne 1849): Fibris erumpentibus lineatim
seriatis atris apice fasciculato-ramosis ramisque strictis inaequaliter
septatis, sporis simplicibus et rotundo ovatis tandem fuscis.
Notes: Type material could not be traced, but all collections referred
to as “C. arundinaceum” (e.g., B 700006173–700006175) proved
to be C. herbarum.
arundinicola Berl., Riv. Patol. Veg. 4: 19. 1895, as
“arundinicolum”.
Type: Italy, Avellino, on rotting culms of Arundo donax (Poaceae).
Lit.: Saccardo (1913a: 1371), Ferraris (1914: 883), GonzálesFragoso (1927: 210).
Ill.: Berlese (1895: tab. 3, ig. 17).
Original diagnosis (Berlese 1895): Esso é del tutto supericiale,
di colore tra il fulvo e l’olivaceo, e costituisce uno spesso ifasma
crostaceo alla supericie del substrato. I conidiofori sono quá e cola
raccolti in cespuglietti piuttosto compatti, hanno pure un maggiore
o minore numero di nodi. I conidi, che abbondantissimi compaiono
sotto al microscopio, sono in generale piuttosto allungati, muriculati,
continui o divisi da uno, due e perino cinque setti trasversali. Ciò
si osserva specialmente nei ramo-conidi direttamente inseriti
sul conidiofora, i quail hanno forma decisamente cilindrica od
alquanto fusoidea e rispondono alle dimensioni di 20–26 × 6–8,
non mancano conidi quasi rotondi (gli apicali) per cui l’apparato
conidiale va compreso fra le seguenti dimensioni 6–26 × 4–8.
Notes: Type material could not be traced, status remains unclear.
asteromatoides Sacc., Atti Reale Ist. Veneto Sci. Lett. Arti,
Ser. 6, 3(4): 722. 1885. Fig. 395.
Type: Tahiti, on legumes of Erythrina sp. (?) (Fabaceae), May
1884, G. Brunaud, Roumeguère, Fungi Sel. Gall. Exs. 3292 (e.g.,
B, FH, PAD).
≡ Cladosporium asteromatoides Sacc. & Roum., in Roumeguère, Fungi
Sel. Gall. Exs., Cent. 33, No. 3292. 1885, nom. illeg., homonym [host
given as “Cassia sp.” and authors as “Sacc. & Roum.”].
Lit.: Saccardo (1886: 353).
Original diagnosis (Saccardo & Berlese 1885): Caespitulis
adpressis, subradiantibus, asteromatoideis, nigris minutis; hyphis
the genuS Cladosporium
Notes: Status unclear. No material traced at Kew (K).
atroseptum Pidopl. & Deniak, in Pidoplichko, Gribnaya Flora
Grubykh Kormov: 268. 1953, nom. inval.
Type: Ukraine, isolated from damp straw.
Ill.: Pidoplichko (1953: 268, ig. 69).
Notes: Type material could not be traced, possibly not maintained.
The diagnosis is given in Russian.
autumnale Kübler, Arch. Sci. Phys. Nat., Sér. 3, 2: 699. 1879.
Type: Switzerland, on leaves of Vitis sp. (Vitaceae), 1876, M.
Kübler.
Notes: Type material could not be traced.
Fig. 395. Cladosporium asteromatoides (PAD). Conidiophores and conidia in vivo.
Scale bar = 10 µm. K. Bensch del.
varie ramosis, ad basim contextis, septulatis, olivaceo-fuscis;
conidiis ellipsoideis, minutis, 5.7 × 2.5 continuis v. 1-septatis.
Description based on the holotype (PAD): On pods which
appear to be deformed, spots numerous, scattered to subeffuse,
punctiform or as small patches, often growing dendritic in the
peripherie, sometimes conluent, up to 3 mm diam, mostly smaller,
sometimes slightly raised in the centre, brownish to somewhat
blackish, often somewhat darker in the centre. Colonies scattered
to subeffuse, shortly caespitose, often not very conspicuous,
brownish, somewhat velvety. Mycelium internal, subcuticular to
intraepidermal; hyphae branched, 2–10 μm wide, pluriseptate,
often with swellings and constrictions, pale olivaceous brown,
darker when aggregated, medium brown, smooth, walls somewhat
thickened, forming extended often radiating hyphal plates,
loose to dense, appearing pseudoparenchymatous, cells often
somewhat irregular, sometimes more or less lobed. Stromata welldeveloped, extended, dense, up to 100 μm diam, cells subglobose
to somewhat angular-polygonal, 4–10 μm wide, medium brown,
smooth, thick-walled. Conidiophores solitary or in loose groups,
arising from stromata, erumpent, erect, straight to more or
less lexuous, somewhat geniculate-sinuous, non-nodulose,
unbranched, occasionally branched, 14–50 × 3–4(–5) μm, septate,
not constricted, pale brown, smooth to somewhat rough-walled,
walls unthickened or almost so. Conidiogenous cells integrated,
terminal and intercalary, cylindrical, slightly geniculate, 8–20 μm
long, wih a single or few conidiogenous loci, protuberant, 0.5–1.5(–
2) μm diam, thickened, somewhat darkened-refractive. Conidia
catenate, in unbranched or branched chains, straight, subglobose,
ovoid, obovoid, ellipsoid to subcylindrical, 4–15(–17) × (2–)3–4(–
5) μm, 0–2-septate, not constricted at septa, subhyaline to pale
olivaceous brown or brown, smooth, walls unthickened or almost
so, hila protuberant, thickened, somewhat darkened-refractive;
microcyclic conidiogenesis occurring.
Notes: True Cladosporium species, but its status is not yet clear
since all collections were very sparsely fruiting. In the duplicates
of Roumeguère, Fungi Sel. Gall. Exs. 3292 at B (B 700006190
and 700006191) only sterile stromata have been observed.
Morphologically C. asteromatoides resembles C. minusculum
but the latter species deviates in having somewhat wider, usually
minutely verruculose or verruculose conidia. Additional collections
are urgently needed to clarify the status of C. asteromatoides.
atriplicis Massee & Rodway, in herb. (?).
baccae Verwoerd & Dippen., S. African J. Sci. 27: 327. 1930.
Type: South Africa, Stellenbosch, on fruits of Vitis vinifera
(Vitaceae), B.J. Dippenaar, No. 392 in herbarium of Len Verwoerd
at Stellenbosch.
Original diagnosis (Verwoerd & Dippenaar 1930): At irst punctiform
and covered by the epidermis, erumpent, then developing a
somewhat raised, olivaceous, circular, velvety spot in the circular
infected area through conluence of the acervuli; conidiophores
recumbent, somewhat lexuous, very rarely branched, olive
coloured, darker in mass, irregularly septate, not constricted at the
septa, not nodulose, up to 128 μm long and 6.5 μm thick, slightly
swollen at the base; conidia very variable in shape and size,
spherical, subspherical, ovate, clavate, cylindrical or elongate, 1to 2-septate, not or very seldom slightly constricted at the septa,
1-celled: 12–23 × 4.8–7.5 μm, 2-celled: 9.6–17.6 × 6.5–7.0 μm,
3-celled: 25.6 × 6.5 μm, with round, blunt or slightly pointed ends,
smooth, light brown with darker walls, produced terminally.
Notes: Type material could not be traced in South Africa and is
probably not preserved.
banaticum Săvul., Bul. Sti. Acad. Republ. Populare Române
3(2): 227. 1951.
Type: Romania, Severin, Moldova Nouă, on dry leaves of Dianthus
kitaibelii (= Dianthus petraeus subsp. petraeus) (Caryophyllaceae),
11 Jul. 1948.
Original diagnosis (Săvulescu 1951): Caespitulis minutis,
punctiformibus, epi- vel hypophyllis, nervisequiis, nigricantibus,
e stromate vel hypostromate cellulose oriundis; conidiophoris
caespitosis, 8–20 in quoque caespitulo, 50–120 × 3–6 μm
simplicibus, erectis vel sublexuosis, non torulosis, continuis vel
1-septatis, fusco-bruneis apice dilutioribus, obtusiusculis; conidiis
ovoideis vel ellipsoideis 6–10 × 4–6 μm solitariis, primum continuis
subhyalinis, dein 1-septatis, palide violaceis.
Notes: Type material was not available.
bignoniae Schwein., Trans. Amer. Philos. Soc., N.S., 4(2):
277. 1832.
Syntypes: USA, Carolina, Pennsylvania, Bethlehem, on legumes of
Campsis radicans (= Bignonia radicans) (Bignoniaceae), No. 2600
(PH 01020422, 01020423).
Original diagnosis (Schweinitz 1832): C. acervulis sparsis,
minutis, frequentibus, sphaeriaeformibus, nigro-olivaceis. Floccis
breviusculis cum sporidiis concoloribus.
Lit.: Saccardo (1886: 353), Gonzáles-Fragoso (1927: 206),
Schubert & Braun (2004: 297–298).
Notes: Status unclear, type material too meagre to be identiied.
Specimen: Australia, Tasmania, on Atriplex cinerea (Amaranthaceae).
www.studiesinmycology.org
341
BenSch et al.
boenninghauseniae Togashi & Katsuki, Kyushu Agric. Res.
8: 84. 1951.
Type: Japan, Kyushu, Pref. Fukuoka, Mizu-nashi, Ito-mura, on
leaves of Boenninghausenia albilora (Rutaceae), 15 Oct. 1950, S.
Katsuki.
Ill.: Katsuki (1951: 84, ig. 1).
Original diagnosis (Katsuki 1951): Maculis amphigenis, sparsis vel
laxe gregariis, rotundatis vel subrotundatis 3–10 mm diametris,
supra griseo-brunneis, infra brunneis vel nigro-brunneis; caespitulis
hypophyllis, effusis, brunneis; conidiophoris erectis vel lexuosis,
ascendentibus, cylindraceis, simplicibus, 1–3 septatis, olivaceobrunneis, 70–105 × 5.0–7.5 μm; conidiis oblongis, cylindraceis vel
fusiformibus, utrimque rotundatis, rectis, non vel leniter constrictis,
1–3 septatis, raro non-septatis, olivaceo-brunneis, 12.5–30 × 5–6
μm.
brachormium Berk. & Broome, Ann. Mag. Nat. Hist., Ser. 2,
7: 99. 1851.
Type: On leaves of Fumaria oficinalis (Papaveraceae), King’s
Cliffe.
Lit.: Cooke (1871: 584), Saccardo (1886: 363).
Original diagnosis (Saccardo 1886): Effusum tenue, griseum;
hyphis erectis, lexuosis, sursum nodulosis; conidiis ellipsoideis
oblongis, breviter concatenatis, terminalibus.
Notes: Since the conidiophores were described as nodulose and
conidia as being ellipsoid-oblong, this species is maybe conspeciic
with C. herbarum, but the diagnosis is too sparse for a inal
conclusion.
brassicicola Sawada, Special Publ. Coll. Agric. Natl. Taiwan
Univ. 8: 195. 1959, nom. inval.
Type: Taiwan, Pref. Taichung, Taichung, on leaves of Brassica
juncea (Brassicaceae), 17 Feb. 1913, K. Sawada.
Ill.: Sawada (1959: pl. 3, igs 4–5).
Original diagnosis (Sawada 1959): Lesions on leaves, broadly
spreading, 30–50 mm, yellowish brown; conidiophores
amphigenous, single or more or less fasciculate, not branched,
2–4-septate, yellowish brown, 55–104 × 5–7 μm; conidia catenulate,
hyaline or yellowish brown, elliptical or short cylindrical, rounded at
both ends or truncate at base, 0–2-septate, 7–26 × 3–5 μm.
Notes: Cladosporium brassicicola was invalidly published since the
author failed to provide a Latin diagnosis. Cladosporium brassicae,
known from North America on Brassica oleracea, is distinguished
from the latter taxon by its coarsely verrucose or echinulate, much
wider conidia, 10–14 μm (David 1997).
brevicatenulatum Rebr. & Sizova, Novosti Sist. Nizsh. Rast.
15: 137. 1978, as “brevi-catenulatum”.
Type: Russia, Yaroslavskaya Oblast’, Rostov, isolated from ancient
cloth, 1973, T.P. Sizova.
Ill.: Rebrikova & Sizova (1978: 138, igs a–b).
Original diagnosis (Rebrikova & Sizova 1978): Coloniae in
medio agarisato Czapeki saccharoso 30 % addito ad diem
decimam 1.4–1.7 cm in diam., marginibus inaequalibus, substrato
subimmersae, ad centrum vix elevatae, puberulae, margine atroolivaceae (e4), centro nigrescentes (a2), reverse atrato (a2).
Exsudatus hyalinus, minute guttulatus centro coloniae praecipue
congestus. Conidiophora brunnescentia, numerosa, a substrato
et hyphis aeries oriunda, apice vix ramose, saepe septate, ad
septa subinlata, 190–210 × 4–5 μm. Conidia in catenulas breves
per unum-tria congesta, unicellularia (10–14 × 6–8 μm), interdum
342
bicellularia (14–20 × 6–8 μm), valde aculeate, atro-brunnea,
cylindrical, brevissime catenulate; in mediis ad sporulationem
stimulandam destinatis (agaro Raistricki, agaro aquoso)
metaconidia copiosissima, microconidia vero subnulla. Coloniae
in agaro musti maltati ad diem decimam 2.2–2.5 cm in diam.,
plicatulae, puberulo-velutinae, margine crescenti aequali, substrato
fere immerse, centro vix excavatae, margine atro-olivaceae (e4),
medio atratae (a2); exsudatus hyalinus, minute guttulatus, centro
coloniae praecique congestus.
Notes: Rebricova & Sizova (1978) mention type material as “Cultura
typica No. 10P in Laboratorio Centrali”. Based on description and
illustration this species probably belongs to C. herbarum s. lat.
brevicompactum Pidopl. & Deniak, Mikrobiol. Zhurn. 5(2):
186, 194. 1938, as “brevi-compactum”, nom. inval.
Type: Isolated from soil.
Lit.: Pidoplichko (1953: 271).
Ill.: Pidoplichko & Deniak (1938: 186, ig. 3).
Notes: Neither in Mikrobiol. Zhurn. (1938) nor in Pidoplichko (1953)
a Latin diagnosis is given.
brevicompactum var. tabacinum Pidopl. & Deniak, in
Pidoplichko, Gribnaya Flora Grubykh Kormov: 272. 1953,
nom. inval.
Type: Ukraine, isolated from fermented tobacco leaves.
Ill.: Pidoplichko (1953: 271, ig. 72).
calcareum Beeli, Bull. Soc. Roy. Bot. Belgique 56: 68. 1924.
Type: Belgium, Brussels, on lime-coated wall and wood in a cellar.
Lit.: Saccardo (1972: 1337).
Ill.: Beeli (1923: tab. 4, ig. 14).
Original diagnosis (Saccardo 1972): Maculis violaceis; mycelio
repenti brunneo septate; hyphis fertilibus adscendentibus septatis
simplicibus, 50–70 × 4 μm; conidiis ellipsoideis 1-septatis brunneis
levibus, 10 × 3–3.5 μm.
Notes: Type material could not be traced.
caricinum C.F. Zhang & P.K. Chi, Guangdong Guoshu
Zhenjun Binghai Zhi.: 54. 2000, nom. inval. (ICBN Art. 37.6).
Type: China, Guangzhou, on living leaves and fruits of Carica
papaya (Caricaceae), 1993, C.F. Zhang, No. 01371.
Ill.: Zhang & Chi (l.c.: 54, ig. 41).
Original diagnosis (Zhang & Chi, l.c.): Maculae saepe hypophyllae,
primo albidae tandem lavo-albidae, orbiculares vel ellipsodeae,
convexae, 1.7–3.3 μm diam. Fructis morbidis vivis. Cultura
in malt-extracto agaro (25°C, 7 dies): Coloniae 3.8 cm diam.,
effusae, velutinae, griseo-brunneae vel olivaceo-brunneae, dorsale
atroviridis. Conidiophora solitaria vel fasciculata, fusco-brunnea,
lexuosa, nodosa, 39–183 × 3.0–6.8 μm, laevia, septata. Conidia
saepe 0-septata, interdum 1–2-septata, catenata, orbicularia,
ellipsoidea vel cylindrica, dense verrucosa, subhyalina vel pallide
olivaceo-brunnea, 4–16.2 × 2.5–5.0 μm vulgo 5–9 × 2.5–4.2 μm.
Notes: The name C. caricinum is not validly published since there is
no indication where the type material has been deposited.
chrysanthemi Pidopl. & Deniak, in Pidoplichko, Gribnaya
Flora Grubykh Kormov: 272. 1953, nom. inval.
Type: Ukraine, on fallen petals of chrysanthemum.
Ill.: Pidoplichko (1953: 273, ig. 74).
Notes: Type material probably not preserved.
the genuS Cladosporium
chrysanthemi A.K. Das, Indian Phytopathol. 56(2): 164. 2003.
Holotype: India, Bengal, Rahara, North 24 Parganas, on living
leaves of Chrysanthemum sp. (Asteraceae), 17 Mar. 1985, A.K.
Das, HCIO No. 44743 (IMI 295539). Isotype: PCC 3719.
Ill.: Das (2003: 164, ig. 1).
Original diagnosis (Das 2003): Maculae amphigenae, distinctae,
numerosae, subcirculares vel irregulares, interdum aggregatae,
griseo vel griseo brunneae, interdum margin griseo cinctae, 1–3.5
μm latae. Caespituli hypophylli, brunneae. Mycelium immersum et
supericiale, laevis. Stroma parvum, atro-brunneae. Conidiophora
solitaria vel fasciculata, 3–10 infasciculo, recta vel curvata, pallide
brunneae, simplicia vel ramose, interdum inlatae, terminalis
et intercalaris, crasse tunicate, laevia, pluriseptate, interdum
geniculate, conidialibus nemerosis praedita, cicatrices conidiales
distincto, apice subacute vel obtuse, 3.5–4.5 μm (6.5 μm inlatae)
× 16.5–82 μm (raro 180 μm), raro curvata, cylindrica, laevia vel
verruculose, 3-septata, 10–36 × 3.5–5 μm.
circaeae Y. Qin & Z.Y. Zhang, Mycosystema 18(2): 135. 1999.
Holotype: China, Jiangxi, Lushan, on living leaves of Circaea
mollis (Onagraceae), 5 Oct. 1980, J.Y. Li & T.Y. Zhang, No. 41440
(MHYAU 03953).
Ill.: Qin & Zhang (1999: 135, ig. 1), Zhang et al. (2003: 69, ig. 38;
pl. 13, ig. 4).
Original diagnosis (Qin & Zhang 1999): Maculae amphigenae,
ellipsoideae, rubro-brunneae, centro sub-orbiculares griseae, 6
mm diam. Conidiophora solitaria, erecta, simplicia, septata, apice
sympodialiter prolifera, atro-brunnea, prope apicem pallescentia,
46–77 × 3.4–5.1 μm. Ramoconidia continua, pallide brunnea, apice
inlata denticulata, cicatrices evidentes, 8.2–12.9 × 2.6–2.8 μm.
Conidia catenata, clavata vel soleiformia, bicellularia, raro simplicia
fusiformia, pallide brunnea, basi vel utrinque cicatricosa, 1-septata,
5.7–15.9 × 2.6–3.1 μm.
citri Massee, Text book Pl. Diseas. 1899: 310. 1899.
Type: USA, Florida and Louisiana, on leaves and fruits of Citrus
medica (=×limon) (Rutaceae) (K ?).
Lit.: Saccardo (1913a: 1367), Jenkins (1925).
Original diagnosis (Saccardo 1913a): Conidiophoris caespitulosis,
erectis, ramosis, septatis, brunneis, 30–75 × 2–4 μm; conidiis
fusoideis, obscuris, pro more continuis, interdum 1–3-septatis, 8–9
× 2.5–4 μm.
Notes: Massee gave no specimen or herbarium designations, nor
a description, but on p. 311 refers to “Bull. Torrey Bot. Club 13:
181”. The only reference in this article to a named fungus is that
to a Fusarium on page 182, but on the same page “oblong, oval
one-celled conidia” are mentioned. On page 311 Massee also
cites “USDA Bull. No. 8”, which refers to the above article from
“Bull. Torrey Bot. Club” and other literature. The USDA Bulletin
attributes the disease of Citrus to a Cladosporium, for which a short
description is rendered without specifying specimens or herbaria.
Fawcett (1936: 535) wrote on the identity of C. citri that the fungus
is now regarded as a Sphaceloma. The name Cladosporium citri,
based on descriptions given by Scribner and Swingle & Weber,
applies to the fungus observed by Swingle & Weber on the old
lesions (Jenkins 1925). The pathogen isolated by Fawcett and
reported as C. citri is described as a new species, Sphaceloma
fawcettii Jenkins.
clavatum Schwabe, Fl. anhalt. 2: 349. 1839.
Type: Germany, on dry wood of Quercus sp. (Fagaceae).
www.studiesinmycology.org
Lit.: Saccardo (1913a: 1369).
Ill.: Schwabe (1839: tab. 6, ig. 13).
Original diagnosis (Schwabe 1839): Filis nigro-viridibus pellucidis,
sporis obovato-clavatis. Fila inaequalia lexuosa valde torosa
subramulosa oblique septate; articulis plerumque gibbosis. Sporae
minors concolores, 1–3-septatae.
Notes: Type material not found, probably not preserved. A collection
in the herbarium of Preuss (Germany, on wood of oak, 1844, Preuss
274, B 700006216) proved to be C. herbarum.
clemensiae P.W. Graff, Philipp. J. Sci. 9: 40. 1914.
Type: Guam, Agaña, on leaves of Eragrostis amabilis (= E. tenella)
(Poaceae), 27 Nov. 1910, M.S. Clemens.
Original diagnosis (Merrill 1914): Hypophyllis, caespitulis
erumpentibus, fuscis; hyphis ramosis, lexuosis, septatis;
hyphis fertilibus sparsis, erectis, septatis, fuscis, simplicibus,
100–150 × 4–5.5 μm; conidiis concoloribus, ellipticis oblongis
subcylindraceisve, continuis dein 1–3-septatis, leniter constrictis,
23–35 × 7.5–11.5 μm.
comesii Carbone, Atti Ist. Bot. Univ. Pavia, Ser. 2, 14: 322.
1914.
Type: Italy, Pavia, isolated from sausage [“in botulis (Salame
crudo)”].
Lit.: Saccardo (1931: 799).
Original diagnosis (Carbone 1914): Effusum, atre viridi-olivaceum.
– Razza 1. – Hyphis fertilibus cespitosis, erectis, quandoquidem
leviter tortuosis et apud apicem denticulatis, septatis, simplicibus
vel apud apicem ramosis, brunneis, 60–200 × 3–5 μm; conidiis in
hyphis et ramis acropleurogenis, continuis (rarissime 1-septatis),
dilute brunneis, rotundatis vel ovalibus vel ellipticis, 4–10 × 2–4
μm. – Razza 2. – Hyphis fertilibus fasciculatis, saepe curvatis vel
undosis et tortuosis, brunneis, quandoquidem apud apicem breviter
ramosis, septatis, 50–100 × 4 μm; conidiis in hyphis et in ramis
acropleurogenis, rotundatis vel ovalibus vel ellipticis, continuis,
dilute brunneis, breviter catenulatis, 4–12 × 2–4 μm.
Notes: Type material could not be found, and has possibly not been
maintained.
compactiusculum Sacc. & P. Syd., Syll. fung. 14: 1082. 1899.
Type: Ceylon, on twigs of Sterculia foetida (Malvaceae) and
?Frusinalia (Combretaceae).
≡ Cladosporium subcompactum Roum. & P. Karst., Rev. Mycol.
(Toulouse) 12: 80. 1890, nom. illeg. homonym, non C. subcompactum
Sacc., 1886.
≡ Cladosporium zeylanicum Sacc. & Trotter, Syll. fung. 22: 1371. 1913,
nom. superl.
Original diagnosis (Karsten et al. 1890): Caespituli supericiales,
tomentosi, compacti, suborbiculares, atri, 1–5 μm lati. Hyphae
dense aggregatae et intricatae, ramosae, articulatae, fuligeneohyalinae vel dilute fuligineo-olivaceae, 3–6 μm crassae. Conidia
ovoidea vel ellipsoidea, 1-septata, ad septum non constricta, e
hyalino fuligineo-fusca, impellucida vel semipellucida, longit. 20–28
μm, crassit. 12–16 μm.
Notes: The original material from PC is deposited under
“Cladosporium subcompactum B. et C.” (on Sterculia foetida,
Ceylon). Status remains unclear.
compactum Sacc. → subcompactum Sacc.
compactum *punctatum Sacc. → punctatum Sacc.
343
BenSch et al.
corchori Z.Y. Zhang & T. Zhang, in Zhang, Liu, Wei & He,
Plant Diseases and Their Control: 103. 1998.
Holotype: China, Shaanxi, Yizhan, on living stems of Corchorus
capsularis (Malvaceae), 20 Aug. 1973, J.Y. Li & T.Y. Zhang, No.
0412 (MHYAU 03955).
Ill.: Zhang et al. (1998c: 104, ig. 1), Zhang et al. (2003: 79, ig. 43;
pl. 11, ig. 3).
Original diagnosis (Zhang et al. 1998c): Caulinus blightus, 40 mm
longus, coloniae punctiformae, interdum conluentia, velutinae,
fuscae. Mycelium immersum. Conidiophora solitaria, erecta,
simplicia, septata, brunnea, prope apicem pallescenta, 40 × 40 μm,
parte fertili sympodialiter prolifera leviter geniculata denticulata.
Ramoconidia continua vel raro 1-septata, pallide brunnea, apice
± inlata denticulata, 7.7–19.3 × 2.8–3.9 μm. Conidia catenata,
continua, fusiformis, laevia, pallide brunnea, utrinque cicatricis
fuscis, 4.4–8.0 × 2.6–4.6 μm.
Notes: This species is reported to cause stem blight on Corchorus
capsularis. Induced symptoms are shown in Zhang et al. (2003, pl.
XI, ig. 3).
corynitrichum Ellis & Everh., in Millspaugh, West Virginia
Geol. Surv., Ser. A, 5: 36. 1913, nom. nud.
Type: USA, West Virginia, Fayette Co., on dead fallen leaves of
Magnolia fraseri (Magnoliaceae), 29 Nov. 1895, L.W. Nuttall (NY,
WIS).
Notes: It is a mixed collection of various hyphomycetes, including
numerous large, medium to dark brown subcylindrical to cylindrical,
verruculose, 3-septate conidia of C. herbarum and conidiophores
with typical swellings as well as small smooth conidia and smaller
conidiophores with small loci. Ascomycetous stromata and
immature ascomata are also present.
cyrtomii Z.Y. Zhang, H.H. Peng & H. Zhang, Mycosystema
17(1): 4. 1998.
Type: China, Prov. Yunnan, Gejiu, on living leaves of Cyrtomium
caryotideum (Dryopteridaceae), 9 Dec. 1994, Wang Ying-Xiang &
Li Mao-Lan (MHYAU 04048).
Ill.: Zhang et al. (1998a: 4, ig. 1), Zhang et al. (2003: 84, ig. 48;
pl. 11, ig. 4).
Original diagnosis (Zhang et al. 1998a): Maculis amphigenis,
distinctis, interdum conluentibus, rotundis, atro-brunneis,
marginibus fuligineis prominulis, 2–4 mm diam., postea interdum
perforatis, epiphyllis velutinis, atro-viridibus, hypophyllis fulvis.
Conidiophoris fasciculatis, rectis vel leniter lexuosis, septatis,
atro-brunneis, ad apicem pallide brunneis, 167–321 × 2.6–4.6
μm. Cellulis conidiogenis in conidiophoris coalitis, sympodialibus,
cicatricibus conspicuis. Ramoconidiis 0–1 septatis, ad apicem
denticulatis, cicatricibus conspicuis, 7.7–17.9 × 2.6–5.1 μm.
Conidiis subellipsoideis, fusiformibus, longe ellipsoideis vel
cylindricis, continuis, laevis, atro-olivaceis, 2.6–12.8 × 2.6–5.1 μm.
delectum f. ailanthi-glandulosae Thüm., Mycoth. Univ., Cent.
XVII, No. 1666. 1880, nom. nud.
Syntypes: USA, South Carolina, Aiken, on leaves of Ailanthus
altissima (= A. glandulosa) (Simaroubaceae), H.W. Ravenel,
Thümen, Mycoth. Univ. 1666 (e.g., HAL, NY).
Notes: Material too sparse for a inal conclusion about its status.
densum Sacc., Bull. Orto Bot. Regia Univ. Napoli 6: 71. 1921.
Type: Italy, Salerno, Scafati, on dead stems of Ricinus communis
(Euphorbiaceae), A. Trotter.
344
Lit.: Saccardo (1931: 791).
Original diagnosis (Saccardo 1921): Maculas atras, velutinas, in
caule longitrorsum elongatas, saepe rhomboideas 0.5–1.5 cm.
long. formans; conidiophoris dense lateque parallele stipatis,
non caespitulosis, iliformibus, subrectis, simper indivisis, 80–
120 × 4, aequalibus (non nodosis), parce septatis, totis dilute
olivaceo-fuligineis, ad apicem obtusiusculum vix dilutioribus,
ex hypostromate brunneo, laxe celluloso orientibus; conidiis
versiformibus, typice oblongis, 1-septatis, non constrictis, 10–15
× 4–5, raro magis elongatis 3–4-septatis, 18–22 × 5 (junioribus
breviter ovoideis, continuis).
Notes: Type material not preserved in Saccardo’s herbarium at
PAD. Status remains unclear.
desmodicola A.K. Das, Indian Phytopathol. 56(2): 165. 2003.
Holotype: India, Bengal, Howrah, Bagnan, on living leaves of
Desmodium gangeticum (Fabaceae), 4 May 1985, A.K. Das, HCIO
No. 44742 (IMI 296439). Isotype: PCC 3754.
Ill.: Das (2003: 165, ig. 2).
Original diagnosis (Das 2003): Maculae amphigenae, venaslimitatae, angulares vel irregulars, pallide brunneae vel griseobrunneae, 3–12 μm latae. Caespituli amphigenae, plerumque
epiphyllae, leniter effusae, pallide olivacea-brunneae vel fuscae.
Mycelium partim immersum, partim supericiale, laevis. Stroma
nullum. Conidiophora amphigenae, singularia vel fasciculata (3–5
infasciculo), recta vel lexuosa, apicem versus, inlatae, pallide vel
pallide olivacea brunneae, laevis, crasse tunicate, pluriseptata
(usque ad 15), cicatrices conidials distinctae, apicem obconico
rotundata vel rotundata, intercalares inlatae 5–9 μm lata, 66–429
μm (560 μm) × 4–7 μm. Conidia solitaria vel catenata (usque ad
3), elliptica vel subsphaerica, olivacea, interdum pallide olivacea
brunneae, 0–2 septata, laevia vel minute echinulate, 4.5–6.2 ×
2.5–7.5 μm.
Notes: The type material from IMI could not be traced.
desmotrichum Desm., Ann. Sci. Nat. Bot., Sér. 3, 16: 297.
1851, as “desmitrichum”.
Type: France, on dry leaves of Fraxinus ornus (Oleaceae).
Lit.: Saccardo (1886: 360), Ferraris (1912: 346), Oudemans (1923).
Original diagnosis (Desmazières 1851): C. amphigenum, nigrum.
Floccis fasciculatis, rigidis, simplicibus, divergentibus, septatis,
fusco-brunneis, apice subobtusis, hyalinis; fasciculis aggregatis.
Sporulis ovoideis vel ellipsoideis, hyalinis, simplicibus vel didymis.
Notes: Type material not seen. Most collections deposited as C.
desmotrichum belong to C. herbarum (e.g., on Fraxinus sp., ex
herb. Bresadola, Nov. 1922, BPI 426449).
dianellicola Y. Cui & Z.Y. Zhang, Mycosystema 20(4): 470.
2001.
≡ Cladosporium dianellicola Z.Y. Zhang & Y. Cui, Flora Fungorum
Sinicorum, Vol. 14: 88. 2003, nom. superl.
Holotype: China, Zhejiang Prov., Hangzhou, on living leaves of
Dianella ensifolia (Xanthorrhoeaceae), 2 Nov. 1980, J.Y. Li & T.Y.
Zhang (MHYAU 03922).
Ill.: He & Zhang (2001: 470, ig. 2), Zhang et al. (2003: 88, ig. 52).
Original diagnosis (He & Zhang 2001): Maculis purpureobrunneis, in centro griseis, 1.5–3.5 cm longis. Conidiophoris
brunneis, 1–3-septatis, 82.2–187.6 × 2.6–4.6 μm. Ramoconidiis
0–2-septatis, 7.5–18.5 × 4.6–5.1 μm. Conidiis ellipsoideis vel
sub-globosis, continuis, pallide brunneis, 3.1–10.3 × 3.1–4.1
μm.
the genuS Cladosporium
Notes: Type material was not available. Heterosporium dianellae
Sawada (nom. inval.), described on leaves of Dianella ensifolia
from Taiwan, proved to be a true member of the genus Stenella
[= Stenella dianellae (Sawada & Katsuki) Goh & W.H. Hsieh]
(David 1997, Crous & Braun 2003), now assigned to Zasmidium
(see Heterosporium dianellae), and possesses inely verruculose,
longer conidia, 20–45 × 2.5–3.5 μm.
digitalicola Z.Y. Zhang, T. Zhang & W.Q. Pu, Mycosystema
17(3): 195. 1998.
Holotype: China, Prov. Yunnan, Kunming, on living lowers of
Digitalis purpurea (Plantaginaceae), 25 Jun. 1990, Li Hua (MHYAU
03934).
Ill.: Zhang et al. (1998d: 196, ig. 1), Zhang et al. (2003: 90, ig. 53).
Original diagnosis (Zhang et al. 1998d): Maculis amphigenis vel
petalicolis, circularibus, brunneis vel atrobrunneis, 0.5 mm diam.
Conidiophoris singularibus vel usque ad 36 fasciculatis, rectis vel
geniculatis, 3–10 septatis, ad apicem 3–5 cicatricibus conspicuis,
nodis 5.2–9.1 μm crassis, atrobrunneis, ad apicem brunner is vel
hyalinis, 45–502 × 4.0–7.9 μm. Ramoconidiis longe cylindricis,
ovoideis vel limoniformibus, 0–1 septatis, cicatricibus 3–5 hyalinis
vel pallide brunneis, 5.2–20.8 × 2.6–7.8 μm. Cellulis in conidiogenis
in conidiophoris coalitis, sympodialibus. Conidiis longe ellipsoideis,
ovoideis, limoniformibus vel cylindricis, 0–4 septatis, levis,
catenulatis, cicatricibus conspicuis, hyalinis vel pallide brunneis,
3.9–15.6 × 2.6–11.8 μm.
Notes: Type material was not available.
dufourii Brond., Arch. Fl. 1: 60, 1854.
Type: France, on decaying fruits of cucurbits (Cucurbitaceae),
autumn and winter.
Lit.: Saccardo (1886: 370).
Original diagnosis (Brondeau 1854): Dense caespitosum,
ilamentis ramosis, geniculatis, septatis, olivaceis, e macula
circulari atrocaerulea orientibus. Sporidiis rotundis vel oblongis,
saepe didymis.
Notes: Types of Louis de Brondeau are at TL [according to Staleu
& Mennega (1995)], but no material seen.
edgeworthiae H. Zhang & Z.Y. Zhang, Mycosystema 17(4):
305. 1998.
Holotype: China, Jiangxi, Lushan, on living leaves of Edgeworthia
gardneri (= E. chrysantha) (Thymelaeaceae), 15 Oct. 1980, J.Y. Li
& T.Y. Zhang (MHYAU 03957).
Ill.: Zhang & Zhang (1998a: 305, ig. 2), Zhang et al. (2003: 92, ig.
55).
Original diagnosis (Zhang & Zhang 1998a): Maculis amphigenis
distinctis vel conluentibus, griseo-brunneis, margine fuligeneis
prominulis, atro-brunneis, lave holonatis, 6.0 × 1.0 cm. Conidiophoris
solitariis, erectis, simplicibus, septatis, apice sympodialiter
elongatis, pallide brunneis, prope apicem pallescentibus, 54–
183 × 2.8–3.9 μm. Ramoconidiis 0–1-septatis, laevibus, pallide
brunneis, ad apicem inlatis denticulatis, cicatricibus conspicuis,
fuscis, 7.5–23.0 × 4.1–5.1 μm. Conidiis catenatis, fusiformibus,
continuis, laevibus, pallide brunneis, basi vel utrinque cicatricibus
protudentibus praeditis, 3.9–10.3 × 2.8–4.6 μm.
elaeagnus Gapon., Uzbekistan Biol. Zurn. (1964).
Type: Uzbekistan, Bukharskaya
(Elaeagnaceae).
Lit.: Sagdullaeva et al. (1990: 47).
www.studiesinmycology.org
Oblast’,
on
Elaeagnus
Notes: This species is listed in Sagdullaeva et al. (1990) without
giving volume and page numbers. Neither the original diagnosis
nor type material were available.
elegantulum Pidopl. & Deniak, Mikrobiol. Zhurn. 5(2): 182,
193. 1938, nom. inval.
Type: Ukraine, from a rotting fruit of Malus sp. (Rosaceae) and
from meat.
Lit.: Pidoplichko (1953: 270).
Ill.: Pidoplichko & Deniak (1938: 182, ig. 1).
Notes: Type material not seen, probably not preserved. Status
remains unclear. The diagnosis is given in Russian.
epibryum Cooke & Massee, in Cooke, Grevillea 17(84): 76.
1889.
Type: USA, on capsules of various mosses, E.G. Britton (K).
Lit.: Saccardo (1892: 605).
Original diagnosis (Cooke 1889): Caespitulis minutissimis, atris.
Hyphis simplicibus, brevibus, lexuosis, sepatis, olivaceis, superne
pallidioribus; conidiis ellipticis, utrinque rotundatis, uniseptatis,
medio constrictis, pallide fuscis, hyalinis, 18–20 × 10–12 μm.
Notes: The status of this species is unclear. Type material is
preserved at Kew (K), but too scant to be re-examined, so a loan of
this material was not possible.
epiphyllum f. castaneae-sativae Thüm., Herb. Mycol. Oecon.
Fasc. 10, No. 469. 1877, nom. nud.
Type: On dead leaves of Castanea sativa (Fagaceae), Krain,
Laibach, 1876, W. Voss, Thümen, Herb. Mycol. Oecon. 469 (e.g.,
M-0057492).
Notes: This is a member of the C. herbarum complex, but the exact
identity is unclear.
eucalypti Tassi, Bull. Lab. Orto Bot. Reale Univ. Siena 3(1):
20. 1900.
Type: Italy, Siena, botanical garden, on frozen leaves of Eucalyptus
globulus (Myrtaceae).
Lit.: Saccardo (1902: 1057), Lindau (1907: 827), Ferraris (1912:
342), Gonzáles-Fragoso (1927: 202).
Ill.: Gonzáles-Fragoso (1927: 202, ig. 45).
Original diagnosis (Saccardo 1902): Caespitulis gregariis, maculas
orbiculares atro-olivaceas usque 4 mm diam. formantibus; hyphis
intricato-ramosis, subfasciculatis, suberectis, quandoque prope
basim leniter nodulosis, olivaceis, 8–10 μm latis; conidiis ovoideis
v. oblongis, 1–3-septatis, non constrictis, levibus, 25–30 × 8–10
μm, olivaceis.
Notes: Type material is not preserved in Tassi’s herbarium at herb.
SIENA. A collection on dead leaves of Eucalyptus globulus from
Spain, recorded and illustrated by Gonzáles-Fragoso (1927) has
been examined (MA 06397) and proved to be C. cf. herbarum
(conidia typical for this species, but conidiophores less distinctly
nodulose as in typical samples). A second collection from Spain
on brown lesions on Eucalyptus viminea (MA 07502) is quite
distinct. The conidiophores are very long, up to 350 μm, iliform,
non-geniculate, only with few terminal scars, and the conidia are
ellipsoid-ovoid, subcylindrical, 3–18 × 2–5 μm, 0–1-septate, pale
olivaceous. It can be referred to as C. cf. cladosporioides. However,
the conidia are consistently verruculose.
euphorbiae Politis, Pragmat. Akad. Athen 4: 39. 1935.
Type: Greece, Attica, near Athina, on Euphorbia sibthorpii
345
BenSch et al.
(Euphorbiaceae).
Lit.: Saccardo (1972: 1337).
Original diagnosis (Politis 1935): Caespitulis parvis, nigricantibus,
dispersis, v. gregariis; conidiophoris in fasciculos densos, erectos,
atro-olivaceos confertis, septatis, 40–70 × 5–6 μm; conidiis
acrogenis lavido-brunneis, ovoideis, v. oblongis, continuis v.
uniseptatis, 5–11 × 4–5 μm.
Notes: Type material not found. Status remains unclear.
farnetianum Sacc., Syll. fung. 22: 1366. 1913.
Type: Italy, Sicily, on fruits of Citrus medica (=C. ×limon) (Rutaceae).
≡ Cladosporium citri Briosi & Farneti, Atti Ist. Bot. Univ. Pavia, Ser. 2, 10:
19. 1907, nom. illeg., homonym, non C. citri Massee, 1899.
≡ Kurosawaia citri Hara, List of Japanese Fungi, ed. 4: 172. 1954, nom.
nov., as “(Briosi & Farneti) Hara”.
Lit.: Ferraris (1914: 884).
Original diagnosis (Saccardo 1913a): Hyphis erectis v.
adscendentibus, rectis v. lexuosis, simplicibus, septatis, apice
plerumque oblique obtusatis, brunneis v. pallide fuscis, solitariis v. 2–4
caespitosis, 50–70 × 3–4.5 μm, rarius 110 × 5 μm, e pseudostromate
immerso orientibus; conidiis ellipsoideis v. oblongis, simplicibus v.
didymis, pallide fuscis, minutis, 5–11 × 4 μm.
Notes: See comments on C. citri Massee above.
fasciculare Fr., Syst. mycol. 3(2): 370. 1832.
Type: On herbal stems.
≡ Dematium articulatum Pers., Neues Mag. Bot. 1: 121. 1794.
= Helminthosporium vesicarium Wallr., Fl. crypt. Germ. 2: 166. 1833.
Lit.: Cooke (1871: 583), Saccardo (1886: 367), Lindau (1907: 817),
Ferraris (1912: 339), Oudemans (1919–1924), Gonzáles-Fragoso
(1927: 207).
Ill.: Persoon (1794: tab. 4, ig. 2).
Original diagnosis (Fries 1832): Fasciculis minutis leviter
erumpentibus, ibris apice lexis subseptatis, sporidiis conglobatis
seriatisque concoloribus.
Notes: Type material could not be traced, probably not preserved.
Status remains unclear.
fasciculatum f. amerotrichum Traverso, Malpighia 19: 149.
1905.
Type: Italy, Prov. Di Como, Villa Stroppa in Tradate, on faded
leaves of Gladiolus sp. (Iridaceae), Sep. 1902.
Lit.: Saccardo (1906: 577), Lindau (1907: 817), Oudemans (1919).
Original diagnosis (Traverso 1905): A typo differt conidiophoris
omnino continuis necc septatis.
Notes: Traverso (1905) described it as differing from typical C.
fasciculatum in having non-septate conidiophores. Type material
could not be traced, probably not preserved.
fasciculatum var. densum Ravenel, Fungi Amer. Exs., Cent.
7, No. 602. 1882, nom. nud.
Type: USA, South Carolina, Aiken, on Euonymus japonicus
(Celastraceae), Ravenel, Fungi. Amer. Exs. 602 (e.g., BPI 426554).
Notes: A true Cladosporium. The conidiophores are 30–110
× 4–6 μm, brown, smooth, thin-walled, without any swellings,
conidiogenous loci 1.5–2 μm diam. The conidia are formed in
simple or branched chains, broadly ellipsoid to cylindrical, 10–25
× 4–7 μm, 0–2-septate, pale brown, thin-walled, smooth. However,
the material examined was too scarce for a inal conclusion.
Furthermore some verruculose, C. herbarum-like conidia have also
been observed in this material.
346
festucae Sawada, Bull. Gov. Forest Exp. Sta. 105: 95. 1958.
Type: Japan, Tohoku District, on leaves of Festuca japonica
(Poaceae), 29 Sep. 1947.
Original diagnosis (Sawada 1958): Sine maculis. Fungus
amphigenus, sparsus vel confertus, solum afixus in epidermide,
obscuri-cinereus, minutus, 0.1–0.5–0.7 mm diam. Conidiophorum
epigenum, assurgens recte vel oblique ex serpente hypha, simplex
vel 1–2 ramiicans, septum partis, apud quam conidiophorum divisum
ex hypha, locatum 18–20 μm distans adversum conidiophorum,
6–10 septatum, cinereo-fuscum, cylindricum, 235–390 × 4.5–5 μm.
Conidium catenulatum, hilum leviter exertum, brevi-ellipticum vel
longi-oblongum, 0–1 septatum, cinereum, 5–15 × 4–6 μm.
Notes: Type material could not be traced, neither in herb. PPMH
nor BPI, and is probably not preserved.
ici F. Patt., Bull. Torrey Bot. Club 27: 285. 1900.
Type: USA, Department of Agriculture, green-house, on living
leaves of Ficus parcellii (Moraceae), Jan. 1900, F.W. Patterson
(herb. of the “Division of Vegetable Physiology and Pathology, U.S.
Department of Agriculture”).
Lit.: Saccardo (1902: 1059).
Original diagnosis (Patterson 1900): Not forming spots; tufts
conspicuous, aggregated, sometimes conluent, olive green;
hyphae long, erect, slightly wavy, very rarely branched, septate,
45–250 × 4 μm; conidia light olive, terminal and lateral, more
especially borne near the tips of the hyphae, oblong continuous
ones 6–9 × 4 μm, sometimes in chains of 4, those 1–3 septate,
oblong-elliptical to cylindrical, 9–25 × 4–5 μm, somewhat thickened
at the septa and the longer ones often equilateral.
Specimens examined: Cuba, Santiago de Las Vegas, Antilles, on Ficus religiosa,
12 Feb. 1920, S. Bruner (BPI 426579); 28 Enero 1920, D.C. Brunner & B. Chias
(?) (BPI 426578).
Notes: Type material of this species could not be traced and was
not available for a re-examination. Two collections from BPI,
determinated as C. ici, showed a Passalora species well agreeing
with the species concept of Passalora urostigmatis (Henn.) Crous &
M.P.S. Câmara. The latter species deviates from the description of
C. ici given above in forming shorter and wider conidiophores and
solitary conidia so that this name was very probably misapplied.
Ficus religiosa is a new host species of Passalora urostigmatis,
until now known from Brazil, Venezuela and Florida, USA (Crous
& Câmara 1998).
forsythiae Z.Y. Zhang & T. Zhang, J. Anhui Agric. Univ. 26:
36. 1999.
Holotype: China, Henan, Zhengzhou, on living leaves of Forsythia
suspensa (Oleaceae), 20 Aug. 1992, Qing Yun (MHYAU 07030).
Ill.: Zhang et al. (1999b: 37, ig. 1), Zhang et al. (2003: 101, ig. 63).
Original diagnosis (Zhang et al. 1999b): Maculis amphigenis, apeicis
vel marginis, brunneis vel atro-brunneis, marginalibus fuligineis
longi-undulatis ornatis, spot blight usque ad 5–10 × 3–8 mm vel 35 ×
30 mm, olivaceis mucoris punctatis hypophyllis. Myceliis immersis.
Stromata praeditis Conidiophoris solitariis, erectis vel sinualatis,
simplicibus, septatis, apice denticulatis, cicatricibus conspicuis,
brunneis vel atro-brunneis, prope apicem pallescentibus, 21.0–69.0
(36.3) × 3.6–5.1 (4.4) μm. Cellulis in conidiogenis in conidiophoris
coalitis, sympodialibus. Ramoconidiis cylindricis, 0–1 septatis,
rarius 2 septatis, cicatricibus protrudentibus praeditis, brunneis,
7.7–18.0 (12.9) × 4.4–5.9 (5.2) μm. Conidiis catenatis, fusiformis,
cylindricis, ovoides, laevis, rarius echinatis, 0–1 septatis, rarius 2
septatis, pallide brunneis vel hyalinis, cicatricibus ornatis, 5.7–38.6
(10.1) × 4.4–5.1 (4.8) μm.
the genuS Cladosporium
Notes: A second collection is mentioned by Zhang et al. (1999b) on
Forsythia sp. from Liaoning, China.
glochidionis C.D. Sharma, Gadp., Firdousi, A.N. Rai & K.M.
Vyas, Indian Phytopathol. 51(2): 152. 1998.
Type: Australia, Victoria, Doncaster and elsewhere, on fruits of
Citrus medica (Rutaceae).
Lit.: Saccardo (1902: 1058).
Ill.: McAlpine (1899: Figs 19–20).
Original diagnosis (McAlpine 1899): Forming dingy-white patches
on surface of Lemon, ultimately becoming dotted over with
brownish-black spots. The discoloured portions readily peel off
in minute lakes, and the Lemon becomes scurfy in appearance.
Hyphae yellowish-green, septate, branched, regular or irregular,
in breadth 4–5.5 μm. Conidia ovate to pear-shaped, lemon-yellow,
continuous or 1-septate, not constricted at septa, pretty constant in
size, 15–17 × 8.5–9.5 μm.
Notes: Type material is not preserved in the McAlpine herbarium
(VPRI).
Holotype: India, Madhya Pradesh, Shahdol circle, Amarkantak
(south forest division), Kapil dhara, on living leaves of Glochidion
sp. (Phyllanthaceae), Jan. 1993, C.D. Sharma (S.U. Herb. No.
C.S.1). Isotype: IMI 356765.
Ill.: Sharma et al. (1998: 153, ig. 1).
Original diagnosis (Sharma et al. 1998): Maculae amphigenosae,
parvae vel magnae, aetate progrediente, se extendentes per majorum
supericiem folii, plerumque initiate ad marginateae, interdum
necroticae, brunneae infra supericiem et griseo albidus supra
supericiem, cum fuscae brunneae margin. Caespitulli amphiphyllae
plerumque hypophyllae, ad maculae limitatae, plus minusve lanatae,
brunneae atro. Mycelium hypharum, immersum vel supericiales,
angustum, laevia, ramosum, septatum, olivacea brunneae, 1.5–5
μm diametro, hyphae repentes usque 6.5 μm crassa. Stromata bene
evoluta, irregulares, pseudoparenchymatosa, obscure brunneae, 24–
70 μm diametro. Conidiophora caespitosa vel solitaria, macronematosa,
mononematosa, septata eramosa, erecta vel suberecta, recta vel
interdum lexuousa, plerumque glabra interdum verruculosa plerumque
tumoribus ad apices, olivaceo-brunneae 7–137 × 1.5–6.5 μm et usque
9.5 μm ad basim. Cellulae conidiogenosae integrateae, terminales et
intercalares, polyblasticae, sympodiales, cicatricatae, cicatricis fuscis
distinctae et crassato. Conidia simplicia, variabilis catenata vel solitaria,
arida, acropleurogenosa, subcylindrata vel obclavato-cylindrata
interdum clavato-cylindrata, doliiformia, ellipsiformia, fusiformia, ovata,
spheriformia vel subspheriformia, apices obtusa vel subacuta, bases
rotundatae, obconico-truncata vel subtruncata, usque 0–5 transversae
septata rarissime oblique septata, hilo fuscae distinctae et crassata,
interdum emenentia, conidia progenerentes progenrent tube longa
et eramosa, laevia, septata, usque 28 × 3 μm pallide vel moderate
olivacea, 2–45 × 1.5–12 μm.
Notes: Type material was not available neither from India nor IMI
(probably lost). Cladosporium glochidionis was morphologically
compared with C. spongiosum, C. diaphanum and C. buteicola but
proved to be distinct in its symptomatology, well-developed, irregular
stromata, olivaceous-brown, smooth, sometimes verruculose
conidiophores and comparatively thicker, variously shaped, light
to medium olivaceous conidia (Sharma et al. 1998). Sharma et
al. (1998) described and illustrated protuberant, darkened and
thickened conidiogenous loci and hila, so C. glochidionis possibly
belongs to Cladosporium s. str. New collections are necessary to
prove the generic afinity of this species.
fuscum Link, Sp. pl. 6(1): 40. 1824 : Fr., Syst. mycol. 3(2):
372. 1832.
gossypiicola Pidopl. & Deniak, in Pidoplichko, Gribnaya Flora
Grubykh Kormov: 273. 1953, as “gossypicola”, nom. inval.
fuligo Bonord., in herb.
Specimen: On Tilia europaea (Tiliaceae), Funk (B).
fulvum var. violaceum Voglino, Ann. Reale Accad. Agric.
Torino 55: 381. 1912, 1913.
Type: Italy, Liguria, Albenga, on leaves of Solanum lycopersicum
(= Lycopersicon esculentum) (Solanaceae).
Lit.: Saccardo (1931: 794).
Original diagnosis (Voglino 1913): Maculis lavescentibus, rotundis,
6–8–10 mm latis, numerosis; caespitulis effusis, lanosis, violaceis
vel violaceo-fuliginosis, fulvis; conidio-foris erectis, septatis, non vel
parce ramosis, apice clavatis, violaceo-fuliginosis, fulvis, 100–150
μm longis, 5–7–10 μm crassis; conidiis ellipsoideis, continuis,
1-septatis, pallide lavis, 14–20–28, rar. 40 μm longis, 8–10–12 μm
latis.
Notes: Very probably synonymous with Passalora fulva (Cooke)
U. Braun & Crous.
fumosa Preuss, in herb.
Specimen: Without detailed data, Nr. 386 (Mappe 274), herb. C.G.T. Preuss
(B 700006438).
Notes: The collection contains leaves of various host species, but
no trace of a Cladosporium has been found.
furfuraceum McAlpine, Fungus Dis. Citrus Trees Austral.: 78.
1899.
Lectotype (designated here): Germany, Berlin, ?Link (B
700006442).
Lit.: Fries (1832: 372), Saccardo (1886: 352), Lindau (1907: 805),
Ferraris (1912: 335), Oudemans (1921), Gonzáles-Fragoso (1927:
201).
Original diagnosis (Link 1824): Cl. thallo effuso, loccis dense
aggregatis fuscis apice ramosis, sporidiis globosis. Tomentum
format fuscum durum late caules plantarum investiens. Sub
microscopio composito locci dense aggregati transparentes ut
solent in hoc genere continui, apice multo magis ramosi ac in
praecedentibus luculentius septate. Saepissime locci decumbunt
a pluvial uti videtur prostrate. Sporidia hinc inde adspersa.
Notes: Hughes (1958: 751) examined and annotated the material
designated as lectotype and stated that it just represents a sterile
basidiomycetous fungus, which could be conirmed.
www.studiesinmycology.org
Type: Ukraine, on seeds of cotton (Gossypium sp., Malvaceae),
also found on damp straw.
= ? Cladosporium cladosporioides (Fresen.) G.A. de Vries
Lit.: Ho et al. (1999: 128).
Ill.: Pidoplichko (1953: 274, ig. 75), Ho et al. (1999: 130, ig. 20).
Notes: Cladosporium gossypiicola is an invalid name (type material
probably not preserved). Ho et al. (1999) examined a culture isolated
from cotton in Israel. At the CBS this culture was reclassiied as “C.
tenuissimum”, but on account of shorter conidiophores, Ho et al.
(1999) maintained C. gossypiicola as a separate species. However,
the original description as well as the culture studied by Ho et al.
(1999) are rather close to C. cladosporioides. The culture studied
by Ho et al. (1999) was included in the phylogenetic studies within
the C. cladosporioides complex and proved to be conspeciic with
C. cladosporioides (Bensch et al. 2010).
347
BenSch et al.
Zhang et al. (2003) cited collections on Bombax malabaricum
(China, Shaanxi), Gossypium hirsutum (China, Henan), and
Gossypium sp. (China, Hubei).
Notes: In Oudemans (1919), Pteris aquilina (= Pteridium aquilinum)
is mentioned as host species. See C. aquilinum. No type material
could be traced.
gossypiicola var. minor Pidopl. & Deniak, in Pidoplichko,
Gribnaya Flora Grubykh Kormov: 273. 1953, nom. inval.
helminthosporioides (Corda) Fr., Summa veg. Scand. 2: 499.
1849.
Type: Ukraine, Krim, on seeds of cotton (Gossypium sp.,
Malvaceae).
Notes: Type material unknown, status remains unclear.
graminum f. bambusae Roum., Fungi Sel. Gall. Exs., Cent.
XLII, No. 4191. 1887, nom. nud.
Syntypes: France, Dept. Haute-Garonne, Vallee du Lys, Bagnèresde-Luchon, park of casino, on leaves of Bambusa sp. (Poaceae),
Dec. 1886, Ch. Fourcade, Roumeguère, Fungi Sel. Gall. Exs. 4191
(e.g., B).
graminum f. inlorescentiae Sacc., Syll. fung. 25: 791. 1931.
Type: France, on lowers of Phalaris arundinacea (= Baldingera
arundinacea) (Poaceae), Hariot.
Original diagnosis (Saccardo 1931): Est forma caerealibus, noxia,
forte cum Sphaerella tulasnei Janez. connexa. Hyphae 4.7–5 μm
cr. e basi stromatica fasciculatae; conidia saepius bicellularia, 7–9
× 5 μm, fuliginea.
Notes: Type material not found. Status remains unclear.
graminum f. poae-pratensis Thüm., Mycoth. Univ., Cent. 5,
No. 490. 1876, nom. nud.
Syntypes: Germany, Bavaria, Bayreuth, on dead culms of Poa
pratensis (Poaceae), 1874, Thümen, Mycoth. Univ. 490 (e.g., HAL,
M-0057620).
Notes: No Cladosporium found in the original collections
examined.
grewiae Bacc., Ann. Bot. (Rome) 4: 277. 1906, as “graeviae”.
Type: Eritrea, Mai Hiryi, Pappi, on dead leaves of Grewia
(Malvaceae).
Lit.: Saccardo (1913a: 1368).
Original diagnosis (Saccardo 1913a): Caespitulis epi-hypophyllis
maculis aridis late effusis, insidentibus; hyphis erectis, simplicibus,
gregariis vel fasciculatis, septatis, 70–200 μm longis, conidiis apici
et lateri hypharum insidentibus, continuis, uniseptatis vel rarius
biseptatis, rotundis vel ovatis 6–7 μm long.
Notes: Type material could not be traced; status remains unclear.
griseo-olivaceum Pidopl. & Deniak, Mikrobiol. Zhurn. 5(2):
183, 193–194. 1938, nom. inval.
Type: Ukraine, on grains of Zea mays (Poaceae).
Lit.: Pidoplichko (1953: 271).
Ill.: Pidoplichko & Deniak (1938: 183, ig. 2).
Notes: Neither in Mikrobiol. Zhurnal (1938) nor in Pidoplichko
(1953) a Latin diagnosis is given. Type probably not preserved.
Status remains unclear.
grumosum (Pers.) Link, Sp. pl. 6(1): 42. 1824.
Type: Not speciied in the original publication.
≡ Dematium grumosum Pers., Mycol. eur. 1: 16. 1822.
Lit.: Fries (1832: 373), Corda (1837: 14), Saccardo (1886: 363),
Lindau (1907: 822).
Original diagnosis (Link 1824): Cl. acervulis densis sparsis minutis
grumosis fusco-nigricantibus.
348
Type: Czech Republic, on dead leaves of conifers.
≡ Azosma helminthosporioides Corda, Deutsch. Fl. 3(12): 35. 1831.
≡ Macrosporium helminthosporioides (Corda) Sacc. & Traverso, Syll.
fung. 20: 8. 1911.
Ill.: Corda (1831: tab. 18).
Original diagnosis (Corda 1831): A. acervulis oblongis olivaceis;
sporis cuneiformibus diophanis olivaceis, loccis simplicibus
concoloribus.
Notes: Type material could not be located in herb. PRM.
herbarum f. agaves-echeveriae Savelli, Ann. Reale Accad.
Agric. Torino 56: 113. 1914 (dated 1913).
Type: Italy, Torino, on living leaves of Agave americana
(Asparagaceae) and Echeveria sp. (Crassulaceae), Mar. 1913, A.
Tonelli.
Lit.: Ferraris (1914: 882), Prasil & de Hoog (1988: 53).
Original diagnosis (Ferraris 1914): Maculis subcircularibus luteolis;
caespitulis gossypino-pulverulentis, fulvis; hyphis sterilibus
intercellularibus, cylindraceis, septatis ramosis, 4–5 μm crass.;
conidiophoris caespituloso-erumpentibus epiphyllis, olivaceis,
lexuosis, septatis, simplicibus, 100–150 × 6–7 μm, subdenticulatoconidigeris; conidiis fulvo-olivaceis, continuis vel 1-septatis, 12–20
× 10–12 μm.
Notes: Prasil & de Hoog (1988) did not ind any authentic material
in FI, PDA, PAV, RO, ROPV and TO and considered it to be unlikely
that this forma was a true Cladosporium.
herbarum f. asparagi Thüm., Herb. Mycol. Oecon., Fasc. VII,
No. 320. 1875.
Type: USA, on Asparagus sp. (Asparagaceae), Thümen, Herb.
Mycol. Oecon. 320.
Notes: Status remains unclear.
herbarum f. carpophilum D. Sacc., Mycoth. Ital. 590. 1899.
Type: Italy, Selva, on dead fruit of Cheiranthus cheiri, Jul. 1899,
Saccardo, Mycoth. Ital. 590 (e.g., BPI 427188, SIENA).
Notes: A mixture of Cladosporium cladosporioides and C. herbarum.
herbarum f. imicola Marchal, Bull. Soc. Roy. Bot. Belgique
24: 67. 1885.
Type: Belgium, Brussels, on dung of shrew and mice, summer
1883 and winter 1883–1884 (in imo soricino et murino).
Lit.: Prasil & de Hoog (1988: 53).
Notes: No authentic material is maintained at BR. Probably this
was only a substrate form (Prasil & de Hoog 1988).
herbarum f. losculorum Thüm., Fungi Austr. Exs., Cent. XII,
No. 1173. 1874.
Type: Czech Republic, Bohemia, Teplitz, on Anthemis austriaca
(Asteraceae), summer 1873, von Thümen, Thümen, Fungi Austr.
Exs. 1173 (e.g., BPI 427196, HAL).
Notes: Often wrongly cited as C. herbarum var. losculorum Thüm.
No identiiable Cladosporium found in the material examined.
the genuS Cladosporium
herbarum f. fructicola Thüm., Fungi Austr. Exs. No. 266.
1872.
Type: Austria, Krems, on fruits of Datura stramonium (Solanaceae),
1871, A. Boller, Thümen, Fungi Austr. Exs. 266 (e.g., BPI 427198,
HAL).
Notes: Status remains unclear, a mixture of different hyphomycetes,
including Alternaria sp.
herbarum f. mesembrianthemi Rabenh., Herb. Mycol., Ed. II,
No. 767. 1858.
Syntype: Germany, Niedersachsen, Giesen, botanical garden,
on leaves of Mesembryanthemum umbellatum (Aizoaceae), H.
Hoffmann, Rabenhorst, Herb. Mycol. 767 (e.g., M).
Notes: True Cladosporium species, cladosporioides-like, but exact
identiication impossible without culture.
herbarum f. parasiticum Sacc., Ann. Mycol. 13(2): 133. 1915,
as “parasitica”. Fig. 396.
Holotype: On Taphrina tosquinetii (= Exoascus tosquinetii) on
leaves of Alnus glutinosa (Betulaceae), M. Weisskirchen (PAD).
Lit.: Saccardo (1931: 797), Prasil & de Hoog (1988: 54), Heuchert
et al. (2005: 53)
Ill.: Heuchert et al. (2005: 55, ig. 22).
Original diagnosis (Saccardo 1915b): Characteres morphologici a
typo vix diversi, sed stirps videtur biogena, parasitica.
In vivo: Colonies medium brown, effuse, conluent, caespitose.
Mycelium immersed and supericial; hyphae 3–5 μm wide,
septate, not or only rarely constricted at the septa, subhyaline to
pale brown, smooth, walls thickened, often somewhat swollen,
6–7 μm wide, swollen hyphal cells forming loose to dense
aggregations. Conidiophores solitary, arising from hyphae,
or forming loose fascicles arising from hyphal aggregations,
erumpent, erect to almost decumbent, straight to curved, more
or less geniculate-sinuous, unbranched, 52–82 × 3.5–5.5 μm,
slightly swollen at the very base, up to 7 μm, 0–3-septate, not
constricted at the septa, pale to medium brown, paler towards
the apex, walls somewhat thickened, occasionally with intercalary
swellings, up to 6 μm wide, swellings with conidiogenous loci,
but loci not conined to swellings. Conidiogenous cells integrated,
terminal and intercalary, 31–48 μm long, polyblastic, sympodially
proliferating, conidiogenous loci protuberant, thickened and
darkened-refractive, 1–2 μm wide. Conidia catenate, mostly
in branched chains, straight, subglobose, obovoid, ellipsoid,
subcylindrical, 5–20 × 3–5 μm, 0–1-septate, not constricted at
the septa, pale medium brown, smooth, walls unthickened to
slightly thickened, apex rounded to slightly attenuated, with up
to four hila, base truncate to convex, occasionally attenuated,
hila protuberant, thickened, darkened-refractive, 0.5–1.5 μm
wide, basal hilum up to 2 μm wide, microcyclic conidiogenesis
not observed.
Notes: Prasil & de Hoog (1988) examined the type material of this
forma and described it to be a mixed collection of C. herbarum
and C. cladosporioides. In the course of our own examinations, we
have not found any verruculose conidia which are characteristic
for C. herbarum. Furthermore, the conidia in this collection deviate
from C. herbarum in shape and width of the conidia (C. herbarum
conidia 4–7 μm wide). The conidiophores in f. parasiticum are also
characterised by being nodulose with conidiogenous cells as in C.
herbarum, but they are less consistent. Conidiophores and conidia
agreeing with those of C. cladosporioides have not been found in
the type collection.
www.studiesinmycology.org
Fig. 396. Cladosporium herbarum f. parasiticum (PAD). Conidiophores and conidia
in vivo. Scale bar = 10 µm. B. Heuchert del.
Cladosporium taphrinae, also occurring on Taphrina spp.,
inhabits the asci of Taphrina coerulescens, but the conidiophores
do not have any swellings and the apex is rugose-subdenticulate
by having numerous densely crowded conidiogenous loci. The
conidia are 0–2(–3)-septate. The conidiophores of C. phyllophilum
(= C. exoasci), which are also known from Taphrina spp., are often
decumbent to repent, but they are somewhat wider and intercalary
swellings are lacking. Furthermore, ramoconidia, 13–35(–38) ×
3–10 μm, mostly 1–5-septate, are formed.
This fungus is only known from the type specimen; additional
collections have not been seen. Therefore, a inal conclusion about
its taxonomic status is not yet possible.
herbarum f. Phaseoli vulgaris Thüm., Herb. Mycol. Oecon.
206. 1874, nom. nud. Fig. 397.
Type: Czech Republic, Böhmen, Tetschen, on leaves of Phaseolus
vulgaris (Fabaceae), Sep. 1873, von Thümen, Thümen, Herb.
Mycol. Oecon. 206 (M).
In vivo: Leaf spots amphigenous, scattered, at irst punctiform,
later enlarging, subglobose to somewhat irregular, 1–25 mm
diam, sometimes conluent, pale brown to somewhat reddish
brown, often spots somewhat raised, margin indeinite. Colonies
amphigenous, loosely caespitose, effuse, pale brown to medium
brown with dense fructiication. Mycelium internal, subcuticular to
349
BenSch et al.
Fig. 397. Cladosporium herbarum f. Phaseoli vulgaris (M). Conidiophores and conidia in vivo. Scale bar = 10 µm. K. Bensch del.
intraepidermal; hyphae branched, (2–)3–10 μm wide, septate, often
with small swellings and constrictions, sometimes swollen up to 15
μm, pale olivaceous or olivaceous-brown, smooth, walls slightly
thickened, somewhat aggregated or forming small stromatic hyphal
aggregations, subcuticular to intraepidermal, small, composed of
only few swollen hyphal cells, up to 30 μm diam, cells 5–10(–
14) μm diam, pale to medium brown, smooth, walls thickened.
Conidiophores solitary or in pairs, sometimes in small groups,
arising from hyphae, swollen hyphal cells or hyphal aggregations,
erumpent through the cuticle, erect, straight to slightly lexuous,
non-nodulose, not geniculate or only sometimes so, unbranched,
rarely once branched, 80–200 × 4–7(–8) μm, pluriseptate, usually
not constricted, medium brown, sometimes pales at the apex,
smooth, walls thickened, sometimes even two-layered, lumen
apparently somewhat aggregated at septa, somewhat wider at
the base, attenuated towards the apex; somewhat dimorph, few
small conidiophores intermixed, 15–60 × 3–4 μm, solitary or in
small groups, pale olivaceous or pale brown, smooth, walls almost
unthickened. Conidiogenous cells integrated, both terminal and
intercalary, proliferation sympodial, 14–40(–50) μm long, with
several conidiogenous loci per cell, loci somewhat protuberant,
truncate or slightly convex, 1–2(–3) μm diam, somewhat thickened
and refractive, sometimes darkened. Conidia in branched chains,
350
straight, subglobose, obovoid, ellipsoid to subcylindrical, 4–23(–
30) × (3–)4–6(–7) μm, 0–3(–5)-septate, mostly 0–1-septate, not
constricted at septa, pale olivaceous-brown or brown, smooth or
almost so, sometimes slightly rough-walled, walls unthickened or
only slightly thickened, between wall and lumen very pale, lumen
appears somewhat darker, slightly rounded or attenuated at apex
and base, hila more or less protuberant and truncate, (0.5–)1–2(–3)
μm diam, dome sometimes higher than the surrounding rim, up to
1 μm high.
Notes: Cladosporium leguminicola described from dead legumes
of Phaseolus differs in having shorter, 0–3-septate, fasciculate
conidiophores and verruculose conidia formed solitary or in
unbranched chains. Cladosporium pisicola on Pisum sativum and
C. cladosporioides differ in having somewhat narrower, thin-walled
conidiophores and 0–1(–3)-septate narrower conidia.
herbarum f. psoraleae Gonz. Frag., Intr. al. est. de la lor. de
micr. de Cat.: 152. 1917.
Type: Spain, on Psoralea sp. (Fabaceae).
Lit.: Gonzáles-Fragoso (1927: 198).
Original diagnosis (Gonzáles-Fragoso 1927): Manchas epiilas,
purpúreas; céspedes pequeños, escasos, sobre un pseudoestroma
inmergido; conidióforos fasciculados, rectos o lexuosos, sencillos,
the genuS Cladosporium
hasta de 40 × 5–5.5 μm, color ahumado claro; conidios ovoideooblongos, de 7–10 × 3–4 μm, continuos primero, luego 2-celulares,
casi hialinos o amarillentos.
Notes: Type not found. Status remains unclear.
herbarum f. repens Fresen., Beitr. Mykol. 1: 24. 1850.
Type: (Fresenius 1850: tab. 3, ig. 29: iconotype).
Notes: Fresenius (1850) described a “forma” repens without
indicating any morphological differences. A type collection was
not mentioned. Lindau (1907: 801) cited it as a synonym of C.
herbarum.
herbarum f. rhois Thüm., Fungi Austr. Exs., Cent. 6, No. 537.
1872.
Type: Austria, Lower Austria, Krems, on dead branches of Rhus
typhina (Anacardiaceae), winter 1870, A. Boller, Thümen, Fungi
Austr. Exs. 537 (BPI 427203, HAL).
Notes: Status unclear, immature, no identiiable Cladosporium
seen in the material examined. Sometimes wrongly cited as
C. herbarum var. rhois.
herbarum f. saxicola Sacc., Michelia 2(8): 578. 1882.
Type: USA, New Jersey, Newield, on wet stones, Ellis no. 3551
(PAD).
Lit.: Prasil & de Hoog (1988: 54).
Original diagnosis (Saccardo 1882): Conidia oblonga, 8–15 × 5,
plerumque 1-septata, olivacea.
Notes: Original specimen at PAD contains no fungus (Prasil & de
Hoog 1988).
herbarum var. aphidicola C. Massal., Madonna Verona 1918:
21. 1918.
Type: Italy, parasitic on aphids (PAD).
Lit.: Saccardo (1931: 798), Prasil & de Hoog (1988: 52).
Original diagnosis (Saccardo 1931): Hyphis fertilibus remote
septatis, cylindraceis, parum ramosis, 3–4 μm cr.; conidiis valde
polymorphis, modo ovalibus continuis, modo fusoideo-teretibus et
vulgo 2–3-septatis, utrinque interdum apiculatis, magnitudine valde
variis 6–20 × 3–6 μm.
Notes: Neither aphids nor Cladosporium conidia could be found in
authentic specimen at PAD (Prasil & de Hoog 1988).
herbarum var. aphidis Fuckel, Fungi Rhen. Exs., Fasc. 2, No.
111. 1863, nom. nud.
≡ Cladosporium herbarum var. aphidis Fuckel, Jahrb. Nassauischen
Vereins Naturk. 23–24: 356. “1869”, 1870, as “α aphidis”, nom. nud.
Lectotype (designated here): Germany, near Oestrich, on dead
carcass of aphids on Cornus sanguinea (Cornaceae), Fuckel,
Fungi Rhen. Exs. 111 (M-0057467). Isolectotypes: Fuckel, Fungi
Rhen. Exs. 111 (e.g. HAL).
Lit.: Saccardo (1886: 369), Lindau (1907: 830), Prasil & de Hoog
(1988: 52).
Notes: Prasil & de Hoog (1988) noted that type material is not
preserved in herb. G, but the material distributioned in “Fungi Rhen.
Exs. 111” is syntype material. This variety represents a mixture of
C. aphidis and C. herbarum.
herbarum var. brassicae Desm., in Thümen, Fungi Austr.
Exs. No. 1294. 1875.
Syntype: Czech Republic, Bohemia N., Decin (Tetschen), on
leaves of Brassica napus (Brassicaceae), spring 1873, de Thümen,
www.studiesinmycology.org
Fungi Austr. Exs. 1294 (e.g., BPI 427187, HAL).
Notes: Status unclear. No Cladosporium found in the material
examined.
\
herbarum var. cellulosae Sartory, R. Sartory, J. Mey. &
Baumli, Papier 38(1): 43. 1935? (not in this volume, probably
not in this journal).
Type: France, on rotten paper.
Lit.: Prasil & de Hoog (1988: 52).
Notes: Location of material unknown (no material in PC or STR);
possible identity with C. sphaerospermum.
herbarum var. cerealium Sacc., Ann. Mycol. 7: 285. 1909.
Type: Europe, on leaves, culms etc. of cereals (Triticum, Hordeum,
Secale etc.) (Poaceae) (PAD?).
Lit.: Lindau (1910: 795), Ferraris (1912: 333), Saccardo (1913a:
1366), Prasil & de Hoog (1988: 52).
Original diagnosis (Ferraris 1909): Stromatibus cellulosis, atrobrunneis; conidiophoris minutis, caespituloso-fasciculatis,
brunneis, septatis, plerumque simplicibus; conidiis catenulatis,
primum continuis deinde 1-, rar. 2-septatis, olivaceis.
Notes: The species was described as a substrate form on cereals
without any real morphological difference (Prasil & de Hoog 1988).
herbarum var. densum Roum., Fungi Sel. Gall. Exs., Cent. X,
No. 950. 1880.
Type: France, on leaves of Rhus cotinus (= Cotinus coggygria)
(Anacardiaceae), Roumeguère, Fungi Sel. Gall. Exs. 950.
Lit.: Oudemans (1921).
herbarum var. ephedrae Allesch., in herb. (M). Fig. 398.
Specimen: Germany, Berlin, botanical garden, on dead stems of Ephedra
gerardiana (= E. distachya) (Ephedraceae), Apr. 1894, P. Hennings, No. 38 (M).
In vivo: Colonies at irst punctiform, scattered, later conluent,
effuse, erumpent, densely caespitose, brown, velvety. Mycelium
internal, subcuticular or intraepidermal; hyphae branched, 4–10 μm
wide, septate, not constricted at septa, sometimes slightly so, pale
brown to pale medium brown, smooth, walls unthickened or almost
so, often aggregating forming stromatic hyphal aggregations.
Stromata well-developed, extended, 50–150 μm diam or even
larger, several layers deep, composed of swollen hyphal cells,
subglobose to somewhat angular, 8–13 μm diam, pale to medium
brown, smooth, walls somewhat thickened. Conidiophores in small
to usually moderately large or large groups, loose to somewhat
dense, arising from stromata, erumpent through the cuticle, erect,
straight to substraight, occasionally somewhat lexuous, nonnodulose, sometimes geniculate and once branched, 30–70(–85) ×
5–7(–8) μm, septate, not constricted at septa, pale to pale medium
brown, paler at apices, sometimes almost hyaline, smooth, walls
slightly thickened, often somewhat attenuated towards the apex.
Conidiogenous cells integrated, terminal or intercalary, 15–35 μm
long, proliferation sympodial, with a single or several, up to eight
conidiogenous loci per cell, loci protuberant, short cylindrical,
1.5–3 μm wide, up to 1 μm high, dome usually not higher than
surrounding rim, thickened and somewhat darkened-refractive.
Ramoconidia formed, subcylindrical-oblong, sometimes somewhat
irregular in outline, up to 27 μm long, usually 5 μm wide, pale
brown, smooth. Conidia catenate, in unbranched or branched
chains, straight or slightly curved, subglobose, obovoid, ellipsoid
to subcylindrical, 4–23 × 3–7.5 μm, 0–3-septate, not constricted
at septa, septa becoming somewhat sinuous with age, pale
351
BenSch et al.
Original diagnosis (Lagière 1945–1946): Conidiis majoribus: 27–
39(21–36) × 9–12 μm.
Notes: Original material seems to be lost. Prasil & de Hoog (1988)
consider it to be probably a Heterosporium species.
herbarum var. nigricans (Roth : Fr.) Fr., Syst. mycol. 3(2):
371. 1832, as “β nigricans”.
Type: On wood and other hard substrates.
Basionym: Byssus nigricans Roth, Catal. Bot. 1: 216. 1797.
= ? Dematium hippocastani Pers., Ann. Bot. (Usteri) 11: 32. 1794; Syn. meth.
fung. 2: 698. 1801.
Fig. 398. Cladosporium herbarum var. ephedrae (M). Conidiophores and conidia in
vivo. Scale bar = 10 µm. K. Bensch del.
brown, smooth or almost so, walls almost unthickened or slightly
thickened, attenuated towards apex and base, apex often broadly
rounded or attenuated, hila protuberant, truncate or slightly convex,
(0.5–)1–3 μm diam, thickenend, somewhat refractive; microcyclic
conidiogenesis occurring.
Notes: True Cladosporium species, but not identical with
C. herbarum. Status remains unclear.
herbarum var. fasciculare Corda, Icon. fung. 3: 9. 1839, as
“β fasciculare”.
Type: Czech Republic, on stems of umbellifers, Liliaceae and
Equisetum sp.
Lit.: Saccardo (1886: 351), Lindau (1907: 803), Ferraris (1912:
333), Prasil & de Hoog (1988: 52).
Ill.: Corda (1839: tab. 1, ig. 20).
Original diagnosis (Corda 1839): Caespitibus minutissimis,
gregariis, non conluentibus, atro-viridibus; stromate celluloso
immerso; loccis simplicissimis, fasciculatis, olivaceis; sporis
oblongis pallidioribus.
Notes: Type material is not preserved in PRM, but from the
igures it seems to be indistinguishable from C. herbarum (Prasil
& de Hoog 1988). Rabenhorst (1844) listed it as synonym of
C. fasciculare (Pers.) Fr. All samples from different herbaria
deposited as C. herbarum var. fasciculare proved to be C. herbarum
or C. macrocarpum. The collection in Allescher & Schnabl, Fungi
Bav. 592, belongs to C. macrocarpum.
herbarum var. lablab Sacc., Philipp. J. Sci. 18: 604. 1921.
Type: China, Foochow, on legumes of Dolichos lablab (Fabaceae)
(PAD).
Lit.: Saccardo (1931: 792), Prasil & de Hoog (1988: 52).
Notes: Authentic material at PAD contains C. herbarum,
C. cladosporioides, Alternaria alternata and Penicillium-like conidia
(Prasil & de Hoog 1988).
herbarum var. macrosporum Lagière, Ann. École Natl. Agric.
Grignon, Sér. 3, 5: 159. 1945–1946.
Type: France, Seine-et-Oise, Grignon, on leaves of Phleum
pratense (Poaceae), 1944, Lagière.
= ? Cladosporium phlei (C.T. Greg.) G.A. de Vries, Contr. Knowl. Genus
Cladosporium: 49. 1952.
Lit.: Prasil & de Hoog (1988: 53).
352
Lit.: Saccardo (1886: 351, 1931: 798), Lindau (1907: 803), Arens
(1945), Prasil & de Hoog (1988: 53).
Original diagnosis (Fries 1832): Caespitibus densis compactis
nigricantibus, ibris subobscuris.
Notes: Material could not be traced neither in herb. B nor PRM
(Prasil & de Hoog 1988).
herbarum var. phlei Lagière, Ann. École Natl. Agric. Grignon,
Sér. 3, 5: 159. 1945–1946.
Type: France, Seine-et-Oise, Grignon, on leaves of Phleum
pratense (Poaceae), summer 1944, R. Lagière.
Lit.: Prasil & de Hoog (1988: 53).
Original diagnosis (Lagière 1945–1946): Caespitulis erumpentibus,
foliicolis; hyphis noduloso-lexuosis, fuscis, 220–300 × 4–5 μm;
conidiis oblongis vel fusoideis, 1–3-septatis, tenuiter echinulatis,
18–24 (15–29) × 9–11 (7.5–12) μm.
Notes: Prasil & de Hoog (1988): “Original material is probably lost,
possible identity with C. macrocarpum.”
herbarum var. typharum Westend., Cryptogames: 26. 1854,
nom. inval.
Lit.: Oudemans (1919).
Notes: See entry below.
herbarum var. typharum Westend. & Van Haes., Cat. crypt.:
8. 1838. no. 173.
Notes: Cited in Lindau (1907: 801) as synonym of C. herbarum.
herbarum var. vitricola Sacc., Ann. Mycol. 7: 286. 1909.
Type: Italy, on dirty, wet glass (PAD).
Lit.: Lindau (1910: 795), Ferraris (1912: 334), Saccardo (1913a:
1366), Prasil & de Hoog (1988: 53).
Original diagnosis (Saccardo 1913a): A typo differt caespitulis
dendritico-radiantibus, sorididis.
Notes: “Byssocladium fenestrale auct. p.p. non Mart. nec Link”
(Saccardo 1913a: 1366). True Cladosporium species, but status
is not yet clear.
heuglinianum Thüm., Rev. Mycol. (Toulouse) 1: 11. 1879.
Type: Eritrea, near Nakfa on Red Sea (Nakfa ad Mare Rubrum), on
leaves of Buddleja polstachya (Scrophulariaceae).
Lit.: Saccardo (1892: 603).
Original diagnosis (Saccardo 1892): Maculis amphigenis, sparsis,
plus minus orbiculatis, magnitudine variis, nitido-atris, subeffusis,
velutinis; hyphis subrectis, subulato-rigidis, simplicibus, septatis,
6 mm. cr., longis, dilute fuscis; conidiis 1-septatis, cylindricosubellipticis, utrinque subacutis, non constrictis, 12–20 × 6–8 μm;
hyphis concoloribus.
Notes: Type material could not be located.
the genuS Cladosporium
hibisci Reichert, Bot. Jahrb. Syst. 56: 721. 1921.
Type: Egypt, near Siut, on dry stems of Abelmoschus esculentus
(≡ Hibiscus esculentus) (Malvaceae), Oct. 1822/25, Ehrenberg (B).
Lit.: Saccardo (1972: 1338).
Ill.: Reichert (1921: tab. 4, ig. 3).
Original diagnosis (Reichert 1921): Caespitulis effuses,
conluentibus, atris; hyphis fertilibus, non confertis, plerumque
solitariis, rectis, simplicibus, raro leniter nodosis, non lexuosis,
septatis, fuscis, 75–125 μm longis, 3–6 μm latis; conidiis
terminalibus elongatis, ellipsoideis, pallid-fuscescentibus vel
hyalinis non constrictis, 7–15 μm longis, 3–5 μm latis.
Notes: Zhang et al. (2003) cited collections on Hibiscus syriacus
(China, Shaanxi) and H. tiliaceus (China, Heilongjiang). It is now
treated at a synonym of C. sphaerospermum.
hydrangeae Z.Y. Zhang & T.F. Li, J. Anhui Agric. Univ. 26:
40. 1999.
Holotype: China, Liaoning, on living leaves of Hydrangea macrophylla
(Hydrangeaceae), 16 Sep. 1992, Wang & Li (MHYAU 07029).
Ill.: Zhang et al. (1999a: 41, ig. 1), Zhang et al. (2003: 110, ig. 70).
Original diagnosis (Zhang et al. 1999a): Maculis petalicolis vel
sepalis, distinctis vel conluentibus, atro-brunneis, 4 × 2 mm,
amphigenis, brunneis, atro-brunneis, longe undulatis purpuratis
vel fuscus brunneis, 10 × 2.5 cm. Myceliis immersis. Conidiophoris
solitariis, erectis, sinualatis, simplicibus, septatis, apice
sympodialiter elongatis, inlatis denticulatis, cicatricibus conspicuis,
brunneis, prope apicem pallescentibus, 25.2–121.0 (60.0) × 4.1–
5.1 (4.5) μm. Ramoconidiis longe cylindricis, 0–1 septatis, rarius
2–3 septatis, pallide brunneis, ad apicem inlatis denticulatis,
cicatricibus fuscis, 9.8–20.5 (14.3) × 3.9–5.1 (4.7) μm. Conidiis
catenatis, cylindricis, fusiformibus, 0–1 septatis, laevibus, pallide
brunneis, laxe vel utrimque cicatricibus protrudentibus praeditis,
6.2–22.6 (14.6) × 3.6–5.1 (4.8) μm.
Notes: Additional collections on Hydrangea macrophylla were
reported from Liaoning and Yunnan, China (Zhang et al. 2003), and
Lu et al. (2003) mentioned Viburnum macrocephalum f. keteleeri
as an additional host.
hypophloeum Berk. & M.A.Curtis, J. Linn. Soc., Bot. 10: 362.
1869.
Type: Cuba, on leaves of a Sapindaceae, February.
Lit.: Saccardo (1886: 359), Stevenson (1975).
Original diagnosis (Berkeley 1869): Maculis orbicularibus; loccis
lexuosis implexis; sporis curvulis oblongis triseptatis.
Specimen examined: Puerto Rico, limestone hills on the coast eight miles west of
Ponce, on leaves of Thouinia sp., 1 Dec. 1902, A.A. Heller, Porto Rican Fungi 6172
(B 700006554).
Notes: Type material of C. hypophloeum could not be traced and
is probably not preserved. A single collection from herb. B could
be examined and proved to be conspeciic with Pseudocercospora
thouiniae (F. Stevens) U. Braun & Crous.
insectorum Gonz. Frag., Broteria, Sér. Bot. 22: 69. 1926, as
“insectarum”.
Type: Spain, Madrid, botanical garden, on Saissetia hemisphaerica
(Hemiptera, Coccidae) on leaves of Asplenium lineatum
(Aspleniaceae) and Cordyline congesta (Asparagaceae), Mar.
1925, Menor.
Lit.: Saccardo (1972: 1338).
Original diagnosis (Gonzáles-Fragoso 1926): Caespitulis
aggregatis, effusis, ramosis, intricatissimis, pallide olivaceis, pauci
www.studiesinmycology.org
vel pluri-septatis, subtoruloideis, conidiophoris paucis distinctis,
conidiis terminalibus, subconcatenatis, concoloribus, oblongoovatis, 4–10 × 2.7–4 μm, continuis vel 1-septatis, rarissimis 5–4
locularibus.
Notes: Type collections could not be located in herb. MA.
iridicola Schwein., Trans. Amer. Philos. Soc., N.S., 4(2): 277.
1832.
Type: USA, Pennsylvania, Bethlehem, on leaves of Iris virginica
(Iridaceae), No. 2604.
Lit.: Saccardo (1886: 367, as “iridicolum”), Zhang et al. (1999b:
41−42).
Ill.: Zhang et al. (1999b: 41, Fig. 2).
Original diagnosis (Schweinitz 1832): C. minutissimum, sparsum,
acervulis nempe non conluentibus, quanquam e longrinquo
maculam sistunt latiusculam ob approximationem. Floccis
brevibus, cum sporidiis, fusco-nigris. Acervulis loccorum exsoletis,
quasi sphaeriaemorphibus.
Notes: Type material could not be located in herb. PH, it is probably
not preserved. A record of this species from China is reported by
Zhang et al. (1999b), but the name is probably misapplied.
javanicum Wakker, Meded. Proefstat. Oost-Java, N.S., 28:
1–9. 1896.
Type: Java, on cortex of (in corticibus) Saccharum oficinarum
(Poaceae).
Lit.: Saccardo (1899: 1082).
Ill.: Wakker (1896: tab. 1).
Original diagnosis (Saccardo 1899): Hyphis tenuibus, breve
ramosis; conidiis in ramorum apicibus oriundis, ovalibus, minutis,
2.5–3 × 1.5–2 μm.
juglandinum Cooke, Grevillea 16(79): 80. 1888.
Type: UK, Highgate, on fading leaves of walnut (Juglans regia,
Juglandaceae).
Lit.: Saccardo (1892: 604), Lindau (1907: 819).
Original diagnosis (Cooke 1888): Hypophyllous. Tufts small,
scattered, fuliginous. Threads septate, nodulose, rather lexuous,
dark brown below, paler above, mostly simple. Conidia acrogenous,
fusiform, uniseptate, translucent, 35 × 9 μm.
Notes: Type material of this species was not among a loan
of Cladosporium types form Kew (K). Based on the original
description (nodulose conidiophores and large conidia, 35 ×
9 μm), this species seems to be a synonym of Cladosporium
macrocarpum.
juncicola Rabenh. / P. Magnus, in herb., as juncicolum
Specimen: Egypt, on Juncus acutus? (Juncaceae), P. Ascherson, iter aegyptiacum
quartum, ex herb. P. Magnus (HBG).
Notes: On the label “viell. Brachysporium juncicolum Rabenh.;
Helminthosporium juncicolum Rabenh.”. Saccardo (1886: 430):
“Brachysporium juncicolum (Rabenh.) Sacc. Helminthosporium
juncicolum Rabenh., Bot. Zeit. 1851, p. 626 – An Cladosporium ?”.
kapildharense C.D. Sharma, Gadp., Firdousi, A.N. Rai &
K.M. Vyas, Indian Phytopathol. 51(2): 160. 1998.
Holotype: India, Madhya Pradesh, Shahdol circle, Amarkantak
(south forest division), Kapildhara, on living leaves of an unknown
hosts (“hospitis ignoti”), Jan. 1993, C.D. Sharma (S.U. Herb. No.
C.S.2). Isotype: IMI 356766.
Ill.: Sharma et al. (1998: 155, ig. 3).
353
BenSch et al.
Original diagnosis (Sharma et al. 1998): Maculae epigenosae,
expando per totam supericiem folii, brunneae atro. Coloniae
epiphyllosae, effusae, latescentes atro. Mycelium hypharum,
plerumque supericiales, angustum, septatum, laevia, et ramosum,
hyphae repentes, pallide vel moderate olivacea, usque 1–4.5
μm diametro. Stromata presentia, tenuiter evoluta, irregulares,
pseudoparenchymatosi, obscure, olivaceae. Conidiophori plerumque
solitaria raro caespitosi, macronematosi, mononematosi, usque
6 transversae septati, laevia, raro ramosi erecti vel suberecti, recti
vel lexuousi, cum tumoribus, pallide vel moderate olivacea, 9–116
× 1.5–5.5 μm. Cellulae conidiogenosae integratae, terminales
et intercalares, polyblastae, sympodiales, conidis orientibus ex
tumoribus, terminalibus, quae, iunt, postea intercalares, cicatricatae,
cicatricis distincto crassae, conidia simplicia, arida, catenata,
incatenis ramosis, acropleurogenosae, pallide olivacea, cylindrata,
doliiformia, ellipsiformia, fusiformia, ovata, globosa vel subglobosa,
apices obtusi, bases rotundatae vel obconico-truncatae, laevia,
usque 2 transverse septata, hilo distincto et crassae, interdum
eminentia, few conidis having banded septa, pallide olivacea, et
parvae, conidia hyalini, 1.5–26 × 1–6.5 μm.
Notes: Sharma et al. (1998) reported this species as being
morphologically similar to C. psoraleae and C. acaciicola but
different in its colour and nature of colonies, presence of poorly
developed stromata, size and colour of conidiophores and variable
conidia. However, type material could not be traced at herb. IMI
and is probably lost. Cladosporium acaciicola proved to be a black
yeast-like hyphomycete and, therefore, has been excluded from
Cladosporium s. str.
laricis var. pini-pineae Sacc. & Trotter, I Funghi dell’Avellinese,
Avellino: 154. 1920.
Type: Italy, Campania, near Avellino, mixed infection with
Pestalozzia hartigi, on sheaths of living leaves or leaves becoming
dry (“in vaginis adhuc vivis vel arescentibus foliorum”) of Pinus
pinea (Pinaceae).
Lit.: Saccardo (1931: 790).
Original diagnosis (Saccardo 1931): Caespitulis fusco-griseis,
subeffusis, in vaginis foliorum sitis; cetera eadem.
Notes: Type material of C. laricis could be re-examined and proved
to be synonymous with C. herbarum. The variety pini-pineae is
possibly an additional synonym.
lathyri Z.Y. Zhang & Y.L. Liu, J. Yunnan Agric. Univ. 15(3):
219–221. 2000.
Holotype: China, Liaoning, Shenyang, on leaves and stems of
Lathyrus quinquenervius (Fabaceae), 20 Oct. 1992, Y.X. Wang &
H. Li (MHYAU 07835).
Ill.: Zhang & Liu (2000: 219, ig. 1), Zhang et al. (2003: 115, ig. 74).
Original diagnosis (Zhang & Liu 2000): Maculis brunneis,
1–6 mm longis, coloniis dispersis, punctiformibus, aliquando
conluentibus, velutinis, olivaceis vel atro-brunneis. Myceliis
immersis. Stromatibus praeditis. Conidiophoris singularibus vel 3–4
fasciculatis, rectis vel geniculatis, septatis, simplicibus, ad apicem
1–3 cicatricibus conspicuis, nodis 5–8 μm crassis, pallide brunneis,
19.3–56.5 × 4.6–5.1 (av. 36.5 × 5.0) μm. Cellulis conidiogenis
conidiophoris coalitis, sympodialibus. Ramoconidiis longe
cylindricis, 0–1-septatis, ad apicem inlatis denticulatis, cicatricibus
ornatis, pallide brunneis, 10.3–21.0 × 4.1–5.1 (av. 14.3 × 4.2) μm.
Conidiis catenatis, fusiformibus, cylindricis, 0–1-septatis, laevibus,
cicatricibus protrudentibus praeditis, pallide brunneis, 8.9–20.6 ×
3.6–5.6 (av. 11.5 × 4.4) μm.
354
lauri Raybaud, Congr. Pathol. Végét. (Cent. Pasteur),
Strausbourg: 48. 1923.
Type: France, on leaves of Laurus nobilis (Lauraceae), on Aonidia
lauri (Hemiptera, Diaspididae) and Lecanium hesperidum.
Original diagnosis (Raybaud 1923): Lé mycélium de cette
moisissure est blanchâtre, il se développe surtout à la surface
inférieure des feuilles sous forme de taches couleur chocolat
formées par l’ensemble des conidiophores bruns et des conidies
moins brunes. Les taches se trouvent presque uniquement sur la
feuille qu’elles pénètrent au niveau des Cochenilles, qui sont ixées
à l’épiderme. ... Examinons d’abord le champignon développé
en dehors de la Cochenille, c’est-à-dire à la surface des feuilles.
Les conidies, nées le plus souvent le long des conidiophores ou à
leurs extrémités, sont le plus souvent unicellulaires ou bicellulaires.
Leurs dimensions varient de 3.5 μm à 5 μm de largeur et de 6 μm
à 8 μm de longueur. Mais il en existe de plus grandes qui peuvent
être tri- ou quadricellulaires. Parfois même nous en distinguons qui
se composent de cinq cellules; dans ce dernier cas, les cellules
centrales sont les plus volumineuses.
Notes: Type material could not be traced.
laxicapitulatum Matsush., Icones
Matsushima Lectorum: 35. 1975.
Microfungorum
a
Type: Japan, Hyogo, Kobe City, on a rotting leaf of Pasania edulis
(Fagaceae), Apr. 1969 (Matsush. herb. 2570).
Lit.: Ho et al. (1999: 131).
Ill.: Matsushima (1975: pl. 42, igs 1–2), Ho et al. (1999: 133, igs
24–25).
Original diagnosis (Matsushima 1975): Coloniae effusae, tenues,
fulvae. Mycelium immersum. Conidiophora solitaria, erecta, recta,
simplicia, septata, crassitunicata, (150–)200–300(–400) μm longa,
prope basin 6–8 μm crassa, sursum leviter angustata, prope apicem
4.5-5 μm lata, apice 2–4 dentibus conicis praedita, atrobrunnea
sursum brunnea. Conidia catenas ramosas ad dentes conidiophore
formantia, ellipsoidea, continua, pallide brunneae, 9–15 × 5–7.5
μm; ramoconidia continua vel raro 1-septatia, elliptico-cylindrica
vel ellipsoidea, brunnea vel pallide brunnea, usque ad 40 μm longa
et 8 μm crassa. Conidia ut in toto capitulum laxum deciduum fulvum
ad apicem conidiophore formantibus.
Notes: Type material not seen.
leprosum Morgan-Jones, Mycotaxon 6(1): 1. 1977.
Holotype: USA, Alabama, Lee Co., Auburn, on painted surface of
veneer, Apr. 1976, G. Morgan-Jones (BPI). Isotype: AUA.
Ill.: Morgan-Jones (1977: 2, ig. 1).
Original diagnosis (Morgan-Jones 1977): Coloniae effusae,
atrobrunneae vel fuscae, interdum olivaceo-brunneae, densae
vel sparsae, crustaceae vel pulveraceae, plerumque orbiculares.
Mycelium immersum vel supericial, ex hyphis ramosis, septatis,
pallide brunneis, 2.5–3.5 μm crassis compositum. Hyphae ex
cellulis inlates saepe compositae. Stromata frequenter adsunt
vel rudimentale, ex cellulis brunneis, farinacea, isodiametricis,
usque ad 10 μm crassa composita. Conidiophora macronemata vel
semimacronemata, mononemata, ex hyphis supericialibus oriunda,
simplicia vel ramosa, recta vel lexuosa, septata, plerumque inlates,
ad septa constricta, laevia vel farinacea, brunnea, apicem versus
pallidiora, 12–55 × 4–5 μm. Cellulae conidiogenae polyblasticae,
indeterminatae, in conidiophoris incorporatae, terminales, interdum
intercalares, sympodiales, cylindricae vel doliiformes, usque ad 5
cicatrices atro brunneae, applanatas, incrassates, protrudentes
gerentes. Conidia catenate, sicca, ellipsoidea vel doliiformia,
the genuS Cladosporium
pallide brunnea, laevia vel verruculosa, 0–1 septata, ad bases
cicatrice protrudenti et ad apice 1–4 cicatricibus protrudentibus,
9–16 × 4–5 μm.
Notes: Type material could not be traced in BPI and could therefore
not be re-examined.
linicola Pidopl. & Deniak, in Pidoplichko, Gribnaya Flora
Grubykh Kormov: 267. 1953, nom. inval.
Type: Ukraine, Kievsk. Oblast, on seeds of lax (Linaceae), also
found on damp hay.
Ill.: Pidoplichko (1953: 267, ig. 68).
Notes: Type material not traced, probably not preserved.
longipes Sorokin, On nekot. bolez. vinograda i drug. rast.
Kavkazk. Kraja, Tilis: 26. 1892. also in Z. Planzenkrankh.
3: 154. 1893.
Type: Caucasus, “im kubanischen Bezirk”, on leaves of Vitis
vinifera (Vitaceae).
Lit.: Saccardo (1895: 619).
Ill.: Sorokin (1893: tab. 4, igs 49–50).
Original diagnosis (Sorokin 1893): Nach ihrem äusseren Aussehen
sind die durch diesen Pilz verletzten Blätter den durch das
Cladosporium fasciculatum geschädigten sehr ähnlich. Hier inden
wir dieselben bräunlichen Flecken ohne deutliche Umrisse, mit
dunklen Punkten im Zentrum, welche den Wohnsitz des Parasiten
anzeigen. Aber bei der mikroskopischen Untersuchung ist ein
grosser Unterschied sogleich zu bemerken; hier haben wir ein
Bündel von Hyphen, welche auch aus einer Spaltöffnung der
Nährplanze herauskommen, aber sich durch eine ungewöhnliche
Länge unterscheiden. Keine der bekannten Cladosporium-Arten
hat solche langen fruchttragenden Hyphen; darum der Name Cl.
longipes. Das Bündel ist an der Stelle, wo es aus der Spaltöffnung
heraustritt, etwas angeschwollen, gleichsam eine Zwiebel bildend;
die hellgrauen Hyphen sind durch zahlreiche Querwände geteilt
und schnüren auf ihrer Spitze durchsichtige und farblose, längliche
Sporen ab. Die reifen Sporen sind durch 1–3 Querwände geteilt,
2–3 μm breit und 6–9 μm lang; sie entspringen nicht nur aus der
Spitze der Hyphe, sondern bisweilen auch an der Seite, so dass
man Präparate inden kann, wo die fruchttragende Hyphe nicht
eine sondern mehrere Sporen trägt. Innerhalb des kranken Blattes
indet man das Mycelium, welches aus Fäden von verschiedener
Dicke besteht. Im Protoplasma liegen hier und da glänzende
Öltropfen. Querwände hat Verf. im Mycelium nicht bemerkt.
Notes: Type material could not be traced. Status remains unclear.
lophodermii Georgescu & Tutunaru, Rev. Biol. (Bucharest)
3(1): 61. 1958.
Type: Romania, Poiana Stalin at Postăvaru, on apothecia of
Lophodermium pinastrum (Rhytismataceae) on needles of Pinus
sylvestris (Pinaceae), Jun. 1956.
Lit.: Heuchert et al. (2005: 56).
Ill.: Georgescu & Tutunaru (1958: 60, ig. 14).
Original diagnosis (Georgescu & Tutunaru 1958): Tapeta supra
discum, velutosa, bruneo, nigricantia et interdum fasciculis
hypharum bruneis ad basis et lateralia apotheciorum praedita.
Mycelium ante hypothecium e plectenchymo cellularum rotundarum,
ante hymenium ascarum hyphis solitarii, erectis irregulariter
curvatis, parce oblique sursum ramosis cum cellulis clavulatis.
Supra hymenium e his hyphis duo vel plures conidiophori erecti
irregulariter curvati atque geniculati lavobrunnei, hyalini, septis
sparsis, 110–130 μm longis et 4–5 μm crassis, cellula terminali
www.studiesinmycology.org
30–40 μm longa, apice ± clavuta evadunt. Conidia formiis variis,
irregulariter ellipsoidea et ovoidea brunnea vel ægre verrucata,
apice sæpe rotundata frequenter unicellularia 5–15 μm longa, 5–6
μm lata, bicellularia 10–18 × 5–8 μm, raro tricellularia 18–21 × 5.5
μm et quadricellularia 20–26 × 5.5–6 μm, ad septa non constricta.
Notes: Type material of this species was not available for a reexamination. The status of this species is unclear. According to
the original description and illustration, it is possibly a true member
of Cladosporium s. str. Järva & Parmasto (1980) recorded this
species from Estonia, but without any details.
lychnidis Z.Y. Zhang & Y.L. Liu, in Zhang, Liu, Wei & He, Plant
Diseases and Their Control: 104. 1998.
Holotype: China, Hubei, Wuchang, on Lychnis coronata
(Caryophyllaceae), 21 Sep. 1980, J.Y. Li & T.Y. Zhang, No. 13632
(MHYAU 03958).
Ill.: Zhang et al. (1998c: 105, ig. 2), Zhang et al. (2003: 118, ig.
76).
Original diagnosis (Zhang et al. 1998c): Coloniae effusae, tenuis
atro-brunneae, dispersae, punctatae vel patulae. Mycelium
immersum. Conidiophora solitaria, erecta, simplicia, septata, atrobrunneae, 33.4–56.5 × 3.3–3.3 μm, apice sympodialiter elongata
cicatricosa. Ramoconidia continua vel 1-septata, laevia, apice ±
inlata denticulata, basi atro-brunnea apicem versus brunnea,
7.7–13.1 × 4.6–7.2 μm. Conidia catenata, ellipsoidea, ovoides vel
subrotunda, continua vel raro uniseptata, laevia pallide brunnea,
4.1–12.3 × 2.8–3.1 μm.
Notes: Zhang et al. (2003) mentioned a second collection on
Dianthus chinensis.
lycopersici Plowr., Gard. Chron. 16: 621. 1881.
Type: UK, on fruits of Solanum lycopersicum (= Lycopersicon
esculentum) (Solanaceae).
Lit.: Saccardo (1892: 602), Ferraris (1912: 350).
Original diagnosis (Plowright l.c.): Hypha tufted, septate, irregular
in outline at their apices, springing by their bases from a black
spot; compacted mycelium, spores abundant, cylindrical, black,
1–3-septate, slightly pointed at their extremities; 10–30 mk. long,
by 8–10 mk. wide.
Notes: Status remains unclear. Type material could not be found
at Kew (K).
maculans (Catt.) Sacc. → oryzae Sacc. & P. Syd.
malvacearum C.D. Sharma, Gadp., Firdousi, A.N. Rai & K.M.
Vyas, Indian Phytopathol. 51(2): 156. 1998.
Holotype: India, Madhya Pradesh, Shahdol circle, Amarkantak
(south forest division), on living leaves of Kydia calycina
(Malvaceae), Jan. 1993, C.D. Sharma (S.U. Herb. No. C.S.5).
Isotype: (?) IMI 254691.
Ill.: Sharma et al. (1998: 154, ig. 2).
Original diagnosis (Sharma et al. 1998): Maculae amphigenosae,
fere irregulares et parvae sed avadentes magnae, et aetate
progrediente se extendents per totam supericiem folii atro. Coloniae
epiphyllosae, forma punctorum, tenuissimorum, glaucae atrae.
Mycelium hypharum, plerumque immersum, interdum supericiales
septatum, angustum, laeve et ramosum, hyphae repentes, medio
vel fusce brunneis, usque 2.5–5 μm diametro. Stromata presentia,
misere formata, pseudoparenchymatosa, obscure brunneae vel atro.
Conidiophori plerumque solitarii, interdum caespitosi, lax fasciculati,
macronematosi, mononematosi, usque 11 transversae septati
355
BenSch et al.
glabrae, raroramosi, erecti vel suberecti, parvum curvati, medio vel
fusce brunneae, 18.5–228 × 1.5–6.5 μm. Cellulae conidiogenosae
integratae, terminales et intercalares, polyblastae, sympodiales,
conidis, orientibus ex tumoribus, terminalibus, quae, iunt, postea
intercalares, cicatricatae, cicatrices, distinctae, crassae. Conidia
simplicia, catenata in catenis, ramosis, arida, acropleurogenosa,
cylindrata, doliiformia, ellipsiformia, fusiformia, ovata, globosa vel
subglobosa, apices obtusi, bases rotundatae vel obconico-truncatae,
laevia, usque 4 transverse septati, hilo distincto crassae interdum
non crassae, cum eminentia, plerumque moderate brunneae, et
parvae conidis fusce olivacea, 3–25.5 × 1.5–9.5 μm.
Notes: Data given on the label of the isotype collection in herb. IMI
(254691) [on Grewia sp. (Tiliaceae), India, Univ. Gorakhpur, A.N. Rai]
deviate from the data cited in the original diagnosis. Isotype material
of C. malvacearum has probably been mixed up with a second, quite
distinct collection. The collection on leaves of Grewia sp. showed
a Stenella-like or Ramichloridium-like hyphomycete. Sharma et
al. (1998) compared the symptomatological and morphological
features of C. malvacearum with those of earlier validly published
species of Cladosporium on various hosts stating that the species
differs considerably from all in its important taxonomical characters.
They reported C. malvacearum as showing some afinities with C.
apicale and C. uredinicola but being distinct in having amphigenous,
irregular, small to very large leaf spots and epiphyllous colonies, mid
to dark brown comparatively smaller conidiophores, and olivaceousbrown, up to 4 transversely septate, broad conidia.
manoutchehrii Esfand., Sydowia 5: 368. 1951.
Type: Iran, Polé Zangouleh, on living leaves of Quercus ×atropatena
(Fagaceae), 27 Jul. 1948, Manoutchehri.
Original diagnosis (Esfandiari 1951): Caespituli hypophylli,
raro etiam epiphylli, ambitu orbiculares vel omnino irregulares,
saepe conluentes et magnam folii partem aequaliter et
densissime obtegentes, obscure olivacei; conidiophora
solitaria vel saepe complura e hypostromate innato-erumpenti,
pseudoparenchymatico, pellucide olivaceo oriunda, divergentia,
recta vel plus minusve lexuosa, septata, 25–90 μm, raro usque
120 μm longa, 3–6 μm crassa, olivacea, simplicia vel apicem versus
2–3-furcata, ramulis inaequialtis, valde divergentibus; conidia
quoad formam et magnitudinem variabilissima, minora plerumque
plus minusve globosa, 2.5–4 μm diam., continua, majora ellipsoidea
vel oblonga, interdum subfusiformia vel clavato-oblonga, continua
vel circa medium septata, non vel lenissime constricta, mellea vel
pallide olivacea, 5–15 × 3.5–5 μm.
Notes: Esfandiari (1951) reported this species to occur in dense
caespituli mainly on the lower leaf surface on pale brown spots
caused by a gloeosporioid fungus, so that C. manoutchehrii is
probably only a secondary invader.
melanophlaei Thüm., Flora 60: 412. 1877.
Type: South Africa, Promont, near Grahamstown, on living
leaves of Rapanea melanophlaeos (≡ Myrsine melanophlaeos)
(Primulaceae), summer 1876, P. Mac. Owan, No. 1255.
Lit.: Saccardo (1886: 358).
Original diagnosis (Thümen 1877): Cl. in foliorum pagina
inferiore maculas indeterminatas, ibrosas, velutinas, explanatas,
inquinantes formans; hyphis longissimis, gracillimis, erectis,
subrectis vel minime curvatis, breviarticulatis, tenuissimis, fuscis;
sporis cylindraceis, apice rotundatis, basi obtusis, uniseptatis, 14–
16 mm. long, 3 mm. crass., pallide fusco-stramineis, subdiaphanis.
Notes: Type material could not be located.
356
metaplexis Z.Y. Zhang & X.Y. Wang, Mycosystema 19(2):
165. 2000.
Holotype: China, Heilongjiang, Harbin, Xiang-fang, on living leaves
of Metaplexis japonica (Apocynaceae), 2 Sep. 1992, Y.X. Wang &
H. Li (MHYAU 07830).
Ill.: Zhang et al. (2000a: 166, ig. 1), Zhang et al. (2003: 123, ig. 80).
Original diagnosis (Zhang et al. 2000a): Maculis angularibus,
irregularibus, pallide lavis vel lavido-brunneis, coloniis brunneis
vel atro-brunneis hypophyllis, dispersis vel interdum conluentibus,
1.5–5.0 × 1.5–4.0 mm. Myceliis immersis. Conidiophoris solitariis,
erectis, lexuosis vel undulatis, simplicibus, septatis, geniculatis,
pallide brunneis, versus apicem pallidis, 3–5-cicatricosis, 31–103
× 5.1–5.7 μm. Ramoconidiis continuis, rarenter ad apicem inlatis
denticulatis, basi cicatricibus conspicuis, 20.6 × 7.7 μm. Conidiis
solitariis, cylindricis, obclavatis, rectis vel sinuosis, 0–1-septatis,
raro 2–3-septatis, cellulis amplitudine inaequalibus, laevibus, pallide
brunneis, basi cicatricibus praeditis, 15.4–28.3 × 6.4–7.7 μm.
microporum Rabenh., Grevillea 17(83): 66. 1889.
Types: Italy, Sardinia, Gonnos-Fanadiga, on leaves of Nerium
oleander (Apocynaceae), Dr. Marcucci, Unio Itin. Crypt. 42 (e.g.,
B, HBG, M).
≡ Cladosporium microporum Rabenh., in Marcucci, Unio Itin. Crypt., No.
42. 1866, nom. nud.
Original diagnosis (Cooke 1889): Hypophyllum. Caespitulis
erumpentibus, gregariis, minutissimis, atris. Hyphis conidiisque – ?
Notes: Unio Itin. Crypt. (1866) is a selection of species made by
Marcucci and determinated by Rabenhorst. New species were
not validly published, because descriptions were not given. Cooke
(1889): “In our specimens only a minute species of Coniothyrium can
be found”. Saccardo (1892: 603, 1895: 617) changed the name to
C. microsporum Rabenh. Several duplicated of the original material
have been examined, but no trace of a Cladosporium was found.
microspermum Berk. & M.A. Curtis, Grevillea 3(27): 107.
1875.
Type: USA, South Carolina, on leaves of Quercus obtusiloba
(Fagaceae), No. 1686.
Lit.: Saccardo (1886: 360).
Original diagnosis (Berkeley 1875): Floccis tenuibus divaricatofurcatis articulatis; sporis minutis uniseptatis.
Notes: Zhang et al. (1998d) described and illustrated a collection
determinated as C. microspermum on Lithocarpus viridis from
China, which could not be checked.
microspilum Syd. & P. Syd., Ann. Mycol. 18: 102. 1920.
Type: Philippines, Luzon, Prov. Laguna, Mt. Maquiling, on leaves
of Cissampelos pareira (Menispermaceae), 26 Apr. 1919, T.
Collado, No. 6271.
Lit.: Saccardo (1931: 793).
Original diagnosis (Sydow & Sydow 1920): Maculae minutae,
orbiculares, 1–2 mm diam.; caespituli amphigeni, saepius
hypophylli, olivaceo-brunnei; hyphae fuscae vel fuscidulae, usque
400 μm longae, pluries septatae (articulis 20–50 μm longis), 4–5
μm crassae, rectae vel leniter curvatae; conidia sive continua 8–12
× 4–5 μm, sive 1-septata usque 20 μm longa, rarius 2-septata et
tunc usque 25 μm longa, non constricta, fuscidula, levia.
Notes: Type material could not be traced, neither in herb. B nor S.
microsporum Trab., in Roumeguère, Fungi Sel. Gall. Exs.,
Cent. XV, No. 1426. 1881.
the genuS Cladosporium
Type: Algeria, Trabut, on leaves of Nerium oleander (Apocynaceae).
≡ Bispora trabutiana Sacc., Bull. Soc. Roy. Bot. Belgique 31(2): 237.
1892.
Lit.: Saccardo (1895: 616), Lindau (1907: 768).
Original diagnosis (Saccardo 1895): Caespitulis hypophyllis,
exiguis, atris, e stromatum locellis oriundis; catenulis fasciculatis,
45–60 × 6–7 μm, hyphopodio brevi, 15 × 2.5 μm, continuio, simplici,
rarius furcate suffultis; conidiis ellipsoideis, utrinque obtusis, 8–9 ×
6–7 μm, ternissenis in quaque catenula, typice 1-septatis, non v.
vix constrictis, rarissime 3-septatis v. submuriformibus, fuligineis.
Notes: Type material was not available for a re-examination. Lindau
(1907) reported an additional collection of this species on leaves of
Rhododendron ponticum from the Netherlands.
miyakei Sacc. & Trotter, Syll. fung. 22: 1370. 1913.
≡ Cladosporium oryzae I. Miyake, J. Coll. Agric. Imp. Univ. Tokyo 2: 262.
1910, nom. illeg., homonym, non C. oryzae Sacc. & P. Syd., 1899.
Type: Japan, on leaves of Oryza sativa (Poaceae).
Lit.: Padwick (1950: 170).
Ill.: Miyake (l.c.: tab. 14, igs 68–70).
Original diagnosis (Saccardo 1913a): Mycelio supericiali,
repente, maculas nigras eficiente; conidiophoris prominentibus
obscuris, varie longis, plerumque 45–70 × 4–5 μm, septatis,
apice alterne denticulatis; conidiis brunneis, 1–4-sed plerumque
2-locularibus, magnitudine variabilibus, 7–20 × 4–6 μm, septis
constrictulis.
Notes: “A. Cl. maculani mycelio supericiali distinctum”. (Saccardo
1913a). Padwick (1950) cited a record of this species from Bengal,
India.
mori (Yendo) H. Zhang & Z.Y. Zhang, Proceedings of
Phytopathological Symposium Organized by Phytopathology
Laboratory of Yunnan Province 2: 306. 1998.
Basionym: Hormodendrum mori Yendo, Dai-Nihon Sanshi Kaiho
335: 6. 1919.
Type: Japan, on Morus sp. (Moraceae).
Ill.: Yendo (1919: ig. 2), Yendo (1927: 217–218, igs 91–92), Zhang
et al. (2003: 127, ig. 83).
Original diagnosis (Yendo 1919): Leaf spots distinct, irregular to
angular, vein-limited, later conluent and enlarged, greyish-brown.
Fruit bodies amphigenous, mainly hypophyllous dark indigo brown,
velvety. Hyphae immersed and creeping on the leaf surface.
Conidiophores emerging from creeping hyphae, straight, 235–290
× 5–7 μm, brown, simple or branched, 6–9-septate, occasionally
sympodially proliferating. Conidial scars thickened. Conidia in
simple or branched chain, lower conidia brown, 1–3-septate or nonseptate and 23–30 x 6–8 μm, pale brown, ellipsoidal to limoniform
and 6–10 × 5–6 μm.
Notes: Yendo (1919) provided a Japanese diagnosis for the new
species Hormodendrum mori, which has been kindly translated by
C. Nakashima (Japan). Zhang & Zhang (l.c.) assigned this species
to Cladosporium s. str. and mentioned several records from China
on Morus alba. However, neither type material of Hormodendrum
mori nor the Chinese collections have been available for a reexamination, so the taxonomic status of this taxon remains unclear.
A single collection on Morus from Germany proved to be conspeciic
with C. cladosporioides (Bensch et al. 2010).
multigeniculatum W. Yamam., Sci. Rep. Hyogo Univ. Agric.,
Ser. Agric. 4(1): 3. 1959, nom. inval.
Type: Japan, on Phyllostachys reticulata (Poaceae).
Lit.: Ho et al. (1999: 136).
www.studiesinmycology.org
Ill.: Yamamoto (1959: 4, igs 13–16), Ho et al. (1999: 135, igs
33–34).
Original diagnosis (Yamamoto 1959): Coloniis in agaro Czapeki
rapide crescentibus; caespitulis velutinis, pulverulentibus, planis,
interdum leniter sulcatis, olivaceis vel olivaceo-griseis, postea
fusco-olivacentibus, reversum viridi-atrum; mycelio compacto
intertexto, ex hyphis laxe ramosis, irregulariter septatis, ad septa
non vel parum constrictis, levibus vel scabriusculis, e subhyalino
pallide brunneis, 2.2–5.5 μm crassis composito; conidiophoris
in hyphis terminalibus vel lateralibus, simplicibus, raro ramosis,
rectis vel plus minus sinuosis, saepe dense irregulariterque
multigeniculatis, interdum 1–5 nodulosis, 6–27 vel pluriseptatis,
ad septa non vel parum constrictis, fuscis, sursum pallescentibus,
levibus, 160–880 × 4–5 μm, ramis 48–88 μm longis, raro usque
180 μm longis; conidiis acropleurogenis, catenulatis, in catenulas
dense ramosas dispositis; conidiis basis obclavatis vel obclavatocylindraceis, deorsum attenuato-truncatis, apice plus minusve
inlatis, saepe breviter pluraliterque proliferatis et irregulariter
geniculatis, apice cum pluraliter hilis, continuis vel 1–2-septatis,
pallide olivaceis, levibus, 13–34 × 3–5 μm; conidiis supernis
ellipsoideis vel ovoideis, raro subglobosis vel oblongis, raro
subglobosis vel oblongis, interdum apice cum 1–5 hilis, continuis
vel 1-septatis, levibus vel minutissime verruculosis, e subhyalino
pallide olivaceis, 4–12 × 2.5–4.5 μm.
Notes: Yamamoto did not designate a type. Ho et al. (1999)
selected igures 33/34 as “lectotype” (iconotype) of this species,
with lyophilized culture of ATCC 38012, batch 12-13-78 as epitype.
This “lectotypiication” is, however, incorrect since the igures
cited are not elements from the protologue. Hence, the name
C. multigeniculatum was not validated by Ho et al. (1999). A dried
culture of ATCC 38012 has been examined (BPI 746095). This
material does not fully agree with the original description [the long
iliform conidiophores are not or only slightly geniculate in the upper
part, i.e., not strongly and densely geniculate as depicted in the
original drawing, and the conidia are aseptate, (2–)4–18 × (2–)3–
4(–5) μm (septate, much larger ramoconidia as originally described
and illustrated have not been found]. The material examined agrees
well with C. tenuissimum, except for the somewhat geniculate
conidiogenous cells. Hence, the status of C. multigeniculatum
remains unclear. The CBS culture CBS 122130 which probably
represents an authentic strain of C. multigeniculatum was included
in the phylogenetic analyses of the C. cladosporioides complex
and proved to be conspeciic with C. cladosporioides (Bensch et
al. 2010).
myrmecophilum (Fresen.) Bayl. Ell., Trans. Brit. Mycol. Soc.
5(1): 138. 1914.
Type: Germany, in a nest of Lasius fuliginosus (insect).
Basionym: Septosporium myrmecophilum Fresen., Beitr. Mykol. 3:
49. 1863.
≡ Macrosporium myrmecophilum (Fresen.) Sacc., Syll. fung. 4: 538.
1886.
≡ Cladotrichum myrmecophilum (Fresen.) Lagerh., Entomol. Tidskr.
1900: 17. 1900.
Lit.: Saccardo (1931: 798).
Ill.: Fresenius (1863: tab. 6, igs 29–32), Bayliss Elliott (1914: tab.
2, igs 1–4).
Original diagnosis (Fresenius 1863): Schwarze, oft bündelweise
zusammenstehende, einfache oder auch mitunter ästige, gerade
oder mehr oder weniger gekrümmte Fäden, aus einer kriechenden,
torulos-zelligen Basis. Sie sind unten dichter, oben entfernter
querwändig und verdünnen sich nach oben allmählich. Sporen in
357
BenSch et al.
verschiedener Grösse und Entwicklung, einfach, zweizellig und
mehrzellig. Die letzteren ausgebildeten haben eine rundlich-eckige
Form und messen 1/80–1/50 mm; sie sind undurchsichtig und
nur mit Mühe sieht man eine Spur ihrer Zellentheilung, während
die jüngeren durchsichtig sind. Auch die Fäden sind durchsichtig.
Ansitzende Sporen waren nirgends zu inden; sie lagen in ihren
verschiedenen Entwicklungsformen zwischen der Basis der Fäden
und die Spitze der letzteren erschien immer scharf abgestutzt.
Notes: Type material is not preseved. Status remains unclear.
Material examined by Jessie S. Bayliss Elliott was supplied by
H. Donisthorpe from freshly excavated ant (L. fuliginosus and
L. umbratus) nests. Further designation or deposit of material
was not speciied by Elliott. Fresenius (1863) described his new
species from the surface of a nest of Formica fuliginosa (= Lasius
fuliginosus) consisting of wood ibres of Picea.
neocheiropteridis Y.L. Liu & Z.Y. Zhang, Mycosystema 19(2):
169. 2000.
Holotype: China, Yunnan, Kunming, Jindian, on living leaves of
Neocheiropteris palmatopedata (Polypodiaceae), 4 Apr. 1997, K. Li
& H. Xiong (MHYAU 07827).
Ill.: Liu et al. (2000: 170, ig. 1), Zhang et al. (2003: 129, ig. 85).
Original diagnosis (Liu et al. 2000): Maculis amphigenis,
irregularibus, fulvis, 2–11 mm diam., postea interdum perforatis.
Coloniis in PDA effusis, velutinis, fulvis. Conidiophoris fasciculatis,
rectis vel lexis, non ramosis, septatis, pallide brunneis, 27.6–141.0
× 3.8–8.0 μm. Cellulis conidiogenis in conidiophoris coalitis,
sympodialibus. Cicatricibus conspicuis. Ramoconidiis 0–1 septatis,
laevis, 10.1–30.1 × 7–7.7 μm. Conidiis globosis, ovatis, ellipsoideis,
pallide brunneis, laevis, 0–1 septatis, 3.7–8.8 × 3.7–7.8 μm.
neottopteridis Y.L. Liu & Y.H. He, Mycosystema 19(2): 169.
2000.
Holotype: China, Yunnan, Kunming, Jindian, on living leaves of
Asplenium nidus (≡ Neottopteris nidus) (Aspleniaceae), 4 Apr.
1997, K. Li & H. Xiong (MHYAU 07828).
Ill.: Liu et al. (2000: 170, ig. 2), Zhang et al. (2003: 130, ig. 86).
Original diagnosis (Liu et al. 2000): Maculis amphigenis, saepe
margine folii occupantibus, ellipsoideis, atro-brunneis, 3–8
mm diam., postea interdum perforatis. Coloniis in PDA effusis,
velutinis, atro-brunneis. Conidiophoris fasciculatis, rectis, non
ramosis, septatis, atro-brunneis, 190–563 × 3.5–7.7 μm. Cellulis
conidiogenis in conidiophoris coalitis, sympodialibus. Cicatricibus
conspicuis. Ramoconidiis 0–2 septatis, brunneis, laevis, 6.1–23.1
× 3.8–6.2 μm. Conidiis fusiformibus, ovatis vel ellipsoideis, 0–1
septatis, brunneis, laevis, 2.7–12.6 × 2.8–6.4 μm.
nervisequum Mont., Ann. Sci. Nat. Bot., Sér. 4, 8: 298. 1857.
Type: France, on leaves of Eriobotrya japonica (Rosaceae), L.
Castagne, No. 2789.
Original diagnosis (Montagne 1857): Maculae variae magnitudinis
in pagina foliorum superiori conspiciuntur ex epidermide emortua
dealbataque griseae, subtus ruidulae, initio orbiculares tandem
conluentia, ut evenire solet, irregulares, omnes limbo fusco plus
minus lato ut in Depazeis vel Phyllostictis cinctae. Fibrarum fasciculi
e issuris cuticulae erumpentes nervos ultimi ordinis folii sequentes
et tum reticulum visu mirabile efformantes. Fibrae ipsae stromate
celluloso junctae, basi ob cellulam globosam subbulbosae, tunc
erectae, lexuosae, tunc apice declinatae, ramum plerumque
singulum gerentes, inaequales et inaequaliter septatae, septis
modo appressis modo laxiusculis, oculo nudo vel armato atrae, at
358
microscopii ope visae rufo-fuscae, 1/5–1/8 millim. longae, 0mm,0050
ad 0mm,006 crassae, sensim a basi ad apicem saepe decolorem
et fructiicantem attenuatae. Sporae acrogenae, initio continuae,
ovoideae, mox deciduae, oblongae, septo transversali divisae,
tandem septis ternis quadriloculares, longitudine inter 0mm,005 et
0mm,01 variabiles.
nicotianae Oudem., Ned. Kruidk. Arch., Ser. 3, 2(3): 769.
1902.
Type: Netherlands, Amerongen, on decaying leaves of Nicotiana
tabacum (Solanaceae), Jul. 1901, C.J. Koning (L).
= Cladosporium tabaci Oudem., Beih. Bot. Centralbl. 11: 538. 1902.
Lit.: Saccardo (1906: 576), Lindau (1907: 829), Oudemans (1923),
de Vries (1952: 94).
Original diagnosis (Oudemans 1902b): Hyphes en touffes
médiocrement denses, en partie diffuses, en partie dressées,
simples, lexueuses, à peine noueuses, couleur noisette, 150–170
× 20 μm, terminées par une conidie elliptique, 8 × 4–5 μm, d’abord
hyaline, plus tard couleur noisette, ordinairement 1-septée.
Notes: The type is represented by only two drawings, the
description is short and the drawings are so poor that this species
was regarded as doubtful [a nomen dubium] (de Vries 1952).
nitrariae Dumitraş & Bontea, Rev. Roumaine Biol., Sér. Bot.
12(6): 387. 1967.
Type: Romania, Ploeşti, between Pîclele Mari and Pîclele Mici, on
living leaves, fruits and twigs of Nitraria schoberi (Nitrariaceae), Jul.
1962, Tiberiu Oprescu (BUCM).
Ill.: Bontea & Dumitraş (1967: 388, ig. 2).
Original diagnosis (Bontea & Dumitraş 1967): Conidiophoris brunneis
apice pallidioribus, erectis vel geniculatis, 3–5 septatis, rare continuis,
17–57 × 3–5 μm, solitariis vel 5–8 fasciculatis. Conidiis terminalis,
ovatis, elongatis vel globosis, uni- rare 2-septatis, primum hyalinis
deinde pallide brunneis vel atro-brunneis, 4–12 × 2–5 μm, solitaribus
vel breviter catenulatis, facile caduci.
Notes: Cladosporium nitrariae parasitizes all aerial parts of its
host plant Nitraria schoberi, an endemic plant in Romania, but
particularly the fruits and apices of young shoots. Discoloured
zones bordered by a blackish brown margin appear on fruits, which
soon blacken due to the formation of the fructiication of the fungus.
The early attacked fruits wrinkle, dry and fall before maturation.
The apices of attacked shoots defoliate prematurely and present
blackish, elongated spots, sometimes comprising the shoot all
around (Bontea & Dumitraş 1967). Type material of this species
was not available for a re-examination.
olivaceum (Corda) Bonord., Handb. Mykol.: 72. 1851.
Type: Czech Republic, on rotten wood of Picea (Pinaceae).
Basionym: Mydonosporium olivaceum Corda, Deutschl. Fl. 3(13):
95. 1833.
Lit.: Saccardo (1886: 354), Lindau (1907: 811).
Ill.: Corda (1833: tab. 48).
Original diagnosis (Corda 1833): Acervuli minuti olivacei; loccis
olivaceis septatis, subimpellucidis; sporis didymis luteis diophanis;
muco colorato.
ophiopogonis T. Zhang & Z.Y. Zhang, Plant Diseases and
Their Control: 110. 1998.
Holotype: China, Zhejiang, Hangshou, on living leaves of
Ophiopogon mairei (Asparagaceae, Liliaceae s. lat.), 3 Nov. 1980,
J.Y. Li & T.Y. Zhang, No. 44132 (MHYAU 03951).
the genuS Cladosporium
Ill.: Zhang & Zhang (1998b: 110, ig. 2), Zhang et al. (2003: 136, ig.
91; pl. 13, ig. 2).
Original diagnosis (Zhang & Zhang 1998b): Maculata amphigena,
subrotunda vellonge-ellipsoidea, ochracea, lineolata red-brunnea
margina, prominula, 1–2(–5) mm longa. Conidiophora solitaria,
erecta, simplicia, septata, recta vel sinuolata, sympodialiter prolifera
denticulata, lavo-brunnea sursum pallescentia, 54.0–244.0 ×
4.6–5.9 μm. Ramoconidia continua vel raro 1–2 septata, denticulata, pallide brunnea, 5.1–15.4 × 3.1–5.1 μm. Conidia catenata,
continua, fusiformia, longe-ellip-soidea, sphaerica, laevia, pallide
brunnea, hili non evident, 2.8–11.6 × 2.8–4.6 μm.
Notes: Chlorophytum elatum was given as an additional host (Zhang
& Zhang 1998b). Since hila have been described as “non evident”
this species probably does not belong in Cladosporium s. str.
oplismeni Syd., Philipp. J. Sci., (Ser. C., Bot.) 8: 507. 1913.
Type: Philippines, Luzon, Ifugao, Mt. Polis, on spikes of
Oplismenus hirtellus ssp. undulatifolius (≡ O. undulatifolius)
(Poaceae), McGregor.
Lit.: Saccardo (1931: 792).
Original diagnosis (Saccardo 1931): Caespitulis crassis, fructus
totos densissime obte-gentibus et eos omnino destruentibus,
olivaceo-brunneis; hyphis simplicibus, remote septatis, olivaceobrunneis, usque ad 90 μm longis, 3–5 μm crassis; conidiis olivaceobrunneis, continuis, 6–10 × 3.5–4.5 μm, vel elongatis, 1–3-septatis
et tunc usque ad 20 μm longis, levibus.
Notes: Type material could not be traced, neither at herb. B nor at
herb. S.
oryzae I. Miyake → miyakei.
oryzae Sacc. & P. Syd., Syll. fung. 14: 1082. 1899.
Type: Italy, on rotten culms of Oryza sativa (Poaceae).
≡ Helminthosporium maculans Catt., Arch. Triennale Lab. Bot. Crittog.
2/3: 122. 1879.
≡ Cladosporium maculans (Catt.) Sacc., Syll. fung. 4: 365. 1886, nom.
illeg., homonym, non C. maculans Schwein., 1832.
Lit.: Lindau (1907: 814), Ferraris (1912: 339), Padwick (1950: 170).
Ill.: Cattaneo (1879: tab. 14, igs 7–9).
Original diagnosis (Saccardo 1886): Caespitulis discoideis, carnosoibrosis; hyphis simplicibus, fasciculatis, erectis, septatis, luteofuscis; conidiis minutis, oblongis, ditridymis, hyalinis, 15 × 6 μm.
Notes: Type material could not be traced. No authentic material in
Saccardo’s herbarium (PAD). All collections examined, deposited
under C. oryzae did not contain any Cladosporium species.
Oudemans (1923) cited Jasminum fruticans as host species, but
this record presumably refers to C. maculans Schwein.
ovorum Pidopl. & Deniak, in Pidoplichko, Gribnaya Flora
Grubykh Kormov: 272. 1953, nom. inval.
Type: Ukraine, on a hen’s egg.
Ill.: Pidoplichko (1953: 272, ig. 73).
Notes: Type material probably not preserved.
pallidum Berk. & M.A. Curtis, Proc. Amer. Ackad. Arts 4: 127.
1858, 1860.
Type: Nicaragua, Greytown, on leaves of an unidentiied host,
1856, U.S. Pac. Ex. 354 (K).
≡ Cercospora pallida (Berk. & M.A. Curtis) Cooke, Grevillea 17(81): 21.
1888, nom. illeg., non C. pallida Ellis & Everh., 1887.
Lit.: Saccardo (1886: 361, 1892: 638), Chupp (1954: 609), Crous &
Braun (2003: 304).
www.studiesinmycology.org
Original diagnosis (Berkeley & Curtis 1860): Maculis
orbicularibus pulveraceis; floccis erectis simplicibus; sporis
oblongis sinuatis.
pallidum (Oudem.) H. Zhang & Z.Y. Zhang, Proceedings of
Phytopathological Symposium Organized by Phytopathology
Laboratory of Yunnan Province 2: 306. 1998, nom. illeg.,
homonym, non C. pallidum Berk. & M.A. Curtis, 1860.
Basionym: Hormodendrum pallidum Oudem., Arch. Néerl. Sci.
Exact. Nat. 7: 293. 1902.
Type: Netherlands, Bussum, from soil.
Lit.: Saccardo (1906: 581), Zhang et al. (2003: 233).
Ill.: Zhang et al. (2003: 233, ig. 152).
Notes: Zhang et al. (2003) recorded this species from China on
Antirrhinum majus and Enkianthus quinquelorus. In any case,
the description and illustration of “C. pallidum” from China do not
agree with the original description of Hormodendrum pallidum by
Oudemans, who described branched (dendroid) conidiophores and
much wider conidia, 12–20 × 5–8 μm.
penicilloides Preuss, Deutschl. Fl. 3(26): 31. 1848.
Holotype: Germany, on Tubercularia granulata and T. vulgaris
(anamorphic fungi), C.G.T. Preuss, Nr. 396 (B 700006672).
Lit.:Heuchert et al. (2005: 56).
Ill.: Preuss (1848: pl. 16).
Original diagnosis (Preuss 1848): Caespitus effusis, olivaceis,
crassis; loccis erectis, longis, irregulariter ramosis, ramulisque
intricatis, fuscis, septatis, polymorphis; sporis ovatis, obovatis,
oblongis, subrotundis, bisulcisve multiformibus; episporio hyalino,
hylo basilari saepe instructo; nucleo irmiusculo diaphano.
Specimen examined: Italy, Veneto, Prov. di Treviso, Selva di Cadore, on
Prunus domestica, Sep. 1875, P.A. Saccardo, Saccardo, Mycoth. Ven. 587 (B
700006671).
Notes: Type material and an additional collection from Italy have
been examined, but proved to be in very poor condition, not allowing
a inal conclusion about the taxonomic status of this species.
Nannizzi (1934) considered C. madagascarense a synonym of
C. penicilloides. Cladosporium madagascarense, described as
isolated from a human, is, however, a doubtful, excluded name
(Dugan et al. 2004). Saccardo (1886) cited C. penicilloides as found
on chrysalises at still attached leaves of Prunus domestica in north
Italy. Oudemans (1920) listed Betula verrucosa and (1921) Prunus
domestica as hosts. Bontea (1985, 1986) reported it from Romania
on Calycanthus loridus, C. laevigatus and C. occidentalis.
perpusillum Sacc., Atti Reale Ist. Veneto Sci. Lett. Arti, Ser. 6,
2(3): 449. 1883–1884.
Type: France, Vendée, Malbranche, on culms of Ammophila sp.
(Poaceae), no. 78.
Lit.: Saccardo (1886: 364).
Original diagnosis (Saccardo 1883–1884): Caespitulis creberrimis,
punctiformibus, atris, supericialibus; hyphis e basi stromatica
hemisphaerica sphaeriaeformi oriundis, iliformibus, 40–50 × 5
μm, sursum denticulato-sporigeris, continuis, fuligineis; conidiis
oblongis v. breve fusoideis, continuis mox 1- (raro 2–3)-septatis,
vix constrictis, 9–11 × 4 μm, olivaceis.
Notes: Type material could not be found. Status remains unclear.
peruamazonicum Matsush., Matsushima Mycol. Mem. 7: 47.
1993.
Type: Peru, Rio Ampiyacu, on rotten leaves, Oct. 1992.
359
BenSch et al.
Original diagnosis (Matsushima 1993): OBS in b/c – Coloniae
effusae, luteo-brunneae. Conidiophora mononematica, erecta,
recta, cylindrical, simplicia, septata, rigida, laevia, 50–100 μm longa,
3.0–4.0 μm crassa, circa basim atro-fusca, supra fusca, cellula
terminali pallidiora sporadice verrucata, p.m. facile disarticulata,
quae apice polyblastica denticulataque, conidiorum catenibus
simlicibus acropetalibus blastogenis 3–7-seriebus dispositis ferens.
Ramoconidia ubi presentia, 18–31.5 μm longa, 0–1-septata, apice
polyblastica, denticulata, sporadice verrucata, pallide vel modice
fusca. Conidia 1-septata, rarissime 2-septata, praecique sporadice
verrucata, sicca, pallidissime brunnea, modice luteo-brunnea in
massa; conidia primo (et interdum secundarie) formata clavata,
18.5–36.5 × 3.5–5.0 μm, conidia postea formata ellipsoidea 12–22
× 4.5–6.5 μm. In CMA – Coloniae p.m. lente crescentes, pallide
luteo-brunnea, reverso atro-fusco.
Notes: Type material was not available.
phlei-pratensis Sawada, Bull. Gov. Forest Exp. Sta. 105: 96.
1958, as “phlei-pratense”, nom. inval.
Types: Japan, Tohoku District, on leaves of Phleum pratense
(Poaceae), 27 Jul. 1940, Iizuka (10516) and 18 Jul. 1941, Ikeda
(10440).
Original diagnosis (Sawada 1958): Macula phyllogena, sparsa,
fusiformis vel utrimque truncata, fulva vel centro rotunde fulva vel
cana et circum fulva, circa 3 mm longa. Nonnulla–10 et nonnulla
conidiophora densa badia, cylindrica, continua, 70–131 × 4.5–6 μm,
apice cum nonnullis conidiis. Conidium ellipticum vel oblongum,
0–3 septatum, apud septa non constrictum vel constrictum, fuscum,
6–20 × 3–6 μm.
Notes: Type material could not be traced. In the original diagnosis
two collections were mentioned, but the author did not designate
a type. Cladosporium phlei, known from Asia, Europe and North
America on Phleum pratense, deviates from the description of
Cladosporium phlei-pratensis given above in having longer and
wider conidiophores, up to 300 × 6–9 μm, and longer and wider
conidia, 13–36(–57) × 6–14 μm (David 1997).
pilicola Richon, Cat. Champ. Marne: 452. 1889.
Type: France, Marne, Soulanges, on dry stems of Galium mollugo
(Rubiaceae).
Lit.: Saccardo (1892: 602, as “pilicolum”).
Original diagnosis (Saccardo 1892): Hyphis fasciculatis, brunneis,
ramosis; conidiis cylindraceis, 1–3-septatis, concoloribus.
Notes: Type material undoubtedly not preserved. Status remains
unclear.
pisi Cugini & Macch., Boll. Reale Staz. Agric. Modena, N.S.,
10(1890): 104. 1891.
Type: Italy, Vaciglio near Modena, on legumes of Pisum sativum
(Fabaceae).
Lit.: Saccardo (1892: 601), Lindau (1907: 825), Ferraris (1912:
349), Oudemans (1921: on Vicia faba), Gonzáles-Fragoso (1927:
203, on Phaseolus and Pisum), Snyder (1934: 890), Morgan-Jones
& McKemy (1992: 11).
Ill.: Cugini & Macchiati (1891: tab. 5).
Original diagnosis (Saccardo 1892): Caespitulis fuscis, minutissimis
in pustulis ex epicarpio deformato exortis insidentibus; hyphis
elongatis, ramosis, lexuosis, crebre septatis, brunneis; hyphis
fertilibus, pallide fuligineis; conidiis terminalibus, ovatis, subhyalinis,
saepius 1-septatis, constrictis, 2-guttatis, 4.5–5.5 × 4–4.5 μm,
lateralibus ellipsoideis, continuis, hyalinis, 7–9 × 2.5–4.5 μm.
360
Notes: The status of this taxon is uncertain (Farr et al. 1989). Type
material could not be traced. Material from Spain, recorded by
Gonzáles-Fragoso (1927), has been re-examined and proved to be a
separate new species, viz., C. leguminicola. Gonzáles-Fragoso (1927)
did not provide any new morphological details, but just translated the
original description into Spanish. The original description of very
small, subhyaline conidia, 4.5–5.5 × 4–4.5 μm (terminal) and 7–9 ×
2.5–4.5 μm suggest a synonymy with C. cladosporioides, but without
type material, a inal conclusion is impossible.
platycodonis Z.Y. Zhang & H. Zhang, Mycosystema 19(3):
308. 2000.
Holotype: China, Heilongjiang, Monte Maoer, on living leaves and
petals of Platycodon grandilorus (Campanulaceae), 5 Sep. 1992,
H. Li & Y.X. Wang (MHYAU 07826).
Ill.: Zhang et al. (2000b: 309, ig. 1), Zhang et al. (2003: 145, ig.
97).
Original diagnosis (Zhang et al. 2000b): Maculae in petalis evolutae
vel amphigenae, circulares, brunneae, margine atrobrunneae, 1–2
mm diam. Coloniae punctiformes, interdum conluentes, velutinae,
fuscae. Mycelium immersum. Conidiophora solitaria vel fasciculata,
erecta vel leniter curvata, simplicia, septata, geniculata, denticulata
vel cicatricosa, brunnea, sursum pallescentia, 29.6–110.5 × 3.6–
4.6 μm. Ramoconidia continua vel raro 1-septata, ellipsoidea,
ellipsoideo-cylindrica, apice denticulata vel cicatricosa, pallide
brunnea, 7.2–19.3 × 2.6–4.7 μm. Conidia catenata, continua vel
1-septata, fusiformia, longe ellipsoidea, laevia, pallide brunnea vel
hyalina, 5.1–16.7 × 3.1–5.1 μm, sporarum cicatrices visibiles.
Notes: Zhang et al. (2000b) reported the species also on
Adenophora stricta from Jilin, China.
polygonaticola Z.Y. Zhang & W.Q. Pu, in Zhang, Liu, Wei &
He, Plant Diseases and Their Control: 105. 1998.
Holotype: China, Jiangxi, Lushan, on living leaves of Polygonatum
cirrhifolium (Asparagaceae, Liliaceae s. lat.), 6 Oct. 1980, J.Y. Li &
T.Y. Zhang, No. 41551 (MHYAU 03949).
Ill.: Zhang et al. (1998c: 106, ig. 3), Zhang et al. (2003: 146, ig.
98; pl. 13, ig. 5).
Original diagnosis (Zhang et al. 1998c): Maculata amphigena,
ellipsoidea, grisea, marginae atrobrunnea, protrudenta, 5 × 3 mm.
Conidiophora solitaria, erecta, simplicia, septata, pallide brunnea,
65–167 × 3.1–5.1 μm, parte fertii sympodialiter prolifera geniculata
denticulata, sinuolata. Ramoconidia continua vel 1 septata, laevia,
cicatricia vel denticulata praedita, pallide brunnea, 5.7–20.6 ×
5.1–5.1 μm. Conidia catenata, continua, ellipsoidea, laevia, pallide
brunnea, cicatricia inconspicua, 3.1–7.7 × 2.6–3.9 μm.
Notes: Zhang et al. (2003) cited three hosts, viz., Polygonatum
cirrhifolium, P. cyrtonema (China, Hubei) and P. sibiricum (China,
Yunnan). The conidiophores of C. polygonati, described on
Polygonatum sp. from Ireland, are solitary or fasciculate, typically
monilioid, especially swollen at the conidiogenous loci, wider, 9–10
μm wide in the narrow parts, 15–18 μm where swollen; and the
conidia are solitary or formed in short chains, 1–3-septate, longer
and, above all, wider, (35–)40–70 × 18–24 μm (David 1997).
polytrichorum Reichardt, Verh. K.K. Zool.-Bot. Ges. Wien 27:
844. 1877, 1878.
Type: Austria, Tobelbad, near Graz, on Polytrichum formosum
(Polytrichaceae), Sep. 1875, H.W. Reichardt.
Original diagnosis (Reichardt 1878): Hyphae in stupam e ferrugineo
nigricantem contextae, longissimae, lexuosae, irregulariter
the genuS Cladosporium
ramosae, cylindricae, apice obtusae, 0.003 mm crassae, fuscae,
pachydermae, septatae, articulis diametro transversali terquinquies
longioribus. Conidia hypharum articulis supremis lateraliter in
ramulis brevibus conicis, 0.004–0.006 mm longis, 0.003 mm latis
insidentia, mox decidua, unicellularia, sub-reniformia, 0.006–0.009
mm longa, e fusco nigricantia, cytiodermatae crasso, tuberculato.
An status conidiophorus Lizoniae emperigoniae Ces.?
Notes: This species is probably not a Cladosporium.
potebniae Pidopl. & Deniak, Mikrobiol. Zhurn. 5(2): 189, 194.
1938, as “potebnjae”, nom. inval.
Type: Ukraine, from rotting fruit of Malus sp. (Rosaceae).
Lit.: Pidoplichko (1953: 270).
Ill.: Pidoplichko & Deniak (1938: 189, ig. 6).
Notes: Type is probably not preserved. Status remains unclear.
profusum Desm. ex Sacc., Syll. fung. 10: 602–603. 1892.
Type: France, Desmazières, Pl. Crypt. N. France 755 (e.g., PC).
≡ Cladosporium profusum Desm., Pl. Crypt. N. France, No. 755. 1836,
nom. nud.
≡ Cladosporium profusum Desm., in Rabenhorst, Fungi Eur. Exs., Cent.
6, No. 578. 1863, nom. nud.
Lit.: Saccardo (1886: 370, 1892: 602), Lindau (1907: 832),
Gonzáles-Fragoso (1927: 211).
Original diagnosis (Saccardo 1892): Hyphis fasciculatis, nodulosis,
septatis, fuscis, sursum pallidioribus, erectis, 6–7 μm crassis;
conidiis apice hypharum v. nodulis insertis, ovoideis, magnitudine
variis, continuis, uni-biseptatis, guttuligeris, 18–24 × 5 μm, pallidis.
Notes: Lindau (1907): “...Ebensowenig war es mir möglich
festzustellen, wo Rabenhorst die Diagnose veröffentlicht hat und
ob dies überhaupt geschehen ist. Woher Saccardo die Diagnose
hat, ist mir nicht bekannt. Cooke hat in seinem zitierten Aufsatz nur
die Abbildung, nimmt aber im Text auf die Art keinen Bezug. Am
besten läßt man sie vielleicht ganz fort”.
Notes: Gonzáles-Fragoso (1927: 211) provided a description
and considered this species to be very close to C. herbarum.
Rabenhorst, Fungi Eur. Exs. 578 (B, HAL, HBG) contained only
C. herbarum, and the type of C. profusum f. (var.) robustior also
proved to be the latter species.
prunicola McAlpine, Fungus Dis. Stone-fruit Trees Austral.:
100. 1902, as ‘prunicolum’.
Type: Australia, Victoria, Armadale near Melbourne, on partly
decayed leaves of Prunus armeniaca (= Armeniaca vulgaris)
(Rosaceae), Dec. 1899.
Lit.: Saccardo (1906: 575), Braun (2001: 53).
Ill.: McAlpine (1902: Figs 89, 90).
Notes: Type material is not preserved in VPRI, but description is
close to C. macrocarpum (J. Cunnington, in litt.) (Braun 2001).
psidiicola J.M. Yen, Bull. Trimestriel Soc. Mycol. France
95(3): 188. "1979" 1980.
Type: Hong Kong, Tai-Yuan-Yu-Tsun, Hsin-Chiai, Kowloon, on
living leaves of Psidium guajava (Myrtaceae), 13 Nov. 1971, Jomin Yen, No. 71334 (LAM).
Ill.: Yen (1980: 187, ig. 2).
Original diagnosis (Yen 1980): Maculis irregulariter conluentibus,
pallide brunneis. Hyphis internis subepidermicis vel intra cellulis
epidermicis, pallide brunneis, ramosis, septatis, 3–7 μm diam.
Conidiophoris plerumque fasciculatis, raro solitariis, simplicibus vel
ramosis, pallide brunneis, erectis vel lexuosis, 0–5 septatis, 0–3
geniculatis, ad apicem rotundatis et cicatricibus ornatis, 15–70 ×
www.studiesinmycology.org
3–5(–6) μm. Hyphis externis, hypophyllis, leviter repentibus, pallide
brunneis, septatis, ramosis, 3–5(–6) μm crassis. Conidiis catenatis,
ellipsoideis, fusiformibus vel cylindraceis, pallide brunneis, rectis vel
leviter curvatis, 0–2(–3) septatis utrinque rotundatis et cicatricibus
atrobrunneis ornatis, 5–28 × 3–6 μm.
Notes: Type material was not available for a re-examination.
punctatum (Sacc.) Sacc., Syll. fung. 4: 355. 1886.
Type: France, Rouen, Malbranche, on Euonymus japonicus
(Celastraceae).
Basionym: Cladosporium compactum *punctatum Sacc., Michelia
2(7): 363. 1881.
Lit.: Saccardo (1886: 359).
Original diagnosis (Saccardo 1886): Acervulis gregariis
punctiformibus; hyphis brevibus subcontinuis, 30 × 4 μm; conidiis
acrogenis oblongis, 15 × 5 μm, saepius continuis, olivaceis.
Notes: “Afine Cl. subcompacto Sacc.” (Saccardo 1886). Type
material could not be found in herb. Saccardo.
punctulatum var. xylogenum Fairm., Proc. Rochester Acad.
Sci. 6: 131. 1922.
Type: USA, New York, Lyndonville, on the outside of a cigar box
exposed to damp weather, 14 Dec. 1920, C.E. Fairman (associated
with Epicoccum agyrioides).
Original diagnosis (Fairman 1922): Hyphae long, lexuose,
septate, often containing a series of globose oil drops, 5–7 μm in
width: spores ellipsoid, 1–3-septate, brown, minutely punctulate
roughened, 10–20 × 7–8 μm.
Notes: Type material could not be found.
pyriforme Reichert, Bot. Jahrb. Syst. 56: 721. 1921, as
“pyriformum”.
Type: Egypt, near Bulak, on cladodes of Opuntia icus-indica
(Cactaceae), 1822/25, Ehrenberg (B).
Lit.: Saccardo (1972: 1338, as “piriforme I. Reichert”).
Ill.: Reichert (1921: tab. 4, ig. 4).
Original diagnosis (Reichert 1921): Caespitulis confertis, atris
pyriformibus, sub epidermide luxuriantibus, 150–200 μm longis, 60–
120 μm latis; hyphis fertilibus fasciculatis, rectis, interdum ramosis,
septatis, fuscescentibus, 120–170 μm longis, 2–4 μm latis; conidiis
terminalibus, elongatis, ellipsoideis, 2-, raro 3-septatis, vel catenulatis,
fuscescentibus, mox deciduis, 5–8 μm longis, 3–5 μm latis.
Notes: Status remains unclear.
qinghaiense T. Zhang & Z.Y. Zhang, Proceedings of Phytopathological Symposium Organized by Phytopathology
Laboratory of Yunnan Province 2: 285. 1998, as “qinghaiensis”.
Holotype: China, Qinghai, Huzhu, on Pisum sativum (Fabaceae), 3
Aug. 1989, H. Li & T.F. Li, No. 087 (MHYAU 03925).
Ill.: Zhang et al. (1998b: 286, ig. 1), Zhang et al. (2003: 148, ig.
100).
Original diagnosis (Zhang et al. 1998b): Maculis irregularibus,
lavidis, 0.2–0.5 mm diam. Coloniis olivaceis hypophyllis. In CMAcultura in 10 diebus ad 25 ºC coloniis 26 mm diam., atro-olivaceis,
effusis vel apiculatis, aerobiis hyphis amplis, myceliis immersis vel
supericialibus. Conidiophoris plerumque fasciculatis, raro ramosis,
rectis vel lexuosis, 0–3-septatis, laevis, pallide brunneis, ad
apicem cicatricibus ornatis, 60–120 × 4.0–5.4 μm. Conidiis breviter
catenatis, ellipsoideis vel late ellipsoideis, brunneis, verruculosis,
0–3-septatis, cicatricibus ornatis, 10.8–21.6 (15.6) × 5.4–9.5 (5.8)
μm.
361
BenSch et al.
ramulosum Reissek, Sitzungsber. Kaiserl. Akad. Wiss.,
Math.-Naturwiss. Cl. 7(2): 336. 1851.
Type: Austria, on pollen of Pinus sylvestris (Pinaceae).
Lit.: Saccardo (1886: 370, 1913a: 1370), Lindau (1907: 833).
Original diagnosis (Reissek 1851): Entsteht aus den Inhaltskörnern
des Pollens von Pinus sylvestris, wenn er auf das aufgerissene
Parenchym krautartiger Planzentheile gestreut wird, oder
in Infusionen sich vorindet. Die Entwicklung im ersten Falle
vollständig, im zweiten theilweise beobachtet. Sie erfolgt, indem
sich die Inhaltskörner vergrössern, nach Aulösung der Pollenzelle
frei werden, die äusserste Schichte derselben sich zur Membran
differenziert, und die Zelle in einen ästigen, sporentragenden
Schlauch auswächst.
Notes: Reissek (1851): “(C. entoxylinum Corda var. ?)”. “An
diversum a Cladosporio ramuloso Desm.?” (Saccardo 1886).
“Species omnino dubia, cfr. Lindau l.c.” (Saccardo 1913a). Type
material could not be traced. Status remains unclear.
raphanicola Opiz, Seznam: 117. 1852, nom. nud.
Type: Czech Republic, on Raphanus ? (Brassicaceae).
Lit.: Saccardo (1886: 370, as “raphanicolum”), Lindau (1907: 832),
Kupka (1918: 156).
Notes: Type is not preserved at PRM. Kupka (1918): “Von dem
variablen C. herbarum kaum verschieden...“.
sambuci Brunaud, Champ. Charente-Infer. 1892: 38. 1892.
Type: France, Saintes, on leaves of Sambucus nigra (Adoxaceae).
Lit.: Saccardo (1895: 620).
Original diagnosis (Saccardo 1895): Maculis brunneis; acervulis
sparsis v. conluentibus; hyphis olivaceis, septatis, interdum
ramosis; conidiis subfusiformibus, olivaceis, primitus continuis,
dein 1–3-septatis, 15–30 × 5–8 μm, septis haud constrictis.
Notes: Zhang et al. (2003) reported a collection on Sambucus
williamsii from China determinated as Cladosporium sambuci, but
the conidia of the Chinese collection were described to be much
narrower, 2.6–5.1 μm.
savastani Carbone, Atti Ist. Bot. Univ. Pavia, Ser. 2, 14: 322.
1914.
Type: Italy, Pavia, isolated from sausage [“in botulis (Salame
crudo)”].
Lit.: Saccardo (1931: 799).
Original diagnosis (Carbone 1914): Effusum, atro-olivaceum.
Hyphis sterilibus crassis, quasi torulosis, septatis, dilute olivaceis,
7–10 μm diam; fertilibus erectis, simplicibus vel ramosis, brunneis,
septatis, 100–350 × 4–6 μm; conidiis in hyphis et in ramis insertis,
acropleurogenis, ovalibus vel oblong-ellipticis, vel pyriformibus,
uniseptatis vel (saepius) continuis, 8–20 × 4–7 μm.
Notes: Type material unknown, probably not preserved. Status
remains unclear.
secedens Fr., Summa veg. Scand. 2: 499. 1849.
1832.
Type: USA, Pennsylvania, Bethlehem, on fallen leaves of Fraxinus
sp. (Oleaceae), No. 2606 (PH 1020415).
Lit.: Saccardo (1886: 360).
Original diagnosis (Schweinitz 1832): C. loccis simplicibus, nigris,
fere omnino septatis, ceterum in aversa pagina tam approximates,
ut sistent maculam parvam, tenuissimum. Ceterum erectis,
minutissimis. Sporidiis adhaerentibus, satis crebris, et ad basin
loccorum hinc inde disperses, concoloribus.
Notes: The taxonomic status of this species is quite unclear since
the type material is too meagre for a inal conclusion. It was not
possible to ind suficient fructiication for a re-evaluation of this
species. The original description (Schweinitz 1832) is too brief and
non-informative for any conclusions about the status of this fungus.
smilacis (Schwein.) Fr., Syst. mycol. 3(2): 369. 1832.
Syntypes: USA, Salem, Bethlehem, in the epidermis of Smilax
rotundifolia (Smilacaceae), (PH 01020424, 01020425).
Basionym: Dematium smilacis Schwein., Syn. fung. Carol. sup.:
102. 1822.
Lit.: Saccardo (1886: 367).
Original diagnosis (Fries 1832): Fasciculatum, erumpens, incanum,
ibris, contiguis hyalino-pellucidis, apice sublobatis, stromate junctis.
Notes: Status remains unclear. No Cladosporium found, type
material in bad condition. Zhang et al. (2003) cited a collection on
Smilax china from Shaanxi, China.
solutum Link, Sp. pl., 6(1): 39. 1824.
Type: Egypt, near Sjut, on stems of Hibiscus esculentus (≡
Abelmoschus esculentus) (Malvaceae), Oct., Ehrenberg.
≡ Cladosporium herbarum b solutum (Link) Rabenh., Krypt.-Fl. 1: 113.
1844.
≡ Cladosporium herbarum var. solutum (Link) Sacc., Syll. fung. 4: 351.
1886.
Lit.: Prasil & de Hoog (1988: 53, as “C. herbarum var. solutum”).
Original diagnosis (Link 1824): Cl. thallo effuso, loccis dense
aggregatis obscure viridibus leviter adnatis, sporidiis globosis et
ovalibus concoloribus.
Notes: Authentic material is not preserved in herb. B, so the identity
of the species cannot be established (Prasil & de Hoog 1988). In
Oudemans (1923), Ricinus communis (Euphorbiaceae) is given as
host.
stenosporum Berk. & M.A. Curtis, Grevillea 3(27): 107. 1875.
Type: USA, South Carolina, on Stylosanthes sp. (Fabaceae), No.
2067 and on leaves of Malus sp. (Rosaceae), No. 2529 (not in
herb. K).
Lit.: Saccardo (1886: 352).
Original diagnosis (Berkeley 1875): Floccis continuis deorsum
fuscis; sporis angustis.
Flocci unbranched, darker below, slender, articulated, springing
from a decumbent mycelium; spores oblong, narrow.
Notes: Bubák (1916) examined material of Acrotheca dearnessiana
Sacc. sent to him by J. Dearness and thought it to be Cladosporium
stenosporum. Acrotheca dearnessiana is a synonym of Fusicladium
pyrorum (Schubert et al. 2003; Crous & Braun 2003). Zhang et
al. (2003) reported and illustrated a collection determinated as
C. stenosporum from Zhejiang, China on Pyrus calleryana.
Type: Scandinavia, on rotten Sterea (= Stereum).
Lit.: Saccardo (1886: 368).
Original diagnosis (Fries 1849): Caespites formut irregulars, effuses
densos, fuscoolivaceos, ibris creclis simplicibus, aequalibus,
undique septatis pellucidis, sporidiis 2, 5-septatis, septis dein
secedentibus.
Notes: Type material not found. Status remains unclear.
stercorarium Corda, Icon. fung. 1: 14. 1837.
simplex Schwein., Trans. Amer. Philos. Soc., N.S., 4(2): 277.
Type: Czech Republic, on bird dung.
Lit.: Saccardo (1886: 369), Lindau (1907: 831).
362
the genuS Cladosporium
Fig. 399. Cladosporium stromatum (B 700006714). Conidiogenous cells and conidia. Scale bar = 10 µm. K. Bensch del.
Ill.: Corda (1837: tab. 3, ig. 205).
Original diagnosis (Corda 1837): Acervulis subeffusis, olivaceis,
glaucescentibus; loccis erectis, simplicibus vel ramosis,
pauciseptatis, curtis, olivaceo-cinereis; sporis simplicibus oblongis,
concoloribus, in loccos tenuissimos concatenatis.
Notes: Type material is not preserved in herb. PRM.
stercoris Speg., Anales Mus. Nac. Buenos Aires, Ser. 2, 6:
338. 1899.
Type: Argentina, Parque de La Plata, on old rabbit dung, May
1888.
Lit.: Saccardo (1902: 1059), Farr (1973: 251).
Original diagnosis (Spegazzini 1899): Glomerulosum sparsum
viridi-olivaceum, hyphis decumbentibus subtorulosis, conidiis
ellipsoideis didymis. Acervuli pulvinulati crassiusculi compactiusculi
hemisphaerici v. elongati (2–5 mm diam.) subvelutini intense
virescente-olivacei: hyphae repentes longiusculae lexuosae laxe
ramulosae dense intertextae graciles (5 μm crass.) chlorinae crebre
septulatae, ad septa plus minusve constrictae, articulis subcuboideis,
hinc inde papillato-apiculatae; conidia pleurogena ellipsoidea v.
obovata (12–15 μm long = 5–6 μm crass.) 1-septata, ad septum
constrictula, loculo infero saepius minore, laevia chlorina.
Notes: No type material at LPS.
straminicola Pidopl. & Deniak, in Pidoplichko, Gribnaya Flora
Grubykh Kormov: 269. 1953, nom. inval.
Type: Ukraine, on straw and on hay.
Ill.: Pidoplichko (1953: 269, ig. 70).
Notes: Type material probably not preserved. Status remains
unclear.
strobilanthis H.J. Lu, Y.L. Liu & Z.Y. Zhang, Mycosystema 22:
49. 2003.
Holotype: China, Shaanxi Prov., Xian, on living leaves of
Strobilanthes cusia (Acanthaceae), 29 Aug. 1989, T.F. Li & H. Li
(MHYAU 07908).
Original diagnosis (Lu et al. 2003): Maculae amphigenae,
suborbiculares, ellipticae, griseo-viriduae vel pallide brunneae,
www.studiesinmycology.org
margine atro-brunneae, 0.4–10 mm diam. Caespituli saepe
hypophylli, punctiformes, griseo-atrobrunnei. Mycelium immersum.
Conidiophora solitaria, erecta, 1–3-septata, pallide brunnea.
Cicatrices conidiales conspicuae, fuscae, 5.1–139.8 × 2.6–7.7
μm. Ramoconidia 0–1-septata, olivacea, ad apicem denticulata,
cicatricibus, 11.8–18.5 × 3.1–3.9 μm. Conidia catenata, longe
ellipsoidea, fusiformia, non-septata, pallide olivacea, hila leniter
incrassata.
stromatum Preuss, Deutschl. Fl. 3(26): 25. 1848. Figs 399,
400.
Type: Germany, Hoyerwerda, on wood of Pinus sp. (Pinaceae),
Preuss (B 700006714).
Lit.: Saccardo (1886: 352, 355), Lindau (1907: 811), Ferraris (1912:
339), Oudemans (1919: on Eutypa lejoplaca, 1920: on Juglans
regia, 1921: on Acer campestris), Heuchert et al. (2005: 57).
Ill.: Preuss (1848: tab. 13).
Original diagnosis (Preuss 1848): Stroma communiter convexum;
loccis ramosis, lanosis, irregulariter septatis, subintricatis fuscis;
sporis apice concatenatis copiosis, globosis, simplicibus, vel di- vel
polydymis, oblongis, concoloribus.
In vivo: On wood. Colonies small, subcircular, pustulate, becoming
conluent, dense, caespitose, pale grey-brown to dark-brown,
velvety. Mycelium supericial, hyphae branched, septate, 1.5–4 μm
wide, subhyaline, thin-walled, somewhat dimorphic, becoming wider
and more thick-walled, 4.5–8 μm wide, more or less constricted
at septa, smooth or almost so to somewhat rough-walled, thickwalled. Conidiophores solitary, very short, as lateral outgrowths
of plagiotropous, wider, thick-walled hyphae. Conidiogenous cells
integrated, terminal, with a single or few apical conidiogenous loci,
protuberant, 1–1.5(–2) μm diam. Conidia catenate, in unbranched
or branched chains, globose, subglobose, ovoid, broadly ellipsoid
to oblong or somewhat irregular, 3–35 × 3–9 μm, 0–3-septate, often
distinctly constricted at septa, subhyaline or pale yellowish, smooth
or almost so, thick-walled, apex rounded with up to three apical
hila, hila protuberant, conspicuous, 1–1.5(–2) μm diam, thickened
and darkened-refractive.
Notes: The status of C. stromatum remains unclear. SEM
examinations showed Cladosporium-like conidiogenous loci and
363
BenSch et al.
Fig. 400. Cladosporium stromatum (B 700006714). A–B. Details of the scar structure on conidia. Scale bars = 1 (B), 5 (A) µm.
scars (see Fig. 400), but the morphology is quite distinct from
Cladosporium. It requires further examinations and molecular data
are urgently needed to clarify the status of this species.
subcompactum Roum. & P. Karst. → compactiusculum.
subcompactum Sacc., Syll. fung. 4: 361. 1886.
Type: France, Rouen, Letendre, on dead stems of Consolida ajacis
(≡ Delphinium ajacis) (Ranunculaceae).
≡ Cladosporium compactum Sacc., Michelia 2(6): 127. 1880, nom. illeg.,
non C. compactum Berk. & M.A. Curtis, 1875.
Lit.: Lindau (1907: 825), Ferraris (1912: 344, 1914: 884), Oudemans
(1921), Gonzáles-Fragoso (1927: 203).
Original diagnosis (Saccardo 1886): Caespitulis erumpentibus,
compactiusculis, olivaceis, 1/3–1/2 mm. diam.; hyphis fasciculatis,
simplicibus furcatisve, eseptatis, 70–90 × 6–7 μm, melleo-olivaceis;
conidiis rhombeo-fusoideis, 10–14 × 6–7 μm, 1-septatis, non
constrictis, concoloribus.
Notes: Type could not be found, probably not preserved.
subfusoideum McAlpine, Fungus Dis. Citrus Trees Austral.:
79. 1899.
Type: Australia, Victoria, Wandin Yallok, on fruits of Citrus medica
(Rutaceae), Sep. 1898 and New South Wales, Parramatta, Feb.
1899, Cairnes.
Lit.: Saccardo (1902: 1058).
Ill.: McAlpine (1899: pl. 5, igs 21–22).
Original diagnosis (McAlpine 1899): Greyish-brown scab,
overspreading green or yellow lemon, mostly on one side, cracking
and becoming covered in patches with dusky layer. Hyphae
ascending, dark olive, septate, very sparingly branched, average
4 μm thick. Conidia produced at apex, similarly coloured to hyphae
or paler, 1–3-septate, not constricted at septa, smooth, fusiform or
subfusoid, 12–15 × 3.5–4 μm.
Notes: Types are lost, no specimen in VPRI. “Type details agree
with Diplodia citricola McAlpine (in part)” (personal communication
with J. Cunnington / I. Pascoe).
sycophilum Farneti, Atti Ist. Bot. Univ. Pavia, Ser. 2, 8: 517.
1904, as “sicophilum”.
Type: Italy, Pavia, on living fruits of Ficus carica (Moraceae).
Lit.: Saccardo (1906: 576), Lindau (1907: 821), Ferraris (1912: 347).
364
Original diagnosis (Farneti 1904): Caespitulis solitariis,
sparsis, minutis, inaequalibus, bruneis; hyphis geniculatis, vel
assurgentibus, simplicibus, septatis, ad septa constrictis, bruneis
in acervulum torulosum aggregatis, 35–80 × 4.5 μm diam.; conidiis
acrogenis, oblongis, uniseptatis, dilute fuscescentibus, diaphanis,
5–10 × 4 μm diam.
Notes: In Oudemans (1920) Morus alba is given as a further host.
Type could not be traced.
syringae Montem., Riv. Patol. Veg., Ser. 2, 1915: 226. 1915.
Type: Italy, Montubeccaria, Pavia, on leaves of Syringa vulgaris
(Oleaceae).
Lit.: Saccardo (1931: 793).
Original diagnosis (Saccardo 1931): Amphigenum; caespitulis
sparsis, brunneis, in stromate epidermico vel subepidermico
insertis; hyphis fasciculatis, simplicibus, lexuosis, septatis,
fuscidulis, summo subhyalinis, 60–80 × 3–4 μm; conidiis brunneis
vel olivaceis, 1–2-septatis, cylindricis, levibus, 15–20 × 3 μm.
tabaci Oudem., Beih. Bot. Centralbl. 11: 538. 1902.
Type: Netherlands, Bussum, on decaying leaves of Nicotiana
tabacum (Solanaceae), Aug. 1901, C.J. Koning (L).
= Cladosporium nicotianae Oudem., Ned. Kruidk. Arch., Ser. 3, 2(3): 769. 1902.
Lit.: Saccardo (1906: 576), Lindau (1907: 829).
Original diagnosis (Oudemans 1902a): Hyphis caespitosis,
erectis, divergentibus, simplicibus, lexuosis, subnodosis, septatis,
avellaneis (Sacc. Chrom. No. 7). Conidiis acrogenis, ellipticis, 8 ×
5–4 μm, primo hyalinis, denique avellaneis, 1-septatis.
Specimen examined: Cuba, Prov. Remedios, Antilles, on Nicotiana tabacum, Dr. C.
Preißecker, Krypt. Exs. 1628 (B 700006719, HBG, M).
Notes: The type of C. nicotianae [Netherlands, Amerongen, on decaying
leaves of Nicotiana tabacum, Jul. 1901, C.J. Koning (L).] only consists
of two drawings, and the description is rather brief [(Oudemans 1902b):
Hyphes en touffes médiocrement denses, en partie diffuses, en partie
dressées, simples, lexueuses, à peine noueuses couleur noisette
(Sacc. No. 7), 150–170 × 20 μm, terminées par une conidie elliptique,
8 × 4–5 μm, d’abord hyaline, plus tard couleur noisette, ordinairement
1-septée.], and the drawings are so poor that this species was regarded
as doubtful [a nomen dubium] (de Vries 1952).
A single collection from herb. B determinated as C. tabaci has
been examined but proved to be quite distinct from the original
description given above by having hyaline scolecosporous conidia
and typical conspicuously thickened and darkened, planate
the genuS Cladosporium
conidiogenous loci and hila belonging to the Cercospora-type. The
fungus examined belongs to Cercospora apii s. lat.
tenerrimum Link, Sp. pl. 6(1): 41. 1824.
Type: Germany, Berlin, on rotting bark, Link (B 700006720).
Lit.: Saccardo (1886: 355), Lindau (1907: 831).
Original diagnosis (Link 1824): Cl. thallo effuse, loccis dense
aggregatis albis, sporidiis globosis.
Notes: Type material contains various saprobic hyphomycetes, but
no true Cladosporium has been found.
tetrapanacis D.X. Wu & Z.Y. Zhang, Mycosystema 22: 48.
2003.
Brusca nas plantas cultivacas. Nos tecidos atacados se nota um
mycelio relativamente grosso, primeiro hyalino, depois pardo,
ramiicado, septado, que invade os elementos do parenchyma
elaborante e do respiratorio, envolve e atravessa as cellulas e
inalmente emitte touceiras de conidiophoros (ig. 4r) formadas por
3 a 15 conidiophoros, divergentes, sinuosos ou rectos, septados
fuliginosos, tendo um crescimento apical muito accentuado,
ligeiramente reentrantres em relação dos septos. No ápice dos
conidiophoros se formam, agamicamente, os conidios. Elles são
cylindricos ou ovaes, com ápices arredondados, muito ligeiramente
curvados (ig. 4a), hyalinos (13 a 17.5 × 4.5 a 8 microns), alguns
continuos, outros uniseptados, com conteúdo homogeneo e parede
relativamente grossa. Germinam facil e rapidamente (ig. 5n, s, g)
produzindo um ou dois tubos de germinação, um da cada loculo,
primeiro continuo, depois setado, ramiicado.
Holotype: China, Shaanxi Prov., Xian, on living leaves of Tetrapanax
papyrifer (Araliaceae), 29 Aug. 1989, T.F. Li (MHYAU 07906).
Ill.: Wu & Zhang (2003: 49, ig. 1).
Original diagnosis (Wu & Zhang 2003): Maculae amphigenae,
suborbiculares, brunneae vel griseo-albidae, centro griseobrunneae, 0.8–5.5 mm diam., margine pallide lavido-ochraceae.
Caespituli hypophylli, griseo-fusci. Conidiophora solitaria, erecta,
septata, geniculata, atro-brunnea, 35.1–75.6 × 2.7–4.1 μm.
Ramoconidia 0–1-septata, pallide brunnea, ad apicem denticulata
vel conspicue cicatricosa, 6.8–21.6 × 3.2–4.9 μm. Conidia catenata,
ellipsoidea vel fusiformia, 0(–1)-septata, sporarum cicatrices
visibiles, 6.5–13.5 × 3.2–4.9 μm.
Type: Scandinavia, on wood of Quercus (Fagaceae).
Lit.: Saccardo (1886: 354).
Original diagnosis (Fries 1849): Dignoseitur ibris elongatis tenellis,
intricatis, lexuosis, opacis, remote septatis, sporidiis globosis, raro
septatis, sed in ramulos concatenatis, dein liberis ibris inspersis et
sic Dematio afine.
Notes: Type material could not be found. Status remains unclear.
teucrii Y.L. Liu & Z.Y. Zhang, Plant Diseases and Their
Control: 101. 1998.
transchelii Pidopl. & Deniak, Mikrobiol. Zhurn. 5(2): 188, 194.
1938, nom. inval.
Holotype: China, Hubei, Wuchang, on living leaves of Teucrium
viscidum (Lamiaceae), 24 Sep. 1980, J.Y. Li & T.Y. Zhang, No.
41007 (MHYAU 03954).
Ill.: Liu & Zhang (1998: 102, ig. 1), Zhang et al. (2003: 168, ig. 116;
pl. 13, ig. 3).
Original diagnosis (Liu & Zhang 1998): Foliorum macula
amphigena, subcircularis, brunnea vel pallide olivaceo-grisea,
marginatis fuligenea prominula, hypophylla fulva, 2–3 mm diam.
Conidiophora solitaria, erecta, 50–89 × 4.3–6.9 μm. Cellulosa
conidiogena in conidiophora incorporta, sympodiala, cicatricia
conspicuis. Ramoconidia continua, laevia, pallide brunnea, apice
denticulata, 8.2 × 3.1 μm. Conidia catenata, ellipsoidea, continua,
pallide brunnea, sporarum cicatricia visibilia, 3.1–9.8 × 2.6–2.8 μm.
theobromicola Av.-Saccá, Bol. Agric. (São Paulo) 21: 59.
1920, as “theobromicolum”.
Type: Brazil, on Theobroma cacao (Malvaceae).
Ill.: Averna-Saccá (l.c.: igs 3–5).
Original diagnosis (Averna-Saccá, l.c.): Nas folhas atacadas (ig.
3, a) primeiramente se nota o empardecimento do ápice, que, aos
poucos, seguindo as margens, attinge até 2/3 da lamina; raramente
chega até á base. Estas manchas augmenttam e mudam de côr,
tendo no im uma coloração cinzenta ou branca. São quebradiças
e cobertas por uma intensa eflorescencia granulosa, ina,
preta, formada pelos orgãos de fructiicação do fungo. As folhas
assim atacadas, estando em ambiente humido, mostram uma
eflorescencia cotonilhosa, branca, devido á intensa formação dos
esporos. As folhas icam, durante algum tempo, presas aos ramos,
mas depois se desarticulam e cáem. Como se vê, o Cladosporium
em questão poderá produzir ao cacaueiro graves prejuizos. No caso
em exame a sécca apical descendente das folhas e o concomitante
desenvolvimento do Cladosporium são frequentes nas plantas já
atacadas pela gommose, ao que conirma ainda uma vez a theoria
de Comes sobre as causas que determinam os phenomenos da
www.studiesinmycology.org
tortuosum Fr., Summa veg. Scand. 2: 499. 1849.
Type: Ukraine, from stalks of Zea mays (Poaceae).
Lit.: Pidoplichko (1953: 273).
Ill.: Pidoplichko & Deniak (1938: 188, ig. 5).
Notes: Type material probably not preserved. Status remains
unclear.
transchelii var. semenicola (Pidopl. & Deniak) Pidopl. & Bilai,
in Pidoplichko, Gribnaya Flora Grubykh Kormov: 274. 1953,
nom. inval.
Type: Ukraine, on grains of oats.
≡ Cladosporium viridiolivaceum var. semenicola Pidopl. & Deniak ?
Notes: Type material probably not preserved. Status is unclear.
It is unknown where the authors published this variety of
C. viridiolivaceum Pidopl. & Deniak.
transchelii var. viridi-olivacearum (Pidopl. & Deniak) Pidopl.
& Bilai, in Pidoplichko, Gribnaya Flora Grubykh Kormov: 274.
1953, nom. inval.
Type: Ukraine, isolated from rotting apples (Malus sp., Rosaceae).
Basionym: Cladosporium viridiolivaceum Pidopl. & Deniak,
Mikrobiol. Zhurn. 5(2): 187, 194. 1938, as “viridi-olivaceum”, nom.
inval.
Ill.: Pidoplichko & Deniak (1938: 187, ig. 4).
Notes: Type material probably not preserved. Status remains
unclear.
tuberculatum Fr., Summa veg. Scand. 2: 499. 1849.
Type: Scandinavia, on ostioli of Cytospora leucosperma
(anamorphic fungus).
Original diagnosis (Fries 1849): C. tuberculatum sistit minuta,
hemisphaerica tubercula, colorae griseofusco, ibris tenellis curtis
pellucidis sursum septatis, sporidiis simplicibus l. uniseptatis.
Notes: No material seen; status remains unclear.
365
BenSch et al.
typharum f. minor Brunaud, Bull. Soc. Bot. France 36: 340.
1889.
Type: France, Rochefort, on leaves of Typha latifolia (Typhaceae).
Lit.: Saccardo (1899: 1081), Oudemans (1919).
Original diagnosis (Brunaud 1889): Conidies oblongues, 1-septées,
olivacées, à deux gouttelettes, long. 15–16, larg. 8.
Notes: Type material could not be found.
ulmariae Grognot, in Roumeguère, Fungi Sel. Gall. Exs.,
Cent. XXXVII, No. 3697. 1886.
Type: France, on Spiraea ulmaria (= Filipendula ulmaria subsp.
ulmaria) (Rosaceae), Roumeguère, Fungi Sel. Gall. Exs. 3697
(e.g., FH).
Lit.: Saccardo (1895: 621).
Notes: Doubtful species. A mixture of several saprobic
hyphomycetes, incl. Cladosporium (some conidia agree with
those of C. herbarum). Large, expanded stromata are developed.
Most conidiophores are broken or without any scars, some
conidiophores are Periconiella-like and a few are subdenticulate.
The original description is very brief, only describing ‘brown,
cylindrical, 3-septate conidia’. It is imposssible to interprete what
Roumeguère intended to describe.
umbrinum Fr., Syst. mycol. 3(2): 372. 1832.
Type: France, Montagne, on Agaricus olearius (Agaricales).
= ? Cladosporium herbarum (Pers. : Fr.) Link, Ges. Naturf. Freunde Berlin
Mag. Neuesten Entdeck. Gesammten Naturk. 7: 37. 1816.
Lit.: Saccardo (1886: 369), Lindau (1907: 807), Oudemans (1919),
Heuchert et al. (2005: 57).
Original diagnosis (Fries 1832): C. umbrinum, caespitibus effusis,
contiguis tenuibus velutinis umbrinis, ibris brevibus lexis, sporidiis
glomeratis. Densa et contigua sericea tela fungos obducit forte huic
debetur phosphorescenti Ag. olearius. Fibrae contiguae, simplices
l. ramosae, colore peculiari olivaceo-umbrino insignes. Sporidia
conglomerata ut in Botrytide, sed certe hujus generis est species.
Synonymon dubium, ill. Auctor ipse de genere dubiat. Ad Agaricum
olearium in Gallis meridionali, Montagne (v. s.).
Specimen examined: Germany, original label: “Botrytis pulvinata, Persoon”, ex
herb. Link (B 700006764, holotype of Botrytis pulvinata Link).
Notes: In the original description of C. umbrinum, Fries (1832)
cited Botrytis pulvinata as possible synonym (with question mark).
Saccardo (1886) listed C. umbrinum, but cited Botrytis pulvinata as
synonym without question mark. Type material of B. pulvinata has
been examined and proved to be synonymous with Cladosporium
herbarum. Cladosporium umbrinum is very probably a synonym
of the latter species as well. Saccardo (1886) and Lindau (1907)
recorded C. umbrinum on Pleurotus olearius, fruit bodies of other
mushrooms and honeycombs of wasps from Belgium, France and
Germany. Agrocybe praecox was listed as host of this species
from Armenia and the Ukraine (Osipjan 1975). On the web-side
“biodiversity.ac.psiweb.com”, C. umbrinum has been listed from the
Ukraine on “Agaricales (Fam. indet.)” and Lactarius sp.
uniseptosporum Matsush., Icones
Matsushima Lectorum: 36. 1975.
Microfungorum
a
Type: Japan, Chiba, Kiyozumi Exp. Forest, Univ. Tokyo, on rotten
wood, Oct. 1967 (Matsush. herb. 2147).
Lit.: Ho et al. (1999: 142).
Ill.: Matsushima (1975: pl. 59, ig. 3), Ho et al. (1999: 143, ig. 48).
Original diagnosis (Matsushima 1975): In b/c- et CMA-culturis:
Coloniae tarde crescentes, coactae, olivaceae, margine restrictae,
reversione atrofuscae. Mycelium ex hyphis ramosis, septatis,
366
hyalinis-modice brunneis, 1–3 μm crassis compositum. Conidiophora
solitaria, dense erecta, simplicia, rigida, septata, (20–)40–90 μm
longa, 2.5–3.5 μm crassa, spice sympodialiter prolifera cicatricosa,
sinuolata, inferne brunnea sursum pallide brunnea. Conidia catenas
breves (usque ad 4) simplices vel ramosas formantia, cylindroellipsoidea, 1-septata, laevia, sicca, 5.5–10 × 2.5–3 μm, capitulum
laxum radiatum olivaceum producentia.
Notes: Type material could not be available. Status remains unclear.
urediniphilum Speg., Anales Mus. Nac. Hist. Nat. Buenos
Aires 31: 438–439. 1923.
Holotype: Paraguay, Asunción, Puerto Sajonia, on sori of Uredo
cyclotrauma (Uredinales) on leaves of Zygia latifolia var. communis
(= Pithecolobium caulilorum) (Fabaceae), Oct. 1919, C. Spegazzini
(LPS). Permanent slide: IMI 87163b.
Lit.: Deighton (1969), Saccardo (1972: 1340), Farr (1973: 252),
Sutton (1973: 40), Heuchert et al. (2005: 58).
Ill.: Sutton (1973: 41, ig. 19 B).
Original diagnosis (Spegazzini 1923): Velutinum olivaceum mycelio
in acervulis procurrente, hyphis tenuibus sursum, ad acervulorum
margines praecipue, exertis pluriseptatis, olivaceis, simplicibus
vix lexuosis, articulo supremo obsolete alterneque denticulato,
denticulis conidiiferis: conidia elliptica v. subcylindracea, parva,
utrimque plus minusve rotundata, continua v. 1-septata, laevia,
hyalina v. vix fumosa.
Notes: In additional notes Spegazzini (1923) described the
conidiophores and conidia as follows: Conidiophores 100–200
× 4–5 μm, 4–10-septate, without any constrictions at the septa;
conidia 6–10 × 4–5 μm. Sutton (1973) examined the type material of
this species and deposited a permanent slide at IMI. He described
a species with verruculose surface sculpture, more conspicuous
than in C. gallicola, and he stressed that the type collection was
very meagre, without any intact conidiophores and only few
conidia. Additional specimens of C. urediniphilum are not known,
and the type material is too scarce for a inal conclusion about the
status of this species and its relation to C. uredinicola and other
uredinicolous Cladosporium species.
uvarum McAlpine, Add. fungi vine Australia: 47. 1898.
Type: Australia, on berries dried up and shrunken of Vitis vinifera
(Vitaceae).
Lit.: Saccardo (1899: 1079).
Ill.: McAlpine (1898: igs 72–74).
Original diagnosis (Saccardo 1899): Effusum, olivaceum,
crassiusculum; hyphis sterilibus nodulosis, septatis, ramosis,
lavo-viridulis brunneisque, 4–15 μm cr., hinc inde inlatis; hyphis
fertilibus, atro-virentibus, septatis, apicibus obtusis v. subtenuatis,
pallidioribus, 5.5–6 μm cr.; conidiis polymorphis globosis, ovoideis
v. elongatis v. piriformibus, 10–19 × 4–9 μm, olivascenti-brunneis,
1–2-septatis v. continuis, levibus.
Notes: Type material or any other samples not seen. Status remains
unclear. Zhang et al. (2003) reported the species on Vitis vinifera
from Shaanxi, China.
venturioides Sacc., Nuovo Giorn. Bot. Ital., N.S., 22: 71.
1915.
Type: Malta, Zebbih, on faded or dead branches of Amaranthus
caudatus (Amaranthaceae), Jan. 1914, No. 643.
Lit.: Saccardo (1931: 796).
Original diagnosis (Saccardo 1915a): Caespitulis in maculis
fuscescentibus matricis gregariis, punctiformibus, venturioideis
the genuS Cladosporium
nigris; hypostromate hemisphaerico irregulariter celluloso,
fuligineo, 35 μm diam., hyphis conidiophoris ad hoc radiantibus,
teretiusculis, variae longitudinis, 30–50 × 2.7–3 μm, sursum
denticulatis, subcontinuis fuligineis; conidiis ex elliptico fusoideis,
variae longitudinis, 14 × 4.5, v. 6–9 × 3.7–4.5 μm, olivaceo-fuscis,
continuis v. rarius 1-septatis, levibus, rarius intermixtis ramoconidiis 1–2-apiculatis.
Notes: The following authentic material from PAD has been
examined: on a dead leaf of Amaranthus sp., Malta, Jan. 1914, C.
Gatto, No. 501 (PAD). This collection contains a single dead leaf of
Amaranthus and possesses another number. Thus it does not fully
agree with data from the type collection. The collection examined
contains several saprobic imperfect fungi, but a Cladosporium
agreeing with the original description could not be traced. Zhang
et al. (2003) reported the species on Amaranthus caudatus (China,
Zhejiang) and A. tricolor (China, Hubei). Type material of this
species was not found.
venturioides var. citricola Sacc., Nuovo Giorn. Bot. Ital., N.S.,
22: 72. 1915.
Type: Malta, Imtahleb, on leaves (“in foliis morientibus”) of Citrus
×aurantium (Rutaceae), C. Balzan, 1014, no. 501.
Lit.: Saccardo (1931: 796).
Original diagnosis (Saccardo 1915a): Hypostromate 40–50 μm
diam.; conidiophoris 80–90 × 4–5 μm; conidiis plerumque continuis,
brevioribus, 5–7 × 2.5 μm, olivaceis.
Notes: No type material could be found at PAD.
viride (Fresen.) Z.Y. Zhang & T. Zhang, Proceedings of
Phytopathological Symposium Organized by Phytopathology
Laboratory of Yunnan Province 2: 306. 1998.
Type: On rotten fruits of Vitis (Vitaceae).
Basionym: Penicillium viride Fresen., Beitr. Mykol. 1: 21. 1850.
≡ Hormodendrum viride (Fresen.) Sacc., Syll. fung. 4: 311. 1886.
Ill.: Fresenius (1850: tab. 3, igs 16-19), Zhang et al. (2003: 175,
ig. 122).
www.studiesinmycology.org
Original diagnosis (Fresenius 1850): Aus einer liegenden Basis
aufrechte ästige Fäden, welche an der Spitze des Hauptstammes
und der Aeste sich in dichotomen Sporenketten abgliedern. Sporen
länglich und eiförmig, meist mit zwei runden Tröpfchen gezeichnet.
Notes: Type material not preserved. Recorded from China (Jiangxi)
on Vitis balanseana.
viridiolivaceum Pidopl. & Deniak → transchelii var. viridiolivacearum.
viridiolivaceum var. semenicola Pidopl. & Deniak →
transchelii var. semenicola.
vitis-frutigeni, in herb.
Specimen: USA, North Carolina, Wayneville, on fruit of Vitis labrusca (Vitaceae), 17
Sep. 1890, G.E. Boggs (BPI 427626, M-0057767).
Notes: Fruiting superial. A true Cladosporium s. str. (C.
cladosporioides-like, but conidiophores strongly geniculatesinuous). Status remains unclear.
zeae Lobik, Trudy Severo-Kavkazsk. Inst. Zashch. Rast.
1(2): 41. 1933, nom. illeg., non C. zeae Peck, 1894.
Type: Former USSR, Caucasus, on Zea mays (Poaceae).
Notes: Type material could not be traced, probably not preserved.
zizyphi [Macrosporium] Bacc., Ann. Bot. (Rome) 14(3): 140.
1917.
Type: “Eritrea, on leaves of Ziziphus spina-christi (Rhamnaceae),
a Cheren in maggio 1914”, Baldrati n. 25 (FI, IMI 98898, ex-type
slide).
Notes: Type material was examined by F.C. Deighton, who supposed
that this species could be a Cladosporium. He compared it with
Trichosporum simplex Sacc., deposited in IMI as Cladosporium
spinae-christae Deighton, ined.
367
BenSch et al.
Heterosporium
caraganae Vasyag., Izv. Akad. Nauk Kazakh. SSR, Ser. Biol.,
1813: 101. 1957.
The epithets are alphabetically listed. The generic name, i.e.,
Heterosporium, is omitted. Heterosporium was monographed by
David (1997), i.e., this list is mainly based on his examinations.
Type: Kazakhstan, Karagandinskaya Oblast, Ostraya Mt. and
Lake Saricul, on Caragana frutex (Fabaceae), 4 Jul. 1953 and 6
Jul. 1954 (AA?).
Lit.: David (1997: 131).
Notes: Description resembling Cladosporium macrocarpum, but
generic afinity unclear.
alhagi (“alhaginis”) Koshk. & Frolov, Mikol. Koped-Daga i
Karakumov: 156. 1973, nom. inval.
Type: Turkmenistan, Kutom, on dry stems of Alhagi sp. (Fabaceae),
6 Jul. 1967 (not preserved).
Lit.: David (1997: 129).
Notes: This species was published as “ad int.” and described in
Russian.
allii var. funkiae Massa, Ann. Mycol. 10: 298. 1912.
Type: Italy, Piemont, Crescentino, on dry lower stems of Funkia
(Hosta) sp. (Asparagaceae), 9 Nov. 1903, Massa (location
unknown).
Lit.: David (1997: 129).
Notes: Based on the original description (conidia 20–25 × 7–10 μm,
1–3-septate) probably identical with Cladosporium macrocarpum.
allii var. minutum Maire, Mém. Soc. Sci. Nat. Phys. Maroc
45: 136. 1937.
Type: Morocco, Moyen Atlas, Arzou, on Polygonatum odoratum
(Asparagaceae), without date, R. Maire (MPU?).
Lit.: David (1997: 129).
Notes: This variety was compared with H. allii var. polygonati
[conidiophores 60–100 μm long, conidia 14–20(–24) × 8–11 μm].
Var. minutum possibly refers to Cladosporium macrocarpum.
allii var. polygonati Oudem., Nederl. Kruidk. Arch., Ser. III, 2:
315. 1900.
Holotype: Netherlands, Gelderland, Apeldoorn, Nunspeet, on
Polygonatum multilorum (Asparagaceae), 2 Oct. 1899, C.A.G.
Beins (L).
Lit.: David (1997: 129).
Notes: The type material contains Alternaria sp., Cladosporium
herbarum, and Curvularia sp.
allii var. sisyrinchii Speg. ex Sacc., Syll. fung. 10: 659. 1892.
Holotype: Argentina, near Wallamantu, on rotting leaves of
Sisyrinchium sp. (Iridaceae), May 1882, Spegazzini (LPS?).
Lit.: David (1997: 130).
beckii Bäumler, Verh. K. K. Zool.-Bot. Ges. Wien 43: 292.
1893.
Type: Austria, Niederösterreich, Währing, on the surface of the rind
of Cucurbita sp. (Cucurbitaceae), Feb. ?1893 (location unknown).
Lit.: David (1997: 130).
Notes: Conidiophores densely packed from a stroma; conidia
kidney-shaped (David 1997, citing sources). In David’s (1997)
opinion probably not a species of Cladosporium.
betae Dowson, Mycol. Centralbl. 2: 3. 1913.
Type: Germany, Hamburg, on leaves of Beta vulgaris
(Amaranthaceae), Oct. 1911, Klebahn (location unknown).
Lit.: David (1997: 130).
Notes: Probably belonging to the Cladosporium herbarum complex,
maybe C. macrocarpum (conidia 13–24 × 6–12 μm, 1–2-septate).
368
celastrinum Tilak, Curr. Sci. 32: 422. 1963.
Holotype: India, Maharashtra, Daulatabad, on dead stems of
Celastrus paniculatus (Celastraceae), 20 Feb. 1963, S.T. Tika
(herb. Marathwada Univ., not available).
Lit.: David (1997: 131).
Notes: Possibly identical with Dendryphiella vinosa.
centaureae Ranoj., Ann. Mycol. 8: 398. 1910.
Type: Serbia, Belgrade, Topcider, on dry stems of Centaurea
apiculata subsp. spinulosa (≡ C. spinulosa) (Asteraceae), 26 Mar.
1910, Ranojevic (location unknown).
Lit.: David (1997: 132).
Notes: Conidiophores from a stroma; conidia sometimes clavate
(David 1997, citing sources). Status unclear.
cercosporoides (“cercosporoide”) Speg., Bol. Acad. Nac. Ci.,
Córdoba 11: 305. 1888.
Holotype: Argentina, Tierra del Fuego, I. de los Estados, on dying
leaves of Agrostis magellanica (Poaceae), Feb. 1882, Spegazzini
(LPS?).
Lit.: David (1997: 132).
Notes: Probably not belonging to Cladosporium.
elmeri Syd. & P. Syd., Leal. Philipp. Bot. 9: 3132. 1925.
Syntype: Philippines, Luzon, Sorsogon Prov., Irosin (Mt. Bulusan),
on Ochrosia apoensis (Apocynaceae), Apr. 1915 [? 1916], Elmer
14968 & 14969 (location unknown).
Notes: David (1997) examined syntype material from K
and NY (Elmer 14969), which was, however, devoid of any
fungal fructiication agreeing with the original description (in
hypophyllous cushions, conidiophores short, 15–38 μm, conidia
at irst 0–1-septate, 20 μm long, later 2–5-septate, up to 50 × 5–7
μm).
eremostachydis Golovin, Trudy Sredneaziats. Gosud. Univ.,
N.S., 14(5): 25. 1950.
Type: Tadzhikistan, Pamir, Dschauschongoz, on stems of
Eremostachys sp. (Lamiaceae), 10 Aug. 1935, ?Golovin (location
unknown).
Lit.: David (1997: 132).
Notes: Status unclear, probably no a member of Cladosporium.
eucalypti Ellis & Everh., Proc. Acad. Nat. Sci. Philadelphia
1894: 381. 1894.
Holotype: USA, California, on Eucalyptus sp. (Myrtaceae), 28 Nov.
1893, A.J. McClatchie 542 (NY).
Lit.: David (1997: 132).
Notes: Based on the original description, this species could be
identical with Cladosporium macrocarpum, but David’s examination
showed rather “Fumago-like” crusts with smaller conidia.
the genuS Cladosporium
gracile var. muscari (“muscaridis”) Pat., Énum. champ.
Tunisie: 18. 1892.
Type: Tunisia, Kroussiah, without locality and date, on leaves of
Muscari comosum (Asparagaceae) (location unknown).
Lit.: David (1997: 133).
Ill.: Patouillard (1892: pl. II, ig. 10).
Notes: According to David (1997), this variety is probably a true,
Heterosporium-like Cladosporium species, but rather similar to
C. ornithogali. However, type material could not be traced. This
variety was not listed in Pister (1977). Koshkelova (1977) recorded
it from southern Turkmenistan on Gladiolus italicus (= G. segetum)
(Iridaceae) and Muscari leucostomum.
graminis McAlpine, Proc. Linn. Soc. New South Wales 1904:
122. 1904.
Holotype: Australia, Victoria, Melbourne, St. Kilda, on Ammophila
arenaria (Poaceae), 14 Sep. 1903, D. McAlpine (VPRI 2673).
Lit.: David (1997: 133).
Notes: The type material consists of a mixture of a Stemphylium
and a true Cladosporium with catenate conidia, ca. 18–30 × 9–12(–
14) μm.
graminum Rostr., Handb. Læren Plantesygd. Landbr. Havebr.
Skovbr.: 607. 1902.
Type: On Dactylis glomerata and Triticum aestivum (Poaceae),
details unknown (location unknown, neither at C nor CP).
Lit.: David (1997: 134).
Notes: Type material could not be traced, and the original
description is very brief and barely diagnostic (conidiophores
knotted, brown, conidia long, brown, echinulate, 3–4-septate). Lind
(1913) suggested that this species was identical to Cladosporium
graminis (= C. herbarum), which is very probable.
groenlandicum Allesch., Bibl. Bot. 42: 54. 1997.
Type: Greenland, Umanak, on dead leaves of Papaver nudicaule
(Papaveraceae), 18 Jun. 1892, Allescher (location unknown, not
at M).
Lit.: David (1997: 134).
Notes: Allescher’s type material could not be located. David (1997)
examined material deposited under this name by Potebnia (PAD),
which could be identiied as Cladosporium macrocarpum.
lallemantiae (“lallemantii”) Khokhr., Bolez. Vredit. Maslichnykh
Kul’tur 1(2): 33. 1934.
Holotype: Russia, Gor’kyi Oblast, Novgorod, near Vjatka,
Agrobiological Station nurseries, on Lallemantia sp. (Lamiaceae),
17 Aug. 1931, F. Fokin (LEP).
Lit.: David (1997: 134).
Notes: David (1997) examined type material and found a mixture
of several fungi, including Alternaria alternata, Cladosporium
herbarum and Oidium sp. The original description was probably
based on Alternaria conidiophores and Cladosporium conidia.
laricis Cooke & Massee, Grevillea 16: 80. 1888.
Type: UK, Norfolk, without detailed location, on leaves of Larix
decidua (Pinaceae), without date and collector (location unknown,
not at K).
Lit.: David (1997: 135).
Notes: Identity unclear, but in any case not belonging to
Cladosporium s. str.
www.studiesinmycology.org
lobeliae Speg., Revista Fac. Agron. Univ. Nac. La Plata, Ser.
2, 6: 188. 1910.
Holotype: Chile, Cerro Alegre de Valparaiso, on Lobelia excelsa (=
L. salicifolia) (Campanulaceae), [1908], Spegazzini (LPS?).
Lit.: David (1997: 135).
Notes: According to David (1997), synonymous with Cladosporium
macrocarpum.
lonicerae Negru, Trudy Bot. Inst. Akad. Nauk SSSR, Ser. 2,
Sporov. Rast. 12: 223. 1959.
Type: Romania, Cluj, on living and dead stems of Lonicera tatarica
and L. sullivantii (Caprifoliaceae), 22 May 1956, ?Negru (location
unknown).
Lit.: David (1997: 135).
Notes: According to David (1997), very probably identical with
Cladosporium macrocarpum.
luci Chevaug., Encycl. Mycol. 28: 94. 1956.
Type: Senegal (Sefa), Ivory Coast (Adiopodoumé) and tropical
Africa (without any locality), on stems and young branches of
Manihot esculenta (= M. utilissimus) (Euphorbiaceae), Crotalaria
retusa (Fabaceae) and Citrus sp. (Rutaceae) (location unknown,
not at PC, possibly at ABI, but not seen).
Lit.: David (1997).
Notes: According to David (1997), probably not belonging to
Cladosporium s. str., but rather cercosporoid (Passalora, incl.
Phaeoramularia).
medicaginis Karimov, Trudy Bot. Inst. Akad. Nauk SSSR,
Ser. 2, Sporov. Rast. 11: 129. 1956.
Type: Uzbekistan, Tashkent, on leaves of Medicago sativa
(Fabaceae), 19 Aug. 1952, Karimov (location unknown).
Lit.: David (1997).
Notes: Based on the original description and illustration, this
species is probably a synonym of Cladosporium macrocarpum. It
was also reported from Turkmenistan by Koshkelova (1977).
onobrychidis Chevassut, Bull. Féd. Mycol. Dauphiné-Savoie
27: 9. 1987, nom. inval.
Type: France, Dauphiné-Savoie, Doucy, on Onobrychis viciifolia
(Fabaceae), 11 Jun. 1986, H. Robert (location unknown).
Lit.: David (1997: 136).
Notes: Based on the original description, this species is probably a
synonym of Cladosporium herbarum.
phragmitis Sacc., Rev. Mycol. (Toulouse) 6: 37. 1884 [as
“(Opiz) Sacc.”].
Holotype: Belgium, Ardennes, on Phragmites australis (Poaceae),
without date, Libert, Reliqu. libertianae 382 (PAD).
= Cladosporium phragmitis J. Opiz, Seznam: 117. 1852, nom. inval., non
C. phragmitis J. Opiz ex Oudem., 1892.
Lit.: David (1997: 137).
Notes: The complicated nomenclature and history of the confusion
of the names involved were discussed by David (1997), who
found in the type collection a typical “Fumago”, i.e., a mixture of
Aureobasidium pullulans and Cladosporium.
phragmitis var. inlorescentiae Bubák, Ann. Mycol. 13: 34.
1915.
Holotype: Czech Republic, Mähr. [Hranice] Weisskirchen, Becawa
[Becva] River, [illegible], on Phalaris arundinacea (Poaceae), Sep.
369
BenSch et al.
1911, F. Petrak (BPI 802191).
Lit.: David (1997: 137).
Notes: According to David (1997), a typical “Fumago”, i.e., a mixture
of Aureobasidium pullulans and Cladosporium.
Notes: The type material proved to be a member of the Cladosporium
herbarum complex, either C. herbarum s. str. or C. macrocarpum
(conidiophores nodulose, conidia verruculose, 11.5–25 × 6.5–9
μm).
polymorphum Nicolas & Aggéry, Bull. Soc. Hist. Nat. Toulouse
58: 139. 1939.
tupae Speg., Revista Fac. Agron. Univ. Nac. La Plata, Ser. 2,
6: 188. 1910.
Type: France, Toulouse, Botanic Garden and garden of the
University of Toulouse, on leaves of Viburnum odoratissimum
(Adoxaceae), without date and collector (location unknown, TL?).
Lit.: David (1997: 138).
Notes: Based on the original description, possibly Cladosporium
herbarum.
Holotype: Chile, Valdivia, on leaves of Lobelia bridgesii
(Campanulaceae), without date [1908], Spegazzini (LPS?).
Lit.: David (1997: 139).
Notes: Not examined, status unknown.
savulescui E. Radul. & Negru, in Bontea et al. (Eds.), Omagiu
lui Traian Sâvulescu: 651. 1959.
Type: Romania, Cluj, Botanic Garden, on leaves of Thalictrum
minus (Ranunculaceae), 10 Sep. 1956, without collector (location
unknown).
Lit.: David (1997: 138).
Notes: Each conidiophore, consisting of a short side branch of
a hypha, produces a single apical conidium (David 1997, citing
sources). Not Cladosporium s. str., but status unclear.
stenhammariae Rostr., Tillæg til ‘Groenlands Svampe 1888’:
630. 1891.
Holotype: Greenland, Kaersut, on leaves of Mertensia maritima
(Boraginaceae), 4 Aug. 1888, S. Hansen (C).
Lit.: David (1997: 138).
Notes: The type material was examined by J. David, who found the
specimen to be depauperate, severely damaged by insects. Based
on the original description, this species was probably identical with
Cladosporium herbarum or C. macrocarpum.
symphoricarpi Ranoj., Ann. Mycol. 3: 398. 1910.
Type: Serbia, Belgrade, on twigs of Symphoricarpos racemosus
(Caprifoliaceae), 3 Jan. 1910, N. Ranojevic (BPI 1108751).
Lit.: David (1997: 138).
370
umbelliferarum Jacz., Opred. grib. 2: 293. 1917.
Lectotype (designated by David 1997): Russia, St. Petersburg,
Hortus Botanici Petropolitani, on stems of an unknown umbellifer
(Apiaceae), 1883, Korjinski (LEP).
Lit.: David (1997: 139).
Notes: J. David examined type material of this fungus and found
a mixture of an Alternaria and a member of the Cladosporium
herbarum complex (very probably C. herbarum s. str.).
vellosoanum Speg., Anales Mus. Nac. Hist. Nat. Buenos
Aires, Ser. 3, 20: 442. 1910.
Holotype: Argentina, Jujuy, Orán, on leaves of Mimosa velloziana
(Fabaceae), Mar. 1905, ?Spegazzini (LPS?).
Lit.: David (1997: 139).
Notes: According to the original description, this species is
“extremely unlikely” to be a member of the genus Cladosporium s.
str. (David 1997).
xanthosomatis (“xanthosomae”) Gonz. Frag. & Cif., Publ.
Estac. Agron. Moca, Ser. B, Bot., 11: 53. 1928, nom. inval.
Lit.: David (1997: 140).
Notes: This name was invalidly published, introduced as “ad int.”. It
is probable that this name was the remnant of an earlier description
of Helminthosporium xanthosomatis, which was introduced in the
same paper. The collection details agree to those for Cercospora
xanthosomatis.
the genuS Cladosporium
ACKNOWLEDGEMENTS
The cooperation of the following institutions in locating type and authentic material
is gratefully acknowledged: B, BPI, BRIP, C, CUP, DAOM, DAR, FH, HAL, HBG,
IACM, ILL, IMI, INIFAT, K, KR, LBLM, LE, LEP, LPS, M, MA, NY, NYS, PAD, PC,
PDD, PH, PPMH, PRM, S, SIENA, VPRI, W, WIS. We are very grateful to the
directors and curators for loaning type material and other collections in their keeping
during the course of monographic studies of the genus Cladosporium. We like to
thank J.H. Cunnington (Australia), J. David (UK), Y.L. Guo (China), G.S. de Hoog
(the Netherlands), J. Mouchacca (France), D. Seidel (Germany) and Z.Y. Zhang
(China) for their support in the search for rare publications. We are much obliged to
V.A. Mel'nik (Russia) for his continuous support in locating type material and other
collections in Russian herbaria, for providing and translating rare publications in
Cyrillic and critical notes on the manuscript. The technical staff of the CBS, Marjan
Vermaas (photo plates), Arien van Iperen (cultures), and Trix Merkx (deposit of
strains) are thanked for their invaluable assistance. The reviewers are gratefully
acknowledged for their useful suggestions and corrections. We are indebted
to C. Nakashima (Japan) who translated the description of Hormodendrum mori
from Japanese. Several colleagues from different countries provided material and
valuable cultures over the past few years without which this work would not have
been possible. In this regard, we are grateful to M. Arzanlou (Iran), W. von Brackel
(Germany), F.M. Dugan (USA), F. Freire (Brazil), W. Gams (Netherlands), D. Glawe
(USA), A.G. Greslebin (Argentina), B. Heuchert (Germany), F. Hill († New Zealand),
R. Kirschner (Germany), W. Mułenko (Poland), A. Ramaley (USA), K.A. Seifert
(Canada), H.-D. Shin (South Korea) and L.J. du Toit (USA).
www.studiesinmycology.org
371
BenSch et al.
REFERENCES
Aarnaes JO (2000). Catalogue over macro- and micromycetes recorded from
Norway and Svalbard. Synopsis fungorum 16: I-viii; 1–412.
Ahmad S (1969). Fungi of West Pakistan. Supplement I. Biological Society of
Pakistan Monograph 5: 1–110.
Alieri JSA, Langdon KR, Wehlburg C, Kimbrough JW (1984). Index of Plant
Diseases in Florida (Revised). Florida Department of Agriculture and Consumer
Service, Bulletin of the Division of Plant Industries 11: 1–389.
Anilkumar TB, Seshadri VS (1975). Cladosporium leaf spot of sunlower. Current
Science 44(19): 722.
Aptroot A (2006). Mycosphaerella and its anamorphs: 2. Conspectus of
Mycosphaerella. CBS Biodiversity Series 5: 1–231.
Arens K (1945). Um fungo destruidor de pinturas a oleo: Cladosporium herbarum
(Pers.) var. nigricans (Roth). Summa Brasiliensis Biologiae 1: 1–13.
Arx JA von (1950). Über die Ascusform von Cladosporium herbarum (Pers.) Link.
Sydowia 4: 320–324.
Arx JA von (1981). The genera of fungi sporulating in pure culture. 3rd ed. Cramer,
Vaduz.
Arx JA von (1983). Mycosphaerella and its anamorphs. Proceedings, Koninklijke
Nederlandse Akademie van Wetenschappen C, 86(1): 15–54.
Arx JA von (1987). Plant pathogenic fungi. Beihefte zur Nova Hedwigia 87: 1–288.
Arya C, Arya A (2003). New leaf spot diseases of social forestry trees – II. Journal of
Mycology and Plant Pathology 33(2): 320–322.
Arzanlou M, Groenewald JZ, Gams W, Braun U, Shin H-D, Crous PW (2007).
Phylogenetic and morphotaxonomic revision of Ramichloridium and allied
genera. Studies in Mycology 58: 57–93.
Arzanlou M, Groenewald JZ, Fullerton RA, Abeln ECA, Carlier J, Zapater M-F,
Buddenhagen IW, Viljoen A, Crous PW (2008). Multiple gene genealogies and
phenotypic characters differentiate several novel species of Mycosphaerella
and related anamorphs on banana. Persoonia 20: 19–37.
Assante G, Mafi D, Saracci M, Farina G, Moricca S, Ragazzi A (2004).
Histological studies on the mycoparasitism of Cladosporium tenuissimum on
urediniospores of Uromyces appendiculatus. Mycological Research 108 (2):
170–182.
Bagyanarayana G, Braun U (1999). Phytopathogenic micromycetes from India (II).
Sydowia 1(1): 1–19.
Baka ZAM, Krzywinski K (1996). Fungi associated with leaf spots of Dracaena
ombet (Kotschy and Peyr). Microbiological Research 151: 49–56.
Baker KF, Dimock AW, Davis LH (1950). Ramularia cyclaminicola Trel., the cause of
Cyclamen stunt disease. Phytopathology 40: 1027–1034.
Baldacci E, Ciferri R (1937). Un nuovo genere di micete parassita del pioppo
Pollacia radiosa (Lib.) Baldacci e Ciferri, Revisione dei G. Stigmella e Stigmina.
I. Pollaccia radiosa (Lib.) Baldacci e Ciferri. Atti dell’Istituto Botanico “Giovanni
Briosi” et Laboratorio Crittogamica Italiano della Reale Università di Pavia, Ser.
4, 10: 55–72.
Barr ME (1958). Life history studies of Mycosphaerella tassiana and M. typhae.
Mycologia 50: 501–513.
Barron GL (1968). The genera of hyphomycetes from soil. Williams & Wilkins Co.,
Baltimore.
Bayliss Elliott JS (1914). Fungi in the nests of ants. Transactions of the British
Mycological Society 5(1): 138–142.
Beck A, Ritschel A, Schubert K, Braun U, Triebel D (2005). Phylogenetic relationships
of the anamorphic genus Fusicladium s. lat. as inferred by ITS nrDNA data.
Mycological Progress 4(2): 111–116.
Beeli M (1923). Notes mycologiques. Champignons nouveaux pour la lore Belge,
récoltés de 1915 a 1923. Bulletin de la Société Royale de Botanique de
Belgique 56: 68.
Benjamin CR, Slot A (1969). Fungi of Haiti. Sydowia 23: 125–163.
Bensch K, Groenewald JZ, Dijksterhuis J, Starink-Willemse M, Andersen B,
Shin H-D, Dugan FM, Schroers H-J, Braun U, Crous PW (2010). Species
and ecological diversity within the Cladosporium cladosporioides complex
(Davidiellaceae, Capnodiales). Studies in Mycology 67: 1–94.
Berkeley MA (1843). Notices of some Brazilian fungi. London Journal of Botany 2:
629–643.
Berkeley MJ (1869). On a collection of fungi from Cuba. Part II., including those
belonging to the families Gasteromycetes, Coniomycetes, Hyphomycetes,
Physomycetes, and Ascomycetes. Journal of the Linnean Society, Botany 10:
341–392.
Berkeley MJ (1875). Notices of North American fungi. Grevillea 3(27): 97–112.
Berkeley MJ, Curtis MA (1860). Characters of new fungi, collected in the North
Paciic Exploring Expedition by Charles Wright. Proceedings of the American
Academy of Arts and Sciences 4: 111–130.
Berlese AN (1895). Prima contribuzione allo studio della morfologia e biologia di
Cladosporium e Dematium. Rivista di Patologia Vegetale 4: 1–45.
Berlese AN, Voglino P (1886). Sylloge fungorum. Additamenta ad Volumina I.–IV.
Padova.
372
Bilgrami KS, Jamaluddin S, Rizwi MA (1991). Fungi of India. List and references
(second revised and enlarged and brought up to date edition). New Delhi.
Bontea V (1985). Ciuperci parazite şi saproite din România. Vol. I. Editura
Academici Republicii Socialiste România, Bucureşti.
Bontea V (1986). Ciuperci parazite şi saproite din România. Vol. II. Editura
Academici Republicii Socialiste România, Bucureşti.
Bontea V, Dumitraş L (1967) Two new dematiaceae. Revue Roumaine de Biologie,
Série de Botanique 12(6): 387–390.
Brackel W von (2007). Weitere Funde lechtenbewohnender Pilze in Bayern. Beitrag
zu einer Checkliste III. Berichte der Bayerischen Botanischen Gesellschaft 77:
5–26.
Brackel W von (2008). Some lichenicolous fungi collected during the 20th meeting
of the Società Lichenologica Italiana in Sienna. Notiziario del Società
Lichenologica Italiana 21: 63–66.
Brackel W von (2009). Weitere Funde von lechtenbewohnenden Pilzen in Bayern
– Beitrag zu einer Checkliste IV. Berichte der Bayerischen Botanischen
Gesellschaft 79: 5–55.
Brandenburger W (1985). Parasitische Pilze an Gefäßplanzen in Europa. Fisher
Verlag, Stuttgart, New York.
Braun A (1853) Über einige neue oder weniger bekannte Planzenkrankheiten,
welche durch Pilze erzeugt werden. Verhandlungen des Vereins zur
Beförderung des Gartenbaues in den Königlich Preussischen Staaten 1:
165–199.
Braun U (1994). Studies on Ramularia and allied genera (VII). Nova Hedwigia
58(1–2): 191–222.
Braun U (1995a). Miscellaneous notes on phytopathogenic hyphomycetes (II).
Mycotaxon 55: 223–241.
Braun U (1995b). A monograph of Cercosporella, Ramularia and allied genera
(phytopathogenic hyphomycetes) Vol. 1. IHW-Verlag, Eching.
Braun U (1998). A monograph of Cercosporella, Ramularia and allied genera
(phytopathogenic hyphomycetes) Vol. 2. IHW-Verlag, Eching.
Braun U (2000). Miscellaneous notes on some micromycetes. Schlechtendalia 5:
31–56.
Braun U (2001). Cladosporium exoasci, C. exobasidii and some allied species.
Schlechtendalia 7: 53–58.
Braun U (2002). Miscellaneous notes on some micromycetes (II). Schlechtendalia
8: 33–38.
Braun U (2003). Miscellaneous notes on some cercosporoid hyphomycetes.
Bibliotheca Lichenologica 86: 79–98.
Braun U (2007). Fungi selecti exsiccati ex Herbario Universitatis Halensis – nos.
71–110. Schlechtendalia 16: 77–91.
Braun U (2009). New species, combinations and records of hyphomycetes.
Schlechtendalia 19: 63–71.
Braun U, Cook RTA, Inman AJ, Shin H-D (2002). The taxonomy of the powdery
mildew fungi. In: The powdery mildews, a comprehensive treatise (Bélanger
RR et al., eds). APS Press, St. Paul, USA: 13–55.
Braun U, Crous PW (2011). Keys. Cladosporium and similar genera. pp. 897–901.
In: The Genera of Hyphomycetes (Seifert K, Morgan-Jones G, Gams W,
Kendrick B, eds.) CBS Biodiversity Series 9: 1–997.
Braun U, Crous PW, Dugan FM, Groenewald JZ, Hoog GS de (2003). Phylogeny
and taxonomy of cladosporium-like hyphomycetes, including Davidiella
gen. nov., the teleomorph of Cladosporium s.str. Mycological Progress
2(1): 3–18.
Braun U, Crous PW, Schubert K (2008a). Taxonomic revision of the genus
Cladosporium s. lat. 8. Reintroduction of Graphiopsis (= Dichocladosporium)
with further reassessments of cladosporioid hyphomycetes. Mycotaxon 103:
207–216.
Braun U, Crous PW, Schubert K, Shin H-D (2010). Some reallocations of Stenella
species to Zasmidium. Schlechtendalia 20: 99–104.
Braun U, Cunnington J, Priest MJ, Shivas RG, Schubert K (2005). Annotated checklist of Ramularia species in Australia. Australasian Plant Pathology 34: 1–7.
Braun, U, Freire F (2004). Some cercosporoid hyphomycetes from Brazil – III.
Cryptogamie, Mycologie 25(3): 221–244.
Braun U, Hill CF, Schubert K (2006). New species and new records of biotrophic
micromycetes from Australia, Fiji, New Zealand and Thailand. Fungal Diversity
22: 13–35.
Braun U, Mel’nik VA (1997). Cercosporoid fungi from Russia and adjacent countries.
Trudy Botanicheskogo Instituta im V.L. Komarova (St. Petersburg) 20: 1–130.
Braun U, Mel’nik VA, Schubert K (2008b). Two new species of the hyphomycete
genus Cladosporium. Mikologia i Fitopatologia 42(3): 214–220.
Braun U, Mouchacca J, McKenzie EHC (1999). Cercosporoid hyphomycetes from
New Caledonia and some other South Paciic islands. New Zealand Journal of
Botany 37: 297–327.
Braun U, Rogerson CT (1995). Parasitic hyphomycetes from Utah (USA) - II.
Sydowia 47(2): 141–145.
Braun U, Schubert K (2002). Fusicladium scillae. IMI Descriptions of Fungi and
Bacteria 152, No. 1518.
the genuS Cladosporium
Braun U, Schubert K (2007). Taxonomic revision of the genus Cladosporium s.
lat. 7. Descriptions of new species, a new combination and further new data.
Schlechtendalia 16: 61−76.
Bridson GDR, Smith ER (1991). Botanico-Periodicum-Huntianum/Supplementum.
Carnegie Mellon University. Pittsburgh, PA. Allen Press Inc., KS.
Brondeau ML de (1854). Description du Cladosporium dufourii. Archives de Flore,
Journal Botanique 1: 59–60.
Brown KB, Hyde KD, Guest DI (1998). Preliminary studies on endophytic fungal
communities of Musa acuminata species complex in Hong Kong and Australia.
Fungal Diversity 1: 27–51.
Brummitt RK, Powell CE (1992). Authors of Plant Names. Royal Botanic Gardens,
Kew.
Brunaud MP (1889). Champignons a ajouter a la lore mycologique des environs
de Saintes (3e série). Bulletin de la Société Botanique de France 36: 335–340.
Bubák F (1916). Einige neue oder kritische Pilze aus Kanada. Hedwigia 58: 15–34.
Bugnicourt F (1958). Contribution à l’étude de Cladosporium colocasiae Sawada.
Revue de Mycologie 23: 233–236.
Burgess TI, Barber PA, Sufaati S, Xu D, Hardy GEStJ, Dell B (2007). Mycosphaerella
spp. on Eucalyptus in Asia: New species, new host and new records. Fungal
Diversity 24: 135–157.
Burri R, Staub W (1909). Monilia nigra als Ursache eines Falles von
Schwarzleckigkeit bei Emmentalerkäse. Landwirtschaftliches Jahrbuch der
Schweiz 23: 479–513?.
Buzina W, Braun H, Freudenschuss K, Lackner A, Stammberger H (2003). Fungal
biodiversity as found in nasal mucus. Medical Mycology 41: 149–161.
Carbone D (1914). Diagnosi latina delle specie nuove. Atti dell’Istituto Botanico e del
Laboratorio Crittogamico dell’Universita di Pavia 14: 320–323.
Carbone I, Kohn LM (1999). A method for designing primer sets for speciation
studies in ilamentous ascomycetes. Mycologia 91: 553–556.
Carrión A, Silva M (1955). Sporotrichosis special reference: A revision of socalled Sporotrichum gougerotii. American Medical Association Archieves of
Dermatology 72: 523–534.
Cash EK (1952). A record of the fungi named by J.B. Ellis., Part I. Divison of
Mycology and Disease Survey, Bureau of Plant Industry, Soils, and Agricultural
Engineering, Agricultural Research Administration, USDA.
Castañeda RF (1987). Fungi cubensis II. Instituto de Investigaciones Fundamentales
en Agricultura Tropical “Alejandro de Humbolt”, Havana, Cuba.
Castañeda RF, Kendrick B (1991). Ninety-nine conidial fungi from Cuba and three
from Canada. University of Waterloo Biology Series 35: 1–132.
Castañeda RF, Kendrick B, Gené J (1997). Notes on conidial fungi. XIII. A new
species of Cladosporium from Cuba. Mycotaxon 63: 183–187.
Cattaneo A (1879). Contributo allo studio die miceti che mascono sulle pianticelle di
Riso. Archivio Triennale del Laboratorio di Botanica Crittogamica 2–3: 115–128.
Cheewangkoon R, Crous PW, Hyde KD, Groenewald JZ, To-anan C (2008). Species
of Mycosphaerella and related anamorphs on Eucalyptus leaves from Thailand.
Persoonia 21: 77–91.
Chupp C (1954) A monograph of the fungus genus Cercospora. Ithaca, New York.
Published by the author.
Ciferri R (1960). Manuale de Micologica Medica, Tomo II, 2nd ed. Casa Editrice Reno
Cortina, Pavia.
Ciferri R (1962). Schedae mycologicae. XXXV–XCVIII. Atti dell’Istituto Botanico
della Università e Laboratorio Crittogamico di Pavia, Ser. 5, 19(5): 85–139.
Clements FE, Shear CL (1931). The Genera of Fungi. H.W. Wilson, New York.
Cook RTA, Inman AJ, Billings C (1997). Identiication and classiication of powdery
mildew anamorphs using light and scanning electron microscopy and host
range data. Mycological Research 101(8): 975–1002.
Cooke MC (1871). Handbook of British fungi, Vol. II. MacMillan and Co., London.
Cooke MC (1876). Some Indian fungi. Grevillea 5(33): 14–17.
Cooke MC (1879). New Zealand fungi. Grevillea 8(46): 54–68.
Cooke MC (1883). New American Fungi. Grevillea 12(61): 22–33.
Cooke MC (1888). New British Fungi. Grevillea 16(79): 77–81.
Cooke MC (1889). Omitted diagnoses. Grevillea 17(83): 65–69.
Cooke MC, Ellis JB (1877). New Jersey fungi. Grevillea 6(37): 1–18.
Cooke WB (1950). Western fungi – I. Mycologia 41: 601–622.
Cooke WB (1962). A taxonomic study in the “black yeasts”. Mycopathologia et
Mycologia Applicata 17: 1–43.
Corda ACJ (1831). Deutschlands Flora, Abtheilung III. Die Pilze Deutschlands, Band
3, Heft 12. Nürnberg.
Corda ACJ (1833). Deutschlands Flora, Abtheilung III. Die Pilze Deutschlands, Band
3, Heft 13. Nürnberg.
Corda ACJ (1837). Icones fungorum hucusque cognitorum. Vol. 1. Praha.
Corda ACJ (1839). Icones fungorum hucusque cognitorum. Vol. 3. Praha.
Crane JL, Shearer CA (1991). A nomenclatur of Leptosphaeria V. Cesati & G. de
Notaris (Mycota-Ascomycotina-Loculoascomycetes). Illinois Nature History
Survey 34(3): 195–355.
Crous PW (1998). Mycosphaerella spp. and their anamorphs associated with leaf
spot diseases of Eucalyptus. Mycologia Memoir 21: 1–170.
www.studiesinmycology.org
Crous PW, Braun U (1996). Cercosporoid fungi from South Africa. Mycotaxon 57:
233–321.
Crous PW, Braun U (2003). Mycosphaerella and its anamorphs: 1. Names published
in Cercospora and Passalora. CBS Biodiversity Series 1: 1–571.
Crous PW, Braun U, Groenewald JZ (2007a). Mycosphaerella is polyphyletic.
Studies in Mycology 58: 1–32.
Crous PW, Braun U, Schubert K, Groenewald JZ [Eds.] (2007b). The genus
Cladosporium and similar dematiaceous hyphomycetes. Studies in Mycology
58: 1–253.
Crous PW, Braun U, Schubert K, Groenewald JZ (2007c). Delimiting Cladosporium
from morphologically similar genera. Studies in Mycology 58: 33–56.
Crous PW, Braun U, Wingield MJ, Wood AR, Shin H-D, Summerell BA, Alfenas AC,
Cumagun CJR, Groenewald JZ (2009a). Phylogeny and taxonomy of obscure
genera of microfungi. Persoonia 22: 139–161.
Crous PW, Câmara MPS (1998). Cercosporoid fungi from Brazil 2. Mycotaxon 68:
299–310.
Crous PW, Gams W, Stalpers JA, Robert V, Stegehuis G (2004a). MycoBank: an
online initiative to launch mycology into the 21st century. Studies in Mycology
50: 19–22.
Crous PW, Groenewald JZ (2005). Hosts, species and genotypes: opinions versus
data. Australasian Plant Pathology 34: 463–470.
Crous PW, Groenewald JZ (2011). Why everlastings don’t last. Persoonia 26: 70–84.
Crous PW, Groenewald JZ, Mansilla JP, Hunter GC, Wingield MJ (2004b).
Phylogenetic reassessment of Mycosphaerella spp. and their anamorphs
occurring on Eucalyptus. Studies in Mycology 50: 195–214.
Crous PW, Kang JC, Braun U (2001). A phylogenetic redeinition of anamorph
genera in Mycosphaerella based on ITS rDNA sequences and morphology.
Mycologia 93: 1081–1101.
Crous PW, Phillips AJL, Baxter AP (2000). Phytopathogenic fungi from South Africa.
Department of Plant Pathology, University of Stellenbosch.
Crous PW, Schoch CL, Hyde KD, Wood AR, Gueidan C, Hoog GS de, Groenewald
JZ (2009b). Phylogenetic lineages in the Capnodiales. Studies in Mycology
64: 17–47.
Crous PW, Schroers H-J, Groenewald JZ, Braun U, Schubert K (2006).
Metulocladosporiella gen. nov. for the causal organism of Cladosporium
speckle disease of banana. Mycological Research 110: 264–275.
Crous PW, Schubert K, Braun U, Hoog GS de, Hocking AD, Shin H-D, Groenewald JZ
(2007d). Opportunistic, human-pathogenic species in the Herpotrichiellaceae
are phenotypically similar to saprobic or phytopathogenic species in the
Venturiaceae. Studies in Mycology 58: 185–217.
Crous PW, Summerell BA, Carnegie AJ, Wingield MJ, Groenewald JZ (2009c).
Novel species of Mycosphaerellaceae and Teratosphaeriaceae. Persoonia 23:
119–146.
Crous PW, Summerell BA, Mostert L, Groenewald JZ (2008a). Host speciicity and
speciation of Mycosphaerella and Teratosphaeria species associated with leaf
spots of Proteaceae. Persoonia 20: 59–86.
Crous PW, Summerell BA, Swart L, Denman S, Taylor JE, et al. (2011a). Fungal
pathogens of Proteaceae. Persoonia 27: 20–45.
Crous PW, Tanaka K, Summerell BA, Groenewald JZ (2011b). Additions to the
Mycosphaerella complex. IMA Fungus 2(1): 49–64.
Crous PW, Verkley GJM, Groenewald JZ, Samson RA (eds) (2009d). Fungal
Biodiversity. CBS Laboratory Manual Series 1: 1–269. Centraalbureau voor
Schimmelcultures, Utrecht, Netherlands.
Crous PW, Wingield MJ, Groenewald JZ (2009e). Niche sharing relects a poorly
understood biodiversity phenomenon. Persoonia 22: 83–94.
Crous PW, Wood AR, Okada G, Groenewald JZ (2008b). Foliicolous microfungi
occurring on Encephalartos. Persoonia 21: 135–146.
Cugini G, Macchiati L (1891). Notizie intorno agli insetti, acari e parasiti vegetali
nelle piante del Modenese nell’anno 1890. Bollettino Reale Stazione Agraria
di Modena, N.S. 10: 1–19.
Dangeard PCA (1931). Application de cette terminologie à l’étude des champignons,
deuxième partie. Le Cladosporium versicolor. Le Botaniste 22: 455–469.
Das AK (2003). Two new species of Cladosporium. Indian Phytopathology 56(2):
164–167.
David JC (1988a). Cladosporium colocasiae. Mycopathologia 103: 115–116.
David JC (1988b). Cladosporium echinulatum. Mycopathologia 103: 117–118.
David JC (1988c). Cladosporium musae. Mycopathologia 103: 119–120.
David JC (1988d). Cladosporium pipericola. Mycopathologia 103: 123–124.
David JC (1988e). Cladosporium phlei. Mycopathologia 103: 121–122.
David JC (1995a). Cladosporium magnusianum. Mycopathologia 129: 53–54.
David JC (1995b). Cladosporium ornithogali. Mycopathologia 129: 55–56.
David JC (1995c). Cladosporium variabile. Mycopathologia 129: 57–58.
David JC (1997). A contribution to the systematics of Cladosporium. Revision of the
fungi previously referred to Heterosporium. Mycological Papers 172: 1–157.
David JC, Kelley J (1995). Amorphotheca resinae. Mycopathologia 129: 59–62.
De TK, Chattopadhyay BK (1994). Studies in some foliicolous hyphomycetes fungi
from India. Journal of Economic and Taxonomic Botany 18(1): 225–229.
373
BenSch et al.
Deighton FC (1967). Studies on Cercospora and allied genera. II. Passalora,
Cercosporidium and some species of Fusicladium on Euphorbia. Mycological
Papers 112: 1–80.
Deighton FC (1969). Microfungi. IV: Some hyperparasitic hyphomycetes, and a note
on Cercosporella uredinophila Sacc. Mycological Papers 118: 1–41.
Deighton FC (1974). Studies on Cercospora and allied genera. V. Mycovellosiella
Rangel, and a new species of Ramulariopsis. Mycological Papers 137: 1–75.
Deighton FC (1976). Studies on Cercospora and allied genera. VI. Pseudocercospora
Speg., Pantospora Cif., and Cercoseptoria Petr. Mycological Papers 140:
1–168.
Deighton FC (1979). Studies on Cercospora and allied genera. VII. New species
and redispositions. Mycological Papers 144: 1–56.
Deighton FC (1986). Misidentiication of Cercospora effusa. Transactions of the
British Mycological Society 86: 637–641.
Desmazières MJBHJ (1851). Dixneuvième notice sur les plantes cryptogames,
récemment découvertes en France. Annales des Sciences Naturelles
Botanique, 3. Sér., 16: 296–330.
Dodge CW (1935). Medical mycology. Fungous diseases of men and other
mammals. The C.V. Mosby company. St. Louis.
Doidge EM, Bottomley AM, Plank JE van der, Pauer GD (1953). A revised list of
plant diseases in South Africa. Union of South Africa, Department of Agriculture,
Science Bulletin No. 346: 1–122.
Domsch KH, Gams W, Anderson TH (1980). Compendium of soil fungi. Vols 1 & 2.
Academic Press, London.
Drummond AJ, Ashton B, Buxton S, Cheung M, Cooper A, et al. (2011). Geneious v.
5.4, Available from http://www.geneious.com/.
Dugan FM, Braun U, Groenewald JZ, Crous PW (2008). Morphological plasticity in
Cladosporium sphaerospermum. Persoonia 21: 9–16.
Dugan FM, Glawe DA (2006). Phyllactinia guttata is a host for Cladosporium
uredinicola in Washington State. Paciic Northwest Fungi 1(1): 1–5.
Dugan FM, Rector BG (2007). Mycolora of seed of common teasle (Disacus
fullonum) in Washington State. Paciic Northwest Fungi 2(6): 1–10.
Dugan FM, Roberts RG (1994). Morphological and reproductive aspects of
Cladosporium macrocarpum and C. herbarum from bing cherry fruits.
Mycotaxon 52: 513–522.
Dugan FM, Schubert K, Braun U (2004). Check-list of Cladosporium names.
Schlechtendalia 11: 1–103.
Ellis JB, Everhart BM (1894). New species of fungi from various localities.
Proceedings of the Academy of Natural Sciences of Philadelphia 46: 322–386.
Ellis JB, Everhart BM (1895). New species of fungi from various localities.
Proceedings of the Academy of Natural Sciences of Philadelphia 47: 413–441.
Ellis MB (1963). Dematiaceous hyphomycetes. V. Mycological Papers 93: 1–33.
Ellis MB (1968). Dematiaceous hyphomycetes IX. Spiropes and Pleurophragmium.
Mycological Papers 114: 1–44.
Ellis MB (1971). Dematiaceous hyphomycetes. CMI, Kew.
Ellis MB (1972). Dematiaceous hyphomycetes. XI. Mycological Papers 131: 1–25.
Ellis MB (1976). More dematiaceous hyphomycetes. CMI, Kew.
Ellis MB, Ellis JP (1985). Microfungi on land plants. An identiication handbook.
MacMillan, New York.
Ellis MB, Ellis JP (1988). Microfungi on miscellaneous substrates. An identiication
handbook. Croom Helm, London, and Timber Press, Portland, Oregon.
Ellis MB, Holliday P (1972). Cladosporium cucumerinum. CMI Descriptions of
Pathogenic Fungi and Bacteria No. 348.
Ellis MB, Waller JM (1974). Mycosphaerella macrospora (conidial state:
Cladosporium iridis). CMI Descriptions of Pathogenic Fungi and Bacteria No.
435.
El-Morsy EM (2000). Fungi isolated from the endorhizosphere of halophytic plants
from the Red Sea Coast of Egypt. Fungal Diversity 5: 43–54.
Esfandiari E (1951). Neue Iranische Pilze. Sydowia 5: 366–370.
Fairman CE (1922). New or rare fungi from various localities. Proceedings of the
Rochester Acadamy of Science 6: 117–139.
Farneti R (1904). Le Volatiche e l’Atroia dei Frutti del Fico. Atti dell’Istituto Botanico
Dell’Università di Parvia, Nuova Serie, 8: 36–517.
Farr DF, Bills GF, Chamuris GP, Rossman AY (1989). Fungi on plants and plant
products in the United States. APS Press, St. Paul, MN.
Farr DF, Rossman AY, Palm ME, McCray EB (n.d.). Fungal Databases, Systematic
Botany & Mycology Laboratory, ARS, USDA. Retrieved March 13, 2003, from
http://nt.ars-grin.gov/fungaldatabases.
Farr ML (1973). An annotated list of Spegazzini’s fungus taxa (vol. 1). Bibliotheca
Mycologica 35(1): 1–823.
Fawcett HS (1910). Cladosporium citri Mass. and C. elegans Penz. confused.
Mycologia 2: 245–246.
Fawcett HS (1936). Citrus diseases and their control. McGraw-Hill, New York.
Ferraris T (1909). Osservazioni micologiche su specie del gruppo Hyphales
(Hyphomycetae). Annales Mycologici 7: 273–286.
Ferraris T (1912). Hyphales, Dematiaceae. Flora Italica Cryptogama, Pars I: Fungi,
Fasc. 8: 195–534.
374
Ferraris T (1914). Addenda ad Hyphales. Flora Italica Cryptogama, Pars I: Fungi,
Fasc. 13: 851–923.
Fisher FE (1967) Cladosporium leaf spot of Citrus in Florida. Plant Disease Reporter
51(12): 1070.
Flannigan B (2001). Microorganisms in indoor air. In: Microorganisms in Home and
Indoor Work Environments: Diversity, Health Impacts, Investigation and Control
(Flannigan B, Samson RA, Miller JD, eds). Taylor & Francis, London: 17–31.
French AM (1989). California Plant Disease Host Index. Californian Department of
Food Agriculture. Sacramento.
Fresenius JBGW (1850). Beiträge zur Mykologie 1. Heinrich Ludwig Brömmer
Verlag, Frankfurt.
Fresenius JBGW (1863). Beiträge zur Mykologie 3. Heinrich Ludwig Brömmer
Verlag, Frankfurt.
Friend RJ (1965). What is Fumago vagans? Transactions of the British Mycological
Society 48: 371–376.
Fries EM (1821). Systema mycologicum, Vol. 1. Ex Oficina Berlingiana, Lund.
Fries EM (1832). Systema mycologicum, Vol. 3. E. Moritz, Greifswald.
Fries EM (1849). Summa vegetabilium Scandinaviae. Sectio posterior. Uppsala.
Fuckel KWGL (1870, ‘1869’). Symbolae Mycologicae. Beiträge zur Kenntnis der
rheinischen Pilze. Jahrbücher des Nassauischen Vereins für Naturkunde
23–24: 1–459.
Fuentes-Davila G, Gabrielson RL (1996). Penetration and infection of spinach
(Spinacia oleracea L.) leaf tissues by Cladosporium variabile. Revista
Mexicana de Micología 12: 49–55.
Fuss M (1878). Systematische Aufzählung der in Siebenbürgen angegebenen
Cryptogamen. Archiv des Vereins für Siebenbürgische Landeskunde, N.F.,
14(2): 421–474.
Gardner MW (1925). Cladosporium spot on cowpea. Phytopathology 25: 453–462.
Georgescu CC, Tutunaru V (1958). Mikromycetenlora auf den Nadelhölzern in der
RVR. Revue de Biologie, Bucharest 3(1): 41–66.
Gerrits van den Ende AHC, Hoog GS de (1999). Variability and molecular diagnostics
of the neurotropic species Cladophialophora bantiana. Studies in Mycology 43:
151–162.
Goh TK, Hsieh WH (1990). Cercospora and similar fungi from Taiwan. Maw Chang
Book Company, Taipei.
Gola G (1930). L’erbario micologico de P.A. Saccardo, Catalogo. Atti della
Accademia Scientiica Veneto-Trentino-Istriana 21(supplimento i): 1–329.
Goetz J, Dugan FM (2006). Alternaria malorum: a mini-review with new records for
hosts and pathogenicity. Paciic Northwest Fungi 1(3):1–8.
Gonzáles-Fragoso DR (1920). Datos para la deuteromicetologia Catalana.
Memorias de la Real Academia Ciencias Barcelona, Ser. 3, 15(17): 429–467.
Gonzáles-Fragoso DR (1926). Hongos de España. Brotéria, Sèrie Botanica 22: 69.
Gonzáles-Fragoso DR (1927). Estudio systemático de los Hifales de la Flora
Española. Memorias de la Real Academia Ciencias Exactas Físicas y
Naturales de Madrid, 2a Serie, 6: 1–377.
Gregory CT (1919). Heterosporium leafspot of timothy. Phytopathology 9: 576–580.
Grüss J (1931). Ein neuer Rußthaupilz, Cladosporium circinalis n. spec.
Wochenschrift für Brauerei 48(7): 67–68.
Guo YL (2001). Imperfect fungi in the tropical areas of China III. Mycosystema 20:
464–468.
Hall K, Kavanagh JA (1984). Laboratiory studies on the growth and reproduction of
Cladosporium allii-cepae, the cause of leaf blotch of onion. Plant Patholology
33(2): 147–143.
Hall K, Kavanagh JA (1985). Light and scanning electron-microscope studies of
leaf blotch of onion caused by Cladosporium allii-cepae. Plant Pathology 34(1):
1–4.
Hallier E (1866). Die planzlichen Parasiten des menschlichen Körpers. Leipzig.
Hallier E (1868a). Mykologische Untersuchungen. Flora, Neue Reihe 26(19): 289–
301.
Hallier E (1868b). Parasitologische Untersuchungen bezüglich auf die planzlichen
Organismen bei Masern, Hungertypus, Darmtyphus, Blattern, Kuhpocken,
Schafpocken, Cholera Nostras etc. Leipzig.
Hammouda AM (1992). A new leaf spot of pepper caused by Cladosporium
oxysporum. Plant Disease 76(5): 536–537.
Hanzawa J (1914). Fusarium cepae, ein neuer Zwiebelpilz Japans, sowie einige
andere Pilze an Zwiebelplanzen. Mycologisches Centralblatt 5: 4–13.
Harada T, Mino Y. (1976). Some properties of p-coumarate decaboxylase from
Cladosporium phlei. Canadian Journal of Microbiology 22(9): 1258–1262.
Hariot PA, Karsten PA (1890). Fungi novi. Revue Mycologique (Toulouse) 12: 128–
133.
Hasija SK (1967). Additions to the fungi of Jabalpur (Madhya Pradesh) – VI. Indian
Phytopathology 19(4): 373–377.
Haubold EM, Aronson JF, Cowan DF, McGinnis MR, Cooper CR (1998a). Isolation
of fungal rDNA from bottlenose dolphin skin infected with Loboa loboi. Medical
Mycology 36: 263–267.
Hawksworth DL (1979). The lichenicolous hyphomycetes. Bulletin of the British
Museum (Natural History), Botany 6(3): 183–300.
the genuS Cladosporium
Hawksworth DL (1986). Fungal genera in urgent need of taxonomic work.
Microbiological Sciences 3: 58.
Hawksworth DL, Crous PW, Redhead SA, Reynolds DR, Samson RA, et al. (2011).
The Amsterdam Declaration on Fungal Nomenclature. IMA Fungus 2(1): 105–
112.
Hawksworth DL (2011). A new dawn for the naming of fungi: impacts of decisions
made in Melbourne in July 2011 on the future publication and regulation of
fungal names. IMA Fungus 2(2): 155–162.
He YH, Zhang ZY (2001). Taxonomy of Cladosporium in China. XXVI. Mycosystema
20(4): 469–470.
He YH, Zhang ZY (2002). Taxonomy of Cladosporium in China. XXVII. Mycosystema
21(1): 21–22.
Hennebert GL, Sutton BC (1994). Unitary Parameters in Conidiogenesis. In:
Ascomycete Systematics. Problems and Perspectives in the Nineties
(Hawksworth DL, ed.). NATO ASI Series, vol. 296, New York, London: 65–76.
Heuchert B, Braun U (2006). On some dematiaceous lichenicolous hyphomycetes.
Herzogia 19: 11–21.
Heuchert B, Braun U, Schubert K (2005). Morphotaxonomic revision of fungicolous
Cladosporium species (hyphomycetes). Schlechtendalia 13: 1–78.
Hillis DM, Bull JJ (1993). An empirical test of bootstrapping as a method for assessing
conidence in phylogenetic analysis. Systematic Biology 42: 182–192.
Ho MH-M, Castañeda RF, Dugan FM, Jong SC (1999). Cladosporium and
Cladophialophora in culture: descriptions and an expanded key. Mycotaxon
72: 115–157.
Holliday P, Mulder JL (1976). Fulvia fulva. CMI Descriptions of Pathogenic Fungi
and Bacteria No. 487.
Holcomb E. (1989). First report of C. colocasiae on Taro in the United States. Plant
Disease 73: 938.
Holmgren PK, Holmgren NH, Barnett LC (1990). Index Herbariorum, Part 1: The
Herbaria of the World. 8th ed. New York Botanical Garden, New York.
Hoog GS de, Gerrits van den Ende AHG (1998). Molecular diagnostics of clinical
strains of ilamentous Basidiomycetes. Mycoses 41: 183–189.
Hoog GS de, Guarro J, Gené J, Figueras MJ (2000). Atlas of clinical fungi, 2nd ed.
CBS, Utrecht and Universitat rovira I virgili, Reus.
Hoog GS de, Guého E, Masclaux F, Gerrits van den Ende AHG, Kwon-Chung
KJ, McGinnis MR (1995). Nutritional physiology and taxonomy of humanpathogenic Cladosporium-Xylohypha species. Journal of Medical and
Veterinary Mycology 33: 339–347.
Hoog GS de, Hermanides-Nijhof EJ (1977). The black yeasts and allied
hyphomycetes. Studies in Mycology 15: 1–222.
Hoog GS de, Yurlova NA (1994). Conidiogenesis, nutritional physiology and
taxonomy of Aureobasidium pullulans and Hormonema. Antonie van
Leeuwenhoek Journal of Microbiology and Serology 65: 41–54.
Hughes SJ (1953). Conidiophores, conidia and classiication. Canadian Journal of
Botany 31: 577–659.
Hughes SJ (1958). Revisiones hyphomycetum aliquot cum appendice de nominibus
rejiciendis. Canadian Journal of Botany 36: 727–836.
Inacio J, Pereira P, Cavalho M de, Fonseca A, Amaral-Collaco MT, Spencer-Martins
I (2002). Estimation and diversity of phylloplane mycobiota on selected plants
on a Mediterranean-type ecosystem in Portugal. Microbial Ecology 44(4):
344–353.
Islam M, Hasin F (2000). Studies on phylloplane mycolora of Amaranthus viridis L.
National Academy Science Letters, India 23(9–10): 121–123.
Jaap O von (1902). Abhandlungen. Schriften des Naturwissenschaftlichen Vereins
Schleswig-Holstein 12: 346–347.
Jaap O von (1907). Beiträge zur Pilzlora der Schweiz. Annales Mycologici 5:
246–272.
Jaczewski AA (1929). Bolezni korobochek i bolokna khlobchatnika. Khlopkovoe
Delo 1929(5–6): 548–577.
Jaczewski AA (1931). Bolezni Khlopchatnika. 3. Kladosporioz. Trudy po Prikladnoi
Botanike, Genetiki i Selektsii 24(5): 180–182.
Jager de ES, Wehner FC, Karsten L (2001). Microbial ecology oft he mango
phylloplane. Micribial Ecology 42(2): 201–207.
Järva L, Parmasto E (1980). Eesti seente koondnimestik (List of Estonian Fungi).
Scripta Mycologica 7: 5–331. Tartu.
Jenkins AE (1925). The Citrus scab fungus. Phytopathology 15: 99–104.
Johan-Olsen O (1897). Zur Pleomorphismusfrage. Centralblatt für Bakteriologie,
Parasitenkunde und Infektionskrankheiten, Zweite Abtheilung 3: 273–284.
Jordan MM, Burchill RT, Maude RB (1990a). Epidemiology of Cladosporium allii
and Cladosporium allii-cepae, leaf blotch pathogens of leek and onion. 1.
Production and release of conidia. Annals of Applied Biology 117(2): 313–326.
Jordan MM, Burchill RT, Maude RB (1990b). Epidemiology of Cladosporium allii and
Cladosporium allii-cepae, leaf blotch pathogens of leek and onion. 2. Infection
of host plants. Annals of Applied Biology 117(2): 327–336.
Jordan MM, Maude RB, Burchill RT (1986). Development of the teleomorph
(Mycosphaerella allii-cepae sp. nov.) of Cladosporium allii-cepae (leaf blotch of
onion). Transactions of the British Mycological Society 86: 387–392.
www.studiesinmycology.org
Jordan MM, Maude RB, Burchill RT (1990a). Sources, survival and transmission of
Cladosporium allii and Cladosporium allii-cepae, leaf blotch pathogens of leek
and onion. Plant Pathology 39(2): 237–241.
Jordan MM, Maude RB, Burchill RT (1990b). Tests of fungicides for the control of
leaf blotch diseases – Cladosporium allii on leek and Cladosporium allii-cepae
on onion. Crop Protection 9(5): 367–370.
Kamal (2010). Cercosporoid fungi of India. Dehra Dun.
Karsten PA, Roumeguère C, Hariot P (1890). Fungilli novi. Revue Mycologique
(Toulouse) 12: 79–80.
Katoh K, Misawa K, Kuma K, Miyata T (2002). MAFFT: a novel method for rapid
multiple sequence alignment based on fast Fourier transform. Nucleic Acids
Research 30: 3059–3066.
Katsuki S (1951). Notes on some new or noteworthy fungi in Kyushu (2). Kyushu
Agricultural Research 8: 83–84.
Kendrick WB (1961). Hyphomycetes of conifer leaf litter. Hormodendrum
staurophorum sp. nov. Canadian Journal of Botany 39: 833–835.
Khan AZMN, Shamsi S (1986). Hyphomycetes from Bangladesh. Bangladesh
Journal of Botany 15(2): 111–121.
Khan SA, Kamal M (1962). A new species of Cladosporium on Heterophragma
adenophyllum Seem. Mycopathologia et Mycologia Applicata 18(4): 246–247.
Khan SA, Kamal MA (1974). Additions to the parasitic fungi of West Pakistan.
Mycopathologia et Mycologia Applicata 52(1): 33–34.
Khan SN, Misra BM (1999). A new Cladosporium leaf spot of Albizia. Indian Forester
125(7): 745–746.
Kiehr M, Anderson F, Azpilicueta A, Delhey R (1997). First record of onion leaf blotch
(Mycosphaerella allii-cepae) in Argentina. Bulletin OEPP/EPPO Bulletin 27:
255–257.
Kim JJ, Kang S-M, Choi Y-S, Kim G-H (2007). Microfungi potentially disiguring CCAtreated wood. International Biodeterioration & Biodegradation 60: 197–201.
Kirk PM (1986a). Cladosporium allii. CMI Descriptions of Plant Pathogenic Fungi
and Bacteria No. 841.
Kirk PM (1986b). Cladosporium allii-cepae. CMI Descriptions of Plant Pathogenic
Fungi and Bacteria No. 842.
Kirk PM (2003). Authors of fungal names. CABI Bioscience. www.indexfungorum.
org/Authorsof FungalNames.htm.
Kirk PM, Cannon PF, David JC, Stalpers JA (2001). Dictionary of the Fungi, 9th ed.
Wallingford. CAB International.
Kirk PM, Crompton JG (1984). Pathology and taxonomy of Cladosporium leaf blotch
of onion (Allium cepa) and leek (A. porrum). Plant Pathology 33: 317–324.
Kohlmeyer J (1962). Index alphabeticus Klotzschii et Rabenhorstii herbarii
mycologici. Beihefte zur Nova Hedwigia 4: 1–231.
Koike SK, Baameur A, Groenewald JZ, Crous PW (2011). Cercosporoid leaf
pathogens from whorled milkweed and spineless saflower in California. IMA
Fungus 2: 7–12.
Koshkelova EN (1977). Mikromitsety Yuzhnogo Turkmenistana tom 1. Ashkhabad:
Ilim.
Koshkelova EN, Frolov IP (1973). Microlora of Kopet-Dag lowland and Central
Karakum (micromycetes). Ylym, Ashkhabad, 194 pages.
Krangauz RA (1970). K sistematike gribov roda Cladosporium Fr. Trudy
Vsesoyuznogo Nachno-Issledovatel´skogo Instituta Zashchity Rastenii 29:
5–13.
Kumar S, Singh R, Kumar Pal V, Upadhyaya PP, Agarwal DK (2007). Additions
to new species of foliicolous hyphomycetes from North-eastern U.P. Indian
Phytopathology 60(3): 350–355.
Kumaresan V, Suryanarayanan TS (2002). Endophyte assemblage in young,
mature and senescent leaves of Rhizophora apiculata: evidence for the role of
endophytes in mangrove litter degeneration. Fungal Diversity 9: 81–91.
Kupka T (1918). Reliquiae Opizianae. Eine Revision Opiz’scher Pilze auf Grund des
Originalmaterial. Oesterreichische Botanische Zeitschrift 67: 156–165.
Kwon JH, Kang S.W, Park CS (1999). Occurrence of eggplant scab caused by
Cladosporium cucumerinum in Korea. The Plant Pathology Journal 15(6):
345–347.
Kwon JH, Kang SW, Park CS (2000). Occurrence of sword bean scab caused by
Cladosporium cucumerinum in Korea. Mycobiology 28: 54–56.
Kwon-Chung KJ, Bennett JE (1992). Medical mycology. Lea & Febiger, Philadelphia.
Lagière R (1945–1946). Étude de quelque Cladosporium. Annales de l’Ecole
Nationale d’Agriculture de Grignon, Série 3, 5: 147–160.
Lamboy JS, Dillard HR (1997). First report of a leaf spot caused by Cladosporium
oxysporum on greenhouse tomato. Plant Disease 81(2): 228.
Laundon GF (1970). Records of fungal plant diseases in New Zealand. New Zealand
Journal of Botany 8: 51–66.
Lawrence GHM, Buchheim AFG, Daniels GS, Dolezal H (1968). BotanicoPeriodicum-Huntianum. Hunt Botanical Library. Pittsburgh, PA.
Lenné JM (1990). World List of Fungal Diseases of Tropical Pasture Species.
Phytopathological Papers 31: 1–192.
Levetin E, Dorseys K (2006). Contribution to leaf surface fungi to the air spora.
Aerobiologia 22(1): 2–12.
375
BenSch et al.
Lind J (1913). Danish fungi as represented in the herbarium of E. Rostrup.
Copenhagen.
Lind J (1934). Studies on the geographical distribution of artic circumpolar
micromycetes.
Kongelige
Danske
Videnskabernes
Selskabs
Naturvidenskabelige og Mathematiske Afhandlinger 11(2): 1–152.
Lindau G (1907). Dr. L. Rabenhorst’s Kryptogamen-Flora von Deutschland,
Oesterreich und der Schweiz. Zweite Aulage. Erster Band: Die Pilze
Deutschlands, Österreichs und der Schweiz. VIII. Abteilung: Fungi imperfecti:
Hyphomycetes (erste Hälfte), Mucedinaceae, Dematiaceae (Phaeosporae und
Phaeodidymae). Leipzig.
Lindau G (1910). Dr. L. Rabenhorst’s Kryptogamen-Flora von Deutschland,
Oesterreich und der Schweiz. Zweite Aulage. Erster Band: Die Pilze
Deutschlands, Österreichs und der Schweiz. IX. Abteilung: Fungi imperfecti:
Hyphomycetes (zweite Hälfte), Dematiaceae (Phaeophragmiae bis
Phaeostaurosporae), Stilbaceae, Tuberculariaceae, sowie Nachträge,
Nährplanzenverzeichnis und Register. Leipzig.
Linder DH (1947). Botany of the Canadian eastern arctic, Part II. Thallophyta and
Bryophyta, 4. Fungi. Bulletin of the National Museum of Canada 97: 234–297.
Link HF (1809). Observationes in ordines plantarum naturales. Dissertatio I,
complectens Anandrarum ordines Epiphytas, Mucedines, Gastromycos et
Fungos. Der Gesellschaft natur-forschender Freunde zu Berlin Magazin für die
neuesten Entdeckungen in der gesammten Naturkunde 3: 3–42.
Link HF (1816). Observationes in ordines plantarum naturales. Dissertatio II, sistens
nuperas de Mucedinum et Gastromycorum ordinibus observationes. Der
Gesellschaft naturforschender Freunde zu Berlin Magazin für die neuesten
Entdeckungen in der gesammten Naturkunde 7: 25–45.
Link HF (1824). In: Willdenow CL: Species plantarum, Ed. 4, vol. 6(1). Berlin.
Liu YL, He YH, Zhang ZY (2000). Taxonomy of Cladosporium in China XXV.
Mycosystema 19(2): 169–171.
Liu YL, Zhang ZY (1998). A new species of Cladosporium. Plant Diseases and Their
Control: 101–102.
Lobik AI (1928). Materialy k mikologicheskoj lore Terskogo okruga. Bolezni Rastenii
17(3–4): 157–199.
Lu HJ, Liu YL, Zhang ZY (2003). Taxonomy of Cladosporium in China XXIX.
Mycosystema 22: 49–51.
Marcolongo I (1914). Intorno ad una alterazione della foglie di “Cycas revoluta”.
Rivista di Patologia Vegetale 7(1): 6–8.
Martius CFP von (1817). Flora Cryptogamica Erlangensis sistens vegetabilia e
classe ultima Linn. In agro Erlangensi hucusque detecta. Nürnberg.
Martyn EB (1945). A note on banana leaf speckle in Jamaica and some associated
fungi. Mycological Papers 13: 1–5.
Masclaux F, Guého E, Hoog GS de, Christen R (1995). Phylogenetic relationship of
human-pathogenic Cladosporium (Xylohypha) species inferred from partial LS
rRNA sequences. Journal of Medical and Veterinary Mycology 33: 327–338.
Mason EW, Ellis MB (1953). British species of Periconia. Mycological Papers 56:
1–127.
Massee GE (1887). Disease of Colocasia in Jamaica. Journal of the Linnean
Society of London 24: 45–49.
Massee GE (1910). Diseases of cultivated plants and trees. London: Duckworth
& Co.
Mathur M, Mukerji KG (1981). Antagonistic behaviour of Cladosporium spongiosum
against Phyllactinia dalbergiae on Dalbergia sissoi. Angewandte Botanik 55:
75–77.
Matsushima T (1975). Icones Microfungorum a Matsushima Lectorum. Kobe.
Matsushima T (1980). Matsushima Mycological Memoirs No. 1. Saprophytic
Microfungi from Taiwan, Part 1. Hyphomycetes. Matsushima Fungus Collection,
Kobe, Japan.
Matsushima T (1983). Matsushima Mycological Memoirs No. 3. Matsushima Fungus
Collection, Kobe, Japan.
Matsushima T (1985). Matsushima Mycological Memoirs No. 4. Matsushima Fungus
Collection, Kobe, Japan.
Matsushima T (1993). Matsushima Mycological Memoirs No. 7. Matsushima Fungus
Collection, Kobe, Japan.
McAlpine D (1899). Fungus diseases of Citrus trees in Australia. Melbourne.
McAlpine D (1902). Fungus diseases of stone-fruit trees in Australia. Department of
Agriculture, Victoria, Australia.
McGinnis MR, Ajello L (1982). A note on Sporotrichum gougerotii Matruchot 1910.
Mycotaxon 16: 232–238.
McGinnis MR, Borelli D (1981). Cladosporium bantianum and its synonym
Cladosporium trichoides. Mycotaxon 18: 127–136.
McGinnis MR, Padhye AA (1978). Cladosporium castellani is a synonym of Stenella
araguata. Mycotaxon 7: 415–418.
McKemy JM, Gudauskas RT, Morgan-Jones G, Turner DL (1993). Leaf blight of
Lespedeza spp. caused by Cladosporium vignae. Plant Disease 77(12): 1263.
McKemy JM, Morgan-Jones G (1990). Studies in the genus Cladosporium sensu
lato II. Concerning Heterosporium gracile, the causal organism of leaf spot
disease of Iris species. Mycotaxon 39: 425–440.
376
McKemy JM, Morgan-Jones G (1991a). Studies in the genus Cladosporium
sensu lato III. Concerning Cladosporium chlorocephalum and its synonym
Cladosporium paeoniae, the causal organism of leaf-blotch of peony.
Mycotaxon 41: 135–146.
McKemy JM, Morgan-Jones G (1991b). Studies in the genus Cladosporium sensu
lato IV. Concerning Cladosporium oxysporum, a plurivorous predominantly
saprophytic species in warm climates. Mycotaxon 41: 397–405.
McKemy JM, Morgan-Jones G (1991c). Studies in the genus Cladosporium sensu
lato V. Concerning the type species Cladosporium herbarum. Mycotaxon 42:
307–317.
McKemy JM, Morgan-Jones G (1992). Studies in the genus Cladosporium sensu
lato. VII. Concerning Cladosporium cucumerinum, causal organism of crown
blight and scab or gummosis of cucurbits. Mycotaxon 43: 163–170.
McTaggart AR, Shivas RG, Braun U (2007). Annellosympodia orbiculata gen. et
sp. nov. and Scolecostigmina lagellariae sp. nov. from Australia. Australasian
Plant Pathology 36: 573–579.
Meklin T, Haugland RA, Reponen T, Varma M, Lummus Z, Bernstein D, Wymer
LJ, Vesper SJ (2004). Quantitative PCR analysis of house dust can reveal
abnormal mold conditions. Journal of Environmental Monitoring 6: 615–620.
Mendes MAS, Silva VL da, Dianese JC, Ferreira MASV, Santos CEN dos, Neto EG,
Urben AF, Castro C (1998). Fungos em plantas no Brasil. EMBRAPA. Brasília,
D.F.
Menkis A, Vasiliauskas R, Taylor AFS, Stenlid J, Finlay R (2005). Fungal
communities in mycorrhizal roots of conifer seedlings in forest nurseries under
different cultivation systems, assessed by morphotyping, direct sequencing
and mycelial isolation. Mycorrhiza 16: 33–41.
Merrill ED (1914). An enumeration of the plants of Guam. Philippine Journal of
Science 9: 17–96.
Milko AA, Dunaev AS (1986). De specie nova Cladosporii (Cladosporium
strumelloideum Milko et Dunaev) et novitatibus ad Dactylellam submersam
(Ingold) Nilsson pertinentibus notula. Novosti Sistematiki Nizshikh Rastenii 23:
134–138
Minoura K (1966). Taxonomic studies on Cladosporia (IV). Morphological properties
(Part II). Journal of Fermentation Technology 44: 137–149.
Minter DW, Kirk PM, Sutton BC (1982). Holoblastic phialides. Transactions of the
British Mycological Society 79: 75–93.
Moesz G von (1932). Neue Pilze aus Lettland. II. Mitteilung. Magyar Botanikai
Lapok 31: 37–43.
Montagne JPFC (1849). Sixième centurie de plantes cellulaires nouvelles, faut
indigènes qu’exotique, Décades VIII. Annales des Sciences Naturelles
Botanique, 3. Sér., 12: 285–320.
Montagne JPFC (1857). Huitième Centurie de Plantes Cellulaires Nouvelles, tant
indigenes qu’exotiques, Decades VI et VII (1). Annales des Sciences Naturelles
Botanique, 4. Sér., 8: 285–310.
Morgan-Jones G (1977). Notes on Hyphomycetes XIX. Cladosporium leprosum sp.
nov. and Cladosporium nigrellum. Mycotaxon 6: 1–5.
Morgan-Jones G, Jacobsen BJ (1988). Notes on hyphomycetes. LVIII. Some
dematiaceous taxa, including two undescribed species of Cladosporium,
associated with biodeterioration of carpet, plaster and wallpaper. Mycotaxon
32: 223–236.
Morgan-Jones G, Kendrick B (1972). Notes on Hyphomycetes. III. Redisposition
of six species of Exosporium. Canadian Journal of Botany 50(9): 1817–1824.
Morgan-Jones G, McKemy JM (1990). Studies in the genus Cladosporium sensu
lato I. Concerning Cladosporium uredinicola, occurring on telial columns of
Cronartium quercuum and other rusts. Mycotaxon 39: 185–202.
Morgan-Jones G, McKemy JM (1992). Studies in the genus Cladosporium sensu
lato VI. Concerning Cladosporium vignae, causal organism of leaf and pod spot
of cowpea (Vigna unguiculata) and leaf blight of Lespedeza bicolor. Mycotaxon
43: 9–20.
Moricca S, Ragazzi A, Mitchelson KR (1999). Molecular and conventional detection
and identiication of Cladosporium tenuissimum on two-needle pine rust
aeciospores. Canadian Journal of Botany 77: 339–347.
Mulder JL (1982). New species and combinations in Stenella. Transactions of the
British Mycological Society 79: 469–478.
Mułenko W, Schubert K, Kozlowska M (2004). Cladosporium galii sp. nov. on Galium
odoratum from Poland. Mycotaxon 90(2): 271–274.
Mullins J (2001). Microorganisms in outdoor air. In: Microorganisms in Home and
Indoor Work Environments: Diversity, Health Impacts, Investigation and Control
(Flannigan B, Samson RA, Miller JD, eds). Taylor & Francis, London: 3–16.
Nannizzi A (1929). Note micologiche. Atti della Reale Accademia dei Fisiocritici in
Siena, Series 10, 4: 87–96.
Nannizzi A (1934). Repertorio sistematico dei miceti dell’uomo e degli animali.
Trattato de Micopatologia Umana, Vol. 4, Siena.
Nechitsch A (1904). Sur les ferments de deux levains de l’inde le Mucor praini et le
Dematium chodati. Institut de Botanique, Université de Genève, Sér. 6, 5: 1–36.
Nees von Esenbeck CG (1817). Das System der Pilze und Schwämme. Ein
Versuch. Band 1. Würzburg.
the genuS Cladosporium
Nieves-Rivera ÁM, Rodríguez NJ, Dugan FM, Zaidi BR, Williams Jr EH (2006).
Characterization of Cladosporium oxysporum and C. sphaerospermum using
polyaromatic hydrocarbons (PAHs) as their sole carbon source in tropical
coastal seawater. In: Modern multidisciplinary applied microbiology: Exploiting
microbes and their interactions (Mendez-Vilas A, ed.). Wiley-VCH, Weinheim,
Germany: 483–487.
Noelli A (1917). Micromiceti del Piemonte. Nuovo Giornale Botanico Italiano, Nuova
Serie 24(3): 195.
O’Donnell K, Kistler HC, Cigelnik E, Ploetz RC (1998). Multiple evolutionary
origins of the fungus causing Panama disease of banana: concordant
evidence from nuclear and mitochondrial gene genealogies. Proceedings
of the National Academy of Sciences of the United States of America 95(5):
2044–2049.
Ogundero VW (1986). The fungal lora of post-harvest grains of Sorghum guineense
Stapf and their importance in pathogenicity. Journal of Basic Microbiology
26(6): 359–363.
Osipjan LL (1975). Grifal´nye griby. Mikrolora Armanskoj SSR. Tom III. Erevan.
Oudemans CAJA (1892). Contribution à la lore mycologique de Pays-Bas, sé. 14.
Nederlandsch Kruidkundig Archief, Ser. 2, 6(1): 1–65.
Oudemans CAJA (1902a). Beiträge zur Pilzlora der Niederlande. Beiheft
Botanisches Centralblatt 11: 538.
Oudemans CAJA (1902b). Contributions à la lore mycologique des Pays-Bas.
XVIII. Nederlandsch Kruidkundig Archief, Ser. 3, 2(3): 633–781.
Oudemans CAJA (1919–1924). Enumeratio Systemica Fungorum. Vol. 1 (1919);
Vol. 2 (1920); Vol. 3 (1921); Vol. 4 (1923); Vol. 5 (index, 1924). Hague comitum,
apud M. Nijhoff.
Padwick GW (1950). Manual of rice diseases. CMI, Kew.
Pal AK, Purkayastha RP (1992). Parasitic fungi from Indian mangrove. Journal of
Mycopathological Research 30(2): 175.
Park HG, Managbanag JR, Stamenova EK, Jong SC (2004). Comparative analysis
of common indoor Cladosporium species based on molecular data and conidial
characters. Mycotaxon 89(2): 441–451.
Partridge EC, Baker WA, Morgan-Jones G (2001a). Notes on hyphomycetes. LXXIX.
Concerning the Acladium-Alysidium-Haplotrichum complex; nomenclature and
taxonomic considerations, with redescriptions of respective type species.
Mycotaxon 77: 201–214.
Partridge EC, Baker WA, Morgan-Jones G (2001b). Notes on Hyphomycetes.
LXXXIII. Castanedaea, a new genus in which to accommodate Alysidium
minus. Mycotaxon 78: 175–180.
Partridge EC, Morgan-Jones G (2002). Notes on hyphomycetes LXXXVIII: New
genera in which to classify Alysidium resinae and Pycnostysanus azaleae, with
a consideration of Sorocybe. Mycotaxon 83: 335–352.
Partridge EC, Morgan-Jones G (2003). Notes on hyphomycetes. XC.
Fusicladosporium, a new genus for Cladosporium-like anamorphs of Venturia,
and the pecan scab-inducing fungus. Mycotaxon 85: 357–370.
Pasqualetti M, Rambelli A, Mulas B, Tempesta S (2005). Identiication key and
description of Mediterranean maquis litter microfungi. Bocconea 18: 5–176.
Patouillard N (1892). Exploration Scientiiques de la Tunisie. Énumération des
champignons observes en Tunisie. Paris: Imprimerie Internationale.
Patterson W (1900). New species of fungi. Bulletin of the Torrey Botanical Club 27:
282–286.
Penzig AJO (1882). Funghi agrumicoli. Michelia 2(8): 385–508.
Persoon CH (1794). Neuer Versuch einer systematischen Eintheilung der
Schwämme. Neues Magazin für die Botanik in ihrem ganzen Umfange 1:
63–128.
Persoon CH (1822). Mycologia europaea. Sectio prima. Erlangen.
Pister DH (1977). Annotated index to fungi described by N. Patouillard. Contributions
to the Reed Herbarium 25: 1–211.
Pidoplichko NM (1953). Gribnaja Flora Grubych Kormov. Izdatel’stvo Akademii
Nauk Ukrainskoj SSR, Kiev.
Pidoplichko NM, Deniak VI (1938). Materialy k izucheniju roda Cladosporium.
Mikrobiologichnii Zhurnal 5(2): 182–194.
Politis JC (1935). Contribution à l’Étude des Champignons de l’Attique. Praktika tēs
Akadēmias Athēnōn 4: 1–44.
Potin O, Veignie E, Rain C (2004). Biodegradation of polycyclic aromatic
hydrocarbons (PAHs) by Cladosporium sphaerospermum isolated from an
aged PAH contaminated soil. FEMS Microbiology Ecology 51: 71–78.
Powell JM (1971). Fungi and bacteria associated with Cronartium comandrae on
lodgepole pine in Alberta. Phytoprotection 52: 45–51.
Prasil K, Hoog GS de (1988). Variability in Cladosporium herbarum. Transactions of
the British Mycological Society 90(1): 49–54.
Prenafeta-Boldú FX, Kuhn A, Luykx DMAM, Anke H, Groenestijn JW van, Bont JAM
de (2001). Isolation and characterization of fungi growing on volatile aromatic
hydrocarbons as their sole carbon and energy source. Mycological Research
4: 477–484.
Preuss cgt (1848). Die Pilze Deutschlands, Bd 6: Fungi imperfecti, Hefte 25/26. In:
Deutschlands Flora Abt. 3. (Sturm J, ed.). Nürnberg.
www.studiesinmycology.org
Preuss CGT (1851). Uebersicht untersuchter Pilze, besonders aus der Umgebung
von Hoyerswerda. Linnaea 25: 723–742.
Qin Y, Zhang ZY (1999). Taxonomy of Cladosporium in China XII. A new species on
Circaea. Mycosystema 18(2): 135–136.
Rabenhorst GL (1844). Deutschlands Kryptogamen-Flora oder Handbuch zur
Bestimmung der kryptogamischen Gewächse Deutschlands, der Schweiz,
des Lombardisch-Venetianischen Königreiches und Istriens. Band I. (Pilze).
Leipzig.
Ragavendra, AK (2012). Fungal endophytes: New insights into their community
assembly and defense mutualism. PhD thesis, University of Idaho.
Rambaut A (2002). Sequence Alignment Editor. Version 2.0. Department of Zoology,
University of Oxford, Oxford.
Ranojevic N (1910). Zweiter Beitrag zur Pilzlora Serbiens. Annales Mycologici 8:
347–402.
Rao SC (1988). A new disease on areca nut stem caused by Cladosporium
spongiosum. Current Science 57: 1074.
Rao R, Baheker VS (1964). Fungi on Cycas revoluta Thunb. Mycopathologia 23(4):
266–268.
Raybaud L (1923). Le Cladosporium lauri parasite de la Cochenille du Laurier.
Congres Pathologie Vegetale (Cent. Pasteur), Strausbourg.
Rayner RW (1970). A mycological colour chart. CMI and British Mycological Society.
Kew, Surrey, England.
Rebrikova NL, Sizova TP (1978). Cladosporium brevi-catenulatum Rebr. et Sizova
sp. nov. in textis musaeo conservatis inventum. Novosti Sistimatiki Nizshikh
Rastenii 15: 137–139.
Rehner SA, Samuels GJ (1994). Taxonomy and phylogeny of Gliocladium analysed
from nuclear large subunit ribosomal DNA sequences. Mycological Research
98: 625–634.
Reichardt HW (1878). Kleinere Mittheilungen aus dem botanischen Laboratorium
des K.K. a. ö. Universitäts-Professors Dr. H.W. Reichardt. VI. Über einige neue
oder seltene Pilze der österreichischen Flora. Verhandlungen der KaiserlichKöniglichen Zoologisch-Botanischen Gesellschaft in Wien 27: 841–845.
Reichert I (1921). Die Pilzlora Ägyptens. Botanische Jahrbücher für Systematik,
Planzengeschichte und Planzengeographie 56: 598–727.
Reissek S (1851). Entwicklung-Geschichte des Thieres und der Planze durch
Urzeugung. Sitzungsberichte der Kaiserlichen Akademie der Wissenschaften,
Mathematisch-Naturwissenschaftliche Classe 7(2): 334–341.
Riedl H von (1968). Cladosporium herbarum Link ex Fr. und Cladosporium murorum
Petr. Sydowia 20: 331–338.
Riedl H von, Ershad D (1977). Mykologische Ergebnisse einer Sammelreise in den
Iran im Frühjahr 1974. – I. Sydowia 29(1–6): 155–169.
Riesen T, Sieber T (1985). Endophytic fungi in winter wheat (Triticum aestivum L.).
Swiss Federal Institute of Technology, Zürich.
Ritschel A (2001). Taxonomische Revision der Gattungen Pollaccia und Spilocaea
(Hyphomycetes, Venturia-Anamorphen). Diplom-Arbeit, Martin-LutherUniversität, Halle.
Roberts RG, Robertson JA, Hanlin RT (1986). Fungi occurring in the achenes of
sunlower (Helianthus annuus). Canadian Journal of Botany 64: 1964–1971.
Roquebert MF (1981). Analyse des phénoménes pariétaux au cours de la
conidiogenése chez quelques champignon microscopiques. Mémoires du
Muséum National d’Histoire Naturelle, Série B, Botanique, 28: 3–79.
Saccardo PA (1880). Fungi Gallici. Series II. Michelia 2: 39–135.
Saccardo PA (1881). Fungi Gallici. Series III. Michelia 2: 302–371.
Saccardo PA (1882). Sylloge Fungorum vol. 1. Padova.
Saccardo PA (1883). Sylloge Fungorum vol. 2. Padova.
Saccardo PA (1883–1884). Miscellanea Mycologica. I. Fungi gallici, Ser. V. Atti del
Reale Istituto Veneto di Scienze, Lettere ed Arti, Ser. 6, 2(3): 435–463.
Saccardo PA (1884). Sylloge Fungorum vol. 3. Padova.
Saccardo PA (1886). Sylloge Fungorum vol. 4. Padova.
Saccardo PA (1891). Sylloge Fungorum vol. 9. Padova.
Saccardo PA (1892). Sylloge Fungorum vol. 10. Padova.
Saccardo PA (1895). Sylloge Fungorum vol. 11. Padova.
Saccardo PA (1899). Sylloge Fungorum vol. 14 (Saccardo PA & Sydow P eds.).
Padova.
Saccardo PA (1902). Sylloge Fungorum vol. 16 (Saccardo PA & Sydow P eds.).
Padova.
Saccardo PA (1905). Notae mycologicae, Ser. V. Mycetes novi. Annales Mycologici
3: 165–171.
Saccardo PA (1906). Sylloge Fungorum vol. 18 (Saccardo PA & Saccardo D eds.).
Padova.
Saccardo PA (1910). Notae mycologicae. Annales Mycologici 8: 333–347.
Saccardo PA (1911). Sylloge Fungorum vol. 20 (Saccardo PA & Traverso JB eds.).
Padova.
Saccardo PA (1913a). Sylloge Fungorum vol. 22 (Saccardo PA & Trotter A eds.).
Padova.
Saccardo PA (1913b). Notae mycologicae, Ser. XV. Fungi ex Gallia, Germania,
Italia, Melita (Malta), Mexico, India, Japonica. Annales Mycologici 11: 14–21.
377
BenSch et al.
Saccardo PA (1915a). Fungi ex Insula Melita (Malta) lecti a Doct. A. Carauana-Gatto
et Doct. G. Borg annis MCMXIII et MCMXIV. Nuovo Giornale Botanico Italiano,
Nuova Serie 22: 24–76.
Saccardo PA (1915b). Notae Mycologicae. Annales Mycologici 13(2): 115–138.
Saccardo PA (1921). Notae mycologicae. Series XXIV. I. Fungi Singaporenses
Barkesiani. Bulletino dell’Orto Botanico della Regia Università di Napoli 6:
39–73.
Saccardo PA (1931). Sylloge Fungorum vol. 25 (Trotter A ed.). Avellino.
Saccardo PA (1972). Sylloge Fungorum vol. 26 (Trotter A ed., published by Cash K).
Johnson Reprint Corporation, New York, London.
Saccardo PA, Berlese AN (1884–1885). Miscellanea Mycologica, Series II. Atti del
Reale Istituto Veneto di Scienze, Lettere ed Arti, Ser. 6, 3(4): 711–742.
Sagdullaeva MSh, Kirgizbaeva KhM, Ramazanova SS, Gulyamova M, Fajzieva FKh
(1990). Flora gribov Usbekistana. T. 6. Gifal’nye griby (Dematiaceae). ‘Fan’
Publishing House, Taškent.
Samson RA, Houbraken JAMP, Summerbell RC, Flannigan B, Miller JD (2001).
Common and important species of Actinomycetes and fungi in indoor
environments. In: Microorganisms in Home and Indoor Work Environments:
Diversity, Health Impacts, Investigation and Control (Flannigan B, Samson RA,
Miller JD, eds). Taylor & Francis, London: 287–473.
Samson R, Reenen-Hoekstra ES van, Frisvad JC, Filtenborg O (2000). Introduction
to food- and airborne fungi, 6th ed. CBS, Utrecht.
Sartory A (1923). Blastospores. Champignons Parasites de l’Homme et des
Animaux 11: 727–788.
Săvulescu T (1951). Noutăţi din micolora Republicii Populare Române. Buletin
Ştiinţiic Accademia Republicii Populare Române 3(2): 211–227.
Sawada K (1931). Descriptive catalogue of Formosan fungi. Report of the
Government Research Institute Formosa 51: 112.
Sawada K (1958). Research of Fungi in the Tohoku District of Japan. IV. Fungi
imperfecti. Bulletin of the Government Forest Experimental Station, Meguro,
Tokyo 105: 35–137.
Sawada K (1959). Descriptive catalogue of the Taiwan (Formosa) fungi XI. Special
Publications, College of Agriculture, National Taiwan University 8: 211–277.
Schell WA (2003). Dematiaceous hyphomycetes. In: Pathogenic fungi in humans
and animals, 2nd ed. (Howard DH, ed.). Marcel Dekker, New York: 565–636.
Schoch C, Shoemaker RA, Seifert KA, Hambleton S, Spatafora JW, Crous PW
(2006). A multigene phylogeny of the Dothideomycetes using four nuclear loci.
Mycologia 98: 1041–1052.
Schoch CL, Crous PW, Groenewald JZ, Boehm EWA, Burgess TI, et al. (2009a). A
class-wide phylogenetic assessment of Dothideomycetes. Studies in Mycology
64: 1–15.
Schoch CL, Sung GH, López-Giráldez F, Townsend JP, Miadlikowska J, et al.
(2009b). The Ascomycota Tree of Life: a phylum-wide phylogeny clariies
the origin and evolution of fundamental reproductive and ecological traits.
Systematic Biology 58: 224–239.
Scholler M, Braun U, Ruhl G (2004). Fusicladium levieri, a fungal parasite of
persimmon, found in Indiana. Proceedings of the Indiana Academy of Science
112(2): 132–134 "2003".
Schubert K (2001). Taxonomische Revision der Gattung Fusicladium (Hyphomycetes,
Venturia-Anamorphen). Diplom-Arbeit, Martin-Luther-Universität, Halle.
Schubert K (2005a). Taxonomic revision of the genus Cladosporium s. lat. 3. A
revision of Cladosporium species described by J.J. Davis and H.C. Greene
(WIS). Mycotaxon 92: 55–76.
Schubert K (2005b). Morphotaxonomic revision of foliicolous Cladosporium species
(hyphomycetes). Ph.D. dissertation. Martin-Luther-University Halle-Wittenberg,
Germany. http://sundoc.bibliothek.uni-halle.de/diss-online/05/05H208/index.htm.
Schubert K, Braun U (2002a). Fusicladium effusum. IMI Descriptions of Fungi and
Bacteria 152, No. 1514.
Schubert K, Braun U (2002b). Fusicladium romellianum. IMI Descriptions of Fungi
and Bacteria 152, No. 1517.
Schubert K, Braun U (2002c). Fusicladium subsessile. IMI Descriptions of Fungi and
Bacteria 152, No. 1519.
Schubert K, Braun U (2004). Taxonomic revision of the genus Cladosporium s. lat.
2. Cladosporium species occurring on hosts of the families Bignoniaceae and
Orchidaceae. Sydowia 56(2): 296–317.
Schubert K, Braun U (2005a). Taxonomic revision of the genus Cladosporium
s. lat. 1. Species reallocated to Fusicladium, Parastenella, Passalora,
Pseudocercospora and Stenella. Mycological Progress 4(2): 101–109.
Schubert K, Braun U (2005b). Taxonomic revision of the genus Cladosporium s.
lat. 4. Species reallocated to Asperisporium, Dischloridium, Fusicladium,
Passalora, Pseudoasperisporium and Stenella. Fungal Diversity 20: 187–208.
Schubert K, Braun U (2007). Taxonomic revision of the genus Cladosporium s. lat.
6. New species, reallocations to and synonyms of Cercospora, Fusicladium,
Passalora, Septonema and Stenella. Nova Hedwigia 84: 189–208.
Schubert K, Braun U, Groenewald JZ, Crous PW (2007a). Cladosporium leaf-blotch
and stem rot of Paeonia spp. caused by Dichocladosporium gen. nov. Studies
in Mycology 58: 95–104.
378
Schubert K, Braun U, Mułenko W (2006). Taxonomic revision of the genus
Cladosporium s. lat. 5. Validation and description of new species.
Schlechtendalia 14: 55–83.
Schubert K, Greslebin A, Groenewald JZ, Crous PW (2009). New foliicolous species
of Cladosporium from South America. Persoonia 22: 111–122.
Schubert K, Groenewald JZ, Braun U, Dijksterhuis J, Starink MS, Hill CF, Zalar P,
Hoog GS de, Crous PW (2007b). Biodiversity in the Cladosporium herbarum
complex (Davidiellaceae, Capnodiales), with standardisation of methods for
Cladosporium taxonomy and diagnostics. Studies in Mycology 58: 105–156.
Schubert K, Ritschel A, Braun U (2003). A monograph of Fusicladium s. lat.
(hyphomycetes). Schlechtendalia 9: 1–132.
Schwabe SH (1839). Flora Anhaltina. Tomus 2. Berlin.
Schweinitz LD von (1822). Synopsis fungorum Carolinae superioris. Fridericus
Schwäqrichen, Leipzig.
Schweinitz LD von (1832). Synopsis fungorum in America boreali media degentium.
Secundum observationes. Transactions of the Philosophic Society, N.S., 4(2):
141–316.
Seifert KA, Nickerson NL, Corlett M, Jackson ED, Louis-Seize G, Davies RJ
(2004). Devriesia, a new hyphomycete genus to accommodate heat-resistant,
cladosporium-like fungi. Canadian Journal of Botany 82: 914–926.
Seifert KA, Hughes SJ, Boulay H, Louis-Seize G (2007). Taxonomy, nomenclature
and phylogeny of three cladosporium-like hyphomycetes, Sorocybe resinae,
Seifertia azalea and the Hormoconis anamorph of Amorphotheca resinae.
Studies in Mycology 58: 235–245.
Sharma CD, Gadpandey KK, Firdousi SA, Rai AN, Vyas KM (1998). Three new
species of Cladosporium from Madhya Pradesh, India. Indian Phytopathology
51(2): 152–160.
Sharma IK, Heather WA (1981). Hyperparasitism of Melampsora laricis-populina
by Cladosporium herbarum and Cladosporium tenuissimum. Indian
Phytopathology 34: 395–397.
Sharma IK, Heather WA (1988). Light and electron microscope studies on
Cladosporium tenuissimum, mycoparasitic on poplar leaf rust, Melampsora
laricis-populina. Transaction of the British Mycological Society 90(1): 125–131.
Shaw CG (1973). Host fungus index for the Paciic Northwest – I. Hosts. Bulletin of
the Washington State Agricultural Experimentation Station 765: 1–121.
Sheta W (1996). Detection of Cladosporium uredinicola in pustules of
Chrysanthemum White Rust (Puccinia horiana). Plant Disease 80: 599.
Shin H-D (1995). Leaf blotch of Allium istulosum caused by Cladosporium alliicepae. Korean Journal of Plant Pathology 11(1): 91–93.
Shin H-D, Braun U (1995). Cladosporium alliicola sp. nov. on Allium victorialis var.
platyphyllum. Korean Journal of Mycology 23: 139–143.
Shin H-D, Lee HT, Im DJ (1999). Occurrence of German Iris Leaf Spot caused by
Cladosporium iridis in Korea. The Plant Pathology Journal 15(2): 124–126.
Shvartsman SR, Vasyagina MP, Byzova ZM, Filimonova NM (1975). Nesovershennye
griby – Fungi imperfecti (Deuteromycetes). Monilial’nye – Moniliales. Vol. 8,
part 2, of Series “Flora sporovykh rastenij Kazakhstana”. ‘Nauka’ Publishing
House, Alma-Ata.
Silva M da, Minter DW (1995). Fungi from Brazil recorded by Batista and co-workers.
Mycological Papers 169: 1–585.
Simmons EG (2007). Alternaria. An identiication manual. CBS Biodiversity Series
6: 1–775.
Singh RA, Shankar G (1971). Some parasitic fungi on Piper betle, Varanasi, Uttar
Pradesh. Mycopathologia et Mycologia Applicata 43: 109–115.
Sivanesan A (1974). Venturia carpophila. CMI Descriptions of Pathogenic Fungi and
Bacteria, No. 402.
Sivanesan A (1984). The bitunicate Ascomycetes and their anamorphs. Cramer
Verlag, Vaduz.
Sivanesan A, Holliday P (1981). Venturia cerasi. CMI Descriptions of Pathogenic
Fungi and Bacteria, No. 706.
Snyder WC (1934). A leaf, stem, and pod spot of pea caused by a species of
Cladosporium. Phytopathology 24: 890–905.
Sorokin NW (1893). O njekotorych boljesnjach winograda i drugich rastenij
Kawkasskago kraja. (Über einige Krankheiten des Weinstocks etc. im
Kaukasus). Zeitschrift für Planzenkrankheiten 3: 153–161.
Spegazzini C (1899). Fungi Argentini novi vel critici. Anales del Museo Nacional de
Buenos Aires, Ser. 2, 6(3): 81–365.
Spegazzini C (1923). Fungi Paraguayenses. Anales del Museo Nacional de Historia
Natural de Buenos Aires 31: 355–450.
Staleu FA, Cowan RS (1976–1988). Taxonomic literature, 2nd ed. [vols I (1976); II
(1979); III (1981); IV (1983); V (1985); VI (1986); VII (1988). Bohn, Scheltema
and Holkema, Utrecht.
Staleu FA, Mennega EA (1995). Taxonomic literature. Supplement III: Br-Ca.
Koeltz, Königstein.
Stevenson JA (1971). An account of fungus exsiccati, containing material from the
Americas. Beihefte zur Nova Hedwigia 36: 1–563.
Stevenson JA (1975). The fungi of Puerto Rico and the American Virgin Islands.
Contributions of the Reed Herbarium 23: 1–742.
the genuS Cladosporium
Steyaert RL (1930). Cladosporium hemileiae n. spec. Un parasite de l’Hemileia
vastatrix Berk. et Br. Bulletin de la Société Royale de Botanique de Belgique
63(1): 46–47.
Stohr SN, Dighton J (2004). Effects of species diversity on establishment and
coexistence: A phylloplane fungal community model system. Microbial Ecology
48(3): 431–438.
Subramanian CV (1971). Hyphomycetes: an account of Indian species, except
Cercosporae. New Delhi.
Subramanian CV (1977). Revisions of hyphomycetes–I. Kavaka 5: 93–98.
Summerbell RC, Cooper E, Bunn U, Jamieson F, Gupta AK (2005). Onychomycosis:
a critical study of techniques and criteria conirming the etiologic signiicance of
nondermatophytes. Medical Mycology 43: 39–59.
Sumstine DR (1949). The Albert Commons collection of fungi in the herbarium of
the Academy of Natural Sciences in Philadelphia. Mycologia 41: 11–23. [The
A. Commons collection is now at the Claude E. Phillips Herbarium, Dover
Delaware (DOV).]
Sutton BC (1973). Hyphomycetes from Manitoba and Saskatchewan, Canada.
Mycological Papers 132: 1–143.
Swofford DL (2003). PAUP*. Phylogenetic Analysis Using Parsimony (*and Other
Methods). Version 4. Sinauer Associates, Sunderland, Massachusetts.
Sydow H, Sydow P (1920). Weitere neue Micromyceten der Philippinen-Inseln.
Annales Mycologici 18: 98–104.
Thaung MM (1974). Two new Cladosporium species from Burma. Transactions of
the British Mycological Society 63(3): 619–622.
Thümen FKAEJ von (1877). Fungi Austro-Africani. Flora 60(26): 407–413.
Thümen FKAEJ von (1880). Contributiones ad loram mycologicam lusitanicam.
Ser. II. Hedwigia 19: 132–135.
Tracy SM, Earle FS (1896). New species of fungi from Mississippi. Bulletin of the
Torrey Botanical Club 23: 205–211.
Traquair JA, Beloche RB, Jarvis WR, Baker KW (1984). Hyperparasitism of Puccinia
violae by Cladosporium uredinicola. Canadian Journal of Botany 62(1): 181–
184.
Traverso GB (1905). Secundo contributo allo studio della lora micologica della
provincial di Como. Malpighia 19: 129–152.
Triebel D, Scholz P (2001–2011). “IndExs – Index of Exsiccatae”. Botanische
Staatssammlung München: http://indexs.botanischestaatssammlung.de.
München, Germany.
Unesco Science Cooperation Ofice for Latin America. 1955. Catálogo general de
colecciones micológicas latino americanas. Montevideo.
Untereiner WA (1997). Taxonomy of selected members of the ascomycete genus
Capronia with notes on anamorph-teleomorph connections. Mycologia 89:
120–131.
Untereiner WA, Naveau F (1999). Molecular systematics of the Herpotrichiellaceae
with an assessment of the phylogenetic position of Exophiala dermatitidis and
Phialophora americana. Mycologia 91: 67–83.
Untereiner WA, Gerrits van den Ende AHG, Hoog GS de (1999). Nutritional
physiology of species of Capronia. Studies in Mycology 43: 98–106.
Urtiaga R (1986). Indice de enfermedades en plantas de Venezuela y Cuba.
Unknown journal or publisher.
Valiušnaitė A (2002). Micromycetes infecting stone fruit trees. Biologija 1: 18–21.
Vanev SG, Taseva MN (1990). Novi parazitni g’bi po njakoi dekorativni rastenija v
B’lgarija. Fitologija 38: 84–88.
Verwoerd L, Dippenaar BJ (1930). Descriptions of some new species of South
African fungi and of species not previously recorded from South Africa. South
African Journal of Science 27: 326–330.
Viégas AP (1947). Alguns micetos Brasileiros. Bragantia 7(2): 1–48.
Vilgalys R, Hester M (1990). Rapid genetic identiication and mapping of
enzymatically ampliied ribosomal DNA from several Cryptococcus species.
Journal of Bacteriology 172: 4238–4246.
Vittal BPR, Dorai M (1994/95). Studies on litter fungi VIII. Quantitative studies of the
mycolora colonizing Eucalyptus tereticornis sm. litter. Kavaka 22/23: 35–41.
Voglino P (1913). Sopra una nuova infezione dei pomidoro. Annali della Reale
Accademia di Agricoltura di Torino 55: 379–381 “1912”.
Vries GA de (1952). Contribution to the knowledge of the genus Cladosporium Link
ex Fr. CBS, Baarn.
Vries GA de (1955). Cladosporium avellaneum de Vries, a synonym of
“Hormodendrum” resinae Lindau. Antonie van Leeuwenhoek Journal of
Microbiology and Serology 21: 166–168.
Vuillemin P (1931). Les champignons parasites et les mycoses de l’homme.
Encyclopédie Mycologique II. Paris.
Wakker JH (1896). De Schimmels in de Wortels van het Suikerried. Mededeelingen
van het Proefstation Oost-Java, Nieuwe Series, 28: 1–9.
Wang CJK, Zabel RA (1990). Identiication manual for fungi from utility poles in the
eastern United States. ATCC, Rockville, MD.
Weber FJ, Hage KC, Bont JA de (1995). Growth of the fungus Cladosporium
sphaerospermum with toluene as the sole carbon and energy source. Applied
and Environmental Microbiology 61: 3562–3566.
www.studiesinmycology.org
Webster J, Weber R (2007). Introduction to Fungi. 3rd ed. Cambridge University
Press.
White TJ, Bruns T, Lee S, Taylor J (1990). Ampliication and direct sequencing of
fungal ribosomal RNA genes for phylogenetics. In: PCR Protocols: a guide to
methods and applications (Innis MA, Gelfand DH, Sninsky JJ, White TJ, eds).
Academic Press, San Diego, California: 315–322.
Williams TH, Liu PSW (1976). A host list of plant diseases in Sabah, Malaysia.
Phytopathological Papers 19: 1–67.
Winstead NN, Strider DL, Person LH (1960). Vegetable diseases in North Carolina
during 1958–1959. Plant Disease Reporter 44: 491–495.
Wirsel SGR, Runge-Froböse C, Ahrén DG, Kemen E, Oliver RP, Mendgen
KW (2002). Four or more species of Cladosporium sympatrically colonize
Phragmites australis. Fungal Genetics and Biology 35: 99–113.
Wu DX, Zhang ZY (2003) Cladosporium tetrapanacis sp. nov. on Tetrapanax.
Mycosystema 22: 48–49.
Yamamoto W (1959). Some species of Cladosporium from Japan. Science Reports
of the Hyogo University of Agriculture, Series, Agriculture 4(1): 1–6.
Yen JM (1980). Étude sur les champignons parasites du Sud-Est asiatique. 35.
Champignons parasites de Hong Kong, I. Bulletin Trimestriel de la Société
Mycologique de France 95(3): 185–191.
Yen JM (1981). Étude sur les champignons parasites du Sud-Est asiatique. 42.
Champignons parasites de Malaisie, 21. Bulletin Trimestriel de la Société
Mycologique de France 97(3): 129–133.
Yendo Y (1919). Two new pathogenic fungi on Mulberry. Dai-Nihon Sanshi Kaiho
335: 5–6.
Yendo Y (1927). Mulberry Pathology. Meibundo, Tokyo.
Yokoyama T, Nasu H (2000). Materials for the fungus lora of Japan (54). Stenella
persicae, a new species from peach. Mycoscience 41(1): 91–91.
Yurlova NA, Hoog GS de, Gerrits van den Ende AHG (1999). Taxonomy of
Aureobasidium and allied genera. Studies in Mycology 43: 63–69.
Zalar P, Hoog GS de, Schroers H-J, Crous PW, Groenewald JZ, Gunde-Cimerman
N (2007). Phylogeny and ecology of the ubiquitous saprobe Cladosporium
sphaerospermum, with descriptions of seven new species from hypersaline
environments. Studies in Mycology 58: 157–183.
Zhang H, Zhang ZY (1998a). Taxonomy of the genus Cladosporium in China XIII.
Two new species. Mycosystema 17(4): 304–306.
Zhang T, Zhang ZY (1998b). Two new species of the genus Cladosporium. Plant
Diseases and Their Control: 108–111.
Zhang T, Zhang ZY, Liu YL (1998a). Taxonomy of Cladosporium in China XIX. A new
species and three new records for China. Proceedings of Phytopathological
Symposium Organized by Phytopathology Laboratory of Yunnan Province 2:
285–290.
Zhang Y, Crous PW, Schoch CL, Bahkali AH, Guo LD, Hyde KD (2011). A molecular,
morphological and ecological re-appraisal of Venturiales – a new order of
Dothideomycetes. Fungal Diversity 51: 249–277.
Zhang ZY, Li TF, Zhang T, Wang G (1999a). Taxonomy of the genus Cladosporium
in China XXIV. C. hydrangeae sp. nov. and two new records. Journal of Anhui
Agricultural University 26: 40–43.
Zhang ZY, Liu YL (2000). Taxonomy of the genus Cladosporium in China XXVI. C.
lathyri sp. nov. Journal of the Yunnan Agricultural University 15(3): 219–221.
Zhang ZY, Liu YL, Wei Q, He YH (1998b). Three new pathogenic fungi of
Cladosporium from China. Plant Diseases and Their Control: 103–107.
Zhang ZY, Liu YL, Zhang T, Li, TF, Wang G, Zhang H, He YH, Peng HH (2003). Flora
Fungorum Sinicorum, Vol. 14, Cladosporium, Fusicladium, Pyricularia. Beijing.
Zhang ZY, Peng HH, Liu YL, Zhang H (1998c). Taxonomy of Cladosporium in China
VII. Mycosystema 17(1): 4–6.
Zhang ZY, Wang YX, Liu YL, Li H (2000a). Taxonomy of Cladosporium in China XXI.
A new species and two new records. Mycosystema 19(2): 165–168.
Zhang ZY, Wei Q, Zhang T (1998d). Taxonomy of Cladosporium in China VIII.
Mycosystema 17(3): 195–198.
Zhang ZY, Zhang H, Li TF (2000b). Taxonomy of the genus Cladosporium in China
XXII. C. platycodonis sp. nov. and two new records. Mycosystema 19(3):
308–311.
Zhang ZY, Zhang T, Liu YL, He YH (1999b). Taxonomy of the genus Cladosporium
in China XXIII. C. forsythiae sp. nov. & two new records. Journal of Anhui
Agricultural University 26: 36–39.
Zhang ZY, Zhang T, Pu WQ (1998e). Taxonomy of Cladosporium in China VIII.
Mycosystema 17(3): 195–198.
Zhurbenko MP (2012): Lichenicolous fungi growing on Thamnolia, mainly from the
Holarctic, with a worldwide key to the known species. The Lichenologist 44:
147–177.
Zukal H (1887). Über einige neue Ascomyceten. Verhandlungen der KaiserlichKöniglichen Zoologisch-Botanischen Gesellschaft in Wien 37: 39–46.
379
BenSch et al.
INDEX OF FUNGAL NAMES
Arranged according to epithets and then genera. Synonymised
names are italicized and page numbers of main entries for true
Cladosporium species are bolded. A superscript asterisk (*) points
to pages with illustrations, a superscript c (c) to pages with a
cladogram, a superscript t (t) to pages with a table and a superscript
k (k) to pages with a key to genera or species.
The following abbreviations are used for more frequently occurring
genera.
Abbreviations: A. = Anungitopsis, Al. = Alternaria, Amphi. =
Amphitrichum, Asp. = Asperisporium, C. = Cladosporium,
Csp. = Cercospora, Csporid. = Cercosporidium, Cladophial. =
Cladophialophora, D. = Davidiella, Dem. = Dematium, Dendr.
= Dendryphiella, Didym. = Didymellina, Ex. = Exosporium, F. =
Fusicladium, Fusiclad. = Fusicladosporium, H. = Heterosporium,
Helm. = Helminthosporium, Horm. = Hormodendrum, M. =
Mycosphaerella, Megaclad. = Megacladosporium, Met. =
Metulocladosporiella, Mycovel. = Mycovellosiella, N. = Napicladium,
O. = Oidium, P. = Pseudocercospora, Pass. = Passalora, Pen. =
Penidiella, Phaeo. = Phaeoramularia, Pleosp. = Pleospora, Poll.
= Pollaccia, R. = Ramularia, Scol. = Scolicotrichum, Spadic. =
Spadicoides, St. = Stenella, Stig. = Stigmina, T. = Torula, Trim. =
Trimmatostroma, V. = Venturia, X. = Xylohypha, Z. = Zasmidium.
A
abietina, Trentepohlia 2, 296
abietinum, C. Dem. Sporotrichum 2, 296
abietinum, C. Spondylocladium 2, 296
abroniae, H. 155
acaciae, C. 72, 73, 296
acaciicola, C. 296, 354
acalyphae, C. 14, 16c, 18t, 27k, 38k, 45−46*, 119, 288
acerina, V. 317
acerinum, C. 339
acicola, Septonema 329*
Acroconidiella 11
Acrosporella 4, 11
acutum, C. 126, 155, 157
acuum, Helm. 155
adeniae, Csp. H. St. Z. 1, 336
adianticola, C. 296
aecidiicola, C. 42k, 44k, 47−48*, 114, 139, 276, 291
aequatoriense, C. Parastenella , 296*
aeruginosum, C. 339
agerati, Csp. R. Ragnhildiana 334, 335
aggregata, Pen. 10c
agoseridis, C. 31k, 37k, 49*, 86, 169, 195, 198, 224, 236
agyrioides, Epicoccum 361
ajelloi, Cladophial. 305
alba, Neofabraea 9c
albicans, C. O. Candida 296
albiziae, C. 339
albiziae, H. Helm. Camptomeris 336
album, C. Hyalodendron R. 296
algarum, C. H. 181
algarvense, Polyscytalum 9c
algeriense, C. Horm. 297
alhagi, H. 368
380
allicinum, C. D. Sphaerella Sphaeria 1, 10c, 12, 16c, 18t, 25k,
50−52*, 74, 108, 147, 260
allii, C. H. , 16c, 17, 18t, 31k, 36k, 52−54*, 55, 70, 288, 339
allii var. allii-porri, H. 53
allii var. allii-sativi, H. 53
allii var. bomareae, H. 336
allii var. cepivorum, H. 54
allii var. funkiae, H. 368
allii var. minutum, H. 368
allii var. polygonati, H. 368
allii var. sisyrinchii, H. 368
allii-porri, C. H. var. 53
allii-sativi, H. var. 53
allii-cepae, C. D. H. M. 17, 18t, 31k, 36k, 54−55*, 70, 339
alliicola, C. 288, 289*
allii-porri, C. H. var. 53
alliorum, C. 339
alneum, C. 35k, 37k, 55−56*, 60, 196
alnicola, C. Didymotrichum 56, 153, 157, 183
alopecuri, C. F. 31k, 41k, 57*, 86
alpiniae, C. 42k, 339
alstroemeriae, Asp. Scol. 336
alta, Taeniolella 9c
Alternaria 11, 67, 102, 187, 311, 314, 317, 321, 349, 368, 369, 370
alternata, Al. 352, 369
alternicoloratum, C. 297
amaranticola, C. 154
ambrosiae, C. 340
americana, Devriesia 5*
americana, Poll. 318
americanum, C. 297, 304, 305
amoenum, A. C. F. 10c, 297
amorphae, C. 297
Amorphotheca 24k
ampelinum, C. 297, 335
Amphiconium 300
amphitrichum, C. 340
amsoniae, H. 183
amygdali, F. 304
angelicae, Diaporthe 9c
angelicae, M. 309
angustisporum, C. 12, 16c, 18t, 29k, 41k, 57−58*, 265
Annellosympodia 136
annonae, C. 35k −36k, 59−60*
anomalum, C. P. 297
antarcticum, C. 12, 16c, 18t, 27k, 42k −43k, 60−62*, 89, 147, 190
antillanum, C. , 297
Anungitea 22k, 24k −25k
aphidis, C. 16c, 18t, 34k, 44k, 62−64*
aphidis var. muscae, C. 340
apicale, C. 13, 33k, 38k, 64−65*, 102, 130, 131, 200, 277, 356
apiculatum, C. 340
apii, Csp. 169, 316, 365
Apiosporina 335
aplospora, R. 10c
aquilinum, C. 340
arachidis, Csp. Septogloeum 326
araguatum, C. St. 4, 5*, 10c, 297, 305
araliae, C. St. Z. 297−298*
arcticum, C. Pleosp. 340
arcuata, Periconiella 10c
arenarium, Scolecobasidium 337
the genuS Cladosporium
argillaceum, C. Rhizocladosporium 6*, 9c, 297
aristolochiae, C. 37, 340
aromaticum, C. F. 298*, 323
artemisiae, C. F. 298*
arthoniae, C. 62, 298
arthrinioides, C. 14, 34k, 40k, 66−67*
arthropodii, C. 16c, 18t, 31k, 37k, 67−70*
articulatum, Dem. 307, 346
artocarpi, C. 203
arundinaceum, C. 298, 340
arundinaceum, Helm. N. Deightoniella 326
arundinicola, C. 340
arundinis, C. Myxocladium 153, 154
asiaticum, F. 321
asperatum, H. 155
aspericoccum, C. 192, 193
asperistipitatum, C. 33k, 41k, 70*
aspericoccum, C. 192, 193
asperulatum, C. 16c, 18t, 28k, 70−72*, 93
asperum, Trichocladium 336
astericola, C. F. 298, 299*
asterinae, C. Parapericoniella 4, 8*, 298, 299*, 300
asteroma, C. N. 223, 299, 329
asteroma var. macrosporum, C. 299
asteroma var. microsporum, C. 299
asteromatoides, C. 340, 341*
astroideum, C. var. 26, 72−73*, 261
astroideum var. catalinense, C. 1, 27, 73*
aterrimum, C. 299
atopomerum, H. 155
atra, Steptothrix 327
atriellum, C. 203
atriplicis, C. 341
atrofumosa, Botrytis Virgaria 319
atroseptum, C. 341
atrovirens, Spondylocladium 296
atrum, C. 2, 300
aurea, Trentepohlia 2, 300
Aureobasidium 307, 334
aureum, C. 2, 300
auriculae, C. H. 27k, 35k, 41k, 73−74*
australiense, C. 12, 16c, 18t, 30k, 74−75*, 211
australiensis, Cladophial. 9c
autumnale, C. 341
avellaneum, C., f. 4, 24k, 300, 329
avellaneum f. albidum, C. 300, 329
avellaneum f. sterile, C. 300, 329
avellaneum f. viride, C. 300, 329
avenae, H. 155
avenaria, Phaeosphaeria 9c
azaleae, Seifertia 9c
Azosma 11
B
baccae, C. 341
bacilligerum, C. Pass. Scol. 56, 300
balladynae, C. 300, 301*
banaticum, C. 341
bantianum, C. Cladophial. T. X. 300, 333
baptisiae, C. F. 300, 301*
barretoana, Pass. 302*, 326
www.studiesinmycology.org
basiinlatum, C. 16c, 18t, 28k, 75−77*, 238
beckii, H. 368
beijerinckii, C. Coryneum 301
bellynckii, C. Csp. Csporid. Mycovel. Pass. 301
berberidis, H. 155
berkeleyi, M. 326
berkeleyi, Polyscytalum 315
berkheyae, C. Fulvia Mycovel. Pass. 301
betae, H. 368
beticola, Csp. 16c
betulae, Hyalodendriella 7*, 9c
betuligenum, C. F. 301, 302*
binum, Helm. Scol. Spadic. 299
bignoniae, C. 158, 341
Bispora 22k
bisporum, C. Beejadwaya 301−302
Bistratosporium 11, 98
boenninghauseniae, C. 342
borassi, C. 31k, 36k, 77*, 85
borealis, Poll. 320
bosciae, C. 35k, 38k, 77−79*, 107−108, 176
Botrytis 185
brachormium, C. 342
brachycarpa, Csp. Mycovel. Pass. 331
brachyelytri, C. 302
brachytrichum, C. Didymotrichum 302
brassicae, C. Cladotrichum H. 31k, 38k, 79*−80, 342
brassicicola, C. 79, 342
brevicatenulatum, C. 342
brevicompactum, C. 342
brevicompactum var. tabacinum, C. 342
brevipes, C. 302, 318, 332
breviramosum, C. 302
britannicum, C. F. 303*
bruhnei, C. Horm. 25k, 50, 52, 74
brunneoatrum, C. 98, 303
brunneolum, C. 155
brunneum, C. 155, 157, 181, 184, 185, 205, 223
bryoniae, Didymella 9c
burtonii Endomycopsis Hyphopichia Pichia 311
buteicola, C. 34k, 38k, 80*, 347
butyri, C. 303
C
caducum, C. F. 303*
caesalpiniae, C. F. 303, 304*
caespiticium, C. 154, 157
caespitosa, Byssus 303
?caespitosum, C. 345
calamigenum, C. 303
calandriniae, H. 183
calcareum, C. 342
californica, Pass. 7*
californicum, H. 336
callae, C. Csp. 304*
callospermum, H. 336
calotropidis, C. Csp. Cercosporina N. Pass. Phaeo. 304
cancerogenes, C. 304
candida, Albugo 261
Capronia 3
capsici, C. Csp. 304
381
BenSch et al.
capsicicola, Csp. Pass. Phaeo. 304
caraganae, C. 34k, 36k, 39k, 80−81*
caraganae, H. 368
cardariae, C. 181
caricicola, C. Didymotrichum 153, 157
caricinum, C. 38k, 342
caricis, H. 155
carispermum, Helm. 307
carpesii, C. 35k, 37k, 81
carpineum, F. 305
carpophila, Stig. 301
carpophilum, C. F. Fusiclad. Megaclad. V. 10c, 297, 304, 305*
carrionii, C. Cladophial. 305
caryigenum, C. F. 305, 309
caryigenum var. carpineum, C. 305
casei, C. D. 305
cassiae-surathensis, C. 32k, 35k, 81−83*, 175
cassiicola, Csp. 326
cassiicola, R. 326
Castanedaea 23k
castellanii, C. 297, 305
castellanii, Exophiala 321
catamarcense, C. 305
cattleyae, C. 198, 305
caulicola, H. 155
cboliae, Rachicladosporium 10c
celastrinum, H. 368
cellare, C. Racodium Rhinocladiella Z. 4, 5*, 10c, 305
cellaris, Antennaria 305
centaureae, H. 368
cerasi, C. Acrosporium F. Fusicladiopsis Megaclad. Karakulinia
V. 305−306
cerastis, H. 183
cercestidis, C. St. Z. 306
Cercospora 86, 176, 365
cercosporoides, H. 368
cerevisiae, Saccharomyces 9c
cerophilum, C. Acrotheca Ramichloridium 306
cetera, Al. 321
chaetomium, C. Csporid. Pass. 306
chaetospira, Cladophial. 9c, 24k
Chalastospora 85, 321
chalastosporoides, C. 16c, 18t, 29k, 43k, 83−85, 160
chamaeropis, C. 33k, 36k, 85−86*, 175, 178, 295
chamaeropis, H. 183
cheonis, C. Csp. 33k, 36k, 85−86*, 178
chionanthi, Pass. 313
chlamydeum, C. 306
chlamydospora, C. Devriesia 306
chloridis, H. Acroconidiellina 336
chlorocephalum,
C.
Dichocladosporium
Graphiopsis
Haplographium Periconia 4, 8*, 306*, 307, 324
chlorocephalum var. ovalisporum, Haplographium 307
chodatii, C. Dem. Candida 307
chromoblastomycosa, Catenulostroma 10c
chrysanthemi, C. 342, 343
chrysophylli, C. 12, 31k, 41k, 87*
chubutense, C. 16c, 18t, 28k, 40k, 88−89*, 97, 220
cinerea, Botrytis 101
cinnamomeum, C. Scol. 199, 307
cinnamomi, C. St. Scol. Z. 307
circaeae, C. 40k, 343
382
circinale, H. 111, 112
circinalis, C. 307
citri, C. Kurosawaia 98, 307, 343, 346
citri, Z. 10c
citrina, Bisporella 9c
citricola, Diplodia 98, 364
Cladophialophora 3, 22k, 24k, 25k, 293, 322
cladosporioides, C. Horm. Penicillium 2−3, 10c, 11−15, 16c,
17, 18t, 22, 26k, 27, 29k, 35k, 58, 74−75, 77, 79, 81, 90−93*,
101−102, 106, 119−121, 124, 129−130, 133, 136, 140−142,
161, 163, 165−166, 172−173, 177, 188, 196, 206, 208, 210,
221, 223, 227−229, 232−233, 243−245, 253, 264−265, 269,
272, 276, 283, 290, 293−294, 332, 339, 345, 347−350, 352,
357, 360, 367
cladosporioides, H. 155
cladosporioides f. sp. pisicola, C. 221
cladosporioideum, Scol. 166
cladosporiosa, Sphaeria 313
Cladosporium 1−4, 9−15, 17, 18, 20−21, 22k, 25, 30, 36, 42,
44−46, 49, 52, 56−57, 60, 62, 79, 81, 85−87, 93, 98, 107−108,
116, 126−127, 129, 131, 134−136, 144, 157, 160, 162−163,
165−166, 169, 175−176, 178, 185, 187, 192, 195−196,
198−200, 206, 221−224, 229, 232−233, 235−236, 239,
241, 247, 250, 253−254, 256−257, 264, 267, 269, 272, 274,
288, 293, 296−298, 300, 302−303, 305, 307−315, 317−319,
321−323, 325, 327, 330−332, 335−339, 341, 343, 346−348,
350−356, 358, 360−362, 364−369
cladrastidis, C. Csp. P. 307
clappieri, C. 307
Clasterosporium 298
clavatum, C. 343
clavisporium, Graphium Isariopsis 335
clemensiae, C. 343
cleomis, H. 182
coccolobae, Parapleurotheciopsis 24k
coelosporum, C. 307
Colletotrichum 310, 311, 315
collinsii, Apiosporina 335
colocasiae, C. 12, 16c, 18t, 28k, 32k, 36k, 93−95*, 133
colocasiae, H. 336
colocasiae, Johnstonia 336
colocasiicola, C. 93, 95
colombiae, C. 16c, 18t, 28k, 95−97*
columbiana, Pen. 6*, 8*
comesii, C. 343
compactiusculum, C. 343, 364
compactum, C. Csporid. Pass. 307, 343, 364
compactum, Helm. 155
compactum f. bosciae, C. 77
compactum var. bosciae, C. 77
compactum *punctatum, C. 343, 361
condylonema, C. 155, 157
confusum, C. 307
congestum, C. 199, 307, 331
coniothecii-gonorrhoici, C. 314
coniothecii-syphilitici, C. 332
Coniothyrium 356
consimile, Septoidium 334
conspersum, Haplotrichum 9c
constrictum, Ochroconis Scolecobasidium 338
convolvularum, F. 10c
coorgica, Cercosporella 335
the genuS Cladosporium
coralloides, C. 129, 308
corchori, C. 39k, 344
coreopsidis, C. F. 308*
cornigenum, C. 308
corrugatum, C. 32k, 41k, 97*−98
coryneoidea, Strumella 327
coryneoides, Csp. 330
corynitrichum, C. 344
coryphae, C. H. 11, 30k, 36k, 85, 98*, 107−108
cryptarum, Byssus 305
cubense, C. Pen. 308, 318
cubisporum, C. Coremiella Briosia 308
cucumerina, Al. 101
cucumerinum, C. 16c, 18t, 29k, 35k, 38k, 98−101*, 228, 290
cucumerinum, Macrosporium 101
cucumerinum var. europaeum, C. 99, 101
cucumeris, C. 99, 101
cucurbitacearum, Didymella 9c
cumulus, C. 308
curtisii, Haplotrichum 9c
Curvularia 368
cycadacearum, C. 64, 65
cycadis, C. 32k, 38k, 65, 101*−102
cyclaminis, C. 308
cyclaminicola, R. 308
cynarae, R. 6*
cyrtomii, C. 38k, 344
cytisi, H. 155
cyttariicola, C. 308
D
dahliae, Pass. 10c
dalmaticum, H. 336
daphniphylli, C. 308
Davidiella 1−4, 10, 11−12, 13, 15, 22, 52
dearnessianum, F. Acrotheca 327
decipiens, Athelia 9c
decolorans, C. 308
delexum, Myxotrichum 9c
deightoniana, St. Z. 306
delectum, C. 26k, 32k, 39k, 102*
delectum f. ailanthi-glandulosae, C. 344
delicatulum, C. 16c, 18t, 26k, 30k, 46k, 80, 102−106*, 160, 165, 193,
241
dematiosum, C. 308
dendriticum, C. F. Pass. 308, 309, 328
dendriticum var. orbiculatum, F. Pass. 324
dendriticum var. beta orbiculatum, C. 309
dendriticum var. heteromeles, C. 309
dendryphioides, C. 309
densum, C. 344
densum, Trichosporium 327
depressum, C. Csp. Csporid. F. Megaclad. Pass. Scol. 125, 309
depressa f. angelicae, Csp. 309
desmodicola, C. 344
desmotrichum, C. 126, 157, 344
destruens, F. 182
deusta f. odorati, R. 297
deusta var. alba, R. 297
Devriesia 4, 24k
devriesii, C. Cladophial. 309
www.studiesinmycology.org
dianellae, Csp. H. St. Z. 1, 336, 345
dianellicola, C. 42k, 344
dianthi, H. D. M. 111, 112
diaphanum, C. 12, 32k, 41k, 106−108*, 130, 180, 347
Dichocladosporium 4
didymosporum, H. 155
Didymotrichum 4
dieffenbachiae, C. Septoria 25k, 31k, 36k, 108*
digitalicola, C. 42k, 187, 345
Digitopodium 4, 23k
diospyros, F. 318
dirinae, Verrucocladosporium 7*, 10c
dissiliens, T. Pass. Phaeo. Septocylindrium 326, 330
domingensis, Csp. 304
dominicanum, C. 16c, 18t, 26k, 108−110*,
Drechslera 169
dracaenatum, C. 33k, 37k, 110−111*, 125, 149, 200
dufourii, C. 345
E
echinulatum, C. H. Helm. 16c, 18t, 31k, 38k, 111−114*
echinulatum var. dianthi, H. 111
edgeworthiae, C. 42k, 345
effusum, C. Csp. Didymaria Pass. 309, 325
effusum, C. F. Fusiclad. 10c, 305, 309
effusum var. carpineum, F. 305
elaeagnus, C. 309, 345
elatum, C. Cadophora Horm. Ochrocladosporium 6*, 9c, 302, 309
elatum, Glyphium 9c
elegans, C. 155, 157, 309−310
elegans var. singaporense, C. 98, 310
elegantulum, C. 345
ellipsoidea, Pen. 10c
ellipsospora, Periconia 307
ellisii, Rhinocladiella 305
elmeri, H. 368
elsinoes, C. 310
emmonsii, Cladophial. X. 300
endophylla, R. 10c
entoxylinum, C. 193, 310, 362
epacridis, C. 310
ephedrae, H. 155
epibryum, C. 345
epichloes, C. 42k, 43k, 48 k, 114, 180*
epimyces, C. 43k, 44k, 114−115*, 180
epimyces, H. 155, 157
epiphylla, Athelia 9c
epiphyllum, C. Dem. Chloridium 153, 339
epiphyllum (beta) chionanthi, C. Dem. 153, 157
epiphyllum f. castaneae-sativae, C. 345
epiphyllum var. acerinum, C. 183, 185
episclerotiale, C. 11, 43k −44k, 115−116*, 138, 157, 180, 257
episphaerium, C. Dem. 310
epixilinum, C. 310
equiseti, C. 183−184
equiseti, H. 155
eremostachydis, H. 368
erianthi, C. 310
eriobotryae, C. F. 33k, 41k, 116−117*, 130
eriodictyonis, Trim. Coniothecium 336
eriolobi, C. St. Z. 310*
383
BenSch et al.
erysiphoides f. cordiae, O. 327
eschscholtziae, C. H. Acroconidiella 310, 336
eucalypti, Aulographina 10c
eucalypti, Blastacervulus 10c
eucalypti, C. 345
eucalypti, Cladoriella 10c
eucalypti, H. 368
eucalypti, Heteroconium 10c
eucalypti, Phlogicylindrium 9c
eucalypti, Torrendiella 9c
eucalypti var. maculicola, H. 336
eucalypticola, C. 310
euonymicola, Erysiphe 253
euphorbiae, C. 345
euphorbiae, Scol. Piricularia 306
exasperatum, C. 14, 16c, 18t, 28k, 40k, 117−119*, 288
exasperatum, H. Helm. 111
exile, C. 16c, 18t, 27k −30k, 119−121*, 124, 210, 215, 276
exoasci, C. 215, 217−218, 267, 349
exobasidii, C. var. 42k, 44k, 120−122*
exobasidii var. verruculosum 42k, 44k, 121−122*
Exophiala 334
extoma, C. 311
extorre, C. 311
F
fagi, C. 155
fagi, F. 193
farnetianum, C. 98, 307, 346
fasciculare, C. 307, 346, 352
fasciculare, Helm. Septonema 329
fasciculare f. asparagi-oficinalis, C. 311
fasciculare f. chamaeropis, C. 85
fasciculatum, C. 153, 355
fasciculatum f. amerotrichum, C. 346
fasciculatum f. gladioli, C. 50
fasciculatum f. scirpi-lacustris, C. 102, 106
fasciculatum var. densum, C 102, 106, 346
fawcettii, Sphaceloma 343
fecundissimum, Polyscytalum 9c
fenestrale, Byssocladium 352
fermentans, C. 311
ferox, C. H. 30k, 41k, 122−123*
ferrugineum, C. 199, 311
festucae, C. 346
ici, C. 346
labelliforme, C. 16c, 18t, 29k, 40k, 123−124*
lexuosum, Helm. Brachysporium 153
lueggeae, C. 311
foliorum, C. 12, 33k, 36k, 124−125*, 247
foliorum, Syncollesia 312
forsythiae, C. 40k, 346
foveolicola, C. Pass. 311*−312
fraxini, H. 155
fraxinicola, C. 36k, 40k, 125*−127*
frigidarii, Ochrocladosporium 9 c
fuckelii, Csp. Isariopsis Septosporium 330
fuligineum, C. 116, 121, 154, 157, 180
fuligineum f. racemosum, C. 312
fuliginosum, Sporotrichum 319
fuligo, C. 347
384
fulvum, C. Fulvia Mycovel. Pass. 312, 347, 354
fulvum var. violaceum, C. 312, 347
fumagineum, C. 35k, 39k, 127*−128*, 311
Fumago 312, 326, 334, 368, 369, 370
fumago, C. T. Caldariomyces Leptoxyphium 312, 313, 334
fumago, C. Hyphosoma N. 313, 334
fumago f. artemisiae abrotani, C. 312
fumago f. carpini betuli, C. 312
fumago f. corticicola, C. 312, 313
fumago f. coryli, C. 312
fumago f. fragariae-vescae, C. 312
fumago f. fraxini, C. 126, 312
fumago f. grossulariae, C. 312
fumago f. humuli-lupuli, C. 312
fumago f. poae-pratensis, C. 312
fumago f. quercus, C. 312
fumago f. rosae-acutifoliae, C. 312
fumago f. rosae-albae, C. 312
fumago f. syringae-vulgaris, C. 267, 313
fumago f. ulmi, C. 313
fumago f. ulmi-effusae, C. 313
fumago f. vitis, C. 313
fumago var. betulae, C. 313
fumago var. corticola, C. 313
fumago var. elongatum, C. 313
fumago var. epiphyllum, C. 313
fumago var. maculaeforme, C. 267, 313
fumago var. padi, C. 313
fumago var. rubi, C. 313
fumosa, C. 347
fungicola, H. 182
fungorum, C. 153, 313
funiculosum, C. 16c, 18t, 29k, 128*−129*, 276
furfuraceum, C. 98, 347
Fusarium 343
fuscatum, C. 153
fuscescens, F. 327
fuscum, C. Dem. 153, 347
fusicladiiformis, C. 12−14, 31k, 41k, 117, 130*−131*, 200, 266
Fusicladium 2, 3, 4, 13, 22, 24, 25, 33, 124−125, 198, 223−224,
241, 247, 293, 297, 333, 335
fusicladium, C. 155, 157
fusiforme, C. 16c, 18t, 27k, 64, 131−133*, 340
fusiforme, C. Hyalodendron Retroconis 313
fusimaculans var. barretoana, Pass. 326
fusisaprophyticus, Subramaniomyces 9c
fusisporum, C. 313
G
galegae f. lathyri, R. 296
galii, C. 32k, 41k, 131−133*, 177
galii, H. 155
gallicola, C. 43k, 134*−135*, 138, 175, 266, 366
gamsianum, C. 16c, 18t, 29k, 135*−136*
geniculatum, C. 313
gentianae, C. 12, 33−34k, 39k, 70, 77, 136−138*, 186
georginae, C. 313
germania, Catenulostroma 10c
gerwasiae, C. 43k −44k, 138*−139*
gleditschiae, C. 35k, 39k, 139*−140*
globisporum, C. 16c, 18t, 29k, 139−142*
the genuS Cladosporium
globosa, Conoplea Streptothrix 327
globosum, Chaetomium 9c
globosum, Trichoderma 327
glochidionis, C. 347
gloeosporioides, C. Dischloridium 313−314*
goiranicum, H. 155
Gonatophragmium 320
gonorrhoicum, C. Coniothecium 314
gossypii, Al. 314
gossypii, C. Chalastospora 4, 85, 314, 321, 327, 348
gossypiicola, C. 93, 347
gossypiicola var. minor, C. 348
gougerotii, C. Dem. T. Oospora Phialophora Rhinocladium
Sporotrichum 314
gracile, C. Didymotrichum 181, 185
gracile, H. 166
gracile var. muscari, H. 369
gramineum, C. 314
gramineum, H. Helm. Drechslera 337
graminis, C. 369
graminis, H. 369
graminis, Pass. 331, 337
graminis f. sp. bromi, Blumeria 9c
graminum, C. Dem. Chloridium 153, 180−181, 184−185, 369
graminum f. bambusae, C. 348
graminum f. inlorescentiae, C. 348
graminum f. poae-pratensis, C. 348
graminum var. moliniae-caeruleae, C. 155
graminum var. scirpi, C. 315
graminum var. sorghi, C. 157, 315
granulatum, H. Helm. 337
Graphiopsis 4, 23k
grech-delicatae, C. 12, 35k, 41k, 141−143*
grevilleae, C. 16c, 18t, 41k, 142−144*
grewiae, C. 348
griseo-olivaceum, C. 348
griseum, C. Dendryphion 315
griseum, Polyscytalum 315
groenlandicum, H. 369
grumosum, C. Dem. 348
guanicense, C. Csporid. Pass. Polythrincium 315
guareicola, Helm. Pleurophragmium Spiropes 98, 310
gynoxidicola, C. Pass. St. 163, 315*
H
halotolerans, C. 16c, 18t, 26k, 110, 144−146*, 229
hanliniana, V. 10c
haplophylli, C. H. 31k, 41k, 146*
Haplotrichum 24k
harknessii, C. Monilia 315
hartigi, Pestalozzia 354
hederae, C. 155, 157
heleophilum, C. 27k, 42k, 146−147*
helicosporum, C. 315
heliotropii, C. 14−15, 34k, 38k, 147−149*, 161
helminthosporioides, C. Azosma Macrosporium 11, 348
hemileiae, C. Digitopodium 4, 8*, 315−316*
henningsii, Csp. 192
herbaroides, C. 12, 16c, 18t, 25k, 149−152*
herbarum, C. Dem. Helm. Acladium Byssus 2−4, 11−15, 16c, 17,
18t, 25k, 42k, 48, 52, 56, 59, 62, 64, 74, 77, 79, 85, 91, 95,
www.studiesinmycology.org
102, 108, 114, 116, 121, 126, 127, 130, 133, 147, 152−157*,
161, 171, 175, 180, 185, 192-194, 203, 205, 222, 232, 235,
245, 250, 253, 256, 258, 262, 264, 269, 281, 291, 303−304,
307, 310, 312−313, 315, 317, 320, 326, 328, 331−332, 334,
339−340, 342−346, 348−349, 351−352, 354, 361−362, 366,
368−370
herbarum, Phoma 9 c
herbarum, T. 300
herbarum α aphidis, C. 351
herbarum ß foliorum, C. Dem. 181
herbarum ß brassicae, Dem. 180, 184
herbarum ß fasciculare, C. 352
herbarum γ fungorum, C. 153
herbarum ß nigricans, C. 352
herbarum δ cerealium f. hordei, C. 50
herbarum δ lignorum, Dem. 300
herbarum b solutum, C. 362
herbarum f. agaves-echeveriae, C. 348
herbarum f. amaranthi, C. 155
herbarum f. asparagi, C. 348
herbarum f. Bambusae arundinaceae, C. 155
herbarum f. Brassicae Botrytis, C. 155
herbarum f. camelliae-japonicae, C. 90
herbarum f. carpophilum, C. 348
herbarum f. dianthi, C. 155
herbarum f. epixylon, C. 192−193
herbarum f. imicola, C. 348
herbarum f. losculorum, C. 348
herbarum f. Foeniculi oficinalis, C. 155
herbarum f. fructicola, C. 349
herbarum f. Holci mollis, C. 154
herbarum f. hormodendroides, C. 90
herbarum f. mesembrianthemi, C. 349
herbarum f. napi, C. 154
herbarum f. parasiticum, C. 43k −44k, 349*
herbarum f. petiolorum fraxini, C. 315
herbarum f. phaseoli, C. 154
herbarum f. Phaseoli vulgaris, C. 349−350*
herbarum f. psoraleae, C. 350
herbarum f. repens, C. 351
herbarum f. rubi, C. 315, 316
herbarum f. saxicola, C. 351
herbarum f. sechii-edulis, C. 316
herbarum f. stellariae, C. 155
herbarum f. Tritici vulgaris, C. 154
herbarum var. aphidicola, C. 351
herbarum var. aphidis, C. 351
herbarum var. brassicae, C. 351
herbarum var. cellulosae, C. 351
herbarum var. cerealium, C. 351
herbarum var. citricola, C. 269
herbarum var. densum, C. 351
herbarum var. ephedrae, C. 351−352*
herbarum var. epixylinum, C. 310
herbarum var. fasciculare, C. 352
herbarum var. imicola, C. 348
herbarum var. losculorum, C. 348
herbarum var. foliorum, C. 181
herbarum var. hypharum, C. 316
herbarum var. indutum, C. 72
herbarum var. lablab, C. 352
herbarum var. macrocarpum, C. 180
385
BenSch et al.
herbarum var. macrosporum, C. 352
herbarum var. nigricans, C. 352
herbarum var. phlei, C. 352
herbarum var. rhois, C. 351
herbarum var. rubi, C. 316
herbarum var. solutum, C. 362
herbarum var. torulosum, C. 154
herbarum var. typharum, C. 154, 316, 352
herbarum var. vincetoxici, C. 155
herbarum var. vitricola, C. 352
Heteroconium 24k
heteronemum, C. Helm. Macrosporium 316
heterophragmatis, C. 34k, 37k, 67, 157−158*
Heterosporium 1−4, 11, 45, 169, 194, 238, 245, 274, 336, 352,
368−369
heterosporium, C. 316
heterosporum, Acladium 153
heuglinianum, C. 352
hibisci, C. 250, 253, 353
hillianum, C. 16c, 18t, 29k, 85, 158−160*
hippocastani, Dem. 352
homopilatum, Chaetomium 9c
hordei, C. Horm. 50
hordei, H. 183
Hormiactis 22k
Hormoconis 24k
Hormodendropsis 11, 13
Hormodendrum 13
hoveae, C. P. 316*
humicola, Monilia 90
humile, C. F. Fusiclad. 316, 317
Hyalodendriella 4, 22k−23k
Hyalodendron 22k
hybridum, H. 182
hydrangeae, C . 39k, 353
hydropiperis, Csp. Helm. 309
hypophloeum, C. 353
hypophyllum, C. 12, 34k, 42k, 160*−161*
I
idahoense, Parahaplotrichum 315
idesiae, C. 183, 184, 196
illionensis, Csp. 322
inaequalis, V. 10c, 309, 324
inaequiseptatum, C. Parapleurotheciopsis 317
inconspicua, Csp. 304
inconspicuum, C. 34k, 42, 162*
indigoferae, C. 291−293
infuscans, C. Dendr. 310, 317*
inopinum, C. Csp. 32k, 37k, 49k, 163*, 195, 224
inquinans, O. T. 327
insectorum, C. 353
intermedia, Didym. 53
interseminatum, Dendr. H. Helm. Dendryphion 337
inversicolor, C. 14, 16c, 18t, 28k −29k, 46k, 106, 163−165*
iponemensis, Csp. Cercosporina 326
iranicum, C. 16c, 19t, 30k, 144, 165*−166*, 208
iridicola, C. 353
iridis, C. H. Scol. 10c, 16c, 19t, 31k, 39k, 166−169*, 226
iridis-pumilae, H. 166
irritans, Toxicocladosporium 7*, 10c
386
iteodaphnes, Csp. Helm. 331
J
jacarandae, C. F. 317*
jacarandicola, C. 32k, 37k, 86, 158, 169*−170*, 190, 236
jaczewskii, C. 317
jaguarensis, Csp. 331
javanicum, C. 353
judaica, Csp. 330
juglandinum, C. 353
juglandis, C. 72
jujubae, Csp. P. 335
juncicola, C. Brachysporium Helm. 50, 353
K
kaki, F. 318
kapildharens, C. 353
kellermaniana, Cladophial. Phaeo. Pseudocladosporium 321
kleinziense, Heteroconium 10c
kniphoiae, C. 181, 185
L
laburni, H. 155
lacroixii, C. 31k, 36k, 169−171*
lactucae, C. 317−318
lactucicola, C. Pass. 317−318*
ladina, Leptosphaeria Nodulosphaeria 318
ladinum, C. 318
lageniforme, F. 318
lagunense, H. 337
lallemantiae, H. 369
lanciforme, C. 181
langeronii, C. Horm. 16c, 19t, 26k, 44k, 171*−172, 229
lantanae, C. 318, 333
lantanae, Csp. Mycovel. Pass. 333
lantanae, Chaetotrichum 333
lantanae var. cubensis, Mycovel. Pass. 318
lantanae var. verbenacearum, Mycovel. 333
laricis, C. 155, 157, 354
laricis, H. 369
laricis var. pini-pineae, C. 354
laricinum, H. 183
lathyri, C. 39k, 354
lathyri, R. 296−297
lauri, C. 200, 354
laxicapitulatum, C. 165, 354
laxum, C. Mycovel. Pass. 318
leguminicola, C. 27k, 33k, 39k, 172−173*, 350, 360
leoni, Csp. 330
leproides, C. Horm. Scopulariopsis 318
leprosum, C. 354
leptoderma, Aporothielavia 9c
lethiferum, C. Poll. 223, 318
lethiferum, C. F. var. 223, 318
levieri, C. F. Phaeo. Ragnhildiana 318
lichenicola, C. 62, 175, 318
licheniphilum, C. 16c, 19t, 29k, 43k, 62, 173−175*, 217
lichenopsis, T. 250, 253
lichenum, C. P. 62, 175, 319
the genuS Cladosporium
lignatile, C. Virgaria 319
lignicola, C. 153, 250, 253
ligustri, M. Septoria 161
lilacis, Csp. Ex. H. P. 337
lineolatum, C. 14, 32k, 35k, 38k, 175*−176*, 188
lingelsheimii, Csp. 304
linicola, C. 355
liriodendri, C. 35k, 39k, 176*−177*
lobeliae, H. 369
lobeliae-cardinalis, Pass. 325
longicatenata, Anungitea 24k
longipes, C. 355
lonicerae, C. 319, 320
lonicerae, H. 369
lonicericola, C. St. Z. 319*
lophodermii, C. 355
luci, H. 369
luculiae, Rachicladosporium 5*, 10c
lupiniphilum, C. 13, 32k, 39k, 169, 177−178*, 198, 236
lychnidis, C. 38k, 355
lycoperdinum, C. 16c, 19t, 29k, 43k −44k, 77, 108, 178*−180
lycopersici, C. 355
Lylea 22k
lysimachiae, C. F. 320*
lythri, C. Csp. St. Z. 1, 320
M
machili, C. 199, 320
macrocarpa, D. 183, 185
macrocarpum, C. 12, 16c, 19t, 25k, 31k −32k, 42k, 52, 54, 89−90,
133, 147, 169, 170, 180*−185, 194, 196, 281, 291, 312, 339,
340, 352, 353, 361, 368, 369, 370
macrocarpum f. fraxini, C. 155
macrospora, D. M. Didym. 144, 166
Macrosporium 11
maculans, C. Helm. 355, 359
maculatum, C. 320
maculatum, H. 182
maculicola, C. Phaeo. T. 223, 320, 332
madagascarense, C. Horm. 320, 359
magnoliae, C. 183−184
magnoliae, H. Parastenella Stenellopsis 337
magnusianum, C. H. 201, 203
malopis, C. 320
malorum, C. Al. 4, 314, 320-321, 327
malvacearum, C. 355−356
manoutchehrii, C. 356
mansonii, C. Dem. T. Aureobasidium Exophiala Foxia Malassezia
Microsporum
Pullularia
Rhinocladiella
Sporotrichum
Wangiella 321
maracuja, C. 32k, 40k, 85-86, 185*−186*
marinum, C. 321
marmorata, Csp. Phaeo. Pass. Cercosporina 330
martianofianum, C. F. 223, 321
maydis, H. 183
medicaginis, H. 369
melanophlaei, C. 356
meliloti, R. 90
melophthorum, Scol. Macrosporium 99
melospermae, C. 30k, 40k, 186*−187
menispermi, C. 183, 184
www.studiesinmycology.org
metaniger, C. Cryptococcus 321, 335
metaplexis, C. 36k, 356
Metulocladosporiella 4, 23k, 129, 296
microporum, C. 154, 157, 192, 356
microspermum, C. 356
microsora, Csp. 304
microspilum, C. 356
microsporum, C. 356
microstictum, C. 160, 161
mikaniae, C. Mycovel. Pass. 321
milii, C. Pass. 321
mimulicola, C. 34k, 40k, 187*
minor, C. 321, 322
minor, C. Castanedaea 321, 322
minourae, C. Cladophial. 322
minus, Alysidium 321, 322
minus, C. 321, 322
minusculum, C. 12, 14, 33k, 41k, 187−188*, 341
minutulum, Asp. 328
minutulum, H. 181
miyakei, C. 357, 359
modestum, C. Denticularia 322
moldavicum, C. 155, 157
molle, C. Csp. 322
mollissima, Byssus 305
monardae, C. F. 322*
montenegrinum, H. 166
mori, C. Horm. 357
multigeniculatum, C. 92, 357
munduleae, H. Sirosporium 337
murorum, C. 322
musae, C. Met. 4, 7*, 9c, 322*−323
musae, Periconiella 323
musae, Chaetophoma 206
musicola, Met. 9c
Mycosphaerella 2−4, 12, 17, 22, 24
Mycovelosiella 24k
Mydonosporium 11
myriosporum, C. 90, 183
myrmecophilum, C. Cladotrichum Macrosporium Septosporium 357
myrtacearum, C. 16c, 19t, 28k −29k, 32k, 40k, 71, 85, 126, 144, 175,
188−190*, 196
myrticola, C. F. 323*
Myxocladium 4
N
nanum, Helm. Dendryphion 321−322
nanum f. petiolicola, Helm. 155
nectandrae, Pen. 311
neocheiropteridis, C. 41k, 358
Neofusicoccum 92
neottopteridis, C. 37k, 358
nerii, C. 192
neriicola, C. 34k, 36k, 190−192*
nervale, C. 298, 323
nervisequum, C. 130, 358
neuhofii, Athelia 9c
nicotianae, C. 358, 364
nigra, Botrytis Sporotrichum Trichosporum Virgaria 319
nigra, Monilia 303
nigrelloides, C. 32k, 38k, 192*
387
BenSch et al.
nigrellum, C. 26k, 192−193*, 241
nigricans, Byssus 352
nitrariae, C. 358
nodulosum, C. Didymotrichum 153
nopomingensis, Mycovel. Pass. 333*
O
oblongum, C. 25k, 30k, 37k, 194*
obtectum, C. 17, 34k, 37k −38k, 194*−195*, 261, 323
occidentalis, Csp. Pass. Phaeo. 326
occidentalis, R. 326
occultum, C. Dactylosporium 323
Ochrocladosporium 4, 25 k
Ochroconis 338
Oidium 369
oleacina, Chaetophoma 323
oleacinum, C. 323
oleae, Aureobasidium 307
oleae, C. 323
oligocarpum, C. Didymotrichum 323
oligocarpum var. malvacearum, C. 323
olivaceum, Amphi. 340
olivaceum, C. Mydonosporium 358
oncobae, C. 14, 35k, 41k, 56k, 195*−196*, 223
onobrychidis, H. 369
opacum, C. Cladotrichum 323
ophiopogonis, C. 37k, 358
oplismeni, C. 359
opuntiae, H. 155
orbiculans, C. 323
orbiculata, Annellosympodia 324
orbiculatum, C. F. 323
orchidearum, C. 33k, 40 k, 86, 169, 186, 196−198*, 199, 205, 232,
236
orchidiphilum, C. 13, 33k, 40 k, 198*
orchidis, C. F. 198, 324*
oreodaphnes, C. 11−12, 32k, 39k, 89, 199−200*, 247
ornithogali, C. D. H. Didym. 31k, 37k, 39k, 53, 200*−201, 369
ornithogali var. allii-porri, H. 53
ornithogali var. minus, H. 200
oryzae, C. 355, 357, 359
ossifragi, C. H. N. 16c, 19t, 31k, 33k, 40k, 201*−203, 250
osterici, C. 324
oudemansii, C. 324, 326
ovorum, C. 359
oxybaphi, H. 183
oxycocci, C. St. Z. 324*
oxysporum, C. 3, 12, 17, 25k, 28k, 32k, 42k, 79, 95, 108, 133−134,
186, 203−206*, 258
P
paeoniae, C. 4, 307, 324
paeoniae var. paeoniae-anomalae, C. 307, 324
paeoniae-anomalae, C. 324
pallidum, C. Csp. 359
pallidum, C. Horm. 359
palmarum, Coniothyrium 9c
palmetto, C. 325
palmetto, Helm. 325
pannosum, C. 26k, 206*
388
papyricola, C. 250
paracladosporioides, C. 16c, 19t, 29k, 120, 206−208*, 228, 283
paradoxum, H. Laocoön 337
paraguayensis, P. 10c
Parahaplotrichum 24k
Parapericoniella 4, 23k, 300
Parapleurotheciopsis 24k
parasitica, Alysidiella 10c
parasitica, F. Monotospora 326
parasitica, Peronospora 261
parasiticum, C. Strumella 325*
Passalora 4, 13, 24k, 56, 125, 163, 272, 309, 346, 369
patouillardii, Csp. 304
paulensis, Csp. 326
paulliniae, C. St. Z. 325*
paulsenii, H. 337
pedrosoi, Fonsecaea Phialophora 297
pelliculosum, C. 325
peltigerae, Capronia 9c
penicilloides, C. 359
Penidiella 4, 23k, 199
perangustum, C. 12, 16c, 19t, 28k −29k, 93, 196, 208−210*
pereffusa, Streptothrix 327
perfoliati, Csp. Mycovel. Pass. 334
pericarpium, C. 325
Periconiella 23k, 31, 40, 43-44, 266, 366
peridermiicola, C. 134−135, 180, 276
perpusillum, C. 359
persicae, St. 325
persicum, C. 325
persicum, Clasterosporium 325
personata, M. 335
personata var. cassiae-occidentalis, Csp. 326
personatum, C. Csp. Csporid. Cercosporiopsis Pass.
Phaeois. 325−326
personatum f. arachidis-hypogaeae, C. 326
personatum var. cassiae, C. 326
peruamazonicum, C. 359
peucedani, F. 309
pestis, C. 326, 330
petraeum, Dem. 300
petuniae, H. 183
Phaeoblastophora 23k
phaenocomae, C. 16c, 19t, 28k, 37k, 210−211*
Phaeoramularia 24k, 369
phaseoli, C. 326
phaseolorum, Diaporthe 9c
phlei, C. H. 16c, 19t, 31k, 41k, 57, 211−213*, 352, 360
phlei-pratensis, C. 360
phoenicis, C. 85, 182, 184
phragmitis, C. H. 324, 326, 369
phragmitis var. ammophilae, H. 337
phragmitis var. inlorescentiae, H. 369
phragmitis var. typharum, H. 182
phyllachorae, C. 326
phyllactiniicola, C. 16c, 19t, 28k −29k, 43k, 213*−215, 217
phyllogenum, C. 281−284
phyllophilum, C. 16c, 19t, 29k, 43k −44k, 140, 158, 175, 215*−217,
267, 349
phyllophilum, Helm. 155
Phyllosticta 152
pilicola, C. 133, 360
the genuS Cladosporium
pini, Dendryphion 329
pini, Rachicladosporium 10c
pini-ponderosae, C. 16c, 19t, 28k, 40k, 119, 218−220, 288
pipericola, C. 34k, 40k, 220*−221
piricularioides, C. 326
piriforme, C. 361
pirorum, C. 328
pisi, Ascochyta 9c
pisi, C. 172, 173, 360
pisicola, C. 35k, 39k, 93, 173, 221*−222, 350
pithecolobii, C. 326
platycodonis, C. 38k, 360
Pleurophragmium 325
polygonati, C. H. 30k, 37k, 222*, 360
polygonaticola, C. 37k, 360
polygonorum, Csp. P. 309
polymorphosporum, C. 326
polymophum, Stempylium 296
polymorphum, C. 327, 370
Polyscytalum 22k, 25k
polysporum, C. 327
polysporum, Acladium Chloridium 101
polythrincioides, Pass. 309
polytrichorum, C. 360
pomi, F. Spilocaea 117, 130, 309, 324, 328
pomi, Pass. 327
populi, Clasterosporium 318
populi, Clasterosporium Stig. 318
populi, Fusariella 299
populicola, C. 13, 15, 33k −35k, 41k, 60, 138, 175, 222−223*
populinum, Dicoccum 318
porrigo, Csp. 327
porophorum, C. 321, 327
potebniae, C. 361
potulentorum, Cladophial. 8*
praecox, C. F. 13, 33k, 37k, 49k, 85−86, 169, 178, 186, 190, 195,
198, 223−224*, 236
profusum, C. 361
profusum f./var. robustior, C. 155, 361
proteae, F. 5*
proteus, H. 183
pruneti, H. 166
pruni, F. 304
prunicola, C. 361
psammicola, C. Ex. F. 327*
pseudiridis, C. 16c, 19t, 31k, 39k, 224−226*
Pseudocercospora 4, 125, 175, 330
pseudocladosporioides, C. 16c, 19t, 30k, 129, 210, 226*−228, 276
Pseudocladosporium 22k, 24k −25k, 333
pseudoplatani, H. 155
psidiicola, C. 361
psoraleae, C. 36k, 39k, 81, 126, 228*−229, 290, 339, 354
psychrotolerans, C. 16c, 19t, 26k, 44k, 229−230*
puccinioides, C. Prathigada Pseudoasperisporium 327, 328*
pulcherrimum, C. 327
pullulans, C. Dem. O. Aureobasidium Hormonema Oospora
Pullularia 307, 312, 327, 328, 334, 369, 370
pullulans var. pullulans, Aureobasidium 307, 327
pulmonaria, Beverwykella 9c
pulvinata, Botrytis 153, 366
punctatum, C. 155, 343, 361
punctiforme, C. Csp. 125, 328
www.studiesinmycology.org
punctiformis, Csp. 328
punctulatum, C. 155, 157
punctulatum var. xylogenum, C. 361
pustulata, Lasallia 9c
putrefaciens, C. 328
putrefaciens, Clasterosporium 328
pygmaeum, C. 328
Pyricularia 2
pyriforme, C. 361
pyrina, V. 327
pyrinum, Arthrinium 327
pyrinum, Fusidium 327
pyrorum, C. 328
pyrorum, F. Helm. Megaclad. Pass. 327, 362
pyrorum f. carpophila, F. 327
pyrorum var. cladophilum, F. 327
Q
qinghaiense, C. 39k, 361
quitense, C. St. Z. 328*
R
Rachicladosporium 4, 23k
radians, C. 328−329*
radiosum, F. O. Stig. Poll. V. 299, 318, 329
radiosum var. lethiferum, F. Poll. 318
radiosum var. microsporum, F. 299
radiosum var. radiosum, F. 299, 329
ramotenellum, C. 16c, 19t, 27k, 93, 97, 230−232*, 269
Ramularia 22k
ramulosum, C. F. Poll. 223, 299, 329, 362
raphanicola, C. 362
ravenelii, Bipolaris 336
ravenelii, Helm. 257
rectangulare, C. 12, 34k, 40k, 232−233*, 235
rectoides, C. 14, 16c, 19t, 30k, 233−235*
rectum, C. 329
repandum, H. 337
resinae, C. Horm. Amorphotheca Dendryphion Hormoconis
Pycnostysanus Racodium Sorocybe Sporocybe Stysanopsis
Stysanus 4, 5*, 7*, 9c, 24k, 300, 303, 329
resinae f. albidum, C. 300, 329
resinae f. avellaneum, C. 300, 329
resinae f. sterile, C. 300, 329
resinae f. viride, C. 329
Rhizocladosporium 4, 24k
rhododendri, C. 32k −33k, 38k, 121, 235*−236*, 329
rhodomyrti, C. 329
rhoina, P. 286
rhois, C. 329
rhois-coriariae, Csp. 330
rietmanni, C. Hortaea 330, 335
rigidiphorum, C. Pen. 330
rivinae, C. 34k, 40k, 236−238*
robiniae, C. H. 31k, 38k, 238*
robinsonii, Antennaria 313
roesleri, C. Csp. Ragnhildiana 326, 330
roesleri f. fuckelii, Csp. 330
roesleri f. typica, Csp. 330
romellianum, F. 320
389
BenSch et al.
rubella, Leptospora 9c
rubi, P. 315
rubrigenum, Toxicocladosporium 10c
rutae, C. R. 13, 33k, 41k, 239*
S
sagittariae, Csp. 316
salicina var. tirolense, R. Phacellium Ramulaspera 330
salicis, C. F. 330*
salicis-sitchensis, C. 330
salinae, C. 16c, 19t, 26k, 44k, 239−241*
sambuci, C. 330, 362
sambuci, H. 337
sambucigena, P. 330
sanguinea, Csp. 320
saniculae-europaeae, Csp. P. 125, 328
sapientumicola, Periconiella 323
sarcopodioides, C. Clasterosporium 330
sarmentorum, C. Acrosporella 12, 26k, 193, 241*
sarraceniae, C. 155
savastani, C. 362
savulescui, H. 370
scabies, C. 99
scabrellum, C. 16c, 19t, 28k, 119, 210, 242*−243*
scarioliae, Pass. 317
scillae, C. F. Cladophial. 330, 331
sclerotiophilum, C. 98, 331
Scolecobasidium 337
scolecotrichoides, Csp. 307
scopiforme, C. Helm. Pleurophragmium Spiropes 308, 331
Scopulariopsis 318
scribnerianum, C. F. 331
secedens, C. 362
secalis, H. 337
Seifertia 23k
selaginellarum, H. 337
septica, Byssus 305
Septoidium 334
Septonema 24k
Septoria 152
sericeum, C. 331
shelburniensis, Devriesia 10 c
sidae, C. 331
silenes, C. 16c, 19t, 38k, 243−245*
simplex, C. 127, 362
simplex, Cladotrichum 299
simplex, Trichosporum 367
sinuosum, C. 12, 16c, 19t, 25k, 245*−246*
smilacicola, C. 12, 32k −33k, 42k, 245−247*
smilacis, C. Dem. 245, 247, 362
solanicola, C. Mycovel. 331
solaninum, Heteroconium 332
soldanellae, C. 12, 16c, 19t, 33k, 41k, 247−250*
solidaginis, Ascospora 316
solutum, C. 362
somalensis, Csp. 326
Sorocybe 23k −24k
sorghi, C. 204−205
sorghi, H. 155
sparsum, C. 153, 157
Sphaceloma 343
390
Sphaeridium 22k
sphaeriiforme, H. 183
sphaeroidea, Csp. Phaeois. 326
sphaeroideum, C. 331
sphaerospermum, C. 2−3, 11−12, 14, 16c, 17, 19t, 25k −26k, 42k,
44, 110, 131, 140−141, 146, 172, 210, 229, 250−253*, 256,
258, 260, 269, 286, 293, 331, 351
sphaerosporum, C. 331
sphondylii, C. 331
spinae-christae, C. 367
spinulosum, C. 16c, 19t, 26k −27k, 254*−256, 260
spiraeae, H. 155
Spiropes 325
splenicum, Trichosporum 319
spongiosum, C. Helm. 35k, 41k, 107−108, 256*−257, 347
Sporocladium 4
stanhopeae, C. 33k, 40k, 257*−258*
staurophorum, C. Horm. Devriesia 4, 331
Stemphylium 369
Stenella 4, 11−12, 24k, 67, 128, 174, 192, 199, 297, 305, 345, 356
stenhammariae, H. 370
stenosporum, C. 362
stercorarium, C. 362
stercoris, C. 363
stipae, C. St. Z. 331−332*
straminicola, C. 363
strictum, C. Cladotrichum 331
strigosum, Dem. 300
strobilanthis, C. 36k, 363
stromatigenum, H. 155
stromatum, C. 193, 331, 363*−364*
strumelloideum, C. Pen. 332
stysanoides, C. 247−250
suaveolens, C. Candida Geotrichum Moniliella Oospora
Sachsia 306, 332
subcompactum, C. 343, 364
subcoronatum, Botryobasidium 9c
subfusoideum, C. 98, 364
subinlatum, C. 12, 16c, 19t, 25k, 258*−260
subnodosum, C. 277−278, 281
subobtectum, C. 34k, 38k, 260*−261
Subramaniomyces 24k
subsclerotioideum, C. 35k, 38k, 261*−262
subsessile, C. F. 223, 302, 320, 332
subtile,C. 203
subtilissimum, C. 16c, 19t, 27k, 71, 97, 147, 262*−264, 269
subuliforme, C. 16c, 19t, 29k, 58, 101, 264*−265*
supericiale, C. 332
sweetiae, Pass. 311
sycophilum, C. 364
symphoricarpi, C. Csp. Pass. Phaeo. 332
symphoricarpi, H. 370
syphiliticum, C. Coniothecium Penicillium 332
syringae, C. 267, 364
syringae, H. 155
syringae-japonicae, Erysiphe 265
syringicola, C. 15, 31k, 40k, 175, 265−266*
T
tabaci, C. 358, 364
Taeniolella 22k, 298, 310, 334
the genuS Cladosporium
Taeniolina 22k
tapesiae, Cladotrichum 300
taphrinae, C. 43k −44k, 267*−268, 349
tassiana, D. M. Sphaerella 11, 52, 152*, 154, 157
tassiana var. arthropyrenoides, M. 244
tectonae, C. 332−333
tectonicola, C. F. Z. 1, 332−333
tenellum, C. 12, 16c, 19t, 27k, 232, 268*−269*
tenerrimum, C. 365
tenerum, C. 333
tenuis, C. 333
tenuissimum, C. 12−13, 16c, 20t, 26k, 28k−30k, 35k, 43k, 92−93,
95, 97, 222, 227, 243, 258, 265, 269*−272, 276, 283, 347, 357
Teratosphaeria 4, 17, 43
terrestre, H. 338
terricola, Pidoplitchkoviella 9c
tetrapanacis, C. 36k, 365
teucrii, C. 39k, 365
thailandica, Hortea 6*
thapsiae, H. 183
theobromicola, C. 365
thermodurans, Devriesia 10c
thouiniae, P. 353
tomentosum, C. 153
tortuoso-inlatum, H. 183
tortuosum, C. 365
Torula 200
Toxicocladosporium 4, 24k
trabutiana, Bispora 357
transchelii, C. 365
transchelii var. semenicola, C. 365, 367
transchelii var. viridi-olivacearum, C. 365, 367
tremulae, F. N. V. 299
tremulae var. grandidentatae, V. 318
Trentepohlia 2, 300
trichellum, C. 333
trichoides, C. 300, 333
trichoides var. chlamydosporum, C. 300, 333
trichophilum, Chaetotrichum 333
trichophilum, C. Mycovel. 318, 333
trichostematis, H. 183
trillii, C. H. 12, 31k −32k, 39k, 272−274*
trillii, Phyllosticta 272, 274
trilliicola, C. 273−274
Trimmatostroma 127, 267, 313
triostei, C. F. 334*
tropaeoli, H. Acroconidiella 338
tropicale, C. 334
tschawytschae, H. Ochroconis Scolecobasidium 338
tuberculans, H. 183
tuberculatum, C. 365
tuberum, C. 102−103, 106
tulasnei, M. 184
tulasnei, Sphaerella 348
tupae, H. 370
typhae, C. 153
typharum, C. 147, 153
typharum, H. 181
typharum f. fuscum, C. 334
typharum f. lanciforme, C. 181
typharum f. minor, C. 366
typharum var. fuscum, C. Brachysporium 334
www.studiesinmycology.org
U
uleanum, C. Septoidium 1, 334
ulmariae, C. 366
umbelliferarum, H. 370
umbrinum, C. 366
unamunoi Csp. Phaeo. 304
unedonis, C. 334
uniseptatum, Cladotrichum Diplococcium Scol. Virgaria 299
uniseptosporum, C. 366
uredinicola, C. 10c, 27k, 43k −44k, 128, 138, 196, 215, 257, 272,
274*−276*, 294, 356, 366
urediniphilum, C. 366
urostigmatis, Pass. 346
ushuwaiense, C. 11, 13, 34k, 37k, 130, 131, 276-277*
uvarum, C. 366
V
vagans, C. Fumago 312, 334
vangueriae, C. St. Z. Biharia 334
variabile, C. H. Helm. 12, 16c, 20t, 31k, 38k, 95, 133, 182, 187, 274,
277−281*
variabilis, D. 277
varians, C. 12, 16c, 20t, 26k, 29k, 34 k, 37 k, 42 k, 208, 281*−284*
vellosoanum, H. 370
velox, C. 16c, 20t, 26k, 284−286*
velutina, Periconiella 10c
velutinum, C. 155, 157
Venturia 1, 3−4, 22k, 25k, 335
venturioides, C. 366
venturioides, Csp. Mycovel. Pass. 322
venturioides var. citricola, C. 367
verrucocladosporioides, C. 14, 16c, 20t, 27k, 36k, 75, 136, 286*−288
Verrucocladosporium 4, 24k, 288
versicolor, C. 334
vesicarium, Helm. 346
vesiculosum, Helm. Brachysporium 155
victorialis, C. Csp. 17, 32k, 36k, 70, 288*−289*
vignae, C. 27k, 36k, 39k, 58, 81, 139, 229, 288−291*
vincae, C. 155, 157, 291
vincae, R. 157
vincetoxici, Csp. 301
vincicola, C. 33k, 36k, 291*
vinosa, Dendr. 198, 305, 337, 368
“virescens”, C. Dem. Sporotrichum 335
virescens, F. 327
virens, Septocylindrium 330
virgultorum, C. 335
viride, C. Horm. Penicillium 367
viridiolivaceum, C. 365, 367
viridiolivaceum var. semenicola, C. 365, 367
viticola, C. Csp. 335
vitis, C. Csp. Helm. P. Cercosporiopsis Phaeoisariopsis
Septonema 10c, 297, 335
vitis f. parthenocissi, Csp. 335
vitis var. rupestris, Csp. 335
vitis-frutigeni, C. 367
vulgare, Dem. 152
vulgare (alpha) herbarum, Dem. 152
vulgare (beta) foliorum, Dem. 181, 184
vulgare (delta) typharum, Dem. 181, 184
391
BenSch et al.
vulgare (gamma) fungorum, Dem. 153
vulgaris, Epicoccum 157
W
Websteromyces 23k
werneckii, C. Dem. Exophiala Hortaea Phaeoannellomyces
Pullularia 321, 330, 335
whetzelii, Csp. Piricularia 315
wikstroemiae, C. H. St. Z. 1, 335, 338
X
xanthosomatis, Csp. H. Helm. 370
Xylohypha 22k, 310
xylophilum, C. 16c, 20t, 30k, 75, 193, 242, 291−293*
xyridis, C. 35k, 42k, 293−294*
392
Y
yuccae, C. 33k, 37k, 295*
yuccae, H. 155
Z
Zasmidium 4, 11−12, 24k, 128, 174, 199, 305, 319, 345
zeae, C. 335, 367
zeylanicum, C. 343
zizyphi, C. Macrosporium 335, 367
the genuS Cladosporium
INDEX TO HOSTS AND SUBSTRATES
A
Abelia 319
Abelmoschus 64, 253, 353, 362
Abies 134–135, 180, 310, 328
Acacia 73, 210, 272, 296
Acalypha 38, 45–46
Acanthaceae 36, 321, 363
Acer 72, 93, 173, 175, 185, 227, 307, 316, 334, 339, 363
Aceraceae 93, 173, 185, 227, 339
Acosmium 311
Acrosporium 257
Actinidia 52
Actinidiaceae 52
Adenanthera 205
Adenia 336
Adenophora 360
Adiantum 296
Adoxaceae 93, 165, 193, 330, 337, 362, 370
Aecidium 48
Aesculus 33, 41, 70
Agaricaceae 114
Agaricales 44, 115, 157, 178, 366
Agaricus 114, 157, 366
Agavaceae 73
Agave 348
Ageratum 334
Agoseris 31, 37, 49, 195, 224
Agrimonia 228
Agrocybe 366
Agrostis 368
Ailanthus 344
air 1, 52, 90–91, 93, 106, 146, 157, 165, 205, 226–227, 232, 253,
272, 293
airconditioning system 52
Aizoaceae 349
Albizia 336, 339
Alcea 275–276
Alhagi 368
Alisma 72
Alismataceae 72, 227, 316
Allium 17, 31–32, 36, 39, 50, 53–55, 65, 157, 169, 275, 288, 339
Alloxylon 41, 57, 58
Alnus 23, 35, 37, 55–56, 157, 165, 196, 300, 349
Aloe 228
Alopecurus 31, 41, 57
Alpinia 42, 339
Alstroemeriaceae 336
Althaea 184
Amanita 157
Amanitaceae 157
Amaranthaceae 92, 341, 366, 368
Amaranthus 366–367
Amaryllidaceae 36, 39, 50, 53–55, 157, 169, 288, 339
Amaryllis 257
Ambrosia 340
Amelanchier 275
Ammophila 327, 337, 359, 369
Amorpha 297
Ampelodesmos 340
www.studiesinmycology.org
Anacardiaceae 36, 286, 298, 323, 329, 351
Andromeda 121
Andropogon 72, 157
Anemone 48
Angelica 33, 36, 124–125, 309
Annona 35–36, 59–60
Annonaceae 36, 59
Anthemis 348
Anthostema 322
Anthracoides 232
Anthyllis 64
Antirrhinum 359
Aonidia 354
aphid 22, 44, 62–64, 351
Aphis 62, 64
Apiaceae 36, 48, 73, 124–125, 309, 318, 324, 328, 331, 370
Apiosporina 180, 293
Apocynaceae 36, 64, 157, 190, 291, 301, 304, 322, 356–357, 368
Aquifoliaceae 36, 85
Arabis 35, 38, 261, 340
Araceae 36, 93, 95, 108, 175, 304, 306, 336
Arachis 205, 325, 326
Aralia 297
Araliaceae 36, 52, 106, 157, 208, 297, 365
Arbutus 65, 334
Areca 93, 257
Arecaceae 36, 77, 85, 93, 98, 264, 303
Argemone 275–276, 315
Aristolochia 37, 340
Aristolochiaceae 37, 340
Armeniaca 361
Armillaria 114–115
Arnebia 337
Arrhenatherum 52
Artemisia 34, 37, 194–195, 261, 275–276, 298, 312
Arthonia 298
Arthoniaceae 298
Arthropodium 31, 37, 39, 67, 70
Arum 275
Arundinaria 307
Arundo 93, 253, 340
Asclepias 64, 322
Ascyrum 314
Asparagaceae 37, 39, 48, 52, 67, 110–111, 169, 200, 206, 222,
242, 295, 311, 330, 348, 353, 358, 360, 368–369
Asparagus 311, 348
Aspleniaceae 37, 353, 358
Asplenium 353, 358
Asplundia 64
Aster 298
Asteraceae 37–48, 49, 52, 64, 73, 79, 81, 93, 101, 163, 194–195,
210, 223–224, 227–228, 272, 296, 298, 301, 305, 308, 311–
313, 315, 317–318, 321, 334, 337, 340, 343, 348, 368
Asterina 23, 298
Astragalus 48
Atriplex 341
Aureobasidium 146, 180
Avicennia 321
B
Baldingera 348
Balladyna 300
393
BenSch et al.
bamboo 92, 285 (also see Bambusa)
Bambusa 284, 348 (also see bamboo)
banana 4, 23, 272 (also see Musa)
Baptisia 300
Basella 272
Basellaceae 272
Batrachium 132
Begonia 162
Belamacanda 168
Berberidaceae 37, 48, 272, 276, 328
Berberis 34, 37, 48, 130, 175, 276–277, 328
Berkheya 301
Beta 328, 368
Betula 193, 301, 303, 313, 331, 359
Betulaceae 37, 55, 64, 119, 131, 157, 165, 180, 193, 210, 213,
229, 300–301, 303, 305, 312–313, 327, 331, 349
Bignonia 158, 341
Bignoniaceae 37, 157, 169, 281, 317, 341
bing cherry fruits 93, 264, 293
biomats 144, 172
Boenninghausenia 342
Boletaceae 157
Boletales 43–44
Boletus 116, 157, 180
Bomarea 336
Bombax 348
Boraginaceae 38, 62, 64, 147–148, 336–337, 370
Borassus 31, 36, 77
Boscia 35, 38, 77, 79, 176
Bougainvillea 34, 40, 67
Brachyelytrum 302
Brassica 31, 38, 64, 79, 184, 342, 351
Brassicaceae 38, 64, 79, 184, 261, 327, 342, 351, 362
bread 272 (also see food)
Bromeliaceae 180
Bromus 114
Brunneosphaerella 93
Buddleja 64, 352
building material 91, 93, 106
Butea 34, 38, 80
butter 303 (also see food)
C
Cactaceae 361
Caesalpinia 303
Cajanus 337
Calamagrostis 213
Calamus 303
Calea 337
Calla 304
Callistemon 272
Caloplaca 42–43, 60, 62
Calotropis 304
Calycanthus 359
Campanula 48
Campanulaceae 38, 48, 325, 360, 369–370
Campovassouria 311
Campsis 341
Canavalia 101
Canis 306
Cannabaceae 312
394
Canthium 300
cap fungi 116
Capnodiales 43
Capparaceae 38, 77, 175
Capparidaceae 176
Capparis 32, 35, 38, 175
Caprifoliaceae 48, 64, 310, 319, 332–334, 369–370
Capsicum 101, 304
Caragana 34, 36, 39, 80–81, 368
Carex 157, 232, 275, 332
Carica 38, 272, 342
Caricaceae 38, 272, 342
Carludvicia 64
Carpesium 35, 37, 81
Carpinus 157, 305, 310, 312, 327
Carya 253, 305, 309
Caryophyllaceae 31, 38, 52, 92, 106, 111, 113–114, 243, 341, 355
Cassia 32, 35, 81, 83, 257, 326, 340
Castanea 73, 102, 345
Catalpa 37, 281, 283
Cattleya 198, 305
Celastraceae 38, 64, 106, 157, 253, 260, 346, 361, 368
Celastrus 368
Cellulose powder 92
Celosia 92
Celtis 275
Cenchrus 35, 41, 256–257
Centaurea 275, 368
Cerastium 114
Cercestis 306
Cestrum 274–275
Cetraria 175
Chaetochloa 35, 41, 257
Chamaecrista 32, 35, 39, 83
Chamaedorea 264–265
Chamaerops 33, 36, 85
cheese 305 (also see food)
Cheiranthus 348
Chenopodiaceae 38, 92–93, 180, 277
Chenopodium 92
Cherry 218
chicken food 131
Chionanthus 157, 313
Chloris 336
Chlorogalum 169
Chlorophytum 359
Chrysanthemum 228, 257, 275, 342–343
Chrysocyclus 275
Chrysophyllum 31, 41, 87
Chrysothamnus 48
Cinnamomum 199, 307, 332
Circaea 40, 343
Cirsium 93
Cissampelos 356
Citrullus 38, 100, 101
Citrus 32, 41, 97–98, 110, 145, 157, 165–166, 205, 250, 253, 272,
303, 307, 310, 331, 343, 346–347, 364, 367, 369
Cladrastis 307
Clavicipitaceae 114
Clusia 297
Clusiaceae 297
Coccidae 353
the genuS Cladosporium
Coccinia 38, 100
Coffea 315
coffee leaf 93, 228
Coix 257
Coleoptera 325
Coleosporium 48
Collembola 253
Colocasia 28, 32, 36, 93, 95, 133, 336
Combretaceae 343
conifer wood 144, 172
conifers 348
Consolida 364
contaminant 144, 146, 157, 253, 318
Convolvulaceae 106
Coprosma 275–276
Corchorus 39, 344
Cordyline 33, 37, 110–111, 353
Coreopsis 308
Cornaceae 308, 351
Cornus 308, 351
Cortaderia 28, 52, 95, 97, 165
Corylaceae 185, 269
Corylus 17, 64, 119–120, 131, 157, 165, 180, 185, 210, 213, 229,
269, 275, 312
Corymbia 32, 40, 188–189, 210
Corypha 30, 36, 98
cosmetics 24
Cotinus 351
cotton duck 253
Crassulaceae 253, 348
creosote 4, 24, 228, 300, 329
Crepidiastrum 79
crested wheat grass 93
Cronartiaceae 134, 276
Cronartium 43–44, 134–135, 275–276
Crotalaria 369
Cucumis 38, 98, 100–101, 205
Cucurbita 38, 100–101, 368
Cucurbitaceae 29, 35, 38, 98, 100–101, 205, 290, 316, 345, 368
cucurbits 101, 345
Cullen 228–229, 290
Cussonia 208
Cycadaceae 38, 64–65, 101–102
Cycas 32–33, 38, 64–65, 101–102, 130, 277
Cyclamen 308
Cyclanthaceae 64
Cynanchum 301
Cynosurus 308
Cyperaceae 106, 157, 184, 297, 315, 332
Cyperus 297
Cypripedium 33, 40, 198–199
Cyrtomium 38, 344
Cytospora 365
Cyttaria 308
Cyttariaceae 308
D
Dacrydium 205
Dactylis 114, 369
Dactylorhiza 198, 324
Dahlia 313
www.studiesinmycology.org
Dalbergia 92, 272
Daphniphyllaceae 308
Daphniphyllum 308
Datura 349
decaying material 52, 91, 116, 157, 180, 184, 253, 272, 297, 315,
345, 358, 364
Delphinium 157, 364
Dennstaedtiaceae 340
Desmodium 310, 317, 344
Dialium 298
Dianella 42, 336, 344–345
Dianthus 31, 38, 52, 106, 111, 113, 341, 355
Diaspididae 354
Dichanthium 72
Dieffenbachia 31, 36, 108
Digitalis 42, 345
Diospyros 318
Dipterocarpaceae 272
Dirina 24
Doellingeria 298
Dolichos 352
downy mildew 43, 275–276
Dracaena 33, 37, 110–111
Dracophyllum 253
Dryopteridaceae 38, 344
dung 348, 362–363
dust 106, 165
E
Ebenaceae 318
Echeandia 206
Echeveria 348
Echium 62, 64
Edgeworthia 42, 345
egg 359 (also see food)
Elaeagnaceae 309, 345
Elaeagnus 48, 309, 345
Elsinoaceae 310
Elsinoë 310
Elymus 213
Endocronartium 43–44, 134, 138
Enkianthus 359
Epacris 310
Ephedra 351
Ephedraceae 351
Epichloë 42–43, 48, 114
Epidendrum 34, 40, 232
Epilobium 48, 340
Equisetaceae 184
Equisetum 184, 352
Eragrostis 343
Eremostachys 368
Erianthus 310
Ericaceae 38, 120, 235, 310, 324, 329, 334
Eriobotrya 31, 33, 41, 116–117, 130, 266, 358
Eriodictyon 336
Eriolobus 310
Eriophorum 184
Erysiphaceae 185, 269
Erysiphales 43, 64, 92, 119, 131, 165, 180, 210, 213, 215, 229,
257, 275
395
BenSch et al.
Erysiphe 43, 253, 265, 275–276
Erythrina 340
Erythrochiton 64
Erythrophleum 210
Eschscholtzia 310, 336
Eucalyptus 40, 52, 71, 74–75, 91–92, 95, 117, 119, 189–190, 210,
227, 257, 264, 297, 345, 368
Eugenia 313
Euodia 48
Euonymus 34, 38, 64, 106, 157, 253, 260–261, 275–276, 346, 361
Eupatorium 311
Euphorbia 47–48, 306, 345
Euphorbiaceae 38, 45, 47–48, 64, 92, 192–193, 306, 322, 344,
346, 362, 369
Eutypa 363
Exoascus 215, 217, 349
Exobasidiales 44, 120–122
Exobasidium 42, 44, 120–122
gill fungi 43, 115–116
Ginkgo 157
Ginkgoaceae 157
Gladiolus 52, 168, 169, 346, 369
glass 352
Glebionis 73, 228
Gleditsia 35, 39, 139
Glochidion 347
Glypholecia 175
Gomphidius 180
Gossypium 92, 314, 347–348
Gramineae 307
grape 17, 71, 93, 262, 264 (also see Vitis)
Grevillea 41, 142, 144
Grewia 348, 356
Grossheimia 275–276
Grossulariaceae 308, 312
Gymnosporangium 275–276
Gynoxys 32, 37, 163, 195, 315
F
Fabaceae 38, 64, 73, 80–81, 83, 92, 101, 106, 128, 139, 172, 177,
193, 205, 210, 221, 228, 238, 272, 288, 290, 293, 296–298,
300, 303, 307, 310–311, 316–317, 325–326, 336–337, 339–
340, 344, 349–350, 352, 354, 360–362, 366, 368–370
Fagaceae 39, 52, 73, 127, 184, 267, 276, 298, 302–303, 308, 312,
317, 343, 345, 354, 356, 365
Fagus 193, 302
Falcaria 48
Fatoua 93
ferns 313
Festuca 157, 346
Ficus 129, 146, 205, 308, 326, 346, 364
Filipendula 366
Flacourtiaceae 196
lax 355
Flueggea 311
Foeniculum 73
food 91, 93, 131, 145, 157, 165, 208, 227, 253, 272, 303, 305,
307, 343, 345, 359, 362
Formica 358
Forsythia 40, 346–347
Fragaria 312
Frangula 48
Fraxinus 36, 40, 92, 125–126, 157, 175, 312, 315, 344, 362
Freesia 169, 257
fruit bodies 43–44, 184, 227, 312, 357, 366
Frusinalia 343
Fuchsia 245
Fumaria 342
Funkia 368
G
Gagea 31, 201
Galium 32, 41, 132–133, 360
Gardenia 229
Gemmingia 168
Gentiana 33–34, 39, 136, 138
Gentianaceae 39, 136, 138
Georgina 313
Gerwasia 43–44, 138
396
H
Haematomma 319
Haplophragma 157–158
Haplophyllum 31, 41, 146
hay 355, 363
Hedera 52, 106, 157
Hedysarum 48
Helenium 48
Helianthus 101, 340
Heliotropium 34, 38, 147–148
Helotiales 43
Hemerocallis 168–169
Hemileia 315
Hemiptera 353–354
Heracleum 331
Hesperostipa 331
Heteromeles 309
Heterophragma 34, 37, 157
Hibiscus 253, 353, 362
Holcus 114, 213
Homo sapiens 309 (also see man)
Homogyne 52
honeycomb 366
Hordeum 4, 48, 50, 52, 75, 77, 152, 184–185, 238, 245, 351
Hosta 368
Hovea 316
Humulus 312
Hydrangea 39, 90, 353
Hydrangeaceae 39, 90, 353
hydrocarbon-rich 24
Hymenoxys 48
Hypericaceae 313
Hypericum 313–314
Hypholoma 115, 157, 184
Hypocreales 43
Hypogymnia 175
Hypoxylon 310
I
ice, arctic 144–145, 172
the genuS Cladosporium
Idesia 184
Ilex 33, 36, 85–86
Indigofera 293
indoor environment 17, 90, 93, 106, 144–146, 157, 205, 229, 253,
293, 305, 322, 332
Ipomoea 106
Iridaceae 39, 52, 166, 168–169, 184, 224, 346, 353, 368–369
Iris 31, 39, 166, 168–169, 184, 224, 226, 353
Isomeris 73
Ixeris 79
Ixora 323
J
Jacaranda 32, 37, 158, 169, 190, 236, 317
Jasminum 359
jet-fuel 24
Juglandaceae 73, 253, 305, 309, 325, 353
Juglans 73, 325, 353, 363
Juncaceae 353
Juncus 353
Juno 168
K
Kalanchoë 253
Kentucky coffee tree 228
Kniphoia 54, 185
Kydia 355
L
Laciasis 315
Lactarius 115, 180, 366
Lactuca 317
Lagenaria 38, 100–101
Lagerstroemia 269
Lallemantia 369
Lamiaceae 39, 92, 235, 322, 332, 365, 368–369
Lantana 318, 333
Larix 157, 369
Laserpitium 318
Lasius 356, 358
Lathyrus 39, 296, 354
Lauraceae 39, 199, 307, 311, 320, 332, 354
Laurocerasus 108
Laurus 200, 354
Leaf litter 102, 146, 283
Lecanium 354
Lecanora 175
Lepiota 180
Lespedeza 36, 139, 290
Leucopaxillus 115
Leycesteria 319
lichen 24, 27, 29, 42, 60, 62, 93, 147, 173, 175, 253
Ligustrum 48, 161
Liliaceae 39, 201, 323, 352, 358, 360
Lilium 169
Limonium 48
Linaceae 355
Liriodendron 35, 39, 176–177
Lithocarpus 356
www.studiesinmycology.org
Litsea 307
Lobelia 309, 325, 369–370
Lonicera 48, 64, 319, 333, 369
Lophodermium 355
Loxospora 319
Luffa 38, 100–101
Lupinus 32, 39, 177–178
Lychnis 38, 114, 355
Lycoperdon 178, 180
Lycopersicon 312, 347, 355
Lycopodiaceae 157
Lycopodium 157
Lysimachia 320
Lythraceae 269, 320
Lythrum 320
M
Maackia 307
Machilus 199, 320
macromycetes 43–44
Macrotomia 337
Magnolia 26, 32, 39, 65, 93, 102, 184, 210, 272, 331, 337, 344
Magnoliaceae 39, 93, 102, 176, 210, 272, 331, 337, 344
Mahonia 272
Malope 320
Malus 48, 217, 227, 308, 320, 345, 361–362, 365
Malvaceae 39, 64, 92, 184, 253, 297, 314, 320, 323, 331, 343–
344, 347–348, 353, 355, 362, 365
man 1, 3, 25, 52, 253, 296–297, 305, 320–321, 332–335 (also see
Homo sapiens)
man, allergy 1
man, brain 146, 300, 333
man, breast 309
man, chromoblastomycosis 305
man, gonorrhea 314
man, lesion 144, 172, 314, 318
man, mycoses 1, 172
man, nail 250, 253
man, nasal mucus 172, 253
man, skin 52, 321, 330
man, sputum 52
man, uterus 304
Manihot 32, 38, 64, 192, 369
margarine 210 (also see food)
meat 345 (also see food)
meat stamp 139, 140
Medicago 369
Melaleuca 40, 123–124
Melampsora 48, 275
Melampsoridium 227
Melanthiaceae 39, 272
Meliaceae 65
Melinis 257
Melolontha 325
Melosperma 30, 40, 186–187
Menispermaceae 184, 356
Menispermum 184
Mertensia 370
Mesembrianthemum 349
Metaplexis 36, 356
Meyna 334
397
BenSch et al.
Mikania 296, 321
Milium 321
Mimosa 370
Mimulus 34, 40, 187
Momordica 38, 100–101
Monarda 322
Monilinia 43, 115
Moraceae 92–93, 146, 205, 210, 308, 326, 346, 357, 364
Mortar 172
Morus 92, 210, 357, 364
mosses 345
Mundulea 337
Musa 146, 206, 210, 272, 301, 322 (also see banana)
Musaceae 146, 206, 210, 272, 301, 322
Musca 64, 340
Muscari 201, 369
mycorrhizal roots 144, 172
Mycosphaerella 184
Mycosphaerellaceae 93
Myristica 331
Myristicaceae 331
Myrothecium 227
Myrsine 356
Myrtaceae 28–29, 40, 52, 71, 74, 91–92, 117, 123, 188–189, 210,
227, 272, 297, 313, 323, 329, 334, 345, 361, 368
Myrtus 323
myxomycete 297
N
Narcissus 36, 169–170
Nartheciaceae 40, 201
Narthecium 31, 33, 40, 201, 203
Nectandra 199, 311
Nelumbo 95
Neocheiropteris 41, 358
Neottopteris 37, 358
Nerium 34, 36, 157, 190, 192, 356–357
Nicotiana 358, 364
Nigella 52
Nitraria 358
Nitrariaceae 358
nuts 145 (also see food)
Nyctaginaceae 40, 67
O
oats 210, 228, 364
Oberna 243–244
Ochrosia 368
Ocotea 32, 39, 199
Oidium 257, 296, 327
ointment 300
Olea 323, 339
Oleaceae 40, 48, 92, 125, 157, 265, 312–313, 315, 323, 337, 339,
344, 346, 362, 364
olive fruit waste 311
Onagraceae 40, 245, 340, 343
Onchorhyncus 338
Oncidium 33, 40, 196, 198, 205
Oncoba 35, 41, 56, 93, 195–196, 210
Onobrychis 369
398
Onosma 64
Onychiurus 253
Ophiopogon 37, 311, 358
Oplismenus 359
Opuntia 361
Orchidaceae 40, 196, 198, 205, 232, 257, 272, 305, 324
Orchis 324
Oreodaphne 199
Ornithogalum 31, 37, 39, 200–201
Oryza 129, 307, 308, 357, 359
Osmanthus 339
Ostericum 324
Ourisia 52
P
Pachylaena 305
Padus 313
Paeonia 4, 23, 93, 227, 306, 324
Paeoniaceae 93, 227, 306, 324
Paint 24, 157, 253–354
painted surface 101, 354
Pandanaceae 169
Pandanus 169
Panicum 257, 326
Papaver 275–276, 369
Papaveraceae 310, 315, 336, 342, 369
paper 302, 351, 369, 375
Paphiopedilium 199
Pardanthopsis 168
Parmelia 43, 175
Parodiopsidaceae 300
Pasania 354
Passilora 32, 40, 185–186, 203
Passiloraceae 40, 185,–186, 203, 336
Paullinia 325
pawpaw 92
peat 253
pecan tree 210
Peltigera 175, 319
Peltigeraceae 319
Penstemon 272
Pericallis 64
Peridermium 135, 180
Peronospora 261, 275–276
Peronosporales 275
Persea 199
Persica 215
Persicaria 309
Pertusaria 43, 62, 173, 175
Petasites 48
Peucedanum 324
Peucedanum 324
Phaenocoma 37, 210–211
Phaeophyscia 43, 173, 175
Phalaris 157, 227, 348, 369
Phaseolus 27, 33, 39, 128, 172–173, 326, 349–350, 360
Phleum 31, 41, 211, 213, 352, 360
Phoenix 184
Pholiota 157
Photinia 32, 41, 106–107, 130
Phragmidium 92, 275–276
the genuS Cladosporium
Phragmipedium 199
Phragmites 72, 324, 326, 369
Phrymaceae 40, 187
Phyllachora 326
Phyllachoraceae 326
Phyllactinia 17, 29, 43, 64, 92, 119–120, 128, 131, 165, 180, 185,
210, 213, 215, 229, 257, 269, 275–276
Phyllanthaceae 347
phylloplane 1, 157
Phyllostachys 357
Physcia 43, 173, 175
Physconia 175
Phytolacca 92, 309, 336
Phytolaccaceae 40, 92, 236, 238, 309, 336
Picea 172, 193, 241, 291, 329, 340, 358
Pinaceae 40, 64, 88–89, 93, 134, 157, 193, 218, 227, 241, 264, 291,
296, 310, 328–329, 331, 340, 354–355, 358, 362–363, 369
Pinus 40, 64, 88–89, 93, 134, 193, 218, 220, 227, 264, 296, 310,
329, 331, 340, 354–355, 362–363
Piper 34, 40, 220–221
Piperaceae 40, 220
Piptoporus 180
Pisum 35, 39, 92–93, 221, 290, 350, 360–361
Pithecellobium 297, 326
Pithecolobium 366
Plantaginaceae 40, 52, 186, 272, 345
Platycodon 38, 257, 360
Plectranthus 92, 235
Pleochaeta 275–276
Pleurotus 366
plum 218
Plumbaginaceae 48
Poa 312, 314, 331, 348
Poaceae 41, 48, 50, 52, 57, 64, 72–73, 75, 93, 95, 114, 152,
157, 163, 165, 184, 205, 211, 227–228, 245, 253, 256–257,
269, 284, 302, 306–308, 310, 312, 314–315, 321, 323–324,
326–327, 331, 335–337, 340, 343, 346, 348, 351–353, 357,
359–360, 365, 367–369
Podocarpaceae 205
pole , Douglas-ir 52
pole , Southern pine 228
Polygonaceae 309
Polygonatum 30, 37, 39, 48, 52, 222, 360, 368
Polygonum 48, 309
Polypodiaceae 41, 358
Polypogon 57
Polyporaceae 52, 157
Polyporales 44, 313
Polyporus 52, 155, 157, 180, 182, 313
Polytrichaceae 360
Polytrichum 360
Populus 33–35, 41, 64, 152, 175, 184, 222–223, 272, 275, 299,
318, 320–321, 329, 332
powdery mildew 43, 128, 131, 215, 275–276
Primula 41, 73–74
Primulaceae 41, 73, 247, 308, 320, 356
Printzia 318
Prosthechea 232–233
Protea 70, 83, 210, 217
Proteaceae 41, 57, 70, 83, 142, 210, 217
Prunus 93, 115–116, 157, 180, 206, 215, 217, 293, 297, 304–305,
313, 317, 325, 359, 361
www.studiesinmycology.org
Psamma 327
Pseudognaphalium 272
Psidium 95, 361
Psoralea 36, 39, 228, 290, 350
Pteridaceae 296
Pteridium 227, 340, 348
Pteris 348
Pteroscypsela 317
Puccinia 48, 93, 106, 165, 274–276, 291
Pucciniaceae 93, 274
Pucciniales 44, 275
Pucciniastraceae 227
Pucciniastrum 43–44, 134–135
Pulsatilla 275
Puya 180
Pyrus 64, 275–276, 308, 311, 320, 327, 362
Q
Quercus 35, 39, 52, 73, 127–128, 175, 184, 267, 275–276, 298,
302–303, 308, 312, 317, 343, 356, 365
R
railroad ties 192
Ranunculaceae 41, 48, 52, 122, 141, 157, 364, 370
Ranunculus 30, 35, 41, 122–123, 141–142
Raphanus 327, 361
resin 4, 23–24, 300, 329
Rhamnaceae 157, 335, 367
Rhamnus 152, 157
Rhododendron 32–33, 38, 235, 329, 357
Rhodomyrtus 329
Rhus 36, 286, 288, 298, 323, 329, 351
Rhytismetaceae 355
Ribes 48, 308, 312
Ricinus 92, 193, 344, 362
Rivina 34, 40, 236, 238
Robinia 31, 38, 193, 238
rock 144, 272, 300
Rohdea 323
Rosa 147, 206, 228, 272, 283, 312, 313
Rosaceae 41, 64, 92–93, 106, 115–116, 130, 138, 157, 180, 215,
217, 227–228, 241, 272, 283, 293, 297, 304–305, 308–313,
315, 317, 320, 323, 325, 327, 345, 358, 361–362, 365
Rubiaceae 41, 132, 306, 315, 323, 334, 360
Rubus 92, 138, 241, 275–276, 313, 315
Rumex 48
Ruscus 111, 242–243
Russula 157
Russulaceae 157
Russulales 44,
rust fungi 42–44, 48, 114, 128, 134, 138–139, 272, 274–276, 291
Ruta 33, 41, 239
Rutaceae 41, 64, 97, 146, 157, 165, 239, 250, 272, 303, 307, 310,
331, 342–343, 346–347, 364, 367, 369
rye 245
S
Saccharum 275–276, 310, 353
Sagittaria 73, 227, 316
399
BenSch et al.
Saissetia 353
Salicaceae 41, 64, 93, 157, 184, 187, 195, 210, 222, 272, 293,
299, 312, 318, 320–321, 329, 330, 332
Salix 33, 41, 48, 64, 157, 187–188, 275, 293, 312, 330
salterns 17, 52, 110, 131, 144–146, 149, 184, 229–230, 239, 241,
253–254, 256, 258, 264, 269, 286
Sambucus 93, 165, 193, 310, 330, 337, 362
Sanicula 328
Sapindaceae 41, 70, 72, 307, 316, 325, 352, 353
Saponaria 114
Sapotaceae 41, 87
Sarrameanaceae 319
Sasa 306
sausage 343, 362 (also see food)
Saxifraga 122
Schoenoplectus 106
Scilla 330
Scirpus 106, 315
Scleroderma 180
Sclerotinia 43, 115–116
Sclerotiniaceae 115–116
Scorzonera 48
Scrophulariaceae 42, 64, 187, 352
seaweed 106
Secale 245, 337, 351
Sechium 38, 100–101, 316
sediment 272
Selaginella 337
Selaginellaceae 337
Senna 39, 81, 83, 326
Sesbania 106
Sesleria 52
Setaria 257
Shorea 272
Sida 331
Silene 38, 48, 114, 243–244
silicone 229, 253
Simaroubaceae 344
Sisyrinchium 368
Smilacaceae 42, 245, 330, 362
Smilax 32–33, 42, 245, 247, 330
soil 1, 24, 91–93, 157, 205, 227, 253, 272, 306, 309, 338, 342, 359
Solanaceae 101, 274, 296, 304, 312, 331, 347, 349, 355, 358, 364
Solanum 101, 195, 296, 312, 331, 347, 355
Soldanella 33, 41, 247–248, 250
Sonchus 48, 64
Sorbus 323
Sorghum 157, 205, 228, 290, 315
Sphaeria 310
Spinacia 31, 38, 93, 133, 180, 277, 281
Spiraea 366
Sporobolus 323, 336
Stanhopea 33, 40, 257–258, 272
Stellaria 92
Sterculia 343
Stereum 362
Stipa 331
stones 351
straw 341, 347, 363
strawberry 92
Strelitzia 135–136, 210, 272
Strelitziaceae 135, 210, 272
400
Strobilanthes 36, 363
Strophariaceae 157
Stylosanthes 362
Styracaceae 42
Styracaceae 42, 162
Styrax 34, 42, 162
Suillus 116, 157, 180
Sweetia 311
Swida 308
Swietenia 65
Symphoricarpos 332, 370
Symphytum 62, 64
Syringa 31, 40, 175, 265–267, 313, 337, 364
Syzygium 323
T
Taphrina 29, 43–44, 215, 217–218, 267–268, 349
Taphrinaceae 217
Taphrinales 44, 267
Tectona 332–333
Teloschistaceae 60
Teratosphaeria 43, 83, 85, 210, 217
Teratosphaeriaceae 83, 210, 217
Tetrapanax 36, 365
Teucrium 39, 48, 365
Textile 93, 157, 253, 342
Thalictrum 48, 370
Thamnolia 175
thatch 146
Thelypodium 38, 79
Theobroma 365
Thouinia 353
Thuja tincture 52
Thymelaeaceae 42, 335, 338, 345
Tilia 52, 106, 165, 334, 347
Tiliaceae 52, 106, 165, 347, 356
Tragopogon 33, 37, 190, 195, 223–224
Tranzschelia 48, 275–276
Trillium 31–32, 39, 272, 274
Triosteum 334
Triphragmium 275
Triticum 93, 163, 165, 184, 228, 245, 275–276, 351, 369
Trollius 48
Tropaeolaceae 338
Tropaeolum 338
Tubercularia 359
Turritis 261–262
Tussilago 48
Typha 27, 42, 72–73, 146–147, 158, 160, 264, 366
Typhaceae 42, 72, 146, 158, 366
U
Ulmaceae 42, 160–161, 205, 283, 313
Ulmus 34, 42, 160–161, 205, 283, 313
Uredinales 44, 134, 138, 276, 315, 366
Uredo 83, 366
Uromyces 48
Urtica 315
Urticaceae 315
the genuS Cladosporium
V
Vaccinium 120–121, 324
Vachellia 72
Valeriana 92
Valerianaceae 92
Vangueria 334
Venturiaceae 293
Verbenaceae 318, 333
Vernonia 227
Viburnum 353, 370
Vigna 36, 39, 92, 128–129, 139–140, 288, 290
Vinca 33, 36, 157, 275, 291
Vincetoxicum 301
Viola 92, 275
Violaceae 92
Viscaria 114
Vitaceae 233, 297, 313, 326–328, 330, 335, 341, 355, 366–367
Vitis 233, 297, 313, 326–328, 330, 335, 341, 355, 366–367 (also
see grape)
water, industrial 52
wheat 92–93, 210, 228, 245, 376
Wikstroemia 335, 338
window frame 144, 172
wine vault 305
wood 22–24, 79, 93, 144, 157, 172, 184, 193, 205, 217, 228, 241–
242, 253, 272, 291–293, 299–300, 303, 310, 315, 319, 322,
323, 327, 337, 340, 342–343, 351, 357, 362, 364–365, 374
wort 307
X
Xanthoria 43, 175
Xanthorrhoeaceae 42, 54, 168, 185, 228, 336, 344
Xyridaceae 42, 293–294
Xyris 35, 42, 293–294
Y
Yucca 33, 37, 73, 295
Yulanea 184
W
Z
wall 322
wall plaster 313
wallpaper 302
water 52, 145, 184, 227, 272
water, hypersaline 17, 52, 108, 110, 131, 144, 146, 149, 152,
184, 229–230, 232, 239, 241, 253–254, 256, 258, 260, 264,
268–269, 285, 286
www.studiesinmycology.org
Zea 64, 73, 183, 269, 335, 348, 365, 367
Zingiber 339
Zingiberaceae 42, 339
Zinnia 25, 30, 37, 194
Ziziphus 367
Zygia 366
401