TAXON 60 (4) • August 2011: 941–952 Razafimandimbison & al. • A basal grade in the Vanguerieae alliance M O L E C U L A R PH Y LO G E N E T I C S A N D B I O G E O G R A PH Y Molecular support for a basal grade of morphologically distinct, monotypic genera in the species-rich Vanguerieae alliance (Rubiaceae, Ixoroideae): Its systematic and conservation implications Sylvain G. Razafimandimbison,1 Kent Kainulainen,1,2 Khoon M. Wong, 3 Katy Beaver4 & Birgitta Bremer1 1 Bergius Foundation, Royal Swedish Academy of Sciences and Botany Department, Stockholm University, 10691 Stockholm, Sweden 2 Department of Botany, Stockholm University, 10691, Stockholm, Sweden 3 Singapore Botanic Gardens, 1 Cluny Road, Singapore 259569 4 Plant Conservation Action Group, P.O. Box 392, Victoria, Mahé, Seychelles Author for correspondence: Sylvain G. Razafimandimbison, sylvain.razafimandimbison@bergianska.se Abstract Many monotypic genera with unique apomorphic characters have been difficult to place in the morphology-based classifications of the coffee family (Rubiaceae). We rigorously assessed the subfamilial phylogenetic position and generic status of three enigmatic genera, the Seychellois Glionnetia, the Southeast Asian Jackiopsis, and the Chinese Trailliaedoxa within Rubiaceae, using sequence data of four plastid markers (ndhF, rbcL, rps16, trnT­F). The present study provides molecular phylogenetic support for positions of these genera in the subfamily Ixoroideae, and reveals the presence of a basal grade of morphologically distinct, monotypic genera (Crossopteryx, Jackiopsis, Scyphiphora, Trailliaedoxa, and Glionnetia, respectively) in the species-rich Vanguerieae alliance. These five genera may represent sole representatives of their respective lineages and therefore may carry unique genetic information. Their conservation status was assessed, applying the criteria set in IUCN Red List Categories. We consider Glionnetia and Jackiopsis Endangered. Scyphiphora is recognized as Near Threatened despite its extensive range and Crossopteryx as Least Concern. Trailliaedoxa is poorly known (Data Deficient). Finally, the generic status of Glionnetia, Jackiopsis, and Trailliaedoxa and the monogeneric tribe Jackieae as defined by Ridsdale are supported. Keywords conservation; Crossopteryx; Glionnetia; Jackiopsis; molecular systematics; Scyphiphora; Trailliaedoxa INTRODUCTION Rubiaceae (or the coffee family) are one of the most species-rich flowering plant families with a particularly high number of monotypic genera, which represent 34.5% of the genera (Davis & al., 2009). The majority of these genera have been studied in previous molecular phylogenetic investigations of the family, and their generic status, systematic positions, and phylogenetic relationships have been assessed. Some monotypic genera have been synonymized, as they were shown to be part of much larger and well-defined genera (e.g., Aphaenandra Miq. now included in Mussaenda L., Alejandro & al., 2005; Hymenocnemis Hook. f. now included in Gaertnera Lam., Malcomber & Davis, 2005; Litosanthes Blume now included in Lasianthus Jack, Xiao & Xhu, 2008; Rhopalobrachium Schltr. & K. Krause now included in Cyclo­ phyllum Hook. f., Mouly & al., 2007; Schizostigma Arn. ex Meisn. now included in Sabicea Aubl., Khan & al., 2008; Neoleroya Cavaco and Scyphochlamys Balf. f. now included in Pyrostria Comm. ex A. Juss., Razafimandimbison & al., 2009). In contrast, the generic status of some monotypic genera (e.g., Strumpfia Jacq., Bremer & al., 1995; Crossopteryx (Afzel. ex G. Don) Benth., Razafimandimbison & Bremer, 2001; Burttdavya Hoyle, Razafimandimbison & Bremer, 2002; Landiopsis Capuron ex Bosser, Alejandro & al., 2005; Dunnia Tutcher, Rydin & al., 2008) has been retained, because they are not nested within well-defined genera. Furthermore, they have at least one autapomorphic character allowing them to be easily identified (Backlund & Bremer, 1998). The systematic placement of a few rubiaceous monotypic genera with particularly unique morphology remains either unknown or controversial due to the lack of sequenceable material. The present study focuses on the enigmatic genera Trailliaedoxa W.W. Sm. & Forrest, Jackiopsis Ridsdale, and Glionnetia Tirvengadum (Fig. 1A–J). Jackiopsis was originally described by Wallich (in Roxburgh, 1824) as Jackia Wall., but this latter name was already used for another genus Jackia Blume in the family Polygalaceae. As a result, Ridsdale (1979) proposed the name Jackiopsis for Wallich’s intended genus. When establishing Jackia ornata Wall. (= Jackiopsis ornata (Wall.) Ridsdale) Wallich (in Roxburgh, 1824) wrote a very detailed description based on individual plants in flower and immature fruit that he had discovered “on several of the small islands in the immediate vicinity of Singapore”. Jackiopsis is a large tree up to 35 m tall with spreading buttresses. The stipules are large, persistent, and connate into a sheath with 8 to 15 stiff, filiform lobes (Fig. 1D). Its inflorescences are axillary, large, pendulous, and paniculate with scorpioid lateral branches. The peduncles are typically flattened, articulated, and are composed of a primary peduncle unit, a secondary node, a secondary peduncle unit, a tertiary node, and a tertiary peduncle unit. Each secondary node bears a sheathed bract, and a pair of reduced leaves; each tertiary node bears a sheathed 941 Razafimandimbison & al. • A basal grade in the Vanguerieae alliance bract and is typically terminated by up to eight scorpioid inflorescences in umbel-like arrangement. Corolla lobe aestivation is valvate. Ovaries are bilocular with 2–5 ovules in each locule; the ovules are basally attached on ascending, stalked placentae. Fruits are dry, dehiscent, winged nutlets with persistent, reticulate, and accrescent calyx lobes (pterophylls) (Fig. 1E), commonly with only one seed per locule, as the remaining ovules are aborted (Wallich in Roxburgh, 1824; Ridsdale, 1979). Korthals (1851) was the first to classify Jackiopsis (as Jackia) at tribal level. He placed the genus in his new tribe TAXON 60 (4) • August 2011: 941–952 Jackieae (as Jackiae) along with four other genera, Tricaly­ sia A. Rich. and Diplospora DC. (now in tribe Coffeeae), Hypobathrum Blume (now in Octotropideae), and Scyphiphora Gaertn., all presently placed in the subfamily Ixoroideae. Hooker (1873) erroneously considered the corolla aestivation of Jackiopsis to be contorted and its fruits to be drupes with 5 to 7 pyrenes, and accordingly placed the genus in Retiniphylleae, now in subfamily Ixoroideae. Baillon (1878) postulated a close affinity between Jackiopsis, Cruckshanksia Hook. & Arn., and Carphalea Juss. based on similarities in ovary structure, placentation, and fruits with reticulate pterophylls. Fig. 1. Illustrations showing the morphological synapomorphies or characteristics of Trailliaedoxa, Jackiopsis, and Glionnetia. A–C, Trailliae­ doxa gracilis: A, leafy twig; from Boufford & al. 35041 (MO); B, immature fruit; from Yü 1348 (HUH); C, flower showing partly exserted anthers and a fully exserted stigma; from Forrest 12638 (S). D–F, Jackiopsis ornata: D, large, densely pubescent stipule with filiform lobes; from Kalat & al. s.n. 1993-05-11 (L); E, dry, indehiscent fruit with three unequal lobes; from Van Balooy 6109 (MO); F, densely pubescent flower with three calyx lobes and a very long style; from Rahmat Si Toroes 3424 (S). G–J, Glionnetia sericea: G, habit showing a very long, convolute terminal vegetative bud (young stipule); from Beaver 03 (S); H, long, convolute stipule; from Beaver 03 (S); I, mature capsular fruit; from Beaver 17 (S); J, flower showing partly exserted anthers; from Beaver 17 (S). Scale bars: thin, 2 mm; medium, 5 mm; thick, 20 mm. 942 TAXON 60 (4) • August 2011: 941–952 The latter two genera are currently placed in the tribes Coussareeae and Knoxieae, respectively, in the subfamily Rubioideae. Schumann (1891) associated Jackiopsis with Rubioideae, viz. Oldenlandieae. More recently, Ridsdale (1979) narrowly recircumscribed Jackieae to include only the genus Jackiopsis and tentatively placed the tribe in the subfamily Cinchonoideae. Finally, the recent discovery of intra-ovarian trichomes in Jackiopsis (Puff & Igersheim, 1994) and in some Malagasy species of Tricalysia (Ranarivelo-Randriamboavonjy & al., 2007) could be taken as an indication of a close relationship between the two genera and would imply placing the former in the tribe Coffeeae of Ixoroideae. Trailliaedoxa is an ericoid, erect subshrub (up to 60 cm tall), with a broom-like branching and small, tough leaves (Robbrecht, 1988). According to the protologue (Smith, 1917), the genus grows in the rocky habitats and thickets on dry mountain slopes of Sichuan, China, between 1450 and 3000 m elevation. Trailliaedoxa is very distinct from other Rubiaceae in its ericoid habit, pubescent styles, and schizocarpous fruits. The genus is presently unclassified at tribal level in Rubiaceae (Robbrecht, 1988). Smith (1917) thought that the floral structure of the genus is similar to that of Alberteae, now in Ixoroideae. On the other hand, its ericoid habit is also comparable to that of Spermadictyon Roxb. of the tribe Paederieae in the subfamily Rubioideae (Smith, 1917). Robbrecht (1988) tentatively placed Trailliaedoxa in the subfamily Antirheoideae, which was shown to be highly polyphyletic (e.g., Bremer & al., 1995). Finally, the genus Glionnetia was originally described by Tirvengadum (1984) to accommodate the Seychellois Randia sericea (Baker) Hemsl. (Hemsley, 1916: 18) that he excluded from the Neotropical genus Randia L. This monotypic genus is restricted to Mahé and Silhouette Islands of the Seychelles Archipelago; it is distinct by its large, long, intrapetiolar, and convolute stipules and can additionally be characterized by a combination of the following features: glabrous, coriaceous leaves clustered at the apex of the branches, corollas 6–7 cm long, left-contorted aestivation, and capsular fruits with loculicidal dehiscence (Fig. 1G–J). Tirvengadum (1984) originally classified Glionnetia in Rondeletieae of the subfamily Cinchonoideae. Robbrecht (1988), and more recently Rova & al. (2009) and Manns & Bremer (2010), tentatively followed this classification. None of the discussed hypotheses regarding systematic positions within Rubiaceae have been tested with molecular data. We rigorously investigated the phylogenetic positions of Glionnetia, Jackiopsis, and Trailliaedoxa in Rubiaceae and assessed their generic status, using sequence data from four plastid markers (ndhF, rbcL, rps16 intron, trnT­F). We found that these genera belong in the subfamily Ixoroideae, and that Crossopteryx, Jackiopsis, Scyphiphora, Trailliaedoxa, and Glionnetia, respectively, form a paraphyletic assemblage at the base of the species-rich Vanguerieae alliance. These results allowed us to hypothesize that these monotypic genera may represent sole representatives of their respective lineages. We discuss the systematic and conservation implications of these findings. Razafimandimbison & al. • A basal grade in the Vanguerieae alliance MATERIALS AND METHODS Taxon sampling and laboratory work. — We extracted silica-gel leaf samples from three individuals of Jackiopsis ornata (from the Malay Peninsula), three individuals of Glion­ netia sericea (from the Island of Mahé in the Seychelles Archipelago), and two individuals of Trailliaedoxa gracilis (from Yunnan, China), following the protocol outlined in Razafimandimbison & al. (2008). The extracted DNA was amplified and sequenced for four chloroplast regions (ndhF, rbcL, rps16 intron, trnT­F), using the primers of Razafimandimbison & al. (2008) and Rydin & al. (2008). Phylogenetic analyses. — Sequences were preliminary aligned using the Clustal W (default settings; Thompson & al., 1994), as implemented in BioEdit v.7.0.9 (Hall, 1999), and subsequently edited manually. We initially performed maximum parsimony (MP) and Bayesian analyses of 120 rbcL sequences of Rubiaceae (including all major lineages in the three subfamilies of Rubiaceae and the tribes Luculieae and Coptosapelteae, which do not belong in any of the three subfamilies) in order to pinpoint the subfamilial position(s) of the three genera within the family. Eight taxa from the other families of the order Gentianales (Apocynaceae, Gentianaceae, Gelsemiaceae, Loganiaceae) were utilized as outgroups following Bremer & Eriksson (2009). Bayesian analysis of the rbcL dataset was performed using the GTR + G + I model. We refer to these MP and Bayesian rbcL analyses hereafter as the “Family-Wide-rbcL” analyses. The results of the Family-Wide-rbcL analyses allowed us to narrow down our sampling to include only the three targeted genera and their more closely related groups and select the outgroup taxa from within Rubiaceae (Cinchona L. and Cubanola Aiello [subfamily Cinchonoideae], Morinda L. [subfamily Rubioideae], and Luculia Sweet [Luculieae]). MP and Bayesian analyses of 71 taxa based on combined sequence data from the chloroplast markers ndhF, rbcL, rps16 intron, and trnT­F were subsequently performed, of which 67 species represented all tribes currently recognized in Ixoroideae (with the exception of Henriquezieae) (see Appendix). We refer to these analyses hereafter as the “Ixoroideae-Four-Locus” analyses. We hoped that we could better resolve the position of these genera within subfamily Ixoroideae, by combining the two more rapidly evolving non-coding regions (rps16 intron, trnT­F) and the two coding regions (ndhF, rbcL) in a single data matrix. Separate and combined MP analyses of the ndhF, rbcL, rps16, and trnT­F datasets of the 71 taxa were conducted using the program PAUP* v.4.0B10 (Swofford, 2002). MP analyses consisted of a heuristic search with the TBR branch swapping algorithm, Multrees on, 1000 random sequence addition replicates and a maximum of 10 trees saved per replicate. Clade bootstrap support (BS) was estimated using the same settings and three random sequence additions per replicate. The combined dataset was also analyzed using Bayesian inference (BI), using the program MrBayes v.3.1.2 (Huelsenbeck & Ronquist, 2001; Ronquist & Huelsenbeck, 2003). The combined data were partitioned in a protein-coding (rbcL, ndhF) partition and a non-coding (rps16 intron, trnT­F) partition; these were unlinked and analyzed under the GTR + G + I 943 Razafimandimbison & al. • A basal grade in the Vanguerieae alliance and GTR + G models, respectively, which were suggested as best-fit to the data under the corrected Akaike information criterion (AICc). The Bayesian rbcL analysis of the 120 taxa and combined Bayesian analyses of the 71 taxa each comprised two runs of four chains, which were monitored for 15 × 106 generations, with every 1000th generation being sampled, and the temperature coefficient of the chain-heating scheme set to 0.15. Stationarity and convergence of runs, as well as the correlation of split frequencies between the runs were checked using the TAXON 60 (4) • August 2011: 941–952 program AWTY (Nylander & al., 2008). Trees sampled before the posterior probability (PP) of splits stabilized, were excluded from consensus as a burn-in phase. The effective sample size (ESS) of parameters was checked using the program Tracer v.1.4.1 (Rambaut & Drummond, 2007). Finally, we assessed the conservation status of Crossop­ teryx, Glionnetia, Jackiopsis, Scyphiphora, and Trailliaedoxa, applying the criteria set in World Conservation Union (IUCN) Red List Categories (Version 3.1; IUCN, 2001). Alstonia Kopsia Gelsemium Mostuea Strychnos Spigelia Gentiana Anthocleista 1 1 1 1 1 100 0.53 outgroups Luculieae (3 spp.) 1 93 Coptosapelteae (5 spp.) 0.99 Subfamily Rubioideae (63 spp.) 1 99 1 100 Rubiaceae 1 96 Subfamily Cinchonoideae (11 spp.) 0.78 1 99 1 1 93 1 82 Subfamily Ixoroideae (30 spp.) 0.77 0.91 1 0.51 1 97 0.72 0.81 0.63 Fig. 2. A Bayesian majority rule consensus tree of the coffee family from the rbcL sequence data of 120 taxa. Posterior probabilities and bootstrap support percentages are above and below the nodes, respectively. The outgroup taxa are delimited by a vertical line. Numbers in parentheses correspond to the number of species sequenced. 944 0.87 1 86 1 100 0.53 0.72 1 71 1 0.96 0.86 61 55 0.93 1 0.84 67 Mussaenda erythrophylla Sipanea biflora Sipanea hispida Virectaria major Sabicea diversifolia Sabicea aspera Ferdinandusa speciosa Calycophyllum candidissimum Emmenopterys henryi Condaminea corymbosa Pyrostria hystrix Vangueria madagascariensis Retiniphyllum pilosum Ixora coccinea Glionnetia sericea 1 Glionnetia sericea 2 Glionnetia sericea 3 Crossopteryx febrifuga Trailliaedoxa gracilis 1 Trailliaedoxa gracilis 2 Alberta magna Jackiopsis ornata Razafimandimbisonia sambiranensis Aidia micrantha Bertiera guianensis Tricalysia cryptocalyx Coffea arabica Diplospora polysperma Cremaspora triflora Fernelia buxifolia Feretia aeruginescens Kraussia floribunda Pouchetia gilletii TAXON 60 (4) • August 2011: 941–952 Razafimandimbison & al. • A basal grade in the Vanguerieae alliance RESULTS Separate analyses. — The Family-Wide-rbcL analyses based on the 120 rbcL sequence data placed all sampled Glion­ netia, Jackiopsis, and Trailliaedoxa in the subfamily Ixoroideae (PP = 1.00; BS = 82%). The monophyly of Ixoroideae and its sister-group relationship with the subfamily Cinchonoideae is consistent with other published Rubiaceae studies (e.g., Robbrecht & Manen, 2006; Bremer & Eriksson, 2009; Rydin & al., 2009). Glionnetia and Trailliaedoxa formed a poorly supported clade with Crossopteryx, while Jackiopsis was left unresolved in the poorly supported Coffeeae alliance (PP = 0.53) (Fig. 2). A summary of the tree data and statistics from the analyses is given in Table 1. In the separate Ixoroideae analyses of the 71-taxa datasets, the incongruent nodes between the separate cpDNA markers generally received weak support (trees not shown), with the exception of a node inferred from the trnT­ F data: a clade consisting of Condamineeae, Sipaneeae and Posoquerieae, as sister to the rest of the Ixoroideae (BS = 81%; PP = 0.96) excluding Mussaendeae and Sabiceeae (inferred as sister groups in a basal clade). Combined analyses. — The phylogenetic hypotheses inferred from the MP and BI analyses of the Ixoroideae-FourLocus datasets were in general agreement, with the exception of the position of Tocoyena pittieri, resolved as sister to Gar­ denia hansemanii in the former (BS = 53%), and as sister to Aulacocalyx jasminiflora in the latter (PP = 0.89). The uncorrected potential scale reduction factor (PSRF; Gelman & Rubin, 1992) of the summarized parameters approached 1.0, and the split frequencies between the runs were highly correlated, indicating that the runs had converged. The ESS of all model parameters were > 200, as recommended by Drummond & al. (2007) for adequate representation of the posterior probability. The 50% majority-rule consensus tree inferred from the BI analysis of the Ixoroideae-Four-Locus data is shown in Fig. 3. The sampled specimens of Glionnetia, Jackiopsis, and Trailliaedoxa formed, respectively, highly supported clades (PP = 1.00; BS = 100) and were all nested in the highly supported Vanguerieae alliance (Fig. 3). Within this alliance the monotypic African genus Crossopteryx was the first to branch off, followed by Jackiopsis, Scyphiphora, Trailliaedoxa, and Glionnetia, respectively. Glionnetia was in turn resolved with poor support as sister to a poorly supported large clade containing Vanguerieae, Greeneeae, Aleisanthieae, and Ixoreae. Within this latter clade Aleisanthieae and Ixoreae formed a sister group, which in turn was sister to Greeneeae. The (Greeneeae(AleisanthieaeIxoreae)) clade was sister to Vanguerieae. DISCUSSION Subfamilial position of Glionnetia, Jackiopsis and Trailliaedoxa within Rubiaceae: New circumscription of the Vanguerieae alliance. — The present study is the first molecular phylogenetic investigation of the Seychellois genus Glion­ netia, the Southeast Asian Jackiopsis, and the Chinese genus Trailliaedoxa. The results of the Family-Wide-rbcL analysis (Fig. 2) support the placement of these monotypic genera in the subfamily Ixoroideae sensu Bremer & Eriksson (2009). The phylogenetic hypothesis from the Ixoroideae-Four-Locus analyses (Fig. 3) reveals that the Vanguerieae alliance of the subfamily Ixoroideae contains the following major lineages: the African Crossopteryx (Crossopterygeae), Jackiopsis (Jackieae sensu Ridsdale), the mangrove genus Scyphiphora, Trailliae­ doxa, Glionnetia, all currently unplaced at tribal level, and the tribes Vanguerieae, Aleisanthieae, Greeneeae, and Ixoreae. This alliance is species-rich and morphologically diverse, and contains over 1100 species of shrubs and small trees; it does not seem to have any obvious synapomorphic character, however the majority of its members (i.e., those of the species-rich tribes Ixoreae and Vanguerieae) have flowers showing secondary pollen presentation, drupaceous fruits, and one-seeded carpels. Ixoreae is pantropical, while the remaining lineages are paleotropical. An overview of the morphological and ecological characteristics of these major lineages of the Vanguerieae alliance is shown in Table 2. Tribal positions and generic status of Glionnetia, Jackiopsis and Trailliaedoxa. — The morphologically unique genera Glionnetia, Jackiopsis, and Trailliaedoxa represent a paraphyletic basal assemblage within the Vanguerieae alliance (Fig. 3). Assessment and recognition of these monotypic genera and their tribal positions are discussed on the basis of a combination of the following criteria (Backlund & Bremer, 1998; Razafimandimbison & Bremer, 2002): (1) if they are not nested within other well-defined genera; and (2) if they have at least one Table 1. Characteristics of the markers/datasets used, including statistics of alignments, analyses, as well as parameter estimates and evolutionary models suggested by MrModeltest 2.2 (Nylander, 2004). ndhF rbcL rps16 trnT­F Combined Coding Noncoding Number of taxa 40 53 65 66 66 56 66 Included characters 2159 1403 1073 2441 7076 3562 3514 Variable characters 677 291 382 886 2236 968 1268 Parsimony-informative characters 353 158 227 465 1203 511 692 % informative characters 16.35 11.26 21.16 19.05 17.00 14.35 19.69 Consistency index (CI) 0.70 0.54 0.68 0.75 0.68 0.64 0.72 Retention index (RI) 0.73 0.64 0.82 0.82 0.77 0.69 0.82 945 Razafimandimbison & al. • A basal grade in the Vanguerieae alliance TAXON 60 (4) • August 2011: 941–952 Luculia gratissima - LUC Morinda citrifolia - MOR 1.00 100 1.00 92 1.00 94 1.00 100 0.90 66 1.00 94 1.00 100 0.80 65 0.98 87 Subfamily Ixoroideae Cinchona pubescens - CIN Cubanola domigensis - CHI Posoqueria latifolia - POS Sipanea biflora - SIP Sipanea hispida - SIP 1.00 100 1.00 Sipanea pratensis - SIP 100 Emmenopterys henryi - CON Calycophyllum candidissimum - CON Pentagonia macrophylla - CON Rustia thibaudioides - CON Mussaenda arcuata - MUS 1.00 Pseudomussaenda flava - MUS 100 Sabicea villosa - SAB 1.00 Tamridea capsulifera - SAB 100 Steenisia pleurocarpa 1.00 68 Retiniphyllum concolor - RET Retiniphyllum maguirei - RET Retiniphyllum pilosum - RET 0.99 91 Retiniphyllum schomburgki - RET Airosperma vanuense Boholia nematostylis 1.00 100 1.00 100 1.00 100 Augusta rivalis Wendlandia tinctoria Alberta magna - ALB 1.00 1.00 Nematostylis anthophylla - ALB 100 100 Razafimandimbisonia minor - ALB 1.00 1.00 1.00 Razafimandimbisonia sambiranensis - ALB 99 93 100 Bertiera guianensis - BER 0.98 Diplospora polysperma - COF 1.00 79 100 Coffea arabica - COF 1.00 87 Tricalysia cryptocalyx - COF 0.87 52 1.00 Cremaspora triflora subsp. confluens - CRE 100 1.00 1.00 Hypobathrum racemosum - OCT 100 100 Aulacocalyx jasminiflora - GAR 0.99 83 Tocoyena pittieri - GAR 1.00 Gardenia hansemanii - GAR 0.89 75 Pavetta abyssinca - PAV 0.69 Coffeeae alliance 1.00 Tennantia sennii - PAV 100 Crossopteryx febrifuga 1 - CRO 1.00 Crossopteryx febrifuga 2 - CRO 100 Jackiopsis ornata 1 - JAC Jackiopsis ornata 2 - JAC 1.00 1.00 100 90 Jackiopsis ornata 3 - JAC Scyphiphora hydrophylacea 1 1.00 1.00 Scyphiphora hydrophylacea 2 100 93 Trailliaedoxa gracilis 1 1.00 Trailliaedoxa gracilis 2 100 1.00 67 Glionnetia sericea 1 Glionnetia sericea 2 1.00 100 Glionnetia sericea 3 0.98 59 Vanguerieae alliance Canthium tetraphyllum - VAN 1.00 Vangueria madagascariensis - VAN 100 1.00 Keetia guenzii - VAN 0.55 100 Cyclophyllum deplanchei - VAN 1.00 100 Peponidium comorense - VAN 0.99 75 Pyrostria hystrix - VAN 0.98 65 0.53 Greenea sp. 1 - GRE Greenea oblonga - GRE 1.00 100 Greenea corymbosa - GRE 1.00 86 Greenea sp. 2 - GRE 0.86 70 1.00 Aleisanthia rupestris - ALE 100 1.00 Aleisanthiopsis distantiflora - ALE 99 Ixora coccinea - IXO 1.00 Ixora margaretae - IXO 98 1.00 1.00 90 Ixora moorensis - IXO 99 1.00 Ixora trilocularis - IXO 100 Ixora brachypoda - IXO 0.93 67 Ixora guianensis - IXO 1.00 991.00 Ixora hookeri - IXO 1.00 - 0.01 outgroup taxa 1.00 100 93 Fig. 3. Bayesian majority-rule consensus tree of the subfamily Ixoroideae from the combined ndhF/rbcL/rps16/trnL­F data. Below branches are posterior probabilities and bootstrap support percentages (bold). Bootstrap support < 50% is indicated by a dash. The outgroup taxa are delimited by a vertical line. ALB, Alberteae; ALE, Aleisanthieae; CHI, Chiococceae; CIN, Cinchoneae; COF, Coffeeae; CON, Condamineeae; CRE, Cremasporeae; CRO, Crossopterygeae; GAR, Gardenieae; GRE, Greeneeae; IXO, Ixoreae; JAC, Jackieae; LUC, Luculieae; MUS, Mussaendeae; OCT, Octotropideae; PAV, Pavetteae; POS, Posoquerieae; RET, Retiniphylleae; SAB, Sabiceeae; SIP, Sipaneeae; VAN, Vanguerieae. The positions of the subfamily Ixoroideae and the Coffeeae and Vanguerieae alliances are indicated. The sampled specimens of Glionnetia, Jackiopsis, and Trailliaedoxa are in boldface. 946 TAXON 60 (4) • August 2011: 941–952 Razafimandimbison & al. • A basal grade in the Vanguerieae alliance autapomorphic character or a combination of plesiomorphic characters, allowing them to be recognized easily. Glionnetia, Jackiopsis or Trailliaedoxa are not nested within the five currently recognized tribes (Aleisanthieae, Crossopterygeae, Greeneeae, Ixoreae, Vanguerieae) of the Vanguerieae alliance. Like the monogeneric tribe Crossopterygeae, each of these three genera can also be placed in their own tribes, as they have distinct morphological features that warrant tribal recognition. The large, persistent, and sheathed stipules with 8–15 filiform lobes and the large (Fig. 1D), paniculate inflorescences with scorpioid lateral branches are unique for Jackiopsis in the Vanguerieae alliance and may represent potential apomorphies for the genus. A combination of valvate aestivation and dry, indehiscent fruits with pterophylls (Fig. 1E) can additionally be used to characterize Jackiopsis. Within the Vanguerieae alliance the members of Vanguerieae also have valvate aestivation but their fruits are drupes (Table 2). Dry fruits with pterophylls are also found in some other rubiaceous genera (e.g., Steenisia Bahk. f., Bremer, 1984; Cosmocalyx Standl., Delprete, 1998; Alberta E. Mey, Kainulainen & al., 2009). Furthermore, the Ixoroideae-Four-Locus analyses (Fig. 3) support the monogeneric tribe Jackieae as circumscribed by Ridsdale (1979). Consequently, an older view of Jackieae (Korthals, 1851), as containing Diplospora, Hypobathrum, Jackiopsis, Scyphiphora, and Tricalysia, is highly polyphyletic. The phylogenetic positions of Diplospora and Tricalysia in Coffeeae and Hypobathrum in Octotropideae have been shown by many independent molecular phylogenetic studies (e.g., Andreasen & Bremer, 2000; Robbrecht & Manen, 2006). The fourth genus Scyphiphora has been shown to be closely related to Aleisanthieae, Greeneeae, Ixoreae, and Vanguerieae of the Vanguerieae alliance (e.g., Robbrecht & Manen, 2006; Bremer & Eriksson, 2009; Cortés-B. & al., 2009; Mouly & al., 2009). A close relationship of Jackiopsis with the former Hedyotideae (now Knoxieae in subfamily Rubioideae) as put forward by Baillon (1878) and Schumann (1891) is not supported by this study (Fig. 2). The Ixoroideae-Four-Locus analyses (Fig. 3) strongly support the position of Glionnetia in the Vanguerieae alliance, although its relationship to other taxa within the alliance is poorly resolved. This finding is inconsistent with Tirvengadum (1984), who tentatively placed the genus in Rondeletieae of the subfamily Cinchonoideae. Within Rondeletieae, Tirvengadum (1984) postulated a close relationship to the genus Lindenia Benth. (now Augusta Pohl), a genus that has recently been demonstrated to have a close affinity with the genus Wend­ landia Bartl. ex DC. (Rova & al., 2002) of the Coffeeae alliance of Ixoroideae (Fig. 3). The long, intrapetiolar, and convolute stipules are unique for Glionnetia within the Vanguerieae alliance, and may represent a potential apomorphy for the Table 2. Morphological characteristics and other important information of the major lineages of the Vanguerieae alliance. Crossopterygeae Jackieae Scyphi­ phora Traillaiae­ doxa Vanguerieae Aleisanthieae Greeneeae Ixoreae Distribution Tropical Africa SE Asia SE Asia and Madagascar Yunnan, Sichuan, China Seychelles Paleotropics SE Asia SE Asia Pantropical Habit Shrubs to small trees Tall to emergent trees Shrubs Subshrubs (up to 60 cm tall) Small trees Small trees, Shrubs shrubs or herbs Shrubs or small trees Shrubs Inflorescence position Terminal Axillary (rarely axillary/ terminal) Axillary Terminal/ axillary Terminal Axillary Terminal/ axillary Terminal Terminal/ cauliflorous Inflorescence type Paniculate Paniculate with scorpioid lateral branches Cymose Cymose to umbelliform or fasciculate Paniculate Cymose Scorpioid Scorpioid Cymose/ paniculate Corolla aestivation Leftcontorted Valvate Leftcontorted Leftcontorted Leftcontorted Valvate Leftcontorted Leftcontorted Leftcontorted Ovules per carpel Few 2–5 2 1 Many 1 Many Many 1 Fruit type Capsular Samaralike Non-fleshy, Schizodrupaceous carpous Capsular Drupaceous Capsular Capsular Drupaceous Dispersal mode Anemochorous Anemochorous Sea currents Anemochorous Anemochorous Zoochorous Anemochorous Anemochorous Zoochorous Pollen type Colporate Colporate Colporate Colporate Colporate Porate Pororate/ colporate Colporate Colporate Character Glionnetia 947 Razafimandimbison & al. • A basal grade in the Vanguerieae alliance genus. The genus can be confused with some members of Ixoreae because of its arborescent growth habit, terminal and paniculate inflorescences, and large, 5-merous flowers with left-contorted aestivation. However, Glionnetia bears capsular fruits with many-seeded carpels, while the members of Ixoreae have drupaceous fruits with one-seeded carpels (Table 2). Futhermore, Glionnetia and Crossopteryx have arborescent growth habit, terminal, paniculate inflorescences, left-contorted aestivation, and capsular fruits. However, these genera have very distinct seeds: the former with very small (2–3 mm long), unwinged seeds (Tirvengadum, 1984) and the latter with large, flattened seeds with deeply fimbriate wings (Stoffelen & al., 1996). Additionally, Crossopteryx is restricted to tropical Africa, while Glionnetia is endemic to the Seychelles. For now, we leave Glionnetia unclassified at tribal level pending further analyses. Trailliaedoxa was considered to be a genus with uncertain phylogenetic position within the family Rubiaceae (Smith, 1917; Robbrecht, 1988). Smith (1917) thought that the floral structure of the genus is similar to that of Alberteae sensu Hooker (1873), which is characterized by contorted aestivation and solitary pendulous ovules. On the other hand, its ericoid habit has been compared to that of Spermadictyon of the tribe Paederieae in the subfamily Rubioideae (Smith, 1917). Our Ixoroideae-FourLocus analyses (Fig. 3) place Trailliaedoxa in the Vanguerieae alliance. This position is consistent with the frequent occurrence of contorted aestivation in Ixoroideae, particularly in the Coffeeae and Vanguerieae alliances. Trailliaedoxa is very distinct from other genera in the alliance because of its ericoid habit, densely pubescent styles, and schizocarpous fruits. Based on the results of the analyses presented (see also Table 2), we maintain the generic status of Glionnetia, Jacki­ opsis, and Trailliaedoxa and the tribal status of Jackieae as delimited by Ridsdale (1979). We suggest that Trailliaedoxa and Scyphiphora be recognized at tribal level. However, the formal recognition of the two respective new tribes will be published in another paper focusing on the tribal assessment and relationships in the subfamily Ixoroideae (Kainulainen & al., subm.). A basal grade of monotypic genera in the species-rich Vanguerieae alliance and its conservation implications. — The Ixoroideae-Four-Locus analyses (Fig. 3) reveal a basal grade of morphologically distinct, monotypic genera (Crossop­ teryx, Jackiopsis, Scyphiphora, Trailliaedoxa, and Glionnetia, respectively) in the otherwise species-rich Vanguerieae alliance. In other words, these monotypic genera form a paraphyletic assemblage at the base of the alliance. These results here presented are interesting from both phylogenetic and conservation standpoints, as these genera may be sole representatives of their respective lineages and therefore may carry unique genetic information. Their extinction would mean loss of their entire lineages (see also Rydin & al., 2008; Rana & Ranade, 2009). These monotypic genera could be potential candidates for conservation priorities due to their genetic isolation, however this latter should not automatically be reason for conservation concern. Below we present conservation assessments of these five genera, applying the criteria set in World Conservation Union (IUCN) Red List Categories (IUCN, 2001). 948 TAXON 60 (4) • August 2011: 941–952 ■ Crossopteryx febrifuga is a shrub or small tree species restricted to the savanna woodlands of tropical Africa. The species is common and widespread throughout tropical Africa and is fire resistant due to its thick bark (Gignoux & al., 1997); it is widely used as a medicinal plant across much of tropical Africa but is not considered endangered. At present, it may represent less of a conservation priority, and therefore, we consider it to be Least Concern (IUCN, 2001). ■ Jackiopsis ornata is restricted to Southeast Asia and distributed in the Malay Peninsula, Sumatra, Borneo, and the Riau Archipelago (Ridsdale, 1979). According to Sosef & al. (1998), the species is “reported to be locally frequent but never common in lowland swamp forests and riverine habitats”; the wood of J. ornata is “rarely and only locally used”. It was considered by UNEP-WCMC (2008) to be a timber tree species, which is not in the international trade or of conservation concern. However, the occurrence of this species appears patchy and is not registered in most peat swamp forest inventories (e.g., Kishokumar, 2008). In Southeast Asia, there has been much large-scale conversion of peatlands into commercial agriculture, such as for oil palms (Aiken & Leigh, 1992; Jomo & al., 2004; Kathirithamby-Wells, 2005). In addition, the frequent fires degrading peat swamp forests on Borneo (Sosef & al., 1998) and Sumatra (Anderson & Bowen, 2000) already had a tremendous impact on the natural habitats of J. ornata. Based on the information presented above, we consider J. ornata Endangered (IUCN, 2001). Therefore, an ex situ conservation plan for this unique taxon is strongly encouraged. The gregarious flowering and stately habit of mature J. ornata trees, with spectacular displays of dense, brightly colored inflorescences, should provide an added incentive for future conservation and planting. ■ Scyphiphora hydrophylacea is a mangrove plant. It is distributed from East India throughout the Indo-Malesian region to the West Pacific and northern Australia, Sri Lanka, and Madagascar (Puff & al., 2007). The wide coastal distribution range can be attributed to the trans-oceanic dispersal of its buoyant fruits. Mangrove stands are highly threatened ecosystems and exploited for fuelwood and timber, local constructions, development of salt beds, and conversion to aquaculture ponds (e.g., Tomlinson, 1986; Primavera, 2000). For example, mangrove areas in the Philippines decreased from 428,382 ha (Villaluz, 1953) to 200,500 ha (DENR, 1996) between 1951 and 1995. About 50% of the Philippine mangroves that disappeared from 1950 to 1988 had been converted into culture ponds, making aquaculture the main cause of the mangrove loss in the Philippines (Primavera, 2000). Scyphiphora hydrophylacea is reported to be rare and highly threatened in Sri Lanka and Peninsular India, where seedlings and young plants are scarce. This situation has been attributed to a very low percentage of fertile fruits in S. hydrophylacea and its inability to produce healthy seedlings, which is possibly due to inbreeding depression in small, isolated populations (Hettiarachchi & Jayaratne, 2006). According to Puff & al. (2007), S. hydrophylacea is usually not present in large populations in Thailand. Within its center of distribution in Southeast Asia, there is much alteration and destruction of its habitat. Largely ignored by TAXON 60 (4) • August 2011: 941–952 forestry inventories or conservation projects, the occurrence of S. hydrophylacea is rare or patchy along the more frequently inundated mangrove fringes and adjacent shores. Occurrence is largely insignificant to absent within the main formation that is dominated by taller stands of Rhizophoraceae and other tree species. Moreover, conversion to other land use or subsequent degradation due to adjacent coastal development can drastically reduce mangrove extents (Chan & al., 1993; Sasekumar & Then, 2005). Based on the evidence for concern presented above, we consider S. hydrophylacea Near Threatened (IUCN, 2001) despite its extensive range. ■ Trailliaedoxa gracilis appears to have a narrow distribution range. Endemic to southern China, it grows in rocky habitats and thickets on the mountain slopes of Sichuan and Yunnan at an altitude of 1540 to 3000 m. The conservation status of the genus is currently unknown, as it is only known from a few specimens. Trailliaedoxa gracilis may be a potential candidate for a high conservation priority, however it is poorly known (= Data Deficient; IUCN, 2001). ■ Finally, Glionnetia sericea is confined to a very restricted area less than 1000 ha on Mahé and Silhouette Islands in the Seychelles Archipelago. It is one of two genera of Rubiaceae endemic to the Seychelles and has been considered Vulnerable D2 (IUCN, 2010). However, a recent assessment concluded that the species is Endangered B1ab(iii)+2ab((iii) (Huber, pers. comm.). Glionnetia sericea thrives mainly on high ridges in the mountains (Tirvengadum, 1984); it does not seem to grow well at lower altitudes (K. Beaver, pers. comm.). Conservation in situ is strongly encouraged. ACKNOWLEDGEMENTS The authors thank Anbar Khodanbadeh for help with sequencing; the three anonymous reviewers and Elmar Robbrecht for their constructive criticisms on an earlier version of the paper; the Seychellois authority for approving our research proposal to collect Glionnetia for this study; and the following herbaria for allowing access to their collections: BR, K, L, NY, P, S, SING, TAN, TEF, and UPS. This work is supported by the Swedish Research Council and the Knut and Alice Wallenberg Foundation to BB. 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Strategies for the sustainable use and management of timber species subject to international trade; South East Asia. Cambridge, U.K.: UNEPWCMC. Villaluz, D.K. 1953. Fish farming in the Philippines. Manila: Bookman. Xiao, L.-Q. & Zhu, H. 2007. Paraphyly and phylogenetic relationships in Lasianthus (Rubiaceae) inferred from chloroplast rps16 data. Bot. Stud. 48: 225–232. Appendix. Species sampled for the study. Species, accession nos. [ndhF, rbcL, rps16, trnT­F; an n-dash (–) denotes missing data; superscripts 1–27 refer to literature or GenBank sources, superscripts 28–37 refer to voucher specimens and herbarium acronyms, both given at the end of the Appendix]. Acranthera frutescens Valeton, –, AM1171989, –, –; Acranthera grandiflora Bedd., –, AM1171999, –, –; Alstonia scholaris (L.) R. Br., –, X9176028, –, –; Am­ phidasya ambigua Standl.) Standl., –, Y1184420, –, –; Arcytophyllum aristatum Standl., –, AJ28859529, –, –; Anthocleista Afzel. ex R. Br., –, L1438930, –, –; Anthospermum herbaceum L. f., –, X8362310, –, –; Airosperma vanuense S.P. Darwin, AM9498451,–, FM2047001, FM2071081; Alberta magna E. Mey., AJ2362823 EU8174117 FM2047011 AJ6201184; Aleisanthia rupestris (Ridl.) Ridl., –, –, AF2429026, AF1526605; Aleisanthiopsis distantiflora (Merr.) Tange, HM16435028, EU8174117, EU8174347, EU8174537; Aidia micrantha (K. Schum.) Bullock ex F. White, –, Z6884415, –, –; Argostemma hookeri King, –, Z6878831, –, –; Augusta rivalis (Benth.) J.H. Kirkbr., AM9498461, AM9498421, –, FM2071181; Aulacocalyx jasminiflora Hook.f., –, EU8174137, EF205639 8, EU8174557; Bertiera guianensis Aubl.; AM9498471, AJ2248452, FM2047101, FM2071191 & AF1526705; Boholia nematostylis Merr., AM9498481, FM2047111, AM1173509; Caly­ cophyllum candidissimum (Vahl) DC., AJ2362853, X8362710, FM2047121, AJ84739811; Canthium tetraphyllum (Schweinf. ex Hiern) Robyns, AM9498491, X8364910, FM2047131, FM2071201; Carphalea glaucescens (Hiern) Verdc., –, Z6878931, –, –; Catesbaea spinosa L., –, X8362810, –, –; Chioccoca alba (L.) Hitch., –, L1439430, –, –; Cephalanthus occidentalis L., –, X8362910, –, –; Cinchona pubescens Vahl., AJ23584312, X8363010, FM2047141, AJ34696313; Coc­ cocypselum hirsutum Bartl. ex DC., –, X8714510, –, –; Coffea arabica L., EF04421314, EF04421314, EF04421314, EF04421314; Colletoecema dewevrei (De Wild.) E.M.A. Petit, –, EU14545723, –, –; Condaminea corymbosa (Ruiz & Pav.) DC., –, Y187133, –, –; Coptosapelta diffusa Steenis, –, EU14545223, –, –; Coptosapelta flavescens Korth., –, Y187143, –, –; Coptosapelta montana Korth. ex Valeton, –, EU14545123, –, –; Coussarea macrophylla (Mart.) Müll. Arg., –, Y118473, –, –; Cremaspora triflora ssp. confluens (K. Schum.) Verdc., AM9498501, Z6885615, FM2047151, FM2071211; Cremocarpon lantzii Bremek., –, AM1172229, –, –; Crossopteryx febrifuga (Afzel. ex G. Don) Benth. 1, –, AJ34700913, FM2047161, FM2071221; Crossopteryx febrifuga (Afzel. ex G. Don) Benth. 2, AM9498511, AM1172239, FM2047171, FM2071231; Cruckshancksia hymenodon Hook. & Arn., –, AJ28859929, –, –; Cubanola domingensis (Britton) Aiello, AM1173459, X8363210, FM2047181, FM2071241; Cyclophyllum deplanchei Hook.f., –, EU8174167, EF2056408, EF2056318; Damnacanthus indicus C.F. Gaertn., –, Z6879331, –, –; Danais xanthorrhoea (K. Schum.) Bremek., –, Z6879431, –, –; Declieuxia cordigera Mart. & Zucc. ex Scult. & Schult.f., –, AM1172249, –, –; Dibrachiono­ stylus kaessneri (S. Moore) Bremek., –, AJ61621132, –, –; Diplospora polysperma Valeton, –, AJ28670317, AM1173019, EU14553823; Dunnia sinensis Tutcher 1, –, EU14546723, –, –; Dydimaea alsinoides (Cham. & Schtdl.) Standl., –, Z6879531, –, –; Emmenopterys henryi Oliv., AJ2362943, Y187153, FM2047191, FM2071251; Ernodea littoralis Sw., –, AJ28860129, –, –; Faramea multiflora A. Rich., –, Z6879631, –, –; Ferdinandusa speciosa Pohl., –, AM1172269, –, –; Feretia aerugi­ nescens Stapf, –, Z6885731, –, –; Fernelia buxifolia Lam., –, AJ28670417, –, –; Galium album Mill., –, X8109010, –, –; Gardenia hansemannii K. Schum., AM9498521, AJ31844616, FM2047201, FM2071261; Gelsemium Juss., –, L1439730, –, –; Gentiana L., –, L1439830, –, –; Geophila obvallata Didr., –, AM1172289, –, –; Glionnetia sericea (Baker) Tirveng. 1, HM53621629, HM53622229, HM53622829, HM53623429; Glionnetia sericea (Baker) Tirveng. 2, HM53621730, HM53622330, HM53622930, HM53623530; Glionnetia sericea (Baker) Tirveng. 3, HM53621831, HM53622431, HM53623031, HM53623631; Greenea corymbosa (Jack) Volgt, –, –, AF2429616, AF1526575; Greenea oblonga Craib, HM16437132, –, EU8174597; EU8174397; Greenea sp. 1, –, –, EU8174387, EU8174587; Greenea sp. 2, –, EU8174187, EU8174407, EU8174607; Guettarda uruguensis Cham. & Schltdl., –, X8363810, –, –; Gynochthodes coriacea Blume, –, AJ28860329, –, –; Hedyotis fruticosa L., –, Z6879931, –, –; Hillia triflora (Oerst.) C.M. Taylor; –, X8364210;, –, –,; Houstonia caerutea L., –, AJ28860429, –, –; Hydnophytum formicarum Jack, –, X8364510, –, –; Hypobathrum racemosum (Roxb.) Kurz, AM9498531, AJ28670517, AM1173189, FM2071271; Ixora brachypoda DC., –, EU8174217, EU8174427, EU8174637; Ixora coccinea L., AJ2362993, X8364610, EF2056418, EU8174647; Ixora guineensis Benth., –, EU8174247, EU8174437, EU8174677; Ixora hookeri (Oudem.) Bremek.; –, EU8174257, EU8174437, EU8174677; Ixora margaretae N. Hallé, –, EU8174257, EU8174447, EU8174687; Ixora moorensis (Nadeaud) Fosberg, –, EU8174207, EU8174417, EU8174627; Ixora trilocularis (Balf.f.) Mouly & B. Bremer, –, EU8174177, EU8174377, EU8174577; Jackiopis ornata Ridsdale 1, HM5362133, HM53621933, HM53622533, HM53623133; Jackiopis ornata Ridsdale 2, HM53621434, HM53622034, HM53622634, HM53623234; Jackiopis ornata Ridsdale 3, HM53621535, HM53622135, HM53622735, HM53623335; Keetia gueinzii (Sond.) Bridson, –, –, AM1173239, AJ6201439; Kohautia caespitosa Schnizl., –, Z6880031, –, –; Kraussia floribunda Harv., –, Z6885831, –, –; Lasianthus pedunculatus (Griseb.) Urb., –, Z6880231, –, –; Lerchea bracteata Valeton, AJ28861029; Luculia grandifolia Ghose, –, X8364810, –, –; Luculia gratissima (Wall.) Sweet, AJ01198718, AM1172439, AJ43103619, AJ43091119; Luculia pinceana Hook., –, EU14544723, –, –; Manostachya ternifolia E.S. Martins, AJ61621320; Margaritopsis acutifolia C. Wright, –, AM1172479, –, –; Maschalocorymbus corymbus (Blume) Bremek., –, AJ28861129, –, –; Mitchella repens L., –, Z6880531, –, –; Mitrasacmopsis quadravalvis Jovet, –, AJ61621420, –, –; Morinda citrifolia L., AJ2363003, X8365110, AJ32007816, FM2075891 & AF1526165; Mostuea Didr., –, L144042, –, –; Mussaenda arcuata Poir., AJ2363013, Y1185420, FM2047211, FM2071281; Mussaenda erythrophylla Schumach. & Thonn., –, X836521; Mycetia malayana (G. Don) Craib, –, Z6880631, –, –; Nauclea orientalis (L.) L., –, X8365310, –, –; Nematostylis anthophylla (A. Rich. ex DC.) Baill., FM2076501, FM2076481, FM2047231, FM2071301; Nertera granadensis (Mutis ex L.f.) Druce, –, X8365410, –, –; Neurocalyx zeylanicus Hook., –, Z688073, –, –; Normandia neocaledonica Hook.f., –, AM1172509, –, –; Oldenlandia corymbosa L., –, X8365510, –, –; Ophiorrhiza mungos L., –, X8365610, –, –; Oreopolus glacialis (poepp.) Ricardi, –, AJ28861229, –, –; Paederia foetida L., –, AF33237333, –, –; Palicourea crocea (Sw.) Schult, –, AM1172539, –, –; Parapentas silvatica (K. Schum.) Bremek., –, X8365710, –, –; Pauridiantha paucinervis (Hiern) Bremek., –, Z6881131, –, –; Pavetta abyssinica Fresen., AM9498541, Z6886315, FM2047261, FM2071331; Pentagonia macrophylla Benth., AJ2363033, 951 Razafimandimbison & al. • A basal grade in the Vanguerieae alliance TAXON 60 (4) • August 2011: 941–952 Appendix. Continued. X8365810, FM2047271, FM2071341; Pentas lanceolata (Forssk.) Deflers., –, Z8365931, –, –; Pentodon pentandrus (Schumach. & Thonn.) Vatke. Oesterr., –, X8366010, –, –; Peponidium comorense Arènes, –, –, EF2056468 EF2056328 ; Posoqueria latifolia (Rudge) Roem. & Schult., AM9498551, Z6885015, FM2047281, FM2071351; Pouchetia gilletii De Wild., –, Z6885915 , –, –; Praravinia suberosa (Merr.) Bremek., –, AJ28861629, –, –; Pseudomussaenda flava Verdc., AM9498561, Y1185520, FM2047291, FM2071361; Psychotria kirkii Hiern, –, X8366310, –, –; Psychotria poeppigiana Müll.Arg., –, Z6881831, –, –; Pyrostria hystrix (Bremk.) Bridson, EU14541823, AM1172629, AM1173389, AJ6201684; Razafimandimbisonia minor (Baill.) Kainul. & B. Bremer, AM9498441, EU8174107, EF2056378, EU8174527; Razafimandimbisonia sambiranensis (Homolle ex Cavaco) Kainul. & B. Bremer, –, EU8174317, EF2056458, EU8174747; Retiniphyllum concolor (Spruce ex Benth.) Müll.Arg., –, –, EU82162924 EU82164124; Retiniphyllum maguirei Standl., –, –, EU82163224 EU82164624; Retiniphyllum pilosum (Spruce ex Benth.) Müll.Arg., –, AF33165421, FM2047301, FM2071371; Retiniphyllum schomburgki (Benth.) Müll.Arg., –, –, EU82163124, EU82164524 ; Rhachi­ callis americana (Jacq.) Hitchc., –, X8366410, –, –; Rondeletia odorata Jacq., –, Y1185720, –, –; Rubia tinctorum L., –, X8366610, –, –; Rustia thibaudioides (H. Karst.) Delprete, AJ2363103, Y187163, FM2047311, FM2071381; Sabicea aspera Aubl., –, AY53850822, –, –; Sabicea diversifolia Pers., –, EU14545923, –, –; Sabicea villosa Roem. & Schult., AM9498571, Y1185820, FM2047321, FM2071391; Saldinia sp. 1, –, AM1172699, –, –; Schradera K. Krausse sp., –, Y118593, –, –; Schismatoclada sp., –, AM1172719, –, –; Schizocolea linderi (Hutch. & Dalziel) Bremek., –, AM1172729, –, –; Scyphiphora hydrophylacea C.F. Gaertn. 1, AJ2363113, Y187173, FM2047331, FM2071401; Scyphiphora hydrophylacea C.F. Gaertn. 2, –, –, EU82163424 EU82164824; Sherardia arvensis L., –, X8110610, –, –; Spermacoce laevis Lam., –, Z6882331–, –; Spigelia L., –, Y1186320, –, –; Sipanea biflora (L. f.) Cham. & Schltdl., EU14541323, AY53850922, FM2047341, FM2071411; Sipanea hispida Benth. ex Wernham, EU14541423, EU14545823, EU14549223, AY55510727; Sipanea pratensis Aubl., –, –, AF2430226, AF1526775; Spiradiclis bifida Kutz, –, EU145465, –, –; Steenisia pleurocarpa (Airy Shaw) Bakh.f., –, AM1172799, FM2047351, FM2071421; Strychnos L., –, L1441028, –, –; Tamridaea capsulifera (Balf.f.) Thulin & B. Bremer, –, Y1186020, FM2047361, FM2071431; Tennantia sennii (Chiov.) Verdc. & Bridson, AM9498581, AM9498431, FM2047371, FM2071441; Thecorchus wauensis (Schweinf. ex Hiern) Bremek., –, AM1172829, –, –; Theligonum cynocrambe L., –, X8366810, –, –; Tocoyena pittieri (Standl.) Standl., AM9498591, –, FM2047381, FM2071451; Trailliaedoxa gracilis W.W. Sm. & Forrest 1, HM16439936, HM16418236, HM16422636, HM16433836; Trailliaedoxa gracilis W.W. Sm. & Forrest 2, HM16440037, HM16418337, HM16422737, HM16433937; Tricalysia cryptocalyx Baker, –, Z6885415, AF00408826, AM99948725; Trichostachys aurea Hiern, –, EU14546223, –, –; Trichostachys sp., –, AJ28862629, –, –; Urophyllum ellipticum (Wight) Thwaites, –, X8367010, –, –; Vangueria madagascariensis J.F. Gmel., AJ1308403, X8367010, EU82163624, FM2071461; Virectaria major (K. Schum.) Verdc., –, Y1186120, –, –; Xanthophytum borneense (Valeton) Axelius, –, EU14546623, –, –; Wendlandia tinctoria (Roxb.) DC., AM9498601, FM2076491, FM2047391, FM2071471. Published sequences: 1, Kainulainen & al. (2009); 2, Andreasen & al. (1999); 3, Bremer & al. (1999); 4, Lantz & Bremer (2004); 5, Rova & al. (2002); 6, Rova, J.H.E. (Genbank unpub.); 7, Mouly & al. (2009); 8, Mouly & al. (2007); 9, Bremer & Eriksson (2009); 10, Bremer & al. (1995); 11, Alejandro & al. (2005); 12, Backlund & al. (2000); 13, Razafimandimbison & Bremer (2002); 14, Samson & al. (2007); 15, Andreasen & Bremer (1996); 16, Novotny & al. (2002); 17, Andreasen & Bremer (2000); 18, Oxelman & al. (1999); 19, Bremer & al. (2002); 20, Bremer & Thulin (1998); 21, Andersson, L. (Genbank unpub.); 22, Andersson & Antonelli (2005); 23, Rydin & al. (2008); 24, Cortes-B. & al. (2009); 25, Tosh & al. (2009); 26, Andersson & Rova (1999); 27, Delprete & Cortes-B. (2004) . Voucher information: 28, Tange 46977 (AAU); 29, Beaver 3 (S); 30, Beaver 17 (S); 31, Beaver 27 (S); 32, Larsen K. & Larsen S.S. 33451 (P); 33, Tan s.n. (Malaysia), cA047; 34, Tan s.n. (Malaysia), cA048; 35, Tan s.n. (Malaysia), cA049; 36, Yü 1348 (HUH); 37, Boufford & al. 35041 (MO) . 952