Bioorganic & Medicinal Chemistry 17 (2009) 5688–5695
Contents lists available at ScienceDirect
Bioorganic & Medicinal Chemistry
journal homepage: www.elsevier.com/locate/bmc
Antimicrobial and immunomodulatory properties of prenylated xanthones
from twigs of Garcinia staudtii
Joseph Ngoupayo a,*, Turibio Kuiate Tabopda b, Muhammad Shaiq Ali c
a
Département de Pharmacie et Pharmacothérapeutique Africaine, Faculté de Médecine et des Sciences Biomédicales, Université de Yaoundé 1, BP 1364 Yaoundé, Cameroun
Département de Chimie Organique, Université Yaoundé I, BP 812 Yaoundé, Cameroun
c
H.E.J. Research Institute of Chemistry, University of Karachi, Karachi-75270, Pakistan
b
a r t i c l e
i n f o
Article history:
Received 8 March 2009
Revised 30 May 2009
Accepted 5 June 2009
Available online 13 June 2009
Keywords:
Clusiaceae
Garcinia staudtii
Xanthones
Antibacterial activity
Immunomodulatory activity
a b s t r a c t
Phytochemical investigation of the methanol extract of the twigs of Garcinia staudtii yielded four new
prenylated xanthones, staudtiixanthones A D (1–4), along with eleven known compounds. Their structures were determined by analysis of 1D and 2D NMR spectra and by comparison of spectroscopic data
with those previously reported. Some of these compounds have been evaluated for antibacterial activity
against methicillin-resistant Staphylococcus aureus (MRSA). The new compounds were also screened for
phagocyte chemiluminescence, neutrophil chemotaxis, T-cell proliferation, cytokine production from
mononuclear cells and cytotoxicity. They were found to exhibit potent immunomodulatory activities.
Ó 2009 Elsevier Ltd. All rights reserved.
1. Introduction
Immunomodulation is a very broad term which denotes any
changes in the immune response and may involve induction,
expression, amplification or inhibition of any part or phase of the
immune response. Modulation may be very specific limited to a
given antigen/agent or non-specific, with a general effect on immune response. Stimulation of the immune response is desired
for certain people such as immunocompromised patient, whereas
suppression of the immune response is thought for others, such
as transplant recipient, allergic and inflammatory diseased
patients.1 Natural compounds with potential immunostimulating
activity studied can be classified as high and low molecular weight
compounds. These compounds belong to different classes of substances. Terpenoids, phenolic compounds, and alkaloids dominate
among low molecular weight compounds, and polysaccharides
dominate among the high molecular weight compounds.
Garcinia staudtii is a morphologically distinctive species of flowering plant in the Clusiaceae family. It is confined to forested areas
extending from south-east Nigeria to Cameroon.2 Medicinal plants
of the genus Garcinia are known to be rich in prenylated xanthones.3 Xanthone constituents have been reported to possess several
biological activities, such as antibacterial, antimalarial, radical
scavenging, cytotoxicity, inhibition of cyclooxygenase, and prosta-
* Corresponding author. Tel.: +92 237 96853797; fax: +92 237 22221873.
E-mail address: jngoupayo@yahoo.fr (J. Ngoupayo).
0968-0896/$ - see front matter Ó 2009 Elsevier Ltd. All rights reserved.
doi:10.1016/j.bmc.2009.06.009
glandin E2 activities.4–8 In our continuing phytochemical investigation of Garcinia plants found in Cameroon, we have examined
the twigs of G. staudtii. To our knowledge, no chemical investigation of G. staudtii has been reported. This investigation led to the
isolation and structural elucidation of four new prenylated
xanthones (1–4) and eleven known compounds; friedelin, lupeol,
a- and b-amyrin, b-sitosterol, stigmasterol, a-mangostin (5),9
garcinone B (7),9 demethylcalabaxanthone (6),10 Gartanin (8),11
and xanthone V1 (9).12 The isolated xanthones were tested for their
antimicrobial activities against methicillin-resistant Staphylococcus
aureus (MRSA). New xanthones 1–4 were also evaluated for their
immunomodulatory properties.
2. Results and discussion
2.1. Chemistry
The CHCl3 fraction of the twigs of G. staudtii were subjected to
repeated column chromatography to yield four new prenylated
xanthones (1–4) together with friedelin, lupeol, a- and b-amyrin,
b-sitosterol, stigmasterol and five known xanthones (5–9) (Fig. 1).
Staudtiixanthone A (1), obtained as a colorless amorphous powder, showed a [M]+ peak at m/z 396.1563 in the HREIMS, corresponding to a molecular formula of C23H24O6. The IR spectrum
showed the presence of hydroxyl groups (3401–3448 cm 1), a chelated carbonyl group (1647 cm 1), and benzene rings (1587, 1558,
and 1513 cm 1). The UV (MeOH) spectrum exhibited characteristic
J. Ngoupayo et al. / Bioorg. Med. Chem. 17 (2009) 5688–5695
5689
O
R6
O
OH
5'
OH
HO
8
R5
7
9a
O
4a
8a
5
1
R1
A
B
4b
R4
9
3
R3
O
OH
3
O
8''
3''
8''
7''
3''
2''
7''
2''
4''
R2
1 R1 = R2 = prenyl; R3 = R5 = OH; R4 = R6 = H
5 R1 = R6 = prenyl; R2 = R3 = H; R4 = OH; R5 = OCH3
8 R1 = R2 = prenyl; R3 = R6 = OH; R4 = R5 = H
R5
O
OH
4
4'
2'
4''
O
OH
O
H 3C
OH
O
O
O
4'
R4
3'
4
8'
R3
O
O
8'
3'
7'
2'
7'
R2
2'
4'
O
R1
OH
O
2 R1 = prenyl; R2 = R4 = OH; R3 = R5 = H
6 R1 = R2 = R3 = H; R4 = OH; R5 = prenyl
9 R1 = R4 = R5 = H; R2 = R3 = OH
HO
O
OH
7
Figure 1. Chemical structures of isolated xanthones 1–9 from Garcinia staudtii.
absorption bands of the xanthone13 at kmax 246, 276, 308, and
338 nm. 1H and 13C NMR data (Table 1), aided by DEPT and HMQC
experiments, disclosed the presence of a carbonyl, twelve sp2 quaternary carbons (six of which were oxygen bearing), four sp2
methines, two sp3 methylenes, and four methyl groups. The initial
analysis of the NMR spectral data of 1 indicated the presence of a
Table 1
1
H and 13C NMR data of 1, 2, 3, and 4 at 400 MHz (for 1H NMR) and 100 MHz (for
xanthone skeleton and two prenyl moieties. The 1H NMR spectrum
(Table 1) showed a pair of two meta-aromatic proton signals at dY
6.15 (1H, d, J = 1.9 Hz) and 6.47 (1H, d, J = 1.9 Hz) as well as a
chelated hydroxyl signal at dY 13.34 (1H, s). In addition, typical
signals of two prenyl groups [four allylic methyl groups at dY
1.69 (3H, s, H3-500 ), 1.70 (3H, s, H3-50 ), 1.73 (3H, s, H3-40 ), and
13
C NMR) in CDCl3, d in ppm, J in hertz
J. Ngoupayo et al. / Bioorg. Med. Chem. 17 (2009) 5688–5695
5690
O
OH
O
HO
HO
O
O
OH
H3CO
OH
O
O
4
1
COSY
HMBC
Figure 2. Selected COSY and HMBC correlations for compounds 1 and 4.
1.75 (3H, s, H3-400 ); two pairs of methylene protons at dY 3.25 (2H,
br d, J = 6.8 Hz, H2-100 ) and 3.32 (2H, br d, J = 7.1 Hz, H2-10 ); and two
olefinic protons at dY 5.09 (1H, t, J = 7.1 Hz, H-20 ) and 5.13 (1H, t,
J = 6.8 Hz, H-200 )] were observed in the 1H NMR spectrum of 1.
The six substituents on the xanthone skeleton were determined
on the basis of the HMBC spectral analysis (Fig. 2). The locations
of the two prenyl moieties were placed at the C-2 (dC 107.9) and
C-4 (dC 106.2) positions by the HMBC correlations of H2-10 /C-1
(dC 160.4), H2-10 /C-2, and H2-10 /C-3 (dC 156.1), and H2-100 /C-3, H2100 /C-4 and H2-100 /C-4a (dC 162.9) in the HMBC spectrum of 1
(Fig. 2), respectively. The substitution pattern of ring B was elucidated by the interrelated peaks of H-6/C-4b (dC 136.4), H-6/C-5
(dC 146.4), H-6/C-8 (dC 106.3), H-8/C-6 (dC 102.0), H-8/C-4b, and
H-6/C-9 (dC 179.5) in the HMBC spectrum. Therefore, three hydroxyl groups were positioned to the C-5, C-7 (dC 156.7), and C-1 positions, and the remaining two oxygen-bearing quaternary carbons
were assigned to the C-4a and C-4b positions, respectively. These
results established the structure of staudtiixanthone A (1) as
1,3,5,7-tetrahydroxy-2,4-diprenylxanthone.
Staudtiixanthone B (2), which is obtained as a colorless amorphous powder, has a molecular formula C23H22O6 based on the
results of HREIMS (m/z 394.1411). The IR spectrum showed
absorption bands for hydroxyl and conjugated ketone functional
groups. The UV spectrum of 2 exhibited bands very similar to those
of 1. The 1H and 13C NMR spectra (Table 1) aided by DEPT and
HMQC experiments disclosed the presence of 23 carbons that were
arranged similarly to those of 1, except for the replacement of one
prenyl group with a 2,2-dimethylpyran ring [dH 1.47 (6H, s), 5.78
and 7.03 (1H, each, d, J = 10.2 Hz)]. The presence of the 2,2-dimethylpyran moiety was further confirmed both by the 13C NMR
spectrum which showed characteristic signals at dC 28.0 (C-50 , C60 ), 78.8 (C-20 ), 116.6 (C-40 ), and 127.6 (C-30 )14 and by the EIMS
on which the base peak at m/z 379 [M 15]+ was observed. As in
(1), the 1H NMR spectrum of (2) (Table 1) showed the characteristic
signals of two meta-aromatic protons [dH 6.15 (1H, d, J = 2.1 Hz)
and dH 6.51 (1H, d, J = 2.1 Hz)] located on the ring B. There
remained the placement of the dimethylpyran ring and the prenyl
group. In the HMBC spectrum, the proton resonating at dH 7.03 (H40 ) was correlated with oxygenated aromatic carbons at dC 156.7
(C-1) and 158.1 (C-3) through 3J, which implied that the dimethylpyran ring oxygen belongs to the hydroxyl group at C-3.
Hence, from the above analysis, the 2,2-dimethylpyran and prenyl
groups were concluded to be located at C-2/C-3 and C-4, respectively. The chemical shifts of C-1 to C-4a were in agreement with
those reported for inoxanthone.15 The structure of staudtiixanthone B (2) was, therefore, concluded to be 1,5,7-trihydroxy-4-prenyl-20 ,20 -dimethylpyrano[50 ,60 :2,3]xanthone.
Staudtiixanthone C (3) was isolated as a colorless amorphous
powders and gave the same molecular formula C23H22O6 as (2)
by HREIMS ([M]+ at m/z 394.1409). Both compounds displayed
similar NMR features (Table 1), UV absorption bands, and the same
fragmentation patterns in the EIMS, suggesting that 2 and 3 were
isomers. The 1H NMR, 13C NMR, HSQC, HMBC, and 1H–1H COSY
spectra revealed the presence of one dimethylpyran ring, one prenyl group, and one hydrogen-bonded hydroxy group. Comparison
of the 13C NMR spectral data with those of 2 revealed a significative
difference mainly for the carbon shifts at ring A. The HMBC correlations observed for 3 were in perfect agreement with an angular
arrangement of the pyrano unit. In this respect, the HMBC spectrum showed correlations between the proton resonating at dH
3.32 (2H, br d, J = 7.8 Hz, H-10 ) and aromatic carbons at dC 160.9
(C-1) through 3J, which suggested the placement of the prenyl
group at C-2. Strong NOE interaction observed between the prenyl
protons at dH 3.32 (H2-10 ) and chelated proton at dH 13.47 (1-OH) in
the NOESY spectrum further supported the placement of the prenyl
group at C-2 and the 2,2-dimethylpyran ring at C-3/C-4, with the
furan ring oxygen at C-3. On the basis of the above data, the structure of staudtiixanthone C (3) was assigned as 1,5,7-trihydroxy-2prenyl-20 ,20 -dimethylpyrano[50 ,60 :3,4]xanthone.
Staudtiixanthone D (4) was obtained as yellow amorphous
powder. It was formulated as C24H26O6 on the basis of its HREIMS ([M]+ (m/z 410.1727). Its IR spectrum exhibited vibration
bands due to hydroxyl (3454 cm 1) and conjugated carbonyl
(1647 cm 1) groups. The UV absorptions (237, 260, 321, and
360 nm) indicated (4) to be a xanthone derivative. The 1H
NMR of (4) revealed the presence of one chelated hydroxyl
group at dH 12.78 (1H, s, 1-OH), one methoxyl group at dH 3.61
(3H, s), two isolated aromatic protons at dH 6.24 (1H, s) and
6.83 (1H, s), and two 2,2-dimethylchroman rings [dH 3.47, 2.79
(2H each, t, J = 6.8 Hz), 1.88, 1.73 (2H each, t, J = 6.8 Hz), 1.39,
1.31 (6H each, s)]. The chelated hydroxyl proton (1-OH) gave a
3
J peak with a methine aromatic carbon at dC 100.1 (C-2) which
was correlated to the aromatic proton (dH 6.24, H-2) in the
HMQC spectrum. A dimethylchroman ring was then located at
C-3/C-4. This was confirmed by HMBC correlations of H-2 (dH
6.24)/C-9a, H-2/C-4 (dC 101.0), H-40 (dH 2.79)/C-3 (dC 161.6),
and H-40 /C-4a (dC 159.7). The location of the singlet aromatic proton (dY 6.83), the methoxyl group (dY 3.61), and the dimethylchroman unit in the B ring was established by HMBC correlations.
Particularly noticeable feature was the highly deshielded position
of the methylene protons of a 2,2-dimethylpyran unit [dH 1.88(H300 ) and 3.47 (H-400 )]. Such structural feature suggests this functionality to be peri to the carbonyl group. The methoxyl group was assigned to C-6 based on the HMBC correlations of H-5/C-4b (dC
139.6), H-5/C-8a (dC 110.7), H-5/C-7 (dC 140.1), and CH3O/C-6 (dC
150.9). All assignments were compatible with the location of the
2,2-dimethylchroman units in 4. On the basis of the above
evidences, the structure of staudtiixanthone D (4) was elucidated as 1-hydroxy-6-methoxy-20 ,20 -dimethyldihydropyrano[50 ,60 :
3,4]-200 ,200 -dimethyldihydropyrano [500 ,600 :7,8]xanthone.
J. Ngoupayo et al. / Bioorg. Med. Chem. 17 (2009) 5688–5695
Table 2
Compound
1
2
3
4
5
6
7
8
9
MIC (lg/mL)
16
32
128
64
32
128
128
32
—
2.2. Biological activity
2.2.1. Antibacterial activity of isolated compounds
All xanthones were tested for their antibacterial activities
against MRSA. Staudtiixanthone A (1), B (2), a-mangostin (5), and
Gartanin (8) exhibited a moderate activity with a MIC of 16 and
32 lg/mL while staudtiixanthones C (3), D (4), demethylcalabaxanthone (6), and garcinone B (7) showed less activity with an equal
MIC value of P64 lg/mL. From these results, xanthones such as 1,
2, 5, and 8 with the C-4 free prenyl group had better activity than 3
and 4 with modified prenyl group at C-4. In addition, compounds
1–3 with the 5,7-dihydroxy groups showed a better activity than
the others (Table 2).
2.2.2. Effect on phagocytes oxidative burst
Phagocytic cells on activation induce the release of reactive oxygen free radicals (oxidative burst), which are then quantified by a
luminol-enhanced chemiluminescence assay. We investigated the
inhibitory/stimulatory effects of compounds 1–4 on the immune
system in the elimination of foreign materials. Only compound 4
showed stimulatory activity with mononuclear cells (24%), at the
5691
lower concentration (3.1 lg/mL) tested (Fig. 3a). Meanwhile at
the higher concentrations of 25 and 50 lg/mL, compound 4 inhibited mononuclear cells ROS activity (59% and 70%, respectively). All
tested compounds were found to have potential in suppressing
phagocytosis activity of neutrophils and mononuclear cells in a
dose-dependent manner (Fig. 3). However, they were less active
in suppressing the activity of the monocytes compared to that of
neutrophils. Compound 1, the most active, strongly suppressed
the neutrophils and mononuclear cells with activities up to 73%
at 3.1 lg/mL (Fig. 3). The inhibition of the oxidative burst was
not due to cytotoxic effects as none of these compounds were
found to exhibit cytotoxicity.
2.2.3. Effect on T-cell proliferation
The anti-proliferation effect of compounds 1–4 was determined
by measuring the inhibition of phytohemagglutinin (PHA)-induced
T-cell proliferation by determining radioactive thymidine incorporation. All test compounds exhibited a strong suppressive effect on
the PHA-stimulated T-cell proliferation in dose-dependent manner
with IC50 of 6.2, 6.4, 12.7, and 3.7 lg/mL, respectively (Fig. 4). A very
potent and dose-dependant activity was observed with compounds
1, 2, and 3. Both compounds had significantly suppressed PHA T-cell
proliferation activity of PBMN cells. The earlier compounds had the
most potent (94.3% and 97.1%, respectively) suppressive action at
12.5 lg/mL. Compound 4 had the most potent suppressive effect
on T-cell response at lower concentrations. It was found significantly
active in suppressing the stimulatory action of the PHA at 3.1 lg/mL
(44.9%) and 6.125 lg/mL (70.4%).
Figure 3. Chemiluminescence effect of compounds 1–4 on oxidative burst using mononuclear cells (a) and neutrophils (b). Each plot and error bar represent readings ± SD of
three repeats.
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J. Ngoupayo et al. / Bioorg. Med. Chem. 17 (2009) 5688–5695
Figure 4. Effect of compounds on phytohemagglutinin (PHA) T-cell proliferation. The bar graph represents effect of various concentrations of the test compounds 1–4 after
72 h incubation with peripheral blood mononuclear cells at 37 °C. Effect of compounds on T-cell proliferation response is compared with non-proliferated (A) and proliferated
(B) cells. Each bar represents the mean value of triplicate reading ± SD.
2.2.4. Effect on MDBK cell proliferation
When compounds 1–4 were evaluated for their cytotoxicity on
Mavin Darby Bovine Kidney (MDBK) cells, no toxic effect was observed after 72 h of incubation (Fig. 5) at concentrations as high
as 50 lg/mL. Therefore, various doses up to 50 lg/mL were used
during present investigation.
2.2.5. Effect on cytokine release by peripheral blood
mononuclear cells (PBMCs)
The suppressive effect of the compounds 1–4 was tested on the
release of selected cytokine including IL-2, IFN-c, and IL-4 by PHAinduced lymphocytes. Results shown in Figure 6 indicate that compounds 1–3 strongly inhibited IL-2, IL-4, and INF-c release in a
dose-dependent manner from PHA-activated lymphocytes. Compound 4 inhibited IL-2 and IL-4, but have less effect on the inhibition of INF-c. Compound 1 with two unmodified prenyl groups was
found to exert a very potent suppressive effect on IL-2, IFN-c, and
IL-4 production. The lowest concentration (0.5 lg) was found to be
very potent in inhibiting IL-2 (68.2%) and IFN-c (62.5%). IL-4 production was suppressed in the presence of compound 1 up to
1 lg/mL (88.1%). However, the most potent IL-4 (40.6%) suppression at low concentration (0.5 lg) was noted with compound 4.
2.2.6. Effect on polymorphoneutrophil cells chemotaxis
Effect of the test compounds on PMN chemotaxis in response to
a chemotactic peptide formyl-methionylleucyl-phenylalanine
(fMLP 10 8) was assayed using the Boyden chambers. PMNs were
incubated with three different concentrations (2.5, 5, and 25 lg/
mL) of each compound for 1 h and the difference in the cell movement was studied. Prednisolone was run as a positive control. The
data presented in Figure 7 show that all tested compounds exhibited potent suppressive activity on fMLP-induced PMNs movement
and caused about 27–46% inhibition of chemotaxis at test concentrations, which did not change significantly with concentrations.
Compound 3 had the most potent suppressive activity on chemotaxis. It exerted a very potent suppressive effect (46%) at 25 lg/
mL. However with 1 h pre-incubation, potent suppression was observed at lower concentrations too (42.2% at 5 lg/mL and 36.4 at
2.5 lg/mL). A similar effect was observed with the other
compounds.
3. Conclusions
A number of assays, which include phagocyte chemiluminescence, neutrophils chemotaxis, T-cell proliferation assay, cytokine
production from mononuclear cells, and cytotoxicity evaluation
were used to investigate the immunomodulating effect of new
prenylated xanthones on different immune cells. The results
obtained show that compounds exert inhibitory effect on phagocytic activity of PMNs and PBMCs. It was also found to interfere
with the phytohemagglutinin (PHA) T-cell activation in a dosedependant manner. The impaired incorporation of tritiated thymi-
Figure 5. Effect of various concentrations of compounds 1–4 on the viability of MDBK cells after 72 h incubation. All values are the mean of three replicated.
J. Ngoupayo et al. / Bioorg. Med. Chem. 17 (2009) 5688–5695
5693
Figure 6. Effect of compounds 1–4 on cytokine production. Each bar represents the mean value of reading ± SD. (a) IL-2 level = 528.5 ± 34.52, (b) INF-c level = 4033.7 ± 180,
and (c) IL-4 level = 120.5 ± 18.1.
Figure 7. Activity comparison of compounds 1–4 with Prednisolone on Cell Movement. Buffer = no fMLP, Control = fMLP + DMSO fMLP (Formyl-Leucyl-Phenylalanine).
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J. Ngoupayo et al. / Bioorg. Med. Chem. 17 (2009) 5688–5695
dine into cellular DNA may not be due to toxic effect since
compounds were found to be nontoxic against MDBK cell line with
a similar incubation time. The effect of compounds on T-cell
proliferation was further confirmed by suppression of IL-2, IL-4,
and IFN-c production. The data presented above indicated that C5 substitution on xanthones nucleus influences the immunosuppressive activity. The 5-OH substitution contributes in activity,
while unsubstitution at this position decreases the activity.
Although 1 is the most active compound of the series, the only difference between compounds is the dose concentration. A possible
structural feature, which influences the activity, is the substitution
pattern. Compound 1 with two free prenyl groups has better activity. The activity differences between 2 and 3 are clearly due to the
presence or absence of functional groups (prenyl or dimethylpyran
ring) at positions 1, 2, and 3. Compound 4 with two modified prenyl groups is the less active compound in the series in most assays.
However, further mechanism-based studies are required for a better understanding of the mechanism of action of xanthones on
immune response. From the results obtained, we can conclude that
the testing compounds are clearly exerting immunomodulatory
activity on the system used and could be suitable immunomodulatory lead compounds for future research.
4. Experimental
4.1. General
IR spectra were recorded on a JASCO 302-A spectrophotometer
in CHCl3. The EI-MS (ionization voltage 70 eV) was measured on a
Varian AAT 311A mass spectrometer, and HR-EI-MS was taken on a
JEOL HX110 mass spectrometer. 1D and 2D NMR spectra were run
on Bruker AMX 400 MHz NMR spectrometer. Proton-detected heteronuclear correlations were measured using HSQC (optimized for
1
JCH = 145 Hz) and HMBC (optimized for JCH = 8 Hz). The chemical
shifts are given in ppm (d), relative to TMS as an internal standard,
and coupling constants are expressed in hertz. Column chromatography was carried out on silica gel (70–230 mesh, Merck) and flash
chromatography was carried out on silica gel (230–400 mesh,
Merck). TLC was performed on Merck precoated aluminum Silica
Gel 60 F254 sheets, and compounds were detected using ceric sulfate spray reagent. A molecular device spectrophotometer was
used for measurement of enzyme inhibition.
4.2. Plant material
The twigs of G. staudtii were collected in Yaounde (Mount Elunden), Center Province of Cameroon by Mr. Nana Victor (National
Herbarium of Yaounde, Cameroon) in June 2008 and a voucher
specimen No. HNC-167341 was deposited.
4.3. Extraction and isolation
The air-dried and ground twigs (1270 g) of G. staudtii were
extracted successively with n-C6H14, CHCl3, and MeOH, respectively, using a Soxhlet apparatus. After solvent removal in vacuo,
the CHCl3 extracts (12 g) were subjected to silica gel column, chromatography with a gradient of n-C6H14–EtOAc (5% increment of
polar solvent for 500 mL of each proportion) to afford six fractions
from TLC profile. Fraction 2 (3015 mg) was subjected to a silica gel
column eluting with n-C6H14–EtOAc (19:1–4:1) to afford friedelin
(480 mg), b-sitosterol (920 mg), stigmasterol (186 mg), lupeol
(31 mg), and mixtures of a- and b-amyrin (215 mg). Fraction 3
(918 mg) was subjected to a silica gel column eluting with nC6H14–EtOAc (3:2) to afford six major fractions, F3a–F3f. Fractions
3a (48 mg), 3b (54 mg), 3c (193 mg), and 3d (109 mg) were indi-
vidually recrystallized with hexane to produce garcinone B (7)
(7 mg), xanthone V1 (9) (13 mg), staudtiixanthone C (3) (75 mg),
and staudtiixanthone D (4) (58 mg), respectively. Fractions F3e
and F3f (285 mg) were further fractionated on a silica gel column
(n-C6H14–EtOAc, 1:1) and then on a Sephadex LH-20 column (in
MeOH) to afford demethylcalabaxanthone (6) (18 mg) and
a-mangostin (5) (13 mg). Similarly, purification of fraction 4
(1150 g) using column chromatography (silica gel, n-C6H14–EtOAc
2:3 to 3:7 in 5% increments of the EtOAc) gave staudtiixanthone A
(1) (81 mg), staudtiixanthone B (2) (41 mg), and Gartanin (8)
(9 mg) from two successive column chromatographies.
4.3.1. Staudtiixanthone A (1)
Colorless amorphous powder, mp. 201 °C. UV (MeOH) kmax nm
(log e): 246 (4.57), 276 (3.93), 308 (3.69), 338 (3.81). IR mmax
(KBr) cm 1: 3401, 3418, 3448, 1647, 1587, 1558, 1513, 1107. EIMS
(probe) 70 eV, m/z (rel. int.): 396 [M]+ (18), 381 (17), 353 (91), 341
(29), 325 (31), 327 [M C5H9]+ (71), 299 (100), 258 [M 2C5H9]+ (7),
230 (21), 69 (45). HREIMS m/z 396.1563 (calcd for C23H24O6:
396.1573). 1H and 13C NMR data: see Table 1.
4.3.2. Staudtiixanthone B (2)
Colorless amorphous powder, mp. 214 °C. UV (MeOH) kmax nm
(log e): 277 (3.95), 290 (3.94), 308 (3.70), 341 (3.81). IR mmax
(KBr) cm 1: 3451, 2928, 1621, 1562, 1503, 1126, 761. EIMS (probe)
70 eV, m/z (rel. int.): 394 [M]+ (38), 379 [M CH3]+ (61), 310
[M CH3–C5H9]+ (63), 297 (100), 281 (93), (69 (43). HREIMS m/z
394.1411 (calcd for C23H22O6: 394.1416). 1H- and 13C NMR data:
see Table 1.
4.3.3. Staudtiixanthone C (3)
Colorless amorphous powder, mp. 223 °C. UV (MeOH) kmax nm
(log e): 289 (4.01), 307 (3.78), 341 (3.80). IR mmax (KBr) cm 1:
3440, 1621, 1560, 1499. EIMS (probe) 70 eV, m/z (rel. int.): 394
[M]+ (38), 379 [M CH3]+ (61), 310 [M CH3–C5H9]+ (63), 297
(100), 281 (93), (69 (43). HREIMS m/z 394.1409 (calcd for
C23H22O6: 394.1416). 1H and 13C NMR data: see Table 1.
4.3.4. Staudtiixanthone D (4)
Yellow amorphous powder. mp 208–209 °C. UV kmax (MeOH)
nm (log e): 237 (4.31), 260 (4.45), 321 (4.23), 360 (3.87). IR mmax
(KBr) cm 1: 3454, 2943, 1647, 1608, 1511, 1149. EIMS (probe)
70 eV, m/z (rel. int.): 410 [M]+ (26), 395 (12), 379 (63), 351 (81),
367 (100), 351 (11), 333 (21). HREIMS m/z 410.1727 (calcd for
C24H26O6: 410.1729). 1H and 13C NMR data: see Table 1.
4.4. Biological assays
4.4.1. Antibacterial activity
In vitro antibacterial activity was screened against methicillinresistant S. aureus (MRSA) using agar-well diffusion method.16 2–
8 h old bacterial inoculums containing approximately 104–106 colony forming units (CFU)/mL were used in these assays. The wells
were dug in the media with the help of a sterile metallic borer with
centers at least 24 mm. Recommended concentration (100 lL) of
the test sample (1 mg/mL in DMSO) was introduced in the respective wells. The plates were incubated immediately at 37 °C for 24 h.
Activity was determined by measuring the diameter of zones
showing complete inhibition (mm). In order to clarify any participating role of DMSO in the biological screening, separate studies
were carried out with the solutions alone of DMSO and they
showed no activity against the bacterial strain.
4.4.2. Chemiluminescence assay
Luminol-enhanced chemiluminescence assay was performed, as
described by Helfand et al.17 Briefly, neutrophils (1 107) or
J. Ngoupayo et al. / Bioorg. Med. Chem. 17 (2009) 5688–5695
monocyte (1 106), suspended in Hank0 s balance salt solution
with calcium and magnesium (HBSS++), were incubated with
50 lL of compounds concentrations 3.1–50 lg/mL for 30 min. To
each well, 50 ll (20 mg/mL) zymosan (Sigma Chemical Co., USA),
followed by 50 ll (7 10 5 M) luminol (G-9382 Sigma Chemical
Co.) and then HBSS++ was added to adjust the final volume to
0.2 mL. HBSS++ alone was used as a control. Chemiluminescence
peaks were recorded with the Luminometer (Luminoskan RS
Labsystem, Finland). The luminometer was set with repeated scan
mode, 50 scans with 30 s intervals and one second point measuring
time. Various concentrations of compounds 1–4 were incubated
with isolated polymorphoneutrophils (PMNs) and mononuclear
cells (MNCs) for 30 min. The compounds activity was compared
with that of the untreated samples (control) in the chemiluminescence assay.
4.4.3. T-Cell proliferation assay
Peripheral blood mononuclear cells (PBMCs) were isolated from
heparinized venous blood of healthy adult donor by Ficoll–Hypaque gradient centrifugation.18 Cell proliferation was evaluated
by a standard thymidine incorporation assay following a method
reported by Nielsen et al.19 Briefly, cells were cultured at a concentration of 5 105 cells/mL in a 96-well round-bottomed tissue culture plate (Nalge Nunc. Inter.). Cells were stimulated with 5 lg/mL
of PHA (Sigma Chemical Co., USA). Various concentrations of compounds were added to obtain final concentrations of 3.1–100 lg/
mL, each in triplicate. Plates were incubated for 72 h at 37 °C in
5% CO2 incubator. Cultures were pulsed later with 0.5 lCi/well tritiated thymidine (Amersham Pharmacia Biotech, Sweden), and further incubated for 18 h. Cells were harvested and the tritiated
thymidine incorporation was measured by a liquid scintillation
counter (LS 6500, Beckman Coulter, USA). Results were expressed
as mean count per minute (CPM).
4.4.4. Cytotoxicity evaluation
The experiment was performed according to the method
reported earlier20 with some modification. Briefly MDBK adherent
cells (2 105 cells/mL) were incubated with serial concentrations
of compounds (0.195–50 lg/mL) for three/five days. The supernatant was removed and 50 ll of MTT solution was then added. After
2–3 h of incubation at 37 °C, the culture medium was carefully
removed, and 50 ll of 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) solution (2 mg/mL) was added to each
well. Plates were incubated for an additional 2–3 h at 37 °C in a tissue culture incubator. After the MTT solution was aspirated off and
cells were washed with phosphate buffer saline (PBS), 200 ll of
DMSO was added to dissolve the blue insoluble MTT formazan produced by mitochondrial dehydrogenase. The plate was agitated at
37 °C for 15 min and then read at 540 nm using microplate readers
(SpectraMax PLUS384, Molecular Devices, USA). The percentage of
viable cells was calculated as the relative ratio of optical densities
(ODs).
4.4.5. Neutrophil chemotaxis
Chemotaxis was measured using a modified Boyden chamber
(Neuro Probe, Cabin John, MD, USA), as previously described.21
Briefly to the lower compartment 25 lL of the formyl-methionyl-leucyl-phenylalanine (fMLP) (10 8 M) chemoattractant (Sigma
Chemical Co., USA) was added. The upper compartment was separated from the lower compartment by a cellulose filter (2 lm
pore size; Poretics Co., USA). To the upper compartment 5 104
cells in a 50 lL HBSS (with calcium and magnesium) were added.
Compounds (2.5, 5, and 25 lg/mL) and control were added each in
5695
duplicate following the addition of cells. The chemoattractant buffer (DMSO and HBSS++ without calcium and magnesium, 1:1 ratio)
was added as a control. The chamber was incubated at 37 °C in
CO2 environment for 60 min. Cells within the filter were stained
with hematoxylin and their travelling distance was measured
with the help of light microscope in five randomly chosen fields
per filter.
4.4.6. Cytokine production from mononuclear cells
Cytokine production level was determined by ELISA Kit (Diaclone, Besancon Cedex France) and the results were recorded by
ELISA reader. PBMCs were isolated from heparinized venous blood
of a healthy adult donor by Ficoll gradient centrifugation. Briefly,
the PBMCs were washed and suspended in RPMI-1640 supplemented with 10% AB human serum (Complete culture medium).
Lymphocytes were cultured 105/well in 96-well microtiter plate
in the presence or absence of 1.25 lg/mL PHA. Four different concentrations (16 lg, 4 lg, 1 lg, and 0.5 lg/mL) of each compound
were used in this assay. The plate was incubated for 18 h at
37 °C in a humidified atmosphere of 5% CO2. After 18 h incubation,
the culture plate was kept on ice and the supernatant from each
well was collected carefully into small sterile Eppendorf tube that
was placed on ice. The supernatant was centrifuged for 10 min at
1000g at 4 °C to remove particulate and aggregates. The clear
supernatant from each Eppendorf tube was collected and aliquot
into small volumes and kept at 20 °C till use. The supernatant
was analyzed for IL-2, IL-4, and IFN-c cytokine production.
4.4.7. Statistical analysis
All data are reported as mean ± SD of the mean and the student
t-test was used to determine the difference between test- and control preparations significance was attributed to probability values
p 6 0.05 (p 6 0.005 in some case). The IC50 values were calculated
using Excel-based program.
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