Marhold & Kučera (eds.) • IAPT/IOPB chromosome data 22 TAXON 65 (5) • October 2016: 1200–1207 I O PB CO LU M N Edited by Karol Marhold & Ilse Breitwieser IAPT/IOPB chromosome data 22 Edited by Karol Marhold & Jaromír Kučera Tatyana V. An’kova,1* Maria N. Lomonosova1 & Victor V. Chepinoga2,3 DOI http://dx.doi.org/10.12705/655.40 BORAGINACEAE 1 Central Siberian Botanical Garden SB RAS, Zolotodolinskaya Str. 101, 630090 Novosibirsk, Russia 2 The V.B. Sochava Institute of Geography SB RAS, UlanBatorskaya Str. 1, 664033 Irkutsk, Russia 3 Irkutsk State University, Karl Marx Str. 1, 664003 Irkutsk, Russia * Author for correspondence: ankova_tv@mail.ru All materials CHN; collectors: EN = E.G. Nikolin, ML = M.N. Lomonosova. The reported study was partially supported by Russian Foundation for Basic Research (RFBR), project no. 16-05-00783. Lappula anisacantha (Turcz. ex Bunge) Gürke, 2n = 12; Russia, Sakha (Yakutia) Republic, ML & EN 879 (NS). CAPRIFOLIACEAE Patrinia rupestris (Pall.) Dufr., 2n = 22; Russia, Sakha (Yakutia) Republic, ML & EN 849b (NS). GENTIANACEAE Halenia corniculata (L.) Cornaz, 2n = 22; Russia, Republic of Buryatia, E. Zolotovskaya & E. Gladkikh C168 (IRKU). GERANIACEAE Geranium sibiricum L., 2n = 28; Russia, Sakha (Yakutia) Republic, ML & EN 858 (NS). PLANTAGINACEAE ALLIACEAE Allium ramosum L., 2n = 32; Russia, Sakha (Yakutia) Republic, ML & EN 831a (NS). Allium splendens Willd. ex Schult. & Schult.f., 2n = 16; Russia, Sakha (Yakutia) Republic, ML & EN 798 (NS). APIACEAE Peucedanum puberulum Schischk., 2n = 24; Russia, Sakha (Yakutia) Republic, ML & EN 898 (NS). Seseli condensatum (L.) Rchb.f., 2n = 22; Russia, Sakha (Yakutia) Republic, ML & EN 852 (NS). Sium suave Walter, 2n = 12; Russia, Sakha (Yakutia) Republic, ML & EN 875 (NS). Linaria acutiloba Fisch. ex Rchb., 2n = 12; Russia, Krasnoyarskii Krai, ML 1141h (NS). Linaria genistifolia (L.) Mill., 2n = 12; Russia, Samarskaya Oblast’, ML 1068 (NS). Plantago canescens Adams, 2n = 12; Russia, Sakha (Yakutia) Republic, ML & EN 798 (NS). Veronica longifolia L., 2n = 34; Russia, Krasnoyarskii Krai, ML 1144 (NS). POACEAE Glyceria lithuanica (Gorski) Gorski, 2n = 20; Russia, Republic of Buryatia, A. Konovalov & E. Gladkikh C1570 (IRKU). RANUNCULACEAE ASPARAGACEAE Leptopyrum fumarioides (L.) Rchb., 2n = 14; Russia, Sakha (Yakutia) Republic, ML & EN 790a (NS). ASTERACEAE Viola epipsiloides Á.Löve & D.Löve, 2n = 24; Russia, Republic of Buryatia, V. Chepinoga, A. Konovalov & E. Zolotovskaya C1583 (IRKU). Maianthemum bifolium (L.) F.W.Schmidt, 2n = 36, 54; Russia, Republic of Buryatia, E. Zolotovskaya, E. Gladkikh C1569 (IRKU). VIOLACEAE Artemisia jacutica Drobow, 2n = 18; Russia, Sakha (Yakutia) Republic, ML & EN 871 (NS). Artemisia obtusiloba subsp. martjanovii (Krasch. ex Poljakov) Krasnob., 2n = 42; Russia, Sakha (Yakutia) Republic, ML & EN 849a (NS). All materials for the chromosome column should be submitted electronically to: Karol Marhold, karol.marhold@savba.sk (Institute of Botany, Slovak Academy of Sciences, SK-845 23 Bratislava, Slovakia, and Department of Botany, Charles University, CZ 128-01 Prague, Czech Republic). The full version of this contribution is available in the online edition of TAXON appended to this article. The following citation format is recommended: Baltisberger, M. & Voelger, M. 2006. Sternbergia sicula. In: Marhold, K. (ed.), IAPT/IOPB chromosome data 1. Taxon 55: 444, E2. 1200 Version of Record Marhold & Kučera (eds.) • IAPT/IOPB chromosome data 22 TAXON 65 (5) • October 2016: 1200–1207 Maria A. Farinaccio,1 Andressa Pirolla-Souza,2 João P. Sardin Nasário3 & Eliana R. Forni-Martins3* 1 Universidade Federal de Mato Grosso do Sul (UFMS), Campus Pantanal, Corumbá, MS, Brasil/Programa de Pós-Graduação em Biologia Vegetal, CCBS, UFMS, Campo Grande, MS, Brasil 2 Programa de Pós-Graduação em Biologia Vegetal, CCBS, Universidade Federal de Mato Grosso do Sul, CP 549, CEP 79070-900, Campo Grande, MS, Brasil 3 Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP)/Programa de Pós-Graduação em Biologia Vegetal, IB, UNICAMP, CP 6109, CEP 13083-970, Campinas, Brasil * Author for correspondence: elianafm@unicamp.br All materials CNH; collectors: MAF = Maria Ana Farinaccio, MFF = Mariana Ferrari Felismino; vouchers in CGMS. This study was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq – CASADINHO/PROCAD – Brasil) and Fundação de Apoio ao Desenvolvimento do Ensino, Ciência e Tecnologia do Estado de Mato Grosso do Sul (FUNDECT, Brasil). APOCYNACEAE Araujia odorata (Hook. & Arn.) Fontella & Goyder, 2n = 20; Brazil, Mato Grosso do Sul, MAF & al. 1000. Araujia stormiana Morong, 2n = 20; Brazil, Mato Grosso do Sul, MAF & MFF 917. Araujia stuckertiana (Kurtz ex Heger) Fontella & Goyder, 2n = 20; Brazil, Mato Grosso do Sul, Corumbá, MAF & al. 935. Araujia variegata (Griseb.) Fontella & Goyder, 2n = 20; Brazil, Mato Grosso do Sul, MAF & MFF 910. Aïda Gasmi,1 Laurent Hardion2* & Régine Verlaque3 1 Institut Méditerranéen de Biodiversité et d’Ecologie (IMBE, UMR CNRS 7263), Aix-Marseille Université, Avenue Escadrille Normandie Niemen, Case 461, 13013 Marseille, France 2 Laboratoire Image, Ville, Environnement (LIVE), UMR CNRS, ENGEES, Université de Strasbourg, Institut de Botanique, 28 rue Goethe, 67083 Strasbourg, France 3 Institut Méditerranéen de Biodiversité et d’Ecologie (IMBE, UMR CNRS 7263), Aix-Marseille Université, Case 4, 3 Place Victor Hugo, 13331 Marseille Cedex 3, France * Author for correspondence: hardion@unistra.fr All materials CHN, gathered in situ in SE France, fixed on flowers (F) or seedlings (S) produced from seeds collected by us or by the Conservatoire Botanique National de Corse (CBNC), identified and counted by A. Gasmi, L. Hardion, and R. Verlaque. The present study extends and revises the previous and unpublished results of M. Seidenbinder. Collectors: AG = A. Gasmi, MS = M. Seidenbinder, RV = R. Verlaque, SY = S. Youssef. Vouchers are deposited in MARS. We would like to thank our botanist colleagues P. Donadille, A. Lavagne, P. Moutte and B. Girerd, for their help during field research. FABACEAE Genista corsica (Loisel.) DC., 2n = 42; France, Corsica, RV F95-124. Genista lobelii DC., n = 9, 2n = 18; France, RV F88-01. 2n = 18; France, AG & SY S14, AG & SY S16. Genista pulchella Vis., n = 9, 2n = 18; France, AG F12-01, MS & RV F84-433, MS & RV F84-436, MS & RV F84-438, MS & RV F84-440, MS F87-107, MS F84-429. 2n = 18; France, AG & SY S09-05, MS F84366, AG & SY S09-04, MS F84-397, MS S86-515, MS F87-122, MS F87-123. n = (11), 12, 2n = 22–24; France, MS F83-186, MS F83-187, MS & RV F84-432, MS & RV F84-434, MS & RV F84-437, MS & RV F84-439, MS F87-108, AG S12-02. n = (10), 13, (15), 2n = 27; France, MS F84-371, MS F84-379. n = 18, 2n =36; France, MS F84-518, MS F84-519, MS S84-548, MS S85-169, MS F87-124, MS F87-125, MS F87126, AG & SY F09-02, AG & RV F12-102, AG & RV F12-104, AG & RV F12-105, AG & RV F12-109, AG & RV F12-111, AG & RV F12-113. Genista salzmannii DC. var. salzmannii, 2n = 18; France, Corsica, CBNC-Piazza S11-117, RV F95-104. Genista salzmannii var. lobelioides (Gamisans) Gamisans & Jeanm., 2n = 36; France, Corsica, CBNC-Piazza S11-145. Genista scorpius (L.) DC., n = 20, 2n = ca. 40; France, MS & RV F84-444, MS & RV F84-425, MS F84-266. 2n = ca. 80; France, RV RV F09-115, RV F09-116, RV F09-124. Aleksandr A. Gnutikov,1* Marina V. Protopopova,2 Vasiliy V. Pavlichenko2 & Victor V. Chepinoga3,4 1 The N.I. Vavilov Institute of Plant Genetic Resources (VIR), Bolshaya Morskaya Str. 44, 190000 St.-Petersburg, Russia 2 Siberian Institute of Plant Physiology and Biochemistry SB RAS, Lermontov Str. 132, 664033 Irkutsk, Russia 3 The V.B. Sochava Institute of Geography SB RAS, UlanBatorskaya Str. 1, 664033 Irkutsk, Russia 4 Irkutsk State University, Karl Marx Str. 1, 664003Irkutsk, Russia * Author for correspondence: alexandr2911@yandex.ru All materials CHN; collectors: MP = M.V. Protopopova, VCH = V.V. Chepinoga, VP = V.V. Pavlichenko; vouchers deposited in IRKU. The reported study was partially supported by Russian Foundation for Basic Research (RFBR), projects no. 16-34-60135-mol_a_dk, 14-04-31350-mol_a, 16-05-00783, 14-04-01416, 16-34-01029, 15-0406438. APIACEAE Angelica decurrens (Ledeb.) B.Fedtsch., 2n = 22; Russia, Republic of Buryatia, MP & VP C1525. GENTIANACEAE Swertia baicalensis Popov ex Pissjauk., 2n = 26; Russia, Republic of Buryatia, MP & VP C1527. ONAGRACEAE Epilobium montanum L., 2n = 36; Russia, Republic of Buryatia, MP & VP C1516. PRIMULACEAE Primula pallasii Lehm., 2n = 22; Russia, Irkutskaya Oblast’, MP, VP & VCH C1542. RANUNCULACEAE Anemone altaica Fisch. ex C.A.Mey., 2n = 32; Russia, Republic of Buryatia, MP & VP C1535, MP, VP & VCH C1536; Russia, Irkutskaya Oblast’, MP, VP & VCH C1539, MP, VP & VCH C1543. Anemone baicalensis Turcz. ex Ledeb., 2n = 28; Russia, Republic of Buryatia, MP & VP C1501, MP & VP C1504, MP & VP C1506, MP & VP C1507, MP & VP C1508, MP & VP C1509, MP & VP C1513, MP & VP C1515, MP & VP C1519, MP, VP & VCH C1534; Russia, Irkutskaya Oblast’, MP & VP C1522, MP & VP C1532, MP, VP & VCH C1541, MP, VP & VCH C1548. Caltha palustris L., 2n = 32; Russia, Republic of Buryatia, MP & VP C1503, MP & VP C1505, MP & VP C1520, MP & VP C1523. Eranthis sibirica DC., 2n = 16; Russia, Republic of Buryatia, MP & VP C1511, MP & VP C1517. Version of Record 1201 Marhold & Kučera (eds.) • IAPT/IOPB chromosome data 22 TAXON 65 (5) • October 2016: 1200–1207 Denis A. Krivenko1,2* & Marina O. Burlyaeva3 1 Siberian Institute of Plant Physiology & Biochemistry of the Siberian Branch of the Russian Academy of Sciences, 132 Lermontov Str., 664033 Irkutsk, Russia 2 Irkutsk Scientific Center of the Siberian Branch of the Russian Academy of Sciences, 134 Lermontov Str., 664033 Irkutsk, Russia 3 Federal Research Center the N.I. Vavilov All-Russian Institute of Plant Genetic Resources, 42–44 Bol’shaya Morskaya Str., 190000 St. Petersburg, Russia * Author for correspondence: krivenko.irk@gmail.com All materials CHN; collectors: MOB = Marina O. Burlyaeva; vouchers in IRK (Nelumbo nucifera Gaertn.) and WIR (Vicia L.). The study was supported by the Russian Foundation for Basic Research (research grant no. 15-04-05372а). FABACEAE Vicia ohwiana Hosok., 2n = 12; Russia, Primorskii Krai, MOB 10671, MOB 10672, 14 Oct 2013, MOB s.n. 2n = 24; Russia, Primorskii Krai, MOB 10674. Vicia unijuga A.Braun, 2n = 12; Russia, Irkutskaya Oblast’, MOB 10661. 2n = 24; Russia, Primorskii Krai, MOB 10662, 27 Sep 2011, MOB s.n., A. Sh. Sabitov 10663. Vicia unijuga var. apoda Maxim., 2n = 12; Russia, Primorskii Krai, MOB 10670. 2n = 24; Russia, Primorskii Krai, MOB 10664, MOB 10665, MOB 10666, MOB 10667, MOB 10668, MOB 10669, 9 Oct 2013, MOB s.n., MOB 10673. NELUMBONACEAE Nelumbo nucifera Gaertn. s.l., 2n = 16; Russia, Primorskii Krai, MOB 39873. Tatiana Miháliková,1* Lenka Mártonfiová2 & Iva Hodálová1 1 Institute of Botany, Slovak Academy of Sciences, Dúbravská cesta 9, 845 23 Bratislava, Slovak Republic 2 Pavol Jozef Šafárik University, Botanical Garden, Mánesova 23, 04352 Košice, Slovak Republic * Author for correspondence: tatiana.mihalikova@savba.sk Chromosome numbers counted by L. Mártonfiová; DNA ploidy level ascertained by T. Miháliková; collectors: DRL = D.R. Letz, IH = I. Hodálová, PM = P. Mereďa, TM = T. Miháliková; vouchers are deposited in SAV. This study was supported by the VEGA Agency (project no. VEGA 0008/13). POLYGONACEAE Rumex maritimus L. 2n = 4x = 40, CHN. Slovakia, TM SK45/1. 2n ~ 4x ~ 40, 2C = 2.09–2.22 pg, FCM. Austria, TM AT42/1, TM AT42/2, TM AT42/3, TM AT42/4, TM AT42/5, TM AT42/6, TM AT42/7, TM AT42/8, TM AT42/9, TM AT42/10; Czech Republic, TM CZ35/1, TM CZ35/2, TM CZ35/3, TM CZ35/4, TM CZ35/5, TM CZ35/6, TM CZ35/7, TM CZ35/8, TM CZ37/1, TM CZ37/2, TM CZ37/3, TM CZ37/4, TM CZ37/5, TM CZ37/6, TM CZ37/7, TM CZ37/8, TM CZ37/9, TM CZ37/10; Hungary, TM HU29/1, TM HU29/2, TM HU29/3, TM HU29/4, TM HU29/5, TM HU29/6, TM HU33/1, TM HU33/2, TM HU33/3, TM HU33/4, TM HU33/5, TM HU33/6, TM HU33/7, TM HU33/8, TM HU33/9, TM HU33/10, TM HU33/11, TM HU33/12, TM HU40/1, TM HU40/2, TM HU44/1; Slovakia, TM SK2/1, TM SK2/2, TM SK2/3, TM SK2/4, TM SK2/5, TM SK2/6, TM SK2/7, TM SK2/8, TM SK2/9, 1202 TM SK2/10, TM SK12/1, TM SK12/2, TM SK12/3, TM SK12/4, TM SK12/5, TM SK14/1, TM SK14/2, TM SK14/3, TM SK14/4, TM SK14/5, TM SK14/6, TM SK14/7, TM SK14/8, TM SK14/9, TM SK14/10, TM SK15/1, TM SK15/2, TM SK15/3, TM SK15/4, TM SK15/5, TM SK15/6, TM SK15/7, TM SK15/8, TM SK15/9, TM SK15/10, TM SK17/1, TM SK17/2, TM SK17/3, TM SK17/4, TM SK17/5, TM SK17/6, TM SK17/7, TM SK17/8, TM SK17/9, TM SK17/10, TM SK19/1, TM SK19/2, TM SK19/3, TM SK19/4, TM SK19/5, TM SK19/6, TM SK19/7, TM SK19/8, TM SK19/9, TM SK19/10, TM SK20/1, TM SK20/2, TM SK20/3, TM SK20/4, TM SK20/5, TM SK20/6, TM SK20/7, TM SK20/8, TM SK20/9, TM SK20/10, TM SK22/1, TM SK22/2, TM SK22/3, TM SK22/4, TM SK22/5, TM SK22/6, TM SK22/7, TM SK22/8, TM SK22/9, TM SK23/1, TM SK23/2, TM SK23/3, TM SK23/4, TM SK23/5, TM SK23/6, TM SK23/7, TM SK23/8, TM SK23/9, TM SK23/10, TM SK34/1, TM SK34/2, TM SK34/3, TM SK34/4, TM SK34/5, TM SK34/6, TM SK34/7, TM SK34/8, TM SK34/9; Sweden, IH, PM & DRL SE24/2, IH, PM & DRL SE24/3, IH, PM & DRL SE24/4, IH, PM & DRL SE24/5, IH, PM & DRL SE24/6, IH, PM & DRL SE24/7. Rumex palustris Sm. 2n = 6x = 60, CHN. Slovakia, TM SK46/1. 2n ~ 6x ~ 60, 2C = 3.45–3.65 pg, FCM. Austria, TM AT36/1, TM AT36/2, TM AT36/3, TM AT36/4, TM AT36/5, TM AT36/6, TM AT36/7, TM AT36/8, TM AT36/9, TM AT36/10, TM AT41/1, TM AT41/2, TM AT41/3, TM AT41/4, TM AT41/5, TM AT41/6, TM AT41/7, TM AT41/8, TM AT41/9, TM AT41/10; Czech Republic, TM CZ43/1; Hungary, TM HU26/1, TM HU26/2, TM HU26/3, TM HU26/4, TM HU26/5, TM HU26/6, TM HU26/7, TM HU26/8, TM HU26/9, TM HU26/10, TM HU27/1, TM HU27/2, TM HU27/3 TM HU27/4, TM HU27/5, TM HU27/6, TM HU27/7, TM HU27/8, TM HU27/9, TM HU28/1, TM HU28/2, TM HU28/3, TM HU28/4, TM HU28/5, TM HU28/6, TM HU28/7, TM HU28/8, TM HU28/9, TM HU28/10, TM HU30/1, TM HU30/2, TM HU30/3, TM HU30/4, TM HU30/5, TM HU30/6, TM HU30/7, TM HU30/8, TM HU30/9, TM HU30/10, TM HU31/1, TM HU31/2, TM HU31/3, TM HU31/4, TM HU31/5, TM HU31/6, TM HU31/7, TM HU31/8, TM HU31/9, TM HU32/1, TM HU32/2, TM HU32/3, TM HU32/4, TM HU32/5, TM HU32/6, TM HU32/7, TM HU32/8, TM HU32/9, TM HU32/10, TM HU38/1, TM HU38/2, TM HU38/3, TM HU38/4, TM HU39/1, TM HU39/2, TM HU39/3, TM HU39/4, TM HU39/5, TM HU39/6, TM HU39/7, TM HU39/8, TM HU39/9, TM HU39/10; Slovakia, TM & IH SK1/1, TM & IH SK1/2, TM & IH SK1/3, TM & IH SK1/4, TM & IH SK1/5, TM & IH SK1/6, TM & IH SK1/7, TM & IH SK1/8, TM SK3/1, TM SK3/2, TM SK3/3, TM SK3/4, TM SK3/5, TM SK3/6, TM SK3/7, TM SK3/8, TM SK3/9, TM SK3/10, TM SK4/1, TM SK4/2, TM SK4/3, TM SK4/4, TM SK4/5, TM SK4/6, TM SK4/7, TM SK4/8, TM SK4/9, TM SK4/10, TM SK5/1, TM SK5/2, TM SK5/3, TM SK5/4, TM SK5/5, TM SK5/6, TM SK5/7, TM SK5/8, TM SK5/9, TM SK5/10, TM SK6/1, TM SK6/2, TM SK6/3, TM SK6/4, TM SK6/5, TM SK6/6, TM SK6/7, TM SK6/8, TM SK6/9, TM SK6/10, TM SK7/1, TM SK7/2, TM SK7/3, TM SK7/4, TM SK7/5, TM SK7/6, TM SK7/7, TM SK7/8, TM SK7/9, TM SK7/10, TM & PM SK8/1, TM & PM SK8/2, TM & PM SK8/3, TM & PM SK8/4, TM & PM SK8/5, TM & PM SK8/6, TM & PM SK8/7, TM & PM SK8/8, TM & PM SK8/9, TM & PM SK8/10, TM SK9/1, TM SK9/2, TM SK9/3, TM SK9/4, TM SK9/5, TM SK9/6, TM SK9/7, TM SK9/8, TM SK9/9, TM SK9/10, TM SK10/1, TM SK10/2, TM SK10/3, TM SK10/4, TM SK10/5, TM SK10/6, TM SK10/7, TM SK10/8, TM SK10/9, TM SK10/10, TM SK11/1, TM SK11/2, TM SK11/3, TM SK11/4, TM SK11/5, TM SK11/6, TM SK11/7, TM SK11/8, TM SK11/9, TM SK11/10, TM SK13/1, TM SK13/2, TM SK13/3, TM SK13/4, TM SK13/5, TM SK13/6, TM SK13/7, TM SK13/8, TM SK13/9, TM SK13/10, TM SK16/1, TM SK16/2, TM SK16/3, TM SK16/4, TM SK16/5, TM SK16/6, TM SK16/7, TM SK16/8, TM SK16/9, TM SK16/10, TM SK18/1, TM SK18/2, TM SK18/3, TM SK18/4, TM SK18/5, TM SK18/6, TM SK18/7, TM SK18/8, TM SK18/9, TM SK18/10, TM SK21/1, TM SK21/2, TM SK21/3, TM SK21/4, TM SK21/5, TM SK21/6, TM SK21/7, TM SK21/8, TM SK21/9, TM SK21/10. Version of Record Marhold & Kučera (eds.) • IAPT/IOPB chromosome data 22 TAXON 65 (5) • October 2016: 1200–1207 Spergularia rubra (L.) J.Presl & C.Presl, 2n = 18; Russia, Republic of Buryatia, M.M. Ivanova 12908 (IRK, VLA). Nina S. Probatova,1* Sergey G. Kazanovsky,2 Vyacheslav Yu. Barkalov1 & Vitaly A. Nechaev1 1 Institute of Biology & Soil Science, Far East Branch of the Russian Academy of Sciences, 159 Stoletya Ave., 690022 Vladivostok, Russia 2 Siberian Institute of Plant Physiology & Biochemistry, Siberian Branch of the Russian Academy of Sciences, 132 Lermontova Str., 664033 Irkutsk, Russia * Author for correspondence: probatova@ibss.dvo.ru All materials CHN; collectors: SGK = S.G. Kazanovsky, VAN = V.A. Nechaev, VYB = V.Yu. Barkalov. ALLIACEAE Allium condensatum Turcz., 2n = 16; Russia, Primorskii Krai, VYB 12617 (VLA). CHENOPODIACEAE Teloxys aristata (L.) Moq., 2n = 18; Russia, Irkutskaya Oblast’, M.M. Ivanova 12928 (IRK, VLA). ELAEAGNACEAE Hippophae rhamnoides L., 2n = 24; Russia, Republic of Buryatia, SGK 12910 (IRK, VLA). ERICACEAE Rhododendron aureum Georgi, 2n = 52; Russia, Irkutskaya Oblast’, SGK 12915 (IRK, VLA). Vaccinium myrtillus L., 2n = 24; Russia, Republic of Buryatia, SGK 12920 (IRK, VLA). EUPHORBIACEAE AMARANTHACEAE Amaranthus caudatus L., 2n = 32; Russia, Primorskii Krai, VAN 12887 (VLA). ASPARAGACEAE Polygonatum humile Fisch. ex Maxim., 2n = 20; Russia, Primorskii Krai, VYB 12642 (VLA). ASTERACEAE Ambrosia artemisiifolia L., 2n = 36; Russia, Primorskii Krai, VAN 12949 (VLA). Cirsium vlassovianum Fisch. ex DC., 2n = 28; Russia, Zabaikal’skii Krai, SGK 12524 (IRK, VLA). Grindelia squarrosa (Pursh) Dunal, 2n = 12; Russia, Primorskii Krai, VAN 12885 (VLA). Hieracium korshinskyi Zahn, 2n = 36; Russia, Republic of Buryatia, SGK 12950 (IRK, VLA). Ixeridium gramineum (Fisch.) Tzvelev, 2n = 14; Russia, Republic of Buryatia, SGK 12897 (IRK, VLA). Kalimeris incisa (Fisch.) DC., 2n = 18; Russia, Primorskii Krai, VYB 12633 (VLA). Senecio viscosus L., 2n = 40; Russia, Republic of Buryatia, M.M. Ivanova 12927 (IRK, VLA). Tephroseris kirilowii (Turcz. ex DC.) Holub, 2n = 48; Russia, Primorskii Krai, VAN 12764 (VLA). BRASSICACEAE Brassica elongata subsp. integrifolia (Boiss.) Breistr., 2n = 30; Russia, Irkutskaya Oblast’, A.V. Verkhozina, SGK & O.A. Chernyshova 12898 (IRK, VLA). Camelina microcarpa Andrz. ex DC., 2n = 16; Russia, Republic of Buryatia, SGK 12934 (IRK, VLA). Lepidium apetalum Willd., 2n = 16; Russia, Republic of Buryatia, SGK 12930 (IRK, VLA). CAMPANULACEAE Campanula patula L., 2n = 20; Russia, Republic of Buryatia, SGK 12914 (IRK, VLA). CAPRIFOLIACEAE Patrinia rupestris (Pall.) Dufr., 2n = 22; Russia, Primorskii Krai, VAN 12891 (VLA). Euphorbia lucorum Rupr., 2n = 28; Russia, Primorskii Krai, VYB 12620 (VLA). Euphorbia pseudochamaesyce Fisch. & C.A.Mey., 2n = 22; Russia, Republic of Buryatia, SGK 12933 (IRK, VLA), SGK 12931 (IRK, VLA). FABACEAE Glycyrrhiza pallidiflora Maxim., 2n = 16; Russia, Primorskii Krai, VAN 12924 (VLA). Gueldenstaedtia verna (Georgi) Boriss., 2n = 16; Russia, Primorskii Krai, VAN 12869 (VLA). Podocarpium mandshuricum (Maxim.) Czerep., 2n = 22; Russia, Primorskii Krai, VAN 12810 (VLA). GENTIANACEAE Swertia baicalensis Popov ex Pissjauk., 2n = 32; Russia, Irkutskaya Oblast’, SGK 12916 (IRK, VLA). LAMIACEAE Dracocephalum ruyschiana L., 2n = 14; Russia, Irkutskaya Oblast’, SGK 12911 (IRK, VLA). Leonurus macranthus Maxim., 2n = 20; Russia, Primorskii Krai, VAN 12892 (VLA). LILIACEAE Lilium buschianum Lodd., 2n = 24; Russia, Primorskii Krai, VYB 12625 (VLA). PLANTAGINACEAE Veronica krylovii Schischk., 2n = 18; Russia, Irkutskaya Oblast’, SGK 12905 (IRK, VLA). POACEAE Achnatherum sibiricum (L.) Keng ex Tzvelev, 2n = 24; Russia, Republic of Altai, SGK 12561 (IRK, VLA). Agrostis clavata Trin., 2n = 42; Russia, Primorskii Krai, VYB 12648 (VLA). Calamagrostis angustifolia Kom., 2n = 28; Russia, Primorskii Krai, VAN 12695 (VLA). Poa angustifolia L., 2n = 56; Russia, Primorskii Krai, VAN 12781 (VLA). Spodiopogon sibiricus Trin., 2n = 40; Russia, Primorskii Krai, VYB 12652 (VLA). CARYOPHYLLACEAE Dianthus versicolor Fisch. ex Link, 2n = 30; Russia, Primorskii Krai, VYB 12618 (VLA). Lychnis flos-cuculi L., 2n = 24; Russia, Irkutskaya Oblast’, M.M. Ivanova 12932 (IRK, VLA). POLYGONACEAE Rheum rhabarbarum L., 2n = 44; Russia, Irkutskaya Oblast’, SGK 12907 (IRK, VLA). Version of Record 1203 Marhold & Kučera (eds.) • IAPT/IOPB chromosome data 22 TAXON 65 (5) • October 2016: 1200–1207 RANUNCULACEAE Aquilegia sibirica Lam., 2n = 16; Russia, Irkutskaya Oblast’, SGK & M.M. Ivanova 12917 (IRK, VLA). ROSACEAE Fragaria viridis Weston, 2n = 14; Russia, Republic of Buryatia, SGK 12938 (IRK, VLA). Spiraea alpina Pall., 2n = 18; Russia, Irkutskaya Oblast’, SGK 12900 (IRK, VLA). ASTERACEAE Gnaphalium sylvaticum L., 2n = 56; Russia, Republic of Buryatia, DAK 12857 (IRK, VLA). Hieracium umbellatum agg., 2n = 27; Russia, Irkutskaya Oblast’, DAK 12867 (IRK, VLA). Jacobaea erucifolia (L.) G.Gaertn., B.Mey. & Scherb., 2n = 40; Russia, Republic of Sakha-Yakutia, A.V. Verkhozina & DAK 12566 (IRK, VLA). Saussurea salicifolia (L.) DC., 2n = 26; Russia, Irkutskaya Oblast’, DAK 12834 (IRK, VLA). RUBIACEAE Galium vaillantii DC., 2n = 22; Russia, Irkutskaya Oblast’, M.M. Ivanova 12929 (IRK, VLA). BORAGINACEAE Hackelia deflexa Opiz, 2n = 24; Russia, Republic of Khakassia, ALE 12736 (TK). SOLANACEAE Hyoscyamus niger L., 2n = 34; Russia, Zabaikal’skii Krai, SGK & E.A. Bondarevich 12906 (IRK, VLA); Russia, Primorskii Krai, VAN 12586 (VLA). VIOLACEAE Viola brachysepala Maxim., 2n = 20; Russia, Primorskii Krai, VYB 12645 (VLA). Viola collina Besser, 2n = 20; Russia, Primorskii Krai, VYB 12583 (VLA). Viola extremiorientalis Vorosch. & N.S.Pavlova, 2n = 24; Russia, Primorskii Krai, VYB 12587 (VLA). Viola muehldorfii Kiss, 2n = 24; Russia, Primorskii Krai, VYB 12588 (VLA). Viola primorskajensis (W.Becker) Vorosch., 2n = 24; Russia, Primorskii Krai, VYB 12590 (VLA). Viola woroschilovii Bezd., 2n = 24; Russia, Primorskii Krai, T.A. Bezdeleva 12904 (VLA). BRASSICACEAE Capsella bursa-pastoris (L.) Medik., 2n = 16; Kazakhstan, YuzhnoKazakhstanskaya Oblast’, A.N. Kupriyanov & ALE 12724 (TK). Erucastrum armoracioides Litv., 2n = 30; Kazakhstan, Karagandinskaya Oblast’, ALE 12609 (TK). EUPHORBIACEAE Euphorbia virgata Waldst. & Kit., 2n = 60; Russia, Republic of Buryatia, DAK 12849 (IRK, VLA). FABACEAE Hedysarum turczaninovii Peschkova, 2n = 14; Russia, Republic of Khakassia, ALE 12775 (TK). Oxytropis nuda Basil., 2n = 16 + 0–4B; Russia, Republic of Khakassia, ALE 12752 (TK). Trifolium lupinaster L., 2n = 32; Russia, Republic of Khakassia, ALE 12774 (TK). HYPERICACEAE Nina S. Probatova,1* Denis A. Krivenko2 & Alexandr L. Ebel3 1 Institute of Biology & Soil Science, Far East Branch of the Russian Academy of Sciences, 159 Stoletya Ave., 690022 Vladivostok, Russia 2 Siberian Institute of Plant Physiology & Biochemistry, Siberian Branch of the Russian Academy of Sciences, 132 Lermontova Str., 664033 Irkutsk, Russia 3 National Research Tomsk State University, 36 Lenina Ave., 634050 Tomsk, Russia * Author for correspondence: probatova@ibss.dvo.ru All materials CHN; collectors: ALE = A.L. Ebel, DAK = D.A. Krivenko. ALLIACEAE Allium vodopjanovae N.Friesen, 2n = 16; Russia, Republic of Khakassia, ALE 12750 (TK). AMARANTHACEAE Amaranthus retroflexus L., 2n = 32; Russia, Irkutskaya Oblast’, DAK 12601 (IRK, VLA). Atriplex hortensis L., 2n = 18; Russia, Irkutskaya Oblast’, DAK 12848 (IRK, VLA). Corispermum ulopterum Fenzl ex Ledeb., 2n = 18; Russia, Republic of Buryatia, DAK 12865 (IRK, VLA). ASPARAGACEAE Asparagus schoberioides Kunth, 2n = 20; Russia, Primorskii Krai, DAK 12605 (IRK, VLA). 1204 Hypericum maculatum Crantz, 2n = 16; Russia, Tomskaya Oblast’, ALE 12753 (TK). IRIDACEAE Iris ensata Thunb., 2n = 24 + 0–1B; Russia, Primorskii Krai, DAK 12945 (IRK, VLA). LAMIACEAE Galeopsis bifida Boenn., 2n = 32; Russia, Irkutskaya Oblast’, E.D. Krivenko & DAK 12854 (IRK, VLA). MALVACEAE Hibiscus trionum L., 2n = 56; Russia, Primorskii Krai, E.G. Rudyka 12921 (VLA). ONAGRACEAE Epilobium pseudorubescens A.K.Skvortsov, 2n = 36; Russia, Tomskaya Oblast’, ALE 12734 (TK). PAPAVERACEAE Papaver nudicaule L., 2n = 42; Russia, Irkutskaya Oblast’, DAK 12853 (IRK, VLA). PLANTAGINACEAE Cymbalaria muralis G.Gaertn., B.Mey. & Scherb., 2n = 14; Russia, Primorskii Krai, T.A. Bezdeleva 12902 (VLA). Plantago asiatica L., 2n = 24; Russia, Primorskii Krai, V.P. Verkholat & al. 9047 (VLA). POACEAE Echinochloa colona (L.) Link, 2n = 54; China, Henan Province, DAK 12833 (IRK, VLA). Version of Record Marhold & Kučera (eds.) • IAPT/IOPB chromosome data 22 TAXON 65 (5) • October 2016: 1200–1207 Melica turczaninowiana Ohwi, 2n = 18; Russia, Irkutskaya Oblast’, DAK 12835 (IRK, VLA). Panicum miliaceum L., 2n = 36; Russia, Irkutskaya Oblast’, A.V. Verkhozina 12039 (IRK, VLA). Stipa krylovii Roshev., 2n = 44; Russia, Irkutskaya Oblast’, DAK 12840 (IRK, VLA). POLYGONACEAE Aconogonon ochreatum Nakai, 2n = 20; Russia, Republic of Buryatia, DAK 12851 (IRK, VLA). Rumex gmelinii Turcz., 2n = 40; Russia, Irkutskaya Oblast’, DAK 12839 (IRK, VLA). ASTERACEAE Bidens tripartita L., 2n = 48; Russia, Primorskii Krai, V.A. Nechaev 10783. Brachyactis angusta (Lindl.) Britton, 2n = 14; Russia, Primorskii Krai, V.T. Lapenko 11969. Ixeridium gramineum (Fisch.) Tzvelev, 2n = 14; China, Heilongjiang Province, N.S. Probatova & V.P. Seledets 8950. Leontodon autumnalis L., 2n = 12 + 0–2–3B; Russia, Primorskii Krai, V.T. Lapenko 12846. Phalacroloma strigosum (Muhl. ex Willd.) Tzvelev, 2n = 27; Russia, Primorskii Krai, V.T. Lapenko 11126. Pulicaria vulgaris Gaertn., 2n = 18; Russia, Krasnodarskii Krai, N.S. Probatova & V.P. Seledets 11602. PRIMULACEAE Androsace filiformis Retz., 2n = 20; Russia, Primorskii Krai, V.T. Lapenko 12925 (VLA). BRASSICACEAE Rorippa palustris (L.) Besser, 2n = сa. 32; China, Heilongjiang Province, N.S. Probatova & V.P. Seledets 8153. RANUNCULACEAE Ranunculus sceleratus L., 2n = 32; Russia, Irkutskaya Oblast’, E.V. Pradedova 12863 (IRK, VLA). CARYOPHYLLACEAE Minuartia laricina (L.) Mattf., 2n = 26; Russia, Amurskaya Oblast’, V.P. Verkholat 10386. SCROPHULARIACEAE Scrophularia incisa Weinm., 2n = 48; Russia, Irkutskaya Oblast’, DAK 12852 (IRK, VLA). Scrophularia multicaulis Turcz., 2n = 24; Russia, Republic of Khakassia, ALE 12738 (TK). LILIACEAE Lloydia serotina (L.) Salisb. ex Rchb., 2n = 24; Russia, Kamchatskii Krai, V.P. Verkholat 10224. POACEAE VIOLACEAE Viola hirta L., 2n = 20; Russia, Irkutskaya Oblast’, DAK 12860 (IRK, VLA). Viola philippica Cav., 2n = 48; China, Girin Province, DAK 12936 (IRK, VLA). Viola ruppii All., 2n = 24; Russia, Irkutskaya Oblast’, DAK 12866 (IRK, VLA). 2n = 48; Russia, Irkutskaya Oblast’, DAK 12862 (IRK, VLA). Viola sacchalinensis H.Boissieu, 2n = 20; Russia, Republic of Buryatia, DAK 12858 (IRK, VLA). Nina S. Probatova,1* Vitaly P. Seledets2 & Elvira G. Rudyka1 1 Institute of Biology & Soil Science, Far East Branch of the Russian Academy of Sciences, 159 Stoletya Prospect, 690022 Vladivostok, Russia 2 Pacific Institute of Geography, Far East Branch of the Russian Academy of Sciences, 7 Radio Street, 690041 Vladivostok, Russia * Author for correspondence: probatova@ibss.dvo.ru All materials CHN; vouchers in VLA. ACORACEAE Acorus calamus L., 2n = 24; Russia, Primorskii Krai, V.P. Verkholat & al. 9046. Anisantha tectorum (L.) Nevski, 2n = 14; Russia, Astrakhanskaya Oblast’, N.S. Probatova & V.P. Seledets 11606. Briza media L., 2n = 14; Russia, Leningradskaya Oblast’, N.S. Probatova 4345, N.S. Probatova 4369. 2n = 28; Russia, Leningradskaya Oblast’, N.S. Probatova 4358. Bromopsis australis (Zherebina) Tzvelev & Prob., 2n = 28; Kazakhstan, N.S. Probatova & V.P. Seledets 5133; Kyrgyzstan, N.S. Probatova & V.P. Seledets 5175. Digitaria ciliaris (Retz.) Koeler, 2n = 36; Russia, Krasnodarskii Krai, N.S. Probatova & V.P. Seledets 12343. Elymus woroschilowii Prob., 2n = 42; Russia, Primorskii Krai, E.A. Chubar’ 12427. Elytrigia villosa (Drobow) Tzvelev, 2n = 28; Russia, Republic of Sakha-Yakutia, I.A. Galanina 12872. Eremopyrum triticeum (Gaertn.) Nevski, 2n = 14; Russia, Republic of Daghestan, N.S. Probatova & V.P. Seledets 5560. Leymus interior (Hultén) Tzvelev, 2n = 28; Russia, Republic of SakhaYakutia, N.S. Probatova & V.P. Seledets 3773. Phalaroides arundinacea (L.) Rauschert, 2n = 28; Russia, Kamchatskii Krai, N.S. Probatova & E.G. Rudyka 1940. Poa angustifolia L., 2n = сa. 70; Russia, Leningradskaya Oblast’, A.P. Sokolovskaya 38. Poa lapponica Prokudin, 2n = 28; Russia, Republic of Karelia, N.S. Probatova & V.P. Seledets 11264. Poa skvortzovii Prob., 2n = 42; Russia, Primorskii Krai, N.S. Probatova 5567. Setaria maximowiczii Tzvelev & Prob., 2n = 18; Russia, Primorskii Krai, V.A. Nechaev 8959. ADOXACEAE Adoxa moschatellina L., 2n = 36; Russia, Primorskii Krai, A.E. Kozhevnikov & Z.V. Kozhevnikova 10161. APIACEAE Cnidium dahuricum Fisch. & C.A.Mey., 2n = 20; Russia, Primorskii Krai, Ya.I. Lelikov 7242. POLYGONACEAE Persicaria amphibia (L.) Delarbre, 2n = 66; Russia, Primorskii Krai, V.P. Verkholat & al. 9051. Rumex acetosa L., 2n = 14; Russia, Primorskii Krai, V.P. Verkholat & al. 9053. RANUNCULACEAE ARACEAE Arisaema peninsulae Nakai, 2n = 28; Russia, Primorskii Krai, N.S. Pavlova 10201. Aconitum woroschilovii Luferov (A. maximum auct.: Sokolovskaya, 1963), 2n = 32; Russia, Kamchatskii Krai, A.P. Sokolovskaya 201. Version of Record 1205 Marhold & Kučera (eds.) • IAPT/IOPB chromosome data 22 TAXON 65 (5) • October 2016: 1200–1207 Caltha silvestris Vorosch., 2n = 64; Russia, Primorskii Krai, V.T. Lapenko 12845. ROSACEAE Potentilla paradoxa Nutt., 2n = 28; Russia, Primorskii Krai, N.S. Probatova & V.P. Seledets 8754; China, Heilongjiang Province, N.S. Probatova & V.P. Seledets 8966. Julia V. Shner,1 Tatiana V. Alexeeva,1 Abdullo Kurbonov2 & Michael G. Pimenov1* 1 Botanical Garden, Moscow State University, 119234 Moscow, Russia 2 Botany Department, Tajik State Pedagogical University, Rudaki Av. 121, 733740 Dushanbe, Republic of Tajikistan * Author for correspondence: mgpimenov@mail.ru All material CHN, collected in Tajikistan, Sughd province; collectors: AK = A. Kurbonov, MP = M.G. Pimenov; vouchers in MW. This study was supported by the grant 13-04-00648 from the Russian Foundation for Basic Research (RFBR). APIACEAE Aegopodium tadshikorum Schischk., n = 11; MP & AK 13. Aphanopleura capillifolia (Regel & Schmalh.) Lipsky, 2n = 22; AK 349. Berula erecta (Huds.) Coville, n = 10; MP & AK 48. Carum carvi L., 2n = 20; AK 178, AK 246. Elaeosticta hirtula (Regel & Schmalh.) Kljuykov, Pimenov & V.N. Tikhom., n = 10; MP & AK 36. Eremodaucus lehmannii Bunge, n = 9; MP & AK 49. Ferula karatavica Regel & Schmalh., 2n = 22; AK 375. Ferula karelinii Bunge, n = 11; MP & AK 52. Ferula lithophila Pimenov, n = 11; MP & AK 40. Ferula mogoltavica Lipsky ex Korovin, 2n = 22; AK 296. Ferula penninervis Regel & Schmalh., n = 11; MP & AK 22; 2n = 22; AK 186. Ferula samarkandica Korovin, n = 11; MP & AK 9. Galagania tenuisecta (Regel & Schmalh.) M.G.Vassiljeva & Pimenov, 2n = 22; AK 365. Heracleum lehmannianum Bunge, 2n = 22; AK 171. Oedibasis apiculata (Kar. & Kir.) Koso-Pol., n = 10; MP & AK 62. Pimpinella puberula (DC.) Boiss., n = 10; MP & AK 70. Prangos fedtschenkoi (Regel & Schmalh.) Korovin, n = 11; MP & AK 53. Prangos pabularia Lindl., n = 11; MP & AK 5. Scandix stellata Banks & Sol., 2n = 20; AK 187; AK 120. Schrenkia golickeana (Regel & Schmalh.) B.Fedtsch., n = 11; MP & AK 8, MP & AK 10. Schrenkia vaginata (Ledeb.) Fisch. & C.A.Mey., n = 11; MP & AK 35. Sium medium Fisch. & C.A.Mey., 2n = 12; AK 397. Torilis arvensis (Huds.) Link, n = 6, MP & AK 69; 2n = 12; AK 334. Torilis leptophylla (L.) Rchb.f., 2n = 12; AK 341. Turgenia latifolia (L.) Hoffm., 2n = 18; AK 267. Régine Verlaque,1* Annie Aboucaya,2 Virgile Noble,3,4 Laurent Hardion5 & Bruno Vila1 1 Institut Méditerranéen de Biodiversité et d’Ecologie (IMBE, UMR CNRS 7263), Aix-Marseille Univ., 3 Place V. Hugo, Case 4, 13331 Marseille Cedex 3, France 2 Parc National de Port-Cros, Allée du Castel Sainte-Claire, BP 70220, 83406 Hyères Cedex, France 3 Conservatoire Botanique National Méditerranéen de Porquerolles, 34 Avenue Gambetta, 83400 Hyères, France 4 Institut Méditerranéen de Biodiversité et d’Ecologie (IMBE, UMR CNRS 7263), Aix-Marseille Univ., Europôle Méditerranéen de l’Arbois, 13100 Aix-en-Provence, France 5 Université de Strasbourg. Laboratoire Image, Ville, Environnement (LIVE, UMR 7362 CNRS), Institut de Botanique, 28 rue Goethe, 67083 Strasbourg Cedex, France * Author for correspondence: regine.verlaque@imbe.fr All materials CHN; fixed on flowers (F) or seedlings (S) produced from seeds collected by us or by the Conservatoire Botanique National Méditerranéen (CBNMED), and counted by R. Verlaque and B. Vila; vouchers in MARS. This study was supported by the Parc National de Port-Cros and the CBNMED. The authors are grateful to colleagues who helped us with plant localisation and sampling. AMARANTHACEAE Corispermum gallicum Iljin, 2n = 18; SE France, CBNMED-Noble S13-04. APIACEAE Bupleurum praealtum L., 2n = 16; SE France, Verlaque & Vila F09146. Heracleum pumilum Vill., 2n = 22, n = 11; SE France, Verlaque F79739. APOCYNACEAE Nerium oleander L., 2n = 22; SE France, Hardion S11-140; Greece, Hardion S11-139. ASTERACEAE Hyoseris taurina (Pamp.) Martinoli, 2n = 16; SE France, CBNMEDNoble S11-118. BORAGINACEAE Buglossoides arvensis subsp. permixta (Jord.) R.Fern., 2n = 28; SE France, CBNMED-Noble S14-03. CAMPANULACEAE Solenopsis corsica (Meikle) M.B.Crespo , Serra & Juan, 2n = 28; France, Corsica, Contandriopoulos F66-8. Solenopsis laurentia (L.) C.Presl, 2n = 14; SE France, Aboucaya F1311, Aboucaya F13-12, Aboucaya F13-13; France, Port-Cros Island, Aboucaya F13-10. CARYOPHYLLACEAE Bufonia perennis Pourr., 2n = 16; SE France, Aboucaya F08-12; 2n = 16 + 2B; SE France, Aboucaya F08-11. CAPRIFOLIACEAE Knautia collina (Guérin) Jord., 2n = 20; SE France, Verlaque F09-130. EUPHORBIACEAE Euphorbia davidii Subils, 2n = 56; SE France, Verlaque S10-04. 1206 Version of Record Marhold & Kučera (eds.) • IAPT/IOPB chromosome data 22 TAXON 65 (5) • October 2016: 1200–1207 FABACEAE Vicia dalmatica A.Kern., 2n = 12; SE France, Verlaque F09-127, Verlaque F90-17. Vicia tenuifolia Roth, 2n = 24; SE France, Verlaque F09-128. POACEAE Narduroides salzmanii (Boiss.) Rouy, 2n = 14; SE France, Verlaque F09-118 and F09-123. POLYGALACEAE LILIACEAE Fritillaria involucrata All., 2n = 24; SE France, Noble F11-114. Lilium martagon L., 2n = 24; SE France, Verlaque F09-136. Tulipa agenensis DC., 2n = 24; SE France, Filosa F95-03. ORCHIDACEAE Anacamptis coriophora subsp. fragrans (Pollini) R.M.Bateman, Pridgeon & M.W.Chase, 2n = 38; SE France, Verlaque F09-120. Serapias olbia Verg., 2n = 72; SE France, Aboucaya F11-13. Serapias parviflora Parl., 2n = 36, n = 18; SE France, Aboucaya F11211, Aboucaya F11-212. Serapias strictiflora Welw. ex Veiga, 2n = 72; SE France, Aboucaya F10-25, Aboucaya F10-26, Aboucaya F10-27. Polygala nicaeensis Risso ex W.D.J.Koch subsp. nicaeensis, 2n = 34; SE France, Aboucaya F08-13. SCROPHULARIACEAE Scrophularia canina subsp. pinnatifida var. deschatresii Gamisans, 2n = 24, n = 12; France, N Corsica, Verlaque F95-136. Scrophularia canina subsp. ramosissima (Loisel.) P. Fourn., 2n = 24, n = 12; France, S Corsica, Verlaque S95-103; SE France, Aboucaya F94-27. Scrophularia provincialis Rouy, 2n = 24; SE France, Verlaque & Vila F09-147, CBNMED-Noble F11-115, Verlaque F12-114. Verbascum boerhavii L., 2n = 44–45; SE France, Verlaque & Vila F09-106. Verbascum sinuatum L., 2n = 30; SE France, Aboucaya F09-138. Version of Record 1207 Marhold & Kučera (eds.) • IAPT/IOPB chromosome data 22 TAXON 65 (5) • October 2016: E1–E27 I O PB CO LU M N Edited by Karol Marhold & Ilse Breitwieser IAPT/IOPB chromosome data 22 [extended online version] Edited by Karol Marhold & Jaromír Kučera Tatyana V. An’kova,1* Maria N. Lomonosova1 & Victor V. Chepinoga2,3 ASTERACEAE 1 Central Siberian Botanical Garden SB RAS, Zolotodolinskaya Str. 101, 630090 Novosibirsk, Russia 2 The V.B. Sochava Institute of Geography SB RAS, UlanBatorskaya Str. 1, 664033 Irkutsk, Russia 3 Irkutsk State University, Karl Marx Str. 1, 664003 Irkutsk, Russia * Author for correspondence: ankova_tv@mail.ru * First chromosome count from the given region. The reported study was partially supported by Russian Foundation for Basic Research (RFBR), project no. 16-05-00783. ALLIACEAE Allium ramosum L. * 2n = 32, CHN. Russia, Sakha (Yakutia) Republic, Khangalasskii ulus, 37 km of Pokrovskii tract, Kuldaty river valley, steppe meadow, 61°44′ N, 129°32′ E, 23 Aug 2012, M.N. Lomonosova & E.G. Nikolin 831a (NS) [Fig. 1A]. Allium splendens Willd. ex Schult. & Schult.f. 2n = 16, CHN. Russia, Sakha (Yakutia) Republic, Namskii ulus, northern vicinities of Maimaga village, edge of open birch-spruce woodlands, 63°02′ N, 129°31′ E, 21 Aug 2012, M.N. Lomonosova & E.G. Nikolin 798 (NS) [Fig. 1B]. APIACEAE Peucedanum puberulum Schischk. * 2n = 24, CHN. Russia, Sakha (Yakutia) Republic, between Tulagino and Kapitonovka villages, on way to the Cape Khangalasskii, grass meadow, 61°17′ N, 128°24′ E, 30 Aug 2012, M.N. Lomonosova & E.G. Nikolin 898 (NS) [Fig. 1E]. Seseli condensatum (L.) Rchb.f. * 2n = 22, CHN. Russia, East Siberia, Sakha (Yakutia) Republic, Khangalasskii ulus, vicinities of Ulakhaan-An village, Lena River valley, steppe on the southern slope, 61°17′ N, 128°24′ E, 24 Aug 2012, M.N. Lomonosova & E.G. Nikolin 852 (NS) [Fig. 1D]. Sium suave Walter * 2n = 12, CHN. Russia, Sakha (Yakutia) Republic, MeginoKangalasskii ulus, Kolymskii tract, river bank near the bridge across Suola River, 62°10′ N, 130°38′ E, 28 Aug 2012, M.N. Lomonosova & E.G. Nikolin 875 (NS) [Fig. 1C]. ASPARAGACEAE Maianthemum bifolium (L.) F.W.Schmidt 2n = 36, 54, CHN. Russia, East Siberia, Republic of Buryatia, Kabanskii Raion, foothills of Khamar-Daban Ridge, near mouth of Vydrinaya River, on road side, 469 m alt., 51.48311° N, 104.84990° E, 7 Jul 2015, E. Zolotovskaya & E. Gladkikh C1569 (IRKU). Artemisia jacutica Drobow 2n = 18, CHN. Russia, Sakha (Yakutia) Republic, MeginoKangalasskii ulus, Kolymskii tract, Bestyakh village, sand dunes, 62°10′ N, 130°38′ E, 28 Aug 2012, M.N. Lomonosova & E.G. Nikolin 871 (NS) [Fig. 1F]. Artemisia obtusiloba subsp. martjanovii (Krasch. ex Poljakov) Krasnob. 2n = 42, CHN. Russia, Sakha (Yakutia) Republic, Khangalasskii ulus, vicinities of Elanka village, rocky shore of the Lena River, 61°17′ N, 128°24′ E, 24 Aug 2012, M.N. Lomonosova & E.G. Nikolin 849a (NS) [Fig. 1G]. BORAGINACEAE Lappula anisacantha (Turcz. ex Bunge) Gürke * 2n = 12, CHN. Russia, Sakha (Yakutia) Republic, MeginoKangalasskii ulus, 94th km of Kolymskii tract, road-side, 62°10′ N, 130°38′ E, 21 Aug 2012, M.N. Lomonosova & E.G. Nikolin 879 (NS). CAPRIFOLIACEAE Patrinia rupestris (Pall.) Dufr. * 2n = 22, CHN. Russia, Sakha (Yakutia) Republic, Khangalasskii ulus, vicinities of Elanka village, rocky shore of the Lena River, 61°17′ N, 128°24′ E, 24 Aug 2012, M.N. Lomonosova & E.G. Nikolin 849b (NS) [Fig. 1H]. GENTIANACEAE Halenia corniculata (L.) Cornaz 2n = 22, CHN. Russia, East Siberia, Republic of Buryatia, Kabanskii Raion, foothills of Khamar-Daban Ridge, near mouth of Vydrianaya River, on the forest edge, 464 m alt., 51.48387° N, 104.84861° E, 7 Jul 2015, E. Zolotovskaya & E. Gladkikh C168 (IRKU). GERANIACEAE Geranium sibiricum L. * 2n = 28, CHN. Russia, Sakha (Yakutia) Republic, Khangalasskii ulus, vicinities of Ulakhaan-An village, near the corral, 61°17′ N, 128°24′ E, 24 Aug 2012, M.N. Lomonosova & E.G. Nikolin 858 (NS). PLANTAGINACEAE Linaria acutiloba Fisch. ex Rchb. * 2n = 12, CHN. Russia, Krasnoyarskii Krai, Turukhansk Raion, Turukhansk town, pebble bank of the Yenisei River. 65°47′23.32″ N, 87°58′34.39″ E, 7 Aug 2014, M.N. Lomonosova 1141h (NS). Linaria genistifolia (L.) Mill. * 2n = 12, CHN. Russia, Samarskaya Oblast’, Toglyatti city, territory of the Institute of Ecology of Volga basin RAS, sandy cliff to the Volga River, 23 Sep 2013, M.N. Lomonosova 1068 (NS). Version of Record E1 Marhold & Kučera (eds.) • IAPT/IOPB chromosome data 22 TAXON 65 (5) • October 2016: E1–E27 Plantago canescens Adams 2n = 12, CHN. Russia, Sakha (Yakutia) Republic, Namskii Raion, northern vicinities of Maimaga village, edge of open birch-spruce woodlands, 63°02′ N, 129°31′ E, 31 Aug 2012, M.N. Lomonosova & E.G. Nikolin 798 (NS). Veronica longifolia L. 2n = 34, CHN. Russia, Krasnoyarskii Krai, Turukhanskii Raion, Igarka town, wet meadow along the bank of the Yenisei River, 67°27′00.28″ N 86°31′54.25″ E, 8 Aug 2014, M.N. Lomonosova 1144 (NS). POACEAE Glyceria lithuanica (Gorski) Gorski 2n = 20, CHN. Russia, East Siberia, Republic of Buryatia, Kabanskii Raion, foothills of Khamar-Daban Ridge, between Bolshoi Mamai and Malyi Mamai rivers, bank of the Mamaiskii brook, 507 m alt., 51.44063° N, 104.82035° E, 9 Jul 2015, A. Konovalov & E. Gladkikh C1570 (IRKU). RANUNCULACEAE Leptopyrum fumarioides (L.) Rchb. 2n = 14, CHN. Russia, Sakha (Yakutia) Republic, Namskii ulus, Namskii tract, vicinities of Khamagatta village, abandoned field, 62°40′ N, 129°41′ E, 21 Aug 2012, M.N. Lomonosova & E.G. Nikolin 790a (NS). VIOLACEAE Viola epipsiloides Á.Löve & D.Löve 2n = 24, CHN. Russia, East Siberia, Republic of Buryatia, Kabanskii Raion, foothills of Khamar-Daban Ridge, 2.3 km to the south of Lake Baikal, lower course of Malyi Mamai River, floodplain forest, 504 m alt., 51.44966° N, 104.81711° E, 10 Jul 2015, V. Chepinoga, A. Konovalov & E. Zolotovskaya C1583 (IRKU). Fig. 1. Mitotic metaphase: A, Allium ramosum, 2n = 32; B, Allium splendens, 2n = 16; C, Sium suave, 2n = 12; D, Seseli condensatum, 2n = 22; E, Peucedanum puberulum, 2n = 24; F, Artemisia jacutica, 2n = 18; G, Artemisia obtusiloba subsp. martjanovii, 2n = 42; H, Patrinia rupestris, 2n = 22. ― Scale bars = 5µm. E2 Version of Record Marhold & Kučera (eds.) • IAPT/IOPB chromosome data 22 TAXON 65 (5) • October 2016: E1–E27 Maria A. Farinaccio,1 Andressa Pirolla-Souza,2 João P. Sardin Nasário3 & Eliana R. Forni-Martins3* along roadside, chaco vegetation, 21°42′37.51″ S, 57°53′55.83″ W, 80 m, 2 Jun 2014, M.A. Farinaccio & al. 1000 (CGMS); [Fig. 2A, B]. 1 Universidade Federal de Mato Grosso do Sul (UFMS), Campus Pantanal, Corumbá, MS, Brasil/Programa de Pós-Graduação em Biologia Vegetal, CCBS, UFMS, Campo Grande, MS, Brasil 2 Programa de Pós-Graduação em Biologia Vegetal, CCBS, Universidade Federal de Mato Grosso do Sul, CP 549, CEP 79070-900, Campo Grande, MS, Brasil 3 Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP)/Programa de Pós-Graduação em Biologia Vegetal, IB, UNICAMP, CP 6109, CEP 13083-970, Campinas, Brasil * Author for correspondence: elianafm@unicamp.br Araujia stormiana Morong *+ 2n = 20, CHN. Brazil, Mato Grosso do Sul, Porto Murtinho, along pathway at Fazenda das Flores, chaco vegetation, clay, damp soil, 21°43′06.3″ S, 57°53′53.6″ W, 80 m, 24 Apr 2012, M.A. Farinaccio & M.F. Felismino 917 (CGMS); [Fig. 2C, D). Methods for chromosome counts follow Guerra (1983). * First chromosome count for the species. § The chromosome number here obtained is different from the one reported by other authors (Albers & Meve, 2001). + Endemic to Chaco vegetation. This study was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq – CASADINHO/PROCAD – Brasil) and Fundação de Apoio ao Desenvolvimento do Ensino, Ciência e Tecnologia do Estado de Mato Grosso do Sul (FUNDECT, Brasil). APOCYNACEAE Araujia odorata (Hook. & Arn.) Fontella & Goyder §+ 2n = 20, CHN. Brazil, Mato Grosso do Sul, Porto Murtinho, Araujia stuckertiana (Kurtz ex Heger) Fontella & Goyder *+ 2n = 20, CHN. Brazil, Mato Grosso do Sul, Corumbá, Passo do Lontra, MS-325, along “carandazal” roadside, transition vegetation Chaco-Pantanal, 26 Jun 2012, M.A. Farinaccio & al. 935 (CGMS); [Fig. 3A, B]. Araujia variegata (Griseb.) Fontella & Goyder *+ 2n = 20, CHN. Brazil, Mato Grosso do Sul, Porto Murtinho, BR-267, km 267, along roadside, chaco vegetation, clay soil, 21°44′42.8″ S, 57°29′54.0″ W, 145 m, 23 Apr 2012, M.A. Farinaccio & M.F. Felismino 910 (CGMS); [Fig. 3C, D]. Apocynaceae is an angiosperm family, which occurs in tropical and subtropical regions, and it is less represented in temperate regions (Endress & Bruyns, 2000). This family has 366 genera (Endress & al., 2014), and around 5000 species (Endress, 2004). Many of these species are poisonous and have economic importance, such as wood supply, ornamental and medicinal purposes (Metcalfe & Chalk, 1950; Rizzini & Mors, 1976; Di Stasi & Hiruma-Lima, 2002). Among Asclepiadoideae, one of the biggest subfamilies of Apocynaceae, subordinated to tribe Asclepiadeae and subtribe Fig. 2. Flowers and mitotic chromosomes (2n = 20) of Araujia species: A & B, A. odorata; C & D, A. stormiana. ― Scale bar = 10 µm (for B & D). Version of Record E3 Marhold & Kučera (eds.) • IAPT/IOPB chromosome data 22 TAXON 65 (5) • October 2016: E1–E27 Fig. 3. Flowers and mitotic chromosomes (2n = 20) of Araujia species: A & B, A. stuckertiana, C & D, A. variegata. ― Scale bar = 10 µm (for B & D). Oxypetalinae, is the genus Araujia with 12 species, of which the diversity center is in the Chaco region (Rapini & al., 2011). The genus includes only tendril species with semi-succulent leaves, ovoid fruits and comose seeds. In Asclepiadoideae, the basic chromosome number x = 11 is by far the most predominant number. Among the other base numbers x = 10 is the most frequent, followed by x = 9, and the sporadic x = 12, 13 and 14. A reduction of the karyotype into x = 10 is known only in the advanced tribe Asclepiadeae, where it is important in the Old World subtribe Astephaninae, as well as in the New World subtribes Metastelmatinae and Oxypetalinae (Albers & Meve, 2001). Our counts revealed 2n = 20 for the four species studied. Albers & Meve (2001) also presented 2n = 20 for A. angustifolia (Hook. & Arn.) Steud., but 2n = 22 for A. odorata and A. hortorum E.Fourn. (synonym of Araujia sericifera Brot., type of Araujia). However, two distinct chromosome numbers were previously presented for A. sericifera, 2n = 20 (Schnack & Covas, 1947 sec. Bolkhovskikh & al., 1969; Gadella & al., 1969) and 2n = 22 (Pardi, 1933 sec. Bolkhovskikh & al., 1969). Literature cited Albers, F. & Meve, U. 2001. A karyological survey of Asclepiadoideae, Periplocoideae, and Secamonoideae, and evolutionary considerations within Apocynaceae s.l. Ann. Missouri Bot. Gard. 88: 624–656. http://dx.doi.org/10.2307/3298637 E4 Bolkhovskikh, Z., Grif, V., Matvejeva, T. & Zakharyeva, O. 1969. Chromosome number of flowering plants. Leningrad: Nauka. Di Stasi, L. & Hiruma-Lima, C.A. 2002. Plantas medicinais na Amazônia e na Mata Atlântica, ed. 2. São Paulo: Editora UNESP. Endress, M.E. 2004. Apocynaceae: Brown and now. Telopea 10: 525–541. Endress, M.E. & Bruyns, P.V. 2000. A revised classification of the Apocynaceae s.l. Bot. Rev. (Lancaster) 66: 1–56. http://dx.doi.org/10.1007/BF02857781 Endress, M.E., Liede-Schumann, S. & Meve, U. 2014. An updated classification for Apocynaceae. Phytotaxa 159: 175–194. http://dx.doi.org/10.11646/phytotaxa.159.3.2 Gadella, T.W.J., Kliphuis, E., Lindeman, J.C. & Mennega, E.A. 1969. Chromosome numbers and seedling morphology of some Angiospermae collected in Brazil. Acta Bot. Neerl. 18: 74–83. http://dx.doi.org/10.1111/j.1438-8677.1969.tb00572.x Guerra, M. 1983. O uso de Giemsa na citogenética vegetal – comparação entre a coloração simples e o bandeamento. Ci. Hoje 35: 190–193. Metcalfe, C.R. & Chalk, L. 1950. Anatomy of the dicotyledons, vol. 1. Oxford: Clarendon Press. Rapini, A., Pereira, J.F. & Goyder, D. 2011. Towards a stable generic circumscription in Oxypetalinae (Apocynaceae). Phytotaxa 26: 9–16. http://dx.doi.org/10.11646/phytotaxa.26.1.2 Rizzini, C.T. & Mors, W.B. 1976. Botânica econômica brasileira. São Paulo: EPU e USP. Version of Record Marhold & Kučera (eds.) • IAPT/IOPB chromosome data 22 TAXON 65 (5) • October 2016: E1–E27 Aïda Gasmi,1 Laurent Hardion2* & Régine Verlaque3 1 Institut Méditerranéen de Biodiversité et d’Ecologie (IMBE, UMR CNRS 7263), Aix-Marseille Université, Avenue Escadrille Normandie Niemen, Case 461, 13013 Marseille, France 2 Laboratoire Image, Ville, Environnement (LIVE), UMR CNRS, ENGEES, Université de Strasbourg, Institut de Botanique, 28 rue Goethe, 67083 Strasbourg, France 3 Institut Méditerranéen de Biodiversité et d’Ecologie (IMBE, UMR CNRS 7263), Aix-Marseille Université, Case 4, 3 Place Victor Hugo, 13331 Marseille Cedex 3, France * Author for correspondence: hardion@unistra.fr Materials fixed on flowers (F) or seedlings (S) produced from seeds collected by us or by the Conservatoire Botanique National de Corse (CBNC), identified and counted by A. Gasmi, L. Hardion, and R. Verlaque. The present study completes extends and revises the previous and unpublished results of M. Seidenbinder-Rondon (1989). We would like to thank our botanist colleagues P. Donadille, A. Lavagne, P. Moutte and B. Girerd, for their help during field research. * First chromosome count for the taxon. ** New chromosome number (cytotype) for the taxon. FABACEAE ** Genista corsica (Loisel.) DC. 2n = 42, CHN. France, S Corsica, near Lighthouse Pertusato, Asphodelus shrubland, 60 m, 41°22′03″ N, 09°11′06″ E, 11 May 1995, R. Verlaque F95-124 (MARS 09352); [Fig. 4J]. Genista lobelii DC. n = 9, 2n = 18, CHN. France, Var, Ste Baume Massif, above La Coutronne, rocky ridge, 550 m, 43°20′17″ N, 05°38′42″ E, 20 Jun 1988, R. Verlaque F88-01 (MARS 06731); [Fig. 4A]. 2n = 18, CHN. France, Bouches-du-Rhône, Ste Victoire Massif, rocky ridge, 939 m, 43°32′08″ N, 05°35′02″ E, 29 May 2009, A. Gasmi & S. Youssef S14 (MARS 09168); France, Var, Ste Baume Massif, Le Jouc de l’Aigle, rocky ridge, 1070 m, 43°19′51″ N, 05°46′40″ E, 29 Jun 2009, A. Gasmi & S. Youssef S16 (MARS 03821). Genista pulchella Vis. n = 9, 2n = 18, CHN. France, Var, Rians, pinewood, calcareous soil, 360 m, 43°36′41.8″ N, 05°42′7.9″ E, 2 Jun 2012, A. Gasmi F12-01 (MARS); France, Vaucluse, Mt Ventoux, calcareous rocky slope, 900 m, 44°09′37″ N, 05°11′17″ E, 15 Jun 1984, M. Seidenbinder & R. Verlaque F84-433 (MARS 06720), M. Seidenbinder & R. Verlaque F84-436 (MARS 06719), M. Seidenbinder & R. Verlaque F84-438 (MARS 06724), M. Seidenbinder & R. Verlaque F84-440 (MARS 06723); France, Vaucluse, Mt. Ventoux, Mt. Serein belvedere, calcareous rocky site, 1400 m, 44°11′45″ N, 05°15′31″ E, 5 Jun 1987, M. Seidenbinder F87-107 (MARS 06722); France, Alpes-de-Haute-Provence, Forcalquier, Rocher des Mourres, lavender shrubland, calcareous soil, 715 m, 43°58′43″ N, 05°46′25″ E, 12 Jun 1984, M. Seidenbinder F84-429 (MARS 06721). 2n = 18, CHN. France, Var, near Comps-sur-Artuby, pinewood understory, calcareous soil, 900 m, 43°42′57″ N, 06°34′19″ E, 12 Jun 2009, A. Gasmi & S. Youssef S09-05 (MARS 03825); France, Vaucluse, Luberon Massif, Gros Collet, 900 m, 43°48′89″ N, 05°31′76″ E, 1 Jun 1984, M. Seidenbinder F84-366 (MARS 03826); France, Vaucluse, Luberon Massif, 700 m, 43°49′03.7″ N, 05°32′10.9″ E, 7 Jul 2009, A. Gasmi & S. Youssef S09-04 (MARS); France, Alpes-de-HteProvence, Verdon Gorge, Point-Sublime, cracked rock slab, 800 m, 21 Jun 1987, 43°47′42″ N, 06°23′93″ E, M. Seidenbinder F87-122 (MARS), M. Seidenbinder F87-123 (MARS), 13 Nov 1986, M. Seidenbinder S86-515 (MARS 03591); France, Alpes-de-Hte-Provence, Aubignosc, Gravas, boxwood-lavender shrubland, 564 m, 44°07′13″ N, 05°57′54″ E, 12 Jun 1984, M. Seidenbinder F84-397 (MARS 06725); [Fig. 4B]. ** n = (11), 12, 2n = 22–24, CHN. France, Vaucluse, Mt. Ventoux, between Bédoin and Ramayettes, 1000 m, 44°10′15″ N, 05°12′16″ E, 5 Jun 1983, M. Seidenbinder F83-186 (MARS), M. Seidenbinder F83-187 (MARS); France, Vaucluse, Mt. Ventoux, above Bédouin, calcareous rocky slope, 900 m, 44°09′37″ N, 05°11′17″ E, 15 Jun 1984, M. Seidenbinder & R. Verlaque F84-432 (MARS 06728), M. Seidenbinder & R. Verlaque F84-434 (MARS 06728), M. Seidenbinder & R. Verlaque F84-437 (MARS 06717), M. Seidenbinder & R. Verlaque F84-439 (MARS 06727); France, Vaucluse, Mt. Ventoux, Mt. Serein belvedere, calcareous crest, 1400 m, 44°11′45″ N, 05°15′31″ E, 11 Jun 1987, M. Seidenbinder F87-108 (MARS 06726); France, NW Var, Rians, pinewood, calcareous soil, 360 m, 43°36′41.8″ N, 05°42′07.9″ E, 2 Jun 2012, A. Gasmi S12-02 (MARS); [Fig. 4C]. ** n = (10), 13, (15), 2n = 27, CHN. France, Vaucluse, Luberon Massif, Mourre Nègre, calcareous ridge, 1010 m, 43°49′06″ N, 05°32′05″ E, 1 Jun 1984, M. Seidenbinder F84-371 (MARS 06732); France, Vaucluse, Luberon, Mourre Nègre, calcareous moorland, 850 m, 43°48′45″ N, 05°28′05″ E, 1 Jun 1984, M. Seidenbinder F84-379 (MARS 06733); [Fig. 4D]. ** n = 18, 2n = 36, CHN. France, Alpes-de-Hte-Provence, Verdon Gorge, Moustiers-Ste-Marie, Naverre, calcareous rocky ridge, 1000 m, 43°51′63″ N, 06°15′85″ E, 21 Jun 1987, M. Seidenbinder F87-125 (MARS 06729). 2n = 36, France, Alpes-de-Hte-Provence, Verdon Gorge, between La Palud-sur-Verdon and Châteauneuf-les-Moustiers, calcareous ridge, 1000 m, 43°47′36″ N, 06°20′25″ E, 21 Jun 1987, M. Seidenbinder F87-124 (MARS 09186); France, Alpes-de-Hte-Provence, Verdon Gorge, N Mayreste, calcareous ridge, 1200 m, 43°47′62″ N, 06°16′90″ E, 24 Jun 1984, M. Seidenbinder F84-518 (MARS 06730), M. Seidenbinder F84-519 (MARS 06718); France, Alpes-de-Hte-Provence, Verdon Gorge, pass Plein Voir, calcareous rocky summit, 1050 m, 43°49′73″ N, 06°15′24″ E, 10 Jul 1985, M. Seidenbinder S85-169 (MARS); France, NW Var, near Quinson, dry marly hillock, 370 m, 43°41′44″ N, 06°02′07″ E, 21 Jun 1987, M. Seidenbinder F87-126 (MARS); France, Var, near Aiguines’ old bridge, dry marly hillock, 500 m, 43°48′04” N, 06°14′53″ E, 28 Jun 1984, M. Seidenbinder S84-548 (MARS); France, NW Var, La Verdière, Mourotte, 480 m, 43°38′49″ N, 05°56′44″ E, 31 May 2009, A. Gasmi & S. Youssef F09-02 (MARS); France, NW Var, between Barjols and Montmeyan, Beau Rivet, open habitat on dense marlyplots, 400 m, 43°41′15″ N, 06°02′47″ E, 5 Jun 2012, A. Gasmi & R. Verlaque F12-102 (MARS 09145); France, Var, NW Aups, Moissac, Pélenq, open calcareous habitat, 450 m, 43°37′25″ N, 06°09′27″ E, 5 Jun 2012, A. Gasmi & R. Verlaque F12-104 (MARS 09144), A. Gasmi & R. Verlaque F12-105 (MARS 09143); France, Var, S-W Aups, Collet des Lentilles, red marly, pinewood understory, 693 m, 43°37′10″ N, 06°09′56″ E, 5 Jun 2012, A. Gasmi & R. Verlaque F12-109 (MARS); France, Var, N-E Salernes, near Tourtour, Clappes forest, pinewood understory, 708 m, 43°36′06″ N, 06°20′18″ E, 5 Jun 2012, A. Gasmi & R. Verlaque F12-111 (MARS 09141), A. Gasmi & R. Verlaque F12-113 (MARS 09139); [Fig. 4E]. Genista salzmannii DC. var. salzmannii 2n = 18, CHN. France, N Corsica, Asco, near Bocca Culaghia, siliceous rocky habitats, 1000 m, 42°25′11″ N, 08°57′30.3″ E, Jul 2010, seeds from CBNC-Piazza S11-117 (MARS); France, Corsica, near Ajaccio, Campo dell’Oro, maquis beyond the sand beach, 41°55′31″ N, 08°46′49″ E, 9 May 1995, R. Verlaque F95-104 (MARS 09166); [Fig. 4F]. Genista salzmannii var. lobelioides (Gamisans) Gamisans & Jeanm. * 2n = 36, CHN. France, S Corsica, L’Ospedale, forest, siliceous rocky site, 1000 m, 41°39′46″ N, 09°12′14″ E, seeds from CBNC-Piazza S11-145 (MARS); [Fig. 4G]. Version of Record E5 Marhold & Kučera (eds.) • IAPT/IOPB chromosome data 22 TAXON 65 (5) • October 2016: E1–E27 Genista scorpius (L.) DC. n = 20, 2n = ca. 40, CHN. France, Vaucluse, Mt. Ventoux Massif, Quercus pubescens shrubland, on calcareous soil, 880 m, 44°10′58″ N, 05°11′26″ E, 15 Jun 1984, M. Seidenbinder & R. Verlaque F84-444 (MARS 03596); France, Alpes-de-Hte-Provence, Lure Mountain, near St-Etienne-les-Orgues, edge of Q. pubescens wood, on calcareous soil, 700 m, 44°02′34″ N, 05°48′21″ E, 12 Jun 1984, M. Seidenbinder & R. Verlaque F84-425 (MARS 03598); France, Hérault, near Bel-Air, open forest of evergreen oak, calcareous soil, 150 m, 43°33′41″ N, 03°11′73″ E, 7 May 1984, M. Seidenbinder F84-266 (MARS 03595); [Fig. 4H]. ** 2n = ca. 80, CHN. France, Var, Gapeau Valley, between Signes and Méounes, Rosmarinus shrubland, siliceous rocky site, 500 m, 43°17′92″ N, 05°53′96″ E, 20 May 2009, R. Verlaque F09-115 (MARS 09164), R. Verlaque F09-116 (MARS 09165), R. Verlaque F09-124 (MARS 09167); [Fig. 4I]. The genus Genista sensu Gibbs (1966) contains around a hundred shrub taxa centred in the Mediterranean basin, particularly in Spain (Talavera, 1999). Among the spiny species of Genista subg. Genista, some of French taxa cause taxonomic problems. On windy littoral ridges of SE France (300–1100 m), the endemic G. lobelii forms thorny cushions (10–40 cm). We confirm its stable 2n = 18 (Verlaque, 1992), while closely related taxa are di- and tetraploid in Spain. In G. lobelii, we also found the meiotic anomalies described in Spanish diploids by Sañudo (1979) that “reveal a disequilibrium of genotypes”. Fig. 4. Somatic metaphases (2n) and pollen mitosis (n) : A, Genista lobelii, 2n = 18; B-E, G. pulchella: B, n = 9; C, 2n = 23; D, 2n = 27; E, 2n = 36; F, G. salzmannii var. salzmannii, 2n = 18; G, G. salzmannii var. lobelioides, 2n = 36; H-I, G. scorpius: H, 2n = ca. 40; I, 2n = ca. 80; J, G. corsica, 2n = 42. ― Scale bar = 10 μm. E6 In N-C Mediterranean Massifs (S France, C Italia, Dalmatia: 360–1600 m), G. pulchella shows a high polymorphism linked to its chromosomal variations: 2n = 18, 24, 27, 36 in France. All these cytotypes share specific characters, distinct from those of G. lobelii: branches nearly divaricate (vs. fastigiate), calyx with appressed and patent hairs (vs. short appressed hairs) (Tison & Foucault, 2014). (1) The widespread diploids have a prostrate habit (< 10 cm), woody stems with barely spiny apex, and small hairy leaves with smooth pulvini. Compared to G. lobelii, diploid G. pulchella produces fewer seeds and seedlings (germination < 60%), despite a higher rate of normal pollen (95% vs. < 70%), and a more symmetrical and constant karyotype, with sometimes small satellites (vs. always one or two pairs with large satellites). (2) Few plants with 2n = 22–24 are scattered in Provence and located around prostrate diploid patches. Their main traits are: erect shrub floriferous, spiny pulvini, and strong sterility (80% of abnormal pollen). Given the chromosome numbers of putative progenitors, this form does not match with the hybrid G. × martinii Verg. & Soulié (G. pulchella 2n = 18 × G. scorpius 2n = 40). This cytotype has been found in Italy (Conti, 2007) without G. scorpius, and the same spiny pulvini also appear in Spanish erect tetraploids of the G. lobelii group. In fact, these odd plants could result either from unequal divisions of small pairs of chromosomes, or chromosomal breaks. (3) The few individuals of Mt. Luberon with 2n = 27 are characterized by: long leaves with villous upper surface, elongated pulvini, and high sterility (50%) linked to its triploid level. The latter two cytotypes correspond to abnormal events or accidents, probably without future. (4) In contrast, the 2n = 36 cytotype forms many populations distributed between the Verdon Gorge area and Argens River (300– 1200 m). It differs from the others by: leaves with very short hairs and sericeous young stems, however, its pulvini are similar to those of diploids. These fertile plants (normal pollen > 70%) seem to be autotetraploids rather than allopolyploids between diploids G. lobelii and G. pulchella as suggested by Tison & Foucault (2014), because of their location in CE of the G. pulchella range, far from G. lobelii area. Compared to diploids, all other cytotypes share a rather erect habit (up to 30 cm) and quite spiny stem apex, but have a more limited distribution and higher polymorphism. The proposed intraspecifictreatments seem premature or wrong: on account of nomenclatural problems for the erect G. pulchella subsp. pulchella and the prostrate G. pulchella subsp. villarsii (Clementi) Kerguélen in France (Kerguélen, 1993), or because the polymorphism of French plants includes the three taxa described by Conti (2007): G. pulchella subsp. pulchella (Croatia: low shrub < 12 cm), G. pulchella subsp. villarsiana (Jord.) F.Conti (S France: prostrate < 4 cm, 2n = 24), and G. pulchella subsp. aquilana F.Conti & Manzi (C Italy, Abruzzo: erect shrub up to 27 cm, 2n = 18, 24). In fact, G. pulchella is a polyploid complex in ongoing evolution. Formerly included in G. lobelii (Coste, 1901), the Tyrrhenian endemic G. salzmannii shows high ecological plasticity and polymorphism, linked to its two ploidy levels found in Sardinia (Vila, 1988), and here for the first time in Corsica. The diploid G. salzmannii var. salzmannii differs from the autotetraploid Corsican endemic, G. salzmannii var. lobelioides, by its higher shrubs (0.4–1 m vs. thorny cushions < 0.6 m), living at a lower altitude (0–1200 vs. 1000–2200 m), its flowers mostly born in pairs (vs. single) with shorter pedicels (1–3 vs. 2–5 mm: Jeanmonod & Gamisans, 2007). The aneuploid cytotype of G. scorpius (2n = c. 40), widespread in W Mediterranean (0–1600 m), is a fertile shrub (0.3–2 m), with stout axillary spines. In contrast, the higher ploidy level (2n = ca. 80) is restricted to a single valley in an eastern disjunct area (Var, 500 m), its individuals are smaller, little floriferous, slightly spiny and sterile. In G. corsica (Corsica-Sardinian endemic up to 1 m, 0–1200 m), we have found a chromosome number 2n = 42 that differs from the previous count 2n = 48 (Contandriopoulos, 1962). This new number is more congruent with those of the closely related G. scorpius. Version of Record Marhold & Kučera (eds.) • IAPT/IOPB chromosome data 22 TAXON 65 (5) • October 2016: E1–E27 Phylogenetic study shows that G. scorpius and G. corsica (G. sect. Scorpioides Spach), as G. salzmannii (G. sect. Erinacoides Spach) are well differentiated. In contrast, G. lobelii (sect. Erinacoides) and G. pulchella (G. sect. Spartioides Spach) form an unresolved polytomy (A. Gasmi, unpub.), and seem only separated by chromosomal restructuring. So we confirm the invalidity of sections, while subgenera are supported by molecular data (Pardo & al., 2004). The studied taxa give interesting examples of Genista diversification, in which polyploidy, aneuploidy (followed by basic chromosome number change), but also karyotypic changes have played a major role. While polyploidy always has a high morphological impact (habit, spinescence, size, etc.), it also creates numerous restricted endemic taxa, sometimes sterile. APIACEAE Angelica decurrens (Ledeb.) B.Fedtsch. 2n = 22, CHN. Russia, East Siberia, Republic of Buryatia, Kabanskii Raion, the upper course of the Bolshoi Mamai River, subalpine meadow, near the creek, 1196 m, 51.37783° N, 104.86790° E, 22 Jul 2014, M.V. Protopopova & V.V. Pavlichenko C1525 (IRKU). GENTIANACEAE Swertia baicalensis Popov ex Pissjauk. * 2n = 26, CHN. Russia, East Siberia, Republic of Buryatia, Kabanskii Raion, the upper course of the Bolshoi Mamai River, subalpine meadow, near the creek, 1196 m, 51.37783° N, 104.86790° E, 22 Jul 2014, M.V. Protopopova & V.V. Pavlichenko C1527 (IRKU). Literature cited Contandriopoulos, J. 1962. Recherches sur la flore endémique de Corse et sur ses origines. Thèse. Ann. Fac. Sci. Marseille 32: 1–350. Conti, F. 2007. Morphometric study and taxonomy of Genista pulchella Vis. s.l. (Fabaceae), a south European species. Bot. J. Linn. Soc. 153: 245–254. http://dx.doi.org/10.1111/j.1095-8339.2007.00610.x Coste, H. 1901. Flore descriptive et illustére de la France, de la Corse et des contrées limitrophes, tome 1. Paris: Librairie des Sciences Naturelles. Gibbs, P.E. 1966. Revision of the genus Genista L. Notes Roy. Bot. Gard. Edinburgh 27: 11–99. Jeanmonod, D. & Gamisans, J. 2007. Flora Corsica. Edisud: Aix-en-Provence. Kerguélen, M. 1993. Index synonymique de la flore de France. Paris: Muséum National d’Histoire Naturelle, Secrétariat Faune-Flore. Pardo, C., Cubas, P. & Tahiri, H. 2004. Molecular phylogeny and systematics of Genista (Leguminosae) and related genera based on nucleotide sequences of nrDNA (ITS region) and cpDNA (trnL-trnF intergenic spacer). Pl. Syst. Evol. 244: 93–119. http://dx.doi.org/10.1007/s00606-003-0091-1 Sañudo, A. 1979. Chromosome variability in the Genisteae (Adams.) Benth. (Leguminosae). Webbia 34(1): 363–408. http://dx.doi.org/10.1080/00837792.1979.10670177 Seidenbinder-Rondon, M. 1989. Differenciation et adaptation de certaines Angiospermes en région Méditerranéenne. Ph.D. Thesis, Aix-Marseille University, France. Talavera, S. 1999. Genista. Pp. 45–119 in: Talavera, S., Aedo, C., Castroviejo, S., Romero Zarco, C., Saez, L., Salgueiro, F.J. & Velayos, M. (eds.), Flora iberica, vol. 7(1). Madrid: Real Jardin Botanico, CSIC. Tison, J.-M. & Foucault, B. de 2014. Flora Gallica. Mèze: Édit. Biotope. Verlaque, R. 1992. Modalités de la spéciation chez les Genisteae. Pp. 49–68 in: Conesa i Mor, J.A. & Recasens, J. (eds.), Actes del Simposi Internacional de Botànica “Pius Font i Quer”, vol. 2, Fanerogàmia. Lleida. Vila, R. 1988. Numeri cromosomici per la flora Italiana: 1197–1204. Inform. Bot. Ital. 20: 647–652. Aleksandr A. Gnutikov,1* Marina V. Protopopova,2 Vasiliy V. Pavlichenko2 & Victor V. Chepinoga3,4 1 The N.I. Vavilov Institute of Plant Genetic Resources (VIR), Bolshaya Morskaya Str. 44, 190000 St.-Petersburg, Russia 2 Siberian Institute of Plant Physiology and Biochemistry SB RAS, Lermontov Str. 132, 664033 Irkutsk, Russia 3 The V.B. Sochava Institute of Geography SB RAS, UlanBatorskaya Str. 1, 664033 Irkutsk, Russia 4 Irkutsk State University, Karl Marx Str.1, 664003Irkutsk, Russia * Author for correspondence: alexandr2911@yandex.ru * First chromosome count for the species. ** New chromosome number (cytotype) for the species. The reported study was partially supported by Russian Foundation for Basic Research (RFBR), projects No. 16-34-60135-mol_a_dk, 14-0431350-mol_a, 16-05-00783, 14-04-01416, 16-34-01029, 15-04-06438. ONAGRACEAE Epilobium montanum L. 2n = 36, CHN. Russia, East Siberia, Republic of Buryatia, Kabanskii Raion, the left riverside of the Dulikha River, 0.6 km to the south of Lake Baikal and 0.1 km of the highway M-55, along the cart-road, 460 m, 51.53426° N, 105.02763° E, 20 Jul 2014, M.V. Protopopova & V.V. Pavlichenko C1516 (IRKU). PRIMULACEAE Primula pallasii Lehm. 2n = 22, CHN. Russia, East Siberia, Irkutskaya Oblast’, Slyudyanskii Raion, the lower course of the Khara-Murin River, the right riverside, 4 km to the south of Lake Baikal, 0.45 km of the highway M-55, on the sand, 468 m, 51.45202° N, 104.41242° E, 4 Jun 2015, M.V. Protopopova, V.V. Pavlichenko & V.V. Chepinoga C1542 (IRKU). RANUNCULACEAE Anemone altaica Fisch. ex C.A.Mey. 2n = 32, CHN. Russia, East Siberia, Republic of Buryatia, Kabanskii Raion, the lower course of the Bolshaya Rechka River, the left riverside, 14 km to the east of Lake Baikal, 0.2 km of the highway M-55, floodplain birch forest, 475 m, 51.95793° N, 106.35224° E, 3 Jun 2015, M.V. Protopopova & V.V. Pavlichenko C1535 (IRKU); Russia, East Siberia, Republic of Buryatia, Kabanskii Raion, the lower course of the Mysovka River, the right riverside, 2 km to the south of Lake Baikal, close tothe Babushkin town, aspen and birch forest, 515 m, 51.69365° N, 105.88617° E, 3 Jun 2015, M.V. Protopopova, V.V. Pavlichenko & V.V. Chepinoga C1536 (IRKU); Russia, East Siberia, Irkutskaya Oblast’, Slyudyanskii Raion, the lower course of the Khara-Murin River, the right riverside, 4 km to the south of Lake Baikal, 0.45 km of the highway M-55, thickets of alder, 468 m, 51.45202° N, 104.41242° E, 4 Jun 2015, M.V. Protopopova, V.V. Pavlichenko & V.V. Chepinoga C1539 (IRKU); Russia, East Siberia, Irkutskaya Oblast’, the lower course of the Snezhnaya River, 6.5 km to the south of Lake Baikal, the left riverside, thickets of bird-cherry, 478 m, 51.41756° N, 104.63049° E, 4 Jun 2015, M.V. Protopopova, V.V. Pavlichenko & V.V. Chepinoga C1543 (IRKU). Anemone baicalensis Turcz. ex Ledeb. 2n = 28, CHN. Russia, East Siberia, Republic of Buryatia, Kabanskii Raion, the lower course of the Malyi Mamai River, the left riverside, near the water, 1.5 km to the south of Lake Baikal, 476 m, 51.45226° N, 104.80655° E, 16 Jul 2014, M.V. Protopopova & V.V. Pavlichenko C1501 (IRKU); Russia, East Siberia, Republic of Buryatia, Kabanskii Raion, the right riverside of the Mysovka River, 1.5 km to the south of Lake Baikal, close to the Babushkintown (Mysovaya railway station), near the water, 476 m, 51.70383° N, 105.86507° E, 17 Jul 2014, M.V. Protopopova & V.V. Pavlichenko C1504 (IRKU); Russia, East Siberia, Republic of Buryatia, Kabanskii Raion, the left tributary of the Manturikha River, the left riverside, 0.8 km to the south of Lake Baikal, close to the highway M-55, near the water, 462 m, 51.77146° N, 105.98466° E, 17 Jul 2014, M.V. Protopopova & Version of Record E7 Marhold & Kučera (eds.) • IAPT/IOPB chromosome data 22 TAXON 65 (5) • October 2016: E1–E27 V.V. Pavlichenko C1506 (IRKU); Russia, East Siberia, Republic of Buryatia, Kabanskii Raion, the left riverside of the Bolshaya Telnaya River, 0.3 km to the south of Lake Baikal, close to the highway M-55, near the water, 460 m, 51.72883° N, 105.94135° E, 17 Jul 2014, M.V. Protopopova & V.V. Pavlichenko C1507 (IRKU); Russia, East Siberia, Republic of Buryatia, Kabanskii Raion, the left riverside of the Mishikha River, 1.5 km to the south of Lake Baikal, 0.3 km from the highway M-55, near the water, 459 m, 51.63656° N, 105.54156° E, 17 Jul 2014, M.V. Protopopova & V.V. Pavlichenko C1508 (IRKU); Russia, East Siberia, Republic of Buryatia, Kabanskii Raion, the left riverside of the Vydrinaya River close to the Stantsia Vydrinaya settlement, 1.5 km to the south of Lake Baikal, 0.9 km of the highway M-55, near the water, 457 m, 51.48181° N, 104.85162° E, 18 Jul 2014, M.V. Protopopova & V.V. Pavlichenko C1509 (IRKU); Russia, East Siberia, Republic of Buryatia, Kabanskii Raion, the right tributary of the Bolshoi Mamai River, the right riverside, 3 km to the south of Lake Baikal, 2.5 km of the highway M-55, close to the cart-road, near the water, 520 m, 51.43363° N, 104.79829° E, 19 Jul 2014, M.V. Protopopova & V.V. Pavlichenko C1513 (IRKU); Russia, East Siberia, Republic of Buryatia, Kabanskii Raion, floodplain of the Dulikha River, the left riverside, 0.7 km to the south of Lake Baikal, 0.2 km of the highway M-55, near the water, 474 m, 51.53376° N, 105.02878° E, 20 Jul 2014, M.V. Protopopova & V.V. Pavlichenko C1515 (IRKU); Russia, East Siberia, Republic of Buryatia, Kabanskii Raion, the right riverside of the Bolshoi Mamai River, on the wetland along the stream, 0.3 km to the south of Lake Baikal, 456 m, 51.45546° N, 104.78033° E, 20 Jul 2014, M.V. Protopopova & V.V. Pavlichenko C1519 (IRKU); Russia, East Siberia, Republic of Buryatia, Kabanskii Raion, the lower course of the Bolshaya Rechka River, the left riverside, 14 km to the east of Lake Baikal, 0.2 km of the highway M-55, floodplain birch forest, 475 m, 51.95793° N, 106.35224° E, 3 Jun 2015, M.V. Protopopova, V.V. Pavlichenko & V.V. Chepinoga C1534 (IRKU); Russia, East Siberia, Irkutskaya Oblast’, the left riverside of the Snezhnaya River, close to the highway M-55, 5 km to the south of Lake Baikal, near the water, 474 m, 51.43906° N, 104.63385° E, 20 Jul 2014, M.V. Protopopova & V.V. Pavlichenko C1522 (IRKU); Russia, East Siberia, Irkutskaya Oblast’, Slyudyanskii Raion, the lower course of the Bezimyannaya River, floodplain on the right riverside, 1.4 km to the south of Lake Baikal, birch forest, 461 m, 51.59373° N, 103.90829° E, 30 May 2015, M.V. Protopopova & V.V. Pavlichenko C1532 (IRKU); Russia, East Siberia, Irkutskaya Oblast’, Slyudyanskii Raion, the lower course of the Khara-Murin River, the right riverside, aspen and birch forest, 4 km to the south of Lake Baikal, 0.45 km of the highway M-55, 468 m, 51.45202° N, 104.41242° E, 4 Jun 2015, M.V. Protopopova, V.V. Pavlichenko & V.V. Chepinoga C1541 (IRKU); Russia, East Siberia, Irkutskaya Oblast’, Slyudyanskii Raion, the lower course of the Utulik River, the floodplain poplar forest on the left riverside, 1 km to the south of Lake Baikal, 453 m, 51.54594° N, 104.04675° E, 5 Jun 2015, M.V. Protopopova, V.V. Pavlichenko & V.V. Chepinoga C1548 (IRKU). Caltha palustris L. 2n = 32, CHN. Russia, East Siberia, Republic of Buryatia, Kabanskii Raion, the lower course of the Malyi Mamai River, the left riverside, near the water, 1.5 km to the south of Lake Baikal, 476 m, 51.45226° N, 104.80655° E, 16 Jul 2014, M.V. Protopopova & V.V. Pavlichenko C1503 (IRKU); Russia, East Siberia, Republic of Bur yatia, Kabanskii Raion, the right riverside of the Mysovka River, 1.5 km to the south of Lake Baikal, close to the Babushkin town (Mysovaya railway station), near the water, 476 m, 51.70383° N, 105.86507° E, 17 Jul 2014, M.V. Protopopova & V.V. Pavlichenko C1505 (IRKU); Russia, East Siberia, Republic of Buryatia, Kabanskii Raion, the right riverside of the Bolshoi Mamai River, on the wetland along the stream, 0.3 km to the south of Lake Baikal, 456 m, 51.45546° N, 104.78033° E, 20 Jul 2014, M.V. Protopopova & V.V. Pavlichenko C1520 (IRKU); Russia, East Siberia, Republic of Buryatia, Kabanskii Raion, the upper course of the Bolshoi Mamai River, wet subalpine meadow, E8 1196 m, 51.37783° N, 104.86790° E, 22 Jul 2014, M.V. Protopopova & V.V. Pavlichenko C1523 (IRKU). Eranthis sibirica DC. ** 2n = 16, CHN. Russia, East Siberia, Republic of Buryatia, Kabanskii Raion, the right riverside of the Bolshoi Mamai River, 2 km to the south of Lake Baikal, 1 km of the highway M-55, along the cartroad, 491 m, 51.44234° N, 104.78565° E, 19 Jul 2014, M.V. Protopopova & V.V. Pavlichenko C1511 (IRKU); Russia, East Siberia, Republic of Buryatia, Kabanskii Raion, the left riverside of the Dulikha River, 0.5 km of Lake Baikal, close to the highway M-55, near the stream, 455 m, 51.53479° N, 105.02837° E, 20 Jul 2014, M.V. Protopopova & V.V. Pavlichenko C1517 (IRKU). Denis A. Krivenko1,2* & Marina O. Burlyaeva3 1 Siberian Institute of Plant Physiology & Biochemistry of the Siberian Branch of the Russian Academy of Sciences, 132 Lermontov Str., 664033 Irkutsk, Russia 2 Irkutsk Scientific Center of the Siberian Branch of the Russian Academy of Sciences, 134 Lermontov Str., 664033 Irkutsk, Russia 3 Federal Research Center the N.I. Vavilov All-Russian Institute of Plant Genetic Resources, 42–44 Bol’shaya Morskaya Str., 190000 St. Petersburg, Russia * Author for correspondence: krivenko.irk@gmail.com * New chromosome number (cytotype) for a given taxon. The study was supported by the Russian Foundation for Basic Research (research grant no. 15-04-05372а). FABACEAE Vicia ohwiana Hosok. 2n = 12, CHN. Russia, Primorskii Krai, Vladivostok urban district, Empress Eugenia Archipelago, Peter Great Bay, Russkii Island, Vyatlin Cape, Chernyshev Cove, 0 m, 42°57′52.9″ N, 131°53′58.0″ E, roadside near mixed forest, 17 Oct 2013, M.O. Burlyaeva 10671 (WIR) [Fig. 5a]; Russia, Primorskii Krai, Vladivostok urban district, Empress Eugenia Archipelago, Peter Great Bay, Russkii Island, top of hill, near the fort number 11 Grand Prince Svyatoslav Igorevich Vladivostok Fortress, 79 m, 42°58′29.6″ N, 131°52′52.2″ E, deciduous forest, 17 Oct 2013, M.O. Burlyaeva 10672 (WIR) [Fig. 5b]; Russia, Primorskii Krai, Vladivostok urban district, Empress Eugenia Archipelago, Peter Great Bay, Popov Island, 35 m, 42°57′40.6″N, 131°45′36.5″E, hornbeam forest, 14 Oct 2013, M.O. Burlyaeva s.n. (WIR) [Fig. 5c]. * 2n = 24, CHN. Russia, Primorskii Krai, Vladivostok urban district, Empress Eugenia Archipelago, Peter Great Bay, Popov Island, 35 m, 42°57′40.6″N, 131°45′36.5″E, hornbeam forest, 14 Oct 2013, M.O. Burlyaeva 10674 (WIR) [Fig. 5d]. Vicia unijuga A.Braun 2n = 12, CHN. Russia, Irkutskaya Oblast’, Ust’-Ordynskii Buryatskii Okrug, Bayandaevskii Raion, vicinity of Khandagai village, 677 m, 53°11′ N, 105°48′ E, birch forest, near the road, 5 Aug 2014, M.O. Burlyaeva 10661 (WIR) [Fig. 5e]. 2n = 24, CHN. Russia, Primorskii Krai, Terneiskii Raion, K.G. Abramov Sikhote-Alin State Nature Biosphere Reserve, on the banks of Sukhoi Klyuch River, 95 m, 44°58′16.1″ N, 136°32′03.1″ E, mixed forest (oak, birch, maple, linden, fir, spruce, pine), 9 Oct 2013, M.O. Burlyaeva 10662 (WIR) [Fig. 5f]; Russia, Primorskii Krai, Vladivostok city, vicinity of Far East Experiment Station Federal Research Center the N.I. Vavilov All-Russian Institute of Plant Genetic Resources, 100 m, 43°14′45.1″ N, 132°03′48.4″ E, on the hill in the poplar-oak forest, 27 Sep 2011, M.O. Burlyaeva s.n. (WIR) [Fig. 5g]; Russia, Primorskii Krai, Vladivostok city, vicinity of Far East Version of Record Marhold & Kučera (eds.) • IAPT/IOPB chromosome data 22 TAXON 65 (5) • October 2016: E1–E27 Experiment Station Federal Research Center the N. I. Vavilov AllRussian Institute of Plant Genetic Resources, 100 m, 43°14′45.1″ N, 132°03′48.4″ E, on the hill in the poplar-oak forest, Sep 2012, A.Sh. Sabitov 10663 (WIR) [Fig. 5h]. Vicia unijuga var. apoda Maxim. * 2n = 12, CHN. Russia, Primorskii Krai, Khasanskii Raion, East Manchurian Highland, Gamov Peninsula, road from Vityaz’ village to Astafiev Cove of Peter Great Bay, 104 m, 42°36′52.4″ N, 131°11′68.2″ E, deciduous forest (oak, maples, hazel, kalopanax), 2 Oct 2013, M.O. Burlyaeva 10670 (WIR) [Fig. 5i]. 2n = 24, CHN. Russia, Primorskii Krai, Vladivostok city, vicinity of Far East Experiment Station Federal Research Center the N.I. Vavilov All-Russian Institute of Plant Genetic Resources and vicinity of causeway Bogatoe reservoir, 22 m, 43°14′06.4″ N, 132°04′27.5″ E, slope hill overgrown mixed forest, 25 Sep 2013, M.O. Burlyaeva 10664 (WIR) [Fig. 5j]; Russia, Primorskii Krai, Vladivostok city, vicinity of “Ocean” sanatorium, Pionerskaya Cove of Amur Bay, 2 m, 43°15′51.9″ N, 132°01′13.1″ E, in the larch forest, 28 Sep 2011, M.O. Burlyaeva 10665 (WIR) [Fig. 5k]; Russia, Primorskii Krai, Khasanskii Raion, East Manchurian Highland, Gamov Peninsula, vicinity of Vityaz’ village, 37 m, 42°35′38.3″ N, 131°10′36.4″ E, stony shore sea, 1 Oct 2013, M.O. Burlyaeva 10666 (WIR) [Fig. 5l]; Russia, Primorskii Krai, Khasanskii Raion, East Manchurian Highland, Gamov Peninsula, vicinity of Troitsa Cove of Peter Great, Shul’ts Cape, 26 m, 42°34′48.3″ N, 131°09′54.0″ E, on the path roadside, 1 Oct 2013, M.O. Burlyaeva 10667 (WIR) [Fig. 5m]; Russia, Primorskii Krai, Khasanskii Raion, East Manchurian Highland, Gamov Peninsula, on the hill at rise of Telyakovskii Cove of Peter Great, 44 m, 42°35′02.1″ N, 131°12′31.9″ E, in the deciduous forest with grapes Amur, 1 Oct 2013, M.O. Burlyaeva 10668 (WIR) [Fig. 5n], M.O. Burlyaeva 10669 (WIR) [Fig. 5o]; Russia, Primorskii Krai, Terneiskii Raion, K.G. Abramov Sikhote-Alin State Nature Biosphere Reserve, on the banks of Sukhoi Klyuch River, 95 m, 44°58′16.1″ N, 136°32′03.1″ E, mixed forest (oak, birch, maple, linden, fir, spruce, pine), 9 Oct 2013, M.O. Burlyaeva s.n. (WIR) [Fig. 5p]; Russia, Primorskii Krai, Vladivostok urban district, Empress Eugenia Archipelago, Peter Great Bay, Popov Island, 35 m, 42°57′40.6″ N, 131°45′36.5″ E, undergrowth hornbeam forest, 14 Oct 2013, M.O. Burlyaeva 10673 (WIR) [Fig. 5q]. NELUMBONACEAE Nelumbo nucifera Gaertn. s.l. 2n = 16, CHN. Russia, Primorskii Krai, Chuguevskii Raion, on the way to Dal’negorsk town, between Varfolomeevka village and Varpakhovka village, pool Ussuri River, 44°22′40.29″ N, 134°00′05.82″ E, in water, 26 Aug 2010, M.O. Burlyaeva 39873 (IRK). Fig. 5. Mitotic metaphases. a–c, Vicia ohwiana, 2n = 12; d, V. ohwiana, 2n = 24; e, V. unijuga, 2n = 12; f–h, V. unijuga, 2n = 24; i, V. unijuga var. apoda, 2n = 12; j–q, V. unijuga var. apoda, 2n = 24. ― Scale bar = 1 μm. Version of Record E9 Marhold & Kučera (eds.) • IAPT/IOPB chromosome data 22 TAXON 65 (5) • October 2016: E1–E27 Tatiana Miháliková,1* Lenka Mártonfiová2 & Iva Hodálová1 1 Institute of Botany, Slovak Academy of Sciences, Dúbravská cesta 9, 845 23 Bratislava, Slovak Republic 2 Pavol Jozef Šafárik University, Botanical Garden, Mánesova 23, 04352 Košice, Slovak Republic * Author for correspondence: tatiana.mihalikova@savba.sk This study was supported by the Grant Agency of Ministry of Education of the Slovak Republic and Slovak Academy of Sciences VEGA (grant no. 2/0008/13). . Chromosome numbers counted by L. Mártonfiová; DNA ploidy level ascertained by T. Miháliková. FCM DAPI (4’,6-diamidino-2-phenylindole). The relative DNA genome sizes were estimated using silica-gel dried leaf tissue. Samples of reference plants with known chromosome numbers (2n = 40 and 60) were analysed simultaneously with the internal DNA reference standard (Glycine max (L.) Merr. ‘Polanka’, 2C DNA = 2.50 pg; Doležel & al. 1994), and the ratio of their G0/G1 peak positions was recorded. Then, the DNA ploidy levels of plants of unknown chromosome number were analysed and their peak positions relative to that of the DNA reference standard were assessed. For each measurement, the coefficients of variation (CV) of the standard and the analysed sample were calculated. If the CV of the G0/G1 peak of the sample exceeded the 5% threshold, the analysis was discarded and the sample reanalysed. The sample preparation and FCM procedure followed that of Hodálová & al. (2010). Genome size data were obtained using a CyFlow SL cytometer (Partec, Münster, Germany) equipped with a green solid-state laser (Cobolt Samba 532 nm, 100 mW; Cobolt, Stockholm, Sweden) as the excitation source in the laboratory of flow cytometry at the Institute of Botany, Slovak Academy of Sciences, Bratislava, Slovakia. Direct chromosome counting For karyological analyses, root tip meristems from cuttings or from potted plants were employed. The root tips were pre-treated in a 0.002 M water solution of 8-hydroxyquinoline at 4°C for about 16 h (overnight), fixed in a 1 : 3 mixture of 98% aceticacid and 96% ethanol for 1–24 h, washed in distilled water, macerated in 1N HCl at the temperature of 60°C for 5 min and washed in distilled water. Squashes were made using the cellophane square technique (Murín, 1960). The slides were stained by a 7% solution of Giemsa Stain, Modified Solution, Fluka Analytical, in Sörensen phosphate buffer, dried and observed in a drop of immersion oil using a Leica DM 2500 microscope equipped with camera DFC 290 HD and software Leica application suite v.3.5.0, Switzerland. POLYGONACEAE Rumex maritimus L. The coefficients of variation of G1 peak of R. maritimus sampes and the internal standard ranged from 1.02% to 3.63% (average 2.16%) and from 1.2% to 2.46% (average 1.67%), respectively. All analysed samples were DNA tetraploids with 2n ~ 4x ~ 40. The 2C-values for analysed samples were from 2.09 to 2.22 pg, with highest divergence between two samples 6.22%. 2n = 40, CHN. Slovakia, Záhorská nížina Lowland, Bratislava, WNW of municipal part of Záhorská Bystrica, vicinity of Poľný mlyn Riding Club, field depression, 48°14′45″ N, 17°00′11″ E, 152 m, 6 Jul 2015, T. Miháliková SK45/1 (SAV). 2n ~ 4x ~ 40, 2C = 2.09–2.12 pg, FCM. Austria, Lower Austria, S of village of Baumgarten an der March, Mühlbach Stream, rivulet crossing the road Nr. 49, wet muddy bank and shallow water, 140 m, 48°17′55″ N, 16°52′29″ E, 14 Aug 2013, T. Miháliková AT42/1, E10 T. Miháliková AT42/2, T. Miháliková AT42/3, T. Miháliková AT42/4, T. Miháliková AT42/5, T. Miháliková AT42/6, T. Miháliková AT42/7, T. Miháliková AT42/8, T. Miháliková AT42/9, T. Miháliková AT42/10 (SAV). 2n ~ 4x ~ 40, 2C = 2.13–2.15 pg, FCM. Czech Republic, Milovickovaltická pahorkatina Hills, WNW of village of Sedlec, Nový rybník Pond, wet field depression, 185 m, 48°46′56″ N, 16°40′35″ E, 24 Jul 2013, T. Miháliková CZ35/1, T. Miháliková CZ35/2, T. Miháliková CZ35/3, T. Miháliková CZ35/4, T. Miháliková CZ35/5, T. Miháliková CZ35/6, T. Miháliková CZ35/7, T. Miháliková CZ35/8 (SAV). 2n ~ 4x ~ 40, 2C = 2.12–2.15 pg, FCM. Czech Republic, Dyjskosvratecký úval Valley, SE of village of Kostice, between Morava River and Kyjovka Stream, field depression, 152 m, 48°44′31″ N, 16°59′26″ E, 8 Aug 2013, T. Miháliková CZ37/1, T. Miháliková CZ37/2, T. Miháliková CZ37/3, T. Miháliková CZ37/4, T. Miháliková CZ37/5, T. Miháliková CZ37/6, T. Miháliková CZ37/7, T. Miháliková CZ37/8, T. Miháliková CZ37/9, T. Miháliková CZ37/10 (SAV). 2n ~ 4x ~ 40, 2C = 2.11–2.13 pg, FCM. Hungary, Csongrád County, NNE of village of Ferencszallás, Maros River, alluvium, muddy bank, 83 m, 46°13′20″ N, 20°21′35″ E, 20 Jul 2013, T. Miháliková HU29/1, T. Miháliková HU29/2, T. Miháliková HU29/3, T. Miháliková HU29/4, T. Miháliková HU29/5, T. Miháliková HU29/6 (SAV). 2n ~ 4x ~ 40, 2C = 2.10–2.15 pg, FCM. Hungary, Borsod-AbaújZemplén County, E of village of Tiszalúc, Tisza River, Takta Channel, wet bank, 91 m, 48°02′18″ N, 21°04′44″ E, 21 Jul 2013, T. Miháliková HU33/1, T. Miháliková HU33/2, T. Miháliková HU33/3, T. Miháliková HU33/4, T. Miháliková HU33/5, T. Miháliková HU33/6, T. Miháliková HU33/7, T. Miháliková HU33/8, T. Miháliková HU33/9, T. Miháliková HU33/10, T. Miháliková HU33/11, T. Miháliková HU33/12 (SAV). 2n ~ 4x ~ 40, 2C = 2.15–2.16 pg, FCM. Hungary, Zala County, WSW of village of Zalavár, Kis Balaton Lake, wet bank, 104 m, 46°39′36″ N, 17°07′43″ E, 11 Aug 2013, T. Miháliková HU40/1, T. Miháliková HU40/2 (SAV). 2n ~ 4x ~ 40, 2C = 2.12 pg, FCM. Hungary, Győr-Moson-Sopron County, E of village of Dunaszég, Danube River, bigger lake nearby dead-arm, wet gravelly bank, 110 m, 47°46′09″ N, 17°33′06″ E, 10 Aug 2013, T. Miháliková HU44/1 (SAV). 2n ~ 4x ~ 40, 2C = 2.11–2.16 pg, FCM. Slovakia, Záhorská nížina Lowland, W of village of Zohor, Lósy, wet field depression, 145 m, 48°19′09″ N, 16°57′26″ E, 19 Jul 2011, T. Miháliková SK2/1, T. Miháliková SK2/2, T. Miháliková SK2/3, T. Miháliková SK2/4, T. Miháliková SK2/5, T. Miháliková SK2/6, T. Miháliková SK2/7, T. Miháliková SK2/8, T. Miháliková SK2/9, T. Miháliková SK2/10 (SAV). 2n ~ 4x ~ 40, 2C = 2.12–2.15 pg, FCM. Slovakia, Záhorská nížina Lowland, SE of village of Lozorno, Lozorno Water Reservoir, NE part, denuded muddy bottom, 214 m, 48°19′32″ N, 17°03′56″ E, 27 Jun 2012, T. Miháliková SK12/1, T. Miháliková SK12/2, T. Miháliková SK12/3, T. Miháliková SK12/4, T. Miháliková SK12/5 (SAV). 2n ~ 4x ~ 40, 2C = 2.12–2.15 pg, FCM. Slovakia, Ipeľsko-Rimavská brázda Hills, SE of village of Divín, Ružiná Water Reservoir, NW part, wet ground depression, 252 m, 48°26′33″ N, 19°32′40″ E, 28 Jun 2012, T. Miháliková SK14/1, T. Miháliková SK14/2, T. Miháliková SK14/3, T. Miháliková SK14/4, T. Miháliková SK14/5, T. Miháliková SK14/6, T. Miháliková SK14/7, T. Miháliková SK14/8, T. Miháliková SK14/9, T. Miháliková SK14/10 (SAV). 2n ~ 4x ~ 40, 2C = 2.13–2.16 pg, FCM. Slovakia, IpeľskoRimavská brázda Hills, E of village of Budíkovany, Teplý vrch Water Reservoir, N part, denuded wet bank, 215 m, 48°29′00″ N, 20°05′40″ E, 12 Jul 2012, T. Miháliková SK15/1, T. Miháliková SK15/2, T. Miháliková SK15/3, T. Miháliková SK15/4, T. Miháliková SK15/5, T. Miháliková SK15/6, T. Miháliková SK15/7, T. Miháliková SK15/8, T. Miháliková SK15/9, T. Miháliková SK15/10 (SAV). 2n ~ 4x ~ 40, 2C = 2.11–2.14 pg, FCM. Slovakia, Podunajská nížina Lowland, NNW of village of Boleráz, Boleráz Water Reservoir, S part, denuded wet bank, 184 m, 48°28′56″ N, 17°28′44″ E, 3 Jul 2012, Version of Record Marhold & Kučera (eds.) • IAPT/IOPB chromosome data 22 TAXON 65 (5) • October 2016: E1–E27 T. Miháliková SK17/1, T. Miháliková SK17/2, T. Miháliková SK17/3, T. Miháliková SK17/4, T. Miháliková SK17/5, T. Miháliková SK17/6, T. Miháliková SK17/7, T. Miháliková SK17/8, T. Miháliková SK17/9, T. Miháliková SK17/10 (SAV). 2n ~ 4x ~ 40, 2C = 2.12–2.18 pg, FCM. Slovakia, Košická kotlina Basin, WNW of village of Turňa nad Bodvou, Turniansky rybník Pond, SE part, muddy bank, 184 m, 48°36′04″ N, 20°52′03″ E, 13 Jul 2012, T. Miháliková SK19/1, T. Miháliková SK19/2, T. Miháliková SK19/3, T. Miháliková SK19/4, T. Miháliková SK19/5, T. Miháliková SK19/6, T. Miháliková SK19/7, T. Miháliková SK19/8, T. Miháliková SK19/9, T. Miháliková SK19/10 (SAV). 2n ~ 4x ~ 40, 2C = 2.10–2.17 pg, FCM. Slovakia, Potiská nížina Lowland, NNW of village of Brehov, Ondava River, alluvium, muddy ground depression alongside the embankment, 99 m, 48°30′35″ N, 21°48′44″ E, 13 Jul 2012, T. Miháliková SK20/1, T. Miháliková SK20/2, T. Miháliková SK20/3, T. Miháliková SK20/4, T. Miháliková SK20/5, T. Miháliková SK20/6, T. Miháliková SK20/7, T. Miháliková SK20/8, T. Miháliková SK20/9, T. Miháliková SK20/10 (SAV). 2n ~ 4x ~ 40, 2C = 2.11–2.22 pg, FCM. Slovakia, Potiská nížina Lowland, NE of village of Malý Horeš, Horné Záhumienky, Tica oxbow lake, wet muddy bank, 97 m, 48°24′14″ N, 21°57′16″ E, 14 Jul 2012, T. Miháliková SK22/1, T. Miháliková SK22/2, T. Miháliková SK22/3, T. Miháliková SK22/4, T. Miháliková SK22/5, T. Miháliková SK22/6, T. Miháliková SK22/7, T. Miháliková SK22/8, T. Miháliková SK22/9 (SAV). 2n ~ 4x ~ 40, 2C = 2.10–2.19 pg, FCM. Slovakia, Potiská nížina Lowland, W of village of Leles, vicinity of the Most Sv. Gottharda Bridge, Tica ox-bow lake, denuded muddy bottom, 98 m, 48°28′03″ N, 22°01′03″ E, 14 Jul 2012, T. Miháliková SK23/1, T. Miháliková SK23/2, T. Miháliková SK23/3, T. Miháliková SK23/4, T. Miháliková SK23/5, T. Miháliková SK23/6, T. Miháliková SK23/7, T. Miháliková SK23/8, T. Miháliková SK23/9, T. Miháliková SK23/10 (SAV). 2n ~ 4x ~ 40, 2C = 2.09–2.11 pg, FCM. Slovakia, Záhorská nížina Lowland, WNW of village of Gbely, Adamovské jazero Lake, S part, shallow water and muddy bank, 153 m, 48°43′38″ N, 17°02′11″ E, 24 Jul 2013, T. Miháliková SK34/1, T. Miháliková SK34/2, T. Miháliková SK34/3, T. Miháliková SK34/4, T. Miháliková SK34/5, T. Miháliková SK34/6, T. Miháliková SK34/7, T. Miháliková SK34/8, T. Miháliková SK34/9 (SAV). 2n ~ 4x ~ 40, 2C = 2.14–2.16 pg, FCM. Sweden, Gotland County, NW of village of Klintehamn, Björkhagastrandby, seashore, 0 m, 57°24′16″ N, 18°10′10″ E, 3 Aug 2012, I. Hodálová, P. Mereďa & D.R. Letz SE24/2, I. Hodálová, P. Mereďa & D.R. Letz SE24/3, I. Hodálová, P. Mereďa & D.R. Letz SE24/4, I. Hodálová, P. Mereďa & D.R. Letz SE24/5, I. Hodálová, P. Mereďa & D.R. Letz SE24/6, I. Hodálová, P. Mereďa & D.R. Letz SE24/7 (SAV). Rumex maritimus has been reported to be predominantly tetraploid with 2n = 4x = 40 (e.g., Kihara & Ono, 1926; Chepinoga & al., 2012; Probatova & al., 2012); only one different count (n = 10) originates from south Ural (Russia; Menshikova, 1964). Our reports are the first for the territory of Austria and Hungary. Our reports for populations from Slovakia and Sweden are in agreement with the previous ones published from these countries (cf. Feráková, 1974; Löve, 1942; Lövkvist & Hultgård, 1999). Rumex palustris Sm. The coefficients of variation of G1 peak of R. palustris samples and the internal standard ranged from 1.36% to 3.33% (average 2.06%) and from 1.26% to 2.71% (average 1.74%), respectively. All analysed samples were DNA hexaploids with 2n ~ 6x ~ 60. The 2C-values for analysed samples were from 3.45 to 3.65 pg, with highest divergence between two samples 5.8%. 2n = 60, CHN. Slovakia, Potiská nížina Lowland, E of village of Kaluža, Zemplínska Šírava Water Reservoir, N part, vicinity of Lodenica-boathouse, wet bank, 111 m, 48°48′22″ N, 22°00′41″ E, 3 Jun 2014, T. Miháliková SK46/1 (SAV). 2n ~ 6x ~ 60, 2C = 3.45–3.54 pg, FCM. Austria, Burgenland, SW of village of Sankt Andrä am Zicksee, Baderlacke Lake, SW part, wet bank, 119 m, 47°46′40″ N, 16°56′02″ E, 26 Jul 2013, T. Miháliková AT36/1, T. Miháliková AT36/2, T. Miháliková AT36/3, T. Miháliková AT36/4, T. Miháliková AT36/5, T. Miháliková AT36/6, T. Miháliková AT36/7, T. Miháliková AT36/8, T. Miháliková AT36/9, T. Miháliková AT36/10 (SAV). 2n ~ 6x ~ 60, 2C = 3.50–3.59 pg, FCM. Austria, Burgenland, SW of village of Schützen am Gebirge, pond between Wulka River and Eisbach Rivulet, S part of pond, wet denuded muddy bottom, 127 m, 47°50′24″ N, 16°37′11″ E, 13 Aug 2013, T. Miháliková AT41/1, T. Miháliková AT41/2, T. Miháliková AT41/3, T. Miháliková AT41/4, T. Miháliková AT41/5, T. Miháliková AT41/6, T. Miháliková AT41/7, T. Miháliková AT41/8, T. Miháliková AT41/9, T. Miháliková AT41/10 (SAV). 2n ~ 6x ~ 60, 2C = 3.56 pg, FCM. Czech Republic, Milovickovaltická pahorkatina Hills, WNW of village of Sedlec, Nový rybník Pond, wet field depression, 185 m, 48°46′56″ N, 16°40′35″ E, 24 Jul 2013, T. Miháliková CZ43/1 (SAV). 2n ~ 6x ~ 60, 2C = 3.49–3.54 pg, FCM. Hungary, Veszprém County, SSE of village of Sóly, vicinity of Séd River, wet field margin, 153 m, 47°07′12″ N, 18°02′09″ E, 17 Jul 2013, T. Miháliková HU26/1, T. Miháliková HU26/2, T. Miháliková HU26/3, T. Miháliková HU26/4, T. Miháliková HU26/5, T. Miháliková HU26/6, T. Miháliková HU26/7, T. Miháliková HU26/8, T. Miháliková HU26/9, T. Miháliková HU26/10 (SAV). 2n ~ 6x ~ 60, 2C = 3.48–3.56 pg, FCM. Hungary, Tolna County, town of Dunaföldvár, drainage channel alongside the motorway Nr. 6, wet bank, 105 m, 46°48′34″ N, 18°54′42″ E, 18 Jul 2013, T. Miháliková HU27/1, T. Miháliková HU27/2, T. Miháliková HU27/3, T. Miháliková HU27/4, T. Miháliková HU27/5, T. Miháliková HU27/6, T. Miháliková HU27/7, T. Miháliková HU27/8, T. Miháliková HU27/9 (SAV). 2n ~ 6x ~ 60, 2C = 3.45–3.53 pg, FCM. Hungary, Csongrád County, NNE of village of Ferencszallás, Maros River, alluvium, muddy bank and river terrace, 83 m, 46°13′20″ N, 20°21′35″ E, 20 Jul 2013, T. Miháliková HU28/1, T. Miháliková HU28/2, T. Miháliková HU28/3, T. Miháliková HU28/4, T. Miháliková HU28/5, T. Miháliková HU28/6, T. Miháliková HU28/7, T. Miháliková HU28/8, T. Miháliková HU28/9, T. Miháliková HU28/10 (SAV). 2n ~ 6x ~ 60, 2C = 3.48–3.53 pg, FCM. Hungary, Békés County, village of Doboz, Varga-hosszai-főcsatorna Channel, wet bank, 84 m, 46°44′13″ N, 21°14′57″ E, 20 Jul 2013, T. Miháliková HU30/1, T. Miháliková HU30/2, T. Miháliková HU30/3, T. Miháliková HU30/4, T. Miháliková HU30/5, T. Miháliková HU30/6, T. Miháliková HU30/7, T. Miháliková HU30/8, T. Miháliková HU30/9, T. Miháliková HU30/10 (SAV). 2n ~ 6x ~ 60, 2C = 3.47–3.53 pg, FCM. Hungary, Hajdú-Bihar County, village of Pocsaj, Berettyó River, Ér-főcsatorna Channel, wet bank, 96 m, 47°16′47″ N, 21°48′34″ E, 21 Jul 2013, T. Miháliková HU31/1, T. Miháliková HU31/2, T. Miháliková HU31/3, T. Miháliková HU31/4, T. Miháliková HU31/5, T. Miháliková HU31/6, T. Miháliková HU31/7, T. Miháliková HU31/8, T. Miháliková HU31/9 (SAV). 2n ~ 6x ~ 60, 2C = 3.49–3.53 pg, FCM. Hungary, Borsod-AbaújZemplén County, village of Sajóörös, Sajóörösi-tó Lake, S part, wet bank, 92 m, 47°56′55″ N, 21°00′28″ E, 21 Jul 2013, T. Miháliková HU32/1, T. Miháliková HU32/2, T. Miháliková HU32/3, T. Miháliková HU32/4, T. Miháliková HU32/5, T. Miháliková HU32/6, T. Miháliková HU32/7, T. Miháliková HU32/8, T. Miháliková HU32/9, T. Miháliková HU32/10 (SAV). 2n ~ 6x ~ 60, 2C = 3.50–3.57 pg, FCM. Hungary, Győr-MosonSopron County, E of village of Dunaszég, Danube River, bigger lake nearby dead-arm, wet gravelly bank, 110 m, 47°46′09″ N, 17°33′06″ E, 10 Aug 2013, T. Miháliková HU38/1, T. Miháliková HU38/2, T. Miháliková HU38/3, T. Miháliková HU38/4 (SAV). 2n ~ 6x ~ 60, 2C = 3.51–3.65 pg, FCM. Hungary, KomáromEsztergom County, NE of village of Nagyigmánd, Kocs-Kisigmándi-ér Rivulet, alluvium, wet denuded soil, 121 m, 47°38′56″ N, 18°05′21″ E, 11 Aug 2013, T. Miháliková HU39/1, T. Miháliková HU39/2, Version of Record E11 Marhold & Kučera (eds.) • IAPT/IOPB chromosome data 22 TAXON 65 (5) • October 2016: E1–E27 T. Miháliková HU39/3, T. Miháliková HU39/4, T. Miháliková HU39/5, T. Miháliková HU39/6, T. Miháliková HU39/7, T. Miháliková HU39/8, T. Miháliková HU39/9, T. Miháliková HU39/10 (SAV). 2n ~ 6x ~ 60, 2C = 3.57–3.61 pg, FCM. Slovakia, Podunajská nížina Lowland, Bratislava, W of municipal part of Devínska Nová Ves, Morava River, alluvium, wet soil, 155 m, 48°12′40″ N, 16°58′04″ E, 8 Jul 2011, T. Miháliková & I. Hodálová SK1/1, T. Miháliková & I. Hodálová SK1/2, T. Miháliková & I. Hodálová SK1/3, T. Miháliková & I. Hodálová SK1/4, T. Miháliková & I. Hodálová SK1/5, T. Miháliková & I. Hodálová SK1/6, T. Miháliková & I. Hodálová SK1/7, T. Miháliková & I. Hodálová SK1/8 (SAV). 2n ~ 6x ~ 60, 2C = 3.53–3.62 pg, FCM. Slovakia, Podunajská nížina Lowland, SW of village of Búč, Vojnický potok Stream, wet bank, 116 m, 47°48′05″ N, 18°26′29″ E, 22 Jul 2011, T. Miháliková SK3/1, T. Miháliková SK3/2, T. Miháliková SK3/3, T. Miháliková SK3/4, T. Miháliková SK3/5, T. Miháliková SK3/6, T. Miháliková SK3/7, T. Miháliková SK3/8, T. Miháliková SK3/9, T. Miháliková SK3/10 (SAV). 2n ~ 6x ~ 60, 2C = 3.59–3.64 pg, FCM. Slovakia, Podunajská nížina Lowland, village of Tvrdošovce, small periodical lake nearby the railway station, denuded wet bank, 118 m, 48°05′34″ N, 18°02′31″ E, 22 Jul 2011, T. Miháliková SK4/1, T. Miháliková SK4/2, T. Miháliková SK4/3, T. Miháliková SK4/4, T. Miháliková SK4/5, T. Miháliková SK4/6, T. Miháliková SK4/7, T. Miháliková SK4/8, T. Miháliková SK4/9, T. Miháliková SK4/10 (SAV). 2n ~ 6x ~ 60, 2C = 3.56–3.60 pg, FCM. Slovakia, Podunajská nížina Lowland, E of village of Okánikovo, Poľný kanál Channel, wet bank, 106 m, 47°46′58″ N, 17°53′02″ E, 26 Jul 2011, T. Miháliková SK5/1, T. Miháliková SK5/2, T. Miháliková SK5/3, T. Miháliková SK5/4, T. Miháliková SK5/5, T. Miháliková SK5/6, T. Miháliková SK5/7, T. Miháliková SK5/8, T. Miháliková SK5/9, T. Miháliková SK5/10 (SAV). 2n ~ 6x ~ 60, 2C = 3.54–3.59 pg, FCM. Slovakia, Záhorská nížina Lowland, SE of village of Borský Svätý Jur, Horná studená voda Water Reservoir, W part, wet bank alongside the embankment, 171 m, 48°35′09″ N, 17°04′44″ E, 12 Aug 2011, T. Miháliková SK6/1, T. Miháliková SK6/2, T. Miháliková SK6/3, T. Miháliková SK6/4, T. Miháliková SK6/5, T. Miháliková SK6/6, T. Miháliková SK6/7, T. Miháliková SK6/8, T. Miháliková SK6/9, T. Miháliková SK6/10 (SAV). 2n ~ 6x ~ 60, 2C = 3.51–3.64 pg, FCM. Slovakia, Podunajská nížina Lowland, Bratislava, NE of municipal part of Vajnory, Struha Stream, wet denuded bank, 130 m, 48°12′52″ N, 17°12′53″ E, 22 Aug 2011, T. Miháliková SK7/1, T. Miháliková SK7/2, T. Miháliková SK7/3, T. Miháliková SK7/4, T. Miháliková SK7/5, T. Miháliková SK7/6, T. Miháliková SK7/7, T. Miháliková SK7/8, T. Miháliková SK7/9, T. Miháliková SK7/10 (SAV). 2n ~ 6x ~ 60, 2C = 3.55–3.63 pg, FCM. Slovakia, Podunajská nížina Lowland, ENE of town of Galanta, Kráľová Water Reservoir, W part nearby Kaskády Event Hotel, denuded wet bank, 119 m, 48°12′27″ N, 17°48′04″ E, 19 Jun 2012, T. Miháliková & P. Mereďa SK8/1, T. Miháliková & P. Mereďa SK8/2, T. Miháliková & P. Mereďa SK8/3, T. Miháliková & P. Mereďa SK8/4, T. Miháliková & P. Mereďa SK8/5, T. Miháliková & P. Mereďa SK8/6, T. Miháliková & P. Mereďa SK8/7, T. Miháliková & P. Mereďa SK8/8, T. Miháliková & P. Mereďa SK8/9, T. Miháliková & P. Mereďa SK8/10 (SAV). 2n ~ 6x ~ 60, 2C = 3.52–3.62 pg, FCM. Slovakia, Podunajská nížina Lowland, E of town of Komárno, Váh River, water channel alongside the embankment, shallow water, 106 m, 47°46′04″ N, 18°08′21″ E, 22 Jun 2012, T. Miháliková SK9/1, T. Miháliková SK9/2, T. Miháliková SK9/3, T. Miháliková SK9/4, T. Miháliková SK9/5, T. Miháliková SK9/6, T. Miháliková SK9/7, T. Miháliková SK9/8, T. Miháliková SK9/9, T. Miháliková SK9/10 (SAV). 2n ~ 6x ~ 60, 2C = 3.49–3.60 pg, FCM. Slovakia, Podunajská nížina Lowland, village of Komjatice, Nitra River, alluvium, denuded muddy soil, 126 m, 48°09′06″ N, 18°10′37″ E, 26 Jun 2012, T. Miháliková SK10/1, T. Miháliková SK10/2, T. Miháliková SK10/3, T. Miháliková SK10/4, T. Miháliková SK10/5, T. Miháliková SK10/6, E12 T. Miháliková SK10/7, T. Miháliková SK10/8, T. Miháliková SK10/9, T. Miháliková SK10/10 (SAV). 2n ~ 6x ~ 60, 2C = 3.55–3.60 pg, FCM. Slovakia, Podunajská nížina Lowland, NW of town of Vráble, Vráble Water Reservoir, S part, wet bank, 147 m, 48°15′23″ N, 18°17′45″ E, 26 Jun 2012, T. Miháliková SK11/1, T. Miháliková SK11/2, T. Miháliková SK11/3, T. Miháliková SK11/4, T. Miháliková SK11/5, T. Miháliková SK11/6, T. Miháliková SK11/7, T. Miháliková SK11/8, T. Miháliková SK11/9, T. Miháliková SK11/10 (SAV). 2n ~ 6x ~ 60, 2C = 3.52–3.59 pg, FCM. Slovakia, Ipeľskorimavská brázda Hills, E of village of Ipeľské Predmostie, Ipeľské hony Nature Reserve, Ipeľ River, alluvium, wet denuded soil, 128 m, 48°03′52″ N, 19°03′43″ E, 28 Jun 2012, T. Miháliková SK13/1, T. Miháliková SK13/2, T. Miháliková SK13/3, T. Miháliková SK13/4, T. Miháliková SK13/5, T. Miháliková SK13/6, T. Miháliková SK13/7, T. Miháliková SK13/8, T. Miháliková SK13/9, T. Miháliková SK13/10 (SAV). 2n ~ 6x ~ 60, 2C = 3.49–3.53 pg, FCM. Slovakia, Podunajská nížina Lowland, NE of town of Nové Mesto nad Váhom, Zelená voda Lake, wet muddy bank, 178 m, 48°46′08″ N, 17°51′48″ E, 3 Jul 2012, T. Miháliková SK16/1, T. Miháliková SK16/2, T. Miháliková SK16/3, T. Miháliková SK16/4, T. Miháliková SK16/5, T. Miháliková SK16/6, T. Miháliková SK16/7, T. Miháliková SK16/8, T. Miháliková SK16/9, T. Miháliková SK16/10 (SAV). 2n ~ 6x ~ 60, 2C = 3.53–3.58 pg, FCM. Slovakia, Ipeľsko rimavská brázda Hills, SE of village of Hubovo, Hubovo Water Reservoir, S part, wet muddy bank, 220 m, 48°23′22″ N, 20°22′48″ E, 12 Jul 2012, T. Miháliková SK18/1, T. Miháliková SK18/2, T. Miháliková SK18/3, T. Miháliková SK18/4, T. Miháliková SK18/5, T. Miháliková SK18/6, T. Miháliková SK18/7, T. Miháliková SK18/8, T. Miháliková SK18/9, T. Miháliková SK18/10 (SAV). 2n ~ 6x ~ 60, 2C = 3.52–3.61 pg, FCM. Slovakia, Potiská nížina Lowland, NNW of village of Brehov, Ondava River, alluvium, muddy ground depression alongside the embankment, 99 m, 48°30′35″ N, 21°48′44″ E, 13 Jul 2012, T. Miháliková SK21/1, T. Miháliková SK21/2, T. Miháliková SK21/3, T. Miháliková SK21/4, T. Miháliková SK21/5, T. Miháliková SK21/6, T. Miháliková SK21/7, T. Miháliková SK21/8, T. Miháliková SK21/9, T. Miháliková SK21/10 (SAV). The previous chromosome reports gave two ploidy levels for R. palustris: 2n = 4x = 40 from different botanical gardens (material of unknown origin, e.g., Jaretzky, 1928; Degraeve, 1975) and from Scandinavia and Bulgaria (e.g., Löve, 1942; Raycheva, 2005) and 2n = 6x = 60 from Denmark, Greece, Spain and United Kingdom (e.g., Ichikawa & al., 1971; Strid & Andersson, 1985; García & al., 1989; Montgomery & al., 1997). Our data are the first ones given for R. palustris from the Czech Republic and Slovakia; those from Austria and Hungary corroborate the earlier ones given for his country (Degraeve, 1975; Dobeš & al., 1996). Literature cited Chepinoga, V.V., Gnutikov, A.A., Lubogoschinsky, P.I. & Fleckenstein, K.M. 2012. [Reports] in: Marhold, K. (ed.), IAPT/IOPB chromosome data 13. Taxon 61: 889–891, E5–E10. http://www.iopb.org/PDF/IAPT_IOPB_Chr_data13.pdf Degraeve, N. 1975. Contribution a l’etude cytotaxonomique des Rumex. I. Le Genre Rumex L. sensu stricto. Caryologia 28: 187–201. http://dx.doi.org/10.1080/00087114.1975.10796609 Dobeš, C., Kiehn, M. & Vitek, E. 1996. Beiträge zur Gefässpflanzen-Flora von Österreich: Chromosomenzählungen III. Verh. Zool.-Bot. Ges. Wien 133: 301–318. Doležel, J., Doleželová, M. & Novák, F.J. 1994. Flow cytometric estimation of nuclear DNA mount in diploid bananas (Musa acuminata and M. balbisiana). Biol. Pl. 36: 351–357. Feráková, V. 1974. [Report]. In: Májovský & al., Index of chromosome numbers of Slovakian flora (Part 3). Acta Fac. Rerum Nat. Univ. Comen., Bot. 22: 15. García, C., Pastor, J. & Luque, T. 1989. Contribución al estudio cariológico del género Rumex (Polygonaceae). Acta Bot. Malac. 14: 129–140. Version of Record Marhold & Kučera (eds.) • IAPT/IOPB chromosome data 22 TAXON 65 (5) • October 2016: E1–E27 Hodálová, I., Mereďa, P., Jr., Vinikarová, A., Grulich, V. & Rotreklová, O. 2010. A new cytotype of Jacobaea vulgaris (Asteraceae): Frequency, morphology and origin. Nordic J. Bot. 28: 413–427. Ichikawa, S., Sparrow, A.H., Frankton, C., Nauman, A.F., Smiths, E.B. & Pond, V. 1971. Chromosome number, volume and nuclear volume relationships in a polyploid series (2x–20x) of the genus Rumex. Canad. J. Genet. Cytol. 13: 842–863. Jaretzky, R. 1928. Histologische und karyologische Studien an Polygonaceae. Jahrb. Wiss. Bot. 69: 357–490. Kihara, H. & Ono, T. 1926. Chromosomenzahlen und systematische Gruppierung der Rumex-Arten. Z. Zellf. Mikroskop. Anat. 4: 475–481. http://dx.doi.org/10.1007/BF00391215 Löve, A. 1942. Cytogenetic studies in Rumex. III. Some notes on the Scandinavian species of the genus. Hereditas (Lund) 28: 289–296. http://dx.doi.org/10.1111/j.1601-5223.1942.tb03281.x Lövkvist, B. & Hultgård, U.M. 1999. Chromosome numbers in south Swedish vascular plants. Opera Bot. 137: 1–42. Menshikova, E.A. 1964. Anatomicheskaya i kariologicheskaya kharakteristika semeistva grechishnykh. Avtoref. Kand. Diss. Permskii Gos. Univ., Perm [Anatomical and caryological characteristics of the Buckwheat family. Ph.D. Thesis, Perm.] (in Russian). http://dlib.rsl.ru/viewer/01000787819#?page=1 Montgomery, L., Khalaf, M., Bailey, J.P. & Gornal, K.J. 1997. Contributions to a cytological catalogue of the British and Irish flora, 5. Watsonia 21: 365–368. Murín, A. 1960. Substitution of cellophane for glass covers to facilitate preparation of permanent squashes and smears. Stain Technol. 35: 351–353. Probatova, N.S., Kazanovsky, S.G., Shatokhina, A.V., Rudyka, E.G., Verkhozina, A.V. & Krivenko, D.A. 2012. [Reports] in: Marhold, K. (ed.), IAPT/IOPB chromosome data 14. Taxon 61: 1342–1344, E23–E28. http://www.iopb.org/PDF/IAPT_IOPB_Chr_data14.pdf Raycheva, T. 2005. Chromosome counts in some Bulgarian taxa of Rumex L., subgenus Rumex (Polygonaceae). Pp. 397–408 in: Gruev, B., Nikolova, M. & Donev, A. (eds.), Proceedings of the Balkan scientific conference of biology in Plovdiv (Bulgaria) from 19th till 21st of May 2005. Plovdiv: Plovdiv University Press. Strid, A. & Andersson, I. A. 1985. Chromosome numbers of Greek mountain plants. An annotated list of 115 species. Bot. Jahrb. Syst. 107: 203–228. Nina S. Probatova,1* Sergey G. Kazanovsky,2 Vyacheslav Yu. Barkalov1 & Vitaly A. Nechaev1 1 Institute of Biology & Soil Science, Far East Branch of the Russian Academy of Sciences, 159 Stoletya Ave., 690022 Vladivostok, Russia 2 Siberian Institute of Plant Physiology & Biochemistry, Siberian Branch of the Russian Academy of Sciences, 132 Lermontova Str., 664033 Irkutsk, Russia * Author for correspondence: probatova@ibss.dvo.ru * First chromosome count for the species. ** New chromosome number (cytotype) for the species. ALLIACEAE Allium condensatum Turcz. 2n = 16, CHN. Russia, Far East, Primorskii Krai, Khassanskii Raion, the Reid Pallady Bay, Mramornaya Mt., on the rocks, 16 Jun 2014, V.Yu. Barkalov 12617 (VLA). AMARANTHACEAE Amaranthus caudatus L. 2n = 32, CHN. Russia, Far East, Primorskii Krai, Vladivostok city, Kirova Str., grassy slope, 19 Sep 2015, V.A. Nechaev 12887 (VLA). ASPARAGACEAE Polygonatum humile Fisch. ex Maxim. 2n = 20, CHN. Russia, Far East, Primorskii Krai, Khassanskii Raion, Reid Pallady, Mramornaya Bay, marine terrace, 17 Jun 2014, V.Yu. Barkalov 12642 (VLA). ASTERACEAE Ambrosia artemisiifolia L. 2n = 36, CHN. Russia, Far East, Primorskii Krai, Khankaiskii Raion, Platono-Aleksandrovskoe village, 23 Sep 2015, V.A. Nechaev 12949 (VLA). Cirsium vlassovianum Fisch. ex DC. 2n = 28, CHN. Russia, East Siberia, Zabaikal’skii Krai, Nerchinsko-Zavodskii Raion, near Ivanovka village, 665 m, forb meadow, 28 Aug 2013, S.G. Kazanovsky 12524 (IRK, VLA). Grindelia squarrosa (Pursh) Dunal 2n = 12, CHN. Russia, Far East, Primorskii Krai, Khankaiskii Raion, Platono-Aleksandrovskoe village, V.A. Nechaev 12885 (VLA). * Hieracium korshinskyi Zahn 2n = 36, CHN. Russia, East Siberia, Republic of Buryatia, Kabanskii Raion, Khamar-Daban mountain ridge, the Vydrinaya River, near the mouth, 492 m, the edge of the birch grassy-forb forest, 17 Jul 2015, S.G. Kazanovsky 12950 (IRK, VLA). Ixeridium gramineum (Fisch.) Tzvelev 2n = 14, CHN. Russia, East Siberia, Republic of Buryatia, outskirts of Ulan-Ude city, Yuzhnyi settlement, 544 m, in the light pine forest with steppe vegetation, 16 Jul 2015, S.G. Kazanovsky 12897 (IRK, VLA). Kalimeris incisa (Fisch.) DC. 2n = 18, CHN. Russia, Far East, Primorskii Krai, Khassanskii Raion, the Reid Pallady Bay, near Mramornyi Cape, sandy coast, at the edge of the grass bog, 7 Jun 2014, V.Yu. Barkalov 12633 (VLA). Senecio viscosus L. 2n = 40, CHN. Russia, East Siberia, Republic of Buryatia, Kabanskii Raion, the Baikal Lake, SE lakeside, the railway station Mishikha, along the forest road close to the railroad embankment, 4 Oct 2009, M.M. Ivanova 12927 (IRK, VLA). Tephroseris kirilowii (Turcz. ex DC.) Holub 2n = 48, CHN. Russia, Far East, Primorskii Krai, Khankaiskii Raion, 5 km of Platonovka village, moist meadow, 21 May 2015, V.A. Nechaev 12764 (VLA). BRASSICACEAE Brassica elongata subsp. integrifolia (Boiss.) Breistr. 2n = 30, CHN. Russia, East Siberia, Irkutskaya Oblast’, Ust’Udinskii Raion, in vicinity of Igzhey village, fallow land, 28 Aug 2008, A.V. Verkhozina, S.G. Kazanovsky & O.A. Chernyshova 12898 (IRK, VLA). Camelina microcarpa Andrz. ex DC. 2n = 16, CHN. Russia, East Siberia, Republic of Buryatia, Kabanskii Raion, near Nyuki village, the Selenga River, left riverside, 467 m, grassy-forb steppe, 17 Jul 2015, S.G. Kazanovsky 12934 (IRK, VLA). Lepidium apetalum Willd. 2n = 16, CHN. Russia, East Siberia, Republic of Buryatia, outskirts of Ulan-Ude city, Yuzhnyi settlement, 544 m, in the light pine forest with steppe vegetation, 18 Jul 2015, S.G. Kazanovsky 12930 (IRK, VLA). Version of Record E13 Marhold & Kučera (eds.) • IAPT/IOPB chromosome data 22 TAXON 65 (5) • October 2016: E1–E27 CAMPANULACEAE Campanula patula L. 2n = 20, CHN. Russia, East Siberia, Republic of Buryatia, Kabanskii Raion, near the railway station Vydrino, 557 m, roadside of the highway, 14 Jul 2014, S.G. Kazanovsky 12914 (IRK, VLA). CAPRIFOLIACEAE Patrinia rupestris (Pall.) Dufr. 2n = 22, CHN. Russia, Far East, Primorskii Krai, Ussuriyskii Raion, near Aleksei-Nikol’skoe village, V.A. Nechaev 12891 (VLA). CARYOPHYLLACEAE Dianthus versicolor Fisch. ex Link 2n = 30, CHN. Russia, Far East, Primorskii Krai, Khassanskii Raion, the Reid Pallady Bay, Mramornaya Mt., meadow on the slope, 16 Jun 2014, V.Yu. Barkalov 12618 (VLA). Lychnis flos-cuculi L. 2n = 24, CHN. Russia, East Siberia, Irkutskaya Oblast’, Slyudyanskii Raion, the Baikal Lake, SE lakeside, between Bol’shie Mangyly and Malye Mangyly rivers, 5377-th km, picket 5 of the East Siberian railroad, meadow close to the railway embankment, 9 Aug 2013, M.M. Ivanova 12932 (IRK, VLA). ** Spergularia rubra (L.) J.Presl & C.Presl 2n = 18, CHN. Russia, East Siberia, Republic of Buryatia, Kabanskii Raion, between the railway station Vydrino and Tolbazikha village, fine rubbly slope along the highway, 4 Jul 2014, M.M. Ivanova 12908 (IRK, VLA). CHENOPODIACEAE Teloxys aristata (L.) Moq. 2n = 18, CHN. Russia, East Siberia, Irkutskaya Oblast’, Slyudyanskii Raion, near the railway station Kultuk, 155-th km, picket 2 of the Krugobaikal’skaya railroad, on steppe slope, 4 Sep 2014, M.M. Ivanova 12928 (IRK, VLA). ELAEAGNACEAE Hippophae rhamnoides L. 2n = 24, CHN. Russia, East Siberia, Republic of Buryatia, Kyakhtinskii Raion, near Ust’-Kyakhta settlement, 632 m, sandy grassy-forb steppe, 29 Jul 2014, S.G. Kazanovsky 12910 (IRK, VLA). ERICACEAE Rhododendron aureum Georgi 2n = 52, CHN. Russia, East Siberia, Irkutskaya Oblast’, Kazachinsko-Lenskii Raion, the Baikal’skii mountain ridge, 5 km E of the railway station Kunerma, 527 m, the Siberian pine and fir forest with green mosses and Pinus pumila, 10 Aug 2014, S.G. Kazanovsky 12915 (IRK, VLA). Vaccinium myrtillus L. 2n = 24, CHN. Russia, East Siberia, Republic of Buryatia, Kabanskii Raion, near Tolbazikha village, the Tolbazikha River, left riverside, 566 m, the Siberian pine, fir and birch forest with spruce, bilberries and forb, 15 Jul 2014, S.G. Kazanovsky 12920 (IRK, VLA). EUPHORBIACEAE Euphorbia lucorum Rupr. 2n = 28, CHN. Russia, Far East, Primorskii Krai, Khassanskii Raion, the Reid Pallady Bay, Mramornaya Mt., forb meadow on the slope, 16 Jun 2014, V.Yu. Barkalov 12620 (VLA). Euphorbia pseudochamaesyce Fisch. & C.A.Mey. 2n = 22, CHN. Russia, East Siberia, Republic of Bur yatia, Kyakhtinskii Raion, near Ust’-Kyran settlement, the Chikoi River, E14 left riverside, 587 m, sandy slope, forb steppe, 16 Aug 2015, S.G. Kazanovsky 12933 (IRK, VLA); Russia, East Siberia, Republic of Buryatia, Selenginskii Raion, 8 km S of the Selenga River, right riverside, 541 m, rocks at the road, on rubbly soil among stones, 17 Aug 2015, S.G. Kazanovsky 12931 (IRK, VLA). FABACEAE Glycyrrhiza pallidiflora Maxim. 2n = 16, CHN. Russia, Far East, Primorskii Krai, Khorol’skii Raion, near Sivakovka village, W lakeside of the Khanka Lake, 11 Mar 2016, V.A. Nechaev 12924 (VLA). Gueldenstaedtia verna (Georgi) Boriss. 2n = 16, CHN. Russia, Far East, Primorskii Krai, Ussuriyskii Raion, near Aleksey-Nikol’skoe, V.A. Nechaev 12869 (VLA). Podocarpium mandshuricum (Maxim.) Czerep. 2n = 22, CHN. Russia, Far East, Primorskii Krai, MuravevAmurskii Peninsula, Vladivostok, oak forest near Akademgorodok, 5 Aug 2015, V.A. Nechaev 12810 (VLA). GENTIANACEAE * Swertia baicalensis Popov ex Pissjauk. 2n = 32, CHN. Russia, East Siberia, Irkutskaya Oblast’, Slyudyanskii Raion, the Snezhnaya River basin, in vicinity of the Pik Tal’tsinskii Mt., the upper course of the Tal’tsy River, 1093 m, subalpine forb meadow, 10 Aug 2015, S.G. Kazanovsky 12916 (IRK, VLA). LAMIACEAE Dracocephalum ruyschiana L. 2n = 14, CHN. Russia, East Siberia, Irkutskaya Oblast’, Irkutskii Raion, in vicinity of Bol’shoe Goloustnoe settlement, Zavirukha River - left tributary of the Goloustnaya River, 492 m, the pine and birch grassy-forb forest, 21 Jul 2014, S.G. Kazanovsky 12911 (IRK, VLA). Leonurus macranthus Maxim. 2n = 20, CHN. Russia, Far East, Primorskii Krai, Ussuriyskii Raion, near Nikolo-L’vovskoe settlement, 2015, V.A. Nechaev 12892 (VLA). LILIACEAE Lilium buschianum Lodd. 2n = 24, CHN. Russia, Far East, Primorskii Krai, Khassanskii Raion, the Reid Pallady Bay, Mramornaya Mt., meadow on the slope, 16 Jun 2014, V.Yu. Barkalov 12625 (VLA). PLANTAGINACEAE ** Veronica krylovii Schischk. 2n = 18, CHN. Russia, East Siberia, Irkutskaya Oblast’, Irkutskii Raion, in vicinity of Bol’shoe Goloustnoe settlement, Zavirukha River – left tributary of the Goloustnaya River, 486 m, grassy-forb meadow, 22 Jul 2014, S.G. Kazanovsky 12905 (IRK, VLA). POACEAE Achnatherum sibiricum (L.) Keng ex Tzvelev 2n = 24, CHN. Russia, West Siberia, Republic of Altai, Ongudaiskii Raion, near Ongudai settlement, 958 m, stony S slope with steppe vegetation, 30 Aug 2012, S.G. Kazanovsky 12561 (IRK, VLA). Agrostis clavata Trin. 2n = 42, CHN. Russia, Far East, Primorskii Krai, Khassanskii Raion, the Reid Pallady Bay, near Mramornyi Cape, sandy shore, 17 Jun 2014, V.Yu. Barkalov 12648 (VLA). Version of Record Marhold & Kučera (eds.) • IAPT/IOPB chromosome data 22 TAXON 65 (5) • October 2016: E1–E27 Calamagrostis angustifolia Kom. 2n = 28, CHN. Russia, Far East, Primorskii Krai, Khankaiskii Raion, in vicinity of Komissarovo settlement, the valley of Komissarovka River, moist depression, 26 Sep 2014, V.A. Nechaev 12695 (VLA). Viola extremiorientalis Vorosch. & N.S.Pavlova 2n = 24, CHN. Russia, Far East, Primorskii Krai, Nadezhdinskii Raion, upper course of the Nezhinka River, Ploskii Ridge, at the rocks of the watershed, 23 May 2014, V.Yu. Barkalov 12587 (VLA). Poa angustifolia L. 2n = 56, CHN. Russia, Far East, Primorskii Krai, Nadezhdinskii Raion, near Tavrichanka settlement, the shore of Amurskii Bay, meadow, 5 Jun 2015, V.A. Nechaev 12781 (VLA). Viola muehldorfii Kiss 2n = 24, CHN. Russia, Far East, Primorskii Krai, Nadezhdinskii Raion, the Razdol’naya River basin, the watershed of the Vtoraya Rechka River and Razdol’nenskii Spring (the confluent of Nezhinka River), mixed forest, 21 May 2014, V.Yu. Barkalov 12588 (VLA). Spodiopogon sibiricus Trin. 2n = 40, CHN. Russia, Far East, Primorskii Krai, Khassanskii Raion, near the lake Ptichye, Priozernaya Mt., stony slope with shrubs, 17 Jun 2014, V.Yu. Barkalov 12652 (VLA). POLYGONACEAE Rheum rhabarbarum L. 2n = 44, CHN. Russia, East Siberia, Irkutskaya Oblast’, Ol’khonskii Raion, the Baikal Lake, Aya Bay, 483 m, N slope, sandy steppe with Koeleria and Poa, 16 Aug 2014, S.G. Kazanovsky 12907 (IRK, VLA). Viola primorskajensis (W.Becker) Vorosch. 2n = 24, CHN. Russia, Far East, Primorskii Krai, Nadezhdinskii Raion, upper course of the Nezhinka River, near the mouth of its confluent Razdol’nensky Spring, at the riverside rocks, on the melkozem, 22 May 2014, V.Yu. Barkalov 12590 (VLA). * Viola woroschilovii Bezd. 2n = 24, CHN. Russia, Far East, Primorskii Krai, Vladivostok city, near Medical Institute, on the W slope to Ostryakova Ave., 15 Jun 2015, T.A. Bezdeleva 12904 (VLA). RANUNCULACEAE Aquilegia sibirica Lam. 2n = 16, CHN. Russia, East Siberia, Irkutskaya Oblast’, Slyudyanskii Raion, left riverside of the Snezhnaya River, 563 m, the Siberian pine and fir forest, on the butt of reversed Siberian pine, 13 Jul 2014, S.G. Kazanovsky & M.M. Ivanova 12917 (IRK, VLA). ROSACEAE Fragaria viridis Weston 2n = 14, CHN. Russia, East Siberia, Republic of Buryatia, Kabanskii Raion, near Nyuki village, the Selenga River, left riverside, 467 m, the pine forest with shrubs and forb, 17 Jul 2015, S.G. Kazanovsky 12938 (IRK, VLA). Spiraea alpina Pall. 2n = 18, CHN. Russia, East Siberia, Irkutskaya Oblast’, Kazachinsko-Lenskii Raion, the Kharakhikta River, 631 m, riverside shrubs, 11 Aug 2014, S.G. Kazanovsky 12900 (IRK, VLA). RUBIACEAE Galium vaillantii DC. 2n = 22, CHN. Russia, East Siberia, Irkutskaya Oblast’, Slyudyanskii Raion, the Baikal Lake, SW lakeside, the Krugobaikal’skaya railroad, 102-d km, the Shumikha River, at the steps to the river, 11 Aug 2014, M.M. Ivanova 12929 (IRK, VLA). SOLANACEAE Hyoscyamus niger L. 2n = 34, CHN. Russia, East Siberia, Zabaikal’skii Krai, Aginskii Raion, 10 km NW of Yuzhnyi Argaley village, the Argaley River, 896 m, at the road, 28 Aug 2013, S.G. Kazanovsky & E.A. Bondarevich 12906 (IRK, VLA); Russia, Far East, Primorskii Krai, Khassanskii Raion, near Andreevka settlement, coastal sands, among Leymus mollis, 15 Jun 2014, V.A. Nechaev 12586 (VLA). VIOLACEAE Viola brachysepala Maxim. 2n = 20, CHN. Russia, Far East, Primorskii Krai, Khassanskii Raion, the Reid Pallady Bay, Mramornaya Mt., stony slope, 16 Jun 2014, V.Yu. Barkalov 12645 (VLA). Viola collina Besser 2n = 20, CHN. Russia, Far East, Primorskii Krai, Nadezhdinskii Raion, upper course of the Nezhinka River, near the mouth of its confluent Razdol’nensky Spring, 24 May 2014, V.Yu. Barkalov 12583 (VLA). Nina S. Probatova,1* Denis A. Krivenko2 & Alexandr L. Ebel3 1 Institute of Biology & Soil Science, Far East Branch of the Russian Academy of Sciences, 159 Stoletya Ave., 690022 Vladivostok, Russia 2 Siberian Institute of Plant Physiology & Biochemistry, Siberian Branch of the Russian Academy of Sciences, 132 Lermontova Str., 664033 Irkutsk, Russia 3 National Research Tomsk State University, 36 Lenina Ave., 634050 Tomsk, Russia * Author for correspondence: probatova@ibss.dvo.ru * First chromosome count for the species. ** New chromosome number (cytotype) for the species. ALLIACEAE Allium vodopjanovae N.Friesen 2n = 16, CHN. Russia, East Siberia, Republic of Khakassia, Shirinskii Raion, near Efremkino settlement, right riverside of the Belyi Iyus River, rocky massif Togyz-Az, on rocks, 15 Aug 2013, A.L. Ebel 12750 (TK). AMARANTHACEAE Amaranthus retroflexus L. 2n = 32, CHN. Russia, East Siberia, Irkutskaya Oblast’, Ust’Ordynskii Buryatskii natsional’nyi okrug, Ekhirit-Bulagatskii Rayon, 51 km of Kachug tract, 9 km SE from the Zady village, forbs-grass steppe meadow, 16 Aug 2011, D.A. Krivenko 12601 (IRK, VLA). Atriplex hortensis L. 2n = 18, CHN. Russia, East Siberia, Irkutskaya Oblast’, Irkutsk city, the territory of the health resort “Angara”, 458 m, ruderal community at the roadside, 27 Jul 2014, D.A. Krivenko 12848 (IRK, VLA). Corispermum ulopterum Fenzl ex Ledeb. 2n = 18, CHN. Russia, East Siberia, Republic of Buryatia, Severo-Baikal’skii Raion, near Nizhneangarsk town, the Baikal Lake, Yarki Island, 521 m, on shore sands, 8 Aug 2014, D.A. Krivenko 12865 (IRK, VLA). ASPARAGACEAE Asparagus schoberioides Kunth 2n = 20, CHN. Russia, Far East, Primorskii Krai, Khassanskii Version of Record E15 Marhold & Kučera (eds.) • IAPT/IOPB chromosome data 22 TAXON 65 (5) • October 2016: E1–E27 Raion, Gamova Cape, on the road from the Vityaz’ Bay to the Astafieva Bay, 37 m, oak tall grasses forest, 19 Oct 2013, D.A. Krivenko 12605 (IRK, VLA). Trifolium lupinaster L. 2n = 32, CHN. Russia, East Siberia, Republic of Khakassia, Shirinskii Raion, near Efremkino, light Larix forest, 16 Aug 2013, A.L. Ebel 12774 (TK). ASTERACEAE Gnaphalium sylvaticum L. 2n = 56, CHN. Russia, East Siberia, Republic of Buryatia, Severo-Baikal’skii Raion, near the railway station Davan, 955 m, on pebbles of the riverside, 9 Aug 2015, D.A. Krivenko 12857 (IRK, VLA). Hypericum maculatum Crantz 2n = 16, CHN. Russia, West Siberia, Tomskaya Oblast’, in vicinity of Tomsk city, dry meadow near the ash dump of the electric power station - 2, 23 Aug 2013, A.L. Ebel 12753 (TK). Hieracium umbellatum agg. 2n = 27, CHN. Russia, East Siberia, Irkutskaya Oblast’, Kazachinsko-Lenskii Raion, 15 km of Okunaiskii settlement, the Kholodnoe Lake, 425 m, on the site of dry saline pond, in small pool, 11 Aug 2014, D.A. Krivenko 12867 (IRK, VLA). ** Iris ensata Thunb. 2n = 24+0–1B, CHN. Russia, Far East, Primorskii Krai, Nadezhdinskii Raion, in vicinity of the railway station Razdol’noe, D.A. Krivenko 12945 (IRK, VLA). Jacobaea erucifolia (L.) G.Gaertn., B.Mey. & Scherb. 2n = 40, CHN. Russia, East Siberia, Republic of Sakha-Yakutia, Mirninskii Rayon, right bank of the Ulakhan-Botuobuya River, about 4.7 km NNE of the mouth Mata-Ulegir River, 300 m, riverside, 2 Sep 2012, A.V. Verkhozina & D.A. Krivenko 12566 (IRK, VLA). Saussurea salicifolia DC. 2n = 26, CHN. Russia, East Siberia, Irkutskaya Oblast’, Ol’khonskiiRaion, the lakeside of the Baikal Lake, Aya Bay, 455 m, stony steppe, 16 Aug 2014, D.A. Krivenko 12834 (IRK, VLA). BORAGINACEAE Hackelia deflexa Opiz 2n = 24, CHN. Russia, East Siberia, Republic of Khakassia, Shirinskii Raion, in vicinity of Efremkino settlement, lower part of the slope to the valley of Belyi Iyus River, among shrubs, 17 Aug 2013, A.L. Ebel 12736 (TK). HYPERICACEAE IRIDACEAE LAMIACEAE Galeopsis bifida Boenn. 2n = 32, CHN. Russia, East Siberia, Irkutskaya Oblast’, Irkutskii Raion, the locality Mel’nichnaya Padj’, country place “Kedr”, wayside stop, 535 m, ruderal community, 23 Aug 2014, E.D. Krivenko & D.A. Krivenko 12854 (IRK, VLA). MALVACEAE Hibiscus trionum L. 2n = 56, CHN. Russia, Far East, Primorskii Krai, Nadezhdinskii Raion, in vicinity of Taezhnoe settlement (far from Kiparissovo railway station), as a weed on the garden-plot, 2015, E.G. Rudyka 12921 (VLA). ONAGRACEAE * Epilobium pseudorubescens A.K.Skvortsov 2n = 36, CHN. Russia, West Siberia, Tomskaya Oblast’, Tomsk city, on the city dump, 23 Aug 2013, A.L. Ebel 12734 (TK). BRASSICACEAE Capsella bursa-pastoris (L.) Medik. 2n = 16, CHN. Kazakhstan, Yuzhno-Kazakhstanskaya Oblast’, Kentau city, waste ground, 24 Apr 2013, A.N. Kupriyanov & A.L. Ebel 12724 (TK). Erucastrum armoracioides Litv. 2n = 30, CHN. Kazakhstan, Karagandinskaya Oblast’, Ossakarovskii Raion, 50 km northwards of Karaganda city, near Mirnoe settlement, by the roadside, 22 Apr 2013, A.L. Ebel 12609 (TK). EUPHORBIACEAE Euphorbia virgata Waldst. & Kit. 2n = 60, CHN. Russia, East Siberia, Republic of Buryatia, Pribaikal’skii Raion, near Turuntaevo village, left riverside of the Itantsa River, the grass community with ruderal weeds at the roadside, 6 Jul 2015, D.A. Krivenko 12849 (IRK, VLA). PAPAVERACEAE Papaver nudicaule L. 2n = 42, CHN. Russia, East Siberia, Irkutskaya Oblast’, Ol’khonskii Raion, the Baikal Lake, Ol’khon Island, Khuzhirskii Bay, 767 m, overdeveloped sandy-stony steppe, 15 Aug 2014, D.A. Krivenko 12853 (IRK, VLA). PLANTAGINACEAE Cymbalaria muralis G.Gaertn., B.Mey. & Scherb. 2n = 14, CHN. Russia, Far East, Primorskii Krai, suburbs of Vladivostok city, the Botanical Garden-Institute FEB RAS, as a weed on garden-beds, 2015, T.A. Bezdeleva 12902 (VLA). Plantago asiatica L. 2n = 24, CHN. Russia, Far East, Primorskii Krai, W lakeside of the Khanka Lake in vicinity of Turii Rog settlement, a lakelet in 300 m of the Khanka Lake, 26 Jul 2003, V.P. Verkholat & al. 9047 (VLA). FABACEAE Hedysarum turczaninovii Peschkova 2n = 14, CHN. Russia, East Siberia, Republic of Khakassia, Shirinskii Raion, near Efremkino, right riverside of the Belyi Iyus River, rocky massif Togyz-Az, stony steppe, 9 Aug 2013, A.L. Ebel 12775 (TK). Echinochloa colona (L.) Link 2n = 54, CHN. China, Henan Province, Loyan city, near Magnolia Hotel, ruderal community, 10 Dec 2014, D.A. Krivenko 12833 (IRK, VLA). ** Oxytropis nuda Basil. 2n = 16 + 0–4B, CHN. Russia, East Siberia, Republic of Khakassia, Shirinskii Raion, near Efremkino, right riverside of the Belyi Iyus River, rocky massif Togyz-Az, stony steppe, 9 Aug 2013, A.L. Ebel 12752 (TK). Melica turczaninowiana Ohwi 2n = 18, CHN. Russia, East Siberia, Irkutskaya Oblast’, Nizhneudinskii Raion, Ukovskii waterfall, near Vodopadnyi settlement, the Bol’shoi Uk River (left tributary of the Uda River), rocky slope, 28 Jul 2015, D.A. Krivenko 12835 (IRK, VLA). E16 POACEAE Version of Record Marhold & Kučera (eds.) • IAPT/IOPB chromosome data 22 TAXON 65 (5) • October 2016: E1–E27 Panicum miliaceum L. 2n = 36, CHN. Russia, East Siberia, Irkutskaya Oblast’, Ussol’skii Raion, Ussol’e-Sibirskoe town, park near the Culture Center “Khimik”, 442 m, the overgrown flower-bed, 23 Aug 2008, A.V. Verkhozina 12039 (IRK, VLA). Stipa krylovii Roshev. 2n = 44, CHN. Russia, East Siberia, Irkutskaya Oblast’, Ol’khonskii Raion, the lakeside of the Baikal Lake, Aya Bay, 455 m, stony steppe, 16 Aug 2014, D.A. Krivenko 12840 (IRK, VLA). POLYGONACEAE Aconogonon ochreatum Nakai 2n = 20, CHN. Russia, East Siberia, Republic of Buryatia, Severo-Baikal’skii Raion, near Nizhneangarsk town, the Baikal Lake, Yarki Island, 521 m, on shore sands, 8 Aug 2014, D.A. Krivenko 12851 (IRK, VLA). Rumex gmelinii Turcz. 2n = 40, CHN. Russia, East Siberia, Irkutskaya Oblast’, Slyudyanskii Raion, right riverside of the Kultuchnaya River, near the wayside stop Verbnyi, steppe meadow, 7 Jul 2015, D.A. Krivenko 12839 (IRK, VLA). PRIMULACEAE Androsace filiformis Retz. 2n = 20, CHN. Russia, Far East, Primorskii Krai, Mikhailovskii Raion, near Novoshakhtinskii settlement, 2015, V.T. Lapenko 12925 (VLA). RANUNCULACEAE Ranunculus sceleratus L. 2n = 32, CHN. Russia, East Siberia, Irkutskaya Oblast’, Irkutskii Raion, 4 km of the road to Mel’nichnaya Pad’, country place “Stroitel”, a weed on the dacha plot, 29 Jun 2015, E.V. Pradedova 12863 (IRK, VLA). 2n = 48, CHN. Russia, East Siberia, Irkutskaya Oblast’, Taishetskii Raion, near Shelaevo settlement, the Solonechnoe Lake, left riverside of the Biryussa River, birch forest with tall herbs, 26 Jul 2015, D.A. Krivenko 12862 (IRK, VLA). Viola sacchalinensis H.Boissieu 2n = 20, CHN. Russia, East Siberia, Republic of Buryatia, Selenginskii Raion, near Yagodnoe village, 711 m, along the riverside, 30 Jun 2015, D.A. Krivenko 12858 (IRK, VLA). Nina S. Probatova,1* Vitaly P. Seledets2 & Elvira G. Rudyka1 1 Institute of Biology & Soil Science, Far East Branch of the Russian Academy of Sciences, 159 Stoletya Prospect, 690022 Vladivostok, Russia 2 Pacific Institute of Geography, Far East Branch of the Russian Academy of Sciences, 7 Radio Street, 690041 Vladivostok, Russia * Author for correspondence: probatova@ibss.dvo.ru * First chromosome count for the species. ** New chromosome number (cytotype) for the species. ▲ CN counts were made by A.P. Sokolovskaya (unpub.). ACORACEAE Acorus calamus L. 2n = 24, CHN. Russia, Far East, Primorskii Krai, Khankaiskii Raion, W lakeside of the Khanka Lake, in vicinity of Il’inka settlement, 26 Jul 2003, V.P. Verkholat & al. 9046 (VLA). ADOXACEAE Adoxa moschatellina L. 2n = 36, CHN. Russia, Far East, Primorskii Krai, Shkotovskii Raion, near Shtykovo settlement, W slope of a hill, oak forest, 24 May 2006, A.E. Kozhevnikov & Z.V. Kozhevnikova 10161 (VLA). APIACEAE SCROPHULARIACEAE Scrophularia incisa Weinm. 2n = 48, CHN. Russia, East Siberia, Irkutskaya Oblast’, Ol’khonskii Raion, the lakeside of the Baikal Lake, Aya Bay, 455 m, sandy beach, 16 Aug 2014, D.A. Krivenko 12852 (IRK, VLA). * Scrophularia multicaulis Turcz. 2n = 24, CHN. Russia, East Siberia, Republic of Khakassia, Shirinskii Raion, near Efremkino, right riverside of the Belyi Iyus River, rocky massif Togyz-Az, stony slope, 17 Aug 2013, A.L. Ebel 12738 (TK). ** Cnidium dahuricum Fisch & C.A.Mey. 2n = 20, CHN. Russia, Far East, Primorskii Krai, Chernigovskii Raion, near Gornyi Khutor village, disturbed meadow, Jul 1995, Ya.I. Lelikov 7242 (VLA). ARACEAE Arisaema peninsulae Nakai 2n = 28, CHN. Russia, Far East, Primorskii Krai, Peschanyi Peninsula opposite Vladivostok city, valley forest, 3 Jun 2006, N.S. Pavlova 10201 (VLA). ASTERACEAE VIOLACEAE Viola hirta L. 2n = 20, CHN. Russia, East Siberia, Irkutskaya Oblast’, Taishetskii Raion, close to Shelaevo settlement, the Solonechnoe Lake, left riverside of the Biryussa River, birch forest with tall herbs, 26 Jul 2015, D.A. Krivenko 12860 (IRK, VLA). Viola philippica Cav. 2n = 48, CHN. China, Girin Province, Yan’byan’-Koreiskii Avtonomnyi Okrug, Khunchun’ city, in the city park, on the lawn, 10 Sep 2015, D.A. Krivenko 12936 (IRK, VLA). ** Viola ruppii All. 2n = 24, CHN. Russia, East Siberia, Irkutskaya Oblast’, Taishetskii Raion, close to Shelaevo settlement, the Solonechnoe Lake, left riverside of the Biryussa River, birch forest with tall herbs, 26 Jul 2015, D.A. Krivenko 12866 (IRK, VLA). Bidens tripartita L. 2n = 48, CHN. Russia, Far East, Primorskii Krai, Nadezhdinskii Raion, near the railway station Sovkhoznaya, coastal plain, 22 Sep 2007, V.A. Nechaev 10783 (VLA). Brachyactis angusta (Lindl.) Britt. 2n = 14, CHN. Russia, Far East, Primorskii Krai, Khankaiskii Raion, the railway station Kamen’-Rybolov, on the railroad embankment, 21 Sep 2011, V.T. Lapenko 11969 (VLA). Ixeridium gramineum (Fisch.) Tzvelev 2n = 14, CHN. China, Heilongjiang Province, Harbin city, as a weed in the park, 2 Jul 2000, N.S. Probatova & V.P. Seledets 8950 (VLA). Version of Record E17 Marhold & Kučera (eds.) • IAPT/IOPB chromosome data 22 TAXON 65 (5) • October 2016: E1–E27 ** Leontodon autumnalis L. 2n = 12 + 0–2–3B, CHN. Russia, Far East, Primorskii Krai, Mikhailovskii Raion, 2 km SW of Novoshakhtinskii settlement, along the forest road, 15 Oct 2015, V.T. Lapenko 12846 (VLA). Elymus woroschilovii Prob. 2n = 42, CHN. Russia, Far East, Primorskii Krai, Khassanskii Raion, in vicinity of Zarubino settlement, Troitza Bay, coastal steep, 15 Sep 2013, E.A. Chubar’ 12427 (VLA). Phalacroloma strigosum (Muhl. ex Willd.) Tzvelev 2n = 27, CHN. Russia, Far East, Primorskii Krai, Mikhailovskii Raion, SW outskirts of Novoshakhtinskii settlement, at the railway embankment, 6 Jul 2008, V.T. Lapenko 11126 (VLA). Elytrigia villosa (Drobow) Tzvelev (= Agropyron karawaewii P.A.Smirn.) 2n = 28, CHN. Russia, East Siberia, Republic of Sakha-Yakutia, central part, 30 km of Kyssyl-Syr village, Staryi Tukulan sands, 25 Jul 2015, I.A. Galanina 12872 (VLA). Pulicaria vulgaris Gaertn. 2n = 18, CHN. Russia, North Caucasus, Krasnodarskii Krai, Abinskii Raion, in vicinity of Erivanskaya settlement, forest edge, 5 Sep 2009, N.S. Probatova & V.P. Seledets 11602 (VLA). BRASSICACEAE Eremopyrum triticeum (Gaertn.) Nevski ▲ 2n = 14, CHN. Russia, North Caucasus, Republic of Daghestan, 20 km W of Makhachkala city, near the former station KumtorKala, opposite the Sary-Kum sands, the Artemisia semi-desert with Zygophyllum, 13 Jul 1979, N.S. Probatova & V.P. Seledets 5560 (VLA). CARYOPHYLLACEAE Leymus interior (Hultén) Tzvelev ▲ 2n = 28, CHN. Russia, East Siberia, Republic of SakhaYakutia, Bulunskii Raion, right riverside of the Lena River, near Kjussjur settlement, on the slope of ravine to the brook, abundant, 3 Aug 1973, N.S. Probatova & V.P. Seledets 3773 (VLA). LILIACEAE Phalaroides arundinacea (L.) Rauschert ▲ 2n = 28, CHN. Russia, Far East, Kamchatskii Krai, Kamchatka Peninsula, Ust’-Bol’sheretskii Raion, near Pauzhetka settlement, the valley of Pauzhetka River (the Ozernaya River basin), silt bank of warm rivulet, 16 Jul 1969, N.S. Probatova & E.G. Rudyka 1940 (VLA). Rorippa palustris (L.) Besser 2n = сa. 32, CHN. China, Heilongjiang Province, outskirts of Harbin city, sandy bank of the Sungari River, 2 Jul 2000, N.S. Probatova & V.P. Seledets 8153 (VLA). Minuartia laricina Mattf. 2n = 26, CHN. Russia, Far East, Amurskaya Oblast’, 6 km NW of Belogorsk, the Tom’ River, high fluvial terrace near the mouth of the former river-bed, SW of Tomskoe settlement, dry grassy steppe meadow, 24 Aug 2006, V.P. Verkholat 10386 (VLA). Lloydia serotina (L.) Salisb. ex Rchb. 2n = 24, CHN. Russia, Far East, Kamchatskii Krai, Kamchatka Peninsula, Kl’uchevskaya sopka Volcano, near Podkova, about 1080 m, heathy-great bilberry plots of tundra on the scoria fields, 22 Aug 2005, V.P. Verkholat 10224 (VLA). POACEAE Anisantha tectorum (L.) Nevski 2n = 14, CHN. Russia, Volga Region, Astrakhanskaya Oblast’, Narimanovskii Raion, right riverside of the Volga River, near Volzhskii settlement, overgrowing sand-dunes, 27 Aug 2009, N.S. Probatova & V.P. Seledets 11606 (VLA). Briza media L. ▲ 2n = 14, CHN. Russia, Leningradskaya Oblast’, Oranienbaumskii Raion, near Glyadino village, the former overgrown sand-pit, 26 Jun 1976, N.S. Probatova 4345 (VLA); Russia, Leningradskaya Oblast’, Oredezhskii Raion, 10 km of Oredezh settlement, near Beloe village, meadow on the lakeside of the Beloe Lake, 11 Jul 1976, N.S. Probatova 4369 (VLA). ▲ 2n = 28, CHN. Russia, Leningradskaya Oblast’, Gatchinskii Raion, near Vyra settlement, the riverside of the Oredezh River, meadow in depression, 8 Jul 1976, N.S. Probatova 4358 (VLA). Bromopsis australis (Zherebina) Tzvelev & Prob. ▲ 2n = 28, CHN. Kazakhstan, 20 km E of Kegen’ settlement, the Ili River basin, 2200 m, the slope to Karkara River, mountain steppe, 22 Jul 1978, N.S. Probatova & V.P. Seledets 5133 (VLA); Kyrgyzstan, N lakeside of the Issyk-Kul’ Lake, 20 km N of Semënovka settlement, the canyon Semenovskoe, ca. 2000 m, mountain slope, 30 Jul 1978, N.S. Probatova & V.P. Seledets 5175 (VLA). Digitaria ciliaris (Retz.) Koeler 2n = 36, CHN. Russia, North Caucasus, Krasnodarskii Krai, Abinskii Raion, outskirts of Abinsk town, as a weed in vegetable garden, 3 Sep 2009, N.S. Probatova & V.P. Seledets 12343 (VLA). E18 Poa angustifolia L. ▲ 2n = са. 70, CHN. Russia, Leningradskaya Oblast’, Gatchinskii Raion, outskirts of Vyra settlement, the riverside of the Oredezh River, meadow, 30 Jun 1974, A.P. Sokolovskaya 38 (VLA). Poa lapponica Prokudin 2n = 28, CHN. Russia, Republic of Karelia, Pitkyarantskii Raion, 20 km E of Sortavala town, the Ladozhskoe Lake, Kyryavalakhty Bay, Picea forest on the peninsula, 29 Sep 2008, N.S. Probatova & V.P. Seledets 11264 (VLA). Poa skvortzovii Prob. ▲ 2n = 42, CHN. Russia, Far East, Primorskii Krai, Terneiskii Raion, Sikhote-Alin’skii biosphere reserve, 18 km S of Ternei settlement, near Blagodatnoe Lake, forest brook, 10 Aug 1979, N.S. Probatova 5567 (VLA). Setaria maximowiczii Tzvelev & Prob. 2n = 18, CHN. Russia, Far East, Primorskii Krai, Partizanskii Raion, Vostok Bay, seacoast, 25 Sep 2002, V.A. Nechaev 8959 (VLA). POLYGONACEAE Persicaria amphibia (L.) S.F.Gray (terrestrial form) 2n = 66, CHN. Russia, Far East, Primorskii Krai, Khankaiskii Raion, W lakeside of the Khanka Lake, in vicinity of Turyi Rog settlement, 300 m of the lakeside of the Khanka Lake, 26 Jul 2003, V.P. Verkholat & al. 9051 (VLA). Rumex acetosa L. 2n = 14, CHN. Russia, Far East, Primorskii Krai, Khankaiskii Raion, W lakeside of the Khanka Lake, in vicinity of Turyi Rog settlement, 26 Jul 2003, V.P. Verkholat & al. 9053 (VLA). Version of Record Marhold & Kučera (eds.) • IAPT/IOPB chromosome data 22 TAXON 65 (5) • October 2016: E1–E27 RANUNCULACEAE * Aconitum woroschilovii Luferov (A. maximum auct.: Sokolovskaya, 1963) ▲ 2n = 32, CHN. Russia, Far East, Kamchatskii Krai, Kamchatka Peninsula, Bystrinskii Raion, near Esso settlement, right riverside of the Uksichan River, forb-sedge meadow, 30 Jul 1959, A.P. Sokolovskaya 201 (VLA). ** Caltha silvestris Worosch. 2n = 64, CHN. Russia, Far East, Primorskii Krai, Mikhailovskii Raion, 1.5 km SW of Novoshakhtinskii town, at the rivulet, 13 Oct 2015, V.T. Lapenko 12845 (VLA). ROSACEAE Potentilla paradoxa Nutt. ex Torr. & A.Gray 2n = 28, CHN. Russia, Far East, Primorskii Krai, MuravëvAmurskii Peninsula, Ussuriyskii Gulf, Emar Bay, marine terrace at the recreation centre “Yunga”, as a weed near the building, 27 Aug 2000, N.S. Probatova & V.P. Seledets 8754 (VLA); China, Heilongjiang Province, Suifen’khe city, as a weed in the street, 2 Jul 2000, N.S. Probatova & V.P. Seledets 8966 (VLA). Julia V. Shner,1 Tatiana V. Alexeeva,1 Abdullo Kurbonov2 & Michael G. Pimenov1* 1 Botanical Garden, Moscow State University, 119234 Moscow, Russia 2 Botany Department, Tajik State Pedagogical University, Rudaki Av. 121, 733740 Dushanbe, Republic of Tajikistan * Author for correspondence: mgpimenov@mail.ru * First chromosome count for the species. This study was supported by the grant 13-04-00648 from the Russian Foundation for Basic Research (RFBR) APIACEAE Aegopodium tadshikorum Schischk. n = 11, CHN. Tajikistan, Sughd prov., Kurama ridge, PangazBolo, 40°49.779′ N, 70°16.220′ E, 1810 m, 20 May 2015, M.G. Pimenov & A. Kurbonov 13 (MW) [Fig. 6A]. Chromosome numbers n = 11 or 2n = 22 were reported from Kazakhstan, Kyrgyzstan and Uzbekistan (Retina & Pimenov, 1977; Rostovtzeva, 1982; Vasil’eva & al., 1994; Shner & al., 2014). Material from Tajikistan was investigated here for the first time. Aphanopleura capillifolia (Regel & Schmalh.) Lipsky 2n = 22, CHN. Tajikistan, Sughd prov., Mogoltau ridge, vicinity of Uchbog and Aktash villages, 40°14.704′ N, 69°25.920′ E, 392 m, 29 May 2014, A. Kurbonov 349 (MW) [Fig. 7A]. Our chromosome number report is the fifth for the species (the fourth for native plants and the second for Tajik material) (Pimenov & al., 2002); all determinations show the species is diploid with x = 11. Berula erecta (Huds.) Coville n = 10, CHN. Tajikistan, Sughd prov., village of Sultanabad, 40°18.697′ N, 69°49.869′ E, 380 m, 28 May 2015, M.G. Pimenov & A. Kurbonov 48 (MW) [Fig. 6B]. More than 20 determinations of chromosome numbers for the species have been published, based on materials collected in Europe, Asia and North America (Pimenov & al., 2002). The cytotype with 2n = 18 is the most usual; B-chromosomes were reported once (Lövkvist & Hultgård, 1999). Other counts are rare: n = 6 was reported one time from the U.S.A. (Bell & Constance, 1957), 2n = 24 also one time from Slovakia (Kochjarová, 1992) whereas the cytotype with 2n = 20 was found in Sweden (Lövkvist, 1963) and Kazakhstan (Retina & Pimenov, 1977). Material from Tajikistan is investigated here for the first time; it corresponds to our previous report from Kazakhstan. Carum carvi L. 2n = 20, CHN. Tajikistan, Sughd prov., Kurama ridge, left bank of Pangaz river, environs of village of Khoito, marsh, 40°44.229′ N, 70°15.461′ E, 1292 m, 3 Jul 2013, A. Kurbonov 178 (MW); Tajikistan, Sughd prov., Kurama ridge, eastern slope of Bobosetan Mts, low mountains of Sheramardi, left bank of Pangaz river, 40°49.176′ N, 70°16.098′ E, 1888 m, 16 Jul 2013, A. Kurbonov 246 (MW) [Fig. 7F]. There are more than 50 chromosome number reports for C. carvi from Europe, Asia and North America. The cytotype 2n = 20 is the most widely distributed, whereas the cytotype 2n = 22 was found in Europe as well as in Asia (Pimenov & al., 2002). Material from Tajikistan is investigated here for the first time. Elaeosticta hirtula (Regel & Schmalh.) Kljuykov, Pimenov & V.N.Tikhom. n = 10, CHN. Tajikistan, Sughd prov., Kurama ridge, valley of Ashtcai river, above Obi-Asht, 40°52.167′ N, 70°26.860′ E, 23 May 2015, M.G. Pimenov & A. Kurbonov 36 (MW) [Fig. 6K]. The studied gathering belongs to the diploid cytotype, widely distributed in Middle Asia. Tetraploid cytotype (2n = 40) is more rare, being found in Tajikistan and Kyrgyzstan (Kljuykov, 1978). Eremodaucus lehmannii Bunge n = 9, CHN. Tajikistan, Sughd prov., valley of Sirdarya river, southern slope of Akchop low ridge, fixed sands, vegetation with dominance of Haloxylon, 40°18.976′ N, 69°55.347′ E, 424 m, 28 May 2015, M.G. Pimenov & A. Kurbonov 49 (MW) [Fig. 6D]. Material from Tajikistan is investigated here for the first time. Previously the counts of n = 9 and 2n = 18 for E. lehmannii were determined for plants from Uzbekistan and Turkmenistan (Vasil’eva & al., 1991; Alexeeva & al., 1994). Ferula karatavica Regel & Schmalh. 2n = 22, CHN. Tajikistan, Sughd prov., Mogoltau ridge, Spa Mt., Kaltasai gorge, 40°16.172′ N, 69°27.691′ E, 520 m, 30 May 2014, A. Kurbonov 375 (MW) [Fig. 7D]. Material from Tajikistan is investigated here for the first time. Chromosome count of 2n = 22 was previously determined from Kyrgyzstan (Retina & Pimenov, 1977; Solov’eva & al., 1982). * Ferula karelinii Bunge n = 11, CHN. Tajikistan, Sughd prov., valley of Sirdarya river, southern slope of Akchop low ridge, fixed sands, desert vegetation with dominance of Haloxylon, 40°18.976′ N, 69°55.347′ E, 420 m, 28 May 2015, M.G. Pimenov & A. Kurbonov 52 (MW) [Fig. 6G]. This is the first chromosome number report for the species, which sometimes is regarded as a member of an independent monotypic genus Schumannia Kuntze (S. turkomanica Kuntze = S. karelinii (Bunge) Korovin). The fruit structure of F. karelinii is, however, very similar to that of some species of true Ferula, belonging to Korovin’s subg. Merwia, especially to F. litwinowiana Koso-Pol. (Pimenov & Kirillina, 1980). Unfortunately, the chromosome number determination of F. karelinii is of low informative value, as, on one hand, n = 11 was found in all up to now studied species of Ferula (without any exception), but, on the other, this is the number, known for more than a half of karyologically investigated Umbelliferae species of different taxonomical position within the family. Ferula lithophila Pimenov n = 11, CHN. Tajikistan, Sughd prov., Kurama ridge, valley of fl. Dakhana sai, Dzhangalak gorge, near village of Dakhana, Version of Record E19 Marhold & Kučera (eds.) • IAPT/IOPB chromosome data 22 TAXON 65 (5) • October 2016: E1–E27 Fig. 6. Meiotic chromosomes. A, Aegopodium tadshikorum, n = 11; B, Berula erecta, n = 10; C, Prangos fedtschenkoi, n = 11; D, Eremodaucus lehmannii, n = 9; E, Ferula lithophila, n = 11; F, Oedibasis apiculata, n = 10; G, Ferula karelinii, n = 11; H, Schrenkia vaginata, n = 11; I, Prangos pabularia, n = 11; J, Pimpinella puberula, n = 10; K, Elaeosticta hirtula, n = 10; L, Ferula samarkandica, n = 11; M, Schrenkia golickeana, n = 11; N, Ferula penninervis, n = 11; O, Torilis arvensis, n = 6. ― Scale bars = 5 μm; n – nucleolus. E20 Version of Record Marhold & Kučera (eds.) • IAPT/IOPB chromosome data 22 TAXON 65 (5) • October 2016: E1–E27 Fig. 7. Mitotic chromosomes. A, Aphanopleura capillifolia, 2n = 22; B, Galagania tenuisecta, 2n = 22; C, Ferula penninervis, 2n = 22; D, Ferula karatavica, 2n = 22; E, Ferula mogoltavica, 2n = 22; F, Carum carvi, 2n = 20; G, Heracleum lehmannianum, 2n = 22; H, Sium medium, 2n = 12; I, Torilis leptophylla, 2n = 12; J, Torilis arvensis, 2n = 12; K, Scandix stellata, 2n = 20; L, Turgenia latifolia, 2n = 18. ― Scale bars = 5 μm. Version of Record E21 Marhold & Kučera (eds.) • IAPT/IOPB chromosome data 22 TAXON 65 (5) • October 2016: E1–E27 granit deluvium, 40°42.770′ N, 70°10.278′ E, 1446 m, 24 May 2015, M.G. Pimenov & A. Kurbonov 40 (MW) [Fig. 6E]. Material from Tajikistan is investigated here for the first time. Chromosome count of 2n = 22 was previously determined from Uzbekistan (Vasilieva & al., 1991). Ferula mogoltavica Lipsky ex Korovin 2n = 22, CHN. Tajikistan, Sughd prov., Mogoltau ridge, environs of Khudzhand, 40°19.093′ N, 69°36.341′ E, 707 m, 19 May 2014, A. Kurbonov 296 (MW) [Fig. 7E]. This is the second chromosome number report for this endemic species. Material for previous report (Retina & Pimenov, 1977) was collected approximately near the present locality. Ferula penninervis Regel & Schmalh. n = 11, CHN. Tajikistan, Sughd prov., Kurama ridge, village of Pangaz-bolo, 40°47.645′ N, 70°14.660′ E, 1450 m, 20 May 2015, M.G. Pimenov & A. Kurbonov 22 (MW) [Fig. 6N]. 2n = 22, CHN. Tajikistan, Sughd prov., Kurama ridge, right bank of Pangaz river, environs of Susor gorge, stony slope, 40°46.305′ N, 70°15.037′ E, 1500 m, 7 Jul 2013, A. Kurbonov 186 (MW) [Fig. 7C]. Material from Tajikistan is investigated here for the first time. Chromosome number of 2n = 22 was determined previously for the plants from Kazakhstan and Kyrgyzstan (Retina & Pimenov, 1977; Solov’eva & al., 1982). Ferula samarkandica Korovin n = 11, CHN. Tajikistan, Sughd prov., Kuroma ridge, above of Pangaz-bolo, 40°49.000′ N, 70°15.683′ E, 1700 m, 20 May 2015, M.G. Pimenov & A. Kurbonov 9 (MW) [Fig. 6L]. This is the second chromosome number report for this species and the first one from Tajikistan; it corresponds to the previous one from Uzbekistan (Vasil’eva & Pimenov, 1985). Galagania tenuisecta (Regel & Schmalh.) M.G.Vassiljeva & Pimenov 2n = 22, CHN. Tajikistan, Sughd prov., Mogoltau ridge, southern slope, between villages of Uchbog and Aktash, 40°16.172′ N, 69°27.691′ E, 520 m, 29 May 2014, A. Kurbonov 365 (MW) [Fig. 7B]. This is the fourth chromosome number report for this species, confirming three previous ones (Vasilieva & al., 1981; Vasilieva & al., 1991; Pimenov & Vasil’eva, 1983) for the plants from Kazakhstan and Tajikistan. Heracleum lehmannianum Bunge 2n = 22, CHN. Tajikistan, Sughd prov., Kurama ridge, left bank of Pangaz river, Gaznoi gorge, 40°44.280′ N, 70°15.483′ E, 1300 m, 26 Jun 2013, A. Kurbonov 171 (MW) [Fig. 7G]. We confirm here previous chromosome number reports for H. lehmannianum (2n = 22), made for plants from Tajikistan (Retina & Pimenov, 1977; Agapova, 1990), Uzbekistan (Shner & al., 2012), Afghanistan (Shner & al., 2015) and for cultivated material (Rostovtzeva, 1979). Oedibasis apiculata (Kar. & Kir.) Koso-Pol. n = 10, CHN. Tajikistan, Sughd prov., Mogoltau ridge, gorge of Devona-bolo, 40°19.362′ N, 69°36.195′ E, 619 m, 29 May 2015, M.G. Pimenov & A. Kurbonov 62 (MW) [Fig. 6F]. This is the second chromosome number report for this species and the first one from Tajikistan; the previous one (2n = 20) was published from Kyrgyzstan (Vasil’eva & al., 1981). Pimpinella puberula (DC.) Boiss. n = 10, CHN. Tajikistan, Sughd prov., between Uchbog and Aktash, 40°14.704′ N, 69°25.919′ E, 392 m, 31 May 2015, M.G. Pimenov & A. Kurbonov 70 (MW) [Fig. 6J]. E22 This species, as some other Pimpinella species, has two cytotypes – 2n = 18 (Turkmenistan) and 2n = 20 (Turkmenistan, Iran) (Vasil’eva & al., 1981; Yurtzeva, 1988; Shner & al., 2004). Material from Tajikistan is investigated here for the first time. Prangos fedtschenkoi (Regel & Schmalh.) Korovin n = 11, CHN. Tajikistan, Sughd prov., valley of Sirdarya River, low mountains of Ak-bel’, 40°25.204′ N, 70°08.867′ E, 638 m, 28 May 2015, M.G. Pimenov & A. Kurbonov 53 (MW) [Fig. 6C]. This is the second report of chromosome number for this species, confirming the first one (2n = 22) for the plants, collected also in Tajikistan (Pimenov & Vasil’eva, 1983). Prangos pabularia Lindl. n = 11, CHN. Tajikistan, Sughd prov., Kurama ridge, near Pangazbolo, 40°47.645′ N, 70°14.660′ E, 1530 m, 20 May 2015, M.G. Pimenov & A. Kurbonov 5 (MW) [Fig. 6I]. There is some incongruence in cytological data concerning P. pabularia. The first report (2n = 36) for plants from Afghanistan (Podlech & Dieterle, 1969) was not confirmed in following investigations of plants from adjacent regions (Kyrgyzstan, Tajikistan), which showed 2n = 22 (Vasil’eva & al., 1981, 1993; Pimenov & al. 1998; Shner & al., 2015), typical for the most species of the genus. Report of 2n = 22 from Turkey (Herrnstadt & Heyn, 1977), where P. pabularia s.str. does not occur, should be referred to P. lophoptera Boiss. Scandix stellata Banks & Sol. 2n = 20, CHN. Tajikistan, Sughd prov., Kurama ridge, upper reaches of the Pangaz river, Sheramardi Mts., breakstone slope, 40°47.071′ N, 70°14.800′ E, 1562 m, 8 Jul 2013, A. Kurbonov 187 (MW); Tajikistan, Sughd prov., Kurama ridge, the right bank of Obi-Asht, environs of village of Asht-bolo, Khayatabad gorge, 40°50.660′ N, 70°32.109′ E, 1200 m, 14 May 2013, A. Kurbonov 120 (MW) [Fig. 7K]. The chromosome numbers for this species were repeatedly reported for the plants from Southern Europe and various regions of Asia (Pimenov & al., 2002; Shner & al., 2014). Material from Tajikistan is investigated here for the first time, the number of 2n = 20 corresponds to the most of previous reports. Earlier the number of 2n = 22 was been determined by our team from Kazakhstan (Vasil’eva & al., 1991), 2n = 16, reported for plants under cultivation (Garde & Malheiros-Garde, 1949) refers, probably, to another species. Schrenkia golickeana (Regel & Schmalh.) B.Fedtsch. n = 11, CHN. Tajikistan, Sughd prov., Kurama ridge, near village of Pangaz-bolo, 40°48.776′ N, 70°15.281′ E, 1650–1710 m, 20 May 2015, M.G. Pimenov & A. Kurbonov 8, 10 (MW) [Fig. 6M]. This is the second chromosome number report for this species and the first one from Tajikistan; the previous one (2n = 22) was published from Kazakhstan (Vasil’eva & al., 1991). Schrenkia vaginata (Ledeb.) Fisch. & C.A.Mey. n = 11, CHN. Tajikistan, Sughd prov., Kurama ridge, valley of Ashtcai river, above Obi-Asht, 40°52.167′ N, 70°26.860′ E, 23 May 2015, M.G. Pimenov & A. Kurbonov 35 (MW) [Fig. 6H]. This is the fourth report on chromosome number of S. vaginata. The previous determinations were made for plants from Kyrgyzstan and Tajikistan (Vasil’eva & al., 1984, 1991); karyotype morphometric analysis showed karyotype being rather symmetrical (6m + 16sm). Sium medium Fisch. & C.A.Mey. 2n = 12, CHN. Tajikistan, Sughd prov., Kurama ridge, left bank of the Pangaz river, near gorges Gaznoi and Imodi, 40°44.280′ N, 70°15.483′ E, 1302 m, 16 Aug 2014, A. Kurbonov 397 (MW) [Fig. 7H]. Material from Tajikistan is investigated here for the first time. Earlier the same chromosome number was reported for the plants Version of Record Marhold & Kučera (eds.) • IAPT/IOPB chromosome data 22 TAXON 65 (5) • October 2016: E1–E27 from India, Pakistan and Kyrgyzstan (Pimenov & al., 2002; Shner & al., 2012). Torilis arvensis (Huds.) Link n = 6, CHN. Tajikistan, Sughd prov., Mogoltau Mts., western part, southern slope near Uchteppa, 40°14.117′ N, 69°27.266′ E, 31 May 2015, M.G. Pimenov & A. Kurbonov 69 (MW) [Fig. 6O]. 2n = 12, CHN. Tajikistan, Sughd prov., Mogoltau Mts., Karatoi Mt., Karatau gorge, 40°15.750′ N, 69°21.481′ E, 462 m, 29 May 2014, A. Kurbonov 334 (MW) [Fig. 7J]. The numerous (at least 30) reports on chromosome numbers of this species are invariant – the results were always n = 6 or 2n = 12 (Pimenov & al., 2002; Shner & al., 2004). Torilis leptophylla (L.) Rchb.f. 2n = 12, CHN. Tajikistan, Sughd prov., Mogoltau Mts., western part, southern slope, between villages of Uch-bog and Aktash, 40°14.704′ N, 69°25.919′ E, 392 m, 29 May 2014, A. Kurbonov 341 (MW) [Fig. 7I]. Chromosome numbers of T. leptophylla were reported for the plants from Greece, Spain, Morocco, various countries of Asia (Pimenov & al., 2002; Shner & al., 2004). The majority of determinations gave 2n = 12 (n = 6). In Spain the cytotype with n = 12 was also found (Silvestre, 1978, 1986). Material from Tajikistan is investigated here for the first time. Turgenia latifolia (L.) Hoffm. 2n = 18, CHN. Tajikistan, Sughd prov., Kurama ridge, BoboiOb Mts., Kukhsarsai, environs of village of Pangaz, 40°48.534′ N, 70°14.268′ E, 1800 m, 13 Aug 2013, A. Kurbonov 267 (MW) [Fig. 7L]. Chromosome numbers of T. latifolia were determined more than 10 times for the plants from Europe and Asia; these reports demonstrate moderate variability (2n = 18, 24, 32), with prevalence of the last of these numbers in recent reports (Pimenov & al., 2002). Tajik plants, studied previously, also have 2n = 32 (Vasil’eva & al., 1991). The cytotype with n = 9 (2n = 18) known from Iraq, Israel, Jordan and Pakistan (Constance & al., 1976; Cauwet-Marc & Jury, 1978; Al-Eisawi, 1989; Khatoon & Ali, 1993), is now found in Tajikistan. Literature cited Agapova, N.D. 1990. Apiaceae. Pp. 49–94 in: Takhtajan, A.L. (ed.), Numeri Chromosomatum Magnoliophytorum Florae URSS, vol. 1, Aceraceae – Menyanthaceae. Leninopoli: Nauka. Al-Eisawi, D.M.H. 1989. Chromosome counts of Umbelliferae of Jordan. Ann. Bot. (Rome) 47: 201–214. Alexeeva, T.V., Vasil’eva, M.G., Daushkevich, J.V. & Pimenov, M.G. 1994. [Reports] in: Stace, C.A. (ed.), IOPB chromosome data 8. Newslett. Int. Organ. Pl. Biosyst. 23: 11–12. Bell, C.R. & Constance, L. 1957. Chromosome numbers in Umbelliferae. Amer. J. Bot. 44: 565–572. http://dx.doi.org/10.2307/2438928 Cauwet-Marc, A.-M. & Jury, S. 1978. Données caryologiques sur la tribu des Caucalidées (Umbelliferae). Pp. 305–323 in: Cauwet-Marc, A.-M. & Carbonier, J. (eds.), Actes du 2e symposium international sur les Ombellifères, Perpignan, 1977, Contributions pluridisciplinaires à la systématique. Paris. Constance, L., Chuang, T.I. & Bell, R.A. 1976. Chromosome numbers in Umbelliferae. V. Amer. J. Bot. 63: 608–625. http://dx.doi.org/10.2307/2441824 Gardé, A. & Malheiros-Gardé, N. 1949. Contribuição para o estudo cariológico da familia Umbelliferae. I. Agron. Lusit. 11: 91–140. Herrnstadt, J. & Heyn, C.C. 1977. A monographic study of the genus Prangos (Umbelliferae). Boissiera 26: 1–91. Khatoon, S. & Ali, S.I. 1993. Chromosome numbers of some plants of Pakistan. Pakistan J. Bot. 14: 117–129. Kljuykov, E.V. 1978. Chisla khromosom nekotorykh vidov roda Elaeosticta Fenzl. Nauchnye Dokl. Vysshei Shkoly Biol. Nauki 9(177): 82–85. Kochjarová, J. 1992. Karyological study of the Slovak flora XXVIII. Acta Fac. Rerum Nat. Univ. Comen., Bot. 39: 67–74. Lövkvist, B. 1963. Berula erecta. P. 700 in: Weimarck, H. (ed.), Skanes flora. Lund: Bokförlaget Corona AB. Lövkvist, B. & Hultgård, U.M. 1999. Chromosome numbers in south Swedish vascular plants. Opera Bot. 137: 1–42. Pimenov, M.G. & Kirillina, N.A. 1980. The carpology of Soranthus, Ladyginia, Eriosynaphe and Schumannia in connection with the problem of taxonomic limits of the genus Ferula (Apiaceae). Bot. Zhurn. (Moscow & Leningrad) 65: 1756–1766. Pimenov, M.G. & Vasil’eva, M.G. 1983. [Reports] in: Löve, Á. (ed.), IOPB chromosome number reports LXXXI. Taxon 32: 663–664. Pimenov, M.G., Alexeeva, T.V., Artem’eva G.M. & Kljuykov, E.V. 1998. [Reports] in: Stace, C.A. (ed.), IOPB chromosome data 13. Newslett. Int. Organ. Pl. Biosyst. 29: 23–24. Pimenov, M.G., Vasil’eva, M.G., Leonov, M.V. & Daushkevich, J.V. 2002. Karyotaxonomical analysis in Umbelliferae. Enfield, Plymouth: Science Publishers. Podlech, D. & Dieterle, A. 1969. Chromosomenstudien an afghanischen Pflanzen. II. Candollea 24: 185–243. Retina, T.A. & Pimenov, M.G. 1977. Chisla khromosom vidov semeistva Umbelliferae Srednei Azii. Nauchnye Dokl. Vysshei Shkoly Biol. Nauki 7(163): 90–95. Rostovtzeva, T.S. 1979. Chromosome numbers of some species from the family Apiaceae Lindl. II. Bot. Zhurn. (Moscow & Leningrad) 64: 227–230. Rostovtzeva, T.S. 1982. Chromosome numbers of some species from the family Apiaceae. III. Bot. Zhurn. (Moscow & Leningrad) 67: 206–209. Shner, J.V., Pimenov, M.G., Kljuykov, E.V., Alexeeva, T.V., Ghahremaninejad, F. & Mozaffarian, V. 2004. Chromosome numbers in the Iranian Umbelliferae. Chromosome Sci. 8: 1–9. Shner, J.V., Alexeeva, T.V., Pimenov, M.G., Kljuykov, E.V., Ukrainskaya, U.A. & Zakharova, E.A. 2012. [Reports] in: Marhold, K. (ed.), IAPT/ IOPB chromosome data 14. Taxon 61: 1345, E30–E31. Shner, J.V., Alexeeva, T.V., Pimenov, M.G., Kljuykov, E.V., Ukrainskaya, U.A. & Zakharova, E.A. 2014. [Reports] in: Marhold, K. (ed.), IAPT/ IOPB chromosome data 18. Taxon 63: 1393, E30–E33. Shner, J.V., Alexeeva, T.V., Pimenov, M.G., Kljuykov, E.V., Ukrainskaya, U.A. & Zakharova, E.A. 2015. [Reports] in: Marhold, K. (ed.), IAPT/ IOPB chromosome data 19. Taxon 64: 1073–1074, E15–E21. Silvestre, S. 1978. Contribution al estitudio cariologico de la familia Umbelliferae en la peninsula Iberica. II. Lagascalia 7: 163–172. Silvestre, S. 1986. In: Números cromosomicos para la Flora Española 435–455. Lagascalia 14: 273–281. Solov’eva, N.M., Vasil’eva M.G., Pimenov, M.G., Turkov, V.D. & Zhigareva, N.N. 1982. Karyosystematic study of the genus Ferula (Umbelliferae). Bot. Zhurn. (Moscow & Leningrad) 67: 1228–1237. Vasil’eva, M.G., Pimenov, M.G., Solov’eva, N.M. & Kljuykov, E.V. 1981. Chisla khromosom vidov semeistva Umbelliferae Srednei Azii. II. Nauchnye Dokl. Vysshei Shkoly Biol. Nauki 12(216): 58–62. Vasil’eva, M.G., Solov’eva, N.M., Pimenov, M.G. & Vaulina, E.L. 1984. Kariotipy nekotorykh endemichnykh taxonov Umbelliferae Srednei Azii. Nauchnye. Dokl. Vysshei Shkoly Biol. Nauki 3(243): 66–74. Vasil’eva, M.G., Alexeeva, T.V., Pimenov, M.G. & Kljuykov, E.V. 1991. [Reports] in: Stace, C.A. (ed.), IOPB chromosome data 3. Newslett. Int. Organ. Pl. Biosyst. 17: 10–13. Vasil’eva, M.G., Daushkevich, J.V., Alexeeva, T.V. & Pimenov, M.G. 1993. [Reports] in: Stace, C.A. (ed.), IOPB chromosome data 5. Newslett. Int. Organ. Pl. Biosyst. 20: 7–9. Vasil’eva, M.G., Alexeeva, T.V. & Pimenov, M.G. 1994. Geographical variation of chromosome numbers in thin-rhizomatous species of Aegopodium (Umbelliferae). Bot. Zhurn. (Moscow & Leningrad) 79: 27–30. Vasil’eva, M.G. & Pimenov, M.G. 1985. [Reports] in: Löve, Á. (ed.), IOPB chromosome number reports LXXXVIII. Taxon 34: 547–548. Yurtzeva, O.V. 1988. Tsitologicheskoe izuchenie nekotorykh vidov roda Pimpinella (Umbelliferae-Apioideae). Nauchnye Dokl. Vysshei Shkoly Biol. Nauki 11(301): 78–85. Version of Record E23 Marhold & Kučera (eds.) • IAPT/IOPB chromosome data 22 TAXON 65 (5) • October 2016: E1–E27 Régine Verlaque,1* Annie Aboucaya,2 Virgile Noble,3,4 Laurent Hardion5 & Bruno Vila1 1 Institut Méditerranéen de Biodiversité et d’Ecologie (IMBE, UMR CNRS 7263), Aix-Marseille Univ., 3 Place V. Hugo, Case 4, 13331 Marseille Cedex 3, France 2 Parc National de Port-Cros, Allée du Castel Sainte-Claire, BP 70220, 83406 Hyères Cedex, France 3 Conservatoire Botanique National Méditerranéen de Porquerolles, 34 Avenue Gambetta, 83400 Hyères, France 4 Institut Méditerranéen de Biodiversité et d’Ecologie (IMBE, UMR CNRS 7263), Aix-Marseille Univ., Europôle Méditerranéen de l’Arbois, 13100 Aix-en-Provence, France 5 Université de Strasbourg. Laboratoire Image, Ville, Environnement (LIVE, UMR 7362 CNRS). Institut de Botanique, 28 rue Goethe, 67083 Strasbourg Cedex, France * Author for correspondence: regine.verlaque@imbe.fr * First chromosome count for the taxon. ▼ First chromosome count from French populations. AMARANTHACEAE * Corispermum gallicum Iljin 2n = 18, CHN. France, Var, Vinon-sur-Verdon, sandy banks, 28 Oct 2003, CBNMED-Noble S13-04 (MARS) [Fig. 8A]. This is the first count for this annual endemic species of SE France (0–400 m). The same diploid number has been found in all other Corispermum species studied. APIACEAE Bupleurum praealtum L. 2n = 16, CHN. France, Alpes-Maritimes, Vésubie Valley, Levens, cliff, 450 m, 17 Jun 2009, Verlaque & Vila F09-146 (MARS 10319). * Heracleum pumilum Vill. 2n = 22, n = 11, CHN. France, Vaucluse, Mt. Ventoux, Combe de Fonfiole, scree at ca. 1900 m, 6 Jul 1979, Verlaque F79-739 (MARS 14001) [Fig. 8B]. This is the first count for this endemic species of SW Alps (1400– 2600 m). Despite its atypical morphology, this slender species possesses the same diploid number as most of Heracleum species studied. APOCYNACEAE ▼ Nerium oleander L. 2n = 22, CHN. France, Var, Fréjus, Estérel Massif, wadi with Nerium oleander, Myrtus and Arundo donaciformis, 7 May 2011, Hardion S11-140 (MARS); Greece, near Missolonghi, alluvial deposits of Evinos River, reed-bed, 10 Apr 2011, Hardion S11-139 (MARS). Owing to its ornamental use, this monotypic shrubby genus has been poorly studied. These first counts in situ from France and Greece agree with the only previous report from Sicilian natural populations (Larsen & Lagaard, 1971). ▼ Hyoseris taurina (Pamp.) Martinoli 2n = 16, CHN. France, Var, Hyères, Giens, siliceous littoral, rock crevices, 19 Mar 2011, CBNMED-Noble S11-118 (MARS). E24 BORAGINACEAE ▼ Buglossoides incrassata subsp. permixta (Jord.) R.Fern. 2n = 28, CHN. France, Var, Ampus, Barjaude Mountain, 30 May 2010, CBNMED-Noble S14-03 (MARS). New for France, our count of this SW European taxon confirms previous reports from Spain (Luque & Diaz Lifante, 1991). Therefore, this is the lowest ploidy level in the poorly known B. incrassata clade (Cecchi & al., 2014) since the related B. incrassata (Guss.) I.M.Johnst. subsp. incrassata possesses 2n = 42 (Luque & Valdés, 1984, under the name B. arvensis subsp. gasparrinii (Heldr.) R.Fernandes). The second widespread B. arvensis clade is characterized by the series: 2n = 28, 36, 42, 56 (e.g., Strid & Franzen, 1981). CAMPANULACEAE All materials fixed on flowers (F) or seedlings (S) produced from seeds collected by us or by the Conservatoire Botanique National Méditerranéen (CBNMED), and counted by R. Verlaque and B. Vila; vouchers in MARS. This study was supported by the Parc National de Port-Cros and the CBNMED. The nomenclature follows recent French flora treatments (Tison & Foucault, 2014; Tison & al., 2014); only new counts are drawn. The authors are grateful to colleagues who helped us with plant location and sampling. ASTERACEAE This first count from France confirms previous reports (Martinoli, 1953; Peruzzi & Vangelisti, 2010) for this endemic taxon of SW Sardinia, recently found in France. Other Mediterranean related taxa possess the same chromosome number. * Solenopsis corsica (Meikle) M.B.Crespo, Serra & Juan 2n = 28, CHN. France, C Corsica, Bocagnano, Cascade du Voile de la Mariée, pond, 800 m, 6 Jul 1966, Contandriopoulos F66-8 (MARS 10332) [Fig. 8C]. This is the first count for this perennial endemic species located in Corsican and N Sardinian wetlands (100–1400 m). The same tetraploid number characterizes two other perennial Mediterranean endemic taxa: S. bivonae (Tineo) Crespo & al. from S Sardinia and Sicilia (Romano & al., 1986; Peruzzi & Gargano, 2003, as S. minuta subsp. nobilis (Winner) Meikle) and S. minima (Sims) Crespo & al. from Mallorca (Castro & Rossello, 2005, as S. minuta subsp. balearica (Winner) Meikle). * Solenopsis laurentia (L.) C.Presl 2n = 14, CHN. France, Var, Massif des Maures, Vallée de la Molle, Val d’Enfer, wetland, 5 Jun 2013, Aboucaya F13-11 (MARS), Aboucaya F13-12 (MARS), Aboucaya F13-13 (MARS 10317); France, Var, Port-Cros Island, Vallon de la Solitude, temporary pond, 5 Jun 2013, Aboucaya F13-10 (MARS 10316) [Fig. 8D]. Our counts are the first for this protected species scattered in Mediterranean wetlands. This diploid number is very important to understand the differentiation of this difficult genus (Crespo & al., 1998). The diploid annual species differ from other tetraploid perennial and insular endemic taxa by their erect stem with cauline leaves (vs. leaves in basal rosette, without stem) and their greater corolla (5–7 vs. 3–5 mm). With 2n = 22 (Aldridge & Ortega, 1976), only the annual endemic S. canariensis C.Presl from Tenerife is an exception. CARYOPHYLLACEAE * Bufonia perennis Pourr. (s.str.) 2n = 16, CHN. France, Var, Estérel, rocky dry meadow, 18 Jun 2008, Aboucaya F08-12 (MARS 14002). 2n = 16 + 2B, CHN. France, Var, Estérel, dry rocky meadow, 18 Jun 2008, Aboucaya F08-11 (MARS 08094) [Fig. 8E]. Our counts for this perennial endemic species from S France highlight the first W Mediterranean diploid of this poorly studied genus. The same number has been only found in B. parviflora Gris. from Crimea and Armenia (Nussbaumer, 1964). The presence of B-chromosomes in our plants could be related to the counts 2n = 18 in Greek endemics: B. stricta (Sm.) Gurke (Montmollin, 1984) and B. euboica Phitos & Kamari (1992). In the Mediterranean area, all other perennial (B. macropetala Willk., B. mauritanica Murb.: 2n = 34) and annual taxa (B. tenuifolia L. 2n = 34, B. paniculata Delarbre 2n = 32) are tetraploid. The chromosome number of the closely related B. tuberculata Loscos (E Spain) is unknown. Version of Record Marhold & Kučera (eds.) • IAPT/IOPB chromosome data 22 TAXON 65 (5) • October 2016: E1–E27 CAPRIFOLIACEAE Knautia collina (Guérin) Jord. 2n = 20, CHN. France, Var, Vallon de la Foux, between Méounes and Belgentier, along a forest path, 20 May 2009, Verlaque F09-130 (MARS 10315). EUPHORBIACEAE ▼ Euphorbia davidii Subils. 2n = 56, CHN. France, Bouches-du-Rhône, Carry-le-Rouet, rocky place along the railway, 11 Jun 2010, Verlaque S10-04 (MARS 10331). Belonging to the N. American E. dentata complex, this high polyploid and annual taxon is now naturalized in France. FABACEAE ▼ Vicia dalmatica A.Kern. 2n = 12, CHN. France, Var, after Tourves, meadow near the roadside, 1 Jun 1990, Verlaque F90-17; France, Var, between Roquebrussanne and Tourves, clearing in a pine forest, 20 May 2009, Verlaque F09-127 (MARS 10318). This ruderal NE Mediterranean species is now naturalized in SE France. With the SW European V. incana Gouan, they seem to be the progenitors of the paleo-temperate allotetraploid V. tenuifolia. ▼Serapias parviflora Parl. 2n = 36, n = 18, CHN. France, Var, Hyères, Tombolo of Giens, Les Pesquiers, meadows behind the salt marsh, 18 May 2011, Aboucaya F11-211 (MARS), Aboucaya F11-212 (MARS 10333) For this Mediterranean-Atlantic species protected in France, our counts agree with previous reports from Italy (e.g., Mazzola & al., 1981) and Portugal (Queiros, 1983). * Serapias strictiflora Welw. ex Veiga 2n = 72, CHN. France, Var, Le Cannet-des-Maures, Escarcets Lake, siliceous wet meadow, 8 May 2010, Aboucaya F10-27 (MARS 08104), Aboucaya F10-26 (MARS 08103), Aboucaya F10-25 (MARS 08105) [Fig. 8G]. These are the first counts for the two latter taxa recently rehabilitated with a specific status. Compared to the closely related widespread S. lingua L. (Mediterranean-Atlantic, 0–1100 m), these rare taxa occur at low altitudes (0–200 m), on siliceous substrate, in a smaller area: SE France (Provence, Corsica) and W Mediterranean, respectively. Serapias lingua, S. olbia, S. strictiflora share the same polyploid level, dense clonal populations, and labellum morphology (with solitary black ridge at base vs. two divergent ridges at base in taxa with 2n = 36). Giving their chromosome numbers, hybrid origin Vicia tenuifolia Roth 2n = 24, CHN. France, Var, between Roquebrussanne and Tourves, clearing in a pine forest, 20 May 2009, Verlaque F09-128 (MARS 10324). LILIACEAE ▼ Fritillaria involucrata All. 2n = 24, CHN. France, Var, Maures Massif, La Molle, clearing, 30 m, 21 Apr 2011, Noble F11-114 (MARS 10323). This species has been poorly studied and generally from botanical garden plants (Darlington & La Cour, 1941; La Cour, 1951). From a southern isolated location, we confirm previous reports for this French endemic taxon of SW Alps. Lilium martagon L. 2n = 24, CHN. France, Bouches-du-Rhône, Ste-Baume Massif, forest, near 900 m, 20 May 2009, Verlaque F09-136 (MARS 14005). ▼ Tulipa agenensis DC. 2n = 24, CHN. France, Bouches-du-Rhône, Venelles, cereal field, 23 Mar 1995, Filosa F95-03 (MARS 14006). Early counts mainly concern botanical garden plants (Hall, 1937; Woods & Banford, 1937, under the name T. oculus-solis St-Amans). Like many ornamental Tulipa, this taxon is considered as introduced in Europe from C Asia. Curiously in France this species is only diploid, while in the E Mediterranean, in Greece (Athanasiou, 1988) and Israel (Horovitz & Galil, 1972), it is di- and triploid. ORCHIDACEAE ▼ Anacamptis coriophora subsp. fragrans (Pollini) R.M.Bateman, Pridgeon & M.W.Chase 2n = 38, CHN. France, Var, Gapeau Valley, between Signes and Méounes, Rosmarinus shrubland, siliceous rocky site, 500 m, 20 May 2009, Verlaque F09-120 (MARS 10312) For this Mediterranean species, protected in France, our count confirms the only previous report from Sardinian plants (Scrugli & al., 1976). * Serapias olbia Verg. 2n = 72, CHN. France, Var, Giens, Darboussière, backshore, maritime sand, 18 May 2011, Aboucaya F11-13 (MARS 10329) [Fig. 8F]. Fig. 8. A , Corispermum gallicum, 2n = 18; B, Heracleum pumilum, n = 11; C, Solenopsis corsica, 2n = 28; D, S. laurentia, 2n = 14; E, Bufonia perennis, 2n = 16 + 2B; F, Serapias olbia, 2n = 72; G, S. strictiflora, 2n = 72; H , Polygala nicaensis subsp. nicaensis, 2n = 34; I, Scrophularia canina subsp. pinnatifida var. deschatresii, 2n = 24; J, S. canina subsp. ramosissima, n = 12; K, S. provincialis, 2n = 24. ― Scale bar = 10 μm. Version of Record E25 Marhold & Kučera (eds.) • IAPT/IOPB chromosome data 22 TAXON 65 (5) • October 2016: E1–E27 hypotheses of S. strictiflora or S. olbia (S. lingua × S. parviflora: Moore, in Tutin & al., 1980) must be rejected. POACEAE ▼ Narduroides salzmanii (Boiss.) Rouy 2n = 14, CHN. France, Var, Signes, Gapeau Valley, maquis, sandy place, 20 May 2009, Verlaque F09-118 (MARS 10313) and Verlaque F09-123 (MARS 10314). This W Mediterranean rare and small species forms a monotypic annual genus. At its N limit, our new count from France agrees with the previous report from S Spain (Bailey & Stace, 1989). POLYGALACEAE * Polygala nicaeensis Risso ex W.D.J.Koch subsp. nicaeensis 2n = 34, CHN. France, Var, Estérel Massif, maquis, 18 Jun 2008, Aboucaya F08-13 (MARS 08093) [Fig. 8H]. Our first count of this Ligurian endemic taxon is in agreement with those found in the Corsican vicariant endemic P. nicaensis subsp. corsica (Boreau) Graeb. (Contandriopoulos, 1962). This aneuploid number (2n =34) is the more common within this difficult genus. SCROPHULARIACEAE * Scrophularia canina subsp. pinnatifida var. deschatresii Gamisans 2n = 24, n = 12; France, N Corsica, near Ponte Leccia, scree, 250 m, 12 May 1995, Verlaque F95-136 (MARS 10321) [Fig. 8I]. * Scrophularia canina subsp. ramosissima (Loisel.) P.Fourn. 2n = 24, n = 12, CHN. France, Var, Ramatuelle, beach of Pampelonne, 12 May 1994, Aboucaya F94-27 (MARS 14003); France, Corsica, Ajaccio, Campo del Oro, sand on the backshore, 9 May 1995, Verlaque S95-103 (MARS 10322) [Fig. 8J]. * Scrophularia provincialis Rouy 2n = 24, CHN. France, Alpes-Maritimes, Bréas Road, the slope of Mt-Vial, rocky site, 450 m, 17 Jun 2009, Verlaque & Vila F09-147 (MARS 08097); France, Alpes-Maritimes, near Bourdon, 750 m, 19 Apr 2011, CBNMED-Noble F11-115 (MARS 10330); France, Var, Bois de Clappes, clearing, 700 m, 5 Jun 2012, Verlaque F12-114 (MARS 10320) [Fig. 8K]. Our counts are the first for these three endemic taxa of SE France (Corsica, Tyrrhenian area, and Provence, respectively). In this genus, many high polyploid and aneuploid species (2n > 36) have been found, but the numbers 2n = 24, 26 mainly occur in the perennial S. sect. Canina Don, mostly distributed in the Middle-East (Ortega Olivencia & Devesa Alcaraz, 1990). In Europe, the very rare euploid number 24 characterizes some Mediterranean suffruticose endemics, such as S. crithmifolia Boiss. (S & E Spain) and the three studied taxa. In contrast, the rest of the S. canina group – more continental widespread, polymorphous and less robust – possesses 2n = 26 (30), with sometimes B chromosomes (e.g., Vaarama & Leikas, 1970; Ortega Olivencia & Devesa Alcaraz, 1990). These results confirm Richardson’s proposal (in Tutin & al., 1972) regarding S. canina var. minoricensis P. Monts. 2n = 26 (Cardona, 1977): “plants from Islas Balearic referred to S. canina subsp. ramosissima are in many features transitional to subsp. canina”. Verbascum boerhavii L. 2n = 44–45, CHN. France, Alpes-Maritimes, La trinité, Plateau Tercier, rocky site, 800 m, 11 May 2009, Verlaque & Vila F09-106 (MARS 10325). Like Natarajan (1977), we found the same odd chromosome number for this W Mediterranean species, while its closely related species V. thapsus L. (W & C Europe) possesses 2n = 36. E26 Verbascum sinuatum L. 2n = 30, CHN. France, Var, Les Pesquiers, pine-forest, 13 May 2009, Aboucaya F09-138 (MARS 10326). We confirm several previous reports, but not 2n = 18 found in S-C France by Labadie (1974). If SE France is a noteworthy pole of plant biodiversity, karyological research on many poorly known taxa is still needed. Of the 27 studied taxa, including a dozen French endemic taxa, we have found 11 new chromosome numbers and 10 first counts for France. Karyological traits exhibit biogeographical particularities in this flora: the 21 diploid taxa (or with a lower ploidy level) show its major role for conservation, while the 5 higher ploidy levels testify to its active differentiation. Literature cited Aldridge, A. & Ortega, J. 1976. Estudios en la Flora de Macaronesia: Algunas numeros de cromosomoas II. Bot. Macaronés, IV Ci. 2: 9–19. Athanasiou, K. 1988. Some cytogeographical notes on the genus Tulipa L. (Liliaceae). Bot. Chron. 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Version of Record Marhold & Kučera (eds.) • IAPT/IOPB chromosome data 22 TAXON 65 (5) • October 2016: E1–E27 Ortega Olivencia, A. & Devesa Alcaraz, J. A. 1990. Contribución al estudio cariológico del género Scrophularia L. (Scrophulariaceae) en la Península Ibérica. Lagascalia 16: 171–198. Peruzzi, L. & Gargano, D. 2003. Mediterranean chromosome number reports. Flora Medit. 13: 382–387. Peruzzi, L. & Vangelisti, R. 2010. Considerazioni tassonomiche su Hyoseris taurina (Asteraceae) e sua presenza in Italia centrale. Ann. Bot. (Rome) ser. 2, 2009 Suppl.: 119–134. Phitos, D. & Kamari, G. 1992. Bufonia euboica (Caryophyllaceae), a new species from Greece. Willdenowia 22: 81–83. Queiros, M. 1983. Numeos cromossomicos para la Flora Italiana: 238–249. Inform. Bot. Ital. 8: 82–91. Romano, S., Mazzola, P. & Raimondo, F. M. 1986. Numeri cromosomici per la flora Italiana: 1070–1081. Inform. Bot. Ital. 18: 159–167. Scrugli, A., Martis, B. de & Mulas, B. 1976. 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