Fungal diversity notes 367–490: taxonomic and phylogenetic contributions to fungal taxa

ANIL RAJ

Novel species of microfungi described in the present study include the following from South Africa: Camarosporium aloes, Phaeococcomyces aloes and Phoma aloes from Aloe, C. psoraleae, Diaporthe psoraleae and D. psoraleae-pinnatae from Psoralea, Colletotrichum euphorbiae from Euphorbia, Coniothyrium prosopidis and Peyronellaea prosopidis from Prosopis, Diaporthe cassines from Cassine, D. diospyricola from Diospyros, Diaporthe maytenicola from Maytenus, Harknessia proteae from Protea, Neofusicoccum ursorum and N. cryptoaustrale from Eucalyptus, Ochrocladosporium adansoniae from Adansonia, Pilidium pseudoconcavum from Greyia radlkoferi, Stagonospora pseudopaludosa from Phragmites and Toxicocladosporium ficiniae from Ficinia. Several species were also described from Thailand, namely: Chaetopsina pini and C. pinicola from Pinus spp., Myrmecridium thailandicum from reed litter, Passalora pseudotithoniae from Tithonia, Pallidocercospora ventilago from Ventilago, Pyricularia bothriochloae from Bothriochloa and Sphaerulina rhododendricola from Rhododendron. Novelties from Spain include Cladophialophora multiseptata, Knufia tsunedae and Pleuroascus rectipilus from soil and Cyphellophora catalaunica from river sediments. Species from the USA include Bipolaris drechsleri from Microstegium, Calonectria blephiliae from Blephilia, Kellermania macrospora (epitype) and K. pseudoyuccigena from Yucca. Three new species are described from Mexico, namely Neophaeosphaeria agaves and K. agaves from Agave and Phytophthora ipomoeae from Ipomoea. Other African species include Calonectria mossambicensis from Eucalyptus (Mozambique), Harzia cameroonensis from an unknown creeper (Cameroon), Mastigosporella anisophylleae from Anisophyllea (Zambia) and Teratosphaeria terminaliae from Terminalia (Zimbabwe). Species from Europe include Auxarthron longisporum from forest soil (Portugal), Discosia pseudoartocreas from Tilia (Austria), Paraconiothyrium polonense and P. lycopodinum from Lycopodium (Poland) and Stachybotrys oleronensis from Iris (France). Two species of Chrysosporium are described from Antartica, namely C. magnasporum and C. oceanitesii. Finally, Licea xanthospora is described from Australia, Hypochnicium huinayensis from Chile and Custingophora blanchettei from Uruguay. Novel genera of Ascomycetes include Neomycosphaerella from Pseudopentameris macrantha (South Africa), and Paramycosphaerella from Brachystegia sp. (Zimbabwe). Novel hyphomycete genera include Pseudocatenomycopsis from Rothmannia (Zambia), Neopseudocercospora from Terminalia (Zambia) and Neodeightoniella from Phragmites (South Africa), while Dimorphiopsis from Brachystegia (Zambia) represents a novel coelomycetous genus. Furthermore, Alanphillipsia is introduced as a new genus in the Botryosphaeriaceae with four species, A. aloes, A. aloeigena and A. aloetica from Aloe spp. and A. euphorbiae from Euphorbia sp. (South Africa). A new combination is also proposed for Brachysporium torulosum (Deightoniella black tip of banana) as Corynespora torulosa. Morphological and culture characteristics along with ITS DNA barcodes are provided for all taxa.

To assess behaviours and attitudes of residents toward giving news of death in emergency department and other departments. The study was conducted between 1st and 7th December, 2012, in an urban hospital in Ankara, Turkey. It used a questionnarie that was filled by 100 residents from different disciplines of medicine. Categorical variables were analysed with Chi-square and Fisher's exact tests and continuous variables were analysed with Mann Whitney test. The level of statistical significance was set at 0.05. The most difficult cases to notify were those of unexpected and sudden deaths (n = 51; 51%) followed by deaths of children (n = 36; 36%). While 60% (n = 60) of the study group reported a need for training in this area, but there was no association between having difficulty in notifying a death and expressing the need for training (p = 0.187). Residents who had difficulty in notifying death informed the patient's close ones more often during resuscitation (p = 0.049) and...

Personnel Information System development in organizations or institutions institutions currently very rapidly with the number of publications and test the application of some researchers at universities around the world, Problems of Information Systems always be obstacles and barriers in the development of every organization. Identify the problem that there can be improvements in the development of Personnel Information System. The system is expected to provide facilities and improve work effectiveness and can produce a fast and accurate information. The method used to analyze the system, data processing and system design, information system can cope with delays in the process of computer-based data input. can collect data - the data from the Personnel Employee and Payroll into a single entity in the database, so it can be processed and presented into a useful information for the company

Fungal Diversity (2016) 80:1–270 DOI 10.1007/s13225-016-0373-x Fungal diversity notes 367–490: taxonomic and phylogenetic contributions to fungal taxa Kevin D. Hyde1,2,3 • Sinang Hongsanan3,32 • Rajesh Jeewon55 • D. Jayarama Bhat12,13 • Eric H. C. McKenzie20 • E. B. Gareth Jones5 • Rungtiwa Phookamsak15 • Hiran A. Ariyawansa9 • Saranyaphat Boonmee3 • Qi Zhao1,2,28 • Faten Awad Abdel-Aziz6 • Mohamed A. Abdel-Wahab5,6 • Supharat Banmai3 • Putarak Chomnunti10 • Bao-Kai Cui38 • Dinushani A. Daranagama3,4 • Kanad Das26 • Monika C. Dayarathne2,3 • Nimali I. de Silva2,3,14,15 • Asha J. Dissanayake3,30 • Mingkwan Doilom3 • Anusha H. Ekanayaka2,3,15 • Tatiana Baptista Gibertoni56 • Aristóteles Góes-Neto50 • Shi-Ke Huang2,3 • Subashini C. Jayasiri3,29 • Ruvishika S. Jayawardena3,30 • Sirinapa Konta3 • Hyang Burm Lee39 • Wen-Jing Li3 • Chuan-Gen Lin3,11 • Jian-Kui Liu3,9 • Yong-Zhong Lu3,29 • Zong-Long Luo3,10,31 • Ishara S. Manawasinghe3,30 • Patinjareveettil Manimohan58 • Ausana Mapook3 • Tuula Niskanen49 • Chada Norphanphoun3 • Moslem Papizadeh51 • Rekhani H. Perera3,9 • Chayanard Phukhamsakda2,3 • Christian Richter17,18 • André L. C. M. de A. Santiago40 • E. Ricardo Drechsler-Santos42 • Indunil C. Senanayake2,3,15 • Kazuaki Tanaka7,8 • Received: 27 July 2016 / Accepted: 30 August 2016 / Published online: 28 September 2016 Ó School of Science 2016 Electronic supplementary material The online version of this article (doi:10.1007/s13225-016-0373-x) contains supplementary material, which is available to authorized users. & Qi Zhao zhaoqi@mail.kib.ac.cn Kevin D. Hyde kdhyde3@gmail.com 1 2 3 4 Institute of Biotechnology and Germplasm Resources; Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation Ministry of Agriculture; Yunnan Provincial Key Laboratory of Agricultural Biotechnology, Yunnan Academy of Agricultural Sciences, Kunming 650223, Yunnan, People’s Republic of China Key Laboratory of Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan, People’s Republic of China Center of Excellence in Fungal Research, Mae Fah Luang University, 333 M. 1, T. Thasud, Muang, Chiang Rai 57100, Thailand State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, No 3 1st West Beichen Road, Chaoyang District, Beijing 100101, People’s Republic of China 5 Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box: 2455, Riyadh, 1145, Saudi Arabia 6 Department of Botany and Microbiology, Faculty of Science, Sohag University, Sohag 82524, Egypt 7 Faculty of Agriculture and Life Sciences, Hirosaki University, 3 Bunkyo-cho, Hirosaki, Aomori 036-8561, Japan 8 The United Graduate School of Agricultural Sciences, Iwate University, 18-8 Ueda 3 Chome, Morioka 020-8550, Japan 9 Guizhou Guizhou Guiyang People’s 10 School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand 11 Department of Plant Pathology, College of Agriculture, Guizhou University, Guiyang 550025, Guizhou, People’s Republic of China 12 Department of Botany, Goa University, Taleigão, Goa, India 13 No. 128/1–J, Azad Housing Society, Curca, Goa Velha 403108, India Key Laboratory of Agricultural Biotechnology, Academy of Agricultural Sciences, Xiaohe District, City, Guizhou Province 550006, Republic of China 123 2 Fungal Diversity (2016) 80:1–270 T. M. D. S. Tennakoon2,3,15 • Kasun M. Thambugala3,9 • Qing Tian2,3,10,15,16 • Saowaluck Tibpromma3,15,32 • Benjarong Thongbai3 • Alfredo Vizzini35 • Dhanushka N. Wanasinghe2,3 • Nalin N. Wijayawardene3 • Hai-Xia Wu43 • Jing Yang3 • Xiang-Yu Zeng3,29 • Huang Zhang33 • Jin-Feng Zhang9 • Timur S. Bulgakov19 • Erio Camporesi21,22,23 • Ali H. Bahkali5 • Mohammad A. Amoozegar54 • Lidia Silva Araujo-Neta56 • Joseph F. Ammirati48 • Abhishek Baghela27 • R. P. Bhatt24 • Dimitar Bojantchev47 • Bart Buyck25 • Gladstone Alves da Silva56 • Catarina Letı́cia Ferreira de Lima40 • Rafael José Vilela de Oliveira40 • Carlos Alberto Fragoso de Souza40 • Yu-Cheng Dai38 • Bálint Dima45,46 • Tham Thi Duong39 • Enrico Ercole35 • Fernando Mafalda-Freire42 • Aniket Ghosh24 • Akira Hashimoto7,8 • Sutakorn Kamolhan3 • Ji-Chuan Kang29 • Samantha C. Karunarathna2,3,15,16,32 • Paul M. Kirk57 • Ilkka Kytövuori44 • Angela Lantieri36 • Kare Liimatainen45,49 • Zuo-Yi Liu9 • Xing-Zhong Liu4 • Robert Lücking34 • Gianfranco Medardi37 • Peter E. Mortimer2,15,16 • Thi Thuong Thuong Nguyen39 • Itthayakorn Promputtha14 • K. N. Anil Raj58 • Mateus A. Reck41 • Saisamorn Lumyong14 • Seyed Abolhassan Shahzadeh-Fazeli51,53 • Marc Stadler17,18 • Mohammad Reza Soudi52 • Hong-Yan Su31 • Takumasa Takahashi7 • Narumon Tangthirasunun3,59 • Priyanka Uniyal24 • Yong Wang11 • Ting-Chi Wen29 • Jian-Chu Xu2,15,16 • Zhong-Kai Zhang1 • Yong-Chang Zhao1 • Jun-Liang Zhou38 • Lin Zhu38 Abstract This is a continuity of a series of taxonomic papers where materials are examined, described and novel combinations are proposed where necessary to improve our traditional species concepts and provide updates on their classification. In addition to extensive morphological descriptions and appropriate asexual and sexual 14 Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand 25 15 World Agroforestry Centre, East and Central Asia, Kunming 650201, Yunnan, People’s Republic of China Muséum National d’Histoire Naturelle, Département Systématique et Evolution, CP 39, ISYEB, UMR 7205 CNRS MNHN UPMC EPHE, 12 Rue Buffon, 75005 Paris, France 26 Cryptogamic Unit, Botanical Survey of India, P.O. Botanic Garden, Howrah 711103, India 27 National Fungal Culture Collection of India (NFCCI), Biodiversity and Palaeobiology Group, MACS - Agharkar Research Institute, G.G. Agarkar Road, Pune 411004, India 28 Institute of Edible Fungi, Yunnan Agricultural University, Kunming 650201, Yunnan, People’s Republic of China 29 Engineering Research Center of Southwest BioPharmaceutical Resources, Ministry of Education, Guizhou University, Guiyang 550025, Guizhou Province, People’s Republic of China 30 Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, People’s Republic of China 31 College of Agriculture & Biological Sciences, Dali University, Dali 671003, Yunnan, People’s Republic of China 32 Mushroom Research Foundation, 128 M.3 Ban Pa Deng T. Pa Pae, A. Mae Taeng, Chiang Mai 50150, Thailand 33 Yunnan Institute of Food Safety, Kunming University of Science & Technology, Kunming 650500, Yunnan, People’s Republic of China 16 17 18 Centre of Mountain Ecosystem Studies, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan, People’s Republic of China Department of Microbial Drugs, Helmholtz Centre for Infection Research, Brunswick, Germany German Centre for Infection Research (DZIF), Partner Site Hannover/Braunschweig, Inhoffenstrasse 7, 38124 Brunswick, Germany 19 Academy of Biology and Biotechnology, Southern Federal University, Rostov-on-Don 344090, Rostov Region, Russia 20 Landcare Research Manaaki Whenua, Private Bag 92170, Auckland, New Zealand 21 Gruppo Micologico Forlivese ‘‘Antonio Cicognani’’, Via Roma 18, Forlı̀, Italy 22 Circolo Micologico ‘‘Giovanni Carini’’, C.P. 314, Brescia, Italy 23 Società per gli Studi Naturalistici della Romagna, C.P. 144, Bagnacavallo, RA, Italy 24 Department of Botany & Microbiology, H.N.B. Garhwal University, Srinagar, Garhwal, Uttarakhand 246174, India 123 Fungal Diversity (2016) 80:1–270 3 connections, DNA sequence data are also analysed from concatenated datasets (rDNA, TEF-a, RBP2 and b-Tubulin) to infer phylogenetic relationships and substantiate systematic position of taxa within appropriate ranks. Wherever new species or combinations are being proposed, we apply an integrative approach (morphological and molecular data as well as ecological features wherever applicable). Notes on 125 fungal taxa are compiled in this paper, including eight new genera, 101 new species, two new combinations, one neotype, four reference specimens, new host or distribution records for eight species and one alternative morphs. The new genera introduced in this paper are Alloarthopyrenia, Arundellina, Camarosporioides, Neomassaria, Neomassarina, Neotruncatella, Paracapsulospora and Pseudophaeosphaeria. The new species are Alfaria spartii, Alloarthopyrenia italica, Anthostomella ravenna, An. thailandica, Arthrinium paraphaeospermum, Arundellina typhae, Aspergillus koreanus, Asterina cynometrae, Bertiella ellipsoidea, Blastophorum aquaticum, Cainia globosa, Camarosporioides phragmitis, Ceramothyrium menglunense, Chaetosphaeronema achilleae, Chlamydotubeufia helicospora, Ciliochorella phanericola, Clavulinopsis aurantiaca, Colletotrichum insertae, Comoclathris italica, Coronophora myricoides, Cortinarius fulvescentoideus, Co. nymphatus, Co. pseudobulliardioides, Co. tenuifulvescens, Cunninghamella gigacellularis, Cyathus pyristriatus, Cytospora cotini, Dematiopleospora alliariae, De. cirsii, Diaporthe aseana, Di. garethjonesii, Distoseptispora multiseptata, Dis. tectonae, Dis. tectonigena, Dothiora buxi, Emericellopsis persica, Gloniopsis calami, Helicoma guttulatum, Helvella floriforma, H. oblongispora, Hermatomyces subiculosa, Juncaceicola italica, Lactarius dirkii, Lentithecium unicellulare, Le. voraginesporum, Leptosphaeria cirsii, Leptosphaeria irregularis, Leptospora galii, Le. thailandica, Lindgomyces pseudomadisonensis, Lophiotrema bambusae, Lo. fallopiae, Meliola citri-maximae, Minimelanolocus submersus, Montagnula cirsii, Mortierella fluviae, Muriphaeosphaeria ambrosiae, Neodidymelliopsis ranunculi, Neomassaria fabacearum, Neomassarina thailandica, Neomicrosphaeropsis cytisi, Neo. cytisinus, Neo. minima, Neopestalotiopsis cocoës, Neopestalotiopsis musae, Neoroussoella lenispora, Neotorula submersa, Neotruncatella endophytica, Nodulosphaeria italica, Occultibambusa aquatica, Oc. chiangraiensis, Ophiocordyceps hemisphaerica, Op. lacrimoidis, Paracapsulospora metroxyli, Pestalotiopsis sequoiae, Peziza fruticosa, Pleurotrema thailandica, Poaceicola arundinis, Polyporus mangshanensis, Pseudocoleophoma typhicola, Pseudodictyosporium thailandica, Pseudophaeosphaeria rubi, Purpureocillium sodanum, Ramariopsis atlantica, Rhodocybe griseoaurantia, Rh. indica, Rh. luteobrunnea, Russula indoalba, Ru. pseudoamoenicolor, Sporidesmium aquaticivaginatum, Sp. olivaceoconidium, Sp. pyriformatum, 34 Botanic Garden and Botanical Museum, Königin-LuiseStrasse 6-8, 14195 Berlin, Germany 43 35 Department of Life Sciences and Systems Biology, University of Torino, Viale P.A. Mattioli 25, 10125 Turin, Italy International Fungal Research and Development Centre, Key Laboratory of Resource Insect Cultivation & Utilization State Forestry Administration, The Research Institute of Resource Insects, Chinese Academy of Forestry, Kunming 650224, People’s Republic of China 36 Via Novaluce 38, 95125 Tremestieri Etneo, Catania, Italy 44 37 Via Giuseppe Mazzini 21, 25086 Rezzato, Brescia, Italy Botanical Museum, University of Helsinki, P.O. Box 7, 00014 Helsinki, Finland 45 38 Institute of Microbiology, Beijing Forestry University, Beijing 100083, People’s Republic of China Department of Biosciences, Plant Biology, University of Helsinki, P.O. Box 65, 00014 Helsinki, Finland 46 39 Division of Food Technology, Biotechnology & Agrochemistry, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, Republic of Korea Department of Plant Anatomy, Institute of Biology, Eötvös Loránd University, Pázmány Péter sétány 1/c, 1117 Budapest, Hungary 47 MushroomHobby.com, 345 Shipwatch Lane, Hercules, CA 94547, USA 48 Department of Biology, University of Washington, Box 351800, Seattle, WA 98195-1800, USA 49 Jodrell Laboratory, Royal Botanic Gardens, Kew TW9 3AB, UK 50 Laboratório de Biologia Molecular e Computacional de Fungos (LBMCF), Departamento de Microbiologia, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, Pampulha, Belo Horizonte, MG CEP: 31270-901, Brazil 51 Microorganisms Bank, Iranian Biological Resource Center (IBRC), Academic Center for Education, Culture & Research (ACECR), Tehran, Iran 40 41 42 Programa de Pós-Graduação em Biologia de Fungos, Departamento de Micologia, Universidade Federal de Pernambuco, Av. Prof. Moraes Rego, s/n, Recife, PE 50670-420, Brazil Departamento de Biologia Campus Universitário, Universidade Estadual de Maringá, Av. Colombo 5790, Jardim Universitario, Maringá, PR CEP 87020-900, Brazil Micolab, Programa de Pós-Graduação em Biologia de Fungos, Algas e Plantas, Departamento de Botânica, Universidade Federal de Santa Catarina, Campus Universitário Trindade, Florianópolis, SC CEP: 88040-900, Brazil 123 4 Fungal Diversity (2016) 80:1–270 Stagonospora forlicesenensis, Stagonosporopsis centaureae, Terriera thailandica, Tremateia arundicola, Tr. guiyangensis, Trichomerium bambusae, Tubeufia hyalospora, Tu. roseohelicospora and Wojnowicia italica. New combinations are given for Hermatomyces mirum and Pallidocercospora thailandica. A neotype is proposed for Cortinarius fulvescens. Reference specimens are given for Aquaphila albicans, Leptospora rubella, Platychora ulmi and Meliola pseudosasae, while new host or distribution records are provided for Diaporthe eres, Di. siamensis, Di. foeniculina, Dothiorella iranica, Do. sarmentorum, Do. vidmadera, Helvella tinta and Vaginatispora fuckelii, with full taxonomic details. An asexual state is also reported for the first time in Neoacanthostigma septoconstrictum. This paper contributes to a more comprehensive update and improved identification of many ascomycetes and basiodiomycetes. 367. Asterina cynometrae Hongsanan & K.D. Hyde, in Fungal Diversity 81: 12 (2016), new species Keywords Ascomycota Basidiomycota Dothideomycetes Mortierellomycotina Mucoromycotina Pezizomycetes Phylogeny Taxonomy New genus New species New host records 371. Pallidocercospora thailandica (Crous et al.) Phookamsak, Wulandari & K.D. Hyde, in Fungal Diversity 81: 21 (2016), new combination Table of contents Ascomycota Dothideomycetes Asterinales M.E. Barr ex D. Hawksw. & O.E. Erikss. Asterinaceae Hansf. 52 National Laboratory of Industrial Microbiology, Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran 53 Department of Molecular and Cellular Biology, Faculty of Basic Sciences and Advanced Technologies in Biology, University of Science and Culture, Tehran, Iran 54 Extremophiles Laboratory, Department of Microbiology, Faculty of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, Tehran, Iran 55 Department of Health Sciences, Faculty of Science, University of Mauritius, Reduit, Mauritius 56 Departamento de Micologia Avenida da Engenharia, Centro de Ciências Biológicas (CCB), Universidade Federal de Pernambuco (UFPE), S/N - Cidade Universitária, Recife, PE 50740-600, Brazil 57 Royal Botanic Gardens, Kew, Richmond, Surrey TW9 3DS, UK 58 Department of Botany, University of Calicut, Malappuram, Kerala 673 635, India 59 Laboratoire Interdisciplinaire des Energies de Demain, Université Paris Diderot, 35, Rue Hélène Brion, 75205 Paris, France 123 Botryosphaeriales C.L. Schoch et al. Botryosphaeriaceae Theiss. & Syd. 368. Dothiorella iranica Abdollahz. et al., in Abdollahzadeh et al., Persoonia, Mol. Phyl. Evol. Fungi 32: 4 (2014), new host record, p 15 369. Dothiorella sarmentorum (Fr.) A.J.L. Phillips et al., Mycologia 97: 522. 2005, new host record, p 17 370. Dothiorella vidmadera W.M. Pitt et al., Fungal Diversity 61 (1): 216 (2013), new host record, p 17 Capnodiales Woron. Mycosphaerellaceae Lindau Dothideales Lindau Dothideaceae Chevall. 372. Dothiora buxi Jayasiri, Camporesi & K.D. Hyde, in Fungal Diversity 81: 30 (2016), new species Hysteriales Lindau Hysteriaceae Chevall. 373. Gloniopsis calami Konta. & K.D. Hyde, in Fungal Diversity 81: 34 (2016), new species Dictyosporiaceae Boonmee & K.D. Hyde 374. Pseudocoleophoma typhicola E.B.G. Jones, Kamolhan, Boonmee & K.D. Hyde, in Fungal Diversity 81: 34 (2016), new species 375. Pseudodictyosporium thailandica C.G. Lin, Yong Wang bis & K.D. Hyde, in Fungal Diversity 81: 37 (2016), new species Pleosporales Luttr. ex M.E. Barr Didymellaceae Gruyter et al. 376. Neomicrosphaeropsis cytisi W.J. Li, Camporesi & K.D. Hyde, in Fungal Diversity 81: 38 (2016), new species 377. Neomicrosphaeropsis cytisinus Tennakoon, Camporesi & K.D. Hyde, in Fungal Diversity 81: 39 (2016), new species 378. Neomicrosphaeropsis minima W.J. Li, Camporesi & K.D. Hyde, in Fungal Diversity 81: 39 (2016), new species Fungal Diversity (2016) 80:1–270 379. Neodidymelliopsis ranunculi W.J. Li, Camporesi & K.D. Hyde, in Fungal Diversity 81: 41 (2016), new species 380. Platychora ulmi (J. Schröt.) Petr., Annls mycol. 23(1/2): 103 (1925), reference specimen, p 41 381. Stagonosporopsis centaureae Tennakoon, Camporesi & K.D. Hyde, in Fungal Diversity 81: 43 (2016), new species Didymosphaeriaceae Munk 382. Montagnula cirsii Qing Tian, Camporesi & K.D. Hyde, in Fungal Diversity 81: 43 (2016), new species 383. Tremateia arundicola Wanasinghe, E.B.G. Jones & K.D. Hyde, in Fungal Diversity 81: 45 (2016), new species 384. Tremateia guiyangensis J.F. Zhang, J.K. Liu, K.D. Hyde & Z.Y. Liu in Fungal Diversity 81: 48 (2016), new species Lentitheciaceae Y. Zhang et al. 5 Lophiotremataceae K. Hiray. & Kaz. Tanaka 393. Hermatomyces mirum (Starbäck) C.G. Lin, Yong Wang bis & K.D. Hyde, in Fungal Diversity 81: 69 (2016), new combination 394. Hermatomyces subiculosa C.G. Lin, Yong Wang bis & K.D. Hyde, in Fungal Diversity 81: 73 (2016), new species 395. Lophiotrema bambusae Phookamsak, S.C. Karunarathana & K.D. Hyde, in Fungal Diversity 81: 73 (2016), new species 396. Lophiotrema fallopiae A. Hashim. & Kaz. Tanaka, in Fungal Diversity 81: 74 (2016), new species Massariaceae Nitschke 397. Neomassaria Mapook, Camporesi & K.D. Hyde, in Fungal Diversity 81: 77 (2016), new genus 398. Neomassaria fabacearum Mapook, Camporesi & K.D. Hyde, in Fungal Diversity 81: 77 (2016), new species 385. Lentithecium unicellulare Abdel-Aziz, in Fungal Diversity 81: 53 (2016), new species 386. Lentithecium voraginesporum Abdel-Wahab, Bahkali & E.B.G. Jones, in Fungal Diversity 81: 53 (2016), new species 399. Stagonospora forlicesenensis Phukhamsakda, Camporesi & K.D. Hyde, in Fungal Diversity 81: 77 (2016), new species Leptosphaeriaceae M.E. Barr Melanommataceae G. Winter 387. Leptosphaeria cirsii Jayasiri, Camporesi & K.D. Hyde, in Fungal Diversity 81: 55 (2016), new species 388. Leptosphaeria irregularis R.H. Perera, E.B.G. Jones & K.D. Hyde, in Fungal Diversity 81: 59 (2016), new species 400. Bertiella ellipsoidea Ekanayaka, Q. Zhao & K.D. Hyde, in Fungal Diversity 81: 79 (2016), new species Lindgomycetaceae K. Hiray. et al. 389. Arundellina Wanasinghe, E.B.G. Jones & K.D. Hyde, in Fungal Diversity 81: 59 (2016), new genus 390. Arundellina typhae Wanasinghe, E.B.G. Jones & K.D. Hyde, in Fungal Diversity 81: 61 (2016), new species 391. Lindgomyces pseudomadisonensis Tak. Takah. & Kaz. Tanaka, in Fungal Diversity 81: 61 (2016), new species Lophiostomataceae Sacc. 392. Vaginatispora fuckelii (Sacc.) Thambugala, Wanasinghe, Kaz. Tanaka & K.D. Hyde, Fungal Diversity 74: 242. 2015, new host record, p 62 Massarinaceae Munk Occultabambusaceae Dai et al. 401. Occultibambusa aquatica Huang Zhang & K.D. Hyde, in Fungal Diversity 81: 81 (2016), new species 402. Occultibambusa chiangraiensis Phukhamsakda & K.D. Hyde, in Fungal Diversity 81: 81 (2016), new species Phaeosphaeriaceae M.E. Barr 403. Camarosporioides W.J. Li & K.D. Hyde, in Fungal Diversity 81: 83 (2016), new genus 404. Camarosporioides phragmitis W.J. Li & K.D. Hyde, in Fungal Diversity 81: 85 (2016), new species 405. Chaetosphaeronema achilleae S.K. Huang & K.D. Hyde, in Fungal Diversity 81: 85 (2016), new species 406. Dematiopleospora alliariae Thambugala, Camporesi & K.D. Hyde, in Fungal Diversity 81: 89 (2016), new species 123 6 Fungal Diversity (2016) 80:1–270 407. Dematiopleospora cirsii Wanasinghe, Camporesi, E.B.G. Jones & K.D. Hyde, in Fungal Diversity 81: 89 (2016), new species 408. Juncaceicola italica Tibpromma, Camporesi & K.D. Hyde, in Fungal Diversity 81: 93 (2016), new species 409. Leptospora rubella (Pers.) Rabenh., Klotzschii Herb. Viv. Mycol., Edn 2: no. 532 (1857), reference specimen, p 93 410. Leptospora galii de Silva & K.D. Hyde, in Fungal Diversity 81: 96 (2016), new species 411. Leptospora thailandica Phukhamsakda & K.D. Hyde, in Fungal Diversity 81: 100 (2016), new species 412. Muriphaeosphaeria ambrosiae S.K. Huang & K.D. Hyde, in Fungal Diversity 81: 104 (2016), new species 413. Nodulosphaeria italica Phookamsak, Camporesi & K.D. Hyde, in Fungal Diversity 81: 106 (2016), new species 414. Poaceicola arundinis W.J. Li, Camporesi, D.J. Bhat & K.D. Hyde, in Fungal Diversity 81: 111 (2016), new species 415. Pseudophaeosphaeria Jayasiri, Camporesi & K.D. Hyde, in Fungal Diversity 81: 111 (2016), new genus 416. Pseudophaeosphaeria rubi Jayasiri, Camporesi & K.D. Hyde, in Fungal Diversity 81: 112 (2016), new species 417. Wojnowicia italica Qing Tian, Camporesi & K.D. Hyde, in Fungal Diversity 81: 112 (2016), new species 423. Helicoma guttulatum Y.Z. Lu, Boonmee & K.D. Hyde, in Fungal Diversity 81: 125 (2016), new species 424. Neoacanthostigma septoconstrictum (Promp. & A.N. Mill.) S. Boonmee & K.D. Hyde, Fungal Diversity 68(1): 279 (2014), reference specimen, 125 425. Tubeufia hyalospora Y.Z. Lu, Boonmee & K.D. Hyde, in Fungal Diversity 81: 126 (2016), new species 426. Tubeufia roseohelicospora Y.Z. Lu, Boonmee & K.D. Hyde, in Fungal Diversity 81: 128 (2016), new species Pleosporaceae Nitschke 418. Comoclathris italica Tibpromma, Camporesi & K.D. Hyde, in Fungal Diversity 81: 117 (2016), new species 430. Neomassarina Phookamsak & K.D. Hyde, in Fungal Diversity 81: 136 (2016), new genus 431. Neomassarina thailandica Phookamsak & K.D. Hyde, in Fungal Diversity 81: 138 (2016), new species Roussoellaceae J.K. Liu et al. Eurotiomycetes 419. Neoroussoella lenispora J.F. Zhang, J.K. Liu, K.D. Hyde & Z.Y. Liu, in Fungal Diversity 81: 119 (2016), new species Eurotiales G.W. Martin ex Benny & Kimbr. Dothideomycetes family, incertae sedis Pleurotremataceae K.D. Hyde et al. 427. Pleurotrema thailandica Dayarathne, Jones E.B.G. & K.D. Hyde, in Fungal Diversity 81: 131 (2016), new species Trypetheliaceae Eschw. 428. Alloarthopyrenia Phukhamsakda, Lücking & K.D. Hyde, in Fungal Diversity 81: 131 (2016), new genus 429. Alloarthopyrenia italica Phukhamsakda, Camporesi, Ariyawansa & K.D. Hyde, in Fungal Diversity 81: 135 (2016), new species Pleosporales genera incetae sedis Torulaceae Corda 432. Aspergillus koreanus Hyang B. Lee, T.T. Duong & T.T.T. Nguyen, in Fungal Diversity 81: 142 (2016), new species 420. Neotorula submersa Z.L. Luo, H.Y. Su & K.D. Hyde, in Fungal Diversity 81: 121 (2016), new species Chaetothyriales M.E. Barr Chaetothyriaceae Hansf. ex M.E. Barr Tubeufiales Boonmee & K.D. Hyde Tubeufiaceae M.E. Barr 433. Ceramothyrium menglunense Mapook, J.F. Li & K.D. Hyde, in Fungal Diversity 81: 142 (2016), new species 421. Aquaphila albicans Goh, K.D. Hyde & W.H. Ho, Mycol. Res. 102(5): 588 (1998), reference specimen, p 121 422. Chlamydotubeufia helicospora Boonmee, Y. Z. Lu & K.D. Hyde, in Fungal Diversity 81: 123 (2016), new species Herpotrichiellaceae 123 434. Minimelanolocus submersus Z.L. Luo, H.Y. Su & K.D. Hyde, in Fungal Diversity 81: 143 (2016), new species Fungal Diversity (2016) 80:1–270 7 Trichomeriaceae Chomnunti & K.D. Hyde Valsaceae Tul. & C. Tul. 435. Trichomerium bambusae Hongsanan & K.D. Hyde, in Fungal Diversity 81: 145 (2016), new species 447. Cytospora cotini Norphanphoun, Bulgakov & K.D. Hyde, in Fungal Diversity 81: 176 (2016), new species Leotiomycetes Rhytismatales M.E. Barr ex Minter Rhytismataceae Chevall. Glomerellales Chadef. ex Réblová et al. Glomerellaceae Locq. 436. Terriera thailandica Jayasiri & K.D. Hyde, in Fungal Diversity 81: 146 (2016), new species Pezizomycetes Pezialaes J. Schrot. Helvelaceae Fr. 437. Helvella tinta Q. Zhao, B. Feng & K.D. Hyde, in Fungal Diversity 81: 149 (2016), new species 438. Helvella floriforma Q. Zhao & K.D. Hyde, in Fungal Diversity 81: 154 (2016), new species 439. Helvella oblongispora Harmaja, Karstenia 18(2): 57 (1978), new distribution record, p 157 Pezizaceae Dumort. 440. Peziza fruticosa Lantieri, Medardi & Vizzini, in Fungal Diversity 81: 157 (2016), new species Sordariomycetes Coronophorales Nannf. Coronophoraceae Höhn. 448. Colletotrichum insertae Jayawardena, Bulgakov & K.D. Hyde, in Fungal Diversity 81: 176 (2016), new species Reticulascaceae Réblová & W. Gams 449. Blastophorum aquaticum Z.L. Luo, Bhat, H.Y. Su & K.D. Hyde, in Fungal Diversity 81: 177 (2016), new species Hypocreales Lindau Ophiocordycipitaceae G.H. Sung et al. 450. Ophiocordyceps hemisphaerica Mafalda-Freire, Reck & Drechsler-Santos, in Fungal Diversity 81: 181 (2016), new species 451. Ophiocordyceps lacrimoidis Mafalda-Freire, Reck & Drechsler-Santos, in Fungal Diversity 81: 186 (2016), new species 452. Purpureocillium sodanum Papizadeh, Soudi, Wijayaw., Shahz.-Faz. & K.D. Hyde, in Fungal Diversity 81: 186 (2016), new species Hypocreales genus incertae sedis 441. Coronophora myricoides H.X. Wu & K.D. Hyde, in Fungal Diversity 81: 164 (2016), new species Diaporthales Nannf. Diaporthaceae Höhn. ex Wehm. Diaporthe Nitschke 442. Diaporthe aseana Dissanayake, Tangthirasunun & K.D. Hyde, in Fungal Diversity 81: 167 (2016), new species 443. Diaporthe eres Nitschke, Pyrenomycetes Germanici 2: 245 (1870), new host record, 167 444. Diaporthe foeniculina Niessl, in von Thümen, Contr. Ad. Fl. Myc. Lusit. 2: 30. 1880. new record, p 169 445. Diaporthe garethjonesii Dissanayake, Tangthirasunun & K.D. Hyde, in Fungal Diversity 81: 171 (2016), new species 446. Diaporthe siamensis Udayanga et al., Cryptogamie Mycologie 33: 298 (2012), new host record, p 171 453. Alfaria spartii Senan., Camporesi & K.D. Hyde, in Fungal Diversity 81: 187 (2016), new species Bionectriaceae Samuels & Rossman 454. Emericellopsis persica Papizadeh, Wijayaw, Soudi & K.D. Hyde, in Fungal Diversity 81: 191 (2016), new species Meliolales Gäum. ex D. Hawksw. & O.E. Erikss. Meliolaceae G.W. Martin ex Hansf. 455. Meliola citri-maximae X.Y. Zeng, K.D. Hyde & T.C. Wen, in Fungal Diversity 81: 192 (2016), new species 456. Meliola pseudosasae I. Hino, Bull. Faculty of Agriculture, Yamaguchi University 9: 882 (1958), reference specimen, p 196 123 8 Xylariales Nannf. Apiosporaceae K.D. Hyde et al. 457. Arthrinium paraphaeospermum Senan. & K.D. Hyde, in Fungal Diversity 81: 198 (2016), new species Bartaliniaceae Wijayaw. et al. 458. Neotruncatella Hyang B. Lee & T.T.T. Nguyen, in Fungal Diversity 81: 198 (2016), new genus 459. Neotruncatella endophytica Hyang B. Lee, P.M. Kirk, K.D. Hyde, S.S.N. Maharachch, & T.T.T. Nguyen, in Fungal Diversity 81: 198 (2016), new species Fungal Diversity (2016) 80:1–270 Distoseptisporaceae Su et al. 470. Distoseptispora multiseptata J. Yang & K.D. Hyde, in Fungal Diversity 81: 220 (2016), new species 471. Distoseptispora tectonae Doilom & K.D. Hyde, in Fungal Diversity 81: 222 (2016), new species 472. Distoseptispora tectonigena Doilom & K.D. Hyde, in Fungal Diversity 81: 222 (2016), new species Sordariomycetidae, Incertae sedis 473. Paracapsulospora Konta & K.D. Hyde, in Fungal Diversity 81: 223 (2016), new genus 474. Paracapsulospora metroxyli Konta & K.D. Hyde, in Fungal Diversity 81: 223 (2016), new species Cainiaceae J.C. Krug 460. Cainia globosa Senan., Camporesi & K.D. Hyde, in Fungal Diversity 81: 201 (2016), new species Basidiomycota Agaricomycetes Agaricales Underw. Clavariaceae Chevall. Pestalotiopsidaceae Maharachch. & K.D. Hyde 461. Ciliochorella phanericola Norphanphoun, T.C. Wen & K.D. Hyde, in Fungal Diversity 81: 207 (2016), new species 462. Neopestalotiopsis cocoës Norphanphoun, T.C. Wen & K.D. Hyde, in Fungal Diversity 81: 207 (2016), new species 463. Neopestalotiopsis musae Norphanphoun, T.C. Wen & K.D. Hyde, in Fungal Diversity 81: 209 (2016) 464. Pestalotiopsis sequoiae W.J. Li, Camporesi & K.D. Hyde, in Fungal Diversity 81: 210 (2016), new species Xylariaceae Tul. & C. Tul. 465. Anthostomella ravennica Daranagama, Camporesi & K. D. Hyde, in Fungal Diversity 81: 210 (2016), new species 466. Anthostomella thailandica Daranagama & K.D. Hyde, in Fungal Diversity 81: 213 (2016) Sordariomycetes incertae sedis Sporidesmiaceae Fr. 467. Sporidesmium pyriformatum J. Yang & K.D. Hyde, in Fungal Diversity 81: 215 (2016), new species 468. Sporidesmium aquaticivaginatum J. Yang & K.D. Hyde, in Fungal Diversity 81: 217 (2016), new species 469. Sporidesmium olivaceoconidium J. Yang & K.D. Hyde, in Fungal Diversity 81: 220 (2016), new species 123 475. Clavulinopsis aurantiaca Araujo-Neta, Silva & Gibertoni, in Fungal Diversity 81: 225 (2016), new species 476. Ramariopsis atlantica Araujo-Neta, Silva & Gibertoni, in Fungal Diversity 81: 226 (2016), new species Cortinariaceae R. Heim ex Pouzar 477. Cortinarius fulvescens Fr., Epicr. syst. mycol. (Upsaliae): 311 (1838), neotype, 227 478. Cortinarius fulvescentoideus Kytöv., Niskanen & Liimat., in Fungal Diversity 81: 230 (2016), new species 479. Cortinarius nymphatus Kytöv., Niskanen, Liimat. & Bojantchev, in Fungal Diversity 81: 230 (2016), new species 480. Cortinarius pseudobulliardioides Kytöv., Niskanen, Liimat. & Ammirati, in Fungal Diversity 81: 232 (2016), new species 481. Cortinarius tenuifulvescens Kytöv., Niskanen & Liimat., in Fungal Diversity 81: 232 (2016), new species Entolomataceae Kotl. & Pouzar 482. Rhodocybe indica K.N.A. Raj & Manim., in Fungal Diversity 81: 236 (2016), new species 483. Rhodocybe luteobrunnea K.N.A. Raj & Manim., in Fungal Diversity 81: 241 (2016), new species 484. Rhodocybe griseoaurantia K.N.A. Raj & Manim., in Fungal Diversity 81: 242 (2016), new species Fungal Diversity (2016) 80:1–270 Agaricaceae Chevall. 485. Cyathus pyristriatus B. Thongbai, C. Richt. & M. Stadler, in Fungal Diversity 81: 244 (2016), new species Polyporales Gäum. Polyporaceae Fr. ex Corda 486. Polyporus mangshanensis B.K. Cui, J.L. Zhou & Y.C. Dai, in Fungal Diversity 81: 249 (2016), new species Russulales Kreisel ex P.M. Kirk et al. Russulaceae Lotsy 487. Russula indoalba A. Ghosh, Buyck, A. Baghela, K. Das & R.P. Bhatt, in Fungal Diversity 81: 250 (2016), new species 488. Russula pseudoamoenicolor A. Ghosh, Buyck, K. Das, A. Baghela & R.P. Bhatt, in Fungal Diversity 81: 251 (2016) 489. Lactarius dirkii Uniyal, K. Das, A. Baghela & R.P. Bhatt, in Fungal Diversity 81: 252 (2016), new species Mortierellomycotina Kerst. Hoffm. et al. Mortierellales Caval.-Sm. Mortierellaceae A. Fisch. Mortierella fluviae Hyang B. Lee, K. Voigt & T.T.T. Nguyen, in Fungal Diversity 81: 254 (2016), new species Mucorales Dumort. Cunninghamellaceae Naumov ex R.K. Benj. 490. Cunninghamella gigacellularis A.L. Santiago, C.L. Lima & C.A. de Souza, in Fungal Diversity 81: 255 (2016), new species Materials and methods Sampling, isolation and identification Specimens from a number of plants examined in this study were collected from at least 15 countries around the world, including Brazil, China, Egypt, Germany, Iran, India, Italy, Japan, Philippines, Republic of Korea, Russia, Saudi Arabia, Thailand and the UK. Soil samples collected from the Atlantic rainforest in Recife, Brazil to isolate Cunninghamella gigacellularis followed methods outlined by Benny (2008). The color designation of C. gigacellularis colonies was established according to Maerz and Paul (1950). Samples were observed under the microscope; macro-morphological (e.g. from basidiomycetes) and micro-morphological characters (e.g. 9 ascomata sections, peridium structures, asci and ascospores) were examined, described and photographed. Most measurements (e.g. from basidiospores and ascospores) were taken from at least 20 representatives and both the mean and the standard deviation for both the length and the width, together with the range of spore quotient (Q, the length/width ratio) and its mean value (Qm) are given. Axenic cultures (following single spore isolations) and specimens with duplicates have been deposited in Culture Collections and Herbaria where appropriate and accession numbers are provided in the taxonomic descriptions. Faces of Fungi and Index Fungorum numbers are given as outlined by Jayasiri et al. (2015) and Index Fungorum (2016). Wherever possible, appropriate techniques were employed to induce the formation of the asexual morph in culture using sterilized pieces of plant materials. Colony characters were observed and recorded. We have tried to maintain consistency in terminology, however this may not always be possible as different authors prefer to use different terms. For example the use of ascostromata is variable and often difficult to interpretate and is left to each author’s discretion. DNA extraction, PCR amplification and sequencing For most fungal samples (especially ascomycetes), total genomic DNA was extracted from fresh fungal mycelium grown on appropriate media agar at room temperature with appropriate Genomic DNA Extraction Kit purchased from countries where samples were collected following manufacturer’s instructions. Under circumstances where fungi fail to grow in culture, DNA was extracted directly from fruiting bodies using aseptic techniques. The procedure described by Izumitsu et al. (2012) was employed for extracting genomic DNA from dried Rhodocybe specimens. For Cunninghamella isolates, genomic DNA extraction was carried out with macerated material according to Góes-Neto et al. (2005). DNA amplification for most samples was performed by polymerase chain reaction (PCR) using universally standard primers such as LROR and LR5 (Vilgalys and Hester 1990) for the partial large subunit nuclear rDNA (28S, LSU); NS1 and NS4 (White et al. 1990) for the small subunit nuclear rDNA (18S, SSU); ITS4 and ITS5 (White et al. 1990) for the internal transcribed spacers (5.8S, ITS); EF1-983F and EF1-2218R (Rehner 2001) for the translation elongation factor 1-alpha gene (TEF1a); and fRPB25F and fRPB2-7cR (Liu et al. 1999) for the partial RNA polymerase second largest subunit (RPB2). Primer pair LR1/LSU2 were used to amplify LSU region of Cunninghamella isolates (van Tuinen et al. 1998) while LROR/ LR7 for (Vilgalys and Hester 1990) and b6Fb7.1R 123 10 Fungal Diversity (2016) 80:1–270 Abrothallales Abrothallus parmotrematis AB1 Melaspileopsis diplasiospora Ertz 16247 88/1.0 Melaspileopsis sp. Ertz 17904 100/1.0 Stictographa lentiginosa Ertz 17570 100/1.0 Stictographa lentiginosa Ertz 17447 66/0.9 Karschia talcophila 16749 100/1.0 Karschia cezannei Cezanne-Eichler 7453 95/1.0 100/1.0 Buelliella physciicola Ertz 19173 Buelliella physciicola Ertz 18113 /0.9 Labrocarpon canariense 16907 99/1.0 Labrocarpon canariense 16308 Asterina zanthoxyli TH 561 100/1.0 Asterina fuchsiae TH 590 Asterinales Asterina weinmanniae TH 592 Asterina sp. MFU13-0619 94/1.0 Asterina cestricola TH 591 50/ Asterina phenacis TH 589 64/0.89 Asterina siphocampyli ppMP 1324 66/0.9 Asterina cynometae MFLU 13–0373 100/1.0 Inocyclus angularis VIC 39747 Lembosia albersii MFU13-0377 100/1.0 Lembosia xyliae MFLU14-0004 100/1.0 Buelliella poetschii Ertz 18115 100/1.0 Buelliella poetschii Ertz 18116 Asterotexis cucurbitacearum PMA M-0141224 Parmularia styracis VIC 42587 100/1.0 Parmularia styracis VIC 424477 Prillieuxina baccharidincola VIC 42817 98/1.0 Incertae sedis clade Asterina melastomatis VIC 42822 100/1.0 Asterina chrysophylli VIC 42823 96/1.0 Lembosia abaxialis VIC 42825 99/1.0 54/0.9 Batistinula sp. MFLU 13-0514 51/0.89 Batistinula gallesiae VIC 42514 Coniosporium uncinatum CBS 100212 Capronia munkii AFTOL-ID 656 0.1 Fig. 1 Phylogram generated from maximum likelihood and Bayesian analyses based on LSU sequence data from species of Asterinales and Asterotexales. The first set of numbers above the nodes are RAxML bootstrap value expressed from 1000 repetitions with values above 50 % shown. The second set of numbers above the nodes are Bayesian posterior probabilities, with values above 0.85 shown. The new isolates are in blue bold and other ex-type strains are in bold. The tree is rooted with Capronia munkii Unter (Matheny 2005) for RPB2 region were used for Rhodocybe specimens. Thermal cycle program for ITS, LSU and TEF1a amplification were as follows: initial 94 °C for 3 mins, followed by 35 cycles of denaturation at 94 °C for 30 s, annealing at 55 °C for 50 s, elongation at 72 °C for 1 mins, and final extension at 72 °C for 10 mins. For ascomycetes, the thermal cycle program for RPB2 was as follows: initial 95 °C for 5 mins, followed by 40 cycles of denaturation at 95 °C for 1 mins, annealing at 52 °C for 2 mins, elongation at 72 °C for 90 s, and final extension at 72 °C for 10 mins. Thermal cycle program for Cunninghamella isolates were follows: 95 °C for 5 mins, followed by 39 cycles of denaturation at 94 °C for 45 s, annealing at 60 °C for 1 min, elongation at 72 °C for 1 min, and final extension at 72 °C for 7 mins. For Rhodocybe specimens, thermal profiling and amplification reactions of ITS, nLSU and RPB2 regions were performed following Latha et al. (2015). Prior to sequencing, quality of PCR amplicons were checked and purified with appropriate purification kits as per manufacturer’s guidelines before being subjected to automated DNA sequencing using the same primers used for PCR. Sequence alignment and phylogenetic analyses 123 A careful verification of all sequences obtained were done especially with appropriate reference sequences following a Blast search in GenBank to ensure that no erroneous sequences are used in further analyses and then submitted to GenBank. Following sequence verification and Blast search, DNA sequences from appropriate taxonomic ranks were downloaded to construct datasets for phylogenetic analyses. BioEdit sequence alignment editor (Hall 1999), AliView v.1.17 (Larsson 2014), CLUSTALX (Larkin et al. 2007), Mega 6.0.5 (Tamura et al. 2013) and MAFFT: multiple sequence alignment software version 7.215 (Katoh et al. 2002) were used for alignment purposes. Under most circumstances, concatenated DNA datasets are analyzed to generate gene trees but in cases of limited availability of DNA sequences from respective gene regions, phylogenies are inferred from single or two genes datasets and either a consensus of these gene phylogenies or one of the most parsimonious phylogeny is used to infer phylogenetic relationships across taxa sampled. Selection of outgroup (s) for rooting purposes was based on Fungal Diversity (2016) 80:1–270 11 Fig. 2 Asterina cynometrae (holotype). a Appearance of thyriothecia on leaf. b, c Thyriothecia with star-like opening when viewed in squash mounts. d Superficial hyphae with hyphopodia. e Upper wall of thyriothecium, f Ascus in Melzer’s reagent. g Ascus at maturity. h, i Ascospores. Scale bars b, c = 100 lm, d, e = 20 lm, f–i = 10 lm knowledge of potential common ancestor to our in-group as well as taxon sampling from previously published studies. Phylogenetic analyses were performed by maximum parsimony (MP), maximum likelihood (RAxML) and Bayesian inference (BI) analyses. A maximum parsimony (MP) analysis was performed with stepwise additions of sequences by using PAUP v. 4.0b10 (Swofford 2002). The heuristic search option with 1000 random sequences addition and tree-bisection reconnection (TBR) of branch-swapping algorithm were 123 12 performed. Maxtrees were setup at 5000, branches of zero length were collapsed and gaps were treated as missing data. Tree length (TL), consistency index (CI), retention index (RI), rescaled consistency index (RC) and homoplasy index (HI) were calculated for trees generated under different optimality criteria. The robustness of the most parsimonious trees was evaluated by 1000 bootstrap replications resulting from maximum parsimony analysis with each 100 replicates of random stepwise addition of taxa (Liu et al. 2011, 2012). Maximum likelihood (ML) analysis was performed using RAxML v.8.0.26 (Stamatakis 2014) with 1000 rapid ML bootstrap replicates. The available substitution models comprised a generalized time reversible (GTR) for nucleotides was applied with a discrete gamma distribution (Silvestro and Michalak 2012). A discrete GAMMA (Yang 1994) was complemented for each substitution model. Rapid bootstrap analysis (Stamatakis et al. 2008) and search for a best-scoring ML tree were applied (Silvestro and Michalak 2012). The parameters were followed; run mode = ML ? rapid bootstrap, replicates = 1000, BS brL = selected and Model = GTRGAMMA. Bayesian analysis was performed by MrBayes v. 3.0b4 (Ronquist and Huelsenbeck 2003) with the best-fit model of sequence evolution estimated with MrModeltest 2.2 (Nylander et al. 2008). Markov Chain Monte Carlo sampling (BMCMC) was used to determine the posterior probabilities (PP) (Rannala and Yang 1996; Zhaxybayeva and Gogarten 2002) in MrBayes v. 3.0b4 (Huelsenbeck and Ronquist 2001). Six simultaneous Markov chains were run for 1,000,000 generations sampling one tree every 100th generations of trees (resulting 10,001 total trees). The burnin (first 2000 trees) which represented the phase of the analysis were discarded and the remaining 8000 trees were used to build a majority rule consensus tree (Liu et al. 2011, 2012) with posterior probabilities (PP). Phylograms were visualized in Treeview (Page 2001) or FigTree 1.4.2 (Rambaut 2014) with bootstrap values above or below the nodes. All the sequences generated in this study have been deposited in GenBank and accession numbers provided where appropriate. Dothideomycetes We follow Hyde et al. (2013) and Wijayawardene et al. (2014a) for the latest arrangement of this class. Asterinales M.E. Barr ex D. Hawksw. & O.E. Erikss. The order was monographed by Hongsanan et al. (2014). Guatimosim et al. (2015) provided sequence data for Asterina and Parmularia directly from ascomata, but did not include other sequence data used in Hofmann (2009). Ertz and Diederich (2015) provided sequence data for taxa of Melaspileaceae and placed them in Asterinales 123 Fungal Diversity (2016) 80:1–270 based on their phylogenetic data. However, Ertz et al. (2016) indicated that Asterina species are segregated in two unrelated clades. Asterotexis cucurbitacearum and Inocyclus angularia (Parmulariaceae) clustered with strains of Asterina provided by Hofmann (2009) and Hongsanan et al. (2014), and formed a sister group to Melaspileaceae (Ertz et al. 2016). Thus, these Asterinales strains were considered to represent the order Asterotexiales based on the type species of Asterotexis cucurbitacearum (Ertz et al. 2016). In the present study we treat the main Asterinales clade, which includes Asterotexis cucurbitacearum, as Asterinales sensu stricto because most of the Asterinales strains, from both Asterina and Lembosia cluster in this clade and also because the large clade supporting Asterinales as circumscribed by Ertz et al. (2016) does not have any phylogenetic support. We therefore synonymize the younger Asterotexiales (in December, 2015) under Asterinales (in 1986). It is questionable that the other clade, which contains the putatively named, type species of Asterina is actually Asterinales as most strains in this order cluster in Asterinales sensu stricto. It may be that DNA was amplified from other taxa in the black mildew colonies. Since Parmularia represents a distinct monophyletic clade with high support outside Asterinales sensu stricto, we reinstate Parmulariaceae to accommodate this clade (Fig. 1). Asterinaceae Hansf. The family Asterinaceae was established in Myriangiales by Hansford (1946). Several studies placed Asterinaceae in an uncertain position in the Dothideomycetes incertae sedis (Cannon and Kirk 2007; Kirk et al. 2008). Phylogenies of Hongsanan et al. (2014) place Asterinaceae within Asterinales in Dothideomycetes. They also accepted 17 genera in the family based mainly on morphology. In this paper, we introduce a new species, Asterina cynometrae with morphological details and molecular data (Figs. 1, 2). Asterina Lév. The genus Asterina is the type genus of the family Asterinaceae which was introduced by Léveillé (1845). Asterina is the largest genus in Asterinaceae, and has a worldwide distribution in tropical and subtropical regions (Hongsanan et al. 2014). According to Ertz et al. (2016) Asterina species cluster in two well-supported distinct clades, which are placed in the order Asterinales and in the incertae sedis clade in this study (Fig. 1). Asterina cynometrae Hongsanan & K.D. Hyde, sp. nov. Index Fungorum number: IF552216; Facesoffungi number: FoF02430, Fig. 2 Holotype: MFLU 13-0373. Fungal Diversity (2016) 80:1–270 13 Fig. 3 One of 309 most parsimonious trees obtained from combined ITS and EF-1a sequence data, for all ex-types from species in Dothiorella. Isolate numbers of new host records are in blue. Maximum parsimony bootstrap values ([70 %) and Bayesian inference values ([0.9) are given on the nodes. The tree is rooted with Spencermartinsia viticola Epiphytes on the upper surface of leaves. Superficial hyphae branched, septate, darker at the septum, brown, with hyphopodia. Hyphopodia 11–13 lm high 9 4–8 lm wide ( x = 12 9 6 lm, n = 20) capitate, alternate, rarely opposite on hyphae, near to hyphal septum, 1-celled, with 2 branches at the apex, brown. Sexual morph Thyriothecia 150–200 lm diam. ( x = 160 lm, n = 10), superficial on the surface of host, solitary to gregarious, circular, flattened, with star-like opening, sometimes variously shaped. Upper wall comprising parallel arrangement of cells radiating from the center, base poorly developed. Hamathecium not observed. Asci up to 40 lm diam., vertically arranged within thyriothecium cavity, 6-spored, bitunicate, fissitunicate, subglobose to globose, short pedicellate, 123 14 Fungal Diversity (2016) 80:1–270 Fig. 4 Dothiorella iranica (MFLU 15-1402). a–c Conidiomata on host surface. d Section through the conidioma. e Conidioma wall. f– i Conidiophore and conidiogenous cells. j–m Conidia. Scale bars d = 50 lm, e = 20 lm, f–m = 10 lm without an ocular chamber, with evanescent wall. Ascospores 18–22 lm 9 10–12 lm ( x = 20 9 11 lm, n = 15), 2–3-seriate, oblong, hyaline to dark brown, uniseptate at the middle, constricted and with dark band at the septum, smooth-walled, ends rounded. Asexual morph Undetermined. Material examined: PHILIPPINES, Luzon, Laguna Province, Mount Makiling, on living leaves of Cynometra sp. (Fabaceae), February 2012, Pamela Alva (MFLU 13-0373, holotype). Notes: Asterina cynometrae was collected on living leaves of Cynometra sp. (Fabaceae) and is most similar to A. trachycarpa Syd. & P. Syd. in the shape and size of ascospores, the latter species being found on Derris atroviolacea Elmer (Fabaceae) in the Philippines. However, it differs from A. trachycarpa in having a thick and dark band at the septum of ascospores, with 2–3 branching hyphopodia. We therefore introduce A. cynometrae as a new species based on host and morphology (Fig. 2). 123 Furthermore, we place our new species within Asterinales based on the phylogeny of LSU sequence data (Fig. 1). As we were unable to isolate the new taxon because of its obligate parasitic habitat, sequence data was prepared directly from thyriothecia and ascospores. Botryosphaeriales C.L. Schoch et al. The order Botryosphaeriales has undergone significant taxonomic changes during the past decade by addition of several new families. Currently, there are seven families, Aplosporellaceae, Botryosphaeriaceae, Melanopsaceae, Planistromellaceae, Phyllostictaceae, Saccharataceae and Septorioideaceae (Schoch et al. 2006; Minnis et al. 2012; Wikee et al. 2013; Slippers et al. 2013; Wyka and Broders 2016) and several new genera (Liu et al. 2012; Crous et al. 2015b). Botryosphaeriales is a diverse order with a worldwide distribution, comprising species that vary from endophytes to pathogens (Slippers and Wingfield 2007) and occurring on a wide range of monocotyledonous, Fungal Diversity (2016) 80:1–270 15 Fig. 5 Dothiorella sarmentorum (MFLU 16-1274). a Conidiomata on Morus alba. b Vertical section through a conidioma. c Peridium of conidioma. d–g Conidia attached to conidiogenous cells. h–l Mature and immature conidia. Scale bars a = 500 lm, b = 100 lm, c = 50 lm, d = 20 lm, e–l = 10 lm dicotyledonous, and gymnosperm hosts (Liu et al. 2012; Crous et al. 2015b) and lichens (Barr 1987; von Arx 1987). Many are considered as pathogens that cause disease on a wide range of economically and ecologically significant plants (Slippers et al. 2013). is almost impossible without the support of molecular data (Slippers et al. 2013). We provide an updated tree for the genus (Fig. 3). Dothiorella Sacc. The species in the genus Dothiorella (Botryosphaeriaceae, Botryosphaeriales, Dothideomycetes) is characterized based on conidia that become pigmented and 1-septate while they are still attached to the conidiogenous cells (Phillips et al. 2013). Due to wide host ranges and morphological plasticity, identification of species in this genus Dothiorella iranica Abdollahz. et al., in Abdollahzadeh et al., Persoonia, Mol. Phyl. Evol. Fungi 32: 4 (2014) Facesoffungi number: FoF02202, Fig. 4 Saprobic on Paliurus bark. Asexual morph Conidiomata 280–305 lm high, 275–310 lm diam. ( x= 290 9 295 lm, n = 5), acervular, solitary to gregarious, superficial to immersed, unilocular, globose to subglobose, dark brown to black. Conidiomata wall 30–40 lm wide, composed of brown, thin or thick-walled cells of textura 123 16 Fig. 6 Dothiorella vidmadera (MFLU 16-1273). a Robinia pseudoacacia with fungus. b Conidiomata on host substrate. c Vertical section through a conidioma. d Peridium of conidioma. e Conidium 123 Fungal Diversity (2016) 80:1–270 attached to conidiogenous cell. f–i Mature and immature conidia. Scale bars b = 500 lm, c = 100 lm, d = 20 lm, e–i = 10 lm Fungal Diversity (2016) 80:1–270 angularis, apex and base thicker than middle, with setae. Conidiophores 7–9 9 2–3 lm ( x = 8 9 2.5 lm, n = 10), cylindrical, filiform, septate, branched, hyaline. Conidiogenous cells 9–17 9 4–6 lm, ( x = 12 9 5 lm, n = 20), holoblastic, annellidic, integrated or discrete, hyaline, determinate. Conidia 20–25 9 8–11 lm ( x = 22 9 9 lm, n = 30), cylindrical, oval or ellipsoid, 1-septate, hyaline when immature, brown to dark brown when mature. Sexual morph Undetermined. Culture characteristics: Ascospores germinating on MEA within 36 h. Colonies growing on MEA attaining 2 cm diam. in 1 week at 28 °C. Mycelium superficial, felted, gummy, dark brown to black. Asexual structures not formed in culture. Material examined: ITALY, Province of Forlı̀-Cesena, Monte Pallareto—Meldola, on Paliurus bark (Rhamnaceae), 1 January 2012, Erio Camporesi IT 962 (MFLU 15-1402, KUN, new host record), living culture, MFLUCC 15-0656, KUNCC Notes: Phylogenetically this species resides in a distinct subclade in Dothiorella with high support and closely related to D. ulmacea (Fig. 3). The conidia of D. iranica are longer than those of all other Dothiorella species except D. casuarini J. de Wet et al. (27 9 11 lm). Our isolate is morphologically and phylogenetically similar to Dothiorella iranica (strain IRAN 1587C), but associated with a different host. Dothiorella iranica (type) was recorded on Olea europaea L. (Oleaceae) and our collection was found on Paliurus (Rhamnaceae) bark. Host distribution is poorly known in this genus, but according to Dissanayake et al. (2016), Dothiorella species may be host specific, although Dothiorella iberica A.J.L. Phillips et al., D. sarmentorum (Fr.) A.J.L. Phillips et al. and D. symphoricarposicola W.J. Li occur on many host families and orders. Dothiorella sarmentorum (Fr.) A.J.L. Phillips, J. Luque & A. Alves, Mycologia 97: 522. 2005 : Sphaeria sarmentorum Fr., K. svenska VetenskAcad. Handl. 39: 107. 1818. : Diplodia sarmentorum (Fr.) Fr., Summ. veg. Scand. (Stockholm) 2: 417. 1849. = Botryosphaeria sarmentorum A.J.L. Phillips, J. Luque & A. Alves, Mycologia 97: 522. 2005. Facesoffungi number: FoF02148, Fig. 5 Saprobic on Celtis occidentalis L. Sexual morph Undetermined. Asexual morph Conidiomata 140–240 lm high 9 175–300 lm diam. ( x = 230 9 180 lm, n = 10), stromatic, solitary or scattered in small groups, immersed, uni or biloculate, black, globose to subglobose, ostiolate. Conidiomatal wall 25–45 lm ( x = 35 lm, n = 15), comprising several layers; outer layers comprising thick-walled, dark brown, somewhat flattened cells of textura angularis and inner layers of larger, thin-walled, lightly 17 pigmented or hyaline cells. Conidiophores reduced to conidiogenous cells. Conidiogenous cells 6–12 9 2–4 lm ( x = 8.6 9 3.3 lm, n = 15), lining the conidiomatal cavity, holoblastic, hyaline, subcylindrical, proliferating at the same level giving rise to periclinal thickenings. Conidia 17.8–22.4 9 8.3–11 lm ( x = 20.2 9 9.9 lm, n = 30), ovoid, with a broadly rounded apex and truncate base, initially hyaline to lightly pigmented and aseptate, becoming dark brown and 1-septate, slightly constricted at the septum, smooth-walled. Culture characteristics: Conidia germinating on PDA within 18 h and germ tubes produced from one end or both cells. Colonies on PDA at 25 °C, covering 9 cm Petridishes in few days, circular, flat, dense, surface initially white, becoming grey with reverse black, smooth surface with entire to slightly undulate edge. Material examined: RUSSIA, Rostov Region, Shakhty City, former Shakhty forestry, on Celtis occidentalis L. (Cannabaceae), 26 February 2014, Timur Bulgakov T 35 (MFLU 16-1008, new host record), living culture MFLUCC 15-0443. RUSSIA, Rostov region, Shakhty city, Central Park (47.7055886°E, 40.2059913°N), on Morus alba (Moraceae), 12 March 2014, Timur S. Bulgakov T 07 (MFLU 16-1274, new host record), living culture, MFLUCC 14-0889, MUCL. Notes: Dothiorella sarmentorum was introduced by Phillips et al. (2005) based on the asexual morph of Botryosphaeria sarmentorum A.J.L. Phillips et al. This species has been recorded from 34 different host species (Phillips et al. 2005, 2013). The sexual morph of D. sarmentorum is characterized by partially erumpent ascomata with papillate ostiole, 4–6(–8)-spored asci and oblong to ovate, (0–)1-septate, finely verruculose ascospores, which are widest in the middle part (Phillips et al. 2013). As the morphological information does not provide any clear difference, we would like to report our collections as new host records of D. sarmentorum. This is the first report of Dothiorella sarmentorum on Celtis occidentalis and Morus alba from Russia. ITS and EF-1a based phylogenies also depict that all D. sarmentorum isolates cluster together and is phylogenetically related to D. americana (Fig. 3) Dothiorella vidmadera W.M. Pitt et al., Fungal Diversity 61: 216 (2013) Facesoffungi number: FoF02206, Fig. 6 Saprobic or weak pathogenic on twigs of Robinia pseudoacacia L. Sexual morph Undetermined. Asexual morph Conidiomata 240–280 lm high 9 280–320 lm diam. ( x = 263 9 298 lm, n = 10), pycnidial, stromatic, mostly solitary, semi-immersed to immersed in the host, globose, dark brown to black, ostiolate, apapillate. Peridium 20–30 lm wide at the base, 25–35 lm wide at the side, comprising 5–6 layers, heavily pigmented, thick- 123 18 123 Fungal Diversity (2016) 80:1–270 Fungal Diversity (2016) 80:1–270 b Fig. 7 Phylogram generated from maximum likelihood analysis (RAxML) based on combined ITS and LSU sequence data of respective genera in Mycosphaerellaceae. Bootstrap support values for maximum likelihood (ML, left) and maximum parsimony (MP, right) equal to or greater than 50 % are given above the nodes. The values of the Bayesian posterior probabilities from MCMC analyses (BYPP) equal or higher than 95 % are given below the nodes. The tree is rooted with Lecanosticta acicola (CBS 871.95). Ex-type and ex-epitype strains are in bold. The generated sequences in this study are indicated in blue walled, blackish to dark brown, angular cells, becoming flattened towards the outer layers. Conidiogenous cells 5– 9 lm high 9 2–4 lm wide, holoblastic, cylindrical to subcylindrical, hyaline, the first conidium produced holoblastically and subsequent conidia enteroblastically forming typical phialides with periclinal thickenings, swollen at the base, discrete, producing a single conidium at the apex. Conidia 18–22 9 8–10 lm ( x = 19.9 9 8.6 lm, n = 30), initially hyaline, unicellular, becoming dark brown and 1-septate while still attached to conidiogenous cells; detached conidia, hyaline, sepia or blackishbrown, unicellular or 1-septate, moderately thick-walled, wall externally smooth, roughened on the inner surface, oval to ovoid, widest in the center, apex obtuse, base truncate or rounded, guttulate when young. Culture characteristics: Colonies on MEA reaching 5 cm diam. after 30 days at 16 °C, circular, smooth margin, greyish-green to blackish-green after 28 days flat on the surface, without aerial mycelium, reverse greyishbrown to black. Hyphae septate branched, hyaline, thin, smooth-walled. Material examined: RUSSIA, Rostov region, Rostov-naDonu city, Botanical garden of Southern Federal University, Higher Park, underwood (47.2389405°E, 39.6484137°N), on Robinia pseudoacacia (Fabaceae), 26 March 2014, Timur S. Bulgakov T 06 (MFLU 16-1273, new host record), living culture, MFLUCC 14-0888, MUCL. Notes: In the combined phylogenetic analysis (ITS and EF1-a), MFLUCC 14-0888 strain is phylogenetically most closely related to D. vidmadera (Fig. 3). Our isolate resembles D. vidmadera in the shape and size of the conidia but our collection (MFLU 16-1273) has darker conidia than DAR78992 of Dothiorella vidmadera (Pitt et al. 2013). As the morphological information does not provide any clear divergence, we report our collection as a new host record of D. vidmadera. This species has been previously recorded only from Vitis vinifera and Fraxinus 19 ornus. This is the first record of Dothiorella vidmadera on Robinia species. Capnodiales Woron. The order Capnodiales comprises human pathogens, plant saprotrophs and rock-inhabiting species. This order was reviewed by Chomnunti et al. (2011) and in this paper we follow the recent publication of Hyde et al. (2013). Mycosphaerellaceae Lindau The family Mycosphaerellaceae was introduced by Lindau (1897) and typified by Mycosphaerella, with M. punctiformis (Pers.) Starbäck as the type species. The family was designated to accommodate Dothideomycete species having small ascomata and often ascostromata forming on various hosts, mostly parasitic, but also saprobic on dead plants (von Arx and Müller 1975; Hyde et al. 2013). Species in Mycosphaeriallaceae lack pseudoparaphyses and ascospores are often 2-celled, oblong to clavate, or ellipsoidal (Hyde et al. 2013). Based on these characters, the family was initially placed in the order Dothideales (von Arx and Müller 1975; Hawksworth et al. 1995; Hyde et al. 2013; Liu et al. 2015a). Kirk et al. (2001) treated the family in a separate order—Mycosphaerellales. Schoch et al. (2006) assigned Mycosphaerellaceae to Capnodiales based on phylogenetic support and this was followed by various mycologists (Crous et al. 2007, 2009; Kirk et al. 2008; Hyde et al. 2013; Wijayawardene et al. 2014a; Liu et al. 2015a). Recently, more than 50 sexual and asexual genera have been accommodated in Mycosphaerellaceae (Wijayawardene et al. 2014a). Pallidocercospora Crous et al. Pallidocercospora was introduced by Crous et al. (2013) to accommodate cercospora-like species, but not congeneric with Cercospora and is typified by P. heimii (Crous) Crous. Crous et al. (2013) designated the genus based on its pale brown cercosporoid conidia, which are generally referred to as the Mycosphaerella heimii complex (Crous et al. 2004a, 2013). Seven species were initially included in the genus based on multi-gene phylogenetic analyses (Crous et al. 2013). However, they did not synonymize Pseudocercospora colombiensis and P. thailandica under Pallidocercospora, even though these two species clustered with other Pallidocercospora species (Crous et al. 2013). Subsequently, these two species have been stated as Pallidocercospora colombiensis and P. thailandica (Crous et al. 2013; Pérez et al. 2013; Quaedvlieg et al. 2014), although, the species combinations have not been formally established. Therefore the sexual 123 20 123 Fungal Diversity (2016) 80:1–270 Fungal Diversity (2016) 80:1–270 21 morph, Mycosphaerella thailandica is transferred to Pallidocercospora in this study and this is congruent with our rDNA based phylogenies (Fig. 7). b Fig. 8 Pallidocercospora thailandica (MFLU 11-0170, MFLU 11-0177). a Herbarium material with leaf spots. b Appearance of ascomata on the host surface (a = from MFLU 11-0177, b = MFLU110170). c Close up of ascomata on the host (MFLU 11-0170). d Section through the ascomata (MFLU 11-0170). e Section through peridium (MFLU 11-0170). f–i Asci (MFLU 11-0170). j–n Ascospores (MFLU 11-0170). o–p Culture characteristics (MFLU 11-0170; o = from above, p = from below). Scale bars d = 20 lm, e = 10 lm, f–i = 5 lm, j–n = 2 lm Pallidocercospora thailandica (Crous et al.) Phookamsak, Wulandari & K.D. Hyde, comb. nov. = Mycosphaerella thailandica Crous et al., in Crous et al., Stud. Mycol. 50(2): 465 (2004) Elsinoe phaseoli CBS 150.27 Neocylindroseptoria N Ne ocylindr d oseeptoriaa pistaciae pis i taaciae CBS S 471.69 Out group Neophaeocryptopus Doth t ide d a hippophaeos hiipppoopphaaeos CBS CBS 188.58 1888.58 Dothidea Dothidea Doth t iddeea berberidis berrberridi dis C CBS BS 18 186.58 86.58 97 Dothidea Doth thiddea muelleri muuelleeri C CBS BS 191 191.58 1.58 Plowrightia Plowrigh i htia ribesia riibesia MF MFLU FLU 14-004 14-0040 40 79 Plowrightia P lowriightiaa ribesia MFLUCC MFLUCC 13-0670 Plowrightia sensu stricto Stylodothis Stylodothis Sty t loddotthhiiss puccinioides puuccinioiddes CBS S 193.58 Dothidea Dot thid idea sambuci sam mbuci DAOM DAO OM 231303 2313003 55 5 95 9 5 Dothidea Doth thide d a insculpta inscuulp l ta CBS S 189.58 Dothidea sensu stricto Dothidea Do oth t ide dea insculpta inssculp l ta MFUCC MF FUCC 13-0686 Neophaeocryptopus N eop o haeoocry ryptop o us cytisi cytis cy i i MFLUCC MFL LUC CC 14-0970 Neophaeocryptopus Dothiora Doth t iora elli elliptica ipticaa CBS 736 736.71 6.71 62 6 2 Dothiora Dothiora Do othiora bux buxi u i MFLU MFLU 15-3 15-3404 3404 Dothiora Doth t ioraa cann cannabinae nabin inae CBS 7737.71 37.71 50 Cylindroseptoria Cy C ylindr d osseepptooriaa ceratoniae cerratonniae CBS CB BS 477.69 Cylindroseptoria Coleophoma C Co leop ophomaa oleae oleaae CBS 615.72 60 0 Cylindroseptoria Coleophoma C Co leop ophomaa crateriformis cratterifo f rmi r is CBS 473.69 Endoconidioma E ndo d conniddiomaa po populi opuli U UAMH AMH H 10902 Endoconidioma Plowrightia P lowrrigh i tia periclymeni perricly l meni 178096 69 Rhizosphaera Rhi izosp s haera ka kkalkhoffii llkkhoffii o ATCC ATC CC 26605 63 6 3 Phaeocryptopus Plow wrigh i htia abietis is ATCC 24339 244339 Plowrightia Rhizosphaera Rhiz izosp s haera oudemansii oude demansii 184813 1844813 75 7 5 Phaeocryptopus Pha haeoccry ryptop o us nnudus udus CBS S 268.37 96 Rhiz i ossphaeeraa pin ni 64367 6436 367 Rhizosphaera pini Delphinella Delp l hinnellla l sstrobiligena t obilig tr igena n CB CBS BS 735.71 59 Delphinella CBS 116.29 SSydowia ydow wiaa ppolyspora oly lyspoora C BS 116 6.229 0.1 Fig. 9 RAxML maximum likelihood phylogenetic tree based on a LSU and ITS sequence data from species of Dothideaceae. Maximum likelihood bootstrap support values greater than 50 % are shown in above. The new isolates are in blue and other extype strains in bold. The tree is rooted with Elsino/ phaseoli 123 22 Fungal Diversity (2016) 80:1–270 Fig. 10 Dothiora buxi (holotype). a, b View of ascostromata on host surface. c Section through the ascostroma. d Peridium. e–g Asci. h– k Ascospores. Scale bars c = 100 lm, d = 50 lm, e–g = 30 lm, h = 20 lm, i–k = 10 lm : Pseudocercospora thailandica Crous et al., in Crous et al., Stud. Mycol. 50(2): 465 (2004) Index Fungorum number: IF552204; Facesoffungi number: FoF02258, Fig. 8 Biotrophic, hemibiotrophic, or saprotrophic on various hosts, leaf spots on the margins of leaves, causing tip 123 blight, or lesions or lesions initially start from the tip of leaves, irregular in shape, dried, pale brown to brown at the middle, and reddish-brown to dark brown at margin of the lesions. Sexual morph Ascomata 45–70 lm high, 45– 80 lm diam., as small black dots on the host surface, scattered, sometimes clustered, gregarious, immersed to Fungal Diversity (2016) 80:1–270 89 62 71 23 Hysterium hyalinum CBS 237.34 100 Hysterographium fraxini CBS 109.43 Hysterographium fraxini CBS 242.34 Rhytidhysteron opuntiae GKM 1190 Hysterodifractum partisporum CCMB 252/2012 Rhytidhysteron rufulum MFLUCC 12-0013 100 Rhytidhysteron rufulum EB 0383 Rhytidhysteron rufulum EB 0382 Rhytidhysteron rufulum EB 0384 Rhytidhysteron neorufulum HUEFS 192194 Rhytidhysteron_neorufulum GKM 361A Rhytidhysteron neorufulum MFLUCC 12-0529 Rhytidhysteron neorufulum EB 0381 80 Rhytidhysteron neorufulum MFLUCC 12-0567 Rhytidhysteron neorufulum MFLUCC 12-0011 Rhytidhysteron neorufulum MFLUCC 12-0569 Rhytidhysteron neorufulum MFLUCC 12-0528 Rhytidhysteron neorufulum CBS 306.38 72 Rhytidhysteron hysterinum EB 0351 Rhytidhysteron thailandicum MFLUCC 12-0530 99 _Gloniopsis subrugosa GKM 1214 Gloniopsis subrugosa SMH 557 Gloniopsis arciformis GKM L166A Gloniopsis praelonga CBS 123337 100 95 Gloniopsis praelonga CMW 19983 Gloniopsis praelonga CBS 112415 Gloniopsis calami MFLUCC 15–0739 Gloniopsis subrugosa GKM 1010 Gloniopsis subrugosa CBS 123346 Graphyllium caracolinensis HUEFS 42838 84 Oedohysterium sinense EB 0339 79 74 Oedohysterium insidens CBS 238.34 100 Oedohysterium insidens ANM 1443 Oedohysterium sinense CBS 123345 Hysterobrevium mori GKM 1013 Ostreichnion sassafras CBS 322.34 Hysterobrevium constrictum SMH 5211.1 97 Hysterobrevium constrictum GKM 426N Hysterobrevium smilacis CMW 18053 Hysterobrevium mori CBS 123564 Hysterobrevium mori CBS 123336 93 89 Hysterobrevium mori CBS 123563 Hysterobrevium mori CBS 123335 Hysterobrevium mori SMH 5273 96 Hysterobrevium smilacis CBS 200.34 Hysterobrevium smilacis SMH 5280 Hysterobrevium smilacis CBS 114601 Hysterobrevium mori SMH 5286 Hysterographium flexuosum GKM 1262c 78 100 Ostreichnion centramurum MFLUCC 12-0802 99 Ostreichnion curtisii CBS 198.34 Hysterium barrianum ANM 1495 100 Hysterium barrianum ANM 1442 Hysterium vermiforme GKM 1234 85 100 Hysterium pulicare ANM 1455 Hysterium pulicare ANM 85 99 Hysterium angustatum CMW 20409 70 Hysterium angustatum GKM5211 Hysterium angustatum SMH 5216 Hysterium angustatum MFLUCC 15-0950 Hysterium angustatum GKM 243A Hysterium angustatum CBS 236.34 100 Hysterium angustatum CBS 123334 Psiloglonium clavisporum CBS 123338 Psiloglonium clavisporum CBS 123341 100 Psiloglonium clavisporum CBS 123340 Psiloglonium clavisporum GKM 344A Psiloglonium clavisporum GKM L172A Psiloglonium araucanum CMW 17941 99 Psiloglonium araucanum CMW 18760 94 87 Psiloglonium araucanum CBS 112412 100 Psilogloniu simulans CBS 206.34 Psiloglonium simulans ANM 1557 Delitschia winteri CBS 225.62 0.02 Fig. 11 Phylogram generated from maximum parsimony analysis based on LSU sequence data from Hysteriaceae. Maximum likelihood bootstrap support values greater than 50 % is shown above nodes. The tree is rooted with Delitschia winteri. The new isolates are in blue and other ex-type strains are in bold semi-immersed, with protruding papilla, globose to subglobose, glabrous, ostiole central, with minute papilla. Peridium 5–10 lm wide, thin-walled, composed of 2–3 cell layers of brown to dark brown, pseudoparenchymatous cells, arranged in a textura angularis. Hamathecium lacking pseudoparaphyses. Asci (23–)25–35(–37.5) 9 7–9 lm ( x = 29 9 7.9 lm, n = 30), 8-spored, bitunicate, fissitunicate, obclavate, rarely ovoid, subsessile, apically rounded, with welldeveloped ocular chamber. Ascospores (8–)9–12 9 2.5– 3.5 lm ( x = 10.8 9 3.1 lm, n = 30), overlapping uni- to tri-seriate, clavate, hyaline to subhyaline, 1-septate, not contricted at the septum, smooth-walled, upper cell wider and shorter than lower cell. Asexual morph Hyphomycetous, pseudocercospora-like (see notes). Culture characteristics: Colonies on PDA reaching 23.5–30 mm diam. after 4 weeks at 25–30 °C; colony from above, dark greenish at the margin, paler greenish hair-like at the center; from below, dark greenish to black; dense, irregular, flattened to raised, with undulate edge, with entire margin, surface smooth, velvety to cottony; not producing pigmentation in agar. Material examined: THAILAND, Phrae, Rongkwang District, Maejo University Phrae campus grounds, on dead leaves of Dracaena loureiri Gagnep (Ruscaceae), 20 August 2010, R. Phookamsak, RP0050 (MFLU 11-0170), living culture, MFLUCC 11-0134, KUMCC; ibid. Chiang Rai, Muang District, Mae Fah Luang University campus grounds, on living leaves of Rhapis sp. (Arecaceae), 4 123 24 Fig. 12 Gloniopsis calami (holotype). a Appearance of hysterothecia on host. b Close up hysterothecia. c Section of hysteriothecium d Pseudoparaphyses. e–h. Asci i–p. Ascospores. q Geminated 123 Fungal Diversity (2016) 80:1–270 ascospore. r Culture characters on MEA. Scale bars a = 500 lm, b = 200 lm, c, e–h = 50 lm, d = 10 lm, i–q = 5 lm Fungal Diversity (2016) 80:1–270 Fig. 13 Phylogenetic tree generated from maximum parsimony (MP) analysis based on combined ITS and LSU sequence data of genera of the family Dictyosporiaceae. Bootstrap support values for maximum parsimony (MP) and maximum likelihood (ML) greater than 50 % 25 and Bayesian posterior probabilities greater than 0.80 are indicated above or below the nodes as MPBS/MLBS/PP. The ex-type strains are in bold and the new isolates are in red bold. The tree is rooted with Letendraea helminthicola 123 26 123 Fungal Diversity (2016) 80:1–270 Fungal Diversity (2016) 80:1–270 b Fig. 14 Pseudocoleophoma typhicola (holotype). a Appearance of conidiomata on host substrate. b Close up of conidioma. c Vertical section through conidiomata .d Conidia attached to conidiogenous cells. e–l Immature to mature conidia. m Germinated conidium. Scale bars a = 1 mm b = 100 lm, c = 50 lm, d–l = 5 lm, m = 10 lm August 2010, N.F. Wulandari, RP0057 (MFLU 11-0177), living culture, MFLUCC 11-0141, KUMCC. Notes: Pallidocercospora thailandica was introduced as Mycosphaerella thailandica by Crous et al. (2004b) who Fig. 15 Pseudocoleophoma typhicola (ex-type culture). a, b Culture on PDA (note b reverse). c Conidioma on PDA. d Vertical section through conidioma. e Peridium. f Conidia attached to 27 noted its asexual morph as Pseudocercospora thailandica Crous et al. (2004b). The asexual morph was described with ‘‘mycelium composed of medium brown, branched, septate, and smooth hyphae; conidiophores dense, pale brown, subcylindrical, unbranched, 0–2-septate, straight to curved, smooth-walled, arising from the upper cells of the stroma; conidiogenous cells terminal, pale brown, subcylindrical, tapering to flat tipped apical loci, proliferating sympodially; conidia solitary, pale brown, narrowly obclavate to subcylindrical, subobtuse at the apex, with conidiogenous cells. g–k Mature and immature conidia. Scale bars d = 1 mm e = 50 lm, f–k = 20 lm 123 28 Fungal Diversity (2016) 80:1–270 Fig. 16 Pseudodictyosporium thailandica (holotype). a Host (decaying bamboo). b, c Conidiophores on the host surface. d, e Conidiophores and conidia. f Conidiogenous cells and conidia. g–j Conidia. Scale bars b = 200 lm, c = 100 lm, d–e, k = 20 lm, f–j = 10 lm long obconically subtruncate at the base, 3–6-septate, smooth-walled, and guttulate’’ (Crous et al. 2004b). Based on phylogenetic analysis, Mycosphaerella thailandica clustered with Pallidocercospora species (Crous et al. 2013; Quaedvlieg et al. 2014). However, Crous et al. 123 (2013) mentioned that Pseudocercospora colombiensis and Ps. thailandica were typical members of Pseudocercospora sensu stricto based on its morphological features. Therefore, these taxa were not synonymized under the genus Pallidocercospora when Crous et al. (2013) Fungal Diversity (2016) 80:1–270 29 Stagonosporopsis chrysanthemi CBS 137.96 95/1 Stagonosporopsis chrysanthemi CBS 500.63 97/- Stagonosporopsis inoxydabilis CBS 425.90 100/1 Stagonosporopsis tanaceti CBS 131484 Stagonosporopsis rudbeckiae CBS 109180 91/0.99 Stagonosporopsis artemisiicola CBS 102636 97/1 94/0.97 97/1 Stagonosporopsis trachelii CBS 379.91 Stagonosporopsis centaureae MFLUCC 16-0787 Stagonosporopsis oculihominis CBS 634.92 66/1 100/1 Stagonosporopsis actaeae CBS 114303 Stagonosporopsis actaeae CBS 106.96 99/1 Stagonosporopsis dennisii CBS 631.68 100/1 Stagonosporopsis cucurbitacearum CBS 133.96 Stagonosporopsis Stagonosporopsis caricae CBS 248.90 Stagonosporopsis helianthi CBS 200.87 100/1 Stagonosporopsis heliopsidis CBS 109182 100/1 Stagonosporopsis andigena CBS 269.80 Stagonosporopsis andigena CBS 101.80 100/1 Stagonosporopsis crystalliniformis CBS 713.85 99/0.98 100/1 Stagonosporopsis hortensis CBS 104.42 Stagonosporopsis hortensis CBS 572.85 93/1 Stagonosporopsis astragali CBS 178.25 96/0.98 Stagonosporopsis lupini CBS 101494 52/0.96 Stagonosporopsis ajacis CBS 177.93 92/0.98 Stagonosporopsis valerianellae CBS 329.67 Stagonosporopsis dorenboschii CBS 426.90 98/1 Stagonosporopsis loticola CBS 562.81 Allophoma zantedeschiae CBS 131.93 100/1 97/- Allophoma zantedeschiae CBS 229.32 100/1 Allophoma Allophoma labilis CBS 124.93 Heterophoma sylvatica CBS 874.97 83/0.99 57/- Heterophoma nobilis CBS 507.91 84/1 Heterophoma Heterophoma adonidis CBS 114309 76/0.97 Neodidymella thailandicum MFLUCC 11-0140 100/1 Epicoccum nigrum CBS 173.73 95/1 65/0.96 Neodidymella Epicoccum nigrum CBS 125.82 Epicoccum Epicoccum pimprinum CBS 246.60 100/1 Didymella acetosellae CBS 179.97 Didymella rumicicola CBS 683.79 98/1 100/1 Didymella exigua CBS 183.55 Didymella Didymella calidophila CBS 448.83 Macroventuria anomochaeta CBS 502.72 61/- 93/1 Macroventuria anomochaeta CBS 525.71 Macroventuria Macroventuria wentii CBS 526.71 99/0.98 Paraboeremia adianticola CBS 187.83 Paraboeremia adianticola CBS 260.92 96/0.98 Paraboeremia putaminum CBS 130.69 Paraboeremia Phoma selaginellicola CBS 122.93 Fig. 17 Phylogenetic tree inferred from a maximum Likelihood analysis based on a concatenated alignment of LSU, ITS, RPB2, TUB2 sequence data representing Didymellaceae and allied families. The RAxML bootstrap support values (MLBS) greater than 50 % and Bayesian posterior probabilities (BPP) greater than 0.95 are given at the nodes (MLBS/BPP). The ex-type strains are in bold and the new isolates are in blue. The tree is rooted with Leptosphaeria doliolum introduced this genus. However, the name ‘‘Pallidocercospora thailandica’’ has been used instead of ‘‘Pseudocercospora thailandica’’ (Crous et al. 2013; Quaedvlieg et al. 2014), but the name ‘‘Pallidocercospora thailandica’’ was not formally synonymized and thus, this name was invalid. In this study, two isolates were collected from dead leaves of Dracaena loureiri (Ruscaceae) and living leaves of Rhapis sp (Arecaceae). Combined ITS and LSU phylogenetic analyses show that these isolates grouped with Pseudocercospora colombiensis and Ps. thailandica, and clustered with Pallidocercospora species, with high bootstrap support (97 % ML, 100 % MP, 1.00 PP, Fig. 7). Furthermore, Pseudocercospora colombiensis and Ps. thailandica form a distinct clade with Pseudocercospora sensu stricto which is congruent to Crous et al. (2013) and Quaedvlieg et al. (2014). Therefore, we transfer the species Mycosphaerella thailandica to the genus Pallidocercospora. Pallidocercospora thailandica is morphologically distinct from P. colombiensis, but it is difficult to distinguish these two species based on phylogenetic analyses 123 30 Fungal Diversity (2016) 80:1–270 Neomicrosphaeropsis italica MFLUCC 15–0484 Neomicrosphaeropsis italica MFLUCC 15–0485 83/1 Neomicrosphaeropsis italica MFLUCC 16–0284 Neomicrosphaeropsis italica MFLUCC 15–0487 51/- Neomicrosphaeropsis tamaricicola MFLUCC 14–0443 Neomicrosphaeropsis tamaricicola MFLUCC 14-0602 Neomicrosphaeropsis Neomicrosphaeropsis tamaricicola MFLUCC 14–0439 77/1 Neomicrosphaeropsis novorossica MFLUCC 14–0578 Neomicrosphaeropsis rossica MFLUCC 14–0586 74/0.95 87/1 Neomicrosphaeropsis minima MFLUCC 13–0394 Neomicrosphaeropsis cytisi MFLUCC 13–0396 98/1 Neomicrosphaeropsis cytisinus MFLUCC 16-0790 Ascochyta fabae CBS 649.71 77/1 Ascochyta fabae CBS 524.77 92/0.99 Ascochyta Ascochyta viciae CBS 451.68 97/1 Ascochyta pisi CBS 122785 56/0.95 99/0.98 Ascochyta pisi CBS 126.54 Phomatodes aubrietiae CBS 383.67 100/1 Phomatodes Phomatodes aubrietiae CBS 627.97 Calophoma vodakii CBS 173.53 100/1 Calophoma Coniothyrium clematidisrectae CBS 507.63 100/1 Coniothyrium clematidina CBS 108.79 75/- Phoma herbarum CBS 615.75 Phoma herbarum CBS 274.37 Phoma /Platychora Platychora ulmi MFLUCC 14–1189 53/- Platychora ulmi CBS 361.52 62/- Phoma herbarum UAMH 10909 Phoma dutchum CBS 134.96 100/1 90/- Neodidymelliopsis xanthina CBS 383.68 Neodidymelliopsis xanthina CBS 168.70 91/100/1 Neodidymelliopsis sp. 1 CBS 256.77 Neodidymelliopsis polemonii CBS 109181 98/1 Neodidymelliopsis Neodidymelliopsis ranunculi MFLUCC 13-0490 94/1 Neodidymelliopsis sp. 2 CBS 382.96 Neodidymelliopsis urticicola CBS 121.75 72/- 100/1 Xenodidymella applanata CBS 115577 Xenodidymella applanata CBS 195.36 99/1 96/1 Xenodidymella Xenodidymella catariae CBS 102635 Xenodidymella asphodeli CBS 375.62 100/0.95 Neoascochyta europaea CBS 820.84 100/1 Neoascochyta graminicola CBS 301.69 Neoascochyta Neoascochyta exitialis CBS 118.40 Leptosphaerulina australis CBS 317.83 97/100/1 Leptosphaerulina trifolii CBS 235.58 Leptosphaerulina Leptosphaerulina arachidicola CBS 275.59 Nothophoma infossa CBS 123395 100/1 Nothophoma arachidishypogaeae CBS 125.93 89/1 Nothophoma Nothophoma anigozanthi CBS 381.91 96/0.99 Microsphaeropsis olivacea CBS 432.71 Microsphaeropsis olivacea CBS 233.77 70/- Microsphaeropsidaceae Microsphaeropsis proteae CPC 1425 Leptosphaeria doliolum CBS 505.75 Leptosphaeriaceae 0.07 Fig. 17 continued (Quaedvlieg et al. 2014). However, Quaedvlieg et al. (2014) applied the pairwise homoplasy index (PHI) test with the GCPSR and CSC concepts and proposed that these two species are different taxa. Pallidocercospora thailandica has been collected from various hosts in Australia, Laos, Thailand and West Indies (Acacia mangium Willd., Eucalyptus camaldulensis Dehnh., Musa sp.) and is mostly found as a pathogen on the hosts (Crous et al. 2004b, 2013; Arzanlou et al. 2008; Cheewangkoon et al. 2008). In this study, this species was associated with leaf spots on Rhapis sp., and as a saprobe on Dracaena loureiri; both are new hosts for P. thailandica. Dothideales Lindau For Dothideales, we follow Li et al. (2016). 123 Dothideaceae Chevall. The family Dothideaceae was introduced by Chevallier (1826) as ‘Dothideae’, and later Fuckel (1869) established this family with Dothidea as the type genus and D. gibberulosa as the type species. Thambugala et al. (2014) treated the family Dothideaceae with 15 genera. Dothideaceae is characterized by ‘immersed to erumpent or superficial, uni or multi-loculate ascostromata, 8- or polyspored, bitunicate asci and hyaline or brown, transversely septate, sometimes muriform ascospores’ (Thambugala et al. 2014). We provide an updated phylogeny in Fig. 9. Dothiora buxi Jayasiri, Camporesi & K.D. Hyde, sp. nov. Index Fungorum number: IF552173; Facesoffungi number: FoF02223, Fig. 10 Fungal Diversity (2016) 80:1–270 Fig. 18 Neomicrosphaeropsis cytisi (holotype). a Herbarium specimen. b, c Appearance of brown coniodiomata on the host. d Wall of conidiomata. e Vertical section of conidiomata. f–h Conidiophores, 31 conidiogenous cells and developing conidia. j Germinating spore. i, k, l Conidia. m Culture. Scale bars b = 500 lm, c = 200 lm, d, j = 10 lm, e = 20 lm, f–h, i, k–n = 5 lm, n = 25 mm 123 32 Etymology: The specific epithet buxi is based on the host genus from which the taxon was collected. Holotype: MFLU 15-3404. Saprobic on Buxus sempervirens L. Sexual morph Ascostromata 500–1000 lm long 9 220–250 lm high, 320–340 lm diam., erumpent through the epidermis, solitary or clustered, globose, brown to black, with single locules, with a central longitudinal slit-like opening. Peridium 32–83 lm wide, two-layered, outer layer composed of dark brown or brown, thick-walled cells of textura angularis, inner layer composed of hyaline, thin-walled cells of textura angularis. Hamathecium lacking pseudoparaphyses. Asci 100–115 9 14–21 lm ( x = 102 9 17 lm, n = 20), 32-spored, bitunicate, fissitunicate, cylindro-clavate, short pedicellate, apically rounded, with a small ocular chamber. Ascospores 11–15 9 5.4–7 lm ( x = 13 9 6 lm, n = 30), bi-seriate to multi-seriate, hyaline to very pale brown, aseptate, fusoid to ovoid, one end narrower than other, smooth-walled with granular contents, with a thin mucilaginous sheath. Material examined: ITALY, Province of Forlı̀-Cesena [FC]), near Passo delle Forche—Galeata on dead branch of Buxus sempervirens (Buxaceae), 17 November 2014, E. Camporesi, IT 2284 (MFLU 15-3404, holotype, KUN, isotype). Notes: Dothiora was introduced by Fries (1849) with D. pyrenophora (Fr.) Fr. as the type species. Our isolate shares common characters with the genus Dothiora. DNA was extracted from fruiting bodies and multi-gene phylogenetic analysis placed Dothiora buxi as a sister taxon to Fig. 19 Neomicrosphaeropsis cytisinus (holotype). a Appearance of conidiomata on host. b Close-up of conidiomata. c Section of conidioma. d Section of peridium. e Conidiogenous cells. f–i Conidia. 123 Fungal Diversity (2016) 80:1–270 Fig. 20 Neomicrosphaeropsis minima (holotype). a Herbarium c specimen. b, c Appearance of black coniodiomata on the host. d, e Vertical section of conidiomata. f Wall of conidiomata. g–k Conidiophores, conidiogenous cells and developing conidia. l Germinating spore. m, n Conidia. o Culture. Scale bars b, c = 200 lm, d, e = 50 lm, f = 15 lm, g–n = 5 lm, o = 50 mm Dothiora elliptica (Fig. 9). The latter is similar in having epidermal erumpent, hysteriiforme ascostromata and hyaline ascospores but differs from Dothiora buxi in having 32-spored asci and aseptate, fusoid to ovoid, ascospores narrowed at one end (Saccardo 1889). Therefore, we introduce Dothiora buxi as a new species. Hysteriales Lindau For Hysteriales, we follow Hyde et al. (2013). Hysteriaceae Chevall. Chevallier (1826) introduced the family Hysteriaceae as ‘Hysterineae’ and this family has been treated with different genera by various authors (Zogg 1962; von Arx and Müller 1975; Kirk et al. 2001; Lumbsch and Huhndorf 2010). Recent multi-gene phylogenetic studies placed Hysteriaceae in Hysteriales, Pleosporomycetidae (Boehm et al. 2009a, b; Hyde et al. 2013; Wijayawardene et al. 2014a, Thambugala et al. 2016b). Hyde et al. (2013) and Wijayawardene et al. (2014a) accepted 13 genera, while de Almeida et al. (2014) introduced a new genus Hysterodifractum in this family. The family now contains 14 genera. A phylogenetic tree for the family is presented in Thambugala et al. (2016b) (Fig. 11). j. Germinated conidia. l Colony from above. m Colony from below. Scale bars c = 50 lm, d = 20 lm, e = 5 lm, f–i = 2 lm, j = 10 lm Fungal Diversity (2016) 80:1–270 33 123 34 Gloniopsis De Not. The genus Gloniopsis is typified by Gloniopsis praelonga (Schwein.) Underw. & Earle [as ‘praelongum’]. Based on morphology and molecular phylogenetic analyses Gloniopsis is placed in Hysteriaceae (Boehm et al. 2009b; Wijayawardene et al. 2014a; Thambugala et al. 2016b). The genus now contains 65 epithets listed in Index Fungorum (2016). The genus Gloniopsis is characterized by hyaline to yellow dictyospores, often inequilateral, curved, multi-septate, with one or more longitudinal septa, constricted at the first-formed septum, sometimes constricted at additional septa, and usually surrounded by a gelatinous sheath which may dissipate with age (Zogg 1962). Gloniopsis calami Konta & K.D. Hyde. sp. nov. Index Fungorum number: IF552234; Facesoffungi number: FoF02366, Fig. 12 Etymology: Name reflects the host genus Calamus. Holotype: MFLU 15-1470. Saprobic on dead Calamus sp. Sexual morph Hysterothecia 195–215 lm high 9 160–170 lm wide, erumpent to superficial, solitary to gregarious, scattered, dark, straight to flexuous. Peridium 37–47 lm wide, carbonaceous, thickwalled, not having distinct layers, relatively smooth on the outer surface. Hamathecium 1.2–2 lm wide, composed of dense, branched, hyaline, septate, pseudoparaphyses. Asci 60– 80 9 15–21 lm ( x = 71 9 17 lm, n = 10), 8-spored, bitunicate, fissitunicate, cylindrical to cylindric-clavate, short pedicellate, with knob-like pedicel, apically rounded, with a well-developed ocular chamber, external layer easily broken. Ascospores 17–20 9 6–8 lm ( x = 19 9 7 lm, n = 10), dictyosporous, overlapping 1–2-seriate, fusiform, slightly curved to straight, 4–6-trans-septate and with 2–4 vertical septa, reddish-brown to brown, constricted at the septa, smooth-walled. Asexual morph Undetermined. Culture characteristics: Ascospores germinated on MEA within 24 h and germ tubes produced from all cells. Colonies on MEA 7–7.5 cm diam. after 2 weeks at 25 °C, grey to dark green, outwardly with strongly radiating colony. After 1 month of incubation, colonies irregular, convex, spongy, medium dense, margin undulate. Material examined: THAILAND, Phang-Nga Province, on dead Calamus sp. (Arecaceae), 6 December 2014, S. Konta, DNH07i (MFLU 15-1470, holotype, isotype HKAS 95030); ex-type living culture, MFLUCC 15-0739. Notes: Molecular analyses indicate that the new species belongs to the genus Gloniopsis in the Hysteriaceae clade and is nested in between G. praelonga (type) and G. subrugosa and appear to be phylogenetically distinct (Fig. 11). The ascospores of G. calami are similar in shape, but smaller in size and have a different septation to G. praelonga (type) and G. subrugosa. However, G. calami is distinct from G. arciformis in its ascospores being 123 Fungal Diversity (2016) 80:1–270 constricted at the septa, while in G. arciformis they are not constricted. Pleosporales Luttr. ex M.E. Barr We follow Tanaka et al. (2015). Dictyosporiaceae Boonmee & K.D. Hyde The family Dictyosporiaceae was introduced by Boonmee et al. (2016) to accommodate Aquaticheirospora, Cheirosporium, Dictyocheirospora, Dictyopalmispora, Dictyosporium, Digitodesmium, Pseudocoleophoma and Pseudodictyosporium. The asexual morphs of the family Dictyosporiaceae are hyphomycetous with brown, multiseptate, cheirosporous conidia (Boonmee et al. 2016). In this study we provide an updated backbone tree for Dictyosporiaceae (Fig. 13) and introduce the new species Pseudodictyosporium thailandica and Pseudocoleophoma typhicola. Pseudocoleophoma typhicola E.B.G. Jones, Kamolhan, Boonmee & K.D. Hyde, sp. nov. Index Fungorum number: IF552326; Facesoffungi number: FoF02444, Figs. 14 and 15 Etymology: Referring to the host plant Typha latifolia. Holotype: MFLU 16-0966. Saprobic on submerged stems in freshwater. Sexual morph Undetermined. Asexual morph Conidiomata forming as dark spots on the host surface, 140–150 lm high 9 60–100 lm diam. ( x = 143 9 80 lm, n = 15), semi-erumpent in the host tissue, uniloculate solitary to scattered, subglobose, brown to black. Peridium 40–45 lm at base, 40–45 lm at sides, comprising 4–5 layers, hyaline to dark brown, thick-walled cells of textura angularis. Conidiophores reduced to conidiogenous cells. Conidiogenous cells 2–5 9 2–5 lm ( x = 2 9 3 lm, n = 15), enteroblastic, smooth-walled, hyaline. Conidia 9–11 9 2– 3 lm ( x = 10 9 3 lm, n = 20), hyaline, oblong to cylindrical, with rounded or obtuse ends, 1-euseptate, smooth, thin-walled, guttulate. Culture characteristics: Colonies on PDA 3 cm diam. after 4 weeks at 16 °C, dirty white to pale brown at the margin, pale brown to creamy at the center; reverse iron, thin, curled, flat. Material examined: UK, Hampshire, Swanick Lakes, on submerged stems of Typha latifolia (Typhaceae) in freshwater, 28 August 2015, E.B.G. Jones, GJ190 (MFLU 16–0966, holotype, HKAS94520 isotype), ex-type living culture, MFLUCC 16–0123, KUMCC 16-0007. Notes: Pseudocoleophoma typhicola is different from P. polygonicola and P. calamagrostidis in having conidiomata without neck and with a wide peridium (40–45 lm at base, 40–45 lm at sides), enteroblastic, 3-septate conidia while P. polygonicola and P. calamagrostidis have Fungal Diversity (2016) 80:1–270 35 Fig. 21 Neodidymelliopsis ranunculi (holotype). a Herbarium specimen. b, c Appearance of black coniodiomata on the host. d Vertical section of conidioma. e, f Vertical section of peridium. g–j Conidiogenous cells and developing conidia. k Germinating conidium. l– n Conidia. o Culture on PDA. Scale bars b = 200 lm, c– d = 100 lm, e = 50 lm, f = 20 lm, g–i = 10 lm. k–p = 5 lm, q, r = 20 mm conidiomata with a long neck, phialidic, aseptate conidia. Phylogenetic analyses indicate that Pseudocoleophoma typhicola is related to P. polygonicola and P. calamagrostidis with high support, but it can be recognized as a new species as it stands on its own with relatively good support (Fig. 13). 123 36 123 Fungal Diversity (2016) 80:1–270 Fungal Diversity (2016) 80:1–270 b Fig. 22 Platychora ulmi (MFLU 16-1972). a, b Ascostromata on substrate. c, d Cross section of ascostromata. e Peridium f, i. Asci j– l. Ascospores. m Conidiomata on MEA. o Cross section of conidiomata. n, p, q Conidia attached to possible conidiogenous cell. r Conidia. Scale bars a = 500 lm, b, c, m, o = 100 lm, d = 50 lm, e = 20 lm, f–i = 10 lm, j–l, n, p–r = 5 lm Pseudodictyosporium Matsush. The genus Pseudodictyosporium was established by Kobayasi (1971) and is typified by P. wauense Matsush. Three species are accepted in this genus, P. wauense, P. elegans and P. indicum (Boonmee et al. 2016). Molecular phylogenetic analyses place Pseudodictyosporium within the family Dictyosporiaceae (Pleosporales) (Tanaka et al. 2015; Boonmee et al. 2016). In this study we introduce P. thailandica as a new species. Pseudodictyosporium thailandica C.G. Lin, Yong Wang bis & K.D. Hyde, sp. nov. Index Fungorum number IF552165; Facesoffungi number: FoF02227, Fig. 16 Etymology: Referring to the country where the fungus was first collected. Holotype: MFLU 16-1301. Saprobic on decaying bamboo stem. Sexual morph Undetermined. Asexual morph Conidiophores macronematous, mononematous, scattered or caespitose, erect, flexuous, irregularly branched, smooth, septate, slightly constricted at septa, hyaline to brown, often geniculate, 23– 305 lm long ( x = 77 lm, n = 27), 2.5–7.9 lm wide ( x = 5 lm, n = 96). Conidiogenous cells holoblastic, polyblastic, discrete, determinate or sympodial, terminal and intercalary. Conidia solitary, acropleurogenous, dry, cheiroid, ellipsoidal, ovoid, smooth, multi-septate, subhyaline to grey-brown, 14–31 lm long ( x = 20 lm, n = 70), 12–26.5 lm wide ( x = 17 lm, n = 70) at the widest point. Culture characteristics: Conidia germinating on PDA within 36 h. Colonies on MEA reaching 20–35 mm diam. after 4 months at room temperature (25 °C), effuse, hairy, grey-white from above, brown at the center, yellowishwhite at margin from below. Material examined: THAILAND, Phetchaburi, Cha-am District, on decaying bamboo stem, 28 July 2015, ChuanGen Lin, KNP 5-3 (MFLU 16-1301, holotype; HKAS 95053, isotype), ex-type living culture, MFLUCC 16-0029. Notes: Phylogenetic analysis of combined ITS, LSU, SSU and TEF sequence data indicate that our species belongs in the genus Pseudodictyosporium (Fig. 13) with 100 % MP bootstrap support, 100 % ML bootstrap support and 100 % Bayesian posterior probabilities, and forms a separate clade within Pseudodictyosporium. 37 The conidiophores of P. thailandica are longer than those of P. wauense (up to 100 lm) and P. elegans (10–56 lm), but shorter than those of P. indicum (284–630 lm). In addition, the conidia of our species (12–26.5 lm) are wider than earlier described species (P. elegans 9–16.5 lm, P. indicum 12.5–16 lm and P. wauense 12–19 lm) (Rao and Subhedar 1976; Tzean and Chen 1990; Kirschner et al. 2013). Didymellaceae Gruyter et al. The family Didymellaceae was introduced by De Gruyter et al. (2009), with type species Didymella exigua (Niessl) Sacc., to accommodate most species in Phoma sensu lato and allied genera. The family contains numerous plants pathogenic, saprobic and endophytic species associated with a wide range of hosts (Aveskamp et al. 2010; Chen et al. 2015a). Aveskamp et al. (2010) revised the taxonomy of Didymellaceae based on multi-gene analyses and included eleven genera in the family, i.e. Ascochyta, Boeremia, Chaetasbolisia, Didymella, Epicoccum, Leptosphaerulina, Macroventuria, Microsphaeropsis, Peyronellaea, Phoma and Stagonosporopsis. Subsequently, more genera and information were added (Wijayawardene et al. 2012; Zhang et al. 2012a; Hyde et al. 2013; Ariyawansa et al. 2015a). Chen et al. (2015a) utilized the RPB2 gene combined with ITS, LSU as well as tub2 to distinguish Phoma and related genera and accepted 17 genera in Didymellaceae. However Microsphaeropsis was excluded from the Didymellaceae, and a new family Microsphaeropsidaceae was proposed to accommodate these Microsphaeropsis species. Thambugala et al. (2016a) included an additional genus Neomicrosphaeropsis due to its morphological similarity with Microsphaeropsis species, but this is phylogenetically closely related to Didymellaceae. In this study, three new species are added in Neomicrosphaeropsis based on both morphology and phylogeny. Moreover, a new collection of Platychora ulmi (J. Schröt.) Petr. MFLUCC 14-1189 together with another strain CBS 361.52 from GenBank clustered with the Phoma group (Fig. 17), but LSU gene data are only available for these two strains. LSU and SSU sequence data does not provide sufficient phylogenetic information to distinguish closely related genera or species (Aveskamp et al. 2009, 2010; Chen et al. 2015a). Thus, the genus Platychora should be re-evaluated based on additional genes. Didymellocamarasporium was introduced in Didymellaceae based on LSU and SSU sequence data by Wijayawardene et al. (2016), but they cannot be well separated from other genera in present study (data not shown). Hence, this genus also needs additional genes to confirm its placement in Didymellaceae. To date, 21 genera are included in Didymellaceae. 123 38 Fungal Diversity (2016) 80:1–270 Fig. 23 Stagonosporopsis centaureae (holotype). a Appearance of ascomata on host. b Close-up of ascoma. c Section of ascoma. d Section of peridium. e Pseudoparaphyses. f–i Asci. j–l Ascospores. m Germinated ascospore. n Colony from above. o Colony from below. Scale bars c = 20 lm, d, e = 10 lm, f–i = 20 lm, j– m = 5 lm Neomicrosphaeropsis Thambugala et al. Thambugala et al. (2016a) included Neomicrosphaeropsis in Didymellaceae. In this study, three new species are added in Neomicrosphaeropsis based on both morphology and phylogeny. Etymology: Named after the host genus Cytisus. Holotype: MFLU 16-1871 Saprobic on dead stem of Cytisus sp. (Fabaceae), forming numerous, conspicuous, oval, dark brown, conidiomata. Sexual morph Undetermined. Asexual morph Coelomycetous. Conidiomata 75–155 lm diam. 9 75– 130 lm high, dark brown, solitary to gregarious or confluent, pycnidial, globose to subglobose, immersed, unilocular, thick-walled, smooth, ostiolate. Ostiole single, short, with acute apex, centrally located. Wall of Neomicrosphaeropsis cytisi W.J. Li, Camporesi & K.D. Hyde, sp. nov. Index Fungorum number: IF552212; Facesoffungi number: FoF02347, Fig. 18 123 Fungal Diversity (2016) 80:1–270 conidiomata 10–24 lm wide, composed of thick-walled, brown to hyaline cells of textura angularis. Conidiophores reduced to conidiogenous cells. Conidiogenous cells 2– 4 lm long 9 3.5–6 lm wide, hyaline, enteroblastic, phialidic, doliiform to ampulliform, determinate, discrete, glabrous. Conidia 4.5–7 9 3–5 lm ( x = 5.7 9 3.9, n = 30), initially hyaline, becoming pale brown to dark brown at maturity, globose to obovate, ellipsoidal to subcylindrical, rounded at both ends, unicellular, thick-walled, smooth. Culture characteristics: Colonies on PDA attaining 30–40 mm diam. after 4 weeks at 20–25 °C, with circular margin, dark jacinth to orange red to dark olivaceous, flattened, dense, aerial mycelium on the surface, reverse similar in colour. Material examined: ITALY, Province of Arezzo [AR], Bagno di Cetica, on dead stem of Cytisus sp. (Fabaceae), 7 October 2012, Erio Camporesi, IT-784 (MFLU 16-1871, holotype); ex-type living culture, MFLUCC 13-0396, ICMP; ibid. IT-784B (HKAS 93585, isotype); living culture, KUMCC 16-0026. Notes: Neomicrosphaeropsis cytisi differs from N. cytisinus in the form of the conidiomata. Neomicrosphaeropsis cytisi has immersed, ostiolate conidiomata that are smaller than those of N. cytisinus which are semiimmersed when immature, and become erumpent at maturity (190–220 lm high 9 210–250 lm diam.) Neomicrosphaeropsis cytisinus Tennakoon, Camporesi & K.D. Hyde, sp. nov. Index Fungorum number: IF552262; Facesoffungi number: FoF02396, Fig. 19 Etymology: Name reflects the host genus Centaurea, from which the holotype was collected. Holotype: MFLU 16-1364. Saprobic on Cytisus sp. Sexual morph Undetermined. Asexual morph Coelomycetous Conidiomata 190–220 lm high 9 210–250 lm diam. ( x = 232 9 204, n = 10), stromatic, solitary, immersed to semi-immersed when immature, becoming erumpent at maturity, globose to subglobose, uniloculate, black, dehiscing by an irregular split of the host epidermis. Peridium 20–25 lm wide, composed of 4–5 layers of light brown cells of textura angularis to textura prismatica. Conidiogenous cells 1– 2 lm wide, phialidic, hyaline, thin-walled, smooth, integrated, producing a single conidium at the apex. Conidia 5–7 9 3–5 lm ( x = 67 9 4.2, n = 30), initially hyaline, becoming light brown, moderately thick-walled, smooth, aseptate, ovoid, obtuse at apex, truncate or rounded at base. Culture characteristics: Colonies on PDA reaching 25–30 mm diam. after 8 days at 20–25 °C, medium sparse, circular, flat, slightly rough at surface with entire edge, with a well-defined margin, cottony to fairly fluffy with 39 sparse mycelium; from above: white to cream at the margin, white to yellowish at the centre; from below, light yellow to light brown at the margin, yellowish at the centre; mycelium white to cream with tufting; not producing pigments in PDA medium. Material examined: ITALY, Province of Arezzo [AR], near Croce di Pratomagno, on dead stem of branch of Cytisus scoparius L. (Fabaceae), 24 June 2012, E. Camporesi, IT 472 (MFLU 16-1364, holotype; HKAS 93703, isotype), ex-type living cultures, MFLUCC 16-0790, KUMCC 15-0557. Notes: Neomicrosphaeropsis cytisinus resembles N. cytisi in sharing the size range of conidiophores and conidiogenous cells, but differs in the size of conidiomata (75–130 9 74–157 lm) and thickness of peridium (10–24 lm). Neomicrosphaeropsis cytisinus differs from Neomicrosphaeropsis minima in the size of conidiomata (3–5.5 9 2–4 lm), conidiophores and host. Phylogenetic analyses show that they are distinct with high bootstrap support (98 % ML, 1.00 BYPP, Fig. 17). Neomicrosphaeropsis minima W.J. Li, Camporesi & K.D. Hyde, sp. nov. Index Fungorum number: IF552213; Facesoffungi number: FoF02348, Fig. 20 Etymology: Named for the small conidiomata. Holotype: MFLU 16-1490 Saprobic on dead stems of Verbascum sp. (Scrophulariaceae), forming numerous, conspicuous, rounded, black conidiomata. Sexual morph Undetermined. Asexual morph Coelomycetous. Conidiomata 60–80 lm diam., 60–95 lm high, black, solitary to gregarious or confluent, pycnidial, globose to subglobose, immersed or semi-immersed, unilocular, thick-walled, smooth, ostiolate. Ostiole single, short, centrally located. Wall of conidiomata 8– 15 lm wide, composed of thick-walled, brown to hyaline cells of textura angularis. Conidiophores 2.7–5.5 lm long 9 3.2–5.5 lm wide, occasionally present, hyaline, doliiform to ampulliform, arising from inner layers of the pycnidial wall. Conidiogenous cells 2.6–5.5 lm long 9 2– 3.5 lm wide, hyaline, enteroblastic, phialidic, doliiform or cylindrical to ampulliform, with a periclinal wall thickening at the tip, smooth. Conidia 2.8–5.4 9 2–3.6 lm ( x = 4.1 9 2.8, n = 30), hyaline when young, becoming brown at maturity, oval, rounded at both ends, unicellular, thick-walled, smooth, guttulate. Culture characteristics: Colonies on PDA attaining 15–20 mm diam. after 7d at 20–25 °C with circular margins, white to dark brown, flattened, with felt-like, dense, aerial mycelium on the surface, reverse with dark brown in the central zone, white on the edge, sporulating. Material examined: ITALY, Province of Arezzo [AR], near Montemignaio, on dead stems of of Verbascum sp. 123 40 123 Fungal Diversity (2016) 80:1–270 Fungal Diversity (2016) 80:1–270 b Fig. 24 RAxML tree based on a combined dataset of LSU, SSU, ITS and TEF partial sequences of Didymosphaeriaceae. Bootstrap support values for maximum likelihood (ML) higher than 60 % and Bayesian posterior probabilities (BYPP) greater than 0.90 are defined as above the nodes respectively. The tree is rooted to Pleospora herbarum. The ex-type strains are in bold; the new isolates are in blue (Scrophulariaceae), 1 October 2012, Erio Camporesi, IT765 (MFLU 16-1490, holotype); ex-type living culture, MFLUCC 13-0394; ibid. IT-765B (HKAS 95027, isotype); living culture, KUMCC 16-0024. Notes: Phylogenetic tree based on multi-gene (LSU, ITS, RPB2 and b-tubulin) shows that the three collections (MFLUCC 13-0394, MFLUCC 13-0396, KUMCC 15-0557) cluster with newly introduced genus Neomicrosphaeropsis (Thambugala et al. 2016a). However, these strains formed a separate branch, basal to N. rossica Thambugala, Bulgakov & K.D. Hyde. Morphologically, they share similar conidia characters with the type species N. italica. Neomicrosphaeropsis minima is closely related to N. cytisi in our phylogenetic tree (Fig. 17), but can be easily distinguished by the dimensions of the conidiomata. Neomicrosphaeropsis minima has immersed to semi-immersed conidiomata smaller than these of N. cytisi which has conidiomata 75–155 lm diam. 9 75–130 lm high. In addition, N. minima has conidiogenous cell walls, thickened at the apex, while this character is not observed in N. cytisi. Neodidymelliopsis Q. Chen & L. Cai Neodidymelliopsis was introduced to accommodate Neodidymelliopsis cannabis (G. Winter) Q. Chen & L. Cai. as the type species, N. polemonii (Cooke) Q. Chen & L. Cai, N. xanthine (Sacc.) Q. Chen & L. Cai, and two unidentified species (Chen et al. 2015b). The conidia of this genus are variable in shape: ovoid to ellipsoidal, cylindrical, allantoid, hyaline to pale brown or pale yellowish, 0–1septate. Neodidymelliopsis ranunculi W.J. Li, Camporesi & K.D. Hyde, sp. nov. Index Fungorum number: IF552214; Facesoffungi number: FoF02349, Fig. 21 Etymology: Named after host genus Ranunculus. Holotype: MFLU 16-1870 Saprobic on dead stem of Ranunculus sp. (Ranunculaceae), Sexual morph undetermined. Asexual morph Coelomycetes. Conidiomata 100–120 lm high, 95– 110 lm diam., pycnidial, solitary, globose to subglobose, black, immersed to semi-immersed, unilocular, ostiolate, wall 14–32 lm wide, composed of dark brown to light brown, thick-walled cells of textura angularis. Ostiole single, short, eccentric. Conidiophores reduced to conidiogenous cells. Conidiogenous cells 3–5 lm long 9 4.5– 41 10 lm wide, hyaline, enteroblastic, phialidic, determinate, discrete, doliiform, with a small collarette, smooth, arising from inner layers of conidiomata. Conidia 3–5 9 7.5– 10 lm ( x = 4.3 9 8.7 lm, n = 30), initially hyaline, becoming pale brown at maturity, ellipsoidal, rounded at both ends or 1-septate, straight or slightly curved, guttulate, smooth-walled. Culture characteristics: Colonies on PDA attaining 40–50 mm diam. after 7d at 20–25 °C, margins circular, floccose, white, pale grey to olivaceous near the center; reverse with white margin, dark brown at central zone, sporulating after 4 weeks. Material examined: ITALY, Province of Forlı̀-Cesena [FC], Castrocaro Terme e Terra del Sole, near Converselle, on dead stem of Ranunculus sp. (Ranunculaceae), 2 December 2012, Erio Camporesi, IT-936 (MFLU 16-1870, holotype); ex-type living culture, MFLUCC 13–0490, ICMP; ibid. (KUN, HKAS 95028, isotype), living culture, KUMCC 16-0025. Notes: Neodidymelliopsis ranunculi fits well within the morphological concept for Neodidymelliopsis. Based on both morphology and phylogeny, N. ranunculi is introduced as a new species in the genus. Platychora Petr. The genus Platychora is typified by P. ulmi (J. Schröt.) Petr. and comprises the type species and P. alni (Peck) Petr. (Index Fungorum 2016). This genus is characterized by multi-loculate stromatic tissues and it produces apiospores. Platychora ulmi (J. Schröt.) Petr., Annls mycol. 23(1/2): 103 (1925) = Sphaeria ulmi Schleich. ex Fr., Observ. mycol. (Havniae): 173 (1815) Index Fungorum number: IF280985; Facesoffungi number: FoF02431, Fig. 22 Reference specimen: MFLU 16-1972 Saprobic on dead leaves of Ulmus sp. Sexual morph Stromata up to 1 mm broad, sub-epidermal, solitary to aggregated, superficial, cushion-shaped, multi-loculate, black. Locules 100–130 lm high 9 120–170 lm diam. ( x = 216 9 250 lm, n = 10), immersed in stromatic tissues, globose to subglobose, coriaceous, papillate, ostiolate. Peridium 13–16 lm wide ( x = 15 lm, n = 10), comprising thick-walled, dark brown cells of textura angularis. Hamathecium comprising 3.5–5.5 lm wide ( x = 5 lm, n = 10), filiform, hyaline, branched pseudoparaphyses. Asci 50–100 9 8.5–10.5 lm ( x = 76 9 10 lm, n = 10), 8spored, bitunicate, cylindrical, short pedicellate. Ascospores 10–15 9 3–6 lm ( x = 13 9 5 lm, n = 10), obliquely uniseriate, apiosporous, hyaline, ovoid, with a transverse septum near the lower end, not constricted at the septum. 123 42 Fig. 25 Montagnula cirsii (holotype). a Herbarium material. b, c Ascomata semi-immersed in the stem. d Vertical hand section of ascoma. e Ostiole. f Vertical hand section of peridium. g Immature asci. h, i, k Asci with ascospores. Note the bitunicate asci. 123 Fungal Diversity (2016) 80:1–270 j Pseudoparaphyses. n Germinating ascospore. o–s Ascospores. l Colony on PDA from above. m Colony on PDA from below. Scale bars b = 500 lm, c = 100 lm, d = 50 lm, e–f, n = 20 lm, g– j = 10 lm, k, o–s = 5 lm Fungal Diversity (2016) 80:1–270 Asexual morph Conidiomata 130–140 lm high 9 190– 210 lm diam. ( x = 138 9 200 lm, n = 10), subcuticular, acervular, brown to black, applanate to pulvinate. Conidiophores reduced to conidiogenous cells. Conidiogenous cells 10–15 lm high 9 2–4 lm wide ( x = 138 9 200 lm, n = 10), solitary, septate at the base, olivaceous, smooth, cylindrical, straight, holoblastic, annellidic. Conidia 3– 5.5 9 2.5–3 lm ( x = 5 9 2.7 lm, n = 10), oval, pale brown, aseptate, thin-walled, smooth, ornamented. Culture characteristics: Colonies growing on MEA, becoming 1 cm within 7 days at 18 °C, circular, flat, irregular margin, with sparse aerial mycelium, white. Material examined: ITALY, Province of Forlı̀-Cesena [FC], Isola di Santa Sofia, on dead leaves of Ulmus sp. (Ulmaceae), 20 January 2014, Erio Camporesi, IT 1670 (MFLU 16-1972, reference specimen designated here), living culture, MFLUCC 14-1186. Notes: The phylogenetic placement of Platychora ulmi is not clear. Most phylogenetic studies placed this genus in Pleosporales without assignment to any family (Winton et al. 2007). However Hyde et al. (2013) included Platychora within Didymellaceae and combined ITS, LSU, RPB2 and Beta tubulin gene analysis in this study (Fig. 17) support the taxonomic placement of Platychora ulmi in Didymellaceae. We also illustrate the asexual morph of Platychora ulmi from culture. Stagonosporopsis Died. Stagonosporopsis was introduced by Diedicke (1912). This genus is characterised by having conidia with one, two or occasionally three septa. Jaczewski (1917) treated Stagonosporopsis as a subgenus of Ascochyta, whereas Petrak (1925) reduced this genus to synonymy with Ascochyta. Stagonosporopsis centaureae Tennakoon, Camporesi & K.D. Hyde, sp. nov. Index Fungorum number: IF552261; Facesoffungi number: FoF02397, Fig. 23 Etymology: Name reflects the host genus Centaurea, from which the species was collected. Holotype: MFLU 16-1365 Saprobic on dead stem of Centaurea cyanus. Sexual morph Ascomata 110–120 lm high 120–130 lm diam., solitary, scattered, partly immersed to superficial, raised, dark brown to black, globose to subglobose, glabrous, uniloculate, with centrally located ostiole with minute papilla. Peridium 22–27 lm wide, thin-walled, with unequal thickness, composed of 4–5 layers of dark brown to black pseudoparenchymatous cells, arranged in textura angularis to textura prismatica. Hamathecium composed of numerous, 2–2.5 lm wide, filamentous, distinctly septate, cellular pseudoparaphyses, non-constricted at the septum, 43 anastomosing at the apex, embedded in a hyaline gelatinous matrix. Asci 35–48 9 7.2–8.2 lm ( x = 42.5 9 8.1 lm, n = 30), 8-spored, bitunicate, fissitunicate, cylindrical or clavate, short pedicellate, with furcate pedicel, apically rounded with an indistinct ocular chamber. Ascospores 13– 15 9 3–5 lm ( x = 14.3 9 4.3 lm, n = 30), overlapping, uni- to bi-seriate, hyaline, ellipsoidal to clavate or fusiform with rounded ends, 1-septate, slightly constricted at the septum, echinulate, straight to slightly curved. Asexual morph Undetermined. Culture characteristics: Colonies on PDA reaching 25–30 mm diam. after 8 days at 20–25 °C, colonies medium sparse, circular, flat, slightly rough surface with entire edge, well defined margin, cottony to fairly fluffy with sparse mycelium; from above: dark brown to yellowish at the margin, dark brown to black at the centre; from below: dark brown to yellowish-brown at the margin, dark brown at the centre; mycelium light brown to yellowish with tufting; not producing pigments in PDA medium. Material examined: ITALY, Province of Arezzo [AR], near Quota, on dead stem of Centaurea cyanus (Asteraceae), 24 June 2015, E. Camporesi, IT 2548 (MFLU 16-1365 holotype; HKAS 93705 isotype), ex-type living cultures, MFLUCC 16-0787, KUMCC 15-0559. Notes: Stagonosporopsis centaureae clusters with Stagonosporopsis trachelii with high support (93 % ML, 0.99 BYPP, Fig. 17). It can be easily distinguished from S. trachelii which has smaller ascomata (60–80 lm diam.) and smaller ascospores (4–6 9 1.5–2.5 lm). Didymosphaeriaceae Munk Details of this family can be seen in Ariyawansa et al. (2014a), Li et al. (2016) and Wanasinghe et al. (2016). Montagnula Berl. The genus Montagnula was introduced by Berlese (1896) to accommodate M. infernalis (Niessl) Berl. and M. gigantea (Mont.) Berl. Ariyawansa et al. (2014c) placed Montagnula in the family Didymosphaeriaceae including some phragmosporous and didymosporous species, based on morphology and available phylogenetic analysis. Montagnula was accepted as a distinct genus in the family Didymosphaeriaceae in several recent studies (Ariyawansa et al. 2014c; Hongsanan et al. 2015a; Li et al. 2016). Presently, there are 28 species in this genus including two recently described species, M. bellevaliae Wanasinghe et al. and M. scabiosae Wanasinghe et al. (Hongsanan et al. 2015a). Montagnula cirsii Qing Tian, Camporesi & K.D. Hyde, sp. nov. Index Fungorum number: IF552184; Facesoffungi number: FoF02255, Fig. 25 123 44 123 Fungal Diversity (2016) 80:1–270 Fungal Diversity (2016) 80:1–270 b Fig. 26 Tremateia arundicola (holotype). a Appearance of asco- mata on host substrate. b Section of ascoma. c Peridium. d Pseudoparaphyses. e–g Asci. h–k Ascospores. Scale bars b, c = 50 lm, d = 10 lm, e–g = 20 lm, h–k = 10 lm Etymology: In reference to the host genus Cirsium, from which this holotype was collected. Holotype: MFLU 14-0730. Saprobic under periderm or semi-immersed in woody plant substrates. Sexual morph Ascomata 385–415 lm diam. 9 510–525 lm high ( x = 400 9 515.5 lm, n = 10), semi-immersed to erumpent, solitary, scattered, or sometimes gregarious, globose, black, smooth-walled, neck long, with a small, flattened, ellipsoid ostiole at the apex. Peridium 41–58.5 lm ( x = 43.3 lm, n = 6), 2-layered, the outer layer composed of irregular, thick-walled, brown cells of textura angularis; the inner layer composed of hyaline, smaller cells of textura angularis. Hamathecium comprising 1–2 lm broad, septate, long, colourless, branched or simple pseudoparaphyses, surrounding the asci. Asci 84.5–119.5 9 10.5–13.5 lm ( x = 101 9 12 lm, n = 10), 8-spored, bitunicate, clavate, long pedicellate, apically rounded with an ocular chamber. Ascospores 18– 23.5 9 6.5–9.5 lm ( x = 21.5 9 8 lm, n = 10), overlapping uni-seriate or uni-seriate, 3-septate, ellipsoid to fusiform, curved, yellow to brown, constricted at the septa, broader at the middle two cells, tapering or obtuse at both ends, smooth-walled. Asexual morph Undetermined. Culture characteristics: Ascospore germinating on PDA within 12 h. Colonies on PDA, reaching 8 mm diam. in 7 days at 25 °C. Mycelium superficial, hyaline, hairy, with entire edge, floccose at the center, drift white from above and light brown at the center from below. Material examined: ITALY, Province of Forlı̀-Cesena [FC], Balze-Verghereto, on dead stem of Cirsium sp. (Compositae), 21 May 2013, Erio Camporesi (MFLU 14-0730, holotype); ibid., (HKAS 94523, isotype); ex-type living culture, MFLUCC 13-0680, KUMCC 16-0018. Notes: Montagnula cirsii is morphologically similar to M. bellevaliae and M. scabiosae (Hongsanan et al. 2015a). However, they can be distinguished by various characters, such as host, shape of ascomata and different sizes of asci. Montagnula bellevaliae occurs on dead stems of Bellevalia romana, M. scabiosae on dead stems of Scabiosa sp. and M. cirsii on dead stems of Cirsium sp. The orientation of ascomata is also different. Montagnula bellevaliae has an eccentric papilla, while the other two species have regular papilla. The size of asci is also quite different; Montagnula cirsii is smaller than M. scabiosae (84.5–119.5 9 10.5–13.5 lm vs. 110–130 9 14–20 lm) but larger than M. bellevaliae which is 70–100 lm in length, 9–12 lm in width.). Based on phylogenetic analysis (Fig. 24), our strain clusters with M. scabiosae in Montagnula with 45 relatively high support (99 % MP /1.00 PP). We therefore introduce a new species based both on morphology and phylogeny. Tremateia Kohlm. Facesoffungi number: FoF00223 Tremateia was introduced as a facultative marine genus, characterized by ‘depressed globose, immersed ascomata, numerous cellular pseudoparaphyses, fissitunicate and clavate asci, ellipsoid muriform ascospores, and a Phomalike asexual morph’ (Kohlmeyer et al. 1995; Ariyawansa et al. 2014a). Earlier the genus was identified as similar to Lewia and Diademosa and placed in Pleosporaceae (Kohlmeyer et al. 1995). DNA sequences based studies have revealed that T. halophila groups in Didymosphaeriaceae ( x = Montagnulaceae), sister to Bimuria novae-zelandiae (Schoch et al. 2009; Suetrong et al. 2009; Ariyawansa et al. 2014a, 2015a, b, c; Liu et al. 2015a; this study). Ariyawansa et al. (2014a) confirmed the familial status of Tremateia in Didymosphaeriaceae. In this study the novel isolates of Tremateia arundicola and T. guiyangensis grouped with T. halophila sister to Bimuria novae-zelandiae. However these Tremateia members are separated from Bimuria novae-zelandiae with high statistical support in combined (LSU, SSU, ITS and TEF) gene analyses (98 % ML and 1.00 BYPP, Fig. 24) Tremateia arundicola Wanasinghe, E.B.G. Jones & K.D. Hyde, sp. nov. Index Fungorum number: IF552134; Facesoffungi number: FoF02210, Fig. 26 Etymology: Name reflects the place Arun River, from which the species was collected. Holotype: MFLU 16-1275 Saprobic on dead herbaceous stems. Sexual morph Ascomata 200–300 lm high 250–350 lm diam. ( x= 259.3 9 295.1 lm, n = 5) immersed to semi-erumpent, solitary, scattered, broadly oblong to sub globose and flattened, dark brown to black, coriaceous, ostiolate. Ostiole 40–60 lm high 50–70 lm diam. papillate, black, smooth, filled with hyaline cells. Peridium 10–20 lm wide at the base, 15–25 lm wide in sides, thick, with 4–5 layers, outer layer heavily pigmented, thick-walled, comprising reddish to dark brown cells of textura angularis, inner layer composed of hyaline thin-walled cells of textura angularis. Hamathecium comprising numerous, 3–4 lm wide, filamentous, branched, septate, pseudoparaphyses. Asci 170– 200 9 15–20 lm ( x = 183.3 9 18.4 lm, n = 20), 8spored, bitunicate, fissitunicate, cylindrical to cylindricclavate, pedicellate, thick-walled at the apex, with minute ocular chamber. Ascospores 20–30 9 6–9 lm ( x= 25.9 9 6.6 lm, n = 50), overlapping uni-seriate, muriform, ellipsoidal to subfusiform, slightly curved, upper part 123 46 Fungal Diversity (2016) 80:1–270 Fig. 27 Tremateia guiyangensis (holotype). a, b Appearance of ascomata immersed on host surface. c Vertical section of ascoma. d Section of peridium. e Pseudoparaphyses. f–i Cylindrical-clavate asci. j–o Ascospores. Scale bars a = 500 lm, b = 100 lm, c = 50 lm, d, j–o = 10 lm, e–i = 20 lm wider than the lower part, 3–6 transversely septate, with 1 vertical septum, constricted at the septa, initially hyaline, becoming pale-brown at maturity, ends remaining coneshaped, with pointed upper ends and rounded lower ends, lacking a mucilaginous sheath or disappear with maturity. Asexual morph Undetermined. Culture characteristics: Colonies on MEA reaching 2 cm diam. after 30 days at 18 °C, circular, smooth margin 123 Fungal Diversity (2016) 80:1–270 Fig. 28 Phylogram generated from maximum Likelihood analysis (MEGA6) based on combined dataset of SSU and LSU rDNA sequences of the two Lentithecium strains and related taxa in the 47 family Lentitheciaceae. The tree is rooted to Dothidea sambuci. Maximum Likelihood bootstrap support values greater than 50 % are indicated. The new species are in blue and ex-types in bold 123 48 Fungal Diversity (2016) 80:1–270 Fig. 29 Lentithecium unicellulare (CBS H-22674, holotype). a, b Vertical sections of conidiomata. c Peridium. d–g Developing stages of conidia. h–k Conidia. Scale bars a, b = 50 lm, c = 15 lm, d–m = 10 lm white at first, dirty white after 4 weeks flat on the surface, without aerial mycelium, reverse greenish-grey. Hyphae septate branched, hyaline, thin, smooth-walled. Material examined: UK, England, Arun River, 6 April 2015, on herbaceous stem, E.B.G. Jones, GJ126 (MFLU 16-1275, holotype); (isotype in BBH) Notes: Tremateia arundicola resembles T. guiyangensis and T. halophila in having globose to subglobose ascomata with a thin peridium comprising cells of textura angularis and brown muriform ascospores. Both T. guiyangensis and T. halophila have comparatively shorter ( x = 150 lm) asci. They also have ascospores with more than 6 transverse septa and 2 vertical septa, while T. arundicola ascospores have less than 6 transverse septa and only one vertical septum. Tremateia guiyangensis J.F. Zhang, J.K. Liu, K.D. Hyde & Z.Y. Liu, sp. nov. 123 Index Fungorum number: IF552160; Facesoffungi number: FoF02235, Fig. 27 Etymology: Name reflects the place Guiyang, where the holotype was collected. Holotype: MFLU 16-1299 Saprobic on dead herbaceous stems. Sexual morph Ascomata 130–280 lm high, 190–400 lm diam., scattered to clustered, subglobose to ovoid, with flattened base, coriaceous, immersed to semi-immersed or breaking through the host epidermis, dark brown to black, ostiolate, minutely papillate. Peridium up to 9–16 lm, composed of several layers of light to brown, thick-walled cells of textura angularis, becoming thin-walled and hyaline towards the centrum. Hamathecium comprising numerous, 2.3– 5.1 lm wide, hypha-like, septate pseudoparaphyses, tapering towards the terminal cells, and intermingled among asci, embedded in a gelatinous matrix. Asci 152– 160 9 21–27 lm ( x = 149 9 24 lm, n = 20), 8-spored, Fungal Diversity (2016) 80:1–270 49 Fig. 30 Lentithecium voraginesporum (CBS H-22560, holotype). a Vertical section of ascoma. b Magnified part of the vertical section of the ascoma showing the peridium structure. c, d Mature asci. e, f Ascospores. Scale bars a = 50 lm, b–d = 10 lm, e, f = 5 lm bitunicate, fissitunicate, cylindrical-clavate, with a furcate pedicel, apically rounded, with a minute ocular chamber. Ascospores 20–28 9 9–12 lm ( x = 25 9 11 lm, n = 30), muriform, normally overlapping 1–2-seriate, ellipsoid to broad fusiform, both parts of the spore ±equal in size, with 3–5 transverse septa, and 1 longitudinal septum in each row, obviously constricted in the central septum and slightly constricted at other septa, initially hyaline, becoming golden-brownish to brown when mature, rounded at both ends and lacking any gelatinous sheath or appendages. Asexual morph Undetermined. Culture characteristics: Colonies on PDA reaching 3.5 cm diam. after 12 days at 25 °C, circular, raised at center, margin smooth, white at first, palely pigmented after 10 days at the center, reverse yellowish to reddish. Hyphae septate, branched, hyaline, thin, smooth-walled. Material examined: CHINA, Guizhou, Guiyang, around Guizhou Academy of Agriculture Sciences, on dead herbaceous stems, 17 August 2015, J.F. Zhang, GZ-01 (MFLU 16-1299, holotype), ex-type living culture, MFLUCC. Notes: Tremateia guiyangensis is phylogenetically close to T. arundicola and T. halophila, and also morphologically similar in having globose to subglobose or flattened, immersed to semi-immersed ascomata, with clavate asci and muriform ascospores. However, T. guiyangensis was collected from terrestrial habitat, while the other two species were collected from aquatic habitats. In addition, the asci of T. guiyangensis are shorter than T. arundicola ( x = 149 vs. 185 lm) and the ascospores are smaller than T. halophila ( x = 26 9 6.5 lm vs. 30 9 16 lm). There are more transverse and vertical septa in T. guiyangensis 123 50 Fungal Diversity (2016) 80:1–270 than in T. arundicola, and the ascospores of T. arundicola are paler than the other two species. Lentitheciaceae Yin. Zhang et al. Zhang et al. (2009b) established Lentitheciaceae to accommodate the saprobic genera Katumotoa, Keissleriella, Lentithecium and Tingoldiago described from aquatic and terrestrial habitats. The type genus of the family is the aquatic genus Lentithecium. Several new genera and species from different habitats have since been introduced in Lentitheciaceae based on molecular data. Quaedvlieg et al. (2013) established Setoseptoria to accommodate saprobic coelomycetes that are Septoria-like but with setose conidiomata and belong to Lentitheciaceae. Knapp et al. (2015) described the endophytic genus, Darksidea from semiarid sandy grasslands and multi-genes phylogenetic analyses placed Darksidea in Lentitheciaceae. Phookamsak et al. (2015) introduced Poaceascoma to accommodate a species with setose ascomata and filiform ascospores. Wijayawardene et al. (2015) introduced Fig. 31 RAxML maximum likelihood phylogenetic tree based on a LSU and ITS sequence data from species of Leptosphaeriaceae. Maximum likelihood bootstrap support values greater than 50 % are shown above the nodes. The extype strains are in bold and the new isolates are in red. The tree is rooted with Alternariaster helianthi Fig. 32 Leptosphaeria cirsii (holotype). a, b Ascomata on host. c c Section through the ascoma. d Peridium. e Pseudoparaphyses. f Mature ascus. g Immature ascus. h, i Ascospores. j Germinated ascospore. k Ascospores stained in Indian ink. l, m Culture from above (l) and below (m). n Fruiting body in culture. o Vertical section of conidioma. p Conidioma wall. q, r Conidiogenous cells and conidia s Conidia. Scale bars a = 500 lm, b = 200 lm, c = 100 lm, d, e–g, o, p = 30 lm, h–k = 10 lm, l–m = 4 cm, q– s = 5 lm Phragmocamarosporium Wijayawardene et al. to accommodate two coelomycetous species with brown phragmospores and phialidic conidiogenesis. The family currently includes 12 genera (Tanaka et al. 2015). In this paper we introduce two new species of Lentithecium and two new species of Poascoma and provide a new phylogenetic tree for Lentitheciaceae (Fig. 28). Lentithecium K.D. Hyde et al. The genus Lentithecium was established to accommodate Massarina arundinacea (Sowerby) Leuchtm., Leptosphaeria doliolum CBS 125979 Leptosphaeria doliolum CBS 541 66 54 Leptosphaeria doliolum CBS 505 75 Leptosphaeria doliolum MFLUCC 15-1875 Leptosphaeria veronicae CBS 126583 90 Leptosphaeria veronicae CBS 145 84 Leptosphaeria doliolum CBS 130000 Leptosphaeria doliolum CBS 155 94 62 71 Leptosphaeria errabunda CBS 617 75 errabunda CBS 125978 Leptosphaeria Leptosphaeria macrocapsa CBS 640.93 sensu stricto 79 Leptosphaeria sydowii CBS 385 80 50 99 Leptosphaeria sydowii CBS 125976 Leptosphaeria ebuli MFLUCC 14-0828 Leptosphaeria cichorium MFLUCC 14-1063 61 Leptosphaeria cirsii MFLUCC 14 1170 Leptosphaeria italica MFLUCC 15-0174 96 Leptosphaeria sclerotioides CBS 148 84 Leptosphaeria sclerotioides CBS 144 84 100 Leptosphaeria conoidea CBS 616 75 Leptosphaeria conoidea CBS 125977 98Leptosphaeria 100 82 Leptosphaeria slovacica CBS 125975 Leptosphaeria slovacica CBS 389 80 Leptosphaeria irregularis MFLUCC 15 1118 99 Leptosphaeria pedicularis CBS 126582 80 Leptosphaeria pedicularis CBS 390 80 Alloleptosphaeria italica MFLUCC 14-0934 Pseudoleptosphaeria etheridgei CBS 125980 100 Neoleptosphaeria rubefaciens CBS 387 80 Neoleptosphaeria rubefaciens CBS 223.77 Alternariaster helianthi CBS 327.69 100 0.1 123 Out group Fungal Diversity (2016) 80:1–270 51 123 52 123 Fungal Diversity (2016) 80:1–270 Fungal Diversity (2016) 80:1–270 b Fig. 33 Leptosphaeria irregularis (holotype). a, b Appearance of ascomata on host substrate. c Vertical section through ascoma. d Close up of the peridium. e Close up of the ostiole. f Pseudoparaphyses. g, h Asci. i–k Immature and mature ascospores. l Germinating ascospore. m, n Colonies on MEA. Scale bars b = 1 mm, c = 200 lm, d–h = 50 lm, i–i = 20 lm M. fluviatilis Aptroot & Van Ryck. and Keissleriella linearis E. Müll. ex Dennis. The genus currently contains seven species that were described from aquatic (Suetrong et al. 2009; Tanaka et al. 2015), or L. arundinaceum (Sowerby) K.D. Hyde et al. and L. rarum (Kohlm. et al.) Suetrong et al. from marine habitats. Lentithecium species have been described from decayed submerged parts of Phragmites (three species), Juncus (one species), and submerged wood (three species) (Zhang et al. 2009b; Suetrong et al. 2009; Tanaka et al. 2015). Lentithecium unicellulare Abdel-Aziz, sp. nov. Index Fungorum number: IF552267; Facesoffungi number: FoF02433, Fig. 29 Etymology: In reference to the unicellular conidia. Holotype: CBS H-22674. Saprobic on decayed wood in freshwater habitats. Sexual morph Undetermined. Asexual morph Conidiomata 115–235 lm high 9 140–235 wide lm ( x = 176.6 9 252.5 lm, n = 6), pycnidial, solitary or aggregated, uniloculate, immersed, erumpent to superficial, globose, subglobose, ovate, elongated, papillate, ostiolate, dark brown to black, surrounded by brown, septate, thick-walled hyphae, 3–4 lm wide. Pycnidial wall 18–25 lm thick, composed of 6–8 layers of yellow-brown to black brown cells of textura angularis, hyaline inner layer lining bearing conidiogenous cells. Conidiophores reduced to conidiogenous cells. Conidiogenous cells 9–14 9 8–12 lm ( x = 11.8 9 10.3 lm, n = 12), holoblastic, determinate, smooth-walled, hyaline, globose, subglobose to pearshaped with rounded or truncate base, sometimes proliferating once and each conidiogenous cell producing one to two conidia. Conidia 6–9 9 4–5 lm ( x = 8.1 9 4.3 lm, n = 50), subglobose, ovate, clavate, ellipsoid, allantoid, rectangular or irregular, with or without truncate base, hyaline, unicellular, smooth-walled. Culture characteristics: Colonies on PDA 45–50 mm diam. after 10 days at 23 °C, dark grey with irregular margins, reverse black, dense growth with aerial and submerged mycelia without diffusible pigments. Pycnidia were produced in pure cultures after 4 weeks. Dimensions of pycnidia and conidia were similar to those recorded from natural substrates. Material examined: EGYPT, Sohag City, on decayed wood submerged in the River Nile, 14 August 2014, F. 53 A. Abdel-Aziz (CBS H-22674, holotype), ex-type living culture MD 6004. Notes: Asexual genera reported so far under Lentitheciaceae are coelomycetous with variable morphology and these include: Phragmocamarosporium, Pleurophoma, Setoseptoria and Stagonospora macropycnidia (Tanaka et al. 2015). Lentithecium unicellulare differs from Stagonospora species in having globose, unicellular, holoblastic, determinate conidia that are smaller. Species of Stagonospora have cylindrical, multi-septate conidia, while conidiogenesis is holoblastic, occasionally annellidic, with single proliferation, discrete and indeterminate (Sutton 1980). The genus Stagonospora is polyphyletic and considered as the asexual morph of Phaeosphaeria (Leuchtmann 1984). Pleurophoma species differ from L. unicellulare in having long, filiform, septate, branched conidiophores and enteroblastic, phialidic, integrated, determinate conidiogenous cells (Sutton 1980). Setoseptoria species differ from L. unicellulare in having subcylindrical, transversely euseptate conidia, becoming constricted at septa and disarticulating into phragmospores when old. Conidiogenesis in Setoseptoria rarely has percurrent proliferations (Quaedvlieg et al. 2013). Lentithecium voraginesporum Abdel-Wahab, Bahkali & E.B.G. Jones, sp. nov. Index Fungorum number: IF 552266; Facesoffungi number: FoF02432, Fig. 30 Etymology: After the Latin word ‘‘Voraginem’’, meaning ‘‘gulf’’, where the fungus was recorded. Holotype: CBS H-22560. Saprobic on submerged wood in mangroves. Sexual morph Ascomata 125–215 lm diam., globose to subglobose, immersed to erumpent, ostiolate, papillate, coriaceous. Peridium 25–30 lm thick at the upper part around the ascomatal venter, two-layered, outer layer with yellowbrown cells forming a textura-angularis, inner-layer comprising thin-walled, hyaline, flattened cells; 11–17 lm thick at the basal part of the ascomata. Asci 38–50 3 8– 10 lm ( x = 44 3 9.3 lm, n = 15), 8-spored, bitunicate, clavate, short pedicellate, developing at the base of venter. Ascospores 15–21 3 5–6 lm ( x = 18.8 3 5.3 lm, n = 50), bi-seriate, yellow-brown to reddish-brown, ellipsoidal with rounded ends, 1-septate, septum sub-median, upper cell is longer and wider, with roughened surface. Asexual morph Undetermined. Culture characteristics: Colonies on PDA 20–25 mm diam. after 10 days at 23 °C, brown to dark-brown aerial and immersed mycelia with dark-brown reverse with circular margins, without diffusible pigments. Material examined: SAUDI ARABIA, Arabian Gulf, Tarut mangroves, on submerged, decayed Phragmites australis (Cav.) Trin. ex Steud. (Poaceae), stem inside the 123 54 123 Fungal Diversity (2016) 80:1–270 Fungal Diversity (2016) 80:1–270 b Fig. 34 Phylogram generated from maximum likelihood analysis based on combined LSU and SSU sequence data for species of Lindgomycetaceae. Maximum likelihood, Maximum parsimony bootstrap support values greater than 50 % and Bayesian posterior probabilities greater than 0.90 are near the nodes. The new isolates are in blue bold, and other ex-type strains are in black bold. The scale bar indicates 0.006 changes. The tree is rooted with Aigialus mangrovis and A. grandis mangrove stand, 28 March 2013, M.A. Abdel-Wahab (CBS H-22560, holotype), ex-type living culture MD 1342. Notes: Lentithecium voraginesporum is the third marine species in the genus and differs from the other seven described Lentithecium species in having small ascomata, asci and ascospores. Ascospores of the new species are characterized by their brown colour, roughened surface and the absence of a gelatinous sheath. The phylogenetic analyses of both SSU and LSU sequence data place L. voraginesporum within the family Lentitheciaceae in a wellsupported clade with the recently described species, L. cangshanense Z.L. Luo et al. and L. unicellulare AbdelAziz. Both species were described from freshwater habitats (Su et al. 2016b; this article). Lentithecium voraginesporum differs from L. cangshanense in having smaller ascomata (125–215 lm vs. 210–320 lm) and asci (38–50 3 8–10 lm vs. 65–78 3 11–13 lm), longer and narrower ascospores with a rough surface (15–21 3 5–6 lm vs. 16.5– 17.5 3 6–7 lm) and its marine habitat. Lentithecium unicellulare produces dark brown to black pycnidia and unicellular, hyaline conidia and its sexual morph is unknown. Molecular data delimit L. voraginesporum from L. cangshanense and L. unicellulare (Fig. 28). Lentithecium fluviatile differs from L. voraginesporum in having hyaline, longer and wider ascospores (24–31 3 7–10 lm vs. 15–21 3 5–6 lm) that are 2–3-septate and surrounded by a wide expanding mucilaginous sheath (van Ryckegem and Aptroot 2001). Lentithecium clioninum and L. pseudocloninum have larger asci and ascospores than those reported for L. voraginesporum. Ascospores of the latter two species are hyaline and surrounded by gelatinous sheath (Tanaka et al. 2015). Leptosphaeriaceae M.E. Barr The family Leptosphaeriaceae was established by Barr (1987) in the order Pleosporales and is typified by Leptosphaeria. Species of Leptosphaeriaceae can be saprobic, hemibiotrophic or parasitic on stems and leaves of herbaceous or woody plants in terrestrial habitats (Hyde et al. 2013). Members of this family are characterized by single, papillate, immersed or erumpent, perithecial ascomata, with relatively thick peridia, bitunicate cylindrical asci and hyaline to brown, transversely septate ascospores (Hyde et al. 2013). The asexual morphs of the family 55 Leptosphaeriaceae can be coelomycetous or hyphomycetous (Alves et al. 2013; De Gruyter et al. 2013; Hyde et al. 2013; Zhang et al. 2012b). In recent classifications Alternariaster, Heterospora, Leptosphaeria, Neophaeosphaeria, Paraleptosphaeria, Plenodomus, and Subplenodomus were included in the family (Ariyawansa et al. 2015b). In this study we introduce two new species within the genus Leptosphaeria. Leptosphaeria Ces. & De Not. For classification of Leptosphaeriaceae we follow Ariyawansa et al. (2015b) and provide an updated tree (Fig. 31). Leptosphaeria cirsii Jayasiri, Camporesi & K.D. Hyde, sp. nov. Index Fungorum number: IF552155; Facesoffungi number: FoF02220, Fig. 32 Etymology: The specific epithet circii is based on the host genus from which the taxon was collected. Holotype: MFLU 15-1072 Saprobic on dead stem of Cirsium sp. Sexual morph Ascomata 285–315 9 335–360 lm ( x = 304 9 347 lm, n = 10), solitary or scattered, superficial, globose to subglobose, broadly or narrowly conical, coriaceous, smoothwalled, ostiolate. Ostiole usually papillate, darkened at the base. Peridium 22–27 lm ( x = 24 lm, n = 20) wide, comprising two types of cells, outer cells of 1–2 layers of heavily pigmented cells of textura angularis, inner layer composed of small, light brown to hyaline cells of textura angularis. Hamathecium of septate, long, hyaline, cellular pseudoparaphyses, between and above the asci. Asci 75– 110 9 10–12 lm ( x = 95 9 11 lm, n = 20), 8-spored, bitunicate, fissitunicate, cylindro-clavate, with a short, broad pedicel, thickened and rounded at the apex, with a distinct ocular. Ascospores 19–22 9 4–4.5 lm ( x = 20 9 4.2 lm, n = 40), overlapping uni-seriate, hyaline to light brown when immature, becoming brown to chestnut brown when mature, ellipsoidal with narrowly rounded ends, 3-septate, smooth-walled with mucilaginous sheath. Asexual morph Coelomycetous on MEA. Conidiomata 150–200 9 195– 220 lm ( x = 190 9 205 lm, n = 10), pycnidial, superficial, immersed in media, globose to subglobose, black, without an ostiole. Conidiomata wall 15–25 lm wide, composed cells of textura angularis in multiple layers, pale yellowish-brown, remaining hyaline in inner layer. Conidiogenous cells 2–5 9 2–4 lm ( x = 3.5 9 2.5 lm, n = 30), enteroblastic, phialidic, determinate integrated, subglobose to short conical. Conidia 3–6 9 1–3 lm ( x = 3.8 9 1.5 lm, n = 50), hyaline, aseptate, oblong to cylindrical, thin-walled, smooth, guttulate, rounded at both ends. Culture characteristics: Colonies on MEA 60 mm diam. after 4 weeks at 18 °C, white on the top and reverse, 123 56 Fungal Diversity (2016) 80:1–270 Fig. 35 Arundellina typhae (holotype). a Appearance of immersed ascomata on host substrate. b Section of ascoma. c Peridium. d Pseudoparaphyses. e–h Asci. i–m Ascospores. Scale bars b = 50 lm, c, d = 10 lm, e–h = 20 lm, i–m = 10 lm 123 Fungal Diversity (2016) 80:1–270 Fig. 36 Lindgomyces pseudomadisonensis (holotype). a, b Appearance of ascomata on substrate. c Ascoma in longitudinal section. d Peridium in longitudinal section. e Asci. f Pseudoparaphyses. 57 g–j Ascospores. k Germinating ascospore. Scale bars a = 400 lm, b = 200 lm, c = 50 lm, d = 25 lm, e = 10 lm, f–k = 5 lm 123 58 123 Fungal Diversity (2016) 80:1–270 Fungal Diversity (2016) 80:1–270 b Fig. 37 RAxML tree based on analysis of a combined dataset of ITS, LSU, SSU and TEF partial sequences of Lophiostomataceae. Bootstrap support values for maximum likelihood (ML, black) higher than 50 % and Bayesian posterior probabilities (BYPP, red) greater than 0.95 are defined as above the nodes. The tree is rooted to Melanomma pulvis-pyrius. All type strains are in bold. New strains are given in blue reverse yellow to brown near the middle, some area dense, circular, regular margin, without diffusible pigments. Material examined: ITALY, Province of Trento [TN], near Vermiglio—Val di Sole, on dead stem of Cirsium sp. (Asteraceae), 9 August 2014, E. Camporesi IT 2044, (MFLU 15-1072, holotype); (KUN, isotype); ex-type living culture, MFLUCC 14-1170, KUNCC Notes: Leptosphaeria cirsii is introduced here based on both morphology and phylogeny. Leptosphaeria cirsii is typical of Leptosphaeria in having a peridium of plectenchymatous cells and reddish to yellowish-brown, fusoid, 3-septate ascospores. Phylogenetically, L. cirsii clusters with L. cichorium with relatively moderate support (61 % ML) and also shares affinities to L. italica. Morphologically L. cirsii is similar to L. cichorium in having similar asci, ascospores, cellular pseudoparaphyses and asexual state morphology, but differs in having, superficial, globose to subglobose, broadly or narrowly conical ascomata and ascospores with a mucilaginous sheath (Ariyawansa et al. 2015b). Leptosphaeria cirsii resembles L. italica but differs as L. cirsii has long and narrow ascospores and lacks an ostiolar canal (Dayarathne et al. 2015). Leptosphaeria irregularis R.H. Perera, E.B.G. Jones & K.D. Hyde, sp. nov. Index Fungorum number: IF552172, Facesoffungi number: FoF02234, Fig. 33 Etymology: Refers to the irregular shape of the fruiting body. Holotype: MFLU 16-1091 Saprobic on unidentified plant stem. Sexual morph Ascomata 245–370 lm high, 180–368 lm diam. ( x = 285 9 255 lm, n = 10), superficial, appearing as black raised spots on the host, solitary or arranged in groups of 2–5, globose to irregularly globose, uniloculate, black, ostiolate. Ostiole aperiphysate. Peridium 30–67 lm wide, composed of 8–16 rows of scleroplectenchymatous cells, outer layer of amorphous black cells, inner layers composed of hyaline to pale brown cells of textura angularis. Hamathecium comprising numerous, 1.5–2.2 lm (n = 25) wide, filamentous, branched, septate, pseudoparaphyses. Asci 110–147 9 6–8.5 lm ( x = 130 9 8 lm, n = 30), 8-spored, bitunicate, long-cylindrical, with a short furcate pedicel, rounded at the apex. Ascospores 23.5– 29 9 5–7 lm ( x = 27 9 6 lm, n = 40), uni-seriate to 59 overlapping uni-seriate, fusiform, 3-septate, constricted at the septa, initially hyaline, becoming reddish to yellowishbrown at maturity, conical at the ends, without a mucilaginous sheath. Asexual morph Undetermined. Culture characters: Colonies growing on MEA, reaching 4 cm diam. in 21 days at 16 °C, white, dense, moderate aerial mycelium on the surface, underneath pale yellow, margins even. Material examined: UK, Hampshire, Swanick Wood, on unidentified plant stem, 28 September 2015, E.B. Gareth Jones, GJ 199 (MFLU 16-1091, holotype), (isotype in HKAS); ex-type living cultures MFLUCC 15-1118, GAAS). Notes: Leptosphaeria irregularis is typical of Leptosphaeria in having a peridium of scleroplectenchymatous cells and 3-septate, reddish to yellowish-brown, fusiform ascospores. Phylogenies herein show that L. irregularis is a sister taxon to L. slovacica and can be considered distinct to other known Leptosphaeria species (Fig. 31). Morphologically L. irregularis is similar to L. slovacica, but differs in its larger (23.5–29 lm vs. 18–22 lm) ascospores. Lindgomycetaceae K. Hirayama et al. The family Lindgomycetaceae was introduced by Hirayama et al. (2010) for the freshwater genus Lindgomyces (L. breviappendiculatus, L. cinctosporae, L. ingoldianus and L. rotundatus) and its sister taxon, Massariosphaeria typhicola. Molecular phylogenetic studies using ribosomal sequence data from different lineages of Dothideomycetes also showed that Lindgomycetaceae is a unique lineage among the Pleosporales (Zhang et al. 2012b; Hyde et al. 2013; Wijayawardene et al. 2014b; Ariyawansa et al. 2015c; Wanasinghe et al. 2015). Currently there are 12 species included the genus Lindgomyces including the novel taxon introduced below. Multi-gene phylogenetic analyses also placed Arundellina typhae, Clohesyomyces aquaticus, Hongkongmyces pedis, Lolia aquatica, Massariosphaeria typhicola, Phyllosticta flevolandica and Trematosphaeria hydrela in Lindgomycetaceae (AbdelAziz and Abdel-Wahab 2010; Zhang et al. 2012b; Tsang et al. 2014; this study). Most of the Lindgomycetaceae members have been recorded from freshwater habitats, but Hongkongmyces is associated with IgG4-related sclerosing disease of humans (Tsang et al. 2014). Arundellina Wanasinghe, E.B.G. Jones & K.D. Hyde, gen. nov. Index Fungorum number: IF552132; Facesoffungi number: FoF02208. Etymology: Name reflects the Arun River, and town Arundel, from where the holotype was collected. Saprobic on dead stem, sheaths and leaves. Sexual morph Ascomata immersed, solitary, scattered, globose, dark brown to black, coriaceous, ostiolate. Ostiole 123 60 123 Fungal Diversity (2016) 80:1–270 Fungal Diversity (2016) 80:1–270 b Fig. 38 Vaginatispora fuckelii (HKAS 92495). a Herbarium mate- rial. b Appearance of ascomata on host. c Vertical section through ascoma. d Peridium. e–g Asci. h Pseudoparaphyses. i–k Ascospores. l Ascospore stained in Indian ink. m Germinating ascospore. n Upper view of culture on PDA. o Lower view of culture on PDA. Scale bars c = 50 lm, d, e–g, m = 20 lm i–l = 10 lm papillate, black, smooth, filled with hyaline to pale brown cells. Peridium thin, with 4–5 layers, outer layer heavily pigmented, thick-walled, comprising reddish to dark brown cells of textura angularis, inner layer composed of hyaline thin-walled cells of textura angularis. Hamathecium comprising numerous, filamentous, branched, septate pseudoparaphyses. Asci 8-spored, bitunicate, fissitunicate, cylindrical to cylindric-clavate, pedicellate, thick-walled at the apex, with an ocular chamber. Ascospores overlapping 1–2-seriate, fusiform, slightly curved, widest in the center, 3–4 transversely septate, without vertical septa, constricted at the septa, initially hyaline, becoming golden-pale brown at maturity, ends remaining cone-shaped, with pointed ends, without a mucilaginous sheath. Asexual morph Undetermined. Type species: Arundellina typhae Wanasinghe, E.B.G. Jones & K.D. Hyde Notes: The genus Arundellina is characterized by immersed, globose, ascomata, papillate ostiole, a thin peridium composed of cells of textura angularis, cylindrical to cylindric-clavate asci with a short pedicel and thick-walled apex and fusiform, golden-pale brown ascospores with 3–4 transverse septa and cone-shape pointed ends. The globose ascomata, cylindrical to cylindric-clavate asci and fusiform ascospores with 3–4 transverse septa of Arundellina resemble those of Equiseticola, Galiicola, Loratospora, Paraleptosphaeria and Phaeosphaeria in Phaeosphaeriaceae, Neolophiostoma in Halotthiaceae and Mytilinidion in Mytilinidiaceae. LSU and SSU combined gene phylogenetic analyses indicate that Arundellina belongs in Lindgomycetaceae, but is distinct from other genera in the family (Fig. 34). Arundellina typhae Wanasinghe, E.B.G. Jones & K.D. Hyde, sp. nov. Index Fungorum number: IF552133; Facesoffungi number: FoF02209, Fig. 35 Etymology: Name reflects the host genus Typha. Holotype: MFLU 16-1276 Saprobic on submerged stem and sheaths of Typhaceae sp. Sexual morph Ascomata 250–300 lm high 9 200– 250 lm diam. ( x = 272.1 9 229.5 lm, n = 5), immersed, solitary, scattered, globose, dark brown to black, coriaceous, ostiolate. Ostiole 70–90 lm high, 40–60 lm diam. ( x = 80.2 9 51.4 lm, n = 5), papillate, black, smooth, filled with hyaline to pale brown cells. Peridium 18–22 lm 61 wide at the base, 20–30 lm wide in sides, with 4–5 layers, outer layer heavily pigmented, thick-walled, comprising reddish to dark brown cells of textura angularis, inner layer composed of hyaline, thin-walled cells of textura angularis. Hamathecium comprising numerous, 2.5–3.5 lm wide, filamentous, branched, septate, pseudoparaphyses. Asci 110–115 9 15–20 lm ( x = 112.4 9 17.5 lm, n = 20), 8-spored, bitunicate, fissitunicate, cylindrical to cylindric-clavate, pedicellate, thick-walled at the apex, with an ocular chamber. Ascospores 30–40 9 5–7 lm ( x = 34.2 9 6.1 lm, n = 30), overlapping 1–2-seriate, fusiform, slightly curved, widest in the center, 3–4 transversely septate, without vertical septa, constricted at the septa, initially hyaline, becoming golden-pale brown at maturity, ends remaining cone-shaped, with pointed ends, without a mucilaginous sheath. Asexual morph Undetermined. Culture characteristics: Colonies on MEA reaching 2 cm diam. after 30 days at 16 °C, circular, margin smooth, dirty white at first, greenish dark brown after 4 weeks flat on the surface, without aerial mycelium, reverse blackish-brown. Hyphae septate branched, hyaline, thin, smooth-walled. Material examined: UK, England, Arun River, on dead submerged stem of Typha sp (Typhaceae), 6 April 2015, E.B.G. Jones, GJ122 (MFLU 16-1276, holotype); (isotype in BBH), ex-type culture, MFLUCC 16-0310, MUCL. UK, Dorset, West Lulworth, Lulworth Cove, 24 February 2015, on Typha sp., E.B.G. Jones, GJ096 (MFLU 16-1277, paratype); ex-paratype culture, MFLUCC 16-0309, MUCL. Lindgomyces pseudomadisonensis Tak. Takah. & Kaz. Tanaka, sp. nov. MycoBank number: MB817598; Facesoffungi number: FoF02434, Fig. 36 Etymology: Referring to its resemblance to Lindgomyces madisonensis. Holotype: HHUF 30513 Saprobic on submerged wood. Sexual morph Ascomata 190–240 lm high, 230–360 lm diam., globose to subglobose, black, scattered to grouped, immersed to erumpent. Ostiolar neck 90–100 lm long, 40–55 lm wide, papillate, central. Peridium 50–55 lm thick, composed of an inner layer of polygonal to subglobose, hyaline to pale brown cells and an outer layer of brown cells. Pseudoparaphyses cellular, numerous, 1.5–2.5 lm wide, anastomosed, branched. Asci 92.5–130 9 13.5–17.5 lm ( x = 108.3 9 15.6 lm, n = 8), fissitunicate, clavate, rounded at the apex, with an apical chamber, with eight overlapping bi-seriate ascospores. Ascospores 28–41(–45.5) 9 (5.5–)7–10 lm ( x = 36.3 9 7.9 lm, n = 63), l/w 3.7–6.1 ( x = 4.6, n = 63), fusiform with acute ends, straight or slightly 123 62 curved, with the primary septum almost supramedian (0.40–)0.42–0.51 ( x = 0.47, n = 62), slightly constricted at the primary septum, with a broad upper cell, hyaline, smooth, becoming 3-septate and pale brown with age, surrounded by an entire gelatinous sheath ca. 2 lm wide. Asexual morph Undetermined. Material examined: JAPAN, Aomori, Nishimeya, Seisyu trail, Ooshirosawa River, on submerged dead twigs of woody plant, 28 August 2010, K. Tanaka et al., KT 2742 (HHUF 30513, holotype); ex-type living culture, MAFF 245610. Notes: Lindgomyces pseudomadisonensis is morphologically similar to L. madisonensis and phylogeny also support a close association between them (Fig. 34). However, the former has shorter ascospores ( x = 39 ± 2 9 7 ± 1 lm). In addition, the ascospores of L. pseudomadisonensis have an entire gelatinous sheath. The identities of ITS sequences between L. pseudomadisonensis and L. madisonensis are rather low [GenBank KT207819; Identities = 541/564 (95.9 %), Gaps = 9/564 (1.5 %)]. Lophiostomataceae Sacc. The family Lophiostomataceae was introduced by Nitschke (1869) with Lophiostoma macrostomum (Tode) Ces. & De Not. as the type species (Eriksson 1981; Mugambi and Huhndorf 2009; Thambugala et al. 2015b). Most species in this family are widely distributed on twigs, stems, or bark of various woody plants and herbaceous plants in terrestrial and aquatic environments (Ellis and Ellis 1985; Mugambi and Huhndorf 2009; Zhang et al. 2009b; Hirayama and Tanaka 2011; Hyde et al. 2013; Thambugala et al. 2015b). Vaginatispora fuckelii (Sacc.) Thambugala, Wanasinghe, Kaz. Tanaka & K.D. Hyde, Fungal Diversity 74: 242. 2015. Index Fungorum Number: IF551535; Facesoffungi number: FoF00829 Basionym: Lophiostoma fuckelii Sacc., Michelia 1(no. 3): 336 (1878) = Lophiostoma pulveraceum Sacc., Michelia 1: 336, 1878 = Didymosphaeria lophospora Sacc. & Speg., Michelia 1: 376, 1878 = Lophiosphaera mendax Rehm, Ann. Myc. 5: 544, 1907 Saprobic on dead branch of Rosa sp. Sexual morph Ascomata 145–205 lm high, 140–258 lm diam., solitary, scattered to clustered, immersed to semi-immersed or erumpent, globose to subglobose, glabrous, uniloculate, rarely bi-loculate, papillate, visible as raised, black spots on host surface. Peridium 18–26 lm wide, thin-walled, unevenly thickened, two layered; inner layer comprising 3– 5 cell layers of flattened, hyaline cells of textura 123 Fungal Diversity (2016) 80:1–270 Fig. 39 Phylogram generated from maximum parsimony analysis c based on combined LSU, SSU and TEF1a sequence data of Lophiotremataceae. Maximum likelihood (left)/ parsimony (right) bootstrap support values greater than 50 % are shown above the nodes. The ex-type strains are in bold and the new species are indicated in blue. The tree is rooted to Dothidotthia symphoricarpi and D. aspera prismatica; outer layer comprising several layers of dark brown to black cells of textura angularis. Hamathecium composed of dense, 1.7–2.5 lm wide, filamentous, indistinctly septate, cellular pseudoparaphyses, anastomosing at the apex, embedded in a hyaline gelatinous matrix. Asci 43–72 9 4.6–6.8 lm ( x = 57 9 5.6 lm, n = 20), 8spored, bitunicate, fissitunicate, cylindric-clavate, short pedicel with furcate to obtuse ends, apically rounded with an indistinct ocular chamber. Ascospores 15–23 9 3– 4.7 lm ( x = 19.7 9 3.8 lm, n = 30), overlapping bi-seriate, hyaline, subfusoid, with rounded or obtuse ends, initially aseptate, becoming 1-septate at maturity, strongly constricted at the septum, enlarged near the septum at the upper cell, smooth-walled, guttulate, surrounded by thin distinctive sheath when immature, invisible at maturity, bearing appendages at both ends. Asexual morph Undetermined. Culture characteristics: Colonies on PDA, 33–35 mm diam. after 3 weeks, colonies medium dense, irregular, flat, slightly raised, surface smooth with crenate edge, fluffy to velvety with smooth aspects, zonate with different sector yellowish-grey to yellowish-brown at the margin whitishgrey to brownish-grey at the centre; reverse whitish-grey at margin, yellowish-brown with fimbriate aspect at the midlle, black at the centre, no pigmentation produced in media. Material examined: CHINA, Yunnan Province, Kunming, on dead branch of Rosa sp. (Rosaceae), 9 June 2015, Wen Jing Li, NI005 (HKAS 92495); living culture KUMCC 15-0523. Notes: This is the first record of Vaginatispora fuckelii on dead branch of Rosa species (Rosaceae) in China. The current maximum likelihood analysis shows that the new strain (KUMCC 15-0523) clusters with other Vaginatispora fuckelii strains with low bootstrap support (Fig. 37). The new strain, however, differs from the others in asci and ascospores size. Vaginatispora fuckelii has 60–90 9 9–12 lm asci and 15–17 9 4–5 lm ascospores (Thambugala et al. 2015b), whereas the new strain has 43–72 9 4.6–6.8 lm asci and 15–23 9 3–4.7 lm ascospores. Vaginatispora fuckelii has been recorded from various dead herbaceous twigs including Vitis coignetiae (Thambugala et al. 2015b) and also occurs on leaves of Mangifera indica (Wang and Lin 2004). The known distribution of this species is Sweden, UK, Germany, Fungal Diversity (2016) 80:1–270 63 123 64 123 Fungal Diversity (2016) 80:1–270 Fungal Diversity (2016) 80:1–270 b Fig. 40 Hermatomyces subiculosa (holotype). a Host (decaying wood). b, c Subiculum, conidiophores and conidia on the host surface, (1) subiculum (2) conidia. d Infertile hyphae. e Conidiophores. f Conidiophores, conidiogenous cells and conidia, (3) parts of the conidiogenous cells, (4) conidiophores. f–h Conidia. l, m 20-day old colonies on MEA, l from above, m from below. Scale bars b = 500 lm, c = 200 lm, d–f = 20 lm, g–k = 10 lm Switzerland, Taiwan and Japan (Wang and Lin 2004, Thambugala et al., 2015b). Vaginatispora fuckelii is distinct from other Vaginatispora species in having 1-septate, Fig. 41 Lophiotrema bambusae (MFLU 11-0150, holotype). a Appearance of ascostromata on the host surface. b Section through the ascostroma. c Section through peridium. d Pseudoparaphyses stained in cotton blue. e–h Asci. i Ocular chamber stained in Melzer’s 65 guttulate, ascospores with a sheath and appendages at both ends (Fig. 38). Lophiotremataceae K. Hiray. & Kaz. Tanaka The family Lophiotremataceae was introduced by Hirayama and Tanaka (2011) to accommodate a single genus Lophiotrema, typified by L. nucula (Fr.) Sacc. Lophiotrema shares morphological characters with Lophiostoma due to its carbonaceous ascomata with compressed, crest-like apex and was previously regarded as a synonym of Lophiostoma (Chesters and Bell 1970; Zhang et al. reagent. j–l Ascospores. m Ascospores stained in Indian ink. n, o Culture characteristics (n = from above, o = from below). Scale bars b = 100 lm, c–h = 20 lm, i = 10 lm, j–m = 5 lm 123 66 Fig. 42 Lophiotrema fallopiae. a, b Appearance of ascomata on substrate. c Ascoma in longitudinal section. d Peridium of ascoma. e, f Ascus. g Ascus apex. h Pseudoparaphyses. i–k Ascospores. l Ascospore with gelatinous sheath (in Indian ink). m Germinating ascospore. n, o Conidiomata in culture. p Conidioma in longitudinal 123 Fungal Diversity (2016) 80:1–270 section. q Peridium of conidioma. r, s Conidiogenous cells. t, u Conidia. v Germinating conidium. a–m From HHUF 30506 (holotype); n–v from MAFF 245612 (ex-type culture). Scale bars a = 1 mm, b, n, o = 250 lm, c, p = 20 lm, d–f, q = 10 lm, g–m, r–v = 5 lm Fungal Diversity (2016) 80:1–270 67 Fig. 43 Phylogram generated from RAxML analysis of combined LSU, SSU and TEF sequence data of Massariaceae. Maximum Likelihood (ML) bootstrap support values greater than 50 % and Bayesian posterior probabilities (PP) greater than 0.95 are shown above and below branches. The ex-type strains are in bold and the new isolate is in blue. The tree is rooted with Acrospermum gramineum and A. compressum 2009b, 2012b; Hirayama and Tanaka 2011; Hyde et al. 2013). However, these two genera can be distinguished by their peridial structure and shape of ascus (Zhang et al. 2009a, b; Hirayama and Tanaka 2011, Hyde et al. 2013). The peridial structure of Lophiotrema is usually thin, of equal thickness, composed of cells of textura angularis to textura globulosa and asci are often oblong to cylindrical with hyaline ascospores (Zhang et al. 2009a, b; Hirayama and Tanaka 2011; Hyde et al. 2013). In Lophiostoma the peridium is of unequal thickness and is usually broader near the base, while asci are mostly clavate (Zhang et al. 2009b; Hirayama and Tanaka 2011). Doilom et al. (2016) 123 68 Fig. 44 Neomassaria fabacearum (holotype). a, b Appearance of ascomata on substrate. c Section through ascoma. d Ostiole. e Peridium. f Pseudoparaphyses. g, h Asci. i–k Ascospores. l Ascospores 123 Fungal Diversity (2016) 80:1–270 surrounded by hyaline gelatinous sheath in Indian ink. Scale bars a = 500 lm, b = 200 lm, c, g–h = 50 lm, d = 20 lm, e, f, i– l = 10 lm Fungal Diversity (2016) 80:1–270 69 Fig. 45 Phylogram generated from maximum likelihood analysis based on combined LSU, ITS, SSU, and TEF1-a sequence data from species of Massarinaceae. Maximum likelihood bootstrap support values greater than 50 % and Bayesian posterior probabilities greater than 0.90 are shown above the nodes. Ex-type strains are in bold and the new isolate is in blue. The tree is rooted with Periconia digitata accepted two other genera, Hermatomyces and Aquasubmersa in the Lophiotremataceae as in the phylogenetic analyses these two genera are related to Lophiotrema. Species in Lophiotrema are not well-studied and lack modern taxonomic treatments and molecular data. There are 159 epithets recorded in Index Fungorum (2016), but only 96 sequences from six species in GenBank. In this study, we introduce two new taxa, Lophiotrema bambusae and L. fallopiae. We also provide a new combination for Scyphostroma mirum under Hermatomyces mirum and introduce H. subiculosa sp. nov. = Subicularium reticulatum M.L. Farr & Goos, Mem. N. Y. bot. Gdn 49: 66 (1989) Index Fungorum number: IF552284; Facesoffungi number: FoF02435 Notes: Farr and Goos (1989) introduced a monotypic genus, Subicularium, with S. reticulatum M.L. Farr & Goos as type, and provisionally placed the genus in the order Agonomycetales of Deuteromycotina. The Dictionary of Fungi (Kirk et al. 2008) recorded S. reticulatum under an older name Scyphostroma mirum Starbäck (Starbäck 1899), apparently based on their similar morphology, while Seifert et al. (2011) treated them differently. Incidentally, the genus Scyphostroma Starbäck, typified by Sc. mirum Starbäck, was found to be wrongly described by the original author (Starbäck 1899). The description ran as follows: ‘‘Subiculum dark-brown, with reticulately branched hyphae, forming a cup-like nest with a distinct, thick Hermatomyces mirum (Starbäck) C.G. Lin, D.J. Bhat, Yong Wang bis & K.D. Hyde, comb. nov. Basionym: Scyphostroma mirum Starbäck, Bih. K. svenska Vetensk Akad. Handl., Afd. 3 25(no. 1): 23 (1899) 123 70 123 Fungal Diversity (2016) 80:1–270 Fungal Diversity (2016) 80:1–270 b Fig. 46 Stagonospora forlicesenensis (holotype). a Appearance of conidiomata on host surface. b Conidioma on host surface. c Vertical section of conidioma. d Basal mycelium. e Setae at side of conidioma. f Pycnidial walls. g–i Developing stages of conidia. j, l Conidia with campanulate appendage. k Conidia stained with cotton blue reagent. m, n Culture characters on PDA. Scale bars b = 200 lm, c = 100 lm, d, e, h, i, j = 20 lm, f = 50 lm, g, j, k = 10 lm Fig. 47 Phylogenetic tree generated by maximum likelihood (RAxML) analysis of combined LSU, SSU, RPB2 and EF-1a sequence data from species of Melanommataceae. Bootstrap support values for maximum likelihood equal or greater than 50 % are given above the nodes. Bayesian posterior probabilities equal or greater than 71 margin. Perithecia minute, sphaerical, with distinct stalk, perched in the dense stromatic cup’’. Starbäck (1899) mistook the conidia for perithecia. The species description indicated that the cup-like conidiomata measured 0.5–4 mm diam. and conidia (wrongly referred as 0.90 are given below the nodes. Ex-type strains, reference strains and new isolates are in bold. Newly generated sequences are in blue. The tree is rooted with Hysterium angustatum CBS 123334 and H. angustatum 123 72 Fig. 48 Bertiella ellipsoidea (holotype). a Herbarium material. b Ascomata on wood. c Ascoma on wood. d, e Cross section of an ascoma. f Vertical section of the ascoma at margin. g Septate hairs. h Aseptate, branched pseudoparaphyses. i–l Cylindrical asci. m Apical 123 Fungal Diversity (2016) 80:1–270 apex. n–q Ovoid ascospores. Scale bars b = 500 lm, c, h = 100 lm, d = 400 lm, e = 200 lm, f = 70 lm, g = 50 lm, i–l = 40 lm, m = 25 lm, n–q = 10 lm Fungal Diversity (2016) 80:1–270 ‘perithecia’) measured 40–50 lm diam. It is clear that Hermatomyces is the same as Scyphostroma. Although Scyphostroma is the first name for the genus, we preferred to use Hermatomyces because the latter name is presently in use and the earliest name was wrongly described in the literature and has never been referred to any taxonomic discourses of the genus complex. Accordingly, in this study, we propose the synonymy of Scyphostroma mirum and Subicularium reticulatum, under the new combination Hermatomyces mirum, based on their morphological similarities and phylogenetic analysis (Fig. 39). Hermatomyces subiculosa C.G. Lin, D.J. Bhat, Yong Wang bis & K.D. Hyde, sp. nov. Index Fungorum number: IF552285, Facesoffungi number: FoF02436, Fig. 40 Etymology: Referring to the conspicuous subiculum on the host surface. Holotype: MFLU 16-1300. Saprobic on decaying wood. Sexual morph Undetermined. Asexual morph Colonies subiculate on natural substrate, superficial, effuse, floccose, greyish to dark brown. Mycelium superficial, composed of septate, flexuous, repeatedly branched, pale to brown, 2–4 lm wide infertile hyphae. Conidiophores micronematous or semimacronematous, mononematous, brown, smooth, confined to the center and surrounded by infertile hyphae, 3–5.5 lm wide. Conidiogenous cells holoblastic, subhyaline, 3– 5.5 lm wide. Conidia solitary, dry, acrogenous, muriform, deeply constricted at septa, globose, oblong, ellipsoidal, pyriform, verruculose, subhyaline to pale brown when young, dark brown when mature, 15–35 lm ( x = 26.23 lm, n = 39) long, 18–30 lm ( x = 24.28 lm, n = 39) wide at the broadest part, sometimes with part of conidiogenous cell attached. Culture characteristics: Colonies on MEA attaining a diam. of 2.5–3.5 cm at room temperature (25 °C) in 3 weeks, effuse, hairy, grey above, pale brown at the margins, dark brown at the center from below. Material examined: THAILAND, Chiang Rai, Mae Sai District, Ang Kep Nam Wat Tham Khao Hin Phayanak (Wat Tham Sao Hin Payanak), 20°190 16.5800 – 20°190 30.1200 N, 99°510 40.7200 –99°510 54.5000 E, on decaying wood, 19 June 2015, Chuan-Gen Lin, WTSP 1-1 (MFLU 16-1300, holotype; HKAS 95052, isotype), ex-type living culture MFLUCC 15-0843. Notes: We found that our new species forms a separate well-supported clade within the genus Hermatomyces, sister to H. thailandica (MFLUCC 14-1143, MFLUCC 14-1145 and MFLUCC 14-1144) and H. tectonae (MFLUCC 14-1140 and MFLUCC 14-1141) in the family Lophiotremataceae. The new species is morphologically 73 similar to H. mirum in having a conspicuous subiculum and the conidia of these two species are hyaline to pale brown when young, and dark brown when mature. However, the conidia in our new species (18–30 lm) are smaller than H. mirum (40–50 lm) verruculose and, in addition, the subiculum of H. mirum are more or less disc-shaped, with individual discs 500–4000 lm in diam., while the subiculum in the new species are effuse on the natural substrate. Therefore, based on differences in morphology and molecular data, we introduce the new species, Hermatomyces subiculosa. Lophiotrema bambusae Phookamsak, S.C. Karunarathana & K.D. Hyde, sp. nov. Index Fungorum number: IF552203; Facesoffungi number: FoF02257, Fig. 41 Etymology: The specific epithet ‘‘bambusae’’ refers to the host. Holotype: MFLU 11-0150. Saprobic on bamboo. Sexual morph Ascostromata 140– 270 lm high, 250–370 lm diam., dark brown to black, scattered, gregarious, immersed to semi-immersed in host cortex, raised, conical to quadrilateral, or irregular in shape, uni-loculate, glabrous, coriaceous, ostioles central or lateral, with rim-like opening. Peridium 20–50 lm wide, thin- to thick-walled, of unequal thickness, slightly thick at the sides towards apex, composed of several layers of small, dark brown to black, pseudoparenchymatous cells, with host cells plus fungal tissue, arranged in a textura angularis to textura epidermoidea. Hamathecium composed of dense, 2–3 lm wide, cellular pseudoparaphyses, distinctly septate, anastomosing among the asci, embedded in a hyaline gelatinous matrix. Asci 65–100 9 8–10 lm ( x = 83.3 9 8.7 lm, n = 20), 8-spored, bitunicate, fissitunicate, cylindrical, short pedicellate, apically rounded with an ocular chamber. Ascospores 18–25 9 4–5 lm ( x = 21.3 9 4.4 lm, n = 25), overlapping bi-seriate, hyaline, fusiform, with rounded ends, 1-septate, rarely 3–5septate, upper cell larger that lower cell, constricted at the central septum, smooth-walled, with guttules, surrounded by a mucilaginous sheath. Asexual morph Undetermined. Culture characteristics: Colonies on PDA reaching 39–45 mm diam. after 4 weeks at 25–30 °C; colony from above, dark green, with black concentric ring at the margin, black in the centre, with white turfs; from below, dark green, with black concentric ring at the margin, dark green to black at the centre, slightly radiating; medium dense, irregular, flattened, slightly raised at the middle, edge undulate, with entire margin, surface smooth, slightly rough, with white tufts, or black granules, woolly; not producing pigmentation in agar. Material examined: THAILAND, Chiang Rai, Mae Fah Luang District, Doi Tung, on dead stem of bamboo, 28 123 74 April 2010, S.C. Karunarathana, RP0030 (MFLU 11-0150, holotype), ex-type living cultures, MFLUCC 10-0558, BBC. Notes: Lophiotrema bambusae shares a size range of asci and ascospores, including number of ascospore septa with many Lophiotrema species, such as L. alpinum (Ellis & Everh.) M.E. Barr, L. arundinariae Rehm, L. culmifragum Speg., L. incisum Ellis & Everh., L. paspalicola Speg. and L. radicans (Ellis & Everh.) Sacc. However, the concept of Lophiotrema was unclear, when many of these species were introduced or transferred to the genus and they need restudying. The ascospore septation of L. bambusae is typically 1-septate (rarely 3–5-septate), similar to L. alpinum, L. culmifragum and L. incisum, while, L. arundinariae, L. paspalicola and L. radicans usually have 3-septate ascospores. Nevertheless, L. bambusae differs from L. alpinum and L. incisum based on host, with L. bambusae collected from bamboo, and the others collected from conifer and Ribes. Lophiotrema bambusae is most similar to L. culmifragum given its host and habitat. However, L. bambusae has slightly larger asci and mature ascospores have more septa. Phylogenetic analyses show that L. bambusae is related to L. lignicola and distinct from other taxa in Lophiotrema (Fig. 39). Therefore, the new species is established. Lophiotrema fallopiae A. Hashim. & Kaz. Tanaka, sp. nov. MycoBank number: MB817597; Facesoffungi number: FoF02475, Fig. 42 Etymology: Referring to the generic name of host plant. Holotype: HHUF 30506. Saprobic on dead stem of Fallopia japonica. Sexual morph Ascomata 200–300 lm high, 180–250 lm diam., scattered, immersed, erumpent at the neck, subglobose in section. Ostiolar neck up to 40 lm high, compressed, composed of carbonaceous, black, thick-walled cells, without clypeus. Peridium 17.5–25.5 lm thick, 5–8 layers, composed of polygonal to elongate, thin-walled, 8–13 9 2.5– 3 lm cells. Hamathecium comprises branched pseudoparaphyses numerous, trabeculate, 1–1.5 lm wide, septate. Asci (77–)93–125 9 (5.5–)7–9 lm ( x = 105.8 9 7.6 lm, n = 10), 8-spored, numerous, bitunicate, fissitunicate, cylindrical, with a short stipe (5–11 lm long, x = 7.4 lm, n = 10), apically rounded with an ocular chamber. Ascospores 19–24 9 4.5–6 lm ( x = 21.7 9 5.2 lm, n = 50), l/w 3.7–5.3 ( x = 4.2, n = 50), fusiform with rounded ends, straight, 1-septate, constricted, with a primary septum nearly median [0.45–0.55, x = 0.51, n = 50], hyaline, smooth, guttulate when young, with an entire gelatinous sheath (2.5–6 lm wide at sides). Asexual morph 123 Fungal Diversity (2016) 80:1–270 Coelomycetes. Conidiomata pycnidial, globose to subglobose, up to 200 lm high in section, 120–180 lm diam., scattered, semi-immersed, solitary, black. Peridium 10– 14 lm wide; outer layers composed of 7.5–10.5 9 4– 7.5 lm, subglobose, brown cells; inner layers composed of 5–7.5 9 0.5–1.5 lm, ‘‘porrecta’’, hyaline cells. Conidiophores reduced. Conidiogenous cells enteroblastic, phialidic, 7–9 9 2–4 lm, lageniform, hyaline, smooth. Conidia ellipsoidal with rounded ends, 2–3.5 9 1–2 lm ( x = 2.7 9 1.5 lm, n = 60), l/w 1.2–2.5 ( x = 1.8, n = 60), hyaline, aseptate, smooth. Culture characteristics: Colonies on PDA attaining 12–16 mm diam. within 21 days at 20 °C in the dark. Material examined: JAPAN, Aomori, Hirakawa, Kuzukawa, near Aseishi River, on dead stem of Fallopia japonica, 5 September 2010, K. Tanaka, KT 2748 (HHUF 30506, holotype); ex-holotype living culture, MAFF 245612. Notes: The characters of L. fallopiae fit into the generic concept of Lophiotrema in having compressed ostiolar necks, a peridium composed of textura angularis, and cylindrical asci with short stipes. This species is similar to L. nucula, the type species of Lophiotrema, but L. nucula has shorter ascospores [17–21(–25) 9 (4–)5–6.5 lm; Zhang et al. 2009a]. Lophiotrema fallopiae is phylogenetically close to L. vagabundum (Fig. 39), but L. vagabundum has slightly narrower ascospores (20–26 9 4–5.5 lm, l/w 4.3–5.9; Tanaka and Harada 2003). Massariaceae Nitschke The family Massariaceae was introduced by Nitschke (1869) to accommodate the genus Massaria with M. inquinans (Tode) De Not. as the type species. The family is characterized by immersed globose, subglobose to pyriform, ascomata, with a thin-walled peridium comprising cells of textura angularis, oblong to cylindrical asci with a wide ocular chamber and refractive ring, and large, oblong to ellipsoidal ascospores, surrounded by a gelatinous sheath (Hyde et al. 2013). Seven new species were introduce in Massaria with molecular analyses of combined LSU, SSU, RPB2 and TEF1 sequence data. In this study, the new genus Neomassaria is introduced based on molecular and morphological comparison with descriptions and illustrations. The phylogenetic tree, based on combined LSU, SSU and TEF1 sequence data for the new taxon is presented in Fig. 43. Neomassaria Mapook, Camporesi & K.D. Hyde, gen. nov. Index Fungorum number: IF552273; Facesoffungi number: FoF02437 Etymology: The generic epithet refers to a new genus in the family Massariaceae. Fungal Diversity (2016) 80:1–270 75 Neotrematosphaeria biappendiculata KTC 1124 Coelodictyosporium muriforme MFLUCC 13-0351 Lophiopoacea paramacrostoma MFLUCC 11-0463 Sigarispora ravennica MFLUCC 14-0005 Lophiohelichrysum helichrysi MFLUCC 15-0701 Guttulispora crataegi MFLUCC 13-0442 Lophiostomataceae Platystomum crataegi MFLUCC 14-0925e Lophiostoma compressum IFRD 2014 Lophiostoma macrostomum KT508 Biappendiculispora japonica MAFF 239452 79 Capulatispora sagittiformis JCM 15100 93 Platystomum scabridisporum BCC 22835 Trematosphaeria terricola SC 12 85 96 Paucispora quadrispora MAFF 239455 Dimorphiopsis brachystegiae CPC 22679 Vaginatispora aquatic MFLUCC 11-0083 98 Angustimassarina populi MFLUCC 13-0034 75 Massarina corticola CBS 154.93 Amoroslaceae 100 Angustimassarina quercicola MFLUCC 14-0506 Angustimassarina acerina MFLUCC 14-0505 Exosporium stylobatum CBS 160.30 Camarographium carpini CBS 128781 99 Preussia lignicola CBS 264.69 94 Sporormia lignicola CBS 363.69 Preussia minima AFTOL ID1256 90 Preussia funiculate 2577 100 Sporormiaceae Preussia funiculate CBS 659.74 Preussia terricola AFTOL ID 282 Westerdykella dispersa CBS 50875 74 Westerdykella ornate CBS 379 55 98 97 Eremodothis angulate CBS 610.74 Westerdykella cylindrical CBS 454.72 Sporormia fimetaria Gr.81.194 100 Decaisnella formosa BCC 25617 97 Decaisnella formosa BCC 25616 100 Lignosphaeria thailandica MFLUCC 11-0376 Lignosphaeria fusispora MFLUCC 11-0377 100 Berkleasmium nigroapicale BCC 8220 Bahusandhika indica GUFCC 18001 100 Misturatosphaeria kenyensis GKM L100Na Pseudoaurantiascoma kenyense GKM 1195 95 Misturatosphaeria minima ANM 933 Misturatosphaeria minima GKM 169N Curreya grandicipis CBS 114272 Misturatosphaeria claviformis GKM 1210 Macrodiplodiopsis desmazieri MFLUCC 12-0088 6 Floricolaceae Floricola striata JK 5678I Floricola striata JK 5603K Misturatosphaeria tennesseensis ANM 911 Pseudomisturatosphaeria cruciformis SMH 5151 Floricola viticola IT 2178 Misturatosphaeria aurantonotata GKM 1238 Misturatosphaeria radicans ATCC 42522 Ramusculicola thailandica MFLUCC 13-0284 Neomassarina thailandica MFLUCC 10 0552 93 Pontoporeia biturbinata BBH 23338 4x 100 Halotthia posidoniae BBH 22481 Mauritiana rhizophorae BCC 28866 Halotthiaceae Sulcosporium thailandica MFLUCC 12-0004 Neolophiostoma pigmentatum MFLUCC 10-0129 Brunneoclavispora bambusae MFLUCC 11-0177 Roussoellopsis tosaensis KT 1659 Roussoella pustulans KT 1709 Roussoella hysterioides HH 26988 95 Arthopyrenia salicis CBS 368.94 Roussoellaceae Arthopyrenia salicis CBMAI1330 74 Roussoella angustior MFLUCC 15-0186 Roussoella magnatum MFLUCC 15-0185 Neoroussoella bambusae MFLUCC 11-0124 Sporidesmium_australiense 100 Torula herbarum CBS 111855 98 Torula herbarum CBS 379.58 Torula herbarum CBS 220.69 Torulaceae 100 Dendryphion europaeum CPC 22943 Dendryphion europaeum CPC 23231 100 Parameliola acaciae MFLU 15-0378 Parameliola dimocarpi MFLU15-0045 100 Pseudocoleodictyospora tectonae MFLUCC 12-0387 72 Pseudocoleodictyospora_tectonae MFLUCC 12-0385 Pseudocoleodictyospora thailandica MFLUCC 12-0565 100 Pseudocoleodictyospora sukhothaiensis MFLUCC 12-0554 Pseudocoleodictyosporaceae Subglobosporium tectonae MFLUCC 12-0393 100 Subglobosporium tectonae MFLUCC 12-0390 100 98 100 97 98 Fig. 49 RAxML Maximum lkelihood phylogenetic tree based on a LSU and SSU sequence data from species of order Pleosporales. Maximum likelihood bootstrap support values greater than 50 % are shown on near the nodes. Some branches were shortened to fit the page—these are indicated by two diagonal lines with the number of times a branch was shortened indicated next to the lines. The new isolates are in red. The tree is rooted with Hysterium angustatum 123 76 Fungal Diversity (2016) 80:1–270 84 Occultibambusa bambusae MFLUCC 13-0855 Occultibambusa bambusae MFLUCC 11-0394 Occultibambusa chiangraiensis MFLUCC 16-0380 Occultibambusa aquatica MFLUCC11-0006 Occultibambusa pustula MFLUCC 11-0502 Occultibambusaceae Versicolorisporium triseptatum JCM 14775 Occultibambusa fusispora MFLUCC 11-0127 100 Seriascoma didymospora MFLUCC 11-0179 Seriascoma didymospora MFLUCC 11-0194 Neooccultibambusa chiangraiensis MFLUCC 12-0559 94 _Biatriospora mackinnonii CBS 674.75 100 Nigrograna mackinnonii E5202H Biatriosporaceae Biatriospora marina CY 1228 100 Paradictyoarthriinium diffractum MFLUCC 12-0557 Paradictyoarthriiniaceae Paradictyoarthriinium tectonicola MFLUCC 13-465 Stagonospora pseudocaricis HKUCC 10833 Neottiosporina paspali CBS 331.37 Corynespora leucadendri CBS 135133 Massarinaceae Corynespora olivacea CBS 114450 Massarina eburnea CBS 473.64 Pseudodidymosphaeria phlei MFLUCC14-1061 94 Deniquelata barringtoniae MFLUCC 11-0422 Kalmusia brevispora KT 1466 85 Montagnula aloes CPC 19671 Didymosphaeriaceae 100 Pseudocamarosporium pini MFLUCC 14-1091 Didymosphaeria rubi-ulmifolii MFLUCC 14-0023 96 Bambusicola splendida MFLUCC 11-0439 Bambusicolaceae Bambusicola massarinia MFLUCC 11-0389 100 Latorua caligans CBS 576.65 Latoruaceae Latorua grootfonteinensis CBS 369.72 86 Katumotoa bambusicola KT 1517a Lentitheciaceae Lentithecium fluviatile CBS 122367 Pseudomonodictys tectonae MFLUCC 12-0552 79 Trematosphaeria pertusa CBS 122371 Trematosphaeriaceae Falciformispora lignatilis BCC 21118 Macrodiplodiopsis desmazieri CPC 24971 99 Macrodiplodiopsis desmazieri CBS 125026 Macrodiplodiopsisceae Macrodiplodiopsis desmazieri CPC 24648 100 Moroshperia ramunculicola BCC 18404 85 Moroshperia ramunculicola BCC 18405 Moroshperiaceae Helicascus nypae BCC 36752 Longiostiolum tectonae MFLUCC 12 0562 Thyridaria rubronotata CBS 385.39 Thyridariaceae Massariosphaeria phaeospora CBS 611.86 100 Halojulella avicenniaea BCC 18422 Halojulellaceae Halojulella avicenniae BCC 20173 100 Cucurbitaria berberidis CBS 363.93 82 Cucurbitaria berberidis CBS 394.84 Cucurbitariaceae Pyrenochaeta nobilis CBS 407.76 89 Camarosporium quaternatum DQ377884 Camarosporium aloes CPC 21572 Phaeosphaeria oryzae CBS 110110 94 79 Ophiosphaerella herpotricha AFTOL-ID 1569 Phaeosphaeriaceae Paraphoma radicina CBS 111.79 100 Neophaeosphaeria filamentosa CBS 102203 100 Neophaeosphaeria filamentosa CBS 102202 Neophaeosphaeriaceae Neophaeosphaeria agaves CPC 21264 83 Dothidotthia symphoricarpi CPC 12929 Dothidotthiaceae Dothidotthia aspera CPC 12933 100 Coniothyrium palmarum CBS 758.73 Coniothyriaceae Coniothyrium palmarum CBS 400.71 100 Alternariaster helianthi CBS 134020 Leptosphaeriaceae Alternariaster helianthi CBS 327.69 Leptosphaeria maculans AFTOL-ID 277 75 Pleospora herbarum CBS 191.86 100 Alternaria alternata AFTOL-ID 1610 Pleosporaceae Pyrenophora phaeocomes AFTOL-ID 283 72 Phoma exigua CBS 431.74 97 Didymellaceae Leptosphaerulina australis CBS 317.83 Didymella exigua CBS 183.55 100 Ascocylindrica marina MD6011 Ascocylindrica mari MD6012 97 Corynespora smithii CABI 5649b Corynespora cassiicola CBS 100822 72 Beverwykella pulmonaria CBS 283.53 100 Melanomma pulvis-pyrius CBS 371.75 Melanommataceae Byssosphaeria jamaicana SMH 1403 77 Lindgomyces ingoldianus ATCC 200398 73 Lindgomyces rotundatus KH 114 Lindgomycetaceae 91 Massariosphaeria typhicola MAFF 239218 Lolia aquatic MF644 97 Anguillospora longissimi CCMF 10304 Anguillospora longissima CS869-1D Amniculicola lignicola Ying01 Anguillospora longissima F00980 Amniculicola parva CBS 123092 Amniculicolaceae 100 Spirosphaera cupreorufescens A20 Amniculicola immersa CBS 123083 100 Repetophragma ontariense HKUCC 10830 96 Massariosphaeria grandispora CBS 613 86 70 Pseudomassariosphaeria bromicola MFLUCC 15-0031 Murispora rubicunda IFRD 2017 Hysterium angustatum CBS 236.34 Out group 87 98 0.05 Fig. 49 continued 123 Fungal Diversity (2016) 80:1–270 Saprobic on dead branches. Sexual morph Ascomata immersed, solitary or scattered, coriaceous, globose to subglobose, brown to dark brown. Ostiole central. Peridium comprising light brown cells of textura angularis. Hamathecium comprising cylindrical to filiform, septate, branched, pseudoparaphyses. Asci 8-spored, bitunicate, oblong to cylindrical, short pedicellate, with wide ocular chamber. Ascospores overlapping 1–2-seriate, hyaline, ellipsoid to broadly fusiform, 1-septate, constricted at the septa, surrounded by gelatinous sheath. Asexual morph Undetermined. Type species: Neomassaria fabacearum Mapook, Camporesi & K.D. Hyde, sp. nov. Notes: Neomassaria fabacearum was collected from a dead branch of Hippocrepis emerus (L.) Lassen. Molecular data places Neomassaria in the family Massariaceae with high bootstrap support (91, ML with 0.99, PP) along with other species of Massaria. However, Neomassaria is distinct in morphology of ascomata, asci and ascospores. Phylogenies also reveal that Neomassaria can be considered as distinct genus given that it did not cluster with any Massaria species. Instead it is basal to them with high support (Fig. 43). Neomassaria fabacearum Mapook, Camporesi & K.D. Hyde, sp. nov. Index Fungorum number: IF552274; Facesoffungi number: FoF02438, Fig. 44 Etymology: Name reflects the host family Fabaceae, from which this holotype was collected. Holotype: MFLU 16-1875. Saprobic on a dead branch of Hippocrepis emerus. Sexual morph Ascomata (150–)200–220 lm high 9 (100–)130–150 lm diam. ( x = 190 9 130 lm, n = 5), immersed, solitary or scattered, coriaceous, globose to subglobose, brown to dark brown, Ostiole central. Peridium 10–20 lm wide, comprising light brown cells of textura angularis. Hamathecium comprising 1–2 lm wide, cylindrical to filiform, septate, branched, pseudoparaphyses. Asci 65–75(–85) 9 10–15 lm ( x = 75 9 12 lm, n = 5), 8-spored, bitunicate, oblong to cylindrical, short pedicellate, with wide ocular chamber. Ascospores 18– 20 9 (4–)5–6 lm ( x = 19 9 5 lm, n = 15), overlapping 1–2-seriate, hyaline, ellipsoid to broadly fusiform, 1-septate, constricted at the septum, surrounded by hyaline gelatinous sheath observed clearly when mounted in Indian ink. Asexual morph Undetermined. Material examined: ITALY, Forlı̀-Cesena, CusercoliCivitella di Romagna, on dead branch of Hippocrepis emerus (L.) Lassen (Fabaceae), 11 September 2014, E. Camporesi (MFLU 16-1875, holotype), ex-type culture MFLUCC 14-1117, (isotype as HKAS 95078 in HKAS). 77 Massarinaceae Munk The family Massarinaceae was introduced by Munk (1956) and is typified by Massarina with M. eburnea (Tul. & C. Tul.) Sacc. as the type species. Currently Byssothecium, Corynespora, Helminthosporium, Massarina, Pseudodidymosphaeria, Pseudosplanchnonema, Stagonospora, and Suttonomyces are included in Massarinaceae (Chethana et al. 2015; Tanaka et al. 2015; Thambugala et al. 2015a; Fig. 45). Corynespora leucadendri Quaedvl. et al. and C. olivacea (Wallr.) M.B. Ellis cluster within Massarinaceae, however the generic type of Corynespora, C. cassiicola (Berk. & M.A. Curtis) C.T. Wei clusters in Corynesporaceae, which is a distinct family. Therefore, these two Corynespora species should be renamed. In this study we introduce a new species of Stagonospora. Stagonospora (Sacc.) Sacc. Stagonospora is typified by S. paludosa (Sacc. & Speg.) Sacc., a species from Carex pseudocyparus. Quaedvlieg et al. (2013), reassembled septoria-like genera and introduced Stagonospora sensu stricto in Massarinaceae because of its pycnidial conidiomata, immersed, globose, ostiolate, conidiophores reduced to conidiogenous cells, with holoblastic, percurrent proliferations, and doliiform, cylindrical to ellipsoid, hyaline, guttulate conidia. Tanaka et al. (2015) revised Massarinaceae and included twelve species in Stagonospora. Stagonospora forlicesenensis Phukhamsakda, Camporesi & K.D. Hyde, sp. nov. Index Fungorum number: IF552238; Facesoffungi number: FoF02384, Fig. 46 Etymology: In reference to the location where the fungus was collected. Holotype: MFLU 16-1337 Saprobic on dead branches of Phragmites australis (Cav.) Trin. ex Steud. Sexual morph Undetermined. Asexual morph Conidiomata 70–210 lm high 9 70– 160 lm diam. ( x = 141 9 117 lm, n = 10), pycnidial, solitary, uniloculate, scattered, immersed in the host, dark brown to black, globose, containing basal mycelium, ostiole central. Ostiole 22–72 lm high 9 27–62 lm diam. ( x = 53 9 46 lm, n = 5), papillate, dark brown. Setae 48 9 3 lm, on the side of the pycnidial walls, acicular, curved, constricted at the septate, light brown to brown. Pycnidial wall 8–28 lm (–31 lm at apex), composed of 4– 5 layers of brown-walled cells of textura angularis, two hyaline inner layers, lining bearing conidiogenous cells. Conidiophores reduced to conidiogenous cells with one 123 78 123 Fungal Diversity (2016) 80:1–270 Fungal Diversity (2016) 80:1–270 b Fig. 50 Occultibambusa aquatica (holotype). a Appearance of ascomata on host surface. b Vertical section through ascoma. c Peridium. d–f Asci. g, h Pseudoparaphyses. i, j Ascospores. k Ascospore stained with Indian ink. l Germinating ascospore. m, n Culture characters on PDA (m = from above, n = from below). Scale bars b = 100 lm, c = 40 lm, d, e, l = 20 lm, f = 30 lm, g, h, i–k = 10 lm supporting cell. Conidiogenous cells 4–10 9 2–6 lm, ( x = 7 9 4 lm, n = 30), holoblastic, determinate, discrete, ampulliform to clavate, hyaline, smooth, formed from the inner cells of the pycnidial wall. Conidia 25– 34 9 6–11 lm ( x = 30 9 8 lm, n = 50), broad fusiform to oblong, obtuse at both ends, thick-walled, with minute and large guttules in each cell, 3 transverse eusepta, rarely 2-septate, sometimes slightly constricted at the septa, hyaline; apical appendage, infundibuliform to campanulate-like, gelatinous, 7–8 lm wide. Culture characteristics: Colonies on PDA, reaching 90 mm diam. after 14 days at 16 °C, colonies covering surface, sparse, cream, with abundant aerial mycelium, margins lobate; reverse white at the edges, cream at the center, radiating, circular, flattened, margin rough, not pigmented. Material examined: ITALY, Province of Forlı̀-Cesena, Pian di Spino—Meldola, on dead and stem of Phragmites australis (Poaceae), 22 December 2014, E. Camporesi, IT 2306 (MFLU 16-1337, holotype), isotype in HKAS 94613, ex-type living culture, MFLUCC 15-0054, KUMCC 16-0028. Notes: Based on a morphological comparison, Stagonospora forlicesenensis is similar to Stagonospora (Neottiosporina) paspali G.F. Atk. B. Sutton & Alcorn (CBS 331.37), a species introduced from Paspalum laeve Michaux (Poaceae). In our study, sequence data also reveals a close phylogenetic affinity of Stagonospora forlicesenensis to Stagonospora (Neottiosporina) paspali (Fig. 45). Therefore we compare the morphology with Neottiosporina. Sutton (1980) illustrated seven species of Neottiosporina. Neottiosporina australiensis B. Sutton & Alcorn and N. clavata B. Sutton are also reported from Phragmites australis (Poaceae) (Sutton and Alcorn 1974; Sutton 1980). They are phenotypically similar in having thin pycnidial walls, central ostioles, and holoblastic, determinate, 3-septate, hyaline conidia with infundibuliform apical appendage (Sutton and Alcorn 1974; Sutton 1980). Stagonospora forlicesenensis differs from N. australiensis and N. clavata in having immersed and smaller conidiomata, with brown setae on the pycnidial walls, and conidia which are obtuse at both ends. Therefore Tanaka et al. (2015) suggested these species should be treated under Stagonospora based on morphology and phylogenetic analysis. However, sequence data for the type species are not available and therefore the position remains unconfirmed. 79 Melanommataceae G. Winter Tian et al. (2015) accepted 20 genera in this family based on morphology and phylogenetic analyses. In this paper, we introduce a new species of Bertiella, and an updated phylogenetic tree for the family (Fig. 47). Bertiella (Sacc.) Sacc. & P. Syd. The genus Bertiella is typified by B. macrospora (Sacc.) Sacc. & Traverso. Previously this genus was assigned to Massarina (as M. macrospora (Sacc.) O.E. Erikss. & J.Z. Yue) (Lumbsch and Huhndorf 2010). However, recent phylogenetic studies support its placement within Melanommataceae close to Byssosphaeria (Tian et al. 2015). Taxa are mainly saprobes on woody hosts and characterized by black, superficial, subglobose ascomata with carbonaceous peridium, composed of few layers of pigmented cells (Tian et al. 2015). Bertiella ellipsoidea Ekanayaka, Q. Zhao & K.D. Hyde, sp. nov. Index Fungorum number: IF552201; Facesoffungi number: FoF02229, Fig. 48 Etymology: The specific epithet ellipsoidea is refers to the shape of the ascospores. Holotype: MFLU 16-0583 Saprobic on dead stems. Sexual morph Ascomata 170– 280 9 200–250 lm ( x = 255 9 236 lm, n = 10), arising in small groups, sessile, erumpent from the substrate, subglobose with a flattened base, black, carbonaceous, ostiole area black, ascomata covered with outwardly projecting, long hairs. Peridium 50–60 lm ( x = 56.6 lm, n = 10) wide, outer part composed of thick-walled, strongly melanized cells of textura angularis and inner part filled with elongate, hyaline cells of textura angularis. Hamathecium comprising numerous, 1.3–1.8 lm wide ( x = 1.5 lm, n = 20), long, filiform, aseptate, trabeculate pseudoparaphyses, branching and anastomosing between and above the asci. Asci 112–160 9 10–12 lm ( x = 131 9 11.4 lm, n = 30), 8-spored, bitunicate, cylindric–clavate, short pedicellate, rounded at the apex, with an ocular chamber. Ascospores 14–18 9 6–7 lm ( x = 16.3 9 6.7 lm, n = 40), 1–2 seriate, ellipsoid, greenish, 1-septate, constricted at septum, with 1–2 globules, acute at the apex, smooth, thick-walled. Asexual morph Undetermined. Material examined: THAILAND, Chiang Rai, Mae Fah Luang University, on dead stems, 17 October 2015, A.H. Ekanayaka (MFLU 16-0583, holotype). Notes: Bertiella ellipsoidea is well distinguished by ascomata arising in small groups, sessile, erumpent from the substrate, covered with outwardly projecting, long hairs, trabeculate pseudoparaphyses, branching and 123 80 123 Fungal Diversity (2016) 80:1–270 Fungal Diversity (2016) 80:1–270 b Fig. 51 Occultibambusa chiangraiensis (holotype). a Appearance of ascostromata on host substrate. b, c Close up of ascostromata on host. d Vertical section of ascostroma. e Section through peridium. f Cellular pseudoparaphyses. g–i Developmental stages of asci. j– o Developmental stages of ascospores. p Germinated ascospore. q, r Culture characters. Scale bars b, c = 500 lm, d = 100 lm, e = 50 lm, f–i = 20 lm, j–p = 10 lm anastomosing between and above the asci, bitunicate, 8-spored, cylindric-clavate, asci with an ocular chamber and (14–18 9 6–7 lm) ellipsoid, 1–2 seriate, greenish, 1-septate ascospores. Sequence data obtained directly from the type material clustered within the family Melanommataceae, close to the type species B. macrospora (Sacc.) Sacc. & Traverso (Fig. 47). Bertiella ellipsoidea has ellipsoid ascospores, while in other species in the genus they are fusiform. Furthermore, B. ellipsoidea has greenish, 1-septate ascospores, while in B. macrospora ascospores are brownish and 3-septate. Byssosphaeria villosa (Samuels & E. Müll.) Boise is similar to Bertiella ellipsoidea but differs in having ascospores with a gelatinous sheath (Mugambi and Huhndorf 2009). Occultabambusaceae Dai et al. Dai et al. (2016) introduced Occultabambusaceae to accommodate two pleomorphic genera Occultibambusa and Seriascoma, a coelomycetous genus Versicolorisporium and Neooccultibambusa. In their study, the morphology of sexual morphs and asexual morphs were discussed. In this paper, we introduce two new species, Occultibambusa aquatica and O. chiangraiensis and provide an updated tree (Fig. 49). Occultibambusa aquatica Huang Zhang & K.D. Hyde, sp. nov. Index Fungorum number: IF552366; Facesoffungi number: FoF02439, Fig. 50 Etymology: Refers to its aquatic habitat. Holotype: MFLU 11-1141. Saprobic on submerged wood in freshwater stream. Sexual morph Ascomata perithecioid, 100–250 lm high, 180–280 lm diam., scattered or in small groups, immersed to semi-immersed, subglobose, with a flattened base, brown to dark brown, with minute ostiolate, central papilla, with rounded slot. Papilla up to 40 lm long, 50 lm diam., dark brown. Peridium carbonaceous and fragile, unequal in thickness, 35–45 lm thick at the sides, 25–35 lm thick at the base and near ostiole, composed of 5–7 9 1.5–2.5 lm, rectangular to polygonal celadon cells. Hamathecium comprises 2–3 lm wide, septate, hypha-like, numerous, branched, anastomosing, pseudoparaphyses embedded in mucilage. Asci 73–86 9 9–13 lm ( x = 78.2 9 11.1 lm, 81 n = 10), 8-spored, bitunicate, fissitunicate, clavate, with a short furcate pedicel (5–8 lm), which elongates after discharge (up to 55 lm), apically rounded with a small ocular chamber (1–3 9 4–4.5 lm). Ascospores 19–25 9 3.5– 6.5 lm ( x = 22.2 9 5 lm, n = 20), L/W 3.8–4.8 (mostly 4.5), mostly 2-seriate, narrowly fusiform with acute ends, 1-septate, not constricted at the septum, septum mostly median, upper cell slightly broader than lower cell, slightly swollen near the septum, straight to curved, brownish, with one large guttule in each cell, smooth-walled, thin-walled, surrounded by a 5–10 lm thick sheath. Germ tube mainly formed from both end cells. Asexual morph Undetermined. Culture characteristics: Ascospores germinating on WA within 12–24 h. Colonies on PDA, dense, dark grey, reaching up to 1 cm diameter at after 13 days at 25–28 °C, raised, central embossing, velvety, aerial mycelium and entire edge smooth, clear. Material examined: THAILAND, Chiang Rai, Hui Kang Pla Waterfall, on submerged bamboo, 16 November 2010, Huang Zhang a50 (MFLU 11-1141, holotype), ex-type living culture, MFLUCC 11-0006. Notes: Occultibambusa aquatica is similar to O. bambusae in having clavate asci and 1-septate, fusiform, brown ascospores with a mucilaginous sheath. However, O. bambusae has larger ascomata (400–550 lm diam.) and larger ascospores (23.5–27.5 9 4.5–7 lm). A close phylogenetic relatedness is noted between O. aquatica and O. pustula in a monophyletic subclade basal to other Occultibambusa species (Fig. 49) (99 % MLBP, PP greater than 0.90). Occultibambusa aquatica has no statistical support to O. pustula and is similar in having fusiform, 1-septate ascospores, but the latter has raised and darker ascomata and hyaline to pale brown ascospores. Based on morphological characters and multi-gene phylogenetic analyses, we introduce a novel species in the genus Occultibambusa. Occultibambusa chiangraiensis Phukhamsakda & K.D. Hyde, sp. nov. Index Fungorum number: IF552240; Facesoffungi number: FoF02382, Fig. 51 Etymology: The epithet ‘‘chiangraiensis’’ refers to Chiang Rai Province where the holotype was collected. Holotype: MFLU 16-1334 Saprobic on dead stem of Bambusoideae. Sexual morph Ascostromata 195–295 lm high 9 352–520 lm diam. ( x = 264 9 460 lm, n = 5), erumpent, solitary, scattered, depressed globose to subglobose, flattened at the base, brown to light brown. Ostioles central, with slit-like opening. Peridium (8–)12–34 lm wide, of unequal thickness, thickened at sides, with an outer layer of 7–9 layers of brown to dark brown, heavily pigment cells, arranged in a textura angularis and textura prismatica, and polygonal at the base. Hamathecium composed of dense, 1.6–4(–4.7 in 123 82 123 Fungal Diversity (2016) 80:1–270 Fungal Diversity (2016) 80:1–270 b Fig. 52 RAxML tree based on analysis of a combined dataset of ITS, LSU and SSU partial sequences. Bootstrap support values for maximum likelihood (ML, black) higher than 50 % and Bayesian posterior probabilities (BYPP, Red) greater than 0.95 are defined as above the nodes. The tree is rooted to Didymella exigua. All type strains are in bold. New stains are given in blue enlarged cells) lm wide (n = 30), transversely septate, branched, cellular pseudoparaphyses. Asci 47–92 9 12– 16 lm ( x = 69 9 14 lm, n = 20), 8-spored, bitunicate, clavate-oblong, with a short pedicel, apically obuse, with an ocular chamber, clearly visible when immature. Ascospores 16–24 9 5–7 lm ( x = 22 9 6 lm, n = 30), overlapping bi-seriate, hyaline when immature, pale brown to red-brown at maturity, fusiform, tapering towards the ends, guttulate in each cell, (1–)3-septate, strongly contricted at the median septum, sometimes the cells above median septum wider, smooth-walled, lacking a mucilaginous sheath. Asexual morph Undetermined. Culture characteristics: Colonies on PDA, reaching 50 mm diam. after 4 weeks at 25 °C, dark brown to black at the edge, surface covered with grey mycelium, rarely with aerial mycelium, margins lobate, reverse black radiating, dense, umbonate, margin uneven, producing reddish pigment in agar. Material examined: Thailand, Chiang Rai Province, on dead stem of Bambusoideae sp. (Poaceae), 16 April 2015, C. Phukhamsakda, CP012 (MFLU 16-1334, holotype), extype living culture, MFLUCC 16-0380, KUMCC 16-0027; ibid. (KUN; HKAS 94617, isotype). Notes: Occultibambusa chiangraiensis is closely related to O. bambusae Dai & K.D. Hyde, the type species of Occultibambusa, based on maximum likelihood analysis (87 % ML). However, in O. chiangraiensis the ascostromata are erumpent, solitary, with a mixing of peridium cell types, while in O. bambusae the ascostromata and peridium cells are only textura angularis. The asci of O. bambusae are oblong-clavate and ascospores 3-septate without a sheath (Dai et al. 2016). Occultibambusa chiangraiensis also shares similarity with O. fusispora Phookamsak et al., but the latter has smaller, solitary ascostromata (135–185 lm high 9 240–275 diam.), a thick-walled peridium (up to 60 lm wide), and narrowly fusiform ascospores, acutely tapering at the ends, and not constricted at the septa (Dai et al. 2016). Doilom et al. (2016) introduced Neooccultibambusa chiangraiensis from Tectona grandis. Even though ascospores are similar, N. chiangraiensis has immersed ascomata, with obvious ostioles, larger asci (128 9 20 lm diam.), and cylindrical to subcylindrical, larger ascospores (37 9 10 lm diam.) surrounded by a mucilaginous sheath. 83 Phaeosphaeriaceae M.E. Barr The family Phaeosphaeriaceae was introduced by Barr (1979) to accommodate Dothideomycete species, mostly on monocotyledons and some dicotyledons (Shoemaker and Babcock 1989; Schoch et al. 2006, 2009; Zhang et al. 2009a, 2012a; de Gruyter et al. 2010; Hyde et al. 2013; Phookamsak et al. 2014; Wijayawardene et al. 2014b; Ariyawansa et al. 2015c). Species in Phaeosphaeriaceae vary in morphological characters and comprise more than 35 sexual and asexual genera (Phookamsak et al. 2014; Wijayawardene et al. 2014a; Ariyawansa et al. 2015c). Species in Phaeosphaeriaceae have often been confused with taxa in Leptosphaeriaceae (Zhang et al. 2012a; Hyde et al. 2013; Phookamsak et al. 2014; Ariyawansa et al. 2015b; Tennakoon et al. 2016). Based on phylogenetic analyses, Phaeosphaeriaceae is a heterogeneous group of taxa with shared similar morphology, but is phylogenetically distinct from species of Leptosphaeriaceae and Phaeosphaeria sensu lato (Zhang et al. 2012b; Hyde et al. 2013; Phookamsak et al. 2014; Ariyawansa et al. 2015b; Liu et al. 2015a; Tennakoon et al. 2016). Therefore, several genera have been introduced to accommodate ambiguous phaeosphaeriaceous taxa (Ariyawansa et al. 2015b; Li et al. 2015; Liu et al. 2015a; Phukhamsakda et al. 2015; Tibpromma et al. 2015; Tennakoon et al. 2016). In this study we introduce the new genera Camarosporioides with a single species, Ca. phragmitis, and Pseudophaeosphaeria, with Ps. Rubi. We also introduce the new species, Chaetosphaeronema achilleae, Dematiopleospora alliariae, De. cirsii, Juncaceicola italica, Leptospora galii, Le. aquatica, Le. thailandica, Muriphaeosphaeria ambrosiae, Neodidymelliopsis ranunculi, Nodulosphaeria italica, Poaceicola arundinis and Wojnowicia italica and provide an updated tree (Fig. 52). Camarosporioides W.J. Li & K.D. Hyde, gen. nov. Index Fungorum number: IF552209; Facesoffungi number: FoF02350 Etymology: Morphologically resembling the genus Camarosporium, but phylogenetically distinct. Saprobic on dead stems of Poaceae. Sexual morph Undetermined. Asexual morph Conidiomata yellowish to brown, separate or aggregated, pycnidial, obpyriform, immersed, unilocular, thick-walled, smooth, ostiolate. Ostiole single, circular, with hyaline periphyses, centrally located, papillate. Periphyses hyaline, hyphae-like, smooth, subcylindrical, with obtuse apex, unbranched, septate. Wall of conidiomata composed of thick-walled, brown cells of textura angularis. Conidiophores reduced to conidiogenous cells. Conidiogenous cells hyaline to pale brown, holoblastic, long lageniform, swollen at the base, discrete, determinate, formed from the cells lining the inner wall of 123 84 123 Fungal Diversity (2016) 80:1–270 Fungal Diversity (2016) 80:1–270 b Fig. 53 Camarosporioides phragmitis (holotype). a Herbarium specimen. b Appearance of black coniodiomata on the host. c Vertical section of conidioma. d Ostiole. e, f Section of peridium. g– j Conidiophores, conidiogenous cells and developing conidia. k, l Germinated conidia. m–p Conidia. q Culture on PDA. Scale bars c = 100 lm, d–e = 50 lm, f, k–1 = 20 lm, g–j, m–p = 5 lm, q = 25 mm the conidioma. Conidia pale brown to brown, finely roughened, ellipsoidal to oval, obtuse at the apex, slightly truncate at base, with primary transverse septa, with a longitudinal septum, constricted at septa, verruculose, thick-walled. Type species: Camarosporioides phragmitis W.J. Li & K.D. Hyde, sp. nov. Notes: Combined rDNA gene sequence data reveals that our new genus belongs to the Phaeosphaeriaceae and in particular is close to Stagnospora neglecta and S. foliicola (Fig. 52). Morphologically, Camarosporioides shares similar conidial morphology with Phragmocamarosporium (Massarinaceae), but Phragmocamarosporium lack periphyses. In addition, both genera can be distinguished by the form of conidiomata. Camarosporioides species have yellowish to brown, obpyriform, immersed conidiomata, while Phragmocamarosporium species have black, globose to subglobose conidiomata (Wijayawardene et al. 2016). In combining both morphology and phylogeny, we introduce Camarosporioides as a new genus. Camarosporioides phragmitis W.J. Li & K.D. Hyde, sp. nov. Index Fungorum number: IF552210; Facesoffungi number: FoF02351, Fig. 53 Etymology: Named after the host genus Phragmites. Holotype: MFLU 16-1488. Saprobic on dead stem of Phragmites australis (Cav.) Trin. ex Steud. (Poaceae). Sexual morph Undetermined. Asexual morph Coelomycetous. Conidiomata 143–170 lm diam., 160–177 lm high, yellowish to brown, separate or aggregated, pycnidial, obpyriform, immersed, unilocular, thick-walled, smooth, ostiolate. Ostiole 30–70 9 33– 70 lm, single, circular, with hyaline periphyses, centrally located, papillate. Periphyses hyaline, hyphae-like, smooth, subcylindrical, with obtuse apex, septate, unbranched. Wall of conidiomata composed of thick-walled, brown cells of textura angularis. Conidiophores reduced to conidiogenous cells. Conidiogenous cells 3.5–10 lm long 9 3–7 lm wide, hyaline to pale brown, holoblastic, long lageniform, swollen at the base, discrete, determinate, formed from the cells lining the inner wall of the pycnidium. Conidia 11–18 9 5– 8 lm ( x = 14 9 6 lm, n = 30), pale brown to brown, finely roughened, ellipsoidal to oval, obtuse at the apex, slightly truncate at base, with 1–3 primary transverse septa, 85 and occasionally 1–2 longitudinal septum, constricted at septa, verruculose, thick-walled. Culture characteristics: Colony on PDA, reaching 40–50 mm diam. in 7 days, with circular margin, white and fluffy, dense, aerial mycelium on the surface, reverse similar in colour. Material examined: GERMANY, wet meadow, on dead stem of Phragmites australis (Poaceae), 6 April 2012, René K. Schumacher, G3 (MFLU 16-1488, holotype); extype living culture, MFLUCC 13-0365 (HKAS 95025, isotype); living culture, KUMCC 15-0599. Chaetosphaeronema Moesz The genus Chaetosphaeronema was introduced by Moesz (1915) and is represented by C. hispidulum (Corda) Moesz (type) and C. herbarum (Hollós) Moesz. In this genus the conidiomata are pycnidial and globose and have setae. De Gruyter et al. (2009, 2010) used 18S rDNA and 28S rDNA data to show that Chaetosphaeronema was related to Phaeosphaeriaceae and Pleosporaceae and treated Chaetosphaeronema sensu stricto in Phaeosphaeriaceae. Petrak (1944) and Zhang et al. (2009b) suspected that Chaetosphaeronema may be asexual morph of Ophiobolus. Phookamsak et al. (2014) confirmed the taxonomic position of Chaetosphaeronema in Phaeosphaeriaceae. Chaetosphaeronema achilleae S.K. Huang & K.D. Hyde, sp. nov. Index Fungorum number: IF552176; Facesoffungi number: FoF02240, Fig. 54 Etymology: Named after the host genus Achillea. Holotype: MFU 15-1922. Saprobic on dead cone of Achillea nobilis L. Sexual morph Undetermined. Asexual morph Conidiomata 200– 290 lm high 9 180–245 wide lm ( x = 240 9 200 lm, n = 5) diam., pycnidial, solitary, uniloculate, immersed, subglobose, brown to dark brown, with minute papilla. Ostiole central, short, lined with periphyses. Wall of conidiomata 25–35 lm, membranaceous, composed of dark brown, or brown to hyaline cells of textura angularis. Conidiophores reduced to conidiogenous cells. Conidiogenous cells 7–12 9 1–2 lm ( x = 10 9 1.5 lm, n = 20), enteroblastic, phialidic, cylindrical to subcylindrical, smooth-walled, hyaline, arising from the inner layers of conidioma. Conidia 9–16 9 1.5–3 lm ( x = 12 9 2 lm, n = 50), cylindrical to subcylindrical, slightly curved, hyaline, aseptate to 1-septate, smooth-walled. Culture characteristics: Colonies on PDA reaching 10 mm diam. after 2 weeks at 16 °C, white at the margin, dark brown to green at the center; reverse cream to white at the margins, dark brown to black at the center, curled, circular, umbonate, without diffusible pigments. 123 86 123 Fungal Diversity (2016) 80:1–270 Fungal Diversity (2016) 80:1–270 b Fig. 54 Chaetosphaeronema achilleae (holotype). a Herbarium specimen. b Conidiomata on host. c Immersed conidioma. d Conidioma in vertical section. e Wall of conidioma. f, g Conidiophores and conidia. h–j Conidia. k, l Culture characters on PDA. Notes g stained in lactophenol cotton blue. Scale bars b = 500 lm, c = 200 lm, d = 100 lm, e = 50 lm, f–g = 10 lm, h–j = 2 lm Material examined: RUSSIA, Rostov Region, Oktyabrsky District, southern outskirts of Persianovky settlement, Khoruli gully (rus. balka Khoruli), on dead stems of Achillea nobilis (Asteraceae), 28 April 2015, T.S. Bulgakov (MFLU 15-1922, holotype, GZU 16022408, isotype), ex-type living culture, MFLUCC 16-0476, GZUCC 16022408. 87 Notes: Chaetosphaeronema achilleae is morphologically similar to C. hispidulum (type species of Chaetosphaeronema) in having globose pycnidial, cylindrical conidiogenous cells, and cylindrical to subcylindrical, slightly curved, and hyaline conidia. However, C. hispidulum is distinct from C. achilleae in having comparatively large conidiomata (up to 450 lm diam.) with numerous setae, and a pycnidial wall comprising cells of textura prismatica, while C. achilleae has smaller conidiomata, lacking setae and the pycnidial wall comprises cells of textura angularis (Sutton 1980). Phylogenetic data also indicate C. achilleae is closely to C. hispidulum, but morphological differences stated above support our establishment of a new species (Fig. 52). Fig. 55 Dematiopleospora alliariae (holotype). a Appearance of ascomata on host surface. b, c Vertical sections through ascomata. d Peridium. e Pseudoparaphyses. f, g Asci. h Ascospores. i Germinating ascospore. Scale bars b, c = 100 lm, d–h = 50 lm, i = 25 lm 123 88 123 Fungal Diversity (2016) 80:1–270 Fungal Diversity (2016) 80:1–270 89 Dematiopleospora Wanasinghe et al. Wanasinghe et al. (2014) introduced Dematiopleospora as a monotypic genus in the family Phaeosphaeriaceae to accommodate D. mariae Wanasinghe et al. Subsequently, a second species D. luzulae Wanasinghe et al., was added by Ariyawansa et al. (2015a). The genus is characterized by thick, brown, periphyses in the ostiole, immersed to superficial ascomata and yellowish-brown to brown, muriform ascospores with light end cells. Two new additions are proposed in the genus. Colonies on PDA, reaching 10–12 mm diam. after 7 days at 25 °C, surface dirty white to buff, spreading with moderate aerial mycelium, and even, smooth and entire margins. Material examined: ITALY, Forlı̀-Cesena [FC] Province, Ridracoli—Bagno di Romagna, on dead stem of Alliaria petiolata (M. Bieb.) Cavara & Grande (Brassicaceae), 18 November 2012, Erio Camporesi IT 915 (MFLU 15-0545, holotype); ex-type living culture, MFLUCC 13-0070, ICMP. Notes: Dematiopleospora alliariae is introduced as the third species of Dematiopleospora. Dematiopleospora alliariae mainly differs from the other two species in having ascomata with a crest-like ostiole and uni-seriate ascospores with 3(–4) transverse septa and 2–4 vertical septa. There is also support herein from our molecular data analysis that D. alliariae warrants new species status as it did not cluster with other Dematiopleospora species (Fig. 52). Dematiopleospora alliariae Thambugala, Camporesi & K.D. Hyde, sp. nov. Index Fungorum Number: IF552265; Facesoffungi number: FoF02440, Fig. 55 Etymology: The specific epithet alliariae refers to the host genus on which the fungus occurs. Holotype: MFLU 15-0545. Saprobic on Alliaria petiolata (M. Bieb.) Cavara & Grande. Sexual morph Ascomata 210–350 lm high 9 175–300 lm diam. ( x = 270 9 224 lm, n = 6), solitary to gregarious, scattered, immersed, slightly erumpent, coriaceous, dark brown to black, subglobose to pyriform, thickened at the apex, ostiolate. Ostiole central, papillate, with a pore-like opening. Peridium up to 60 lm wide, thin at the sides, broad at the apex, comprising two strata, outer stratum composed of small, brown to dark brown, somewhat flattened, thick-walled cells of textura angularis, fusing and indistinguishable from the host tissues, inner stratum composed of few layers of lightly pigmented to hyaline cells of textura angularis. Hamathecium comprising 1–2 lm wide, septate, cellular pseudoparaphyses, embedded in a gelatinous matrix. Asci 100– 125 9 10–12 lm ( x = 110 9 10.9 lm, n = 15), 8spored, bitunicate, fissitunicate, cylindrical, pedicellate, rounded at the apex, with a distinct ocular chamber. Ascospores 14–17.5 9 6.4–8.4 lm ( x = 15.5 9 7.5 lm, n = 35), uniseriate, partially overlapping, initially hyaline, becoming yellowish-brown at maturity, ellipsoidal to fusiform, muriform, transversely 3-septate, with 0–3(–4) vertical septa, slightly curved, deeply constricted at the central septum, rounded at the ends, smooth-walled, lacking a mucilaginous sheath. Asexual morph Undetermined. Culture characteristics: Ascospores germinating on PDA within 24 h and producing germ tubes from several cells. Dematiopleospora cirsii Wanasinghe, Camporesi, E.B.G. Jones & K.D. Hyde, sp. nov. Index Fungorum number: IF552135; Facesoffungi number: FoF02205, Fig. 56 Etymology: Name reflects the host genus Cirsium, from which the species was collected. Holotype: MFLU 16-0145. Saprobic on dead and hanging branches of Cirsium sp. Sexual morph Ascomata 250–300 lm high 250–350 lm diam. ( x = 271.1 9 299.5 lm, n = 5), immersed to semierumpent, solitary, scattered, broadly oblong and flattened, dark brown to black, coriaceous, cupulate when dry, ostiolate. Ostiole 50–65 lm high 40–50 lm diam. ( x = 58.2 9 43.4 lm, n = 5), papillate, black, smooth, with short and light brown setae. Peridium 10–20 lm wide at the base, 15–30 lm wide in sides, thick, with 4–5 layers, outer layer heavily pigmented, thick-walled, comprising reddish to dark brown cells of textura angularis, inner layer composed of hyaline thin-walled cells of textura angularis. Hamathecium comprising numerous, 1.5–2 lm wide, filamentous, branched, septate, pseudoparaphyses. Asci 80– 120 9 10–14 lm ( x = 100.9 9 12.3 lm, n = 30), 8spored, bitunicate, fissitunicate, cylindrical to cylindricclavate, pedicellate, thick-walled at the apex, with minute ocular chamber. Ascospores 20–30 9 6–9 lm ( x = 25.9 9 6.6 lm, n = 50), overlapping uni-seriate, muriform, ellipsoidal to subfusiform, slightly curved, upper part wider than the lower part, 6–7 transversely septate, with 1 vertical septum, deeply constricted at the central septum, initially hyaline, becoming golden-brown at maturity, ends remaining cone-shaped, with rounded ends, without a mucilaginous sheath. Asexual morph Undetermined. Culture characteristics: Colonies on MEA, reaching 1.5 cm diam. after 30 days at 18 °C, circular, smooth b Fig. 56 Dematiopleospora cirsii (holotype). a Appearance of asco- mata immersed beneath host substrate. b, c Sections of ascomata. d Close up of ostiole with short, light brown, setose hyphae. e Peridium. f Pseudoparaphyses. g–j Asci. k–n Ascospores. o Germinated ascospore. p, q Culture on PDA (note q reverse). Scale bars a = 500 lm, b = 100 lm, c, d = 50 lm, e, k–o = 10 lm, f = 5 lm, g–j = 20 lm 123 90 margin white at first, greenish-grey after 4 weeks, flat on the surface, without aerial mycelium, reverse blackishgreen. Hyphae septate, branched, hyaline, thin, smoothwalled. Material examined: ITALY, Arezzo [AR] Province, Papiano, dead herbaceous branches of Cirsium sp. Fungal Diversity (2016) 80:1–270 Fig. 58 Leptospora rubella (reference specimen). a Appearance of c ascomata on host. b Close up of an ascoma. c, d Vertical section of ascoma. e Pseudoparaphyses. f, g Asci. h Ascospores. i Germinated ascospore. j Mycelium. k, l Culture on PDA (note l reverse). Scale bars a = 1 mm b = 200 lm, c = 100 lm, d = 50 lm, e = 5 lm, f– j = 20 lm Fig. 57 Juncaceicola italica (holotype). a Appearance on the host substrate. b Section of ascoma. c Section of peridium. d Pseudoparaphyses. e–h Ascus. i–m Ascospores. Scale bars a = 100 lm, b = 20 lm, c = 5 lm, d = 2 lm, e–h = 10 lm, i–m = 5 lm 123 Fungal Diversity (2016) 80:1–270 91 123 92 123 Fungal Diversity (2016) 80:1–270 Fungal Diversity (2016) 80:1–270 b Fig. 59 Leptospora galii (holotype). a, b Appearance of ascomata on host. c Vertical section of ascoma. d, e Peridium. f Pseudoparaphyses. g–i Asci. j–m Ascospores. n Germinating ascospore. o Upper view of culture on PDA. p Lower view of culture on PDA. Scale bars c, g, h, n = 20 lm, d, e, j–m = 10 lm (Asteraceae), 13 June 2013, E. Camporesi (MFLU 16-0145, holotype); (isotype in BBH), ex-type living culture, MFLUCC 13-0615 Notes: In this study, our strain MFLUCC 13-0615 groups with Dematiopleospora mariae (type strain of Dematiopleospora) with very high bootstrap and Bayesian support (Fig. 52). Dematiopleospora cirsii resembles D. mariae in having brown periphyses in the ostiole, a peridium comprising brown to dark brown cells of textura angularis and multi-septate ascospores, but differs in having immersed ascomata, ascospores with 7 transverse septa and much longer lower part than upper part, while D. mariae has superficial ascomata, ascospores with 9–10 transverse septa, and upper and lower parts of equal length. Juncaceicola Tennakoon et al. The genus Juncaceicola (Phaeosphaeriaceae) was introduced by Tennakoon et al. (2016), with J. luzulae as the type species. Juncaceicola italica Tibpromma, Camporesi & K.D. Hyde, sp. nov. Index Fungorum number IF552246; Facesoffungi number: FoF02388, Fig. 57 Etymology: Refers to the name of the country where the holotype was collected Holotype: MFLU 14-0685. Saprobic on dead stem of Dactylis glomerata L. Sexual morph Ascomata 115–130 lm high 9 130–140 lm diam. ( x = 125 9 134 lm, n = 4), superficial, solitary, scattered, globose to subglobose, with a flattened base, easy to remove from the host, shiny, ostiole central, black, smooth-walled. Peridium 5–8 lm, yellow-brown, a single stratum comprised of 2–3 cell layers of textura prismatica. Hamathecium comprising numerous 1.3–3 lm wide, filamentous, guttulate, branched anastomosing, septate pseudoparaphyses. Asci 62–88 9 11–20 lm ( x = 77 9 17 lm, n = 10), 6–8spored, bitunicate, clavate to cylindrical, thin-walled, short pedicellate, apex rounded, ocular chamber not well-developed. Ascospores 18–21 9 6–8 lm ( x = 18 9 7 lm, n = 10), fusiform with broadly to narrowly rounded ends, 3-septate, constricted at septa, enlarged at the second cell, green-yellow, guttulate, surrounded by mucilaginous sheath. Asexual morph Undetermined. Culture characteristics: on PDA reaching 2 cm diam. after 2 weeks at 16 °C, later with dense mycelium, with circular, rough margin, flattened; upper surface white at 93 first, dark brown after 4 weeks, producing pigments in PDA media agar; hyphae septate, branched, thick-walled. Material examined: ITALY, Forlı̀-Cesena Province, Monte Falco, on dead stem of Dactylis glomerata (Poaceae), 15 July 2013, Erio Camporesi, IT1378 (MFLU 14-0685, holotype); ex-type living culture, MFLUCC 13-0750; ibid. (HKAS94558 bis, paratypes). Notes: Phylogenetic results show Juncaceicola italica to be related to J. dactylidis; however, we found our new taxon is well-differentiated from J. dactylidis based on morphological differences. Our new taxon is characterized by superficial ascomata, a peridium of textura prismatica, 6–8-spored asci and 3-septate ascospores, while J. dactylidis has immersed or erumpent ascomata, a peridium of textura angularis, 8-spored asci and 4-septate ascospores (Tennakoon et al. 2016). Leptospora Rabenh. Leptospora is typified by L. rubella (Pers.) Rabenh. Persoon (1801) originally assigned the genus to Sphaeria rubella Pers., however, Rabenhorst (1858) transferred it to Dothideomycetes under Leptospora. Leptospora frequently stains the surface of host tissues red to purple and the red colour is also present at the apical part of ostiolar canal. The taxa has multi-septate ascospores. Leptospora is similar to Ophiobolus Riess base on their ascospore morphology (Shoemaker 1976; Crous et al. 2006). Therefore, in this study we reconstruct the phylogenetic analysis (Fig. 52) for Phaeosphaeriaceae and introduce three new species, L. aquatica, L. galii, L. thailandica with provide a reference specimen for L. rubella (96 %ML, 1.00 PP). Leptospora rubella (Pers.) Rabenh., Klotzschii Herb. Viv. Mycol., Edn 2: no. 532 (1857) Index Fungorum number: IF552325; Facesoffungi number: FoF02442, Fig. 58 Saprobic on decaying wood submerged in freshwater. Sexual morph Ascomata 250–260 lm high, 185–190 lm diam., solitary or aggregated, immersed to semi-immersed, erumpent, globose to subglobose, coriaceous, black, apapillate, ostiolate. Ostiole central, ostiolar canal filled with hyaline to brown cells. Peridium 8–9 lm at base, 20– 24 lm at sides comprising 3–4 layers of pale brown to brown, inwardly lighter, thin-walled cells of textura angularis. Hamathecium comprising numerous, 2–3 lm wide, branched, cellular, hyaline pseudoparaphyses. Asci 100–150 9 5–9 lm ( x = 107 9 6 lm, n = 10), 8-spored, bitunicate, long-cylindrical, short pedicellate, apex rounded, with minute ocular chamber. Ascospores 90– 125 9 2–3 lm ( x = 115 9 2.5 lm, n = 10), arranged spirally in the ascus, initially hyaline becoming pale brown at maturity, filiform, ends narrowly rounded, straight or 123 94 Fig. 60 Leptospora thailandica (holotype). a Specimens. b Appearance of ascomata on host surface. c Vertical section of ascoma. d Ostiolar canal. e Section through peridium. f Cellular 123 Fungal Diversity (2016) 80:1–270 pseudoparaphyses. g–i Developing state of asci. j–l Developing stages of ascospores. m Ascospore stained with Indian ink to show septa. Scale bars c = 100 lm, d, g–m = 50 lm, e, f = 10 lm Fungal Diversity (2016) 80:1–270 95 Fig. 61 Muriphaeosphaeria ambrosiae (holotype). a Herbarium packet. b Herbarium material. c Conidiomata on host. d Close up of conidiomata, superficial on host. e Conidioma in vertical section. f Wall of conidioma. g–i Conidiophores and conidia. j– m Conidia. Notes h–i, m stained in lactophenol cotton blue, g stained in Indian ink. Scale bars c = 1 mm, d = 200 lm, e = 100 lm, f– i = 10 lm, j–m = 5 lm curved, thick-walled, smooth-walled, ascospores are sometimes longer than asci. Asexual morph Undetermined. Culture characteristics: Colonies on PDA 4 cm diam. after 4 weeks at 16 °C, dirty white to creamy at the margins, grey to pale-brown at the center; reverse grey to pale brown and cream at the center, gauzy, curled, flat. Material examined: UK, Hampshire, Swanick Lakes, on decaying wood submerged in freshwater, 28 August 2015, E.B.G. Jones, GJ187 (MFLU 16-0965, reference specimen designated here, HKAS94519), living culture, MFLUCC 16-0122, KUMCC 16-0006. Notes: The strain MFLU 16-0965 has morphologically identical to Leptospora rubella, although, it differs from 123 96 the type of L. rubella as the ostiole lacks a distinct papilla. In the phylogenetic analysis (Fig. 52), our collection grouped with the Leptospora rubella (CPC 11006), thus they are not phylogenetically distinct (Fig. 52). Leptospora galii de Silva & K.D. Hyde, sp. nov. Index Fungorum number: IF552317; Facesoffungi number: FoF02441, Fig. 59 Etymology: Name reflects the host genus Galium. Holotype: HKAS 92493a. Saprobic on dead branch of Galium sp. Sexual morph Ascomata 105–226 lm high, 109–188 lm diam., solitary, Fig. 62 Nodulosphaeria italica (MFLU 16-1359, holotype). a Appearance of ascomata on the host surface. b Section through ascoma. c Section through neck. d Section through peridium. 123 Fungal Diversity (2016) 80:1–270 Fig. 63 Poaceicola arundinis (holotype). a Appearance of ascoma c on host surface. b Vertical section of ascoma. c Ostiole. d Section through peridium. e Cellular pseudoparaphyses. f Immature ascus with ocular chamber. g Mature ascus. h–k Developing state of ascospores. l Ascospore stained with Indian ink to show sheath. m Germinated ascospore. n, o Culture character on PDA from surface and reverse. Scale bars a, b = 100 lm, c, d = 50 lm, e–g, m = 20 lm, h–l = 10 lm scattered to clustered, immersed to semi-immersed to erumpent, globose to subglobose, glabrous, visible as raised, black spots on host surface. Peridium 18–25 lm wide, unevenly thickened, two-layered; inner layer comprising 3–4 cell layers of hyaline cells of textura angularis e Pseudoparaphyses. f, g Asci. h–j Ascospores. Scale bars b = 100 lm, c = 50 lm, d–j = 20 lm Fungal Diversity (2016) 80:1–270 97 123 98 Fungal Diversity (2016) 80:1–270 or 3–4 cell layers of flattened, hyaline cells of textura prismatica; outer layer comprising several layers of brown to dark brown cells of textura angularis. Hamathecium composed of dense, 1.5–2.5 lm wide, filamentous, septate, cellular pseudoparaphyses, anastomosing at the apex. Asci Fig. 65 Wojnowicia italica (holotype). a Herbarium material. b, c c Appearance of ascomata semi-immersed in the host. d–f Hand sections of ascomata showing peridia. g–j Asci with ascospores. k–o Ascospores. p Pseudoparaphyses. q Germination of spore. r Colony on PDA from above. s Colony on PDA from below. Scale bars b–c = 200 lm, d– e = 50 lm, f = 20 lm, g–j, p–q = 10 lm, k–o = 5 lm Fig. 64 Pseudophaeosphaeria rubi (holotype). a, b Appearance of ascomata on host surface. c, d Sections through the ascomata. e Papilla. f Peridium. g Pseudoparaphyses. h Asci with pseudoparaphyses in gelatinous matrix. i–h Asci. m–p Ascospores. Scale bars c, d = 50 lm, e = 20 lm, f, g–l = 20 lm, m–p = 5 lm 123 Fungal Diversity (2016) 80:1–270 99 123 100 Fungal Diversity (2016) 80:1–270 55–87 9 3.7–6.9 lm ( x = 70 9 5.2 lm, n = 30), 8spored, bitunicate, cylindric-clavate, short pedicellate, with an obtuse to slightly furcate end, apically rounded, with ocular chamber when immature and indistinct at maturity. Ascospores 17–32 9 2.1–3.3 lm ( x = 23.2 9 2.7 lm, n = 30), overlapping bi-seriate, hyaline, elongate fusiform with obtuse ends, and sometimes curved at ends, 1-septate at maturity, guttulate. Asexual morph Undetermined. Culture characteristics: Colonies on PDA 32–35 mm diam. after 3 weeks, colonies sparse, circular, flat, surface smooth with entire edge, velvety with smooth aspects, zonate with different sector light brown to brown at the margin whitish-grey at the centre; reverse light brown at margin, brown at the middle, dark brown at the centre. Material examined: ITALY, Forlı̀-Cesena Province, Monte Mirabello-Predappio, on dead branch of Galium sp. (Rubiaceae), 11 May 2015, E. Camporesi, IT2478 (HKAS 92493, holotype), (MFLU 15-1230, isotype); ex-type living culture KUMCC 15-0521, MFLUCC. Notes: Our new species clearly fits into Leptospora where all species constitute a monophyletic clade with high support (Fig. 52). In addition, phylogenies also reveal that L. galli is phylogenetically distinct as it branches basal to other species with high support. Leptospora galii differs from other Leptospora species by having hyaline, elongate, fusiform, rarely light yellow, mature, 1-septate ascospores, 17–32 lm long, while other Leptospora species in the same clade have brown, filiform, multi-septate ascospores more than 60 lm long. Fig. 66 Phylogram generated from maximum likelihood analysis based on combined LSU, ITS and SSU sequence data of Pleosporaceae. Maximum likelihood bootstrap support values greater than 60 % are shown above nodes. The ex-type strains are in bold and the new isolate is in blue. The tree is rooted with Leptosphaeria doliolum 123 Leptospora thailandica Phukhamsakda & K.D. Hyde, sp. nov. Index Fungorum number: IF552239; Facesoffungi number: FoF02381, Fig. 60 Etymology: The name reflects the country, where the holotype was collected. Holotype: MFLU 16-1335 Fungal Diversity (2016) 80:1–270 101 Fig. 67 Comoclathris italica (holotype). a Appearance of ascomata on host substrate. b Section of ascoma. c Section of peridium. d Pseudoparaphyses. e, f Asci. g–i Ascospores. j Germinating ascospore. Scale bars b = 50 lm, c = 5 lm, d = 2 lm, e, f = 20 lm, g–j = 10 lm Saprobic on dead branches of Duranta sp. Sexual morph Ascomata 188–207 lm high 9 112–170 lm wide diam. ( x = 197 9 141 lm, n = 10), immersed to erumpent through host tissue with papilla, solitary, scattered, globose, smooth, brown to dark brown, ostiole central. Ostioles 66–115 lm high 9 53–102 lm diam. 123 102 ( x = 79 9 83 lm, n = 10), papillate, dark brown to light brown, heavily pigmented at outer layer, smooth, filled with periphyses, orange around pore. Peridium (5–)10– 24(–27) lm wide, up to 30 lm wide at the apex, thickwalled, brown to dark brown, pseudoparenchymatous cells, composed of 5–7 layers of textura angularis, in hyaline gelatinous layers, thin. Hamathecium comprising numerous, filamentous, 1.7–4 lm (n = 50) wide, broad, branched, transversely septate, cellular pseudoparaphyses. Asci 68–114 9 7–13 lm ( x = 94 9 10 lm, n = 35), 8-spored, bitunicate, cylindrical to cylindrical-clavate, with short furcate pedicel, apically rounded, ocular chamber visible when immature. Ascospores 63–89 9 1.8–3.8 lm Fig. 68 Maximum Parsimony tree obtained from a heuristic search with 1000 random taxon additions of combined LSU and ITS sequence data using PAUP v. 4.0b10. Bootstrap support values of more than 50 % are indicated above the nodes, and Bayesian posterior probabilities more than 0.9 are indicated in bold. The isolate from this study is shown in blue background, and Biatriospora marina as the out group taxon, and the scale bar shows 10 changes. The extype strains are in bold 123 Fungal Diversity (2016) 80:1–270 Fig. 69 Neoroussoella lenispora (holotype). a, b Appearance of c ascostromata on the host surface. c Pseudoparaphyses. d Vertical section through ascostroma. e Section of peridium. f–i Long cylindrical asci with ascospores. j–m Ascospores. Scale bars a = 500 lm, b = 100 lm, c = 30 lm, d = 50 lm, e = 10 lm, f–i = 20 lm, j– m = 5 lm ( x = 76 9 3 lm, n = 40), fasciculate, scolecosporous, filiform, tapering towards the ends, minute guttules in each cell, hyaline when immature, pale brown at maturity, (14–) 20–22-septate, contricted at the septa. Asexual morph Undetermined. Fungal Diversity (2016) 80:1–270 103 123 104 Culture characteristics: Colonies on PDA, reaching 20 mm diam. after 4 weeks at 16 °C, surface cream, dense, circular, convex with moderate aerial mycelium, downy, slightly irregular at margins; reverse cream at the edges, brown at the center, dense, margin rough, not pigmented. Material examined: THAILAND, Chiang Rai, on dead branches of Duranta sp. (Verbenaceae), 12 June 2015, C. Phukhamsakda, CP014 (MFLU 16-1335, holotype), isotype in HKAS 94616, ex-type living culture, MFLUCC 16-0385, KUMCC 16-0030. Notes: Leptospora thailandica forms a sister clade (99 % ML, 100 %PP) with L. aquatica, L. galii (this study), and L. rubella (Pers.) Rabenh., the type species of Leptospora. Leptospora thailandica is similar to Leptospora in staining the host substrate pinkish-red and having remarkable reddish colour at the apical part of ostiole canal, with pale brown and cylindrical ascospores (Crous et al. 2006). Muriphaeosphaeria C. Phukhamsakda et al. Muriphaeosphaeria was introduced by Phukhamsakda et al. (2015) as a monotypic genus with M. galatellae Phukhamsakda et al. as the type species. The genus is characterized by superficial ascomata, muriform ascospores and conidiomata with cylindrical to subclavate, 1–3- Fig. 70 Phylogram generated from maximum likelihood analysis (RAxML) based on combined ITS and LSU sequence data. Bootstrap support values for maximum likelihood (ML, red) and maximum parsimony (MP, green) equal to or greater than 50 % are given above the nodes. The values of the Bayesian posterior probabilities from 123 Fungal Diversity (2016) 80:1–270 Fig. 71 Neotorula submersa (HKAS 92660, holotype). a Colonies c on the substrate. b–d Branched chains of conidia with conidiophores. e–j Conidia. k Germinating conidium. l, m Cultures on PDA. Scale bars b = 30 lm, c, d = 20 lm, e, h–k = 15 lm, f, g = 10 lm septate and brown conidia. This genus was accepted in Phaeosphaeriaceae based on combined ITS, 18S and 28S sequence data (Fig. 52). Muriphaeosphaeria ambrosiae S.K. Huang & K.D. Hyde, sp. nov. Index Fungorum number: IF552177; Facesoffungi number: FoF02241, Fig. 61 Etymology: The specific epithet ambrosiae is based on the host genus from which the holotype was collected. Holotype: MFU 15-1971 Saprobic on dead branch of Ambrosia artemisiifolia L. Sexual morph Undetermined. Asexual morph Conidiomata 228–328 lm high 9 224–268 wide lm ( x = 281 9 249 lm, n = 5), globose, cleistothecial, solitary, uniloculate, black, superficial, globose to subglobose. Wall of conidiomata 10–14 lm diam., membranaceous, composed of dark brown, or hyaline to brown cells of textura angularis. Conidiophores reduced to conidiogenous cells. Conidiogenous cells 7–12 9 1.5–3 lm ( x = 9 9 2 lm, MCMC analyses (BYPP, blue) equal or higher than 95 % are given below the nodes. The tree was rooted to Melanomma pulvis-pyrius. Newly generated sequences are indicated in red, and other ex-type strains are in bold Fungal Diversity (2016) 80:1–270 105 123 106 n = 20), enteroblastic, phialidic, oblong to cylindrical, smooth-walled, hyaline. Conidia 9.5–11.5 9 3–4 lm ( x = 10 9 3.5 lm, n = 50), oval, fusiform to oblong, 1-septate, initially hyaline, pale brown at maturity, smoothwalled, with three inconspicuous and filiform, apical appendages at the apex. Material examined: RUSSIA, Rostov Region, Shakhty City, Cotton Fabric Urban Microdistrict, Grushevka steppe slopes, near Grushevsky Pond, on dead stems of Ambrosia artemisiifolia (Asteraceae), 12 May 2015, T.S. Bulgakov (MFLU 15-1971, holotype, GZU 2016022413, isotype). Notes: We could not obtain a culture from single conidia. Therefore fungal DNA was extracted directly from the conidiomata. Muriphaeosphaeria ambrosiae is a sister to M. galatellae with moderate support (Fig. 52). Both these species are characterized by globose to subglobose, uniloculate conidiomata, and pycnidial wall comprising cells of textura angularis. They were found on Asteraceae. The asexual morph of M. galatellae is distinct from M. ambrosiae in having cylindrical to subclavate, 1–3septate conidia, while M. ambrosiae has 1-septate, oval to oblong conidia with three inconspicuous appendages at the apex (Phukhamsakda et al. 2015). Nodulosphaeria Rabenh. Nodulosphaeria is a relatively poorly studied genus lacking a modern taxonomic treatment and phylogenetic analyses (Hyde et al. 2013; Phookamsak et al. 2014). Since 2014, mycologists have attempted to resolve its natural placement in Phaeosphaeriaceae by recollecting samples and investigated their phylogeny (Ariyawansa et al. 2015c; Li et al. 2015; Liu et al. 2015a). Presently, there are 28 sequences from seven species available in GenBank, with 64 epithets in Index Fungorum (2016). The most recent treatment of Nodulosphaeria which includes molecular data is that of Mapook et al. (2016) and this is followed here. Nodulosphaeria has relatively large ascomata, and phragmospores or scolecospores, with an enlarged cell in the upper part of the ascospores. Nodulosphaeria differs from Ophiobolus in having brown, setae-like periphyses in the ostiole, and ascospores do not separate into part spores (Phookamsak et al. 2014; Mapook et al. 2016). Many Nodulosphaeria species were transferred to Ophiobolus by Shoemaker (1976, 1984). Subsequent authors introduced new species and designated reference specimens to resolve the placement of Nodulosphaeria (Phookamsak et al. 2014; Ariyawansa et al. 2015a; Li et al. 2015; Liu et al. 2015a; Mapook et al. 2016). However, many species still lack modern taxonomic treatments or molecular data to clarify their placement. Nodulosphaeria italica Phookamsak, Camporesi & K.D. Hyde, sp. nov. 123 Fungal Diversity (2016) 80:1–270 Fig. 72 RAxML phylogenetic tree generated from combined LSU c and ITS sequence data from species of Tubeufiaceae. The final ML optimization likelihood is -16263.541876. Bootstrap support values greater than 50 % are shown at the nodes and Bayesian posterior probabilities greater than 0.95 are marked with an asterisk (*). The tree is rooted with Hysteropatella elliptica (Patellariaceae). Type species are in bold and marked (T) and reference specimens are marked (R) Index Fungorum number: IF552202; Facesoffungi number: FoF02261, Fig. 62 Etymology: The specific epithet ‘‘italica’’ refers to the country, where the holotype was collected. Holotype: MFLU 16-1359 Saprobic on Cirsium sp. Sexual morph Ascomata 240– 330 lm high (excluding necks), 280–330 lm diam., dark brown to black, scattered, gregarious, immersed to erumpent through the host cortex, globose to subglobose, uniloculate, setose, covered by dark brown, septate, vegetative hyphae. Papilla 190–330 lm high, 120–140 lm diam., truncate to cylindrical, composed of several layers of thick, brown to dark brown, pseudoparenchymatous cells, arranged in a textura angularis, ostiole central, pore-like opening, with brown, 2–3-septate, setae-like periphyses. Peridium 16–34 lm wide, thin-walled, of equal thickness, composed of two types of brown to dark brown, pseudoparenchymatous cells, inner layers comprising 2–3 layers, of flattened, dark brown cells, arranged in textura prismatica to textura angularis, outer layers comprising 1–2 layers, of thickened, subhyaline to black cells, arranged in textura angularis to textura globulosa. Hamathecium composed of dense, 2–4 lm wide, cellular pseudoparaphyses, distinctly septate, anastomosing at the apex, embedded in a hyaline gelatinous matrix. Asci (157–)170– 200(–206) 9 (11–)12–14(–15) lm ( x = 183.5 9 13.2 lm, n = 30), 8-spored, bitunicate, fissitunicate, cylindrical, short pedicellate, with obtuse to furcate pedicel, apically rounded, with well-developed ocular chamber. Ascospores (114–)120–150(–158) 9 (2–)3–4(–5) lm ( x = 141.5 9 3.8 lm, n = 30), tri- to tetra-seriate in parallel, not fasciculate, yellowish-brown to brown, filiform, with rounded ends, tapered towards lower cell, enlarged at the 8th cell, multi-septate (17–20 septa), constricted at the central septum, smooth-walled, with guttules. Asexual morph Undetermined. Material examined: ITALY, Province of Trento[TN], Mezzana, Marilleva 900, on dead stem of thistle (Cirsium sp.), 3 June 2014, E. Camporesi, IT 549 (MFLU 16-1359, holotype; MFLU 16-1360, isotype); ibid. 9 June 2014, E. Camporesi (MFLU 16-1361); 16 July 2012, E. Camporesi (MFLU 15-0449). Notes: Nodulosphaeria italica is similar to N. cirsii (P. Karst.) L. Holm in having brown, scolecosporous ascospores with multi-septa. However, they can be distinguished Fungal Diversity (2016) 80:1–270 107 123 108 Fig. 72 continued 123 Fungal Diversity (2016) 80:1–270 Fungal Diversity (2016) 80:1–270 109 Fig. 73 Aquaphila albicans (MFLU 16-1136, reference specimen). a Conidia arise directly from hyphal cells on natural substrate. b– d Conidiophores with attached conidia. e–h Conidia. i Germinating spore. j, k Colonies on MEA from surface and reverse. Scale bars a = 200 lm, b–i = 50 lm, j, k = 20 mm by the size of asci and ascospores, as N. italica has smaller asci and ascospores than N. cirsii. Based on phylogenetic analyses, N. italica sits with N. senecionis in a robust clade (92 % ML, 0.95 PP), while N. scabiosae is basal to both species (Fig. 52). Poaceicola W.J. Li et al. The genus Poaceicola was introduced by Li et al. (2015) to accommodate taxa from Poaceae. The genus includes Po. arundinis Li et al. (type species), Po. bromi Wijayawardene et al. and Po. elongata (Wehm.) 123 110 123 Fungal Diversity (2016) 80:1–270 Fungal Diversity (2016) 80:1–270 b Fig. 74 Chlamydotubeufia helicospora (MFLU 16–1338, holotype). a Conidia arise directly from hyphal cells on natural substrate. b Conidiophores with attached conidia. c Conidiogenous cells. d Germinating conidium. e–h Conidia. i–j Colonies on MEA from above and below. Scale bars a = 500 lm, b, e–h = 50 lm, c = 20 lm, d = 100 lm, i–j = 20 mm Shoemaker & C.E. Babc.) Li et al. Poaceicola is characterized by immersed to semi-immersed ascomata, subepidermal, globose, papillate, usually reddish-brown and fusiform ascospores (Li et al. 2015). Specimens in the genera produce brown conidia and are coelomycetous. Poaceicola elongata has a sexual morph which is phaeosphaeria-like (Ariyawansa et al. 2014b). Li et al. (2015) introduced the asexual morph into Phaeosphaeriaceae based on combined LSU and ITS sequence data. From the analysis they found Poaceicola is closely related to P. elongata (96 % ML), and therefore they synonymized Phaeosphaeria elongata under Poaceicola elongata. In this study, we reconstruct the phylogeny of genera of Phaeosphaeriaceae and our strain (MFLUCC 14-1060) clusters together with Po. arundinis (MFLUCC 15-0702) with high support. We therefore describe the sexual morph for Po. arundinis. Poaceicola arundinis W.J. Li, Camporesi, D.J. Bhat & K.D. Hyde, Mycosphere 6 (6): 681 (2015) Facesoffungi number: FoF02383, Fig. 63 Saprobic on dead stem of Dactylis sp. Sexual morph Ascomata 164–230 lm high 9 210–290 lm wide diam. ( x = 200 9 235 lm, n = 10), superficial, solitary, scattered, or sometimes gregarious, globose, black to dark brown, ostiole central. Ostioles 70–85 lm high 9 87– 110 lm diam. ( x = 80 9 100 lm, n = 5), papillate, dark brown, smooth, without periphyses. Peridium 21–35 lm wide., up to 45 lm wide at the apex, composed of 6–7 layers of textura angularis, outer region heavily pigment, cells 4– 8 lm wide, inner layer composed of hyaline gelatinous cells, thin, merging with pseudoparaphyses. Hamathecium comprising numerous, long, 2–3 lm (n = 40) wide, transversely septate, branched, cellular pseudoparaphyses. Asci 65– 105 9 8–19 lm ( x = 87 9 13 lm, n = 20), 7–8-spored, bitunicate, cylindric-clavate, with short bulbose pedicel, apically rounded, with an ocular chamber up to 1–2 lm wide 9 1–2 lm high. Ascospores 23–34 9 4.5–8 lm ( x = 29 9 6 lm, n = 30), bi-seriate or overlapping, hyaline when immature, pale brown to yellowish at maturity, fusiform, tapering towards the ends, (5–)8–9-distoseptate, slightly contricted at third septa, third cell from apex enlarged, smooth-walled, surrounded by thick, distinct mucilaginous sheath. Asexual morph Coelomycetous. Conidiomata 100–150 lm high, 100–200 lm diam., pycnidioid, dark brown, solitary or aggregated, semi-immersed, 111 unilocular, globose, papillate. Pycnidial wall comprised of cells of textura angularis, gradually merging with the outer, surrounding layers of brown, textura oblita. Ostiole central. Conidiophores reduced to conidiogenous cells. Conidiogenous cells enteroblastic, phialidic, hyaline, smooth-walled, discrete. Conidia 30–40 9 6.5–10 lm, pale brown, cylindrical, flexuous, up to 8-euseptate, slightly curved, smooth-walled, with middle cells wider than end cells, guttulate, with an acute apex, truncate at the base (Li et al. 2015). Culture characteristics: Colonies on PDA reaching 43 mm diam. after 14 days at 16 °C, surface white, with moderate aerial mycelium, fluffy, fimbriate margins; reverse white at the edges, dark green at the center, radiating light green outwardly, dense, circular, flattened, margin rough, not pigmented. Material examined: ITALY, Trento [TN], Forte Strino—Vermiglio, on dead stem of Dactylis sp. (Poaceae), 12 September 2014, E. Camporesi, IT 541 (MFLU 16-1336); KUN HKAS 94614, living culture, MFLUCC 14-1060, KUMCC 16-0029. Notes: Based on the combined gene analysis, Poaceicola arundinis (MFLUCC 14-1060) has a close relationship with P. arundinis (MFLUCC 15-0702), reported from Arundo plinii (98 % ML, 100 % PP support). Poaceicola arundinis (MFLUCC 14-1060) is similar to P. elongata, but relatively smaller than the epitype (Ariyawansa et al. 2014b). Li et al. (2015) introduced P. arundinis from the asexual morph and the phylogeny support them to be the same strain as our sexual morph. We therefore introduce the sexual morph to accommodate P. arundinis. Pseudophaeosphaeria Jayasiri, Camporesi & K.D. Hyde, gen. nov. Index Fungorum number: IF552207; Facesoffungi number: FoF02345 Etymology: Named because of its morphological similarity to the genus Phaeosphaeria. Saprobic on Rubus idaeus L. Sexual morph Ascomata scattered, immersed to slightly erumpent through host tissue, visible as small irregular black spots on the host surface, uniloculate, globose to subglobose, glabrous, dark brown, ostiole central, with a minute papilla. Peridium wide, thin-walled, of equal thickness, composed of 3–5 layers of flattened cells, outer layer dark brown, inner layer of pseudoparenchymatous cells, arranged in textura angularis. Hamathecium composed of numerous, wide, filiform, distinctly septate, frequently anastomosing, narrow, cellular pseudoparaphyses, embedded in mucilaginous matrix between and above asci. Asci 8-spored, bitunicate, fissitunicate, broadly cylindrical, subsessile with minute knoblike pedicel, apically rounded, with a well-developed ocular chamber. Ascospores overlapping 1–3-seriate, hyaline, phragmosporous, narrowly fusiform, mostly 3-septate, 123 112 indistinctly constricted at the septa, slightly curved, smooth-walled. Asexual morph Undetermined. Type species: Pseudophaeosphaeria rubi Jayasiri, Camporesi & K.D. Hyde Notes: Phylogenetic analyses of combined LSU, SSU and ITS sequence data indicate that Pseudophaeosphaeria is a distinct genus in Phaeosphaeriaceae, which forms a clade sister to Paraphoma, Setomelanomma and Xenoseptoria clades. Pseudophaeosphaeria, differs from Setomelanomma in having irregular ascomata and narrowly fusiform ascospores, while Setomelanomma has regular ascomata and ellipsoidal to broadly fusiform ascospores. Paraphoma and Xenoseptoria are asexual genera of this family. Pseudophaeosphaeria is morphologically most closely related to Phaeosphaeria sensu stricto, which has immersed to slightly erumpent, irregular, black ascomata, broadly cylindrical asci with small subsessile knob-like pedicels, and narrowly fusiform, 3-septate ascospores. However, this relationship is not supported by sequence data, as Pseudophaeosphaeria is phylogenetic distinct from Phaeosphaeria sensu stricto (Fig. 52). Pseudophaeosphaeria rubi stands phylogenetically apart other genera with relatively high support (Fig. 52). Pseudophaeosphaeria rubi Jayasiri, Camporesi & K.D. Hyde, sp. nov. Index Fungorum Number: IF552174; Facesoffungi number: FoF02221, Fig. 64 Etymology: The specific epithet rubi is based on the host genus from which the taxon was collected. Holotype: MFLU 15-1400 Saprobic on Rubus idaeus L. Sexual morph Ascomata 105–145 lm high, 125–135 lm diam., scattered, immersed to slightly erumpent through host tissue, visible as small irregular black spots on the host surface, uniloculate, globose to subglobose, glabrous, dark brown, ostiole central, with a minute papilla. Peridium 12–18 lm wide, thinwalled, of equal thickness, composed of 3–5 layers of flattened cells, outer layer dark brown, inner layer pseudoparenchymatous cells, arranged in textura angularis. Hamathecium composed of numerous, 1.8–2.4 lm wide, filiform, distinctly septate, frequently anastomosing, narrow cellular pseudoparaphyses, embedded in mucilaginous matrix between and above asci. Asci 59–75 9 7–9 lm ( x = 66.5 9 8.2 lm, n = 20), 8-spored, bitunicate, fissitunicate, broadly cylindrical, subsessile with minute knoblike pedicel, apically rounded, with a well-developed ocular chamber. Ascospores 17–21 9 2.3–3.9 lm ( x = 18.6 9 2.8 lm, n = 30), overlapping 1–3-seriate, hyaline, phragmosporous, narrowly fusiform, mostly 123 Fungal Diversity (2016) 80:1–270 Fig. 75 Helicoma guttulatum (holotype). a Conidiophores with c attached apical conidium on natural substrate. b, c Conidiophores with conidia at the apex. d Conidiogenous cell with conidiophores. e Germinating conidium. f–h Conidia. i–j Colonies on MEA from above and below. Scale bars a = 200 lm, b, c, e–h = 20 lm, d = 10 lm, i–j = 20 mm 3-septate, slightly constricted at the septa, slightly curved, smooth-walled. Asexual morph Undetermined. Culture characteristics: Ascospores germinating on MEA within 36 h. Colonies on MEA, reaching 2.2 cm diam. in 1 week at 28 °C. Mycelium superficial, felty, gummy, pale grey to black. No asexual morph formed in culture. Material examined: ITALY, Forlı̀-Cesena, Passo la Calla—Santa Sofia, on dead branch of Rubus idaeus (Rosaceae), 28 January 2014, Erio Camporesi IT 1680 (MFLU 15-1400, holotype), (isotype in KUN), ex-type culture MFLUCC 14-0259, KUMCC. Wojnowicia Sacc. The genus Wojnowicia was introduced by Saccardo (1892) to accommodate W. hirta. The sexual morph of Wojnowicia has not been reported. Presently, there are 14 species epithets in Wojnowicia (Index Fungorum 2016) which are coelomycetous fungi. However, W. graminis (McAlpine) Sacc. & D. Sacc. and W. tenella Pat. have been synonymized under W. hirta (Sutton 1980) and W. buxi Bertault & Malençon under W. ephedrae Hollós (Farr and Bills 1995). Wojnowicia bryophila Racov., W. exilis (Corda) Sacc. & Traverso, W. viburni Wijayaw. et al. and W. lophostoma (Höhn.) Sacc. were excluded from Wojnowicia (Sutton 1980; Farr and Bills 1995; Wijayawardene et al. 2013; Crous et al. 2015a). Li et al. (2016) introduced and illustrated W. spartii based on morphological characters and phylogenetic analysis. In the present study, the sexual morph of Wojnowicia is described and eight species are accepted in the genus. Wojnowicia italica Qing Tian, Camporesi & K.D. Hyde, sp. nov. Index Fungorum number: IF552183; Facesoffungi number: FoF02254, Fig. 65 Etymology: In reference to the occurrence of this species in Italy. Holotype: MFLU 14-0732. Colonies growing on a dead branch of Spartium junceum L. Sexual morph Ascomata 230–260 9 130– 165 lm ( x = 240 9 150 lm, n = 7), immersed or semiimmersed and erumpent in the host, solitary, scattered, sometimes gregarious, subglobose to globose, black, up to Fungal Diversity (2016) 80:1–270 113 123 114 Fungal Diversity (2016) 80:1–270 Fig. 76 Neoacanthostigma septoconstrictum (MFLU 16-1134). a Conidia arise directly from hyphal cells on natural substrate. b Conidiophores with conidia. c–e Conidia. f Germinating spore. g, h Colonies on MEA from above and below. Scale bars a = 200 lm, b–e = 100 lm, f = 50 lm, g–h = 30 mm 200 lm high. Ostiole inconspicuous. Peridium thick at the apex 30–60.5 lm ( x = 42 lm, n = 7), thinner at the base and sides 17–30 lm ( x = 24 lm, n = 7), one-layered, composed of brown to black, thin-walled, cells of textura angularis. Asci 60–93 9 15–20 lm ( x = 77 9 16 lm, n = 10), 8-spored, bitunicate, clavate, short-pedicellate, 123 Fungal Diversity (2016) 80:1–270 115 Fig. 77 Tubeufia hyalospora (holotype). a Conidia arise directly from hyphal cells on natural substrate. b, c Conidiophores with conidia. d–f Conidia. g Germinating spore. h, i Colonies on MEA from surface and reverse. Scale bars a = 100 lm, b, c = 50 lm, d– g = 20 lm, h, i = 30 mm apically rounded, with an inconspicuous ocular chamber. Hamathecium consisting of 2–3 lm broad long, colourless, branched, pseudoparaphyses, with transverse sept. Ascospores 18–27 9 5–8 lm ( x = 22 9 8 lm, n = 10), overlapping uni-seriate or bi-seriate, light brown to brown, ellipsoid to fusiform, slighty curved, muriform, with 4–7 transverse septa, and one longitudinal septum in some central cells, constricted at the septa, end cells conical, smooth-walled, lacking a mucilaginous sheath. Asexual morphs Coelomycetous. Conidiomata pycnidial, dark brown, scattered, immersed to semi-immersed, globose to subglobose, glabrous, ostiolate. Ostiole centrally located, papillate. Wall of conidiomata composed of 4–5-cell layers. Conidiogenous cells phialidic, hyaline, integrated, flask-shaped, thick-walled. Conidia dark brown, fusiform or cylindrical, 7–12-septate. 123 116 Fungal Diversity (2016) 80:1–270 Culture characteristics: Ascospore germinating on PDA within 12 h. Colonies on PDA reaching 10 mm diam. in 7 days at 16 °C. Mycelium superficial, initially white, later becoming light brown or grey, hairy, with entire edge. Material examined: ITALY, Forlı̀-Cesena [FC] Province, Teodorano-Meldola, on dead stem of Spartium junceum (Fabaceae), 15 December 2012, Erio Camporesi (MFLU 14-0732, holotype), (HKAS 94522, isotype); extype living cultures, MFLUCC 13-0447, KUMCC 16-0017. Notes: The sexual morph of Wojnowicia had not been previously reported. However, we found the sexual morph of W. italica. Its placement in Wojnowicia is supported by Fig. 78 Tubeufia roseohelicospora (holotype). a Conidia arise directly from hyphal cells on natural substrate. b, c Conidiophores with conidia. d–f Conidia. g Germinating spore. h, i Colonies on MEA from above and below. Scale bars a = 200 lm, b–f = 20 lm, g = 50 lm, h, i = 20 mm 123 Fungal Diversity (2016) 80:1–270 117 Fig. 79 Phylogram generated from maximum likelihood analysis based on combined LSU and SSU sequence data. Maximum likelihood bootstrap values greater than 50 % are shown above the branches. The ex-type strains are in bold and the new isolate is in blue. The tree is rooted with Gloniopsis praelonga phylogenetic analyses of combined ITS, LSU and SSU sequence data. Comoclathris italica Tibpromma, Camporesi & K.D. Hyde, sp. nov. Index Fungorum number IF552245; Facesoffungi number: FoF02387, Fig. 67 Etymology: refers to the name of the country where the holotype was collected. Holotype: MFLU 15-1491. Saprobic on dead stem of Thalictrum sp. Sexual morph Ascomata 195–215 lm high 9 225–250 lm diam. ( x = 203 9 230 lm, n = 5), globose to subglobose, solitary, dark brown to black, immersed, black, smooth, ostiolar canal filled with sparse periphyses. Peridium 6–27 lm wide, comprising 3–4 layers of brown to reddish-brown Pleosporaceae Nitschke The family Pleosporaceae was introduced by Nitschke (1869) and Pleospora is the type genus of this family. Pleosporaceae is the largest family in the Pleosporales and is representative of the order (Zhang et al. 2012b; Hyde et al. 2013; Wijayawardene et al. 2014b). A backbone tree for Pleosporaceae was provided by Ariyawansa et al. (2015c) and this is updated in Fig. 66. Taxa of this family are pathogenic or saprobic on wood and dead herbaceous stems or leaves (Ariyawansa et al. 2015c). 123 118 123 Fungal Diversity (2016) 80:1–270 Fungal Diversity (2016) 80:1–270 b Fig. 80 Pleurotrema thailandica (holotype). a Appearance of ascomata on host surface. b Close up of clypeus of ascomata. c, d Vertical sections through ascomata. e Peridium. f–i Asci. j Apical thickening. k Pseudoparaphyses. l–o Ascospores with sheath. p Germinating ascospore. Scale bars b = 1 mm, c, d, f–i = 100 lm, e, l– p = 20 lm, j = 10 lm cells of textura angularis. Hamathecium comprising 1.8– 3.7 lm wide, dense, septate, hyaline, pseudoparaphyses. Asci 75–115 9 22–29 lm ( x = 94 9 25 lm, n = 10), 8spored, bitunicate, fissitunicate, cylindric-clavate, short pedicellate, thick-walled at the apex, with a minute ocular chamber. Ascospores 20–29 9 9–13 lm ( x = 24 9 11 lm, n = 20), overlapping 1–2-seriate, hyaline when young, becoming golden yellow at maturity, muriform, 5–7 transverse septate with 2–3 longitudinal septa in all cells and rarely in end cells, slightly constricted in the middle, conical and narrowly rounded at the ends, surrounded by large mucilaginous sheath. Asexual morph Undetermined. Culture characteristics: Colonies on MEA 60 mm diam. after 4 weeks at 16 °C, circular colony with entire edge, smooth, raised on surface, white-grey. Material examined: ITALY, near Mezzana, province of Trento [TN], dead stem of Thalictrum sp. Tourn. ex L. (Ranunculaceae), 16 July 2014, Erio Camporesi, IT1998B (MFLU 15-1491, holotype); ex-type living culture, MFLUCC 15-0073); ibid. (HKAS 94599 bis, paratypes). Notes: Comoclathris lanata is the type species of Comoclathris which was introduced by Clements (1909). The genus is characterized by ascomata with circular lid-like openings and applanate, reddish-brown to dark reddishbrown, muriform ascospores, with a single longitudinal septum (Shoemaker and Babcock 1992). The phylogenetic classification in this study shows that Comoclathris italica clusters with C. spartii (Fig. 66), although it is differentiated from C. spartii based on molecular phylogeny and morphological support. Our new taxon is characterized by ascomata with ostioles and golden yellow ascospores with conical and narrowly rounded ends, while C. spartii has yellow to pale brown ascospores with broadly fusiform, obtuse ends, constricted at primary septum (Crous et al. 2014). Roussoellaceae J.K. Liu et al. Roussoellaceae was introduced by Liu et al. (2014) and is typified with Roussoella. This family is characterized by immersed, clypeate, gregarious ascostromata, cylindrical, bitunicate asci, and brown, 2-celled ascospores, and is phylogenetically close with Biatriosporaceae and Pleomassariaceae (Liu et al. 2014; Wijayawardene et al. 2014a). Appendispora, Cytoplea, Elongatopedicellata, Neoroussoella, Roussoella and Roussoellopsis are placed in this family (Ariyawansa et al. 2015a), and members mostly occur on bamboo and palms in terrestrial habitats. 119 In this study we introduce a new species of Neoroussoella with support from molecular data (Fig. 68). Neoroussoella lenispora J.F. Zhang, J.K. Liu, K.D. Hyde & Z.Y. Liu, sp. nov. Index Fungorum number: IF552324; Facesoffungi number: FoF02443, Fig. 69 Etymology: Name reflects the ascospores being smoothwalled, from the Latin lenis referring to smooth and spora. Holotype: GZAAS 16-0011. Saprobic on decaying branch. Sexual morph Ascostromata semi-immersed to erumpent, clypeate, globose to subglobose, solitary to gregarious, black, coriaceous, centrally ostiolate, visible as black, dome-shaped spots. Peridium up to 22–45 lm, composed of an outer stratum of dark brown, thick-walled cells fusing with host tissue, and an inner stratum of pale brown, thin-walled cells arranged in a textura prismatica. Hamathecium comprising 1.4– 2.8 lm wide, anastomosing pseudoparaphyses, branched at apex, and embedded in a gelatinous matrix. Asci (74–)89– 113(–127) 9 (5.9–)6.5–7(–9) lm ( x = 101 9 6.8 lm, n = 20), 8-spored, bitunicate, cylindrical, long pedicellate, with a furcate pedicel, apically rounded with a minute ocular chamber. Ascospores (10–)12–14.5(–16) 9 (3.5–) 4.5–5 lm ( x = 13 9 4.5 lm, n = 30), uni- to bi-seriate or overlapping, ellipsoidal to fusiform, 2-celled, constricted at the septum, pale brown to brown, guttulate when young, smooth-walled, without any mucilaginous sheath and appendages. Asexual morph Undetermined. Culture characteristics: Ascospore geminating on WA within 12 h and colonies on PDA reaching 24 mm diam. in 10 days at 25 °C; circular, irregular, aerial hypha dense, raised, white to pale brown in forward and rufous in reverse. Mycelium superficial to immersed in/on media, with branched, smooth-walled hyphae. Material examined: CHINA, Guizhou, Libo County, Maolan National Natural Preserve, on decaying branch of unidentified host, 8 July 2015, J.F. Zhang, MLC-13 (GZAAS 16-0011, holotype), ex-type living culture, GZCC16-0020. Notes: The new taxon is typical of species in the family Roussoellaceae, and the sequence data also shows that it is most phylogenetically close to Neoroussoella bambusae (Fig. 68). However, it differs in the morphology of ascostromata. In N. lenispora ascostroma are globose or subglobose, while in N. bambusae, they are flattened at the base. The asci and ascospores also differ (asci: 101 9 6.8 lm vs. 72.2 9 5.6 lm; ascospores: 13 9 4.5 lm vs. 9 9 3 lm). The ascospores of N. bambusae are ornamented with longitudinal ribs and surrounded by a mucilaginous sheath, whereas those of N. lenispora lack a sheath and are smooth-walled when mature. 123 120 123 Fungal Diversity (2016) 80:1–270 Fungal Diversity (2016) 80:1–270 b Fig. 81 Phylogram generated from maximum likelihood analysis based on combined LSU and SSU sequence data of selected taxa. Maximum likelihood bootstrap support values greater than 60 % are given above the nodes. The type strains of each genera are in bold and the new isolates are in blue bold. The tree is rooted with Arthoniomycetes taxa Torulaceae Corda = Dendryphiaceae Corda, Icon. fung. (Prague) 4: 32 (1840) Corda introduced the family Torulaceae (Sturm 1829). Crous et al. (2015a) revisited this family, provided molecular data for four species of Torula; accepted two genera (Dendryphion and Torula) and placed Torulaceae in the order Pleosporales. Su et al. (2016a) introduced a new genus Neotorula in this family and provided molecular data for three species of Dendryphion including two new species. Presently, there are three genera accepted in the family Torulaceae (Su et al. 2016a). Torulaceae is known only by asexual taxa characterized by erect, micro- or macronematous conidiophores, with or without apical branches, and doliiform to ellipsoid or clavate, brown, smooth to verruculose, mono- to polyblastic, often cupulate, conidiogenous cells and subcylindrical, phragmosporous, acrogenous, brown, dry, smooth to verrucose conidia, characteristically produced in branched chains (Crous et al. 2015a; Su et al. 2016a). A phylogenetic tree for the family is presented in this paper (Fig. 70). Neotorula Ariyawansa et al. Neotorula was established by Su et al. (2016a) to accommodate a Torula-like species collected from freshwater habitats in China, based on both morphology and phylogeny. Neotorula shares similarities with Torula in having clavate to subcylindrical, brown conidia, with rounded ends that are borne in branched chains. The young conidia of N. aquatica are pale green, and are pale yellow in N. submersa. The conidiogenesis in Neotorula is polytretic, whereas Torula has polyblastic cupulate and darkcoloured conidiogenous cells. Neotorula has distinct conidiophores which comprise a few cells, while comprise a single or few cells in Torula. Our new species have polytretic, macronematous conidiophores. In this study, we introduce a new species in Neotorula based on morphological characters. The phylogenetic analysis also supports our taxon as a new species (Fig. 70). Neotorula submersa Z.L. Luo, H.Y. Su & K.D. Hyde, sp. nov. Index Fungorum number: IF552138; Facesoffungi number: FoF02217, Fig. 71 Etymology: With reference to the submerged habitat. Holotype: HKAS 92660. 121 Saprobic on submerged decaying wood. Sexual morph Undetermined. Asexual morph Colonies on the substrate superficial, effuse, gregarious, hairy, dark brown. Mycelium immersed, composed of septate, branched, thin-walled, smooth, pale brown hyphae. Conidiophores mononematous, macronematous, branched or unbranched, septate, erect, straight or flexuous, smooth, pale brown, cylindrical, 2–5-septate, 18–48 lm ( x = 33 lm, SD = 15, n = 10) long, 4–5 lm ( x = 4.5 lm, SD = 0.5, n = 10) wide. Conidiogenous cells mono- to polytretic, integrated or discrete, terminal, pale brown, doliiform or lageniform. Conidia acrogenous, in short chains, dry, clavate to subcylindrical, rounded at the apex, 2–4-septate, dark bands at the septa, verruculose, pale yellow when young, tawny to brown when mature, 16–22 lm ( x = 19 lm, SD = 3, n = 25) long, 5–6 lm ( x = 5.5 lm, SD = 0.5, n = 25) wide. Conidial secession schizolytic. Material examined: CHINA, Yunnan Province, saprobic on decaying wood submerged in Dulong River, May 2015, X.C. Tao, HD 1–10–7 (HKAS 92660, holotype), ex-type culture, KUMCC 15-0280, MFLUCC. Notes: Neotorula submersa was collected from northwestern Yunnan Province. It differs from N. aquatica in having thinner conidiophores (4–5 lm vs. 6–7 lm) and longer conidia (16–22 lm vs. 11–16 lm). The conidia of N. aquatica are pale green when young and brown when mature, in N. submersa they are pale yellow when young and tawny to brown when mature. Phylogenies also reveal a close relationship between these two taxa (Fig. 70). Tubeufiales Boonmee & K.D. Hyde For Tubeufiales, we follow Boonmee et al. (2014) Tubeufiaceae M.E. Barr The most recent treatments of the family and order are Boonmee et al. (2014) and their findings are followed here. In this study we provide and updated backbone tree for Tubeufiaceae (Fig. 72) and introduce Chlamydotubeufia helicospora, Helicoma guttulatum, Neoacanthostigma septoconstrictum, Tubeufia hyalospora and T. roseoelicospora. A reference specimen is also designated for Aquaphila albicans. Aquaphila Goh et al. Aquaphila is a genus known only from freshwater habitats and is typified by A. albicans which is connected with the sexual morph Tubeufia asiana Sivichai & K.M. Tsui (Tsui et al. 2007). Boonmee et al. (2014) synonymized T. asiana under Aquaphila and named Aquaphila asiana based on phylogenetic evidence. Aquaphila albicans Goh, K.D. Hyde & W.H. Ho, Mycol. Res. 102(5): 588 (1998) 123 122 Fig. 82 Alloarthopyrenia italica (holotype). a Herbarium material. b, c Ascostromata on host surface. d Section of ascostroma. e Ostiole. f Section of peridium. g Pseudoparaphyses. h–i Ocular chamber (i ocular chamber staining in Melzer’s reagent). j Immature asci. 123 Fungal Diversity (2016) 80:1–270 k–l Mature asci. m Ascus staining in Melzer’s reagent. n–r Ascospores. Scale bar b = 200 lm, c = 100 lm, d = 20 lm, e–m = 50 lm, h–i = 5 lm, n–r = 10 lm Fungal Diversity (2016) 80:1–270 Index Fungorum number: IF443558; Facesoffungi number: FoF02356, Fig. 73 Reference specimen: MFLU 16-1136 Saprobic on decaying wood in flowing freshwater stream. Colonies on natural substrate effuse, translucent, chalky white or pale yellowish when dry. Mycelium partly immersed in woody substrate and partly superficial, consisting of septate, branched, hyaline, smooth, thin-walled hyphae. Sexual morph Undetermined. Asexual morph hyphomycetous; phragmosporous. Conidiophores arising singly as lateral branches from procumbent hyphae, up to 33 lm long, 4–6 lm wide, simple or branched, thin-walled and smooth, indistinctly septate, flexuous, hyaline. Conidiogenous cells monoblastic or polyblastic, sympodial with cylindrical denticles, hyaline, with numerous integrated tiny pegs. Conidia 70–86 9 8–10 lm, holoblastic, acrogenously borne on denticles, solitary, obclavate, predominantly fusoid to sickle-shaped, sometimes sigmoid, slightly curved and acute at both ends, basal cell obconical, up to 14-euseptate, slightly constricted at the septa, hyaline to pale yellowish, densely guttulate, smooth-walled. Culture characteristics: Conidia germinating on water agar (WA) within 12 h and germ tubes produced at both ends. Colonies on malt extract agar (MEA), reaching 10 mm in 4 weeks at 28 °C, brown to dark brown in MEA media. Mycelium superficial and partially immersed, branched, septate, hyaline to pale brown, smooth. Material examined: THAILAND, Prachuap Khiri Khan, Bang Sapan, Ron Thai, N11°140 47.02800 E99°190 59.68200 , elev. ca. 171 msl., on decaying wood in flowing freshwater stream, 30 July 2015, K.D. Hyde TF04 (MFLU 16-1136, reference specimen designated here), MFLU 16-1138, BBH 41052,; ex-type living culture, MFLUCC 16-0010, TBRC. Sequence data: LSU = KX454166 and KX454168, ITS = KX454165 and KX454167. Notes: Two new collections of Aquaphila albicans (MFLU 16-1136 and MFLU 16-1138) were found on submerged wood in southern Thailand. Their morphology is identical to the type species A. albicans (HKU (M) 2856) collected in Queensland, Australia (Goh et al. 1998). Additionally, phylogenetic analysis placed the two new specimens in the same clade with other A. albicans strains and its sexual morph A. asiana (strain BCC3463) with high support (Fig. 72). We therefore designate MFLU 16-1136 as a reference specimen (sensu Ariyawansa et al. 2014a, b, c) for Aquaphila albicans. We do not designate an epitype as this was not collected from Australia. Chlamydotubeufia Boonmee & K.D. Hyde Chlamydotubeufia is a genus recognized by black pigmented with multi-septate dictyochlamydosporous conidia and a typical sexual morph (Boonmee et al. 2011). 123 Chlamydotubeufia a small genus with only four species presently recorded and typified by Ch. huaikangplaensis Boonmee & K.D. Hyde (Index Fungorum 2016). Chlamydotubeufia helicospora Boonmee, Y.Z. Lu & K.D. Hyde, sp. nov. Index Fungorum number: IF552217; Facesoffungi number: FoF02357, Fig. 74 Holotype: MFLU 16-1338. Etymology: ‘helicospora’ referring to asexual helicospores. Saprobic on woody substrate. Mycelium partly immersed, partly superficial, white, septate, sparsely branched hyphae, with masses of crowded conidia. Sexual morph Undetermined. Asexual morph Conidiophores 15–25 lm long 9 4–6 lm wide ( x = 18 9 5 lm, n = 10), pale brown, macronematous, erect, short, smooth-walled. Conidiogenous cells 11–33 lm long 9 4–5 lm wide ( x = 18 9 4.5 lm, n = 10), monoblastic, integrated, each with single conidium. Conidia 63–119 lm diam. and conidial filament 5–7 lm wide ( x = 80 9 6 lm, n = 20), 405–546 lm long, loosely coiled 1–2.5 times, rounded at apical end, up to 68-septate, slightly constricted at septa, hyaline, smooth-walled. Culture characteristics: Conidia germinating on water agar (WA) within 8 h and germ tubes produced from conidia. Colonies on malt extract agar (MEA) reaching 4 mm in 1 week at 28 °C, brown to dark brown. Mycelium superficial and partially immersed, branched, septate, hyaline to pale brown, smooth. Material examined: THAILAND, Uttaradit, Laplae, Mae Phun, Ban Ton Klua, on decaying wood in flowing freshwater stream, 24 October 2015, Saranyaphat Boonmee, UTD15–1 (MFLU 16-1338, holotype, BBH 41053, isotype); ex-type living culture, MFLUCC 16-0213, TBRC. Notes: According to phylogenetic analysis, Chlamydotubeufia helicospora clusters in the Chlamydotubeufia clade with high support. The molecular analyses (Fig. 72) confirms C. helicospora as a new taxon that is basal to C. khunkornensis, while C. chlamydospora and C. huaikangplaensis constitutes a different sublineage with very high statistical support. Hence, we assign our collection as a new species based on morphology and phylogeny (Fig. 72). Morphologically, C. helicospora is characterized by slender hyphae, moderately long conidiophores and helicosporous conidia. The conidia are different from other species in Chlamydotubeufia (Boonmee et al. 2011). Helicoma Corda The genus Helicoma was established by Corda with the type species H. muelleri Corda. The genus is distinguished by its relatively short, erect, thick, dark brown, smooth conidiophores, holoblastic conidiogenous cells and 123 124 Fungal Diversity (2016) 80:1–270 Neotrematosphaeria biappendiculata KTC 1124 Coelodictyosporium muriforme MFLUCC 13 0351 Lophiopoacea paramacrostoma MFLUCC 11 0463 ravennica MFLUCC 14 0005 Lophiohelichrysum helichrysi MFLUCC 15 0701 Guttulispora crataegi MFLUCC 13 0442 Platystomum crataegi MFLUCC 14 0925e Lophiostoma compressum IFRD 2014 Lophiostoma macrostomum KT508 Biappendiculispora japonica MAFF 239452 79 Capulatispora sagittiformis JCM 15100 93 Platystomum scabridisporum BCC 22835 Trematosphaeria terricola SC 12 Paucispora quadrispora MAFF 239455 85 96 Dimorphiopsis brachystegiae CPC 22679 Vaginatispora aquatic MFLUCC 11 0083 98 Angustimassarina populi MFLUCC 13 0034 75 Massarina corticola CBS 154.93 100 Angustimassarina quercicola MFLUCC 14 0506 Angustimassarina acerina MFLUCC 14 0505 Exosporium stylobatum CBS 160.30 Camarographium carpini CBS 128781 99 Preussia lignicola CBS 264.69 94 Sporormia lignicola CBS 363.69 Preussia minima AFTOL ID1256 90 Preussia funiculate 2577 100 Preussia funiculate CBS 659.74 Preussia terricola AFTOL ID 282 74 Westerdykella dispersa CBS 50875 Westerdykella ornate CBS 379 55 98 97 Eremodothis angulate CBS 610.74 Westerdykella cylindrical CBS 454.72 Sporormia fimetaria Gr.81.194 100 Decaisnella formosa BCC 25617 97 Decaisnella formosa BCC 25616 100 Lignosphaeria thailandica MFLUCC 11 0376 Lignosphaeria fusispora MFLUCC 11-0377 100 Berkleasmium nigroapicale BCC 8220 Bahusandhika indica GUFCC 18001 100 Misturatosphaeria kenyensis GKM L100Na Pseudoaurantiascoma kenyense GKM 1195 95 Misturatosphaeria minima ANM 933 Misturatosphaeria minima GKM 169N Curreya grandicipis CBS 114272 Misturatosphaeria claviformis GKM 1210 Macrodiplodiopsis desmazieri MFLUCC 12 0088 100 Floricola striata JK 5678I Floricola striata JK 5603K Misturatosphaeria tennesseensis ANM 911 Pseudomisturatosphaeria cruciformis SMH 5151 Floricola viticola IT 2178 Misturatosphaeria aurantonotata GKM 1238 Misturatosphaeria radicans ATCC 42522 Ramusculicola thailandica MFLUCC 13 0284 Neomassarina thailandica MFLUCC 10 0552 1 Sigarispora 6 98 100 GU479796_Pontoporeia_biturbinata 93 Halotthia posidoniae BBH 22481 Mauritiana rhizophorae BCC 28866 Sulcosporium thailandica MFLUCC 12-0004 Neolophiostoma pigmentatum MFLUCC 10 0129 Brunneoclavispora bambusae MFLUCC 11- 0177 Roussoellopsis tosaensis KT 1659 Roussoella pustulans KT 1709 Roussoella hysterioides HH 26988 95 Arthopyrenia salicis CBS 368.94 Arthopyrenia salicis CBMAI1330 74 Roussoella angustior MFLUCC 15-0186 Roussoella magnatum MFLUCC 15-0185 97 Neoroussoella bambusae MFLUCC 11-0124 Sporidesmium_australiense 100 Torula herbarum CBS 111855 98 Torula herbarum CBS 379.58 98 Torula herbarum CBS 220.69 100 Dendryphion europaeum CPC 22943 Dendryphion europaeum CPC 23231 Parameliola acaciae MFLU 15–0378 100 Parameliola dimocarpi MFLU15–0045 100 Pseudocoleodictyospora tectonae MFLUCC 12 0387 Pseudocoleodictyospora_tectonae MFLUCC 12 0385 72 Pseudocoleodictyospora thailandica MFLUCC 12 0565 100 Pseudocoleodictyospora sukhothaiensis MFLUCC 12 0554 Subglobosporium tectonae MFLUCC 12 0393 100 Subglobosporium tectonae MFLUCC 12 0390 84 Occultibambusa bambusae MFLUCC 13-0855 87 Occultibambusa bambusae MFLUCC 11-0394 98 Occultibambusa chiangraiensis MFLUCC 16–0380 Occultibambusa aquatica MFLUCC11–0006 Occultibambusa pustula MFLUCC 11-0502 Versicolorisporium triseptatum JCM 14775 Occultibambusa fusispora MFLUCC 11-0127 100 Seriascoma didymospora_MFLUCC 11 0179 Seriascoma didymospora MFLUCC 11 0194 Neooccultibambusa chiangraiensis MFLUCC 12 0559 94_Biatriospora mackinnonii CBS 674.75 100 Nigrograna mackinnonii E5202H Biatriospora marina CY_1228 100 Paradictyoarthriinium diffractum MFLUCC 12-0557 Paradictyoarthriinium tectonicola MFLUCC 13-465 Stagonospora pseudocaricis HKUCC 10833 Neottiosporina paspali CBS 331.37 Corynespora leucadendri CBS 135133 Corynespora olivacea CBS 114450 Massarina eburnea CBS 473.64 Pseudodidymosphaeria phlei MFLUCC14-1061 Deniquelata barringtoniae MFLUCC 110422 94 49 Kalmusia brevispora KT 1466 85 Montagnula aloes CPC 19671 100 Pseudocamarosporium pini MFLUCC 14-1091 Didymosphaeria rubi-ulmifolii MFLUCC 14-0023 96 Bambusicola splendida MFLUCC 11-0439 Bambusicola massarinia MFLUCC 11-0389 100 Latorua caligans CBS 576.65 Latorua grootfonteinensis CBS 369.72 Katumotoa bambusicola KT 1517a 86 Lentithecium fluviatile CBS 122367 Pseudomonodictys tectonae MFLUCC 12 0552 Trematosphaeria pertusa CBS 122371 79 Falciformispora lignatilis BCC 21118 Macrodiplodiopsis desmazieri CPC 24971 99 Macrodiplodiopsis desmazieri CBS 125026 Macrodiplodiopsis desmazieri CPC 24648 100 Moroshperia ramunculicola BCC 18404 85 Moroshperia ramunculicola BCC 18405 Helicascus_nypae BCC 36752 Longiostiolum tectonae MFLUCC 12 0562 Thyridaria rubronotata CBS 385.39 Massariosphaeria phaeospora CBS 611.86 100 Julella avicenniaea BCC 18422 Julella avicenniae BCC 20173 100 Cucurbitaria berberidis CBS 363.93 82 Cucurbitaria berberidis CBS 394.84 Pyrenochaeta nobilis CBS 407.76 89 Camarosporium quaternatum DQ377884 Camarosporium aloes CPC 21572 Phaeosphaeria oryzae CBS 110110 94 79 Ophiosphaerella herpotricha AFTOL-ID 1569 Paraphoma radicina CBS 111.79 100 Neophaeosphaeria filamentosa CBS 102203 100 Neophaeosphaeria filamentosa CBS 102202 Neophaeosphaeria agaves CPC 21264 83 Dothidotthia symphoricarpi CPC 12929 Dothidotthia aspera CPC 12933 100 Coniothyrium palmarum CBS 758.73 Coniothyrium palmarum CBS 400.71 100 Alternariaster helianthi CBS 134020 Alternariaster helianthi CBS 327.69 Leptosphaeria maculans AFTOL-ID 277 75 Pleospora herbarum CBS 191.86 100 Alternaria_alternata AFTOL-ID 1610 Pyrenophora phaeocomes AFTOL-ID 283 72 Phoma exigua CBS 431.74 97 Leptosphaerulina australis CBS 317.83 Didymella exigua CBS 183.55 100 Ascocylindrica marina MD6011 Ascocylindrica mari MD6012 97 Corynespora smithii CABI 5649b Corynespora cassiicola CBS 100822 72 Beverwykella pulmonaria CBS 283.53 100 Melanomma pulvis-pyrius CBS 371.75 Byssosphaeria jamaicana SMH 1403 77 Lindgomyces ingoldianus ATCC 200398 73 Lindgomyces rotundatus KH 114 91 Massariosphaeria typhicola MAFF 239218 Lolia aquatic MF644 97 Anguillospora longissimi CCMF 10304 Anguillospora_longissima CS869-1D Amniculicola lignicola Ying01 Anguillospora longissima F00980 Amniculicola parva CBS 123092 Spirosphaera cupreorufescens A20 100 Amniculicola immersa CBS 123083 100 Repetophragma ontariense HKUCC 10830 96 Massariosphaeria grandispora CBS 613 86 70 Pseudomassariosphaeria bromicola MFLUCC 15–0031 Murispora rubicunda IFRD 2017 Hysterium angustatum CBS 236.34 100 0.05 123 Fungal Diversity (2016) 80:1–270 b Fig. 83 RAxML maximum likelihood phylogenetic tree based on a LSU and SSU sequenced data from species of order Pleosporales. Maximum likelihood bootstrap support values greater than 50 % are shown in near the nodes. Some branches were shortened to fit the page—these are indicated by two diagonal lines with the number of times a branch was shortened indicated next to the lines. The new isolates are in red. The tree is rooted with Hysterium angustatum helicoid, hyaline, thick-walled, brown to dark brown conidia forming from terminal, denticulate conidiophores. Helicoma guttulatum Y.Z. Lu, Boonmee & K.D. Hyde, sp. nov. Index Fungorum number: IF552218; Facesoffungi number: FoF02358, Fig. 75 Holotype: MFLU 16-1339 Etymology: ‘guttulatum’ referring to conidia containing numerous guttules. Saprobic on woody substrates. Mycelium composed of partly immersed and partly superficial, pale brown, septate hyphae. Sexual morph Undetermined. Asexual morph Conidiophores 74–182(197) lm long, 4–6 lm wide ( x = 120 9 5 lm, n = 20), macronematous, crowded, erect, subhyaline to yellowish, brown towards the base, septate, unbranched, smooth-walled. Conidiogenous cells monoblastic to polyblastic, subhyaline to pale brown, smooth-walled. Conidia 18–23 lm diam., and conidial filament 6–8 lm wide ( x = 20 9 7 lm, n = 20), tightly coiled 1–1 times, hyaline to pale brown, tapering toward flat end, 8–9-septate, rounded at the apex, conico-truncate at the base, smooth-walled. Culture characteristics: Conidia germinating on water agar (WA) within 12 h. Colonies on malt extract agar (MEA) reaching 5 mm in 1 week at 28 °C, pale brown to brown. Mycelium superficial and partially immersed, branched, septate, hyaline to pale brown, smooth. Material examined: THAILAND, Chiang Rai, Muang, Ban Nang Lae Nai, on decaying wood in flowing freshwater stream, 28 November 2015, Yong-Zhong Lu and Saranyaphat Boonmee, TUB02–1 (MFLU 16-1339, holotype, BBH 41054, isotype); ex-type living culture, MFLUCC 16-0022, TBRC. Notes: Helicoma guttulatum was collected from northern Thailand. Phylogenetic results recognize H. guttulatum as belonging to the genus Helicoma as well as its recognition as a new species. Helicoma guttulatum segregates from H. dennisii and H. inthanonense with full support in the phylogenetic analysis (Fig. 72). Helicoma guttulatum forms an asexual morph on the natural substrate, which is morphologically similar to H. muelleri (Boonmee et al. 2014). However, its conidiophores (74–182 lm) are longer and narrower (4–6 lm) than H. muelleri (80–154 lm) and (7–10 lm). The conidial diameter (18–23 lm) is also 125 greater than H. moelleri (16–19 lm). Therefore, H. guttulatum is introduced here as a novel species. Neoacanthostigma Boonmee et al. The genus Neoacanthostigma was introduced to accommodate three sexual taxa, characterized by dark pigmented ascomata, surrounded by reddish-brown to dark brown setae, bitunicate asci, fasciculate cylindrical and hyaline ascospores, and having a helicosporous asexual state (Boonmee et al. 2014). Here we collected Acanthostigma septoconstrictum Promp. & A.N. Mill. and transfer it to Neoacanthostigma. Neoacanthostigma septoconstrictum (Promp. & A.N. Mill.) S. Boonmee & K.D. Hyde, Fungal Diversity 68(1): 279 (2014) : Acanthostigma septoconstrictum Promp. & A.N. Mill., Mycologia 102(3): 579 (2010) Index Fungorum number: IF550683; Facesoffungi number: FoF02360, Fig. 76 Reference specimen: MFLU 16-1134 Saprobic on decaying wood in flowing freshwater stream. Mycelium partly superficial, partly immersed, composed of brown, septate, sparsely branched hyphae, with masses of crowded conidia. Sexual morph Undetermined. Asexual morph hyphomycetous; helicosporous. Conidiophores brown, macronematous, erect, short, smooth-walled. Conidiogenous cells monoblastic, integrated, each with single conidium. Conidia 115–155 lm diam., filament 9–11 lm wide ( x = 139 9 10 lm, n = 20), 775–920 lm long, loosely coiled 1–2 times, rounded at apical end, up to 71-septate, not constricted at septa, pale brown to brown, smooth-walled. Culture characteristics: Conidia germinating on water agar (WA) within 12 h and germ tubes produced from conidia. Colonies growing on malt extract agar (MEA), reaching 25 mm in 4 weeks at 28 °C, slightly convex, with an undulate edge, pale yellow to pale brown, brown in MEA media. Mycelium superficial and partially immersed, branched, septate, hyaline to pale brown, smooth. Material examined: THAILAND, Prachuap Khiri Khan, Bang Sapan, Ron Thai, on decaying wood in flowing freshwater stream, 30 July 2015, K.D. Hyde, KH02 (MFLU 16-1134, BBH 41051); living culture, MFLUCC 15–1248, TBRC. Notes: Neoacanthostigma was introduced by Boonmee et al. (2014) with N. fusiforme as the type species. An asexual morph has not been previously reported in this genus. In this study, N. septoconstrictum was found in its asexual form (Fig. 72) on decaying wood in a flowing freshwater stream and is characterized by pale brown to brown, large filament, multi-septate conidia. Phylogenetic analysis placed our collection in a cluster with the sexual 123 126 Fungal Diversity (2016) 80:1–270 Fig. 84 Neomassarina thailandica (holotype). a Appearance of ascostromata on the host surface. b Section through the ascostroma. c Section through peridium. d Pseudoparaphyses stained in Melzer’s reagent. e–h Asci. i–k Ascospores. l Ascospores becoming pale brown at maturity. m, n Culture characteristics (m = from above, n = from below). Scale bars b = 100 lm, c = 20 lm, d–h, l = 10 lm, i–k = 5 lm morph N. septoconstrictum (strain ANM 536.1) with high support (Fig. 72). Therefore, we describe this fungus under the sexual name N. septoconstrictum. Tubeufia hyalospora Y.Z. Lu, Boonmee & K.D. Hyde, sp. nov. Index Fungorum number: IF552220; Facesoffungi number: FoF02361, Fig. 77 Holotype: MFLU 16-1135 Etymology: ‘hyalospora’ referring to hyaline helicospores of asexual morph. Saprobic on decaying woody in flowing freshwater stream. Mycelium partly immersed, partly superficial, composed of pale brown, septate, sparsely branched Tubeufia Penz. & Sacc. The genus Tubeufia is the type genus of the family Tubeufiaceae. It is characterized by white, cream-pink to brownish, vertically oblong to ovoid ascomata and cylindrical, fusiform to vermiform, multi-septate ascospores (Barr 1980). 123 Fungal Diversity (2016) 80:1–270 127 Fig. 85 Phylogenetic tree of Aspergillus koreanus and related species based on maximum parsimony analysis of a combined dataset from RNA polymerase II (RPB2), ITS and 28S rDNA sequence data. Sequences data from Talaromyces bacillisporus was used as the outgroup taxon. Numbers at the nodes indicate the bootstrap values ([50 %) from 1000 replicates. The bar indicates the number of substitutions per position. New taxa are in blue hyphae, with masses of crowded conidia. Sexual morph Undetermined. Asexual morph hyphomycetous; helicosporous. Conidiophores pale brown, macronematous, erect, short, smooth-walled. Conidiogenous cells monoblastic, integrated, each with single conidium. Conidia 16–33 lm diam., conidial filament 3–5 lm wide ( x = 22 9 4 lm, n = 20), 110–225 lm long, coiled 2– 3 times, rounded at apical end, multi-septate, slightly constricted at septa, hyaline, smooth-walled. Culture characteristics: Conidia germinating on water agar (WA) within 12 h and germ tubes produced from conidia. Colonies growing on malt extract agar (MEA), reaching 26 mm in 3 weeks at 28 °C, with an undulate edge, pale brown to brown, dark brown, white at margin. Mycelium superficial and partially immersed, branched, septate, hyaline to pale brown, smooth. Material examined: THAILAND, Prachuap Khiri Khan, Bang Sapan, Ron Thai, on decaying wood in flowing 123 128 Fungal Diversity (2016) 80:1–270 Fig. 86 Phylogenetic tree of Aspergillus koreanus and related species within the sect. Cremei based on maximum parsimony analysis of ITS sequence data. Sequence data for Aspergillus tamarii was used as the outgroup taxon. Numbers at the nodes indicate the bootstrap values ([50 %) from 1000 replicates. The bar indicates the number of substitutions per position. New taxa are in blue freshwater stream, 30 July 2015, K.D. Hyde, TF03 (MFLU 16-1135, holotype); ex-type living culture, MFLUCC 15-1250, TBRC. Notes: Tubeufia hyalospora grouped within the Tubeufia clade, but did not cluster with any known species (Fig. 72). Tubeufia hyalospora forms an asexual morph on the natural substrate, which is morphologically similar to T. tectonae (Doilom et al. 2016). Its conidia (diam. 16–33 lm) are narrower than those of T. tectonae (32–55 lm) and are tightly coiled, but in T. tectonae most are loosely coiled or uncoiled. Holotype: MFLU 16-1133. Saprobic on decaying woody in flowing freshwater stream. Colonies on natural substrate, superficial with partly immersed mycelium and bright, septate, sparsely branched hyphae, with masses of crowded conidia. Sexual morph Undetermined. Asexual morph Hyphomycetes, helicosporous. Conidiophores macronematous, erect, short, pale brown, smooth-walled. Conidiogenous cells monoblastic, integrated, each with single conidium. Conidia 36–48 lm diam., filaments 5–7 lm wide ( x = 41 9 6 lm, n = 20), 210–283 lm long, coiled 2– 3 times, tight to loose, rounded at apical end, 29–35septate, slightly constricted at septa, light pink on substrate, hyaline when seen under light microscope, smooth-walled. Culture characteristics: Conidia germinating on water agar (WA) within 12 h and germ tubes produced from conidia. Colonies growing on malt extract agar (MEA) reaching 10 mm in 2 weeks at 28 °C, with an undulate Tubeufia roseohelicospora Y.Z. Lu, Boonmee & K.D. Hyde, sp. nov. Index Fungorum number: IF02362; Facesoffungi number: FoF02362, Fig. 78 Etymology: ‘roseohelicospora’ referring to light pink helicospores of asexual morph. 123 Fungal Diversity (2016) 80:1–270 129 Fig. 87 Phylogenetic tree of Aspergillus koreanus and related species within the sect. Cremei based on maximum parsimony analysis of combined dataset for beta tubulin (BenA), calmodulin (CaM), ITS rDNA, 28S rDNA and RPB2 sequence data. Sequence data for Aspergillus tamarii is used as the outgroup taxon. Numbers at the nodes indicate the bootstrap values ([50 %) from 1000 replicates. The bar indicates the number of substitutions per position. New taxa are in blue edge, pale brown to brown, dark brown. Mycelium superficial and partially immersed, branched, septate, hyaline to pale brown, smooth-walled. Material examined: THAILAND, Prachuap Khiri Khan, Bang Sapan, Ron Thai, on decaying wood in flowing freshwater stream, 30 July 2015, K.D. Hyde KH01 (MFLU 16-1133, holotype; BBH 41050, isotype); ex-type living culture, MFLUCC 15-1247, TBRC. Notes: Tubeufia roseoelicospora clusters in Tubeufia sister to T. tectonae based on the phylogenetic analysis of combined LSU and ITS sequence data (Fig. 72). The conidiophores of T. roseoelicospora are tightly coiled while those of T. tectonae are mostly loosely coiled or uncoiled; its conidia filament is 5–7 lm wide, while that of T. tectonae is 2–5 lm wide. On the natural substrate colonies of T. roseohelicospora are light pink, while T. tectonae colonies are hyaline to white. Therefore, T. hyalospora is introduced here as a novel species based on its morphological and phylogenetic differences from other Tubeufia species. Dothideomycetes family, incertae sedis Pleurotremataceae Walt. Watson Currently Pleurotremataceae comprises the genus Pleurotrema and Dyfrolomyces species may need transferring to this genus (Maharachchikumbura et al. 2016). Whether the freshwater species of Saccardoella belong to this family will require further studies at the molecular level. Pleurotrema Müll. Arg. Watson (1929) introduced the family Pleurotremataceae based on Pleurotrema polysemum (Nyl.) Mull. Arg. Eriksson and Hawksworth (1993) suspected Pleurotremataceae to be synonym of Pyrenulaceae. Pleurotrema was synonymized under Lithothelium as a member of Pyrenulaceae by Aptroot (1991). Later, Harris re- 123 130 Fig. 88 Aspergillus koreanus (holotype). a, d Colonies on malt extract agar (MEA). b, e Colonies on yeast extract sucrose agar (YES). e, f Colonies on Czapek yeast autolysate agar (CYA) (a– c from above, d–f from below). g–i Developing bi-seriate conidial heads on stalks. j Phialides finely differentiated on metulae covering the entire surface of vesicle. k Conidiophore and phialides (green 123 Fungal Diversity (2016) 80:1–270 arrow). l Detail of a metula bearing 3 phialides (red arrow). m Initial young conidia (yellow arrow) developed at the tips of phialides and scars remaining after conidial detachment (white arrow). n Mature conidia with slightly roughened scale-like surface. Scale bars g–j, k, l = 20 lm, m, n = 5 lm Fungal Diversity (2016) 80:1–270 examined the isotype of P. polysemum, and showed its similarity to Melomastia and Saccardoella (Barr 1994). Based on its cylindrical asci, with a non amyloid apical ring and hyaline, distoseptate ascospores Barr (1994) transferred Pleurotremataceae to Xylariales. Five genera, Phomatospora, Melomastia, Pleurotrema, Saccardoella, Daruvedia, were included in Pleurotremaceae on account of their non-fissitunicate asci (Barr 1994). Hawksworth et al. (1995) disagreed with this justification and retained Pleurotrema in the order Pyrenulales. Saccardoella and Melomastia were placed in Ascomycota genera incertae sedis (Kirk et al. 2001; Lumbsch and Huhndorf 2010), but there is no phylogenetic evidence to support this. Pleurotremataceae was accepted as monotypic via Pleurotrema and placed under Chaetosphaeriales without giving a reason by Maharachchikumbura et al. (2015). However, Maharachchikumbura et al. (2016) excluded this family from class Sordariomycetes and this is an earlier name for Dyfrolomycetaceae (Dothideomycetes). Pleurotrema thailandica Dayarathne, Jones E.B.G. & K.D. Hyde, sp. nov. Index Fungorum number: IF552244; Facesoffungi number: FoF02445, Fig. 80 Etymology: Name reflects the country Thailand, from where the holotype was collected. Holotype: MFLU 16-1173. Saprobic on pneumatophores of Avicennia marina in mangrove vegetation. Sexual morph Ascomata 430– 350 lm diam. 9 242–280 lm high ( x = 428 9 264 lm, n = 10), solitary, semi-immersed, clypeate, globose to subglobose, dark brown to black, ostiolate, ostiolar canal filled with dark cells, apapillate. Peridium 26–35 lm wide, comprising an outer layer of dark brown cells of textura angularis, becoming lighter inwardly. Hamathecium comprising numerous, 2.1–3.6 lm wide, septate pseudoparaphyses embedded in a gelatinous matrix. Asci 146– 158 9 7–9 lm ( x = 152 9 8.2 lm, n = 10), 8-spored, bitunicate, cylindrical, short-pedicellate, with a thickened apex. Ascospores 24–32 9 6–8 lm ( x = 28 9 6.5 lm, n = 20), slightly overlapping uni-seriate, hyaline, ellipsoidal, mostly 3–5-septate, slightly constricted at septa, with several small guttules, surrounded by a gelatinous sheath, 1.2–2.4 lm thick. Asexual morph Undetermined. Culture characteristics: Colonies on PDA, reaching 3 cm in 14 days at 25–28 °C, white at first, becoming yellowish when mature, undulate and reverse yellowishwhite. Material examined: THAILAND, Phetchaburi Province, Hat Chao Samran, 47°725060 E, 40°250380 N, 0 m asl., on pneumatophores of Avicennia marina, 28 July 2015, Monika Dayarathne, CHAM 006 (MFLU 16-1173, holotype); ex-type living culture, MFLUCC 15-0945, ICMP. 131 Notes: Pleurotrema thailandica is similar to other species of Pleurotrema, but can be differentiated based on ascospore characteristics, in particular ascospore septation. Ascospores of P. thailandica are 3–5-septate, while those of P. tiomanensis, P. mangrovei, P. marinospora and P. rhizophorae are 20–24, 7–9, 3 and 4–6 septate, respectively. According to our maximum likelihood analysis based on combined LSU and SSU sequence data, D. thailandica forms a distinct lineage, sister to P. rhizophorae with 99 % bootstrap support (Fig. 79). Trypetheliaceae Eschweiler Trypetheliaceae is mainly a lichen-forming family distributed mostly in tropical to subtropical areas (Harris 1984; Aptroot et al. 2008; Nelsen et al. 2014). The family was introduced by Eschweiler (1824) and is typified by T. eluteriae Sprengel. It is characterized by a corticolous thallus, perithecioid ascomata often organized in pseudostromata, richly branched and anastomosing, net-like pseudoparaphyses, bitunicate asci, ascospores often with angular wall thickenings and diamond-shaped lumina, and a trentepohlioid photobiont in lichenized taxa (Harris 1984; Aptroot 1998, 2008; Hyde et al. 2013; Nelsen et al. 2009, 2014). Based on phylogenetic analyses, the family forms a monophyletic clade in Trypetheliales, sister to Polycoccaceae (Ertz et al. 2015). In this paper we introduce a new genus, Alloarthopyrenia, in Trypetheliaceae to accommodate a unique, new, non-lichenized lineage resembling Arthopyrenia (Fig. 81). Alloarthopyrenia Phukhamsakda, Lücking & K.D. Hyde, gen. nov. Index Fungorum number: IF552236, Facesoffungi number: FoF02379 Etymology: The generic name Alloarthopyrenia refers to its morphological resemblance with Arthopyrenia. Saprobic on living tree branches. Thallus absent but area around the ascomata in part whitish. Sexual morph Ascostromata covered with a blackened pseudoclypeus, semiimmersed, with only ostioles visible, coriaceous, solitary, scattered or gregarious, depressed globose to obpyriform, wall rough, black to dark brown, ostiolate. Ostiole centrally located, filled with periphyses. Peridium dark brown to light brown, cells of textura angularis and textura epidermoidea, easy to break, at base indistinguishable from host tissue. Hamathecium composed of branched, anastomosing, trabeculate pseudoparaphyses, embedded in a gelatinous matrix. Asci 8-spored, bitunicate, obviod to suboblong, short pedicellate, thick-walled, faintly bluish in IKI ? , apically rounded, with ocular chamber, lightly IKI ? bluish. Ascospores partially overlapping or bi-seriate, hyaline, oviod or ellipsoid, septate, constricted at the 123 132 Fungal Diversity (2016) 80:1–270 Unknown rock isolate1 TRN508 Unknown rock isolate3 TRN515 Unknown rock isolate2 TRN210 Cladophialophora australiensis CBS 112793 Cladophialophora minourae CBS 556.83 95/1.00 99/1.00 Cladophialophora carrionii CBS 160.54 Phialophora verrucosa AFTOL ID670 90/1.00 90/1.00 Capronia semiimmersa AFTOL ID658 Cladophialphora devriii CBS 147.84 Fonsecaea_monophora CBS 102243 69/-Cladophialophora emmonsii CBS 640.96 Herpotrichiellaceae Exophiala xenobiotica CBS 115831 Atrokylindriopsis setulosa HMAS245592 86/-Exophiala salmonis AFTOL ID671 Minimelanolocus melanicus MFLUCC15-0415 73/0.99 85/1.00 Minimelanolocus asiaticus MFLUCC15-0237 78/1.00 Minimelanolocus submersus KUMCC15-0206 86/1.00 Minimelanolocus aquaticus MFLUCC15-0414 Minimelanolocus obscurus MFLUCC15-0416 Capronia fungicola CBS 614.96 Capronia mansonii CBS101.67 100/1.00 Capronia munkii AFTOL ID656 94/1.00 Ceramothyrium thailandicum MFLUCC10-0008 Ceramothyrium menglunense MFLUCC14-1120 78/1.00 Chaetothyrium brischoficola MFLUCC 10-0012 100/1.00 Ceramothyrium longivolcaniforme MFLU13-0632b 99/1.00 Ceramothyrium ficus MFLUCC15-0229 75/1.00 Ceramothyrium ficus MFLUCC15-0228 Chaetothyriaceae Ceramothyrium podocarpi CPC19826 68/-Ceramothyrium carniolicum AFTOL ID1063 100/1.00 Ceramothyrium carniolicum CBS 175.95 100/1.00 Phaeosaccardinula multiseptata IFRDCC2639 100/1.00 Phaeosaccardinula ficus MFLUCC10-0009 Phaeosaccardinula dendrocalami IFRDCC2663 Aphanophora eugeniae CBS 124105 62/-Exophiala eucalyptorum CBS121638 62/-Camptophora hylomeconis CBS 113311 Vonarxia vagans CBS 123533 100/1.00 Vonarxia vagans CPC 15152 Brycekendrickomyces acacia CBS 124104 Phaeococcomyces catenatus CBS 650.76 100/1.00 Phaeococcomyces catenatus CPC 13707 Neophaeococcomyces aloes CPC 21873 100/1.00 100/1.00 Bradymyces alpinus CCFEE 5493 Bradymyces oncorhynchi CCF 4369 --/-0.99 Strelitziana malaysiana CPC 24874 100/1.00 92/1.00 Strelitziana syzygii CPC 26591 96/1.00 100/1.00 Strelitziana australiensis CBS 124778 Trichomeriaceae Strelitziana eucalypti CBS 128214 Ceramothyrium melastoma CPC 19837 65/1.00 Trichomerium deniqulatum MFLUCC10-0884 100/1.00 Trichomerium foliicola MFLUCC10-0078 71/-100/1.00 Trichomerium gleosporum MFLUCC10-0087 63/0.95 Knufia epidermidis CBS 120353 100/1.00 Knufia cryptophialidica DAOM 216555 100/1.00 Phaeococcomyces chersonesos Ch49 Knufia perforans CBS 885.95 100/1.00 Metulocladosporiella musae CBS 113863 Metulocladosporiella musae CBS 161.74 100/1.00 Metulocladosporiella musicola CBS 113873 100/1.00 Metulocladosporiella musicola CBS 113865 Cyphellophora reptans CBS 113.85 Cyphellophora sessilis CBS 243.85 73/0.98 Cyphellophora oxyspora CBS 698.73 99/1.00 69/0.98 Cyphellophora europaea CBS 101466 Cyphellophora ambigua CBS 235.93 99/1.00 77/0.99 Cyphellophora eucalypti CBS 124764 Cyphellophora guyanensis MUCL 43737 Cyphellophoraceae Cyphellophora pluriseptata CBS 286.85 --/0.99 Cyphellophora olivacea CBS 122.74 81/0.98 Cyphellophora suttonii CBS 449.91 95/1.00 Cyphellophora laciniata CBS 190.61 Cyphellophora vermispora CBS 228.86 97/1.00 Cyphellophora fusarioides MUCL44033 83/-Cyphellophora pauciseptata CBS 284.85 Epibryon sp.2 M175 Epibryon hepaticola M224 92/1.00 100/1.00 Epibryon hepaticola M10 68/0.94 Epibryon sp.1 M274 100/1.00 95/1.00 Epibryaceae Epibryon turfosorum M292 Epibryon interlamellare M1 100/1.00 Epibryon interlamellare M32 83/1.00 79/1.00 Epibryon interlamellare M223 Epibryon diaphanum M122 Epibryon plagiochilae M187 99/1.00 98/0.99 Catapyrenium daedaleum AFTOL ID2273 0.1 123 Fungal Diversity (2016) 80:1–270 b Fig. 89 Phylogenetic tree generated from maximum parsimony analysis (RAxML) of a combined dataset of LSU and ITS sequence data. Bootstrap support values for maximum likelihood (ML, left), equal to or greater than 60 %, are indicated above or below branches. Bayesian posterior probabilities (PP, right) equal to or greater than 0.95 are indicated on the branches. New isolates are in blue, and other ex-type strains are in bold. The tree is rooted with Catapyrenium daedaleum Fig. 90 Ceramothyrium menglunense (holotype). a, Herbarium material. b Superficial ascoma on substrate. c, d Squash of ascomata wall. e Section through ascoma. f Setae. g Minutely papillate ostiole. 133 septa, wall rough, indentations present when mature, surrounded by a mucilaginous sheath. Asexual morph Undetermined. Type species: Alloarthopyrenia italica Phukhamsakda, Camporesi, Ariyawansa & K.D. Hyde. Notes: Based on phylogenetic analysis, Alloarthopyrenia is introduced as a monospecific genus to accommodate h Pseudoparaphyses. i–j Asci. k–n Ascospores. Scale bars c, e = 100 lm, i, j = 50 lm, d, f, g, h, k–n = 10 lm 123 134 123 Fungal Diversity (2016) 80:1–270 Fungal Diversity (2016) 80:1–270 135 b Fig. 91 Minimelanolocus submersus (holotype). a, b Appearance of the fungus on wood. c–g Conidiophores and conidia. h–m Conidia. n Germinating conidium. o Surface view of culture on PDA. p Reverse view of culture on PDA. Scale bars c = 20 lm, d– e = 25 lm, f = 40 lm, g = 25 lm, h–i = 10 lm, j–m = 20 lm, n = 25 lm a species forming a distinct lineage in Trypetheliaceae (Fig. 81). The genus Arthopyrenia sensu lato is polyphyletic with tropical, lichenized species clustering in Trypetheliaceae and a single, non-lichenized taxon, Arthopyrenia salicis, which falls in Pleosporales: there, A. salicis clusters with Roussoella and Roussoellopsis, and Liu et al. (2014) introduced Roussoellaceae to accommodate these taxa. Arthopyreniaceae was kept as a separate family in Pleosporales, based on its morphology and anatomy based on the type species, A. cerasi (Schrad.) A. Massal. which has not yet been sequenced. The new genus, Alloarthopyrenia is similar to other non-lichenized, temperate species formerly placed in Arthopyrenia sensu lato, including A. cerasi, in having a carbonaceous pseudoclypeus, branched and anastomosing pseudoparaphyses, bitunicate asci with shortpedicels, and hyaline ascospores (Coppins 1988; Hyde et al. 2013). Upreti and Pant (1993) illustrated species of Arthopyrenia from India. Alloarthopyrenia italica differs from Arthopyrenia cerasi in having depressed-globose to obpyriform ascostromata, wider hamathecium filaments (1.4 vs. 0.5–0.7 lm), obovoid to suboblong asci, and 1-septate, ovoid to ellipsoid ascospores; in contrast, A. cerasi has K ? greenish, larger and hemisphaerical ascostromata (300–500 lm), less branched and narrower pseudoparaphyses, cylindrical-clavate asci, and 3-septate, oblong ascospores (Coppins 1988; Nelsen et al. 2009; Hyde et al. 2013). It is therefore highly unlikely that the two species are congeneric and that A. cerasi also falls within Trypetheliaceae. Although the family Trypetheliaceae mainly comprises lichenized fungi, Nelsen et al. (2014) found some weakly or non-lichenized taxa in the family, forming basal lineages in the tree. This particularly applies to a non-lichenized, temperate species of Julella, and hence, the placement of a further, non-lichenized lineage basally in the family is not surprising. The internal anatomy of Alloarthopyrenia is otherwise typical of Trypetheliaceae, with the hamathecium forming a network of anastomosing hyphae embedded in a gelatinous matrix, bitunicate asci, transversely septate, and hyaline ascospores (Harris 1984; Prado et al. 2006; Nelsen et al. 2009). In the present study we introduce a new genus for this species in Trypetheliaceae, based on data from analysis of combined LSU and SSU sequence data. Alloarthopyrenia italica Phukhamsakda, Ariyawansa & K.D. Hyde, sp. nov. Camporesi, Index Fungorum number: IF552237; Facesoffungi number: FoF02380, Fig. 82 Etymology: Name reflects the country ‘‘Italy’’, where the holotype was collected. Holotype: MFLU 15-0399. Saprobic on living tree branches of Fraxinus ornus L. Thallus absent, algae not visible. Sexual morph Ascostromata 110–185 lm high 9 100–270 lm diam. ( x = 139 9 173 lm, n = 5), covered with a blackened pseudoclypeus, semi-immersed, with only ostioles visible, coriaceous, solitary, scattered or gregarious, depressed globose to obpyriform, wall rough, black to dark brown, flattened, ostiolate. Ostiole centrally located, oblong, filled with periphyses. Peridium 12– 23(–21 at apex) lm wide, composed of 5–6 layers of dark brown to light brown, cells of textura angularis and textura epidermoidea, easy to break, at base indistinguishable from host tissue. Hamathecium of numerous, dense, 1–1.8 lm wide ( x = 1.4 lm, n = 60), narrow, transversely septate, branched, anastomosing, trabeculate pseudoparaphyses, embedded in a gelatinous matrix. Asci 45–99 9 9–20 lm ( x = 66 9 15 lm, n = 30), 8-spored, bitunicate, fisitunicate, obviod to suboblong, short pedicellate, thick-walled, lightly faintly bluish in IKI ? , apically rounded, with ocular chamber up to 1–2 lm high. Ascospores 15–20 9 4–9 lm ( x = 18 9 7 lm, n = 50), partially overlapping or bi-seriate, hyaline, oviod or ellipsoid, slightly narrow at the apex when young, rounded at the apex when mature, 1-septate, strongly constricted at the septum, lower end tapering, wall rough, not uniform, smooth, indentations present when mature, surrounded by 2.5–4 lm wide, mucilaginous sheath. Asexual morph Undetermined. Material examined: ITALY, Forlı̀-Cesena Province, Monte Mirabello—Predappio, on dead branch of Fraxinus ornus L. (Oleaceae), 15 September 2014, E. Camporesi, IT 122 (MFLU 15-0399, holotype, isotype in HKAS 94615). Notes: Alloarthopyrenia italica was collected on living branches of Fraxinus ornus (Oleaceae) in Italy; it is nonlichenized and probably saprobic. Due to the taxon having similarities with Arthopyrenia, we compared its morphology with Arthopyrenia species reported from Fraxinus. Five species have been recorded from Fraxinus in Europe, including A. cerasi (Schrader) A. Massal (type species of Arthopyrenia, see above), A. cinereopruinosa (Schaerer) A. Massal., A. carneobrunneola B.J. Coppins, A. fraxini A. Massal., and A. ranunculospora B.J. Coppins & P. James (Coppins 1988; Index Fungorum 2016). Arthopyrenia carneobrunneola and A. ranunculospora differ in being lichenized (Coppins 1988). Arthopyrenia cinereopruinosa has been reported from Italy, but it is weakly lichenized and has larger ascostromata [(200–)300–440 lm] with 3-septate ascospores (Coppins 1988; Aptroot et al. 2008). Arthopyrenia fraxini differs in having larger ascostromata (250–500 lm), longer, clavate to cylindrical asci, and 123 136 Fungal Diversity (2016) 80:1–270 Fig. 92 Phylogram generated from maximum likelihood and Bayesian analyses based on ITS sequence data from species of Trichomeriaceae. The numbers above the nodes are RAxML bootstrap values expressed from 1000 repetitions with values above 50 % shown. The ex-type strains are in bold and the new isolates is in blue.The tree is rooted with Phaeococcomyces catenatus Trichomerium sp. MFUCC 13-0780 73 Trichomerium gleosporum MFLUCC 10-0087 Trichomerium foliicola MFLUCC 10-0078 100 73 Trichomerium foliicola MFLUCC 10-0073 Trichomerium sp. MFUCC 13-0789 Trichomerium foliicola MFLUCC 10-0054 90 Trichomerium foliicola MFLUCC 10-0058 99 Trichomerium siamensis MFUCC 12-0105 Trichomerium deniqulatum MFLUCC 10-0884 74 Chaetothyriales sp. CR07-3/2 95 81 Chaetothyriales sp CR08-2/1 100 100 93 Chaetothyriales sp CR08 -2/2 Trichomeriaceae Chaetothyriales sp. AL4 Chaetothyriales sp. M-Mo2 71 Chaetothyriales sp. CN-Cre-Bo3-2 93 Chaetothyriales sp. CN-Cre-Bo1-4 58 99 94 Chaetothyriales sp. CN-Phe1-1 Chaetothyriales sp. M-Camp4 67 Chaetothyriales sp. M-Cre2 Trichomerium bamboosicola MFUCC 13-0097 100 Chaetothyriales sp CN Cre Bo1 2 Chaetothyriales sp CR07 2 1 99 100 Chaetothyriales sp CR07 2 4 Chaetothyriales sp. CR07 3 1 Phaeococcomyces catenatus CBS 650.76 Herpotrichiellaceae 10 thick-walled, larger ascospores (20–30 9 4–9 lm), with blunt ends and lacking indentations (Coppins 1988; Upreti and Pant 1993). Our species is also similar to A. grisea (Schleich. ex Schaer.) Körb. but the latter has a yellow thallus, clavate to cylindrical uni-seriate asci, and 3-septate ascospores (Upreti and Pant 1993). As most Arthopyrenia species lack sequence data, their placement is uncertain. Pleosporales genera incetae sedis The following tree (Fig. 83) represents taxa which do not cluster in the main families of Dothideomycetes. Neomassarina Phookamsak, Jayasiri & K.D. Hyde, gen. nov. Index Fungorum number: IF552225; Facesoffungi number: FoF02259 Etymology: The generic epithet ‘‘Neomassarina’’ refers to the resemblance to Massarina 123 Saprobic on Agave angustifolia. Sexual morph Ascostromata black, solitary, scattered to clustered, immersed, erumpent through host surface, slightly raised, globose to subglobose, uni- to bi-loculate, glabrous, ostiole central, with a minute papilla. Peridium thin-walled, of unequal thickness, composed of 3–5 layers of dark pseudoparenchymatous cells, arranged in a textura angularis. Hamathecium composed of dense, 0.5–1 lm wide, cellular pseudoparaphyses, anastomosing among the asci, embedded in a hyaline gelatinous matrix. Asci 8-spored, bitunicate, fissitunicate, cylindrical to cylindric-clavate, short pedicellate. Ascospores overlapping uni- to bi-seriate, hyaline, pale brown at maturity, fusiform, 1-septate, constricted at the septum, smooth-walled with guttules, surrounded by a distinct mucilaginous sheath. Asexual morph Undetermined. Type species: Neomassarina thailandica Phookamsak, Jayasiri & K.D. Hyde Fungal Diversity (2016) 80:1–270 Fig. 93 Trichomerium bambusae (holotype). a Appearance of ascomata on leaf. b, c Section through ascoma. d Peridium at the base of ascoma. e Mycelium. f Ascomatal setae. g Apical ring. 137 h Ascus. i Ascus in Melzer’s reagent. k, l Ascospores. j Ascospore in Melzer’s reagent. Scale bars b = 100 lm, c, f = 50 lm, e, h, i = 20 lm d, g, k–l = 10 lm, f–i = 10 lm 123 138 Notes: Neomassarina is introduced as a monotypic genus to accommodate the massaina-like species, but not congeneric with Massarina. Neomassarina is similar to Massarina in having globose to subglobose ascostromata, with a thin-walled peridium and typically hyaline, fusiform ascospores, with broadly cellular pseudoparaphyses. However, the phylogeny indicates that they are distinct. Based on multi-gene phylogenetic analyses, Neomassarina form a single clade basal to Floricolaceae (Fig. 83). Therefore, the new genus is introduced in this study. Neomassarina thailandica Phookamsak, Jayasiri & K.D. Hyde, sp. nov. Index Fungorum number: IF552226; Facesoffungi number: FoF02260, Fig. 84 Etymology: The specific epithet ‘‘thailandica’’ refers to the country, from which the holotype was collected. Holotype: MFLU11-0144. Saprobic on dead bract-like leaves from flower stalk of Agave angustifolia Haw. Sexual morph Ascostromata 130–180 lm high, 100–200 lm diam., black, solitary, scattered to clustered, immersed, erumpent through host surface, slightly raised, with dark area around ostioles, globose to subglobose, uni- to bi-loculate, glabrous, ostiole central, with minute papilla. Peridium 7–16 lm wide, thinwalled, of unequal thickness, slightly thick at the apex, composed of 3–5 layers of flattened, dark brown to black, pseudoparenchymatous cells, arranged in a textura angularis. Hamathecium composed of dense, 0.5–1 lm wide, cellular pseudoparaphyses, distinctly septate, anastomosing among the asci, embedded in a hyaline gelatinous matrix. Asci (70–)75–90(–93) 9 7–8(–8.5) lm ( x = 81.8 9 7.6 lm, n = 20), 8-spored, bitunicate, fissitunicate, cylindrical to cylindric-clavate, short pedicellate, apically rounded, with an obtuse, ocular chamber. Ascospores (17.5–)18–20 9 3–4(–5) lm ( x = 18.6 9 4 lm, n = 25), overlapping uni- to bi-seriate, hyaline, pale brown at maturity, fusiform, 1-septate, constricted at the septum, Fig. 94 RAxML maximum likelihood phylogenetic tree based on LSU sequence data for species of Rhytismataceae. Maximum likelihood bootstrap values greater than 70 % are shown above the 123 Fungal Diversity (2016) 80:1–270 smooth-walled, guttulate, surrounded by a distinct mucilaginous sheath. Asexual morph Undetemined. Culture characteristics: Colonies on PDA reaching 20–25 mm diam. after 3 weeks at 25–30 °C; colony from above, white to cream; from below, white to cream at the margin, pale yellowish at the centre; medium dense, irregular, flattened, with undulate edge, surface slightly rough, cottony; not producing pigmentation in agar. Material examined: THAILAND, Chiang Mai, Muang District, Medicinal Plant Garden in Doi Suthep-Pui, on dead bract-like leaves from flower stalks of Agave angustifolia (Asparagaceae), 23 November 2009, R. Phookamsak, RP0015 (MFLU 11-0144, holotype), ex-type living cultures, MFLUCC 10-0552, BCC. Eurotiomycetes Eurotiales G.W. Martin ex Benny & Kimbr. Section Cremei Aspergillus P. Micheli ex Haller The genus Aspergillus (Trichocomaceae, Eurotiales) was established by Micheli (1729). The species belonging to this genus are characterized by their conidiophore patterns and the production of conidial heads (Samson et al. 2014). Species of Aspergillus are ubiquitous and are particularly abundant in the soil and in contaminated foods; about 339 species (Samson et al. 2014) are divided into four subgenera with 20 sections (Houbraken et al. 2014). About 60 species are known to be medically relevant pathogens, while many species are common contaminants as mycotoxins producers on various foods, others, such as A. oryzae, are important in commercial microbial fermentations as Koji mould. Currently, aspergillum is known as a name of an asexual spore-forming structure which is common to all species of Aspergillus (Dyer and O’Gorman 2011); about one-third of species are known to have a sexual morph. Among the sections of Aspergillus, section Cremei (known as A. cremeus group) was first described by Raper and Fennell (1965) for five species. Colonies of nodes. The new isolate is in blue, and other ex-type strains are in bold. The tree is rooted with Cudonia lutea Fungal Diversity (2016) 80:1–270 139 Fig. 95 Terriera thailandica (holotype). a, b Hysterothecia on host surface. c Hand section of hysterothecia. d Arrangement of asci. e Peridium. f–h Asci with ascospores. i, j Ascospores. Scale bars c = 50 lm, d = 50 lm, e, f = 20 lm, g–h = 30 lm, i–j = 20 lm 123 140 Fungal Diversity (2016) 80:1–270 Fig. 96 Phylogenetic tree inferred from most likelihood (ML) analysis and Bayesian inference (BI) using combined ITS and LSU data (ln L = 2297.425977). Only the topology generated from the ML analysis is shown. Bayesian Posterior Probability [0.95 are indicated with thick branch. New taxa are in blue species belonging to this section are characterized by shades of yellowish-brown to brown or grey-green, with biseriate conidial heads and long conidiophores (Samson et al. 2014). Their conidia are pale grey-green to yellowbrown (Peterson 1995). Among species assigned to this section, A. wentii is known as a source of enzymes (Raper and Fennell 1965; Lowe 1992) and A. inflatus is reported to 123 produce sterigmatocystin—a precursor to the even more potent compounds, the aflatoxins (Rank et al. 2011). During the investigation of the fungi from rhizosphere soil of pine trees in forests of Gunsan, Korea in August 2015, an interesting species of Aspergillus was discovered. It proved to be sufficiently different from previously described species (Stolk and Malla 1971; Samson et al. 2014) to warrant Fungal Diversity (2016) 80:1–270 141 Fig. 97 Helvella tinta (holotype). a Typical mature specimens. b Receptacle surface of pileus. c, d Asci and paraphyses. e–g Asci with ascospores. Scale bars a = 1 cm, b = 50 lm, c–g = 20 lm 123 142 description as a new species, forming a distinct lineage. Previously only one species, A. cibarius from traditional Meju, has been reported as a new species in Korea (Hong et al. 2012). Aspergillus koreanus Hyang B. Lee, T.T. Duong & T.T.T. Nguyen, sp. nov. MycoBank number: MB816938; Facesoffungi number: FoF02476, Fig. 88 Etymology: koreanus, referring to the country from which the species was first isolated (Korea) Holotype: EML-GSNP1-1 Colonies on MEA reaching 41–44 mm diam. at 25 °C in 7 days, initially white to white-cotton, turning greyishbrown in the center with age and the abundant sporulation; reverse white to pale yellowish. Conidiophores hyaline, smooth, septate, varying greatly in length, 4.5–6 lm diam. Vesicles subglobose to globose, 7.5–12 lm diam. Conidial heads bi-seriate, consisting of many metulae bearing several phialides. Phialides ampulliform, 6–9 9 1.5–2.5 lm, extending from metulae. Conidia grey-green, slightly roughened, borne in short or long chains, subglobose to globose, 2.5–3.5 lm diam. Cleistothecia not observed. Notes: Aspergillus koreanus is distinct from A. inflatus, growing rapidly when cultivated on MEA producing subglobose to globose vesicles successively and bearing metulae which develop simultaneously. The metulae consist of several (commonly 3) phialides. The conidial colour is consistently grey-green with a thin band in the middle to connect conidia into a chain, while that of A. inflatus is brownish, with mostly two roughly parallel very thin bands. A part from phylogenetic characterization; phylogenetic comparisons from single and multi-gene sequence analyses clearly indicate that A. koreanus strains are related to A. inflatus and represent a new taxon (Figs. 85, 86, 87). Material examined: REPUBLIC OF KOREA, Jeonnam Province, Gunsan City, Sinsido Island (35.82°N, 126.45°E), from a rhizosphere soil of pine tree in forest, 7 August 2015; EML-GSNP1-1 holotype, (ex-type) at Culture Collection of National Institute of Biological Resources (NIBR), Incheon, and preserved as glycerol stock at -80 °C in the Chonnam National University Fungal Collection (CNUFC); living culture (ex-type) deposited at Jena Microbial Resource Collection (University of Jena and Leibniz Institute for Natural Product Research and Infection Biology, Jena, Germany) (JMRC:SF:012334). The isolate was observed to grow over a wide range of temperatures, with varying growth rates The average growth rates on MEA, PDA, YES, OA, CYA, and CREA were 6, 5.5, 4.5, 4, 3, and 0.2 mm per day, respectively at 25 °C. Optimal growth was observed at 25 °C, slow growth was observed at below 20 °C and no growth at 35 °C (Fig. 88). 123 Fungal Diversity (2016) 80:1–270 Chaetothyriales M.E. Barr Chaetothyriales comprises Chaetothyriaceae, Cyphellophoraceae and Herpotrichiellaceae based on molecular analysis (Réblová et al. 2013), and includes the sooty mould families Chaetothyriaceae, Coccodiniaceae and Trichomeriaceae (Chomnunti et al. 2012b, 2014). Chaetothyriaceae Hansf. ex M.E. Barr For the family Chaetothyriaceae, we follow Chomnunti et al. (2014). Ceramothyrium menglunense Mapook, J.F. Li & K.D. Hyde, sp. nov. Index Fungorum number: IF552275, Facesoffungi number: FoF02477, Fig. 90 Etymology: Named for the town, Menglun, where the holotype was collected. Holotype: MFLU 16-1874 Saprobic on a dead leaves of Syzygium sp. Sexual morph Ascomata (205–)245–255(–370) lm high 9 140– 165 lm diam. ( x = 270 9 150 lm, n = 5), superficial, solitary or scattered, coriaceous, globose to subglobose, dark brown to black with scattered brown setae, Ostiole central, minutely papillate. Peridium (10–)20–60 lm wide, 2-layered, outer layer comprising dark brown to black cells of textura globulosa, inner layer comprising light brown to hyaline cells of textura angularis. Hamathecium comprising 2–3 lm wide, cylindrical to filiform, septate, branched, pseudoparaphyses. Asci 50–75 9 24–30(–37) lm ( x = 65 9 28 lm, n = 10), 8-spored, bitunicate, clavate to pyriform, short pedicellate, with an ocular chamber. Ascospores 25–35 9 10–12 lm ( x = 28 9 11 lm, n = 10), overlapping, hyaline, ellipsoid to obovoid, muriform, with 4–7 transverse septa, and 1–5 vertical septa when mature, constricted at the septa, smooth-walled, without mucilaginous sheath. Asexual morph Undetermined. Material examined: CHINA, Yunnan Province, Xishuangbanna, Menglun, on dead leaves, 13 February 2015, J.F. Li (MFLU 16-1874, holotype), ex-type culture MFLUCC 14-1120, (isotype in HKAS, under the code of HKAS 95077). Notes: Maximum likelihood (RAxML) and Bayesian analyses of combined LSU and ITS sequences data showed that our collection is closely related to Ceramothyrium thailandicum. However, our collection is morphologically distinct in having larger ascomata with brown setae, shorter and narrow asci and wider ascospores with vertical septa. Thus, based on morphology and phylogeny support, C. menglunense is a new species (Figs. 89, 90). Herpotrichiellaceae Munk The family Herpotrichiellaceae was established by Munk and encompasses loculoascomycetes with small, Fungal Diversity (2016) 80:1–270 143 Fig. 98 Helvella floriforma (holotype). a Typical mature specimens. b Receptacle surface of pileus. c Stipitipellis. d Asci and paraphyses. e, f Asci. g Ascospores. Scalebars a = 1 cm, b–c = 50 lm, d–g = 20 lm superficial, inconspicuous, setose ascomata, bitunicate asci with a thickened endotunica and greenish-grey to brown, septate ascospores (Munk 1953). Barr (1976) included the family Herpotrichiellaceae in the order Chaetothyriales. Phylogenetic analysis also supported the family Herpotrichiellaceae as belonging to the order Chaetothyriales (Liu et al. 2015b). Minimelanolocus R.F. Castañeda & Heredia Castañeda-Ruiz et al. (2001) introduced the genus Minimelanolocus with M. navicularis (R.F. Castañeda) R.F. Castaneda as the type species. They transferred ten species from the genera Pseudospiropes, Helminthosporium, and Belemnospora into Minimelanolocus, and described one new species, M. curvisporus. Several species of Minimelanolocus have since been described from a wide range of hosts worldwide based on morphology (Zhang et al. 2010; Ma et al. 2011a, b; Hernández-Restrepo et al. 2013; Xia et al. 2014; Liu et al. 2015b). Liu et al. (2015b) listed 28 species of Minimelanolocus and provided the morphological characters of these taxa. A phylogenetic tree of Chaetothyriales, based on combined LSU and ITS sequence data, is provided in this paper (Fig. 89). Minimelanolocus submersus Z.L. Luo, H.Y. Su & K.D. Hyde, sp. nov. Index Fungorum number: IF552154, Facesoffungi number: FoF02219, Fig. 91 123 144 Fungal Diversity (2016) 80:1–270 Fig. 99 Helvella oblongispora. a–c Typical mature specimens (a HKAS 87693, b HKAS 87726, c HKAS 87694). d Receptacle surface of pileus. e, f Stipitipellis. g, h Asci and paraphyses. i–k Asci l. Ascospores. Scale bars a–c = 1 cm, d–f = 70 lm, g–l = 20 lm Etymology: With reference to the submerged habitat. Holotype: HKAS 92593. Saprobic on submerged decaying wood. Sexual morph Undetermined. Asexual morph Colonies on the substrate superficial, effuse, hairy, scattered, brown. Mycelium mostly immersed, composed of septate, dark brown, smooth hyphae. Conidiophores mononematous, macronematous, unbranched, erect, straight or slightly flexuous, 123 smooth, cylindrical, septate, dark brown, gradually paler towards the apex, 110–180 lm ( x = 145.5 lm, SD = 35.5 lm, n = 10) long, 5–6 lm ( x = 5.5 lm, SD = 0.5 lm, n = 10) wide. Conidiogenous cells holoblastic, integrated, sympodially proliferating, terminal, pale brown or subhyaline. Conidia acrogenous, clavate to fusiform, solitary, immature conidia 1-septate, mostly 2–5septate at maturity, dry, subhyaline to pale brown, 19– Fungal Diversity (2016) 80:1–270 33 lm ( x = 26 lm, SD = 7 lm, n = 20) long, 3.5– 4.5 lm ( x = 4 lm, SD = 0.5 lm, n = 20) wide. Conidial secession schizolytic. Culture characteristics: Colonies on PDA 25 mm diam. after 3 weeks at room temperature, pale brown at the margins, dark brown at the center; reverse steel grey, medium dense, circular, fairly tight. Material examined: CHINA, Yunnan Province, Dali, Erhai Lake, saprobic on submerged decaying wood, June 2015, X.Y. Liu, S-323 (HKAS 92593, holotype), ex-type culture KUMCC 15-0206, MFLUCC. Notes: In the phylogenetic analysis, Minimelanolocus submersus clustered with species of Minimelanolocus; and formed a sister group to M. obscurus (Matsush.) R.F. Castañeda & Heredia. M. submersus closely resembles M. aquaticus H.Y. Su etal. and M. asiaticus H.Y. Su et al. but differs from M. aquaticus in having 2–5-septate, narrower conidia (3.5–4.5 lm vs. 4–8 lm), and differs from M. asiaticus in having longer and wider conidiophores (110–181 9 5–6 lm vs. 112–162 9 3.5–4.5 lm) (Liu et al. 2015b). Trichomeriaceae Chomnunti & K.D. Hyde The family was introduced and placed in Chaetothyriales by Chomnunti et al. (2012a), with the generic type Trichomerium. Trichomeriaceae comprises several species of sooty moulds. The family presently comprises three genera, Bradymyces, Knufia, and Trichomerium (Hubka et al. 2014). Trichomeriaceae includes strains that were isolated from the surface of rocks as well as fungi associated with ants (Hubka et al. 2014). In this study, we introduce a new species isolated from a plant, based on morphology and phylogenetic analysis. We provide a tree for Trichomeriaceae below (Fig. 92). Trichomerium Lév. Trichomerium species differ from Capnodiaceae species in having loose mycelium beneath the ascomata, the appearance of ascomatal setae, and fusoid ascospores, with three transverse septa or sometimes with longitudinal septa. Phylogenetic analyses from previous studies indicate that Trichomeriaceae species belong to Eurotiomycetes, and Capnodiaceae belongs to Dothideomycetes. Trichomerium bambusae Hongsanan & K.D. Hyde, sp. nov. Index Fungorum number: IF552365; Facesoffungi number: FoF02446, Fig. 93 Etymology: Named after the host bamboo. Holotype: MFLU 16-2286 Epiphytes, saprobic on the surface of culm of bamboo. Superficial hyphae 5–6 lm wide, branched, septate, constricted at the septa, brown. Sexual morph Ascomata 140– 145 180 high 9 105–130 wide lm ( x = 154 9 118 lm, n = 7), superficial, solitary to gregarious, mainly globose to subglobose, with long ostiole at the center, held to the leaf surface by basal mycelium, brown to greyish, with apical setae. Setae 50–80 9 5–8 lm ( x = 65 9 7.5 lm, n = 10), surrounding the ascomata, usually at the upper part of ascomata, dark brown to black, slightly narrower and paler at the apex, straight, dark brown to black. Peridium 13–17 lm ( x = 14 lm, n = 10), comprising two layers, outer layer composed of pigmented, thick-walled cells of textura angularis, inner layer composed of pale, flattened cells of textura angularis. Hamathecium not observed. Asci 53–62 9 19–24 lm ( x = 57 9 22 lm, n = 10), 8-spored, bitunicate, broadly cylindrical or oblong, short pedicellate, ocular chamber when immature. Ascospores 15–18 9 5–7 lm ( x = 17 9 6 lm, n = 15), 2–3-seriate, oblong, hyaline, 3-septate, usually not constricted at the septa, but sometimes slightly constricted at the lowest septum, with thin mucilaginous sheath at the middle, smooth-walled, end cells narrow and smaller than central cell. Asexual morph Undetermined. Material examined: THAILAND, Chiang Rai, Bandoo, on dead of culm of bamboo (Poaceae), December 2012, SC Karunarathna HSA59 (MFLU 16-2286, holotype; isotype in KIB); ex-type living culture, MFLUCC 13-0097. Notes: Trichomerium bambusae differs from other species within Trichomerium in having a long central ostiole, and 2–3-septate ascospores, with very thin mucilaginous sheath. The new species is most similar to T. gloeosporum Chomnunti & K.D. Hyde based on the shape of ascomata, with raised ostioles, however, T. bambusae has a longer ostioles than T. gloeosporum. The ascospores of T. gloeosporum have a thick mucilaginous sheath, while its mucilaginous sheath is poorly developed in T. bambusae. In the phylogenetic analysis T. bambusae clusters with strains of Chaetothyriales isolated from carton fungi (fungi associated on ant carton nests) and other Trichomerium species. Ant fungi are a polyphyletic group which also present in Capnodiales (Voglmayr et al. 2010). Leotiomycetes We follow Wang et al. (2006) for the classification of this order. Rhytismatales M.E. Barr ex Minter Rhytismatales is an order of endophytic, parasitic or saprotrophic fungi in the class Leotiomycetes (Ascomycota), the inoperculate discomycetes. Four families are currently recognized in the order. Rhytismataceae Chevall. Rhytismataceae is largest family (Johnston 2001) and includes 44 genera (Lumbsch and Huhndorf 2010), 123 146 Fungal Diversity (2016) 80:1–270 Peziza vesiculosa DQ491509 Peziza vesiculosa JF908568 Peziza vesiculosa AF491625 Peziza vesiculosa AF491623 Peziza bovina JF908555 1/100 1/100 Peziza fimeti JF908537 Peziza sp. UDB015563 0.89/63 Peziza domiciliana JF908561 Peziza granularis JF908558 0.98/75 1/100 Peziza ammophila JX845423 Peziza ammophila JX845421 1/100 Peziza pseudoammophila JF908559 Peziza ammophila AF491622 Peziza ammophila AF491621 0.73/Peziza ammophila UDB019674 1/97 1/100 Peziza ammophila UDB019673 1/91 Peziza varia AF491553 Peziza varia AF491551 0.87/54 Peziza varia AF491550 Peziza varia AY789392 1/100 Peziza varia AF491549 1/82 Peziza varia AF491548 1/100 Peziza varia AF491558 Peziza varia AF491557 1/ 100 Peziza varia AF491556 1/81 Peziza fimeti JQ654490 Peziza varia AF491555 1/100 Peziza varia JF908557 1/99 Peziza varia AF491570 Peziza varia AF491569 Peziza varia AF491568 Peziza varia UDB018603 0.99/93 0.99/88 1/100 0.95/- 1/98 1/100 0.96/75 1/71 Peziza sp. JX415342 Peziza echinospora JF908533 Peziza echinospora AF491575 Peziza echinospora AF491573 Peziza sp. AF491572 1/100 Peziza sp. AF491571 1/100 0.99/77 Peziza varia JF908560 Peziza sp. AF491587 Peziza sp. AF491586 0.88/53 Peziza arvernensis JF908569 1/96 Peziza arvernensis AF491577 Peziza arvernensis AF491580 Peziza fimeti JQ654489 1/100 Peziza nivalis JX845425 Peziza nivalis AF491620 Peziza nivalis AF491619 1/100 Peziza fruticosa AMB 17136 Calambrone 1/69 Peziza fruticosa AMB 17135 Rosolina 1/55 0.86/Peziza ninguis JF908536 0.99 Peziza domiciliana AF491614 /100 Peziza perdicina JF908529 0.87/54 Peziza ampliata AF491590 Peziza ampliata AF491592 1/Peziza fimeti JQ654491 1/100 Peziza fimeti JQ654487 0.75/73 Peziza fimeti AF491605 Peziza fimeti JQ654495 Peziza fimeti JQ654493 Peziza fimeti AF491600 Peziza udicola JF908549 Peziza lohjaoensis AF491576 1/99 Peziza proteana JF908566 1/91 Peziza proteana DQ491497 Peziza petersii JF908550 1/100 0.76/54 Peziza petersii JF908527 Peziza petersii AF133179 Peziza fruticosa 1/100 Peziza michelii JN836748 Peziza michelii DQ200839 Peziza subcitrina AF491627 0.1 expected changes per site Fig. 100 Phylogeny of Peziza based on a Bayesian and maximum likelihood analysis of ITS dataset. Bayesian posterior probability (BPP) values (in bold) C0.7 and maximum likelihood bootstrap (MLB) values C50 % are shown on the branches. Thickened branches indicate BPP C 0.95 and MLB support C70 %. Peziza subcitrina was chosen as outgroup taxon Lophodermium is the largest genus with more than 100 species currently accepted (Lantz et al. 2011). Etymology: thailandica is based on country where species was found. Holotype: MFLU 16-0945. Saprobic on decaying woody branches in terrestrial habitats, associated with bleached, pale brown areas. Sexual morph Ascomata hysterothecial, 1100–1900(–900) long 9 222–250 high, 210–268 lm diam., in surface view, matt, elliptical, ends rounded to subacute, margins diffuse, the central part of the ascomata strongly raising the surface of the substrate at maturity, opening by a longitudinal split that extends almost the whole length of the ascoma. Peridium 17–44 lm, carbonaceous, brittle, of heavily pigmented, basal cells of textura angularis and globulosa, covering stroma consisting of an outer layer of host cuticle. Hamathecium comprising 1–2 lm wide, hyaline, aseptate, excipulum moderately developed, borne in a gel matrix, closely adhering to the covering stroma and the extension, arising from the marginal paraphyses. Asci 80–105 9 3.4– Terriera B. Erikss. The genus Terriera is a member of Rhytismataceae (Kirk et al. 2008). The type species is T. cladophila (Lév. in Moug. & Nestl.) B. Erikss (syn. Lophodermium cladophilum (Lév. In Moug. & Nestl.) Rehm) (Eriksson 1970). Johnston (1988, 1989) enumerated some important features for delimitation of this genus, such as oblong to sublinear ascomata, the lack of lip cells, covering stroma forming a platform in vertical section, and the triangular space in section between the covering stroma and basal stroma filled with vertically oriented cells. Terriera thailandica Jayasiri & K.D. Hyde, sp. nov. Index Fungorum number: IF552171; Facesoffungi number: FoF02222, Fig. 95 123 Fungal Diversity (2016) 80:1–270 147 Peziza exogelatinosa AY500501 0.85/ - 1.0/100 Peziza quelepidotia AF107809 Peziza natrophila AY500486 0.93/52 Peziza sp. PM 120 97 AY500488 0.95/54 Peziza subcitrina AY500520 Peziza howsei AY500493 0.98/ - 1.0/100 0.82/ - Peziza emileia KJ728718 Peziza emileia KJ728719 0.99/54 Peziza lobulata AY500495 Peziza ampelina AY500492 1.0/100 Peziza vesiculosa DQ470898 Peziza vesiculosa AY500489 Peziza varia AY500498 1.0/100 Peziza varia AY500499 0.98/57 Peziza varia AY500519 0.96/53 1.0/96 Peziza varia AY500500 0.99/70 0.82/58 Peziza echinospora AY500496 1.0/85 Peziza arvernensis LN714689 Peziza arvernensis AY500497 1.0/100 Peziza ampliata AY500510 Peziza fruticosa 0.99/100 Peziza polaripapulata AY500514 Peziza polaripapulata AY500515 0.05 expected changes per site Peziza obtuspiculata AY500490 Fig. 101 Phylogeny of Peziza based on a Bayesian and maximum likelihood analysis of RPB2 dataset. Bayesian posterior probability (BPP) values (in bold) C0.7 and maximum likelihood bootstrap (MLB) values C50 % are shown on the branches. Thickened branches indicate BPP C 0.95 and MLB support C70 %. Newly sequence collections are in bold. Peziza polaripapulata and P. obtuspiculata are chosen as the outgroup taxa 6.6 lm ( x = 90 9 5 lm, n = 15), 8-spored, crowded to somewhat parallel, cylindrical, but cylindrical-clavate when immature, apex obtuse to truncate, thin-walled, without a circumapical thickening, long stalked J-, discharging spores through a small apical pore. Ascospores 38–60 9 1–1.5 lm ( x = 45 9 1.2 lm, n = 25), arranged in a fascicle, hyaline, filiform, tapering slightly towards both ends, aseptate, pluriguttulate, cell wall not clear, without a gelatinous sheath or appendages. Asexual morph Undetermined. Culture characters: Colonies on MEA 25 mm diam. after 7 days at 25 °C, raised, with lobate margin; colony two layered, outer layer off white and bound to media, inner layer white and finely floccose to woolly aerial mycelia. Reverse white with black colour patches, prominent in middle. Material examined: THAILAND. Chiang Rai, Doi Pui: dead branch of undetermined tree, 15 June 2014, Subashini C. Jayasiri. (MFLU 16-0945, holotype), (isotype in KUN), ex-type culture, MFLUCC 14-0818, KUNCC. Notes: Terriera thailandica is introduced here based on both morphology and phylogeny. Terriera was segregated from Lophodermium based mainly on its swollen paraphyses at the apex to form an epithecium and the lack of lip cells (Eriksson 1970). Terriera thailandica shares these characters with other species of the genus. Terriera thailandica clusters with T. camelliicola in a clade with high ML support and distinct from T. thailandica and T. minor (Fig. 94). Terriera thailandica shares similar morphological characters with T. camelliicola, but the latter species has hysterothecia which are more or less curved, lacks an 123 148 123 Fungal Diversity (2016) 80:1–270 Fungal Diversity (2016) 80:1–270 b Fig. 102 Peziza fruticosa (holotype). a, b Ascospores in Lactic blue. c–e Ascospores in Melzer’s reagent. f Hymenium in water. g Ascus and paraphyses in Melzer’s reagent. h Asci and paraphyses in water. i Apothecia in situ. Scale bars a = 8 lm, b = 4 lm, c = 3 lm, d, e = 2.5 lm, f = 30 lm, g, h = 15 lm, i = 2 mm (Photo G. Medardi) epithecium, and has observable conidiomata near the hysterothecia (Zhang et al. 2015). Pezizomycetes Pezizales J. Schrot. The order Pezizales has long attracted the attention of mycologists from different parts of the world. Currently, there are 16 families, namely, Ascobolaceae, Ascodesmidaceae, Caloscyphaceae, Carbomycetaceae, Chorioactidaceae, Discinaceae, Glaziellaceae, Helvellaceae, Karstenellaceae, Morchellaceae, Pezizaceae, Pyronemataceae, Rhizinaceae, Sarcoscyphaceae, Sarcosomataceae and Tuberaceae in the order (Hibbett et al. 2007; Kirk et al. 2008). This order is distinguished by asci generally open by rupturing to form a terminal or eccentric lid or operculum (Hansen and Pfister 2006). Helvellaceae Fr. This family was introduced by Fries (1822) and is widely distributed in temperate to arctic-alpine areas (e.g. Abbott and Currah 1997; Dissing 1966; Ying and Zang 149 1994; Zhuang 2004), with a few taxa known from the tropics (Dissing 1979). To date, the family includes four genera, viz. Balsamia., Barssia, Helvella and Wynnella, that comprise approximately 100 species (Kirk et al. 2008; Nguyen et al. 2013; Zhao et al. 2015b, 2016a, b; Wang et al. 2016). Helvella L. Helvella is the type genus of Helvellaceae, is characterized by its epigeous, stipitate, cupulate, saddle-shaped to irregularly-lobed ascomata, with whitish, cream, greyish, brown to black hymenium, glabrous, pubescent to villose receptacle surface, and terete or externally sulcate, solid, hollow or lacunose stipe (Dissing 1966; Abbott and Currah 1997). This genus has received much attention in China, Europe and North America, in the last decade (Zhuang 2004; Zhuang and Yang 2008; Ariyawansa et al. 2015a; Hwang et al. 2015; Zhao et al. 2015b, 2016a, b; Wang et al. 2016). To date, 20 species have been described from China (Wang et al. 2016). In this study we introduced two new species based on molecular support (Fig. 96) and a new record for Helvella oblongispora. Helvella tinta Q. Zhao, B. Feng & K.D. Hyde, sp. nov. Index Fungorum number: IF552355, Facesoffungi number: FoF02447, Fig. 97 Etymology: Named because of its tints hymenium and receptacle surface. Fig. 103 Diagrammatic representation of the microscopic characters of Peziza fruticosa (holotype). a Vertical section of one apothecium. a1 Hymenium. a2 Subhymenium. a3 Upper medullar excipulum. a4 Median medullar excipulum. a5 Lower medullar excipulum. a6 Ectal excipulum. b Ascospores. c Asci and ascospores. d Paraphyses. Scale bars a = 100 lm, b = 10 lm, c, d = 25 lm (drawing by Del. G. Medardi) 123 150 Fungal Diversity (2016) 80:1–270 Fig. 104 Coronophora myricoides (holotype). a Herbarium material. b, c Appearance of ascomata on wood. d, e Vertical sections of ascomata. f Peridium. g–i Asci. j–l Ascospores. Scale bars c = 200 lm, d = 50 lm, e, g, h = 20 lm, f, i–l = 10 lm Holotype: HKAS 82560. Symbiotic in coniferous forests of Picea euphratica Oliv. Sexual morph Pileus cupulate to irregularly cupulate, 2–3 cm high, 1.5–3.5 cm broad, margin slightly flattened; hymenium glabrous, even, brown to dark brown, usually mottled with patches of paler pigmentation, greyish to dark brown when fresh, blackish-brown when dried; receptacle surface pubescent, concolourous with the hymenium, ribs absent or extending onto basal quarter only. Stipe 2.5–4 cm long, 0.7–1.5 cm broad, glabrous, flaring and 123 merging with apothecium, white to smoky grey, becoming cream and tough when dried, with 6–8 ribs, with roundededge, few anastomosis between ribs, basal mycelium white. Medullary excipulum 370–500 lm broad, of textura intricata, hyaline, composed of 4–6 lm broad hyphae, J-. Ectal excipulum 70–180 lm broad, of textura angularis, outermost cells catenuliform in long fascicled tufts, 14–38 9 8–18 lm, brown, evenly blue in cotton blue, J?. Asci 270–350 9 14–20 lm, pleurorhynchous base, 8-spored, uniseriate, subcylindrical to clavate. Paraphyses filiform, Fungal Diversity (2016) 80:1–270 151 Fig. 105 One of 255 most parsimonious trees obtained from analyses of combined ITS, EF-1a, b-tubulin and CAL sequence data for all ex-types from species in Diaporthe. Isolate numbers of new species and new host records are in blue. Maximum parsimony bootstrap values ([70 %) and Bayesian inference values ([0.8) are given on the nodes. The tree is rooted to Diaporthe corylina 123 152 Fungal Diversity (2016) 80:1–270 Fig. 105 continued 3–5 lm broad, slightly exceeding the asci, light brown, with a slightly yellow refractive content in Melzer’s reagent, blue in cotton blue, apex slightly enlarged, 4–5.5 lm broad. 123 Ascospores [30/2/2, in H2O] (15–)16–19(–20) 9 10–13(–14) lm [Q = 1.36–1.77, Q = 1.59 ± 0.09)], ellipsoid, smoothwalled. All tissues J-. Asexual morph Undetermined. Fungal Diversity (2016) 80:1–270 153 Fig. 106 Diaporthe aseana (MFLU 13-0256, holotype). a Specimen on dead leaf. b Conidiomata on the host surface. c Longitudinal section of a conidioma. d, e Longitudinal section of a conidioma wall. f, g Conidiogenous cells with developing conidia. h–k Conidia. l Germinating conidium. m, n Colonies on PDA, m from above n from below. Scale bars c = 100 lm, d = 50 lm, e–l = 10 lm 123 154 Habitat and distribution: Scattered or gregarious on the moss, under Picea euphratica Oliv., forests. Known only from southwestern China. Material examined: CHINA, Sichuan Province, Hongyuan County, alt. 3300 m, 12 August 2013, Bang Feng 1454 (HKAS 82560, holotype). Notes: Helvella tinta is well characterized by its cupulate to irregularly cupulate pileus with a consistently mottled, greyish to dark brown hymenium, and a lacunose stipe possessing ribs extending onto 1/4 of the receptacle surface. Paraphyses light brown, 3–5 lm. The brown pigments of the receptacle surface are visible in cotton blue or Melzer’s reagent. Morphologically, H. tinta is similar to H. maculata N.S. Weber, a species originally described from North America, but the latter has a saddle-shaped pileus with a drab, buffy brown to snuff brown hymenium, margin curved to receptacle surface, a white to cream stipe and large ascospores (Weber 1975). Helevlla floriforma also shares some features with H. tinta, but it has a peculiar apothecial shape and colour. Phylogenetically, H. tinta clusters with H. griseoalba N.S. Weber (66 % bootstrap support). However, H. griseoalba differs from H. tinta in having grey to cinnamon hymenium and receptacle surface and hyaline paraphyses (Weber 1972; Landeros et al. 2012). Helvella floriforma Q. Zhao & K.D. Hyde, sp. nov. Index Fungorum number: IF552356, Facesoffungi number: FoF02448, Fig. 98 Etymology: the epithet refers to the flower-shaped apothecia. Holotype: HKAS 90224. Symbiotic in the coniferous forests of Platycladus orientalis (L.) Franco. Sexual morph Pileus slightly infundibuliform to central strongly depressed but not hollow when mature, 2–3 cm high, 2–6 cm broad, margin rolled to receptacle surface, wavy; hymenium glabrous, drab, buffy brown to snuff brown when fresh, yellowishbrown when dried; receptacle surface pubescent, cream, pale, apricot greyish when fresh, becoming yellowish when dried, branching and anastomosing ribs extending to marginal region. Stipe 2–4 cm long, 1.5–3 cm broad, cream, becoming yellowish when dried, glabrous, sulcate, branched and anastomosed ribs extending to the receptacle surface margin. Medullary excipulum 290–450 lm broad, of textura intricata, hyaline, composed of 3–5 lm broad hyphae, with a red refractive content in Melzer’s reagent, blue in cotton blue, J-. Ectal excipulum 100–130 lm broad, of textura angularis, outermost cells catenuliform in long fascicled tufts, hyaline, evenly blue in cotton blue, cylindrical end cells 13–25 9 7–13 lm, J?. Stipitipellis 50–70 lm, hyaline, composed of textura angularis, 123 Fungal Diversity (2016) 80:1–270 Fig. 107 Diaporthe eres (MFLUCC 12-0351). a Specimen on dead c cone of Picea excelsa. b Conidiomata on the host surface. c Longitudinal section of a conidiomata. d–g Longitudinal section of peridium. h–m Conidiogenous cells with developing conidia. n– s Conidiogenous cells with developing conidia stained with lactophenol cotton blue. t Conidia. u Conidia stained with lactophenol cotton blue. v Germinating conidium. w–x Colonies on PDA, w from top, x from reverse. Scale bars c = 100 lm; d–g = 50 lm; h–u = 5 lm; v = 10 lm terminal cells 13–27 9 7–10 lm, clavate, with a yellow refractive content in Melzer’s reagent, blue in cotton blue, J-. Asci 260–310 9 16–22.5 lm, pleurorhynchous base, 8-spored, uni-seriate, subcylindrical to clavate. Paraphyses filiform, 3–4 lm broad, slightly exceeding the asci, with a slightly yellow refractive content in Melzer’s reagent, blue in cotton blue, apex 4–5 lm broad, J-. Ascospores [40/2/1, in H2O] (14)14.5–20 9 10.5–16 lm [Q = 1.16–1.59, Q = 1.34 ± 0.11)], subglobose to subellipsoid, smoothwalled. Asexual morph Undetermined. Habitat and known distribution: Scattered or gregarious on the ground, under Platycladus orientalis. Currently known only in southwestern China. Material examined: CHINA, Yunnan Province, Gucheng County, alt. 2500 m, 19 August 2013, Qi Zhao 2000 (HKAS 90224, holotype). Notes: Helvella floriforma is characterized by its slightly infundibuliform to strongly depressed pileus with a wavy, curved to receptacle surface margin, a glabrous, drab, buffy brown to snuff brown hymenium, and a pubescent, cream, pale, apricot greyish, ribs branching and anastomosing extending to marginal region receptacle surface. Stipe cream, glabrous, sulcate, rounded-edge, branched and anastomosed ribs extending to the receptacle surface margin. Stipe inner and ectal excipulum J?. In our phylogenetic analysis, H. floriforma is sister to H. robusta S.P. Abbott with relatively high statistical support (91 %) (Fig. 96). However, the latter species is irregularly cupulate with a large central depression to irregularly bi-lobed pileus, free margin, a subpubescent to pubescent, ribbed receptacle surface and a subpubescent to pubescent, lacunose stipe with sharp ribs; medullary excipulum and stipe inner and stipitipellis J?; ectal excipulum J- (Landeros et al. 2012). Helvella tinta and H. maculata also have mottled hymenium surface and lacunose stipe. However, H. tinta has a cupulate to irregularly cupulate pileus, with a greyish to dark brown hymenium, a mottled receptacle surface only near the stipe possessing ribs, all tissues J-. Helvella maculata has a saddle-shaped pileus, margin rolled or straight toward the hymenium and a white to cream stipe, all tissues J- (Weber 1975; Abbott and Currah 1997; Landeros et al. 2012). Fungal Diversity (2016) 80:1–270 155 123 156 123 Fungal Diversity (2016) 80:1–270 Fungal Diversity (2016) 80:1–270 b Fig. 108 Diaporthe foeniculina (MFLUCC 12-0668). a Specimen on dead branch of Laburnum sp. b Conidiomata on the host surface. c, d Longitudinal section of a conidiomata. e, f Longitudinal section of peridium. g–l developing conidia. m–p Developing conidia stained with lactophenol cotton blue. q Alpha conidia. r Alpha conidia stained with lactophenol cotton blue. s Beta conidia. t Beta conidia stained with lactophenol cotton blue. u Germinating conidium. v, w Colonies on PDA, v from top, w from reverse. Scale bars c, d = 100 lm; e, f = 50 lm; g–t = 5 lm; u = 10 lm Helvella oblongispora Harmaja, Karstenia 18(2): 57 (1978) Facesoffungi number: FoF02449, Fig. 99 Symbiotic in the coniferous forests of Picea euphratica Oliv. Sexual morph Pileus cupulate to irregularly cupulate, 0.5–2.5 cm high, 0.5–2.5 cm broad, margin slightly splitting; hymenium glabrous, even to slightly undulate at center, greyish-brown to brown when fresh, blackishbrown when dried; receptacle surface finely pubescent, greyish to brown at the margin, cream to white below, white near stipe, ribs absent or extending onto basal quarter only. Stipe 0.5–1.5 cm long, 0.3–0.5 cm broad, flaring and merging with apothecium, white, becoming cream and tough when dried, glabrous to finely pubescent, ribs prominent, rounded, few anastomosis between ribs, basal mycelium white. Medullary excipulum 220–315 lm broad, of textura intricata, hyaline, composed of 3–4 lm broad hyphae. Ectal excipulum 60–115 lm broad, of textura angularis, hyaline, evenly blue in cotton blue, outermost cells 17–45 9 7–27 lm, clavate to subglobose. Stipitipellis 100–160 lm, hyaline, composed of textura angularis, terminal cells 14–59 9 8–21 lm, clavate. Asci 260–330 9 15–21 lm, aporhynchous base, 8-spored, uniseriate, subcylindrical to clavate. Paraphyses filiform, slightly exceeding the asci, with a yellow refractive content in Melzer’s reagent, 3–5 lm broad. Ascospores [80/4/4, in H2O] (17–)18–20(–21) 9 11–13 lm [Q = 1.36–1.77, Q = 1.59 ± 0.09)], subellipsoid, smooth-walled under the light microscope. All tissues J-. Asexual morph Undetermined. Known distribution: Scattered or gregarious on moss under Picea euphratica, P. retroflexa Mast. and P. purpurea Mast. Picea spp. forests. Known in Europe (Harmaja 1978) and Asia (Liu et al. 1985). Material examined: CHINA, Sichuan Province, Jiuzhaigou County, on forest ground with Picea euphratica Oliv., alt. 3200 m, 19 Jun 2014, Qi Zhao 2046 (HKAS 87726), Jiuzhaigou County, alt. 3300 m, 22 June 2014, Qi Zhao 2064 (HKAS 87744), same location, 23 June 2014, Qi Zhao T24352, T24387, T24470, T24477, respectively (HKAS 87693; HKAS 87694; HKAS 87698; HKAS 87700), Xinjiang Autonomous Region,Zhaosu County, alt. 2330 m, 9 July 2015, Qi Zhao 2380 (HKAS 90254). 157 Notes: Helvella oblongispora is characterized by deeply cupulate pileus, slightly serrate margin, brown, even to slightly undulate hymenium, white to pale brownish receptacle surface, and prominent, rounded ribs, white stipe without few crossed veins or pockets; aporhynchous asci, paraphyses apex 3–5 lm broad and subellipsoid, 18–20 9 11–13 lm ascospores. The species was re-examined by Landeros et al. (2012). Helvella oblongispora is very similar to H. leucomelaena in gross morphology and anatomical structure. However, H. leucomelaena has dark grey to blackish apothecia, deeply cupuliform, generally subsessile or with a very short stipe and less or more visible ribs and 20–23 9 11–13 lm ascospores (Dissing 1966; Abbott and Currah 1997). Pezizaceae Dumort. Pezizaceae, known as the cup-fungi, is recognized by the fleshy, soft, brittle, cupulate ascomata, with amyloid asci. This family formed a strongly supported monophyletic with Ascobolaceae as its sister group (Hansen and Pfister 2006). To date, GBIF lists 54 genera, approximately 1214 species (http://www.gbif.org/species/8399, 27 July 2016) and, of these, 31 genera, 230 species were accepted (Kirk et al. 2008). Peziza Dill. ex Fr. Peziza is the type genus of the family Pezizaceae, estimated to comprise 104 species (Kirk et al. 2008). Molecular phylogenetic studies have shown that the genus Peziza is not monophyletic, composed of at least 14 distinct lineages (Hansen and Pfister 2006) (Figs. 100, 101). Peziza fruticosa Lantieri, Medardi & Vizzini, sp. nov. Index Fungorum number: IF552367; Facesoffungi number: FoF02450, Figs. 102 and 103 Etymology: from Latin ‘‘fruticosa’’, clustered, for its way of fruiting. Holotype: AMB 17135. Saprobic on the sand of the high dunes. Sexual morph Apothecium cup shaped, sessile, irregularly rounded or compressed-lobed because of mutual contact; on average 15 mm diam. Hymenium smooth, often wavy, dull to dark brown, with reddish reflections, darker on the bottom. Receptacle surface smooth, concolorous with hymenium; margin whole (rarely cracked), even to lobed, wavy, curved. Flesh fragile, waxy, up to 1.5 mm thick, pale brown. Medullar excipulum up to 950 lm thick, 3-layered, upper layer of textura globulosa-angularis, cells 30–80 (100) lm diam.; intermediate layer of textura intricate; hyphae 4–6 lm diam., with presence of a few rounded cells, 10–15 lm diam.; lower layer identical to the upper 123 158 Fig. 109 Diaporthe garethjonesii (MFLU 13-0261, holotype). a Diseased leaves. b Conidiomata on the host surface. c Longitudinal section of conidiomata. d Conidiomata on host. e–g Conidiogenous cells with developing conidia. h–m Conidiogenous cells with 123 Fungal Diversity (2016) 80:1–270 developing conidia stained with lactophenol cotton blue. n Alpha conidia. o Beta conidia. p Germinating conidium. q, r Colonies on PDA, q from above, r from below. Scale bars c, = 200 lm, e– p = 10 lm Fungal Diversity (2016) 80:1–270 Fig. 110 Diaporthe siamensis (MFLUCC 12-0300, NTCL056-1). a Herbarium specimen. b Appearance of conidiomata on the host surface. c Vertical section of conidioma. d Peridium. e Ostiole. f, g Conidiogenous cells with developing conidia. h Alpha conidia. 159 i Beta conidium. j Germinating alpha conidium. k–l Culture on PDA, k top, l reverse. Scale bars b = 500 lm, c, d = 100 lm, e = 50 lm, f–j = 10 lm 123 160 Fungal Diversity (2016) 80:1–270 Fig. 111 Maximum parsimony (MP) majority rule consensus tree of Cytospora isolates based on a combined dataset of ITS, LSU, RPB2 and ACT sequence data. Values above the branches indicate maximum parsimony and maximum likelihood bootstrap C70 %, (MPBS/ MLBS). Values at the third positions, respectively, above or below the branches represent posterior probabilities (BI PP C 0.90) from Bayesian inference analysis. The tree is rooted with Phomopsis vaccinii (ATCC 18451). The strain numbers are mentioned after the species names. The species obtained in this study are in blue bold and ex-type strains are in black bold one. Ectal excipulum up to 250 lm thick, textura globulosa or globulosa-angularis, cells 15–20 lm diam. Subhymenium up to 100 lm thick, textura globulosa-angularis (cells 5–10 lm diam.), mixed with intricate hyphae. Asci 190–225 9 12–15 lm, cylindrical, amyloid, 8-spored, amyloidity visible only on the top, lower part manifestly destrinoid. Paraphyses clavate to irregularly swollen, several bent in the upper part, a few with oil drops, septate, simple or forked, with some moniliform cells, 2.5–4 lm in the lower part, up to 10 lm at the apex. Ascospores 15–17 9 7.5–9.5 lm, elliptical, distinctly and very delicately warted, ornamentation as very fine warts, punctiform or slightly elongated (0.4–0.6 lm), hyaline, 123 without oil drops, uniseriate in the ascus. All tissues hyaline to brownish, except the ectal excipulum that is pale reddish-brown. Asexual morph Undetermined. Habitat and known distribution: on the sand in dune zones and on high dunes, near degraded remnants of Ammophila arenaria and Eryngium maritimum; so far known only from Italy and Spain. Autumn-spring. Material examined: ITALY, Calambrone, Pisa, Leg. G. Medardi, 26 April 1996, AMB 17136, Rosolina Mare, Rovigo, Leg. A. Lantieri and G. Medardi, 7 December 2014, AMB 17135 (holotype). SPAIN, Playa de Xagò, Asturias, Leg. C. Lopez-Alvarez, 15 February 2006, K(M) 140524 (sub ammophila). Fungal Diversity (2016) 80:1–270 161 Fig. 112 Maximum parsimony (MP) majority rule consensus tree of Cytospora isolates based on ITS sequence data. Values above the branches indicate maximum parsimony and maximum likelihood support C 70 %, (MPBS/MLBS). Values at the third positions, respectively, above or below the branches represent posterior probabilities (BI PP C 0.90) from Bayesian inference analysis. The tree is rooted to Phomopsis vaccinii. New strains are in blue bold and ex-type strains are in black bold Notes: In the year 1996, on the dunes near Calambrone (Pisa, Italy), we collected one portion of a strange fungal structure, made up by a sort of hemisphaerical accumulation of small, brown apothecia, quite overlapping; due to the particularly rainy period and the advanced age, the specimen was not in good conditions to be photographed, but we preserved it however (AMB 17136) because of its interesting microscopic characters. In 2006 one Spanish colleague collected one identical formation on sand in Asturias, which revealed the same microscopic features, still referable to any known species; we placed this collection in herbarium under a provisional name [K(M) 140524], waiting for other more detailed studies. In December 2014 near Rosolina Mare (Rovigo, Italy) we newly found this fungus (AMB 17135). The microscopic study showed the same characteristics of the two previous collections, and the following molecular analyses confirmed it as a new species. According to current information, Peziza fruticosa seems to be a rare species, however its easily identifiable 123 162 Fungal Diversity (2016) 80:1–270 Fig. 113 Cytospora cotini (holotype). a Appearance of fruiting bodies in wood. b Fruiting bodies on substrate. c Close up of fruiting body. d Cross section of the conidioma. e Peridium. f Conidiophores with conidia. g Conidia. h Germinating spore. i Culture characters on MEA. Scale bars a = 2 mm, b = 1 mm, c = 400 lm, d = 300 lm, e = 20 lm, f, g = 10 lm, h = 40 lm by well-defined macro- and microscopic features, such as the clustered-overlapping apothecia, amyloid asci only at the apex, and the manifest destrinoidity. Phylogenetically, P. fruticosa is closely related to P. domiciliana, P. perdicina P. ampliata and P. ninguis (Fig. 100). However, P. domiciliana Cooke differs by growing gregarious or occasionally cespitose on cellars, damp walls, plaster, mortar, mushroomcaves, and smaller ascospores, 14.5–15.5 9 7–8 lm, with two oil drops (Seaver 1917; Le Gal 1941; Maas Geesteranus 1967; Rifai 1968; Hansen et al. 2002). Peziza perdicina (Velen.) Svrček has ascospores 15–16 9 6–7 lm, and occurs on dung (Svrček 1976; Donadini 1981; Häffner 1985; Cacialli et al. 1997; Doveri 2004; Medardi 2006). Peziza ampliata Pers. has ascospores 18–20.5 9 9.5–11.5 lm, smooth and grows on hardwood trunks, rich soil mixed with wood-chips, chalk (Svrček 1970; Dennis 1981; Hansen et al. 2002; Medardi 2006). Concerning P. ninguis Donadini & Trimbach, we had the possibility to examine the collection ref. JF908536, consisting of one specimen kept in MCVE (ref. 11883), and we noticed it fully matches the characters of P. varia (Hedw. : Fr.) Alb. & Schwein; the name under which its sequence was filed in GenBank is therefore incorrect. P. ninguis is one of the synonyms of P. nivalis (R. Heim & L. Rémy) M.M. Moser, that has divergent microscopic characters: ascospores remarkably larger and differently ornamented, 18–22.5 9 9–10 lm vs. 14–16 (17.5) 9 9–11 (12) lm for P. varia (Medardi et al. 2012), with a very feeble and inconstant punctuation instead of the typical superficial crimp, and growth in nival environment only in solstitial period, often immersed in water from melting snow fields. The fungi of MCVE 11883 were collected on 04/05/1996 in the municipality of Trasaghis (Udine, Italy), one town located only a few hundred meters above sea 123 Fungal Diversity (2016) 80:1–270 level and therefore not in the expected typical glacial environment, and moreover in an inappropriate period. The sample was previously identified as ‘‘P. ninguis var. fortoulii’’, but also in this case the characters are not coincident, because the ascospores of P. fortoulii Donadini & Neville are larger [16–18 (19) 9 10.5–12.5 lm] and smooth (our personal observations). Among the morphologically closest species, P. proteana (Boud.) Seaver f. sparassoides (Boud.) Korf shares similar habits with P. fruticosa, such as several apothecia often coalesced into a large, irregular compound cauliflower-like mass, but differs in smaller ascospores 10–13 9 5–7 lm with a strongly warty ornamentation, and by growing on 163 burnt soil (Seaver 1917, 1928; Durand 1919; Korf 1956, 1973; Dennis 1981; Donadini 1981; Geesink 1984; Hohmeyer 1986; Van Vooren 2003; Medardi 2006; Barseghyan and Wasser 2007). The sand-associated P. ammophila Durieu & Lév. shows semi-hypogean, funnel-shaped not coalesced apothecia, at first sphaerical and then opened and splitting into a stellate shape, long stalked, and ascospores 16–18 9 9.5–11 lm, elliptical and smooth (Donadini 1981; Hansen et al. 2002). Sordariomycetes For Sordariomycetes for follow Maharachchikumbura et al. (2016). Fig. 114 Phylogram generated from maximum parsimony analysis based on combined ITS, GADPH, CHS, ACT and TUB2 sequence data from species of dematium species complex. Maximum parsimony bootstrap support values greater than 50 % and Bayesian posterior probabilities greater than 0.70 are shown above the branches. The new isolate is in blue. The tree is rooted with Colletotrichum nigrum 123 164 Fungal Diversity (2016) 80:1–270 Fig. 115 Colletotrichum insertae (holotype). a Appearance of conidiomata on host. b Black acervuli with setae. c Setae. d Blunt rounded tip of setae. e Base of the setae. f–h Conidiogenous cells. i– l Conidia. Scale bars a, b = 200 lm, c = 50 lm, d = 5 lm, e = 150 lm, f = 20 lm g–l = 5 lm Coronophorales Nannf. Member of the order Coronophorales are wood inhabiting fungi and comprise the families Bertiaceae, Chaetosphaerellaceae, Coronophoraceae, Nitschkiaceae and Scortechiniaceae (Maharachchikumbura et al. 2015, 2016). The taxa in the order are characterized by mostly superficial ascomata, sometimes with an extensive hyphal subiculum or well-developed basal stroma, that often becomes cupulate or collapsed, and in some cases the ostiolar opening is either indistinct or lacking (Mugambi and Huhndorf 2010; Maharachchikumbura et al. 2016). characterized by immersed, erumpent or superficial ascomata, lack of ostiole, thin-walled asci with long stipe and large ascospore numbers. Maharachchikumbura et al. (2015, 2016) showed the family Coronophoraceae is monophyletic, however it was only represented by the type species C. gregaria. Coronophoraceae Höhn. The order and family were treated by Maharachchikumbura et al. (2015, 2016) and is followed here. Coronophora Fuckel The genus Coronophora was introduced by Fuckel (1864) and Mugambi and Huhndorf (2010) reported a single collection of Coronophora gregaria Fuckel from USA. It is 123 Coronophora myricoides H.X. Wu & K.D. Hyde, sp. nov. Index Fungorum number: IF552180; Facesoffungi number: FoF02451, Fig. 104 Etymology: Referring to the resemblance of the ascomata (under the stereomicroscope) to the berries of Myrica rubra (waxberry). Holotype: IFRD 9201. Saprobic on the surface of dead wood. Asexual morph Undetermined. Sexual morph Ascomata 373–395 lm high 9 282–340 lm diam. ( x = 383 9 308 lm, n = 10), perithecial, superficial or erumpent through bark of host, clustered in small to large groups, subglobose, surface tuberculate, dark brown to black, lacking ostioles. Fungal Diversity (2016) 80:1–270 165 Fig. 116 Phylogram generated from maximum likelihood analysis based on combined LSU, ITS sequence data from species of Glomerellales. Maximum likelihood bootstrap support values greater than 50 % and Bayesian posterior probabilities greater than 0.90 are shown in above and below and branches with maximum parsimony bootstrap support values greater than 50 % are in bold. The new isolates is in red and other ex-type strains are in bold. The tree is rooted with Microascus longirostris Peridium 27–135 lm (27–56 lm at the apex, 93–135 lm at the base), composed of five layers of dark brown-walled cells of textura globosa, inner layer of hyaline pseudoparenchymatic cells, slightly melanized on the outer surface. Paraphyses not seen. Asci 102–147 9 11–27 lm ( x = 119 9 22 lm, n = 15), numerous, polysporous, unitunicate, cylindrical to clavate, pedicel 11–38 9 4– 7 lm. Ascospores 6–8 9 1–2 lm ( x = 7 9 1.7 lm, n = 30), numerous, hyaline, 1-celled, cylindrical or ellipsoidal, narrowly rounded at both ends, smooth-walled. Material examined: CHINA, Yunnan Province, Jing Dong, on dead wood of unidentified plant, 13 September 2013, Wu Hai Xia (IFRD 9201, holotype). Notes: The genus Coronophora is typified by Coronophora gregaria and there are 28 epithets in Index Fungorum (2016). Kirk et al. (2008) estimated that there are five species in Coronophora from wood. There are two sequences for Coronophora gregaria in GenBank. In this paper, we introduce a new species of Coronophora; C. myricoides which differs from C. gregaria in the shape of the ascomata and also the shape of ascospores. The ascospores of Coronophora myricoides (6–8 9 1–2 lm) are smaller the type species (8–12 9 2–3 lm). We directly extracted DNA using ascomata and sequencing. We add the molecular data to better understand the generic and familial circumscriptions. 123 166 Fig. 117 Blastophorum aquaticum (DLU084, holotype). a–c Conidiophores and conidia. d Conidiogenous cell. e–k Conidia. l Germinating conidium. m Surface view of culture on MEA. n Reverse view 123 Fungal Diversity (2016) 80:1–270 of culture on MEA. Scale bars a = 60 lm, b = 50 lm, e, l = 20 lm, c = 15 lm, d = 10 lm, f–k = 5 lm Fungal Diversity (2016) 80:1–270 Diaporthales Nannf. Members of Diaporthales are pathogens, parasites, and endophytes of plants, human-animal pathogens, saprobes and soil inhabitants (Rossman et al. 2007). The order Diaporthales is characterized by perithecia with an elongate beak, often forming within stromatic tissues (Rossman et al. 2007). Asci generally deliquesce at the base when mature and have a characteristic refractive apical annulus. The order Diaporthales comprises 12 families: Cryphonectriaceae, Diaporthaceae, Gnomoniaceae, Harknessiaceae, Macrohilaceae, Melanconidaceae, Pseudoplagiostomataceae, Pseudovalsaceae, Schizoparmaceae, Stilbosporaceae, Sydowiellaceae and Valsaceae (Maharachchikumbura et al. 2016). Diaporthaceae Höhn. ex Wehm. Von Höhnel (1917) established the family Diaporthaceae and accommodated it in the order Diaporthales. The family has undergone various taxonomic revisions during the past century (Wehmeyer 1975; Barr 1978; Castlebury et al. 2002; Dai et al. 2014). Maharachchikumbura et al. (2015) accepted eleven genera in Diaporthaceae: Allantoporthe, Apioporthella, Clypeoporthella, Diaporthe, Diaporthella, Diaporthopsis, Leucodiaporthe, Mazzantia, Mazzantiella, Ophiodiaporthe and Pustulomyces. Diaporthe Nitschke Diaporthe has a worldwide distribution as endophytes, pathogens and saprobes (Udayanga et al. 2011). The asexual morph was previously known as Phomopsis. These genera were linked in Wijayawardene et al. (2014a) and Maharachchikumbura et al. (2015). Rossman et al. (2015) proposed to conserve Diaporthe over Phomopsis, to resolve nomenclatural problems. An update of the phylogeny of Diaporthales species with new species and records is presented herein (Fig. 105). Diaporthe aseana Dissanayake, Tangthirasunun & K.D. Hyde, sp. nov. Index Fungorum number: IF551402; Facesoffungi Number: FoF00925, Fig. 106 Etymology: In reference to the ASEAN, the ‘Association of Southeast Asian nations’ where the fungus was collected in Thailand, one of the member countries. Holotype: MFLU 13-0256. Saprobic on dead leaves. Sexual morph Undetermined. Asexual morph Conidiomata 140–200 lm diam. 9 220– 300 lm high ( x = 185 9 260 lm, n = 10), associated with necrotic leaf tissue; pycnidial, globose, unilocular, black, erumpent, ostiolate, walls comprising 5–6 layers of dark brown cells of textura angularis. Conidiophores 8–15 9 2–3 lm, hyaline, smooth, densely aggregated, cylindrical, straight to sinuous. Conidiogenous cells 167 6–10 9 2–3 lm, phialidic, cylindrical, terminal and lateral, slightly tapering towards the apex. Paraphyses not observed. Alpha conidia 6–9 9 2–3 lm, aseptate, hyaline, smooth, guttulate, fusoid to ellipsoid, tapering towards both ends, straight, apex subobtuse. Beta conidia not observed. Culture characteristics: Colonies covering 9 cm Petri dish after 2 weeks in the dark at 28 °C. On PDA surface with fluffy white aerial mycelium with patches of saffron, in reverse patches of luteous to olivaceous-grey. Material examined: THAILAND, Phayao, Jam Pa Thong Waterfall, on dead leaf, 12 March 2012, N. Tangthirasunun (MFLU 13-0256, holotype); ex-type living cultures MFLUCC 12-0299a, KUMCC 15-0112. Notes: Diaporthe aseana did not produce a sexual morph in culture or on the host. Multi-gene phylogenies reveal a close relationship of D. aseana to D. hongkongensis, D. lithocarpus, D. eucalyptorum, but support our new taxa as not being conspecific. Our phylogenetic analyses position D. aseana basal to the above species (Fig. 105). Based on a Blast search of NCBI’s GenBank nucleotide database, the closest matches for the ITS sequence of D. aseana are an undescribed endophytic Diaporthe sp. InaCC F-238 from Cinchona calisaya, Indonesia (AB899784; Identities = 544/549 (99 %), Gaps = 2/549 (0 %), and an endophytic Diaporthe sp. M23-2 from Abies beshanzuensis from Zhejiang Province, China (HM595506; Identities = 538/549 (98 %)). Diaporthe eres Nitschke, Pyrenomycetes Germanici 2: 245 (1870) Facesoffungi number: FoF02182, Fig. 107 Saprobic on dead cone of Picea excelsa found on land. Sexual morph Undetermined. Asexual morph Conidiomata 225–400 lm diam. 9 235–280 lm high ( x = 330 9 255 lm, n = 10), pycnidial, sub-globose to globose, unilocular, black, immersed to sub-immersed, ostiolate, peridium consisting of 3–5 layers of dark brown cells of textura angularis. Conidiophores 5–9 9 1–1.5 lm, hyaline, smooth, cylindrical, straight to sinuous. Conidiogenous cells 0.5–1 lm diam, phialidic, cylindrical, terminal and lateral, with slight taper towards apex. Paraphyses not observed. Alpha conidia 5–6 9 2–3 lm aseptate, hyaline, smooth, guttulate, fusoid to ellipsoid, tapering towards both ends, straight, apex subobtuse. Beta conidia not observed. Culture characteristics: Colonies white and pale brown on surface, reverse pale brown to black. Aerial mycelium white and brown, feathery, with concentric zonation, margin fimbriate, with visible conidiomata at maturity. Material examined: ITALY, on dead cone of Picea excelsa (Pinaceae), 12 April 2012, Erio Camporesi (MFLU 13-0334), living cultures MFLUCC 12-0351. Note: Diaporthe eres is an important plant pathogen (Gomes et al. 2013; Udayanga et al. 2014a, b; Dissanayake 123 168 123 Fungal Diversity (2016) 80:1–270 Fungal Diversity (2016) 80:1–270 169 et al. 2015) and has been reported on various woody hosts (from 121 host genera from 61 host families; https://nt.arsgrin.gov/fungaldatabases). This is the first record of D. eres on Picea excelsa. In our phylogenetic analysis of combined ITS, EF-1a, b-tubulin and CAL sequence data of Diaporthe species (Fig. 105), strain MFLUCC 12-0351 grouped together with ex-type strains of D. eres (ex-epitype culture AR5193). Diaporthe foeniculina Niessl, in von Thümen, Contr. Ad. Fl. Myc. Lusit. 2: 30. 1880. Facesoffungi number: FoF02183, Fig. 108 Saprobic on dead branch of Laburnum. Sexual morph Undetermined. Asexual morph Conidiomata 215–390 lm diam. 9 160–235 lm high ( x = 360 9 185 lm, n = 10), pycnidial, eustromatic, unilocular, semi-immersed, dark brown, scattered or aggregated, ostiolate, peridium consisting of brown thick-walled cells of textura angularis, conidial mass globose to conical and exuding in cirrhi, yellow to reddish brown. Conidiophores 20–28 9 2–3 ( x = 24 9 2 lm, n = 10) lm hyaline, subcylindrical or cylindrical, filiform, tapering towards the apex. Alpha conidia 6–9 9 2–3 lm ( x = 7 9 3 lm, n = 10), hyaline, oblong to ellipsoidal, apex bluntly rounded, base obtuse to Fig. 119 Ophiocordyceps hemisphaerica. a Overview of the stromata and the host (FURB 45181). b Fertile head (FLOR 59525, holotype). c Cross section showing the complete immersed perithecia. d Ascoma entirely isolated from the fertile head. e Conidia. f Asci. g Apical cap. h–i Asci with part-spores. Scale bars a = 2 cm, b–c = 1 mm, d = 1000 lm, e = 10 lm, h–i = 10 lm b Fig. 118 Phylogram generated from Bayesian analysis based on ITS sequence data of Ophiocordyceps. Metacordyceps taii is used as outgroup taxon. Maximum likelihood bootstrap values greater than 50 % and Bayesian posterior probabilities over 0.90 are indicated above and below the nodes (BBP/BP), respectively. The new species are indicated in blue, holotypes in bold 123 170 Fungal Diversity (2016) 80:1–270 sub-truncate, bi- to multi-guttulate. Beta conidia 26– 34 9 1–2 lm ( x = 28 9 1 lm, n = 10), hyaline, smooth, slightly curved. Culture characteristics: Colony is entirely white both on surface and reverse. Aerial mycelium cottony and feathery, colonies reaching 60 mm diam. after 5 days in 28 °C. Fig. 120 Ophiocordyceps lacrimoidis. a Holotype, overview of the stromata and the host attached by mycelium on a twig. b Fertile region. c Fertile head in a cross section showing the complete immersed perithecia. d Vertical section showing the superficial perithecia. e Asci. f Layer of conidiogenous cells with a conidium attached. g Conidia. Scale bars a = 5 mm, b–d = 1 mm, e = 20 lm, f = 20 lm, g = 25 lm 123 Fungal Diversity (2016) 80:1–270 Material examined: ITALY, on dead branch of Laburnum (Fabaceae), 21 August 2012, Erio Camporesi (MFLU 13-0320), living cultures MFLUCC 12-0668. Note: Diaporthe foeniculina has been reported on various woody hosts (from 20 host genera from 16 host families; https://nt.ars-grin.gov/fungaldatabases). This is the first record of D. foeniculina on Laburnum sp. In our phylogenetic analysis of combined ITS, EF-1a, b-tubulin and CAL sequence data of Diaporthe species (Fig. 105), strain MFLUCC 12-0668 grouped together with ex-type strains of D. foeniculina with very high support (ex-epitype culture CBS 111553). Diaporthe garethjonesii Dissanayake, Tangthirasunun & K.D. Hyde, sp. nov. Index Fungorum number: IF551403; Facesoffungi Number: FoF00926, Fig. 109 Etymology: In reference to the significant contribution of E.B. Professor Gareth Jones made to Thai mycology. Holotype: MFLU 13-0261. Saprobic on dead leaves. Sexual morph Undetermined. Asexual morph Conidiomata 85–125 lm diam. 9 80– 100 lm high ( x = 115 9 85 lm, n = 10), associated with necrotic leaf tissue; pycnidial, globose, unilocular, black, immersed to sub-immersed, ostiolate, walls consisting of 5–6 layers of dark brown cells of textura angularis. Conidiophores 5–12 9 1–1.5 lm, hyaline, smooth, cylindrical, straight to sinuous. Conidiogenous cells 0.5–1 lm diam., phialidic, cylindrical, terminal and lateral, with slight taper towards apex. Paraphyses not observed. Alpha conidia 5–6 9 2–3 lm, aseptate, hyaline, smooth, guttulate, fusoid to ellipsoid, tapering towards both ends, straight, apex subobtuse. Beta conidia 40–50 9 3–4 lm hyaline, smooth, less common than alpha conidia, straight, curved or hamate. Culture characteristics: Colonies with sparse aerial mycelium covering the dish after 2 weeks in the dark at 28 °C. On PDA buff, honey to isabelline, reverse smokegrey. Material examined: THAILAND, Kanjanaburi, on dead leaf, 5 May 2012, Jayarama Bhat (MFLU 13-0261, holotype), ex-type living cultures MFLUCC 12-0542a, KUMCC15-0117. Notes: Diaporthe garethjonesii forms a well-supported clade in our phylogenetic analysis with high bootstrap and Bayesian values (Fig. 105). The BLAST comparison of the ITS sequence of D. garethjonesii showed a 99 % match to a fungal endophyte from Hong Kong (DQ485955) (Identities = 543/548 (99 %), Gaps = 4/548 (0 %)), a 98 % match to the ITS sequence of USA isolate NY8658c (HQ108026) (Identities 537/550 (98 %), Gaps 4/550 (0 %)). The distinct clade that D. garethjonesii forms in the phylogenetic analysis, represents a separate species. 171 Diaporthe siamensis Udayanga et al., Cryptogamie Mycologie 33: 298 (2012) Facesoffungi number: FoF02398, Fig. 110 Saprobic on dead leaves of woody plants. Sexual morph Undetermined. Asexual morph Conidiomata pycnidial, 185–200 lm high, 150–200 lm diam. ( x = 190 9 170 lm, n = 10), ampulliform, scattered, immersed, ostiolate, with elongate black neck. Pycnidial wall, comprising 2–3 layers, with heavily pigmented outer layer, thick-walled, comprising blackish to dark brown cells of textura angularis, with lighter cells towards the inside, with inner layer composed of 1–2 layers, hyaline, thin-walled cells of textura angularis. Conidiophores reduced to conidiogenous cells. Conidiogenous cells enteroblastic with percurrent annellations, integrated, solitary, hyaline, smooth-walled and formed from the inner layer of pycnidium wall. Paraphyses extending above conidiophores and hyaline, 20–35 lm long. Alpha conidia 3– 5 9 2–3 lm ( x = 3.5 9 2.4 lm, n = 30), hyaline, smoothwalled, mono- or bi-guttulate. Beta conidia15–20 9 1.5– 2 lm ( x = 18.5 9 1.8 lm, n = 30), aseptate, hyaline, hamate or curved, apex acutely rounded. Culture characteristics: Colonies on PDA 30 mm diam. after 4 weeks at 25 °C, cream to white mycelium, cottony and lobate at the margins, white at the center; reverse yellowish. Material examined: THAILAND, Chiang Rai Province, Thasud, Muang District, Mae Fah Luang University, on dead leaves of Castanopsis sp. (Fagaceae), 21 March 2012, N. Tangthirasunun (MFLU 13-0257, NTCL056-1), living culture, MFLUCC 12-0300. Notes: Diaporthe siamensis was introduced by Udayanga et al. (2012). This species was described on leaves of Dasymaschalon sp. (Annonaceae) from Chiang Rai, Thailand. Since then this species has not been reported from any locality worldwide. Here we have collected D. siamensis for the first time on Castanopsis sp. (Fagaceae) from a close locality in Thailand. The morphological characters are similar to the type specimen. Based on the phylogenetic analysis of combined ITS, EF1-a, CAL sequence data, shows high support to its ex-type strain MFLUCC 10-0573a, clusters with high support with D. siamensis MFLUCC 12-0300 (Fig. 105). According to Udayanga et al. (2012) this species was found in different habitats of Dasymaschalon sp. whereas, in this study we isolated it as a saprobic fungus. Identification of D. siamensis, within the same locality on two different plant groups, reveals the potential of this species to occur on multiple hosts in different habitats. Valsaceae Tul. & C. Tul. The family Valsaceae was introduced by Tulasne and Tulasne (1861) and placed in Diaporthales by Barr (1978). Most of Valsaceae species are plant pathogens causing canker and dieback disease, with damage to several 123 Kobayasi and Shimizu (1982) 6–9 9 1 – 5–6 diam. 620–700 9 200–250 2.5–3.5 9 0.7–1.2 Beneath a branch O. discoideicapitata (Japan) 650–700 9 200–250 4–5 9 1 On twigs (Brazil) O. lacrimoidis economic crops worldwide (Adams et al. 2005; Fan et al. 2014, 2015a, b; Ariyawansa et al. 2015a, Li et al. 2016, Maharachchikumbura et al. 2015, 2016). Currently this family comprises 13 genera: Amphicytostroma, Chadefaudiomyces, Cryptascoma, Cytospora, Ditopellina, Durispora, Harpostroma, Hypospilina, Kapooria, Leptosillia, Maculatipalma, Pachytrype and Paravalsa (Maharachchikumbura et al. 2015, 2016). Valsaceae was restricted to Cytospora as asexual morph and four sexual morph genera viz. Valsa, Leucostoma, Valsella, and Valseutypella (Fries 1838; Saccardo 1884; Gvritishvili 1982; Spielman 1985; Adams et al. 2002, 2005; Castlebury et al. 2002; Bulgakov 2010; Yang et al. 2015; Li et al. 2016; Maharachchikumbura et al. 2015, 2016). Therefore, Adams et al. (2005) synonymized all sexual genera under Valsa as a subgenus or species without additional infrageneric rank, according to the International Code of Nomenclature for Algae, Fungi, and Plants (ICN) in 2011 for placement on the list of protected fungi (Adams et al. 2005; Fotouhifar et al. 2010, Fan et al. 2015a; Wingfield et al. 2012; Crous et al. 2015a; McNeill et al. 2012; Rossman et al. 2015; Ariyawansa et al. 2015a, b, c; Li et al. 2016; Maharachchikumbura et al. 2015, 2016). Cytospora species have been reported as the asexual morph of most taxa in Valsaceae, and they are characterized by single or labyrinthine locules, filamentous conidiophores and allantoid, hyaline conidia. In moist conditions, conidia emerge from the fruiting bodies as yellow masses, which later become orange to red (Spielman 1983, 1985; Adams et al. 2005, 2006; Li et al. 2016; Maharachchikumbura et al. 2015, 2016). There are 579 epithets for Cytospora in Index Fungorum (2016). Ex-type sequence data are available for only a few species and it is, thus, difficult to identify isolates to the species level (Liu et al. 2015a; Ariyawansa et al. 2015a, b, c; Li et al. 2016; Maharachchikumbura et al. 2015, 2016). Further studies and recommended on Cytospora to clarify cryptic species with updated gene trees (Adams et al. 2002; Fotouhifar et al. 2010; Hyde et al. 2010, 2014; Fan et al. 2015a, b; Liu et al. 2015a; Ariyawansa et al. 2015a, b, c; Yang et al. 2015; Li et al. 2016). – 4–5 9 3–5 8–14 9 2 350–450 9 5 – 590–700 9 2 750–800 9 270–280 (Japan) O. dipterigena On twigs 3–6 9 0.3–0.5 – – – – 5–10 9 1 On twigs (Sri Lanka) O. dipterigena This study Kobayasi and Shimizu (1978) 4.5–9 9 1.5–2.5 – 10 9 1.5 – – 500–640 9 5–6 780–860 9 220–290 12–20 9 0.8–1 On twigs (Brazil) Ophiocordyceps hemisphaerica – Berkeley and Broome (1875) This study 6.2–8.3 9 2.5–3.5 – 11–14 9 2 – 550–700 9 5 850–920 9 230–300 9–13 9 0.5 Under the leaf Cordyceps muscicola 123 (Brazil) Conidia (lm) Part-spores (lm) Ascospores (lm) Asci (lm) Ascomata (lm) Stromata (mm) Host behavior Species Table 1 Synopsis of Ophiocordyceps species discussed in the paper Möller (1901) Fungal Diversity (2016) 80:1–270 Reference 172 Cytospora Ehrenb. Cytospora was introduced by Ehrenberg (1818), and it contains species that are one of the most important pathogens, causing canker disease on branches leading to large areas of dieback on a wide range of plants (Adams et al. 2005, 2006). The genus Cytospora has sexual morphs in Valsa, Leucostoma, Valsella, and Valseutypella (Adams et al. 2005). All sexual genera were synonymized with Valsa either as a subgenus or species. Cytospora (1818) is an older name than Valsa (1849) and the Cytospora morph is more common in nature. Therefore, Valsa species are treated as synonyms of Cytospora. Cytospora species Fungal Diversity (2016) 80:1–270 173 Fig. 121 Maximum Parsimony tree based on an analysis of combined ITS and b-tub sequence data. Bootstrap values greater than 50 % are indicated at the nodes. Type strains are shown in bold and the new taxon is in violet Fig. 122 Purpureocillium sodanum (holotype). a–c Colonies on MEA, CMA and CYA. d, e Subglobose conidia. f Acremonium-like conidiophores. g Paecilomyces-like conidiophores. h Cylindrical conidia. Scale bars d, g, h = 10 lm, e, f = 5 lm 123 174 Fungal Diversity (2016) 80:1–270 Fig. 123 Phylogram generated from maximum likelihood analysis based on combined LSU, ITS and RPB2 sequence data for taxa of Hypocreales. Maximum likelihood bootstrap support values greater than 50 % are shown near the nodes. The new isolates are in blue and ex-type sequences in bold. The tree is rooted with Gelasinospora tetrasperma and Neurospora crassa identification has generally been established according to host affiliation, while morphological descriptions generally lacked detail. However, a single species of Cytospora often occurs on taxonomically unrelated host plants (Adams et al. 2005; Wang et al. 2011, Fan et al. 2015a, b; Ariyawansa et al. 2015a, b, c; Liu et al. 2015a; 123 Fungal Diversity (2016) 80:1–270 175 Fig. 124 Alfaria spartii (holotype). a, b Ascomata on host substrate. c Cross section of ascoma. d Ostiole. e Peridium. f, g Asci. h– k Ascospores. l Colonies on MEA. Scale bars a, b = 500 lm, c = 100 lm, d = 50 lm, e, f, g = 20 lm, h–k = 10 lm 123 176 Maharachchikumbura et al. 2015, 2016). There are 579 epithets for Cytospora in Index Fungorum (2016) with an estimated 110 species in Kirk et al. (2008). In this study with introduce two new species with support from molecular data (Figs. 111, 112). Cytospora cotini Norphanphoun, Bulgakov & K.D. Hyde, sp. nov. Index Fungorum number: IF552231; Facesoffungi Number: FoF02365, Fig. 113 Etymology: from generic name of host plant, Cotinus coggygria. Holotype: MFLU 14-0783. Necrotrophic on dying branches of Cotinus coggygria Scop. Sexual morph Undetermined. Asexual morph Conidiomata 800–1000 lm diam. pycnidial, solitary, immersed in host tissue, multi-locule, dark brown, ostiolate. Ostiole 250–350 lm diam. at the same level as the disc surface. Peridium comprising a few to several layers of cells of textura angularis, with inner most layer thin, hyaline, outer layer brown to dark brown. Conidiophores reduced to conidiogenous cells. Conidiogenous cells enteroblastic, phialidic, formed from the innermost layer of pycnidial wall, hyaline, smooth. Conidia (4.9–)5.6– 6.5 9 0.8–1.4(–1.7) lm ( x = 5.9 9 1.2 lm, n = 30), unicellular, allantoid to subcylindrical, hyaline, smoothwalled. Culture characteristics: Colonies on MEA, reaching 1.7 cm diam. after 7 days at 25 °C, producing dense mycelium, lobate circular, with white rough margin, after 5 days, flat or effuse on the surface, without aerial mycelium. Material examined: RUSSIA, Rostov region, Shakhty city, near Grushevsky pond, shelterbelt artificial forest, on dead branches of Cotinus coggygria Scop. (Anacardiaceae), 18 May 2014, Timur S. Bulgakov (MFLU 14-0783, holotype; KUM, isotype); ex-type living cultures, MFLUCC 14-1050, KUMCC. Notes: Cytospora cotini is a weak pathogen on Cotinus coggygria Scop., and is often associated with Pseudocamarosporium cotinae Norphanphoun et al. The present study using morphology and phylogenetic analyses, places Cytospora cotini in Valsaceae. The new species has immersed, multi-locular conidiomata, with a single ostiole and shares common walls with the host tissue. Phylogenetic analyses, using ITS sequence data (Fig. 112), indicate that C. cotini can be distinguished from other species within the genus Cytospora. The analyses based on combined ITS, LSU, RPB2 and ACT sequence data also demonstrate that C. cotini separates from other sequenced species, and is close to Cytospora tanaitica Norphanphoun et al. (Figs. 111, 112). However, C. tanaitica differs in 123 Fungal Diversity (2016) 80:1–270 having a single locule with smaller conidia than our species. Glomerellales Chadef. ex Réblová et al. Order ‘‘Glomerellales’’ was proposed by Chadefaud (1960), without a Latin diagnosis which made it invalid. Glomerellales was validly published by Réblová et al. (2011) and comprises of four families Australiascaceae, Glomerellaceae, Plectosphaerellaceae and Reticulascaceae (Maharachchikumbura et al. 2016). Glomerellaceae Locq. ex Seifert & W. Gams The family Glomerellaceae was invalidly published by Locquin (1984) and was validated in Zhang et al. (2006). This family was accepted as one of the three families of Glomerellales in Réblová et al. (2011). Glomerellaceae is a monotypic family, mainly comprised pathogens, and characterized by the Colletotrichum asexual morph and the Glomerella sexual morph, which was synonymized under Colletotrichum (Hyde et al. 2014; Maharachchikumbura et al. 2015, 2016). Colletotrichum Corda This genus was introduced by Corda (1831), for C. lineola Corda (Damm et al. 2009). Colletotrichum comprises mainly pathogens, as well as endophytes and saprobes (Cannon et al. 2012; Hyde et al. 2014). Kirk et al. (2001, 2008) and Réblová et al. (2011) placed Colletotrichum in the family Glomerellaceae and Maharachchikumbura et al. (2015) further confirmed the placement of this genus. In the latter study the use of the name Colletotrichum over its sexual name Glomerella was followed. Colletotrichum insertae Jayawardena, Bulgakov & K.D. Hyde, sp. nov. Index Fungorum number: IF552260; Facesoffungi number: FoF02399, Fig. 115 Etymology: Based on the host species. Hoolotype: MFLU 15-1895. Saprobic on dying twigs and leaf stalks of Parthenocissus inserta (A. Kern.) Fritsch. Sexual morph Undetermined. Asexual morph Conidiomata 250–620 lm ( x = 420 lm, n = 10) diam., black, acervulus, oval, solitory, gregarious. Setae straight or ±bent, abundant, dark brown, becoming paler towards the apex, opaque, smoothwalled, septate, 1–5-septate, 55–150 lm long, base cylindrical, 6.7–8.9 lm diam., apex rounded, Conidiophores simple, to 20 lm long, hyaline to pale brown, smoothwalled. Conidiogenous cells 11–24 9 2.8–4.9 lm ( x = 8.5 9 2.5 lm, n = 20), hyaline, smooth-walled, cyllindrical to slighty inflated, opening 1–2 lm wide, collarette or periclinal thickening not observed. Conidia 17.3– Fungal Diversity (2016) 80:1–270 177 Fig. 125 Maximum likelihood tree based on an analysis of combined ITS and b-tub sequence data. Bootstrap values greater than 50 % are indicated at the nodes. New taxa are in violet, and other ex-type strains are in bold 23.4 9 2.6–5.2 lm ( x = 19.3 9 3.8 lm, n = 40), L/W ratio 5, hyaline, smooth or verruculose, aseptate, curved, gradually tapering towards the round to slightly acute apex and truncate base, guttulate. Appressoria not observed. Material examined: RUSSIA, Rostov Region, Rostovon-Don City, Botanical garden of Southern Federal University, High Park, dying twigs and leafstalks, on Parthenocissus inserta (L.) Planch. (Vitaceae), 15 April 2015, T.S. Bulgakov (T191), (MFLU 15-1895, holotype). Notes: No culture is available for this species; hence direct DNA extraction from conidiomata was conducted. The Colletotrichum dematium species complex is mainly characterized by curved conidia (Damm et al. 2009). Multigene analyses reveal a close association between C. insertae and C. dematium with high support (75 % BT/ 1.0 PP, Fig. 114). This species differs from C. dematium in having abundant setae per acervulus, which becomes hyaline towards the rounded apex and 1–5 septa, as well as smaller conidia (C. dematium conida L/W = 6). This species differs from C. lineola in having shorter setae with 1–5 septa which can be observed along the seta, conidiogenous cells not having a distinct collarette, as well as having smaller conidia (C. lineola conidia L/W = 6.6) (Fig. 115). Reticulascaceae Réblová & W. Gams The family Reticulascaceae was introduced to accommodate two holomorphic genera Reticulascus and Porosphaerellopsis Samuels & E. Müll., supported by analysis of combined ITS, LSU, SSU and RPB2 sequence data. Even though the characters and ontogeny of these genera differ, the centrum and interthecial tissues are quite similar (Réblová et al. 2011). Presently, three genera are accepted in Reticulascaceae (Maharachchikumbura et al. 2015, 2016). In this study, we place the genus Blastophorum in family Reticulascaceae based on phylogenetic analysis (Fig. 116) and morphological characters. Blastophorum Matsush Matsushima (1971) introduced the genus Blastophorum with B. truncatum as the type species. Index fungorum (2016) lists three additional species in this genus, namely B. fusarioides K. Matsush. & Matsush., B. pini Minter & Hol.-Jech., and B. uniseptatum Matsush. There is no sequence data in GenBank, and fresh collections are needed to provide a natural classification of the genus. Blastophorum aquaticum Z.L. Luo, Bhat & K.D. Hyde, sp. nov. Index Fungorum number: IF552241; Facesoffungi number: FoF02218, Fig. 117 Etymology: In reference to the aquatic habitat of this fungus. Holotype: DLU 084. Saprobic on submerged decaying wood. Sexual morph Undetermined. Asexual morph Colonies on the substrate superficial, effuse, velvety, brownish-grey to greyish-brown. Conidiophores macronematous, mononematous, unbranched, percurrently proliferating, smooth, septate, dark brown, 190– 250 lm ( x = 220 lm, SD = 26.5 lm, n = 10) long, 6– 8 lm ( x = 7 lm, SD = 1 lm, n = 10) wide. Conidiogenous cells terminal, integrated, initially enteroblastic, subsequently polyblastic and sympodial, subhyaline at the base and hyaline above, with inconspicuous flattened denticles at conidiogenous loci. Conidia 1–3-septate, elongated, cuneiform, rounded at the tip, narrowed towards the flattened base, smooth, slimy, 19–24 lm ( x = 21.5 lm, SD = 2.5 lm, n = 20) long, 6.5–7.5 lm ( x = 7 lm, SD = 0.5 lm, n = 20) wide. Conidial secession schizolytic. 123 178 Fungal Diversity (2016) 80:1–270 Fig. 126 Emericellopsis persica (holotype). a, b Colonies on CYA and MEA. c, d Immature and mature ascomata. e Ascospores. f Asci. g Acremonium-like conidiophores bearing hyaline conidia. h–j Chlamydospores. Scale bars c–j = 10 lm Culture characteristics: Colonies on MEA 70 mm diam. after 6 weeks at room temperature, dark brown at the margins, cream to white at the center; reverse yellowish to dark brown and orangish at the center, medium dense, circular, umbonate. 123 Material examined: CHINA, Yunnan Province, Dali, Cangshan Mountain, Heilong stream, saprobic on submerged decaying wood, March 2014, X.Y Liu, S-084 (DLU084, holotype), ex-type living culture, MFLUCC 15-0264. Fungal Diversity (2016) 80:1–270 179 Fig. 127 Phylogram generated from Bayesian analysis based on LSU sequence data from species of Meliolaceae. Maximum parsimony/likelihood bootstrap support values greater than 50 % and Bayesian posterior probabilities greater than 0.9 are shown in the first and second set, respectively. Ex-type strains are in bold and the new sequence are in blue bold Notes: Blastophorum resembles Kylindria in having terminal, integrated, initially enteroblastic, subsequently polyblastic and sympodial conidiogenous cells, but Blastophorum differs in having cuneiform and larger conidia. The phylogeny analysis (Fig. 116) also showed Blastophorum separates from Kylindria. Blastophorum aquaticum differs from B. pini in having elongated, cuneiform and wider conidia (6.5–7.5 lm vs. 1.5–2.5 lm) (Minter and Holuboá-Jechová 1981). It differs from B. truncatum in having longer conidiophores (190–250 lm vs. 18.3–132.6 lm), elongated, cuneiform, 1–3-septate and larger conidia (19–24 9 6.5–7.5 lm vs. 8.7–17 9 2.6– 4.3 lm) (Chen and Tezan 2008). Nectriaceae, Niessliaceae, Ophiocordycipitaceae, Stachybotriaceae and Tilachlidiaceae. Maharachchikumbura et al. (2016) Hypocreales Lindau Hypocreales is an order within the class Sordariomycetes, represented by Bionectriaceae, Clavicipitaceae, Cordycipitaceae, Flammocladiaceae, Hypocreaceae, Ophiocordycipitaceae G.H. Sung et al. Ophiocordycipitaceae was proposed in Sung et al. (2007), based mainly on phylogenetic analyses. According to most recent studies on the family, it contains six genera, Drechmeria, Harposporium, Ophiocordyceps, Polycephalomyces, Purpureocillium and Tolypocladium (Quandt et al. 2014). Ophiocordyceps was first described to accommodate species possessing ascospores that usually do not break into part-spores at maturity, asci with thin apical caps (Petch 1931a, 1932), and often producing a dark pigmented, tough to pliant stromata, commonly with perithecial apices (Sung et al. 2007). This genus is associated with several asexual morphologies, Sorosporella, Hirsutella, Hymenostilbe, Stilbella, Syngliocladium, and 123 180 Fig. 128 Meliola citri-maximae (MFLU14-0288, holotype). a Host leaves. b Colony on surface of leaf. c Ascoma on host substrate. d Hyphae with hyphopodia and phialides. e Hyphae with hyphopodia. f Hyphae with phialides. g Outer layer of peridium. h Inner layer of 123 Fungal Diversity (2016) 80:1–270 peridium. i Hamathecium. j Hyphal setae. k–q Asci from young state to mature state. r–t Ascospore from young state to mature state. Scale bars c, d = 200 lm, j = 100 lm, e–i, k–t = 20 lm Fungal Diversity (2016) 80:1–270 181 Fig. 129 Meliola pseudosasae (MFU 16-2136, reference specimen). a Herbarium specimen. b Appearance of ascomata on host leaf. c Ascoma. d Setae. e Vertical section of ascoma. f Superficial hyphae. g, h Ascospores. Scale bars d, e = 50 lm, f = 10 lm, g, h = 20 lm Paraisaria (Quandt et al. 2014). Hymenostilbe was proposed by Petch (1931b) and contains species that produce conidia from multiple denticles on conidiogenous cells forming a palisade-like layer along the surface of synnemata (Mains 1950). There is evidence to restrict the use of Hymenostilbe to the ‘‘O. sphecocephala clade’’ within the genus Ophiocordyceps (Sung et al. 2007). The phylogenetic tree, based on ITS, composed by species of this clade, including two new species, is presented in Fig. 118. Ophiocordyceps hemisphaerica Mafalda-Freire, Reck & Drechsler-Santos, sp. nov. Index Fungorum number: IF552122; Faces of fungi number: FoF02193, Fig. 119 Etymology: Referring to the hemisphaeric form of the fertile head of the stroma. Holotype: FLOR 59525 Specimens found on twigs of living plants, parasitic in true flies of Muscidae. Sexual morph capitated. Stromata 12–20 9 0.8–1 mm, rarely branched, stipitate, usually 2, up to 4, arising from the host thorax between the wings, tough, with morphology unaffected when dried. Stipe 11– 19 mm long, 0.8–1 mm wide, cylindrical, with a fertile apex, flexible when fresh, hard and tough when dried, 123 182 Fungal Diversity (2016) 80:1–270 Fig. 130 Consensus tree resulting from a Bayesian analysis of the ITS sequence alignment of Arthrinium species. RAxML bootstrap support values (MLB above 50 %) and Bayesian posterior probabilities (PP above 50 %) are given at the nodes (MLB/PP). The newly introduced sequences are in blue bold. The scale bar represents the expected number of changes per site. The tree is rooted to Xylaria hypoxylon smooth, brown to greyish-brown, epidermal layer compact, medullar region white to cream, not compacted. Fertile head 1–1.2 mm long, 2–4 mm diam., hemisphaerical, truncate, applanate at the base, pale to dark yellowish, ostioles inconspicuous on the upper surface, smooth-like. Ascomata obpyriform, slightly curved, 780–860 9 220– 290 lm ( x = 820 9 255 lm, n = 40), completely immersed, yellow, thick-walled, perithecia easily detachable from the fertile region. Asci narrow cylindrical, 500– 640 9 5–6 lm ( x = 570 9 5.5 lm, n = 40), apex thickened, 8-spored, hyaline. Apical cap 5–6 lm ( x = 5.5 lm, n = 40) diam. Ascospores filiform, smooth, almost as long as asci, hyaline, more than 52 septa, easily breaking into part-spores. Part-spores cylindrical to unusually fusoid, 7– 10 9 1–1.5 lm ( x = 8.5 9 1.25 lm, n = 40), hyaline, smooth. Asexual morph Hymenostilbe-like. Synnemata cylindrical, 6–12 9 0.5–1 mm, simple or branched, 1 to 3, smooth, brown to greyish-brown, arising from the thorax and abdomen of the host, unusually arising from aborted stromata. Conidiogenous cells phialidic, clavate, surface slightly rugose, hyaline, compactly arranged. Conidia obovoid, 6.2–8.3 9 2.5–3.5 lm ( x = 7.25 9 3 lm, n = 40), hyaline, slightly verrucous, with persistent appendix. 123 Fungal Diversity (2016) 80:1–270 183 Fig. 131 Arthrinium paraphaeospermum (holotype). a Colony on MEA. b Sporodochium on MEA. c–f Conidiogenous cells giving rise to conidia. h Conidia in side view. g Conidia and sterile cells. i Conidia in surface view. Scale bars a, b = 100 lm, c–i = 10 lm Material examined: BRAZIL. Santa Catarina, Ituporanga, Rio do Norte, 27°240 0000 S, 49°360 0000 W, on dead flies of Muscidae, 19 October 2014, Bittencourt FB 246 (FURB 45181); Florianópolis, 27°350 3300 S, 48°280 4200 W, 2010, Mafalda-Freire FMF 33 (FLOR 59524); Joaçaba, Parque Natural Municipal Rio do Peixe, 278100 2200 S, 518300 2300 W, 10 March 2010, Mafalda-Freire FMF 104 (FLOR 59525, holotype); 24 January 2014, Mafalda-Freire FMF 331 (FLOR 59526); Mafalda-Freire FMF 332 (FLOR 59527); Mafalda-Freire FMF 333 (FLOR 59528); 27 September 2014, Mafalda-Freire FMF 295 (FLOR 59529); Mafalda-Freire FMF 305 (FLOR 59530); 27 September 2014, Mafalda-Freire FMF 296 (FLOR 59531); MafaldaFreire FMF 297 (FLOR 59532); Mafalda-Freire FMF 298 (FLOR 59533); Mafalda-Freire FMF 309 (FLOR 59534); Mafalda-Freire FMF 310 (FLOR 59535); Mafalda-Freire FMF 311 (FLOR 59536); Mafalda-Freire FMF 312 (FLOR 59537); Mafalda-Freire FMF 313 (FLOR 59538); MafaldaFreire FMF 314 (FLOR 59539); Mafalda-Freire FMF 315 (FLOR 59540); Mafalda-Freire FMF 316 (FLOR 59541); Mafalda-Freire FMF 317 (FLOR 59542); Mafalda-Freire FMF 318 (FLOR 59543); Mafalda-Freire FMF 319 (FLOR 59544); Mafalda-Freire FMF 320 (FLOR 59545); Mafalda- Freire FMF 321 (FLOR 59546); Mafalda-Freire FMF 322 (FLOR 59547); Mafalda-Freire FMF 323 (FLOR 59548); Mafalda-Freire FMF 324 (FLOR 59549); 28 September 2014, Mafalda-Freire FMF 328 (FLOR 59553); Águas Mornas, Sı́tio Portal, 27°410 4700 S, 48°490 2900 W, 26 October 2013 Mafalda-Freire FMF 141 (FLOR 59550); Blumenau, Parque das Nascentes, 26°570 4100 S, 49°040 1100 W, 22 February 2012, Drechsler-Santos DS 785 (FLOR 59551). Additional material examined (as Ophiocordyceps dipterigena): JAPAN, Ibaraki: Gozenyama, 29 July 2001, F. Ihara 1021 (personal collection of F. Ihara); 26 July 2002, F. Ihara 02024 (personal collection of F. Ihara); F. Ihara 2025 (personal collection of F. Ihara); 7 August 2002, F. Ihara 04063 (personal collection of F. Ihara); 23 July 2007, F. Ihara 7044 (personal collection of F. Ihara); F. Ihara 07045 (personal collection of F. Ihara); Kyushu, July 1950, (MICH 274571); USA, North Carolina, Cranberry, August 1887, R. Thaxter, Reliquiae Farlowianae 612 (FH 5086); R. Thaxter, Reliquiae Farlowianae 612 (FH 5077); R. Thaxter, Reliquiae Farlowianae 612 (MICH 274593). Notes: Ophiocordyceps hemisphaerica is characterized by the tough stipitate stromata, with hemisphaerical and pale to dark yellowish fertile-head, with applanate base. 123 184 Fungal Diversity (2016) 80:1–270 Fig. 132 Phylogenetic tree of Neotruncatella endophytica EMLAS5-1 and EML-AS5-2 and related species based on maximum likelihood analysis of ITS rDNA sequences. Sequence of Pestalotiopsis malayana was used as outgroup. Numbers at the nodes indicate the bootstrap values ([50 %) from 1000 replications. The bar indicates the number of substitutions per position. New taxa are in blue The tough stromata are resistant and the morphology is unaffected even when dried. The primordium stromata has conidiogenous cells and conidia during the development. Additionally, the species parasitizes true big flies of Muscidae, which are not covered by mycelium. All parasitized flies were found above branches of living trees and always in advanced stage of decomposition. ITS based phylogenies group all O. hemisphaerica isolates in a strongly supported monophyletic clade and sharing a sister relationship to O. dipterigena species (Fig. 118). Ophiocordyceps hemisphaerica is morphologically similar to O. dipterigena (Berk. & Broome) G.H. Sung et al. However, it can be differentiated by its larger stromata (12–20 9 0.8–1 mm vs. 5–10 9 1 mm) and hemisphaerical fertile region, which are not observed in O. dipterigena (Petch 1932; Kobayasi and Shimizu 1978, Luangsa-ard et al. 2008). Additionally, O. dipterigena was described originally by Berkeley and Broome (1874) from material collected in Sri Lanka (Ceylon). Cordyceps muscicola Möller, a heterotypic synonymy of O. dipterigena, was described from 123 Fungal Diversity (2016) 80:1–270 185 Fig. 133 Neotruncatella endophytica (holotype). a, d Colonies in potato dextrose agar (PDA). b, e Colonies in oatmeal agar (OA). c, f Colonies in malt extract agar (MEA) (a–c obverse view, d–f reverse view). g, h Conidioma and peridium. i–k Conidia attached to conidiogenous cells. l 3-septate conidia with apical and basal appendages. Scale bars g = 50 lm, h–l = 20 lm material collected in Blumenau, Santa Catarina, Brazil. In a taxonomical perspective O. dipterigena corresponds to a species-complex, O. hemisphaerica differs from C. muscicola by its slightly smaller perithecia and asci (780–860 9 220–290 lm and 500–640 9 5–6 lm vs. 850–920 9 230–300 lm and 550–700 9 5 lm, respec- 123 186 tively). Furthermore, C. muscicola presents six stromata, smaller (9–13 mm) than those observed for O. hemisphaerica (12–20 mm); the host of C. muscicola was described as covered by mycelium found adhered to the abaxial region of the leaves (Möller 1901). Ophiocordyceps lacrimoidis Mafalda-Freire, Reck & Drechsler-Santos, sp. nov. Index Fungorum number: IF552123, Facesoffungi number: FoF02194, Fig. 120 Etymology: Referring to the lacrimoid form of the conidia. Holotype: FLOR 59552. Specimen found attached by mycelium on a twig, parasitic in true flies of Muscidae (host very damaged). Sexual morph capitated. Stromata 4–5 9 1 mm, simple, stipitate, 2 stromata arising from the thorax. Stipe 3–4 mm long, 1 mm wide, cylindrical, with a fertile apex, fleshy, robust, smooth, epidermal layer brown, compact, medullar region white to cream, soft, not compacted. Fertile head 1.2 mm long, 1.8–2.2 mm diam., discoid, pale to dark yellowish, ostioles inconspicuous on the upper surface. Ascomata obpyriform, slightly curved, 650–700 9 200–250 lm ( x = 675 9 225 lm, n = 40), completely immersed, yellow, thick-walled, perithecia easily detachable from the fertile region. Asci narrow cylindrical, 350–450 9 5 lm ( x = 400 9 5 lm, n = 40), apex thickened, 8-spored, hyaline. Apical cap 3–4 9 5–6 lm ( x = 3.5 9 5.5 lm, n = 40). Ascospores filiform, smooth, almost as long as asci, hyaline, more than 56 septa, easily breaking into partspores. Part-spores cylindrical, 8–14 9 2 lm ( x = 11 9 2 lm, n = 40), hyaline, smooth. Asexual morph Hymenostilbe-like. Synnemata cylindrical, 3 9 0.3 mm, simple, unique, smooth, orange brown, arising from the abdomen of the host. Conidiogenous cells phialidic, clavate, surface roughened, hyaline, pseudoparenchymatous, compactly arranged. Conidia lacrimoid, 4–5 9 3–5 lm ( x = 4.5 9 4 lm, n = 40), hyaline. Material examined: BRAZIL. Amazonas, Manaus, Reserva Florestal Adolfo Ducke, 3°000 1600 S 59°550 0700 W, on a dead fly attached on a twig, 2012, Mafalda-Freire FMF148 (FLOR 59552, holotype). Additional material examined (as Ophiocordyceps discoideicapitata): JAPAN, Yamagata, 8 July 1982 (TNS-F. 181545, NMNS); Iwate, Morioka, 18 July 2007, F. Ihara 07031 (personal collection of F. Ihara); F. Ihara 07032 (personal collection of F. Ihara); 11 August 2007, F. Ihara 07116 (personal collection of F. Ihara); F. Ihara 07117 (personal collection of F. Ihara); F. Ihara 07118 (personal collection of F. Ihara). Notes: Ophiocordyceps lacrimoidis is characterized by a robust short stipe, discoid fertile region, cylindrical synnemata and lacrimoid conidia. Additionally, the species parasitizes true flies of Muscidae, which are not covered by 123 Fungal Diversity (2016) 80:1–270 mycelium. According to phylogenetic analysis (Fig. 118), O. lacrimoidis is in a distinct lineage in the ‘‘O. sphecocephala clade’’, as sister of O. australis (Speg.) G.H. Sung et al. Morphologically, O. lacrimoidis is closely related to O. discoideicapitata (Kobayasi & Shimizu) G.H. Sung et al., but it can be differentiated by its darker fertile region with inconspicuous ostioles. Ophiocordyceps lacrimoidis slightly smaller perithecia than those of the O. discoideicapitata specimen studied by us (TNS-F. 181545: 700–820 9 270–280 lm) and similar to those described by Kobayasi and Shimizu (1982, 620–700 9 250–300 lm). The presence of synnemata and lacrimoid conidia was not observed for O. discoideicapitata. Furthermore, O. discoideicapitata was originally described from Japan, while the new species is described from Brazilian Amazon. Ophiocordyceps dipterigena and O. hemisphaerica also parasize flies, however the size of the stromata, and the morphology of fertile region and part-spores are very different (Table 1). Purpureocillium Luangsa-ard et al. The genus Purpureocillium was introduced by Luangsaard et al. (2011) with P. lilacinum (Thom) Luangsa-ard et al. as the type species, and it was reported as important pathogens in humans. Luangsa-ard et al. (2011) also showed that it belongs to Ophiocordycipitaceae, Hypocreales. Besides the type species, Purpureocillium comprises three species viz. P. atypicola (Yasuda) Spatafora et al., P. lavendulum Perdomo et al. and P. takamizusanense (Kobayasi) S. Ban, Azuma & Hirok. Sato (Index Fungorum 2016). Purpureocillium sodanum Papizadeh, Soudi, Wijayaw., Shahz.-Faz. & K.D. Hyde, sp. nov. Faces of Fungi number: IF552178; Facesoffungi Number: FoF02247, Fig. 122 Etymology: Named after isolation of this species from salt crystals. Holotype: IBRC-H 2020 Saprobic on salt crystals. Sexual morph Undetermined. Asexual morph Hyphae hyaline, smooth-walled, 1–2 lm wide. Conidiophores growing from the superficial mycelium very long, consisting of a stipe, bearing 6–8 lm long monoverticillate branches with terminal whorls of 2–3 phialides; stipe erect, septate, hyaline to pale purple-brown, rough-walled, profusely verrucose and up to 5 lm wide. Conidiophores growing from the aerial mycelium 25 to 40 nm long, irregularly branched, with 3–4 phialides per branch, septate, hyaline and smooth-walled. Phialides 5–13 9 2–3 mm, with a cylindrical basal portion tapering into a distinct neck up to 3 lm long. Conidia were in long dry chains, unicellular, subglobose with apiculate base or limoniform, 3.5–5.5 9 3–4.5 lm, smooth-walled, mostly Fungal Diversity (2016) 80:1–270 187 Fig. 134 Consensus tree resulting from a maximum likelihood analysis of combined LSU and ITS sequence alignment of taxa in Xylariales. RAxML bootstrap support values (MLB above 50 %) and Bayesian posterior probabilities (PP above 50 %) are given at the nodes (MLB/PP). The newly introduced sequences are in blue bold. The scale bar represents the expected number of changes per site. The tree was rooted to Vialaea mangiferae MFLUCC 12-0808 subhyaline, pinkish-purple in mass. Acremonium-like conidiophores were rarely observed, and when present, up to 50 lm long, simple, usually reduced to a cylindrical phialides, 9–22 9 1.5–2 mm. Chlamydospores and sexual morph were not observed. Culture characteristics: Colonies at 25 °C on MEA attained 28–30 mm diam. after 7 days incubation, consisting of a dense basal felt of numerous conidiophores giving a powdery texture, pale vinaceous (9B2), with sparse aerial mycelium, and producing a very faint yellow diffusible pigment. Colony features on CYA, CMA and PDA were similar to those observed on MEA, except that on PDA and CYA they were radially folded toward the periphery. Such a folding pattern was observed on MEA exclusively at 25 °C. Colonies on CYA were floccose and pale vinaceous (9A2). The optimum growth temperature was 30 °C (on MEA, 33–35 mm diam. after 7 days incubation) and the minimum was 20 °C. Colonies at 35 °C on MEA attained 23–25 mm diam. after 7 days incubation. Growth was insignificant at 37 °C and absent at 40 °C. Material examined: IRAN, Markazi Province, Mighan (Miqan) saline Lake, salt crystals, 2013, M. Papizadeh (IBRC-H 2020, holotype), ex-type culture IBRC-M 30175. Notes: In our phylogenetic analyses, the new strain clusters as a distinct clade from other species (Fig. 121). This clade is sister to the type species, but is separated from high bootstrap value (86 %). Hence, we introduce our strain as a new species based on DNA sequence analyses. P. sodanum is distinguished from other Purpureocillium species based on its growth at 30 up to 37 °C. Hypocreales incertae sedis Alfaria Crous et al. The monotypic genus Alfaria was introduced and typified by A. cyperi-esculenti Crous et al. The genus is placed in Hypocreales incertae sedis based on molecular data (Fig. 123). Alfaria spartii Senan., Camporesi & K.D. Hyde, sp. nov. Index Fungorum number: IF552303; Facesoffungi number: FoF02455, Fig. 124 Etymology: In reference to the host genus Spartium. Holotype: MFLU 16-1969. Saprobic on Spartium junceum L. Sexual morph Ascomata 220–250 lm high 9 190–275 lm diam. ( x = 216 9 123 188 Fig. 135 Cainia globosa (MFLU 14-0738, holotype). a Appearance of ascomata on host substrate as minute clypeate ostiolar dots. b Section through ascoma. The blackened clypeal area is narrow. c Peridium. d Papilla. e Pseudoparaphyses. f Ascus in Melzer’s reagent showing lightly bluing apical apparatus. g–j Immature and 123 Fungal Diversity (2016) 80:1–270 mature unitunicate ascospores in asci. k–o Ascospores. p Mucilaginous sheath. q Conidiomata on MEA. r Cross section of conidiomata. s, t Conidiogenous cells with conidia. u Conidia. Scale bars a, q, r = 200 lm, b = 100 lm, c, d, g–j, s–u = 50 lm, e, f, k–p = 20 lm Fungal Diversity (2016) 80:1–270 189 99/1 100/1 Seimatosporium pseudorosae MFLUCC 14-0468 Seimatosporium pseudorosarum MFLUCC 14-0466 Seimatosporium lichenicola MFLUCC 14-0623 Discostroma fuscellum NBRC 32680 69 Seimatosporium vaccinii ICMP 7003 Seimatosporium sp. MFLUCC 14-0454 Seimatosporium sorbi MFLUCC 14-0469 63 AB593735 Seimatosporium glandigenum 94/1 Discostroma fuscellum Emma Discostroma fuscellum NBRC 32625 94/1 Seimatosporium pseudocornii MFLUCC 14-0529 Seimatosporium cornicola MFLUCC 14-0448 Seimatosporium sp. IT1323 86/1 Seimatosporium cornii MFLUCC 14-0467 94/1 Seimatosporium cornii IT426 Discostroma cornii MFLUCC 14-1208 Discostroma tostum NBRC 32626 88/1 Seimatosporium parasiticum NBRC 32682 Seimatosporium physocarpi MFLUCC 14-0625 Seimatosporium vitis MFLUCC 14-0051 71/0.98 Discostroma botan DQ368629 64 70 Seimatosporium botan HMUC 316PD 80/1 Seimatosporium botan NBRC 104200T 60 Seimatosporium discosioides NBRC 104201 Seimatosporium pistaciae CBS 138865 96/1 Seimatosporium rosae MFLUCC 14-0621 Seimatosporium hypericinum NBRC 32647 85/1 Seimatosporium eucalypti CPC 156 Seimatosporium eucalypti CPC 159 Seimatosporium eucalypti CPC 157 56 98/1 Seimatosporium falcatum CPC 13578 Seimatosporium falcatum CPC 13580 Seimatosporium eucalypti CPC 158 Seimatosporium obtusum CPC 12935 87/1 Discostroma stoneae NBRC 32690 74/0.98 Seimatosporium kriegerianum NBRC 32679 Seimatosporium elegans NBRC 32674 Seimatosporium biseptatum CPC 13584 AB593736 Seimatosporium hakeae 60 Sarcostroma restionis CBS 118154 66 Sarcostroma restionis CBS 118153 Sarcostroma bisetulatum CBS 122695 56 Seimatosporium grevilleae ICMP 10981 64 Seimatosporium leptospermi ICMP 11845 Seimatosporium mariae NBRC 32681 Seimatosporium walkeri CPC 17644 Seimatosporium quercina MFLUCC 14-1198 51 98/1 Seimatosporium foliicola NBRC 32676 Seimatosporium ficeae MFLUCC 15-0519 Strickeria kochii C138 Strickeria kochii C143 88/1 Sarcostroma lomatiae CBS 118144 Seimatosporium rhombusii MFLUCC 15-0543 100/1 Discosia neofraxinea MFLU 15-0375 Discosia fraxinea NTIT469 63 Discosia brasiliensis NTCL097-2 51 74/1 Discosia brasiliensis NTCL095 Discosia brasiliensis NTCL094-2 Discosia sp. MAFF 236709 92 Discosia artocreas NBRC 8975 55 65 Discosia pini MAFF 410149 Discosia aff. brasiliensis NBRC 104198 Discosia sp. MAFF 242783 Discosia pseudoartocreas CPC 21117 Discosia sp. MFLU:14-0299A Discosia pseudoartocreas CBS 136438 Discosia aff. artocreas MAFF 242776 92/1 Adisciso yakushimense MAFF 242774 76/0.96 Adisciso yakushimen NBRC 104194T Adisciso tricellulare NBRC 32705 64 100/1 Adisciso tricellulare MAFF 237478 51 Discosia sp. MAFF 242778 Discosia sp. MFLU 14-0299 Discosia sp. MAFF 242785 Discosia sp. MFLU 14-0298 Discosia sp. MAFF 242777 100/1 Immersidiscosia eucalypti NBRC 104195 Immersidiscosia eucalypti NBRC 104196 72 Ciliochorella mangiferae MFLUCC 12-0310 82/0.98 Ciliochorella castaneae HHUF28800 Ciliochorella phanericola MFLUCC 14-0984 Pseudopestalotiopsis theae MFLUCC 12-0055 Seimatosporium Discosia Immersidiscosia Ciliochorella 10 Fig. 136 One of the 16 equally most parsimonious trees obtained from combined analyses set of LSU, ITS and b-tubulin sequence data (CI = 0.627, RI = 0.821, RC = 0.514, HI = 0.373). MP values ([50 %) resulting from 1000 bootstrap replicates and Bayesian posterior probabilities above 0.95 are given at the nodes. The tree is rooted to Pseudopestalotiopsis theae (MFLUCC 12-0055). Ex-type strains are in bold and newly introduced species is in blue 123 190 Fungal Diversity (2016) 80:1–270 Fig. 137 Ciliochorella phanericola on Phanera purpurea. a, b Symptoms on leaf. c Surface of fruiting bodies. d Cross section of the conidiostroma. e Peridium. f, g Mature conidia. h, i Colonies on MEA (I from below). Scale bars d = 300 lm, e = 30 lm and f, g = 20 lm 250 lm, n = 10), scattered, solitary, immersed, black, globose to subglobose, coriaceous, ostiolate. Ostiole lined with hyaline, filiform periphyses. Hamathecium comprises few, septate, hyphae-like, hyaline paraphyses. Peridium comprising thick-walled, brown cells of textura angularis. Asci 140–180 9 30–40 lm ( x = 155 9 37 lm, n = 20), 8-spored, unitunicate, oblong to fusiform, base sessile, apex oblong, with J-apical ring. Ascospores 30–35 9 10– 12 lm ( x = 32 9 11 lm, n = 20), overlapping bi-seriate, hyaline, oval to ellipsoid, unicellular, thick-walled, guttulate, guttules concentrated at the ends. Asexual morph Undetermined. 123 Culture characteristics: Colonies on MEA reaching 2 cm after 14 days at 18 °C, circular, umbonate, filiform, white, dense colonies, tightly attached to the media. Material examined: ITALY, Santa Sofia, Province of Forlı̀-Cesena [FC], Camposonaldo, on dead branch of Spartium junceum (Fabaceae), 13 September 2013, Erio Camporesi, IT 1452 (MFLU 16-1969, holotype; MFLU 16-1978, paratype), ex-type living culture, MFLUCC 13-0799. Notes: Alfaria spartii is the second species of genus Alfaria. In combined analyses of LSU and ITS sequence data, A. spartii clustered with A. cyperi-esculenti with high Fungal Diversity (2016) 80:1–270 support (Fig. 123). Alfaria spartii differs from A. cyperiesculenti in having large, oval ascospores with distinctly periphysate ostioles. Bionectriaceae Samuels & Rossman Bionectriaceae Samuels & Rossman was introduced by Rossman et al. (1999) to accommodate Bionectria and other allied genera. Members of this family are characterized by uniloculate hyaline to brightly coloured, soft perithecia with or without a well-developed stroma and unitunicate asci (Maharachchikumbura et al. 2016). Maharachchikumbura et al. (2016) accepted 39 genera and confirmed the familial placement in Hypocreales, Sordariomycetes in their phylogenetic analyses. Emericellopsis Beyma The genus Emericellopsis was introduced by van Beyma (1939-1940) with Emericellopsis terricola Beyma as the type species. Grum-Grzhimaylo et al. (2013) placed this genus in Bionectriaceae based on sequence analyses, but Maharachchikumbura et al. (2016) treated it as Hypocreales, genera incertae sedis. Based on habitats, species of Emericellopsis have been detected as saprobes. Moreover, Grum-Grzhimaylo et al. (2013) showed that Emericellopsis species can be classified into three categories based on their origins; marine, soda lakes, and terrestrial. Our new collection, Emericellopsis persica, falls into the terrestrial clade of this genus although detected in saline soils. Emericellopsis persica Papizadeh, Wijayaw, Soudi & K.D. Hyde, sp. nov. Index Fungorum number: IF552358; Facesoffungi Number: FoF 02517, Fig. 126 Etymology: Epithet taken from its isolation from Iran (formerly known as Persia). Holotype: IBRC-H 2022. Isolated from soil from lake. Sexual morph Ascomata dark brown, superficial on the substrate, globose, 280– 320 lm diam., non-ostiolate. Peridium 19–25 lm thick, multi-layered, pseudoparenchymatous, composed of 3–5 layers of compressed cells. Asci saccate, 15–18 lm diam., with thin deliquescent wall, soon dissolving, unitunicate, scattered irregularly in the ascocarp. Ascospores ellipsoid, pale brown, with uneven surfaces, 8–11 9 4–5 lm ( x = 9.5 9 4.5), surrounded by 3 longitudinal, hyaline, sharp appendages, width up to 1 lm. Asexual morph hyphomycetous, acremonium-like. Conidiophores mostly simple orthotropic. Conidiogenous cells 30–40 lm long, phialidic, tapering from 2–2.5 lm at the base to 0.5– 0.7 lm at the apex. Conidia narrowly ellipsoid, smoothsurfaced, 4.5–6.5 9 2 lm ( x = 5.5 9 2), hyaline, adhering in slimy heads. Chlamydospores mostly intercalary, 10–22 9 7–17 lm, hyaline. 191 Culture characteristics: Colonies on CYA agar fastgrowing, reaching 21–23 mm diam. in 7 days at 25 °C. On MEA (pH 6.5) growing slower, reaching 17–18 mm diam. in 7 days. Colonies orange-salmon-pink, later darkening in centre due to the formation of ascomata with tufted aerial mycelium. Reverse colourless. Exudate absent. Decumbent vegetative hyphae thin-walled, hyaline, 0.5–1.5 lm wide. Mycelium consisting of hyaline, smooth-walled, septate hyphae, 1–2 lm wide, often fasciculate. Material examined: IRAN, Lake Urmia, soil, 2011, M. Papizadeh, M.R. Soudi (IBRC-H 2022, holotype), ex-type culture IBRC-M 30046. Sequence: ITS sequence GenBank KX668543. The aligned dataset used in the analysis has been submitted with the TreeBase under the submission ID 19688 for the combined set of ITS and b-tub. Notes: In our phylogenetic analyses, Emericellopsis persica clusters as a distinct clade from other species. Emericellopsis donezkii and E. humicola are the closest species, but separated with high bootstrap support (100 %). Hence, we introduce our strain as a new species based on DNA sequence analyses (Figs. 125, 126). Meliolales Gäum. ex D. Hawksw. & O.E. Erikss. Hongsanan et al. (2015b) provided a monograph and a backbone tree for Meliolales. Meliolales is in Sordariomycetes, which also comprises the family Armatellaceae. A new tree incorporating Meliola citri-maximae and M. pseudosasae (Fig. 127) is provided below. Meliolaceae G.W. Martin ex Hansf. The family Meliolaceae was introduced by Martin (1941) without a Latin diagnosis and validated by Hansford (1946). It is typified by Meliola with M. nidulans (Schwein.) Cooke as the type species. At various times, this family has been placed in the orders Dothideales, Erysiphales, Meliolales, Myriangiales and Hypocreales (Martin 1941; Luttrell 1951, 1989; Roger 1953; Ainsworth et al. 1971; Müller and von Arx 1973; Yarwood 1973; Barr 1976; Eriksson 1981; Hawksworth et al. 1983). Kirk et al. (2001) introduced a new subclass, Meliolomycetidae (Sordariomycetes) for members of Meliolaceae, but without a description or diagnosis. The placement of Meliolomycetidae in Sordariomycetes was confirmed by Justavino et al. (2015) based on their phylogenetic analyses and validated in Maharachchikumbura et al. (2015). Ainsworth et al. (1971) and Eriksson and Hawksworth (1993) listed 50 genera in the family, but this was reduced to 25 genera (Hawksworth et al. 1995) and later 22 genera (Kirk et al. 2008). Lumbsch and Huhndorf (2010) placed Meliolaceae in the class Sordariomycetes with 26 genera. Hongsanan et al. (2015b) accepted only seven genera 123 192 Fungal Diversity (2016) 80:1–270 Fig. 138 Maximum parsimony (MP) majority rule consensus tree for the analyzed Neopestalotiopsis isolates based on a combined dataset of ITS, LSU, TUB and TEF sequence data. Values above or below the branches indicate maximum parsimony bootstrap equal or greater than 60 %. The tree is rooted with Pestalotiopsis trachicarpicola (OP068). The strain numbers are mentioned after the species names. The species obtained in this study are shown in blue bold and ex-type strains are in black bold Amazonia, Appendiculella, Asteridiella, Cryptomeliola, Endomeliola, Irenopsis and Meliola in this family. studies have provided sequence data from Meliola members (Gregory and John 1999, Justavino et al. 2015, Pinho et al. 2012, 2014, Hongsanan et al. 2015b), and phylogenetic placement of Meliola was clarified. However, there is still little sequence data for the entire subclass available in GenBank. Meliola Fr. The genus Meliola is typified by M. nidulans (Schwein.) Cooke. Meliola is the largest genus of Meliolaceae. Several 123 Fungal Diversity (2016) 80:1–270 193 Fig. 139 Neopestalotiopsis cocoës (holotype). a Leaf bright disease on coconut. b, c Conidiomata on host substrate. d Cross section of conidioma. e, f Conidiogenous cells with developing conidia. g Immature conidia. h–l Mature conidia. m Colony on PDA. Scale bars a = 2 mm, b = 500 lm, c = 250 lm, d = 100 lm, e, f = 20 lm, g–l = 15 lm Meliola citri-maximae X.Y. Zeng, K.D. Hyde & T.C. Wen, sp. nov. Index Fungorum number IF552271; Facesoffungi number: FoF02253, Fig. 128 Etymology: Referring to the host Citrus maxima (Burm. f.) Merr. Holotype: MFLU 14-0288. Epiphytes on the surface of living leaves. Colonies hypophyllous, scattered to dense, sometimes confluent. Hyphae superficial, brown, substraight, radiating outwardly, branched, septate, darker at septa, reticulate, with hyphal setae. Hyphal setae up to 320 lm long, dark brown, denticulate at the apex. Hyphopodia 20–22 9 8– 10 lm ( x = 21 9 9 lm, n = 20), 2-celled, brown, spathulate, form one or two near the septa, alternate to unilateral, sometimes opposite antrorse. Sexual morph Perithecia up to 250 lm, superficial, subdense, globose to subglobose, thick-walled, with ostiole, without perithecial setae. Peridium two strata, comprising hyaline inner cells and dark brown outer wall of textura angularis. 123 194 Fig. 140 Neopestalotiopsis musae (holotype). a Leaf bright disease on banana. b, c Conidiomata on host substrate. d Cross section of conidioma. e Peridium. f Apex. g, h Conidiogenous cells with 123 Fungal Diversity (2016) 80:1–270 developing conidia. i–j Mature conidia. k Germinating spore. l Colony on PDA. Scale bars d, e = 50 lm, f–h = 20 lm, i– j = 15 lm, k = 25 lm Fungal Diversity (2016) 80:1–270 Fig. 141 Pestalotiopsis sequoiae (MFLU 16-1489, holotype). a Herbarium specimen. b, c, Appearance of black coniodiomata on the host. d, e Vertical sections of conidiomata. f–h Conidiophores, 195 conidiogenous cells and developing conidia. i–m Conidia. Scale bars d = 100 lm, e = 50 lm, f–h = 10 lm, i–m = 5 lm 123 196 Hamathecium with evanescent paraphyses. Asci unitunicate, 2–3-spored, ovoid to ellipsoid when young, with short pedicel, asci wall attenuated or broken when mature, evanescent. Ascospores 42–48 9 16–21 lm ( x = 44 9 19 lm, n = 20), 2–3-seriate, cylindrical, hyaline at young state, becoming dark brown when mature, 3– 4-septate, constricted and darker at the septa, middle cell a little larger, rounded at both ends, smooth-walled. Asexual morph Phialides 15–22(–25) 9 7–9 lm ( x = 20 9 8 lm, n = 10), ampulliform, form one at top of hyphal cell, alternate or unilateral, sometimes mixed with capitate hyphopodia. Conidia undetermined. Material examined: THAILAND, Chiang Rai, Horticulture Research Centre, on living leaves of Citrus maxima (Rutaceae), 12 March. 2014, Xiang-Yu Zeng (MFLU 14-0288, holotype) Notes: Species of Meliolaceae found on Citrus include Amazonia butleri, Meliola amyridis, M. camelliae and M. citricola. Morphologically, the new collection is typical to the genus Meliola in having hyphal setae, but differs from M. camelliae and M. citricola as it has 2–3-spored asci instead of 8-spored asci in M. camelliae and shorter setae, larger perithecia and larger ascospores than M. citricola. It is most similar to M. amyridis in having hypophyllous and dense colonies, alternate hyphopodia, and ascospore size, but differs in having shorter setae, larger perithecia and hyphopodia. Phylogenetically, our new collection is related to M. brachyodonta and M. trichostroma. The sequence data of M. brachyodonta and M. trichostroma, which are unpublished, were provided by Pinho et al. (2013). However, these two species were found on Croton curranii (Euphorbiaceae) and Psidium guajava (Myrtaceae) respectively, which are on the different host family from this new collection. Although LSU phylogenies did not fully resolve the affinities of M. citri-maximae with other known Meliola species, we suggest it is a new taxon. Further analyses should target a more variable region such as ITS or btubulin, as the LSU gene is too conserved to differentiate species (Jeewon et al. 2002, 2003). Meliola pseudosasae I. Hino, Bull. Faculty of Agriculture, Yamaguchi University 9: 882 (1958) Facesoffungi number: FoF02458, Fig. 129 Epiphytes on the upper surface of leaves of Sasa borealis (Hack.) Makino & Shibata. Superficial hyphae branched, septate, darker at the septa, brown, with hyphopodia, hyphal setae present. Hyphopodia 10–11 lm diam. ( x = 10.5 lm, n = 20), head cell capitate, 22–25 lm long ( x = 23 lm, n = 20), alternate or opposite on hyphae, near to hyphal septum, 2-celled, upper cell cylindrical and 123 Fungal Diversity (2016) 80:1–270 Fig. 142 Consensus tree resulting from a Bayesian analysis of the c LSU and ITS sequence of taxa in Sordariomycetes. RAxML bootstrap support values (MLB above 50) and Bayesian posterior probabilities (PP above 90 %) are given at the nodes (MLB/PP). The newly introduced sequences are in blue bold. The scale bar represents the expected number of changes per site. The tree was rooted to Sordaria fimicola (HKUCC3414) curly, brown. Hyphal setae 170–210 lm high 9 9–12 wide lm ( x = 180 9 10 lm, n = 10), arising from hyphae, comprising 2–3 arms, with 2–4-branches in each arm, acute and hyaline at the apex, septate, brown to dark brown, smooth-walled. Sexual morph Ascomata 97–135 lm high 9 105–160 lm diam. ( x = 120 9 140 lm, n = 10), superficial on the surface of hosts, solitary to gregarious on superficial hyphae, globose to subglobose, thick-walled, ascomatal setae and appendages absent, surface of ascomata verrucose. Peridium 17–20 lm comprising dark brown cells of textura angularis when viewed in squash mounts, with two strata, outer stratum thick-walled, dark brown cells of irregular textura angularis, and inner stratum of flattened, hyaline cells. Hamathecium not observed. Asci not observed. Ascospores 45–52 lm high 9 18–23 wide lm ( x = 50 9 22 lm, n = 10), 2-seriate, oblong to broadly cylindrical, hyaline to dark brown, 4-septate, constricted and darker at the septa, smooth-walled, ends rounded. Asexual morph Undetermined. Material examined: CHINA, Xishuangbanna, on living leaves of Sasa borealis (Pinaceae), 22 November 2015, R Phookamsak Xb002 (MFLU 16-2136, in KIB, reference specimen designated here). Notes: Our fresh specimen is similar to Meliola pseudosasae both in host (Sasa borealis) and morphology. It has hyphal setae with 2–3 arms and with 2–4-branches at the apex, superficial hyphae with hyphopodia and 4-septate, brown ascospores as in the protologue. We were unable to isolate our specimen in culture, thus, DNA was extracted from ascomata and ascospores directly. Phylogenetic analysis (Fig. 127) indicate that our collection clusters with members of Meliolaceae within the subclass Meliolomycetidae, Sordariomycetes. Meliola pseudosasae is related to M. clerodendricola Henn. strain, which was published as a reference specimen by Hongsanan et al. (2015b). Asteridiella, Appendiculella and Endomeliola species clustered with Meliola species. Thus, further molecular data are needed to clarify if they are polyphyletic. We designate our collection as a reference specimen (sensu Ariyawansa et al. 2014b). Xylariales Nannf. For the order Xylariales, we follow Daranagama et al. (2016). Fungal Diversity (2016) 80:1–270 197 123 198 Apiosporaceae K.D. Hyde et al. The monophyletic family Apiosporaceae was introduced by Hyde et al. (1998), and is typified by the genus Apiospora. Apiospora typified by A. montagnei Sacc. is characterized by apiospores and a basauxic, Arthriniumlike conidiogenesis (Samuels et al. 1981; Hyde et al. 1998; Bahl 2006, Senanayake et al. 2015). Arthrinium Kunze Crous and Groenewald (2013) provided a monograph on this genus and Index Fungorum (2016) listed 60 species. The older name Arthrinium was conserved as it is a more commonly encountered name and more frequently used in literature (Crous and Groenewald 2013). We provide an updated tree here (Fig. 130). Arthrinium paraphaeospermum Senan., & K.D. Hyde, sp. nov. Index Fungorum number: IF552137, Facesoffungi number: FoF02459, Fig. 131 Etymology: In reference to the large conidia and conidiogenous cells and morphological similarity to the A. phaeospermum. Holotype: MFLU 16-1974 Saprobic on bamboo. Sexual morph Undetermined. Asexual morph Mycelium consisting of smooth, hyaline, branched, septate, 3–5 lm diam. hyphae. Conidiophores reduced to conidiogenous cells. Conidiogenous cells 25– 30 9 4–6 lm ( x = 27 9 4.7 lm), basauxic, aggregated in clusters on hyphae, hyaline, smooth, elongated, conical to ampulliform. Conidia 10–19 lm diam., ( x = 13 lm) brown, smooth, somewhat granular, globose to ellipsoid in surface view, with a median scar, lenticular in side view, with pale equatorial slit. Sterile cells 20–30 9 9– 13 lm ( x = 24 9 11 lm), forming on solitary loci on hyphae, brown, finely roughened, ellipsoid to clavate. Culture characteristics: Colonies on MEA flat, circular, smooth, spreading, with fluffy aerial mycelium, mycelia not tightly attached to the surface, dirty white, with patches of iron-grey to black due to sporulation. Material examined: THAILAND, Chiang Mai Prov., Doi Inthanon, Hwy 1009 at 25 km marker, N18°32.540 , E98°33.510 , alt. 1076 m, on dead clumps of Bambusa sp. L. (Poaceae), 4 November 2012, I. C. Senanayake, CHUNI 33, (MFLU 16-1974, holotype), ex-type living culture, MFLUCC 13-0644. Notes: Arthrinium paraphaeospermum is phylogenetically closely related to A. rasikravindrii and A. hydei (Fig. 130). However A. paraphaeospermum is phylogenetically distinct with high support (97/0.9, Fig. 130) and morphologically differs in having ellipsoid to clavate, brown, sterile tissues, elongated, conical conidiogenous cells and conidia with a median scar. 123 Fungal Diversity (2016) 80:1–270 Bartaliniaceae Wijayaw. et al. The family Bartaliniaceae was introduced by Senanayake et al. (2015) based on analysis of ITS and 28S rDNA sequence data, and includes Bartalinia, Broomella, Dyrithiopsis, Hyalotiella, Truncatella and Zetiasplozna. The polyphyletic genera were placed in this family based on similarities in morphology and their phylogeny (Senanayake et al. 2015; Maharachchikumbura et al. 2016). Members of Bartaliniaceae including Truncatella species produce secondary metabolites with potential use in biotechnological applications including control of plant diseases (Flores-Bustamante et al. 2010; Zhao et al. 2015c). Truncatella was established by Steyaert (1949). The genus was previously placed in Amphisphaeriaceae, Xylariales, a family including 41 genera, although some genera are uncertain position (Lumbsch and Huhndorf 2010). The primary reason for introducing a separate genus is to accommodate a species which is distinct in having 3-septate conidia (Steyaert 1949; Maharachchikumbura et al. 2012). Molecular data has been used to evaluate pestalotiod fungi, including species of Truncatella (Jeewon et al. 2002; Lee et al. 2006; Maharachchikumbura et al. 2012). During a study on the Amphisphaeriales from an Abies firma leaf in Korea, a new genus that differed morphologically and phylogenetically (Fig. 132) from related species was isolated and is described as new. Neotruncatella Hyang B. Lee & T.T.T. Nguyen, gen. nov. MycoBank number: MB 817422; Facesoffungi number: FoF02460 Etymology: The genus name ‘‘Neotruncatella’’ refers to the similarity with Truncatella Endophyte of Abies firma. Sexual morph Undetermined. Asexual morph Conidiomata globose to subglobose, black. Conidiogenous cells hyaline, ampulliform to subcylindrical, formed from the inner cells of the peridial wall. Conidia fusiform with rounded ends, yellow to yellowish-brown at maturity, mostly 3-septate; apical appendages short. Type species: Neotruncatella endophytica Hyang B. Lee, P.M. Kirk, K.D. Hyde, S.S.N. Maharachch., & T.T.T. Nguyen Neotruncatella endophytica Hyang B. Lee, P.M. Kirk, K.D. Hyde, S.S.N. Maharachch., & T.T.T. Nguyen, sp. nov. MycoBank number: MB 816958; Facesoffungi number: FoF02478, Fig. 133 Etymology: endophytica, referring to the endophytic habitat Holotype: EML-AS5-1 Fungal Diversity (2016) 80:1–270 199 Fig. 143 Anthostomella ravennica (holotype). a, b Appearance of ascomata on host. c Cross section of ascoma. d Peridium. e Paraphyses. f–h Asci (f note the apical ring bluing in Melzer’s reagent). i–k Ascospores without visible germ slits. Scale bars a = 200 lm, b, c = 100 lm, d = 50 lm, f–k = 10 lm Endophyte of plants. Sexual morph Undetermined. Asexual morph Conidiomata globose to subglobose, black, 75–190 9 75–215 lm diam. Conidiogenous cells hyaline, phialidic, ampulliform to subcylindrical, formed from the inner cells of the peridial wall. Conidia fusiform with rounded ends, yellow to yellowish-brown at maturity, mostly 3-septate, 25–34 9 3–5 lm; apical cell slightly yellow, thin-walled, bearing a single apical appendage, 3–5 123 200 123 Fungal Diversity (2016) 80:1–270 Fungal Diversity (2016) 80:1–270 b Fig. 144 Anthostomella thailandica (holotype). a–c Appearance of ascomata on host surface. d Ascoma in cross section. e Peridium. f, g Mature asci. h Apical ring bluing in Melzer’s reagent. i–n Immaturemature ascospores. Scale bars a = 1000 lm, b–c = 500 lm, d = 100 lm, e–g = 20 lm, h = 5 lm, i–n = 10 lm (av. 4) lm long; apical appendages short, 4.5–6.5 lm long; two median cells yellow to yellowish-brown; second cell from the base 7.5–11.5 lm long, third cell 6.5–10.5 lm long; basal cell conic or with a truncate base, slightly yellowish-white, 5–7 lm long, basal appendages present or absent, if appendages present, very shortly at base, 1–3.5 lm long. Culture characteristics: Colonies on PDA reaching 82–85 mm diam. at 25 °C after 14 days, initially greenishglaucous, but finally whitish with a white margin; reverse yellowish with irregular margin; on OA powdery white. The isolate was observed to grow over a wide range of temperatures with average growth rates of EML-AS5-1 on PDA, MEA, and OA of 6.8 mm, 5.8 mm, and 6.5 mm per 24 h, respectively. Optimal growth was observed around 23 °C–27 °C, slow growth was observed at below 15 °C, and no growth at 35 °C (Fig. 132). Sporulation is excellent on oatmeal agar and malt extract agar. Notes: Neotruncatella is morphologically distinct from Truncatella and Bartalinia by having narrower conidia with only a shorter apical appendage. In the phylogenetic trees, the ITS and 28S rDNA sequences (KX216520 and KX216518, respectively) of the strain formed a separate branch distinct with them, showing it represents a new species. In addition, ITS based phylogenies resulted in a clearly defined monophyletic subclade for our novel genus basal to Bartalinia (Fig. 132). Morphological comparison also reveals that Neotruncatella is characterized by a centric basal appendages as compared to Bartalinia. Material examined: REPUBLIC OF KOREA, Jeonnam Province, garden of the Chonnam National University located in Gwangju (35°100 N 126°550 E), from a Abies firma leaf, 3 August 2015 (EML-AS5-1, holotype); EMLAS5-1 (ex-type) at Culture Collection of National Institute of Biological Resources (NIBR), Incheon, Chonnam National University Fungal Collection (CNUFC-EMLAS5-1) and Jena Microbial Resource Collection (University of Jena and Leibniz Institute for Natural Product Research and Infection Biology, Jena, Germany) (JMRC:SF:012333). Cainiaceae J.C. Krug The family Cainiaceae was introduced to accommodate species with a characteristic apical ring comprising a series of rings (Krug 1978). This family comprises Amphibambusa, Atrotorquata, Arecophila, Cainia, Ceriophora, 201 Reticulosphaeria, Seynesia and Ommatomyces (Kohlmeyer and Volkmann-Kohlmeyer 1993; Hyde 1996; Senanayake et al. 2015, Maharachchikumbura et al. 2016). Rhabdospora-like species have been reported as the asexual morph of this family (Müller and Corbaz 1956). Cainia Arx & E. Müll. Cainia is typified by C. graminis (Niessl) Arx & E. Müll., and comprises eight species (Index Fungorum 2016). Members of Cainia occur on grasses and are characterized by immersed ascomata, cylindrical asci with a characteristic J? apical ring with series of rings, brown, two-celled, ascospores with longitudinal striations, and ascospores surrounded by a mucilaginous sheath (Krug 1978; Senanayake et al. 2015). An updated tree for Cainia is provided (Fig. 134). Cainia globosa Senan., Camporesi & K.D. Hyde, sp. nov. Index Fungorum number: IF552136, Facesoffungi number: FoF02461, Fig. 135 Etymology: In reference to the globose ascospores. Holotype: MFLU 14-0738. Saprobic on standing culms of Anthoxanthum odoratum L. Sexual morph Ascomata 250–360 lm high 9 210– 315 lm diam. ( x = 326 9 264 lm, n = 10), solitary to scattered, immersed beneath clypeus, dark brown to black, globose to subglobose, uniloculate, coriaceous, with a central ostiole. Peridium 20–25 lm wide, composed of inner, thick-walled, compressed, hyaline cells of textura angularis and outer, dark brown to black cells of textura angularis. Hamathecium comprising 3–4 lm wide, septate, guttulate, sometimes branched, hyaline paraphyses. Asci 175–190 9 40–50 lm ( x = 188 9 46 lm, n = 20), 8-spored, unitunicate, cylindric-clavate, straight, short pedicellate, with a layered, apical ring, bluing lightly in Melzer’s reagent. Ascospores 30– 35 9 14–17 lm ( x = 33 9 16 lm, n = 20), uni-seriate, ellipsoid to oval, rounded at both ends, hyaline when immature, becoming pale brown to dark brown when mature, medianly 1-septate, not constricted at the septum, guttulate when immature, striate, surrounded by a mucilaginous sheath. Asexual morph Conidiomata pycnidia, superficial, solitary, formed at the colony margins. Conidiophores reduced to conidiogenesis cells. Conidiogenous cells 4–7 9 1.5–3 lm, ( x = 5.5 9 2.4 lm), holoblastic, cylindrical, hyaline, unbranched, septate. Conidia 7–10 9 3.5–5 lm ( x = 8 9 4.4 lm, n = 20), globose to oval, 0–1-septate, brown, striate. Culture characteristics: Colonies on MEA reaching 2 cm after 14 days at 18 °C, circular, dense, white, velvety, radiating towards the slightly undulate edge, not 123 202 Fungal Diversity (2016) 80:1–270 Fig. 145 Phylogram generated from Bayesian analysis based on combined LSU and ITS sequence data from species of Sordariomycetes. Maximum parsimony/likelihood bootstrap support values greater than 50 % and Bayesian posterior probabilities greater than 0.90 are shown in above and below. The new isolates are in red and other extype strains are in bold. The tree is rooted with Sordaria fimicola pigmented, after 4 weeks, small superficial, pycnidia formed mainly at margin of the colonies. Material examined: ITALY, Province of Forlı̀-Cesena, Santa Sofia, near San Paolo in Alps, on dead culms of Anthoxanthum odoratum (Poaceae), 2 May 2013, E. Camporesi, IT 1217 (MFLU 14-0738, holotype), ex-type living culture, MFLUCC 13-0663. Notes: Cainia globosa is morphologically distinct from other species in having ascospores with small guttules in each cell, a very lightly bluing bi-lobed apical ring and 123 Fungal Diversity (2016) 80:1–270 Fig. 146 Sporidesmium pyriformatum (MFLU 15-1155, holotype). a Colonies. b–e Fruiting body. f Conidiophores. g, h Conidiogenous cell. i–n Conidia. o Germinated conidia on PDA medium. p, q 203 Culture, p from above, q from below. Scale bars a = 100 lm, b–f, o = 30 lm, g, h = 20 lm, i–n = 10 lm 123 204 Fig. 147 Sporidesmium aquaticivaginatum (MFLU 15-1159, holotype). a Colonies on wood. b, c Conidiophores with conidia. d Conidium developing on the apex of conidiophore. e Conidiophores. 123 Fungal Diversity (2016) 80:1–270 f–j Conidia. k Germinated conidium on PDA medium. l, m Culture, l from above, m from below. Scale bars a = 100 lm, b–k = 30 lm Fungal Diversity (2016) 80:1–270 Fig. 148 Sporidesmium olivaceoconidium (MFLU 15-1175). a Substrate. b Colonies on wood. c, d Conidiophores. e Conidiogenous cell. f Fruiting body. g–k Conidia. l Germinated conidium on PDA 205 medium. m, n Culture, m from above, n from below. Scale bars b = 50 lm, c–e, h–k = 15 lm, f = 20 lm, g = 30 lm, l = 40 lm 123 206 Fig. 149 Distoseptispora multiseptata (MFLU 15-1144, holotype). a Colonies on wood. b–e Conidiophores and conidia. f–j Conidia. k Conidiophore. l Germinated conidium on PDA medium. m, n 123 Fungal Diversity (2016) 80:1–270 Culture on MEA medium, m from above, n from below. Scale bars a = 200 lm, b–g = 50 lm, h, i = 40 lm, j, k = 20 lm, l = 100 lm Fungal Diversity (2016) 80:1–270 1-septate, striate conidia. Cainia globosa clustered together with C. graminis and C. anthoxanthis. However C. globosa separates from these species with high support (85/ 0.9).The asexual morph of Cainia observed from a pure culture of C. desmazieresii was reported by Müller and Corbaz (1956) as Rhabdospora-like. However, Krug (1978) was unable to obtain the asexual morph from C. desmazieresii in culture (Kang et al. 1999). Here we observed a coelomycetous asexual morph from the culture (Fig. 135). Pestalotiopsidaceae Maharachch. & K.D. Hyde Pestalotiopsidaceae was introduced by Maharachchikumbura and Hyde (2015), which possesses pestalotiopsis-like asexual morphs (Senanayake et al. 2015, Maharachchikumbura et al. 2016). Six genera belong to Pestalotiopsidaceae; Ciliochorella, Monochaetia, Neopestalotiopsis, Pestalotiopsis, Pseudopestalotiopsis and Seiridium (Senanayake et al. 2015, Maharachchikumbura et al. 2016). Most taxa in Pestalotiopsidaceae are phytopathogens that cause a variety of diseases in plants and some are often isolated as saprobes or endophytes and are widely distributed throughout tropical and temperate regions (Guba 1961; Barr 1975; Nag Raj 1993; Maharachchikumbura et al. 2014a, b, 2016). Ciliochorella Syd. Ciliochorella was introduced by Sydow & Mitter (1935) with C. mangiferae Syd. as the type species. Seven species of Ciliochorella (C. bambusarum Shanor, C. buxifoliae Allegr., Eliades & Aramb., C. castaneae Munjal, C. eucalypti T.S. Viswan, C. indica Kalani, C. mangiferae Syd., C. splendida Nag Raj & R.F. Castañeda) are recorded in Index Fungorum (2016). Only two strains of Ciliochorella have sequence data (LSU) in GenBank (Tangthirasunun et al. 2015). Tangthirasunun et al. (2015) provided sequence data for the reference specimen of C. mangiferae found on dead leaves in Thailand. Ciliochorella phanericola C. Norphanphoun, T.C. Wen & K.D. Hyde, sp. nov. Index Fungorum number: IF552264; Facesoffungi Number: FoF02462, Fig. 137 Etymology: In reference to the host Phanera, and Latin cola meaning loving. Holotype: MFLU 14-0776. Pathogen or saprobic on leaf. Sexual morph Undetermined. Asexual morph Coelomycetous. Conidiomata semiimmersed, circular areas, carbonaceous, black, mostly aggregated, sometimes solitary, in cross section 1000–1200 lm diam., 170–200 lm high. Peridium comprising a few to several layers of cell of textura angularis, with inner most layer thin, 207 pale-brown, outer layer dark brown to black. Conidiophores reduced to conidiogenous cells. Conidiogenous cells enteroblastic phialidic, formed from the inner most layer of wall, hyaline to pale brown, smooth. Conidia (12.5–)13–15 9 2.8– 3.5(–4) lm ( x = 15 9 3.7 lm, n = 30), allantoid to subcylindrical, 2-septate, hyaline to pale brown, smooth-walled; apical cell with 2 tubular apical appendages, 15–23 lm ( x = 20 lm), unequal; basal appendage 9–11.5 lm ( x = 11 lm). Sexual morph Undetermined. Culture characteristics: Colonies on MEA, reaching 2.5 cm diam. after 7 days at 25 °C, producing dense mycelium, circular, margin rough, white, without aerial mycelium. Material examined: THAILAND, Chiang Rai, Mae Fah Luang University, on dead leaves of Phanera purpurea (L.) Benth. (Leguminosae), August 2014, Ausana Mapook (MFLU 14-0776, holotype; KUN, isotype); ex-type-living cultures, MFLUCC 14-0984, KUMCC. Notes: Phylogenetic analyses based on maximum parsimony and Bayesian analyses of LSU, ITS and TUB sequence data, provide good evidence that our strain belongs in Ciliochorella. Phylogenetic analysis confirms C. phanericola is a new species with high support (Fig. 136). However, C. castaneae differs mainly by 0–2 septate, branched conidiophores occasionally reduced to conidiogenous cells (Allegrucci et al. 2011). Ciliochorella phanericola, has conidia of a similar size (12–17 9 2–4 lm) as C. mangiferae, the latter has fusiform, 3-septate conidia, while those of C. phanericola are allantoid to subcylindrical, 2-septate with longer basal and apical appendages. Neopestalotiopsis Maharachch. et al. Neopestalotiopsis species are important plant pathogens, causing post-harvest diseases, fruit rots, and leaf blights in plants worldwide. Pestalotiopsis was divided into three genera by Maharachchikumbura et al. (2014b), viz Neopestalotiopsis, Pestalotiopsis, and Pseudopestalotiopsis with Neopestalotiopsis being distinguished from Pseudopestalotiopsis and Pestalotiopsis by its versicolorous median cells. Conidiophores in Neopestalotiopsis are indistinct and often reduced to conidiogenous cells (Xu et al. 1999; Das et al. 2014, b; Maharachchikumbura et al. 2013, 2014b; Hyde et al. 2014; Jayawardena et al. 2015). Neopestalotiopsis cocoës Norphanphoun, T.C. Wen & K.D. Hyde, sp. nov. Index Fungorum number: IF552232; Facesoffungi Number: FoF02364, Fig. 139 Etymology: The specific epithet cocoes refers to the host. 123 208 Fungal Diversity (2016) 80:1–270 Fig. 150 Distoseptispora tectonae (holotype). a Colonies on dead branch. b, c Conidia attached to conidiophores on host. d–f Conidia. g, h Conidia attached to conidiogenous cells and conidiophores. Scale bars d–h = 20 lm Holotype: MFLU 15-0220. Pathogenic on Cocos nucifera L. Sexual morph Undetermined. Asexual morph Conidiomata 170– 123 260 9 140–190 lm ( x = 250 9 180 lm, n = 10), acervuli, with basal stroma and lateral wall of 1–2 layers, thin, wall cells pale brown, textura angularis. Conidiophores Ellis (1958), McKenzie (1995) Dead culms of Pennisetum purpureum on Freycinetia sp. Paler, rounded apex (38–)50–75(–85) 9 11–15 Up to 175 9 6–7 Di. leonensis 7–12-distoseptate Malaysia Shoemaker and White (1985), Réblová (1999) Decayed wood of Fagus sylvatica Hyaline, hemisphaerical apex (80)350–500 9 15–18 28–46 9 8–10 Di. adscendens 80-distoseptate Hungary Su et al. (2016a) On decaying wood submerged in stream Paler towards the apex 125–250 9 13–15 21–33 9 5.5–6.5 D. fluminicola 17–34-distoseptate China This study T. grandis Slightly paler, rounded to annellidic apex (83–)148–225(–360) 9 (10–)11–12(–13) Up to 110 9 5–11 Di. tectonigena 20–46-distoseptate Thailand This study Tectona grandis Slightly paler, rounded apex 20–28-distoseptate (90–)130–140(–170) 9 (11–)13–14(–16) Up to 40 9 4–6 Di. tectonae Apex of conidia Conidia (lm) Conidiophores (lm) Conidia septation Thailand 209 Species Table 2 Synopsis of Distoseptispora tectonae, D. tectonicola, D. adscendens and D. leonensis Country Host Reference Fungal Diversity (2016) 80:1–270 reduced to conidiogenous cells. Conidiogenous cells enteroblastic, discrete, simple, short, filiform. Conidia 19– 22.5 9 7.5–9.5 lm ( x = 21 9 9.3 lm, n = 30), fusiform to ellipsoid, straight to slightly curved, 4-septate; basal cell conic, hyaline, thin and smooth-walled, 3.4–4.6 lm long ( x = 4.5 lm); three median cells 13.4–14.5 lm long ( x = 14 lm), brown to pale brown, versicolored, verruculose; second cell from base pale brown to olivaceous, 3.8–5.5 lm ( x = 5.2 lm); third cell darker brown to olivaceous, 4.2–5.5 lm ( x = 5.5 lm); fourth cell darker brown, 3.6–4.9 lm ( x = 4–3 lm); apical cell 2.1–3.2 lm long ( x = 2.3 lm), hyaline, cylindric to subcylindric; apical appendages 14.9–21 lm long ( x = 19.6 lm), 2–3 (mostly 3), basal appendage present (1 or rarely absent), filiform 3.2–8.1 lm ( x = 6 lm). Culture characteristics: Colonies on MEA, reaching 4 cm diam. after 7 days at 25 °C, producing dense mycelium, circular, rough margin white. Material examined: THAILAND, Chiang Rai, Mushroom Research Foundation, on leaf of Cocos nucifera L. (Arecaceae), 4 February 2015, K.D. Hyde (MFLU 15-0220, holotype; KUN, isotype); ex-type-living cultures, MFLUCC 15-0152, KUMCC. Notes: Neopestalotiopsis cocoës was associated with leaf blight disease of Cocos nucifera. In the phylogenetic analyses Neopestalotiopsis egyptiaca Ismail et al., is sister to our strain (Fig. 138). However, this species differs from N. cocoës in having larger conidia (22.5–28 lm). The conidia of N. cocoës are also wider (7.5–9.5 lm) than those of N. egyptiaca (6–7.5 lm) (Jayawardena et al. 2016) (Fig. 139). Neopestalotiopsis musae Norphanphoun, T.C. Wen & K.D. Hyde, sp. nov. Index Fungorum number: IF552233, Facesoffungi Number: FoF02363, Fig. 140 Etymology: Named after the host genus on which the fungus occurs. Holotype: MFLU 16-1279. Pathogenic on Musa sp. Sexual morph Undetermined. Asexual morph Conidiomata 175–180 9 110–160 lm ( x = 175 9 140 lm), acervuli, with basal stroma and lateral wall 1–3 cells thick; the wall cells hyaline, textura angularis. Conidiophores reduced to conidiogenous cells. Conidiogenous cells enteroblastic, discrete, simple, short, filiform. Conidia 18.6–25 9 4.1–5 lm ( x = 20.5 9 4.5 lm), fusiform to ellipsoid, straight to slightly curved, 4-septate; basal cell conic, hyaline, thin and smoothwalled, 3.2–5.2 lm long ( x = 3.5 lm); three median cells 12.1–16 lm long ( x = 15 lm), hyaline, versicolored, verruculose; second cell from base pale brown to olivaceous, 4.1–6.1 lm ( x = 5 lm); third cell pale brown to olivaceous, 3.2–6.1 lm ( x = 5.5 lm); fourth cell pale 123 210 brown to olivaceous, 3.3–6 lm ( x = 4.5 lm); apical cell 3.6–5.2 lm long ( x = 3.7 lm), hyaline, cylindric to subcylindric; apical appendages 16.3–25 lm long ( x = 16 lm), 2–3 (mostly 3), basal appendage filiform, 4.6–10.3 lm long ( x = 5 lm). Culture characteristics: Colonies on PDA, reaching 5 cm diam. after 10 days at 25 °C, producing dense mycelium, circular, rough margin white, after 2 weeks, flat or effuse on the surface, without aerial mycelium. Material examined: THAILAND, Chiang Rai, Pha Chang, on leaf of Musa sp. (Musaceae), 4 February 2015, Chada Norphanphoun (MFLU 16-1279, holotype; KUN, isotype); ex-type-living cultures, MFLUCC 15-0776, KUMCC. Notes: Molecular analysis provides good evidence that our strain belongs to Neopestalotiopsis and is closest to N. honoluluana Maharachch. et al. and N. zimbabwana Maharachch. et al. (Fig. 138). However, N. honoluluana and N. zimbabwana has longer and wider conidia ( x = 28 9 8.3 lm, = 25.3 9 7.7 lm, respectively) with N. honoluluana having three dark median cells, unlike N. musae which has smaller conidia ( x = 20.5 9 4.5 lm), and pale-brown median cell of conidia (Maharachchikumbura et al. 2014b). Pestalotiopsis sequoiae W.J. Li, Camporesi & K.D. Hyde, sp. nov. Index Fungorum number: IF552215; Facesoffungi number: FoF02352, Fig. 141 Etymology: Named after the host genus Sequoia. Holotype: 16-1489. Saprobic on dead stem of Sequoia sempervirens (Lamb. ex D. Don) Endl. (Cupressaceae). Sexual morph Undetermined. Asexual morph Coelomycetous. Conidiomata 160–305 lm diam. 9 162–326 lm high, black, solitary to aggregated or confluent, subperidermal in origin, erumpent at maturity, pycnidioid, globose to subglobose, unilocular, glabrous, straw-coloured with dark center; wall 10–20 lm wide, composed of thin-walled, hyaline cells of textura angularis to textura globulosa. Conidiophores reduced to conidiogenous cells. Conidiogenous cells 4–24 lm long 9 2–4 lm wide, hyaline, enteroblastic, lageniform to subcylindrical, arising from the cells lining the inner wall of the pycnidium. Conidia 21–30 9 7.5–10 lm ( = 25 9 8.5 lm, n = 30 lm), fusiform, 4-septate, straight or slightly curved, bearing tubular, unbranched, flexuous appendage at both ends, basal cell 2.9–5.7 lm ( x = 4.3 lm) long, hyaline, obconic with a truncate base, 3 median cells, together 14.7–20 lm long (second cell from base 4.1–7 ( x = 5.6 lm, third cell 5.4–6.9 lm ( x = 6.2 lm), fourth cell 4.6–6.7 lm ( x = 5.6 lm), cylindrical, pale brown to brown and concolourous, septa and periclinal wall darker, apical cell 2.9–4.8 lm 123 Fungal Diversity (2016) 80:1–270 ( x = 4 lm) long, hyaline, conical, with mostly 4 apical appendages, 3–17 lm ( x = 11 lm) long, with independent loci of origin along upper half of the apical cell, often forming aggregated crest, basal appendage 4–11 lm ( x = 6 lm) long, single, unbranched. Culture characteristics: Colony on PDA reaching 40–50 mm diam. in 7 days, with circular margin, white and fluffy, dense, aerial mycelium on the surface, reverse similar in colour. Material examined: ITALY. Province of Forlı̀-Cesena [FC]), Castrocaro Terme, on dead stem of Sequoia sempervirens (Cupressaceae), 20 October 2012, Erio Camporesi, IT-832 (MFLU 16-1489, holotype); ex-type living culture, MFLUCC 13-0399, ICMP; IT-832B (HKAS 95026, isotype); living culture, KUMCC 15-0642. Notes: Phylogenetically, based on megablast search of NCBIs GenBank nucleotide database, the closest hit using ITS sequence is Pestalotiopsis hollandica (GenBank KM199328; Identities = 548/548(100 %); Gaps = 0/ 548(0 %)), Followed by uncultured fungus clone (GenBank = KP889337; Identities = 549/550(99 %); Gaps = 0/550(0 %)), and P. monochaeta (GenBank = KM199327; Identities = 547/547(100 %); Gaps = 0/547(0 %)). Closest hits using the TEF sequence yielded highest similarity to P. hollandica (GenBank = KM199481; Identities = 237/ 238(99 %); Gaps = 1/238(0 %)), Pestalotiopsis sp. (GenBank = KP781881; Identities = 228/230(99 %); Gaps = 2/230(0 %)), P. verruculosa (GenBank = JX399061; Identities = 233/238(98 %); Gaps = 1/238(0 %)). Morphologically, the conidia of Pestalotiopsis sequoiae is smaller than those of P. hollandica [(25–)25.5–33(–34) 9 8.5–10(–10.5) lm], P. monochaeta [(25–)27–40(–42) 9 7–11(–11.5) lm] and P. verruculosa [(28–35 9 9–11 lm ( = 30:6 9 10:3 lm)]. Based on morphology and phylogeny (Fig. 138), Pestalotiopsis sequoiae is introduced as novel species in Pestalotiopsis. Xylariaceae Tul. & C. Tul. The family Xylariaceae currently comprises 87 genera (Maharachchikumbura et al. 2016). Xylariaceous members are well-known for their secondary metabolite production (Stadler 2011). Xylariaceae has been traditionally classified into two subfamilies (Chesters and Greenhalgh 1964; Ju and Rogers 1996; Bitzer et al. 2008). In this study we introduce new species to genus Anthostomella. Anthostomella ravennica Daranagama, Camporesi & K. D. Hyde, sp. nov. Index Fungorum number: IF552286; Facesoffungi number: FoF02418, Fig. 143 Etymology: ‘‘ravennica’’ refers to the province from where it was first collected Holotype: MFLU 16-0972 Fungal Diversity (2016) 80:1–270 Fig. 151 Distoseptispora tectonigena (holotype). a, b Colonies on dead twig. c Conidiophores. d, e Conidia attached to conidiophores. f–i Conidia. j, k Percurrently proliferating apex of conidia. 211 l Germinated conidium. m Colony on PDA after 7 months. Scale bars a = 200 lm, b = 100 lm, d–h = 20 lm 123 212 123 Fungal Diversity (2016) 80:1–270 Fungal Diversity (2016) 80:1–270 b Fig. 152 Maximum likelihood phylogenetic tree generated by RAxML (GTR?G model) based on LSU sequence data. ML/MP values ([50 %) resulting from 1000 bootstrap replicates are given at the nodes and branches with Bayesian posterior probabilities greater than 0.90 are in bold. The original isolate or specimen numbers are noted after the species names. The tree is rooted to Colletotrichum asianum. Newly generated sequence is in red and other ex-type strains are in bold Saprobic on dead stem of Ammophila arenaria (L.) Link. Sexual morph Ascomata 170–190 9 180–200 lm ( x = 175 lm 9 192 lm, n = 10), immersed, visible as black, irregular to dome-shaped areas, coriaceous, solitary, scattered, in cross section globose, with inconspicuous ostiole. Ostiole black, papillate. Peridium 30–50 lm wide ( x = 35 lm, n = 10), with two cell layers, outwardly comprising thick-walled, compressed, dark brown cells of textura angularis and inwardly comprising a few layers thin-walled, hyaline cells of textura angularis. Paraphyses 3.3–3.8 lm wide at base ( x = 3.5 lm, n = 30), shorter than the asci, numerous, filamentous, septate. Asci 116– 127 9 11–13.6 lm ( x = 124 9 12.8 lm, n = 20), 8spored, unitunicate, cylindrical, short pedicellate, with conspicuous, J?, discoid, apical ring, 0.7–1.5 9 0.2– 0.8 lm ( x = 1.2 9 0.5 lm, n = 20). Ascospores 14– 17 9 7–8 lm ( x = 16.2 9 7.6 lm, n = 20), uni-seriate, inequilaterally ellipsoidal, with one convex surface, pointed ends, light brown-dark brown, smooth-walled, without germ slit. Asexual morph Undetermined. Culture characteristics: Colonies on OA 9 cm diam. after 4 weeks at 27 °C, white at the margins, pale yellowish at the center; reverse yellowish to cream, colony azonate, cottony appearance, dense. Material examined: ITALY, Ravenna Province, Lido di Dante, on dead stems of Ammophila arenaria (L.) Link, 28 January 2015, E. Camporesi, IT 2358 (MFLU 16-0972, holotype), ibid. HKAS, isotype; ex-type living culture, MFLUCC 15-0012, KUMCC. Notes: Anthostomella ravennica is morphologically reminiscent of A. zongluensis K.D. Hyde. However, the new species can be distinguished as the latter has larger ascomata with a central ostiolar canal, a thin peridium, while A. ravennica has irregular or sometimes domeshaped ascomata, with an inconspicuous ostiole. Ascospores in A. zongluensis have conspicuous mucilaginous sheaths and asci have a distinct, J?, wedged-shaped apical ring, while ascospores in A. ravennica lack mucilaginous sheaths and asci have a J?, discoid apical ring which is inconspicuous. Due to these morphological differences we designate our collection as a new species. Molecular data also support A. ravennica to be phylogenetically distinct (Figs. 142, 143) 213 Anthostomella thailandica Daranagama & K.D. Hyde, sp. nov. Index Fungorum number: IF552287; Facesoffungi number: FoF02419, Fig. 144 Etymology: ‘‘thailandica’’ refers to the country it was first collected. Holotype: MFLU 16-0971 Saprobic on leaves of grasses. Sexual morph Ascomata 150–200 9 140–155 lm ( x = 167 9 148 lm, n = 10), immersed, visible as black, irregular areas, coriaceous, solitary, sometimes clustered in groups of two, scattered, in cross section subglobose. Ostiole inconspicuous, without conspicuous papilla. Peridium 14–22 lm wide ( x= 17 lm, n = 10), comprising two cell layers, outwardly comprising thin-walled, loosely arranged, light brown cells of textura globosa and inwardly comprising a few layers of thin-walled, hyaline cells of textura angularis. Paraphyses 4.2–6.5 lm wide at base ( x = 4.9 lm, n = 30), as long as asci, few, filamentous, septate. Asci 87–118 9 14.1– 17.8 lm ( x = 92.6 9 15.2 lm, n = 20), 8-spored, unitunicate, cylindrical-clavate, apedicellate, with conspicuous, J ?, discoid apical ring, 3.1–3.8 9 1.1–1.9 lm ( x = 3.5 9 1.5 lm, n = 20). Ascospores 12–17 9 5.5– 9 lm ( x = 15.4 9 7.2 lm, n = 20), uni-seriate, equilateral ellipsoidal, with broad ends, olivaceous, smooth-walled, with one large, central guttule, without germ slit. Asexual morph Undetermined. Culture characteristics: Colonies on OA 9 cm diam. after 4 weeks at 27 °C, white at the margins, pale yellowish at the center; reverse yellowish to cream, colony azonate, cottony appearance, dense. Material examined: THAILAND, Chiang Rai Province, Mae Fah Luang University garden, on dead leaves of a grass, 12 August 2015, D.A. Daranagama, KM24 (MFLU 16-0971, holotype), ibid. HKAS, isotype; ex-type living culture, MFLUCC 15-0017, KUMCC. Notes: Our collection is reminiscent of both A. zongluensis K.D. Hyde and A. consanguinea (Ces.) Sacc. However, A. zongluensis has ascospores with more parallel sides, minutely verruculose walls and ascomata with periphysate ostiolar canals (Lu and Hyde 2000). Ascospores of A. thailandica are similar in size to those of A. consanguinea (Ces.) Sacc. However, ascospores of A. consanguinea have verruculose walls and a short, straight germ slit, while those in A. thailandica have a smooth wall and lack germ slits. In the phylogenetic analysis A. thailandica clusters in a separate clade with A. formosa Kirschst., A. conorum (Fuckel) Sacc., A. rubicola Speg. ex Sacc. & Trotter and A. obesa Daranagama, E. Camporesi & K.D. Hyde. 123 214 Fungal Diversity (2016) 80:1–270 Fig. 153 Paracapsulospora metroxyli (holotype). a Appearance of ascomata on host substrate. b Close up of ascomata. c Peridium. d Section of ascoma. e–j Asci. k–q Ascospores. r Germinated ascospore. s Culture characters on MEA. Scale bars a = 1 mm, b = 200 lm, c = 10 lm, d = 100 lm, e = 50 lm, f–j = 20 lm, k– r = 5 lm Sordariomycetes incertae sedis Sporidesmiaceae Fr. The family Sporidesmiaceae was introduced by Fries (1849), but the taxon has not been widely used in modern classification. The family is typified by Sporidesmium Link with S. atrum Link as the type species. Su et al. (2016a) treated Ellisembia as a synonym of Sporidesmium based on molecular data and placed in the presently monotypic Sporidesmiaceae. Based on molecular data, Sporidesmiaceae shows a sister relationship to Papulosaceae and Trichosphaeriaceae (Fig. 145). 123 Fungal Diversity (2016) 80:1–270 215 Fig. 154 Phylogenetic tree of the Clavulinopsis and Ramariopsis obtained by analyses from rDNA sequences. Taxa with two accession numbers were analyzed by concatenated ITS and partial LSU rDNA; only the partial LSU rDNA was used to analyze the other individuals. Support values (from top) are maximum likelihood (ML) and Bayesian analyses. Sequences obtained in this study are in boldface. Only support values of at least 50 % are shown. The tree was rooted with Clavaria acuta Sporidesmium Link The genus Sporidesmium was typified by S. atrum. Ellis (1958) considered S. ehrenbergii be the same as the type. Sporidesmium is characterized by unbranched or sparingly branched conidiophores; monoblastic or percurrently proliferating conidiogenous cells with broad scar; euseptate, rostrate conidia, sometimes with a mucilaginous apex (Shenoy et al. 2006; Seifertk et al. 2011). Sporidesmium is morphologically similar to the distoseptate genus Ellisembia. However, Su et al. (2016a) considered the euseptate/distoseptate difference between Sporidesmium and Ellisembia to have little taxonomic 123 216 Fungal Diversity (2016) 80:1–270 Fig. 155 Clavulinopsis aurantiaca (holotype). a Basidiomata. b, c Hyphae with clamps. d Basidia, one clamped at the base. e Basidia with sterigmata and basidiospores. f Basidiospores with apiculus. Scale bars a = 1 cm, b = 20 lm significant and that did not appear to be supported by any molecular data. Sporidesmium is shown to be polyphyletic based on phylogenetic studies (Shenoy et al. 2006; Su et al. 2016a). Sporidesmium pyriformatum J. Yang & K.D. Hyde, sp. nov. Index Fungorum number: IF552227; Facesoffungi number: FoF02245, Fig. 146 Etymology: Referring to the pyriform conidia. Holotype: MFLU 15-1155 Saprobic on submerged wood in a stream. Colonies on substrate superficial, effuse, dark brown, hairy. Mycelium mostly immersed, composed of branched, septate, hyaline to pale brown hyphae. Sexual morph Undetermined. Asexual morph Conidiophores macronematous, mononematous, solitary or caespitose, cylindrical, straight or slightly flexuous, smooth, 2–5-septate, brown, paler towards the apex, 65–190 9 3–5.5 lm ( x = 118.5 9 4 lm, n = 20). Conidiogenous cells holoblastic, 123 integrated, lageniform, terminal, brown to olivaceous, 9–24.5 9 4–7 lm, darkened and truncate at the apex, with up to three lageniform percurrently proliferating. Conidia acrogenous, solitary, pyriform or obclavate, 3–4-euseptate, the lower 3–4 cells olivaceous to brown, with pale brown to subhyaline apical cell, darkened at the septa, 18.5–32.5 9 8–15.5 lm ( x = 25.5 9 12 lm, n = 20). Culture characteristics: Conidia germinating on PDA within 24 h. Germ tubes produced from the apex. Colonies on MEA reaching 10–15 mm diam. after 2 weeks at 25 °C, white in the middle, pale yellow at edge, with dense white mycelium on surface, sparser to the edge; in reverse with a yellow middle with yellowish irregular margin. Material examined: THAILAND, Prachuap Khiri Khan Province, Hua Hin, stream flowing outside Kaeng Krachan National Park, on submerged wood, 25 December 2014, Jaap van Strien Site4-26-3 (MFLU 15-1155, holotype); extype living culture, MFLUCC15-0620; ibid. (MFLU 15-1162, paratype). Fungal Diversity (2016) 80:1–270 217 Fig. 156 Ramariopsis atlantica (holotype). a Basidiomata. b Hyphae with clamps. c Hyphal ends resembling gloeocystidia. d Basidiospores, one of them germinating. Scale bars a = 1 cm, b = 20 lm Note: Phylogenetic analysis of combined LSU and ITS sequence data, supports the placement of our taxon within Sporidesmium and reveals a close relationship (Fig. 145) to S. parvum (S. Hughes) M.B. Ellis and S. fluminicola H.Y. Su & K.D. Hyde. We therefore introduced this as a new species, Sporidesmium pyriformatum. The conidial shape of S. pyriformatum resembles those of S. penzigii Cooke & Ellis, S. jasminicola M.B. Ellis, S. bambusinum N.D. Sharma, S. fraxini-paxianae Jian Ma & X.G. Zhang, S. submersum H.Y. Su & K.D. Hyde, S. parvum and S. fluminicola. However, the conidia of the new taxon are larger than those of S. parvum (18–24 9 8–9 lm), but smaller than those of the other taxa that listed above. Sporidesmium pyriformatum is distinguished from the newly introduced taxa S. submersum and S. fluminicola in having much longer conidiophores and fewer septa (Su et al. 2016a). Sporidesmium aquaticivaginatum J. Yang & K.D. Hyde, sp. nov. Index Fungorum number: IF552229; Facesoffungi number: FoF02369, Fig. 147 Etymology: Referring to the aquatic habitat and the mucilaginous sheath. Holotype: MFLU 15-1159 Saprobic on decaying plant twigs. Sexual morph Undetermined. Asexual morph Colonies effuse, olivaceous, hairy. Mycelium partly immersed, partly superficial on the substrate. Conidiophores macronematous, mononematous, solitary or sometimes caespitose, cylindrical, straight or slightly flexuous, smooth, dark brown, triangular or globose at the base, 1–3-septate, 60–125 9 4–6 lm ( x = 82.5 9 5 lm, n = 15). Conidiogenous cells holoblastic, monoblastic, integrated, terminal, determinate or with one percurrent proliferation, cylindrical, smooth, mid to dark brown. Conidia acrogenous, solitary, dry, olivaceous to pale brown, paler at the apex, obclavate, tapering gradually towards the apex, smooth, straight or curved, 6–10-distoseptate, sometimes with a mucilaginous sheath, 49.5–80.5 9 10.5–14 lm ( x = 66.5 9 12 lm, n = 20), rounded and 2.3–3.5 lm at the apex, with a thick, flat, colourless, sheath extending from the tip to half way down, truncate and 3.5 9 5 lm at the base with conspicuous darkened scars. Culture characteristics: Colonies on PDA reaching 10–15 mm diam. after 4 weeks at 25–30 °C, colony from above, dark greenish in the middle becoming greyish-green at the umbonate margin; from below, greyish-green in the centre and the third layer, dark greenish to black at the second layer and the edge; medium raised to umbonate in the middle. 123 218 Material examined: THAILAND, Prachuap Khiri Khan Province, Hua Hin, stream flowing outside Kaeng Krachan National Park, on submerged wood, 25 December 2014, Jaap van Strien, Site4-44-2 (MFLU 15-1159, holotype); ex-type living culture, MFLUCC15-0624; ibid. (HKAS950 46, isotype). Note: The combined LSU and ITS phylogenetic analyses indicate Sporidesmium aquaticivaginatum represents a sister taxon to S. olivaceoconidium within the Sporidesmiaceae (Fig. 145). In conidial shape and sheath, Fig. 157 Phylogram resulting from the RAxML (Stamatakis 2014) analysis of ITS sequence data. Bootstrap values greater than 50 % are indicated above branches. The names in blue represent the species of 123 Fungal Diversity (2016) 80:1–270 Fig. 158 a, f Cortinarius fulvescens 04-935 (neotype, H). b, g c C. fulvescentoideus 03-1675 (H). c, i C. pseudobulliardioides 11-452 (holotype, H). d, j C. tenuifulvescens 04-572 (holotype, H). e, h C. nymphatus DBB21430 (UC). Scale bar 10 mm. (a–d Photographs by K. Liimatainen, e by D. Bojantchev. f–j Drawings by T. Niskanen and I. Kytövuori.) S. aquaticivaginatum resembles S. bambusae M.B. Ellis, S. minigelatinosa Matush., S. novozymium W.P. Wu. and S. palmicola W.P. Wu. Conidia in these species are characterized by a globose to subclavate, mucilaginous sheath Cortinarius presented in this paper and the specimens in boldface are type specimens of the species. The tree is rooted with Obtusi species Fungal Diversity (2016) 80:1–270 219 123 220 at the apex. The conidiophores of S. aquaticivaginatum (60–125 9 4–6 lm) are obviously longer than those of S. bambusae (30–70 9 4–5 lm), S. minigelatinosa (40–100 9 4–5 lm) and S. palmicola (20–35 9 4–6 lm), but shorter than S. novozymium (150–200 9 6–8 lm). The conidia of S. hainanense (40–50 9 10–13 lm) and S. minigelatinosa (45–52 9 8–12 lm) are smaller than the conidia of S. aquaticivaginatum (49.5–80.5 9 10.5–14 lm). Moreover, among all these species, S. hainanense and S. palmicola are described as having euseptate conidia while in others the conidia are euseptate and distoseptate. The olivaceous conidia with a basal scar of S. aquaticivaginatum is clearly distinguishable from the brown or dark brown conidia of S. bambusae, S. minigelatinosa, S. novozymium and S. palmicola. Sporidesmium olivaceoconidium J. Yang & K.D. Hyde, sp. nov. Index Fungorum number: IF552230; Facesoffungi number: FoF02368, Fig. 148 Etymology: Referring to the olivaceous conidia. Holotype: MFLU 15-1175. Saprobic on decaying plant twigs. Sexual morph Undetermined. Asexual morph Colonies effuse, olivaceous, hairy. Mycelium partly immersed, partly superficial on the substrate. Conidiohores macronematous, mononematous, solitary or sometimes caespitose, cylindrical, straight or slightly flexuous, smooth, dark brown, slightly swollen at the base, 1–3-septate, 25–55 9 3–4 lm ( x = 40 9 3.5 lm, n = 20). Conidiogenous cells holoblastic, monoblastic, integrated, terminal, determinate, cylindrical, lageniform, smooth, mid to dark brown, 11– 15 9 3–5 lm ( x = 13 9 4 lm, n = 20). Conidia acrogenous, solitary, dry, olivaceous to pale brown, paler towards the tip, obclavate, smooth, straight or slightly curved, 6– 10-septate, sometimes with a rounded mucilaginous sheath at the tip, 25–50 9 6–10 lm ( x = 40 9 8 lm, n = 20), rounded at the apex, truncate at the base. Culture characteristics: Conidia germinating on PDA within 24 h and germ tube produced from the apex. Colonies on MEA slow-growing, reaching 5–10 mm diam. in 14 days, with dense white mycelium in the center, becoming sparse at the edge, folded in the middle; in reverse, brown in the middle and paler, smooth in the margin. Material examined: THAILAND, Chiang Rai Province, stream flowing in Tham Luang Nang Non Cave, on submerged wood, 25 November 2014, Jing Yang YJ-14 (MFLU 15-1175, holotype), ex-type living culture, MFLUCC 15-0380, GZCC 16-0008. Notes: The placement of Sporidesmium olivaceoconidium is revealed by molecular data within Sporidesmiaceae as sister taxon to S. aquaticivaginatum (Fig. 145). However, 123 Fungal Diversity (2016) 80:1–270 Fig. 159 RPB2-based phylogram generated from maximum likeli- c hood analysis (RAxML) depicting the placement of Rhodocybe indica, R. luteobrunnea and R. griseoaurantia within the genus Rhodocybe. Values at nodes indicate bootstrap support. BS values C50 % are indicated above or below the nodes, new species are in blue bold. The tree is rooted with Tricholoma flavovirens (KC816997) and Catathelasma imperiale (KC816994) the phylogenetic tree contains a single long branch of the taxa S. olivaceoconidium and S. aquaticivaginatum with all other branches being short. It requires more collections for the future study of these two taxa. Sporidesmium olivaceoconidium is morphologically similar to S. aquaticivaginatum. It differs from S. aquaticivaginatum by the shorter conidiophores and smaller conidia. Distoseptisporaceae K.D. Hyde & McKenzie The family was introduced by Su et al. (2016a) and we follow this here. We introduce a new species of Distoseptispora and provide an updated tree (Fig. 145). Distoseptispora K.D. Hyde et al. We follow Su et al. (2016a) Distoseptispora multiseptata J. Yang & K.D. Hyde, sp. nov. Index Fungorum number: IF552206; Facesoffungi number: FoF02244, Fig. 149 Etymology: Referring to the multi-septate conidia. Holotype: MFLU 15-1144. Saprobic on wood submerged in stream. Colonies effuse, dark olive-green, hairy or velvety. Mycelium mostly immersed, comprised of branched, septate, smooth, hyaline to pale brown hyphae. Sexual morph Undetermined. Asexual morph Conidiophores macronematous, mononematous, solitary, brown, 2–3-septate, straight or slightly flexuous, erect, slightly tapering distally, truncate at the apex, olive-green to mid brown, 23–65 9 4.5–8.5 lm ( x = 41.5 9 6 lm, n = 20). Conidiogenous cells holoblastic, monoblastic, integrated, terminal, brown, determinate, cylindrical. Conidia acrogenous, solitary, obclavate, rostrate, multi-distoseptate, tapering towards the apex, dark olivaceous green, 95–290 lm ( x = 160 lm, n = 30) long, 11–20 lm ( x = 16 lm, n = 30) wide at the broadest part, rounded and 5.5–9 lm ( x = 7.5 lm, n = 30) wide at the apex, basal cell subcylindrical, truncate and 3–5.5 lm ( x = 4 lm, n = 30) wide at the base. Conidial secession schizolytic. Culture characteristics: Conidia germinating on PDA within 24 h. Germ tubes produced from both ends. Colonies on MEA reaching 10–15 mm diam. after 2 weeks at 25 °C, greyish-green on the surface, with dense, fluffy mycelium folded in the middle, dark green in reverse, with smooth margin. Material examined: THAILAND, Prachuap Khiri Khan Province, Hua Hin, a stream flowing outside Kaeng Fungal Diversity (2016) 80:1–270 221 123 222 Krachan National Park, on decaying submerged wood, 25 December 2014, Jaap van Strien, Site4-4-2 (MFLU 15-1144, holotype), ex-type culture, MFLUCC 15-0609, GZCC; ibid. (HKAS 95045, isotype). Notes: Distoseptispora multiseptata is placed within the recently established genus Distoseptispora and it is wellsupported by both morphology and molecular data. Distoseptispora multiseptata clusters with D. fluminicola, D. tectonae and D. tectonigena. Distoseptispora multiseptata however, is distinguished by its dark olivaceous green conidia, while the conidia are yellowish to dark reddish brown in D. fluminicola, D. tectonae and D. tectonigena. The new species also differs from D. fluminicola and D. tectonae in having larger conidiophores and conidia, but smaller than those of D. tectonigena (Su et al. 2016a). Distoseptispora tectonigena is the only species in the genus having percurrently proliferating conidiogenous cells at the apex of conidiophores. Distoseptispora multiseptata resembles D. aquatica in conidia shape and colour, but differs by its larger conidia (Su et al. 2016a). Distoseptispora tectonae Doilom & K.D. Hyde, sp. nov. Index Fungorum number: IF552223; Facesoffungi number: FoF01877, Fig. 150 Etymology: Name refers to the host genus Tectona on which the fungus was collected. Holotype: MFLU 15-3417. Saprobic on dead twig and branch of Tectona grandis L.f. Sexual morph Undetermined. Asexual morph Colonies on natural substrate, superficial, numerous, hairy, dark brown, scattered, single or in groups. Conidiophores up to 40 lm long, 4–6 lm wide, macronematous, mononematous, simple, erect to slightly curved, unbranched, 2–4septate, slightly constricted at the septa, pale brown to dark brown, cylindrical. Conidiogenous cells 7.5–9.5 9 3.5– 5 lm, monoblastic, integrated, terminal, cylindrical. Conidia (90–)130–140(–170) 9 (11–)13–14(–16) lm ( x = 128 9 14 lm, n = 20), 3.5–5.5 lm wide at the protruding truncate base (rostrum), 20–28-distoseptate, flexuous, cylindric-obclavate, elongate, straight or slightly curved, rounded at the apex, obconically truncate at the base, verruculose, dark reddish brown, slightly paler towards the apex, thick-walled, smooth, secession schizolytic. Culture characteristics: Conidia germinating on PDA within 24 h. Germ tubes produced at the apex and septa of conidia. Colonies on MEA reaching 8–10 mm diam. after 7 days in the dark at 25 °C ( x = 8.9 mm, n = 5), undulate, irregular shape, flat or effuse at the edge, velvety, brownish-grey (4E2) from above, olive brown (4F3) from below. Material examined: THAILAND, Utaradit Province, on dead twig of Tectona grandis (Lamiaceae), 29 December 2012, M. Doilom (MFLU 15-3417, holotype), ex-type 123 Fungal Diversity (2016) 80:1–270 living culture MFLUCC 12-0291, MKT 030, ICMP 21156; Phrae Province, Song District, on dead branch of T. grandis, 30 October 2011, M. Doilom, (MFLU 15-3416), living culture MFLUCC 12-0288, MKT 025, ICMP 21154. Notes: Distoseptispora tectonae is introduced here as a novel species based on its morphological and phylogenetic differences from known Distoseptispora species. Phylogenetically, D. tectonae lies in a subclade together with D. fluminicola McKenzie et al. 2016 and D. tectonigena sp. nov. (this study), but in a distinct lineage with support 67 %MPBS, 55 % MLBS and 1.00 PP (Fig. 145). The conidia of D. tectonae ((90–)130–140(–170) 9 (11–) 13–14(–16)) are shorter and slightly narrower than those of D. fluminicola (125–250 9 13–15), D. adscendens ((80)350–500 9 15–18), and shorter and wider than D. tectonigena ((83–)148–225(–360) 9 (10–)11–12(–13)), but longer than those of D. leonensis ((38–)50–75 (–85) 9 11–15). The conidia of D. tectonae also have more septa than those of D. leonensis, but fewer than those of D. adscendens, D. fluminicola and D. tectonigena. In addition, the conidiophores of D. tectonae are shorter than those of D. adscendens, D. leonensis and D. tectonigena, but longer than D. fluminicola (Table 2). Distoseptispora tectonigena Doilom & K.D. Hyde, sp. nov. Index Fungorum number: IF552224; Facesoffungi number: FoF01878, Fig. 151 Etymology: Name refers to the host genus Tectona on which the fungus was collected, gena are used for a second species from the same host. Holotype: MFLU 15-3418. Saprobic on dead twig of Tectona grandis. Sexual morph Undetermined. Asexual morph Colonies on natural substrate, superficial, numerous, hairy, dark brown, scattered, single or in groups. Conidiophores up to 110 lm long, 5–11 lm wide, macronematous, mononematous, simple, erect, straight to slightly curved, septate, slightly constricted at the septa, pale brown to dark brown, 2–6septate, cylindrical. Conidiogenous cells 4–10 9 2–5.5 lm, monoblastic, integrated, terminal, lageniform or cylindrical, brown, smooth, with lageniform or doliiform. Conidia (83–) 148–225(–360) (rostrum included) 9 (10–)11–12(–13) lm thick ( x = 170 9 11 lm, n = 30), 2.5–6 lm wide at the truncate base (rostrum), older conidia 20–46-distoseptate, flexuous, cylindric-obclavate, elongated, straight or slightly curved, verruculose, rounded at apex, sometimes percurrently proliferating 5–10 times at apex, obconically truncate at base, dark reddish-brown and slightly paler towards the apex, thick-walled, smooth, secession schizolytic. Culture characteristics: Conidia germinating on PDA within 24 h. Germ tubes produced at the apex and other cells of conidia. Colonies on MEA reaching 6–13 mm Fungal Diversity (2016) 80:1–270 223 Fig. 160 Rhodocybe indica (CAL 1323, holotype). a Basidiospores. b Basidium. c Pseudocystidia. d Stipitipellis. e Pileipellis with pileocystidia. f Basidiocarp in the field. Scale bars a–e = 10 lm f = 10 mm (a–e Photo by K.P. Deepna Latha, f Photos by K.N. Anil Raj) diam. after 7 days in the dark at 25 °C ( x = 9.9 mm, n = 5), edge entire to undulate, flat or effuse, velvety, aerial, medium sparse, grey (7F1) from both above and below. Material examined: THAILAND, Chiang Rai Province, Mae Lao District, on dead twig of Tectona grandis (Lamiaceae), 12 March 2012, M. Doilom (MFLU 15-3418, holotype), ex-type living cultures MFLUCC 12-0292, MKT 033, ICMP 21157. Notes: Although phylogenies reveal a close affinity between D. fluminicola, D. tectonae and D. tectonigena (Fig. 145). Distoseptispora tectonigena differs from D. fluminicola and D. tectonae by its conidiophores and conidial dimensions, conidial septation and apex of conidia as well as by phylogenetic analyses. Conidiophores of D. tectonigena are longer than those of D. tectonae and D. fluminicola. Its conidia are longer and narrower than D. tectonae, shorter and narrower than D. fluminicola. The conidia have more septa than those of D. tectonae and D. fluminicola (Table 2). The conidial apex of D. tectonigena is sometimes rounded like that of D. tectonae, but sometimes D. tectonigena produces an annellidic apex (Table 2). Sordariomycetidae, genera incertae sedis Paracapsulospora Konta. & K. D. Hyde, gen. nov. Saprobic on dead Metroxylon sagu (Arecaceae). Sexual morph Ascomata solitary, immersed, uniloculate, globose to subglobose, surrounded by a dark brown layer of peridium cells. Peridium composed of outer layers of black, thickwalled cells of textura angularis inner layers hyaline and thin-walled. Asci, 8-spored, unitunicate, cylindrical, long pedicellate. Ascospores uni-seriate, oblong to cylindrical, unicellular, hyaline, with two guttules, thin-walled, smoothwalled, have appendages with a distinct mucilaginous sheath. Asexual morph Undetermined. Note: Paracapsulospora is introduced to accommodate a single species P. metroxyli which was collected from Fig. 161 Rhodocybe luteobrunnea (CAL 1322, holotype). Basidiocarps in the field. Scale bar 10 mm (Photo by K.N. Anil Raj) Metroxylon in Thailand. The genus is morphologically similar to Capsulospora and Xylochrysis in having cylindrical asci with uni-seriate and oblong to cylindrical, aseptate ascospores. Paracapsulospora is most similar to Capsulospora in having immersed ascomata, with uni-seriate, hyaline, oblong to cylindrical, aseptate ascospores. Paracapsulospora differs from Xylochrysis in forming immersed ascomata, with hyaline ascospores, while Xylochrysis forms ascostromata erumpent through host tissue by long neck and forms paraphyses, with pale brown ascospores However, these two genera can be distinguished as Paracapsulospora has appendages at both ends of ascospores and does not produce paraphyses at the centrum, whereas Capsulospora forms paraphyses buts lacks appendages. Phylogenetic analyses show that 123 224 Fungal Diversity (2016) 80:1–270 Fig. 162 Rhodocybe luteobrunnea (CAL 1322, holotype). a Basidiospores. b Basidium. c Pseudocystidia. d Pileipellis with encrustations. e Stipitipellis. Scale bars a–e = 10 lm (Photos by K.N. Anil Raj) Paracapsulospora is close to the orders Magnaporthales and Amplistromatales but cannot be confidently assigned to these order due to weak support (Fig. 152). Based on the limited sequences data in this group, we could not clarify the natural placement of our genus. Thus, we treat the new genus in the Sordariomycetes genera incertae sedis. Type species: Paracapsulospora metroxyli Konta. & K. D. Hyde Paracapsulospora metroxyli Konta & K. D. Hyde. sp. nov. Index Fungorum number: IF552257; Facesoffungi number: FoF02367, Fig. 153 Etymology: The specific epithet ‘‘metroxyli’’ refers to host of which the taxon was collected. Holotype: MFLU 15-0704 Saprobic on dead Metroxylon sagu (Arecaceae). Sexual morph Ascomata 87–95 lm high 9 158–167 lm wide, solitary, immersed, uniloculate, globose to subglobose, surrounded by a dark brown layer of peridium cells. 123 Peridium 9–17 lm wide, composed of outer layers of black, thick-walled cells of textura angularis, inner layers hyaline and thin-walled. Asci 93–100 9 3–4 lm ( x = 97 9 4 lm, n = 10), 8-spored, unitunicate, cylindrical, long pedicellate. Ascospores 9–11 9 1.7–2.6 lm ( x = 12.2 9 2.2 lm, n = 10), uni-seriate, oblong to cylindrical, unicellular, hyaline, with two guttules, thinwalled, smooth-walled, with a distinct mucilaginous sheath. Asexual morph Undetermined. Culture characteristics: Ascospores germinating after 24 h on MEA. Colony on MEA reaching 1.5–2.3 cm diam. after 13 weeks at 25 °C. Center of the colony grey, slightly wrinkled, margin uneven; reddish-brown pigment diffusing into the agar. Material examined: THAILAND, Krabi Province, on dead Metroxylon sagu Rottb. (Arecaceae), 8 December 2014, S. Konta (MFLU 15-0704, holotype, HKAS 95031, isotype), ex-type living culture, MFLUCC 15-0250. Fungal Diversity (2016) 80:1–270 225 Basidiomycota Agaricales Underw. Clavariaceae Chevall. Clavariaceae is a family of the order Agaricales and is characterised by erect, simple or branched, white, pale or brightly coloured basidiomata. The hyphal system is monoor dimitic, with inflated hyphae or not, clamped or not. The cystidia are rare and the basidiospores are variable in shape, usually globose to ellipsoid, with smooth to ornamented walls, hyaline to pale yellow, and non-amyloid (Donk 1964). The family has seven genera and about 120 species (Kirk et al. 2008), widely distributed in the world (Corner 1950, 1970). They are reported as saprobes, ectomycorrhizal, and possibly biotrophs (Birkebak et al. 2013). Clavulinopsis Overeem A genus among the clavarioid fungi that comprises 33 species distributed worldwide (Kirk et al. 2008). The species are usually terrestrial, rarely lignicolous, solitary or gregarious, and characterized by white, yellow, orange or red, simple or branched basidiomata; hyaline or slightly yellow, globose or ellipsoid basidiospores, usually smooth, but echinulate in some species, and tramal hyphae and basidia with clamp connections (Corner 1950, 1970; Petersen 1978). However, the circumscriptions of Clavulinopsis sensu Corner (1950, 1970) and Petersen (1978) were not supported by Birkebak et al. (2013). The phylogenetic tree is presented in Fig. 154. Clavulinopsis aurantiaca Araujo-Neta, Silva & Gibertoni, sp. nov. MycoBank MB 816939; Facesoffungi number: FoF02463, Fig. 155 Etymology: aurantiaca (Latin) = orange-coloured, referring to the colour of the basidiomata. Holotype: URM 84216. Basidiomata simple, 1.5–3 cm, solitary or in small groups, slender, with sharp tips, acute, robust, orange (48), with whitish base (78 W) when fresh. Basidiospores globose, rarely subglobose, 5–6 9 5–6 lm, Q = 1.01, hyaline, guttulate or not, thinwalled, with short apiculus (0.2–0.5 lm), IKI-. Hyphal system monomitic, tramal hyphae parallel, hyaline, abundantly clamped, 2–3 lm wide, inflated up to 10 lm, also clamped, subhymenium tightly interwoven. Cystidia of any kind absent. Basidia elongate-clavate, 35–37 9 5–9 lm, hyaline, guttulate, with 4 (rarely 5) stout sterigmata, 5–6 lm long, clamped at the base. Material examined: BRAZIL, Pernambuco: Tamandaré, Reserva Biologica de Saltinho, August 2011, L.S. AraujoNeta (URM 84216, holotypus; isotype in O); BRAZIL, Pernambuco, Tamandaré, Reserva Biológica de Saltinho, February 2011, L.S. Araujo-Neta (URM 84212), July 2011, Fig. 163 Rhodocybe griseoaurantia (CAL 1324, holotype). A Basidiome in the field. Scale bar 10 mm (Photo by K.N. Anil Raj) L.S. Araujo-Neta (URM 84212), March 2012, L.S. AraujoNeta (URM 84272), June 2013, L.S. Araujo-Neta & V.R. Coimbra 44NA (URM 85691), L.S. Araujo-Neta & V.R. Coimbra 45NA (URM 85692), L.S. Araujo-Neta & V.R. Coimbra 46NA (URM 85693). Notes: This species was found as several solitary basidiomata scattered on soil and is characterized by the slender, flexible, and orange basidiomata when fresh, brittle when dry, and hyaline, mostly globose basidiospores. The specimens of C. aurantiaca were placed in a separated, well supported clade (100/1.0) more closely related, but with little support (63/0.58), to Ramariopsis aurantioolivacea R.H. Petersen, C. fusiformis (Sowerby) Corner, C. helvola (Pers.) Corner and C. laeticolor (Berk. & M.A. Curtis) R.H. Petersen (Fig. 154). These species have simple, orange basidiomata, but R. aurantio-olivacea has narrower, subglobose to ovate basidiospores (5.4–6.5 9 3.6–5 lm) and so far is restricted to New Zealand (Petersen 1988). Clavulinopsis fusiformis, C. helvola and C. laeticolor have been reported in the Neotropics (Petersen 1968; Corner 1950, 1970; Furtado et al. 2016), but C. helvola differs from C. aurantiaca by the angular basidiospores. Clavulinopsis fusiformis (Sowerby) Corner has, on average, larger, globose, subglobose to broadly ovate basidiospores [4.8–7.5(– 9.2) 9 4.5–7.2(–9.2) lm], while C. laeticolor (Berk. & 123 226 Fungal Diversity (2016) 80:1–270 Fig. 164 Rhodocybe griseoaurantia (CAL 1324, holotype). a Basidiospores. b Basidia. c Cheilocystidia. d Pileipellis. e Stipitipellis. Scale bar a–e = 10 lm (Photos by K.N. Anil Raj) M.A. Curtis) R.H. Petersen has broadly ellipsoid to subglobose basidiospores (4.3–7.5 9 3.5–6 lm). Other species of Clavulinopsis with yellow to orange, simple basidiomata have been reported from the Neotropics. Clavulinopsis aurantiocinnabarina (Schwein.) Corner, however, has hyaline to pale yellow, subglobose basidiospores (5–7.1 9 4–7.1 lm), while C. calocera (G.W. Martin) Corner has longer, cylindrical to suballantoid basidiospores (8.5–10 9 4–5 lm). Clavulinopsis amoena (Zoll. & Moritzi) Corner presents pale yellow basidiomata and subglobose basidiospores (4–7 9 4–6.5 lm) (Petersen 1968; Corner 1950, 1970). Ramariopsis (Donk) Corner A genus among the clavarioid fungi that comprises 44 species distributed worldwide. The species have variously coloured, branched or rarely simple basidiomata; hyaline, ellipsoid or subglobose, finely verruculose or echinulate basidiospores, and tramal hyphae and basidia with clamp connections (Corner 1950, 1970). The species are terrestrial, rarely lignicolous, solitary or gregarious (Corner 1950, 1970; Petersen 1978). Garcı́a-Sandoval et al. (2005) considered Ramariopsis sensu Corner (1950, 1970) as a monophyletic group, limiting their species to those with 123 branched basidiomata, echinulate basidiospores and cyanophilous spore ornamentation derived from the tunica. According to Birkebak et al. (2013), the circumscriptions of Ramariopsis sensu Corner (1950, 1970) and Petersen (1978) were not supported. A phylogenetic tree is presented in Fig. 154. Ramariopsis atlantica Araujo-Neta, Silva & Gibertoni, sp. nov. MycoBank MB816940; Facesoffungi number: FoF02464, Fig. 156 Etymology: referring to the Atlantic Rain Forest. Holotype: URM 84210. Basidiomata branched, 5–3.2 cm, with dichotomous, erect and cylindrical branches, solitary to gregarious, white (2B) when fresh, beige to straw when dry (52B, 50S). Basidiospores broadly ellipsoid, 4 9 3 lm, Q = 1.33, hyaline to pale yellow, guttulate or not, echinulate, spines 0.2–0.5 lm, IKI-. Hyphal system monomitic, tramal hyphae hyaline to yellow in mass, 2–3 lm, conspicuously clamped, some inflated up to 10 lm, hardly clamped; subhymenium loose. Cystidia of any kind absent, but hyphal ends resembling gloeocystidia occasionally present. Hymenium collapsed, basidia not seen. Fungal Diversity (2016) 80:1–270 Material examined: BRAZIL, Pernambuco, Tamandaré, Reserva Biológica de Saltinho, July 2011, L.S. AraujoNeta (URM 84210 holotype, isotype in O). BRAZIL, Pernambuco, Tamandaré, Reserva Biologica de Saltinho, July 2011, L.S. Araujo-Neta (URM 84213). A culture was obtained from URM 84213 and deposited in the URM Culture Collection (URM 6985). Notes: Ramariopsis atlantica was collected as several basidiomata scattered on soil and is characterized by branched basidiomata, with dichotomous, cylindrical branches, inflated hyphae and echinulate, ellipsoid to subglobose basidiospores. Ramariopsis kunzei (Fr.) Corner has similar basidiomata (branched, whitish), but smaller basidiospores (3–4.5 9 2.5–3 lm, minutely echinulate, verruculose or asperulate). Ramariopsis atlantica was placed in a separated, well supported subclade (100/1.0), closely related with good support (81/0.97) to R. aff. kunzei and R. tenuiramosa Corner (Fig. 154). The new species can be distinguished from R. tenuiramosa by the larger basidiospores (3.5–4.5 9 3–3.5 lm in R. tenuiramosa) (Corner 1950, 1970). Additionally, R. atlantica was distantly related to all specimens identified as R. kunzei from the USA and the UK. Fig. 165 Phylogenetic tree generated with RAxML (GRT GAMMA) based on ITS sequence data aligned with MAFFT. Bootstrap values higher than 50 % are shown. The sequence of the holotype strain is in bold. The tree is rooted with Nidula niveotomentosa SWFC3000. Species names are followed by strain numbers. Ex-type strains are highlighted in bold and new isolates are in blue 227 Cortinariaceae R. Heim ex Pouzar The limits of the family Cortinariaceae remain unknown. Currently, this family harbors about 12 genera, 2104 species (Kirk et al. 2008). The majority of the species in this family are in the genus Cortinarius. Many genera formerly placed in the Cortinariaceae, e.g., Phaeocollybia, Hebeloma, and Galerina, and some others have been moved to other families in Agaricales. On the other hand, the sequestrate genera, Thaxterogaster, Quadrispora, Protoglossum and Hymenogaster as well as Cuphocybe, Rapacea and species of Rozites, once thought to be genera within the Cortinariaceae, are currently included in the genus Cortinarius (Peintner et al. 2001, 2002). The basidiocarps range from agaricoid to sequestrate, and many have poorly to well-developed veils. The basidiospores are typically ornamented and cinnamon brown in deposit. Cortinarius (Pers.) Gray Cortinarius is the largest genus of Agaricales with a cosmopolitan distribution and over 2000 described species (Kirk et al. 2008). The species are important ectomycorrhizal fungi and are associated with trees and shrubs, belonging to the families Fagaceae, Salicaceae, Caesalpiniaceae, Cistaceae, Dipterocarpaceae, Myrtaceae, Rhamnaceae, Rosacea and Pinaceae, as well as some herbaceous plants in the Cyperaceae and Polygonaceae. Revealing the true diversity of species using only morphological and ecological characteristics has proven to be a difficult if not an impossible task. The use of DNA sequence data has made it possible to elucidate phylogenetic relationships within the genus, to show patterns of speciation, and to help define new, convergent and cryptic species (Fig. 157). Cortinarius sect. Fulvescentes Melot and Cortinarius sect. Laeti Melot were initially included in subgenus Telamonia (Brandrud et al. 1998), but recent phylogenetic analyses suggest that they form a distinct lineage in genus Cortinarius (Peintner et al. 2004; Harrower et al. 2011; Garnica et al. 2016). These two sections were traditionally distinguished from one another by the colour of the universal veil; the species of the section Fulvescentes have pinkish to vinaceous universal veil and species of the section Laeti yellow to ochraceous veil, but sometimes the veil is very indistinct and difficult to observe. The phylogenetic analysis of Harrower et al. (2011) and Garnica et al. (2016), however, suggested that the division based on the veil colour is at least partly unnatural, and it seems a likely hypothesis based on our analysis too. Additional studies are needed to reveal the natural divisions within the clade Fulvescentes/Laeti. Characteristics of the species in these two sections are mat (dull and flat without a shine), hygrophanous pileus, silky-shiny fibrillose stipe, uniformly yellowish-brown context in the stipe, and indistinct smell. 123 228 Fungal Diversity (2016) 80:1–270 Fig. 166 Cyathus pyristriatus (holotype). a Basidiomata. b Basidiomata in top view showing peridioles. c Peridioles and plications on the inner surface of peridium by longitudinal section. d, e Basidiomata in side view showing fluffy hairs. f Basidiospores. g Purse hyphae showing clamp-connections. Scale bars a = 3 mm; b–d = 1 lm; f, g = 10 lm Below we propose a neotype for C. fulvescens Fr. and describe four new species belonging to this clade. hyphae 4–13 lm wide, smooth to encrusted. Hypoderm distinct, elements up to 30 9 65 lm. Trama hyphae 4–13 lm wide, encrusted. ITS sequence (holotype) distinct from the other known members of the clade Fulvescentes/ Laeti, and differs from them in the ITS region by more than 14 substitutions and indel positions. Ecology and distribution: In mesic to dry coniferous forests with Picea and/or Pinus and Tsuga. Known from eastern North America and Europe. Material examined: CANADA, Québec, Montebello, mixed forest, under Tsuga canadensis, 27 September 2010, leg. K. Liimatainen & T. Niskanen 10-153, 10-169 (H). FINLAND, Varsinais-Suomi, Lohja, dry Pinus sylvestris heath forest on sandy soil with lime dust effect, 19 September 2004, leg. I. Kytövuori & T. Niskanen 04-866/ H6030077 (H). Vihti, Sipilänmäki, Lintumäki, mesic Picea-dominated forest, 7 October 2001, leg. H. Tuovila & I. Kytövuori (H). Uusimaa, Kirkkonummi, submesic Picea abies dominated forest with some Betula and Populus tremula, in places herb-rich in others more Pinus dominated, 23 September 2004, leg. T. Niskanen 04-935, H6031281 (neotype, H; isoneotype, K). Uusimaa, Sipoo, Paippinen, dryish to mesic, mossy coniferous forest (Picea abies, Pinus sylvestris) with some Betula, 14 September Cortinarius fulvescens Fr., Epicr. syst. mycol. (Upsaliae): 311 (1838) Facesoffungi number: FoF02465, Fig. 158 Neotype: T. Niskanen 04-935 (H), designated here. Pileus 25–50 mm diam., conical to hemisphaerical, later low convex with an umbo, narrowly pellucid-striate, surface mat, red brown to vinaceous red brown, hygrophanous. Lamellae adnexed, subdistant, moderately broad, moderately thick, at first light medium brown, becoming cinnamon brown, edges remaining pale for some time, then concolorous. Stipe 50–120 mm long, apex 3–9 mm thick, equal, whitish silky-fibrillose. Universal veil pale pink, very sparse, forming incomplete girdles on the stipe. Basal mycelium white. Context in pileus dark red brown, in stipe medium yellow brown. Odor indistinct. Basidia 4-spored, 8–9 9 27–34 lm, clavate. Basidiospores 7.9–9.5 9 4.5–5.2 lm, Q = 1.64–1.90, narrowly amygdaloid to narrowly amygdaloid-ellipsoid, moderately, often somewhat sharply verrucose, slightly to moderately dextrinoid. Lamella trama hyphae moderately to fairly strongly encrusted in MLZ. Pileipellis duplex (in MLZ): Epicutis 123 Fungal Diversity (2016) 80:1–270 229 Fig. 167 Phylogeny of Polyporus and related genera generated by maximum likelihood based on combined ITS ? LSU sequence data. Branches are labeled with maximum likelihood bootstrap proportions (the former values) higher than 50 %, maximum parsimony bootstrap proportions (the middle values) higher than 50 % and Bayesian posterior probabilities (the latter values) more than 0.95 2004, leg. K. Liimatainen & T. Niskanen 04-792, H6029527 (H). Etelä-Häme, Orivesi, Päilahti, Ojanperä, mesic Picea abies forest with some Pinus sylvestris and deciduous bushes, 26 September 1995, leg. I. Kytövuori 95-1547 (H). Notes: Cortinarius fulvescens is a medium-sized species and a typical member of traditional section Fulvescentes with mat, red-brown to vinaceous red-brown pileus, whitish silky-fibrillose stipe, pale pink universal veil, and uniformly yellow brown context in the stipe. It can be 123 230 Fig. 168 Basidiome of Polyporus mangshanensis (holotype). Scale bar 1 cm distinguished from most other species with pinkish to vinaceous veil by somewhat robuster appearance and the narrowly amygdaloid, somewhat more strongly verrusoce spores. The species was described by Fries (1838) and the short description fits well to our species, except the pale pinkish universal veil is not mentioned. This characteristics, however, is easy to miss since the veil is very sparse and sometimes invisible as e.g. in the basidiomata in the middle in Fig. 158. Fries also has two unpublished plates of C. fulvescens (S0313 and S0314, available at the Krypto-S database in the Swedish Museum of Natural History, http:// andor.nrm.se/fmi/xsl/kryptos/kbo/publFindspecies.xsl?-view &-db=kbo_svampregister&-token.languagecode=en-GB), painted after the description of the species. They both represent typical species of traditional section Fulvescentes: the stature is slender, the colour of the pileus is brown to reddish-brown, flesh is brownish-yellow and they do not have any strong veil bands in the stipe. However, the two plates may represent two different species. The basidiomata presented in S0313 illustrate a species common to coniferous heath forests of Scandinavia. It also fits with the current interpretation of the species (Niskanen and Kytövuori 2012). We therefore propose specimen 04-935 as a neotype of the species. Cortinarius fulvescentoideus Kytöv., Niskanen & Liimat., sp. nov. Index Fungorum number: IF552368; Facesoffungi number: FoF02466, Fig. 158 Etymology: The name refers to affinity to C. fulvescens. Holotype: K. Liimatainen & T. Niskanen 03-1634 (H). Pileus 10–25 mm diam., conical to somewhat hemisphaerical, umbonate, later low convex to plane with an umbo or not, up to 1/2 pellucid-striate, surface mat, warm red-brown, hygrophanous. Lamellae adnexed, subdistant, 123 Fungal Diversity (2016) 80:1–270 moderately broad to broad, moderately thick, at first light medium brown, becoming rich brown, edges remaining pale for some time, then concolorous. Stipe 40–100 mm long, apex 3–5 mm thick, equal, whitish silky-fibrillose. Universal veil pale pink, very sparse, forming incomplete girdles on the stipe. Basal mycelium white. Context in pileus dark red-brown, in stipe medium yellow-brown. Odour indistinct. Basidia 4-spored, 8–9 9 30–35 lm, clavate. Basidiospores 8.2–9.5 9 (4.5–)5–5.4 lm, Q = 1.64–1.76, ellipsoid to narrowly ellipsoid, finely to moderately verrucose, slightly to somewhat moderately dextrinoid. Lamella trama hyphae moderately to strongly encrusted in MLZ. Pileipellis duplex (in MLZ): Epicutis hyphae 5–12 lm wide, smooth to encrusted. Hypoderm distinct, elements up to 28 9 60 lm. Trama hyphae 5–13 lm wide, encrusted. ITS sequence (holotype) differs from the sister species C. fulvescens and C. tenuifulvescens more than 7 substitutions and indel positions. Ecology and distribution: In mesic to moist coniferous forests with Picea and Tsuga, often in Sphagnum. Widely distributed and found from Japan, western North America and Europe. Material examined: FINLAND, Etelä-Häme, Orivesi, Päilahti, mesic to moist Picea abies dominated forest with some Pinus sylvestris and hardwood bushes, 26 September 1995, leg. I. Kytövuori 95-1550 (H). SLOVAKIA, Liptovská kotlina basin, Važec, Važecké lúky, Picea abies forest, 29 September 2003, leg. K. Liimatainen & T. Niskanen 03-1634, H7018165 (holotype, H; isotype, K), 30 September 2003 leg. K. Liimatainen & T. Niskanen 03-1675, H7018157 (H). Additional specimens: CANADA, British Columbia, cloneSWUBC478, root tip, GenBank no. DQ481834. JAPAN, Yamanashi, Mt. Fuji, Tsuga diversifolia, June 2011, spYM454 root tip, GenBank no. AB848431. U.S.A., Alaska, Bonanza Creek LTER, Site TKN0109, Picea mariana, 2004, clone3199N24, Notes: Cortinarius fulvescentoideus looks like a slender C. fulvescens and forms a well-supported clade with C. fulvescens and C. tenuifulvescens but strains from two species are partitioned in two highly supported monophyletic subclades that support their delineation (Fig. 157). It is morphologically very similar to C. tenuifulvescens and the two species can easily be misidentified. Our data also indicates that they may have somewhat different ecologies; the collections of C. fulvescentoideus are made from mesic to moist coniferous forests, whereas C. tenuifulvescens prefers sandy, well-drained soils, at least in northern Europe. From the other members of the section Fulvescentes C. fulvescentoideus can most easily be distinguished by the spores. The spores of C. fulvescens are narrowly amygdaloid whereas the spores of C. bulliardioides, C. pseudobulliardioides and C. subfloccopus are broader, [5.5 lm Fungal Diversity (2016) 80:1–270 Fig. 169 Polyporus mangshanensis (holotype). a Basidiospores. b Basidia and basidioles. c Hyphae from context. c1 Generative hyphae, c2 Skeleto-binding hyphae. d Hyphae from cuticle. d1 Contextual cuticle hyphae. d2 Stipe cuticle hyphae. e Hyphae from 231 stipe. e1 Generative hyphae. e2 Skeleto-binding hyphae. f Hyphae from trama. f1 Generative hyphae. f2 Skeleto-binding hyphae. Scale bars a–f = 10 lm 123 232 wide. The spores of C. badiovinaceus are small, ovoidsubglobose. Cortinarius nymphatus Kytöv., Niskanen, Liimat. & Bojantchev, sp. nov. Index Fungorum number: IF551705; Facesoffungi number: FoF02467, Fig. 158 Etymology: Name based on a Greek word nymphe, meaning fairy, since the species is small and elegant Holotype: I. Kytövuori 95-1549 (H) Pileus 10–30 mm diam., at first conical to hemisphaerical, later low convex to almost plane with an umbo, surface mat, brown to dark red-brown, paler towards the margin, hygrophanous. Lamellae adnexed, subdistant, moderately broad, moderately thick, at first light brown, becoming medium brown, edges remaining pale for some time, then concolorous. Stipe 40–60 mm long, apex 2–4 mm thick, equal, whitish silky-fibrillose. Universal veil yellow to ochraceous, forming incomplete girdles on the stipe. Basal mycelium white. Context in pileus dark redbrown, in stipe medium yellow-brown. Odour indistinct. Basidia 4-spored, 6.5–8 9 25–30 lm, clavate. Basidiospores 6.8–8.2 9 4.3–4.8 lm, Q = 1.56–1.76, amygdaloid, finely to moderately, sharply verrucose, slightly to moderately dextrinoid. Lamella trama hyphae moderately encrusted in MLZ. Pileipellis duplex (in MLZ): Epicutis hyphae 4.5–10 lm wide, encrusted. Hypoderm distinct, elements up to 30 9 60 lm. Trama hyphae 5–13 lm wide, somewhat encrusted. ITS sequence (holotype) distinct from the other known members of the clade Fulvescentes/Laeti, and differs from them in the ITS region by more than 7 substitutions and indel positions. Ecology and distribution: In dry to mesic coniferous forests (Pinus, Picea). Known from Europe, Canada, British Columbia. Material examined: CANADA, British Columbia, near Squamish, temperate coniferous rainforest (Picea sitchensis, Pseudotsuga menziesii), 19 October 2009, leg. D. Bojantchev DBB21430 (UC). FINLAND, Etelä-Häme, Orivesi, Päilahti, mesic to moist Picea abies forest with some Pinus sylvestris and hardwood bushes, 26 September 1995, leg. I. Kytövuori 95-1549 (holotype, H; isotype, K). Kainuu, Suomussalmi, W of Hossa, dry Pinus sylvestris heath forest on sandy soil, 16 September 2002, leg. I. Kytövuori, K. Liimatainen & T. Niskanen 02-565 (H). Notes: Cortinarius nymphatus looks like a small Fulvescentes species, but with a yellow-ochraceous veil. Also based on our phylogenetic analysis it seems to belong to section Laeti s. str. with the yellow-veiled type species, C. laetus, of section Laeti. With the combination of small fruiting bodies and small spores it can be distinguished from the other yellow veiled species of the clade Fulvescentes/Laeti. 123 Fungal Diversity (2016) 80:1–270 Cortinarius pseudobulliardioides Kytöv., Niskanen, Liimat. & Ammirati, sp. nov. Index Fungorum number: IF551704; Facesoffungi number: FoF02468, Fig. 158 Etymology: The name refers to affinity and similar appearance to C. bulliardioides. Holotype: J.F. Ammirati & K. Liimatainen 11-452 (H). Pileus 25–45 mm diam., at first conical, later low conical to almost plane with an umbo surface mat, dark reddish-brown, somewhat paler towards the margin, hygrophanous. Lamellae adnexed-emarginate, subdistant, moderately broad, moderately thick, at first light medium brown, becoming medium brown, edges remaining pale for some time, then concolor. Stipe 40–90 mm long, apex 4–7 mm thick, equal, whitish silky-fibrillose. Universal veil pink, forming broad, incomplete girdles on the stipe. Basal mycelium white. Context in pileus dark red brown, in stipe medium to light yellow brown. Odor indistinct. Basidia 4-spored, 9–11 9 35–40 lm, clavate. Basidiospores 8.4–10 9 5.5–6.6 lm, Q = 1.38–1.54, ellipsoid, finely verrucose, moderately dextrinoid. Lamella trama hyphae moderately to strongly encrusted in MLZ. Pileipellis duplex (in MLZ): Epicutis hyphae 6–14 lm wide, encrusted. Hypoderm distinct, elements up to 25 9 40 lm. Trama hyphae 5–12 lm wide, encrusted. ITS sequence (holotype) distinct from the other known members of the clade Fulvescentes/Laeti, and differs from them in the ITS region by more than 7 substitutions and indel positions. Ecology and distribution: In coniferous forests (Abies, Picea, Pinus, Tsuga). Widely distributed and known from western North America and Europe. Material examined: FINLAND, Koillismaa, Kuusamo, Oulanka National Park, forests close to Kiutaköngäs, dry Pinus sylvestris heath forest with some Picea and Betula on sandy and calcareous soil, 18 September 2002, leg. I. Kytövuori, K. Liimatainen & T. Niskanen 02-689 (H). U.S.A, Washington, Kittitas County, Table Mountain, coniferous forest (Abies, Picea, Pinus, Larix), 9 October 2011, leg. J.F. Ammirati & K. Liimatainen 11-452 (holotype, H; isotype, K). Additional specimens: CANADA, British Columbia, Tsuga heterophylla, cloneSLUBC12. Notes: Cortinarius pseudobulliardioides is a rather large species in clade Fulvescentes/Laeti, in appearance similar to sister species C. subfloccopus and somewhat more distantly related C. bulliardioides. Cortinarius subfloccopus, however, usually has vinaceous red universal veil and C. bulliardioides has broader spores, [6 lm wide. Cortinarius tenuifulvescens Kytöv., Niskanen & Liimat., sp. nov. Index Fungorum number: IF551703; Facesoffungi number: FoF02469, Fig. 158 Fungal Diversity (2016) 80:1–270 233 Fig. 170 Phylogram generated from maximum likelihood method based on ITS-rDNA sequences from MEGA6 under Kimura 2-parameter model (Kimura 1980). The tree with the highest log likelihood (-1342.1525) is shown. Onethousand bootstrap replicates were analyzed to obtain the nodal support values. The novel species having GenBank Accession Number KX234820 (ITS-rDNA) is shown in blue. The R. brevipes, R. chloroides, R. emetica are considered as the out groups Etymology: The species looks like a slender C. fulvescens. Holotypus: K. Liimatainen & T. Niskanen 04-572, H6031278 (H) Pileus 10–30 mm diam., conical to somewhat hemisphaerical, umbonate, later low convex to plane with an umbo or not, up to 1/3 pellucid-striate, surface mat, warm redbrown, hygrophanous. Lamellae adnexed, subdistant, moderately broad, moderately thick, at first light brown, becoming medium brown, edges remaining pale for some time, then concolorous. Stipe 40–110 mm long, apex 2–4 mm thick, equal, whitish silky-fibrillose. Universal veil pale pink, very sparse, forming incomplete girdles on the stipe. Basal mycelium white. Context in pileus dark red-brown, in stipe medium yellow-brown. Odour indistinct. Basidia 4-spored, 8–9 9 27–35 lm, clavate. Basidiospores 7.5–9.3 9 4.8–5.4 lm, Q = 1.48–1.70, ellipsoid to narrowly ellipsoid, finely to fairly finely, densely, evenly verrucose, slightly to somewhat 123 234 Fungal Diversity (2016) 80:1–270 Fig. 171 Russula indoalba (holotype). a–c Fresh basidiomata in the field and base camp. d Transverse section through lamellae showing pleuromacrocystidia. e–g Radial section through pileipellis showing elements in chains. h SEM of basidiospores. Scale bars a = 100 mm, d, e = 50 lm, f, g = 10 lm, h = 2 lm moderately dextrinoid. Lamella trama hyphae moderately to strongly encrusted in MLZ. Pileipellis duplex (in MLZ): Epicutis hyphae 4.5–13 lm wide, smooth to encrusted. Hypoderm distinct, elements up to 30 9 70 lm. Trama hyphae 5–13 lm wide, encrusted. ITS sequence (holotype) distinct from the other known members of the clade Fulvescentes/ Laeti, and differs from them in the ITS region by more than 7 substitutions and indel positions. 123 Fungal Diversity (2016) 80:1–270 235 Fig. 172 Russula indoalba (holotype). a Fresh and/or dissected basidiomata. b Radial section through pileipellis. c Basidiospores. d Pleurocystidia. e Cheilocystidia. f Basidia. Scale bars a = 10 mm; b– f = 10 lm Ecology and distribution: In dry to mesic coniferous forests, in northern Europe often on sandy soil, one collection from spruce-hardwood swamp. Widely distributed and found from western and eastern North America and Europe. Material examined: CANADA, Newfoundland, Avalon Peninsula, Butter Pot Provincial Park, mesic to damp Picea dominated forest with some Abies, Larix and Betula, 25 September 2007, leg. K. Liimatainen & T. Niskanen 07-292, H7000956 (H). FINLAND, Perä-Pohjanmaa, Rovaniemi, Pisavaara nature reserve area, 31 August 2004, leg. K. Liimatainen & T. Niskanen 04-572, H6031278 (holotype, H; isotype, K). SWEDEN, Jämtland, Berg, dry Pinus sylvestris heath forest with some Picea and Betula on sandy soil, leg. I. Kytövuori, K. Liimatainen & T. Niskanen 03-907, H7018168 (H). Ångermanland, Strömsund, Bodum, at a small sand pit, Picea-hardwood swamp, leg. I. Kytövuori 97-500b (H). Additional specimens: CANADA, British Columbia, Mt. Washington, Trail to Rossiter Lake, in mossy detritus, 13 Sep 2001, leg. O. Ceska OC43, F17115 (UBC) Notes: See discussion under C. fulvescentoideus. 123 236 Fungal Diversity (2016) 80:1–270 Fig. 173 Phylogram generated from maximum likelihood method based on ITS-rDNA sequences: The evolutionary history was inferred by using the maximum Likelihood method based on the Kimura 2-parameter model (Kimura 1980). The tree with the highest log likelihood (-2215.6038) is shown. One-thousand bootstrap replicates were analyzed to obtain the nodal support values. The novel species having GenBank Accession Number KX234819 (ITS-r-DNA) is shown in blue. The R. emetica and R. nana were considered as the out group taxa. Evolutionary analyses were conducted in MEGA6 (Tamura et al. 2013) Entolomataceae Kotl. & Pouzar Entolomataceae was introduced by Kotlaba and Pouzar (1972) and typified by Entoloma, is highly variable in terms of sporocarp morphology and micromorphology (Noordeloos 2004). To date, this family harbors four genera, 1071 species (Kirk et al. 2008). namely Rhodocybe, Clitopilopsis, Rhodophana and Clitocella. The amended Rhodocybe now incorporates only clampless species (Kluting et al. 2014). In the course of our studies on the Entolomataceae of Kerala State, India, we came across three species of Rhodocybe, which were found to be new to science. They are described here based on both morphology and molecular phylogeny. Maximum likelihood analysis placed the three new species in Rhodocybe clade with significant (76 %) ML bootstrap support (Fig. 159) Rhodocybe Maire The genus Rhodocybe is characterised by pink, vinaceous white or grey spore print and inamyloid, cyanophilic basidiospores that are angular in polar view and with an undulate-pustulate ornamentation (Baroni 1981; Singer 1986). Based on a molecular phylogenetic study, Kluting et al. (2014) split Rhodocybe sensu lato into four genera, 123 Rhodocybe indica K.N.A. Raj & Manim., sp. nov. MycoBank MB 816841; Facesoffungi number: FoF02179, Fig. 160 Fungal Diversity (2016) 80:1–270 237 Fig. 174 Russula pseudoamoenicolor (holotype). a, b Fresh basidiomata. c–e Transverse section through lamellae showing pleuromacrocystidia. f Radial section through pileipellis. g–j Elements of pileipellis. k Basidiospores. l SEM micrograph of basidiospores. Scale bars a = 100 mm; c, d = 50 lm; e, g–k = 10 lm; f = 100 lm; l = 2 lm Etymology: The specific epithet refers to India, the country where this species was first observed. Holotype: CAL 1323. Basidiocarp small, mycenoid. Pileus 15 mm diam., broadly convex with a small umbo; surface brown (6E7/ OAC599) on and around the umbo, brownish-yellow 123 238 Fungal Diversity (2016) 80:1–270 Fig. 175 Russula pseudoamoenicolor (holotype). a Fresh and/or dissected basidiomata. b Radial section through pileipellis. c Basidiospores. d Pleurocystidia. e Basidia. f Cheilocystidia. Scale bars a = 10 mm; b–f = 10 lm (5C7/OAC790) towards the margin, weakly hygrophanous and becoming slightly paler, faintly pellucid-striate towards the margin, glabrous, somewhat tacky; margin slightly incurved and somewhat wavy. Lamellae narrowly adnate to adnate, subventricose, close, greyish-orange (6B3/OAC695), up to 3 mm wide, with lamellulae of 3–7 lengths; edge crenulate, concolourous with the sides. Stipe 30 9 2 mm, central, equal, slightly flexuous, cartilaginous, solid; surface brownish-yellow (5C7/OAC790), glabrous to the naked eye, finely pruinose all over under a 123 lens; base with white mycelial cords. Odour and taste not distinctive. Basidiospores 6.5–8 9 5.5–7 (7.27 ± 0.49 9 6.1 ± 0.38) lm, (Q = 1–1.3, Qm = 1.19), subglobose or lacrymoid, undulate-pustulate, with or without a suprahilar depression in profile view, 6 angled in polar view, hyaline, thin-walled. Basidia 18–33 9 7–8 lm, narrowly clavate to clavate, pale yellow, thin-walled, 4-spored; sterigmata up to 5 lm long. Lamella-edge heterogeneous. Cheilocystidia and pleurocystidia present as pseudocystidia. Fungal Diversity (2016) 80:1–270 239 Pseudocystidia 24–40 9 4–9 lm, scattered, fusiform, lanceolate or ventricose-rostrate, with glittering, yellow, granular contents, thin-walled. Lamellar trama subregular; hyphae 4–7 lm wide, hyaline or pale yellow, thin-walled. Subhymenium inconspicuous. Pileus trama subregular; hyphae 5–14 lm wide, pale yellow, thin-walled. Pileipellis an undifferentiated cutis, made up of closely septate and compactly arranged hyphae with widely scattered pileocystidia; hyphae 5–13 lm wide, slightly gelatinised, with a pale brownish-yellow wall pigment and fine hyaline encrustations, thin- to slightly thick-walled. Pileocystidia 21–42 9 3–11 lm, scattered, versiform: nettle hairshaped, narrowly fusiform or flexuous, hyaline or pale yellow, thin-walled. Stipitipellis a cutis, rarely disrupted by flaring out hyphae; hyphae 3–9 lm wide, with a pale brownish-yellow wall pigment, thin-walled. Caulocystidia absent. Clamp connections not observed on any hyphae. Habitat: on a decaying twig, solitary. Specimens examined: INDIA, Kerala, Kollam District, Thenmala, Thenmala Forest, 17 August 2013, K. P. Deepna Latha DKP130 (CAL 1323, holotype). Notes: Rhodocybe indica is well-characterized by its small basidiocarps with a brownish-yellow, umbonate pileus; subglobose or lacrymoid basidiospores; smaller, fusiform, lanceolate or ventricose-rostrate pseudocystidia; a pileipellis with pileocystidia and a stipitipellis lacking encrusted hyphae. Characters such as the centrally stipitate basidiocarps and the presence of pseudocystidia indicate Fig. 176 Phylogram generated from maximum likelihood method based on ITS-rDNA sequences: the evolutionary history was inferred by using the maximum likelihood method based on the Kimura 2-parameter model (Kimura 1980). The tree with the highest log likelihood (-1884.3936) is shown. One-thousand bootstrap replicates were analysed to obtain the nodal support values. The novel species having GenBank Accession Number KX254611 (ITS-r-DNA) is shown in blue. The Lactifluus vellereus was considered as the out group. Evolutionary analyses were conducted in MEGA6 (Tamura et al. 2013) 123 240 Fungal Diversity (2016) 80:1–270 Fig. 177 Lactarius dirkii (holotype). a Fresh basidiomata. b White latex exuding from cut lamellae. c Transverse section through lamellae edge showing cheiloleptocystidia. d, e Radial section through pileipellis showing pileopseudocystidia (arrows). f Radial section through pileipellis. g Terminal elements of pileipellis. h, i SEM micrograph of basidiospores. Scale bars a = 100 mm; c, f = 50 lm; d, e, g = 10 lm; h = 20 lm; i = 2 lm the section Rhodocybe (Baroni 1981). Rhodocybe pruinosistipitata T.J. Baroni et al., a species reported from Pakaraima Mountains of Guyana (Henkel et al. 2010), is somewhat comparable to the present species because of a similar looking pileus, a pruinose stipe, adnate lamellae, somewhat similar sized basidiospores, abundant pseudocystidia and a similar habitat. However, R. pruinosistipitata has a longer stipe, pip-shaped basidiospores, larger, 123 Fungal Diversity (2016) 80:1–270 241 Fig. 178 Lactarius dirkii (holotype). a Fresh or dissected basidiomata. b Radial section through pileipellis showing terminal and subterminal cells. c Basidiospores. d Cheiloleptocystidia. e Pseudocystidia. f Basidia. Scale bars a = 10 mm; b– f = 10 lm broadly ventricose to lageniform pseudocystidia, a pileipellis lacking pileocystidia and a stipitipellis with encrusted hyphae (Pegler 1977, 1986). Rhodocybe retroflexa (Berk. & Broome) Pegler reported from Sri Lanka (Pegler 1977, 1986) has somewhat similar sized basidiocarps with similarly coloured pileus, almost similarly attached lamellae, almost similar sized basidiospores of similar shape, pseudocystidia with granular contents, and an undifferentiated cutis-type pileipellis. However, that species has a depressed pileus, a pseudoparenchymatous subhymenium, and a pileipellis devoid of encrusted hyphae and pileocystidia. Comparison of the RPB2 (687 bp), ITS (412 bp) and nLSU (884 bp) sequence data derived from Rhodocybe indica with the nucleotide sequences of taxa available in GenBank suggests that the sequences of the present species are different. In a megablast search of the GenBank nucleotide database using the RPB2 and ITS sequences, no close hits with zero e-values were obtained. While using the nLSU sequence, the closest hit was Rhodocybe fallax 123 242 (GenBank AF261283; Identities = 853/884 (96 %)). Rhodocybe fallax (Quél.) Singer), belonging to the section Decurrentes (Baroni 1981), and differs from the present species in almost all macro- and micromorphological characters. In the resulting phylogenetic tree after the ML analysis (Fig. 159), Rhodocybe indica, R. collybioides and R. caelata formed a distinct clade with significant support (73 % BS). Within this clade, R. indica is related to R. collybioides with weak support (67 % BS). Rhodocybe luteobrunnea K.N.A. Raj & Manim., sp. nov. MycoBank MB 816842; Facesoffungi number: FoF02180, Figs. 161 and 162 Etymology: The specific epithet refers to the yellowishbrown pileus. Holotype: CAL 1322. Basidiocarps small, mycenoid. Pileus 5–14 mm diam., initially somewhat conico-convex, becoming convex or broadly convex with a small umbo; surface yellowishbrown (5E8/OAC734) on umbo and striations, brownishyellow (5D5, 5D6/OAC743) elsewhere, rather hygrophanous and becoming paler, finely pellucid-striate, glabrous; margin slightly incurved when young, becoming deflexed to almost straight with age, finely wavy. Lamellae sinuate or emarginate with a small decurrent tooth, close, greyish-orange (5B3/OAC574), up to 3 mm wide, with lamellulae of 3 lengths; edge entire to the naked eye, finely torn under a lens, concolourous with the sides. Stipe 7–26 9 1–2 mm, central or slightly eccentric, terete, equal, straight or somewhat flexuous, cartilaginous, solid; surface brownish-orange (5C3/OAC730), glabrous to the naked eye, finely appressed fibrillose all over under a lens, finely pruinose towards the apex; base with white mycelial cords. Odour and taste not distinctive. Basidiospores 5–7 9 3–4.5 (5.67 ± 0.54 9 3.97 ± 0.49) lm, Q = 1.2–1.7, Qm = 1.44, lacrymoid or pipshaped, with or without a suprahilar depression in profile view, up to 8, weak angular facets in polar view, finely undulate-pustulate all over, thin-walled. Basidia 16–21 9 5.5–6.5 lm, clavate, pale yellow, thin-walled, 4-spored; sterigmata up to 4 lm long. Lamella-edge heterogeneous. Cheilocystidia and pleurocystidia present as pseudocystidia. Pseudocystidia 16–30 9 4–6 lm, narrowly fusiform, narrowly utriform or cylindrical with an acute apex, with golden yellow or reddish-yellow contents, thin-walled. Lamellar trama subregular; hyphae 4–9 lm wide, hyaline or pale yellow, thin-walled. Subhymenium inconspicuous. Pileus trama subregular; hyphae 4–7 lm wide, with a pale yellow wall pigment and fine brown spiral encrustations, thin- to slightly thick-walled. Pileipellis an undifferentiated cutis; hyphae 4–7 lm wide, 123 Fungal Diversity (2016) 80:1–270 with a pale brownish-yellow wall pigment and fine brown encrustations, thin- to slightly thick-walled. Stipitipellis a cutis often disrupted by bunches of flaring out hyphae; hyphae 3–7 lm wide, with a pale brownish-yellow wall pigment and fine brown encrustations, thin- to slightly thick-walled. Caulocystidia 10–14 9 2–4 lm, versiform: lageniform, cylindrical with a short rostrate apex or flexuous, with a pale brownish-yellow wall pigment, thinwalled. Clamp connections not observed on any hyphae. Habitat: in small groups, on forest floor, among decaying litter. Material examined: INDIA, Kerala State, Thrissur District, Peechi, Peechi Forest, 26 July 2010, K. N. Anil Raj AR180 (CAL 1322, holotype). Notes: Rhodocybe luteobrunnea is distinguished by its yellowish-brown basidiocarps with a finely pellucid-striate pileus; sinuate or emarginate lamellae; lacrymoid or pipshaped basidiospores; cylindrical to fusiform pseudocystidia and encrusted pileipellis. A combination of characters such as the centrally stipitate basidiocarps and the presence of pseudocystidia with coloured contents (in KOH) lead the present species to the section Rhodocybe. Rhodocybe perplexa T.J. Baroni & Watling from Malaysia (Baroni and Watling 1999) resembles R. luteobrunnea in having a pileus of similar size and shape, somewhat similar sized basidiospores, presence of pseudocystidia, pileipellis with encrusted hyphae and clampless hyphae (Baroni and Watling 1999). However, R. perplexa has differently coloured basidiocarps, adnate lamellae, shorter stipe, subglobose to ellipsoid basidiospores and larger pseudocystidia. Rhodocybe naucoria Singer, an Argentinean species has similar shaped pileus, similar-sized and somewhat similar-shaped basidiospores and presence of pseudocystidia (Baroni 1981). However, that species differs from the present one in having smaller sized and differentlycoloured basidiocarps, differently attached lamellae, larger pseudocystidia and presence of birefringent crystals in the hymenial hyphae. Comparison of RPB2 (648 bp), ITS (675 bp) and nLSU (830 bp) sequence data derived from the present Rhodocybe species with the nucleotide sequences of taxa available in GenBank suggests that the sequences are different. In a BLASTn search using the ITS sequence derived from the present species, no close hits with a zero e-value were obtained. Rhodocybe aureicystidiata is the closest hit while conducting a BLASTn search using both nLSU (GenBank AY380407; Identities = 840/852 (99 %)) and RPB2 (GenBank AY337412; Identities = 604/648 (93 %)) sequences. Rhodocybe aureicystidiata Lennox ex T.J. Baroni, a species belonging to the section Rhodocybe (Baroni 1981), shows some similarity to the present species in having rather similar-coloured basidiocarps, encrusted hyphae in Fungal Diversity (2016) 80:1–270 243 Fig. 179 Phylogenetic tree of Mortierella fluviae EMLYR25716-1 and EMLYR25716-2 and related species before based on maximum likelihood analysis of ITS rDNA sequences. Sequence of Umbelopsis isabellina was used as outgroup. Numbers at the nodes indicate the bootstrap values ([50 %) from 1000 replications. The bar indicates the number of substitutions per position. New taxa are in blue Fig. 180 Phylogenetic tree of Mortierella fluviae EMLYR25716-1 and EMLYR25716-2 and related species based on maximum likelihood analysis of 28S rDNA sequences. Sequence of Umbelopsis isabellina was used as outgroup. Numbers at the nodes indicate the bootstrap values ([50 %) from 1000 replications. The bar indicates the number of substitutions per position. New taxa are in blue the pileipellis and presence of pseudocystidia. However, that species has larger basidiocarps, a depressed pileus which becomes dark red on bruising, inrolled margin, larger and amygdaliform basidiospores, larger and differently shaped pseudocystidia and a different geographical location (Baroni 1981). In the phylogram (Fig. 159) generated from the ML analysis, Rhodocybe luteobrunnea and R. aureicystidiata, R. pruinosostipitata, R. mellea and Rhodocybe sp. formed a distinct clade with significant support (99 % BS). Within this, R. luteobrunnea and R. aureicystidiata formed another subclade also with significant support (90 % BS). Rhodocybe griseoaurantia K.N.A. Raj & Manim., sp. nov. MycoBank MB 816843; Facesoffungi number: FoF02181, Figs. 163 and 164 Etymology: The specific epithet refers to the greyishorange colour of the pileus. Holotype: CAL 1324. Basidiocarp small. Pileus 14 mm diam., convex with a slightly raised centre; surface greyish-orange (6B6/ OAC694) at the centre and pale orange (6A3/OAC695) toward the margin, hygrophanous and soon becoming orange-white (6A2/OAC696) after collection, not striate, finely appressed squamulose on and around the centre and minutely pubescent towards the margins; margin slightly 123 244 incurved, rather wavy. Lamellae adnate to short decurrent, close, pale orange (6A3/ OAC695), up to 3.5 mm wide, occasionally furcate, with lamellulae of 1–4 lengths; edge entire or finely torn, concolourous with the sides. Stipe 31 9 4 mm, central, terete, slightly tapering towards the apex, solid; surface orange-white (6A2/OAC696), finely appressed-fibrillose all over, finely pruinose towards the apex; base slightly swollen, with whitish mycelial cords. Odour and taste not distinctive. Basidiospores 5–7 9 3.5–4.5 (5.85 ± 0.67 9 3.82 ± 0.33) lm, Q = 1.25–2, Qm = 1.53; with 6–7 facets in polar view, ellipsoid in profile with or without a suprahilar depression, weakly undulate-pustulate all over, hyaline, thin-walled. Basidia 19–25 9 5–7 lm, clavate, hyaline, thin-walled, 4-spored; sterigmata up to 3 lm long. Lamella-edge often fertile or occasionally heterogeneous. Cheilocystidia 14–29 9 3–9 lm, infrequent, scattered, often filiform or cylindrical, sometimes flexuous or narrowly fusiform. Pleurocystidia none. Lamellar trama subregular; hyphae 4–5.5 lm wide, hyaline, thin-walled. Subhymenium inconspicuous. Pileus trama subregular; hyphae 3.5–8 lm wide, pale yellow, thin-walled. Pileipellis a cutis often disrupted by scattered or rarely clustered ascending hyphae; hyphae 2.5–6 lm wide, with a pale yellow wall pigment and occasionally with faint, hyaline encrustations, thin- to slightly thick-walled. Stipitipellis a cutis occasionally disrupted by scattered or clustered flaring-out hyphae; hyphae 3.5–6 lm wide, hyaline or pale yellow, thin-walled. Caulocystidia absent. Clamp connections not observed on any hyphae. Habitat: on soil, among moss, solitary. Material examined: INDIA, Kerala State, Wayanad District, Muthanga, Muthanga Wildlife Sanctuary, 6 September 2011, K. N. Anil Raj AR865 (CAL 1324, holotype). Notes: Rhodocybe griseoaurantia is well characterized by its small basidiocarps with a greyish-orange pileus; adnate to short decurrent lamellae; ellipsoid basidiospores and filiform or cylindrical cheilocystidia are the diagnostic features of R. griseoaurantia. Owing to the centrally stipitate basidiocarp, presence of cheilocystidia and the absence of both pseudocystidia and clamp connections, R. griseoaurantia can be placed in the section Rufrobrunnea (Baroni 1981). Rhodocybe alutacea Singer, a North American species (Baroni 1981; Baroni and Horak 1994), is similar to the present species in having similar coloured basidiocarps, a similar looking pileus, narrow lamellae with similar type of attachment, a solid stipe, almost similar-sized basidiospores of similar shape, infrequent, scattered cheilocystidia, pileipellis with encrusted hyphae and non-encrusted hyphae of stipitipellis. However, R. alutacea has larger basidiocarps, an umbilicate pileus, basidiospores with more facets (7–9) in profile view, larger, septate cheilocystidia, presence of 123 Fungal Diversity (2016) 80:1–270 Fig. 181 Mortierella fluviae (holotype). a, d Colony in potato c dextrose agar. b, e Colony in oatmeal agar. c, f Colony in water agar (a–c above view, d–f reverse view) (g–r light microscope; s–u SEM). g–j Young and mature sporangia on sporangiophores and apophysis (i, s) (white arrow). k–m Single and branched sporangiophores. n– r Different shapes of columellae (r, t) (purple arrow) with collarette (green arrow) and sporangial septum (yellow arrow). u Spores. Scale bars g–j, n, p–r = 20 lm, k–m, o = 50 lm, s = 30 lm, t = 10 lm, u = 5 lm caulocystidia and a different habitat and geographical location. Comparison of the RPB2 (681 bp), ITS (733 bp) and nLSU (908 bp) sequence data derived from R. griseoaurantia with the nucleotide sequences of taxa available in GenBank suggests that the present species is different. In the BLASTn search using the RPB2 sequence, the closest hit was Rhodocybe truncata (GenBank EF421019; Identities = 616/659 (93 %)). For ITS sequence, the closest hit was an unidentified Rhodocybe species (Rhodocybe sp. 1 GMB-2014) (GenBank KP012803; Identities = 719/737 (98 %)) from Australia, followed by R. truncata (GenBank EF421110; Identities = 556/644 (86 %)). In the BLASTn search with the nLSU sequence, the closest hits was Rhodocybe gemina (GenBank DQ071715; Identities = 894/ 908 (98 %)). Rhodocybe truncata (Schaeff.) Singer, a European species, differs from the present one in almost all macro- and microscopic characters, although it has cheilocystidia and rather similar-sized (5–6.5 9 4–5 lm) basidiospores (Baroni 1981). Rhodocybe gemina (Paulet) Kuyper & Noordel., another European species belonging to the section Rufrobrunnea, differs from the present species in having larger and differently-coloured basidiocarps, crowded lamellae, a perfumed odour, unpleasant taste, slightly larger and differently shaped basidiospores (5–7 9 4–5.5 lm), and a cutis with transition to a trichoderm type of pileipellis (Noordeloos 1988). In the phylogram (Fig. 159), R. griseoaurantia, R. truncata and R. gemina formed a strongly support clade. This clade received significant support (98 % BS) and within it R. griseoaurantia was found to be phylogenetically distinct from R. truncata and R. gemina. Agaricaceae Chevall. We follow Li et al. (2016) Cyathus Haller The genus Cyathus belongs in the Agaricaceae, despite the unusual form of its basidiomata (also known as peridia), which resemble a bird’s nest and grow on wood or dung. These peridia are typically vase-, trumpet- or urnshaped with dimensions of 4–8 mm wide and 7–18 mm high. The type species, Cyathus striatus (Huds.) Willd. was described from Europe, but the genus has a cosmopolitan distribution and comprises ca. 45 species (Das et al. 2016). Fungal Diversity (2016) 80:1–270 245 123 246 Fungal Diversity (2016) 80:1–270 Fig. 182 Phylogenetic tree of Cunninghamella constructed using the ITS rDNA sequences. Absidia anomala and Halteromyces radiatus were used as outgroup taxa. Sequences are labeled with their database accession numbers. Support values are from Bayesian inference. Sequences obtained in this study are clones annotated in blue Besides the studies of Zhao et al. (Zhao et al. 2006, 2007), the tropical species are less known, and we here describe a new taxon from Thailand that was recently identified as a 123 producer of novel terpene alkaloid antibiotics. A phylogenetic tree in support of the new species is provided in Fig. 165. Fungal Diversity (2016) 80:1–270 Fig. 183 Phylogenetic tree of Cunninghamellaceae constructed using the large subunit (LSU) rDNA sequence data. Mortierella parvispora species was used as outgroup. Sequences are labeled with 247 their database accession numbers. Support values are from Bayesian inference. The sequences obtained in this study are clones annotated in blue 123 248 123 Fungal Diversity (2016) 80:1–270 Fungal Diversity (2016) 80:1–270 b Fig. 184 Cunninghamella gigacellularis (holotype). a Colony sur- face. b Sporophore with branches in pair. c Sporophore with branches in two whorls of 4 and 3. d Sporophore with single and in pairs branches. e, f Sporophore repeatedly branched. g Sporophore branched. h Giant cels. i Sporangiola Cyathus pyristriatus B. Thongbai, C. Richt. & M. Stadler, sp. nov. MycoBank number: MB817167; Facesoffungi number: FoF02385, Fig. 166 Etymology: Named for its production of pyristriatins A and B. Holotype: MFLU15-1416. Saprobic on rotten wood in forest with Fagaceae. Basidiomata clavate to broadly obconic, without stipe, 5.5–7 mm high and 4–6 mm diam., wide at the top, external peridium covered with shaggy or fluffy yellowish-brown or buff hairs with age, surface of inner peridium grey, darkening with age, distinctly plicate. Peridioles 3–3.5 mm in diam. wide, greyish-brown to dark grey covered with minute, greyish to greyish-brown hairs. Basidiospores 14–17 9 8–10 lm (av. = 15.18 9 8.12, Q = 1.5–1.91, Qm = 1.70, n = 40), ellipsoid to broadly ellipsoid, some ovoid, rarely subglobose, hyaline, smooth, thin-walled, 1.5–3 lm thick. Basidia not observed. Clamp connections present in all tissues including the mycelial culture. Material examined: THAILAND, Chiang Mai Province, Mae-Taeng District, near the Mushroom Research Centre (http://www.mushroomresearchcentre.com/), N19°07.2000 E98°44.0440 , 12 August 2014, Thongbai, Richter & Stadler M68 (MFLU 15-1416, holotype), ex-type culture MFLUCC14-0770. Notes: Cyathus pyristriatus closely resembles C. striatus (Huds.) Willd. 1787 in the account of shaggy or fluffy yellowish-brown or buff hairs of peridium, distinctly plicate inner peridium, while peridioles are greyish-brown to dark grey. Based on morphological comparison between C. striatus and C. pyristriatus the latter has smaller basidioma and also smaller ellipsoid basidiospores. However, peridioles are covered with a distinctive minutely greyish to greyish-brown hairs, while peridioles of C. striatus are smooth. Remarkably, C. striatus was reported producing pale to dark pigments from basidiomata, which vary in size (Kuo 2014; Wood and Stevens 2015). An initial BLAST search of the ITS nucleotide sequence from NCBI database (http://www.ncbi.nlm.nih.gov/) gave the closest hit to a Cyathus striatus strain (EU784194) with a maximum similarity of 94.5 % (Brock et al. 2009). The BLAST search of the LSU nucleotide sequence of C. pyristriatus resulted in a high degree similarity of 98.8 % (DQ071742) with C. striatus (Garnica et al. 2007). Figure 165 illustrates the phylogenetic relationships of 249 C. pyristriatus, C. striatus, and other Cyathus species. The ITS based phylogenies clearly depict that our new taxon belongs to the genus Cyathus. A close affinity between C. pyristriatus and C. stercoreus collected from China, is also noted with high support. Cyathus stercoreus, however, differs in having a smooth surface of inner peridium and larger globose to oval basidiospores (Zhao et al. 2008). The ex-type culture of the new species also produces novel terpene alkaloid antibiotics named pyristriatins, which have never been found in Cyathus, despite the fact that this genus has been evaluated intensively for secondary metabolites for several decades (Richter et al. 2016). Polyporales Gäum. Polyporaceae Fr. ex Corda We follow Zhou et al. (2016). Polyporus P. Micheli ex Adans. Polyporus, the type genus of Polyporaceae, is characterized by annual stipitate basidiocarps, a dimitic hyphal system with generative hyphae and skeleto-binding hyphae, hyaline and thin-walled cylindrical basidiospores, and are the cause of white rot (Gilbertson and Ryvarden 1987). Phylogenetic analysis reports Polyporus as polyphyletic (Ko and Jung 2002; Krüger et al. 2006; Sotome et al. 2008, 2011). Recently, based on a multi-locus phylogeny, species of Polyporus, Favolus and Neofavolus were divided into six highly supported clades, i.e. Favolus clade, Melanopus clade, Neofavolus clade, Polyporellus clade, Polyporus clade and Squamosus clade (Zhou et al. 2016). A phylogenetic tree in support of the new species is provided in Fig. 167. Polyporus mangshanensis B.K. Cui, J.L. Zhou & Y.C. Dai, sp. nov. Index Fungorum number: IF552159; Facesoffungi number: FoF02471, Figs. 168 and 169 Etymology: mangshanensis, referring to the locality of the type specimen. Holotype: BJFC 018267. Basidiocarps annual, eccentrically to almost laterally stipitate, solitary, corky when dry; pileus reniform, projecting up to 5.8 cm long, 10.5 cm wide and 2.5 cm thick; pileal surface beige, saffron yellow to yellowish-orange when dry, azonate, with slightly radial stripes, smooth and glabrous; margin sharp and involute upon drying; pore surface brown beige to olive brown when dry; pores angular, 3–5 per mm, occasionally elongated to 1 mm long and 0.5 mm wide; dissepiments thin, entire to lacerate; context buff when dry, up to 1.2 mm thick; tubes concolorous with the pore surface, slightly decurrent on the stipe, up to 1.5 mm long; stipe cylindrical, context of stipe buff when dry, bearing dark brown cuticle, becoming tan 123 250 towards the tuber layer, glabrous, curvy and wrinkled upon drying, up to 2 cm long and 7 mm diam. Hyphal system dimitic; generative hyphae bearing both clamp connections and simple septa; skeleto-binding hyphae IKI–, CB–; tissues unchanged in KOH. Contextual generative hyphae frequent, colourless, thin-walled, bearing clamp connections, frequently branched, 2.5–8 lm diam., usually inflating at the branching areas or clamping areas, up to 14 lm in diam.; contextual skeleto-binding hyphae dominant, colourless, thick-walled with a narrow to wide lumen, frequently branched, flexuous, interwoven, 2.5–6.5 lm diam., occasionally inflated up to 11 lm diam.; contextual cuticle hyphae simple septate, thin-walled, frequently branched, interwoven, 1.5–4.5 lm diam. Tramal generative hyphae frequent, colourless, thin-walled, bearing clamp connections and simple septa, infrequently branched, 2.5–4 lm diam.; tramal skeleto-binding hyphae dominant, colourless, thick-walled with a narrow to wide lumen, frequently branched, flexuous, interwoven, 1.3–4 lm diam. Stipe generative hyphae frequent, colourless, thin-walled, bearing clamp connections, occasionally branched, 1.5–6 lm diam.; stipe skeleto-binding hyphae dominant, colourless, thick-walled, subsolid or with a narrow to wide lumen, moderately branched, flexuous, interwoven, 2.5–6 lm diam.; stipe cuticle generative hyphae dominant, with buff inclusion inside, thick-walled with a wide lumen, bearing clamp connections; stipe cuticle skeleto-binding hyphae frequent, with light brown to orange brown inclusion inside, thick-walled with a narrow lumen, occasionally branched, 2.4–4.5 lm diam. Cystidia and cystidioles absent; basidia clavate, with a basal clamp and four sterigmata, 16.5–24 9 7–10 lm; basidioles in shape similar to basidia, but smaller and without sterigmata. Basidiospores cylindrical, few, oblong, colourless, thin-walled, smooth, with guttules, IKI–, CB–, (6.5–)7.5–10.5(–11) 9 3.5–4.5 (–5) lm, L = 8.7 lm, W = 4.07 lm, Q = 1.84–2.53, Qm = 2.14 (n = 60/1). Type of rot: White rot. Specimens examined: CHINA, Hunan Province, Yizhang County, Mangshan Nature Reserve, on fallen angiosperm branch, 17 August 2014, Dai 15151 (BJFC 018267, holotype). Notes: Recently, taxonomic and phylogenetic studies of wood-rotting fungi in subtropical China have been carried out, and many new species have been described based on both morphological characters and molecular data (Chen and Cui 2014, 2016; Chen et al. 2015c, 2016; Han et al. 2016; Li and Cui 2013; Li et al. 2014; Song et al. 2014, 2016; Zhao et al. 2013, 2015a). In the current study, Polyporus mangshanensis is an additional new species described from subtropical China on the basis of morphological characters and phylogenetic analysis. Morphologically, P. mangshanensis is characterized by its beige to yellowish-orange pileal surface with slightly radial stripes, 123 Fungal Diversity (2016) 80:1–270 brown beige to olive brown pore surface, dark brown stipe, generative hyphae bearing both clamp connections and simple septa, and oblong to cylindrical basidiospores (7.5–10.5 9 3.5–4.5 lm). Phylogenetically, P. mangshanensis clustered with P. leprieurii Mont. and P. guianensis Mont.; morphologically, they produce similar pileal surface, pore surface and dark stipe, however, P. mangshanensis differs from the latter two species by producing both clamped and simple-septate generative hyphae, contextual generative hyphae and skeleto-binding hyphae usually inflated over 10 lm in diam. Moreover, pores and basidiospores of P. mangshanensis are smaller than P. guianensis (pores 1–2 per mm, basidiospores 8–12 9 2.5–4 lm), while larger than P. leprieurii (pores 5–8 per mm, basidiospores 4.5–7 9 2–2.5 lm; Núñez and Ryvarden 1995). Polyporus subvarius C.J. Yu & Y.C. Dai has a similar pileal and pore surface to P. mangshanensis, but the former has larger pores (1–2 per mm) and basidiospores (9.2–12.6 9 3.9–4.9 lm, L = 10.7 lm, W = 4.48 lm), only clamped generative hyphae, and grows on living trees of Salix (Dai et al. 2007). Polyporus admirabilis Peck resembles P. mangshanensis in having a laterally dark stipe, a tan pileal surface and similar pore size, but it differs in its subulate cystidioles, only clamped generative hyphae, and slender basidiospores (7.8–9 9 3–3.5 lm, L = 8.29 lm, W = 3.12 lm, Qm = 2.66; Dai 1999). Both Polyporus badius (Pers.) A.B. De and P. submelanopus H.J. Xue & L.W. Zhou have simple-septate generative hyphae, dark stipe and cylindrical basidiospores, which are similar to P. mangshanensis. Polyporus badius has darker pileal surface and smaller pores (5–6 per mm), only simple-septate generative hyphae (Dai 1999); while P. submelanopus has larger pores (2–3 per mm) and basidia (24–33 9 5–8 lm), and a terrestrial habit (Xue and Zhou 2012). Russulales Kreisel ex P.M. Kirk et al. Russulaceae Lotsy The family Russulaceae is one of the dominant and morphologically diverse (Miller et al. 2006) ectomycorrhizal mushroom families in the Himalayas. Apart from its three corticoid genera, i.e. Boidinia, Gloeopeniophorella and Pseudoxenasma (Larsson and Larsson 2003; Miller et al. 2006), this family has four predominantly agaricoid genera: Lactifluus, Lactarius, Multifurca and Russula (Buyck et al. 2008, 2010), some of which may also contain secotioidhypogeous or pleurotoid species. Russula Pers. The monophyletic agaricoid genus Russula (Buyck et al. 2008, 2010) has an enormous diversity in Indian Himalaya Fungal Diversity (2016) 80:1–270 (Rawla 2001; Das and Sharma 2005; Das et al. 2006, 2010, 2013, 2014) showing its wide range of distribution from tropical to subalpine areas and associations with broadleaf to coniferous trees. Apart from morphology-based six subgenera (Sarnari 1998; R. subg. Compactae, R. subg. Heterophyllidia, R. subg. Ingratula, R. subg. Amoenula, R. subg. Incrustatula and R. subg. Russula), three additional subgenera have been recently described (Hongsanan et al. 2015a; R. subg. Archaea Buyck & V. Hofstetter, R. subg. Brevipes Buyck & V. Hofstetter and R. subg. Malodora Buyck & V. Hofstetter). Here, two novel species (belonging to R. subg. Heterophyllidia and R. subg. Amoenula respectively), collected from the northwestern part of Indian Himalaya are introduced together with their morphology and phylogenetic placement (Figs. 170, 173). Russula indoalba A. Ghosh, Buyck, A. Baghela, K. Das & R.P. Bhatt, sp. nov. Index Fungorum number: IF552159; Facesoffungi number: FoF02471, Figs. 171 and 172 Etymology: referring to white basidiomata growing in India Holotype: CAL 1328. Basidiomata 70–100 mm. in height. Pileus 30–95 mm. in diam., broadly convex when young, gradually planoconvex to applanate with depressed center, finally uplifted at maturity; margin decurved to plane, entire, tuberculately striate; surface dry, viscid when moist, cracked-areolate when mature, white (1A1–2A1) with greyish-yellow (4B4–4B6) to pale yellow (3A3) spots at the center; lemon-yellow or pale yellow to pastel yellow (1A3–1A4) with KOH; cap context white (1A1–2A1), lemon-yellow or pale yellow to pastel yellow (1A3–1A4) with KOH. Lamellae adnexed to almost free with age, equal, subdistant to close (7–10/cm), forked near the stipe apex, white (1A1–1A2), with entire, concolourous gill edges. Stipe 20–65 9 10–23 mm., subclavate, central, dry, smooth, white (1A1–2A1), lemonyellow or pale yellow to pastel yellow (1A3–1A4) with KOH; context stuffed, becoming hollow with age, white (1A1–2A1), greyish-orange (5B4–5B5) with FeSO4, unchanging with guaiacol and NH4OH. Taste mild. Odour indistinctive. Spore print yellowish-cream. Basidiospores 5.5–7.42–9.5 9 5.5–6.4–8 lm (n = 50, Q = 1–1.16– 1.36), subglobose to broadly ellipsoid, rarely ellipsoid; ornamentation amyloid, composed of short (0.3–0.5 lm) and long (0.7–1 lm) conical to cylindric warts, mostly connected to form incomplete reticulum, few isolated, apiculi up to 2 lm high; suprahilar spot not amyloid. Basidia 45–60 9 9–13 lm, 4-spored, cylindrical to subclavate, sterigmata up to 5 lm long. Pleurocystidia 47–90 9 8–16 lm, subclavate to clavate with capitate, moniliform, appendiculate and rounded apex, emergent up to 38 lm beyond the basidiole tips. Cheilocystidia 251 55–68 9 6–8 lm, cylindrical to subclavate with appendiculate apex. Subhymenium layer up to 26 lm thick, pseudoparenchymatous. Lamellar trama consists predominantly of sphaerocytes. Gill edges fertile. Pileipellis up to 120 lm thick, distinctly divided into suprapellis and supellis; subprapellis composed of erect to suberect chains of 3–6 rows of cells; subterminal cells 8–20 9 6–18 lm, mostly rounded (inflated), ellipsoid; terminal cells mostly, cylindrical to subulate; subpellis composed of. horizontal, interwoven hyphae. Pileocystidia rare, one-celled, slender, thin-walled, up to 7 lm wide, dispersed as terminal cells in the suprapellis. Habitat and distribution: Under Quercus sp. in mixed forests dominated by Quercus, Rhododendron, Abies and Cupressus. Material examined: INDIA, Uttarakhand, Rudraprayag district, Baniyakund, alt. 2630 m, N30°28.9140 0 E79°10.854 , 1 September 2014, A. Ghosh, AG 15-541 (GUH); ibid., 14 July 2015, A. Ghosh, AG 15-628 (CAL 1328, holotype); ibid., A. Ghosh, 16 July 2015, AG 15-661 (GUH); ibid., 1 August 2015, A. Ghosh, AG 15-797 (GUH). Notes: Russula indoalba which is characterized by white pileus with greyish-yellow to pale yellow spots at center, tuberculately striate and wavy to interrupted pilear margin, equal gills, yellowish-cream spore print and mild taste is a typical member of Russula subg. Heterophyllidia sect. Virescentinae. In the field Russula kanadii A.K. Dutta & K. Acharya (also reported from India) appears to be quite similar to the present taxon. However, R kanadii has a white spore print (1A1), smaller basidiospores [(4.5)–5.5–5.7–6.5 (–7) 9 (4.5)–5.3–5.5(–6) lm] and grows at very low altitudes (56 m) being ectomycorrhizal with dipterocarps, viz. Shorea robusta (Dutta et al. 2015). Russula alboareolata Hongo, described from Japan in association with Castanopsis (Fagaceae), but also reported from dipterocarps in Taiwan (Watling and Lee 1998) and Thailand is also very similar to R. indoalba but has smaller basidiospores 6.5–8.5 9 5.5–7 lm (Hongo 1979). Our phylogeny (Fig. 170) shows that R. alboareolata is genetically closer to R. kanadii than to our new species or to the other Virescentinae (Fig. 170). Furthermore, the phylogeny also suggests, although without support, that R. indoalba is basal to the core group of Virescentinae (R. virescens and allies) suggesting a migration from India via Asia (Fig. 170, sequence UDB014226) to both Europe and North America. All species in this core-group differ from other Virescentinae such as those belonging to the R. crustosa-R. mustelina lineage and also R. parvovirescens Buyck et al. (Buyck et al. 2006) in the absence of dermatocystidia in the lower subpellis (Buyck, http:// www2.muse.it/russulales-news/id_virescentinae.asp). 123 252 Russula pseudoamoenicolor A. Ghosh, Buyck, K. Das, A. Baghela & R.P. Bhatt, sp. nov. MycoBank No.: MB 817101; Facesoffungi number: FoF02522, Figs. 174 and 175 Etymology: referring to the lookalike of Russula amoenicolor, an European species. Holotype: CAL 1330. Basidiomata up to 100 mm in height. Pileus 50–100 mm in diam., globose, plano-convex to applanate with broadly depressed center, becoming uplifted when mature; margin decurved, entire, tuberculately striate, torn when mature, surface dry, viscid when moist, subvelvety, purplish-red-violet-red (14B5–14B8) or light lilac, light violet to pastel violet (16A4–16A5) with purplish-white (14A2) to violet-white (16A2) towards margin, rarely dark violet (15A6–15A8) towards the depression; cuticle peeling 3/4th of the radius; cap context white (1A1–2A1), unchanging when bruised. Lamellae adnexed to subdecurrent, close to rather crowded, white (1A1–2A1), forked near the stipe; edges marginate near the cap margin, lamellulae absent. Stipe 45–70 9 10–14 mm, equal, slightly tapered towards the base, dry, smooth, brittle, central, reddish-white to pink-rose or pink-red (12A3–12A5), context stuffed, white (1A1–2A1), pinkrose (12A3–12A5) with guaiacol. Taste mild. Spore print not obtained. Basidiospores 6–7.30–9.5 9 5–6.33–8 lm (n = 25, Q = 1.03–1.16–1.33), subglobose to broadly ellipsoid, rarely ellipsoid, ornamentation amyloid, composed mostly of ridges and warts (up to 1 lm high) aligned or connected to form an incomplete reticulum, with few isolated warts, apiculi up to 2 lm high. Basidia 35–55 9 9–13 lm, cylindrical, subclavate to clavate, 4-spored, sterigmata up to 6 lm high. Subhymenium layer up to 30 lm thick, pseudoparenchymatous. Hymenophoral trama mainly consisting of sphaerocytes measuring 17–40 9 16–34 lm. Pleurocystidia 90–117 9 10–21 lm, ventricose, subfusiform to fusiform with blunt apex, thick walled (1 lm thick), emergent up to 60 lm; content blank or insignificant. Gill edges fertile, with basidia and cystidia. Cheilocystidia 30–85 9 7–10 lm, same as pleurocystidia. Pileipellis up to 90 lm thick, composed of clustered erect to suberect elements composed of chains of 4–7 cells; terminal cells ellipsoid, conical, subfusoid to occasionally subulate (11–65 9 4–10 lm wide); subterminal cells mostly cylindrical to rectangular, few ellipsoid or rounded (inflated), measuring up to 14 lm wide. Habitat and distribution: grows in close association with Quercus sp. with undergrowth of Rhododendron sp. in moist deciduous and mixed (broadleaf and coniferous) forest. Material examined: INDIA, Uttarakhand, Pauri Garhwal, along the road side of khirsu, alt. 1835 m., 123 Fungal Diversity (2016) 80:1–270 N30°10.1550 E78°52.1340 , 24 July 2015, A. Ghosh, AG 15-739 (CAL 1330, holotype) Notes: The combination of characters in Russula pseudoamoenicolor comprising a purplish-red to violet-red or lilac subvelvety pileus with darker center, reddish-violet to pink-rose stipe, occasional occurrence of typically subulate terminal cells of pileipellis, absence of dermatocystidia and inamyloid suprahilar spot place it in R. subg. Amoenula Sarnari. In the field, the European species, R. amoenicolor Romagn. appears to be quite similar to the present taxon but the former (Sarnari 1998) has purple to green or variegated pileus, smaller basidiospores (6.7–8.4 9 5.6–7.4 lm) and mostly subulate terminal cells in pileipellis (terminal cells mainly ellipsoid, conical, subfusoid or occasionally subulate in R. pseudoamoenicolor) and its ITS nucleotide sequence are dissimilar (93 % identity with R. pseudoamoenicolor for 100 % query coverage using BLAST) from the present species. From a phylogenetic standpoint, R. pseudoamoenicolor is closely related to R. violeipes and cluster together in a subclade with high bootstrap support (Fig. 173). The European species, R. violeipes Quél. was recently reported from South Korea (Park et al. 2013), yet these collections differs significantly from European material (GenBank acc. no. AY061726). These Korean collections (GenBank accession nos. KF361797 and KF361783) are here, however, recovered as identical to our material from the Indian Himalaya. Interestingly, two Australian species, R. variispora T. Lebel and R. rostraticystidia T. Lebel, previously of undetermined position within R. subg. Heterophyllidia (Lebel and Tonkin 2007) are here for the first time recovered with significant support (93 % BS) as very closely related to or likely members of R. subsect. Amoeninae. Russula variispora and R. rostraticystidia both are sequestrate species and hence, have an altogether different morphology from other known Amoeninae, or even from all other known species in R. subg. Heterophyllidia as secotioid-gasteroid taxa have never been reported from other subsections in this subgenus (Kong et al. 2015). The rostrate pleurocystidia of R. rostraticystidia and the similarly shaped terminal cells in the less developed pileipellis of these Australian secotioid species (Lebel and Tonkin 2007) support their placement in Amoeninae. Lactarius Pers. The well-known milkcaps are split into two genera: Lactarius and Lactifluus (Buyck et al. 2008, 2010). The genus Lactarius comprises three subgenera, L. subg. Piperites (Fr. ex J. Kickx f.) Kauffman, L. subg. Russularia (Fr. ex Burl.) Kauffman and L. subg. Plinthogali (Burl.) Hesler & A.H. Sm. and is one of the common ectomycorrhizal associates in Indian Himalaya (Rawla 2002; Das Fungal Diversity (2016) 80:1–270 and Sharma 2005; Das and Verbeken 2011, 2012; Das and Chakraborty 2014; Das et al. 2015). One undescribed species of L. subg. Plinthogali collected from northwest part of Indian Himalaya is introduced here with morphological details and phylogenetic evidence. A phylogenetic tree is presented in Fig. 176. Lactarius dirkii Uniyal, K. Das, A. Baghela & R.P. Bhatt, sp. nov. MycoBank: MB 817126; Facesoffungi number FoF02521, Figs. 177 and 178 Etymology: commemorating Dr. Dirk Stubbe for his contribution to Lactarius subg. Plinthogali. Holotype: CAL 1332. Pileus 20–86 mm. in diam.; convex when young, becoming planoconvex with shallowly depressed center with maturity; margin decurved, entire to irregularly undulating, often interrupted; surface dry, slightly wrinkled towards margin when mature; white to orange-white (6A2) with a pinkish-white (7A2) or paler tinge, spotted with reddish-grey (7B2) scrobicules, changing to light yellow (2A5) with 10 % KOH. Lamellae subdecurrent, close to rather crowded (13–14/10 mm), some forked near the stipe apex, white to orange-white (5A2) to pale orange (5A3), becoming pinkish to pale red (7A2) to pastel red (7A3/4) when bruised or cut, lamellulae numerous. Stipe 30–58 9 5–8 mm., cylindrical, tapering toward base, central, dry, concolourous with pileus, white at base with minute hairs; Context whitish to yellowish-white (4A2), turning light orange on cutting, hollow in stipe; Latex abundant, white, unchanging when isolated, but drying pinkish on cut lamellae. Taste bitter to acrid. Odour insignificant. Spore print light yellow (4A5). Basidiospores 6.5–7.7–8.5 9 6–6.9–7.5 lm, (n = 70, Q = 1.06–1.11– 1.21), subglobose to broadly ellipsoid, rarely broadly ellipsoid, winged; ornamentations amyloid, composed mostly of broad wings and few small ridges but never forming a reticulum, up to 2 lm high, edges smooth, isolated warts also present between ridges, plage amyloid. Basidia 44–62 9 11–14 lm, subclavate, 4-spored, sterigmata 4–7 lm long. Hymenophoral trama composed of lactifers and rosettes of sphaerocytes. Pleurocystidia absent. Pseudocystidia abundant, emergent, cylindrical, sometimes tortuous, branched, up to 5.5 lm wide. Lamellar edge sterile. Cheiloleptocystidia 24–35 9 4–5.5 lm, mostly cylindrical, rarely subfusiform, thin walled. Subhymenium up to 20 lm thick, pseudoparenchymatous. Lactifers in hymenophoral trama up to 7 lm wide. Pileipellis trichopalisade, 55–121 lm thick, suprapellis formed of cylindrical to subfusiform cells and septate hyphal elements 10–57 9 3.5–6.5 lm; subpellis composed of cells with irregular shapes, cells up to 11 lm wide; Lactifers in pilear trama up to 8 lm wide, sometimes 253 becoming gradually thin toward suprapellis and projecting in form of pileopseudocystidia, wide up to 4.5 lm. Stipitipellis trichoderm, up to 100 lm thick, hyphae up to 5 lm wide. Habitat & distribution: Under Quercus sp., Rhododendron arboreum in temperate mixed forest dominated by Abies, Cupressus, Quercus and Rhododendron. Material examined: INDIA, Uttarakhand, Rudraprayag district, Baniyakund, 2630 m, N30°28.9140 E79°10.8540 , 29 August 2015, P. Uniyal, PU 15-1004 (CAL 1332, holotype); ibid., Baniyakund, 2630 m, N30°28.9140 E79°10.8540 , 9th Aug 2014, P. Uniyal, PU 15-360 (GUH); ibid., 25 August 2014, P. Uniyal, PU 15-451 (GUH); ibid., 1 August 2015, P. Uniyal, PU 15-803 (GUH). Notes: The combination of morphological features such as whitish dry pileus, concolourous (to pileus) stipe, light yellow spore print, winged basidiospores, trichopalisade pattern of pileipellis and absence of hymenial macrocystidia undoubtedly place Lactarius dirkii under L. subg. Plinthogali (Burl.) Hesler & A.H. Sm. (Basso 1999; Das and Sharma 2004; Stubbe et al. 2008). Among this subgenus, the new species is fairly easy to recognize in the field by its white to orange-white basidiomata with pinkish tinge and darker scrobicules on cap surface, yellow reaction with KOH, close to rather crowded lamellae turning pinkish to orange on bruising, hollow stipe and abundant white latex that remains unchanged when isolated but becoming pinkish on cut lamellae. Micromorphologically, occurrence of subglobose to broadly ellipsoid basidiospores with up to 2 lm high wing like ornamentations and warts that never form a reticulum, abundant emergent pseudocystidia and sterile lamellar edges containing cylindrical cheiloleptocystidia are also worth mentioning. In the field, L. dirkii can be mistaken for L. oomsisiensis Verbeken & Halling (probably morphologically closest species, which was reported from Papua New Guinea and Thailand and labeled with GenBank accession numbers EF560680 and EF560679, respectively in Fig. 176). Both have whitish basidiomata but the latter can be distinguished macromorphologically by distant orange-brown lamellae, white latex drying cream on gills and mild taste (Le et al. 2007) and micromorphologically, by comparatively narrow pileipellis (40–80 lm thick) with brown intracellular pigmentation in upper layer and absence of pileopseudocystidia in suprapellis. Lactarius dirkii is also closely related to another Asian taxon, L. friabilis H.T. Le & Stubbe (reported from Thailand as well and labelled with GenBank accession numbers EF560663 and EF560664 but, the latter differs from L. dirkii in having distant (to subdistant) lamellae, larger spores (7.8–7.9–8.4–9.1 9 7.1– 7.5–7.8–8.7 lm) with incompletely reticulated ornamentation of warts and ridges with mostly crenulate edges (Le et al. 2007). Phylogenetically, both L. oomsisiensis (95 % 123 254 identity with L. dirkii for 95 % query coverage using BLAST) and L. friabilis (94 % identity with L. dirkii for 98 % query coverage using BLAST) are also distinct. In L. subg. Plinthogali, molecular phylogeny already confirmed the existence of eight European species (Stubbe and Verbeken 2012) of which, seven species: L. acris (Bolton: Fr.) Gray (GenBank acc. nos. JQ446084 and JQ446083), L. azonites (Bull.) Fr. (GenBank acc. nos. JQ446094 and JQ446095), L. fuliginosus (Fr.: Fr.) Fr. (GenBank acc. nos. JQ446111 and JQ446110), L. picinus Fr. (GenBank acc. nos. JQ446129 and JQ446130), L. pterosporous Romagn. (GenBank acc. nos. JQ446138 and JQ446136), L. romagnesii Bon (GenBank acc. nos. JQ446143 and EF560662) and L. ruginosus Romagn. (GenBank acc. nos. JQ446106 and EF560660) are found to be closely related (93–95 % identity with L. dirkii for 95–98 % query coverage using BLAST) to L. dirkii (showing significant support with the Asian taxa of L. subg. Plinthogali in Fig. 176). However, mostly these species are dark coloured in contrast to the white basidiome of L. dirkii, making it easily distinguishable from them in the field. Moreover, L. acris shows a completely different pattern of pileipellis i.e., ixooedotrichoderm to ixotrichopalisade with capitate terminal elements (HeilmannClausen et al. 1998); L. fuliginosus and L. picinus (also reported from India) have dark coloured cap, scarce latex, trichoepithelium pattern of pileipellis and lower (1 lm) spore ornamentations (Heilmann-Clausen et al. 1998) and strictly conifer association in later species (Stubbe and Verbeken 2012). Among other close European taxa, L. romagnesii and L. ruginosus have fuscous colour of pileus, irregularly crenate to grooved margin, distant gills and rather sparse latex and spores with high ornamentations that are reticulate in L. romagnesii but zebroid in L. ruginosus (Stubbe and Verbeken 2012), while L. azonites can be separated from the present taxon by distant gills, larger spores (7.3–9.3 9 6.8–8.3 lm), lower ornamentations (up to 1 lm) and hyphoepithelium to trichoepithelium type of pileipellis (Heilmann-Clausen et al. 1998). Lactarius pterosporus shares similar pileipellis structure (a trichopalisade of 80–120 lm thick), but differs in having dark greyish-buff coloured cap, sparse latex and higher (up to 2.5 lm) spore ornamentations (Heilmann-Clausen et al. 1998). Molecular data also indicates genetic closeness (95 % identity with L. dirkii for 95 % query coverage using BLAST) of L. dirkii with L. fumosibrunneus A.H. Sm. & Hesler (reported from North American continent and labelled with GenBank acc. nos. JQ797634 and JQ797633 in Fig. 176), which is different from the former in brownish, rugose cap, negative reaction of KOH on surface and reddening of tissue by latex. Micromorphologically, hymenoepithelium nature of pileipellis and presence of 123 Fungal Diversity (2016) 80:1–270 abundant cheilocystidia in L. fumosibrunneus are also quite distinct (Bandala and Montoya 2010). Few Indian species of L. subg. Plinthogali namely, L. crenulatus K. Das & Verbeken, L. montoyae K. Das & J.R. Sharma, L. croceigalus K. Das & Verbeken and L. vesterholtii K. Das & D. Chakr. partly resemble micromorphologically (nature of pileipellis, absence of macrocystidia, pinkish discoloration of flesh and high spore ornamentations). But, L. crenulatus has distinctly smaller papillate dark-coloured pileus and slender stipe (Das and Verbeken 2012). Lactarius montoyae (also reported from Thailand) shows subdistant to distant lamellae, brown coloured basidiomata, white unchanging latex which does not stain tissues (Le et al. 2007; Das and Sharma 2005). Lactarius croceigalus differs from L. dirkii on the basis of larger, papillate and dark colored pileus, distant lamellae and larger spores (9–9.2–10.1 9 7.8–8.7–9.5 lm) as mentioned by Das and Verbeken (2012). Finally, Lactarius vesterholtii, which was recently discovered from Himalayan India under L. subg. Plinthogali, can be separated morphologically from the present species by crowded lamellae, typically orange discolouration of latex on exposed gills, brown to greyish-brown pileus, lack of pileopseudocystidia and showing palisade to lampropalisade nature of stipitipellis (Das and Chakraborty 2014). Mortierellomycotina Kerst. Hoffm. et al. Mortierellales Caval.-Sm. Mortierellaceae A. Fisch. We follow Li et al. (2016). Mortierella Coem. The genus Mortierella (Mortierellaceae, Mortierellales) was described by Coemans (1863) with type species M. polycephala Coem. To date, nearly 100 species of Mortierella have been described (Wagner et al. 2013). The species belonging to this genus are characterized by the production of a mainly coenocytic but becoming irregularly septate mycelium. Sporangiophores are simple or variously branched terminating with sporangia and occasionally with a swelling at the base. Sporangia are globose, multi-, few- or uni-spored. Species of Mortierella typically exhibit rapid growth at temperatures ranging from 15 °C to 25 °C. They are frequently isolated from soil and dead or dying plant tissue or from animal dung (Gams 1977; Benny 2006, 2008). Many of them show potential as producers of polyunsaturated fatty acids (Shinmen et al. 1989; Ogawa et al. 2012). In addition, several species of Mortierella have been used as pesticide degrading agent, suggesting that they might have potential for the bioremediation of sites contaminated with organochlorine pesticides (Kataoka et al. 2010). Fungal Diversity (2016) 80:1–270 Based on morphological characters, Gams (1977) divided Mortierella into nine sections: Actinomortierella, Alpina, Haplosporangium, Hygrophila, Mortierella, Schmuckeri, Simplex, Spinosa and Stylospora. Recently, molecular data have been used to evaluate the genus Mortierella (Petkovits et al. 2011; Wagner et al. 2013; Ariyawansa et al. 2015a, b, c). While examining the diversity of fungi of the order Mortierellales isolated from freshwater sample of Yeongsan River in Gwangju, Korea, a new species was isolated and is described here based on morphological characteristics and phylogenetic analyses. Mortierella fluviae Hyang B. Lee, K. Voigt & T.T.T. Nguyen, sp. nov. MycoBank number: MB 817071; Facesoffungi number: FoF02474, Fig. 181 Etymology: referring to the freshwater which from the species was first isolated Holotype: EML-YR25716-1. Colonies reaching 45–48 mm diam. at 20 °C after 7 days incubation on PDA, cotton in the center with a white margin, the reverse white and regularly zonate: on OA, aerial hyphae dispersed on agar surface. Sporangiophores 115–350 lm long, 3.5–7.5 lm wide at the tip, 8.5–17 lm wide at the base, arising from the aerial stolons with 1–4 branches (av. 2–3). Sporangia 19–51 9 23–52 lm, globose, multi-spored, with deliquescent wall, sometimes with a bell-shaped apophysis. Columellae 3.5–8 9 5–10 lm, hemisphaerical or subglobose, with small projection on the apex. Collarette appearing after sporangium maturation. Spores globose to ellipsoidal or pyriform, 6.5–11.5 9 5.5–8.5 lm. Chlamydospores absent in aerial mycelia. Zygospores not observed. The isolate was observed to grow over a wide range of temperatures with varying growth rates on PDA, OA (oatmeal agar), and WA (water agar). The average growth rates on PDA, OA, and WA were 6.5 mm, 6 mm, and 5 mm per 24 h, respectively. Optimal growth was observed around 15–25 °C, maximum around 30 °C, and no growth at 35 °C (Fig. 181). Notes: Mortierella fluviae is similar to M. gamsii but differs by shorter sporangiospores and having a bell-shaped apophysis. Spores are variable in shape. In the phylogenetic trees the ITS and 28S rDNA sequences of the strain formed a branch separate from other species of Mortierella, showing it represents a new species (Figs. 179, 180, respectively). Material examined: REPUBLIC OF KOREA, Jeonnam Province, Yeongsan River located in Gwangju (35°100 N 126°550 E), from a freshwater sample, 15 February 2016; holotype, ex-type living culture EML- 255 YR25716-1 at Culture Collection of Nakdonggang National Institute of Biological Resources (NNIBR), Sangju, Gyeongbuk Province, and preserved as glycerol stock at -80 °C in the Chonnam National University Fungal Collection (CNUFC) under deposition number (CNUFC-EML-YR25716-1) and living culture (ex-type) deposited at Jena Microbial Resource Collection (University of Jena and Leibniz Institute for Natural Product Research and Infection Biology, Jena, Germany) (JMRC:SF:012332). Mucoromycotina Benny Mucorales Fr. Cunninghamellaceae Naumov ex R.K. Benj. Cunninghamella Matr. Cunninghamella includes saprobes species commonly isolated from soil, decaying fruit or rotten wood, and some species have been responsible for disseminating infections in humans, especially immunocompromised patients (Yu et al. 2015). Species of Cunninghamella have traditionally been distinguished based on their morphology, especially in their sporangial stages (Baijal and Mehrotra 1980; Zheng and Chen 2001; Yu et al. 2015). Since 1991, other characteristics, such as maximum growth temperature, the color and texture of colonies, mating compatibility, zygospore formation as well as molecular tools have been used to delimitate the species (Zhou and Huang 1991; Zheng and Chen 1994, 1998; Liu et al. 2001; Gherbawy and Voigt 2010). Zheng and Chen (2001) monographed the genus based on morphological characteristics, maximum growth temperature and mating experiments, as well as the entire length of the ITS region, describing twelve species and three varieties: C. bertholletiae Stadel, C. blakesleeana Lendn., C. binarie R.Y. Zheng, C. clavata R.Y. Zheng & G.Q. Chen, C. echinulata var. antarctica (Caretta & Piont.) R.Y. Zheng & G.Q. Chen, C. echinulata var. echinulata (Thaxt.) Thaxt. ex Blakeslee, C. echinulata var. nodosa R.Y. Zheng, C. echinulata var. verticillata (F.S. Paine) R.Y. Zheng & G.Q. Chen, C. elegans Lendn., C. homothallica Komin. & Tubaki, C. intermedia K.B. Deshp. & Mantri, C. multiverticillata R.Y. Zheng & G.Q. Chen, C. phaeospora Boedijn and C. septata R.Y. Zheng. Liu et al. (2011) and Yu et al. (2015) amplified the ITS rDNA region and TEF-1a of all the species described by Zheng and Chen (2001) and found their results to be consistent with morphological studies performed by previous authors. Cunninghamella gigacellularis A.L. Santiago, C.L. Lima & C.A. de Souza, sp. nov. Index Fungorum number: IF552131; Facesoffungi number: FoF02204, Fig. 184 123 256 Etymology: gigacellularis. A reference to the giant cells produced in the mycelium Holotype: URM 7400. Colonies white, floccose, reaching 9 cm diam. and touching the plate lid after 4 days on BDA at 25 °C. Reverse light yellow. Rhizoids frequent, long or short, simple or weakly branched. Stolons present, coenocytic, some with septa near the site of origin of the sporophore. Sporophores erect, straight or recumbent, smooth-walled, arising from stolons or from aerial hyphae; main axes of the sporophores usually equal in width throughout, (5–) 6.5–10(–12.5) lm diam., predominantly terminated with a vesicle, but with some dividing in branches at the apices; branches monopodial, verticillate and pseudoverticillate; short and long branches (up to 500 9 7.5 lm) in the same sporophore are common, repeatedly branching 1–6 times, terminating with a vesicle. Some branches arise from a bulbous base. Septa in sporophores may be present at its base, near to the branches of the sporophore or below the vesicles. Vesicles of the main sporophores light grey, smooth-walled, globose and subglobose, some lightly depressed, but rarely obovoid or piriform, (14–)20–35 lm diam. Lateral vesicles light grey, smooth-walled, globose and subglobose (10–)12.5–20(–25) lm diam. Sporangioles hyaline, subsmooth to very shortly echinulate, mostly globose 5–7.5 lm diam., some subglobose and ellipsoid (12.5–)7.5–9 9 6–8(–10) lm, rarely with a pointed end. The larger sporangioles are light grey to pale brown and more echinulate than the smaller ones. Giant cells subglobose, some hypha-like and irregularly swollen, simple or branched. Zygosporangia not observed. Material examined: BRAZIL, Itambé: Instituto Agronômico de Pernambuco (7°970 6000 S, 35°470 8800 W), in soil samples. Soil, 11.X.2013, leg. C. Lima (URM 7400, holotype) and deposited in the Jena Microbial Resource Collection (University of Jena and Leibniz Institute for Natural Product Research and Infection Biology, Jena, Germany) (JMRC:SF:012269). Media and temperature tests: On BDA. At 15 °C—very limited growth (2.6 cm in diam. in 120 h); total lack of reproductive structures. At 20 °C—slow growth (5 cm in 120 h); good sporulation. At 25 °C—good growth (8 cm in 120 h); good sporulation. At 30 and 35 °C—excellent growth (9 cm in 96 h); excellent sporulation. At 40 °C— 6.8 cm in 96 h; rare sporophores formed, very poor sporulation. At 42 °C—total lack of growth and sporulation. The growth of C. gigacellularis on MEA was slightly slower than on PDA at all tested temperatures, except at 25 °C. Abundant sterile mycelium was observed on PDA and MEA cultures at 30, 35 and 40 °C. Notes: Cunninghamella gigacellularis is distinguished from other species of the genus as it produces giant cells that have not so far been reported for this genus. The new 123 Fungal Diversity (2016) 80:1–270 species also simultaneously presents a white colony and produces monopodially, verticillately and pseudoverticillately branched sporophores, as well as producing long and short branches in the same repeatedly branched sporophore. At first, C. gigacellularis could be confused with C. bertholletiae Stadel because of the branching pattern of the sporophores. However, only the former produces giant cells. Colonies of C. gigacellularis are persistently white, in contrast to the drab to mouse grey colonies of C. bertholletiae (Zheng and Chen 2001), and sporophores of C. gigacellularis seem to be more repeatedly branched than those of C. bertholletiae. Additionally, the sporangiospores of C. bertholletiae are 4.5–9(–11) or 5–14.5 (–16) 9 3.5–9(–11) lm (Zheng and Chen 2001), larger than those of C. gigacellularis. Our molecular analysis (ITS and LSU rDNA regions, Figs. 182, 183, respectively) placed C. gigacellularis in a separate clade in ITS and LSU trees, confirming that it is genetically different from other species of the genus. Acknowledgements K.D. Hyde would like to thank the Thailand Research Fund grant no RSA5980068 entitled Biodiversity, phylogeny and role of fungal endophytes on above parts of Rhizophora apiculata and Nypa fruticans and the Chinese Academy of Sciences, Project Number 2013T2S0030, for the award of Visiting Professorship for Senior International Scientists at Kunming Institute of Botany. Financial support by the German Academic Exchange Service (DAAD) and the Thai Royal Golden Ph.D. Jubilee-Industry program (RGJ) for a joint TRF-DAAD PPP (2012–2014) academic exchange grant to K.D. Hyde and M. Stadler, and the RGJ for a personal grant to B. Thongbai (No. Ph.D/0138/2553 in 4.S.MF/53/A.3) is gratefully acknowledged. Chayanard Phukhamsakda (PHD/0020/2557) acknowledges the The Royal Golden Jubilee Ph.D. Program under the Thailand Research Fund. Mingkwan Doilom acknowledges the Royal Golden Jubilee Ph.D. Program (PHD./0072/2553 in 4.S.M.F./53/A.2) under the Thailand Research Fund. Ausana Mapook is grateful to Research and Researchers for Industries (RRI) PHD57I0012. Rungtiwa Phookamsak sincerely appreciates The Royal Golden Jubilee Ph. D. Program (PHD/0090/2551 in 4. S. MF/51/A.1) under the Thailand Research Fund for financial support. Qi Zhao thanks the National Natural Science Foundation of China (No. 31360015) and the CAS/SAFEA International Partnership Program for Creative Research Teams, and the Knowledge Innovation Program of the Chinese Academy of Sciences (No. KSCX2-EW-Z-9 and KIB2016002). KNAR acknowledges support from the University Grants Commission (UGC), India, in the form of a Rajiv Gandhi National Fellowship (Grant No. F. 14-2(SC)/2009 (SA-III)) and the permissions given to him for collecting agaric specimens from the forests of Kerala by the Principal Chief Conservator of Forests, Government of Kerala (WL12-4042/2009 dated 05-08-2009). This Project was funded by the National Plan for Science, Technology and Innovation (MAARIFAH), King Abdulaziz City for Science and Technology, Kingdom of Saudi Arabia, Award Number (12– BIO2840–02). B.K. Cui thanked for the finance by the Fundamental Research Funds for the Central Universities (No. 2016ZCQ04) and the National Natural Science Foundation of China (Project No. 31422001). We would like to thank Dr. Marcela E. S. Cáceres for translating the German description of Clavulinopsis, the Conselho Nacional de Desenvolvimento Cientı́fico (CNPq) for the master scholarship of LSAN, the Pós- Fungal Diversity (2016) 80:1–270 Graduação em Biologia de Fungos (UFPE, Brazil), CNPq (Protax 562106/2010-3, Sisbiota 563342/2010-2, Universal 472792/2011-3) and FACEPE (APQ-0788-2.03/12) for financing this research. H.B. Lee was supported by the Graduate Program for the Undiscovered Taxa of Korea, and by the Project on Survey and Discovery of Indigenous Fungal Species of Korea, funded by NIBR and NNIBR of the Ministry of Environment (MOE), and in part by a fund from National Institute of Animal Science under Rural Development Administration, Republic of Korea. Aniket Ghosh, Priyanka Uniyal and R.P. Bhatt are grateful to the Head, Department of Botany & Microbiology, HNB Garhwal University, Srinagar Garhwal for providing all kinds of facilities during the present study. Kanad Das and Abhishek Baghela are thankful to the Director, Botanical Survey of India, Kolkata and Director, MACS’ Agharkar Research Institute, Pune respectively for providing facilities. UGC provided fellowship to Aniket Ghosh and Priyanka Unial. Field assistance rendered by Mr. Tahir Mehmood and Mr. Upendra Singh (HNBGU) are also duly acknowledged. Tuula Niskanen, Kare Liimatainen, Ilkka Kytövuori, Joe Ammirati, Bálint Dima, and Dimitar Bojantchev would like to acknowledge Heino Vänskä for the help with nomenclature. We are grateful to the curators of H and S. This work was partially supported by the Ministry of Environment, Finland (YM38/5512/2009) and Oskar Öflunds Stiftelse. The authors thanks Dr. Kerstin Voigt for the inestimable help in critical reviewing the lower fungi entries, the Coordenação de Aperfeiçoamento de Pessoal de Nı́vel Superior (CAPES) and Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco (FACEPE) for the postgraduate scholarships to Diogo X. Lima and Carlos A. F. de Souza, respectively. We also thank Conselho Nacional de Desenvolvimento Cientı́fico e Tecnológico (CNPq) and FACEPE for financial support through the projects: ‘Mucoromycotina in upland forests from the semi-arid of Pernambuco’ (CNPq—458391/2014-0), and ‘Diversity of Mucoromycotina in different ecosystems of the Pernambuco’s Atlantic Rainforest’ (FACEPE—APQ 0842-2.12/14). Z.L Luo and H.Y Su would like to thank the National Natural Science Foundation of China (Project ID: 31460015) for financial support on Study of the distribution pattern and driving factors of aquatic fungal diversity in the region of Three Parallel Rivers. C. Phukhamsakda would like to thank Dr. Matthew P. Nelsen for his valuable suggestions. Saranyaphat Boonmee thanks to the Thailand Research Fund, project number TRG5880152 and Mae Fah Luang University for a Grant Number 2559A30702006’’. C.G. Lin and Y. Wang thank for the finance by the National Natural Science Foundation of China (No. NSFC 31560489) and Fundamental Research on Science and Technology, Ministry of Science and Technology of China (2014FY120100). Haixia Wu would like to thank Dr. Shaun Pennycook for his kindly nomenclatural review and thanked for the finance by the National Natural Science Foundation of China (Project No. 31300019). S.C. Karunarathna, P.E. Mortimer and J.C. Xu would like to thank the World Agroforestry Centre, East and Central Asia Office; Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Science; the Chinese; Ministry of Science and Technology, under the 12th 5-year National Key Technology Support Program (NKTSP)2013 BAB07B06 integration and comprehensive demonstration of key technologies on Green Phosphate-mountaion Construction and the CGIAR Research Program 6: Forest, Trees and Agroforestry for partial funding. 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Fungal diversity notes 367–490: taxonomic and phylogenetic contributions to fungal taxa

Fungal diversity notes 367–490: taxonomic and phylogenetic contributions to fungal taxa

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