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“Induction of Turion formation by phosphate, nitrate and sulfate deficiency in the genus Wolffia” Hammad Ahmed Department of Microbiology, Friedrich Schiller University Jena, Germany 3.Study design 1. Introduction ➢Grow all species in nutrient (Phosphate and Nitrate) deficient medium. ➢Grow two species W. columbiana and W. arrhiza in Sulfate deficient medium. ➢Calculate Fresh weight and Dry weight content of floating and sinking (turions) plants. ➢Calculate Starch content of floating and sinking (turions) in all species of Genus Wolffia. Duckweed, or water lens, are flowering water plants that float on or just below the surface of stagnant or slow moving bodies of fresh water and wetlands. In the 20th century they are classified as a separate family of Lemnaceae (1). 4. Results ➢Among 11 species of genus Wolffia all show turion growth in nutrient (Phosphate, Nitrate and Sulfate) deficient medium . ➢There is no significant difference in Fresh weight and Dry wieght content of floating and sinking (turion) plants. ➢As per hypothesis that the starch content of turions are high as compare to floating but the investigation shows no significant difference between starch content of turions and floating plants. These plants are so basic, without a clear stem or leaves. The largest part of all plant is a tiny arranged structure of "thallus" or "frond" that has only a small number of cells in thickness, often with air pockets (aerenchyma) that allows Geit to float on or just below the surface of the plant. Water. Depending on the species, each plant may not have a root or have one or more simple roots (2). The propagation is generally by asexual budding, which occurs from a meristem enclosed in the base of the frond. Occasionally three small "flowers" are produced, consisting of two stamens and a pistil, through which sexual reproduction takes place. The flower of the genus of duckweed Wolffia is the smallest one, measuring only 0.3 mm long. Genus Wolffia The smallest flowering plant in the Plant Kingdom is Wolffia, commonly known as Watermeal, one of the Duckweeds, in the family Lemnaceae. It generally floats on the surface of the water. Each tiny Wolffia plant is a rootless oval body, producing a pistil (stigma) and a single stamen from a floral cavity in the side of the body. Wolffia includes 11 species that are Wolffia angusta, Wolffia arrhiza, Wolffia australiana, Wolffia borealis, Wolffia brasiliensis, Wolffia columbiana, Wolffia cylindracea, Wolffia elongata, Wolffia globosa, Wolffia microscopica, Wolffia neglecta (3). Formation of turions, or other vegetative propagules, is a common strategy amongst hydrophytes to survive in unfavorable conditions or to colonize new habitats. Turions of duckweeds are widely used for assessing morphogenesis, the dormant state, and the activation of vegetative buds (4). Mature turions have a higher density than water and, therefore, sink to the bottom after separation from the mother frond (5). 2. Aims To investigate the starch content of Turions formed by Genus Wolffia in deficiency of Phosphate, Nitrate and Sulfate. W. Arrhiza 9528 Fresh weight Floating (g) Fresh Weight Sinking (g) Dry Weight Floating (g) Dry Weight Sinking (g) Sinking % Fresh Sinking % Dry weight (%) weight (%) Starch Content Floating (%) Starch Content Sinking (%) -Phosphate 3.65 (±) 0.05 2.11 (±) 0.07 0.72 (±) 0.01 0.56 (±) 0.03 36.64% (±) 1.11% 43.66% (±) 1.11% 0.61% (±) 0.01% 0.63% (±) 0.04% -Nitrate 4.08 (±) 0.08 2.09 (±) 0.02 1.04 (±) 0.07 0.54 (±) 0.00 33.9% (±) 0.21% 34.44% (±) 1.54% 0.71% (±) 0.05 0.65 (±) 0.01 -Sulfate 1.97 (±) 0.04 0.91 (±) 0.01 0.54 (±) 0.12 0.2 (±) 0.02 31.52% (±) 0.07% 27.22% (±) 1.9% 0.41% (±) 0.01 0.42% (±) 0.01 5. Conclusions ➢As per hypothesis the turion starch content thought to be high and would be the cause that turions sinks down but after investigating this hypothesis not work and the starch content of floating plants and turions did not show any significant difference. ➢Similarly in fresh and dry weight content of floating and turions (sinking) plants did not show any significant difference. ➢While the overall plant growth in a medium shows 2/3 of floating plant weight and 1/3 of sinking plant weight. This is because floating plant grows faster and turions are always formed after the mature growth of floating plants. 6. References 1. Sheh-May Tam; Peter C. Boyce; Tim M. Upson; Denis Barabé; Anne Bruneau; Felix Forest; John S. Parker (2004), "Intergeneric and infrafamilial phylogeny of subfamily Monsteroideae (Araceae) revealed by chloroplast <011>trnL-F sequences", American Journal of Botany, 91: 490–498. 2. Sculthorpe, Cyril Duncan (1985). The biology of aquatic vascular plants. Koeltz Scientific Books. 3. Moar, N. T. (1990). "Ruth Mason, M.Sc. 1913–1990". New Zealand Journal of Botany. 28 (4). 4. Appenroth.Co-action of temperature and phosphate in inducing turion formation in Spirodela polyrhiza (great duckweed)Plant, Cell Environ., 25 (2002), pp. 1079-1085 5. Santamaría. Why are most aquatic plants widely distributed? Dispersal, clonal growth and small-scale heterogeneity in a stressful environmentActa Oecol., 23 (2002), pp. 137-154 7. Acknowledgements. Dr. Klaus. J. Appenroth Department of Plant Physiology, Friedrich Schiller University Jena, Germany