Persoonia 36, 2016: 316 – 458
www.ingentaconnect.com/content/nhn/pimj
RESEARCH ARTICLE
http://dx.doi.org/10.3767/003158516X692185
Fungal Planet description sheets: 400–468
P.W. Crous1,2, M.J. Wingfield 3, D.M. Richardson 4, J.J. Le Roux 4, D. Strasberg5,
J. Edwards 6, F. Roets7, V. Hubka8, P.W.J. Taylor 9, M. Heykoop10, M.P. Martín11,
G. Moreno10, D.A. Sutton12, N.P. Wiederhold12, C.W. Barnes13, J.R. Carlavilla10,
J. Gené14, A. Giraldo1,2, V. Guarnaccia1, J. Guarro14, M. Hernández-Restrepo1,2,
M. Kolařík15, J.L. Manjón10, I.G. Pascoe 6, E.S. Popov16, M. Sandoval-Denis14,
J.H.C. Woudenberg1, K. Acharya17, A.V. Alexandrova18, P. Alvarado19, R.N. Barbosa 20,
I.G. Baseia 21, R.A. Blanchette 22, T. Boekhout 3, T.I. Burgess 23, J.F. Cano-Lira14,
A. Čmoková 8, R.A. Dimitrov 24, M.Yu. Dyakov18, M. Dueñas11, A.K. Dutta17, F. EsteveRaventós10, A.G. Fedosova16, J. Fournier 25, P. Gamboa 26, D.E. Gouliamova 27,
T. Grebenc 28, M. Groenewald1, B. Hanse 29, G.E.St.J. Hardy 23, B.W. Held 22, Ž. Jurjević 30,
T. Kaewgrajang 31, K.P.D. Latha 32, L. Lombard1, J.J. Luangsa-ard 33, P. Lysková 34,
N. Mallátová 35, P. Manimohan 32, A.N. Miller 36, M. Mirabolfathy 37, O.V. Morozova 16,
M. Obodai 38, N.T. Oliveira20, M.E. Ordóñez 39, E.C. Otto22, S. Paloi17, S.W. Peterson 40,
C. Phosri 41, J. Roux3, W.A. Salazar 39, A. Sánchez10, G.A. Sarria42, H.-D. Shin43, B.D.B.
Silva21, G.A. Silva 20, M.Th. Smith1, C.M. Souza-Motta 44, A.M. Stchigel14,
M.M. Stoilova-Disheva 27, M.A. Sulzbacher 45, M.T. Telleria11, C. Toapanta 46,
J.M. Traba 47, N. Valenzuela-Lopez14,48, R. Watling 49, J.Z. Groenewald1
Key words
ITS DNA barcodes
LSU
novel fungal species
systematics
Abstract Novel species of fungi described in the present study include the following from Australia: Vermiculariopsi
ella eucalypti, Mulderomyces natalis (incl. Mulderomyces gen. nov.), Fusicladium paraamoenum, Neotrimmatostroma
paraexcentricum, and Pseudophloeospora eucalyptorum on leaves of Eucalyptus spp., Anungitea grevilleae (on
leaves of Grevillea sp.), Pyrenochaeta acaciae (on leaves of Acacia sp.), and Brunneocarpos banksiae (incl. Brun
neocarpos gen. nov.) on cones of Banksia attenuata. Novel foliicolous taxa from South Africa include Neosulcati
spora strelitziae (on Strelitzia nicolai), Colletotrichum ledebouriae (on Ledebouria floridunda), Cylindrosympodioides
brabejum (incl. Cylindrosympodioides gen. nov.) on Brabejum stellatifolium, Sclerostagonospora ericae (on Erica
sp.), Setophoma cyperi (on Cyperus sphaerocephala), and Phaeosphaeria breonadiae (on Breonadia microcephala).
Novelties described from Robben Island (South Africa) include Wojnowiciella cissampeli and Diaporthe cissampeli
(both on Cissampelos capensis), Phaeotheca salicorniae (on Salicornia meyeriana), Paracylindrocarpon aloicola
(incl. Paracylindrocarpon gen. nov.) on Aloe sp., and Libertasomyces myopori (incl. Libertasomyces gen. nov.)
on Myoporum serratum. Several novelties are recorded from La Réunion (France), namely Phaeosphaeriopsis
agapanthi (on Agapanthus sp.), Roussoella solani (on Solanum mauritianum), Vermiculariopsiella acaciae (on
Acacia heterophylla), Dothiorella acacicola (on Acacia mearnsii ), Chalara clidemiae (on Clidemia hirta), Cytospora
tibouchinae (on Tibouchina semidecandra), Diaporthe ocoteae (on Ocotea obtusata), Castanediella eucalypticola,
Phaeophleospora eucalypticola and Fusicladium eucalypticola (on Eucalyptus robusta), Lareunionomyces syzygii
(incl. Lareunionomyces gen. nov.) and Parawiesneriomyces syzygii (incl. Parawiesneriomyces gen. nov.) on leaves
of Syzygium jambos. Novel taxa from the USA include Meristemomyces arctostaphylos (on Arctostaphylos patula),
Ochroconis dracaenae (on Dracaena reflexa), Rasamsonia columbiensis (air of a hotel conference room), Paecilo
myces tabacinus (on Nicotiana tabacum), Toxicocladosporium hominis (from human broncoalveolar lavage fluid),
Nothophoma macrospora (from respiratory secretion of a patient with pneumonia), and Penidiellopsis radicularis
(incl. Penidiellopsis gen. nov.) from a human nail. Novel taxa described from Malaysia include Prosopidicola albiz
ziae (on Albizzia falcataria), Proxipyricularia asari (on Asarum sp.), Diaporthe passifloricola (on Passiflora foetida),
Paramycoleptodiscus albizziae (incl. Paramycoleptodiscus gen. nov.) on Albizzia falcataria, and Malaysiasca phaii
(incl. Malaysiasca gen. nov.) on Phaius reflexipetalus. Two species are newly described from human patients in
the Czech Republic, namely Microascus longicollis (from toenails of patient with suspected onychomycosis), and
Chrysosporium echinulatum (from sole skin of patient). Furthermore, Alternaria quercicola is described on leaves of
Quercus brantii (Iran), Stemphylium beticola on leaves of Beta vulgaris (The Netherlands), Scleroderma capever
deanum on soil (Cape Verde Islands), Scleroderma dunensis on soil, and Blastobotrys meliponae from bee honey
(Brazil), Ganoderma mbrekobenum on angiosperms (Ghana), Geoglossum raitviirii and Entoloma krutiсianum on
soil (Russia), Priceomyces vitoshaensis on Pterostichus melas (Carabidae) (Bulgaria) is the only one for which the
family is listed, Ganoderma ecuadoriense on decaying wood (Ecuador), Thyrostroma cornicola on Cornus officinalis
(Korea), Cercophora vinosa on decorticated branch of Salix sp. (France), Coprinus pinetorum, Coprinus littoralis and
Xerocomellus poederi on soil (Spain). Two new genera from Colombia include Helminthosporiella and Uwemyces
on leaves of Elaeis oleifera. Two species are described from India, namely Russula intervenosa (ectomycorrhizal
with Shorea robusta), and Crinipellis odorata (on bark of Mytragyna parviflora). Novelties from Thailand include
Cyphellophora gamsii (on leaf litter), Pisolithus aureosericeus and Corynascus citrinus (on soil). Two species are
newly described from Citrus in Italy, namely Dendryphiella paravinosa on Citrus sinensis, and Ramularia citricola on
Citrus floridana. Morphological and culture characteristics along with ITS nrDNA barcodes are provided for all taxa.
Article info Received: 1 March 2016; Accepted: 15 May 2016; Published: 4 July 2016.
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
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317
Fungal Planet description sheets
Acknowledgements Financial support was provided to María P. Martín,
Margarita Dueñas and M. Teresa Telleria by Plan Nacional I+D+I projects
No. CGL2009-07231 and CGL2012-35559. They also acknowledge Marian Glenn (Seton Hall University, USA) for her revision of the English text.
Anna G. Fedosova and Eugene S. Popov acknowledge financial support
from the Russian Foundation for Basic Research (project 15-29-02622).
Gabriel Moreno, Juan Ramón Carlavilla, Michel Heykoop and José Luis
Manjón wish to express their gratitude to Miguel Martin Calvo (Sociedad
Micológica de Madrid) and Manuel Castro-Marcote for sending them fungal
collections; to Dr L. Monje and Mr A. Pueblas of the Department of Drawing and Scientific Photography at the University of Alcalá for their help in
the digital preparation of the photographs; to Dr J. Rejos, curator of the AH
herbarium, for his assistance with the specimens examined in the present
study. Olga V. Morozova and Eugene S. Popov are grateful to the Russian
Foundation for Basic Research (project 15-04-04645a) for financial support.
Alina V. Alexandrova and Maxim Yu. Dyakov acknowledge financial support
from the Russian Science Foundation (project N 14-50-00029). K.P.D. Latha
acknowledges support from the Kerala State Council for Science, Technology and Environment (KSCSTE) in the form of a PhD fellowship (Grant No.
001/FSHP/2011/CSTE) and is thankful to the Principal Chief Conservator of
forests, Kerala State, for granting permission (No. WL10- 4937/2012, dated
03-10-2013) to collect agarics from the forests of Kerala. The research of
Vit Hubka, Miroslav Kolařík and Adéla Čmoková was supported through a
grant from the Charles University Grant Agency (GAUK 8615) and by the
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CBS-KNAW Fungal Biodiversity Centre, P.O. Box 85167, 3508 AD Utrecht,
The Netherlands; corresponding author e-mail: p.crous@cbs.knaw.nl.
Department of Microbiology and Plant Pathology, Forestry and Agricultural
Biotechnology Institute (FABI), University of Pretoria, P. Bag X20, Pretoria
0028, South Africa.
Forestry and Agricultural Biotechnology Institute (FABI), University of
Pretoria, Pretoria 0002, South Africa.
Centre for Invasion Biology, Department of Botany & Zoology, Stellenbosch
University, Matieland 7602, South Africa.
Université de La Réunion, UMR PVBMT, Peuplements Végétaux et Bioagresseurs en Milieu Tropical, 15 avenue René Cassin, CS 93002, 97 744
Saint-Denis Messag. Cedex 9, La Réunion, France.
AgriBio, Centre for AgriBiosciences, Department of Economic Development, Jobs, Transport and Resources, 5 Ring Road, La Trobe University,
Bundoora, Victoria 3083, Australia.
Department of Conservation Ecology and Entomology, Stellenbosch University, South Africa.
Department of Botany, Faculty of Science, Charles University in Prague,
Benátská 2, 12801 Prague 2, Czech Republic.
Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Australia.
Departamento de Ciencias de la Vida (Área de Botánica), Universidad de
Alcalá, E-28805 Alcalá de Henares, Madrid, Spain.
Departamento de Micología, Real Jardín Botánico-CSIC, Plaza de Murillo
2, 28014 Madrid, Spain.
Fungus Testing Laboratory, Department of Pathology, University of Texas
Health Science Center, 7703 Floyd Curl Dr., San Antonio, Texas 782293900, USA.
Departamento Nacional de Protección Vegetal, Estación Experimental
Santa Catalina, Instituto Nacional de Investigaciones Agropecuarias, Panamericana Sur Km. 1 vía Tambillo, Cantón Mejía, Provincia de Pichincha,
Quito, Ecuador.
Mycology Unit, Medical School and IISPV, Universitat Rovira i Virgili (URV),
Sant Llorenç 21, 43201 Reus, Tarragona, Spain.
Laboratory of Fungal Genetics and Metabolism, Institute of Microbiology
of the AS CR, v.v.i, Vídeňská 1083, 142 20 Prague 4, Czech Republic.
Laboratory of Systematics and Geography of Fungi, Komarov Botanical
Institute of the Russian Academy of Sciences, 197376, 2 Prof. Popov Str.,
Saint Petersburg, Russia.
Molecular and Applied Mycology and Plant Pathology Laboratory, Department of Botany, University of Calcutta, 35, Ballygunge Circular Road,
Kolkata-700019, West Bengal, India.
Lomonosov Moscow State University (MSU), Faculty of Biology, 119234,
1, 12 Leninskie Gory Str., Moscow, Russia.
ALVALAB, La Rochela n° 47, E-39012, Santander, Spain.
Departamento de Micologia Prof. Chaves Batista, Universidade Federal
de Pernambuco, Recife, Brazil.
Departamento de Botânica e Zoologia, Universidade Federal do Rio
Grande do Norte, Natal, Rio Grande do Norte, Brazil.
Department of Plant Pathology, University of Minnesota, 495 Borlaug Hall,
1991 Upper Buford Circle, St. Paul, MN 55108, USA.
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
project ‘BIOCEV–Biotechnology and Biomedicine Centre of the Academy
of Sciences and Charles University’ (CZ.1.05/1.1.00/02.0109) from the
European Regional Development Fund. Dilnora E. Gouliamova, Margarita
M. Stoilova-Disheva and Roumen A. Dimitrov acknowledge support from the
Bulgarian Science Fund (D002-TK-176) and EU FP6 Research and Infrastructure Synthesis grant. They are also grateful to Dr Borislav Guéorguiev
for the identification of insects. Margarita Hernández-Restrepo and Pedro
W. Crous thank Prof. Uwe Braun (Martin-Luther Univ. Halle, Germany) for
his comments on the fungi described as Helminthosporiella and Uwemyces.
Mention of trade names or commercial products in this publication is solely
for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is
an equal opportunity provider and employer. Johannes J. le Roux and David
M. Richardson acknowledge funding from the DST-NRF Centre of Excellence
for Invasion Biology. J. Jennifer Luangsa-ard, Alejandra Giraldo and Pedro
W. Crous have received funding from the European Union’s Horizon 2020
research innovation and staff exchange programme (RISE) under the Marie
Skłodowska-Curie grant agreement No. 645701. Francois Roets and Pedro
W. Crous thank the management board of Robben Island Museum, the Western Cape Nature Conservation board and Sabelo Madlala for permission to
undertake research on Robben Island. Josepa Gené acknowledges financial
support from the Spanish Ministerio de Economía y Competitividad grant
CGL 2011-27185. Maria E. Ordóñez and colleagues acknowledge financial
support from Secretaría de Educación Superior, Ciencia, Tecnología e Innovación del Ecuador (SENESCYT, Arca de Noé Initiative).
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Centre for Phytophthora Science and Management, Murdoch University,
90 South Street, Murdoch, WA 6150, Australia.
Sofia University “St. Kliment Ohridski”, 5 James Bourchier Blvd., Sofia
1164, Bulgaria.
Las Muros, 09420 Rimont, France.
Universidad Central del Ecuador, Facultad de Medicina, Carrera de
Ciencias Biológicas y Ambientales, Av. América, Quito, Ecuador.
The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of
Sciences, Acad. G. Bonchev 26, Sofia 1113, Bulgaria.
Slovenian Forestry Institute Vecna pot 2, Ljubljana, Slovenia.
IRS, P.O. Box 32, 4600 AA Bergen op Zoom, The Netherlands.
EMSL Analytical, Inc., 200 Route 130 North, Cinnaminson, NJ 08077,
USA.
Department of Forest Biology, Faculty of Forestry, Kasetsart University,
50 Ngamwongwan Rd, Latyao, Chatuchak, Bangkok 10900, Thailand.
Department of Botany, University of Calicut, Kerala, 673 635, India.
Microbe Interaction Laboratory, BIOTEC, Thailand Science Park, Khlong
Nueng, Klong Luang Pathum Thani 12120, Thailand.
Laboratory of Medical Mycology, Department of Parasitology, Mycology
and Mycobacteriology Prague, Public Health Institute in Usti nad Labem,
Sokolovská 60, 186 00 Prague 8, Czech Republic.
Laboratory of Mycology and Parasitology, Hospital České Budějovice, B.
Němcové 585/54, 370 01 České Budějovice, Czech Republic.
University of Illinois Urbana-Champaign, Illinois Natural History Survey,
1816 South Oak Street, Champaign, IL, 61820, USA.
Iranian Research Institute of Plant Protection, Tehran, Iran.
CSIR-Food Research Institute, P.O. Box M20, Accra, Ghana.
Escuela de Ciencias Biológicas, Pontificia Universidad Católica del Ecuador, Av. 12 de octubre 1076 y Roca, Quito, Ecuador.
Mycotoxin Prevention and Applied Microbiology Research Unit, Agricultural
Research Service, U.S. Department of Agriculture, 1815 North University
Street, Peoria, IL 61604, USA.
Faculty of Science, Nakhon Phanom University, 214, Moo 12, Nittayo
Road, Nong Yart Sub-district, Muang District, Nakhon Phanom, 48000,
Thailand.
Corporación Centro de Investigación en Palma de Aceite (CENIPALMA),
Colombia.
Division of Environmental Science and Ecological Engineering, Korea University, Seoul 02841, Korea.
URM Culture Collection, Recife, Brazil.
Departamento de Solos, Universidade Federal de Santa Maria, CCR,
Campus Universitário, 971050-900, Santa Maria, Rio Grande do Sul,
Brazil.
Escuela de Ciencias Biológicas, Pontificia Universidad Católica del Ecuador, Av. 12 de octubre 1076 y Roca, Quito, Ecuador.
Plaza de España 1, E 15001 A Coruña, Spain.
Microbiology Unit, Medical Technology Department, Faculty of Health
Science, University of Antofagasta, Av. Universidad de Antofagasta s/n,
02800 Antofagasta, Chile.
Caledonian Mycological Enterprises, Vrelah, 26 Blinkbonny Avenue, Edinburgh, EH4 3HU, Scotland, UK.
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Dothideomycetes
Tubeu
fiales
Botryosphaeriales
Venturiales
Acrosper
males
Pleosporales
J01355.1 Saccharomyces cerevisiae
EU019279.1 Phaeotheca triangularis
Incertae sedis
Fungal Planet 423 - Phaeotheca salicorniae
GU214469.1 Polychaeton citri
GU214400.1 Capnodium coffeae
Capnodiaceae
JN832615.1 Capnodium coartatum
EU981289.1 Phaeotheca fissurella
Incertae sedis
FJ790305.1 Toxicocladosporium rubrigenum
Fungal Planet 451 - Toxicocladosporium hominis
EU040243.2 Toxicocladosporium irritans
Cladosporiaceae
0.59 JX069858.1 Toxicocladosporium strelitziae
HQ599599.1 Toxicocladosporium banksiae
KJ869221.1 Acervuloseptoria ziziphicola
0.67
GQ852679.1 Sonderhenia eucalypticola
Fungal Planet 468 - Uwemyces elaeidis
Fungal Planet 415 - Phaeophleospora eucalypticola
KR476772.1 Phaeophleospora hymenocallidicola
KJ869188.1 Phaeophleospora parsoniae
Mycosphae
0.96
FJ493207.1 Phaeophleospora eugeniae
0.61
rellaceae
FJ493209.2 Phaeophleospora eugeniicola
AY490776.2 Ramularia endophylla
KP894124.1 Ramularia nyssicola
GU214691.1 Ramularia grevilleana
0.99
Fungal Planet 410 - Ramularia citricola
KF251725.1 Ramularia lamii
GU214429.1 Hortaea thailandica
0.88
Fungal Planet 412 - Meristemomyces arctostaphylos
0.92
GU250401.1 Meristemomyces frigidus
FJ493221.1 Teratosphaeria alboconidia
EU019296.1 Teratosphaeria zuluensis
GU323213.1 Teratosphaeria fibrillosa
Teratosphae
JF499862.1 Penidiella aggregata
0.54
riaceae
KC005792.1 Penidiella drakensbergensis
Fungal Planet 460 - Penidiellopsis radicularis
0.99
GQ852628.1 Phaeothecoidea intermedia
KF442565.1 Phaeothecoidea minutispora
0.69
Fungal Planet 432 - Neotrimmatostroma paraexcentricum
KF901840.1 Neotrimmatostroma excentricum
KP057802.1 Wiesneriomyces laurinus
KJ425450.1 Wiesneriomyces conjunctosporus
Wiesneriomycetaceae
Fungal Planet 435 - Parawiesneriomyces syzygii
0.91
KM052851.1 Pseudogliophragma indicum
DQ377914.1 Neofusicoccum andinum
0.81 KF766320.1 Neofusicoccum vitifusiforme
KT189496.1 Neofusicoccum parvum
0.64
0.99
JX556253.1 Neofusicoccum australe
0.90
KF766369.1 Neofusicoccum luteum
KR611900.1 Dothiorella ulmicola
Botryosphaeriaceae
JQ239416.1 Dothiorella brevicollis
0.94
KF766328.1 Dothiorella longicollis
0.99
JQ239418.1 Dothiorella pretoriensis
0.83
0.67
Fungal Planet 417 - Dothiorella acacicola
KF766391.1 Spencermartinsia rosulata
DQ377871.1 Spencermartinsia viticola
KX228353.1 Cylindrosympodium variabile
Venturiaceae s.lat.
EU0354141 Cylindrosympodium lauri
Fungal Planet 406 - Cylindrosympodioides brabejum
Incertae sedis
EU035463.1 Venturia maculiformis
EU035446.1 Protoventuria alpina
Venturiaceae s.str.
EU035461.1 Venturia lonicerae
0.93
JQ036233.1
Protoventuria major
0.99
Fungal Planet 425 - Fusicladium eucalypticola
HQ599601.1 Fusicladium eucalypti
Sympoventuriaceae
EU035424.1 Fusicladium africanum
EU035432.1 Fusicladium intermedium
EU035425.1 Fusicladium amoenum
Fungal Planet 429 - Fusicladium paraamoenum
2x
KF282652.1 Ochroconis constricta
AB564618.1 Ochroconis humicola
KT272083.1 Ochroconis musae
0.98
Fungal Planet 430 - Ochroconis dracaenae
EU940104.1 Acrospermum adeanum
Incertae sedis
KP004477.1 Arxiella dolichandrae
Fungal Planet 426 - Paramycoleptodiscus albizziae
JN711859.1 Mycoleptodiscus terrestris
AB807566.1 Periconia igniaria
Periconiaceae
EU552104.1 Pseudocamarosporium brabeji
Didymosphaeriaceae
Fungal Planet 407 - Dendryphiella paravinosa
0.91
KJ869196.1 Dendryphiella eucalyptorum
Dictyosporiaceae
0.99
0.94
EU848590.1 Dendryphiella vinosa
0.97 KJ474846.1 Roussoella thailandica
0.64
KJ474843.1 Roussoella nitidula
Fungal Planet 409 - Roussoella solani
Roussoellaceae
0.83
KJ474841.1 Roussoella neopustulans
0.98
KF443382.1 Roussoella intermedia
KF251654.1 Corynespora leucadendri
0.52
KP004478.1 Corynespora endiandrae
0.62
Fungal Planet 459 - Helminthosporiella stilbacea
0.98
FJ795449.1 Massarina eburnea
AB807522.1
Helminthosporium magnisporum
0.56
Massarinaceae
AB807521.1 Helminthosporium dalbergiae
0.63
AB807539.1 Massarina cisti
JQ044448.1 Corynespora olivacea
0.96
AB807530.1 Helminthosporium velutinum
0.94
GU205217.1 Byssothecium circinans
Capnodiales
Persoonia – Volume 36, 2016
0.1
Overview Dothideomycetes phylogeny
Consensus phylogram (50 % majority rule) of 42 902 trees resulting from a Bayesian analysis of the LSU sequence alignment (173 taxa including outgroup;
743 aligned positions; 375 unique site patterns) using MrBayes v. 3.2.5 (Ronquist et al. 2012). Bayesian posterior probabilities (PP) are shown at the nodes
and thickened lines represent nodes with PP = 1.00. The scale bar represents the expected changes per site. Families, orders and classes are indicated with
coloured blocks to the right of the tree. GenBank accession numbers are indicated in front of the species names. The tree was rooted to Saccharomyces
cerevisiae (GenBank J01355.1) and the novel species described in this study for which LSU sequence data were available are indicated in bold face. The
alignment and tree were deposited in TreeBASE (Submission ID 19280).
319
Fungal Planet description sheets
0.1
Overview Dothideomycetes phylogeny (cont.)
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
Incertae sedis
Cucurbitariaceae
Incertae sedis
Incertae sedis
Pleosporaceae
Coniothyriaceae
Didymellaceae
Coniothyriaceae
Phaeosphaeriaceae
Dothideomycetes (continued)
Incertae sedis
Pleosporales (continued)
GQ387620.1 Phialophorophoma litoralis
EU754206.1 Pyrenochaeta nobilis
KP120927.1 Camarosporium arezzoensis
KP744478.1 Camarosporium aureum
KR476754.1 Neoplatysporoides aloicola
DQ377883.1 Camarosporium quaternatum
Fungal Planet 428 - Libertasomyces myopori
KF251653.1 Coniothyrium sidae
KJ869209.1 Pyrenochaeta pinicola
0.79
Fungal Planet 413 - Pyrenochaeta acaciae
0.94
JQ044453.1 Pyrenochaeta protearum
AY849955.1 Pleospora halophila
0.74
KC584345.1 Pleospora tarda
Fungal Planet 442 - Stemphylium beticola
KJ790251.1 Pleospora herbarum
0.80
KP124545.1 Alternaria alternata
0.79 0.89
KC584319.1 Alternaria slovaca
KC584266.1 Alternaria conjuncta
Fungal Planet 441 - Alternaria quercicola
KP711384.1 Alternaria cesenica
KF251712.1 Coniothyrium carteri
EU167561.1 Pleiochaeta ghindensis
0.93
EU167563.1 Pleiochaeta setosa
Fungal Planet 400 - Thyrostroma cornicola
KX228302.1 Thyrostroma compactum
KX228301.1 Thyrostroma compactum
GU253716.1 Phaeomycocentrospora cantuariensis
0.58
GU238152.1 Ascochyta versabilis
GU238139.1 Didymella rhei
0.69
GU237999.1 Didymella aurea
0.94
GU238086.1 Xenodidymella humicola
KT389766.1 Xenodidymella asphodeli
EU754133.1 Ascochyta fabae
GU238037.1 Didymella aliena
KP330444.1 Ascochyta pisi
0.56 GU238116.1 Didymella negriana
GU237993.1 Didymella anserina
GU238079.1 Nothophoma gossypiicola
0.94
Fungal Planet 456 - Nothophoma macrospora
GU238039.1 Nothophoma anigozanthi
0.98
GU238089.1 Nothophoma infossa
GU238043.1 Nothophoma arachidishypogaeae
GQ387598.1 Coniothyrium glycines
KT950867.1 Neosulcatispora agaves
0.91
Fungal Planet 403 - Neosulcatispora strelitziae
0.90
GQ387591.1 Phaeosphaeria oryzae
KP004480.1 Phaeosphaeria podocarpi
0.93
KM434276.1 Phaeosphaeria thysanolaenicola
0.78
KP744502.1 Phaeosphaeria musae
KM434278.1 Phaeosphaeria musae
KR873278.1 Septoriella hirta
0.60
Fungal Planet 420 - Wojnowiciella cissampeli
0.97
KP684151.1 Wojnowicia lonicerae
0.98
KP684149.1 Wojnowicia dactylidis
KR476774.1 Wojnowiciella eucalypti
0.87
KM434282.1 Ophiosphaerella agrostidis
0.96
KP690985.1 Ophiosphaerella korrae
0.98
KP690992.1 Ophiosphaerella herpotricha
0.99
KC848510.1 Ophiosphaerella narmari
0.96
Fungal Planet 433 - Setophoma cyperi
KF251751.1 Setophoma terrestris
0.85 0.62
KF251748.1 Setophoma sacchari
KJ869198.1 Setophoma vernoniae
EU754218.1 Stagonospora neglecta var. colorata
KF366382.1 Scolicosporium minkeviciusii
KF251667.1 Neostagonospora caricis
0.82 Fungal Planet 416 - Sclerostagonospora ericae
DQ286772.1 Sclerostagonospora opuntiae
0.96
KP271444.1 Phaeosphaeria pleurospora
Fungal Planet 440 - Phaeosphaeria breonadiae
KT383798.1 Didymocyrtis aff. consimilis
KF251682.1 Parastagonospora poae
0.56
JX681115.1 Parastagonospora nodorum
0.94
0.83 KT383800.1 Didymocyrtis melanelixiae
0.71
KJ522480.1 Phaeosphaeriopsis triseptata
0.94
Fungal Planet 408 - Phaeosphaeriopsis agapanthi
0.51 JX681119.1 Phaeosphaeriopsis obtusispora
0.93
GQ387592.1 Phaeosphaeriopsis glaucopunctata
320
Consensus phylogram (50 % majority rule) of 4 352 trees resulting from a
Bayesian analysis of the LSU sequence alignment (42 taxa including outgroup; 769 aligned positions; 421 unique site patterns) using MrBayes v. 3.2.5
(Ronquist et al. 2012). Bayesian posterior probabilities (PP) are shown at the
nodes and thickened lines represent nodes with PP = 1.00. The scale bar
represents the expected changes per site. Families, orders and classes are
indicated with coloured blocks to the right of the tree. GenBank accession
numbers are indicated in front of the species names. The tree was rooted
to Diaporthe eres (GenBank AF362565.1) and the novel species described
in this study for which LSU sequence data were available are indicated in
bold face. The alignment and tree were deposited in TreeBASE (Submission ID 19280).
Leotiomycetes
Geoglos
Ostro
sales
pales
Geoglosso Lecanoro
mycetes
mycetes
Helotiales
Mycoca
liciales
Eurotiomycetes
Chaeto
thyriales
Onygenales
Saccharomycetes
Eurotiales
Saccharomycetales
Polyporales
Agaricomycetes
Overview Saccharomycotina and Agaricomycotina phylogeny
Agaricales
AF362565.1 Diaporthe eres
AY442294.1 Blastobotrys attinorum
Trichomonascaceae
DQ442684.1 Blastobotrys proliferans
0.99
Fungal Planet 462 - Blastobotrys meliponae
U45743.1 Priceomyces carsonii
Debaryomycetaceae
U45770.1 Priceomyces haplophilus
Fungal Planet 455 - Priceomyces fermenticarens
U45740.1 Priceomyces melissophilus
Fungal Planet 455 - Priceomyces northwykensis
Fungal Planet 455 - Priceomyces vitoshaensis
Fungal Planet 449 - Ganoderma mbrekobenum
0.75 AY333807.1 Ganoderma australe
0.60
JX310803.1 Ganoderma tornatum
0.73
KF495009.1 Ganoderma applanatum
Ganodermataceae
AB368068.1 Ganoderma lucidum
KU220017.1 Ganoderma hoehnelianum
0.57
KU220025.1 Ganoderma williamsianum
0.77
Fungal Planet 461 - Ganoderma ecuadoriense
2x
AY207179.1 Coprinus comatus
Fungal Planet 453 - Coprinus littoralis
Agaricaceae
Fungal Planet 454 - Coprinus pinetorum
KU686908.1 Coprinus spadiceisporus
AY691891.1 Entoloma sinuatum
Fungal Planet 457 - Entoloma kruticianum
Entolomataceae
KC710133.1 Entoloma conferendum
GQ289204.1 Entoloma vezzenaense
0.92
GQ289150.1 Entoloma abortivum
0.99
DQ421997.1 Russula emetica
JF834489.1 Russula brunneola
Russulaceae
Fungal Planet 463 - Russula intervenosa
EU019938.1 Russula rostraticystidia
JQ924322.1 Xerocomellus cisalpinus
0.70
Fungal Planet 458 - Xerocomellus poederi
Boletaceae
0.99
KF030276.1 Xerocomellus chrysenteron
KF030275.1 Xerocomellus zelleri
AF336271.1 Scleroderma verrucosum
EU718149.1 Scleroderma areolatum
0.91
DQ644138.1 Scleroderma bovista
Sclerodermataceae
KU759903.1 Scleroderma nitidum
0.99
Fungal Planet 448 - Scleroderma dunensis
0.84
AF261533.1 Scleroderma columnare
0.99
Fungal Planet 447 - Scleroderma capeverdeanum
0.10
0.99
Russu
lales
Consensus phylogram (50 % majority rule) of 9 452 trees resulting from a
Bayesian analysis of the LSU sequence alignment (46 taxa including outgroup; 751 aligned positions; 348 unique site patterns) using MrBayes v. 3.2.5
(Ronquist et al. 2012). Bayesian posterior probabilities (PP) are shown at the
nodes and thickened lines represent nodes with PP = 1.00. The scale bar
represents the expected changes per site. Families, orders and classes are
indicated with coloured blocks to the right of the tree. GenBank accession
numbers are indicated in front of the species names. The tree was rooted
to Saccharomyces cerevisiae (GenBank J01355.1) and the novel species
described in this study for which LSU sequence data were available are
indicated in bold face. The alignment and tree were deposited in TreeBASE
(Submission ID 19280).
J01355.1 Saccharomyces cerevisiae
Fungal Planet 419 - Mulderomyces natalis
Incertae sedis
HQ639664.1 Reimnitzia santensis
Graphidaceae
KP004481.1 Phacidiella podocarpi
Incertae sedis
AY300864.1 Stictis radiata
Stictidaceae
Fungal
Planet
450
Geoglossum
raitviirii
0.99
JQ256438.1 Geoglossum umbratile
AY533015.1 Geoglossum glabrum
Geoglossaceae
0.51
JQ256437.1 Geoglossum simile
0.55
KC222137.1 Geoglossum difforme
GU727553.1 Cistella spicicola
Hyaloscyphaceae s.lat.
0.90
0.67
KP234354.1 Fontanospora eccentrica
Helotiaceae
KP234355.1 Fontanospora fusiramosa
0.99
Fungal Planet 434 - Lareunionomyces syzygii
Incertae sedis
HQ609476.1 Exochalara longissima
JN086685.1 Calycellina populina
Hyaloscyphaceae
s.lat.
0.84
JN086682.1 Urceolella crispula
FJ176252.1 Chalara fungorum
FJ176253.1 Chalara parvispora
0.92
0.95
Incertae sedis
FJ176254.1 Chalara crassipes
0.96
FJ176249.1 Chalara africana
Fungal Planet 418 - Chalara clidemiae
AY853379.1 Mycocalicium subtile
0.97
AY795996.1 Chaenothecopsis golubkovae
0.90
Fungal Planet 466 - Brunneocarpos banksiae
Mycocaliciaceae
0.93
AY796002.1 Mycocalicium sequoiae
0.99
KF157987.1 Chaenothecopsis montana
KC455263.1 Cyphellophora reptans
0.87
Fungal Planet 405 - Cyphellophora gamsii
Cyphello
0.59
phoraceae
JQ766471.1 Cyphellophora laciniata
KC455259.1 Cyphellophora europaea
0.98 AB232893.1 Coccidioides immitis
AB232901.1 Coccidioides posadasii
0.99
Onygenaceae
AB040693.1 Aphanoascus mephitalis
0.98
AB075347.1 Aphanoascus saturnoideus
0.98
AB075345.1 Aphanoascus durus
0.67
Fungal Planet 446 - Chrysosporium echinulatum
Fungal Planet 445 - Paecilomyces tabacinus
0.95
FJ358279.1 Byssochlamys nivea
0.94
Thermoascaceae
JF922032.1 Byssochlamys spectabilis
FJ345354.1 Paecilomyces variotii
AF033387.1 Rasamsonia emersonii
DQ010015.1 Rasamsonia emersonii
Trichocomaceae
0.89
KC150021.1 Rasamsonia argillacea
0.68
Fungal Planet 443 - Rasamsonia columbiensis
AF033386.1 Rasamsonia cylindrospora
0.10
Boletales
Overview Eurotiomycetes, Geoglossomycetes, Lecanoromycetes and
Leotiomycetes phylogeny
Persoonia – Volume 36, 2016
321
Fungal Planet description sheets
Hypocreales
Microascales
Glomerellales
Chaeto
sphaeriales
Diaporthales
Sordariomycetes
Sordariales
Magnaporthales
Xylariales
J01355.1 Saccharomyces cerevisiae
HQ599593.1 Pseudophloeospora eucalypti
Incertae sedis
Fungal Planet 436 - Pseudophloeospora eucalyptorum
0.53
EU035441.1 Polyscytalum fecundissimum
Incertae sedis
Fungal Planet 402 - Anungitea grevilleae
KJ869176.1 Anungitea eucalyptorum
0.93
DQ923534.1 Phlogicylindrium eucalypti
0.95
Phlogicylindriaceae
EU040223.1 Phlogicylindrium eucalyptorum
EU552101.1 Anthostomella proteae
JF440974.1 Collodiscula japonica
Xylariaceae
0.97
AY544648.1 Xylaria hypoxylon
0.98
KP858987.1 Castanediella couratarii
KP858988.1 Castanediella cagnizarii
0.68
KR476763.1 Castanediella acaciae
Incertae sedis
0.99
Fungal Planet 414 - Castanediella eucalypticola
KR476758.1 Castanediella eucalypti
Fungal Planet 437 - Proxipyricularia asari
KM484986.1 Proxipyricularia zingiberis
KM484990.1 Pseudopyricularia cyperi
KT950865.1 Pseudopyricularia hagahagae
KM484993.1 Pseudopyricularia kyllingae
Pyriculariaceae
KM484995.1 Pyricularia grisea
0.70 KM009151.1 Neopyricularia commelinicola
0.99
KM485042.1 Xenopyricularia zizaniicola
0.78 KM485028.1 Pyricularia oryzae
0.56
KM485032.1 Pyricularia pennisetigena
0.63 JX280738 Chaetomium umbonatum
JX280700 Chaetomium globosum
LK932706 Corynascus fumimontanus
LK932708 Corynascus sexualis
Chaetomiaceae
0.92
Fungal Planet 465 - Corynascus citrinus
FJ666364.1 Corynascus sepedonium
0.99
LK932704.1 Corynascus verrucosus
0.58
KX171943.1 Cercophora solaris
Fungal Planet 467 - Cercophora vinosa
0.98
AY587936.1 Cercophora areolata
JN673036.1 Cercophora aquatica
0.94
Lasiosphaeriaceae
AY436414.1 Lasiosphaeria rugulosa
0.67
0.94
AY436410.1 Lasiosphaeria glabrata
AF064643.1 Lasiosphaeria ovina
0.94
AY436415.1 Lasiosphaeria sorbina
AF408357.1 Diaporthe pustulata
Fungal Planet 438 - Diaporthe passifloricola
Fungal Planet 439 - Diaporthe ocoteae
0.86
KT207683.1 Diaporthe arecae
Diaporthaceae
AF362565.1 Diaporthe eres
0.66
KF777209.1 Diaporthe diospyricola
0.63
0.99 Fungal Planet 421 - Diaporthe cissampeli
JX069848.1 Diaporthe canthii
JQ926271.1 Melanconiella ellisii
AF408369.1 Melanconiella chrysostroma
Melanconidaceae
JQ926301.1 Melanconiella spodiaea
JQ862750.1 Cryphonectria decipiens
Cryphonectriaceae
JN940857.1 Cryphonectria parasitica
0.53
Fungal Planet 422 - Prosopidicola albizziae
Incertae sedis
KX228354.1 Prosopidicola mexicana
0.92
Fungal Planet 431 - Cytospora tibouchinae
EU552121.1 Cytospora pruinosa
0.73
0.97
KT459412.1 Cytospora tanaitica
Valsaceae
0.76
DQ923537.1 Cytospora diatrypelloidea
0.96
EU552120.1 Cytospora eucalypticola
EF063575.1 Dictyochaeta cylindrospora
Fungal Planet 401 - Vermiculariopsiella eucalypti
Chaeto
sphaeriaceae
Fungal Planet 411 - Vermiculariopsiella acaciae
KJ869186.1 Vermiculariopsiella dichapetali
Fungal Planet 427 - Malaysiasca phaii
Incertae sedis
GU180640.1 Monilochaetes guadalcanalensis
GU180639.1 Monilochaetes infuscans
0.55
Australiascaceae
HM237323.1 Australiasca queenslandica
0.67
HQ609480.1 Monilochaetes dimorphospora
0.54
DQ286222.1 Colletotrichum agaves
Fungal Planet 404 - Colletotrichum ledebouriae
Glomerellaceae
0.76 KR476780.1 Colletotrichum neosansevieriae
KF777203.1 Colletotrichum euphorbiae
0.68
JN940411.1 Colletotrichum brevisporum
Fungal Planet 444 - Microascus longicollis
LN850830.1 Microascus senegalensis
LN850832.1 Microascus trigonosporus
Microascaceae
LN850815.1 Microascus cinereus
0.86
LN850812.1 Microascus cirrosus
LN850817.1 Microascus gracilis
HQ231988.1 Acremonium alternatum
AY489730.1 Hydropisphaera peziza
AF373281.1 Nalanthamala squamicola
Bionectriaceae
GQ505994.1 Lasionectria mantuana
0.66
GQ506013.1 Verrucostoma freycinetiae
0.76
Fungal Planet 424 - Paracylindrocarpon aloicola
0.10
GU075862.1 Hydropisphaera erubescens
Overview Sordariomycetes phylogeny
Consensus phylogram (50 % majority rule) of 21 302 trees resulting from a Bayesian analysis of the LSU sequence alignment (88 taxa including outgroup;
740 aligned positions; 299 unique site patterns) using MrBayes v. 3.2.5 (Ronquist et al. 2012). Bayesian posterior probabilities (PP) are shown at the nodes
and thickened lines represent nodes with PP = 1.00. The scale bar represents the expected changes per site. Families, orders and classes are indicated with
coloured blocks to the right of the tree. GenBank accession numbers are indicated in front of the species names. The tree was rooted to Saccharomyces
cerevisiae (GenBank J01355.1) and the novel species described in this study for which LSU sequence data were available are indicated in bold face. The
alignment and tree were deposited in TreeBASE (Submission ID 19280).
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
322
Persoonia – Volume 36, 2016
Thyrostroma cornicola
323
Fungal Planet description sheets
Fungal Planet 400 – 4 July 2016
Thyrostroma cornicola Crous & H.D. Shin, sp. nov.
Etymology. Name refers to Cornus, the plant genus from which this fungus
was collected.
Classification — Incertae sedis, Pleosporales, Dothideomy
cetes.
Sporodochia dark brown, punctiform, to 300 µm diam. Stromata
immersed to superficial, brown, 100–150 µm diam. Conidio
phores brown, finely roughened, subcylindrical, 1–3-septate,
10–50 × 7–10 µm. Conidiogenous cells brown, subcylindrical,
finely roughened, 7–20 × 7–10 µm, proliferating percurrently
at apex. Conidia clavate, ellipsoid to fusoid, medium brown,
with (1–)3 transverse septa, and 0–3 oblique or longitudinal
septa, apex broadly obtuse, base truncate, 5 – 6 µm diam,
(25–)30–36(–40) × (12–)14–17(–26) µm.
Culture characteristics — Colonies covering dish after 2 wk
at 25 °C, with fluffy aerial mycelium. On MEA surface pale
mouse-grey to mouse-grey, reverse dark mouse-grey. On PDA
and OA surface mouse-grey, reverse dark mouse-grey.
Notes — The genus Thyrostroma is based on the description
of T. compactum (CBS 700.70, ITS, LSU sequences GenBank
KX228250.1, KX228302.1), the ITS of which is 99 % (539/542)
similar to the present collection. However, T. compactum is
associated with Thyrostroma canker of Ulmus spp. in Europe
and the USA (Ellis 1971), while the present collection is associated with leaf spots on Cornus officinalis in Korea. Conidia of
T. compactum are 28–64 × 18–25 µm, with 2–4 transverse,
and 1 to several, longitudinal to oblique septa (Ellis 1971), thus
with conidia appearing somewhat larger than those observed
in the present collection.
Although Thyrostroma was linked to Dothidotthia by Phillips
et al. (2008), this treatment shows that the type of the genus
clusters in the Pleosporales, suggesting that the asexual morph
of Dothidotthia is thyrostroma-like, but that the two genera are
not congeneric.
Typus. Korea, Incheon, Namdong-gu, Incheon Arboretum, N37°27'37.1"
E126°45'22.6", on leaves of Cornus officinalis (Cornaceae), 28 Oct. 2014,
P.W. Crous & H.D. Shin (holotype CBS H-22589, culture ex-type CPC 25427 =
CBS 141280; ITS sequence GenBank KX228248.1, LSU sequence GenBank
KX228300.1, tef1 sequence GenBank KX228372.1, MycoBank MB816999).
Colour illustrations. Symptomatic leaves of Cornus officinalis; sporodochia
on PNA, sporulation on PNA, conidiophores and conidia. Scale bars = 10
µm.
Pedro W. Crous & Johannes Z. Groenewald, CBS-KNAW Fungal Biodiversity Centre, P.O. Box 85167, 3508 AD Utrecht, The Netherlands;
e-mail: p.crous@cbs.knaw.nl & e.groenewald@cbs.knaw.nl
Hyeon-Dong Shin, Division of Environmental Science and Ecological Engineering, Korea University, Seoul 02841, Korea;
e-mail: hdshin@korea.ac.kr
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
324
Persoonia – Volume 36, 2016
Vermiculariopsiella eucalypti
325
Fungal Planet description sheets
Fungal Planet 401 – 4 July 2016
Vermiculariopsiella eucalypti Crous, Jacq. Edwards & P.W.J. Taylor, sp. nov.
Etymology. Name refers to Eucalyptus, the plant genus from which this
fungus was collected.
Classification — Chaetosphaeriaceae, Chaetosphaeriales,
Sordariomycetes.
Colonies sporulating profusely throughout on SNA. Setae
erect, brown, cylindrical, straight to flexuous, 120–220 × 4–5
µm, thick-walled, finely roughened, 8–15-septate, tapering towards apex, developing a head of lateral coiled to whip-like
branches (constricted at base where attached to setae), that
are brown, septate, tapering, containing coiled, septate lateral
branches that could again contain coiled, lateral, branched,
mostly aseptate branches. Conidiophores arranged in a whorl
around base of setae, pale brown, smooth, subcylindrical,
branched or not, 0–6-septate, containing conidiogenous cells
that are arranged laterally along its length or at times reduced
to conidiogenous cells, 20–50 × 3–5 µm. Conidiogenous cells
solitary, monophialidic, discrete, ampulliform to subulate, pale
brown, 15–25 × 3–5 µm, apex 1–1.5 µm diam, with minute
collarette (1–2 µm long), at times with percurrent proliferation
at apex. Conidia asymmetrical, fusoid to subfusoid or oblong,
attenuated, base bluntly rounded to somewhat inflated, aseptate, smooth, finely granular, (5–)7–9(–10) × (2–)2.5(–3) µm.
Culture characteristics — Colonies spreading, with sparse
aerial mycelium, and even, lobate margins, reaching 30 mm
diam after 2 wk at 25 °C. On MEA surface pale mouse-grey,
reverse dark mouse-grey. On PDA surface and reverse pale
mouse-grey. On OA surface mouse-grey.
Notes — The setose conidiomata with brown, branched setae
and basally arranged phialides with periclinal thickening are
typical characteristics of the genus Vermiculariopsiella. On ITS
V. eucalypti is 95 % (487/511) similar to V. pediculata (FMR
12187; GenBank HF678527.1), followed by V. dichapetali (Crous
et al. 2014a; CPC 22463; GenBank KJ869129.1; 488/555
(88 %)). Vermiculariopsiella pediculata has smaller conidiogenous cells (14–15 × 3–4 μm), and narrower conidia (5–9 × 2
μm) (Hèrnandez-Restrepo et al. 2012). The closest hits using a
megablast search of the LSU sequence were 96 % (792/827)
similar to Dictyochaeta cylindrospora (GenBank EF063575.1;
Chaetosphaeriaceae, Chaetosphaeriales), 96 % (777/813) to
Vermiculariopsiella dichapetali (GenBank KJ869186.1; incertae
sedis, Microascales), 94 % (780/827) to Pseudobotryis ter
restris (GenBank KF771875.1; incertae sedis) and 94 % (781/
830) to Barbatosphaeria fimbriata (GenBank KM492867.1; in
certae sedis).
Typus. australia, Victoria, Toolangi State Forest, S37°33'25.3" E145°
31'55.9", on leaves of Eucalyptus regnans (Myrtaceae), 9 Nov. 2014, P.W.
Crous, J. Edwards & P.W.J. Taylor (holotype CBS H-22590, culture ex-type
CPC 25525 = CBS 141281; ITS sequence GenBank KX228251.1, LSU
sequence GenBank KX228303.1, MycoBank MB817000).
Colour illustrations. Toolangi State Forest; conidiophores with setae on
PNA, conidiogenous cells and conidia. Scale bars = 10 µm.
Pedro W. Crous & Johannes Z. Groenewald, CBS-KNAW Fungal Biodiversity Centre, P.O. Box 85167, 3508 AD Utrecht, The Netherlands;
e-mail: p.crous@cbs.knaw.nl & e.groenewald@cbs.knaw.nl
Jacqueline Edwards, AgriBio, Centre for AgriBiosciences, Department of Economic Development, Jobs, Transport and Resources,
5 Ring Road, La Trobe University, Bundoora, Victoria 3083, Australia;
e-mail: jacky.edwards@ecodev.vic.gov.au
Paul W.J. Taylor, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Australia;
e-mail: paulwjt@unimelb.edu.au
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
326
Persoonia – Volume 36, 2016
Anungitea grevilleae
327
Fungal Planet description sheets
Fungal Planet 402 – 4 July 2016
Anungitea grevilleae Crous & Jacq. Edwards, sp. nov.
Etymology. Name refers to Grevillea, the plant genus from which this
fungus was collected.
Classification — Incertae sedis, Xylariales, Sordariomycetes.
Mycelium of pale brown, smooth, septate, branched, 1.5–2.5
µm hyphae. Setae intermingled among conidiophores, flexuous,
subcylindrical with taper to acutely rounded apices, multiseptate, brown, smooth, base bulbous, 4–5 µm diam, up to 500 µm
tall, 2.5–3.5 µm wide. Conidiophores erect, flexuous, dark brown,
thick-walled, 1–4-septate, 15–70 × 2.5–3.5 µm, with several
sympodial, flat-tipped apical loci, 1–1.5 µm diam, not thickened.
Ramoconidia giving rise to branched chains of cylindrical conidia, hyaline to pale brown, smooth, subcylindrical, 0–1-septate, 15–20 × 2–3 µm, with 1–3 flat-tipped apical scars, 1.5–2
µm diam. Conidia hyaline, rarely pale olivaceous, cylindrical,
0 –1-septate, guttulate, ends truncate, (10 –)13 –16(– 22) ×
(2–)2.5–3 µm.
Culture characteristics — Colonies reaching up to 40 mm
diam after 2 wk at 25 °C, with spreading, flat surface; margins
smooth, even, and sparse to moderate aerial mycelium. On
MEA surface umber with patches of ochreous, reverse chestnut.
On OA surface umber with patches of honey. On PDA surface
and reverse umber.
Notes — On ITS Anungitea grevilleae is 98 % (553/566)
similar to A. eucalyptorum (CPC 17207 = CBS 137967; GenBank KJ869118.1). Morphologically, the two species are distinct in that A. eucalyptorum has shorter ramoconidia (12–17
× 2–3 μm), and somewhat larger conidia ((10–)13–16(–22)
× (2–)2.5–3 µm). The most obvious difference lies in the dimorphic conidiophores observed in A. eucalyptorum, where
microconidiophores can be reduced to conidiogenous cells,
and macroconidiophores are up to 180 µm tall (Crous et al.
2014a).
Typus. australia, Victoria, Royal Botanic Gardens Cranbourne, S38°7'
49.6" E145°16'9", on leaves of Grevillea sp. (Proteaceae), 7 Nov. 2014, P.W.
Crous & J. Edwards (holotype CBS H-22591, culture ex-type CPC 25576 =
CBS 141282; ITS sequence GenBank KX228252.1, LSU sequence GenBank
KX228304.1, MycoBank MB817001).
Colour illustrations. Leaves of Grevillea sp.; conidiophores sporulating
on PNA, seta, conidiophores and conidia. Scale bars = 10 µm.
Pedro W. Crous & Johannes Z. Groenewald, CBS-KNAW Fungal Biodiversity Centre, P.O. Box 85167, 3508 AD Utrecht, The Netherlands;
e-mail: p.crous@cbs.knaw.nl & e.groenewald@cbs.knaw.nl
Jacqueline Edwards, AgriBio, Centre for AgriBiosciences, Department of Economic Development, Jobs, Transport and Resources,
5 Ring Road, La Trobe University, Bundoora, Victoria 3083, Australia;
e-mail: jacky.edwards@ecodev.vic.gov.au
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
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Persoonia – Volume 36, 2016
Neosulcatispora strelitziae
329
Fungal Planet description sheets
Fungal Planet 403 – 4 July 2016
Neosulcatispora strelitziae Crous & M.J. Wingf., sp. nov.
Etymology. Name refers to Strelitzia, the plant genus from which this
fungus was collected.
Classification — Phaeosphaeriaceae, Pleosporales, Dothi
deomycetes.
Leaf spots amphigenous, subcircular to irregular, grey-brown
with dark brown margin, 3–8 mm diam. Conidiomata erumpent,
globose, dark brown to black, to 350 µm diam with central ostiole (to 40 µm diam), exuding a pale olivaceous conidial mass;
wall of 3–4 layers of brown textura angularis. Conidiophores
reduced to conidiogenous cells, or with a supporting cell, lining
the inner cavity, hyaline, smooth, ampulliform, 4–6 × 3–4 µm,
phialidic, with periclinal thickening or tightly aggregated percurrent proliferations. Conidia solitary, golden-brown, smooth,
ellipsoid, 0 –1-septate, (4–)5–7(–8) × (2.5–)3 µm.
Culture characteristics — Colonies flat, spreading, with moderate aerial mycelium, and smooth lobate margins, reaching
50 mm diam after 2 wk at 25 °C. On MEA surface mouse-grey,
reverse greyish sepia. On OA surface honey with black conidiomata. On PDA surface and reverse pale mouse-grey.
Notes — The ITS, LSU and tub2 sequences of the present
collection are 410/470 (87 %), 835/837 (99 %) and 440/533
(83 %) similar to Neosulcatispora agaves (CBS 140661; KT950853.1, KT950867.1, KT950883.1, respectively), which was
recently described from leaves of Agave veracruz growing in
La Réunion (Crous et al. 2015b). Morphologically, N. strelitziae
differs from N. agaves in that its conidiophores are reduced to
conidiogenous cells, and its conidia are smooth, whereas they
are larger ((7–)9–11(–12) × (3.5–)4(–4.5) μm), and prominently
striate in N. agaves (Crous et al. 2015b).
Typus. south africa, Eastern Cape Province, Haga Haga, on leaves of
Strelitzia nicolai (Strelitziaceae), Dec. 2014, M.J. Wingfield (holotype CBS
H-22592, culture ex-type CPC 25657 = CBS 141283; ITS sequence GenBank KX228253.1, LSU sequence GenBank KX228305.1, tub2 sequence
GenBank KX228380.1, MycoBank MB817002).
Colour illustrations. Strelitzia nicolai plants; conidiomata sporulating on
PNA, conidiogenous cells and conidia. Scale bars = 10 µm.
Pedro W. Crous & Johannes Z. Groenewald, CBS-KNAW Fungal Biodiversity Centre, P.O. Box 85167, 3508 AD Utrecht, The Netherlands;
e-mail: p.crous@cbs.knaw.nl & e.groenewald@cbs.knaw.nl
Michael J. Wingfield, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria,
Pretoria 0002, South Africa; e-mail: mike.wingfield@up.ac.za
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
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Persoonia – Volume 36, 2016
Colletotrichum ledebouriae
331
Fungal Planet description sheets
Fungal Planet 404 – 4 July 2016
Colletotrichum ledebouriae Crous & M.J. Wingf., sp. nov.
Etymology. Name refers to Ledebouria, the plant genus from which this
fungus was collected.
Classification — Glomerellaceae, Glomerellales, Sordario
mycetes.
Leaf spots circular, amphigenous, pale brown with raised dark
brown border, 10–20 mm diam. Conidiomata (on pine needle
agar; PNA) acervular, to 350 µm diam, conidiophores and setae
on a cushion of pale brown stroma. Setae dark brown, smoothwalled, 2–4-septate, 80–120 × 5–7 µm, tapering to subacute
apex. Conidiophores hyaline to pale brown, smooth-walled,
septate, branched, to 50 µm tall, 4–5 µm wide. Conidiogenous
cells hyaline to pale brown, smooth-walled, 15–23 × 3.5–4.5
µm. Conidia hyaline, smooth-walled, guttulate, aseptate,
straight, subcylindrical, apex obtuse, base truncate with hilum
1–1.5 µm diam, (15 –)17– 21(–22) × (5–)6 µm.
Culture characteristics — Colonies covering dish after 1 mo
at 25 °C, with moderate to woolly aerial mycelium. On MEA
surface grey olivaceous, reverse dark brick. On OA surface
smoke grey. On PDA surface and reverse grey olivaceous.
Notes — Ledebouria is a genus of deciduous or weakly
evergreen bulb plants that occur in Sub-Saharan Africa, but
almost nothing is known regarding fungal diseases of these
plants (Crous et al. 2000). As far as we could establish, this is
the first record of anthracnose disease on Ledebouria. On ITS
C. ledebouriae is 98 % (563/572) similar to C. sansevieriae
(MAFF239721; GenBank KC790947.1). The most similar sequences based on actA and his3 are 94 % (242/257) and 93 %
(346/372) to C. neosansevieriae (GenBank KR476790.1 and
KR476792.1), and 90 % (230/255) and 92 % (343/371) to C. eu
phorbiae (GenBank KF777125.1 and KF777134.1). Conidia of
C. sansevieriae are larger (12.5–(18.4)–32.5 × 2.8–(6.4)–8.8
µm; Nakamura et al. 2006) than those of C. ledebouriae, but
overlap with those of C. neosansevieriae ((16–)18–22(–25) ×
(4–)5–6 μm; Crous et al. 2015a).
Typus. south africa, Eastern Cape Province, Haga Haga, on leaves of
Ledebouria floridunda (Hyacinthaceae), Dec. 2014, M.J. Wingfield (holotype
CBS H-22593, culture ex-type CPC 25671 = CBS 141284; ITS sequence
GenBank KX228254.1, LSU sequence GenBank KX228306.1, actA sequence GenBank KX228357.1, his3 sequence GenBank KX228365.1, MycoBank MB817003).
Colour illustrations. Coastline at Haga Haga in the Eastern Cape Province;
conidiomata sporulating on OA and PNA, setae and conidia. Scale bars =
10 µm.
Pedro W. Crous & Johannes Z. Groenewald, CBS-KNAW Fungal Biodiversity Centre, P.O. Box 85167, 3508 AD Utrecht, The Netherlands;
e-mail: p.crous@cbs.knaw.nl & e.groenewald@cbs.knaw.nl
Michael J. Wingfield, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria,
Pretoria 0002, South Africa; e-mail: mike.wingfield@up.ac.za
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
332
Persoonia – Volume 36, 2016
Cyphellophora gamsii
Fungal Planet description sheets
333
Fungal Planet 405 – 4 July 2016
Cyphellophora gamsii Crous, sp. nov.
Etymology. Named for Walter Gams, who collected and isolated this
fungus.
Classification — Cyphellophoraceae, Chaetothyriales, Eu
rotiomycetes.
Mycelium consisting of smooth, pale brown, branched, septate,
1.5–2 µm diam hyphae. Conidiophores reduced to conidiogenous cells on hyphae, erect, straight, cylindrical, 2–6 × 1.5–2
µm; apex with flared collarette, 1–2 µm long. Conidia solitary,
granular, hyaline, smooth, curved, falcate to flexuous, tapering from middle to subacutely rounded apex with mucoid cap,
and towards truncate hilum, 0.5 µm diam, (0 –)3-septate,
(22–)30–40(–50) × (1.5–)2 µm; microcyclic conidiation observed in culture.
Culture characteristics — Colonies reaching up to 20 mm
diam after 2 wk at 25 °C, with spreading, flat surface; margins
smooth, lobate, and moderate aerial mycelium. On MEA and
PDA surface mouse-grey, reverse dark mouse-grey. On OA
surface dark mouse-grey.
Notes — The genus Cyphellophora includes species that are
associated with plant litter, as well as human and animal skin
and nails (Gao et al. 2015). Cyphellophora gamsii is a typical
species in the genus, being 99 % (812/821) similar on LSU to
other known species. On ITS C. gamsii is 99 % (531/537) similar to Cyphellophora sp. (CBS 112.94; GenBank JQ766437.1)
and 95 % (582/615) to C. laciniata (CBS 190.61; GenBank
EU035416.1). No highly similar tub2 sequences were found.
Morphologically, C. gamsii is quite distinct from other species
in the genus by having large, 3-septate conidia (Decock et al.
2003, Gao et al. 2015).
Typus. thailand, Chiang Mai, Mushroom Research Centre, on leaf litter,
Aug. 2014, W. Gams (holotype MFLU 16-1139, culture ex-type CPC 25867;
ITS sequence GenBank KX228255.1, LSU sequence GenBank KX228307.1,
tub2 sequence GenBank KX228381.1, MycoBank MB817005).
Colour illustrations. Mountain stream at Chiang Mai; hyphae with fertile
conidiogenous loci and conidia on PNA. Scale bars = 10 µm.
Pedro W. Crous & Johannes Z. Groenewald, CBS-KNAW Fungal Biodiversity Centre, P.O. Box 85167, 3508 AD Utrecht, The Netherlands;
e-mail: p.crous@cbs.knaw.nl & e.groenewald@cbs.knaw.nl
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
334
Persoonia – Volume 36, 2016
Cylindrosympodioides brabejum
335
Fungal Planet description sheets
Fungal Planet 406 – 4 July 2016
Cylindrosympodioides Crous & M.J. Wingf., gen. nov.
Etymology. Name refers to the morphological similarity with the genus
Cylindrosympodium.
Classification — Incertae sedis, Venturiales, Dothideomy
cetes.
Mycelium consisting of smooth, pale brown, branched, septate,
hyphae. Conidiophores erect, medium brown, cylindrical, septate. Conidiogenous cells terminal, subcylindrical, pale brown,
proliferating sympodially, scars unthickened, slightly darkened, flat. Conidia solitary, hyaline, acicular, straight to slightly
curved, guttulate, multiseptate, apex subobtusely rounded,
base prominently truncate, unthickened but slightly darkened.
Fusicladium-like synasexual morph developing on SNA, intermixed on hyphae with Cylindrosympodioides morph. Conidio
phores reduced to conidiogenous cells, brown, ampulliform
to fusoid-ellipsoid, proliferating sympodially, scars somewhat
darkened. Conidia solitary, brown, verruculose, guttulate, thickwalled, fusoid-ellipsoid, septate, widest at median septum, apex
subobtusely rounded, base truncate.
Type species. Cylindrosympodioides brabejum Crous & M.J. Wingf.
MycoBank MB817076.
Cylindrosympodioides brabejum Crous & M.J. Wingf., sp. nov.
Etymology. Name refers to Brabejum, the plant genus from which this
fungus was collected.
Mycelium consisting of smooth, pale brown, branched, septate,
1.5–2 µm diam hyphae. Conidiophores erect, medium brown,
cylindrical, 0–2-septate, 10–25 × 2.5–3.5 µm. Conidiogenous
cells terminal, subcylindrical, pale brown, 7–15 × 2.5–3 µm,
proliferating sympodially, scars unthickened, slightly darkened,
flat, 1.5–2 µm diam. Conidia solitary, hyaline, acicular, straight
to slightly curved, guttulate, multiseptate, apex subobtusely
rounded, base prominently truncate, 2 µm diam, unthickened
but slightly darkened, (55–)100–110(–120) × (1.5–)2(–2.5) µm.
Fusicladium-like synasexual morph developing on SNA, intermixed on hyphae with Cylindrosympodioides morph. Conidio
phores reduced to conidiogenous cells, brown, ampulliform to
fusoid-ellipsoid, 5–7 × 3–4 µm, proliferating sympodially, scars
somewhat darkened, 0.5 µm diam. Conidia solitary, brown, verruculose, guttulate, thick-walled, fusoid-ellipsoid, 1(–3)-septate,
widest at median septum, apex subobtusely rounded, base
truncate, 1 µm diam, (12–)15–17(– 20) × (2.5–)3(–3.5) µm.
Culture characteristics — Colonies reaching up to 15 mm
diam after 2 wk at 25 °C, with spreading, flat surface; margins
smooth, lobate, and moderate aerial mycelium. On MEA surface
isabelline, reverse sepia. On OA surface dark brick. On PDA
surface isabelline, reverse sepia.
Notes — The genus Cylindrosympodium (based on C. varia
bile) is characterised by its solitary, septate, cylindrical to subacicular, hyaline conidia with truncate bases, somewhat darkened hila, and brown conidiogenous structures with sympodial
proliferation (Crous et al. 2007d). Cylindrosympodioides, which
shares a similar morphology with species of Cylindrosym
podium, is distinct in that it has acicular conidia with slightly
thickened hila, and a fusicladium-like synasexual morph, which
has conidiophores that are reduced to conidiogenous cells. Cy
lindrosympodioides is phylogenetically also closer to Venturia,
whereas Cylindrosympodium forms a distinct sister lineage
basal in the Venturiaceae.
Typus. south africa, Western Cape Province, Franschhoek, on leaves
of Brabejum stellatifolium (Proteaceae), 17 Jan. 2015, P.W. Crous & M.J.
Wingfield (holotype CBS H-22594, culture ex-type CPC 25934 = CBS 141285;
ITS sequence GenBank KX228256.1, LSU sequence GenBank KX228308.1,
MycoBank MB817008).
Colour illustrations. Symptomatic leaves of Brabejum stellatifolium; conidiogenous cells with Fusicladium conidia, colony on SNA, conidiophores
and conidia of Cylindrosympodioides morph. Scale bars = 10 µm.
Pedro W. Crous & Johannes Z. Groenewald, CBS-KNAW Fungal Biodiversity Centre, P.O. Box 85167, 3508 AD Utrecht, The Netherlands;
e-mail: p.crous@cbs.knaw.nl & e.groenewald@cbs.knaw.nl
Michael J. Wingfield, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria,
Pretoria 0002, South Africa; e-mail: mike.wingfield@up.ac.za
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
336
Persoonia – Volume 36, 2016
Dendryphiella paravinosa
337
Fungal Planet description sheets
Fungal Planet 407 – 4 July 2016
Dendryphiella paravinosa Crous & Guarnaccia, sp. nov.
Etymology. Name reflects the morphological similarity to Dendryphiella
vinosa.
Classification — Dictyosporiaceae, Pleosporales, Dothideo
mycetes.
Mycelium consisting of hyaline to pale brown, smooth to verruculose 2–3 µm diam hyphae. Conidiophores solitary, erect,
dark brown, subcylindrical, verruculose, branched above and
below, to 150 µm long, 10–20 × 6–7 µm, 5–7-septate. Conidiog
enous cells integrated, terminal and intercalary, clavate, with
several loci arranged at the apex, 2–3 µm diam; loci thickened,
darkened, refractive, 2–3 µm diam, with central pore. Conidia
subcylindrical, apex obtuse, base bluntly rounded, medium
brown, verruculose, (1–)3-septate, occurring in short chains
(10–)24–27(–33) × (6–)7(–7.5) µm; hila thickened, darkened,
refractive, 2–3 µm diam.
Culture characteristics — Colonies reaching up to 40 mm
diam after 2 wk at 25 °C, with spreading, flat surface; margins
smooth, lobate, and moderate aerial mycelium. On MEA surface
honey to ochreous, reverse umber. On OA surface chestnut.
On PDA surface umber to ochreous, reverse umber.
Notes — The genus Dendryphiella is characterised by
branched to unbranched conidiophores, with polytretic conidiogenous cells, darkened, thickened scars, and brown,
septate, catenulate conidia (Crous et al. 2014a). Conidia of
D. paravinosa resemble those of D. vinosa (13 – 39 × 4 – 8
μm; Ellis 1971, described from Congo bean in Cuba), but are
smaller. Dendryphiella paravinosa CPC 26176 = CBS 141286
is identical to CPC 26182, and they are 90 % (424/472) related
to D. vinosa (NBRC 32669; GenBank DQ307316.1), 88 %
(374/427) to Dictyosporium toruloides (FMR 11942; GenBank
HF677181.1) and 90 % (526/582) to Dendryphiella eucalypto
rum (CPC 22927 = CBS 137987; GenBank KJ869139.1).
Typus. italy, Sicily, Scordia (CT), on leaves of Citrus sinensis (Rutaceae),
Mar. 2015, V. Guarnaccia (holotype CBS H-22595, culture ex-type CPC
26176 = CBS 141286; ITS sequence GenBank KX228257.1, LSU sequence
GenBank KX228309.1, MycoBank MB817009); Sicily, Scordia (CT), on leaves
of Citrus limon, Mar. 2015, V. Guarnaccia, CPC 26182 (ITS sequence GenBank KX228258.1).
Colour illustrations. Citrus sinensis orchard; colony sporulating on PNA,
conidiophores and chains of conidia. Scale bars = 10 µm.
Pedro W. Crous, Johannes Z. Groenewald & Vadimiro Guarnaccia, CBS-KNAW Fungal Biodiversity Centre,
P.O. Box 85167, 3508 AD Utrecht, The Netherlands;
e-mail: p.crous@cbs.knaw.nl, e.groenewald@cbs.knaw.nl & v.guarnaccia@cbs.knaw.nl
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
338
Persoonia – Volume 36, 2016
Phaeosphaeriopsis agapanthi
339
Fungal Planet description sheets
Fungal Planet 408 – 4 July 2016
Phaeosphaeriopsis agapanthi Crous & M.J. Wingf., sp. nov.
Etymology. Name refers to Agapanthus, the host plant from which this
fungus was collected.
Classification — Phaeosphaeriaceae, Pleosporales, Dothi
deomycetes.
Conidiomata erumpent, globose, black, to 250 µm diam with
central ostiole; wall of 2–3 layers of black textura angularis.
Conidiophores reduced to conidiogenous cells lining the inner
cavity, ampulliform to doliiform, hyaline, phialidic, with periclinal
thickening, or percurrent proliferation, 5–7 × 5–6 µm. Conidia
solitary, aseptate, golden-brown, verruculose, subcylindrical,
apex obtuse, base bluntly rounded to truncate, (6–)7–8(–9)
× 3(–3.5) µm.
Culture characteristics — Colonies reaching up to 40 mm
diam after 2 wk at 25 °C, with spreading, flat surface; margins
smooth, lobate (feathery on PDA), and sparse to moderate
aerial mycelium. On MEA surface pale luteous, reverse luteous.
On OA and PDA surface and reverse pale luteous.
Notes — The genus Phaeosphaeriopsis (based on P. glauco
punctata) is characterised by immersed, globose to subglobose
to pyriform ascomata, cylindrical asci and septate, verrucose
ascospores with coniothyrium-like or Phaeostagonospora asexual morphs (Câmara et al. 2003). The genus was recently
revised by Thambugala et al. (2014), who accepted seven
species. The aseptate conidia of P. agapanthi suggest that
it should be compared to P. obtusispora, but, based on ITS,
phylogenetically it is closest 98 % (558/568) to P. triseptata
(MFLUCC 13-0347; GenBank KJ522476.1), and only 93 %
(525/563) to P. obtusispora (GenBank AF250822.1). Of the two
isolates studied, CPC 26301 is identical on its DNA sequence
(ITS) to CPC 26303.
Typus. france, La Réunion, S21°3'39.5" E55°32'10.6", on leaves of Aga
panthus precox (Amaryllidaceae), 8 Mar. 2015, P.W. Crous & M.J. Wingfield
(holotype CBS H-22596, culture ex-type CPC 26303 = CBS 141287; ITS
sequence GenBank KX228260.1, LSU sequence GenBank KX228311.1,
MycoBank MB817012); ibid., CPC 26301= CBS 141316 (ITS sequence
GenBank KX228259.1, LSU sequence GenBank KX228310.1).
Colour illustrations. Symptomatic leaves of Agapanthus precox; conidiomata sporulating on PNA and OA, conidiogenous cells and conidia. Scale
bars = 10 µm.
Pedro W. Crous, CBS-KNAW Fungal Biodiversity Centre, P.O. Box 85167, 3508 AD Utrecht, The Netherlands; e-mail: p.crous@cbs.knaw.nl
Michael J. Wingfield, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria,
Pretoria 0002, South Africa; e-mail: mike.wingfield@up.ac.za
Johannes J. Le Roux & David M. Richardson, Centre for Invasion Biology, Department of Botany & Zoology,
Stellenbosch University, Matieland 7602, South Africa;
e-mail: jleroux@sun.ac.za & rich@sun.ac.za
Dominique Strasberg, Université de La Réunion, UMR PVBMT, Peuplements Végétaux et Bioagresseurs en Milieu Tropical,
15 avenue René Cassin, CS 93002, 97 744 Saint-Denis Messag. Cedex 9, La Réunion, France; e-mail: dominique.strasberg@univ-reunion.fr
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
340
Persoonia – Volume 36, 2016
Roussoella solani
341
Fungal Planet description sheets
Fungal Planet 409 – 4 July 2016
Roussoella solani Crous & M.J. Wingf., sp. nov.
Etymology. Name refers to Solanum, the plant genus from which this
fungus was collected.
Classification — Roussoellaceae, Pleosporales, Dothideo
mycetes.
Conidiomata immersed to erumpent, solitary, globose, brown,
to 150 µm diam with central ostiole, exuding a grey-brown
conidial mass; wall of 3–4 layers of brown textura angularis.
Conidiophores reduced to conidiogenous cells lining the inner
cavity, hyaline, smooth, ampulliform to doliiform, 4–6 × 3–4
µm, phialides with visible periclinal thickening. Conidia solitary,
aseptate, smooth, pale brown, subcylindrical, apex obtuse,
base bluntly rounded to truncate, (4 –)4.5 – 5(–7) × 2(– 3) µm.
Culture characteristics — Colonies reaching up to 40 mm
diam after 2 wk at 25 °C, with spreading, erumpent surface;
margins smooth to feathery, lobate, and moderate aerial mycelium. On MEA surface pale luteous with patches of scarlet,
reverse luteous with patches of umber. On OA surface sienna
with patches of umber and scarlet. On PDA surface pale vinaceous with diffuse scarlet pigment in agar, reverse isabelline
in centre, scarlet at margin.
Notes — Based on the LSU sequence, Roussoella solani
is accommodated in the Roussoellaceae (e.g. 98 % (787/803)
similarity to R. thailandica GenBank KJ474846.1), but on ITS
data it is phylogenetically distinct from other known taxa within
the genus, showing less than 90 % similarity. Unfortunately,
R. solani is known only from its asexual morph and hence a
full morphological comparison with other species known in the
genus is presently not possible. Recent studies have shown that
several species of Roussoella occur on woody plants (Crous
et al. 2014b, 2015b), and are not only restricted to monocotyledons.
Typus. france, La Réunion, on stems of Solanum mauritianum (Solana
ceae), 13 Mar. 2015, P.W. Crous & M.J. Wingfield (holotype CBS H-22597,
culture ex-type CPC 26331 = CBS 141288; ITS sequence GenBank KX228261.1, LSU sequence GenBank KX228312.1, MycoBank MB817016).
Colour illustrations. Valley in La Réunion; conidiomata sporulating on
PNA, conidiogenous cells and conidia. Scale bars = 10 µm.
Pedro W. Crous, CBS-KNAW Fungal Biodiversity Centre, P.O. Box 85167, 3508 AD Utrecht, The Netherlands; e-mail: p.crous@cbs.knaw.nl
Michael J. Wingfield, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria,
Pretoria 0002, South Africa; e-mail: mike.wingfield@up.ac.za
Johannes J. Le Roux & David M. Richardson, Centre for Invasion Biology, Department of Botany & Zoology,
Stellenbosch University, Matieland 7602, South Africa; e-mail: jleroux@sun.ac.za & rich@sun.ac.za
Dominique Strasberg, Université de La Réunion, UMR PVBMT, Peuplements Végétaux et Bioagresseurs en Milieu Tropical,
15 avenue René Cassin, CS 93002, 97 744 Saint-Denis Messag. Cedex 9, La Réunion, France; e-mail: dominique.strasberg@univ-reunion.fr
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
342
Persoonia – Volume 36, 2016
Ramularia citricola
343
Fungal Planet description sheets
Fungal Planet 410 – 4 July 2016
Ramularia citricola Crous & Guarnaccia, sp. nov.
Etymology. Name refers to Citrus, the plant genus from which this fungus
was collected.
Classification — Mycosphaerellaceae, Capnodiales, Dothi
deomycetes.
Mycelium consisting of septate, branched, hyaline, smooth,
2–2.5 µm diam hyphae. Conidiophores solitary, arising from hyphae as lateral branches, or terminal in loose fascicles, straight
to geniculate-sinuous, erect, hyaline, smooth, subcylindrical,
reduced to conidiogenous loci on hyphae, or 0–1-septate, erect,
2–20 × 2–2.5 µm. Conidiogenous cells hyaline, smooth, subcylindrical, 2–16 × 2–2.5 µm; loci terminal, thickened, darkened
and refractive, 1 µm diam. Primary ramoconidia hyaline, smooth
to finely roughened, subcylindrical, 0–1-septate, 22–33 × 2.5–3
µm. Secondary ramoconidia subcylindrical, 0–1-septate, finely
roughened, guttulate, 8–22 × 2–2.5 µm. Intermediary conidia
subcylindrical-fusiform, 0(–1)-septate, 7–9 × 2 µm. Conidia in
branched chains, ellipsoid-fusoid, smooth to finely roughened,
(3–)6–8(–9) × 2 µm; loci thickened, darkened and refractive,
0.5 µm diam.
Culture characteristics — Colonies reaching up to 15 mm
diam after 2 wk at 25 °C, with spreading, erumpent, folded
surface; margins smooth, lobate, and sparse aerial mycelium.
On MEA surface luteous, reverse ochreous. On OA surface
mouse-grey. On PDA surface pale mouse-grey to mouse-grey,
reverse mouse-grey.
Notes — On ITS Ramularia citricola is 99 % (524/531)
similar to R. grevilleana (CPC 4903; GenBank GU214691.1)
and 98 % (523/531) R. grevilleana (isolate s208; GenBank
GU939181.1). None of the sequences from the protein coding genes resulted in similarities higher than 92 %. Ramularia
grevilleana can be distinguished from R. citricola by having
larger conidia that are ellipsoid-ovoid, subcylindrical-fusoid,
(8 –)15 – 45(– 55) × (1.5 –)2.5 – 4.5(– 5) µm, 0 – 2(– 3)-septate
(Braun 1998). Two species of Ramularia have been described
from Citrus, namely R. citri and R. citrifolia. Ramularia citri (on
Citrus aurantium, Italy) was described from fallen, dry leaves
(asymptomatic), with catenate conidia, oblong, 8–14 × 3.5–4
µm, 0–2-septate. Type material of the latter species could not
be traced, and its generic affinity remains unclear (Braun 1998).
Ramularia citrifolia (hyperparasitic on Meliola butleri on Citrus
tankan, Taiwan) was allocated to Eriomycopsis by Braun (1993).
Typus. italy, Sicily, Messina, on twigs of Citrus floridana (Rutaceae), Mar.
2015, V. Guarnaccia (holotype CBS H-22598, culture ex-type CPC 26192
= CBS 141449; ITS sequence GenBank KX228262.1, LSU sequence GenBank KX228313.1, actA sequence GenBank KX228358.1, rpb2 sequence
GenBank KX228369.1, tef1 sequence GenBank KX228373.1, MycoBank
MB817020).
Colour illustrations. Glasshouse with Citrus loridana trees; conidiophores
sporulating on PNA, conidiophores and conidia. Scale bars = 10 µm.
Pedro W. Crous, Johannes Z. Groenewald & Vadimiro Guarnaccia, CBS-KNAW Fungal Biodiversity Centre,
P.O. Box 85167, 3508 AD Utrecht, The Netherlands;
e-mail: p.crous@cbs.knaw.nl, e.groenewald@cbs.knaw.nl & v.guarnaccia@cbs.knaw.nl
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
344
Persoonia – Volume 36, 2016
Vermiculariopsiella acaciae
345
Fungal Planet description sheets
Fungal Planet 411 – 4 July 2016
Vermiculariopsiella acaciae Crous, M.J. Wingf., sp. nov.
Etymology. Name refers to Acacia, the plant genus from which this fungus
was collected.
Classification — Chaetosphaeriaceae, Chaetosphaeriales,
Sordariomycetes.
Sporodochia on SNA and OA erumpent, crystalline, to 450 µm
diam, with brown, erect setae distributed throughout conidioma, thick-walled, roughened, flexuous, 180–250 × 4–5 µm,
5–7-septate, tapering to an obtuse apex. Conidiophores aggregated in stroma, subcylindrical, 1–2-septate, branched or not,
25–40 × 3–4 µm. Conidiogenous cells terminal, subcylindrical,
pale brown to hyaline, smooth to verruculose, at times curved
at the apex, 15–27 × 2.5–3 µm, apex 1.5 µm diam, collarette
flaring, 1–2 µm long. Conidia dimorphic. On OA solitary, hyaline,
guttulate, aseptate, straight to slightly curved, inequilateral with
inner plane straight, outer plane convex, apex subobtusely
rounded, base truncate with excentric hilum, 0.5–1 µm diam,
(14 –)18 – 22(– 25) × 3(– 3.5) µm. On SNA forming ellipsoid,
straight, hyaline, smooth, guttulate conidia, apex subobtusely
rounded, base truncate, 4 –7 × 2–2.5 µm.
Culture characteristics — Colonies reaching up to 40 mm
diam after 2 wk at 25 °C, with spreading, flat surface; margins
smooth, lobate, and sparse aerial mycelium. On MEA surface
pale luteous, reverse luteous. On OA surface dirty white to pale
luteous. On PDA surface and reverse dirty white.
Notes — Based on ITS DNA sequence data Vermiculariop
siella acaciae is 97 % (539/557) similar to V. dichapetali (CPC
22463; GenBank KJ869129.1; from leaves of Dichapetalum rho
desicum, Botswana) and 89 % (489/548) similar to V. eucalypti
described elsewhere in the present study. Morphologically, the
latter two species can be distinguished from V. dichapetali in
having much larger conidiogenous cells (20–40 × 2.5–3 μm), setae (100–300 × 6–10 μm, 6–12-septate), and lacking dimorphic
conidia (Crous et al. 2014a). The closest hits using a megablast
search of the LSU DNA sequence data are 99 % (816/819) similar to Vermiculariopsiella dichapetali (GenBank KJ869186.1;
incertae sedis, Microascales), 96 % (820/857) to Dictyochaeta
cylindrospora (GenBank EF063575.1; Chaetosphaeriaceae,
Chaetosphaeriales), 94 % (793/841) to Dactylaria parvispora
(GenBank EU107296.1; Orbiliaceae, Orbiliales) and 94 % (808/
858) to Cryptadelphia groenendalensis (GenBank EU528007.1;
incertae sedis).
Typus. france, La Réunion, S21°5'45.7" E55°33'3.6", on leaves of Acacia
heterophylla (Fabaceae), 7 Mar. 2015, P.W. Crous & M.J. Wingfield (holotype
CBS H-22599, culture ex-type CPC 26291 = CBS 141289; ITS sequence
GenBank KX228263.1, LSU sequence GenBank KX228314.1, MycoBank
MB817023).
Colour illustrations. Leaf spots on Acacia heterophylla; sporodochia
sporulating on OA, conidiophores, setae and dimorphic conidia. Scale bars
= 10 µm.
Pedro W. Crous, CBS-KNAW Fungal Biodiversity Centre, P.O. Box 85167, 3508 AD Utrecht, The Netherlands; e-mail: p.crous@cbs.knaw.nl
Michael J. Wingfield, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria,
Pretoria 0002, South Africa; e-mail: mike.wingfield@up.ac.za
Johannes J. Le Roux & David M. Richardson, Centre for Invasion Biology, Department of Botany & Zoology,
Stellenbosch University, Matieland 7602, South Africa; e-mail: jleroux@sun.ac.za & rich@sun.ac.za
Dominique Strasberg, Université de La Réunion, UMR PVBMT, Peuplements Végétaux et Bioagresseurs en Milieu Tropical,
15 avenue René Cassin, CS 93002, 97 744 Saint-Denis Messag. Cedex 9, La Réunion, France; e-mail: dominique.strasberg@univ-reunion.fr
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
346
Persoonia – Volume 36, 2016
Meristemomyces arctostaphylos
347
Fungal Planet description sheets
Fungal Planet 412 – 4 July 2016
Meristemomyces arctostaphylos Crous & M.J. Wingf., sp. nov.
Etymology. Name refers to Arctostaphylos, the plant genus from which
this fungus was collected.
Classification — Teratosphaeriaceae, Capnodiales, Dothi
deomycetes.
Mycelium consisting of brown, thick-walled, verruculose, branched, septate, 2.5–4 µm hyphae, frequently encased in a mucoid
sheath. Conidiophores solitary, brown, verruculose, terminal or
lateral on hyphae, multiseptate, flexuous, subcylindrical, up to
150 µm long, 3–5 µm diam, or reduced to conidiogenous loci on
hyphae. Conidiogenous cells brown, verruculose, thick-walled,
subcylindrical to irregular, 3–10 × 3–6 µm, with 1–3 terminal
flat-tipped loci, 1.5–2 µm diam. Conidia in branched chains,
brown, thick-walled, verruculose. Secondary ramoconidia
fusoid-ellipsoid to subcylindrical, 1–3-septate, 15–20 × 5–9
µm, with 1–3 flat-tipped unthickened, not darkened loci, 1.5–2
µm diam. Conidia brown, verruculose, thick-walled, 1(– 3)septate, fusoid-ellipsoid, (9–)10–12(–13) × (4–)5(–6) µm; loci
not thickened nor darkened, 1.5–2 µm diam, frequently with
minute marginal frill.
Culture characteristics — Colonies reaching up to 15 mm
diam after 2 wk at 25 °C, with spreading, erumpent, folded surface; margins smooth, lobate, and sparse aerial mycelium. On
MEA surface iron-grey, reverse olivaceous grey. On OA, PDA
and MEA surface dark mouse-grey to greenish black, reverse
greenish black.
Notes — The genus Meristemomyces is monotypic, based
on M. frigidus, isolated from rocks in the Himalayas (Egidi et al.
2014). Based on ITS sequence data, M. arctostaphylos is 91 %
(404/443) similar to the type culture of M. frigidus (CBS 136109
= CCFEE 5508; GenBank KF309961.1) and 99 % (458/460) to
Xenomeris raetica (CBS 485.61; GenBank EF114690.1). Morphologically, M. arctostaphylos is quite distinct from M. frigidus,
as the latter species produces arthoconidia by disarticulation,
while M. arctostaphylos has well-defined conidiophores giving
rise to a series of secondary ramoconidia, and septate conidia.
Xenomeris raetica was described on leaf litter of Arctostaphylos
uvaursi in Switzerland. The fungus in known only by its sexual
morph. The present collection was obtained on the same host
genus, but from the USA. Although only the asexual morph was
found, based on DNA data, it appears to be 99 % similar to a
strain identified as Xenomeris raetica (CBS 485.61), suggesting that this could be the same fungus. However, CBS 485.61
is not an ex-type strain, and M. arctostaphylos is a common
hyphomycete on leaves of Arctostaphylos. Furthermore, the
genus Xenomeris is regarded as a member of Venturiaceae,
not Teratosphaeriaceae, thus the possible synonymy of Meri
stemomyces under the older Xenomeris can only be resolved
once fresh collections of Xenomeris raetica have been obtained.
Typus. usa, Utah, near Long Valley, on leaves of Arctostaphylos patula
(Ericaceae), Oct. 2014, M.J. Wingfield (holotype CBS H-22600, culture extype CPC 25574 = CBS 141290; ITS sequence GenBank KX228264.1, LSU
sequence GenBank KX228315.1, MycoBank MB817026).
Colour illustrations. Arctostaphylos patula plants in the USA; colonies
sporulating on PDA, conidiophores and chains of conidia. Scale bars = 10 µm.
Pedro W. Crous & Johannes Z. Groenewald, CBS-KNAW Fungal Biodiversity Centre, P.O. Box 85167, 3508 AD Utrecht, The Netherlands;
e-mail: p.crous@cbs.knaw.nl & e.groenewald@cbs.knaw.nl
Michael J. Wingfield, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria,
Pretoria 0002, South Africa; e-mail: mike.wingfield@up.ac.za
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
348
Persoonia – Volume 36, 2016
Pyrenochaeta acaciae
349
Fungal Planet description sheets
Fungal Planet 413 – 4 July 2016
Pyrenochaeta acaciae Crous, Jacq. Edwards & Pascoe, sp. nov.
Etymology. Name refers to Acacia, the plant genus from which this fungus
was collected.
Classification — Cucurbitariaceae, Pleosporales, Dothideo
mycetes.
Conidiomata immersed to erumpent, solitary, globose, brown,
to 150 µm diam, with central ostiole to 35 µm diam, exuding a
caramel coloured conidial mass; wall of 2–3 layers of brown
textura angularis; ostiolar area with several cylindrical, brown,
thick-walled, septate setae, with obtuse ends to 70 µm long.
Conidiophores 0–3-septate, but mostly reduced to conidiogenous cells lining the inner cavity, hyaline, smooth, ampulliform
to subcylindrical, 4–8 × 2.5–3.5 µm, phialidic with periclinal
thickening. Conidia solitary, hyaline (pale olivaceous in mass),
smooth, aseptate, allantoid with obtuse ends, (3–)4–4.5(–5)
× (1.5–)2 µm.
Culture characteristics — Colonies reaching up to 40 mm
diam after 2 wk at 25 °C, with spreading, flat surface; margins
smooth, lobate, and sparse aerial mycelium. On MEA surface
folded, sepia with patches of isabelline and buff, reverse isabelline. On OA surface isabelline. On PDA surface and reverse
isabelline.
Notes — Based on ITS Pyrenochaeta acaciae is 98 % (409/
416) similar to P. protearum (CBS 131315; GenBank JQ044434.1)
and 98 % (425/433) to P. pinicola (CPC 23455; GenBank
KJ869152.1). Pyrenochaeta protearum can be distinguished
from P. acaciae by having setae surrounding its conidiomatal
ostiole that are longer (up to 100 µm tall), and conidia that are
wider ((3 –)4 – 5(– 6) × (2 –)2.5(– 3) μm) (Crous et al. 2011).
Pyrenochaeta pinicola is distinct by having larger conidia,
(4–)4.5–5.5(–6) × 2(–2.5) μm, and longer setae (up to 150 µm
tall) (Crous et al. 2014a).
Typus. australia, Victoria, roadside bushland opposite 125 Gurdies-St.
Helier road, The Gurdies, S38°22'49" E145°34'14", on leaves of Acacia sp.
(Fabaceae), 7 Nov. 2014, P.W. Crous, J. Edwards & I.G. Pascoe (holotype
CBS H-22601, culture ex-type CPC 25527 = CBS 141291; ITS sequence
GenBank KX228265.1, LSU sequence GenBank KX228316.1, MycoBank
MB817028).
Colour illustrations. Symptomatic leaves of Acacia sp.; conidiomata
sporulating on PDA, conidiogenous cells and conidia. Scale bars: conidioma
= 100 µm, others = 10 µm.
Pedro W. Crous & Johannes Z. Groenewald, CBS-KNAW Fungal Biodiversity Centre, P.O. Box 85167, 3508 AD Utrecht, The Netherlands;
e-mail: p.crous@cbs.knaw.nl & e.groenewald@cbs.knaw.nl
Ian G. Pascoe & Jacqueline Edwards, AgriBio, Centre for AgriBiosciences, Department of Economic Development, Jobs, Transport and Resources,
5 Ring Road, La Trobe University, Bundoora, Victoria 3083 Australia;
e-mail: pascoeig@bigpond.net.au & jacky.edwards@ecodev.vic.gov.au
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
350
Persoonia – Volume 36, 2016
Castanediella eucalypticola
& Phaeophleospora eucalypticola
351
Fungal Planet description sheets
Fungal Planet 414 & 415 – 4 July 2016
Castanediella eucalypticola Crous & M.J. Wingf., sp. nov.
Etymology. Name refers to Eucalyptus, the plant genus from which this
fungus was collected.
Classification — Incertae sedis, Xylariales, Sordariomy
cetes.
Mycelium consisting of pale brown, branched, septate hyphae,
2–5 µm diam, frequently in hyphal strands, and forming hyphal
coils. Conidiophores erect, solitary, unbranched, 0–2-septate,
subcylindrical, medium brown, smooth, 5–30 × 3–5 µm. Conidi
ogenous cells terminal and intercalary, subcylindrical to ampulliform or lanceolate, pale brown, smooth, polyblastic, terminating
in a swollen apex, 1.5–3 µm diam, with several scars, 5–20 ×
3–3.5 µm. Conidia solitary, hyaline, smooth, falcate, straight
to curved, widest in the middle, apex subobtusely rounded,
base truncate, 0.5 µm diam, (15 –)20–26(–30) × (2.5–)3 µm.
Culture characteristics — Colonies reaching up to 30 mm
diam after 2 wk at 25 °C, with spreading, erumpent surface;
margins smooth, lobate, and sparse aerial mycelium. On MEA
surface sepia, reverse isabelline. On OA surface cinnamon.
On PDA surface honey, reverse isabelline.
Typus. france, La Réunion, on leaves of Eucalyptus robusta (Myrtaceae),
9 Mar. 2015, P.W. Crous & M.J. Wingfield (holotype CBS H-22604, culture
ex-type CPC 26539 = CBS 141317; ITS sequence GenBank KX228266.1,
LSU sequence GenBank KX228317.1, tub2 sequence GenBank KX228382.1,
MycoBank MB817029).
Notes — On ITS Castanediella eucalypticola is 98 % (547/
556) similar to C. eucalypti (CPC 24746; GenBank KR476723.1),
and 95 % (523/552) to C. couratarii (CBS 579.71; GenBank
KP859050.1) (Crous et al. 2015a). Castanediella eucalypticola
can be distinguished from C. eucalypti, by the fact that the latter
has smaller conidia, (15–)18–21(–23) × 2–3 µm, and branched
conidiophores (Crous et al. 2015a).
Phaeophleospora eucalypticola Crous & M.J. Wingf., sp. nov.
Etymology. Name refers to Eucalyptus, the plant genus from which this
fungus was collected.
Classification — Mycosphaerellaceae, Capnodiales, Dothi
deomycetes.
Conidiomata (on pine needle agar; PNA) pycnidial, erumpent,
solitary, brown, globose, to 300 µm diam, with central ostiole,
to 20 µm diam; wall of 2–3 layers of brown textura angularis.
Conidiophores lining the inner cavity, hyaline, smooth, reduced
to conidiogenous cells, or with a supporting cell, branched at
base or not, ampulliform to subcylindrical, 5–10 × 2.5–3.5 µm,
proliferating inconspicuously percurrently at apex, 1 µm diam.
Conidia hyaline, smooth, solitary, aseptate, guttulate, ellipsoid
to obovoid, base truncate, 0.5 µm diam, (3.5–)4.5–6(–7) ×
(1.5–)2(–2.5) µm.
Culture characteristics — Colonies reaching up to 15 mm
diam after 2 wk at 25 °C, with spreading, erumpent surface;
margins smooth, lobate, and moderate aerial mycelium. On
MEA, PDA and OA, surface and reverse mouse-grey.
Typus. france, La Réunion, Le Tampon, on leaves of Eucalyptus robusta
(Myrtaceae), 9 Mar. 2015, P.W. Crous & M.J. Wingfield (holotype CBS
H-22605, culture ex-type CPC 26523 = CBS 141294; ITS sequence GenBank
KX228267.1, LSU sequence GenBank KX228318.1, tef1 sequence GenBank
KX228374.1, MycoBank MB817030).
Notes — Phaeophleospora (based on P. eugeniae; Crous
et al. 1997) is a genus in the Mycosphaerellaceae, which is
distinct from Teratosphaeria and its various asexual morphs
(Quaedvlieg et al. 2014). Based on LSU DNA sequence data
Phaeophleospora eucalypticola is identical (100 %) (818/818)
to P. hymenocallidicola (CPC 25014; GenBank KR476772.1)
and 99 % (846/849) similar to P. eugeniae (CPC 15159; GenBank FJ493207.1) (Crous et al. 2015a). Based on ITS sequence
data it is 94 % (453/482) similar to P. pteridivora (COAD 1182;
GenBank KT037547.1), though the latter species has conidia
that are subcylindrical, curved to sinuous, 70–107 × 2–3 μm,
6–9-septate (Guatimosim et al. 2016). The ITS sequence of
P. eucalypticola is similar to numerous sequences in GenBank
labelled as ‘Mycosphaerella sp. AA-2012’ which emerged from
an unpublished study on endophytic fungi from leaves of an
Eucalyptus grandis × E. camaldulensis clone in South Africa.
Colour illustrations. Eucalyptus trees growing on La Réunion; Phaeophleo
spora eucalypticola (left column): colonies sporulating on OA, conidiogenous
cells and conidia; Castanediella eucalypticola (right column): colony sporulating on OA, conidiophores and conidia. Scale bars = 10 µm.
Pedro W. Crous, CBS-KNAW Fungal Biodiversity Centre, P.O. Box 85167, 3508 AD Utrecht, The Netherlands; e-mail: p.crous@cbs.knaw.nl
Michael J. Wingfield, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria,
Pretoria 0002, South Africa; e-mail: mike.wingfield@up.ac.za
Johannes J. Le Roux & David M. Richardson, Centre for Invasion Biology, Department of Botany & Zoology,
Stellenbosch University, Matieland 7602, South Africa; e-mail: jleroux@sun.ac.za & rich@sun.ac.za
Dominique Strasberg, Université de La Réunion, UMR PVBMT, Peuplements Végétaux et Bioagresseurs en Milieu Tropical,
15 avenue René Cassin, CS 93002, 97 744 Saint-Denis Messag. Cedex 9, La Réunion, France;
e-mail: dominique.strasberg@univ-reunion.fr
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
352
Persoonia – Volume 36, 2016
Sclerostagonospora ericae
353
Fungal Planet description sheets
Fungal Planet 416 – 4 July 2016
Sclerostagonospora ericae Crous & M.J. Wingf., sp. nov.
Etymology. Name refers to Erica, the plant genus from which this fungus
was collected.
Classification — Phaeosphaeriaceae, Pleosporales, Dothi
deomycetes.
Conidiomata (on pine needle agar; PNA) pycnidial, solitary or
in small clusters, immersed or semi-erumpent, to 200 µm diam,
globose, pale brown, with 1–3 dark brown, semi-papillate ostioles, to 40 µm diam; wall of 3–4 layers of pale brown textura
angularis. Conidiophores reduced to conidiogenous cells lining
the inner cavity, hyaline, smooth, ampulliform to doliiform, 4–5
× 3–5 µm, proliferating inconspicuously percurrently at apex.
Conidia solitary, pale brown, smooth, subcylindrical, guttulate,
1(–3)-septate, constricted at median septum, (7–)8–10(–11)
× (2.5–)3 µm.
Culture characteristics — Colonies reaching up to 60 mm
diam after 2 wk at 25 °C, with spreading, flat surface; margins
smooth, lobate, and moderate aerial mycelium. On MEA surface
dirty white to buff, reverse sepia. On OA surface buff. On PDA
surface buff, reverse cinnamon.
Notes — Based on LSU sequences, Sclerostagonospora
ericae is identical (813/813) to S. opuntiae (GenBank DQ286772.1; Huhndorf 1992). However, the genus Sclerostagono
spora is based on S. heraclei, and the latter is presently not
known from DNA or culture, hence the concept of Sclero
stagonospora remains unsettled. On ITS S. ericae is 98 %
(550/560) similar to S. opuntiae (GenBank DQ286768.1) and
94 % (541/574) similar to Stagonospora foliicola (GenBank
KF251256.1).
Typus. south africa, Western Cape Province, Franschhoek, on leaves
of Erica sp. (Ericaceae), Nov. 2014, M.J. Wingfield (holotype CBS H-22606,
culture ex-type CPC 25927 = CBS 141318; ITS sequence GenBank
KX228268.1, LSU sequence GenBank KX228319.1, tef1 sequence GenBank
KX228375.1, tub2 sequence GenBank KX228383.1, MycoBank MB817031).
Colour illustrations. Erica sp. in Franschhoek; conidiomata sporulating on
OA, conidiogenous cells, ostiolar region and conidia. Scale bars: conidiomata
and ostioles = 200 µm, all others = 10 µm.
Pedro W. Crous & Johannes Z. Groenewald, CBS-KNAW Fungal Biodiversity Centre, P.O. Box 85167, 3508 AD Utrecht, The Netherlands;
e-mail: p.crous@cbs.knaw.nl & e.groenewald@cbs.knaw.nl
Michael J. Wingfield, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria,
Pretoria 0002, South Africa; e-mail: mike.wingfield@up.ac.za
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
354
Persoonia – Volume 36, 2016
Dothiorella acacicola
355
Fungal Planet description sheets
Fungal Planet 417 – 4 July 2016
Dothiorella acacicola Crous & M.J. Wingf., sp. nov.
Etymology. Name refers to Acacia, the plant genus from which this fungus
was collected.
Classification — Botryosphaeriaceae, Botryosphaeriales,
Dothideomycetes.
Conidiomata (on pine needle agar; PNA) pycnidial, solitary,
black, erumpent, globose, to 400 µm diam, with central ostiole;
wall of 2–3 layers of brown textura angularis. Conidiophores
reduced to conidiogenous cells, or with a supporting cell, lining
the inner cavity, hyaline, smooth, subcylindrical to doliiform,
straight to geniculous-sinuous, 7–20 × 5–11 µm; proliferating
percurrently at apex. Conidia solitary, initially hyaline, becoming pigmented while attached to conidiogenous cells, goldenbrown to brown, granular to guttulate, surface roughened (at
times with hyaline outer sheath), obovoid, medianly 1-septate,
slightly constricted at septum, apex obtuse, base truncate, 3–5
µm diam, (22–)24–27(–32) × (9–)10(–11) µm.
Culture characteristics — Colonies covering dish after 2 wk
at 25 °C, with spreading, flat surface; margins smooth, lobate,
and moderate aerial mycelium. On MEA, PDA and OA surface
and reverse dark mouse-grey.
Notes — Based on LSU sequence data Dothiorella acacicola
is 99 % similar to several Dothiorella spp. (e.g. 791/801 with
D. ulmicola GenBank KR611900.1). Based on ITS sequence
data Dothiorella acacicola is 97 % (523/538) similar to D. longi
collis (CBS 122068; GenBank KF766162.1). The two species
can be distinguished from each other by the fact that conidia of
D. longicollis are smaller, (17–)19–22(–23) × (7–)8.5–9.5(–10)
µm (Phillips et al. 2013).
Typus. france, La Réunion, S21°12'47.6" E55°36'48.7", on leaves of Aca
cia mearnsii (Fabaceae), 8 Mar. 2015, P.W. Crous & M.J. Wingfield (holotype
CBS H-22607, culture ex-type CPC 26349 = CBS 141295; ITS sequence
GenBank KX228269.1, LSU sequence GenBank KX228320.1, tef1 sequence
GenBank KX228376.1, MycoBank MB817032).
Colour illustrations. Acacia mearnsii trees growing in La Réunion; conidiomata sporulating on PNA, conidiogenous cells and conidia. Scale bars =
10 µm.
Pedro W. Crous, CBS-KNAW Fungal Biodiversity Centre, P.O. Box 85167, 3508 AD Utrecht, The Netherlands; e-mail: p.crous@cbs.knaw.nl
Michael J. Wingfield, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria,
Pretoria 0002, South Africa; e-mail: mike.wingfield@up.ac.za
Johannes J. Le Roux & David M. Richardson, Centre for Invasion Biology, Department of Botany & Zoology,
Stellenbosch University, Matieland 7602, South Africa; e-mail: jleroux@sun.ac.za & rich@sun.ac.za
Dominique Strasberg, Université de La Réunion, UMR PVBMT, Peuplements Végétaux et Bioagresseurs en Milieu Tropical,
15 avenue René Cassin, CS 93002, 97 744 Saint-Denis Messag. Cedex 9, La Réunion, France;
e-mail: dominique.strasberg@univ-reunion.fr
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
356
Persoonia – Volume 36, 2016
Chalara clidemiae
357
Fungal Planet description sheets
Fungal Planet 418 – 4 July 2016
Chalara clidemiae Crous & M.J. Wingf., sp. nov.
Etymology. Name refers to Clidemia, the plant genus from which this
fungus was collected.
Classification — Incertae sedis, Helotiales, Leotiomycetes.
Mycelium consisting of hyaline, smooth, branched, septate,
1.5–2.5 µm diam hyphae. Conidiophores arranged in terminal
clusters on hyphae, 25–30 × 3–4 µm, 1–5-septate, subcylindrical, smooth, hyaline in bottom half, but upper two cells medium
brown. Conidiogenous cells terminal, swollen in bottom third,
venter cylindrical, brown, smooth, phialidic, 15 – 25 × 3.5 – 4
µm. Conidia hyaline, smooth, guttulate, subcylindrical, apex obtuse, base truncate, (3–)4(–5) × (2–)2.5 µm, forming long, curvy
chains or slimy masses on older phialides.
Culture characteristics — Colonies reaching up to 30 mm
diam after 2 wk at 25 °C, with spreading, flat surface; margins
smooth, lobate, and sparse to moderate aerial mycelium. On
MEA surface dirty white, reverse buff. On OA surface ochreous.
On PDA surface luteous, reverse ochreous.
Notes — The genus Chalara is paraphyletic, with species occupying different positions within Helotiales (Cai et al.
2009). Based on ITS sequence data Chalara clidemiae is 91 %
(492/538) similar to Chalara pseudoaffinis (CCF 3979; GenBank FR667224.1). On LSU it is 98 % (841/855) similar to Cha
lara africana (OC0018; GenBank FJ176249.1) and 96 % (821/
854) to Chalara parvispora (CBS 385.94; GenBank FJ176253.1).
Typus. france, La Réunion, on twigs of Clidemia hirta (Melastomataceae),
6 Mar. 2015, P.W. Crous & M.J. Wingfield (holotype CBS H-22608, culture
ex-type CPC 26423 = CBS 141319; ITS sequence GenBank KX228270.1,
LSU sequence GenBank KX228321.1, MycoBank MB817033).
Colour illustrations. Clidemia hirta on La Réunion; conidiophores sporulating on PNA, phialides and conidia. Scale bars = 10 µm.
Pedro W. Crous, CBS-KNAW Fungal Biodiversity Centre, P.O. Box 85167, 3508 AD Utrecht, The Netherlands; e-mail: p.crous@cbs.knaw.nl
Michael J. Wingfield, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria,
Pretoria 0002, South Africa; e-mail: mike.wingfield@up.ac.za
Johannes J. Le Roux & David M. Richardson, Centre for Invasion Biology, Department of Botany & Zoology,
Stellenbosch University, Matieland 7602, South Africa; e-mail: jleroux@sun.ac.za & rich@sun.ac.za
Dominique Strasberg, Université de La Réunion, UMR PVBMT, Peuplements Végétaux et Bioagresseurs en Milieu Tropical,
15 avenue René Cassin, CS 93002, 97 744 Saint-Denis Messag. Cedex 9, La Réunion, France;
e-mail: dominique.strasberg@univ-reunion.fr
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
358
Persoonia – Volume 36, 2016
Mulderomyces natalis
359
Fungal Planet description sheets
Fungal Planet 419 – 4 July 2016
Mulderomyces Crous, Jacq. Edwards & P.W.J. Taylor, gen. nov.
Etymology. Named after Prof. dr. Theo W. Mulder, the scientific director
of the institutes of the Royal Dutch Academy of Arts and Sciences (KNAW),
on the occasion of his farewell symposium, 20 June 2016.
Classification — Incertae sedis, Ostropales, Lecanoromy
cetes.
Conidiomata pycnidial, solitary, pale brown, erumpent, globose,
with central ostiole; wall of 6–8 layers of subhyaline to pale
brown textura angularis. Conidiophores lining the inner cavity,
hyaline, smooth, subcylindrical, septate, branched. Conidiog
enous cells hyaline, smooth, subcylindrical, terminal and lateral; proliferating sympodially, scars inconspicuous. Conidia
cylindrical, hyaline, smooth, guttulate, straight with subobtuse
ends, 2 – 6-septate, prominently constricted at septa (cells
linked by a narrow isthmus), with mature conidia breaking into
phragmospores.
Type species. Mulderomyces natalis Crous, Jacq. Edwards & P.W.J. Taylor.
MycoBank MB817034.
Mulderomyces natalis Crous, Jacq. Edwards & P.W.J. Taylor, sp. nov.
Etymology. Natalis (Latin genitive noun), refers to the birth date of the
first author, on which day this fungus was collected.
Conidiomata (on pine needle agar; PNA) pycnidial, solitary, pale
brown, erumpent, globose, to 200 µm diam, with central ostiole;
wall of 6–8 layers of subhyaline to pale brown textura angularis.
Conidiophores lining the inner cavity, hyaline, smooth, subcylindrical, 1–4-septate, branched, 10–30 × 3–5 µm. Conidiog
enous cells hyaline, smooth, subcylindrical, terminal and lateral,
8–15 × 3–5 µm; proliferating sympodially, scars inconspicuous.
Conidia cylindrical, hyaline, smooth, guttulate, straight with subobtuse ends, 2 – 6-septate, prominently constricted at septa
(cells linked by a narrow isthmus), with mature conidia breaking
into phragmospores, (22 –)50–75(–90) × (2–)3 µm.
Culture characteristics — Colonies reaching up to 20 mm
diam after 2 wk at 25 °C, with spreading, flat surface; margins
smooth, lobate, and sparse aerial mycelium. On MEA surface
cinnamon, reverse brick. On OA surface rosy buff. On PDA
surface rosy buff, reverse cinnamon.
Notes — The LSU sequence of Mulderomyces natalis is
91 % similar to species of Xylographa (lichenised ascomycetes;
e.g. 738/810 to X. opegraphella GenBank KJ462366.1) and
92 % (746/812) similar to Furcaspora eucalypti (GenBank
EF110613; Crous et al. 2007c). Phylogenetically, it appears
quite distinct from all taxa presently available in GenBank, both
on LSU and ITS. Morphologically, Mulderomyces resembles
species of Phacidiella, except that the nature of its conidia
is different with cells not linked by a narrow isthmus, and its
conidiomata become cupulate with age (Sutton 1980).
Typus. australia, Victoria, Melbourne, Moonee Ponds Creek, on leaves
of Eucalyptus sp. (Myrtaceae), 2 Nov. 2014, P.W. Crous, J. Edwards & P.W.J.
Taylor (holotype CBS H-22609, culture ex-type CPC 25519 = CBS 141296;
ITS sequence GenBank KX228271.1, LSU sequence GenBank KX228322.1,
MycoBank MB817035).
Colour illustrations. Eucalyptus tree growing along river at Moonee Ponds
Creek; conidiomata sporulating on OA, conidiophores and conidia. Scale
bars = 10 µm.
Pedro W. Crous & Johannes Z. Groenewald, CBS-KNAW Fungal Biodiversity Centre, P.O. Box 85167, 3508 AD Utrecht, The Netherlands;
e-mail: p.crous@cbs.knaw.nl & e.groenewald@cbs.knaw.nl
Jacqueline Edwards, AgriBio, Centre for AgriBiosciences, Department of Economic Development, Jobs, Transport and Resources,
5 Ring Road, La Trobe University, Bundoora, Victoria 3083, Australia; e-mail: jacky.edwards@ecodev.vic.gov.au
Paul W.J. Taylor, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Australia; e-mail: paulwjt@unimelb.edu.au
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
360
Persoonia – Volume 36, 2016
Wojnowiciella cissampeli
& Diaporthe cissampeli
361
Fungal Planet description sheets
Fungal Planet 420 & 421 – 4 July 2016
Wojnowiciella cissampeli Crous & Roets, sp. nov.
Etymology. Name refers to Cissampelos, the plant genus from which this
fungus was collected.
Classification — Phaeosphaeriaceae, Pleosporales, Dothi
deomycetes.
Conidiomata (on pine needle agar; PNA) pycnidial, solitary,
black, erumpent, or immersed in agar, globose, to 300 µm
diam, non-papillate, with a central ostiole; pycnidial wall of 3–6
layers of brown textura angularis. Conidiophores reduced to
conidiogenous cells. Conidiogenous cells lining the inner cavity,
hyaline, smooth, ampulliform to doliiform, phialidic, 3–6 × 4–5
µm. Conidia subcylindrical, straight to curved, apex subobtuse,
base truncate, widest in middle, (0–)3–7-septate, rarely with
1–2 oblique septa, thick-walled, verruculose, guttulate, goldenbrown, (20 –)22–25(–27) × (4.5–)5(–6) µm.
Culture characteristics — Colonies reaching up to 20 mm
diam after 2 wk at 25 °C, with spreading, flat surface; margins
smooth, lobate, and moderate aerial mycelium. On MEA surface
mouse-grey to greyish sepia, reverse greyish sepia. On OA
surface pale mouse-grey in centre, sienna in outer region. On
PDA surface pale mouse-grey, reverse greyish sepia.
Typus. south africa, Western Cape Province, Robben Island, on leaves
and twigs of Cissampelos capensis (Menispermaceae), May 2015, P.W.
Crous & F. Roets (holotype CBS H-22610, culture ex-type CPC 27455 =
CBS 141297; ITS sequence GenBank KX228272.1, LSU sequence GenBank
KX228323.1, MycoBank MB817036).
Notes — On ITS Wojnowiciella cissampeli is 98 % (565/577)
similar to W. eucalypti (CPC 25024; GenBank KR476741.1), and
99 % (546/552) to Wojnowicia lonicerae (MFLUCC 13-0737;
GenBank KP744471.1). Morphologically, conidia of W. cis
sampeli are smaller than those of W. eucalypti, (10 –)28 –
30(–33) × (4–)6–7 µm (Crous et al. 2015a), and Wojnowicia
lonicerae (38–(42)–49 × 5–(5.5)–6 μm) (Liu et al. 2015).
Diaporthe cissampeli Crous & Roets, sp. nov.
Etymology. Name refers to Cissampelos, the plant genus from which this
fungus was collected.
Classification — Diaporthaceae, Diaporthales, Sordariomy
cetes.
Conidiomata (on pine needle agar; PNA) pycnidial, solitary,
black, erumpent, globose, to 200 µm diam, exuding creamy
droplets from central ostioles; walls consisting of 3–6 layers
of medium brown textura angularis. Conidiophores hyaline,
smooth, 1–2-septate, branched, densely aggregated, subcylindrical, straight to sinuous, 12–20 × 3–5 µm. Conidiogenous
cells 7–10 × 2–3 µm, phialidic, cylindrical, terminal and lateral
with slight taper towards apex, 1–1.5 µm diam, with visible
periclinal thickening; collarette not observed. Paraphyses not
observed. Alpha conidia aseptate, hyaline, smooth, guttulate,
subcylindrical, tapering towards both ends, apex subobtuse,
base subtruncate, (7.5–)9–11(–12) × (2–)2.5(–3) µm. Gamma
conidia not observed. Beta conidia not observed.
Culture characteristics — Colonies covering dish after 2 wk
at 25 °C, with smooth, even margins, and moderate aerial
mycelium. On MEA surface dirty white with patches of pale
mouse-grey, reverse luteous. On OA and PDA surface and
reverse dirty white.
Typus. south africa, Western Cape Province, Robben Island, on leaves
and twigs of Cissampelos capensis (Menispermaceae), May 2015, P.W.
Crous & F. Roets (holotype CBS H-22628, culture ex-type CPC 27302 =
CBS 141331; ITS sequence GenBank KX228273.1, LSU sequence GenBank
KX228324.1, his3 sequence GenBank KX228366.1, tub2 sequence GenBank
KX228384.1, MycoBank MB817059).
Notes — Based on ITS Diaporthe cissampeli is 98 % (561/
575) similar to D. neotheicola (ICMP 10076; GenBank KC145914.1; Gomes et al. 2013). No high similarity (> 99 %) hits
were obtained when the protein coding sequences were blasted
against NCBIs GenBank nucleotide database. No other species
of Diaporthe are known from Cissampelos (Crous et al. 2000)
and hence D. cissampeli is herewith introduced as new.
Colour illustrations. Cissampelos capensis growing on Robben Island;
Wojnowiciella cissampeli (left column): conidiomata sporulating on OA (scale
bar = 300 µm), conidiogenous cells and conidia; Diaporthe cissampeli (right
column): conidiomata sporulating on PNA (scale bar = 200 µm), conidiogenous cells and conidia. Scale bars = 10 µm.
Pedro W. Crous & Johannes Z. Groenewald, CBS-KNAW Fungal Biodiversity Centre, P.O. Box 85167, 3508 AD Utrecht, The Netherlands;
e-mail: p.crous@cbs.knaw.nl & e.groenewald@cbs.knaw.nl
Francois Roets, Department of Conservation Ecology and Entomology, Stellenbosch University, South Africa; e-mail: fr@sun.ac.za
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
362
Persoonia – Volume 36, 2016
Prosopidicola albizziae
363
Fungal Planet description sheets
Fungal Planet 422 – 4 July 2016
Prosopidicola albizziae Crous & M.J. Wingf., sp. nov.
Etymology. Name refers to Albizzia, the host genus from which this fungus
was collected.
Classification — Incertae sedis, Diaporthales, Sordariomy
cetes.
Conidiomata (on pine needle agar; PNA) pycnidial, separate
or aggregated in an eustromatic stroma with one to several
ostioles, mouse-grey, erumpent, to 300 µm diam; wall up to 10
layers of grey-brown textura angularis. Conidiophores reduced
to conidiogenous cells. Conidiogenous cells tightly aggregated,
hyaline, smooth, ampulliform, 5–12 × 3–4 µm, mono- to polyphialidic, with 1–2 apical loci with visible periclinal thickening, at
times with percurrent proliferation. Conidia solitary, subhyaline
to grey-brown, smooth, guttulate, straight to variously curved, ellipsoid to fusoid-ellipsoid, apex obtuse, base truncate to bluntly
rounded, 1–1.5 µm diam, (5–)7–9(–11) × (2.5–)3–3.5(–4) µm.
Culture characteristics — Colonies reaching up to 20–30
mm diam after 2 wk at 25 °C, with spreading, flat surface; margins uneven, feathery, and sparse to moderate aerial mycelium.
On MEA, PDA and OA surface and reverse pale mouse-grey.
Notes — Phylogenetically, CPC 27478 is identical to CPC
27484. Based on LSU sequence data, Prosopidicola albizziae
is 99 % (807/815) similar to P. mexicana, which was regarded
as a potential biocontrol agent of Prosopis glandulosa, causing a pod disease of this host in Mexico and the USA (Texas)
(Lennox et al. 2004). However, on ITS the two species are
only 84 % (512/607) similar. Morphologically, P. albizziae has
smaller conidia than those of P. mexicana, (8–)10–13(–20) ×
(3.5–)4.5–5.5(–6) µm. Prosopidicola was noted to have conidiogenous cells that vary from being phialidic with periclinal
thickening, or with prominent percurrent proliferation, becoming
darkened at the apex (Lennox et al. 2004).
Typus. Malaysia, Sabah, Tawau, on leaves of Albizzia falcataria (Faba
ceae), May 2015, M.J. Wingfield (holotype CBS H-22611, culture ex-type CPC
27478 = CBS 141298; ITS sequence GenBank KX228274.1, LSU sequence
GenBank KX228325.1, MycoBank MB817037); ibid., associated with
stem cankers on Albizzia falcataria, CPC 27484 (ITS sequence GenBank
KX228275.1, LSU sequence GenBank KX228326.1, tub2 sequence GenBank KX228385.1).
Colour illustrations. Albizzia falcataria trees; conidiomata sporulating on
PNA, conidiogenous cells and conidia. Scale bars = 10 µm.
Pedro W. Crous & Johannes Z. Groenewald, CBS-KNAW Fungal Biodiversity Centre, P.O. Box 85167, 3508 AD Utrecht, The Netherlands;
e-mail: p.crous@cbs.knaw.nl & e.groenewald@cbs.knaw.nl
Michael J. Wingfield, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria,
Pretoria 0002, South Africa; e-mail: mike.wingfield@up.ac.za
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
364
Persoonia – Volume 36, 2016
Phaeotheca salicorniae
365
Fungal Planet description sheets
Fungal Planet 423 – 4 July 2016
Phaeotheca salicorniae Crous & Roets, sp. nov.
Etymology. Name refers to Salicornia, the plant genus from which this
fungus was collected.
Classification — Incertae sedis, Capnodiales, Dothideomy
cetes.
Mycelium consisting of hyaline, smooth, septate, branched,
3–5 µm diam hyphae, that swell in areas up to 20 µm diam,
terminal or intercalary, and develop numerous endoconidia,
brown, verruculose, globose to obovoid, muriformly septate,
5–8 µm diam, bursting open to release several endoconidia that
are red-brown, verruculose, aseptate, ellipsoid to subglobose
or irregularly, 3–6 × 3–5 µm.
Culture characteristics — Colonies reaching up to 10 mm
diam after 2 wk at 25 °C, with spreading, flat surface; margins
smooth, lobate, and lacking aerial mycelium. On MEA, PDA
and OA surface and reverse black.
Notes — Based on the LSU sequence data, Phaeotheca
salicorniae is 99 % (828/834) similar to Phaeotheca triangularis
(CBS 471.90; GenBank EU019279.1). On ITS the two species
are 99 % (828/834) similar. The genus Phaeotheca presently
includes three species, with P. triangularis (isolated from an
air-conditioning system in Belgium) being characterised by ellipsoid to subglobose to triangular endoconidia. In P. salicorniae
the endoconidia differ in shape and are slightly larger, 5.5–7
× 4.5–5.5 µm (De Hoog et al. 1997), and hyphal swellings up
are to 70 µm diam. The genus Phaeotheca is polyphyletic, and
its taxonomy will be revised in a separate study (J. Bezerra et
al. in prep).
Typus. south africa, Western Cape Province, Robben Island, on leaves
and twigs of Salicornia meyeriana (Amaranthaceae), May 2015, P.W. Crous &
F. Roets (holotype CBS H-22612, culture ex-type CPC 27406 = CBS 141299;
ITS sequence GenBank KX228276.1, LSU sequence GenBank KX228327.1,
MycoBank MB817038).
Colour illustrations. Salicornia sp.; hyphae with endoconidia on PNA.
Scale bars = 10 µm.
Pedro W. Crous & Johannes Z. Groenewald, CBS-KNAW Fungal Biodiversity Centre, P.O. Box 85167, 3508 AD Utrecht, The Netherlands;
e-mail: p.crous@cbs.knaw.nl & e.groenewald@cbs.knaw.nl
Francois Roets, Department of Conservation Ecology and Entomology, Stellenbosch University, South Africa; e-mail: fr@sun.ac.za
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
366
Persoonia – Volume 36, 2016
Paracylindrocarpon aloicola
367
Fungal Planet description sheets
Fungal Planet 424 – 4 July 2016
Paracylindrocarpon Crous, Roets & L. Lombard, gen. nov.
Etymology. Name reflects a morphological similarity to the genus Cylin
drocarpon.
Classification — Bionectriaceae, Hypocreales, Sordariomy
cetes.
Mycelium consisting of hyaline, smooth, branched, septate
hyphae, forming hyphal coils. Conidiophores solitary, hyaline,
smooth, erect, straight to geniculate-sinuous, arising from
superficial hyphae, unbranched or branched, septate. Conidi
ogenous cells hyaline, smooth, subcylindrical with slight apical
taper, straight to slightly irregularly curved, terminal or lateral
on conidiophores, apex with minute periclinal thickening. Co
nidia hyaline, smooth, granular, cylindrical, apex obtuse, base
truncate, (0–)3-septate.
Type species. Paracylindrocarpon aloicola Crous, Roets & L. Lombard.
MycoBank MB817039.
Paracylindrocarpon aloicola Crous, Roets & L. Lombard, sp. nov.
Etymology. Name refers to Aloe, the plant genus from which this fungus
was collected.
Mycelium consisting of hyaline, smooth, branched, septate, 3–4
µm diam hyphae, forming hyphal coils. Conidiophores solitary,
hyaline, smooth, erect, straight to geniculate-sinuous, arising
from superficial hyphae, unbranched or branched, 1–4-septate,
20–50 × 3–4 µm. Conidiogenous cells hyaline, smooth, subcylindrical with slight apical taper, straight to slightly irregularly
curved, terminal or lateral on conidiophores, 20–30 × 2.5–3
µm, apex with minute periclinal thickening, 1.5–2 µm diam.
Conidia hyaline, smooth, granular, cylindrical, apex obtuse,
base truncate, 1 µm diam, (0–)3-septate, (17–)20–24(–27)
× (2.5–)3 µm.
Culture characteristics — Colonies reaching up to 30 mm
diam on PDA and OA, 15 mm diam on MEA after 2 wk at 25 °C,
with spreading, flat surface; margins smooth, lobate, and sparse
to moderate aerial mycelium. On MEA surface pale luteous with
patches of saffron, and diffuse scarlet pigment in agar, reverse
amber. On PDA and OA surface and reverse pale luteous.
Notes — Based on LSU Paracylindrocarpon aloicola has
99 % (791/ 794) similarity to Hydropisphaera erubescens (ATCC
36093; GenBank AF193230.1). Likewise, ITS is 99 % (559/567)
similar to Hydropisphaera erubescens (GenBank FJ969800.1)
and 94 % (536/572) to Fusariella sinensis (OUCMBI110131;
GenBank KP269041.1). The genus Hydropisphaera is based on
H. peziza, which has an Acremonium asexual morph (Samuels
1976). Hydropisphaera erubescens has a cylindrocarpon-like
asexual morph with 1–3-septate conidia (Samuels 1978), showing some resemblance to the present collection, suggesting
that some members of Hydropisphaera would be better accommodated in Paracylindrocarpon once the genus has been
revised. The genus Cylindrocarpon (Nectriaceae) has recently
been revised, and shown to be polyphyletic (Halleen et al. 2004,
Chaverri et al. 2011, Lombard et al. 2014), with Paracylindro
carpon (Bionectriaceae) representing yet an additional genus
in this complex.
Typus. south africa, Western Cape Province, Robben Island, on leaves
and twigs of Aloe sp. (Xanthorrhoeaceae), May 2015, P.W. Crous & F. Roets
(holotype CBS H-22613, culture ex-type CPC 27362 = CBS 141300; ITS sequence GenBank KX228277.1, LSU sequence GenBank KX228328.1, tub2
sequence GenBank KX228386.1, MycoBank MB817040).
Colour illustrations. Symptomatic leaves of Aloe sp. growing on top of
old gun turret on Robben Island; colony sporulating on PNA, conidiophores
and conidia. Scale bars = 10 µm.
Pedro W. Crous, Johannes Z. Groenewald & Lorenzo Lombard, CBS-KNAW Fungal Biodiversity Centre,
P.O. Box 85167, 3508 AD Utrecht, The Netherlands;
e-mail: p.crous@cbs.knaw.nl, e.groenewald@cbs.knaw.nl & l.lombard@cbs.knaw.nl
Francois Roets, Department of Conservation Ecology and Entomology, Stellenbosch University, South Africa; e-mail: fr@sun.ac.za
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
368
Persoonia – Volume 36, 2016
Fusicladium eucalypticola
369
Fungal Planet description sheets
Fungal Planet 425 – 4 July 2016
Fusicladium eucalypticola Crous & M.J. Wingf., sp. nov.
Etymology. Name refers to Eucalyptus, the plant genus from which this
fungus was collected.
Classification — Sympoventuriaceae, Venturiales, Dothideo
mycetes.
Mycelium consisting of pale brown, smooth, branched, septate,
1.5–3 µm diam hyphae. Conidiophores reduced to conidiogenous cells, or with basal supporting cell. Conidiogenous cells
erect, brown, smooth, subcylindrical, straight, 5–20 × 2.5–3
µm, with a single terminal locus, thickened and darkened, 1–2
µm diam. Primary ramoconidia brown, smooth, subcylindrical,
0–2-septate, 15–25 × 3 µm, with 1–2 apical loci. Secondary
ramoconidia brown, smooth, subcylindrical to fusoid-ellipsoid,
15 – 20 × 3 – 5 µm, 1– 3-septate; loci thickened, darkened,
0.5–1 µm diam. Conidia occurring in branched chains of up
to 15 conidia, fusoid-ellipsoid, pale brown, smooth, guttulate,
aseptate, (5 –)7–10(–12) × (2.5 –)3(– 4) µm; hila thickened,
darkened, 0.5 µm diam.
Culture characteristics — Colonies reaching up to 15 mm
diam after 2 wk at 25 °C, with spreading, flat surface; margins
smooth, lobate, and moderate aerial mycelium. On MEA, PDA
and OA surface and reverse sienna.
Notes — The LSU sequence of Fusicladium eucalypticola
is 99 % (868/871) similar to F. eucalypti (CBS 128216; GenBank HQ599601.1) and 94 % (824/872) to F. africanum (CPC
12829; GenBank EU035424.1). Based on ITS DNA sequence
data F. eucalypticola is 99 % (532/539) similar to F. eucalypti
(CBS 128216; GenBank HQ599600.1) and 86 % (319/371) to
F. convolvularum (CPC 3884; GenBank AY251082.1). Fusicla
dium eucalypti (on Eucalyptus sp., Queensland, Australia) has
smaller secondary ramoconidia, being 0–1-septate, (10–)12–
13(–15) × (2–)2.5–3 µm, and narrower conidia, (7–)8–9(–10)
× (2–)2.5(–3) µm (Crous et al. 2010a).
Typus. france, La Réunion, on leaves of Eucalyptus robusta (Myrtaceae),
8 Mar. 2015, P.W. Crous & M.J. Wingfield (holotype CBS H-22614, culture
ex-type CPC 27238 = CBS 141301; ITS sequence GenBank KX228278.1,
LSU sequence GenBank KX228329.1, MycoBank MB817041).
Colour illustrations. Forest undergrowth on La Réunion Island; conidiophores and conidia on PNA. Scale bars = 10 µm.
Pedro W. Crous, CBS-KNAW Fungal Biodiversity Centre, P.O. Box 85167, 3508 AD Utrecht, The Netherlands; e-mail: p.crous@cbs.knaw.nl
Michael J. Wingfield, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria,
Pretoria 0002, South Africa; e-mail: mike.wingfield@up.ac.za
Johannes J. Le Roux & David M. Richardson, Centre for Invasion Biology, Department of Botany & Zoology,
Stellenbosch University, Matieland 7602, South Africa; e-mail: jleroux@sun.ac.za & rich@sun.ac.za
Dominique Strasberg, Université de La Réunion, UMR PVBMT, Peuplements Végétaux et Bioagresseurs en Milieu Tropical,
15 avenue René Cassin, CS 93002, 97 744 Saint-Denis Messag. Cedex 9, La Réunion, France; e-mail: dominique.strasberg@univ-reunion.fr
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
370
Persoonia – Volume 36, 2016
Paramycoleptodiscus albizziae
371
Fungal Planet description sheets
Fungal Planet 426 – 4 July 2016
Paramycoleptodiscus Crous & M.J. Wingf., gen. nov.
Etymology. Name reflects a morphological similarity with the genus Myco
leptodiscus.
Classification — Incertae sedis, Acrospermales, Dothideo
mycetes.
Mycelium consisting of hyaline, branched, septate hyphae
that become brown, constricted at septa, forming a cluster of
globose, brown, chlamydospore-like cells that form a stroma;
stroma brown, solitary, globose, but with age joining along the
length of hyphae to become strands of radiating stromata;
stroma forming a sporodochium of densely aggregated, dark
brown, finely roughened conidiogenous cells, subglobose to
slightly ampulliform, containing a single, central phialidic locus,
slightly papillate, with cylindrical collarette. Conidia solitary,
aseptate, hyaline, smooth, granular, falcate, slightly curved,
widest in middle, apex subobtusely rounded, base with welldefined fusarium-like foot cell.
Type species. Paramycoleptodiscus albizziae Crous & M.J. Wingf.
MycoBank MB817042.
Paramycoleptodiscus albizziae Crous & M.J. Wingf., sp. nov.
Etymology. Name refers to Albizzia, the plant genus from which this
fungus was collected.
Mycelium consisting of hyaline, branched, septate, 2–3 µm
diam hyphae that become brown, constricted at septa, up to
6 µm diam, forming a cluster of globose, brown, chlamydosporelike cells up to 8 µm diam, that form a stroma; stroma brown,
up to 100 µm diam, solitary, globose, but with age joining along
the length of hyphae to become strands of radiating stromata;
stroma forming a sporodochium of densely aggregated, dark
brown, finely roughened conidiogenous cells, subglobose to
slightly ampulliform, 7–12 × 5 –12 µm, containing a single,
central phialidic locus, slightly papillate, 2–3.5 µm diam, with
cylindrical collarette, 2–3 µm long. Conidia solitary, aseptate,
hyaline, smooth, granular, falcate, slightly curved, widest in
middle, apex subobtusely rounded, base with well-defined
fusarium-like foot cell, (23–)26–32(–37) × (3–)4 µm (including
foot cell), 1–3 × 1–1.5 µm.
Culture characteristics — Colonies reaching up to 15 mm
diam after 2 wk at 25 °C, with spreading, erumpent surface;
margins smooth, lobate, and sparse to moderate aerial mycelium. On MEA surface and reverse mouse-grey. On OA surface
mouse-grey. On PDA surface mouse-grey, reverse dark mousegrey.
Notes — The LSU sequence of Paramycoleptodiscus albiz
ziae is 95 % (544/572) similar to Mycoleptodiscus terrestris
(type of Mycoleptodiscus; CBS 231.53; GenBank JN711859)
and 94 % (754/800) to Arxiella dolichandrae (CBS 138853; GenBank KP004477.1). No highly similar sequences were obtained
with the ITS sequence. Paramycoleptodiscus is distinct from
Mycoleptodiscus (Sutton 1973) in that the latter has septate
conidia with apical appendages, whereas in Paramycolepto
discus the conidia are aseptate, and have a basal appendage
in the form of a foot cell.
Typus. Malaysia, Sabah, Tawau, on leaves of Albizzia falcataria (Faba
ceae), May 2015, M.J. Wingfield (holotype CBS H-22615, culture ex-type
CPC 27552 = CBS 141320; ITS sequence GenBank KX228279.1, LSU
sequence GenBank KX228330.1, MycoBank MB817043).
Colour illustrations. Stand of Albizzia falcataria trees; conidiomata sporulating on OA, sporodochia, conidiogenous cells and conidia. Scale bar = 10
µm.
Pedro W. Crous & Johannes Z. Groenewald, CBS-KNAW Fungal Biodiversity Centre, P.O. Box 85167, 3508 AD Utrecht, The Netherlands;
e-mail: p.crous@cbs.knaw.nl & e.groenewald@cbs.knaw.nl
Michael J. Wingfield, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria,
Pretoria 0002, South Africa; e-mail: mike.wingfield@up.ac.za
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
372
Persoonia – Volume 36, 2016
Malaysiasca phaii
373
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Fungal Planet 427 – 4 July 2016
Malaysiasca Crous & M.J. Wingf., gen. nov.
Etymology. Name refers to Malaysia, the country where it was collected.
Classification — Incertae sedis, Glomerellales, Sordariomy
cetes.
Stroma brown, globose, erumpent, giving rise to a fascicle of conidiophores. Conidiophores subcylindrical, unbranched, erect,
flexuous, basal cell slightly swollen; thick-walled, finely roughened, granular to slightly guttulate, septate, grey-brown, becoming somewhat pale brown towards slightly tapered apex,
rejuvenating percurrently, with apical conidiogenous cells. Coni
diogenous cells integrated, pale brown, subcylindrical, phialidic,
with periclinal thickening and minute collarette. Conidia solitary,
aggregating in a slimy mass, ellipsoidal to cylindrical-ellipsoid to
somewhat clavate, prominently guttulate, frequently with large
central guttule, apex obtuse, base truncate, scar slightly thickened and darkened, frequently excentric. Ascomata perithecial,
base immersed in substrate, obpyriform, papillate, dark brown
with setae around base of perithecium, pale brown; wall of 3–5
layers of pale brown textura prismatica. Paraphyses persistent,
hyaline, branched, septate, longer than asci. Asci unitunicate,
cylindrical-clavate, short-stipitate, apex truncate with shallow
annulus, 8-spored. Ascospores biseriate in ascus, ellipsoid to
oblong, apiculate at ends, with one end having minute mucoid
cap, hyaline, smooth, frequently with large central guttule, becoming 1-septate and pale brown after discharge.
Type species. Malaysiasca phaii Crous & M.J. Wingf.
MycoBank MB817044.
Malaysiasca phaii Crous & M.J. Wingf., sp. nov.
Etymology. Name refers to Phaius, the plant genus from which this fungus
was collected.
Stroma brown, globose, erumpent, to 150 µm diam, giving rise
to a fascicle of 2–10 conidiophores. Conidiophores subcylindrical, unbranched, erect, flexuous, basal cell slightly swollen,
7–12 µm diam; thick-walled, finely roughened, granular to slightly guttulate, 5–8-septate, grey-brown, becoming somewhat pale
brown towards slightly tapered apex, rejuvenating percurrently,
150–220 × 5–7 µm, with apical conidiogenous cell. Conidiog
enous cells integrated, pale brown, subcylindrical, 55–80 × 6
µm, phialidic, with periclinal thickening and minute collarette,
apex 4–5 µm diam. Conidia solitary, aggregating in a slimy
mass, ellipsoidal to cylindrical-ellipsoid to somewhat clavate,
prominently guttulate, frequently with large central guttule, apex
obtuse, base truncate, scar slightly thickened and darkened,
frequently excentric, 3–4 µm diam, (16–)18–20(–24) × (8–)9–
10(–11) µm. On PNA developing a sexual morph after 4 wk,
and essentially dissolving the cellolytic tissue of the pine needle
in the process. Ascomata perithecial, base immersed in pine
needles, to 250 µm wide, 400 µm tall, obpyriform, papillate, dark
brown with setae around base of perithecium, pale brown; wall
of 3–5 layers of pale brown textura prismatica. Paraphyses persistent, hyaline, branched, septate, longer than asci, 2.5–4 µm
diam. Asci 55–80 × 9–12 µm, unitunicate, cylindrical-clavate,
short-stipitate, apex truncate with shallow annulus, 8-spored.
Ascospores biseriate in ascus, ellipsoid to oblong, apiculate at
ends, with one end having minute mucoid cap, hyaline, smooth,
frequently with large central guttule, becoming 1-septate and
pale brown after discharge, (22–)24–27(–30) × (6–)7(–8) µm.
Culture characteristics — Colonies reaching up to 25 mm
diam after 2 wk at 25 °C, with spreading, flat surface; margins
feathery, lobate, and sparse aerial mycelium. On MEA surface
and reverse pale luteous. On OA surface dark mouse-grey. On
PDA surface and reverse mouse-grey.
Typus. Malaysia, Sabah, Tawau, on leaves of Phaius reflexipetalus (Orchi
daceae), 30 May 2015, M.J. Wingfield (holotype CBS H-22616, culture extype CPC 27548 = CBS 141321; ITS sequence GenBank KX228280.1, LSU
sequence GenBank KX228331.1, actA sequence GenBank KX228359.1,
MycoBank MB817045).
Notes — The LSU sequence of Malaysiasca phaii is 98 %
(784/797) similar to that of Monilochaetes dimorphospora (MUCL
40959; GenBank HQ609480.1), 98 % (783/797) to Monilocha
etes guadalcanalensis (CBS 346.76; GenBank GU180640.1)
and 98 % (782 /797) to Australiasca queenslandica (BRIP
24334c; GenBank HM237323.1). Morphologically, Malaysiasca
resembles the genus Australiasca (Réblová et al. 2011), but
is distinct in that it forms a stroma that gives rise to fascicles
of conidiophores, whereas stromata are absent in Australi
asca. In general the asexual morph is similar to species of
Monilochaetes, and would be difficult to distinguish without the
aid of molecular data.
Colour illustrations. Forest undergrowth in Malaysia; ascomata, ascospores and asci; stroma giving rise to conidiophores, conidiogenous cells and
conidia. Scale bars: ascomata = 250 µm, all others = 10 µm.
Pedro W. Crous & Johannes Z. Groenewald, CBS-KNAW Fungal Biodiversity Centre, P.O. Box 85167, 3508 AD Utrecht, The Netherlands;
e-mail: p.crous@cbs.knaw.nl & e.groenewald@cbs.knaw.nl
Michael J. Wingfield, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria,
Pretoria 0002, South Africa; e-mail: mike.wingfield@up.ac.za
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
374
Persoonia – Volume 36, 2016
Libertasomyces myopori
375
Fungal Planet description sheets
Fungal Planet 428 – 4 July 2016
Libertasomyces Crous & Roets, gen. nov.
Etymology. This fungus was collected on Robben Island off the west
coast of Cape Town, South Africa. The word robben is Dutch for ‘seal’, and
thus ‘seal island’ is where Nelson Mandela, the first democratically elected
President of South Africa, was imprisoned for 18 of the 27 years he served
behind bars before the fall of apartheid. Libertas (Latin) for freedom.
Classification — Incertae sedis, Pleosporales, Dothideomy
cetes.
Conidiomata pycnidial, solitary, dark brown, erumpent, globose,
with central ostiole; wall of 3–6 layers of brown textura angularis.
Conidiophores reduced to conidiogenous cells. Conidiogenous
cells lining the inner cavity, hyaline, smooth, ampulliform to doliiform, phialidic with prominent periclinal thickening. Conidia solitary, hyaline, smooth, granular, thin-walled, ellipsoid, widest in
middle, apex obtuse, base truncate to bluntly rounded, aseptate.
Type species. Libertasomyces myopori Crous & Roets.
MycoBank MB817046.
Libertasomyces myopori Crous & Roets, sp. nov.
Etymology. Name refers to Myoporum, the plant genus from which this
fungus was collected.
Conidiomata (on pine needle agar; PNA) pycnidial, solitary, dark
brown, erumpent, globose, to 300 µm diam, with central ostiole;
wall of 3–6 layers of brown textura angularis. Conidiophores
reduced to conidiogenous cells. Conidiogenous cells lining the
inner cavity, hyaline, smooth, ampulliform to doliiform, phialidic
with prominent periclinal thickening, 3–6 × 3–4 µm. Conidia
solitary, hyaline, smooth, granular, thin-walled, ellipsoid, widest
in middle, apex obtuse, base truncate to bluntly rounded, aseptate, (4–)4.5–5(–6) × (2–)2.5–3(–3.5) µm.
Culture characteristics — Colonies reaching up to 30 mm
diam after 2 wk at 25 °C, with spreading, erumpent surface;
margins smooth, lobate, and moderate aerial mycelium. On
MEA surface dirty white, reverse luteous. On OA surface honey.
On PDA surface buff with patches of isabelline, reverse isabelline to buff.
Notes — On LSU Libertasomyces myopori is 98 % (792/808)
similar to Camarosporium quaternatum (CBS 134.97; GenBank
DQ377883.1), 98 % (791/808) to Leptosphaeria rubefaciens
(CBS 387.80; GenBank JF740311.1), 97 % (787/809) to Pleno
domus visci (CBS 122783; GenBank EU754195.1) and 97 %
(786/808) to Neoplatysporoides aloicola (CPC 24435; GenBank
KR476754.1), and represents another genus being phomalike in morphology. Neoplatysporoides aloicola (CPC 24435;
GenBank KR476719.1) also represents the most similar ITS
sequence at 92 % (534/580). Libertasomyces is morphologically quite distinct from Neoplatysporoides in that conidia remain
hyaline and aseptate, in contrast to those of Neoplatysporoides
that are 0–1-septate, golden brown, and with longitudinal striations (Crous et al. 2015a).
Typus. south africa, Western Cape Province, Robben Island, on twigs
of Myoporum serratum (Myoporaceae), May 2015, P.W. Crous & F. Roets
(holotype CBS H-22617, culture ex-type CPC 27354 = CBS 141302; ITS sequence GenBank KX228281.1, LSU sequence GenBank KX228332.1, MycoBank MB817047).
Colour illustrations. Myoporum serratum growing on Robben Island;
conidiomata sporulating on OA, conidiogenous cells and conidia. Scale bars
= 10 µm.
Pedro W. Crous & Johannes Z. Groenewald, CBS-KNAW Fungal Biodiversity Centre, P.O. Box 85167, 3508 AD Utrecht, The Netherlands;
e-mail: p.crous@cbs.knaw.nl & e.groenewald@cbs.knaw.nl
Francois Roets, Department of Conservation Ecology and Entomology, Stellenbosch University, South Africa; e-mail: fr@sun.ac.za
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
376
Persoonia – Volume 36, 2016
Fusicladium paraamoenum
377
Fungal Planet description sheets
Fungal Planet 429 – 4 July 2016
Fusicladium paraamoenum Crous, Jacq. Edwards & P.W.J. Taylor, sp. nov.
Etymology. Name reflects morphological similarity to Fusicladium amoe
num.
Classification — Sympoventuriaceae, Venturiales, Dothideo
mycetes.
Mycelium consisting of pale brown, smooth, branched, septate, 2.5 – 3.5 µm diam hyphae. Conidiophores erect, solitary, subcylindrical, dark brown, thick-walled, smooth, rarely
branched below, 25–120 × 3–4 µm, 1–7-septate. Conidiog
enous cells integrated, terminal, rarely lateral, 10–30 × 3–4
µm, brown, smooth, with several sympodial denticle-like loci,
1–1.5 µm diam, thickened and darkened. Conidia occurring
in short chains, subcylindrical, pale brown, smooth, guttulate,
(0–)1(–3)-septate, ends obtusely rounded, hila thickened and
darkened, 1.5–2 µm diam, (13–)15–20(–28) × (3–)3.5(–4) µm.
Culture characteristics — Colonies reaching up to 10 mm
diam after 2 wk at 25 °C, with spreading, erumpent surface;
margins smooth, lobate, and moderate aerial mycelium. On
MEA and PDA surface umber, reverse chestnut. On OA surface
umber with chestnut outer margin.
Notes — On ITS Fusicladium paraamoenum is 98 % (560/
574) similar to F. amoenum (CBS 254.95; GenBank EU035425.1)
and 97 % (527/545) to F. intermedium (CBS 110746; GenBank
EU035432.1). The LSU sequence is 99 % (907/909) similar
to F. amoenum (CBS 254.95; GenBank EU035425.1) and
99 % (897/ 909) to F. intermedium (CBS 110746; GenBank
EU035432.1). Conidia of F. paraamoenum are larger than those
of F. amoenum (6–)10.5–12.8(–17.3) × (1.5–)2.4–3(–3.8) µm
(Ho et al. 1999).
Typus. australia, Victoria, Toolangi State Forest, S37°33'25.3" E145°31'
55.9", on leaves of Eucalyptus regnans (Myrtaceae), 9 Nov. 2014, P.W. Crous,
J. Edwards & P.W.J. Taylor (holotype CBS H-22618, culture ex-type CPC
25596 = CBS 141322; ITS sequence GenBank KX228282.1, LSU sequence
GenBank KX228333.1, MycoBank MB817048).
Colour illustrations. Eucalyptus regnans trees at Toolangi State Forest;
conidiophores and conidia on SNA. Scale bars = 10 µm.
Pedro W. Crous & Johannes Z. Groenewald, CBS-KNAW Fungal Biodiversity Centre, P.O. Box 85167, 3508 AD Utrecht, The Netherlands;
e-mail: p.crous@cbs.knaw.nl & e.groenewald@cbs.knaw.nl
Jacqueline Edwards, AgriBio, Centre for AgriBiosciences, Department of Economic Development, Jobs, Transport and Resources,
5 Ring Road, La Trobe University, Bundoora, Victoria 3083, Australia;
e-mail: jacky.edwards@ecodev.vic.gov.au
Paul W.J. Taylor, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Australia; e-mail: paulwjt@unimelb.edu.au
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
378
Persoonia – Volume 36, 2016
Ochroconis dracaenae
Fungal Planet description sheets
379
Fungal Planet 430 – 4 July 2016
Ochroconis dracaenae Crous, sp. nov.
Etymology. Name refers to Dracaena, the plant genus from which this
fungus was collected.
Classification — Sympoventuriaceae, Venturiales, Dothideo
mycetes.
Mycelium consisting of smooth, pale brown, septate, branched,
1.5–3 µm diam hyphae. Conidiophores solitary, erect, brown,
smooth, arising from superficial hyphae, subcylindrical, straight
to geniculous-sinuous, branched below or not, 1–6-septate,
10–30 × 2–3 µm. Conidiogenous cells brown, smooth, terminal
and lateral on conidiophores, 5–15 × 2.5–3 µm, containing
several apical, cylindrical denticles, 1–1.5 × 1 µm. Conidia
solitary, subcylindrical, ends obtuse, pale brown, verruculose,
medianly 1-septate, hilum thickened and darkened, 1 µm diam,
(6.5–)7–9(–10) × (3–)3.5(–4) µm.
Culture characteristics — Colonies reaching up to 20 mm
diam after 2 wk at 25 °C, with spreading erumpent surface; margins smooth, lobate, and moderate aerial mycelium. On MEA
surface isabelline, reverse brown-vinaceous. On OA surface
brown-vinaceous. On PDA surface and reverse isabelline.
Notes — On ITS Ochroconis dracaenae is 96 % (691/719)
similar to O. humicola (UZ1582_14; GenBank KP326578.1)
and 97 % (626/647) to O. musae (CBS 121963; GenBank HQ667535.1). No better matches were obtained with the protein
coding sequences. Ochroconis dracaenae has smaller conidia
than O. humicola (8–20 × 3–5 µm) and O. musae (9.0–13.5 ×
4.8–6.7 μm) (Crous et al. 2014a, Samerpitak et al. 2015).
Typus. usa, Texas, Austin, on leaf spots of Dracaena reflexa (Asparaga
ceae), Aug. 2013, P.W. Crous (holotype CBS H-22619, culture ex-type CPC
26115 = CBS 141323; ITS sequence GenBank KX228283.1, LSU sequence
GenBank KX228334.1, rpb2 sequence GenBank KX228370.1, tef1 sequence
GenBank KX228377.1, MycoBank MB817049).
Colour illustrations. Forest path along walkway in Austin, Texas; conidiogenous cells and conidia on SNA. Scale bars = 10 µm.
Pedro W. Crous & Johannes Z. Groenewald, CBS-KNAW Fungal Biodiversity Centre, P.O. Box 85167, 3508 AD Utrecht, The Netherlands;
e-mail: p.crous@cbs.knaw.nl & e.groenewald@cbs.knaw.nl
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
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Persoonia – Volume 36, 2016
Cytospora tibouchinae
381
Fungal Planet description sheets
Fungal Planet 431 – 4 July 2016
Cytospora tibouchinae Crous & M.J. Wingf., sp. nov.
Etymology. Name refers to Tibouchina, the plant genus from which this
fungus was collected.
Classification — Valsaceae, Diaporthales, Sordariomycetes.
Conidiomatal (on pine needle agar; PNA) stromata up to 500 µm
diam, rosette cytosporoid, subdivided by invaginations, up to six
radially arranged. Conidiophores hyaline, smooth, branched,
0 – 3-septate, 15 – 25 × 2 – 3 µm, embedded in a gelatinous
layer. Conidiogenous cells phialidic, with periclinal thickening,
tapering towards apices, collarettes minute, 8–14 × 1.5–2 µm.
Conidia hyaline, smooth, guttulate, allantoid, aseptate, (3 –)
3.5(–4) × 1(–1.5) µm.
Culture characteristics — Colonies covering dish after 2 wk
at 25 °C, with spreading, flat surface; margins smooth, and with
moderate aerial mycelium. On MEA surface grey-olivaceous,
reverse greyish sepia. On OA surface olivaceous grey. On
PDA surface smoke grey with patches of honey, reverse grey
olivaceous.
Notes — Based on ITS sequence data Cytospora tibou
chinae is 98 % (561/575) similar to Cytospora myrtagena
(CMW4046; GenBank AY347380.1) (Adams et al. 2005,
Rossman et al. 2015). Morphologically, Cytospora tibouchinae
has conidia of similar dimensions, 3–3.5(–4) × 1 μm, but the
two species can be distinguished in that C. myrtagena has
unbranched conidiophores, and shorter conidiogenous cells,
5–7 × 1 µm (Adams et al. 2005).
Typus. france, La Réunion, on stems of Tibouchina semidecandra
(Melastomataceae), 12 Mar. 2015, P.W. Crous & M.J. Wingfield (holotype
CBS H-22620, culture ex-type CPC 26333 = CBS 141324; ITS sequence
GenBank KX228284.1, LSU sequence GenBank KX228335.1, MycoBank
MB817050).
Colour illustrations. Tibouchina semidecandra on La Réunion Island;
conidiomata sporulating on PNA, conidiophores and conidia. Scale bars =
10 µm.
Pedro W. Crous, CBS-KNAW Fungal Biodiversity Centre, P.O. Box 85167, 3508 AD Utrecht, The Netherlands; e-mail: p.crous@cbs.knaw.nl
Michael J. Wingfield, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria,
Pretoria 0002, South Africa; e-mail: mike.wingfield@up.ac.za
Johannes J. Le Roux & David M. Richardson, Centre for Invasion Biology, Department of Botany & Zoology,
Stellenbosch University, Matieland 7602, South Africa; e-mail: jleroux@sun.ac.za & rich@sun.ac.za
Dominique Strasberg, Université de La Réunion, UMR PVBMT, Peuplements Végétaux et Bioagresseurs en Milieu Tropical,
15 avenue René Cassin, CS 93002, 97 744 Saint-Denis Messag. Cedex 9, La Réunion, France; e-mail: dominique.strasberg@univ-reunion.fr
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
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Persoonia – Volume 36, 2016
Neotrimmatostroma paraexcenticum
383
Fungal Planet description sheets
Fungal Planet 432 – 4 July 2016
Neotrimmatostroma paraexcentricum Crous, Jacq. Edwards & Pascoe, sp. nov.
Etymology. Name reflects a morphological similarity to Neotrimmato
stroma excentricum.
Classification — Teratosphaeriaceae, Capnodiales, Dothi
deomycetes.
Leaf spots separate, coalescing with age, medium brown, subcircular with indistinct margins, 2–7 mm diam, amphigenous,
but more prominent on epiphyllous surface. Mycelium immersed, consisting of pale brown, septate, branched, 2–3 µm
diam hyphae. Conidiomata sporodochial, chiefly epiphyllous,
concentrically arranged, dark brown, dry powdery, discrete,
to 400 µm diam. Conidiophores micronematous, branched,
septate, medium brown, smooth, densely aggregated, with
differential thickening of periclinal wall, one side thinner than
the other, to 30 µm tall, 3–4 µm diam. Conidiogenous cells
holothallic, integrated, terminal, doliiform to subcylindrical, 7–10
× 3–4 µm. Conidia in sparsely branched chains, smooth, pale
brown, 4-celled, consisting of upper and lower cells with truncate ends, separated by a thick, dark brown transverse septum,
each primary cell with a smaller, lateral, globose secondary
cell on either side of the primary septum. The two primary cells
together are 9–11 µm diam, the secondary cells 4–5 µm diam.
Culture characteristics — Colonies reaching up to 7 mm
diam after 2 wk at 25 °C, with margins smooth, lobate, and
sparse aerial mycelium. On MEA, PDA and OA surface and
reverse iron-grey.
Notes — On ITS Neotrimmatostroma paraexcentricum is
98 % (467/476) similar to N. excentricum (CBS 121102; GenBank KF901518.1) and on tef1 it is 89 % (331/372) similar to
the same isolate (GenBank KF903123.1). Although morphologically similar, the two species can be distinguished in that the
secondary conidium cells of N. excentricum are 2.5–4.5 µm
diam, thus smaller than those of N. paraexcentricum (Sutton
& Ganapathi 1978, Quaedvlieg et al. 2014).
Typus. australia, Victoria, Phillip Island, Oswin Roberts Reserve, on
leaves of Eucalyptus sp. (Myrtaceae), 8 Nov. 2014, P.W. Crous, J. Edwards
& I.G. Pascoe (holotype CBS H-22621, culture ex-type CPC 25594 = CBS
141325; ITS sequence GenBank KX228285.1, LSU sequence GenBank
KX228336.1, tef1 sequence GenBank KX228378.1, MycoBank MB817051).
Colour illustrations. Symptomatic leaves of Eucalyptus sp.; conidiomata
sporulating on leaf spot, colony on OA, conidiophores and conidia. Scale
bars = 10 µm.
Pedro W. Crous & Johannes Z. Groenewald, CBS-KNAW Fungal Biodiversity Centre, P.O. Box 85167, 3508 AD Utrecht, The Netherlands;
e-mail: p.crous@cbs.knaw.nl & e.groenewald@cbs.knaw.nl
Ian G. Pascoe & Jacqueline Edwards, AgriBio, Centre for AgriBiosciences, Department of Economic Development, Jobs, Transport and Resources,
5 Ring Road, La Trobe University, Bundoora, Victoria 3083 Australia;
e-mail: pascoeig@bigpond.net.au & jacky.edwards@ecodev.vic.gov.au
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
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Persoonia – Volume 36, 2016
Setophoma cyperi
385
Fungal Planet description sheets
Fungal Planet 433 – 4 July 2016
Setophoma cyperi Crous & M.J. Wingf., sp. nov.
Etymology. Name refers to Cyperus, the plant genus from which this
fungus was collected.
Classification — Phaeosphaeriaceae, Pleosporales, Dothi
deomycetes.
Associated with leaf scorch symptoms on Cyperus. Ascomata
immersed on host, erumpent in culture, black, globose, to 200
µm diam, with central ostiole; wall of 3–4 layers of dark brown
textura angularis. Pseudoparaphyses intermingled among asci,
hyaline, septate, branched prominently, constricted at septa,
2 – 4 µm diam. Asci bitunicate with apical chamber, subcylindrical, hyaline, smooth, fasciculate, stipitate, 8-spored,
65–80 × 11–13 µm. Ascospores tri- to multiseriate, fusoid with
subobtusely rounded ends, finely verruculose, red-brown, guttulate, 2-septate, slightly constricted at septa, with central cell
somewhat swollen, (26 –)27–29(–31) × (3.5–)4(–4.5) µm.
Culture characteristics — Colonies reaching up to 30 mm
diam on MEA and OA, 10 mm diam on PDA, after 2 wk at
25 °C, with spreading, erumpent surface; margins feathery,
and moderate aerial mycelium. On MEA, PDA and OA surface
dirty white, reverse dirty white to luteous.
Notes — On LSU Setophoma cyperi is 98 % similar to several genera in Phaeosphaeriaceae, including Setophoma (e.g.
825/844 to S. sacchari CBS 333.39, GenBank GQ387586.1).
De Gruyter et al. (2010) introduced the genus Setophoma to accommodate Pyrenochaeta sacchari, and Phookamsak et al.
(2014) recently reported a sexual morph for the genus in Phaeo
sphaeriaceae.
Typus. south africa, Eastern Cape Province, Haga Haga, on leaves of
Cyperus sphaerocephala (Cyperaceae), Dec. 2014, M.J. Wingfield (holotype
CBS H-22622, culture ex-type CPC 25702 = CBS 141450; ITS sequence
GenBank KX228286.1, LSU sequence GenBank KX228337.1, MycoBank
MB817052).
Colour illustrations. Leaves of Cyperus sphaerocephala; pseudoparaphyses, asci and ascospores. Scale bars = 10 µm.
Pedro W. Crous & Johannes Z. Groenewald, CBS-KNAW Fungal Biodiversity Centre, P.O. Box 85167, 3508 AD Utrecht, The Netherlands;
e-mail: p.crous@cbs.knaw.nl & e.groenewald@cbs.knaw.nl
Michael J. Wingfield, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria,
Pretoria 0002, South Africa; e-mail: mike.wingfield@up.ac.za
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
386
Persoonia – Volume 36, 2016
Lareunionomyces syzygii
387
Fungal Planet description sheets
Fungal Planet 434 – 4 July 2016
Lareunionomyces Crous & M.J. Wingf., gen. nov.
Etymology. Named after the island where this fungus was collected, La
Réunion.
Classification — Incertae sedis, Helotiales, Leotiomycetes.
Mycelium consisting of hyaline, smooth, branched hyphae.
Conidiophores solitary, erect, unbranched, subcylindrical, dark
brown, smooth, septate, thick-walled, basal cell slightly swollen, lacking rhizoids. Penicillate conidiogenous apparatus pale
brown, smooth; primary branches brown, smooth, subcylindrical
to clavate, giving rise to up to several secondary branches,
pale brown, subcylindrical to clavate; tertiary branches pale
brown, giving rise to several phialides. Phialides subulate, pale
brown, flexuous, venter cylindrical, with prominent collarette.
Conidia hyaline, smooth, guttulate, subcylindrical, aseptate,
apex bluntly rounded, base truncate, in short chains that form
slimy spore masses.
Type species. Lareunionomyces syzygii Crous & M.J. Wingf.
MycoBank MB817053.
Lareunionomyces syzygii Crous & M.J. Wingf., sp. nov.
Etymology. Name refers to Syzygium, the plant genus from which this
fungus was collected.
Mycelium consisting of hyaline, smooth, branched, 2–3 µm
diam hyphae. Conidiophores solitary, erect, unbranched, subcylindrical, dark brown, smooth, 3–7-septate, 50–100 × 5–8
µm, thick-walled, basal cell slightly swollen, to 10 µm diam,
lacking rhizoids. Penicillate conidiogenous apparatus pale
brown, smooth; primary branches brown, smooth, subcylindrical to clavate, 8–15 × 4–6 µm, giving rise to up to 8 secondary
branches, pale brown, subcylindrical to clavate, 4–10 × 4–5
µm; tertiary branches pale brown, 4–7 × 3–4 µm, giving rise
to several phialides. Phialides subulate, pale brown, flexuous,
9–12 × 2–2.5 µm, venter cylindrical, with prominent collarette,
4–6 µm long, apex 1.5–2 µm diam. Conidia hyaline, smooth,
guttulate, subcylindrical, aseptate, apex bluntly rounded, base
truncate, (3.5–)4(–5) × (1.5–)2 µm, in short chains that form
slimy spore masses.
Culture characteristics — Colonies reaching up to 8 mm
diam after 2 wk at 25 °C, with spreading, erumpent surface;
margins smooth, lobate, and sparse aerial mycelium. On MEA
surface pale luteous, reverse umber. On PDA and OA surface
and reverse umber.
Notes — Based on its mode of conidiogenesis, Wingfield et
al. (1987) regarded Sporendocladia as separate from Phialo
cephala and Jacobs et al. (2003) showed that this complex is
paraphyletic. Based on identification in the MycoBank nucleotide database the LSU sequence of the present collection is
98.4 % similar to an isolate identified as Sporendocladia foliicola
(CBS 201.95) and it is possible that these two species could be
congeneric. The type of Sporendocladia (S. castanaea) is regarded as synonym of S. fumosa (Wingfield et al. 1987, Crous &
Wingfield 1994) but the culture, CBS 518.93, is not congeneric
with the present collection, and hence a new genus is introduced here to accommodate the present collection. Morphologically, Sporendocladia is similar to Lareunionomyces, except that
the latter genus has a more intricate conidiogenous apparatus,
with numerous tightly aggregated branches and phialides.
Typus. france, La Réunion, on leaves of Syzygium jambos (Myrtaceae),
12 Mar. 2015, P.W. Crous & M.J. Wingfield (holotype CBS H-22623, culture
ex-type CPC 26531 = CBS 141326; ITS sequence GenBank KX228287.1,
LSU sequence GenBank KX228338.1, MycoBank MB817054).
Colour illustrations. Branch of Syzygium jambos; colony on OA, conidiophores and conidia. Scale bars = 10 µm.
Pedro W. Crous, CBS-KNAW Fungal Biodiversity Centre, P.O. Box 85167, 3508 AD Utrecht, The Netherlands; e-mail: p.crous@cbs.knaw.nl
Michael J. Wingfield, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria,
Pretoria 0002, South Africa; e-mail: mike.wingfield@up.ac.za
Johannes J. Le Roux & David M. Richardson, Centre for Invasion Biology, Department of Botany & Zoology,
Stellenbosch University, Matieland 7602, South Africa; e-mail: jleroux@sun.ac.za & rich@sun.ac.za
Dominique Strasberg, Université de La Réunion, UMR PVBMT, Peuplements Végétaux et Bioagresseurs en Milieu Tropical,
15 avenue René Cassin, CS 93002, 97 744 Saint-Denis Messag. Cedex 9, La Réunion, France; e-mail: dominique.strasberg@univ-reunion.fr
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
388
Persoonia – Volume 36, 2016
Parawiesneriomyces syzygii
389
Fungal Planet description sheets
Fungal Planet 435 – 4 July 2016
Parawiesneriomyces Crous & M.J. Wingf., gen. nov.
Etymology. Name reflects a morphological similarity to the genus Wiesne
riomyces.
Classification — Wiesneriomycetaceae, Tubeufiales, Dothi
deomycetes.
Mycelium consisting of brown, finely verruculose, branched,
septate hyphae, giving rise to hyphopodia-like structures,
with several lateral branches, creating a cauliflower-like appearance. Setae loosely associated with sporodochia, erect,
flexuous, base bulbous, lacking rhizoids, apex acute, thickwalled, smooth, granular, dark brown, septate. Conidiomata
sporodochial, solitary, becoming somewhat gregarious in older
cultures, hyaline, becoming pale luteous with age; arising from
a basal stroma of loosely aggregated brown hyphae that give
rise to densely aggregated, hyaline, penicillate conidiophores.
Conidiophores hyaline, smooth, penicillate, septate (constricted
at septa), branched, with several series of branches. Conidi
ogenous cells terminal, clavate, hyaline, smooth, straight to gently curved, polyblastic, with several flat-tipped apical loci. Conidia
solitary, aggregated in mucoid mass, hyaline, smooth, granular, prominently guttulate, subcylindrical, widest in middle with
taper towards both ends that are obtusely rounded, septate,
prominently constricted at septa, joined by a narrow isthmus.
Type species. Parawiesneriomyces syzygii Crous & M.J. Wingf.
MycoBank MB817060.
Parawiesneriomyces syzygii Crous & M.J. Wingf., sp. nov.
Etymology. Name refers to Syzygium, the plant genus from which this
fungus was collected.
Mycelium (on SNA, OA and PNA) consisting of brown, finely
verruculose, branched, septate, 3–5 µm diam hyphae, giving
rise to hyphopodia-like structures, up to 25 µm tall, with several
lateral branches (5–15 µm diam), creating a cauliflower-like
appearance. Setae loosely associated with sporodochia, erect,
flexuous, base bulbous (7–15 µm diam), lacking rhizoids, apex
acute, thick-walled, smooth, granular, dark brown, 8–12-septate
(at times minutely constricted at some septa), 180–300 µm tall.
Conidiomata sporodochial, 80–300 µm diam, solitary, becoming
somewhat gregarious in older cultures, hyaline, becoming pale
luteous with age; arising from a basal stroma of loosely aggregated brown hyphae that give rise to densely aggregated, hyaline, penicillate conidiophores. Conidiophores hyaline, smooth,
penicillate, septate (constricted at septa), branched, 40–80 ×
3–4 µm, with up to three series of branches. Conidiogenous
cells terminal, clavate, hyaline, smooth, straight to gently
curved, 5–8 × 3–4 µm, polyblastic, with several flat-tipped apical loci, 0.5–1 µm diam. Conidia solitary, aggregated in mucoid
mass, hyaline, smooth, granular, prominently guttulate, subcylindrical, widest in middle with taper towards both ends that are
obtusely rounded, (4–)6–7-septate, prominently constricted at
septa, joined by a narrow isthmus, (41–)65–75(–80) × 2(–3.5)
µm, median cells 9–12 µm long, terminal cells 8–10 × 2–3 µm.
Culture characteristics — Colonies reaching up to 60 mm
diam after 3 wk at 25 °C, with spreading, flat surface; margins
smooth, even, and moderate aerial mycelium. On MEA, OA
and PDA surface and reverse mouse-grey with patches of dark
mouse-grey.
Typus. france, La Réunion, on leaves of Syzygium jambos (Myrtaceae),
12 Mar. 2015, P.W. Crous & M.J. Wingfield (holotype CBS H-22630, culture
ex-type CPC 26528 = CBS 141333; ITS sequence GenBank KX228288.1,
LSU sequence GenBank KX228339.1, MycoBank MB817061).
Notes — Wiesneriomycetaceae accommodates two genera, namely Wiesneriomyces and Pseudogliophragma (Suetrong et al. 2014, Pratibha et al. 2015). Based on LSU sequence data, the present isolate is more closely related to
Pseudogliophragma indicum (815/825 (99 %); MTCC 11985;
GenBank KM052851.1) than to Wiesneriomyces conjuncto
sporus (798/823 (97 %); BCC18525; GenBank KJ425450.1).
Morphologically, Parawiesneriomyces closely resembles the
genus Wiesneriomyces, but can be distinguished in that sporodochia are not elevated by a dark pseudoparenchymatous
stalk, but arise flat on the agar surface, and the setae are not
directly linked to sporodochia, but also occur in the absence of
sporodochia. Parawiesneriomyces syzygii differs from W. con
junctosporus (setae up to 650 µm tall, conidia 230–360 µm
long) by having shorter setae and conidia (Kuthubutheen &
Nawawi 1988). Incidentally, both Kuthubutheen & Nawawi
(1988) and Suetrong et al. (2014) regarded the conidial propagules as defined here as chains of individual conidia, whereas
we regard this as a single, multiseptate conidium. This is also
based on the difference in morphology between the median and
end cells of the propagule, and that fact that the conidium does
not readily break into smaller ‘conidia’ with age. Pratibha et al.
(2015) were in agreement with this interpretation and referred
to these propagules as phragmoconidia.
Colour illustrations. Conidiomata sporulating on PNA, seta, cauliflowerlike lateral branch, conidiogenous apparatus and conidia. Scale bars: seta
= 300 µm, all others = 10 µm.
Pedro W. Crous, CBS-KNAW Fungal Biodiversity Centre, P.O. Box 85167, 3508 AD Utrecht, The Netherlands; e-mail: p.crous@cbs.knaw.nl
Michael J. Wingfield, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria,
Pretoria 0002, South Africa; e-mail: mike.wingfield@up.ac.za
Johannes J. Le Roux & David M. Richardson, Centre for Invasion Biology, Department of Botany & Zoology,
Stellenbosch University, Matieland 7602, South Africa; e-mail: jleroux@sun.ac.za & rich@sun.ac.za
Dominique Strasberg, Université de La Réunion, UMR PVBMT, Peuplements Végétaux et Bioagresseurs en Milieu Tropical,
15 avenue René Cassin, CS 93002, 97 744 Saint-Denis Messag. Cedex 9, La Réunion, France; e-mail: dominique.strasberg@univ-reunion.fr
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
390
Persoonia – Volume 36, 2016
Pseudophloeospora eucalyptorum
391
Fungal Planet description sheets
Fungal Planet 436 – 4 July 2016
Pseudophloeospora eucalyptorum Crous, Jacq. Edwards & Pascoe, sp. nov.
Etymology. Name refers to Eucalyptus, the plant genus from which this
fungus was collected.
Classification — Incertae sedis, Xylariales, Sordariomy
cetes.
Leaf spots amphigenous, angular to irregular, medium to dark
brown, 2–7 µm diam with raised border. Conidiomata pycnidial on host, in culture appearing more acervular to even
sporodochial, brown, to 250 µm diam; wall of 3–6 layers of
pale brown textura angularis. Conidiophores lining inner cavity, hyaline, smooth, subcylindrical, branched, 1– 5-septate,
15–60 × 2.5–3.5 µm. Conidiogenous cells terminal and lateral,
hyaline, smooth, tapering towards truncate apex, proliferating
sympodially as well as inconspicuously percurrently at apex,
5–15 × 2–2.5 µm. Conidia hyaline, smooth, filiform, guttulate,
flexuous, subcylindrical, widest in lower third, tapering to an
acutely rounded apex, and truncate base, 1.5 µm diam, 3-septate, (30–)50–67(–75) × 2.5(–3) µm.
Culture characteristics — Colonies reaching up to 30 mm
diam after 2 wk at 25 °C, with spreading, erumpent, folded surface; margins feathery, lobate, and moderate aerial mycelium.
On MEA surface dirty white, with patches of pale mouse grey,
reverse sienna with patches of luteous. On OA surface dirty
white. On PDA surface sienna to luteous, reverse ochreous.
Notes — On ITS Pseudophloeospora eucalyptorum is
98 % (612/625) similar to Pseudophloeospora eucalypti (CBS
128212; GenBank HQ599592). Morphologically, the two species can be distinguished in that on average the conidia of
P. eucalypti are larger, (60–)65–75(–80) × (1.5–)2(–2.5) µm
(Crous et al. 2010b).
Typus. australia, Victoria, near Gurdies Winery, Gurdies-St. Helier Road,
The Gurdies, S38°22'49.8" E145°34'23.4", on leaves of Eucalyptus sp.
(Myrtaceae), 7 Nov. 2014, P.W. Crous, J. Edwards & I.G. Pascoe (holotype
CBS H-22624, culture ex-type CPC 25600 = CBS 141327; ITS sequence
GenBank KX228289.1, LSU sequence GenBank KX228340.1, MycoBank
MB817055).
Colour illustrations. Australian winery; symptomatic Eucalyptus leaf,
colony sporulating on OA, conidiophores and conidia. Scale bar = 10 µm.
Pedro W. Crous & Johannes Z. Groenewald, CBS-KNAW Fungal Biodiversity Centre, P.O. Box 85167, 3508 AD Utrecht, The Netherlands;
e-mail: p.crous@cbs.knaw.nl & e.groenewald@cbs.knaw.nl
Ian G. Pascoe & Jacqueline Edwards, AgriBio Centre for AgriBiosciences, Department of Economic Development, Jobs, Transport and Resources,
5 Ring Road, LaTrobe University, Bundoora, Victoria 3083 Australia;
e-mail: pascoeig@bigpond.net.au & jacky.edwards@ecodev.vic.gov.au
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
392
Persoonia – Volume 36, 2016
Proxipyricularia asari
393
Fungal Planet description sheets
Fungal Planet 437 – 4 July 2016
Proxipyricularia asari Crous & M.J. Wingf., sp. nov.
Etymology. Name refers to Asarum, the plant genus from which this
fungus was collected.
Classification — Pyriculariaceae, Magnaporthales, Sordario
mycetes.
Ascomata separate, immersed, globose, brown, to 200 µm
diam, with central papillate ostiole; wall of 2–4 layers of brown
textura angularis. Hamathecium dissolving upon maturity, with
some cells remaining among asci. Asci unitunicate, hyaline,
smooth, 8-spored, subcylindrical, stipitate, apical mechanism
refractive, but not straining in Meltzer’s, 50–75 × 10–12 µm. As
cospores biseriate, fusoid-ellipsoid, widest in middle, with taper
towards subobtusely rounded ends, slightly curved to straight,
3-septate, pale brown, guttulate, (16–)18–20(–22) × (4–)5 µm.
Conidiophores solitary, erect, straight to flexuous, unbranched,
subcylindrical, brown, smooth, 1–8-septate, 55–200 × 3.5–5
µm. Conidiogenous cells integrated, terminal, apex somewhat
swollen with numerous denticle-like loci, 1–1.5 µm tall and in
diam, slightly thickened and darkened, 25–60 × 3.5–5 µm.
Conidia solitary, pyriform, brown, finely verruculose, guttulate,
granular, apex subobtusely rounded, with or without mucoid
cap, base truncate, hilum 1.5 µm diam, darkened, thickened,
2-septate, (20–)22–24(–26) × (6.5–)7–8 µm.
Culture characteristics — Colonies covering dish after 2 wk
at 25 °C, with moderate aerial mycelium and smooth, even
margins. On MEA surface pale mouse-grey with patches of
dirty white, reverse isabelline with patches of pale luteous. On
OA surface honey with patches of pale mouse grey. On PDA
surface honey, reverse isabelline to honey.
Notes — On LSU Proxipyricularia asari is 99 % (736/740)
similar to species of Proxipyricularia, Neopyricularia and Pyri
cularia. The rpb1 sequence is 92 % (900/978) similar to Pyri
cularia ctenantheicola (GR0001; GenBank KM485098.1) and
89 % (868/975) to Proxipyricularia zingiberis (HYZiM201-0-1;
GenBank KM485091.1), suggesting that this may even represent yet another genus in this complex (Klaubauf et al. 2014).
Typus. Malaysia, Sabah, on leaves and stems of Asarum sp. (Aristolochia
ceae), May 2015, M.J. Wingfield (holotype CBS H-22625, culture ex-type
CPC 27444 = CBS 141328; ITS sequence GenBank KX228291.1, LSU
sequence GenBank KX228342.1, actA sequence GenBank KX228361.1,
rpb1 sequence GenBank KX228368.1, MycoBank MB817056); ibid., CPC
27442 (ITS sequence GenBank KX228290.1, LSU sequence GenBank
KX228341.1, actA sequence GenBank KX228360.1).
Colour illustrations. Forest undergrowth in Malaysia; ascomata on stem
of Asarum sp., ascoma, asci, conidiophores and conidia. Scale bars: ascoma
= 200 µm, all others = 10 µm.
Pedro W. Crous & Johannes Z. Groenewald, CBS-KNAW Fungal Biodiversity Centre, P.O. Box 85167, 3508 AD Utrecht, The Netherlands;
e-mail: p.crous@cbs.knaw.nl & e.groenewald@cbs.knaw.nl
Michael J. Wingfield, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria,
Pretoria 0002, South Africa; e-mail: mike.wingfield@up.ac.za
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
394
Persoonia – Volume 36, 2016
Diaporthe passiloricola
395
Fungal Planet description sheets
Fungal Planet 438 – 4 July 2016
Diaporthe passiloricola Crous & M.J. Wingf., sp. nov.
Etymology. Name refers to Passiflora, the plant genus from which this
fungus was collected.
Classification — Diaporthaceae, Diaporthales, Sordariomy
cetes.
Conidiomata (on pine needle agar; PNA) pycnidial, solitary,
black, erumpent, globose, to 250 µm diam, with short black
neck, exuding creamy droplets from central ostioles; walls
consisting of 3 – 6 layers of medium brown textura angula
ris. Conidiophores hyaline, smooth, 2–3-septate, branched,
densely aggregated, cylindrical, straight to sinuous, 20–50 ×
3–4 µm. Conidiogenous cells 7–20 × 1.5–2.5 µm, phialidic,
cylindrical, terminal and lateral with slight taper towards apex,
1–1.5 µm diam, with visible periclinal thickening; collarette not
observed. Paraphyses not observed. Alpha conidia aseptate,
hyaline, smooth, guttulate, fusoid-ellipsoid, tapering towards
both ends, apex subobtuse, base subtruncate, (5–)6–7(–9) ×
2.5(–3) µm. Gamma conidia not observed. Beta conidia spindleshaped, aseptate, smooth, hyaline, apex acutely rounded,
base truncate, tapering from lower third towards apex, curved,
(20–)22–25(–27) × 1.5(–2) µm.
Culture characteristics — Colonies covering dish after 2 wk
at 25 °C, with even, smooth margins, and fluffy aerial mycelium.
On MEA surface dirty white, reverse luteous to ochreous. On
OA surface dirty white. On PDA surface dirty white, reverse
saffron.
Notes — On ITS Diaporthe passifloricola is 98 % (556/567)
similar to D. miriciae (BRIP 56918a; GenBank KJ197284.1) and
90 % (466/519)–93 % (402/430) similar to five sequences of
‘Phomopsis’ tersa deposited on GenBank (e.g. KF516000.1 and
JQ585648.1). The his3 sequence is 100 % (380/380) identical
to D. absenteum (LC3564; GenBank KP293559.1) and 99 %
(378/380) to the sterile Diaporthe ‘sp. 1 RG-2013’ (LGMF947;
GenBank KC343687.1), whereas the tub2 sequence is 99 %
(513/517) to the sterile Diaporthe ‘sp. 1 RG-2013’ (LGMF947;
GenBank KC344171.1) and 99 % (589 /595) to D. miriciae
(BRIP 56918a; GenBank KJ197264.1). Although alpha conidia
of D. miriciae are similar in size ((6–)7.5(–9) × 2–2.5(–3) μm),
beta conidia are larger (20–35 × 1.0–1.5 μm) and conidiophores are shorter (10–20 × 1.5–3 μm) (Thompson et al. 2015).
Other species previously reported from Passiflora include
D. passiflorae (conidia 14–20 × 1.5–2 μm; Crous et al. 2012a)
and ‘Phomopsis’ tersa (conidia 6.5–7.5 × 2.5 μm) (Sutton 1980).
‘Phomopsis’ tersa has alpha conidia of similar dimensions, but
has much larger conidiomata (to 650 µm diam), shorter conidiophores (to 15 µm long) and lacks beta conidia (Sutton 1980).
Typus. Malaysia, Kota Kinabalu, on leaf spots of Passiflora foetida (Passi
floraceae), May 2015, M.J. Wingfield (holotype CBS H-22626, culture ex-type
CPC 27480 = CBS 141329; ITS sequence GenBank KX228292.1, LSU sequence GenBank KX228343.1, his3 sequence GenBank KX228367.1, tub2
sequence GenBank KX228387.1, MycoBank MB817057).
Colour illustrations. Flower of Passiflora foetida; conidiomata sporulating
on PNA, conidiophores, beta and alpha conidia. Scale bars: conidiomata =
250 µm, all others = 10 µm.
Pedro W. Crous & Johannes Z. Groenewald, CBS-KNAW Fungal Biodiversity Centre, P.O. Box 85167, 3508 AD Utrecht, The Netherlands;
e-mail: p.crous@cbs.knaw.nl & e.groenewald@cbs.knaw.nl
Michael J. Wingfield, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria,
Pretoria 0002, South Africa; e-mail: mike.wingfield@up.ac.za
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
396
Persoonia – Volume 36, 2016
Diaporthe ocoteae
397
Fungal Planet description sheets
Fungal Planet 439 – 4 July 2016
Diaporthe ocoteae Crous & M.J. Wingf., sp. nov.
Etymology. Name refers to Ocotea, the plant genus from which this fungus
was collected.
Classification — Diaporthaceae, Diaporthales, Sordariomy
cetes.
Conidiomata (on pine needle agar; PNA) pycnidial, solitary,
black, erumpent, globose, to 300 µm diam, exuding creamy
droplets from central ostioles; walls consisting of 3–6 layers
of medium brown textura angularis. Conidiophores hyaline,
smooth, 2–3-septate, branched, densely aggregated, cylindrical, straight to sinuous, 15–35 × 2.5–4 µm. Conidiogenous cells
7–15 × 2–3 µm, phialidic, cylindrical, terminal and lateral with
slight taper towards apex, 1.5 µm diam, with visible periclinal
thickening; collarette not flared, up to 1 µm long when present.
Paraphyses cylindrical, hyaline, smooth, 1–6-septate, 30–80
× 2–3 µm. Alpha conidia aseptate, hyaline, smooth, guttulate,
fusoid, tapering towards both ends, apex subobtuse, base subtruncate, (8–)9–10(–13) × (2–)2.5–3 µm. Gamma conidia not
observed. Beta conidia not observed.
Culture characteristics — Colonies covering dish after 2 wk
at 25 °C, with sparse to moderate aerial mycelium. On MEA
surface dirty white with patches of pale mouse grey, reverse
umber with patches of luteous. On OA surface pale luteous with
patches of umber. On PDA surface and reverse pale luteous
with patches of pale mouse-grey.
Notes — Based on LSU sequence data Diaporthe ocoteae
is 99 % similar to species of Diaporthe and Phaeocytostroma
(Lamprecht et al. 2011, Rossman et al. 2015) and based on
ITS < 95 % and tub2 < 90 % similar to presently known species
of Diaporthe. As far as we could establish, no Diaporthe sp.
has thus far been described from Ocotea and hence Diaporthe
ocoteae is introduced as a new species.
Typus. france, La Réunion, on leaves of Ocotea obtusata (Lauraceae),
6 Mar. 2015, P.W. Crous & M.J. Wingfield (holotype CBS H-22627, culture
ex-type CPC 26217 = CBS 141330; ITS sequence GenBank KX228293.1,
LSU sequence GenBank KX228344.1, tub2 sequence GenBank KX228388.1,
MycoBank MB817058).
Colour illustrations. Forest on La Réunion; conidiomata sporulating on
PNA, conidiophores and conidia. Scale bar = 10 µm.
Pedro W. Crous, CBS-KNAW Fungal Biodiversity Centre, P.O. Box 85167, 3508 AD Utrecht, The Netherlands; e-mail: p.crous@cbs.knaw.nl
Michael J. Wingfield, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria,
Pretoria 0002, South Africa; e-mail: mike.wingfield@up.ac.za
Johannes J. Le Roux & David M. Richardson, Centre for Invasion Biology, Department of Botany & Zoology,
Stellenbosch University, Matieland 7602, South Africa; e-mail: jleroux@sun.ac.za & rich@sun.ac.za
Dominique Strasberg, Université de La Réunion, UMR PVBMT, Peuplements Végétaux et Bioagresseurs en Milieu Tropical,
15 avenue René Cassin, CS 93002, 97 744 Saint-Denis Messag. Cedex 9, La Réunion, France; e-mail: dominique.strasberg@univ-reunion.fr
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
398
Persoonia – Volume 36, 2016
Phaeosphaeria breonadiae
399
Fungal Planet description sheets
Fungal Planet 440 – 4 July 2016
Phaeosphaeria breonadiae Crous & Jol. Roux, sp. nov.
Etymology. Name refers to Breonadia, the plant genus from which this
fungus was collected.
Classification — Phaeosphaeriaceae, Pleosporales, Dothi
deomycetes.
Leaf spots prominent on hypophyllous leaf surface, subcircular,
medium brown, erumpent, with raised margin, 3–7 mm diam,
with immersed black pseudothecia; upper leaf surface with
pale green spot, lacking any ascomata. Ascomata globose to
papillate, dark brown with central darker brown ostiole, up to
30 µm diam, solitary, but commonly aggregated in a cluster,
joined by stromatic tissue, up to 250 µm diam; wall of several
layers of brown textura angularis. Asci short stipitate, bitunicate,
cylindrical-ellipsoid, with obtuse apex and small apical chamber, 35–60 × 8–11 µm, 8-spored, straight to irregularly curved.
Pseudoparaphyses intermingled among asci, hyaline, septate,
anastomosing, hyphae-like, 2.5–3.5 µm diam. Ascospores bi- to
triseriate in asci, fusoid-ellipsoidal with obtuse ends, 3-septate,
constricted at median septum, second cell from apex swollen,
medium brown, smooth, guttulate to granular, (16–)18–20(–21)
× (3.5–)4–5 µm; ascospores are homothallic and produce the
sexual morph in culture; ascospores distorting somewhat at
germination, brown, finely roughened, with germ tubes growing
parallel to the long axis of the spore.
Culture characteristics — Colonies reaching up to 40 mm
diam after 2 wk at 25 °C, with spreading, flat surface; margins
uneven, feathery; aerial mycelium absent to sparse. On MEA
surface dirty white to pale luteous, reverse luteous. On OA surface luteous. On PDA surface luteous, reverse saffron.
Notes — At least three species are present on these lesions.
One of these resembled Teratosphaeria, another having aseptate ascospores and the third being the Phaeosphaeria ascomycete described here. LSU sequence data places Phaeosphaeria
breonardiae with 98 % identity in Phaeosphaeria. Based on
ITS, it is 99 % (564/566) similar to ‘Phoma sp. 2’ TMS-2011
voucher SC12d10p12-12 (GenBank HQ631048.1), an isolate
obtained from decaying sugarcane in the USA (Shrestha et
al. 2011). The isolate did not produce the asexual morph in
culture, but based on its DNA sequence, it appears to be the
same, or closely related species to the USA isolate (Shrestha
et al. 2011). The tef1 sequence did not yield results showing
high similarity to other fungi.
Typus. south africa, Limpopo Province, Wolkberg, on leaves of Breonadia
microcephala (Rubiaceae), Jan. 2015, J. Roux (holotype CBS H-22631, culture ex-type CPC 25944 = CBS 141334; ITS sequence GenBank KX228294.1,
LSU sequence GenBank KX228345.1, tef1 sequence GenBank KX228379.1,
MycoBank MB817062).
Colour illustrations. Wolkberg in Limpopo Province; leaf spot, ascomata
on OA, asci and germinating ascospores. Scale bars = 10 µm.
Pedro W. Crous & Johannes Z. Groenewald, CBS-KNAW Fungal Biodiversity Centre, P.O. Box 85167, 3508 AD Utrecht, The Netherlands;
e-mail: p.crous@cbs.knaw.nl & e.groenewald@cbs.knaw.nl
Jolanda Roux, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria 0002, South Africa;
e-mail: jolanda.roux@up.ac.za
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
400
Persoonia – Volume 36, 2016
Alternaria quercicola
401
Fungal Planet description sheets
Fungal Planet 441 – 4 July 2016
Alternaria quercicola Woudenb. & Mirab., sp. nov.
Etymology. Named after the plant genus from which it was isolated,
Quercus.
Classification — Pleosporaceae, Pleosporales, Dothideo
mycetes
Associated with shothole disease symptoms on leaves. Infected
tissues die and cannot expand as the leaf continues to grow,
eventually tearing out, creating the typical disease symptoms.
Primary conidiophores solitary, smooth, straight to slightly
curved, septate, pale brown with a hyaline tip, (16–)21–37(–48)
× 3–4(–5) µm, bearing 0–1 geniculate conidiogenous extensions with darkened conidiogenous loci. Conidia solitary or in
short chains, simple or branched, pale olive-brown, (narrowly) ovoid to obpyriform, primary conidia (25 –)31– 51(– 57) ×
(6–)8–11(–12) µm, with 3–8 transverse septa and occasionally
1 oblique or longitudinal septa, rough walled at the lower sections and smooth walled at the top sections. The conidial body
sometimes constricted near thickened and darkened transverse
septa. Conidia can form an apical secondary conidiophore,
which can again form a geniculate conidiogenous extension.
Sexual morph not observed.
Culture characteristics — After 7 d cultures on SNA flat,
fimbriate, colourless, aerial mycelium sparse, colonies reaching 50 mm diam; cultures on PCA flat, entire, colourless, aerial
mycelium sparse, colonies reaching 60 mm diam.
Notes — Species of Alternaria are commonly associated
with leaf spot, postharvest and other diseases of various crops.
Recent molecular studies have revealed that species of the
genus cluster in several distinct species clades, now referred to
as sections, which places A. quercicola within section Infectoria
(Woudenberg et al. 2013, 2014, 2015). Based on the gene loci
sequenced, A. quercicola is clearly distinct from other taxa in
the Infectoria complex. Alternaria querci represents a potentially
similar species occurring on Fagaceae in China (Zhang et al.
1999). Unfortunately, no culture was available for study, and
the description was insufficient to make a good comparison.
Typus. iran, Fars province, on leaves of Quercus brantii (Fagaceae), 3
May 2013, M. Mirabolfathy (holotype CBS H-22640, culture ex-type CPC
26163 = CBS 141466; ITS sequence GenBank KX228295.1, LSU sequence
GenBank KX228346.1, gapdh sequence GenBank KX228362.1, MycoBank MB817068); ibid., cPc 26164, cPc 26165 (ITS, LSU, gapdh sequences GenBank KX228296.1–KX228297.1, KX228347.1–KX228348.1,
KX228363.1–KX228364.1).
Colour illustrations. Iran, Quercus brantii (Persian oak) tree; conidiophores, with conidiogenous cells and conidia. Scale bars = 10 µm.
Joyce H.C. Woudenberg, Pedro W. Crous & Johannes Z. Groenewald, CBS-KNAW Fungal Biodiversity Centre, P.O. Box 85167,
3508 AD Utrecht, The Netherlands;
e-mail: j.woudenberg@cbs.knaw.nl, p.crous@cbs.knaw.nl & e.groenewald@cbs.knaw.nl
Mansoureh Mirabolfathy, Iranian Research Institute of Plant Protection, Tehran, Iran;
e-mail: mmirab2000@yahoo.com
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
402
Persoonia – Volume 36, 2016
Stemphylium beticola
403
Fungal Planet description sheets
Fungal Planet 442 – 4 July 2016
Stemphylium beticola Woudenb. & Hanse, sp. nov.
Etymology. Named after the plant genus from which it was collected,
Beta.
Classification — Pleosporaceae, Pleosporales, Dothideo
mycetes.
Conidiophores solitary, straight to flexuous, occasionally branched, septate, smooth, pale brown, (41–)45–72(–88) × 4–5 µm,
bearing 1–3 darkened percurrent rejuvenation sites. Conidi
ogenous cells swollen at the apex, darkened, 5–6 µm wide.
Conidia solitary, conidium body pale olive-brown, verrucose,
ellipsoid to cylindrical, (21–)22–26(–30) × (13–)14–16(–18)
µm, L/W = 1.6, with 2–4 transverse septa and 1–3 longitudinal
and 0–2 oblique septa per transverse sector. Constricted at
1–2 darkened transverse septa. Occasionally with an apical
secondary conidiophore. Immature ascomata of sexual morph
observed on agar, pseudothecia globose, ellipsoid or irregular,
single or aggregated, ranging from 100 –300 µm tall.
Culture characteristics — After 7 d cultures on SNA flat,
fimbriate, colourless with abundant black ascomatal initials
in the agar, aerial mycelium is sparse, white, colonies reaching 45–55 mm diam; cultures on PCA flat, entire to undulate,
colourless with abundant black ascomata in the agar, aerial
mycelium sparse, floccose, (greenish) olivaceous; colonies
reaching 50 –60 mm diam.
Typus. netherlands, Noord-Brabant, Langenboom, on leaves of Beta vul
garis (Amaranthaceae), 17 Aug. 2011, P. Wilting (holotype CBS H-22486,
culture ex-type GV11-265a = CBS 141024; ITS sequence GenBank
KU850520, LSU sequence GenBank KX228349.1, gapdh sequence GenBank KU850667, MycoBank MB815876).
Notes — In 2007 a new leaf spot disease was first discovered on sugar beet (Beta vulgaris) in the Netherlands, which
spread rapidly throughout the country in the following years
(Hanse 2013). Currently, the disease has been detected in
Belgium, Denmark, Germany, Poland, Slovakia, Sweden and
the UK (unpubl. data). The disease manifests in June – August
and starts with small, irregular, yellow spots on the leaves of
sugar beet. The yellow spots become necrotic from the centre
of the lesion outwards with tissue turning brown. The spots
spread over all the leaves of the plant, and in case of severe
infestation, heavily infected leaves will die. Due to this loss of
leaves the canopy, and thus sugar yield, declines. A Stemphy
lium sp. was detected as the causative agent and additional
field trials showed that the fungus is hard to control with the
current registered fungicides in the Netherlands (Hanse et al.
2015). Host range tests under climate room conditions showed
that the fungus was not restricted to sugar beet, but could also
infect potato (Solanum tuberosum), white mustard (Sinapsis
alba), red beet (Beta vulgaris), spinach (Spinacia oleracea)
and fat hen (Chenopodium album) (Hanse et al. 2015). Blast
searches of the ITS and gapdh gene sequences give high
similarity hits with isolates CBS 116599 (P107), CBS 135690
(P212) and CBS 133892 (P221), which were regarded a new
Stemphylium species by Inderbitzin et al. (2009). Here we
describe the species as Stemphylium beticola.
Additional specimens examined. netherlands, Drenthe, Eerste Exloërmond, on leaves of Beta vulgaris, 11 Sept. 2012, B. Hanse, GV12-474a1 =
CBS 141026; ITS sequence GenBank KU850522, gapdh sequence GenBank
KU850669; Groningen, Nieuwe Pekela, on leaves of Beta vulgaris, 17 July
2012, J. Lingbeek, GV12-288-2 = CBS 141025; ITS sequence GenBank
KU850521.1, gapdh sequence GenBank KU850668.1.
S. xanthosomatis CBS 116585 T
S. paludiscirpi CBS 109842 T
100
S. triglochinicola CBS 133732
S. trifolii CBS 116580
73
S. sarciniforme CBS 116579
S. loti P385
95
99
S. beticola CBS 133892
99
91
S. beticola CBS 141025
S. beticola CBS 116599
S. beticola CBS 141024 T
S. beticola CBS 136590
S. beticola CBS 141026
0.01
Colour illustrations. The Netherlands, Beta vulgaris field; yellow leaf
spots on Beta vulgaris; ascomata; conidiophores with conidiogenous cells
and conidia. Scale bars: ascomata = 100 µm, others = 10 µm.
Maximum likelihood tree based on a multiple sequence alignment of the ITS and gapdh sequences containing 1 083 characters in total. The analysis was run with RAxML v. 7.2.6 (Stamatakis & Alachiotis 2010) using the GTR+CAT model and
included 500 bootstrap replicates. The tree was rooted to
S. xanthosomatis. Bootstrap support values are indicated at
the nodes.
Joyce H.C. Woudenberg, CBS-KNAW Fungal Biodiversity Centre, P.O. Box 85167, 3508 AD Utrecht, The Netherlands;
e-mail: j.woudenberg@cbs.knaw.nl
Bram Hanse, IRS, P.O. Box 32, 4600 AA Bergen op Zoom, The Netherlands; email: hanse@irs.nl
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
404
Persoonia – Volume 36, 2016
Rasamsonia columbiensis
405
Fungal Planet description sheets
Fungal Planet 443 – 4 July 2016
Rasamsonia columbiensis Jurjević, Hubka & S.W. Peterson, sp. nov.
Etymology. The species is named for the type locality, District of Columbia,
USA.
Classification — Trichocomaceae, Eurotiales, Eurotiomy
cetes.
On MEA: Stipes predominantly arising from aerial hyphae, verrucose, (50 –)100 – 250(– 300) × 2.5 – 4 μm, terminating in appressed biverticillate structures, occasionally monoverticillate
or terverticillate, branches on subterminal and intercalary positions, appearing as separate stipes, short 5–50(–75) × 2.5–4
μm; metulae in terminal whorls of 2–5, verrucose, 8–12(–17)
× 2.5–4.5 μm; phialides with long tapering collula, acerose,
occasionally rough (8–)9–12(–16) × 2.5–3.5(–5) μm; conidia
smooth walled, cylindrical to ovoid, (2.5–)3–4(–7) × 2.5–4.5
μm diam.
Culture characteristics — (in darkness, 25 °C after 7 d):
Colonies on malt extract agar (MEA) 30–31 mm diam, colony
texture velutinous to slightly floccose, mycelium white, sporulation abundant, cream-buff (R30; Ridgway 1912), exudate
clear, small droplets predominate, soluble pigments absent,
reverse cream-buff to chamois (R30). Colonies on Czapek
yeast autolysate agar (CYA) 14–15 mm diam, colony texture
velutinous to slightly floccose, mycelium white, at margins
c. 2–3 mm broad zone of submerged growth, sporulation abundant, conidia en masse cream-buff to chamois (R30), exudate
absent, soluble pigments absent, reverse cream-buff (R30).
Colonies on potato dextrose agar (PDA) 29 – 30 mm diam,
colony texture velutinous to slightly floccose, mycelium white,
sporulation abundant, cream-buff (R30), exudate clear, small
droplets are predominant, soluble pigments absent, reverse
cream-buff (R30). Colonies on oatmeal agar (OA) 19–20 mm
diam, colony texture slightly floccose, mycelium white, at margins c. 4–5 mm broad zone of submerged growth, sporulation
abundant, conidia en masse cream-buff to chamois (R30),
exudate, sparse, no soluble pigments. Colonies on Czapek
yeast agar with 20 % sucrose (CY20S) 14–15 mm diam, colony
texture velutinous, mycelium white, sporulation good, conidia
en masse cream-buff (R30), no exudate or soluble pigments,
reverse uncoloured to cream-buff (R30). Colonies on dichloran
glycerol agar (DG18) 8–9 mm diam, colony texture velutinous
to slightly floccose, mycelium white, at margins c. 3 mm broad
zone of submerged growth sporulation abundant, conidia en
masse cream-buff to chamois (R30), exudate absent, soluble
pigments absent, reverse cartridge buff (R30), cream-buff (R30)
to chamois centrally (R30). Colonies on Czapek yeast autolysate agar with 5 % NaCl 2–3 mm diam, sporulation absent.
Colonies on creatine sucrose agar (CREA) 19–20 mm diam,
moderate to good growth, no acid production, mycelium white,
predominantly submerged, sporulation good at the centre of
the colony. Growth rates at different temperatures: colonies on
CYA/MEA (in mm) 20 °C 9–10/19–20; 30 °C 16–17/43–45;
35 °C 15–17/38–39; 37 °C 15–16/30–31; 41 °C 9–10/19–20;
45 °C no growth. Colonies on MEA at 41 °C radially moderate
deep sulcate, no exudate, reverse chamois to Isabella colour
(R30); on CYA at 41 °C, very poor sporulation, mycelium white,
colony texture funiculose to lightly floccose, exudate absent,
soluble pigments absent.
Typus. usa, Washington DC, air of a hotel conference room, 18 June
2015, Ž. Jurjević (holotype BPI 910043, cultures ex-type CBS 141097 = CCF
5289, ITS and partial LSU sequence GenBank LT548281, β-tubulin sequence
GenBank LT548285, MycoBank MB816869).
Notes — BLAST analysis of the ITS and β-tubulin sequences
of Rasamsonia columbiensis with reference sequences (Houbraken et al. 2013, Tanney & Seifert 2013) gave the closest
match with the ex-type strain of R. brevistipitata CBS 128785 T:
ITS 96 %, β-tubulin 87 %.
Rasamsonia columbiensis fails to grow at 45 °C, while closely
related R. brevistipitata grows. Also R. columbiensis has larger
spores ((2.5–)3–4(–7) × 2.5–4.5 μm) than R. brevistipitata
((2–)2.5–3(–3.5) × 1.7–2.1 μm).
98 [JX272994]
[ JX272968]
R. piperina CBS 104.69
R. piperina CBS 408.73T
[JF417483]
99 [JF417473]
[ JX272988]
100
100 [ JX272980]
[ JF417491]
T548281]
88
R. eburnea CBS 100538T
R. eburnea CBS 124447
R. aegroticola CBS 132819T
R. aegroticola DTO 137B7
R. argillacea CBS 101.69T
R. columbiensis CBS 141097T sp. nov.
[JF417488]
R. brevistipitata CBS 128785T
100 [ JF417489]
R. brevistipitata CBS 128786
100 [KF242514]
Best scoring maximum likelihood tree (T92+G substitution model) based on sequences of the ITS rDNA showing relationships
of Rasamsonia columbiensis to other Rasamsonia species. The
tree was constructed with the IQ-TREE v. 1.4.0 (Nguyen et al.
2015). Dataset contained 21 taxa and a total of 697 characters
with 169 characters variable and 125 parsimony-informative.
Support values at branches were obtained from 1 000 bootstrap
replicates. Only bootstrap support ≥ 70 % are shown on the
branches; ex-type strains are designated by a superscript T.
[KF242515]
100 [JF417470]
[JF417471]
99 [ JF417476]
[ JF417477]
R. pulvericola DAOM 242435T
R. pulvericola DAOM 242436
R. cylindrospora CBS 275.58T
R. cylindrospora CBS 432.62
R. byssochlamydoides CBS 413.71T
R. byssochlamydoides CBS 533.71
100 [JF417478]
[JF417480]
R. emersonii CBS 393.64T
R. emersonii CBS 397.64
[ JF970184]
100 [JQ178360]
R. composticola CGMCC 3.13669T
R. composticola CGMCC 3.14946
[JF417486]
Trichocoma paradoxa CBS 103.73
0.05
Colour illustrations. USA; 7-d-old cultures of Rasamsonia columbiensis
on CYA (25 °C - top) and MEA (25 °C - middle and 41 °C - bottom), conidiophores and conidia on MEA. Scale bars = 10 µm.
Željko Jurjević, EMSL Analytical, Inc., 200 Route 130 North, Cinnaminson, NJ 08077, USA; e-mail: zjurjevic@emsl.com
Vit Hubka, Department of Botany, Faculty of Science, Charles University in Prague, Benátská 2, 12801 Prague 2, Czech Republic;
e-mail: hubka@biomed.cas.cz
Stephen W. Peterson, Mycotoxin Prevention and Applied Microbiology Research Unit, Agricultural Research Service, U.S. Department of Agriculture,
1815 North University Street, Peoria, IL 61604, USA; e-mail: stephen.peterson@ars.usda.gov
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
406
Persoonia – Volume 36, 2016
Microascus longicollis
407
Fungal Planet description sheets
Fungal Planet 444 – 4 July 2016
Microascus longicollis Hubka, Lysková, Cmokova & M. Kolařík, sp. nov.
Etymology. Refers to the long annellate zone.
Classification — Microascaceae, Microascales, Sordario
mycetes.
Ascomata immersed, less commonly superficial, predominantly
formed in the colony centre, globose or subglobose, (60–)80–
230 μm diam, without ostiolar neck, black, glabrous, ripening
after 2–3 weeks of cultivation on OA, ascomata are absent or
develop tardily on other media (3–6 weeks on MEA and PDA),
peridium with a textura angularis. Asci globose, subglobose,
ellipsoidal or pyriform, 10–15(–17.5) × 9–13.5 μm. Ascospores
lemon-shaped, pale brown, 4.5–5.5 × 3–4 μm. Conidiophores
represented by individual conidiogenous cells (annellides) on
the hyphae, sometimes supported by a basal cell of 3.5–5 ×
2 – 3 μm, bearing 1– 4 annellides. Annellides with a swollen
base, 4.5–7(–9) × 2.5–3.5(–4.5) μm, tapering to a cylindrical annellated zone, up to 30 μm long and 1.5–2.5 μm wide.
Conidia 1-celled, thick-walled, hyaline to pale brown, brown in
mass, ovate, pyriform or ellipsoidal, with a rounded or pointed
apex and truncate base, smooth, 3.5–5(–6) × 2.5–3.5 μm.
Chlamydospores globose to ellipsoidal, 5 –10 × 5–6.5 μm.
Culture characteristics — (in the dark, 25 °C after 14 d):
Colonies on OA attained 25–27 mm diam, flat, predominantly
submerged, greyish brown in the centre (ISCC-NBS No. 61)
due to production of black ascomata, reverse greyish brown
(No. 61) in the colony centre. Colonies on PCA attained 24–28
mm diam, flat, predominantly submerged except of granular
central part, dark greyish yellow (No. 91) to pale yellowish green
(No. 121), reverse pale yellowish green (No. 121) to greyish
yellowish green (No. 122) in the colony centre. Colonies on
PDA attained 23–27 mm diam, downy, centrally raised, slightly
radially furrowed, surrounded by 5 mm broad submerged zone,
light greyish olive (No. 109) to greyish yellowish green (No.
122), reverse light greyish olive. Colonies on MEA attained
14–24 mm diam, downy, centrally raised, radially furrowed,
light greyish olive (No. 109) to greyish yellowish green (No.
122), surrounded by 5 mm broad submerged zone, reverse dark
greyish yellow (No. 91) to greyish brown (No. 61). Colonies on
MEA after 7 d at 37 °C and 40 °C attained 12–15 and 4–6 mm
diam, respectively.
Microascus longicollis was associated with a case of suspected
onychomycosis of the great toenail of a 48-yr-old female living
in the Czech Republic. The fungus was isolated in pure culture
from the nail scrapings collected during the first visit and the
direct microscopic examination was positive for hyphae. The
etiological significance of the isolate could not be confirmed
because the second mycological examination (negative) was
performed several months after initiation of the treatment at the
time of significant clinical improvement (naftifine hydrochloride:
2 mo, without effect; changed to cyclopirox olamine: effective).
Repeated isolation of the same non-dermatophyte fungus in
pure culture is required for confirmation of its etiological role
(Summerbell et al. 2005).
Colour illustrations. Czech Republic, toenail with suspected onychomycosis; Colonies, top to bottom: 21-d-old colonies of Microascus longicollis
growing on OA, MEA and PDA at 25 °C; micromorphology, left to right: annellides, conidia (top row), ascospores (bottom row), ascomata, asci. Scale
bars = 10 µm, scale bar of the subfigure with ascomata = 100 µm.
M. paisii CBS 213.27T
M. croci CBS 158.44T
M. hyalinus CBS 766.70T
M. expansus CBS 138127T
M. murinus CBS 830.70T
99
100
M. trigonosporus CBS 218.31T
M. macrosporus CBS 662.71T
M. pyramidus CBS 212.65T
86
M. restrictus CBS 138277T
M. verrucosus CBS 138278T
M. chartarus CBS 294.52T
M. cinereus CBS 138709T
M. terreus CBS 601.67T
M. cirrosus CBS 217.31T
M. gracilis CBS 369.70T
M. alveolaris CBS 139501T
M. senegalensis CBS 277.74T
[LT548287] M. longicollis CBS 141177T sp. nov.
100
99
94
M. chinensis CBS 139628T
M. chinensis BMU01895
99
Typus. czech rePublic, Prague, ex toenails of 48-yr-old female with suspected onychomycosis, 20 Dec. 2013, P. Lysková (holotype PRM 935209,
isotype PRM 935210, culture ex-type CCF 5317 = CBS 141177; ITS and
LSU sequence GenBank LT548275, β-tubulin sequence GenBank LT548282,
TEF1-α sequence GenBank LT548287, MycoBank MB816867).
Notes — The ability of this species to grow at 40 °C, ascomata
without ostiolar neck, lemon-shaped ascospores and annellides
with annellate zone up to 30 μm long make M. longicollis well
distinguishable from all species accepted to date (SandovalDenis et al. 2016, Jagielski et al. 2016).
M. campaniformis CBS 138126T
92
93
100
99
100
M. brunneosporus CBS 138276T
M. onychoides CBS 139629T
M. onychoides BMU03909
M. intricatus CBS 138128T
M. intricatus FMR 12362
M. pseudolongirostris CBS 462.97T
M. longirostris CBS 196.61T
100
Chaetomium madrasense CBS 113.83
Chaetomium cuniculorum CBS 121.57
0.02
Best scoring maximum likelihood tree (GTR+G+I substitution
model) based on sequences of the TEF1-α showing the relationship of M. longicollis to other Microascus species. The tree was
constructed with IQ-TREE v. 1.4.0 (Nguyen et al. 2015). Dataset
contained 30 taxa and a total of 812 characters of which 226
were variable and 166 parsimony-informative. Support values at
branches were obtained from 1 000 bootstrap replicates. Only
bootstrap support ≥ 70 % are shown on the branches; ex-type
strains are designated by a superscript T.
Vit Hubka & Adéla Čmoková, Department of Botany, Faculty of Science, Charles University in Prague, Benátská 2, 128 01 Prague 2, Czech Republic;
e-mail: hubka@biomed.cas.cz & cmokova@gmail.com
Pavlína Lysková, Laboratory of Medical Mycology, Department of Parasitology, Mycology and Mycobacteriology Prague,
Public Health Institute in Usti nad Labem, Sokolovská 60, 186 00 Prague 8, Czech Republic; e-mail: Pavlina.Lyskova@zuusti.cz
Miroslav Kolařík, Laboratory of Fungal Genetics and Metabolism, Institute of Microbiology of the AS CR, v.v.i, Vídeňská 1083,
142 20 Prague 4, Czech Republic; e-mail: mkolarik@biomed.cas.cz
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
408
Persoonia – Volume 36, 2016
Paecilomyces tabacinus
409
Fungal Planet description sheets
Fungal Planet 445 – 4 July 2016
Paecilomyces tabacinus Jurjević, Hubka & S.W. Peterson, sp. nov.
Etymology. Named after the host from which it was isolated, Tobacco.
soluble pigments absent, reverse Isabella colour to brownish olive (R30). Colonies on Czapek yeast agar with 20 % sucrose (CY20S) 9–11 mm diam, sporulation very poor, mycelium
white, submerged, reverse uncoloured. Colonies on Dichloran
glycerol agar (DG18) 8–10 mm diam, sporulation very poor,
mycelium white, submerged, reverse uncoloured. No growth on
CYA with 5 % NaCl. Colonies on Oatmeal agar (OA) 37–39 mm
diam, colony texture floccose, mycelium white, sporulation very
good, conidia en masse light-buff to warm-buff (R15), exudate
absent, soluble pigments absent. Colonies on creatine sucrose
agar (CREA) 40–42 mm diam, poor to moderate growth, no
acid production, mycelium white, colony texture floccose to
submerged into the agar, sporulation poor to good. On MEA
(colony diam in mm) at 30 °C > 90; 35 °C > 90; 37 °C 68–71;
41 °C 18–19; no growth at 45 °C.
Classification — Thermoascaceae, Eurotiales, Eurotiomy
cetes.
On MEA: Stipes short, smooth, rarely finely roughened, 5–30(–50)
× (2.5–)3–4(–4.5) μm diam; branches irregularly verticillate;
phialides 2–5 per branch, cylindrical, 9–14(–28) × 2.5–3.5(–5)
μm diam, tapering abruptly toward a long cylindrical collula, up
to 7 μm long and 1–2 μm diam, occasionally finely roughened,
solitary phialides common, often forming directly on hyphae;
conidia ellipsoidal, fusiform, pyriform (tear-shaped), rarely
subglobose (small conidia), (2.5–)3–7(–11) × 2.5–7 μm diam,
large pyriform conidia are with broad attachment point up to
3 μm diam, chlamydospores thick walled, abundant, single or in
bunch of grapes, yellow-ochre to Dresden-brown (R15; Ridgway
1912), globose to subglobose, occasionally nearly pyriform,
finely roughened in early stages, later becoming very rough to
spiny, (4–)5–8(–10) μm diam. No sexual morph observed after
prolonged cultivation (4 wk) on media listed below.
Culture characteristics — (in darkness, 25 °C after 7 d): Colonies on malt extract agar (MEA) 74–88 mm diam, colony texture floccose, mycelium white to sayal-brown (R29), sporulation
very good, conidia en masse light-buff to warm-buff (R15), exudate absent, soluble pigments absent, reverse buckthorn-brown
to Dresden-brown (R15). Colonies on Czapek yeast autolysate
agar (CYA) 38–40 mm diam, colony texture floccose, mycelium
white, sporulation good, conidia en masse light buff to antimony-yellow (R15), exudate absent, soluble pigments absent,
reverse light buff to antimony-yellow (R15). Colonies on potato
dextrose agar (PDA) 75–78 mm diam, colony texture floccose,
mycelium white to sayal-brown (R29), sporulation very good,
conidia en masse light-buff to warm-buff (R15), exudate absent,
Typus. usa, North Carolina, Durham, tobacco leaves, greenhouse, 5 June
2015, Ž. Jurjević (holotype BPI 910044, cultures ex-type CBS 141098 = CCF
5290, ITS and LSU sequence GenBank LT548280, β-tubulin sequence GenBank LT548286, calmodulin sequence GenBank LT548288, MycoBank
MB816870).
Notes — BLAST searches of the sequences of P. tabacinus
showed highest degree of similarity with Byssochlamys zoller
niae CBS 374.70 T: ITS 97 %, β-tubulin 94 %, calmodulin 95 %.
Another strain with an identical ITS sequence (GenBank GU934506) originated from roots of Salix, Canada (Corredor et
al. 2012).
Paecilomyces tabacinus is distinguished by production of ellipsoidal or fusiform conidia and numerous coarsely roughened
chlamydospores (smooth, finely roughened or absent in the
majority of species). Closely related B. zollerniae also produces
warted chlamydospores but can be differentiated by the production of sexual state in culture and by having smaller conidia.
Best scoring maximum likelihood tree (T92+G substitution model) based on sequences of the ITS rDNA region showing relationship of P. tabacinus to Paecilomyces and Byssochlamys
species belonging to Eurotiales (Samson et al. 2009). The tree
was constructed with IQ-TREE v. 1.4.0 (Nguyen et al. 2015).
Dataset contained 19 taxa and a total of 588 characters with
138 characters variable and 79 parsimony-informative. Support values at branches were obtained from 1 000 bootstrap
replicates. Only bootstrap support ≥ 70 % are shown on the
branches; ex-type strains are designated by a superscript T.
FJ389947
87
FJ389944
CBS 373.70T
CBS 696.95
FJ389934 CBS 100.11T
97 FJ389936
94 FJ389943
CBS
146.48T
B. fulva
CBS 135.62
B. zollerniae CBS 374.70T
GU934506
99
99 LT548280
93
83
EU037050
P. brunneolus CBS 370.70T
EU037055
CBS 102.74T
FJ389930 CBS 338.51
CBS 284.48T
CBS 110429
FJ389924
P. tabacinus sp. nov.
P. formosus CBS 990.73BT
99
FJ389931
OTU960
CBS 141098T
FJ389929
99
96 FJ389932
Byssochlamys lagunculariae
B. nivea
CBS 113245
FJ389933
84
Paecilomyces dactylethromorphus
CBS 251.55
79 FJ389945
72
CBS 323.34T
FJ389951
P. variotii
P. divaricatus
B. verrucosa CBS 605.74T (Thermoascus clade)
!""#$%&'
Sclerocleista ornata NRRL 2291
0.02
Colour illustrations. Tobacco plant; 7-d-old cultures of Paecilomyces
tabacinus on MEA (25 °C - top, 37 °C - middle, 41 °C - bottom), chlamydospores, conidia and conidiophores on MEA. Scale bars = 10 µm.
Željko Jurjević, EMSL Analytical, Inc., 200 Route 130 North, Cinnaminson, NJ 08077, USA; e-mail: zjurjevic@emsl.com
Vit Hubka, Department of Botany, Faculty of Science, Charles University in Prague, Benátská 2, 12801 Prague 2, Czech Republic;
e-mail: hubka@biomed.cas.cz
Stephen W. Peterson, Mycotoxin Prevention and Applied Microbiology Research Unit, Agricultural Research Service, U.S. Department of Agriculture,
1815 North University Street, Peoria, IL 61604, USA; e-mail: stephen.peterson@ars.usda.gov
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
410
Persoonia – Volume 36, 2016
Chrysosporium echinulatum
411
Fungal Planet description sheets
Fungal Planet 446 – 4 July 2016
Chrysosporium echinulatum Hubka, Mallátová, Cmokova & M. Kolařík, sp. nov.
Etymology. Refers to the surface ornamentation of conidia.
Classification — Onygenaceae, Onygenales, Eurotiomy
cetes.
Hyphae hyaline, septate, smooth-walled, 1.5 – 5 μm wide,
straight, sparsely branched. Conidia hyaline, yellowish in mass,
terminal and lateral conidia sessile or on short right-angled side
protrusions (occasionally swollen) of variable length, solitary or
in chains, initially smooth-walled, nearly all becoming echinulate
at maturity, obovoid to clavate, 1-celled, 4.5–7 × 2.5–4 µm.
Intercalary conidia solitary or in short chains, smooth-walled,
barrel-shaped to ellipsoid. Chlamydospores absent. Racquet
hyphae present. Sexual morph not observed.
Culture characteristics — (in the dark, 25 °C after 14 d):
Colonies on potato dextrose agar (PDA) attained 28–45 mm
diam, floccose, flat or with slightly elevated centre, white with
light yellow (NBS-ISCC No. 86) to pale orange yellow (No. 73)
colony centre, reverse moderate orange yellow (No. 71). Colonies on malt extract agar (MEA) attained 33–36 mm diam, morphology similar to PDA with more pronounced submerged
growth at the colony margins. Colonies on phytone yeast extract
agar (PYE) attained 28–37 mm diam, morphology similar to
PDA. Colonies on oatmeal agar (OA) attained 35–45 mm diam,
flat, downy, pale yellowish pink (No. 31), reverse uncoloured.
No growth at 35 °C.
Notes — BLAST analysis with the ITS rDNA region sequence
gave closest hits to C. pannicola CBS 116.63 (98 %, 526/539 bp,
GenBank AJ005368), C. fluviale IMI 378764 T (92 %, 498/540
bp, GenBank AJ005367) and C. minutisporosum CBS 101577 T
(91 %, 468/515 bp, GenBank AJ131689). LSU rDNA showed
99 % similarity (548/555 bp) to Aphanoascus durus CBS 118.85
(GenBank AB075345) and A. terreus CBS 342.64 T (556/564
bp, GenBank KC989709); other species showed 95 % or lower
similarity.
Chrysosporium echinulatum resembles C. pannicola (= C. evol
ceanni ) and can be distinguished by the inability to grow at
35 °C and smaller conidia. Chrysosporium echinulatum was
associated with a case of suspected tinea pedis in a 35-yr-old
woman living in the Czech Republic. The fungus was isolated
in pure culture from the skin scales collected during the first
visit and the direct microscopic examination was positive for
hyphae. The mycological examination was not repeated and
the etiological significance of the fungus is unclear. We believe
that the infection was in fact caused by a dermatophyte, which
was not isolated or overgrown by the Chrysosporium isolate.
Typus. czech rePublic, České Budějovice, ex skin scales from the sole
of the foot of a 35-yr-old woman, 21 Aug. 2012, N. Mallátová (holotype PRM
935095, isotype PRM 935096, culture ex-type CCF 4652 = UAMH 11824
= CBS 141178; ITS and LSU sequence GenBank LT548276, MycoBank
MB816868).
Colour illustrations. Czech Republic; sole of the foot with suspected tinea
pedis. Micromorphology: left to right: conidia sessile or on the short side
protrusions of the hyphae, conidia sometimes forming short chains, conidia
smooth when young, later echinulate. Macromorphology: colony after 14 d
on PDA (28 mm diam). Scale bars = 10 µm.
Vit Hubka & Adéla Čmoková, Department of Botany, Faculty of Science, Charles University in Prague, Benátská 2, 128 01 Prague 2,
Czech Republic; e-mail: hubka@biomed.cas.cz & cmokova@gmail.com
Naďa Mallátová, Laboratory of Mycology and Parasitology, Hospital České Budějovice, B. Němcové 585/54, 370 01 České Budějovice,
Czech Republic; e-mail: mallatova@nemcb.cz
Miroslav Kolařík, Laboratory of Fungal Genetics and Metabolism, Institute of Microbiology of the AS CR, v.v.i, Vídeňská 1083, 142 20 Prague 4,
Czech Republic; e-mail: mkolarik@biomed.cas.cz
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
412
Persoonia – Volume 36, 2016
Scleroderma capeverdeanum
413
Fungal Planet description sheets
Fungal Planet 447 – 4 July 2016
Scleroderma capeverdeanum M.P. Martín, M. Dueñas & Telleria, sp. nov.
Etymology. The name refers to the country where the holotype was collected.
Classification — Sclerodermataceae, Boletales, Agaricomy
cetes.
Macroscopic characteristics — Basidiomes epigeous, depressed globose to subglobose, 0.8–20 mm diam, sessile (all
the sizes from dry specimens); the base attached to the substrate by a tuft of mycelium and rhizomorphs. Peridium thin (up
to 1 mm thick), 2-layered: external layer pale yellowish to yellowish brown (colour 250; Séguy 1936) covered by dark brown
scales (colour 701), very thin in young specimens, leaving the
surface finely areolated; internal layer whitish. Dehiscence by
an irregular and lacerate apical pore. Gleba compact when
young, becoming powdery when old, blue greyish (colour 493)
to grey-violet (colour 660).
Microscopic characteristics — Basidiospores globose, 8.5–
9.5(–10.5) μm diam, including ornamentation, densely echinulate (ornamentation 0.5–1 μm high), dark brown in 5 % KOH.
Outer layer of peridium composed of interwoven hyphae, hyaline to yellowish, 3.5–4 μm diam; the inner layer composed of
interwoven hyphae, hyaline, 3–5 μm diam, with clamp-connections.
Notes — Mature basidiomes of Scleroderma capeverde
anum show a peridium with brown squamules, similar to Scle
roderma verrucosum, a species widely distributed in Azores,
Canaria Islands, Madeira and Morocco (Kreisel 2001), also with
echinulate spores; however, in young specimens, the peridium
is finely areolated as in Scleroderma bovista, but this species
has reticulate spores. Specimens of S. capeverdeanum were
found in the same locality as collection MA-Fungi 87407 of
Scleroderma bovista, as indicated in the additional material
examined. Based on a megablast search of NCBIs GenBank
nucleotide database, the closest hits using ITS sequences of
S. capeverdeanum were two sequences of Chinese specimens
collected under Eucalyptus grandis (GenBank HM237173 and
HM237174), and misidentified as S. polyrhizum and S. auran
tium, respectively. In the ITS analyses S. capeverdeanum
cluster with these two sequences from China, as a sister group
of S. dunensis, a new species described from Brazil (this issue).
Until now, no species of Scleroderma were reported from Cape
Verde.
97
Typus. caPe Verde, Santiago Island, Parque Natural Serra de Malagueta,
Concejo Sta. Catarina, alt. 907 m, N15°10'28" W28°40'37", on a slope
under Furcraea foetida and Lantana camara, 20 Sept. 2010, M.P. Martín
MPM3238 (holotype MA-Fungi 87406, ITS sequence GenBank KU747111,
LSU sequence GenBank KU747110, MycoBank MB816518).
72
100
100
Additional material examined of Scleroderma bovista. caPe Verde, Santiago Island, Parque Natural Serra de Malagueta, Concejo Sta. Catarina,
alt. 914 m, N15°10'28" W28°40'37", on a slope, 20 Sept. 2010, M.P. Martín
MPM3241 (MA-Fungi 87407, ITS sequence GenBank KX017590).
100
S. dunensis sp. nov.
63
100
S. capeverdeanum sp. nov.
100
100
91
50
98
94
100
Strict consensus tree of 100 equally most parsimonious trees
was obtained after heuristic search (PAUP v. 4.0a147) of
ITS nrDNA sequences. The two new Scleroderma species
described in this issue are marked with rectangles: S. cape
verdeanum and S. dunensis (see Fungal Planet 448). New
sequences of S. bovista from Cape Verde, and S. nitidium from
Brazil are marked in bold. The accession number from EMBL/
GenBank or UNITE databases are indicated. Bootstrap values
greater than 50 % are indicated on the branches. As in our
preliminary studies (Phosri et al. 2009, Rusevska et al. 2014),
Pisolithus arhizus was included as outgroup.
94
81
88
96
60
100
100
100
80
HF933235, Macedonia
HF933240, Macedonia
HF933234, Macedonia
HF933236, Macedonia
FM213340, Spain
FM213341, Hungary
MPM3241, Cape Verde
S. bovista
HF933242, Serbia
EU784409, UK
AB211267, Japan
GQ267487, New Zealand
EU819517, USA
AB099901, Japan
S. suthepense
JX205215, Thailand
FM213339, USA
FM213338, USA
EU718121
S. septentrionale
FM213337, USA
FM213342, USA
AY935514, Spain
HF933239, Macedonia
S. meridionale
HF933238 , Macedonia
UFRN-Fungos 1361, Brazil
UFRN-Fungos 1359, Brazil
UFRN-Fungos 2553, Brazil
UFRN-Fungos 2549, Brazil
UFRN-Fungos 2033, Brazil
UFRN-Fungos 2035, Brazil
UFRN-Fungos 2501, Brazil
UFRN-Fungos 2206, Brazil
UFRN-Fungos 2249, Brazil
UFRN-Fungos 2551, Brazil
HM237173, China (S. polyrhizum)
HM237174, China (S. aurantiacum)
MA-Fungi 87406, Cape Verde
JQ639040, China
JQ639041, China
JQ639042, China
JQ639043, China
S. yunnanense
JQ639043, China
JQ639045, China
JQ639046, China
HQ688789, Argentina
S. patagonicum
HQ688788, Argentina
FM213336, Sweden (S. septentrionale)
FM213333, UK
FM213334, UK
FM213335, UK
GQ166907, USA
EU784413, UK
S. citrinum
EU784414, UK
FJ824090, USA
FM213345, USA
FM213344, USA
EU819439, USA
EU819441, USA
S. michiganense
FM213346, USA
FM213347, USA
FM213348, USA
UFRN-Fungos 2034, Brazil
UFRN-Fungos 2219, Brazil
UFRN-Fungos 1759, Brazil
S. nitidum
UFRN-Fungos 2550, Brazil
UFRN-Fungos 2500, Brazil
EU784412, UK
FM213354, Spain
HF93323, Macedonia
HF933232, Macedonia
AJ629886, Spain
S. verrucosum
HF933241, Macedonia
HF933233, Macedonia
EU784415, UK
EU718117
FM213355, USA
FM213343, Thailand
S. cepa
DQ453694, USA
FM213353, USA
FM213352, USA
EU819438, USA
S. areolatum
GQ166910, USA
FM213351, USA
HF933231, Macedonia
KJ740390, Nepal
S. nastii
FJ840461, Burkina Faso (S. verrucosum)
FM213350, USA
S. polyrhizum
FM213349, USA
S. bermudense
EU718118
FJ840449, Burkina Faso
S. dictyosporum
FJ840443, Burkina Faso
FM213364, Thailand
FM213363, Thailand
FM213362, Thailand
FM213361, Thailand
S. sinnamariense
FM213360, Thailand
FM213359, Thailand
FM213358, Thailand
FM213357, Thailand
HQ687222, Thailand
FM213356, Thailand
EU718126
S. xanthochroum
FM213365, Pisolithus arhizus
Colour illustrations. Cape Verde, Parque Natural Serra de Malagueta,
where the species was collected (M.T. Telleria); basidiome (holotype MAFungi 87406), echinulate spores (holotype MA-Fungi 87406). Scale bars:
basidiomata = 0.5 cm; spores = 1 µm.
María P. Martín, Margarita Dueñas & M. Teresa Telleria, Departamento de Micología, Real Jardín Botánico-CSIC,
Plaza de Murillo 2, 28014 Madrid, Spain;
e-mail: maripaz@rjb.csic.es, mduenas@rjb.csic.es & telleria@rjb.csic.es
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
414
Persoonia – Volume 36, 2016
Scleroderma dunensis
Fungal Planet description sheets
415
Fungal Planet 448 – 4 July 2016
Scleroderma dunensis B.D.B. Silva, Sulzbacher, Grebenc, Baseia & M.P. Martín, sp. nov.
Etymology. The name refers to the type locality, Parque Estadual Dunas
do Natal.
Classification — Sclerodermataceae, Boletales, Agaricomy
cetes.
Macroscopic characteristics — Basidiomes epigeous, depressed subglobose to subglobose, 12–17 mm diam, sessile
or shortly pseudostipitate, up to 2 mm (all the sizes from dry
specimens), the base attached to the substrate by a tuft of
mycelium and rhizomorphs. Peridium composed of two layers,
thin, up to 2 mm thick, pale yellowish to yellowish brown (colour
4A2, 4B4; Kornerup & Wanscher 1978), surface partially smooth
towards the base and covered by small and thin scales on top,
dark brown (colour 5D4, 5E4). Dehiscence by an irregular and
lacerate apical pore. Gleba compact when young, becoming
powdery when old, greyish (colour 5E4) to brownish (colour
KW5C4).
Microscopic characteristics — Basidiospores globose to
subglobose, 8.3 –10.7 × 8.0 –10.8 μm diam, including ornamentation, echinulate, composed by dense narrow pyramidal
warts, 0.9–1.3 μm high, dark brown in 5 % KOH. Outer layer of
peridium composed of interwoven hyphae, hyaline to yellowish,
2.5–4.0(–5.0) μm diam; the inner layer composed of interwoven
hyphae, hyaline, 3.0 –5.5 μm diam, with clamp-connections.
Typus. brazil, Rio Grande do Norte, Natal, Parque Estadual Dunas do
Natal, alt. 73 m, S06°22'47" W35°01'40", on soil close to Coccoloba sp., 14
May 2010, B.D.B. Silva & A.G. Leite (holotype UFRN-Fungos 2033, isotype
MA-Fungi 47736, ITS sequence GenBank KU747112, LSU sequence GenBank KU747105, MycoBank MB814792).
Additional material examined. brazil, Rio Grande do Norte, Baia Formosa,
Reserva Particular do Patrimônio Natural Mata Estrela, alt. 52 m, S06°22'79"
W35°01'23" on soil close to Coccoloba sp., 12 June 2010, B.D.B. Silva et al.
(UFRN-Fungos 1359, ITS sequence GenBank KU747113, LSU sequence
GenBank KU747106); ibid., (UFRN-Fungos 1361, ITS sequence GenBank
KU747114, LSU sequence GenBank KU747107); ibid., 19 June 2010, B.D.B.
Silva et al. (UFRN-Fungos 2035, ITS sequence GenBank KU747117); ibid.,
(UFRN-Fungos 2549, ITS sequence GenBank KU747115, LSU sequence
GenBank KU747108); ibid., 14 July 2010, B.D.B. Silva et al. (UFRN-Fungos
2553, ITS sequence GenBank KU747118); Natal, Parque Estadual Dunas
do Natal, alt. 73 m, S06°22'47" W35°01'40" on soil close to Coccoloba sp., 9
July 2013, M.A. Sulzbacher, T. Grebenc (UFRN-Fungos 2501, ITS sequence
GenBank KU747119); ibid., (UFRN-Fungos 2499, ITS sequence GenBank
KU747120); Paraíba, Reserva Biológica Guaribas, alt. 198 m, S06°44'50"
W35°08'40" on soil close to Coccoloba sp., 27 July 2012, B.D.B. Silva et al.
(UFRN-Fungos 2551, ITS sequence GenBank KU747116, LSU sequence
GenBank KU747109); ibid., 30 June 2013, M.A. Sulzbacher (UFRN-Fungos
2206, ITS sequence GenBank KU747121).
Additional material examined of Scleroderma nitidum. brazil, Rio Grande
do Norte, Natal, Parque Estadual Dunas do Natal, alt. 73 m, S06°22'47"
W35°01'40" on sandy soil, 16 June 2010, B.D.B. Silva, D.S. Alfredo, I.G.
Baseia (UFRN-Fungos 2034, ITS sequence GenBank KU759904, LSU
sequence GenBank KU759903); ibid., 14 May 2010, B.D.B. Silva, A.G. Leite
(UFRN-Fungos 2550, ITS sequence GenBank KU759906, LSU sequence
GenBank KU759905); ibid., 24 June 2013, M.A. Sulzbacher (UFRN-Fungos
2219, ITS sequence GenBank KU759908); ibid., 9 July 2013, M.A. Sulzbach
er, T. Grebenc (UFRN-Fungos 2500, ITS sequence GenBank KU759909);
Paraiba, João Pessoa, Campus universitário da Paraíba, 13 July 2012,
M.A. Sulzbacher ECM-Sulzbacher-400 (UFRN-Fungos 1759, ITS sequence
GenBank KU759907).
Notes — Scleroderma dunensis is one of the most common
species occurring on sand dunes from the Parque Estadual
Dunas do Natal, growing usually close to Coccoloba spp.
Several previous reports for this locality consider this species
to be S. nitidum (Gurgel et al. 2008, Sulzbacher et al. 2013).
Scleroderma dunensis resembles S. areolatum, S. nitidum
and S. verrucosum, mainly by echinulate basidiospores and
peridium opening by irregular dehiscence, differing fundamentally by larger basidiomes and spores in S. areolatum (15–30
mm diam; 10–15 μm), S. nitidum (20–25 mm diam; 7–11 μm)
and S. verrucosum (25–30 mm diam; 9–12 μm) (Guzmán et
al. 2013). However, the ITS nrDNA and LSU sequences of
S. dunensis show greater similarity with a species from Cape
Verde (see tree figure in Scleroderma capeverdeanum (= Fungal
Planet 447)) instead of S. areolatum, S. nitidum or S. verru
cosum.
Colour illustrations. Brazil, Parque Estadual Dunas do Natal, Coccoloba
sp. growing next to the locality where the type species was collected;
a. peridium (holotype UFRN-Fungos 2033), details of the scales on top;
b. basidiome (UFRN-Fungos 2033); c. cross section showing gleba (UFRNFungos 2033); d. echinulate spores (UFRN-Fungos 2035). Scale bars: a =
1 mm; b–c = 2 mm; d = 2 µm.
Bianca D.B. Silva & Iuri G. Baseia, Departamento de Botânica e Zoologia, Universidade Federal do Rio Grande do Norte,
Natal, Rio Grande do Norte, Brazil;
e-mail: biancadeni@yahoo.com.br & iuri.baseia@gmail.com
Marcelo A. Sulzbacher, Departamento de Solos, Universidade Federal de Santa Maria, CCR, Campus Universitário, 971050-900,
Santa Maria, Rio Grande do Sul, Brazil;
e-mail: marcelo_sulzbacher@yahoo.com.br
Tine Grebenc, Slovenian Forestry Institute Vecna pot 2, Ljubljana, Slovenia;
e-mail: tine.grebenc@gozdis.si
María P. Martín, Departamento de Micología, Real Jardín Botánico-CSIC, Plaza de Murillo 2, 28014 Madrid, Spain;
e-mail: maripaz@rjb.csic.es
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
416
Persoonia – Volume 36, 2016
Ganoderma mbrekobenum
417
Fungal Planet description sheets
Fungal Planet 449 – 4 July 2016
Ganoderma mbrekobenum E.C. Otto, Blanchette, Held, C.W. Barnes & Obodai, sp. nov.
Etymology. Named after the Ghanaian Twi word ‘mbrekoben’, which
translates to reddish brown mushroom.
Classification — Ganodermataceae, Polyporales, Agarico
mycetes.
Mature basidiomata annual, pileate, stipitate, dimidiate, applanate, woody to corky when dried, homogenous context
structure, pileus maroon to liver brown when dry, surface hard
and glabrous, margin rounded, thickened, maroon to liver brown
when dry. Stipe substibe (> 5 cm), lateral, columnar, with one
solitary column, maroon; borders with hymenophore thickened.
Pore surface smooth, creamy to snuff brown when dry, pores
4–6 per mm, round to somewhat irregular and slightly elongated, 105–247 × 76–207 µm (av. 167.2 × 123.8 µm; SD 32,
26; n = 100), dissepiments 44–152 µm (av. 83.6 µm; SD 23;
n = 100); tubes 0.1–0.7 mm long, dark brown. Hyphal system
dimitic; generative hyphae slightly inconspicuous, branched,
thin-walled and hyaline; skeletal hyphae most prevalent in the
basidiocarp, occasionally branched, pale to dark brown, 2.5–7
µm thick, tapering towards the end. Basidia not observed.
Basidiospores brown, ovoid to broadly ellipsoid with a truncate
base, bitunicate, verruculose, 8–11.5 × 6–8 µm (av. 10.4 × 7.1
µm; SD 0.7, 0.4; n = 100), perisporium thin, smooth; exosporium
with intermediate thick inter-walled pillars; endosporium thick,
dark brown. Chlamydospores not observed.
Culture characteristics — No live culture obtained.
Notes — Ganoderma mbrekobenum causes decay in the
roots and trunks of angiosperm trees in the southern regions
of Ghana. Sequences were downloaded from GenBank for
phylogenetic analysis with G. mbrekobenum sequences using
the program Geneious R9 (http://www.geneious.com, Kearse et
al. 2012). The complete ITS sequence of the G. mbrekobenum
holotype was used for the Blastn search. The results gave the
highest score to an isolate Ganoderma sp. (EGDA, GenBank
LN774971) from Egypt, with a single nucleotide difference. The
next 14 Blastn hits were to Ganoderma sp. sequences from
a single institution in India. The analysis included only the top
three of these sequences, having four to six differences from
the G. mbrekobenum holotype. A few isolated sequences with
various Ganoderma species names had relatively high Blastn
scores, but were excluded from the analysis because they did
not align with their respective species and are likely G. mbre
kobenum, or closely related. The closest legitimate Ganoderma
species were G. applanatum and G. fornicatum, both with
94 % identity. Additional sequences of other recently described
Ganoderma species from Africa (Coetzee et al. 2015, Crous et
al. 2015b) were included in the analysis. The final alignment
was edited by hand for alignment errors.
Typus. Ghana, Brong Ahafo and Greater Accra Regions, on angiosperms,
June 2015, M. Obodai (holotype MIN 850481, paratype MIN 850482, holotype
ITS sequence GenBank KX000896, LSU sequence GenBank KX000897;
paratype ITS sequence GenBank KX000898, LSU sequence GenBank
KX000899, holotype MycoBank MB816172).
The phylogenetic tree with G. mbrekobenum was constructed
using the Maximum Likelihood plugin PHYML in Geneious R9
(http://www.geneious.com, Kearse et al. 2012), and the substitution model determined by jModelTest (Posada 2008) according to Corrected Akaike Information Criterion (AICc). Gano
derma enigmatica (GenBank KR183855 and KR150678) is the
outgroup. Bootstrap support values ≥ 50 % are given above
branches. The phylogenetic position of G. mbrekobenum is
indicated in bold. The Ganoderma species is followed by the
sample ID and the three letter United Nations country code,
in order of appearance ZAF: South Africa, GHA: Ghana, EGY:
Egypt, IND: India, MYS: Malaysia, CHN: China, USA: United
States.
Colour illustrations. Ghana, Brong Ahafo Region, native tree species
along the road of the Ayum forest (background); basidiocarp in the field with
basidiospores covering the pileus, basidiocarp in lab with basidiospores
cleaned off; skeletal hyphae, basidiospores by light microscopy and SEM.
Scale bars = 3 cm (basidiocarps), 10 µm (microscopic structures).
Eric C. Otto, Robert A. Blanchette & Benjamin W. Held, Department of Plant Pathology, University of Minnesota,
495 Borlaug Hall, 1991 Upper Buford Circle, St. Paul, MN 55108, USA;
e-mail: ottox136@umn.edu, robertb@umn.edu & bheld@umn.edu
Charles W. Barnes, Departamento Nacional de Protección Vegetal, Estación Experimental Santa Catalina,
Instituto Nacional de Investigaciones Agropecuarias, Panamericana Sur Km. 1 vía Tambillo, Cantón Mejía,
Provincia de Pichincha, Quito, Ecuador; e-mail: cbarnes333b@gmail.com
Mary Obodai, CSIR-Food Research Institute, P.O. Box M20, Accra, Ghana; obodaime@yahoo.com
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
418
Persoonia – Volume 36, 2016
Geoglossum raitviirii
419
Fungal Planet description sheets
Fungal Planet 450 – 4 July 2016
Geoglossum raitviirii Fedosova & E.S. Popov, sp. nov.
Etymology. Named after the Estonian mycologist Ain Raitviir, in recognition of his contribution to the study of the Geoglossaceae from the Russian
Far East.
Classification — Geoglossaceae, Geoglossales, Geoglosso
mycetes.
Ascocarps scattered to gregarious, clavate, stipitate, 2.2–4.5
cm tall, dark brown. Ascigerous part clavate, broadly clavate,
1/4–1/2 of the total ascocarp length, 0.5–1.8 cm long, black
to dark brown, darker than the stipe, compressed, dumbbellshaped or oval in cross section, sharply delimited from the stipe
in fresh condition, in herbarium material smooth, ceraceouspubescent due to prominent paraphyses tips extending from the
hymenium. Stipe terete, compressed, brown, in fresh conditions
lighter than the ascigerous part, dark brown and concolorous in
herbarium material, rough, squamous. Asci clavate to broadly
clavate, (161.5–)172.5–176.5(–191.5) × (21–)24.5–26.5(–31)
μm (measured in KOH), Q = (5.5–)6.5–7(–8), 8-spored, with
euamyloid apical ring and inamyloid wall in MLZ and IKI. Asco
spores elongate-clavate, subfusiform to fusiform, narrowed to
basal end, sometimes slightly curved, (49–)76.5–81.5(–93.5)
× (5.5 –) 6 – 6.5(– 9.5) μm (in KOH), Q = (7.5 –)12.5(–15),
brown, sometimes hyaline with pigmented septae and poles,
predominantly 7-septate, rarely with 3–6(–11) septa, with one
or several large oil drops in each cell. Paraphyses straight,
sometimes branched, sparsely or moderately septate, nonagglutinated, (2–)3(–5) μm diam, apically straight, declinate,
circinate or coiled, pale brown. Apical cells of paraphyses
swollen at tips, pyriform, globose, hockey stick-like, hook-like,
cylindrical, sometimes proliferating, (12.5–)32.5–38.5(–97) ×
(4.5–)8.5–9.5(–13.5) μm, pale brown, incrusted. Hyphae of the
stipe surface straight, moderately septate, formed by chains of
several pale brown cells, apical cells clavate.
Habit, Habitat & Distribution — In small groups on soil in
broadleaf forests. The species is known only from two localities
in Primorye, Russia.
spores, and long, swollen apical cells of the paraphyses. Among
other species of Geoglossum with 7-septate ascospores it is
most similar to G. chamaecyparinum, G. variabilisporum, and
G. lineare. Geoglossum chamaecyparinum (Arauzo & Iglesias
2014) shares similar morphology with G. raitviirii, but differs
in more narrow and long asci ((164.5 –)181.5 –196(– 217) ×
(16–)19.5–23(–25.5) μm) and more narrow ascospores ((50–)
72–82(–90) × (5–)6(–6.5) μm). Geoglossum variabilisporum
can be recognised by smaller ascocarps (0.6–1.9 cm), narrower
and shorter asci ((124 –)145.5 –169(–187) × (15.5 –)18.5 –
21(–24) μm) and by presence of the considerable number of
ascospores with more than 7 septa (Arauzo & Iglesias 2014).
Geoglossum chamaecyparinum and G. variabilisporum are
known from Spain, where they were found on sandy soil under
Chamaecyparis lawsoniana. Geoglossum lineare described
from pasture land in Sweden can be separated from G. raitviirii
by the smooth viscid stipe of ascocarps, stout straight paraphyses, smaller asci (140–155 × 13–16 μm) and 3–7-septate
ascospores (45 –65 × 4.5–5.5 μm) (Hakelier 1967).
Typus. russia, Primorsky Kray, Terneysky District, Sikhote-Alin Nature
Reserve, right side of the Zabolochennaya River, the road from Maysa ranger
station to Ust-Shanduy ranger station, N45°14'19" E136°30'40", broadleaf
forest (with Quercus mongolica, Tilia sp., Acer mono, Aralia elata, Betula sp.,
Eleutherococcus senticosus, Corylus mandshurica), on sandy soil, 22 Aug.
2013, A. Fedosova (holotype LE303983, ITS sequence GenBank KT936308,
LSU sequence GenBank KU986891, MycoBank MB814833).
Additional specimen examined. russia, Primorsky Kray, Khasansky District, Kedrovaya Pad Nature Reserve, valley of the Kedrovaya River, vicinity
of the main reserve station, broadleaf forest, on soil along a brook, 19 Aug.
2005, E. Popov (LE291814, ITS sequence GenBank KT936309).
Notes — Geoglossum raitviirii is characterised by medium
sized brown ascocarps with squamous or granulose stipes,
which are normally lighter than the ascigerous part, relatively
short and broad, 8-spored asci, predominantly 7-septate asco-
Colour illustrations. Primorsky Kray, Terneysky District, Sikhote-Alin
Nature Reserve; spores, apical cells of paraphyses, amyloid reaction of
the ascal apical ring, asci, hyphae of the stipe surface, ascocarps (all from
holotype), type locality. Scale bars = 1 cm (ascocarps), 10 µm (microscopic
structures).
Maximum likelihood tree (RAxML web server) was obtained from
the nrITS dataset sequences of Geoglossum raitviirii (H: holotype; P: paratype) and closely related species (TreeBASE submission ID S19197). The Bayesian analysis (MrBayes v. 3.2.5)
was performed under a GTG+G+I model for 1 M generations.
Numbers at branches indicate Maximum likelihood bootstrap
values ≥ 75 % and Bayesian posterior probabilities ≥ 0.95. The
scale bar represents the number of nucleotide changes per site.
Anna G. Fedosova & Eugene S. Popov, Laboratory of Systematics and Geography of Fungi, Komarov Botanical Institute of the
Russian Academy of Sciences, 197376, 2 Prof Popov Str., Saint Petersburg, Russia;
e-mail: anna.fedosova@gmail.com & EPopov@binran.ru
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
420
Persoonia – Volume 36, 2016
Toxicocladosporium hominis
Fungal Planet description sheets
421
Fungal Planet 451 – 4 July 2016
Toxicocladosporium hominis Sandoval-Denis, Gené & Deanna A. Sutton, sp. nov.
Etymology. Referring to the isolation source of the ex-type strain.
Classification — Cladosporiaceae, Capnodiales, Dothideo
mycetes.
Colonies sporulating on synthetic nutrient-poor agar. Mycelium
branched, septate, smooth, subhyaline to pale brown, hyphae
1.5–3 μm wide. Conidiophores simple or branched, subcylindrical, erect, thickening toward the apex, dark brown, smoothand thick-walled, 70 –113 × 3 – 3.5 μm. Conidiogenous cells
integrated, polyblastic, terminal, geniculate, dark brown, 13–30
× 3–4 μm; scars truncate, thickened and darkened, 1.5–2 μm
wide. Primary ramoconidia cylindrical, dark brown, smoothand thick-walled, 15–32 × 2–4 μm, 0–2-septate. Secondary
ramoconidia subcylindrical to cylindrical, pale to dark brown,
smooth- and thick-walled, 11–15 × 2.5 – 4 μm, 0 –1-septate,
sometimes constricted at the septum, giving rise to branched
conidial chains; scars darkened, thickened, 0.5–1.5 μm diam.
Intercalary conidia subcylindrical, brown, smooth- and thickwalled, 9–16 × 3–4 μm, 0–1-septate, usually constricted at
the septum. Small terminal conidia ellipsoidal to clavate, brown,
smooth-walled, 5.5 –8 × 2.5–3.5 μm.
Culture characteristics — Colonies on PDA at 25 °C attaining 13–18 mm diam after 14 d, deep green to dark green
(30D3/F8) (Kornerup & Wanscher 1978), erumpent and folded,
velvety; reverse olive-brown (4D4/4D6). On SNA at 25 °C attaining 8–10 mm diam after 14 d, olive-brown (4E5/E8), flat to
slightly umbonate, velvety to dusty; reverse olive-brown (4F6).
On OA at 25 °C attaining 9 –12 mm diam after 14 d, olivebrown (4E4/4D3), flat, velvety to dusty; reverse olive-brown
(4E4/4D3).
Notes — The genus Toxicocladosporium, typified by T. irri
tans, currently includes 12 species. Segregated from Clado
sporium, Toxicocladosporium differs in the presence of conspicuous, dark septa in the conidiophores and conidia, and
by having flat, thickened and refractive conidiogenous scars
in contrast to the coronate scars of Cladosporium (Crous et
al. 2007b). Toxicocladosporium hominis is phylogenetically
related and morphologically similar to T. strelitziae (Crous et al.
2012b), from which it differs in the production of larger conidiogenous cells (13–30 × 3–4 μm) and intercalary conidia (9–16
× 3–4 μm) vs 10–15 × 2.5–3.5 μm and 10–12 × 2–2.5 μm,
respectively, in T. strelitziae. In addition, the latter species has
smooth to verruculose ramoconidia, secondary ramoconidia
and intercalary conidia, without constrictions in the medial
portion or at the septum.
Based on a megablast search of NCBIs GenBank nucleotide
database, the closest hit using the ITS sequence is T. strelit
ziae CBS 132535 (GenBank KM816684; Identities = 513/522
(98 %), Gaps = 2/522 (0 %)), followed by T. irritans CBS 185.58
(GenBank EU040243; Identities = 510/ 526 (97 %), Gaps =
4 / 526 (0 %)) and T. pini CPC 23639 (GenBank KJ869160;
Identities = 505/527 (96 %), Gaps= 6/527 (1 %)). Closest hits
using the LSU sequence were to T. strelitziae CBS 132535
(GenBank NG042687; Identities = 542/547 (99 %), Gaps =
2 / 547 (0 %)), T. irritans CBS 185.58 (GenBank EU040243;
Identities = 542/547 (99 %), Gaps = 2 /547 (0 %)) and Clado
sporium sp. ATCC 28310 (GenBank KP780464; Identities =
539 /547 (99 %), Gaps = 2/547 (0 %)).
Typus. USA, Florida, Daytona Beach, from human broncoalveolar lavage
fluid, D.A. Sutton (holotype FMR H-13297, isotype deposited at CBS, cultures ex-type FMR 13297 = UTHSCSA DI-13-172 = CBS 140694, ITS
sequence GenBank LN834444, LSU sequence GenBank LN834448, MycoBank MB814942).
Colour illustrations. USA, Florida, view of Daytona Beach (image credit:
First Glow by Kaitlynne-Rae Landry); colony on PDA after 14 d at 25 °C,
conidiophores, conidiogenous cell bearing conidia, ramoconidia, intercalary
and terminal conidia. Scale bars = 5 µm.
Marcelo Sandoval-Denis, Josepa Gené & Josep Guarro, Mycology Unit, Medical School and IISPV, Universitat Rovira i Virgili (URV), Sant Llorenç 21,
43201 Reus, Tarragona, Spain; e-mail: msandovaldenis@gmail.com, josepa.gene@urv.cat & josep.guarro@urv.cat.
Deanna A. Sutton & Nathan P. Wiederhold, Fungus Testing Laboratory, Department of Pathology, University of Texas Health Science Center,
7703 Floyd Curl Dr., San Antonio, Texas 78229-3900, USA; e-mail: suttond@uthscsa.edu & wiederholdn@uthscsa.edu
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
422
Persoonia – Volume 36, 2016
Pisolithus aureosericeus
423
Fungal Planet description sheets
Fungal Planet 452 – 4 July 2016
Pisolithus aureosericeus M.P. Martín, Kaewgrajang, Phosri & Watling, sp. nov.
Etymology. From Latin aureus and sericeus, referring to the colour and
texture of the peridium.
Classification — Sclerodermataceae, Boletales, Agaricomy
cetes.
Macroscopic characteristics — Basidiomes subglobose to
broadly ellipsoid, gasterocarp, 10–50 mm, sessile. Peridium
surface slightly velvety, golden yellow at first, later buff to snuff
brown. Rhizomorphs at the base, small, 0.8 –1.3 mm high
× 0.3 – 0.5 diam. Gleba orange-brown become ferruginous
powdery mass at maturity by the breakdown of the peridioles.
Peridioles subglobose to broadly ellipsoid, 0.2–0.8 × 1.0–1.2
mm diam, thin-walled, surface smooth, bright yellow or greenish
yellow, later a snuff-brown powdery mass when they mature.
Microscopic characteristics — Constituent hyphae intertwined, cream to ochraceous, thin-walled, 2–3.5 mm broad,
septate without encrustation, clamp-connections present. Ba
sidia not seen. Spores globose to subglobose, 8.5–10 × 8–11
µm excluding ornamentation, pale brown, densely ornamented
with pyramidal spines (0.5 –0.8 µm long).
Typus. thailand, Nakhon Ratchasima, alt. 470 m, N14°29'59" E101°56'22",
on clay loam soil, under Hopea odorata trees, 8 Aug. 2012, T. Kaewgrajang
KUFF001 (holotype Herbarium Kasetsart University, ITS sequence GenBank
KU351837, MycoBank MB851695).
Additional materials examined. thailand, Nakhon Ratchasima, alt. 470 m,
N14°29'59" E101°56'22", on clay loam soil, under Hopea odorata trees, 8 Aug.
2012, T. Kaewgrajang KUFF002 (Herbarium Kasetsart University, ITS sequences GenBank KU351835, KU351836); ibid., T. Kaewgrajang KUFF003
(Herbarium Kasetsart University, ITS sequences GenBank KU351838, KU351839, KU351840).
Notes — The genus Pisolithus has for a long time been
considered a genus of species with mainly a xerophytic lifestrategy, being found in shrub-land, woodland-clearings, even
wasteland, and all generally on highly mineral soils (Pilát 1958).
For many years the dark coloured, elongated, narrow stemmed
Pisolithus kisslingii was perhaps the only species linked to
tropical areas being described from the rain forest in Sumatra
(Fischer 1906); although, specimens of this genus had been
located amongst the dried collections of the late John H. Corner
now housed in the herbarium of the Royal Botanic Garden,
Edinburgh. However, after intensive work in the last few years
in more tropical plant-communities has demonstrated a wealth
of species of Pisolithus present certainly in South-East Asia.
The present study delimits a further species, similar in colour
to P. aurantioscabrosus, but differing markedly in the smoother
and slightly velvety outer surface of the exoperidium in contrast
to the erect squamules of the former. Phylogenetic analyses
(parsimony), based on three collections (six specimens /six
sequences) of P. aurosericeus, and previously published data,
mainly from Martin et al. (2002), Phosri et al. (2012), Martín et al.
Colour illustrations. Thailand, Nakhon Ratchasima (T. Kaewgrajang);
a. basidiome (KUFF001); b. basidiomes (KUFF001) detail to shown the
peridioles; c, d. spores (KUFF001). Scale bars = 10 mm (basidiomes), 1 μm
(spores).
(2013) clearly grouped the new sequences with species of
P. aurantioscabrosus from Malaysia collected under Shorea
macropera. However, the specimens of P. aureosericeus form
a cluster together as a group of their own, and were collected
under Hopea odorata. Moreover, the peridium surface is slightly
velvety and golden yellow, and the basidiospores are strongly
ornamented with wedge-shaped extensions giving a very rough
appearance; although Pisolithus spores are ornamented this
present feature is rather uncommon in the genus and helps to
delimit this new taxon under the microscope.
KUFF002 (1), KU351835, P
Clade 15, Pisolithus
aureosericeus sp. nov.
KUFF002 (2), KU351836, P
KUFF001, KU351837, H
100
KUFF003 (1), KU351838, P
KUFF003 (2), KU351839, P
51
KUFF003 (3), KU351840, P
100
2
Clade 11, P. aurantioscabrosus
Clade 13, P. indicus
100
69
100
2
Clade 1, Pisolithus sp.
5
Clade 12, P. abditus
(Clade 10, AF374624, Pisolithus sp.)
95
51
65
7
Clade 9, P. microcarpus
2
Clade 8 (Pisolithus sp.)
89
AF004732 (under Pisolithus sp.)
98
<50
22
Clade 7, P. albus complex
(Clade 10, AF374646, Pisolithus sp.)
(Clade 9, AF440867, P. microcarpus)
98
99
6
Clade 2, P. marmoratus
7
Clade 3, P. calongei
73
91
53
9
Clade 6, P. tinctorius
12
Clade 5, P. orientalis
12
Clade 4, P. arhizus
54
74
6
Clade 14, P. capsulifer
FM213344S, Scleroderma citrinum
GU373495, Suillus luteus
2.0
One of the 100 equally most parsimony trees obtained after
a heuristic search of the ITS sequence alignment (PAUP
v. 4.0b10). Following Phosri et al. (2012) and Martín et al.
(2013), sequences of Suillus luteus and Scleroderma citrinum
were included as outgroup. Pisolithus sequences were distributed in 15 main clades, clade number after Martin et al. (2002);
percentage of bootstrap values (> 50 %) are indicated on the
branches. The P. aureosericeus clade is marked with a grey
square (H: Holotype; P: Paratypes); the accession number
from EMBL/GenBank or UNITE databases are indicated to
the rest of terminals.
María P. Martín, Departamento de Micología, Real Jardín Botánico-CSIC, Plaza de Murillo 2, 28014 Madrid, Spain; e-mail: maripaz@rjb.csic.es
Tharnrat Kaewgrajang, Department of Forest Biology, Faculty of Forestry, Kasetsart University, 50 Ngamwongwan Rd, Latyao,
Chatuchak, Bangkok 10900, Thailand; e-mail: tarn_67@hotmail.com
Cherdchai Phosri, Faculty of Science, Nakhon Phanom University, 214, Moo 12, Nittayo Road, Nong Yart Sub-district, Muang District,
Nakhon Phanom, 48000, Thailand; e-mail: cherd_phosri@yahoo.co.uk
Roy Watling, Caledonian Mycological Enterprises, Vrelah, 26 Blinkbonny Avenue, Edinburgh, EH4 3HU,
Scotland, UK; e-mail: caledonianmyc@blueyonder.co.uk
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
424
Persoonia – Volume 36, 2016
Coprinus littoralis
Fungal Planet description sheets
425
Fungal Planet 453 – 4 July 2016
Coprinus littoralis G. Moreno, Carlavilla, Heykoop, Manjón, A. Sánchez, sp. nov.
Etymology. Name reflects the habitat, littoral dunes, from which this
fungus was collected.
Classification — Agaricaceae, Agaricales, Agaricomycetes.
Cap up to 45 × 25 mm (measured on dried herbarium specimens), ovoid to broadly ellipsoid, becoming revolute at margin
when mature and strongly deliquescent, first whitish, later with
pinkish tinges, veil thick, ochraceous, persistent at centre,
star-shaped, not deliquescent, recalling that of Coprinus
vosoustii. Gills crowded, first white, then pinkish, later black,
strongly deliquescent; gill-edge could not be observed due to
deliquescence. Stem 55–65 × 3–6 mm, whitish, with whitish
ephemeral ring; base up to 13 mm wide and bulbous to napiform, strongly rooting, 20–35 mm in length; hollow, with central
strand. Spores 13–20 × 8–12 µm av. 15.5–17.5 × 9.5–10.6
µm (3 collections), Qav. = 1.60–1.72, ellipsoid, smooth, sometimes slightly broadened base, dark black, germ pore central to
slightly eccentric toward the abaxial spore side, up to 2.5–3 µm
diam. Basidia and pseudoparaphyses could not be observed
due to deliquescence. Pleurocystidia not observed. Cheilo
cystidia probably present in young specimens but the material
studied was always very mature, with the gill-edge completely
deliquesced. Clampconnections absent, only pseudoclamps
present. Elements of veil 45–200 × 5–30 µm, consisting of
cylindrical septate hyphae, rarely branched, densely packed,
very variable in size and shape.
Habit, Habitat & Distribution — Growing solitary on sand in
littoral dunes with psammophilous vegetation. Very rare in the
studied area.
Typus. sPain, Huelva, Playa Coto de Doñana, National Park of Doñana,
psammophilous in dunes, 5 Apr. 2013, A. Sánchez (holotype AH 45819,
ITS sequence GenBank KU686920, LSU sequence GenBank KU686903,
MycoBank MB815823).
Additional specimens examined. Coprinus littoralis: sPain, Huelva,
Playa de Doñana, National Park of Doñana, psammophilous in dunes, 6
Apr. 2013, A. Sánchez, paratype AH 45860 (ITS, LSU sequences GenBank,
KU686921, KU686904), idem, 7 Apr. 2013, AH 45859 (ITS, LSU sequences
GenBank, KU686922, KU686905). Coprinus comatus: sPain, Alcalá de
Henares, Campus universitario, Facultad de Biología, in a garden, 15 Nov.
2008, J. Rejos & G. Moreno, AH 44095 (ITS, LSU sequences GenBank,
KU686915, KU686898); Madrid, Valdemorillo, in open area in a forest of
Quercus ilex subsp. ballota, 10 Apr. 2010, M. Hinojosa & J.C. Campos, AH
45823 (ITS, LSU sequences GenBank, KU686913, KU686896); Madrid,
Canillejas, in parking of the Capricho Park, 23 Mar. 2014, J.L. Domingo, AH
44089 (ITS, LSU sequences GenBank, KU686914, KU686897); Madrid,
Las Matas, on side of a path, 8 May 2014, I. Morales, AH 45796 (ITS, LSU
sequences GenBank, KU686916, KU686899); Guadalajara, in a garden,
6 Dec. 2014, J.R. Carlavilla, AH 45795 (ITS, LSU sequences GenBank,
KU686911, KU686894); Alcalá de Henares, Campus universitario, Residencia Crusa, in a garden, 28 Oct. 2015, P. Rosario, AH 45832 (ITS, LSU
sequences GenBank, KU686912, KU686895); idem, 4 Nov. 2015, AH
45831 (ITS, LSU sequences GenBank, KU686918, KU686901); Alcalá de
Henares, Campus universitario, Escuela Politécnica, in a garden, 6 Nov.
2015, A. LópezVillalba, J.R. Carlavilla & G. Moreno, AH 45830 (ITS, LSU
sequences GenBank, KU686917, KU686900). Coprinus pinetorum: sPain,
Madrid, Rivas Vaciamadrid, in humus of Pinus halepensis, 18 Nov. 2011,
M. Martín, L. RubioCasas, L. RubioRoldán & G. Moreno, holotype AH
44094 (ITS, LSU sequences GenBank, KU686924, KU686907); Madrid,
Rivas Vaciamadrid, in humus of Pinus halepensis, 18 Nov. 2011, M. Martín,
L. RubioCasas, L. RubioRoldán & G. Moreno, AH 45797 (ITS, LSU sequences GenBank, KU686925, KU686908); idem, 22 Nov. 2014, M. Martín,
AH 45798 (ITS, LSU sequences GenBank, KU686926, KU686909); Almería, Sierra de los Filabres, in humus of Pinus halepensis, 30 Nov. 2002,
G. Moreno & R. Galán, AH 45815 (ITS, LSU sequences GenBank, KU686927,
KU686910). Coprinus vosoustii: sPain, Madrid, Ciudad Universitaria, Facultad de Farmacia and Medicina, in a garden, 13 May 1976, K. Tabba, AH 1284
(ITS, LSU sequences GenBank, KU686919, KU686902); idem, 8 May 1977,
G. Moreno, AH 556 (ITS, LSU sequences GenBank, KU686923, KU686906).
Notes — Coprinus littoralis is characterised by its medium
size sporocarp (as compared with Coprinus comatus), its large
spores (13–20 × 8–12 µm) with slightly eccentric germ pore
and by growing in littoral dunes.
In our ITS phylogeny (MycoBank supplementary data) Coprinus
littoralis is significantly related to C. comatus, C. sterquilinus,
C. vosoustii and C. pinetorum. They all belong to subsect. Co
prinus s. Uljé (the C. comatus group). Coprinus comatus differs
from C. littoralis by its more robust habit, smaller spores (9–12.5
× 7–9 µm) and by fruiting on strongly nitrified sites (gardens,
roadsides and paths or on lawns). Coprinus sterquilinus differs
from C. littoralis by its larger spores (17–26 × 10–15 µm) and
the habitat on dung. Coprinus vosoustii, considered by Moreno
& Heykoop (1998) as a synonym of C. calyptratus, resembles
C. littoralis because of the thick and persistent star-shaped
ochraceous veil on the cap as well as by the large spores.
Nevertheless, Coprinus littoralis differs from C. vosoustii by the
strict psammophilous habitat and the absence of a napiform
rooting stipe. Coprinus pinetorum differs from C. littoralis by its
fibrillose flocculose veil, smaller spores (8–11 × 5.5–8 µm) and
by fruiting among needles of Pinus halepensis.
Macroscopically, Coprinus spadiceisporus, a very rare species
described from the State of Washington by Van de Bogart
(1976), is also a C. comatus-like fungus. Nevertheless, it differs
from C. littoralis by fruiting on dung and by its veil with small
somewhat appressed scales (Van de Bogart 1976). Uljé et al.
(1998) revised the type of C. spadiceisporus and synonymised
it with C. roseistipitatus, which also was described fruiting on
dung of rabbit and deer. Coprinus spadiceisporus has been
collected in Spain (Lleida) by Tabarés & Rocabruna (2002)
fruiting on dung of rabbit. Another C. comatus-like fungus which
resembles Coprinus littoralis is C. levisticolens. However, Co
prinus levisticolens differs from C. pinetorum by its scaly cap,
smaller spores (11–14.5 × 7–8 µm) and by fruiting on sandy soil
under Populus alba and Crataegus spp. (Ludwig & Roux 1995).
Colour illustrations. Spain, Playa Coto de Doñana, National Park of
Doñana, littoral dunes with psammophilous vegetation, where the holotype
was collected; basidiomata, cylindrical septate hyphae of veil, spores under
LM, smooth spores with eccentric germ pore under SEM (from the holotype).
Scale bars = 1 cm (basidiomata), 50 µm (veil), 10 µm (spores under LM), 5 µm
(spores under SEM).
Gabriel Moreno, Juan Ramón Carlavilla, Michel Heykoop, José Luis Manjón & A. Sánchez, Departamento de Ciencias de la Vida (Área de Botánica),
Universidad de Alcalá, E-28805 Alcalá de Henares, Madrid, Spain;
e-mail: gabriel.moreno@uah.es, juan.carlavilla@gmail.com, michel.heykoop@uah.es, josel.manjon@uah.es & antoniosanchez48@hotmail.com
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
426
Persoonia – Volume 36, 2016
Coprinus pinetorum
427
Fungal Planet description sheets
Fungal Planet 454 – 4 July 2016
Coprinus pinetorum G. Moreno, Carlavilla, Heykoop & Manjón, sp. nov.
Etymology. Name reflects the habitat, humus of Pinus halepensis, from
which this fungus was collected.
Classification — Agaricaceae, Agaricales, Agaricomycetes.
Cap 35–45 × 25–35 mm when still closed, ellipsoid to subcylindrical, becoming revolute at margin when mature, first whitish,
later with pinkish tinges, veil first fibrillose to flocculose, then
breaking up into small and fragile whitish upturned scales,
except at centre, that stays smooth and becomes creamy to
strawish creamy. Gills crowded, first white, and then pinkish to
black, the gill-edge whitish, probably due to the presence of
numerous cheilocystidia, strongly deliquescent when mature.
Stem 60–120 × 10–17 mm, whitish, with movable fragile ring on
lower part, upperside strawish whitish, underside greyish flesh
coloured, with up to 22 mm wide bulbous to subbulbous base,
hollow, with central strand. Spores 8–11 × (5–)5.5–8 µm av.
8.1–10.6 × 5.9–6.9 µm (4 collections), Qav. = 1.38–1.62, smooth,
ellipsoid, sometimes slightly broadened base, dark black, germ
pore central, up to 1–1.5 µm diam. Basidia 14–35 × 10–13 µm
(excl. sterigmata), sterigmata up to 4 µm in length, 4-spored,
hyaline, sometimes with brownish vacuolar pigment toward
the base, surrounded by 6–8 pseudoparaphyses. Cheilocys
tidia probably present in young specimens but the material
studied was always very mature, with the gill-edge completely
deliquesced. Pleurocystidia not observed. Clampconnections
absent, only pseudoclamps present. Elements of veil 35–220
× 9–35 µm, consisting of cylindrical branched hyphae, septate,
densely packed and very variable in size and shape.
Habit, Habitat & Distribution — Growing gregarious on basic
soil under Pinus halepensis. Rare in the studied area.
Typus. sPain, Madrid, Rivas Vaciamadrid, in humus of Pinus halepensis,
18 Nov. 2011, M. Martín, L. RubioCasas, L. RubioRoldán & G. Moreno
(holotype AH 44094, ITS sequence GenBank KU686924, LSU sequence
GenBank KU686907, MycoBank MB815824).
Additional specimens examined. Coprinus pinetorum: sPain, Madrid,
Rivas Vaciamadrid, in humus of Pinus halepensis, 18 Nov. 2011, M. Martín,
L. RubioCasas, L. RubioRoldán & G. Moreno, paratype AH 45797 (ITS, LSU
sequences GenBank, KU686925, KU686908); idem, 22 Nov. 2014, M. Martín,
paratype AH 45798 (ITS, LSU sequences GenBank, KU686926, KU686909);
Almería, Sierra de los Filabres, in humus of Pinus halepensis, 30 Nov. 2002,
G. Moreno & R. Galán, paratype AH 45815 (ITS, LSU sequences GenBank,
KU686927, KU686910). Coprinus comatus: sPain, Alcalá de Henares,
Campus universitario, Facultad de Biología, in a garden, 15 Nov. 2008, J.
Rejos & G. Moreno, AH 44095 (ITS, LSU sequences GenBank, KU686915,
KU686898); Madrid, Valdemorillo, in open area in a forest of Quercus ilex
subsp. ballota, 10 Apr. 2010, M. Hinojosa & J.C. Campos, AH 45823 (ITS,
LSU sequences GenBank, KU686913, KU686896); Madrid, Canillejas, in
parking of the Capricho Park, 23 Mar. 2014, J.L. Domingo, AH 44089 (ITS,
LSU sequences GenBank, KU686914, KU686897); Madrid, Las Matas, on
side of a path, 8 May 2014, I. Morales, AH 45796 (ITS, LSU sequences GenBank, KU686916, KU686899); Guadalajara, in a garden, 6 Dec. 2014, J.R.
Carlavilla, AH 45795 (ITS, LSU sequences GenBank, KU686911, KU686894);
Alcalá de Henares, Campus universitario, Residencia Crusa, in a garden, 28
Oct. 2015, P. Rosario, AH 45832 (ITS, LSU sequences GenBank, KU686912,
KU686895); idem, 4 Nov. 2015, AH 45831 (ITS, LSU sequences GenBank,
KU686918, KU686901); Alcalá de Henares, Campus universitario, Escuela
Politécnica, in a garden, 6 Nov. 2015, A. LópezVillalba, J.R. Carlavilla &
G. Moreno, AH 45830 (ITS, LSU sequences GenBank, KU686917,
KU686900). Coprinus littoralis: sPain, Huelva, Playa Coto de Doñana,
National Park of Doñana, psammophilous in dunes, 5 Apr. 2013, A. Sánchez,
holotype AH 45819 (ITS, LSU sequences GenBank, KU686920, KU686903);
Huelva, Playa de Doñana, National Park of Doñana, psammophilous in
dunes, 6 Apr. 2013, A. Sánchez, AH 45860 (ITS, LSU sequences GenBank,
KU686921, KU686904), idem, 7 Apr. 2013, AH 45859 (ITS, LSU sequences
GenBank, KU686922, KU686905). Coprinus vosoustii: sPain, Madrid, Ciudad Universitaria, Facultad de Farmacia y Medicina, in a garden, 13 May 1976,
K. Tabba, AH 1284 (ITS, LSU sequences GenBank, KU686919, KU686902);
idem, 8 May 1977, G. Moreno, AH 556 (ITS, LSU sequences GenBank,
KU686923, KU686906).
Notes — Coprinus pinetorum is characterised by its small size
(as compared with C. comatus), its spores with slightly broadened base (8–11 × 5.5–8 µm) and by fruiting among needles
of Pinus halepensis.
In our ITS phylogeny (MycoBank supplementary data) Coprinus
pinetorum is significantly related to C. comatus, C. sterquilinus,
C. vosoustii and C. littoralis. They all belong to subsect. Copri
nus s. Uljé (the C. comatus group). Coprinus comatus differs
from C. pinetorum by its more robust habit and by fruiting on
strongly nitrified sites (gardens, roadsides and paths or on
lawns). Coprinus sterquilinus differs from C. pinetorum by its
solitary basidiocarps, very large spores (17–26 × 10–15 µm)
and the habitat on dung. Coprinus vosoustii differs from C. pine
torum by its ovoid cap, thick ochraceous and persistent veil
forming a star-shaped layer at centre, and much larger spores
(17–20 × 10–12 µm). Coprinus littoralis differs from C. pine
torum by its larger basidiocarps, larger spores (13–20 × 8–12
µm) with slightly eccentric germ pore and the psammophilous
habitat in littoral dunes.
Macroscopically, C. spadiceisporus, a very rare species described from the State of Washington by Van de Bogart (1976),
is also a C. comatus-like fungus. Nevertheless, it differs from
C. pinetorum by fruiting on dung and by its spores with eccentric germ pore (Van de Bogart 1976). Another C. comatus-like
fungus which resembles Coprinus pinetorum is C. levisticolens.
However, C. levisticolens differs from C. pinetorum by its scaly
cap, larger spores (11–14.5 × 7–8 µm) with eccentric germ pore
and by fruiting on sandy soil under Populus alba and Crataegus
spp. (Ludwig & Roux 1995).
Colour illustrations. Spain, Rivas Vaciamadrid, humus of Pinus halepen
sis, where the holotype was collected; basidiomata and fruit body section,
cylindrical hyphae of veil, 4-spored basidia, spores under LM, smooth spores
with central germ pore under SEM (from the holotype). Scale bars = 1 cm
(basidiomata), 10 µm (veil, basidia, spores under LM), 2 µm (spores under
SEM).
Gabriel Moreno, Juan Ramón Carlavilla, Michel Heykoop & José Luis Manjón, Departamento de Ciencias de la Vida (Área de Botánica),
Universidad de Alcalá, E-28805 Alcalá de Henares, Madrid, Spain;
e-mail: gabriel.moreno@uah.es, juan.carlavilla@gmail.com, michel.heykoop@uah.es & josel.manjon@uah.es
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
428
Persoonia – Volume 36, 2016
Priceomyces vitoshaensis
a
b
c
429
Fungal Planet description sheets
Fungal Planet 455 – 4 July 2016
Priceomyces vitoshaensis Gouliamova, Dimitrov, M.T. Sm., M.M. Stoilova-Disheva &
M. Groenew., sp. nov.
Etymology. The specific epithet ‘vitoshaensis’ was derived from the locality
Vitosha Nature Park where insect hosts of the ex-type strain were collected.
Classification — Debaryomycetaceae, Saccharomycetales,
Saccharomycetes.
After 7 d growth at 25 °C on 5 % glucose broth, cells are globose, ovoid, oblong, 2–5 × 3–7 μm, occurring singly, in pairs,
in small clusters or in small chains, and proliferating by multilateral budding. Dalmau plate culture after 10 d on morphology
(Wickerham 1951) and potato-dextrose agar (PDA) at 20–25 °C
did not show pseudohyphae. After 3 d of incubation on yeast
extract, malt extract, pepton, glucose agar (YM), the single
strains formed asci after conjugation of independent cells. The
asci were lytic, releasing 1–2 round and smooth ascospores.
For physiological characteristics see MycoBank MB802453.
Typus. bulGaria, Sofia, Nature Park Vitosha, in birch forest, above village
Bistritsa, N42°35'10" E23°21'36", from Pterostichus melas (Carabidae) 19
July 2009, D. Gouliamova (holotype metabolically inactive strain CBS 12457;
ITS sequence GenBank HM627157.2, LSU sequence GenBank HM627053,
MycoBank MB802453); Additional strain 3R = CBS 1243 was isolated from
the same beetle species collected in Nature Park ‘Zlatni Pyasatsi’, N43°17'0"
E28°2'0", on oak meadow, 24 Apr. 2009, ITS, LSU sequences GenBank
KC810955, KC810946.
Notes — The most similar sequence in GenBank is C. north
wykensis (98 % identity in both LSU and ITS sequences). Phylogenetic analyses, using an alignment of concatenated LSU and
ITS sequences of known species present in the Priceomyces
clade and the new yeast strains, placed the latter with C. north
wykensis in a separate subclade (100 % support). Pairwise
comparison of sequences from multiple alignment data showed
that the new strains have 97 % identity with C. northwykensis
(1 121 identical nt., 14 subst. in ITS and 11 subst., 1 gap in LSU),
93 % with C. fermenticarens (1 080 identical nt., 44 subst.,
10 gaps in ITS and 23 subst., 3 gaps in LSU) and 91 % identity
with P. melissophilus (1 054 nt., 47 subst., 10 gaps in ITS and
24 subst., 2 gaps in LSU). Nine physiological characteristics
distinguish the new strains from C. northwykensis. They can assimilate cellobiose, salicin, ribitol, succinate, methyl α-glucoside
and nitrite. They cannot assimilate ethanol and they do not
grow in 0.01 % cycloheximide and at 35 °C. Thus, we assign
these strains to the newly proposed species, Priceomyces
vitoshaensis.
New combinations in the genus Priceomyces.
Based on the results of phylogenetic analysis of combined
LSU and ITS rDNA we propose new combinations in the genus
Priceomyces for the following species that previously belonged
to the genus Candida.
Priceomyces fermenticarens (Van der Walt & Baker) Gouliamova, Dimitrov, M.T. Sm., M.M. Stoilova-Disheva &
M. Groenew., comb. nov. — MycoBank MB310255
Basionym. Candida fermenticarens, Van der Walt & Baker, Bothalia 12: 561.
1978.
Priceomyces northwykensis (Ravella et al.) Gouliamova,
Dimitrov, M.T. Sm., M.M. Stoilova-Disheva & M. Groenew.,
comb. nov. — MycoBank MB560189
Basionym. Candida northwykensis, Ravella et al., Curr. Microbiol. 63: 115.
2011.
0.02
72 Priceomyces vitoshaensis 3 HM627157.2/HM627053
100
Priceomyces vitoshaensis 3R KC810955/KC810946
Priceomyces northwykensis NCYC-3525T FM179646/FM179640
82
100
Priceomyces fermenticarens Y-17321T EU343829/AB013525
100
Priceomyces melissophilus Y-7585T AB054021/U45740
71 Priceomyces castillae Y-7501T DQ409168/U45769
100
100
Priceomyces medius Y-7122T AB054119/U45768
Priceomyces haplophilus Y-7860T DQ409169/U45770
Priceomyces carsonii YB-4275T AB054097/U45743
100
Millerozyma acaciae Y-7117T EU343866/U45767
Millerozyma farinosa Y-7553T FR668054/U45739
Colour illustrations. Bulgaria, Vitosha Mountain, large stone river Zlatnite
Mostove (Golden Bridges) (Photo: Alexandra Toneva); a. Pterostichus melas
(Carabidae, www.zin.ru /Animalia /Coleoptera); b. morphology of cells of
Priceomyces vitoshaensis 3T in 5 % glucose broth; c. arrows indicting the
conjugated asci with ascospores. Scale bars = 10 μm (cells), 5 μm (asci with
ascospores).
Phylogenetic analysis of the alignment of the ITS1+2 region,
and the LSU (D1/D2 domains) rRNA gene using a maximum
likelihood analysis (MEGA v. 6) for Priceomyces vitoshaensis
3 T and related species.
Dilnora E. Gouliamova & Margarita M. Stoilova-Disheva, The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences,
Acad. G. Bonchev 26, Sofia 1113, Bulgaria; e-mail: dilnorag@gmail.com & margid@microbio.bas.bg
Roumen A. Dimitrov, Sofia University “St. Kliment Ohridski”, 5 James Bourchier Blvd., Sofia 1164, Bulgaria; e-mail: dimitrov@phys.uni-sofia.bg
Maudy Th. Smith & Marizeth Groenewald, CBS-KNAW Fungal Biodiversity Centre, Box 85167, 3508 AD Utrecht, The Netherlands;
e-mail: m.smith@cbs.knaw.nl & m.groenewald@cbs.knaw.nl
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
430
Persoonia – Volume 36, 2016
Nothophoma macrospora
431
Fungal Planet description sheets
Fungal Planet 456 – 4 July 2016
Nothophoma macrospora Valenzuela-Lopez, Stchigel, Cano & Deanna A. Sutton,
sp. nov.
Etymology. G. μακρό-, large, and -σπορά, spore, referring to the big size
of the conidia.
Classification — Didymellaceae, Pleosporales, Dothideo
mycetes.
Hyphae pale to dark brown, 3–10 μm wide, thin- to thick-walled,
smooth to granulose due to the production of dark granules, septate, anastomosing. Conidiomata pycnidial dark brown, pyriform
to heart-shaped by the occasional production of 2–3(–4) necks,
rarely globose, 100–300 × 100–300 μm; peridium 3–5-layered,
15–25 μm thick, peridial cells globose to polygonal, pale to
dark brown, 5–10 μm diam, thick-walled; neck usually present,
paler than the peridial wall, cylindrical to conical, (50–)90–150
× (50–)80–110 μm, papillate, ornamented with a crown of short,
subhyaline, conical to digitiform projections around the ostiolum,
ostiolum of late opening; exuded conidial masses not observed;
conidiogenous cells enteroblastic, phialidic, globose to flaskshaped, hyaline, thin-walled, 5–10 μm diam; conidia (9–)10–15
× 2.5–3(–3.5) μm, hyaline, cylindrical to slightly clavate at one
or both ends, 0(- 2)-septate, narrowing slightly at the septa,
guttulate, sometimes producing a similar conidia on a lateral
bulge, then forming irregular chains. Chlamydospores absent, but some hyphae cells become darker, thicker and barrelshaped.
Culture characteristics — Colonies on OA reaching 30 mm
diam in 7 d at 25 °C, olive brown (M.4F3), flattened, granulose
due to the production of numerous pycnidia; reverse concolorous. Colonies on MEA attaining 37–41 mm in 7 d at 25 °C,
yellowish white (M.4A2) to light brown (M.6D8), flattened, compact, reverse concolorous. NaOH spot test: negative. Crystals
absent.
Typus. usa, Arizona, Phoenix, from respiratory secretion of a patient with
pneumonia, 1 Apr. 2009, D.A. Sutton (holotype CBS H-22377, cultures extype UTHSC DI09-853 = FMR 13767 = CBS 140674, ITS sequence GenBank
LN880536, LSU sequence GenBank LN880537, actA sequence GenBank
LN880538, tub2 sequence GenBank LN880539, MycoBank MB815051).
89
0.98
72
0.95
Nothophoma anigozanthi CBS 381.91
Nothophoma quercina CBS 633.92
Nothophoma infossa CBS 123395 T
86
100
1
Notes — This fungus was isolated from a human clinical
specimen. Morphologically, Nothophoma macrospora resembles the species previously classified into Phoma section Macro
spora (Boerema et al. 2004), i.e. Phoma andropogonivora,
P. boeremae, P. chenopodii, P. commelinicola, P. gossypiicola,
P. necator, P. rabiei, P. xanthina and P. zeaemaydis. These
species produce the largest conidia of the genus. Based on a
megablast search of NCBIs GenBank nucleotide database, the
closest hits using the LSU sequence are Peyronellaea combreti
(GenBank KJ869191; Identities = 887/889 (99 %), no gaps)
and Peyronellaea prosopidis (GenBank KF777232; Identities
= 887/889 (99 %), no gaps). Closest hits using ITS sequence
are Leptosphaerulina australis (GenBank KF293970; Identities
= 493/497 (99 %), gaps 1/497 (0 %)), Didymella glomerata
(GenBank AB369471; Identities = 493/497 (99 %), gaps 1/497
(0 %)) and Nothophoma quercina (GenBank AB369461; Identities = 493/497 (99 %), gaps 1/497 (0 %)). In a similar search in
the Q-Bank fungal nucleotide database (www.q-bank.eu), the
closest hit is Nothophoma anigozanthi CBS 381.91 (Identities
= 468/473 (99 %), gaps = 1/473 (0 %)). The closest hit using
the beta-tubulin (tub2) sequence is Nothophoma gossypiicola
(GenBank GU237611; Identities = 323/335 (99 %), no gaps),
as well was using the actin (actA) sequence against Q-Bank
(Nothophoma gossypiicola CBS 377.67; Identities = 214/224
(96 %), no gaps). Our phylogenetic tree, built by using the ITS,
LSU, tub2 and actA sequences, corroborated that our fungus
represents a new species of the genus Nothophoma, N. gos
sypiicola being the most phylogenetically and morphologically
related species. Nothophoma macrospora differs from N. gos
sypiicola by its lower growing rate on OA, the shape (pyriform
to hearth-shaped vs globose), the number of necks (up to 4
vs 0–1) and the ornamentation (papillate vs non-papillate) of
the pycnidia, and the presence of conidial septa (up to 2 vs
non-septate).
95
1
Nothophoma macrospora sp. nov. UTHSC DI09-853
Nothophoma gossypiicola CBS 377.67
Phoma bulgarica CBS 357.84 T
Phoma herbarum CBS 615.75
Phomatodes nebulosa CBS 117.93
Neoascochyta paspali CBS 560.81 T
Neoascochyta paspali CBS 561.81
0,02
Colour illustrations. USA, Arizona, Phoenix, McDowell mountain park
(image credit: Hector Lopez and Brenda, www.hmlopezphoto.com); colony
on OA after 7 d at 25 °C, conidiomata under stereomicroscope, pycnidia,
conidiogenous cells, conidia. Scale bars = 10 µm.
Maximum likelihood tree obtained from the combined DNA
sequences dataset from four loci of our isolate and sequences
retrieved from the GenBank and the Q-Bank databases (TreeBASE ID 18137). Above the nodes are presented the bootstrap
support values ≥ 70 %, and the Bayesian posterior probability
scores ≥ 0.95 are indicated below. Neoascochyta paspali (CBS
560.81 & CBS 561.81) was used as outgroup. Ex-type strains
of the different species are indicated with T. The new species
proposed in this study is indicated in bold. The alignment was
performed by MEGA v. 6.06 (Tamura et al. 2013), and the tree
building by MEGA v. 6.06 and by MrBayes v. 3.2.4 (Huelsenbeck
& Ronquist 2001).
Nicomedes Valenzuela-Lopez, Mycology Unit, Medical School and IISPV, Universitat Rovira i Virgili (URV), Sant Llorenç 21, 43201 Reus, Tarragona, Spain;
Microbiology Unit, Medical Technology Department, Faculty of Health Science, University of Antofagasta, Av. Universidad de Antofagasta s/n,
02800 Antofagasta, Chile; e-mail: nicomedes.vl@gmail.com
Alberto M. Stchigel & José F. Cano-Lira, Mycology Unit, Medical School and IISPV, Universitat Rovira i Virgili (URV), Sant Llorenç 21,
43201 Reus, Tarragona, Spain; e-mail: alberto.stchigel@urv.cat; jose.cano@urv.cat & josep.guarro@urv.cat
Deanna A. Sutton & Nathan P. Wiederhold, Fungus Testing Laboratory, Department of Pathology, University of Texas Health Science Center,
7703 Floyd Curl Dr., San Antonio, Texas 78229-3900, USA; e-mail: suttond@uthscsa.edu
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
432
Persoonia – Volume 36, 2016
Entoloma kruticianum
433
Fungal Planet description sheets
Fungal Planet 457 – 4 July 2016
Entoloma krutiсianum O.V. Morozova, M.Yu. Dyakov, E.S. Popov &
A.V. Alexandrova, sp. nov.
Etymology. The epithet refers to the type locality – Krutitsy Village in the
Tver Region of Russia.
Classification — Entolomataceae, Agaricales, Agaricomy
cetes.
Basidiomata small-sized, mycenoid. Pileus 5 ‒12 mm diam,
hemispherical to convex, not hygrophanous, not translucently
striate, with appendiculate margin, radially fibrillose to slightly
squamulose in the centre, deep violet (15E7– 8; Kornerup &
Wanscher 1978), darker in the centre (15F6–8). Lamellae moderately distant, adnate-emarginate or almost free, ventricose,
reddish lilac (14C3–4, 14D3 –4), becoming greyish pink, with
paler entire edge. Stipe 20–70 × 1–2 mm, cylindrical, longitudinally fibrillose-striate, deep blue to deep violet (19D8 –E8,
18D8 –E8), clearly different from the pileus, white tomentose
at base. Context concolorous with the surface. Smell indistinct,
taste not reported. Spores (7.5–)8.5(–10.5) × (5.5–)6(–7) μm,
Q = (1.3–)1.5(–1.8), heterodiametrical, with 5–7 angles in sideview. Basidia 22–25 × 8–12.5 μm, 4-spored, narrowly clavate
to clavate, clamped. Lamellae edge fertile or heterogeneous.
Cheilocystidia 24.5–37.5 × 4.5–9.5 μm, cylindrical, lageniform
or irregularly shaped, intermixed with basidia, in some basidiomata rare or absent. Pileipellis a plagiotrichoderm to trichoderm
in the centre, of cylindrical to slightly inflated hyphae 10–20 μm
wide with swollen terminal elements and bluish-violaceous
intracellular pigment. Clamp-connections present.
Habit, Habitat & Distribution — In a small group among
Sphagnum in Picea abies forest. Known from European Russia.
spores and more conical pileus, E. chytrophilum possesses
white lamellae, nodulose spores and more applanate pileus.
Both varieties of E. callichroum are characterized by the similar
coloration of the basidiomata, but their spores are larger and
cheilocystidia (if present) are broadly clavate, never lageniform.
One more species with coloured lamellae – E. euchroum – usually is more robust and possesses larger spores with rather
blunt angles.
Typus. russia, Tver Region, Staritsa District, vicinities of the Krutitsy Village, N56°18'35.2" E34°52'07.7", 13 Sept. 2015, M. Dyakov, O. Morozova,
E. Popov & A. Alexandrova (holotype LE 311767, ITS sequence GenBank
KU666558, LSU sequence GenBank KU710222, MycoBank MB815745).
Notes — Entoloma krutiсianum represents a species of the
subgenus Leptonia due to the presence of clamp-connections,
absence of brilliant granules and plagiotrichoderm to trichoderm
pileipellis. It resembles E. lepidissimum by its small-sized mycenoid basidiomata with deep blue and violet colours, including
coloured lamellae. Microscopically, the scattered cheilocystidia
also make them similar. However, E. krutiсianum can be recognized by the colour of the pileus, which is clearly different from
the stipe, the presence of the reddish lilac tint in the lamellae,
as well as smaller spores with pronounced angles and attenuate hilum. The ITS1-5.8S-ITS2 region of the newly described
species has been compared with those of the other Leptonia
species (mostly derived from the type material, data from Morozova et al. 2014). Molecular data support their differences
(p-distance from the closest species E. lepidissimum – 8 %).
The similar dark blue species distinguish (except for the colour
of the pileus): E. coelestinum – by the white lamellae, smaller
Colour illustrations. Russia, Tver’ Region, Staritsa District, vicinities of
the Krutitsy Village, type locality; pileipellis, spores, cheilocystidia, basidiomata (all from holotype). Scale bars = 1 cm (basidiomata), 10 µm (spores,
cheilocystidia and pileipellis).
Phylogenetic tree derived from Bayesian analysis, based on
nrITS1-5.8S-ITS2 data. Analysis was performed under GTR
model, for 3 M generations, using MrBayes v. 3.2.1 (Ronquist
et al. 2012). The ML analysis was run in the RAxML server
(http://phylobench.vital-it.ch/raxml-bb/index.php (Stamatakis
et al. 2008)). Posterior probability (PP > 0.95) values from
the Bayesian analysis followed by bootstrap values from the
Maximum Likelihood (BS > 70 %) analysis are added to the
left of a node (PP/BS).
Olga V. Morozova & Eugene S. Popov, Laboratory of Systematics and Geography of Fungi,
Komarov Botanical Institute of the Russian Academy of Sciences, 197376, 2 Prof. Popov Str., Saint Petersburg, Russia;
e-mail: OMorozova@binran.ru & EPopov@binran.ru
Alina V. Alexandrova & Maxim Yu. Dyakov, Lomonosov Moscow State University (MSU), Faculty of Biology, 119234, 1,
12 Leninskie Gory Str., Moscow, Russia;
e-mail: alina-alex2011@yandex.ru & max_fungi@mail.ru
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
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Persoonia – Volume 36, 2016
Xerocomellus poederi
Fungal Planet description sheets
435
Fungal Planet 458 – 4 July 2016
Xerocomellus poederi G. Moreno, Heykoop, Esteve-Rav., P. Alvarado & Traba, sp. nov.
Etymology. Named after Reinhold Pöder, Austrian mycologist and specialist in Boletales, who passed away in August 2015.
Classification — Boletaceae, Boletales, Agaricomycetes.
Cap 1.2–5.5 cm broad, convex becoming applanate convex,
sometimes depressed at centre, pale brown (Mu 7.5YR 6/3,
6/4), brown pinkish (Mu 5YR 6/3, 6/4) to dark brown when
mature (Mu 5YR 3/1, 3/2, 3/3), becoming darker in herbarium
specimen; surface dry, smooth, the epicutis cracking in age
with reddish tinges in the cracks on the upper part (Mu 10R
4/6, 4/8); context in cap whitish, reddish under the epicutis,
staining slightly bluish when bruised or cut. Margin irregular,
concolorous to slightly paler, not hygrophanous nor striate.
Tubes up to 0.8 mm in length, depressed around the stipe, ventricose, narrower towards the margin, whitish to pale yellowish
when young (Mu 2.5Y 8/3, 8/4) then yellowish (Mu 2.5Y 7/4,
7/6), turning slightly bluish when bruised or cut. Stem 2.5–6 ×
0.3–0.6 cm, cylindrical, solid, tapered downward, often curved,
fragile, dark reddish (Mu 10R 3/4, 3/6), yellowish at the apex
(Mu 2.5Y 8/4, 8/6), surface slightly granulose; context in stem
whitish at the apex, dark red in the lower half or lower two thirds,
turning slightly bluish when bruised or cut. Odour and taste not
distinctive. Sporeprint olive brown (Mu 2.5Y 5/3, 5/4). Spores
(10.5–)11–16(–17) × 4–5.5(–6) µm, av. 11.9–14.6 × 4.4–5
µm (8 collections), Qav. = 2.56 – 3.17, fusiform, smooth with
obtuse apex, with a distinct suprahilar depression, not amyloid
nor dextrinoid, containing 1–3 lipidic globose drops or one ellipsoid drop filling almost the entire spore volume; under the
SEM spores lack any ornamentation. Basidia 4-spored, 33–45
× 9–13 µm, sterigmata up to 5 µm in height, clavate, hyaline.
Pleurocystidia numerous, fusiform, with obtuse apex, 33–55 ×
8–12 µm, hyaline, sometimes with yellowish content. Cheilo
cystidia similar to pleurocystidia. Caulohymenium throughout
the stem consisting of basidia, basidioles and cystidia similar
to those present in the hymenium. Pileipellis a trichodermium
consisting of septate hyphae, the cylindrical cells of the hyphae
with thick walls, terminal cells very variable in shape, cylindrical
(narrower than lower cells) to lageniform, 30–40 × 9–11 µm,
with abundant yellowish pigment forming patches or strips.
Clampconnections not seen.
Habit, Habitat & Distribution — Growing solitary to gregarious on acid soil under Quercus robur. Very abundant in the
studied area.
Typus. sPain, Lugo, Parque Río Rato, Concello de Lugo, in humus of
Quercus robur, 1 Nov. 2013, G. Moreno & J.M. Traba (holotype AH 44050,
ITS sequence GenBank KU355475, LSU sequence GenBank KU355488,
MycoBank MB815475).
Additional specimens examined. Xerocomellus poederi: sPain, Lugo,
Parque Río Rato, Concello de Lugo, in humus of Quercus robur, 1 Nov.
2013, G. Moreno & J.M. Traba, paratype AH 44051 (ITS, LSU sequences
GenBank, KU355476, KU355489); idem, paratype AH 44052 (ITS, LSU
Colour illustrations. Spain, Parque Río Rato, Quercus robur forest, where
the holotype was collected; basidiomata and fruit body section, trichodermium
showing its variability, 4-spored basidium, hymenial cystidia, basidiospores,
smooth spores under SEM (holotype AH 44050). Scale bars = 1 cm (basidiomata), 10 µm (pileipellis, basidium, pleurocystidia, spores under LM), 2 µm
(spores under SEM).
sequences GenBank, KU355477, KU355490); idem, paratype AH 44053;
idem, paratype AH 45804 (ITS, LSU sequences GenBank, KU355478,
KU355491); Lugo, Concello de O Corgo, Finca O Fia, in humus of Quercus
robur, 2 Nov. 2013, G. Moreno, J.M. Traba & J.M. CastroMarcote, paratype
AH 45855; A Coruña, Vimianzo, in humus of Quercus robur, Corylus avellana
and Laurus nobilis, 29 Aug. 2015, J.M. CastroMarcote, paratype AH 45803
(ITS, LSU sequences GenBank, KU355480, KU355491); Orense, Leiro, in
humus of Quercus robur, 23 Nov. 2013, J.M. CastroMarcote, paratype AH
45805 (ITS, LSU sequences GenBank, KU355479, KU355492). Xerocomellus chrysenteron: sPain, Madrid, La Barranca, Navacerrada, in humus of
Pinus sylvestris, 29 Oct. 2013, V. Córdoba, AH 44023 (ITS, LSU sequences
GenBank, KU355474, KU355487); Segovia, Ermita de Hontanares, Riaza,
in humus of Quercus pyrenaica, 20 June 2010, D. Saavedra, Y. Fernández
& L. RubioCasas, AH38968 (ITS, LSU sequences GenBank, KU355473,
KU355486). Xerocomellus porosporus: sPain, Segovia, Ermita de Hontanares, Riaza, in humus of Quercus pyrenaica, 12 June 2010, L. RubioRoldán
& L. RubioCasas, AH 38964 (ITS, LSU sequences GenBank, KU355481,
KU355493). Xerocomellus ripariellus: sPain, Madrid, Velilla de San Antonio, in humus of Populus alba, 3 July 2010, M. Martín, AH38971 (ITS, LSU
sequences GenBank, KU355482, KU355494). Xerocomus subtomentosus:
sPain, Ávila, El Tiemblo, in humus of Castanea sativa and Quercus pyrenaica,
27 June 2010, J.A. Rodea, AH38974 (ITS sequence GenBank, KU355483);
Toledo, Real de San Vicente, in humus of Quercus ilex ssp. ballota, 23 Nov.
2013, R. Losada, AH 44076 (ITS, LSU sequences GenBank, KU355484,
KU355495); Segovia, Fresno de Cantespino, in humus of Quercus pyrenaica
and Q. ilex ssp. ballota, 6 Sept. 2013, J.M. Barrasa, AH45790 (ITS, LSU sequences GenBank, KU355485, KU355496).
Notes — Xerocomellus poederi is morphologically characterised by its small size, the reddish cylindrical fusiform long
stem in relation to the cap diameter, the dark reddish context in
the lower part of the stipe and by fruiting isolated to gregarious
on acid soil under Quercus robur.
In our ITS phylogeny (MycoBank supplementary data) Xero
comellus poederi is closely related to X. chrysenteron, X. poro
sporus and X. sarnarii, and to a lesser degree to X. dryophilus,
all of them belonging to the difficult X. chrysenteron complex
(Peintner et al. 2003). The whole genus is strikingly different
from Xerocomus and other taxa. Xerocomellus chrysenteron
differs from X. poederi because of its more robust habit and
its differently coloured stem context, with greenish to yellowish
green tinges in the base, never dark reddish, and also because
of its association with conifers or Fagus in Southern Europe.
Xerocomellus porosporus differs from X. poederi because of
the dark brownish colours lacking dark reddish tinges in the
context at the base of the stem, as well as its apically truncate
spores with a distinct germ pore. Xerocomellus dryophilus, a
species described by Thiers (1975) from California, resembles
X. poederi because of the dark reddish context at the base of
the stem, a feature which led many authors to confuse both taxa
(Simonini 1994, Pérez de Gregorio 1995, Ladurner & Simonini
2003); however, it differs from X. poederi because of its larger
habit, the short stem in relation with the cap diameter, and a
strict association to Quercus agrifolia (coast Live Oak) (Bessette
et al. 2000, Desjardin et al. 2015). Xerocomellus sarnarii, a
species described recently by Ariyawansa et al. (2015) from
Italy, is macroscopically similar to X. poederi; however, it differs
from X. poederi because of its spores which have a small truncature at the apex and a strict association with Mediterranean
sclerophilous forests of Quercus ilex and Q. suber.
For the description of the colours the Munsell soil colour charts
were used (Munsell 1994).
Gabriel Moreno, Michel Heykoop, Fernando Esteve-Raventós, Departamento de Ciencias de la Vida (Área de Botánica), Universidad de Alcalá, E-28805
Alcalá de Henares, Madrid, Spain; e-mail: gabriel.moreno@uah.es, michel.heykoop@uah.es, fernando.esteve@uah.es
Pablo Alvarado, ALVALAB, La Rochela n° 47, E-39012, Santander, Spain; pablo.alvarado@gmail.com
José María Traba, Plaza de España 1, E 15001 A Coruña, Spain; chemitraba@gmail.com
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
436
Persoonia – Volume 36, 2016
Helminthosporiella stilbacea
437
Fungal Planet description sheets
Fungal Planet 459 – 4 July 2016
Helminthosporiella Hern.-Restr., G.A. Sarria & Crous, gen. nov.
Etymology. Similar to the genus Helminthosporium.
Classification — Massarinaceae, Pleosporales, Dothideo
mycetes.
Mycelium superficial and immersed, hyphae hyaline to pale
brown, smooth, branched, septate. Conidiophores erect, brown
to red-brown, synnematous, septate, compacted. Conidiog
enous cells polytretic, sympodial, integrated, determinate, ter-
minal, cylindrical. Conidia catenate in easily disarticulating
chains, obclavate, subcylindrical, occasionally bifurcate, brown,
distoseptate, hilum darkened, thickened and refractive. Sexual
morph unknown.
Type species. Helminthosporiella stilbacea (Moreau) Hern.-Restr., G.A.
Sarria & Crous.
MycoBank MB816988.
Helminthosporiella stilbacea (Moreau) Hern.-Restr., G.A. Sarria & Crous,
comb. & stat. nov.
Basionym. Cercospora palmicola f. stilbacea Moreau, Rev. Mycol. 12:
38. 1947.
≡ Helminthosporium stilbaceum (Moreau) S. Hughes, Mycol. Pap. 48:
38. 1952.
≡ Exosporium stilbaceum var. stilbaceum (Moreau) M.B. Ellis, Mycol.
Pap. 82: 38. 1961.
= Exosporium stilbaceum var. macrosporum Subramon. & V.G. Rao,
Journal of the Annamalai University, part B, Sciences 29: 404. 1971.
Mycelium superficial and immersed, hyphae hyaline to pale
brown, smooth, branched, septate. Conidiophores erect, brown
to red-brown, synnematous, septate, compacted, 620–1400 ×
19–54 µm, individual hyphae 3–4 µm wide. Conidiogenous
cells mono- or polytretic, integrated, determinate, terminal,
cylindrical, 31–67 × 4.5–7 µm, straight or curved at the apex.
Conidia catenate in easily disarticulating chains, obclavate,
subcylindrical, occasionally bifurcate, medium brown, 26–83
× 7–10 µm, (1–)3–5(–6)-distoseptate, striate-wall, hilum darkened, thickened and refractive.
Culture characteristics — Colonies on OA, reaching 9–12
mm diam after 1 wk at 25 °C in the dark. Velvety, with concentric
rings and some black spots in the agar, olivaceous to green
olivaceous, margin entire, white; reverse grey olivaceous. Colonies on MEA, reaching 20–27 mm diam after 1 wk at 25 °C in
the dark. Velvety, elevate, dark brick, margin irregular, reverse
vinaceous buff.
Specimen examined. coloMbia, Barrancabermeja, CENIPALMA, on
leaves of Elaeis oleifera, May 2013, G. Andrea Sarria (culture CPHmZC-01,
ITS sequence GenBank KX228298.1, LSU sequence GenBank KX228355.1,
MycoBank MB816989).
Notes — This species was initially introduced as Cercospora
palmicola f. stilbacea by Moreau (1947) as a ‘form’, different
from C. palmicola due to the presence of a ‘coremium’. Later
it was transferred to Exosporium (Ellis 1961). Nevertheless,
the generic placement of this species is doubtful in Cerco
spora or Exosporium and was tentatively accepted in Hel
minthosporium (Braun et al. 2014). Helminthosporium as well
as Helminthosporiella are asexual genera in Massarinaceae
with polytretic conidiogenous cells and distoseptate conidia.
However, they are molecular and morphologically different.
Helminthosporiella shows terminal conidiogenous cells and
catenate conidia and species of Helminthosporium have both
terminal and intercalary conidiogenous cells and solitary conidia. Another genus morphologically similar is Corynespora.
But Helminthosporiella differs from Corynespora in having
polytretic and sympodial, instead of monotretic and percurrent
conidiogenous cells. Unfortunately, it was not possible to propose a formal epi- or neotypification, since the geographical
origin of the specimen examined was not the same as described
in the protologue (Democratic Republic of the Congo).
ITS. Based on a megablast search of NCBIs GenBank nucleotide database, the closest hits using the ITS sequences are
Helminthosporium velutinum (GenBank JN198435; Identities
= 446/480 (93 %), Gaps = 5/480 (1 %)), Helminthosporium
sp. (GenBank KJ877647; Identities = 447/480 (93 %), Gaps =
9/480 (1 %)) and Helminthosporium sp. (GenBank JN662484,
Identities = 496 /552 (90 %), Gaps = 16/552 (2 %)).
LSU. Based on a megablast search of NCBIs GenBank nucleotide database, the closest hits using the LSU sequences
are Corynespora leucadendri (GenBank KF251654, Identities
= 820/840 (98 %), Gaps = 7/840 (0 %)), Corynespora olivacea
(GenBank JQ044448, Identities = 831/ 858 (97 %), Gaps =
6/858 (0 %)) and Byssothecium circinans (GenBank AY016357;
Identities = 830/858 (97 %), Gaps = 7/858 (0 %)) (MycoBank
supplementary data).
Colour illustrations. Nursery of Elaeis oleifera in CENIPALMA, Colombia;
Helminthosporiella stilbaceae: synnemata, conidiogenous cells and conidia.
Scale bars = 10 µm.
Margarita Hernández-Restrepo & Pedro W. Crous, CBS-KNAW Fungal Biodiversity Centre, P.O. Box 85167, 3508 AD Utrecht, The Netherlands;
e-mail: m.hernandez@cbs.knaw.nl & p.crous@cbs.knaw.nl
Greicy Andrea Sarria, Corporación Centro de Investigación en Palma de Aceite (CENIPALMA), Colombia; e-mail: gsarria@cenipalma.org
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
438
Persoonia – Volume 36, 2016
Penidiellopsis radicularis
439
Fungal Planet description sheets
Fungal Planet 460 – 4 July 2016
Penidiellopsis Sandoval-Denis, Gené, Deanna A. Sutton & Guarro, gen. nov.
Etymology. Named after its morphological resemblance to the genus
Penidiella.
Classification — Teratosphaeriaceae, Capnodiales, Dothi
deomycetes.
Conidiophores differentiated, solitary, erect, straight to geniculate-sinuous, rarely branched, pale to medium brown, smoothand thick-walled. Conidiogenous cells integrated, terminal
or intercalary, pale to medium brown, smooth, mono- and
polyblastic, giving rise to one or more sets of ramoconidia,
scars truncate, slightly darkened, unthickened and not refractive. Ramoconidia 0–1-septate, obovoid, ellipsoid or slightly
clavate, pale to medium brown, smooth- and thick-walled,
apical part with denticle-like loci, basal scar flattened, slightly
darkened, unthickened and not refractive. Conidia in branched
acropetal chains, 0-septate, obovoid, ellipsoid or limoniform,
pale to medium brown, smooth, thick-walled, with conidial scars
truncate or protuberant, somewhat darkened, unthickened and
not refractive.
Type species. Penidiellopsis radicularis Sandoval-Denis, Gené, Deanna
A. Sutton & Guarro.
MycoBank MB815361.
Penidiellopsis radicularis Sandoval-Denis, Gené, Deanna A. Sutton & Guarro, sp. nov.
Etymology. Named after its root-like growth pattern in culture media.
Mycelium superficial and immersed, composed of septate,
branched, pale brown, smooth to finely verruculose and thinwalled hyphae, 1.5–4.5 μm wide. Conidiophores straight or
geniculate, septate, slightly constricted at the septum, 40–150
× 3.5–5 μm, pale to medium-brown, usually darkening at the
medial portion, smooth-, thick-walled. Conidiogenous cells
terminal or intercalary, 12–18 × 4–5 μm, with one or several conidiogenous loci, 1–2.5 μm wide. Ramoconidia 0(–1)-septate,
ellipsoid, obovoid or somewhat clavate, 7–11 × 3–4 μm, pale
to medium brown, smooth and thick-walled. Conidia 0-septate,
obovoid or limoniform, 5–9 × 3–4 μm, pale brown, smooth and
thick-walled, with protuberant conidial scars.
Culture characteristics — (in the dark, 25 °C after 14 d),
colonies on PDA attaining 6–7 mm diam, dark green (30F3/F8)
(Kornerup & Wanscher 1978), erumpent and folded, velvety;
reverse dark green (30F3) to black. On SNA attaining 4–8 mm
diam, olive grey to olive (3F2/F8), flat, velvety; reverse olive
(3F7/F8). On OA attaining 6–8 mm diam, dark green (30F8)
to black, flat, velvety; reverse dark green (30F8).
Typus. usa, South Carolina, West Columbia, from human nail, date unknown, D.A. Sutton (holotype CBS H-22389, culture ex-type CBS 140695 =
UTHSC DI-13-256 = FMR 13369; ITS sequence GenBank LN834441, LSU
sequence GenBank LN834445, MycoBank MB815362).
Notes — The genus Penidiellopsis (Ps.) is similar to Peni
diella (Pa.), however, both genera are clearly differentiated genetically and morphologically. While Penidiella produces penicillate branched conidiophores, those of Penidiellopsis are mostly
unbranched, although its conidial chains exhibit a continuous
bi- or trifurcating elongation pattern (Crous et al. 2007a). The
monotypic genus Xenopenidiella exhibits also similar morphological features, however, is genetically distinct and produces
dimorphic conidiophores with loosely branched apices (Quaedvlieg et al. 2014).
Our phylogenetic results showed that Ps. radicularis is closely
related to Pa. aggregata and Pa. drakensbergensis, two species not included in Penidiella s.str. (sensu Quaedvlieg et al.
2014) (for phylogenetic tree, see MycoBank). However, the
new genus Penidiellopsis is genetically well-delimited, and
also differs from the latter two species by its shorter ramoconidia (vs 8–15 μm and 10–15 μm long in Pa. aggregata and
Pa. drakensbergensis, respectively) and its wider, aseptate
intermediate and terminal conidia.
Based on a megablast search of NCBIs GenBank nucleotide
database, the closest hit using the ITS sequence is Pa. aggre
gata CBS 128772 (GenBank JF499842; Identities= 466/508
(92 %), Gaps = 11/508 (2 %)), followed by Pa. drakensbergen
sis CPC 19778 (GenBank NR_111821; Identities = 469/519
(90 %), Gaps = 23/519 (4 %)) and Teratosphaeria agapanthi
CBS 129064 (GenBank JF770456; Identities = 435/509 (89 %),
Gaps= 18/509 (3 %)). Closest hits using the LSU sequence
were to Pa. aggregata CBS 128772 (GenBank JF499862; Identities = 539/551 (98 %), Gaps = 0/551 (0 %)), Pa. drakensberg
ensis CPC 19778 (GenBank KC005792; Identities = 536/551
(97 %), Gaps = 0/551 (0 %)) and Teratosphaeria macowanii
CPC 1872 (GenBank EU019254; Identities = 534/551 (97 %),
Gaps = 0/551 (0 %)).
Colour illustrations. USA, South Carolina, view of the Gervais Street
Bridge (image credit: Wikimedia commons); conidiophores, conidiogenous
cell bearing conidia in branched chains. Scale bars = 5 µm.
Marcelo Sandoval-Denis, Josepa Gené & Josep Guarro, Mycology Unit, Medical School and IISPV, Universitat Rovira i Virgili (URV), Sant Llorenç 21,
43201 Reus, Tarragona, Spain; e-mail: msandovaldenis@gmail.com, josepa.gene@urv.cat & josep.guarro@urv.cat.
Deanna A. Sutton & Nathan P. Wiederhold, Fungus Testing Laboratory, Department of Pathology, University of Texas Health Science Center,
7703 Floyd Curl Dr., San Antonio, Texas 78229-3900, USA; e-mail: suttond@uthscsa.edu & wiederholdn@uthscsa.edu
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
440
Persoonia – Volume 36, 2016
Ganoderma ecuadoriense
441
Fungal Planet description sheets
Fungal Planet 461 – 4 July 2016
Ganoderma ecuadoriense W.A. Salazar, C.W. Barnes & Ordóñez, sp. nov.
Etymology. Name reflects the geographical origin from which the fungus
was collected.
Classification — Ganodermataceae, Polyporales, Agarico
mycetes.
Basidiomata annual, flabelliform, pileate, pileus 21.6 × 21.8
mm, surface glabrous, woody, reddish brown, laccate, upper
surface covered by cinnamon coloured powder of deposited
basidiospores. Basidiospore surface smooth, white when fresh
and dark brown when dry, pores 5–7 per mm, round, thick walls.
Stipe missing from sample, but lateral. Hyphal system dimitic,
skeletal hyphae yellow to pale brown, 3.5–5.5 μm wide, end
in ramifications, generative hyphae thin-walled, hyaline to pale
yellow, 1–2.5 μm wide. Hyphae faintly amyloid when dispersed
and slightly dextrinoid when in masses with Melzer. Cuticule
cells club-like, slight amyloid reaction to 5 % KOH and Melzer.
Resin deposits between the trama and cuticle. Basidia not observed. Basidiospores hyaline to pale yellow, truncated, 8–10.5
× 4.5–7 μm, no reaction to 5 % KOH or Melzer.
Notes — Morphological identification using the Neotropical
Polyporaceae key (Ryvarden 2004), revealed G. ecuadoriense
to be very similar to G. perzonatum. However, based on a
Blastn ITS sequence comparison, after trimming the 18S and
28S sequences (Schoch et al. 2014), the highest similarities
were obtained with G. orbiforme from Brazil, and G. cupreum,
G. mastoporum and G. fornicatum from China. The sequence
of Ganoderma sp. VPB202 from Brazil is actually identical,
but has a low query score due to the discrepancy in sequence
length, missing roughly 25 bases at the 3’ end of ITS2. There
were seven consistent differences, three in ITS1 and four in
ITS2, between G. ecuadoriense, collected in the North-western
Amazon basin in Ecuador, and G. orbiforme, collected in the
South-eastern Amazon in Brazil. Twenty sequences, 16 for
G. ecuadoriense and four for G. orbiforme were used in the DNA
alignment analysis. The Ganoderma sp. VPB202 sequence
suggests G. ecuadoriense occurs throughout the Amazon
basin, but due to the missing bases of the ITS2 sequence, this
is somewhat speculative.
Typus. ecuador, Orellana Province, Yasuní Research Station, on decaying
wood, Mar. 2013, A. Salazar (holotype QCAM3430, ITS sequence GenBank
KU128524, LSU sequence GenBank KX228350, TreeBASE Submission ID
18454, MycoBank MB816866).
G . perzonatum S P 445984 B R A
G . perzonatum S P 445985 B R A
G . cupreum G anoTK 7 C MR
83
G . orbiforme UR M83335 B R A
G . orbiforme UR M83336 B R A
92
100
G . orbiforme UR M83334 B R A
G anoderma sp. VP B 202 B R A
92
G . ecuadoriens e P oly-2-4 E C U
(t
G . ecuadoriens e AS L 799 E C U (type)
G . ecuadoriens e P MC 126 E C U
79
G . fornicatum TNMF 0009926 C HN
G . cupreum HMAS 130804 C HN
Phylogenetic analysis was done using the Maximum Likelihood
plugin PHYML in Geneious v. 7.1 (http://www.geneious.com,
Kearse et al. 2012), and the substitution model HKY85 determined by jModelTest (Posada 2008) according to Corrected
Akaike Information Criterion (AICc). One hundred bootstrap
replicates were used. Included in the analysis were representative species found in the Blastn search, plus G. perzonatum
sequences because of its morphological similarity. Sample
nomenclature: species name, isolate number, three letter United
Nations country code: AUS = Australia, BRA = Brazil, CHN =
China, CMR = Camaroon, ECU = Ecuador and MYS = Malaysia.
G . mastoporum TNMF 0018835 C HN
91
G . mastoporum TNMF 0018783 C HN
G . cupreum HMAS 99399 C HN
G . fornicatum B C R C 35374 C HN
69
G . cupreum DF P 4336 AUS
G . fornicatum TNMF 0010592 C HN
G . mastoporum F R IM98 MY S
G . cupreum DF P 3896 AUS
0.02
Colour illustrations. Ecuador, Yasuni National Park rain forest; basidiocarps, skeletal and generative hyphae, basidiospores. Scale bars = 10 µm.
Washington A. Salazar, Maria E. Ordóñez, & Cristina Toapanta, Escuela de Ciencias Biológicas, Pontificia Universidad Católica del Ecuador,
Av. 12 de octubre 1076 y Roca, Quito, Ecuador;
e-mail: andres_salazar89@hotmail.com, meordonez@puce.edu.ec & ceta_333@hotmail.com
Charles W. Barnes, Instituto Nacional Autónomo de Investigaciones Agropecuarias, Estación Experimental Santa Catalina,
Panamericana Sur Km 1, Sector Cutuglahua, Pichincha, Ecuador;
e-mail: cbarnes333b@gmail.com
Paul Gamboa, Universidad Central del Ecuador, Facultad de Medicina, Carrera de Ciencias Biológicas y Ambientales, Av. América, Quito, Ecuador;
e-mail: paulgamboativi@hotmail.com
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
442
Persoonia – Volume 36, 2016
Blastobotrys meliponae
443
Fungal Planet description sheets
Fungal Planet 462 – 4 July 2016
Blastobotrys meliponae R.N. Barbosa, Boekhout, G.A. Silva, Souza-Motta &
N. Oliveira, sp. nov.
Etymology. me.li,po’nae. N.L. gen. n. meliponae, of the bee genus Meli
pona.
Classification — Trichomonascaceae, Saccharomycetales,
Saccharomycetes.
Hyphae thin, 1–1.5 µm wide, septate, branched, hyaline. Chla
mydospores globose or subglobose, terminal or intercalary, up
to 5.5 µm diam. Conidiophores erect, sympodially branched,
130–260 µm long or more, 2.0–2.6 µm wide, simple or branched, tapering upwards, producing 1–2 joint conidiogenous cells.
Conidiogenous cells are discrete, shortly pedicellate, globose
to subglobose, (1.6–)2.5–3(–4) µm, with one seta. The conidiogenous cells separate easily from the conidiophores and
are densely covered with conidia (up to 15 conidia, but mostly
6–13). Setae straight, sometimes slightly curved, narrowing
towards the apex, 1-septate, not deciduous, 55–124 µm long
or more (260 µm after 18 d of growth). Conidia holoblastic,
globose, smooth, sessile, 1–1.5 (can be up to 2) µm diam; may
form directly on 1–2 µm diam hyphae and below the tip of conidiophores. In yeast-like colonies, growth with budding observed
on hyphae. Glucose, galactose and sucrose are fermented, and
maltose, raffinose and xylose are not fermented. L-Arabinose,
D-xylose, acetate, glucuronate, erythritol, D-galactose, D-glucose, lactose, D-maltose, L-sorbose and L-rhamnose are assimilated. D-glucosamine, glycerol, raffinose, and mellibiose
are not assimilated. Nitrate and citrate are not assimilated.
Does not hydrolyse urea.
Culture characteristics — Colonies on 5 % malt extract agar
(5 % ME) at 25 °C grow slowly, white with irregular margins,
delicately downy, cerebriform, opaque, with light brownish reverse; 8 mm after 7 d. Colonies on yeast malt agar (YM) similar
to those on 5 % ME but with light yellowish reverse; 11 mm in
7 d. Colonies on restricted growth agar (RG) similar to those on
5 % ME, but differ by plane colonies, and a colourless reverse;
5 mm in 7 d. Colonies at 27 °C, 28 °C, 30 °C and 37 °C were
similar to colonies at 25 °C. At 10 °C no growth was observed.
Notes — Based on phylogenetic analyses using only sequences of the D1/D2 domains, the three isolates formed a
clade with B. proliferans, but the sequences showed only 91 %
identity with the LSU sequences of that species in a BLASTn
analysis, indicating that these isolates represented a new
yeast species of Blastobotrys. Members of Blastobotrys with
a high similarity of the LSU rDNA D1/D2 domains to the new
species were: B. attinorum (GenBank GU373758; 92 %), B. pro
liferans (GenBank EF584541; 91 %) and B. nivea (GenBank
DQ442690; 90 %). Blastobotrys meliponae differs from B. nivea
in having sympodially branched conidiophores, lacking budding
cells and chlamydospores, but with lateral conidia forming
directly on the hyphae. The species can be distinguished from
B. aristata by the size of the conidiogenous cells (3–8 × 4.5–9
µm), conidiophore branching, number and size (100 µm) of
setae, absence of lateral conidia formed directly on the hyphae,
absence of chlamydospores and growth at 37 °C. Blastobotrys
proliferans has a different branching of the conidiophores, conidiogenous cell size (3–4.5 × 4.5–7 µm), setae with a spathulate
apex in older cultures and presence of distinct refraction bodies
in the conidiogenous cells.
Typus. brazil, Recife, Pernambuco, isolated from honey of the bee Meli
pona scutellaris collected in Atlantic Forest (S8°7'30" W34°52'30") (metabolically inactive culture, holotype URM 7224, isotype CBS 14100, ITS
sequences GenBank KT448719, KT448720, KT448721, LSU sequences
GenBank KR779215, KR779216, KR779217, MycoBank MB812601).
Colour illustrations. Bees and pot honey in the nest of Melipona scutel
laris; chlamydospores, hyaline conidia on hyphae and conidiophores on YM
agar for 7 d at 28 °C. Scale bars = 10 µm.
Renan N. Barbosa, Gladstone A. Silva & Neiva T. Oliveira, Departamento de Micologia Prof. Chaves Batista,
Universidade Federal de Pernambuco, Recife, Brazil; e-mail: renan.rnb@gmail.com, gladstonesilva@yahoo.com, netinti@hotmail.com
Cristina M. Souza-Motta, URM Culture Collection, Recife, Brazil; e-mail: cristina.motta@ufpe.br,
Teun Boekhout, CBS-KNAW Fungal Biodiversity Centre; Utrecht, The Netherlands; e-mail: t.boekhout@cbs.knaw.nl
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
444
Persoonia – Volume 36, 2016
Russula intervenosa
445
Fungal Planet description sheets
Fungal Planet 463 – 4 July 2016
Russula intervenosa S. Paloi, A.K. Dutta & K. Acharya, sp. nov.
Etymology. ‘Intervenosa’ is the Latin transliteration of ‘intervenose’, referring to the pattern of lamellae.
Classification — Russulaceae, Russulales, Agaricomycetes.
Pileus 26–49 mm diam, convex to broadly convex when young,
becoming infundibuliform to applanate with a central depression in age, surface smooth when young, becoming cracked at
maturity, semi moist, dark red (10C8) to brownish red (10D8)
at center, pastel red (10A5) to dull red (10B4) towards margin,
unchanging with NH4OH and FeSO4, pale orange (5A3) to light
orange (5A4) with KOH, red (9B8) to brownish red (9C8) with
sulfovanillin (SV), red (9A7) with guaiacol; margin translucent
striate; context very thin (< 1 mm), white (1A1), no colour change
when exposed, turns whitish yellow (8A2) to pale yellow (8A3)
with FeSO4, reddish brown (9D8) with SV. Lamellae adnexed,
4–5 mm broad, regular, with intervenose to reticulate like appearance, lamellulae none, dull yellow (3B3), edge even, concolorous, light yellow (4A4) to yellowish orange (4A6) with
FeSO4, pale yellow (3A3) with KOH, reddish brown (9D-E8)
with SV, light brown (5D4) to yellowish brown (5E4) with 10 %
phenol. Stipe central, 8 –15 × 4 – 6 mm, tapered toward the
base, smooth, semi moist, greyish red (10C5) to brownish red
(10D6), white (1A1) towards extreme base, unchanging on
brushing, light brown (7D7) to brown (7E7) with 10 % phenol
and SV, red (9A6) to brownish red (9C7) with guaiacol; context
hollow, white (1A1), unchanging after brushing. Odour and taste
mild. Spore print cream. Basidiospores (6.5–)7–7.5 –8.0(–9)
× (6–)6.5– 6.7–7(–7.5) µm, Q = 1.07–1.12 –1.19, subglobose,
hyaline, ornamentation amyloid, composed of high (0.6–0.9
µm) and low (0.1–0.4 µm) ridges with irregularly interrupted
margin that are aligned or connected to give nearly complete
or partial reticulum to reticulate-winged fashion; suprahilar
plage amyloid, up to 2 µm long. Basidia (28–)30–40(–43) ×
10.5–12(–14) µm, 4-spored, clavate to subclavate, thin-walled,
oil droplets present when viewed with KOH; sterigmata 3.5–4.5
× 1 µm. Subhymenium pseudoparenchymatous. Lamellar trama
composed of sphaerocytes and hyphal cells, sphaerocytes
measuring c. 22–39 × 18–29 µm. Hymenial cystidia c. (26–)
32–39(–43) × (4.5–)5.5–7(–9) µm near gill edge, cylindrical
with pointed towards apex, filled with cytoplasmic contents; on
gill sides c. 29–34(–39) × 10–12.5(–14.5) µm, appendiculate
to fusiform, thin-walled, oil droplets present when viewed with
KOH. Pileipellis orthochromatic in cresyl blue, sharply delimited from the underlying sphaerocytes of the context, distinctly
divided into a 43–50 µm deep subpellis composed of loosely
arranged, less gelatinized, measuring 2.5–4.5 µm diam, hyaline, thin-walled hyphae and less gelatinized, c. 47– 64 µm
deep suprapellis composed of interwoven, measuring c. 36–64
× 2.5–4 µm, erect to suberect, thin-walled, hyaline hyphae of
subulate to pointed hyphal apex and up to five round cells,
Colour illustrations. India, West Bengal, vegetation cover of the collection site (background). Left column: field photograph of the basidiocarp,
fresh basidiocarp showing lamellae, basidia, SEM microphotograph of the
basidiospores; right column: cheilocystidia, pleurocystidia (all from holotype).
Scale bars = 10 mm (basidiocarps), 20 µm (microscopic structures), 1 µm
(basidiospore).
measuring 39–47 × 2.5–4.5 µm at base; pileocystidia absent.
Stipitipellis up to 42–51 µm thick, composed of loosely arranged
subulate hyphae with pointed apex, up to 2.5–4.5 µm broad,
often branched, hyaline, thin-walled and caulocystidia measuring 26–32 × 5.5–6.5 µm, subcylindrical to subclavate to with
pointed to moniliform apex, filled with cytoplasmic contents.
Stipe trama composed of sphaerocytes.
Typus. india, West Bengal, Paschim Midnapur, Lodhasuli forest, ectomycorrhizal with Shorea robusta (Dipterocarpaceae), 13 July 2014, S. Paloi
(holotype CAL-1272, ITS sequence GenBank KT824241, LSU sequence
GenBank KU928135, MycoBank MB814593).
Notes — The combination of macro- and micromorphological
characters undoubtedly place R. intervenosa in the subgenus
Amoenula (Sarnari 1998, Das & Sharma 2005).
Based on a megablast search of NCBIs GenBank nucleotide
database, the closest hits using the ITS1-5.8S-ITS2 sequence
had highest similarity with R. violeipes (GenBank KF361797;
Identities 512/548 (93 %), Gaps = 12/548 (2 %)) and R. ma
riae (GenBank EU819426; Identities 481/529 (91 %), Gaps =
15/529 (2 %)). However, R. violeipes differs from the newly
described species by its more variegated pileus colours, much
longer stipe (up to 70 mm) and differently sized basidiospores
(6–8 × 6.5–9 µm; Quélet 1898). Russula mariae has a more
fleshy pileus with wider cap (up to 5 mm), with flushed pink or
purplish surface that does not react with SV and slowly turns
blue green with guaia (Peck 1872, Bills & Miller 1984). (MycoBank supplementary data.)
Considering the overall size and ornamentation of the basidiospores and nature of pileipellis, our species is related to
R. amoena and R. amoenicolor. However, R. amoena, the type
species of the subgenus, can be distinguished from the present
species in having a pileus coloured shades of purple or violet
with velvety cuticle, context that turns violet with phenol, much
longer and pruinose stipe (up to 50 mm), dichotomous split of
lamellae, presence of a sweetish taste and fragrance like fruit
(Quélet 1880, Romagnesi 1967, Sarnari 1998). Russula amoe
nicolor, originally described from Europe, has much larger (up
to 80 mm diam) and velvety pileus coloured purplish brown or
purple, and much longer stipe (up to 70 mm; Romagnesi 1967).
Among the New Zealand taxa, species with similar shape,
colouration of pileus with smaller stipe: R. miniata differs by
the presence of white coloured lamellae, larger basidiospores
(9.5–11.5 × 8–10 µm), absence of caulocystidia and habitat
under Nothofagus sp.; R. pudorina has a white coloured spore
print, larger basidiospores (8–10.5 × 7–9 µm), bitter taste and
habitat under Leptospermum spp. (McNabb 1973). Within the
same subgenus Amoenula, previous described species from
the moist deciduous to mixed subtropical forests of India include
R. mukteshwarica. But, R. mukteshwarica is more robust
(6.5–13 cm) and has a purple coloured pileus with light to brilliant or very greenish yellow centre, white coloured spore print
and basidiospores are somewhat larger (7.5–9.5 × 7.5–8 µm;
Das et al. 2005).
Soumitra Paloi, Arun Kumar Dutta & Krishnendu Acharya, Molecular and Applied Mycology and Plant Pathology Laboratory,
Department of Botany, University of Calcutta, 35, Ballygunge Circular Road, Kolkata-700019, West Bengal, India;
e-mail: soumitrabotany@gmail.com, arun.botany@gmail.com & krish_paper@yahoo.com
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
446
Persoonia – Volume 36, 2016
Crinipellis odorata
447
Fungal Planet description sheets
Fungal Planet 464 – 4 July 2016
Crinipellis odorata K.P.D. Latha & Manim., sp. nov.
Etymology. The name refers to the odoriferous basidiomata of this species.
Classification — Marasmiaceae, Agaricales, Agaricomy
cetes.
Basidiomata small to medium-sized, marasmioid, often in dense
tufts. Pileus 6 – 31 mm diam, truncately conical or conicoconvex with a small central depression when very young,
becoming campanulate to broadly campanulate, still with
a small central depression; surface dark brown (8F4, 8F5/
OAC635) or reddish brown (8F7/OAC636) at the centre and
on the squamules and brownish orange (6C5/OAC652) or
light brown (6D5/OAC659) elsewhere, not hygrophanous, not
striate, appressed- to slightly recurved-squamulose all over;
margin initially incurved, becoming decurved to almost reflexed
with age, finely appendiculate, crenate or somewhat wavy.
Lamellae adnexed, crowded, yellowish white (4A2/OAC815)
when very young, becoming greyish yellow (4B4/OAC806),
up to 2 mm wide, with lamellulae of 3 lengths; edge crenate,
concolorous with the sides. Stipe 39–120 × 2–4 mm, central,
terete, or slightly compressed, tapering towards the base,
hollow, slightly flexuous towards the base, insititious; surface
concolorous with the pileus, appressed- to slightly recurvedsquamulose all over, densely so towards the apex; base deeply
rooted. Rhizomorphs absent. Context up to 2 mm thick, yellowish brown. Odour strong, unpleasant. Taste not distinctive.
Basidiospores 5–8 × 5–7 (6.5 ± 0.77 × 5.9 ± 0.61) µm, Q =
0.86–1.3, Qm = 1.11, subglobose to almost globose, smooth,
thin-walled, inamyloid. Basidia 23–33 × 6–9 µm, sparse clavate, hyaline, thin-walled, 4-spored; sterigmata up to 4 µm long.
Basidioles 28 – 48 × 6 –12 µm, abundant, fusoid or clavate,
thin-walled, hyaline. Lamella edge sterile with copious cheilocystidia. Cheilocystidia 22–65 × 7–19 µm, versiform: oblong,
clavate, cylindrical, cylindrical with a median constriction,
flexuous, nodulose-diverticulate, sometimes capitate, thin- to
slightly thick-walled, hyaline or pale yellow. Pleurocystidia
absent. Lamellar trama subregular; hyphae 3 –12 µm wide,
thin-walled, hyaline or pale yellow, inamyloid. Pileus trama
subregular to interwoven; hyphae 6–15 µm wide, thick-walled
(up to 2 µm thick), with a pale yellow wall pigment, dextrinoid.
Pileipellis composed of tufts of fasciculate hairs arising from a
hypotrichium; hypotrichial hyphae 8–18 µm wide, thick-walled
(up to 1.5 µm thick), with pale yellow wall and dense, spiral
encrusting pigments; pileal hairs 500–1200 × 5–7.5 µm or more,
unbranched, cylindrical or sinuous-cylindrical with an obtuse
apex, thick-walled (up to 2 µm thick), with a yellowish brown
wall pigment, dextrinoid, yellowish brown in KOH. Stipitipellis
composed of clusters of hairs arising from a hypotrichium with
flairing-out cystidioid terminal cells; hypotrichial hyphae 3–7 µm
wide, thick-walled (up to 1 µm thick), with pale yellow wall and
minutely, encrusting pigments; stipitipellis hairs 47.5–452.5 ×
7.5–12.5 µm, sinuous-cylindrical, thick-walled (up to 3 µm thick),
with a pale brown or yellowish brown wall pigment, dextrinoid,
pale yellowish brown in KOH; terminal cells cystidioid 32–95
× 10–16 µm, clavate, cylindrical-clavate or irregular in outline,
Colour illustrations. India, Kerala State, Wayanad District, Wayanad
Wildlife Sanctuary, type locality; basidiomata, basidiospores, cheilocystidia,
terminal elements of stipitipellis. Scale bars = 10 mm (basidiomata), 10 µm
(microscopic structures).
dextrinoid, thick-walled (up to 2 µm thick), with a pale yellowish
brown wall pigment. Caulocystidia absent. Clampconnections
observed on all hyphae except at the base of basidia.
Habit, Habitat & Distribution — In dense caespitose clusters
or in small groups, attached to the bark at the base of a living
Mytragyna parviflora tree as well as deeply rooted in the nearby
soil. Known only from the type locality in Kerala State, India.
Typus. india, Kerala State, Wayanad District, Tholpetty, Wayanad Wildlife
Sanctuary, 6 July 2013, K.P. Deepna Latha (holotype CAL 1240, ITS sequence GenBank KT952521, MycoBank MB814919).
Notes — Characters such as a brown pileus not reacting
with KOH, a long, central stipe, long, thick-walled, dextrinoid
hairs on both the pileipellis and the stipitipellis and subglobose
basidiospores indicate that the present species belongs to
Crinipellis sect. Crinipellis subsect. Macrosphaerigerae (Singer
1976, 1986). The key to the species of Crinipellis by Singer
(1953) leads C. odorata into C. macrosphaerigera, a Brazilian species, as both species have somewhat similar pileus
surfaces, adnexed lamellae, subglobose basidiospores, versiform cheilocystidia and association with living trees. However,
C. macrosphaerigera has smaller basidiomata with a strawcoloured pileus and stipe, distant lamellae, larger basidiospores
(12.5–14 × 9.5–11.5 µm), a heterogeneous lamella edge and
an indistinct odour. Crinipellis podocarpi, a species originally
described from Argentina (Singer 1976) and subsequently from
Mexico (Bandala et al. 2012) and belonging to sect. Crinipellis of
subsect. Stipitarinae, seems to be somewhat close to C. odorata
in having basidiospores of almost similar size and shape. However, C. podocarpi differs in all other macro- and microscopic
characters. Comparison of the ITS sequence (CAL 1240: 754
bp) generated from C. odorata with the nucleotide sequences
of taxa available in GenBank suggest that C. odorata has a
distinct ITS sequence. In a megablast search of the GenBank
database using ITS sequence of the species, the closest hit
was C. floccosa (GenBank KJ698642; Identities = 685/762
(90 %), Gaps = 32/762 (4 %)) followed by C. zonata (GenBank
FJ167659; Identities = 662/741 (89 %), Gaps = 35/741 (4 %)).
Crinipellis floccosa, a species recently described from China
(Xia et al. 2015), shares a few features such as a reddish brown,
squamulose pileus, somewhat similarly-coloured lamellae,
similar-sized basidiospores ((5.5–)6–8 × 4–4.5(–5)), somewhat similar-sized cheilocystidia, the absence of pleurocystidia
and a similar pileipellis structure with C. odorata. Crinipellis
floccosa is distinguished, however, by its smaller basidiomata,
free lamellae with a denticulate margin, a differently-coloured,
equal, tomentose-pilose stipe, ellipsoid to broadly ellipsoid or
somewhat amygdaliform basidiospores, scattered cheilocystidia, the hairs of both the pileus and stipe exhibit greenish yellow
in KOH and a non-distinctive odour. Crinipellis zonata, a species
reported from North America (Redhead 1989), Europe (Antonín
& Noordeloos 1997, 2010) and the Republic of Korea (Antonín
et al. 2009), has similar looking basidiomata with almost similar
colour, crowded lamellae, somewhat similar morphology of
cheilocystidia, lamellae devoid of pleurocystidia, similar pileipellis and stipitipellis structure and clamped hyphae. However,
C. zonata has smaller basidiomata, a tomentose pileus with
an inflexed margin, differently attached, pale cream lamellae,
a hairy stipe, much narrower (3–4 μm) and cylindrical-ellipsoid
basidiospores, smaller pileipellis hairs and an indistinct odour.
(MycoBank supplementary data.)
K.P. Deepna Latha & Patinjareveettil Manimohan, Department of Botany, University of Calicut, Kerala, 673 635, India;
e-mail: pmanimohan@gmail.com & deepnalathakp@gmail.com
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
448
Persoonia – Volume 36, 2016
Corynascus citrinus
449
Fungal Planet description sheets
Fungal Planet 465 – 4 July 2016
Corynascus citrinus Giraldo & Crous, sp. nov.
Etymology. From Latin citrinus, citrine or lemon-yellow, referring to the
yellow pigment produced in vitro.
Classification — Chaetomiaceae, Sordariales, Sordariomy
cetes.
Ascomata cleistothecial, submerged or embedded in the aerial
mycelium, spherical, brown, 44–83 μm diam, surface of textura
epidermoidea, striated. Paraphyses absent. Asci evanescent,
subglobose, unitunicate, thin-walled. Ascospores unicellular,
broadly fusiform, thick- and smooth-walled, with germ pores
at both ends, 9–12 × 6–8 μm, hyaline becoming brown when
mature. Conidiophores absent or poorly differentiated. Conidia
growing directly on undifferentiated hyphae, lateral, sessile,
occasionally on short stalk, globose, 5–7 μm diam, hyaline,
thick-walled, tuberculate.
Culture characteristics — Colonies on PDA reaching 41–42
mm diam after 21 d at 25 °C, surface straw at centre and fuscous black at periphery, reverse fuscous black (Rayner 1970),
flat, floccose. On OA and MEA attaining 54–55 and 55–56
mm diam, respectively after 21 d at 25 °C, surface straw, flat,
granulose. Diffusible pure yellow pigment in all media tested.
Maximum likelihood (ML) tree based on partial sequences of
ITS, tef1 and rpb2 regions from reference and type strains of
Corynascus species. The alignment included 1 604 bp and
was generated with ClustalW under MEGA v. 6.06 (Tamura et
al. 2013). Tamura-Nei with Gamma distribution was used as
the best nucleotide substitution model. Myceliophthora lutea
(Chaetomiaceae, Sordariales) was used as outgroup taxon.
The new species is highlighted in bold face. Bootstrap support
values above 70 % are shown at the nodes. T Ex-type strain.
Accession numbers of ITS, tef1 and rpb2 sequences retrieved
from GenBank are in parentheses.
Typus. thailand, Nakhon Nayok province, Mueang Nakhon Nayok district,
Wang Takhrai waterfall, N14.330023° E101.307168°, 64 m above sea level,
from soil, 22 July 2015, A. Giraldo (holotype metabolically inactive culture
BCC 79098, ITS sequence GenBank KX262667, LSU sequence GenBank
KX228351.1, rpb2 sequence GenBank KX262668, tef1 sequence GenBank
KX262669, MycoBank MB816971).
Notes — The genus Corynascus (Chaetomiaceae, Sordari
ales), previously considered as synonym of Myceliophthora
(Van den Brink et al. 2012), was recently resurrected by MarinFelix et al. (2015). Currently, Corynascus contains five species
apart from C. citrinus, which are commonly isolated from soil
and characterised by their mesophilic habitat, ascomata cleistothecial of textura epidermoidea, ascospores with a germ
pore at each end, and a myceliophthora-like asexual morph
(Guarro et al. 2012). Corynascus citrinus is phylogenetically
closely related to C. sexualis and C. fumimontanus but can be
morphologically distinguished from the former species by the
presence of the asexual morph in culture, and from the latter
species by its smaller ascomata (50 –110 μm), ascospores
(13–17 × 7–9 μm) and conidia (6–10 μm) (Marin-Felix et al.
2015).
Corynascus verrucosus CBS 135878 (LK932695, LK932718, LK932734)
100
Corynascus verrucosus CBS 137791 (LK932699, LK932717, LK932732)
Corynascus verrucosus IMI 378522T (AJ224203, LK932723, LK932726)
Corynascus sepedonium CBS 632.67 (HQ871759, HQ871744, HQ871830)
99
Corynascus sepedonium IMI 378521 (AJ224201, LK932715, LK932730)
98
Corynascus sepedonium CBS 111.69T (HQ871751, HQ871734, HQ871827)
100
100
Corynascus novoguineensis NBRC 9556 (LK932698, LK932716, LK932731)
Corynascus novoguineensis CBS 359.72T (HQ871762, HQ871733, HQ871838)
4x
Corynascus citrinus BCC 79098T
88
Corynascus sexualis IMI 378520T (AJ224202, LK932714, LK932729)
Corynascus fumimontanus CBS 137294T (LK932694, LK932719, LK932733)
Myceliophthora lutea MUCL 10071 (LK932702, LK932711, LK932725)
Myceliophthora lutea MUCL 10070 (LK932701, LK932710, LK932724)
0.005
Colour illustrations. Thailand, Wang Takhrai waterfall, Mueang Nakhon
Nayok district, Nakhon Nayok province (photo: A. Giraldo); colony on PDA
after 21 d at 25 °C, ascoma with asci, detail of the peridium wall, immature
and mature ascospores, conidia. Scale bars = 10 µm.
Alejandra Giraldo & Pedro W. Crous, CBS-KNAW Fungal Biodiversity Centre, P.O. Box 85167, 3508 AD Utrecht, The Netherlands;
e-mail: a.giraldo@cbs.knaw.nl & p.crous@cbs.knaw.nl
J. Jennifer Luangsa-ard, Microbe Interaction Laboratory, BIOTEC, Thailand Science Park, Khlong Nueng,
Klong Luang Pathum Thani 12120, Thailand; jajen@biotec.or.th
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
450
Persoonia – Volume 36, 2016
Brunneocarpos banksiae
451
Fungal Planet description sheets
Fungal Planet 466 – 4 July 2016
Brunneocarpos Giraldo & Crous, gen. nov.
Etymology. Brunneus (Latin) = brown, and carpos (Greek) = fruit.
Classification — Mycocaliciaceae, Mycocaliciales, Eurotio
mycetes.
Ascomata apothecial, stipitate, growing intermingled among the
floral bracts on Banksia cones. Stipe straight or flexuous, mostly
branched. Capitulum brown, globose to subglobose. Para
physes not observed. Asci 8-spored, cylindrical, unitunicate.
Ascospores uniseriate, ellipsoidal to slightly fusiform, clavate,
1-celled, brown, smooth-walled. Mycelium consisting of septate, hyaline, smooth- and thin-walled hyphae. Asexual morph
producing dictyochlamydospores in chains, subhyaline, thickwalled, lateral or terminal.
Type species. Brunneocarpos banksiae Giraldo & Crous.
MycoBank MB816972.
Brunneocarpos banksiae Giraldo & Crous, sp. nov.
Etymology. Name reflects the host genus Banksia, from which the species
was isolated.
Ascomata apothecial, stipitate, growing intermingled among
the floral bracts on Banksia attenuata cones. Stipe dark brown,
shiny, flexuous, mostly branched two or three times at the apex,
1–2 mm long, 50–85 µm wide. Capitulum dark brown, globose
to subglobose with a funnel-shaped base, covered by hyaline
mycelium when older, 150–285 × 125–206 µm. Paraphyses
not observed. Asci 8-spored, cylindrical, unitunicate, 30–34 ×
4–5 µm. Ascospores uniseriate, ellipsoidal to slightly fusiform,
clavate, 1-celled, brown, thick- and smooth-walled, 4.5–7 ×
2–3.5 µm. Mycelium consisting of septate, hyaline, smooth- and
thin-walled hyphae, 1.5–2 µm diam. Conidiophores absent or
poorly differentiated. Dictyochlamydospores in chains, sessile
or with short subconical stalk, subhyaline to pale brown, thickand smooth-walled, lateral or terminal, 26 –60 µm long.
Culture characteristics — Colonies on MEA reaching 3–3.3
cm diam after 2 mo at 25 °C, vinaceous buff (Rayner 1970),
depressed at centre, floccose.
Typus. australia, Western Australia, S34°22'19.4" E118°1'33.6", on
Banksia attenuata (Proteaceae), 23 Sept. 2015, P.W. Crous (holotype
CBS H-22633, cultures ex-type CPC 29841 = CBS 141465, ITS sequence
GenBank KX262670, LSU sequence GenBank KX228352.1, MycoBank
MB816973); CPC 29070, CPC 29072, CPC 29435.
Notes — According to LSU and ITS analyses Brunneocar
pos banksiae belongs to Mycocaliciaceae (Mycocaliciales)
where four genera are currently accepted; Chaenothecopsis,
Phaeocalicium, Mycocalicium and Stenocybe. All produce apothecioid ascomata, usually with a tiny stalk and brown ascospores (Tuovila et al. 2011). These genera harbour resiniculous species, growing directly on exudate and/or on exudate-impregnated wood of different hosts, including Acer (Sapin
daceae), Mangifera (Anacardiaceae), Khaya (Meliaceae), Abies
(Pinaceae), Picea, Tsuga and Tilia (Malvaceae) (Rikkinen 2003,
Tuovila et al. 2011) and known species are lignicolous, algicolous or lichenicolous (Tuovila et al. 2014). So far no species
from these genera have been reported growing on Banksia,
Species growing in axenic culture produce a phialophora-like
(Chaenothecopsis shefflerae) or coelomycetous asexual morph
(Tibell 1991, Tibell & Vinuesa 2005), and in some cases, a
hyphomycetous asexual morph with ramoconidia in acropetal
chains (C. haematopus) (Tibell & Constantinescu 1991). This
morphological character differs from the new taxon proposed
here, which produces a chlamydospore-like asexual morph
in culture.
Colour illustrations. Australia, Western Australia, cones of Banksia at
tenuata (photo: P.W. Crous); stipitated apothecium, detail of the capitulum,
asci, ascospores, dictyochlamydospore. Scale bar = 10 µm.
Alejandra Giraldo & Pedro W. Crous, CBS-KNAW Fungal Biodiversity Centre, P.O. Box 85167, 3508 AD Utrecht, The Netherlands;
e-mail: a.giraldo@cbs.knaw.nl & p.crous@cbs.knaw.nl
Michael J. Wingfield, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria,
Pretoria 0002, South Africa; e-mail: mike.wingfield@up.ac.za
Treena I. Burgess & Giles E.St.J. Hardy, Centre for Phytophthora Science and Management, Murdoch University, 90 South Street,
Murdoch, WA 6150, Australia; e-mail: tburgess@murdoch.edu.au & g-hardy@murdoch.edu.au
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
452
Persoonia – Volume 36, 2016
Cercophora vinosa
453
Fungal Planet description sheets
Fungal Planet 467 – 4 July 2016
Cercophora vinosa A.N. Mill. & J. Fourn., sp. nov.
Etymology. The specific epithet refers to the purple colour in the outermost
layer of the ascomal wall.
Classification — Lasiosphaeriaceae, Sordariales, Sordario
mycetes.
Ascomata subglobose to broadly obpyriform with a stout conical
to hemispherical neck, 420–670 µm diam × 420–650 µm high,
erumpent through a thick, felty yellowish grey subiculum spreading widely over the substrate, gregarious to loosely clustered,
more rarely in contact; underlying wood discoloured greyish
brown or unaltered, not stained purple; subiculum encrusted
with sand particles, composed of pale brown, septate hyphae
1.5–3.5 µm wide, thin- to moderately thick-walled; neck papillate, broadly conical to rounded, sometimes undifferentiated,
80 –170 µm high, ostiolate, black, roughened or obscurely
sulcate. Ascomatal wall 34–56 µm thick on sides and at base
in longitudinal section, slightly thicker at apex, roughened, dark
purple gradually turning blackish, pseudoparenchymatous,
leathery, 3-layered: outermost layer 10–15 µm thick, present
on upper half of ascomata, fugacious, composed of several
rows of thin-walled, hyaline, angular cells containing a purple
substance that slightly fades in 10 % KOH and dissolves in
chloral-lactophenol; middle layer 20–30 µm thick, composed
of independent clusters of subcarbonaceous opaque cells,
breaking into small angular plaques upon pressure but lacking a
network of hyphae connecting the plaques as in cephalothecoid
walls; inner layer 14–22 µm thick, composed of flattened, thinwalled subhyaline cells. Ascomatal apex periphysate. Centrum
hyaline to yellowish. Paraphyses filiform, 4.5–8 µm wide at
base and occasionally slightly moniliform, tapering to 2–2.5
µm wide above asci, hyaline, thin-walled, abundant, septate,
unbranched, persistent. Asci unitunicate, cylindrical, 320–380 ×
19–23 µm, spore-bearing part fusoid-ventricose, apex rounded,
long-stipitate, stipe 100–160 µm long, often slightly sinuous,
with eight bi- to triseriate ascospores, apical ring double, refractive, 2.7–3 µm wide × 1–1.2 µm high, inamyloid, staining in blue
Waterman ink, subapical globule absent. Ascospores cylindrical, (54.5–)60.5–73.5(–79.5) × (3–)4–6(–7) µm (66.7 × 5 µm),
straight to slightly sigmoid, geniculate in lower quarter, hyaline,
aseptate, densely guttulate; bipolar appendages (27–)35–56
µm long, lash-like, 3–4 µm wide at base, centrally attached on
ascospores ends, readily staining in blue Waterman ink and
in aqueous nigrosin, sometimes granular at base, persistent;
ascospore becoming differentiated into an apical swollen head
and a basal tail while inside the ascus; head ellipsoid, 15.5–19 ×
8–9 µm, usually 1-septate, remaining hyaline, rarely pigmented;
tail 41–50 × 4–5 µm, obscurely 2–4-septate, hyaline, the lower
end swelling upon germination; germinating from upper and
lower ends, more rarely laterally before being released. Asexual
morph: Hyphae largely undifferentiated, 1.5–5 µm wide, thinwalled, hyaline to pale brown. Conidiogenous cells phialides,
commonly produced from pale brown hyphae as single terminal or several lateral phialides, delimited by a basal septum,
monophialidic or polyphialidic, cylindrical to obpyriform, 9–18 ×
2.5–4 µm at widest part, mostly pale brown, constricted below
the collarette, 1–1.5 µm just below the collarette; collarette
short, slightly flaring, inconspicuous, same colour as phialide.
Conidia subglobose to pyriform, truncate at base, 2.5–4.5 ×
1.5–2.5 µm (3.3 × 2.2 µm), hyaline.
Culture characteristics — Colonies (of holotype) slow-growing on all media, covering the PDA plates in 8 wk, 25–30 mm
diam after 8 wk on the WA and CMA plates, downy to silky on
WA and CMA, subfelty on PDA, hyaline on WA, hyaline to greyish yellow (4B3) on CMA, brown (5F8) and becoming greyish
red (7B5) at plug on PDA; margin even or wavy, appressed,
hyaline on WA and CMA, becoming olive brown (4F8) on PDA;
reverse same as the mat.
Typus. france, Ariège, Castelnau-Durban, Artillac stream, down-stream
from the marble quarry, c. 410 m elev., on decorticated branch of Salix sp.,
8 cm diam, partly submerged, 22 July 2014, J. Fournier, JF 14067 (holotype
ILLS 79802, cultures ex-type ANM Acc#840-1, -2, -3, -4, -5, deposited in CBS,
ITS-LSU sequence GenBank KX171944, beta-tubulin sequence GenBank
KX171942, MycoBank MB816935).
Other material examined. france, Ariège, Montségur, Le Lasset stream
flowing at the village, 880–890 m elev., on submerged decorticated wood of
Populus sp., soc. Amniculicola lignicola, 16 Nov. 2014, J. Fournier (JF 14156);
Illier, Laramade, Vicdessos stream, 630 m elev., on submerged wood of
Fraxinus excelsior, 25 Nov. 2014, J. Fournier (JF 14163); Rimont, Paletès,
Peyrau brook, c. 400 m elev., on submerged wood of Alnus glutinosa, 4 Dec.
2014, J. Fournier (JF 14170).
Notes — Cercophora vinosa is distinguished by its ascomata that possess a distinct purplish colour in the outermost
wall layer, asci with a double ring but lack a subapical globule,
long ascospores with lash-like appendages and aquatic habitat. Only two other species of Cercophora are known to have
violet-coloured ascomata, C. septentrionalis and C. caerulea.
Although these species also possess asci with a double ring
but no subapical globule and ascospores with long, lash-like
appendages, both have shorter ascospores (38–43 µm and
43–48 µm, respectively) and occur on dung. The ascomata in
C. septentrionalis are covered by brown, flexuous hairs, whereas a distinct dark purple to blackish blue subiculum surrounds
the ascomata in C. caerulea (Lundqvist 1972). Cercophora
vinosa occurs in a well-supported clade with C. solaris in which
it shares only a cephalothecoid-like ascomal wall and lack of a
subapical globule (Catania et al. 2011).
For phylogenetic tree, see MycoBank.
Colour illustrations. France, background photo of Artillac stream in the
Ariège region of south-western France; ascomata and ascomal section,
ascomal wall, ascus, ascospores, phialides, colony on CMA. Scale bars
= 100 µm (ascomata and ascomal section), 10 µm (ascomal wall, ascus,
ascospores and phialides), 10 mm (colony on CMA plate). (Photos: Jacques
Fournier).
Andrew N. Miller, University of Illinois Urbana-Champaign, Illinois Natural History Survey, 1816 South Oak Street, Champaign, IL, 61820, USA;
e-mail: amiller7@illinois.edu
Jacques Fournier, Las Muros, 09420 Rimont, France; e-mail: jacques.fournier@club-internet.fr
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
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Persoonia – Volume 36, 2016
Uwemyces elaeidis
455
Fungal Planet description sheets
Fungal Planet 468 – 4 July 2016
Uwemyces Hern.-Restr., G.A. Sarria & Crous, gen. nov.
Etymology. Named for Prof. Uwe Braun, who greatly contributed to our
knowledge of dematiaceous hyphomycetous fungi.
Classification — Mycosphaerellaceae, Capnodiales, Dothi
deomycetes.
Mycelium immersed and superficial, hyphae branched, septate,
hyaline and brown, smooth-walled. Conidiophores fasciculate,
simple, dark brown at the base and subhyaline at the apex.
Conidiogenous cells cylindrical, sympodial, polytretic, with dark
scars, terminal and intercalary, brown. Conidia straight or
curved, cylindrical to obclavate, pale brown to brown, apex subhyaline, verruculose-walled, with a thick, dark brown, truncate
scar at the base, septate. Sexual morph unknown.
Type species. Uwemyces elaeidis Hern.-Restr., G.A. Sarria & Crous.
MycoBank MB816986.
Uwemyces elaeidis (Steyaert) Hern.-Restr., G.A. Sarria & Crous, comb. nov.
Basionym. Cercospora elaeidis Steyaert, Bull. Soc. Roy. Bot. Belgique
80: 35. 1948; as ‘elaedis’.
≡ Pseudospiropes elaeidis (Steyaert) Deighton, Trans. Brit. Mycol. Soc.
85, 4: 739. 1985.
Mycelium immersed and superficial, hyphae branched, septate,
hyaline and brown, smooth-walled. Conidiophores fasciculate,
simple, dark brown at the base and subhyaline at the apex,
96.5–188 × 6–9 µm. Conidiogenous cells cylindrical, sympodial, with dark brown scars, terminal and intercalary, brown,
28 –70 × 5 – 9 µm. Conidia cylindrical to obclavate, straight
or curved, pale brown to brown, apex subhyaline, 2–3.5 µm
wide, wall verruculose, 82–133 × 6–8.5 µm, 4–8-septate, with
a thick, dark brown, truncate scar at the base, 2–3.5 µm wide.
Sexual morph unknown.
Culture characteristics — Colonies on OA, reaching 10 mm
diam after 4 wk at 25 °C in the dark. Convex, cottony, vinaceous
buff in the centre and velvety, olivaceous black towards the
periphery, margin fimbriate; reverse black. On MEA, reaching
7 mm diam after 4 wk at 25 °C in the dark. Convex with papillate surface, centre vinaceous buff, periphery olivaceous black,
margin lobed; reverse black.
Specimen examined. coloMbia, Barrancabermeja, CENIPALMA, on
leaves of Elaeis oleifera, May 2013, coll. G.A. Sarria (culture CPUwZC-01,
ITS sequence GenBank KX228299.1, LSU sequence GenBank KX228356.1,
rpb2 sequence GenBank KX228371.1, MycoBank MB816987).
Notes — The generic affinity of this species has been recently discussed by Braun et al. (2014). It was excluded from
Cercospora and it is not congeneric with Pseudospiropes where
it was tentatively placed (Steyaert 1948, Deighton 1985). Our
molecular result suggests that Uwemyces elaeidis is related to
members of Mycosphaerellaceae, and represents a different genus in this family. This species has wide distribution and seems
to be restricted to Elaeis guineensis, Arecaceae (Braun et al.
2014). Unfortunately, it was not possible to propose a formal
neotypification, since the geographical origin of the specimen
examined was not the same as described in the protologue
(Democratic Republic of the Congo, Kodoro).
ITS. Based on a megablast search of NCBIs GenBank nucleotide database, the closest hits using the ITS sequences are
Pseudocercospora cladrastidis (GenBank AB694922; Identities
= 463/508 (91 %), Gaps = 22/508 (4 %). GenBank AB694923;
Identities = 462/507 (91 %), Gaps = 20/507 (3 %). GenBank
AB694921; Identities = 462/507 (91 %), Gaps = 20/507 (3 %)),
Cercosporella dolichandrae (GenBank KJ869140; Identities =
496/546 (91 %), Gaps = 12/546 (2 %) and Pseudocercospora
ocimicola (GenBank GU214678; Identities = 497/548 (91 %),
Gaps = 10/548 (1 %)).
LSU. Based on a megablast search of NCBIs GenBank nucleotide database, the closest hits using the LSU sequences
are Mycosphaerella swartii (GenBank DQ923536; Identities =
828/860 (96 %), Gaps = 8/860 (0 %)), Mycosphaerella walk
eri (GenBank DQ267574; Identities = 828/860 (96 %), Gaps
= 8/860 (0 %)) and Acervuloseptoria ziziphicola (GenBank
KJ869221; Identities =796 /828 (96 %), Gaps = 8/828 (0 %)).
rpb2. Based on a megablast search of NCBIs GenBank nucleotide database, the closest hits using the rpb2 sequences are
Pseudocercospora norchiensis (GenBank JQ739865; Identities = 192/229 (84 %), Gaps = 4/229 (1 %), GenBank KF902320;
Identities = 200/239 (84 %), Gaps = 4/239 (1 %), GenBank
JX902017; Identities = 200/239 (84 %), Gaps = 4/239 (1 %))
and Pseudocercospora atromarginalis (GenBank JX902006;
Identities = 205 /245 (84 %), Gaps = 4/245 (1 %)).
Colour illustrations. Elaeis oleifera palm tree in CENIPALMA Colombia;
leaf spots, conidiophores, conidiogenous cells and conidia in natural substrate
and in culture. Scale bars = 10 µm.
Margarita Hernández-Restrepo & Pedro W. Crous, CBS-KNAW Fungal Biodiversity Centre, P.O. Box 85167, 3508 AD Utrecht, The Netherlands;
e-mail: m.hernandez@cbs.knaw.nl & p.crous@cbs.knaw.nl
Greicy Andrea Sarria, Corporación Centro de Investigación en Palma de Aceite (CENIPALMA), Colombia; e-mail: gsarria@cenipalma.org
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
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Persoonia – Volume 36, 2016
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