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ড. "ক. এম. "গালাম দ*গীর Dr. K. M. Golam Dastogeer িব.এসিস.এিজ. (অনাস3), এম.এস. উি5দ "রাগতT (বাক8িব), িপএইচিড (মারডক, অে=িলয়া) B.Sc.Ag.(Hons.), M.S.PPath (BAU), Ph.D (Murdoch, Australia) Assistant Professor সহকারী অধAাপক উি5দ "রাগতT িবভাগ বাংলােদশ ক8িষ িবFিবদAালয়, ময়মনিসংহ-২২২২ Department of Plant Pathology Bangladesh Agricultural University Mymensingh-2202 "ফান (বAািIগত): + ৮৮-০১৭৮৭-৪২৪-৭৮৮ Phone (Cell): +88-01787-424-788 "ফান (অিফস): +৮৮-০৯১-৬৪৫২৫ Phone (off.): +88-091-64525 Leaf spot of Groundnut This disease is also known as ‘tikka disease’. Two types of symptoms are produced and are known as: early leaf spot and late leaf spot. They look very similar and may need microscopic examination to differentiate them. They are often found together in the same crop and even on the same plant. There are slight differences in symptoms, explained below, but from a farmer’s point of view they are the same disease and should be treated as such. Early leaf spot Early leaf spot is caused by Cercospora arachidicola or Mycosphaerella arachidis The symptoms of early leaf spots are characterized by the appearance of large, roughly circular reddishbrown spots on the upper leaf surface. The spots are surrounded by a bright yellow halo with a dark brown/ brown red center. On the lower leaf surface, the spots are light brown and the yellow halo is indistinct or absent The lower surface of the spots is carbon black in colour. Some spots enlarged and coalesced to form dark brown to black, blighted patches. Fig. Late leaf spot of groundnut Description of the causal organisms Mycelium: Mycelium is composed of fine to coarse, colored and septate hyphae. Conidiophores Conidiophores are short, simple, unbranched, septate, arising from stromata or directly from vegetative hyphae, aggregated in fascicles (a cluster or bundle) of five to many, pale brown in color which are darker at the base. In case of early spot, conidiophores appear mostly on the upper surface of the leaf while in late spots: conidiophores appear mostly on lower surface of the leaf. Fig. Early leaf spot of groundnut Late leaf spot Late leaf spot is caused by Cercosporidium personatum or Mycosphaerella berkeleyi. The symptoms of late leaf spots are characterized by the appearance of comparatively small, circular, dark brown to black spots on the both sides of leaf (mostly on the lower leaf surface). Conidia of Cercospora arachidicola Conidia are light brown, filiform, mildly to much curved, have upto 12 septa, with a truncate base and sub-acute/bluntly rounded ends. Conidia of Cercosporidium personatum Conidia are cylindrical, obclavate, usually straight or slightly curved, pale brown, with a finely roughened wall that is rounded at the apex, usually 3-4 septa, base is shortly tapered with a conspicuous hilum. www.bau.edu.bd/profile/PPATH1013 www.researchgate.net/profile/Khondoker_M_G_Dastogeer scholar.google.com.au/citations?user=cX2Z0wUAAAAJ&hl=en http://orcid.org/0000-0003-4607-119X Management Options Different Structiures of the causal organism of leaf spot of groundnut. (A) Conidia of C. arachidicola (B) Conidia of C. personatum Scientific Classification Traditional Form-Phylum: Deuteromycotina Class: Hyphomycetes Order: Moniliales Family: Dematiaceae Genus: Cercospora/Cercosporidium Species: C. arachidicola/ C. personatum Grow resistant variety (such as BARI Chinabadam- 1, 2, 3) Where possible, there should be a distinct break in time between successive groundnut crops. Mix crops of groundnut with maize or sorghum. Use correct planting density (50 cm x 30 cm or 50 cm x 40 cm) As the diseases are largely soil-borne, rotation with other crops (such as cowpea and maize.) is very important Remove plant debris from the field after harvest, burned in situ, fed to animals, or deep-buried. Volunteer groundnut plants and 'ground-keepers' should be eradicated. Keep weeds under control because their heavy growth may encourage disease development through modification of the crop microclimate. Plough and harrow to cover crop residues with soil after harvest and to destroy fungus in the soil Remove heavily infected plants to prevent spread of the fungus Destroy diseased debris and avoid excessive irrigation Spray fungicides such as Propiconazole (such as Tilt 50EC) or Mancozeb (Dithane M 45) @ 0.2% at 12-15 days interval. When using a pesticide, always wear protective clothing and follow the instructions on the product label, such as dosage, timing of application, and preharvest interval. Current Phylum: Ascomycota Class: Dothideomycetes Subclass: Dothideomycetidae Order: Capnodiales Family: Mycosphaerellaceae Genus: Cercospora (Mycosphaerella) Cercosporidium (Mycosphaerella) Species: C. arachidicola (M. arachidis) C. personatum (M. berkeleyi) Page 02