CACTI
CACTI
BIOLOGY AND USES
Edited by Park S. Nobel
UNIVERSITY OF CALIFORNIA PRESS
Berkeley
Los Angeles
London
University of California Press
Berkeley and Los Angeles, California
University of California Press, Ltd.
London, England
© 2002 by the Regents of the University of California
Library of Congress Cataloging-in-Publication Data
Cacti: biology and uses / Park S. Nobel, editor.
p. cm.
Includes bibliographical references (p. ).
ISBN 0-520-23157-0 (cloth : alk. paper)
1. Cactus. 2. Cactus — Utilization. I. Nobel, Park S.
qk495.c11 c185 2002
583'.56 — dc21
2001005014
Manufactured in the United States of America
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The paper used in this publication meets the minimum requirements of
ANSI /NISO Z39.48 – 1992 (R 1997) (Permanence of Paper).
C O N T E N TS
List of Contributors . vii
Preface . ix
1. Evolution and Systematics
Robert S. Wallace and Arthur C. Gibson . 1
2. Shoot Anatomy and Morphology
Teresa Terrazas Salgado and James D. Mauseth . 23
3. Root Structure and Function
Joseph G. Dubrovsky and Gretchen B. North . 41
4. Environmental Biology
Park S. Nobel and Edward G. Bobich . 57
5. Reproductive Biology
Eulogio Pimienta-Barrios and Rafael F. del Castillo . 75
6. Population and Community Ecology
Alfonso Valiente-Banuet and Héctor Godínez-Alvarez . 91
7. Consumption of Platyopuntias by Wild Vertebrates
Eric Mellink and Mónica E. Riojas-López . 109
8. Biodiversity and Conservation
Thomas H. Boyle and Edward F. Anderson . 125
9. Mesoamerican Domestication and Diffusion
Alejandro Casas and Giuseppe Barbera . 143
10. Cactus Pear Fruit Production
Paolo Inglese, Filadelfio Basile, and Mario Schirra . 163
11. Fruits of Vine and Columnar Cacti
Avinoam Nerd, Noemi Tel-Zur, and Yosef Mizrahi . 185
12. Forage, Fodder, and Animal Nutrition
Ali Nefzaoui and Hichem Ben Salem . 199
13. Nopalitos, Mucilage, Fiber, and Cochineal
Carmen Sáenz-Hernández, Joel Corrales-Garcia,
and Gildardo Aquino-Pérez . 211
14. Insect Pests and Diseases
Helmuth G. Zimmermann and Giovanni Granata . 235
15. Breeding and Biotechnology
Brad Chapman, Candelario Mondragon Jacobo,
Ronald A. Bunch, and Andrew H. Paterson . 255
Index . 273
C O N T R I BU TO R S
Edward F. Anderson (Deceased), Desert Botanical
Garden, Phoenix, Arizona
Gildardo Aquino-Prez, Insituto de Recursos
Genéticos y Productividad, Montecillo, Mexico
Giuseppe Barbera, Istituto di Coltivazioni Arboree,
Università degli Studi di Palermo, Italy
Filadelfio Basile, Dipartimento Scienze EconomicoAgrarie ed Estimativ, Universita degli Studi di Catania,
Italy
Hichem Ben Salem, Institut National de la
Recherche Agronomique de Tunisie, Laboratoire
de Nutrition Animale, Ariana, Tunisia
Edward G. Bobich, Department of Organismic
Biology, Ecology and Evolution, University of
California, Los Angeles
Thomas H. Boyle, Department of Plant and Soil
Sciences, University of Massachusetts, Amherst
Ronald A. Bunch, D’Arrigo Bros. Co., Salinas,
California
Alejandro Casas, Departamento de Ecología de los
Recursos Naturales, Instituto de Ecología, Universidad
Nacional Autónoma de México, Morelia
Brad Chapman, Plant Genome Mapping Laboratory,
University of Georgia, Athens
Joel Corrales-Garca, Departamento de Ingeniería
Agroindustrial, Universidad Autónoma de Chapingo,
Mexico
Rafael F. del Castillo, Centro Interdisciplinario
de Investigacíon para el Desarrollo Integral Regional
Unidad Oaxaca, Mexico
Joseph G. Dubrovsky, Departamento de Biología
Molecular de Plantas, Instituto de Biotecnología,
Universidad Nacional Autónoma de México, Cuernavaca
Arthur C. Gibson, Department of Organismic
Biology, Ecology and Evolution, University of
California, Los Angeles
Hctor Godnez-Alvarez, Departamento de
Ecología Funcional y Aplicada, Instituto de Ecología,
Universidad Nacional Autónoma de México, Mexico
City
Giovanni Granata, Dipartimento di Scienze e
Technologie Fitosanitartie, Università degli Studi di
Catania, Italy
Paolo Inglese, Istituto di Coltivazioni Arboree,
Palermo, Italy
James D. Mauseth, Department of Integrative
Biology, University of Texas at Austin
Eric Mellink, Centro de Investigación Cientifica
y de Educación Superior de Ensenada, Mexico
vii
Yosef Mizrahi, Department of Life Sciences and
Institutes for Applied Research, Ben-Gurion University
of the Negev, Israel
Mnica E. Riojas-Lpez, Departamento de
Ecología, Centro Universitario de Ciencias Biológicas
y Agropecuarias, Universidad de Guadalajara, Mexico
Candelario Mondragon Jacobo, Programa de
Nopal y Frutales, Instituto Nacional de Investigaciones
Forestales y Agropecuarias, Queretaro, Mexico
Carmen Senz-Hernndez, Departamento de
Agroindustria y Enología, Facultad de Ciencias Agrarias
y Forestales, Universidad de Chile, Santiago
Ali Nefzaoui, Institut National de la Recherche
Agronomique de Tunisie, Laboratoire de Nutrition
Animale, Ariana, Tunisia
Mario Schirra, Instituto per la Fisologia della
Maturazione e della Conservazione del Frutto delle
Specie Arboree Mediterranee, Oristano, Italy
Avinoam Nerd, Institutes for Applied Research,
Ben-Gurion University of the Negev, Israel
Noemi Tel-Zur, Department of Life Sciences,
Ben-Gurion University of the Negev, Israel
Park S. Nobel, Department of Organismic Biology,
Ecology and Evolution, University of California, Los
Angeles
Teresa Terrazas Salgado, Programa de Botánica,
Colegio de Postgraduados, Montecillo, Mexico
Gretchen B. North, Department of Biology,
Occidental College, Los Angeles, California
Andrew H. Paterson, Plant Genome Mapping
Laboratory, University of Georgia, Athens
Eulogio Pimienta-Barrios, Departamento de
Ecología, Centro Universitario de Ciencias Biológicas
y Ambientales, Universidad de Guadalajara, Mexico
viii
Contributors
Alfonso Valiente-Banuet, Departamento de
Ecología Funcional y Aplicada, Instituto de Ecología,
Universidad Nacional Autónoma de México, Mexico
City
Robert S. Wallace, Department of Botany, Iowa
State University, Ames
Helmuth G. Zimmermann, Plant Protection
Research Institute, Agricultural Research Council,
Pretoria, South Africa
P R E FAC E
The Cactaceae, a family of approximately 1,600 species, is
native to the New World but is cultivated worldwide. In response to extreme habitats, cacti have evolved special physiological traits as well as distinctive appearances. The stem
morphology, spine properties, and often spectacular flowers
have caused hobbyists to collect and cultivate large numbers
of cacti. Both cactus form and function relate to nocturnal
stomatal opening and Crassulacean acid metabolism, which
lead to efficient use of limited soil water. Thus, cacti can
thrive in arid and semiarid environments, where they are
often important resources for both wildlife and humans.
Indeed, cacti have been consumed by humans for more
than 9,000 years. Currently, Opuntia ficus-indica is cultivated in over 20 countries for its fruit, and an even greater
land area is devoted to its cultivation for forage and fodder.
The fruits of other cactus species, known as pitahayas and
pitayas, and various other cactus products are appearing in
an increasing number of markets worldwide.
Due to the high water-use efficiency and other adaptations of cacti, biological and agronomic interest in them has
soared. From 1998 to 2000, more than 600 researchers published over 1,100 articles on cacti, including papers in proceedings of national and international meetings. Yet a current, synthetic, widely ranging reference is not available.
This book, which consists of a series of authoritative, up-todate, review chapters written by established experts as well
as new contributors, emphasizes both the biology of cacti
and their uses. Twelve authors are from Mexico, eleven from
the United States, five from Italy, three from Israel, two
from Tunisia, and one each from Chile and South Africa.
Most of the authors share my interests in basic research on
the Cactaceae. Nearly half of the authors, especially those
dealing with agronomic aspects, are involved with the
CactusNet sponsored by the Food and Agricultural Organization of the United Nations. Approximately 1,300 references are cited in the chapters, which not only indicate the
widespread interest in cacti but also should facilitate further
investigations. The intended audience ranges from ecologists and environmentalists to agriculturalists and consumers to cactus hobbyists and enthusiasts.
The point of departure is the evolution of the family in
the broad sense, paying particular attention to new molecular and genetic approaches (Chapter 1). People recognize cacti by their shoot morphology, which reflects various cellular characteristics (Chapter 2). The uptake of
water and nutrients from the soil by roots that sustains the
shoots has unique features as well (Chapter 3). Survival depends on adaptation to abiotic environmental conditions,
which cacti have done in special ways (Chapter 4). In addition to enduring harsh conditions, cacti must reproduce,
for which many strategies have evolved (Chapter 5). Biotic
factors are also crucial for the success of cacti in natural environments (Chapter 6). Because of their ecological success, cacti are important food resources for wild vertebrates
ix
(Chapter 7). The many unique characteristics of the
Cactaceae have attracted collectors and raised concerns
about issues of biodiversity and conservation (Chapter 8)
as well as led to their ancient usage and subsequent wide
diffusion by humans (Chapter 9). The most widespread
use occurs for fruits of platyopuntias, known as cactus
pears (Chapter 10). Also, fruits of vine-like and columnar
cacti are increasingly popular in many countries (Chapter
11). An even greater land area worldwide than is used for
cactus fruits is devoted to raising platyopuntias for forage
and fodder (Chapter 12). Besides such uses, cacti are also
important as a vegetable, as a dietary supplement, and as
the host for the red-dye-producing cochineal (Chapter 13).
Such uses, which are constrained by pests and diseases
(Chapter 14), are currently expanding via breeding and
biotechnology (Chapter 15).
Special thanks are due to those who helped in the realization of this book. Edward Bobich helped prepare the
line drawings and halftones for reproduction, and Erick
x
Preface
De la Barrera assisted with the many Spanish citations.
Marian McKenna Olivas competently did line editing, and
Alicia Materi meticulously typed the developmental and
line editing changes. Financial support for these steps was
provided by Sol Leshin, a man of integrity and generosity
with a profound interest in plants and their uses dating
back to his M.S. in soil science in 1938. Numerous suggestions on improving the arrangement and scientific content
were the result of a graduate course taught from the book
and attended by Edward Bobich, Erick De la Barrera, C. J.
Fotheringham, Catherine Kleier, and Alexandra Reich.
The dedication and important suggestions of these people
helped meld the contributions of a diverse group of authors into the final product, for which I am extremely
grateful.
Park S. Nobel
February 10, 2001
CHAPTER
›1‹
EVO LU T I O N A N D S Y S T E M AT I C S
Robert S. Wallace and Arthur C. Gibson
Introduction
Phylogenetic Placement of Cactaceae
Cactaceae, a Family of Order Caryophyllales
Classification of Cactaceae within Suborder Portulacineae
Cactaceae, a Monophyletic Family
Defining Subfamilies of Cactaceae
Transitions from Structural Analyses to Molecular Systematics
Molecular Systematics of Cactoideae
Identifying the Oldest Taxa
Epiphytic Cacti
Columnar Cactus Lineages
Cacteae and Notocacteae
Solving Classification Problems Using Molecular Techniques
Phylogenetic Studies of Subfamily Opuntioideae
New Insights into Cactus Evolution
Structural Properties
Revised Biogeographic Models Based on Molecular Studies
Concluding Remarks
Literature Cited
Introduction
The Cactaceae is one of the most popular, easily recognizable, and morphologically distinct families of plants, and
it includes approximately 1,600 species (Gibson and Nobel
1986; Barthlott and Hunt 1993). Cacti occur in the New
World from western and southern Canada (Speirs 1982)
to southern Patagonia in Chile and Argentina (Kiesling
1988), and the epiphytic genus Rhipsalis has dispersed naturally, undoubtedly by birds, to tropical Africa and Madagascar and across to Sri Lanka and southern India (Thorne
1973; Barthlott 1983). These usually spiny organisms (Fig.
1.1) are loved by plant fanciers for their diverse forms and
showy flowers. Nearly every introductory college biology
or ecology textbook contains at least one cactus photograph, used to illustrate plant adaptation to dry habitats.
Important commercial products are derived from cacti
(Nobel 1994, 1998). Cacti have also helped evolutionary biologists and ecologists understand CAM (Crassulacean
acid metabolism) and succulence (Gibson and Nobel
1986; Nobel 1988, 1991).Fgiunr1e.eahrere:
In some plant families, it is merely a matter of con-
1
Figure 1.1. The vegetative plant of Coryphantha bumamma (Ehrenberg) Brittton and Rose (tribe Cacteae), a low-growing spherical cactus from
Guerrero, Mexico.
venience to have correct names for plant species. In the
Cactaceae, however, there is not only a huge demand for
correct names and precise classification into genera, but
also a critical need for a phylogenetic classification because
there are many subjects, some of which are covered in this
book, that depend on having an accurate evolutionary reconstruction of cactus history.
Phylogenetic Placement of Cactaceae
Cactaceae, a Family of Order Caryophyllales
Family Cactaceae is assigned to order Caryophyllales,
which includes, among others, ice plants (Aizoaceae),
portulacas (Portulacaceae), carnations (Caryophyllaceae),
bougainvilleas (Nyctaginaceae), pokeweeds (Phytolaccaceae), amaranths (Amaranthaceae), and saltbushes
(Chenopodiaceae). The taxonomic history of classifying
Cactaceae within this order has been adequately reviewed
(Cronquist and Thorne 1994), and there is universal acceptance that cacti are core members of Caryophyllales.
Phylogenetic placement within the Caryophyllales is
undisputed, because cacti and other families within the
2
Wallace and Gibson
order share derived characters, i.e., synapomorphies, that
do not occur in any other angiospermous order. One structural synapomorphy, and the first recognized feature for relating these families, is that the seed contains a strongly
curved, peripheral embryo around a central nutritive
perisperm, not endosperm. From that observation arose
the ordinal name Centrospermae (Eichler 1878). A chemical synapomorphy is the occurrence of betalains, a class of
nitrogenous pigments derived from tyrosine (Mabry 1964;
Clements et al. 1994). The Cactaceae and closely related
families form a proteinaceous plastid inclusion (designated as type P3cf ) during the ontogeny of sieve-tube members (Behnke 1976a,b, 1994). Congruence of the three
mentioned unlinked and unique synapomorphic characters in these same families, not in others, formed a solid
case for recognizing this monophyletic clade.
Order Caryophyllales, which was established by analyzing certain types of structural and chemical data, was
tested with a new data set using chloroplast DNA (cpDNA)
restriction site mutations, and was confirmed by the loss
of the rpl2 intron in the common ancestor of the order
(Downie and Palmer 1994). Indeed, investigators use
whatever data are available at the time to formulate an initial hypothesis, and later test the model using an indisputable data set of a totally different nature that provides
resolution. Yet there are still some unresolved issues concerning the composition of Caryophyllales and whether
other families, shown by molecular studies to share closest
DNA affinities to Caryophyllales, should be classified
within the order (Angiosperm Phylogeny Group 1998).
Among these are the insectivorous sundews (Droseraceae)
and pitcher plants of Nepenthaceae. It is unclear at this
time whether molecular data will require these nontraditional members to be classified within the order or instead
as allies in one or more separate orders. Regardless of that
outcome, placement of family Cactaceae is unaffected for
the time being.
Classification of Cactaceae within Suborder Portulacineae
Phylogenetic relationships of the Cactaceae within the
Caryophyllales have been much more difficult to determine. Investigators have been interested in determining to
which of the betalain-containing families Cactaceae is phylogenetically most closely related. Traditional comparative
and developmental evidence favored the Aizoaceae (Turner
1973; Rodman et al. 1984) or Phytolaccaceae (Buxbaum
1953; Cronquist 1981), emphasizing floral features. More recent analyses claimed that the Cactaceae has most recent
ancestry with the Portulacaceae (Thorne 1983; Gibson and
Nobel 1986; Hershkovitz 1991; Gibson 1994), within what
became called suborder Portulacineae Thorne (Cronquist
and Thorne 1994), which included Cactaceae, Portulacaceae, Didiereaceae, and Basellaceae.
New data sets from gene sequence experiments tested
the model and strongly supported Portulacineae as a
monophyletic taxon that includes Cactaceae (Manhart and
Rettig 1994). Cactaceae and certain Portulacaceae are sister taxa sharing a 500 base-pair (bp) deletion in the
Rubisco gene rbcL (Rettig et al. 1992; Downie and Palmer
1994). Using a 1,100 bp sequence of open reading frame in
cpDNA, the largest gene in the chloroplast genome,
Downie et al. (1997) concluded again that Pereskia (Cactaceae) belongs in the portulacaceous cohort. With internal
transcribed spacer sequences of cpDNA, Hershkovitz and
Zimmer (1997) obtained results that placed the primitive
leaf-bearing cacti phylogenetically nested within the
Portulacaceae, and the Cactaceae was identified as the sister taxon of a clade that includes species of Talinum. In a
more intensive cpDNA analysis of the portulacaceous cohort, using gene sequence data of ndhF, a recent study has
shown that the Cactaceae is indeed nested within the
Portulacaceae sensu lato and is most closely related to
Talinum and Portulaca (Fig. 1.2; Appleqvist and Wallace
2001). In future systematic studies of the family, these sequence data will play an important role in redefining the
family Portulacaceae, as well as the evolutionarily distinct
groups it now contains, and how the evolutionary components of this diverse clade need to be circumscribed.
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Cactaceae, a Monophyletic Family
Even casual students of cacti can recognize the repetitive
vegetative design within this plant family (Gibson and
Nobel 1986). Typically, a cactus possesses a perennial photosynthetic succulent stem, bearing leaf spines produced on
modified axillary buds, termed areoles, but lacking broad
green leaves. The colorful flower of the typical cactus has
many separate perianth parts, numerous stamens, and an inferior ovary with many ovules and parietal placentation. The
fruit is a many-seeded berry, often juicy but in some taxa becoming dry or splitting open at maturity. There are, of
course, exceptional forms: (1) spineless plants (e.g., certain
epiphytes such as Disocactus and Epiphyllum and small cacti
such as Lophophora and Ariocarpus); (2) geophytes with annual above-ground shoots (e.g., Pterocactus kuntzei, Opuntia
chaffeyi, and Peniocereus striatus); (3) primitive cacti that
have relatively broad, dorsiventrally flattened leaves (e.g.,
Pereskia spp. and Pereskiopsis porteri); (4) plants that have
relatively small flowers with fewer parts (e.g., small-flowered
species of Rhipsalis, Pseudorhipsalis, and Uebelmannia spp.);
and (5) superior ovaries with axile placentation (e.g., Pereskia
sacharosa). None of these exceptions is troubling, because all
are well-accepted members of the family and understood as
representing either primitive or highly reduced, apomorphic
(derived) states of cactus features.
The morphological distinctiveness and monophyly of
family Cactaceae have been further supported conclusively with molecular data. There has occurred a 6 kb inversion
in the large single copy region of the plastid genome (relative to the consensus land plant gene order seen in
Nicotiana tabacum; Downie and Palmer 1993) that involves
the genes atpE, atpB, and rbcL. This cpDNA inversion has
been found in all cacti sampled, so this is an excellent
molecular synapomorphy for defining Cactaceae (Wallace
1995; Wallace and Forquer 1995; Wallace and Cota 1996;
Cota and Wallace 1996, 1997). Remarkably, an identical inversion of the same cpDNA region occurs independently
in another caryophyllalean lineage, the Chenopodiaceae
(Downie and Palmer 1993). Nonetheless, because cacti
consistently exhibit this 6 kb inversion, molecular systematists infer that Cactaceae are monophyletic, i.e., traceable
back to a single ancestral population in which the inversion
appeared and then became genetically fixed. What remains
Evolution and Systematics
3
Amaranthus palmeri
A. quitensis
Mollugo verticillata
AMARANTHACEAE
MOLLUGINACEAE
Allionia violacea
Mirabilis jalapa
NYCTAGINACEAE
Bougainvillea sp.
Phytolacca acinosa
PHYTOLACCACEAE
Aptenia cordifolia
Tetragonia tetragonioides
AIZOACEAE
Talinum paniculatum
T. angustissimum
T. caffrum
T. triangulare
Talinella pachypoda
Anacampseros retusa
Grahamia bracteata
Talinopsis frutescens
Portulaca grandiflora
P. mundula
P. molokiniensis
P. oleracea
Maihuenia poeppigii
Pereskia aculeata
CACTACEAE
Quiabentia verticillata
Montia perfoliata
Claytonia virginica
Montia diffusa
M. parvifolia
Lewisia pygmaea
Calandrinia volubilis
C. ciliata var. menziesii
C. compressa
Montiopsis umbellata
M. berteroana
M. cumingii
Cistanthe grandiflora
C. mucronulata
C. guadalupensis
Calyptridium umbellatum
Talinum mengesii
Alluaudia humbertii
Didierea trollii
DIDIEREACEAE
Calyptrotheca somalensis
Ceraria fruticulosa
Portulacaria afra
Basella alba
Ullucus tuberosus
BASELLACEAE
Figure 1.2. Strict consensus tree of equally parsimonious trees from analysis of the ndhF gene sequence for the portulacaceous alliance,
which includes Cactaceae, Portulacaceae, Didiereaceae, and Basellaceae (after Appleqvist and Wallace 2000).
unresolved is whether investigators eventually will recognize more than one family of the cacti for this evolutionary branch.
Defining Subfamilies of Cactaceae
All recent familial classifications of Cactaceae have recognized three major clades, most commonly classified as subfamilies: Pereskioideae, Opuntioideae, and Cactoideae
(Hunt and Taylor 1986, 1990; Gibson and Nobel 1986;
Barthlott 1988; Barthlott and Hunt 1993). Each subfamily
is distinguished by structural criteria, for which there are
relatively clear discontinuities among these three clades.
Subfamily Pereskioideae has been defined essentially as
the pool of extant cacti with the primitive vegetative and
reproductive features (Buxbaum 1950; Boke 1954; Bailey
1960; Gibson 1976; Gibson and Nobel 1986). As traditionally defined, this subfamily has no known structural
synapomorphy (Barthlott and Hunt 1993). Two genera
have been assigned to this subfamily: Pereskia (16 spp.;
Leuenberger 1986) and the Patagonian Maihuenia (2 spp.;
Gibson 1977b; Leuenberger 1997). The broad-leaved
shrubs and trees of Pereskia and small-leaved, moundforming plants of Maihuenia have totally different external
vegetative morphology and anatomy but share some plesiomorphic (primitive) reproductive features (Buxbaum
1953). Vegetative morphology of Maihuenia grades into
low-growth forms of Opuntioideae. In fact, both species of
Maihuenia were originally described as species of Opuntia
(Leuenberger 1997).
Subfamily Opuntioideae is the most easily defined by
its structural synapomorphies: (1) areoles have glochids,
i.e., very short and fine deciduous leaf spines that have
retrorse barbs and are easily dislodged; (2) every cell comprising the outer cortical layer of the stem possesses a large
druse, i.e., an aggregate crystal of calcium oxalate (Bailey
1964; Gibson and Nobel 1986); (3) pollen grains are polyporate and possess peculiar microscopic exine features
(Leuenberger 1976); (4) the seed is surrounded by a funicular envelope, often described as being an aril; and (5) special tracheids occurring in secondary xylem (wide-band
tracheids of Mauseth 1993a, 1995; vascular tracheids of
Bailey 1964, 1966 and Gibson 1977a, 1978) possess only
annular secondary thickenings (Gibson and Nobel 1986).
Other distinguishing features could be listed but are not
true synapomorphies, i.e., derived character states within
the family.
Subfamily Cactoideae is less easily delimited by synapomorphies. In fact, probably only one general form applies to all genera: namely, the stem is succulent and pos-
sesses a minute, often microscopic, upper leaf (Oberblatt)
subtending each areole (Boke 1944). This contrasts with
Opuntioideae, in which the leaf is usually small, terete,
succulent, and easily discernible to the unaided eye. In
most species of the subfamily, stems of Cactoideae have
ribs (tubercles and areoles are arranged in a vertical series),
but this cannot qualify as a synapomorphy and would ignore the presence of stem ribs of certain Opuntioideae, especially corynopuntias (Grusonia). Nonetheless, among extant cacti, there are no apparent morphological stages
linking the leafy, nonsucculent, aerole-bearing shoots of
Pereskia to any of the suggested primitive ribbed forms of
Cactoideae. Other features that clearly differentiate between leafy pereskias and plesiomorphic Cactoideae, such
as an outer stem cortex consisting of multiseriate hypodermis, are also found in Opuntioideae.
New evidence to evaluate the commonly used subfamilial classification of Cactaceae comes from analyses of
cpDNA structural arrangements of the chloroplast genome
adjacent to the region of the rbcL gene and comparative sequencing of a number of plastid coding and noncoding sequences. Opuntioideae are clearly demarcated molecularly by the deletion of the gene accD (ORF 512) in the plastid
genome (Wallace 1995). All Cactoideae examined to date
have a different deletion at the 5' end of the accD region
and have lost the intron to the plastid gene rpoC1, a deletion of approximately 740 bp, which supports a common
ancestry for all members of this subfamily (Wallace 1995;
Wallace and Cota 1996). The clades defined by these structural rearrangements are further supported by phylogenies
determined from comparative sequencing.
Unfortunately, a unique genetic synapomorphy has
not yet been discovered for subfamily Pereskioideae, as previously circumscribed, but Pereskia and Maihuenia are
themselves divergent because they have not been found to
share restriction site changes, although many occur
uniquely as synapomorphies for each genus (Wallace 1995).
In fact, nucleotide sequencing data now demonstrate that
Pereskia and Maihuenia are as divergent from one another
as either is from Opuntioideae and Cactoideae.
Wallace (2002) used nucleotide sequence data as
justification to propose recognizing a fourth subfamily,
Maihuenioideae. When recognized as a separate subfamily, Maihuenioideae have distinctive structural synapomorphies, including curious anatomical features within leaves
not known to occur elsewhere in Cactaceae (Gibson
1977b; Leuenberger 1997). Wood features of Maihuenia are
also diagnostic to a specialist (Gibson 1977b), although all
the cell types found in Maihuenia, including the special
Evolution and Systematics
5
spindle-shaped tracheids with helical secondary thickenings, are also observed within other members of Cactoideae that have small growth forms (Gibson 1973; Gibson
and Nobel 1986; Mauseth 1995; Mauseth et al. 1995;
Mauseth and Plemons 1995).
The proposal by Wallace to recognize subfamily
Maihuenioideae was discussed openly for five years in deliberations and correspondence with Cactaceae specialists
of the International Organization for Succulent Plant
Study (IOS). The Cactaceae Working Party of the IOS
concentrated its efforts on clarifying infrafamilial relationships among species and genera and stabilizing nomenclature for the cactus family, in order to make informed decisions about revising its classification. This procedure, not
protected by the current international code of nomenclature, should become an accepted practice of the systematic community, instead of using preliminary publications to
justify scientific decisions. It may also become a standard
practice in the future to include molecular systematic studies or cladistic analyses of morphological or molecular data
as part of publishing a new plant species. In this regard, full
subfamilial diagnoses can be found for the Opuntioideae
and Cactoideae in Barthlott and Hunt (1993), for the
Maihuenioideae in Wallace (2002, after Leuenberger 1997),
and for the Pereskioideae, based on the diagnosis of
Pereskia in Leuenberger (1986).
Transitions from Structural Analyses to
Molecular Systematics
The 250-year history of cactus taxonomy and systematics,
as in all plant families, was dominated by the use of structural characters to assign species to genera. Unfortunately,
examples of evolutionary convergence and parallelism in
cactus structure are commonly observed (Table 1.1). These
include reversals in character states and neoteny, i.e., reversals to juvenile features. Losses of distinguishing taxonspecific features are certainly commonplace in this family,
in which plant habit, stem morphology, stem anatomy, and
flower characters have been targets of natural selection
(Buxbaum 1950, 1953; Gibson 1973; Gibson and Nobel
1986; Barthlott and Hunt 1993; Cornejo and Simpson
1997). What now worries cactus systematists are the unrecognized cases of parallel evolution still hidden among
the genera, where a feature has been relied on as being conservative but now is discovered not to be. Experts of a
group can sharply disagree on assigning a species to one
genus or another based on one individual emphasizing
seed characters, one flowers, and another areoles or internal anatomy. One of these characters—or none—may hold
the key to its real phylogeny, but which one?Tabn1e.leahrere:
6
Wallace and Gibson
Needed is a technique that is independent of structure,
where cases of parallelism and convergence can be clearly
recognized so that each species can be inserted into its
proper phylogenetic lineage. Application of molecular
systematic techniques to address these issues provides a
fresh look at old problems. The goal of modern plant systematics is to obtain, for each family, an entirely new and
potentially unbiased data set in which to test all presumed
classifications.
Molecular Systematics of Cactoideae
As of January 1, 2000, sequences for several plastid DNA
regions (rbcL, rpl16 intron, trnL-F intergenic spacer, ndhF)
for representative taxa within the Cactaceae have been
completed at Iowa State University (R. S. Wallace and
coworkers) and form the framework for phylogenetic comparisons of the various evolutionarily related groups within the family. Genomic DNA samples have been isolated
from photosynthetic stems (and leaves, when available)
representing all key species groups, including currently recognized genera, infrageneric taxa, and morphologically
anomalous species for which assignment to a genus has
been problematic. From the relatively small sample studied,
many systematic tangles are becoming unraveled each
time new groups are carefully sampled and analyzed. Even
so, Cactaceae must be more thoroughly subsampled, and
the task of processing hundreds of species is time consuming. Fortunately, molecular studies are no longer as costly
as they were a decade ago, due to advances in sequencing
technology. As the various evolutionary groups within the
Cactaceae are sampled more intensively, more robust phylogenies will emerge to provide a more certain assessment
of relationships within and among the subfamilies, tribes,
and genera that constitute the family.
Results from future studies of molecular variation
likely will be, as they have already been, very illuminating
in Cactaceae. New data can also be somewhat disturbing
in cases where it is learned how incorrect some previous
taxonomic placements were. These earlier classifications
mislead cactus systematists in attempts at classifying the
family and establishing scenarios for its evolutionary
changes. Findings from molecular studies have shown how
difficult it is to estimate affinities among cacti by using
only external or internal structural features. In practice, a
combination of molecular and morphological data will
serve to provide the best estimate of phylogeny within the
Cactaceae and will assist taxonomists in producing a
classification that incorporates evolutionary relationships
in its hierarchies, while establishing a usable and practical
classification.
TA B L E 1 . 1
Examples of parallel and convergent evolution of features within Cactaceae, using examples from North and South America
Taxon
Feature
Growth habit and wood anatomy
Creeping (procumbent) columnar
Living rocks
Massive barrel
Cylindrical barrel
Two-ribbed epiphyte
Resupinate epiphyte
Lateral cephalium
Epidermal papillae on green stem
Tubular red, hummingbirdpollinated flowers
Shrubs
Epiphytes
Hummingbird flowers with
red to brown pollen
Hawkmoth flowers, white, nocturnal
with long tube
Very small flowers
More than one flower per areole
Dark, glandular areolar trichomes
Hydrochorous (floating) seeds with
large hilum cup
Small seeds with large arillate strophiole
Mescaline
Large calcium oxalate druses in
outer cortex of stem
North America
South America
Stenocereus eruca
Ariocarpus fissuratus
Lophophora williamsii
Echinocactus ingens
Ferocactus wislizenii
Astrophytum myriostigma
Disocactus biformis
Selenicereus testudo
Cephalocereus senilis
Peniocereus marianus
Opuntia pilifera
Echinopsis coquimbanus
Neoporteria glabrescens
Oreocereus madisorianus
Eriosyce ceratistes
Denmoza rhodacantha
Copiapoa cinerea
Rhipsalis rhombea
Pseudorhipsalis amazonicus
Espostoa lanata
Pterocactus kuntzei
Tephrocactus auriculatus
Stenocereus alamosensis
Disocactus macdougallii
Cleistocactus strausii
Schlumbergera truncata
Echinocereus triglochidiatus
Mammillaria poselgeri
Cleistocactus brookei
Denmoza rhodacantha
Epiphyllum phyllanthus
Pseudorhipsalis spp.
Myrtillocactus cochal
Stenocereus thurberi
Selenicereus wittii
Rhipsalis spp.
Pseudorhipsalis amazonicus
Pilosocereus aurisetum
Astrophytum capricorne
Strombocactus disciformis
Lophophora williamsii
Stenocereus eruca
Frailea phenodisca
Blossfeldia liliputana
Echinopsis pachenoi
Opuntia basilaris
Aztekium ritteri
Monvillea spegazzini
References: Buxbaum (1950, 1955); Gibson (1973, 1988a,b); Rowley (1976); Bregman (1988, 1992); Rose and Barthlott
(1994); Zappi (1994); Barthlott and Porembski (1996); Porembski (1996); Barthlott et al. (1997).
Identifying the Oldest Taxa
When doing any type of contemporary phylogenetic analysis, the researcher must include at least one species that has
the presumed primitive features of the group being studied.
For Cactaceae as a whole, this has been easy because the leafbearing species of Pereskia and Maihuenia are undisputed
choices, and they are then assumed to have retained important plesiomorphic morphological or sequence characters for
phylogenetic analyses. For Opuntioideae also, the choice is
obvious with such leafy forms in the genera Pereskiopsis,
Quiabentia, or Austrocylindropuntia. However, for subfamily Cactoideae and each of its tribes, making an a priori
choice of taxa to best represent the primitive species has been
a field of great speculation and, until now, selecting the
primitive taxon has been a subjective process. Often, species
possessing primitive features are not the ones widely cultivated or readily available; these groups typically inhabit inaccessible localities or sites where collection is not frequent
and are usually incompletely described.
Evolution and Systematics
7
Buxbaum (1950) proposed that the primitive cereoid
cactus would logically be one that had a woody form like a
typical dicotyledon and relatively few ribs, e.g., in certain species of Leptocereus. Later, the tribe Leptocereeae
(Buxbaum 1958) was often used as a taxonomic category to
include cereoids having primitive vegetative and reproductive features. Out of that assemblage has emerged
Calymmanthium substerile Ritter from northern Peru,
which so far has served admirably as the outgroup for all
phylogenetic analyses of cpDNA variation in subfamily
Cactoideae (Fig. 1.3). In every molecular systematic study
conducted on subfamily Cactoideae, Calymmanthium was
found to be the most basal lineage in this group.Fgiur3en1.eahrere:
Calymmanthium is a poorly known columnar monotype. The few cultivated specimens exhibit juvenile shoots
with basitonic branching, whereas, in nature, this species
can achieve a height of 8 m (Backeberg 1976). Its solitary
flower develops in a bizarre way, in that the lower portion
is somewhat like a vegetative shoot with long, green scales,
whereas the upper portion is more like the typical cereoid
flower (Backeberg 1976). A liquid-preserved specimen of
C. substerile collected in the wild by Paul Hutchison
(3567, with J. K. Wright, January 1964; UCB jar 1000) is
stored at the University of California, Berkeley, herbarium.
This specimen has seven ribs, whereas juvenile shoots tend
to have only three or four (Backeberg 1962, 1976). This
species has simple stem anatomy, with an unremarkable
epidermis, a uniseriate to biseriate collenchymatous hypodermis with relatively thin walls, and no mucilage cells in
either cortex or pith.
When compared with other columnar cacti using molecular data, Calymmanthium lacks many of the synapomorphic nucleotide substitutions seen in the other tribal
groups. Based on the plastid DNA sequences studied to
date, it does not ally with either tribe Leptocereeae or
Browningieae, where it has been placed in previous taxonomic treatments, nor does it fall within the clade of the
predominantly South American columnar cacti of tribes
Cereeae or Trichocereeae. Indeed, C. substerile may be
the only remaining representative of a cactus lineage that
most closely represents the ancestral form of subfamily
Cactoideae.
There may be other, yet unstudied species that are also
plesiomorphic, relative to the majority of cacti in the subfamily, and would join C. substerile as “primitive outlier”
taxa. Other cacti showing little morphological differentiation from Calymmanthium are often considered “primitive” in the tribes to which they are associated (e.g.,
Corryocactus [including Erdisia], Lepismium [including
Pfeiffera and Lymanbensonia], and Leptocereus). Future
8
Wallace and Gibson
molecular studies will continue to elucidate the positions
of the most primitive members of the Cactoideae and will
add more systematic information to evaluate the position
of Calymmanthium and its placement as the basal lineage
of the subfamily.
Epiphytic Cacti
Nearly 130 epiphytic species of Cactaceae are found in the
neotropical forests and woodlands. Disocactus (including
Nopalxochia), Pseudorhipsalis, Epiphyllum, Rhipsalis, Hatiora,
and Schlumbergera are genera mainly of holoepiphytes, i.e.,
true epiphytes and epiliths that do not root in soil. Hylocereus (including Wilmattea) and Selenicereus include numerous species that are facultative epiphytes or secondary
hemiepiphytes, initially rooting in soil, and later becoming
fully epiphytic.
Epiphytic cacti arose from ribbed, terrestrial columnar
cacti. This was an obvious conclusion by early students and
collectors of cacti, and no one has ever suggested the reverse, because epiphytes are too highly specialized to have
given rise to the larger terrestrial cacti. Several major shifts
in structure from terrestrial to epiphytic life have been
hypothesized:
1. Epiphytes easily form adventitious roots along
the stem and use these roots to anchor themselves
to bark or rocks, as well as to absorb water and
minerals. Many cacti have the ability to form adventitious roots from stem tissues, but holoepiphytes
and hemiepiphytes do so while the stems are still
attached to the host plant.
2. Stems of many cactus holoepiphytes are broad
and leaflike, possessing a high surface-to-volume
ratio (Sajeva and Mauseth 1991). The ribs of holoepiphytes are thinner than ribs of terrestrial cacti,
not providing enough bulk to support an upright
plant and requiring the plant to live in wetter habitats because the stem does not store much water for
periods of drought. Holoepiphytes with very thin,
two-ribbed stems often do not possess a collenchymatous hypodermis (e.g., in Schlumbergera,
Disocactus, and Epiphyllum), whereas multiribbed
columnar stems always form this support tissue
(Gibson and Horak 1978).
3. Wood development is scanty, and the woody cylinder is very narrow, yielding a very thin and nonsucculent pith. Therefore, this wood is not used to support the plant, and the pith is not designed to store
water for dry seasons.
Ariocarpus
Mammillaria
CACTEAE
Echinocactus
Ferocactus
Armatocereus
Leptocereus
LEPTOCEREEAE
Bergerocactus
Carnegiea
Echinocereus
Escontria
PACHYCEREEAE
Polaskia
Stenocereus
Corryocactus
Arrojadoa
Gymnocalycium
Cactoideae
Browningia
Neoraimondia
Cereus
Cleistocactus
Espostoa
BCT CLADE
Harrisia
Oreocereus
Trichocereus
Discocactus
Stetsonia
Uebelmannia
Calymmanthium
Copiapoa
Notocactus
NOTOCACTEAE
Lepismium
Rhipsalis
Hatiora
RHIPSALIDAE
Schlumbergera
Epiphyllum
Nopalxochia
Maihuenioideae
HYLOCEREEAE
Hylocereus
Maihuenia
Opuntia phaeacantha
CACTACEAE
Tacinga
O. spinosior
Opuntioideae
Pereskiopsis
Quiabentia
Pterocactus
O. subulata
Pereskioideae
Pereskia aculata
P. grandifolia
Didiereaceae / Basellaceae
Portulacaceae
Alluaudia
Basella
Portulaca
Figure 1.3. Strict consensus tree of 22,400 equally parsimonious trees from analysis of the rbcL gene for the family Cactaceae. A total of
1,434 bp of sequence was used for comparisons. Some important nodes in this tree are still unresolved.
4. Spination on stems of cactus epiphytes, especially
on adult shoots, has been highly reduced or totally
eliminated. One might expect that these cacti lack
spines because hanging plants are not easily eaten
by mammals, but the most likely explanation is that
spines have been lost because they block sunlight
from reaching the photosynthetic tissues of the stem
(Gibson and Nobel 1986).
Cactus epiphytes are classified within two different
tribes, the primarily South American Rhipsalideae and the
primarily North American Hylocereeae, implying that
within Cactoideae epiphytism evolved independently at
least twice from terrestrial, ribbed columnar cacti, i.e., on
each of the continents (Gibson and Nobel 1986; Barthlott
1987). The speculation has been that Rhipsalideae evolved
from ancestors like Corryocactus (Barthlott 1988) in western South America, passing through transitional forms resembling Lepismium enroute to Rhipsalis, Schlumbergera,
and Hatiora, which inhabit the major center of diversity
for this tribe in Brazil. In North America, especially
Central America and the West Indies, shrubby species of
Hylocereeae, with arching stems and scandent growth
habits, would have been the ancestors of climbing
hemiepiphytes, e.g., Hylocereus and Selenicereus, as well as
the highly specialized two-ribbed, spineless holoepiphytes
of that tribe.
Molecular techniques have led to an important revelation. The tribes with epiphytes likely represent two of the
basal (i.e., the earliest divergent) lineages of subfamily
Cactoideae. Based on cladistic analysis of the chloroplastencoded gene rbcL, hylocereoid epiphytes of Disocactus
(subgenus Aporocactus), Epiphyllum, and Hylocereus, as well
as hemiepiphytes of Selenicereus, appear to have diverged as
a distinct lineage before, for example, Leptocereus and
Acanthocereus (Wallace 1995; Cota and Wallace 1996), and
prior to the divergence of most columnar and barrel cactus
lineages.
Early divergence of epiphytic groups from the columnar and barrel forms suggests that there was a rapid evolutionary radiation that occurred within subfamily Cactoideae. The hypothesized rapid radiation is likely the
reason for the lack of resolution (common occurrence of
polytomy) among the major tribal lineages of subfamily
Cactoideae. Until further studies of molecular variation are
complete—using additional DNA markers and more intensive sampling — the true branching order of the Cactoideae phylogenetic tree will remain unresolved and in a
“polytomy” state.
10
Wallace and Gibson
Columnar Cactus Lineages
Columnar cacti are presumably derived from a Calymmanthium-like ancestor that retained the upright, ribbed
habit. Many columnar cacti are capable of supporting massive stems with their combined rib, parenchymal, and vascular structures (Cornejo and Simpson 1997). Molecular
evidence currently suggests that there are two primary
clades of columnar cacti that arose from the South
American ancestral populations, each having inferred
common ancestries (Fig. 1.3). The first clade comprises
three former tribes that share a 300 bp deletion in Domain
IV of the plastid rpl16 intron, strongly suggesting a common ancestry based on this unique loss of DNA. Members
of the tribes Browningieae, Cereeae, and Trichocereeae all
share this DNA deletion (R. S. Wallace, unpublished observations). Acknowledging here the limited molecular
phylogenetic resolution found within this group of cacti to
date, the cohort of genera found with this 300 bp deletion
have been designated the “BCT” clade until more data are
found to resolve the actual intertribal and intergeneric relationships. The members of the BCT clade show tremendous diversity in growth habit, size, and habitat preferences, and this clade is exemplary in its levels of floral
morphological variation and suites of pollination types, including insect, bat, hawkmoth, and hummingbird syndromes. Interestingly, Buxbaum (1958) proposed that these
groups are related to one another and constituted one
major radiation in South American cacti. Based on the
scaly nature of the perianth in members of tribe Browningieae, members of Cereeae and Trichocereeae are assumed to be more recently derived than those of Browningieae. This assumption needs to be checked with
additional study and accompanying phylogenetic analysis.
Phylogeny of the North American columnar cacti is
somewhat better understood (Gibson and Horak 1978;
Gibson 1982; Gibson et al. 1986). Molecular data currently suggest that the two major lineages (tribes Leptocereeae
and Pachycereeae) arose from a Corryocactus-like transitional form (derived from the original Calymmanthiumlike ancestor in the northwestern Andes), and subsequently they radiated northward into North America
within two geographic zones. In Central America and the
Caribbean, Leptocereeae arose (Leptocereus, Acanthocereus,
and Dendrocereus), achieving maximal diversity in the
Greater Antilles, which formerly formed the backbone of
Central America (Gibson and Nobel 1986). The phylogenetic sister taxon to the Leptocereeae is tribe Pachycereeae,
identified as having two distinct evolutionary components
within it that are recognized taxonomically at the subtribe
level (Pachycereinae and Stenocereinae of Gibson and
Horak 1978; Gibson 1982; Cota and Wallace 1997). Numerous Pachycereeae and Leptocereeae may be characterized as having primarily bat pollination, although insect
and hummingbird pollination are found in some taxa.
Certain arborescent Pachycereeae form extensive woodlands in semiarid habitats throughout Mexico and other
places and provide an excellent example of ecological parallelisms for the extensive woodlands of Cereus, Echinopsis
(i.e., the Trichocerei), Browningia, and Armatocereus found
in similar habitats of South America.
Cacteae and Notocacteae
Systematic studies of the tribe Cacteae have begun to elucidate the complex intergeneric relationships in this, the
most speciose tribe of Cactoideae (Cota and Wallace 1997;
Butterworth and Wallace 1999; Butterworth et al. 2002).
Preliminary results reinforce the traditional hypothesis,
e.g., that of Buxbaum (1950) or Barthlott (1988), that the
ancestor of Cacteae probably was ribbed, and that the most
highly derived taxa often have tubercular stem structures,
as seen in Coryphantha and Mammillaria. This observation
is not surprising per se, because one expects the barrel cacti
with ribs to be derived from columnar cacti with ribs, and
the barrel cacti of Echinocactus and Ferocactus have often
been depicted as the basal taxa of the Cacteae. However, a
number of interesting revelations about certain genera and
their relationships are emerging from the molecular data
that directly address questions of generic circumscription
and monophyly. For example, as currently circumscribed,
the genera Ferocactus and Echinocactus are paraphyletic or
polyphyletic, and these species require further study to resolve the relationships as elucidated by morphological and
molecular characters. One particularly surprising discovery
originating from molecular studies is that the highly specialized plants of Aztekium, together with Geohintonia,
represent the most primitive living lineages of Cacteae.
This is an example where modern plants may manifest
highly specialized features, but they may still be considered
basal lineages when phylogenetic analyses of appropriate
data are conducted.
Mammillaria, the largest genus of the Cactoideae with
about 200 species, as currently treated, is monophyletic.
The peculiar species Oehmea beneckei and Mammilloydia
candida are clearly nested within Mammillaria and should
not, therefore, be recognized as segregate genera. A close
relationship between hummingbird-pollinated Cochemiea
and Mammillaria also has been confirmed, although they
are more distant than was previously thought. Cochemiea
appears to be basal to Mammillaria, which may prompt
systematists to recognize it as a segregate genus. Molecular
systematic studies to evaluate the extensive infrageneric
classification of Mammillaria also will determine whether
the morphological variants identified by traditional taxonomists are supported by genetically based DNA variation and therefore will provide valuable insights into the
speciation processes of recently diverged cactus groups.
Future studies of additional genera in the Cacteae will contribute to a better understanding of phylogenetic radiation
in Mexico and surrounding regions of this monophyletic
tribe.
Tribe Notocacteae is the South American counterpart
to Cacteae. This evolutionary branch includes a broad
array of low-growing barrel cacti native to various areas of
South America, including Chilean deserts, lowland grasslands of Argentina, southern Brazil, Paraguay, Uruguay,
and related habitats. Although not as diverse as Cacteae,
Notocacteae exhibit similar diversity in stem morphology,
with short solitary or clumping barrel forms. The Notocacteae include genera such as Blossfeldia, Copiapoa,
Eriosyce (including Neochilenia, Neoporteria, and Pyrrhocactus), Notocactus, Parodia, and perhaps Eulychnia, all
strictly South American lineages and likely derived from
ancestral populations arising farther north and west. Only
limited molecular study of the Notocacteae has been conducted, so the intergeneric relationships of this tribe are
still not well understood.
One central question to be resolved is whether the two
“barrel cactus” tribes (Cacteae and Notocacteae) arose from
a common ancestor during the early diversification of the
Cactoideae. If these tribes are determined to be sister
groups, the barrel cacti will then serve as a good example
for independent morphological evolution along different
paths on different continents that resulted in dissimilar
morphological solutions to similar evolutionary and environmental challenges. Furthermore, a phylogeny for the
Notocacteae could also shed light on the pattern of migration seen in southeastern South America, as well as establish evolutionary links of the isolated Atacama Desert
species to those purportedly related genera on the eastern
side of the Andes.
Solving Classification Problems Using Molecular Techniques
Data from cpDNA may also help cactus systematists to determine whether an oddball taxon should be treated as a
monotypic genus or placed into another genus. Within
subtribe Stenocereinae of the Pachycereeae occurs a mas-
Evolution and Systematics
11
sive candelabriform columnar cactus that Gibson (1991)
found to be structurally very distinct and proposed recognition as a monotypic genus, Isolatocereus Backeberg. However, this segregate is most commonly treated within the
genus Stenocereus, with which it shares synapomorphic silica bodies (Gibson and Horak 1978; Gibson et al. 1986).
Both cpDNA restriction site data (Cota and Wallace 1997)
and gene sequence data strongly support recognizing I. dumortieri as a monotype, basal to the tightly nested species
of Stenocereus (Fig. 1.4; Wallace 1995). Recognition of
Isolatocereus is also supported by a cladistic analysis based on
structural features (Cornejo and Simpson 1997).Fgiur4e1.neahrere:
Another example of generic realignments that benefit
from molecular systematic study is found in the genus
Harrisia (incl. Eriocereus and Roseocereus). This primarily
South American and Caribbean genus has previously been
classified in tribe Hylocereeae (Gibson and Nobel 1986;
Hunt and Taylor 1986) or in the Leptocereeae or Echinocereeae (Barthlott 1988; Hunt and Taylor 1990; Barthlott
and Hunt 1993). Studies of its plastid sequences for the
gene rbcL, the trnL–F intergenic spacer, and the rpl16 intron all indicate instead that this genus has its closest evolutionary affinities with members of the tribe Trichocereeae
in the BCT clade. Axillary hairs in the floral bracts are a
morphological synapomorphy for placement of Harrisia
into this tribe. Furthermore, Harrisia shares the 300 bp
deletion in Domain IV of the rpl16 intron observed in
members of the BCT clade, which eliminates the possibility that Harrisia should be assigned to either the Leptocereeae or Echinocereeae, which do not possess this
unique deletion. Thus, Harrisia may be confidently placed
within the Trichocereeae of the BCT clade.
Similar types of taxonomic placement problems can
also be resolved at the species level. A scandent, relatively
thin-stemmed cactus originally described as Mediocactus
hahnianus from Rio Apa, Brazil, was transferred to the
genus Harrisia by Kimnach (1987) based on morphological similarities — particularly of the flower and stem —
between this species and other members of Harrisia. A molecular systematic study of the interspecific relationships in
Harrisia (Wallace 1997) found that H. hahniana did not
fall within the well-supported Harrisia clade or with any
species of Mediocactus or Hylocereus (tribe Hylocereeae) but
allied strongly with members of the genera Trichocereus and
Echinopsis (also members of tribe Trichocereeae). Using the
comparative sequence data from the rpl16 intron that corroborated similarities of floral morphology, Wallace transferred H. hahnianus to the genus Echinopsis, now of the
BCT clade.
Presence or absence of a major structural rearrangement
12
Wallace and Gibson
is very useful in determining evolutionarily related groups
of taxa. Occurrence of the 300 bp deletion in the intron of
the plastid gene rpl16 is useful for including or excluding
taxa thought to be related to that clade. For example, the
columnar cactus Stetsonia coryne from Argentina may have
its closest affinities with members of Cereeae (Gibson and
Nobel 1986), not Leptocereeae (Barthlott and Hunt 1993);
members of the latter tribe do not share this 300 bp deletion. Similarly, Neoraimondia, Armatocereus, and the Galápagos Archipelago–endemic Jasminocereus thourarsii have
affinities with members of tribe Browningieae (Barthlott
and Hunt 1993), not Leptocereeae (Gibson and Nobel
1986). Further study of these relationships will broaden the
information base from which more robust hypotheses
about columnar cactus evolution and migration in South
America can be more reliably made.
Phylogenetic Studies of Subfamily Opuntioideae
Until very recently, most cactus systematists and hobbyist
cactus growers had focused little attention on classification
of the 250 species of Opuntioideae, or approximately 15%
of the family. This is regrettable because some opuntias are
dominant perennials in drylands of the New World or have
become weedy invaders elsewhere and spread by grazing
habits of livestock (Nobel 1994, 1998). Important food
sources are obtained from platyopuntias (Russell and Felker
1987). Understandably, gardeners generally elected not to
cultivate opuntias, which have nasty, irritating glochids and
are not easily controlled plants, but now, growing small opuntioids, especially taxa from western South America, has
become very popular among cactus enthusiasts.
Due to the relatively small amount of systematic research emphasis placed on the Opuntioideae by past researchers, a significant gap exists in our understanding of
the evolutionary relationships among these members of the
Cactaceae. Perhaps most important, an intensive phylogenetic analysis for this subfamily is required to evaluate the
generic circumscription. Cactus researchers especially need
to elucidate the early divergences of the opuntioid taxa to
understand how many distinct lineages have resulted in
North and South America, as well as what the generic
“boundaries” are for genera and subgenera. For example,
the relationships of the low-growth forms, such as in the
genera Maihueniopsis and Tephrocactus, have been extremely hard to predict on the basis of superficial examination of external characters, and the evolutionary history of structural transitions has been an area merely of
speculation.
A number of morphological transitions have been hypothesized for the opuntioid lineages. Two in particular are
Leptocereus
Acanthocereus
Leptocereeae
Pachycereus
Lophocereus
Carnegiea
Neobuxbaumia
Bergerocactus
Pachycereinae
Nyctocereus
Columnar
ancestor
Peniocereus
Pachycereeae
Echinocereus
Stenocereinae
Morangaya
?
?
Stenocereus
Escontria
Polaskia
Myrtillocactus
Isolatocereus
Corryocactus?
Figure 1.4. Hypothesized intergeneric relationships within some North American columnar cacti based on
analyses of rpl16 intron sequences. Tribe Pachycereeae appears to consist of two subtribes, Stenocereinae
and Pachycereinae (sensu Gibson and Horak 1978), but gene sequence analyses indicate that definitions of
both subtribes need to be expanded to include other species.
key: (1) a shift from persistent leaves to ephemeral foliage
leaves; and (2) changes in the shoot design from relatively
uniform, cylindrical succulent stems to jointed stems with
either cylindrical or flattened segments, i.e., cladodes (synonym, phylloclades). Another presumed trend has been a
shift in growth habit from upright woody plants (shrubs to
small trees) to shrubby or sprawling clumps, and even evolution of the geophytic habit in Pterocactus, in which most
plant biomass is subterranean and the aboveground parts
are annual shoots.
A factor that contributes considerably to the taxonomic confusion within the subfamily is the high level of
phenotypic plasticity shown within many opuntioid taxa.
In species with shoot features, different vegetative forms
have at times been given different scientific binomials,
adding to the nomenclatural problems of the group. Additionally, both polyploidy and hybridization have played a
vital role in the evolution of the diversity of these cacti and
have also contributed to nomenclatural chaos (Benson
1982). In fact, the Opuntioideae accounts for more than
Evolution and Systematics
13
75% of the polyploidy observed in the Cactaceae (Benson
1982).
Although Opuntioideae present a considerable challenge to the cactus systematist, recent studies have provided much insight into opuntioid evolution. Of critical importance is sharply defining the generic concept for the
genus Opuntia. In some classifications, Opuntia represents
a wide array of small terete-stemmed trees, shrubs, plants
with dwarf and clump-forming habits, chollas, club chollas, platyopuntias (prickly pears), and the tree opuntias of
Brazil and the Caribbean. In other classifications, these
same plants may be reclassified into ten or more genera.
Some morphologically distinct plants, such as the geophytic species of Pterocactus in Argentina or the persistent
leaf-bearing species Pereskiopsis and Quiabentia of North
and South America, respectively, are more readily distinguished as segregate genera. But even here, Pereskiopsis and
Quiabentia have been lumped into a single genus (Hunt
and Taylor 1990).
Studies of seed morphology and other aspects of micromorphology have provided evidence that a complete
reevaluation of the generic circumscriptions in the subfamily is warranted (Stuppy 2002). Molecular systematic
studies by Dickie (1998) and Dickie and Wallace (2001)
were specifically designed to address these generic circumscription problems. From studies of plastid DNA variation
(rbcL, trnL–F intergenic spacer, rpl16 intron), the inferred
phylogeny indicated that there were five clades within the
subfamily, related both geographically and morphologically (Fig. 1.5), which follows the structural evidence detailed by Stuppy (2002). A basal lineage for the subfamily
appears to include the species referable to the genera
Austrocylindropuntia and Cumulopuntia, both native to the
Peru-Bolivia-Chile Andean regions. Other clades are the
narrowly distributed South American Pterocactus; a clade
of Maihueniopsis-Tephrocactus (including Puna); and two
clades containing the more widely distributed opuntioids
found in both North and South America. The first of these
more diverse clades is the “cylindroid” lineage, showing a
south to north grade of specialization from leafy, cylindrical-stemmed ancestral forms such as Pereskiopsis and
Quiabentia of North and South America, respectively, to
more specialized, segmented-stemmed chollas of North
America (Grusonia [including Marenopuntia, Micropuntia,
and Corynopuntia] and Cylindropuntia).Fgiur5en1.eahrere:
For the flat-stemmed opuntioid taxa, a similar but
more subtle south-to-north transition is seen, beginning
with the plesiomorphic genus Miqueliopuntia of the
Atacama Desert. Here terete-stemmed, clump-forming
opuntioids (in contrast to the solitary terete stems of
14
Wallace and Gibson
Austrocylindropuntia) tend to grade into plants with
flattened stems, as in Airampoa, which form the basal lineages of the platyopuntia clade. Forest emergents, such as
in Brasiliopuntia and Consolea of Brazil and the Caribbean,
respectively, also show morphological transitions from
terete stems of their trunks to flattened leaflike phylloclades (“pads”). These stem joints are seasonally deciduous
in Brasiliopuntia. The true platyopuntias (genus Opuntia
in the type sense) have experienced complete loss of cylindrical stems, except in seedling stages. One notable exception in the caatinga of eastern Brazil is Tacinga funalis, a
scrambling, thin-stemmed subshrub that has reverted to
entirely terete stems, despite its clear affinities with flatstemmed prickly pears, as determined by molecular data.
The taxonomic dilemma is that the majority of the genera discussed here have typically been subsumed into a
“catch-all” genus, Opuntia. The molecular data have made
it possible to determine evolutionarily related groups (e.g.,
five major clades) and has provided sufficient evolutionary
information about these lineages to construct a robust phylogeny. The intergeneric groups defined by the molecular
studies of Dickie and Wallace (2001) are essentially the same
generic groups that Stuppy (2002) proposed based on studies of seed structures, in that both suggest that approximately 12 to 15 genera should be recognized as monophyletic
units within the subfamily. Furthermore, the morphological discontinuities observed between these opuntioid genera
are, in reality, greater than those now recognized between
members of tribes in Cactoideae (e.g., the tribe Cacteae),
whose generic distinctions have only rarely been questioned.
Opuntioideae, therefore, offer a critical test for cactus
systematics. Many researchers, for convenience, would prefer to have fewer and larger genera, but many smaller genera may have to be recognized to represent the true evolutionary lineages. Whether all or none of these smaller,
demonstrably monophyletic groups are recognized at the
rank of genus, subtribes, or tribes by cactus systematists remains to be seen. Discussions will eventually resolve these
questions and incorporate the available data and conclusions into a practical and generally accepted classification
for the Opuntioideae. Without a reliable phylogeny to
form the basis of systematic comparisons, such discussions
and interpretations of morphological variation would be
very problematic, if possible at all.
New Insights into Cactus Evolution
Structural Properties
Having even the current, crude phylogenetic knowledge
from molecular systematic studies has provided new in-
Majority Rule
Maihuenia
Pereskia
Opuntia subulata
O. pachypus
Austrocylindropuntia
O. echinacea
Cumulopuntia
O. kuehnrichiana
Pterocactus kuntzei
Pterocactus
Opuntia bradtiana
Grusonia
O. clavata
O. stanlyi
O. marenae
Corynopuntia
Marenopuntia
O. caribaea
Cylindropuntia
O. spinosior
Pereskiopsis porteri
Pereskiopsis
P. aquosa
Quiabentia pflanzii
Quiabentia
Q. verticillata
Opuntia weberi
O. nigrispina
Tephrocactus
O. molinensis
O. clavarioides
Maihueniopsis
O. atacamensis
O. miquelii
Miqueliopuntia
O. tilcarensis
Airampoa
O. brasiliensis
Brasiliopuntia
O. chaffeyi
O. guatemalensis
O. phaeacantha
Opuntia
O. polycantha
O. palmadora
O. inamonea
Tacinga funalis
Tacinga
T. braunii
Opuntia falcata
Consolea
O. spinosissima
Figure 1.5. Strict consensus tree of 32,700 equally parsimonious trees from analysis of rpl16 intron sequences in
the subfamily Opuntioideae (Dickey and Wallace 2000). The analysis strongly supports recognizing many of
the segregate genera formerly proposed for opuntioids.
sight into how the structure of cacti has evolved. Perhaps
gone will be the methods of using anatomical data to devise phylogenetic hypotheses. For example, Gibson and
Horak (1978) used the presence of calcium oxalate crystals
in the skin (epidermis and collenchymatous hypodermis)
of the stem to indicate that certain species of Mexican
columnar cacti are closely related and therefore not members of subtribe Stenocereinae (Pachycereeae). Some of the
North American species possessing these calcium oxalate
crystals were classified in the genus Cephalocereus. Zappi
(1994) monographed the genus Pilosocereus (tribe Cereeae),
which is greatly developed in Brazil, but which includes
certain North America species of cephalocerei. The occurrence of such crystals (Fig. 1.6) now appears to be a shared
primitive feature (symplesiomorphy) found in many of the
basal taxa, e.g., Leptocereeae and Hylocereeae, as well as in
subfamilies Opuntioideae and Cereeae (Gibson and Horak
1978; Mauseth and Ross 1988; Mauseth 1996; Mauseth et
al. 1998). Therefore, presence of such crystals may instead
be an ancient character of the Cactaceae. Furthermore,
other Portulacineae have epidermal calcium oxalate features (Gibson 1994), suggesting an even older origin of that
character, and probably indicating that crystals have been
evolutionarily lost in a number of lineages. This permits researchers to determine where regulatory genes first arose to
yield a character.Fgiur6e1.neahrere:
A phylogenetic reconstruction of Cactaceae will elucidate where features first evolved in cacti. For example, did
the collenchymatous hypodermis typical of subfamilies
Opuntioideae and Cactoideae evolve once from a common
ancestor or twice independently? A medullary vascular system occurs in many, but not all, Cactoideae (Gibson and
Horak 1978; Mauseth 1993b), and a molecular phylogeny
may determine whether absence of medullary bundles in
any species of the family is a primitive or a derived, lost
character state. Narrow pith and absence of mucilage cells
have been treated as primitive characters for cactus stems,
and this can also be tested by character mapping on cladistic models.
Revised Biogeographic Models Based on Molecular Studies
Evolution of the cacti from ancestral populations of portulacaceous ancestors is being supported again and again
by molecular studies of a variety of genes and other DNA
sequences (Applequist and Wallace 2000). Previous hypotheses about the center of origin and dispersal of the
Cactaceae have been reviewed (Gibson and Nobel 1986),
but conflicting concepts remain as to whether the “northwest South America” hypothesis or the “Caribbean origin”
hypothesis should prevail.
16
Wallace and Gibson
The family-wide studies of DNA variation, as well as
more intensive studies in subfamilies and tribes, begin to
converge on a single, different hypothesis that is consistent
with the early Gondwanan ancestry of the families of the
Caryophyllales, and that of paleoclimatic and paleogeologic activities in western South America. When the phylogenies are determined for each of the various major cactus clades investigated, the recurrent observation is that the
basal groups, presumably representing the plesiomorphic
lineages, today inhabit the central Andean region of northern Chile, northwest Argentina, Bolivia, and Peru. Of
those relevant systematic studies completed on cactus taxa
with representatives in this region, the plesiomorphic
members are invariably found here. This is true for the
genera Pereskia, Harrisia, and Lepismium, as well as for the
tribe Rhipsalidae, the subfamily Opuntioideae (Austrocylindropuntia and Cumulopuntia basal), and the subfamily Cactoideae (Calymmanthium basal). Because the
plesiomorphic taxa of presumably independent lineages
(following divergence from a common ancestor) are still
found in this general region, it is reasonable to assume that
this represents the center of origin or earliest radiation for
the Cactaceae. Other studies of South American flora (e.g.,
Raven and Axelrod 1974) cite the importance of this region
as a source for diversification in numerous angiosperm lineages, noting the importance of the Andean orogeny in
shaping the migration pathways of the resultant diverged
lineages.
The proposed scenario for the origin and diversification of cacti begins with its divergence from Portulacaor Talinum-like ancestors, perhaps in the Upper Cretaceous after the breakup of Gondwana and the isolation of
South America from the remaining austral continents
about 110 million years ago. Ancestral populations ultimately became more succulent and “stem-dominant,”
with a concomitant reduction in the importance of leaves
as Crassulacean acid metabolism (Chapter 4) became the
dominant photosynthetic pathway. As the major lineages
(subfamilies) diverged, they also appear to have followed
three main migration paths, one to the north, one to the
south along the Andean Cordillera, and another from west
to east to establish another center of diversification in eastern Brazil. Examples of southerly migrating groups are
Maihuenia, Pterocactus, and members of tribes Trichocereeae and Notocacteae. Eastward migrants include members of tribes Rhipsalideae and Cereeae, Harrisia, and some
Opuntioideae, notably Brasiliopuntia and the caatinga
species of the Opuntia inamoena complex, including both
species of Tacinga. Following a northward migration were
a number of cylindrical opuntioid lineages arising from
A
B
Figure 1.6. Scanning electron photomicrographs of calcium oxalate crystals in the skin of two columnar cacti: (A) Mitrocereus fulviceps, and (B)
Armatocereus laetus. Scale bars = 10 µm.
Austrocylindropuntia and Quiabentia, resulting in divergences of the cholla lineages of North America (Cylindropuntia and Grusonia). A parallel situation also occurred in
flat-stemmed Opuntia taxa, where the prickly pears
(Opuntia sensu stricta) diversified on the mainland while
the genus Consolea arose in the Caribbean.
In subfamily Cactoideae, three major lineages migrated north (presumably along the Andean corridor) until
they reached Central America and the Caribbean islands
(which were located much farther west of their present location). From what likely were Corryocactus-like ancestors,
the divergence of tribes Pachycereeae (mainland) and
Leptocereeae (Caribbean) occurred in this region (paralleling the Opuntia example above). The barrel-cactus
forms seen in tribe Cacteae (predominantly mainland) radiated rapidly and migrated to inhabit a wide geographic
range in North America, extending as far north as Canada.
Epiphytic cacti of tribe Hylocereeae were under environmental constraints to inhabit more mesic habitats and thus
remained in Central America and northern South America, limited by water availability and moderate temperature
requirements.
The evolutionary scenario presented above is at least
consistent with the paleogeographic conditions over the
last 60 million years, and the relationships of the various
cactus groups are supported by several independent studies of different groups of cacti and using different molecular markers. As more molecular and morphological data
accrue from future studies of cactus evolution and systematics, the hypotheses presented here regarding the biogeographic history of the family may be further refined or
even rejected. At present, these are the best working hypotheses for the origins of the various lineages within the
family and why they are distributed in their present geographic patterns.
Concluding Remarks
The cactus community is composed of a great diversity of
users, and most are keenly interested in classification
below the level of the subfamily, needing correct binomials and the assignment of each species to the proper genus.
Above all, there is a great need for stabilization of names in
the Cactaceae (Hunt and Taylor 1986, 1990; Hunt 1991). At
the same time, scientists absolutely require a phylogeny of
the family so that applications can be made for understanding the evolution of characters and their various
states, as well as understanding the processes of speciation,
biogeographic radiation, and the evolution of cacti as host
plants for other organisms.
Although a cladistic approach to classification may appear destabilizing at first, a much more stable system of
classification should be produced soon. Questions about
assigning a plant to a genus can become pro forma and inexpensive using genetic markers. It would not be unreasonable for the cactus community to require routine genetic testing of new taxa before publication to avoid
Evolution and Systematics
17
confusion produced from structural analyses. The major
issue then confronting an author would be whether the
population is assignable to a previously described species or
is new to science.
Given the present understanding of the evolutionary
patterns in the Cactaceae, considerable research is still
needed to address questions of generic delimitation, tribal circumscription, and species identity. Integration of
molecular, morphological, and biogeographic data will undoubtedly bring about a more robust and useful perspective on relationships within the Cactaceae and, to users of
these data, a more stable and reliable source of biological
information about this diverse and exceptional family.
Literature Cited
Angiosperm Phylogeny Group. 1998. An ordinal classification for the families of flowering plants. Annals of
the Missouri Botanical Garden 85: 531–553.
Appleqvist, W. L., and R. S. Wallace. 2001. Phylogeny of
the portulacaceous cohort based on ndhF gene sequence data. Systematic Botany 26: 406–419.
Backeberg, C. 1962. Cactaceae, Vol. 6. Gustav FischerVerlag, Jena, East Germany.
Backeberg, C. 1976. Cactus Lexicon, 3rd ed. Blandford
Press, Poole, Dorset, United Kingdom.
Bailey, I. W. 1960. Comparative anatomy of the leaf-bearing Cactaceae. I. Foliar vasculature of Pereskia,
Pereskiopsis, and Quiabentia. Journal of the Arnold
Arboretum 41: 341–356.
Bailey, I. W. 1964. Comparative anatomy of the leafbearing Cactaceae. XI. The xylem of Pereskiopsis and
Quiabentia. Journal of the Arnold Arboretum 45: 140–
157.
Bailey, I. W. 1966. The significance of the reduction of vessels in the Cactaceae. Journal of the Arnold Arboretum
47: 288–292.
Barthlott, W. 1983. Biogeography and evolution in neoand paleotropical Rhipsalinae. In Dispersal and
Distribution (K. Kubitzki, ed.). Verlag Paul Parey,
Hamburg, Germany. Pp. 241–248.
18
Barthlott, W., and S. Porembski. 1996. Ecology and morphology of Blossfeldia liliputana (Cactaceae): a poikilohydric and almost astomate succulent. Botanica
Acta 109: 161–166.
Barthlott, W., S. Porembski, M. Kluge, J. Hopke, and L.
Schmidt. 1997. Selenicereus wittii (Cactaceae): an epiphyte adapted to Amazonian Igapó inundation forests.
Plant Systematics and Evolution 206: 175–185.
Behnke, H.-D. 1976a. Die Siebelement Plastiden der
Caryophyllaceae, eine weitere spezifische Form der
P-Typ Plastiden bei Centrospermen. Botanische Jahrbücher für Systematik 95: 327–333.
Behnke, H.-D. 1976b. Ultrastructure of sieve-element
plastids in Caryophyllales (Centrospermae), evidence
for the delimitation and classification of the order.
Plant Systematics and Evolution 126: 31–54.
Behnke, H.-D. 1994. Sieve-element plastids. In Caryophyllales: Evolution and Systematics (H.-D. Behnke
and T. J. Mabry, eds.). Springer-Verlag, Heidelberg,
Germany. Pp. 87–121.
Benson, L. 1982. The Cacti of the United States and Canada.
Stanford University Press, Stanford, California.
Boke, N. H. 1944. Histogenesis of the leaf and areole in
Opuntia cylindrica. American Journal of Botany 31:
299–316.
Boke, N. H. 1954. Organogenesis of the vegetative shoot in
Pereskia. American Journal of Botany 41: 619–637.
Bregman, R. 1988. Forms of seed dispersal in Cactaceae.
Acta Botanica Neerlandica 37: 395–402.
Bregman, R. 1992. Seed studies in the subtribe Borzicactinae Buxbaum (Cactaceae); morphology, taxonomy,
phylogeny and biogeography. Botanische Jahrbücher für
Systematik 114: 201–250.
Butterworth, C. A., and R. S. Wallace. 1999. Phylogenetic
studies of the tribe Cacteae. Cactaceae Consensus
Initiatives 8: 7–9.
Barthlott, W. 1987. New names in Rhipsalidinae (Cactaceae). Bradleya 5: 97–100.
Butterworth, C. A., J. H. Cota-Sanchez, and R. S.
Wallace. 2002. Molecular systematics of Tribe
Cacteae (Cactaceae: Cactoideae): A phylogeny based
on rpl16 intron sequence variation. Systematic Botany
(in press).
Barthlott, W. 1988. Über die systematischen Gliederungen
der Cactaceae. Beitrage Biologie der Pflanzen 63: 17–40.
Buxbaum, F. 1950. Morphology of Cacti. Section I. Roots and
stems. Abbey Garden Press, Pasadena, California.
Barthlott, W., and D. R. Hunt. 1993. Cactaceae. In The
Families and Genera of Vascular Plants, Vol. 2. (K.
Kubitzki, J. G. Rohwer, and V. Bittrich, eds.). SpringerVerlag, Berlin. Pp. 161–197.
Buxbaum, F. 1953. Morphology of Cacti. Section II. The
Flower. Abbey Garden Press, Pasadena, California.
Wallace and Gibson
Buxbaum, F. 1955. Morphology of Cacti. Section III. Fruits
and Seeds. Abbey Garden Press, Pasadena, California.
Buxbaum, F. 1958. The phylogenetic division of the subfamily Cereoideae, Cactaceae. Madroño 14: 177–206.
ORF2280 homolog sequences. American Journal of
Botany 84: 253–273.
Buxbaum, F. 1966. The origin of the tribe Browningieae.
Cactus and Succulent Journal (U.S.) 38: 43–46.
Eichler, A. W. 1878. Blümendiagramme, Part 2. Engelmann,
Leipzig, East Germany.
Clements, J. S., T. J. Mabry, H. Wyler, and A. S. Dreiding.
1994. Chemical review and evolutionary significance of
the betalains. In Caryophyllales: Evolution and
Systematics (H.-D. Behnke and T. J. Mabry, eds.).
Springer-Verlag, Heidelberg, Germany. Pp. 247–261.
Gibson, A. C. 1973. Comparative anatomy of secondary
xylem in Cactoideae (Cactaceae). Biotropica 5: 29–65.
Cornejo, D. O., and B. B. Simpson. 1997. Analysis of form
and function in North American columnar cacti (tribe
Pachycereeae). American Journal of Botany 84: 1482–
1501.
Cota, J. H., and R. S. Wallace. 1996. La citologia y la sistematica molecular en la familia Cactaceae. Cactáceas
y Suculentas Mexicanas 41: 27–44.
Cota, J. H., and R. S. Wallace. 1997. Chloroplast DNA evidence for divergence in Ferocactus and its relationships
to North American columnar cacti (Cactaceae: Cactoideae). Systematic Botany 22: 529–542.
Cronquist, A. 1981. An Integrated System of Classification of
Flowering Plants. Columbia University Press, New
York.
Gibson, A. C. 1976. Vascular organization of shoots of
Cactaceae. I. Development and morphology of primary vasculature in Pereskioideae and Opuntioideae.
American Journal of Botany 63: 414–426.
Gibson, A. C. 1977a. Wood anatomy of opuntias with
cylindrical to globular stems. Botanical Gazette 138:
334–351.
Gibson, A. C. 1977b. Vegetative anatomy of Maihuenia
(Cactaceae) with some theoretical discussions of ontogenetic changes in xylem cell types. Bulletin of the
Torrey Botanical Club 104: 35–48.
Gibson, A. C. 1978. Wood anatomy of platyopuntias.
Aliso 9: 279–303.
Gibson, A. C. 1982. Phylogenetic relationships of Pachycereeae. In Ecological Genetics and Evolution. The
Cactus-Yeast-Drosophila Model System ( J. S. F. Barker
and W. T. Starmer, eds.). Academic Press, Sydney. Pp.
3–16.
Cronquist, A., and R. F. Thorne. 1994. Nomenclature and
taxonomic history. In Caryophyllales: Evolution and
Systematics (H.-D. Behnke and T. J. Mabry, eds.).
Springer-Verlag, Heidelberg, Germany. Pp. 5–25.
Gibson, A. C. 1988a. The systematics and evolution of subtribe Stenocereinae. 3. Myrtillocactus. Cactus and Succulent Journal (U.S.) 60: 109–116.
Dickie, S. L. 1998. Phylogeny and evolution in the subfamily Opuntioideae (Cactaceae). Master’s Thesis,
Iowa State University, Ames.
Gibson, A. C. 1988b. The systematics and evolution of subtribe Stenocereinae. 5. Cina and its relatives. Cactus and
Succulent Journal (U.S.) 60: 283–288.
Dickie, S. L., and R. S. Wallace. 2001. Phylogeny and evolution in the subfamily Opuntioideae (Cactaceae): insights from rpl16 intron sequence variation. Systematic
Botany (in review).
Gibson, A. C. 1991. The systematics and evolution of subtribe Stenocereinae. 11. Stenocereus dumortieri versus
Isolatocereus dumortieri. Cactus and Succulent Journal
(U.S.) 63: 184–190.
Downie, S. R., and J. D. Palmer. 1993. A chloroplast
DNA phylogeny of the Caryophyllales based on structural and inverted repeat restriction site variation.
Systematic Botany 19: 236–252.
Gibson, A. C. 1994. Vascular tissues. In Caryophyllales:
Evolution and Systematics (H.-D. Behnke and T. J.
Mabry, eds.). Springer-Verlag, Heidelberg, Germany.
Pp. 45–74.
Downie, S. R., and J. D. Palmer. 1994. Phylogenetic relationships using restriction site variation of the chloroplast DNA inverted repeater. In Caryophyllales:
Evolution and Systematics (H.-D. Behnke and T. J.
Mabry, eds.). Springer-Verlag, Heidelberg, Germany.
Pp. 223–233.
Gibson, A. C., and K. E. Horak. 1978. Systematic anatomy and phylogeny of Mexican columnar cacti. Annals
of the Missouri Botanical Garden 65: 999–1057.
Downie, S. R., D. S. Katz-Downie, and K.-J. Cho. 1997.
Relationships in the Caryophyllales as suggested by
phylogenetic analyses of partial chloroplast DNA
Gibson, A. C., and P. S. Nobel. 1986. The Cactus Primer.
Harvard University Press, Cambridge, Massachusetts.
Gibson, A. C., K. C. Spencer, R. Bajaj, and J. L.
McLaughlin. 1986. The ever-changing landscape of
cactus systematics. Annals of the Missouri Botanical
Garden 73: 532–555.
Evolution and Systematics
19
Hershkovitz, M. A. 1991. Leaf morphology of Cistanthe
Spach (Portulacaceae). Annals of the Missouri Botanical
Garden 78: 1022–1060.
Hershkovitz, M. A., and E. A. Zimmer. 1997. On the evolutionary origins of the cacti. Taxon 46: 217–232.
Hunt, D. R. 1991. Stabilization of names in succulent
plants. Regnum Vegetabile 123: 151–155.
Mauseth, J. D., and R. G. Ross. 1988. Systematic anatomy
of the primitive cereoid cactus, Leptocereus quadricostatus. Bradleya 6: 49–64.
Hunt, D. R., and N. P. Taylor (eds.). 1986. The genera of
Cactaceae: towards a new consensus. Bradleya 4: 65–78.
Mauseth, J. D., Y. Uozumi, B. J. Plemons, and J. V.
Landrum. 1995. Structural and systematic study of an
unusual tracheid type in cacti. Journal of Plant Research
108: 517–526.
Hunt, D. R., and N. P. Taylor (eds.). 1990. The genera of
Cactaceae: progress towards consensus. Bradleya 8: 85–
107.
Mauseth, J. D., T. Terrazas, and S. Loza-Cornejo. 1998.
Anatomy of relictual members of subfamily Cactoideae, IOS group 1a (Cactaceae). Bradleya 16: 31–43.
Kiesling, R. 1988. Cactaceae. In Flora Patagónica 5,
Dicotiledoneas dialipétalas (Oxalidaceae a Cornaceae)
(M. N. Correa, ed.). Coleccion Científica del INTA 8,
Buenos Aires, Argentina. Pp. 218–243.
Nobel, P. S. 1988. Environmental Biology of Agaves and
Cacti. Cambridge University Press, New York.
Kimnach, M. 1987. Harrisia hahniana. Cactus and Succulent Journal (U.S.) 59: 59–62.
Nobel, P. S. 1991. Tansley Reveiw 32. Achievable productivities of CAM plants: basis for high values compared
with C3 and C4 plants. The New Phytologist 119: 183–
205.
Leuenberger, B. E. 1976. Die Pollenmorphologie der
Cactaceae und ihre Bedeutung für die Systematik mit
Berträgen zur Methodik rasterelektronenmikroskopischer. Dissertationes Botanicae 31: 1–321.
Nobel, P. S. 1994. Remarkable Agaves and Cacti. Oxford
University Press, New York.
Leuenberger, B. E. 1986. Pereskia (Cactaceae). Memoirs of
the New York Botanical Garden 41: 1–141.
Porembski, S. 1996. Functional morphology of Aztekium
ritteri (Cactaceae). Botanica Acta 109: 167–171.
Leuenberger, B. E. 1997. Maihuenia—monograph of a
Patagonian genus of Cactaceae. Botanische Jahrbücher
für Systematik 119: 1–92.
Raven, P. H., and D. I. Axelrod. 1974. Angiosperm biogeography and past continental movements. Annals of
the Missouri Botanical Garden 61: 539–673.
Mabry, T. J. 1964. The betacyanins, a new class of redviolet pigments, and their phylogenetic significance. In
Taxonomic Biochemistry and Serology (C. A. Leone,
ed.). Ronald Press, New York. Pp. 239–254.
Rettig, J. H., H. D. Wilson, and J. R. Manhart. 1992.
Phylogeny of the Caryophyllales—gene sequence data.
Taxon 41: 201–209.
Manhart, J. R. and J. H. Rettig. 1994. Gene sequence data.
In Caryophyllales: Evolution and Systematics (H.-D.
Behnke and T. J. Mabry, eds.). Springer-Verlag,
Heidelberg, Germany. Pp. 235–246.
Mauseth, J. D. 1993a. Water-storing and cavitation-preventing adaptations in wood of cacti. Annals of Botany
72: 81–89.
Mauseth, J. D. 1993b. Medullary bundles and the evolution of cacti. American Journal of Botany 80: 928–932.
Mauseth, J. D. 1995. Collapsible water-storage cells in
cacti. Bulletin of the Torrey Botanical Club 122: 145–151.
Mauseth, J. D. 1996. Comparative anatomy of tribes
Cereeae and Browningieae (Cactaceae). Bradleya 14:
66–81.
Mauseth, J. D., and B. J. Plemons. 1995. Developmentally
20
variable, polymorphic woods in cacti. American Journal
of Botany 82: 1199–1205.
Wallace and Gibson
Nobel, P. S. 1998. Los Incomparables Agaves y Cactos.
Editorial Trillas, Mexico City.
Rodman, J. E., M. K. Oliver, R. R. Nakamura, J. U.
McClammer, and A. N. Bledsoe. 1984. A taxonomic
analysis and revised classification of the Centrospermae. Systematic Botany 9: 297–323.
Rose, M.-J., and W. Barthlott. 1994. Coloured pollen in
Cactaceae: a mimetic adaptation to hummingbirdpollination? Botanica Acta 107: 402–406.
Rowley, G. D. 1976. A Lophophora mimic—Matucana
madisoniorum. Excelsa 6: 93–94.
Russell, C. E., and P. Felker. 1987. The prickly-pears
(Opuntia spp., Cactaceae): A source of human and animal food in semiarid areas. Economic Botany 41: 433–
445.
Saveja, M., and J. D. Mauseth. 1991. Leaf-like structure in
the photosynthetic, succulent stems of cacti. Annals of
Botany 68: 405–411.
Speirs, D.C. 1982. The cacti of western Canada (part 3).
National Cactus and Succulent Journal 37: 53–54.
Stuppy, W. 2002. Seed characters and generic delimitation
in subfamily Opuntioideae. Succulent Plant Research
(in press).
Thorne, R. F. 1973. Floristic relationships between tropical
Africa and tropical America. In Tropical Forest Ecosystems in Africa and South America: a Comparative
Review (B. J. Meggars, E. S. Ayensu, and W. D.
Duckworth, eds.). Smithsonian Institute Press, Washington, D.C. Pp. 27–47.
Thorne, R. F. 1983. Proposed new realignments in the angiosperms. Nordic Journal of Botany 3: 85–117.
Turner, B. L. 1973. Chemosystematic data: their use in the
study of disjunctions. Annals of the Missouri Botanical
Garden 59: 152–164.
Wallace, R. S. 1995. Molecular systematic study of the
Cactaceae: using chloroplast DNA variation to elucidate cactus phylogeny. Bradleya 13: 1–12.
Wallace, R. S. 1997. The phylogenetic position of Mediocactus hahnianus. Cactaceae Consensus Initiatives 4: 11–
12.
Wallace, R. S. 2002. Evidence for the recognition of a
fourth subfamily of Cactaceae: Maihuenioideae. Plant
Systematics and Evolution (in review).
Wallace, R. S., and J. H. Cota. 1996. An intron loss in the
chloroplast gene rpoC1 supports a monophyletic origin
for the subfamily Cactoideae. Current Genetics 29:
275–281.
Wallace, R. S., and E. D. Forquer. 1995. Molecular evidence for the systematic placement of Echinocereus pensilis (K. Brandegee) J. Purpus (Cactaceae: Cactoideae:
Echinocereeae). Haseltonia 3: 71–76.
Zappi, D. C. 1994. Pilosocereus (Cactaceae). The genus in
Brazil. Succulent Plant Research 3. David Hunt, Dorset,
United Kingdom.
Evolution and Systematics
21
CHAPTER
›2‹
S H O OT A N ATO M Y
A N D M O R PH O LO G Y
Teresa Terrazas Salgado and James D. Mauseth
Introduction
Epidermis and Hypodermis
Fundamental Tissue
Chlorenchyma
Inner Cortex
Pith
Mucilage Cells, Laticifers, and Sclereids
Mineral Inclusions
Vascular Tissue
Cortical and Medullary Bundles
Xylem
Phloem
Wood
Morphology
Biomechanics
Conclusions and Future Prospects
Literature Cited
Introduction
Basic anatomical features of Cactaceae have been studied
since the 16th century (Metcalfe and Chalk 1950; Conde
1975). More recently, other features have been observed for
cultivated plants, such as variations in cuticle thickness, number of hypodermal cell layers, and hypodermal wall thickness
(Nyffeler and Eglii 1997). Boosfeld (1920) was one of the first
to emphasize the correlation of internal anatomy with external form, noting that taxa that have very different external
forms can also have very different internal structure. Among
the modifications accompanying the evolution of cacti from
leafy ancestors that employ C3 photosynthesis to stem-photosynthetic Crassulacean acid metabolism (CAM) succulents
(Chapter 1) are stems with an increased stomatal frequency,
a palisade cortex, a large internal surface area due to extensive
intercellular spaces, cortical and medullary vascular bundles,
wood modifications, and atypical pith features. The wood
not only contains the water-conducting tissue (vessels in the
xylem) but can also function in support and affect the shape
of cacti. In turn, the shape helps dictate the biomechanical
properties of the shoot.
23
Epidermis and Hypodermis
The epidermis is the outermost layer of cells through
which all exchanges with the environment occur; it also
provides important taxonomic characters to help distinguish between closely related genera, e.g., Encephalocarpus
and Pelecyphora (Boke 1959) or species, e.g., Neoevansia
striata and N. zopilotensis (Herrera-Cardenas et al. 2000).
A typical cactus stem generally has a uniseriate (one cell
layer thick) epidermis with square or rectangular cells in
transverse section. Subsequent epidermal cell divisions
parallel to the periclinal (external) walls produce a distinctive multiseriate epidermis in some species of certain
genera, including Astrophytum, Eriosyce, Eulychnia, and
Pachycereus. In other taxa, epidermal cell divisions lack a
definitive orientation parallel to the periclinal walls, occur
in various angles, and may have divisions only in patches
rather than for all epidermal cells (Mauseth 1996; Nyffeler
and Eggli 1997).
Most cactus species possess thin-walled epidermal cells;
however, for a few taxa, such as species of Armatocereus,
Cereus, Jasminocereus, and Mammillaria, the periclinal (external) wall is thicker than the internal and radial walls
(Mauseth 1996; Loza-Cornejo and Terrazas 2001). The periclinal epidermal cell wall may be flat or convex. Convex
projections are recognized in several species of Ariocarpus,
Ferocactus, Lophophora, Opuntia, Peniocereus, Thelocactus,
and Turbinicarpus. For other genera, the convex outer surface is caused by a cell that divides repeatedly in different
planes to produce a cluster of epidermal cells (Fig. 2.1A).
This type of rough epidermis occurs in several members of
the Cactoideae, e.g., Eriosyce (Nyffeler and Eggli 1997),
Polaskia (Gibson and Horak 1978), and Browningia
(Mauseth 1996). Modifications in the hypodermis of
Uebelmannia (Mauseth 1984a) also lead to a rough epidermis. Convex projections in the form of papillae arising from
a single epidermal cell or as a series of cells can affect transpiration by influencing the boundary layer of air adjacent
to a stem surface (Fahn 1986; Nobel 1999).Fgiure2n1.eahrer:
The hydrophobic cuticle that forms on the external
wall of epidermal cells (and often on the internal wall) contains cutin, a mixture of fatty acids that polymerize on exposure to oxygen. Typically, the fatty acids are produced in
the protoplasm and then migrate through the plasma
membrane and the cell wall. The cuticle commonly is
smooth, but in some cacti it is rough and thick, as in
Ariocarpus fissuratus (Fig. 2.1B). Young epidermal cells near
the stem apex are covered by a thin cuticle, but older epidermal cells usually have a thick cuticle when compared
with typical dicotyledons. Cuticle thickness varies from
24
Terrazas Salgado and Mauseth
1 µm to more than 200 µm in species of Cactoideae (LozaCornejo and Terrazas 2001) and from 8 to 58 µm for species
of Opuntia (Pimienta-Barrios et al. 1993). Variations in cuticular thickness may be related to the water conserving
ability of a species, although a relationship between cuticle thickness and water-stress resistance has not been observed for opuntias (Pimienta-Barrios et al. 1993). A thick
cuticle may also increase the reflection of radiation, which
will reduce stem temperatures (Nobel 1999).
As indicated, a cuticle can occur on the inner side of
epidermal cells, as for Homalocephala texenis and Uebelmannia gummosa (Mauseth 1984a). Also, the cuticle can
penetrate deeply into the anticlinal (radial) walls, as for
Armatocereus, Bergerocactus, Echinocereus, Escontria, Myrtillocactus, Nopalea, Oreocereus, and Pereskia (Gibson and
Horak 1978; Mauseth 1984a, 1996; Loza-Cornejo and
Terrazas 2001). Another way epidermal cells provide extra
protection is for the protoplasm to produce long-chain
fatty acids, which polymerize into wax. These also migrate
to the outer surface of the external wall and are deposited
on the existing cuticle. This epicuticular wax layer can be
smooth or consist of particles of diverse sizes and shapes,
such as aggregated beads, flakes, or threads (Mauseth
1984a) and is responsible for the grayish or bluish color of
certain cactus stems (Gibson and Nobel 1986).
The only physical openings in the epidermis for the
exchange of gases with the surrounding air are the stomata;
the aperture of each stoma is controlled by two guard cells.
Frequently, the stomata and guard cells are at the same
level as the other epidermal cells, but sometimes they are
located at the bottom of a pit or depression (Mauseth
1984a). In some species, the cuticle on mature tissue is
greatly thickened and causes an increase in the distance of
the stomata from the turbulent air, which makes the stomata appear sunken. When stomata are at the bottom of
pits or surrounded by thick cuticle, the resistance to water
vapor loss is increased slightly (Nobel 1999). Species of
Maihuenia, Pereskia, Pereskiopsis, and Quiabentia possess
stomata mainly in their leaves (or near the areoles; Mauseth
1999a), whereas most Opuntioideae and Cactoideae have
stomata mainly in their stem epidermis. A few species of
Cactoideae have stomata restricted to certain regions of the
stem, as in the valleys between the ribs or on the edges of
the tubercles (Gibson and Nobel 1986; Porembski 1996;
Loza-Cornejo and Terrazas 1996; Herrera-Cardenas et al.
2001), and stomata are absent in the epidermis of certain
cephalium shoots (Mauseth 1989; Mauseth and Kiesling
1997). Genes that control stomatal development for a leaf
epidermis are postulated to be active for the stem epidermis of cacti. This displaced developmental activity has
A
C
B
D
Figure 2.1. Dermal and cortical anatomical characteristics: (A) Polaskia chende, epidermal cells with irregular cell divisions and a two layer hypodermis; (B) Ariocarpus fissuratus, thick cuticle, papillose epidermal
cells and palisade parenchyma cells of the outer cortex; (C) Cephalocereus columna-trajani, epidermis with
crystals and thick-walled hypodermis; and (D) Myrtillocactus schenckii, rough cuticle, thick-walled hypodermis and mucilage cells in the outer cortex. Scale bars: A = 25 µm, B–D = 1 mm.
been called “homeosis” (Sattler 1988; Mauseth 1995a) and
may explain other aspects of cactus evolution.
Stomatal frequencies for cacti are low, 20 to 80 per
mm2, compared with leaves of C3 and C4 species, where
100 to 300 stomata per mm2 are common (Nobel 1994,
1999; Nobel and De la Barrera 2000). Within the Cactaceae, stomatal frequencies are highly variable (Table 2.1).
Some species of Opuntioideae and Cactoideae have frequencies that are as high as those for the lower leaf epidermis of species of Pereskia. The stomatal pore opening
for cacti tends to be large compared to other dicotyledons.
For instance, for Opuntia amyclaea, O. ficus-indica, O. joconostle, O. megacantha, O. robusta, and O. streptacantha,
the major axis of the pore varies from 33 to 62 µm (Conde
1975; Pimienta-Barrios et al. 1993), whereas pore major axes
are typically around 20 µm for non-cacti (Nobel 1999).
The pore length is oriented along the longitudinal axis of
the stem in Pereskioideae and Opuntioideae, but exhibits a
random orientation in most Cactoideae (Eggli 1984;
Butterfass 1987). In any case, the area of the open stomatal
pores for cacti tends to be less than for leaves of C3 and C4
species, reflecting the water-conserving use of CAM by
cacti (Nobel 1994, 1999; Chapter 4).
A hypodermis generally occurs under the epidermis
and usually consists of more than one cell layer in the
stem succulents of the Cactoideae and the Opuntioideae,
but is absent in Pereskioideae (Mauseth and Landrum
1997; Mauseth 1999). The number of layers of the hypodermis and the cell wall thicknesses may be related to the
rigidity and xeromorphy of the stems. The cell walls of
the hypodermis are often thickened with an accumulation
of pectin substances, and no hypodermis is lignified
(Gibson and Nobel 1986). For Cephalocereus columna-trajani (Fig. 2.1C) and Myrtillocactus schenckii (Fig. 2.1D),
the hypodermis is thick and consists of multiple layers.
For Opuntia spp. the hypodermis consists of a single layer
of cells, many of which contain solitary druses and a multilayered band of strong collenchymatous cells (Conde
1975; Pimienta-Barrios et al. 1993). Because of the druses
and its thickness, the hypodermis can affect the penetration of solar radiation to the underlying chlorenchyma
and represents a path through which gases must diffuse
(Parkhurst 1986; Darling 1989; Pimienta-Barrios et al.
1993).
Fundamental Tissue
The fundamental tissue, cortex and pith, carries out at least
two important functions related to xeric adaptations —
photosynthesis and water storage. For nearly all cacti, the
cortex is the most prominent region of the fundamental tis-
26
Terrazas Salgado and Mauseth
sue and is comprised of long-lived, thin-walled parenchyma
cells; even when the epidermis is replaced by periderm (outer
bark), the cortex is retained. In both Opuntioideae and
Cactoideae the pith tends to maintain its size with the age
of the stem and remains alive, which differs from many
other dicotyledonous species. The fundamental tissue also
includes specialized cells involved with secretion, such as
mucilage cells and laticifers. Also, cells in this tissue can produce the alkaloids, hormones, and other chemicals that contribute to metabolism (Mauseth 1984b; Nobel 1988, 1994).
Chlorenchyma
The outer cortex just below the hypodermis is commonly
characterized by multiple layers of cells arranged perpendicular to the stem epidermis and is called a palisade cortex,
which is made up of parenchyma cells (Figs. 2.1.A–D). The
palisade cortex is green and photosynthetic. The cells are radially elongated—generally two to eight times as long as
wide. About 13% of Pereskia stem tissue is intercellular air
space, which is approximately the same as for the palisade
parenchyma of its leaves (Sajeva and Mauseth 1991). In
most species of the Cactoideae, a layer of parenchyma with
large intercellular air spaces, one or two cells thick, occurs
between the hypodermis and the palisade cortex. In most
Cactaceae the photosynthetic tissue is in the stem, but in
Pereskia it occurs in leaf palisade and spongy parenchyma as
well as the stem cortex, which is narrow with small isodiametric cells (Sajeva and Mauseth 1991). The formation of
the palisade cortex in the stems of cacti is similar to that of
the palisade parenchyma in dicotyledonous leaves and may
similarly involve the breakdown or tearing of the middle
lamella accompanied by nonrandom separation of cells, another process of homeosis (Mauseth 1995a).
Inner Cortex
The inner cortex stores water that can be drawn upon during prolonged drought. The outermost layers of this region
contain some chlorophyll and presumably carry out some
photosynthesis, but the chlorophyll content is progressively lower and becomes absent for the innermost layers.
In the Cactoideae, about 9% of the volume of the inner
cortex is intercellular air space (Sajeva and Mauseth 1991),
but how easily water moves as liquid or vapor is not
known. Indeed, succulents undergo successive cycles of
filling and emptying their water-storage tissues. Collapsible
cortex, a special type of tissue that has flexible and apparently elastic walls, is found in Bolivicereus, Borzicactus,
Cleistocactus, Espostoa, Gymnocalycium, Haageocereus, Jasminocereus, Loxanthocereus, and many other taxa (Mauseth
1995b; Mauseth et al. 1998). These walls are thin and
TA B L E 2 . 1
Stomatal frequency for photosynthetic tissue in cacti
Subfamily
Frequency (number per mm2)
Pereskioideae
Opuntioideae
Cactoideae
leaf, 17–99 (51); stem, 2–20 (11)
leaf, 7–16 (12) (Pereskiopsis spp.); stem, 9–115 (80) (Opuntia spp.)
18–60 (31)
Data indicate the range, with the mean in parentheses, and are from Mauseth and Sajeva
(1991), Pimienta-Barrios et al. (1993), Nobel (1994), Arias (1996), and Nobel and De la
Barrera (2000).
unlignified and more flexible than those of the palisade
cells, readily allowing for volume changes of the cell. Five
to ten layers of such cells can occur in the innermost part
of the inner cortex, but not in the pith or medullary rays;
such cells occur even in regions of shoot tips that are less
than one year old, which have never experienced water
stress. When drought occurs and the plant’s rate of water
intake falls below its rate of water loss, the flexible walls
permit the collapsible parenchyma cells to release water
while the less flexible walls of the palisade cortex cells retain water (Goldstein et al. 1991). Consequently, water
from the collapsible water-storage tissue replaces water lost
from the photosynthetic tissue.
Most of the water in the stems of cacti is in the inner
cortex. The cells have large vacuoles and can lose four times
more water than is lost from the smaller cells of the
chlorenchyma (Nobel 1994). For the barrel cactus Ferocactus
acanthodes, solutes decrease in the inner cortex and pith (either by polymerization or transport out of the cells), lowering the osmotic pressure and thereby favoring the redistribution of water to the chlorenchyma (Barcikowski and
Nobel 1984). That is, water diffuses from this storage region
into regions of higher osmotic pressure in the chlorenchyma. Similar processes occur for the platyopuntia Opuntia
basilaris, except that for this species most water storage occurs in the pith (Gibson and Nobel 1986). By maintaining
higher water content in the chlorenchyma, nocturnal opening of stomata and net CO2 uptake are allowed to continue for a longer period than would be the case if all the tissue were to dry at the same rate (Chapter 4).
Pith
One difference of many Cactaceae in relation to other dicotyledons is the presence of a radially thick pith in the center of the stem (Bailey 1962, 1963a,b; Gibson and Horak
1978; Mauseth 1989). This pith occurs within the stele and
generally occupies a small fraction of the stem volume
(Mauseth 1993a), except for platyopuntias (Nobel 1988).
The cells are generally thin walled, isodiametric, and living;
they act as a water reservoir, often contain starch grains, and
may store a variety of allelochemicals. In large-stemmed
Cactoideae, the pith may also contain medullary bundles,
which facilitate radial water movement (Mauseth 1993a).
The short cylindrical, globose, or disc-shaped cacti of the
Cacteae, Echinocereae, and Notocacteae tribes lack medullary bundles in their relatively small piths.
Mucilage Cells, Laticifers, and Sclereids
The stem tissues of cacti often contain large quantities of
the complex carbohydrate mucilage, which is hydrophilic
and affects water relations (Gibson and Nobel 1986;
Goldstein and Nobel 1991; Nobel et al. 1992). Mucilage
cells (idioblasts that produce mucilage; Fig. 2.2A), which
lack chloroplasts and starch grains, were first described by
Lauterbach in 1889 (Mauseth 1980). Lloyd (1919) pointed
out that although hydrated intracellular mucilage crowds
the protoplast, the cell mucilage can aid in cell growth because it imbibes water. The mucilage content and composition in a mucilage cell vary with time of year and species.
For older stems or during extensive drought, the mucilage
cells may contain crystals (Fig. 2.2B). Both Pereskia and
Maihuenia have mucilage cells, but they are more abundant in Maihuenia, for which the very large mucilage cells
often compose over half of the leaf volume (Mauseth
1999). Mucilage cells are also abundant in the Cactoideae
and Opuntioideae, generally occurring in the inner cortex
and pith. Sometimes mucilage cells can occur just below
the hypodermis within the palisade parenchyma, as for
Echinocereus sciurus (Fig. 2.2A). Mucilage cells vary from
about 40 µm to over 1.0 mm in diameter and often resemble cavities in the inner cortex, as for Stenocereus
thurberi and S. martinezii (Gibson 1990; Terrazas and
Loza-Cornejo 2002). Other mucilage-containing cavities
are present in the pith of several species of Ariocarpus
Shoot Anatomy and Morphology
27
A
B
C
D
Figure 2.2. Mucilage cells and mineral inclusions: (A) Echinocereus sciurus, abundant mucilage cells; (B) Wilcoxia
poselgeri, crystal in a mucilage cell of the cortical region; (C) Escontria chiotilla, sphaerocrystal in the cortical
region; and (D) Stenocereus gummosus, silica grains in the epidermal cells. Scale bars: A = 1 mm, B = 100 µm,
C–D = 20 µm.
(Anderson 1960, 1961), and a unique network of anastomosed canals containing cells essentially filled with mucilage occur in Nopalea spp. (Mauseth 1980).Fgiure2.neahrere:
In 1889 Lauterbach reported laticifers (idioblasts that
produce latex) for Coryphantha, Leuchtenbergia, and
Mammillaria, but they were not extensively described until
1978 (Mauseth 1978a,b). The composition and abundance
of latex varies among species. In the Mammillaria section,
laticifers are abundant and referred to as “milky,” those in
section Subhydrochylus are referred to as “semi-milky,”
whereas those in section Hydrochylus lack laticifers altogether (Mauseth 1978b). Laticifers in Mammillaria differ
from those of other plant families and are unique among
cacti (Mauseth 1978a). In section Mammillaria, laticifers
occur in the pith, throughout the cortex, the basal half of
the tubercles (modified leaf bases), and even the entire tubercle, where they laterally branch to the hypodermis.
Laticifers in section Subhydrochylus form only in the outermost cortex and the bases of the tubercles. For plants in
sections Mammillaria and Subhydrochylus, laticifers develop by rapid cell division of wide groups of parenchyma
cells. At maturity both have a central lumen lined by an epithelium one to several cell layers thick (Wittler and
Mauseth 1984). However, laticifers in section Subhydrochylus resemble the ancestral condition because they are
more irregular in shape, lumen development, and epithelium form than those in section Mammillaria.
Two distinct forms of idioblastic sclereids (dead,
lignified cells) occur in the stems of certain columnar cacti,
such as Eulychnia spp., Pachycereus pringlei, and Stetsonia
coryne (Gibson and Nobel 1986; Nyffeler et al. 1997). One
type of sclerenchyma cell is slender and distinctly elongated and occurs in the outer cortex. The other form is globular or subglobular and occurs in the inner cortex and the
pith. Sclereids provide mechanical strength due to their
thickened, lignified cell walls and aid in lessening collapse
of the cortex during drought. Other columnar cacti do not
possess idioblastic sclereids, but instead have a cortex with
many large mucilage cells, indicating different strategies for
adaptation to arid environments.
Mineral Inclusions
Cacti can accumulate enormous quantities of calcium oxalate. For example, up to 85% of the dry weight of
Cephalocereus senilis can be Ca oxalate (Cheavin 1938). As
a result, most cacti have Ca oxalate crystals in their stems,
which may be prismatic (sharp angles), druses (star-like),
and, rarely, acicular (needles). Crystals are formed in the
central vacuole via a complicated precipitation process,
which may be an end-product of metabolism and/or may
serve as a means of removing excess Ca from the cells
(Franceschi and Horner 1980). Plants grown using solutions high in Ca often form more crystals than control
plants. In addition to the insoluble Ca salts, many plants
contain high concentrations of soluble oxalate, which can
affect osmotic pressure (and thus turgor and volume regulation) in the cells. A major function attributed to Ca oxalate crystals is that of protection against foraging animals.
The irritation and burning sensations of the mouth caused
by eating crystal-containing plants is well known, and large
quantities of oxalate can be fatal.
Different forms of Ca oxalate and other chemicals are
involved in crystal formation. Using X-ray diffraction,
Rivera (1973) found druses with the monohydrate form of
Ca oxalate in Opuntia imbricata and the dihydrate form in
Echinocactus horizonthalonius, E. intertextus, and Escobaria
tuberculosa. The dihydrate form also occurs in prismatic
crystals. Leaves of Pereskiopsis contain both Ca oxalate and
Ca malate crystals (Bailey 1966). Members of Cactoideae
may contain sphaerocrystals (spherical; Fig. 2.2C), the
composition of which is unknown, and their form differs
from other crystal types (Metcalfe and Chalk 1950; LozaCornejo and Terrazas 1996). Some species contain only one
crystal type, whereas others may have two or more types,
even in adjacent cells (Gibson 1973). Crystals are common
in secondary xylem and may be deposited in axial or radial parenchyma (Gibson 1973; Mauseth 1996, 1999; Terrazas
and Loza-Cornejo 2001).
The occurrence of crystals in the epidermal cells often
has taxonomic value (Chapter 1), but their occurrence in
the cortex and pith is more variable and therefore has low
taxonomic value. For example, Cephalocereus and Neobuxbaumia are the only members of tribe Pachycereeae with
prismatic crystals in their epidermal cells (Gibson and
Horak 1978; Terrazas and Loza-Cornejo 2001). Members of
tribe Hylocereeae contain acicular crystals in their epidermal cells (Gibson and Nobel 1986; Mauseth et al. 1998;
Loza-Cornejo and Terrazas 2001), while other species have
distinctive druses in their hypodermal cells (PimientaBarrios et al. 1993; Mauseth 1996; Loza-Cornejo and
Terrazas 2001).
Silica bodies are also prominent in the epidermal and
hypodermal cells of certain cacti and are valuable taxonomically (Fig. 2.2D). Their occurrence is diagnostic for all
members of Stenocereus and Rathbunia (Gibson and Horak
1978). Silica grains also occur in the epidermal cells of other
Cactoideae members (Loza-Cornejo and Terrazas 2001) and
in the ray cells of Pachycereus weberi (Terrazas and LozaCornejo 2002), but they have not been observed in the
Pereskioideae or Opuntioideae (Gibson and Nobel 1986).
Shoot Anatomy and Morphology
29
Vascular Tissue
Vascular tissue, which is involved in movement of substances in plants, is highly specialized in cacti. The main
and largest vascular bundles occur in the stele, which lies
between the inner cortex and the pith. The two tissue types
are the xylem, which serves to move water as well as dissolved nutrients, and the phloem, which distributes photosynthetic products and other organic molecules. Primary
xylem and phloem develop during the initial stages of
growth, and, periodically, secondary tissues subsequently
develop. Vascular tissue also occurs in the cortex (cortical
bundles) and the pith (medullary bundles).
Cortical and Medullary Bundles
Cortical bundles, which are absent in Pereskioideae and
Opuntioideae, generally occur throughout the cortex but
do not extend to the hypodermis in members of the
Cactoideae (Mauseth 1995a, 1999a). They occur in all directions and change direction frequently. Cortical bundles
are collateral and contain primary and secondary xylem
and phloem. Secondary phloem accumulates at a higher
rate than secondary xylem, which may or may not increase
with stem age (Mauseth and Sajeva 1992). For example, for
Mammillaria parkinsonii and Pediocactus simpsonii, older
bundles have much more xylem than younger ones. In
some species, cortical bundles contain phloem fibers that
differentiate adjacent to the conducting cells of the
phloem, such as for species of Acanthocereus (Mauseth et al.
1998), Bergerocactus emoryi (Terrazas and Loza-Cornejo
2002), and Selenicereus inermis (Mauseth and Sajeva 1992).
Xylary fibers in cortical bundles are rare but occur in
Pilosocereus mortensenii (Mauseth and Sajeva 1992).
Cortical bundles appear to be involved in three processes (Mauseth and Sajeva 1992): (1) transporting photosynthate from the outer, chlorophyllous palisade cortex to the
stele; (2) transporting sugars to and from storage cells in the
inner, nonphotosynthetic cortex; and (3) transporting water
throughout the cortex. Phloem in cortical bundles is probably involved in sugar transfer when the cortex acts as a
starch storage tissue. Cortical bundles accumulate phloem as
they age, indicating the continued production of phloem
and presumably greater translocation of sugars, which probably cannot be transported from the outer cortex to the stele
rapidly enough by diffusion alone (Nobel 1999). Cortical
bundles resemble leaf veins in spacing, structure, presence of
narrow conducting cells, and solute distribution. Mauseth
and Sajeva (1992) conclude that cortical bundles, whose life
span in cacti is long compared to the leaf veins in most dicotyledons, have arisen independently in the Cactaceae.
30
Terrazas Salgado and Mauseth
Medullary bundles, which are similar in size to cortical bundles, are initiated close to the apical meristem, may
have secondary xylem and phloem, and occur only in subfamily Cactiodeae (Boke 1954; Bailey 1962; Gibson and
Horak 1978; Mauseth and Ross 1988; Mauseth 1993a,
1999). Medullary bundles are closely spaced when initiated near the shoot tip, but as the shoots continue growing,
the pith expands and medullary bundles are pushed to a
wider spacing, with very low densities in the older trunks
(Mauseth 1993a). A few species (e.g., Brachycereus nesioticus, Jasminocereus thouarsii, Monvillea maritima) are distinctive because primary phloem fibers differentiate adjacent to the medullary bundle phloem. Xylary fibers in
medullary bundles are rare but present in Jasminocereus
thouarsii. Medullary bundles are interconnected with stele
bundles, and, in several cases, they completely transverse
the broad primary rays and are interconnected with the
cortical bundles. Although medullary bundles appear to be
relictually absent in the family, they may have originated
during the early stages of the evolution of Cactoideae. In
fact, a secondary loss of medullary bundles may have occurred in several species of Cactoideae that have a narrow
pith and a relatively broad cortex (tribe Cacteae, some
Notocacteae, and some Echinocereae; Boke 1956, 1957;
Gibson and Horak 1978; Mauseth 1993a; Loza-Cornejo
and Terrazas 1996; Mauseth et al. 1998).
Medullary bundles should permit a cactus to translocate water and starch to and from a broad pith. Because
they continue to produce phloem throughout the life of
the plant and starch is abundant in many pith sections,
transport of carbohydrates is an important role for
medullary bundles. Water transport throughout the pith
may also be important, but may proceed slowly.
Xylem
The water-conducting conduits of the shoots of cacti are
vessels occurring either solitary or in small clusters of 2 to
10 vessels (Gibson 1973, 1978). Vessels are narrow, usually
ranging from 10 to 60 µm in diameter, are dead at maturity, and consist of tubes of primary and secondary wall
(Nobel 1999). The widest vessels occur in the primitive
genus Pereskia and the narrowest in species of the epiphytic
genus Rhipsalis (Gibson and Nobel 1986). The vessel elements have simple perforation plates, a highly derived trait
that facilitates fluid movement along a vessel (Nobel
1999). Also present are libriform fibers (phloem-like fibers;
Fig. 2.3A) and wide-band tracheids (often referred to as
vascular tracheids; Fig. 2.3B). Wide-band tracheids are imperforate, broadly fusiform cells with either helical or annular thickenings; the thickenings project deeply into the
A
B
C
D
Figure 2.3. Secondary xylem and phloem: (A) Peniocereus striatus, distinctive short living fibers; (B) Echinocereus
schmollii, abundant wide-band tracheids; (C) Pachycereus pringlei, sclereid in the collapsed phloem and dilatated
rays; and (D) Wilcoxia poselgeri, phloem parenchyma cells with tannins between noncollapsed phloem and cortical cells. Scale bars: A, B, D = 20 µm, C = 1 mm.
lumen (Gibson 1973, 1977, 1978; Mauseth et al. 1995;
Mauseth and Plemons 1995). Because wide-band tracheids
lack perforations, they play little role in rapid water conduction between adjacent cells. Rays in cacti are extremely variable in width and length but are generally wider than
in typical dicotyledons, an adaptation that facilitates water
storage (Gibson and Nobel 1986). Rays also function in the
lateral movement of water, as evidenced by the perforations
in the ray cell walls for some cacti (Terrazas 2000). In addition to wide rays, other special features of cactus xylem
for water storage are that the fiber cell walls are thin, which
increases lumen volume, and that vessels and paratracheal
parenchyma constitute a large fraction of the volume of the
axial system (Mauseth 1993b).Fgiure23n.eahrere:
Phloem
Primary and secondary phloem in Cactaceae varies among
species but is generally composed of sieve tube members,
companion cells, and axial and radial parenchyma. In most
cacti with elongate stems, the young vascular bundles of
the stele may have a cap of primary phloem fibers, which
are thick-walled, septate, and nucleated. The size of the
phloem fiber cap varies among species but does not appear
to be an indicator of phylogenetic relatedness (Mauseth
1996). As secondary phloem accumulates, the older, more
peripheral phloem collapses and dies, while the younger
non-collapsed phloem near vascular cambium consists of
living, functioning cells. The older sieve tube members and
companion cells collapse into dark-staining masses that
may be oriented either tangentially or radially to the stele.
For some species, parenchyma cells may redifferentiate
into sclereids (Fig. 2.3C), as for Pereskia and some members
of the tribes Browningieae, Cereeae, Echinocereeae, and
Pachycereeae (Mauseth 1996; Mauseth et al. 1998; Terrazas
and Loza-Cornejo 2002). Other species — usually those
with short globose shoots — develop neither primary
phloem fibers nor sclereids associated with the collapsed
phloem (Gibson and Nobel 1986; Loza-Cornejo and
Terrazas 1996; Mauseth et al. 1998; Mauseth 1999a). Species
of Wilcoxia have specialized parenchyma cells that contain
abundant tannins (Fig. 2.3D; Loza-Cornejo and Terrazas
1996). The lack of a phloem fiber cap generally is associated with the occurrence of wide-band tracheids in the secondary xylem (Mauseth et al. 1998). Sclereids or fibers do
not form associated to phloem in cephalia of Leptocereus,
Melocactus, and Neoabbottia (Mauseth 1989; Mauseth et al.
1998). Phloem rays expand as secondary growth occurs.
Phloem rays have living thin-walled parenchyma cells and
may contain abundant mucilage cells or druses. They connect with the primary or secondary xylem rays and may
32
Terrazas Salgado and Mauseth
contribute to the distribution of photosynthetic products
and other organic molecules.
Wood
Various types of wood occur in the Cactaceae (Gibson
and Nobel 1986; Mauseth and Plemons 1995; Mauseth
and Plemons-Rodriguez 1998; Arnold and Mauseth
1999). They can be classified according to the type of matrix they have (fibrous, parenchymatous, or wide-band
tracheids) or according to the uniformity of wood development (monomorphic or polymorphic). The axial portion of fibrous wood, i.e., the portion produced by
fusiform initials and whose cells are elongate parallel to
the axis of the shoot, consists of vessels embedded in a
matrix of living libriform fibers. Typically, the fibers constitute over half of the wood volume, giving it strength,
flexibility, and resistance to breaking. Fibrous wood is
found in all cacti that are too large to hold themselves up
by turgor pressure—all members of Pereskia, aborescent
Opuntiodeae, Armatocereus, Pachycereus, and other large
cacti (Gibson 1973; Mauseth 1992; Mauseth and Landrum 1997; Terrazes 2001). Considerable variation occurs
in the anatomical details of the fibrous wood. Most
species have fibers that lack septae, whereas in other
species all or most of the fibers are septate, with a single
septum of primary cell wall. Vessels in fibrous wood are
generally wider and less frequent than in other types of
wood (Table 2.2). The added strength of fibers may allow
for larger vessels, thus increasing water movement per
vessel because volume flux is dependent on the vessel radius raised to the fourth power (Nobel 1999).
Parenchymatous wood has a matrix of unlignified
parenchyma cells with thin primary cell walls. Parenchymatous wood is too soft to provide significant support
to the shoot and is found in cacti whose shoots are
procumbent, globose, or immersed in the soil. Within the
parenchymatous matrix, vessels are smaller in diameter
than for vessels in fibrous wood of taller or older plants
(Tables 2.2 and 2.3). The vessels are either solitary or occur
in clusters, as in fibrous wood, but they have a tendency to
form large clusters of up to 100 vessels and thus are more
frequent than for the fibrous wood of larger growth forms
(Table 2.2 and 2.3). Rays in parenchymatous wood tend to
be large and purely parenchymatous and the cells lack secondary walls. Parenchymatous wood tends to be formed in
small amounts, with only a few millimeters from pith to
vascular cambium. Even though some shoots can have
massive amounts of cortex or pith parenchyma, they never
have massive amounts of parenchymatous wood. Vessels
within parenchymatous wood are in intimate contact
:erh
eran
e2.2eT
lba
TA B L E 2 . 2
Mean vessel diameter and frequencies for different types of wood present in
the Cactaceae
Subfamily
Vessel diameter (µm)
Vessels/mm2
59
—
—
66
—
—
58
—
51
—
—
—
38
26
27
168
554
196
Pereskioidae
Fibrous
Parenchymatous
Wide-band tracheids
Opuntioideae
Fibrous
Parenchymatous
Wide-band tracheids
Cactoideae
Fibrous
Parenchymatous
Wide-band tracheids
References: Gibson (1973, 1977a,b, 1978) and Mauseth and PlemonsRodriguez (1998).
TA B L E 2 . 3
Mean values for four wood anatomical features associated with life-form in subfamilies of the Cactaceae
Subfamily
Pereskioideae
Tall plants
Intermediate height
Short, globose
Opuntioideae
Tall plants
Intermediate height
Short, globose
Cactoideae
Tall plants
Intermediate height
Short, globose
Vessel diameter
(µm)
Vessels/mm2
Vessel element
length (mm)
Fiber length
(mm)
59
—
30
66
—
689
—
—
—
878
—
—
75
56
44
10
23
—
234
164
140
704
543
463
44
33
27
131
296
336
359
291
182
878
683
369
Data are from Gibson (1973, 1977a,b, 1978), Loza-Cornejo and Terrazas (1996), Mauseth and Plemons-Rodriguez (1998),
Bobich and Nobel (2001), and Herrera et al. (2001).
with stored water, which can help prevent embolisms (or
help refill cavitated ones; Mauseth 1993a).Tabe2l3n.eahrere:
Wide-band tracheid wood appears to be highly adaptive
for plants in extremely xeric habitats. It typically contains
vessels, but in some species vessels exist only as a component of earlywood. Such vessels have essentially the same
diameter as for vessels in parenchymatous wood for
Cactioideae, but are much less frequent (Table 2.2). Cactus
wood of any type virtually never has annual growth rings.
The most clear-cut examples of growth rings are restricted
to wide-band tracheid wood of North American small cacti
(Ferocactus, Echinocactus, and relatives), but not to the South
American ones (Gymnocalycium, Echinopsis, and others). In
wood that has annual rings, the presence of a vessel-rich earlywood combined with a latewood of such wide-band tracheids makes growth rings visible to the naked eye. In
South American taxa with wide-band tracheid wood, vessels and apotracheal parenchyma are mixed in with late-
Shoot Anatomy and Morphology
33
wood; consequently, earlywood and latewood are difficult
to distinguish, and no growth rings can be detected. Such
wood of North American taxa also differs from that of
South American taxa with respect to rays. Rows of wideband tracheids occur next to well defined rows of ray parenchyma cells for North American taxa, whereas in South
American taxa, a series of parenchyma cells that appears to
be a ray will be interrupted by a single wide-band tracheid.
Existing studies on wide-band tracheids are mainly
on Cactoideae and Maihuenia in the Pereskioideae/
Maihuenioideae. In Opuntioideae, wide-band tracheids
occur mostly in the rays, and the wood consists of narrow
axial masses. Also for the Opuntioideae, wide-band tracheids, which can be found in either parenchymatous or
fibrous wood, are usually in the ground tissue next to the
protoxylem and occur as both axial and ray cells in the
wood, being larger and more numerous in ray cells that are
adjacent to the axial regions than they are in the axial regions (Gibson 1973, 1978). In platyopuntias, wide-band
tracheids are often arranged in radial files of three or more,
and the annularly thickened secondary cell walls of adjacent cells often alternate, giving them an interlocking appearance (Bobich and Nobel 2001).
Several cactus species have dimorphic or trimorphic
wood, meaning that when young, they produce one type
of wood, but they produce a distinctly different wood type
as they mature (Mauseth and Plemons 1995; Mauseth and
Plemons-Rodriguez 1998). A common dimorphism is
one in which a plant produces wide-band tracheid wood
when the plant is young and small enough that turgor
pressure will support the shoot. As the plant becomes
taller and heavier, a stronger wood is needed, and fibrous
wood is produced. When viewing a transverse section of
this wood dimorphism, wide-band tracheid wood will
occur near the center of the stem and fibrous wood will
occur near the vascular cambium. A second, rather common type is one in which vine-like or hemiepiphytic cacti
with elongate shoots clamber through the branches of
trees, allowing the cactus shoots to rest on the tree’s
branches. These will often have fibrous wood in the center and parenchymatous wood near the vascular cambium,
reflecting the growth of these cacti, which initially have
upright self-supporting branches that eventually lean on
tree branches for support.
A third example of dimorphic wood involves Melocactus, a genus in which adult plants produce a cephalium
(a shoot terminus producing flowers and densely covered
by spines or trichomes). While growing as a juvenile, nonflowering plant, the shoot makes fibrous wood, but once
it becomes old enough to flower, its morphology changes
34
Terrazas Salgado and Mauseth
and switches to making parenchymatous wood. Thus, the
lower regions of a Melocactus has both types of wood,
whereas the cephalium has only the adult-phase, parenchymatous wood. The stimulus that triggers the conversion affects the entire plant simultaneously. For other
species, the conversion appears to be related to the age of
the cambium at each particular area. For example, the basal
part of a plant may have converted to the second type of
wood, whereas the upper part of the stem is too young, so
the stem is producing two types of wood at the same time.
A particularly intriguing aspect of dimorphic wood is
that, when wide-band tracheid wood is present, it is always
the first type of wood formed. Dimorphic wide-band tracheid-to-fibrous wood has originated several times, with
the wide-band tracheid wood being a juvenile feature produced for only a few years. Small cacti with short life spans
generally have the greatest fractions of wide-band tracheid
wood in their stems when compared with taller, longerlived forms. In fact, some of the smallest cacti never produce fibrous wood, having either wide-band tracheid
wood or parenchymatous wood throughout their lifespan.
For species that normally have small adults with wide-band
tracheid wood, exceptionally large individuals may produce fibrous wood once they become old. In taxa such as
Epithelantha or Frailea, plants always remain too small, but
individuals in taxa such as Echinopsis, Soehrensia,
Ferocactus, Echinocactus, and Echinocereus may actually
produce fibrous wood.
Morphology
Fundamentally, the morphology of cacti is like that of other
seed plants: the shoot consists of internodes, nodes where
leaves are attached (although leaves are little more than leaf
primordia in all Cactoideae), and axillary buds (the spineproducing areoles). The bud scales and leaves of axillary
buds have become the signature spines of cacti. An axillary
bud produces spines as soon as it is initiated by the shoot
apical meristem; afterward it may produce a flower and/or
a vegetative branch. Cactus stems exhibit short shoot–long
shoot architecture. The areoles with their spine-leaves are
short shoots, whereas the body of a cactus is a long shoot
with highly reduced green leaves (the long-shoot leaves are
not reduced in Pereskioideae and Opuntioideae).
Cactus morphology varies from ordinary trees (Pereskia,
Fig. 2.4A) to large arborescent Opuntioids (Opuntia, Fig.
2.4B) and columnar cacti (Carnegiea, Fig. 2.4C; Pachycereus) to scrambling succulents (Acanthocereus; Harrisia;
Rathbunia, Fig. 2.4D) to short-columnar and sparsely
branched shrubs (Echinocereus, Haageocereus) to unbranched globose forms (Eriosyce; Mammillaria, Fig. 2.4E)
A
D
C
B
E
F
Figure 2.4. Morphology of various cactus species: (A) Pereskia grandifolia; (B) Opuntia echios var. gigantea; (C) Carnegiea gigantea; (D) Rathbunia alamosensis; (E) Mammillaria magnimamma; and (F) Pterocactus tuberosus. Photographs are courtesy of Edward G. Bobich (A, C–F) and
Park S. Nobel (B).
to geophytes (Pterocactus, Fig. 2.4F). Reduction in the
number of branches with increased succulence is noteworthy and perhaps is related to the increased weight due
to succulence—a cactus with branches as thick as those of
a Pachycereus or a Trichocereus simply cannot have as many
branches as does a Pereskia in which the branches are slender and relatively light. A correlated change accompanying
reduced branching is the reduced number of shoot apical
meristems — an unbranched Cephalocereus, Ferocactus, or
Soehrensia has just one single apical meristem with which
to construct its entire body. Certainly this has significant
consequences. Branching within a taxon is often affected
by environmental factors; eg., greater annual precipitation
is associated with more infrequent branching in the tribe
Pachycereeae (Cornejo and Simpson 1997).Fgiure24.neahrere:
A significant change in morphology with age involves
juvenile/adult heteromorphy. For many cacti, mature
flowering adults resemble sexually immature plants.
However, for genera with cephalia, such as those in Backebergia (Fig. 2.5A), Discocactus, or Melocactus (Fig. 2.5B),
older reproductive plants and younger plants do not resemble each other. The juvenile body looks like an ordinary globose cactus with prominent ribs, a green body,
widely spaced axillary buds, and larger spines. The cephalium—which is a continuation of the stem, produced by the
same apical meristem (Niklas and Mauseth 1981) — lacks
ribs, has very closely spaced axillary buds, and produces an
abundance of trichomes and short spines that hide the
stem surface. The cephalium epidermis converts to cork
cambium, so the surface of a cephalium is bark-covered
Shoot Anatomy and Morphology
35
A
B
C
Figure 2.5. Cephalia: (A) apical cephalia for Backebergia militaris; (B) apical cephalium for Melocactus peruvianus; and (C) lateral cephalium for
Espostoa melanostele. Photographs are courtesy of Arthur C. Gibson.
and brown (Mauseth 1989). In most cacti, each axillary
bud (areole) can produce only a single flower. For cephalia,
axillary buds are close together and the stems are narrow,
which reduces the expense of producing each new bud
(and thus each new flower). Also, because flower production is the only apparent role for cephalia, the buds can be
protected with the tight mass of spines and trichomes—a
mass so dense that not only are insects prevented from
reaching the buds, but so is light (and hence no photosynthesis). Apical cephalia prevent the plant from producing any new photosynthetic tissues, so the photosynthetic
capacity decreases with age because the existing photosynthetic tissues become less efficient over time.Fgiure25n.eahrere:
Plants with lateral cephalia (Fig. 2.5C) are able to produce both new chlorophyllous tissue and closely spaced,
well-protected axillary buds and thus flowers. These plants
grow as juveniles for several years; when old enough, several ribs on one side of the body undergo a transition to
adult morphology and their axillary buds are able to produce flowers. In addition, they also produce copious trichomes and spines, so the flowers are well protected, and
the phyllotaxy of the affected ribs differs from that of the
rest of the stem, with buds much closer together. The
plants continue to grow like this, with one side being
adult, and the other juvenile. Lateral cephalia occur for
Espostoa (Fig. 2.5C), Espostoopsis, Micranthocereus, Thrixanthocereus, Vatricania, and others (Mauseth 1999b). In
Cephalocereus, the cephalium is initially lateral, but as the
36
Terrazas Salgado and Mauseth
plants continue to grow, occasionally adjacent ribs are recruited to the cephalium so the cephalium eventually becomes terminal.
For Neoraimondia and Neocardenasia, axillary buds can
produce several flowers simultaneously and are active year
after year (Mauseth and Kiesling 1997). With each flowering, a short shoot stem is produced, but after many years
the areoles develop into shoots up to 10 cm long and occasionally even branch. In effect, the short shoots of these
genera are a type of cephalium. Members of the genus
Pilosocereus produce a pseudocephalium. Again, only a few
ribs produce flowers as well as copious, long trichomes, but
the phyllotaxy is not altered nor is the interior anatomy
changed. Once the trichomes break off, the axillary buds
that have flowered are more or less indistinguishable from
those that never flowered.
Biomechanics
The biomechanics of plant organs are affected by both
anatomy and morphology. For all cacti, both wood composition and accumulation affect stem strength (MolinaFreaner et al. 1998; Niklas 2000; Bobich and Nobel 2001).
For instance, Carnegiea gigantea (saguaro) stems appear to
become stiffer over time — possibly due to increases in
lignified tissue in the wood—without having appreciable
increases in stem diameter, thus allowing plants to become
disproportionately slender as height increases (Niklas and
Buchman 1994). The amount of lignification of wood,
rather than the fiber length or cell wall thickness, appears
to be responsible the for increases in strength with age for
the wood of Pachycereus pringlei (Niklas et al. 2000).
Interestingly, increases in wood strength from the apex to
the base for a P. pringlei stem is non-linear (Niklas et al.
1999) as a result of the stem wood having less lignin at the
base than for regions more than 1 m above the ground
(Niklas et al. 2000). This apparently lessens shear stress at
the cellular level near the base and also allows for the dissipation of tensile and compressive stresses, thereby decreasing the probability of stem failure at the base.
Increases in the frequencies of cells with secondary cell
walls, especially libriform fibers, also appears to have a positive correlation with resistance to bending stresses for the
junctions between two stem segments (cladodes) for arborescent and frutescent platyopuntias (Bobich and Nobel
2001).
Increases in strength for the junctions of cladodes also
correlates with the section modulus of the stem, which increases with wood accumulation (Bobich and Nobel 2001).
The same correlation of stem strength with stem diameter
is observed in columnar cacti (Molina-Fraener et al. 1998).
In fact, slender columnar cacti like Stenocereus gummosus
may often exceed the critical height allowed by their stem
diameter, thus leading to the mechanical failure of the
stems. For a threefold increase in length for the cladodes of
the arborescent platyopuntia, Opuntia ficus-indica, the angular deflection under their own weight approximately
doubles, reflecting flexure of both the cladode and the
cladode-cladode junction (Nobel and Meyer 1991).
Conclusions and Future Prospects
Because of adaptations to xeric conditions and the presence
of less derived woods in Pereskioideae, the Cactaceae is one
of the most interesting families both anatomically and
morphologically. Increases in water-storage tissue, especially in the cortex and wood, thickened cuticles, and the
presence of a hypodermis are all well-known xeromorphic
adaptations. However, there is much more to be gained by
further anatomical and morphological research. For instance, what causes the presence of wide-band tracheids in
wood is relatively unknown. Furthermore, the role of these
cells is not fully understood. Also, the “annual growth
rings” seen for some cacti need further study; most such
anatomical features probably reflect extremely wet versus
extremely dry conditions seasonally. A careful study is necessary of the vascular cambium to distinguish between ray
and fusiform initials. Finally, the relationship between
anatomy and morphology, especially in structural terms,
has yet to be investigated for a variety of growth forms,
e.g., epiphytes, vine cacti, and shrubby forms. There is indeed much more to be known about the anatomy and
morphology that lead to the various forms of cacti.
Acknowledgment
The authors gratefully thank Edward Bobich and Park
Nobel for substantial contributions to the writing of this
chapter.
Literature Cited
Anderson, E. F. 1960. A revision of Ariocarpus (Cactaceae).
I. The status of the proposed genus Roseocactus. American Journal of Botany 47: 582–589.
Anderson, E. F. 1961. A study of the proposed genus
Roseocactus. Cactus and Succulent Journal (U.S.) 33:
122–127.
Arias, S. 1996. Revisión taxonómica del género Pereskiopsis
Britton et Rose. Master’s Thesis, Universidad Autónoma
de México, Mexico City.
Arnold, D. H., and J. D. Mauseth. 1999. Effects of environmental factors on development of wood. American
Journal of Botany 86: 367–371.
Bailey, I. W. 1962. Comparative anatomy of the leafbearing Cactaceae. VI. The xylem of Pereskia sacharosa
and Pereskia aculeata. Journal of the Arnold Arboretum
43: 376–383.
Bailey, I. W. 1963a. Comparative anatomy of the leaf-bearing Cactaceae. VIII. The xylem of pereskias from
southern Mexico and Central America. Journal of the
Arnold Arboretum 44: 211–216.
Bailey, I. W. 1963b. Comparative anatomy of the leaf-bearing Cactaceae. VII. The xylem of pereskias from Peru
and Bolivia. Journal of the Arnold Arboretum 44: 127–
133.
Bailey, I. W. 1966. Comparative anatomy of the leaf-bearing Cactaceae. XVI. The development of water-soluble
crystals in dehydrated leaves of Pereskiopsis. Journal of
the Arnold Arboretum 47: 273–283.
Barcikowski, W., and P. S. Nobel. 1984. Water relations of
cacti during desiccation: Distribution of water in tissues. Botanical Gazette 145: 110–115.
Bobich, E. G., and P. S. Nobel. 2001. Biomechanics and
anatomy of cladode junctions for two Opuntia
(Cactaceae) species and their hybrid. American Journal
of Botany 88: 391–400.
Boke, N. H. 1954. Organogenesis of the vegetative shoot in
Pereskia. American Journal of Botany 41: 619–637.
Shoot Anatomy and Morphology
37
Boke, N. H. 1956. Developmental anatomy and the validity of the genus Bartschella. American Journal of Botany
43: 819–827.
Boke, N. H. 1957. Structure and development of the
shoot in Toumeya. American Journal of Botany 44: 888–
896.
Boke, N. H. 1959. Endomorphic and ectomorphic characters in Pelecyphora and Encephalocarpus. American
Journal of Botany 46: 197–209.
Boosfeld, A. 1920. Beiträge zur vergleichenden Anatomie
stammsukkulenter Pflanzen. Beihungen der Botanisches
Centralblatt 37: 217–258.
Butterfass, Th. 1987. The transverse orientation of stomata. The Botanical Review 53: 415–441.
Cheavin, W. H. S. 1938. The crystals and cytoliths found in
plant cells. Part. I. Crystals. Microscope 2: 155–158.
Conde, L. F. 1975. Anatomical comparisons of five species
of Opuntia (Cactaceae). Annals of the Missouri Botanical Garden 62: 425–473.
Cornejo, D. O., and B. B. Simpson. 1997. Analysis of form
and function in North American columnar cacti (tribe
Pachycereeae). American Journal of Botany 84: 1482–
1501.
Darling, M. S. 1989. Epidermis and hypodermis of the
saguaro cactus (Cereus giganteus): anatomy and spectral
properties. American Journal of Botany 76: 1698–1706.
Eggli, U. 1984. Stomatal types. Plant Systematics and Evolution 146: 197–214.
Fahn, A. 1986. Structural and functional properties of trichomes of xeromorphic leaves. Annals of Botany 57:
631–637.
Franceschi, V. R., and H. T. Horner Jr. 1980. Calcium oxalate crystals in plants. Botanical Review 46: 361–427.
Gibson, A. C. 1973. Comparative anatomy of secondary
xylem in Cactoideae (Cactaceae). Biotropica 5: 29–65.
Gibson, A. C. 1977a. Vegetative anatomy of Maihuenia
(Cactaceae) with some theoretical discussions of ontogenetic changes in xylem cell types. Bulletin of the
Torrey Botanical Club 104: 35–48.
Gibson, A. C., and K. E. Horak. 1978. Systematic anatomy and phylogeny of Mexican columnar cacti. Annals
of the Missouri Botanical Garden 65: 999–1057.
Gibson, A. C., and P. S. Nobel. 1986. The Cactus Primer.
Harvard University Press, Cambridge, Massachusetts.
Goldstein, G., and P. S. Nobel. 1991. Changes in osmotic
pressure and mucilage during low-temperature acclimation of Opuntia ficus-indica. Plant Physiology 97:
954–961.
Goldstein, G., J. L. Andrade, and P. S. Nobel. 1991.
Differences in water relations parameters for the
chlorenchyma and the parenchyma of Opuntia ficusindica under wet versus dry conditions. Australian
Journal of Plant Physiology 18: 95–107.
Herrera-Cardenas, R., T. Terrazas, and S. Loza-Cornejo.
2000. Anatomía comparada del tallo y de la raíz de las
especies del género Neoevansia Marshall (Cactaceae).
Boletín de la Sociedad Botánica de México 67: 5–16.
Lloyd, F. E. 1919. The origin and nature of the mucilage in
the cacti and in certain other plants. American Journal
of Botany 6: 156–166.
Loza-Cornejo, S., and T. Terrazas. 1996. Anatomía del tallo
y de la raíz de dos especies de Wilcoxia Britton & Rose
(Cactaceae) del noroeste de México. Boletín de la
Sociedad Botánica de México 59: 13–23.
Loza-Cornejo, S., and T. Terrazas. 2001. Epidermal characteristics of North American Cactoideae. Journal of
Plant Research (in press).
Mauseth, J. D. 1978a. The structure and development of
an unusual type of articulated laticifer in Mammillaria
(Cactaceae). American Journal of Botany 65: 415–420.
Mauseth, J. D. 1978b. Further studies of the unusual type
of laticiferous canals in Mammillaria (Cactaceae):
Structure and development of the semi-milky type.
American Journal of Botany 65: 1098–1102.
Mauseth, J. D. 1980. Release of whole cells of Nopalea
(Cactaceae) into secretory canals. Botanical Gazette 141:
15–18.
Gibson, A. C. 1977b. Wood anatomy of opuntias with
cylindrical to globular stems. Botanical Gazette 138:
334–351.
Mauseth, J. D. 1984a. Introduction to cactus anatomy. Part
7. Epidermis. Cactus and Succulent Journal (U.S.) 56:
33–37.
Gibson, A. C. 1978. Wood anatomy of Platyopuntias. Aliso
9: 279–307.
Mauseth, J. D. 1984b. Introduction to cactus anatomy.
Part 8. Inner body. Cactus and Succulent Journal (U.S.)
56: 131–136.
Gibson, A. C. 1990. The systematics and evolution of subtribe Stenocereinae. 8. Organ pipe cactus and its clos-
38
est relatives. Cactus and Succulent Journal (U.S.) 62: 13–
24.
Terrazas Salgado and Mauseth
Mauseth, J. D. 1989. Comparative structure-function stud-
ies within a strongly dimorphic plant, Melocactus intortus (Cactaceae). Bradleya 7: 1–11.
Mauseth, J. D. 1992. Comparative wood anatomy of
Jasminocereus and Armatocereus in Ecuador and the
Galapagos Islands. IAWA Bulletin 13: 245–246.
Mauseth, J. D. 1993a. Medullary bundles and the evolution of cacti. American Journal of Botany 80: 928–932.
Mauseth, J. D. 1993b. Water-storing and cavitationpreventing adaptations in wood of cacti. Annals of
Botany 72: 81–89.
Mauseth, J. D. 1995a. Ontogenetic mechanisms and the
evolution of Cactaceae. Giornale Botanico Italiano 129:
429–435.
Mauseth, J. D. 1995b. Collapsible water-storage cells in
cacti. Bulletin of the Torrey Botanical Club 122: 145–151.
Mauseth, J. D. 1996. Comparative anatomy of tribes
Cereeae and Browningieae (Cactaceae). Bradleya 14:
66–81.
Mauseth, J. D. 1999a. Anatomical adaptations to xeric conditions in Maihuenia (Cactaceae), a relictual, leaf-bearing cactus. Journal of Plant Research 112: 307–315.
Mauseth, J. D. 1999b. Comparative anatomy of Espostoa,
Pseudoespostoa, Thrixanthocereus, and Vatricania (Cactaceae). Bradleya 17: 33–43.
Mauseth, J. D., and R. G. Ross. 1988. Systematic anatomy
of the primitive ceroid cactus Leptocereus quadricostatus. Bradleya 6: 49–64.
Mauseth, J. D., and M. Sajeva. 1992. Cortical bundles in
the persistent, photosynthetic stems of cacti. Annals of
Botany 70: 317–324.
Mauseth, J. D., and B. J. Plemons. 1995. Developmentally
variable, polymorphic woods in cacti. American Journal
of Botany 82: 1199–1205.
Mauseth, J.D., and R. Kiesling. 1997. Comparative anatomy of Neoraimondia roseiflora and Neocardenasia herzogiana (Cactaceae). Haseltonia 5: 37–50.
Mauseth, J. D., and J. V. Landrum. 1997. Relictual vegetative anatomical characters in Cactaceae: The genus
Pereskia. Journal of Plant Research 110: 55–64.
Mauseth, J. D., and B. J. Plemons-Rodriguez. 1998.
Evolution of extreme xeromorphic characters in wood:
A study of nine evolutionary lines in Cactaceae.
American Journal of Botany 85: 209–218.
Mauseth, J. D., Y. Uozumi, B. J. Plemons, and J. V.
Landrum. 1995. Structural and systematic study of an
unusual tracheid type in cacti. Journal of Plant Research
108: 517–526.
Mauseth, J. D., T. Terrazas, and L. Loza-Cornejo. 1998.
Anatomy of relictual members of subfamily Cactoideae
IOS group 1a (Cactaceae). Bradleya 16: 41–43.
Metcalfe, C. R., and L. Chalk. 1950. Anatomy of the Dicotyledons. Vol. II. Clarendon Press, Oxford.
Molina-Freaner, F., C. Tinoco-Ojanguren, and K. J.
Niklas. 1998. Stem biomechanics of three columnar
cacti from the Sonoran Desert. American Journal of
Botany 85: 1082–1090.
Niklas, K. J., and J. D. Mauseth. 1981. Relationships
among shoot apical meristem ontogenic features in
Trichocereus pachanoi and Melocactus matanzanus (Cactaceae). American Journal of Botany 68: 100–105.
Niklas, K. L., and S. L. Buchman. 1994. The allometry of
saguaro height. American Journal of Botany 81: 1161–
1168.
Niklas, K. L., F. Molina-Freaner, and C. TinocoOjanguren. 1999. Biomechanics of the columnar cactus Pachycereus pringlei. American Journal of Botany 86:
688–695.
Niklas, K. L., F. Molina-Freaner, C. Tinoco-Ojanguren,
and D. J. Paolillo, Jr. 2000. Wood biomechanics and
anatomy of Pachycereus pringlei. American Journal of
Botany 87: 469–481.
Nobel, P. S. 1988. Environmental Biology of Agaves and
Cacti. Cambridge University Press, New York.
Nobel, P. S. 1994. Remarkable Agaves and Cacti. Oxford
University Press, New York.
Nobel, P. S. 1999. Physicochemical and Environmental
Plant Physiology, 2nd ed. Academic Press, San Diego,
California.
Nobel, P. S., and R. W. Meyers. 1991. Biomechanics of
cladodes and cladode-cladode junctions for Opuntia
ficus-indica (Cactaceae). American Journal of Botany 78:
1252–1259.
Nobel, P. S., and E. De la Barrera. 2000. Carbon and water
balances for young fruits of platyopuntias. Physiologia
Plantarum 109: 160–166.
Nobel, P. S., J. Cavelier, and J. L. Andrade. 1992. Mucilage
in cacti: Its apoplastic capacitance, associated solutes,
and influence on tissue water relations. Journal of
Experimental Botany 43: 641–648.
Nyffeler, R., and U. Eggli. 1997. Comparative stem anatomy and systematics of Eriosyce sensu lato (Cactaceae).
Annals of Botany 80: 767–786.
Nyffeler, R., U. Eggli, and B. E. Leuenberger. 1997.
Noteworthy idioblastic sclereids in the stems of
Shoot Anatomy and Morphology
39
Eulychnia (Cactaceae). American Journal of Botany 84:
1192–1197.
Sattler, R. 1988. Homeosis in Plants. American Journal of
Botany 75: 1606–1617.
Parkhurst, D. F. 1986. Internal leaf structure: a three-dimensional perspective. In On the Economy of Plant
Form and Function (T. J. Givinish, ed.). Cambridge
University Press, New York. Pp. 215–249.
Terrazas, T. 2000. Perforated ray cells in genera of Pachycereeae (Cactoideae). IAWA Journal 21: 457–462.
Pimienta-Barrios, E., M. Loera-Quezada, and L. LópezAmezcua. 1993. Estudio anatómico comparativo en
colectas del subgénero Opuntia. Agrociencia serie
Fitociencia 4: 7–21.
Porembski, S. 1996. Functional morphology of Aztekium
ritteri (Cactaceae). Botanica Acta 109: 167–171.
Rivera, E. R. 1973. Echinocactus intertextus (Cactaceae): An
ultrastructural, physiological and biochemical study.
Ph.D. Thesis, University of Texas, Austin.
Sajeva, M., and J. D Mauseth. 1991. Leaf-like structure in
the photosynthetic, succulent stems of cacti. Annals of
Botany 68: 405–411.
40
Terrazas Salgado and Mauseth
Terrazas, T. 2001. Wood anatomy of Pachycereeae
(Cactoideae). IAWA Journal (submitted).
Terrazas, T., and S. Loza-Cornejo. 2002. Phylogenetic relationships of Pachycereeae: A cladistic analysis based
on anatomical-morphological data. In Evolution,
Ecology, and Conservation of the Columnar Cacti and
their Mutualists (T. H. Fleming and A. ValienteBanuet, eds.). University of Arizona Press, Tucson (in
press).
Wittler, G. H., and J. D. Mauseth. 1984. Schizogeny and
ultrastructure of developing latex ducts in Mammillaria guerreronis (Cactaceae). American Journal of
Botany 71: 1128–1138.
CHAPTER
›3‹
RO OT S T RU C T U R E
AND FUNCTION
Joseph G. Dubrovsky and Gretchen B. North
Introduction
Structure
Primary Structure
Secondary Structure
Root Types
Development and Growth
Indeterminate Root Growth
Determinate Root Growth
Lateral Root Development
Root System Development
Adaptations to Deserts and Other Arid Environments
Root Distribution in the Soil
Environmental Effects on Root Development
Developmental Adaptations
Water and Mineral Uptake
Root Hydraulic Conductivity
Mineral Uptake
Mycorrhizal and Bacterial Associations
Carbon Relations
Conclusions and Future Prospects
Literature Cited
Introduction
From the first moments of a plant’s life cycle, including
germination, roots are essential for water uptake, mineral
acquisition, and plant anchorage. These functions are especially significant for cacti, because both desert species
and epiphytes in the cactus family are faced with limited
and variable soil resources, strong winds, and frequently
rocky or sandy habitats. The goals of this chapter are to review the literature on the root biology of cacti and to present some recent findings. First, root structure, growth, and
development are considered, then structural and developmental adaptations to desiccating environments, such as
deserts and tropical tree canopies, are analyzed, and finally
the functions of roots as organs of water and mineral uptake are explored.
41
Structure
Cactus roots are less overtly specialized in structure than
are cactus shoots. Even so, root structural properties are
fundamental to the ability of cacti to take up water and nutrients quickly, and to endure and recover from drought.
An understanding of the relationship between root structure and function is essential to understanding how cacti
are able to occupy some of the driest, most nutrient-poor
habitats on earth.
Primary Structure
During embryogenesis, an embryonic root, or radicle, is
formed. In most cactus species, the radicle is relatively
small; for example, for Echinocactus platyacanthus the radicle is 320 µm long with a compact root cap of four cell
layers covering the tip (Lux et al. 1995). Similarly, a small
radicle is a typical feature in Astrophytum myriostigma,
Thelocactus bicolor (Engelman 1960), and Stenocereus gummosus (Dubrovsky 1997b). Meristematic activity at the
radicle apex begins approximately 12 hours after the radicle emerges from the seed coat for S. gummosus and Ferocactus peninsulae var. townsendianus (Dubrovsky 1997b). As
a result of activity in the root apical meristem, roots grow
in length, and the primary root tissues are formed (Esau
1977). The organization of the root apical meristem has
been analyzed fully for Opuntia basilaris (Freeman 1969)
and illustrated for a few other species. The roots of most
cacti appear to have a closed apical organization in which
each tissue can be traced to initial cells at the apex, as seen
for O. basilaris (Freeman 1969), O. arenaria (Boke 1979),
and E. platyacanthus (Lux et al. 1995).
Probably the best-studied species with respect to root
development and structure is O. ficus-indica. The radial pattern of the primary root structure in O. ficus-indica does not
differ significantly from that of most other dicotyledonous
species (North and Nobel 1996). For this species, the external tissue—the epidermis—is composed of compact cells,
some of which produce root hairs (Fig. 3.1A). Underlying
the epidermis is the cortical tissue complex, which includes
the hypodermis (the outermost cortical layer), the cortex
proper, and the endodermis (the innermost cortical cell
layer). The tissue complex located inward from the endodermis is the vascular cylinder. It comprises a two- or threecell-layered pericycle and the vascular system, consisting of
the xylem, the phloem, and the vascular parenchyma. The
root vasculature is polyarch, usually with five to seven xylem
poles in cylindropuntias (Hamilton 1970) and with four to
eight xylem poles in platyopuntias (Freeman 1969). The
pith is composed of parenchyma cells, as seen in O. basilaris
42
Dubrovsky and North
(Freeman 1969). Occasionally, mucilage cells are found in
the primary root (Hamilton 1970).Fgiunre31.eahrer:
Differentiation of primary tissues starts soon after cell
division stops in the meristem. For O. basilaris, the protophloem is first evident at 340 µm from the root cap–root
body junction; the protoxylem is first evident at 500 µm
and is fully differentiated at 1,400 µm. Casparian strips in
the endodermis occur at 500 µm from the junction. The
metaxylem begins to develop at the base of the transition
zone (region between the root and the hypocotyl) 4 to 5
days after germination and later can be found 1.2 mm from
the root apex (Freeman 1969). Primary tissue development
is unusually rapid in that as early as 6 days after germination the pericycle cells start to produce the periderm
(Freeman 1969), which is the first secondary tissue to develop in platyopuntia roots.
Secondary Structure
For O. ficus-indica, Ferocactus acanthodes, and two epiphytic cacti, Epiphyllum phyllanthus and Rhipsalis baccifera,
periderm layers (radially flattened cells just outside the pericycle) are well developed at about 150 to 200 mm from the
root tip in young roots. Even young seedlings of cylindropuntias have roots with several corky (suberized) layers
(Hamilton 1970). Such layers are more numerous and
more heavily suberized closer to the tip of roots that have
experienced drought than is the case for roots of well-watered plants (North and Nobel 1992). Back from the root
tip, in regions approximately 2 to 4 months old, the cortex external to the periderm dies and is shed (Fig. 3.1B), a
process that is also hastened by soil drying. Later in development, the outermost layers of the periderm are also shed
as the vascular cylinder enlarges due to secondary growth.
For the epiphyte R. baccifera, radial fissures open in the
outer suberized layers of the periderm as roots swell upon
re-watering after drought, thereby enhancing water uptake
(North and Nobel 1994).
Within the vascular cylinder of most cactus roots, secondary growth produces wedge-shaped regions of vessels
and fibers, separated by rays of parenchyma (Fig. 3.1C). For
several species, including platyopuntias such as O. ficusindica, large mucilage cells develop in the parenchyma
rays, with a possible consequence for regulating water relations within the vascular cylinder (Preston 1901b; Gibson
1973; North and Nobel 1992; Loza-Cornejo and Terrazas
1996). Other characteristics associated with the parenchyma in the secondary xylem can be the occurrence of calcium oxalate crystals (Fig. 3.1D), the storage of starch, and
the development of succulence. With respect to the xylem
vessels themselves, secondary growth leads to a nearly
A
B
C
D
Figure 3.1. Median cross-sections of (A) a 1-month-old root of Opuntia ficus-indica, showing primary root tissues; (B) a 3-month-old root of
Epiphyllum phyllanthus, with cortex separating from the periderm; (C) a 3-month-old root of O. ficus-indica, showing secondary growth; and
(D) a 5-month-old root of Rhipsalis baccifera, with arrow indicating a calcium oxalate crystal. Cell types shown are epidermis (ep), hypodermis
(h), cortex (c), endodermis (en), pericycle (p), periderm (per), and xylem (x). Scale bars: A = 50 µm, B = 500 µm, C–D = 100 µm.
threefold increase in mean vessel diameter for O. ficusindica and F. acanthodes, and a seven- to tenfold increase in
vessel number during 12 months of growth (North and
Nobel 1992). For the epiphytes E. phyllanthus and R. baccifera, mean vessel diameter increases only slightly during
3 months of growth, but vessel number also increases about
tenfold (North and Nobel 1994). Such increases in vessel
diameter and number are accompanied by large increases
in the rate of water transport in the xylem (North and
Nobel 1992, 1994).
Root Types
Different types of roots can be classified according to their
developmental origin. For example, a root that develops
from the embryonic radicle is termed a primary root.
Later, when the primary root reaches a certain length, lateral roots are formed. Any root formed on another root is
considered a lateral root. When a root is formed on an
organ other than a root, it is termed an adventitious root.
Cladodes of O. ficus-indica readily produce adventitious
roots at or near areoles (Fabbri et al. 1996; Dubrovsky et al.
1998b), reflecting localized activity in the vascular cambium (Villalobos 1995). For Pereskia, adventitious roots can
be formed on leaf petioles (Carvalho et al. 1989). Adventitious roots form along the stems of many decumbent,
prostrate, and epiphytic cacti, most of which never develop elongated primary roots (Gibson and Nobel 1986).
Adventitious rooting of fallen stem segments allows desert
Root Structure and Function
43
species, such as O. bigelovii, to reproduce vegetatively, and
the larger water storage capacity of such rooted segments
assures greater drought tolerance than is the case for much
smaller seedlings. For epiphytic cacti, adventitious rooting
along stems can improve anchorage in the canopy, and enables dislodged stem segments to take root where they land
on host species (Andrade and Nobel 1997). The ability to
produce adventitious roots is also useful for clonal propagation of O. ficus-indica and other agronomic species (Le
Houérou 1996).
Cactus roots can also be classified according to their
function and position within a root system. A century ago,
Carleton Preston from Harvard University defined anchoring versus absorbing roots in different cactus species
and found some anatomical differences in these root types
related primarily to the thickness of the vascular cylinder
(Preston 1900, 1901b). William Cannon from the Desert
Botanical Laboratory also used these terms, stating that anchoring roots can be: (1) vertically oriented, deeply penetrating, taproots; or (2) horizontally oriented, supporting
roots (Cannon 1911). Cannon divided absorbing roots into
two categories: (1) rope-like roots and (2) filamentous, relatively thin roots (Cannon 1911). This functional descriptive classification is not absolute, because each root type
can have a few functions simultaneously (Preston 1900;
Cannon 1911).
Two other root types with morphological modifications are succulent roots and tuberous storage roots, each
of which can have some characteristics of the other.
Cannon (1911) reported fleshy roots in O. vivipara, and
first recognized water storage capabilities of the roots of
some cactus species. Thick succulent roots (that occasionally include the hypocotyl–root transition zone) can be
found in small cacti, such as species of Ariocarpus (Britton
and Rose 1963; Bravo-Hollis and Sanchez-Mejorada 1978),
Aztekium (Porembski 1996), Leuchtenbergia (Britton and
Rose 1963), and Lophophora (Nobel 1994). For the columnar cactus Pachycereus pringlei, the fleshy taproot can be 18
cm thick near its base ( J. G. Dubrovsky, unpublished observations). Succulence develops within the secondary
xylem in Maihuenia patogonica, Nyctocereus serpentinus,
Opuntia macrorhiza, O. marenae, Pereskia humboldtii,
Pterocactus tuberosus, and Tephrocactus russellii, and in cortical ground tissue in Neoevansia diguetii and Peniocereus
greggii (Gibson 1978). Generally, water storage capacity (capacitance) is relatively small in cactus roots compared to
shoots (Nobel 1996). For succulent roots, however, the capacitance is greater than for nonsucculent roots, and may
be comparable to that of the water-storage parenchyma in
stems. Water-storage tissue in succulent roots has the abil-
44
Dubrovsky and North
ity to withstand a high degree of dehydration without irreversible damage, and may also help prevent water loss
and decrease root shrinkage during drought.
In addition to storing water, cactus roots frequently accumulate starch. To accommodate starch reserves, the roots
of some species acquire a distinct morphology. A relatively large, subterranean storage root is characteristic of cacti
that are geophytes; such roots give rise to aboveground annual shoots that shrivel and die during drought and are regenerated the following year, when water is available
(Gibson 1978; Gibson and Nobel 1986). Typical geophytes
in North America are species of Neoevansia, O. chaffeyi,
Peniocereus, and Wilcoxia, and in South America, Pterocactus tuberosus (Gibson 1978). Tuberous roots of Wilcoxia
poselgeri and W. tamaulipensis are characterized by starchstoring parenchyma, primarily in the cortex, along with
mucilage cells in the pith, the cortex, and the vascular tissue complex (Loza-Cornejo and Terrazas 1996). Tuberous
roots can be sizable; e.g., those of Peniocereus greggii grow
up to 60 cm in diameter, 15 to 20 cm long, and have a
weight of 27 to 56 kg (Britton and Rose 1963). Non-geophytes can also develop one or a few tuberous or tuber-like
roots. Groups of tuber-like roots 1 to 2.5 cm in diameter
occur for O. arbuscula (Cannon 1911), O. marenae, and O.
reflexispina (Felger and Moser 1985). Single tuber-like taproots occur for other non-geophyte species, such as
Ancistrocactus megarhizus (Britton and Rose 1963), Escobaria henricksonii (Glass 1998), Thelocactus mandragora
(Bravo-Hollis and Sanchez-Mejorada 1978), and T. subterraneus (Higgins 1948).
Another specialized root type—aerial roots—are rarely
produced by desert cacti. However, aerial roots can occur
for S. gummosus in the Sonoran Desert (Dubrovsky 1999).
This species has decumbent stems that form adventitious
roots when branches touch the soil. Aerial roots can develop before such contact, on the lower part of the convex
stem or on other portions of the stem (Fig. 3.2). These
roots are short, succulent, and sometimes extensively
branched, with secondary growth, and can be 3 to 4 mm
or more in diameter (Dubrovsky 1999). A possible role for
such roots in dew uptake remains to be studied. Under
greenhouse conditions, O. arenaria is also capable of
forming numerous aerial roots (Boke 1979). In epiphytic
and climbing species, aerial root development is a common
phenomenon, as in plants from the genera Epiphyllum,
Hylocereus, and Selenicereus (Bravo-Hollis and SánchezMejorada 1978).Fgiure32.neahrere:
Root systems can be composed of several different root
types and in many combinations. Nevertheless, three basic
morphological patterns of root systems are recognized
and nutrients. Increases in root surface area are the result
of two processes: (1) root elongation, which involves cell
production by the root apical meristem; and (2) root
branching, or the production of lateral roots. Cells within
the root apical meristem can proliferate for an indefinite
period, exhibiting indeterminate growth, or they can lose
such ability after a limited period, exhibiting determinate
growth. The amount and pattern of root branching depends, in part, on whether main roots are characterized by
indeterminate or determinate growth.
Figure 3.2. Aerial roots on the shoot of Stenocereus gummosus in the
Sonoran Desert.
(Cannon 1911; Gibson and Nobel 1986). The first type is
composed of a taproot with few or no lateral roots, as seen
for geophytes and species with succulent roots, such as in
the genera Lobivia and Lophophora (Gibson and Nobel
1986). The second type of root system is composed of a
taproot and horizontal, subsurface lateral roots and/or adventitious roots, as occurs for most columnar cacti and
species of Ferocactus (Cannon 1911; Gibson and Nobel
1986). The third type lacks a taproot and consists of roots
of different lengths, with small species tending to have numerous branched roots directly beneath the shoot, and
larger species tending to have long subsurface roots extended some length from the shoot, as seen in several
species of Opuntia (Gibson and Nobel 1986).
Development and Growth
Root development and growth are important, both during
the early stages of a plant’s life cycle (particularly for
seedling establishment) and, later, as continued shoot
growth requires that roots invade new areas to obtain water
Indeterminate Root Growth
Indeterminate root growth is common in most flowering
plants, including cacti. For example, adventitious roots of
Opuntia ficus-indica are characterized by indeterminate
growth, insofar as cell production by the root apical
meristem continues for a relatively long period. The tips of
main roots of O. ficus-indica generally die after a few
months of growth; however, death occurs more quickly in
dry soil than in wet soil (Dubrovsky et al. 1998b; G. B.
North, unpublished observations). By analyzing the cell
lengths along the root, three main root zones can be determined for O. ficus-indica: (1) the meristem (where cells
are relatively small and are in the cell division cycle), (2) the
elongation zone (where cells start and nearly complete
rapid elongation), and (3) the differentiation zone (where
cells complete their elongation and start to acquire certain
tissue characteristics). The root apical meristem of a main
adventitious root of O. ficus-indica is relatively large—on
average 1.1 mm long —consisting of 82 cortical cells in a cell
file (Dubrovsky et al. 1998b), comparable to the root meristem of most crop plants. The growing part of the root (the
meristem and the elongation zone) in this species is 5 to 7
mm long. The primary root of a Sonoran Desert species,
Pachycereus pringlei, exhibits indeterminate growth only
TA B L E 3 . 1
Growth characteristics of species with determinate root growth (Stenocereus gummosus and Ferocactus
peninsulae) and indeterminate root growth (Pachycereus pringlei ) in the first week of the seedling stage
Quantity
Primary root growth rate (µm hour –1)
Length of mature epidermal cells (µm)
Cell number in a file of epidermal cells
in the meristem
Duration of the cell division cycle (hours)
S. gummosus
F. peninsulae
P. pringlei
93 ± 9
98 ± 2
111 ± 9
85 ± 3
178 ± 7
176 ± 5
12.0 ± 0.2
9.7 ± 1.1
20.9 ± 0.4
11.7 ± 0.9
20.8 ± 0.4
14.5 ± 0.6
The duration of the cell division cycle is estimated by the rate of cell production method (Ivanov and
Dubrovsky 1997). Data are means ± SE (n = 11) and are from Dubrovsky et al. (1998a).
Root Structure and Function
45
primary root length (mm)
30
F. peninsulae
P. pringlei
S. gummosus
25
20
15
10
5
0
0
2
4
6
8
time (days)
Figure 3.3. Primary root length for Ferocactus peninsulae, Pachycereus
pringlei, and Stenocereus gummosus for 7 days after the start of radicle
protrusion. Data are means ± SE (n = 12) and are from Dubrovsky et
al. (1998a).
during the first week after germination (Fig. 3.3) and then
stops growing. The roots have much shorter meristems, averaging 21 epidermal cells in a cell file (Table 3.1). Smaller
root meristems are more typical for young seedlings, because meristem size is correlated with root diameter, and
primary and lateral roots are typically thinner than adventitious roots.Taben31l.eahrereF:giure3n.eahrere:
The rate of root growth depends on various factors. A
primary external factor is temperature, which affects the
duration of the cell cycle (cycle time). At an optimal temperature, the average cycle time in the root apical meristem
for O. ficus-indica is 28 hours (Dubrovsky et al. 1998b) and
for P. pringlei is 15 hours (Dubrovsky et al. 1998a). A primary internal factor is the final size of elongated cells. The
cell length for O. ficus-indica averages 378 µm (Dubrovsky
et al. 1998b) and for P. pringlei, 176 µm (Dubrovsky et al.
1998a). The average cell cycle time within the root apical
meristem can vary within and among species, but, in general and assuming elongated cells are the same size, the
shorter the cycle, the greater the rate of root growth
(Dubrovsky et al. 1998a).
Determinate Root Growth
Determinate root growth is the result of a developmental
program in the root apical meristem, in which a finite
number of cell division cycles takes place. For cacti, determinate root growth was first described for lateral roots of
Opuntia arenaria and O. tunicata var. davisii (Boke 1979).
46
Dubrovsky and North
Two types of determinate roots occur for these species.
First, relatively long lateral roots (a few cm long) are developed as a part of the absorptive or feeder root system.
The apical meristem of these roots has a normal organization, but functions for only a limited time. Soon the root
tip dies, and a new meristem is formed behind the dead
root tip, giving rise to a sympodially branched system of
roots of various lengths. On relatively long roots, short determinate roots of a second type, called “root spurs,” develop. The root spur is a cluster of sympodially branched
roots less than 1 mm long. The root tip of such spur roots
is atypical, lacking a root cap. All cells of the small apical
meristem become differentiated, and the tip of the spur
root becomes completely covered by root hairs. Spur roots
may allow a rapid increase in root surface area, increasing
water uptake during infrequent periods of rainfall (Boke
1979).
A determinate pattern of root growth in primary roots
is highly unusual for flowering plants. Indeed, continuous
elongation of primary roots seems generally more advantageous, particularly for seedling establishment. Surprisingly, determinate root growth is exhibited by the primary
root of Sonoran Desert cacti belonging to two tribes,
Pachycereeae (P. pringlei, Stenocereus thurberi, and S. gummosus; Dubrovsky 1997a,b) and Cacteae (Ferocactus peninsulae; Dubrovsky 1997b). Determinate growth of primary
roots is also shown by S. pruinosus and S. standleyi growing in the Tehuacán Valley of Mexico (Dubrovsky 1999). A
similar pattern occurs for epiphytic cacti, including Epiphyllum phyllanthus, in which the radicle dies after elongating by only 200 to 400 µm (G. B. North, unpublished
observations).
A typical characteristic of cactus roots with determinate growth is the relatively short duration of primary root
growth and early meristem exhaustion. For example, F.
peninsulae and S. gummosus grow for only 2 days after the
start of radicle protrusion (Fig. 3.3). When seedlings are
grown on filter paper, the radicle does not exceed 10 mm,
similar to its length in soil (Dubrovsky 1997a,b). When
seedlings are grown on sterile medium supplemented with
2% sucrose, the pattern of growth is the same, even
though the final size of the roots is greater (Dubrovsky
1997b). During the period of steady-state growth (24–36
hours), meristematic activity is maintained and the size of
the root apical meristem remains unchanged. After this period, meristematic cells cease dividing, leave the meristem,
and undergo rapid elongation. Because no new cells are
produced, the meristem rapidly decreases in size and becomes exhausted. As epidermal cells elongate and differentiate producing hairs, the root hairs grow to the tip of the
A
B
C
D
Figure 3.4. Root tips of Stenocereus gummosus at (A) 24 hours, (B) 36 hours, and (C) and (D) 48 hours after the start of radicle protrusion.
Root hairs (arrows) approach the tip in (A) and (B) and later cover the tip completely in (C). In (D), the five most apical cells (arrow) have
not formed root hairs. Scale bars: A = 400 µm, B–C = 100 µm, D = 50 µm.
root and subsequently cover it completely (Fig. 3.4;
Dubrovsky 1997b).Fgiure34.neahrere:
The size of the root apical meristem for cacti with determinate root growth is relatively small, with 12 to 21 cells
in a cell file (Table 3.1). The cells in the root apical meristem divide relatively quickly, every 10 to 14 hours for
Stenocereus gummosus and 12 to 17 hours for Ferocactus
peninsulae (Dubrovsky et al. 1998a). A comparison of the
duration of the period of steady-state growth (Dubrovsky
1997a,b) and the duration of the cell division cycle in the
root apical meristem (Dubrovsky et al. 1998a) shows that,
on average, only two cell division cycles occur in the root
apical meristem in both species. Assuming that meristematic activity is maintained until the meristem is exhausted,
the maximum number of cycles is four in S. gummosus and
five in F. peninsulae. Thus, the determinate pattern of root
growth in these cactus species represents a normal developmental path, during which only a few cell division cycles take place.
Lateral Root Development
For angiosperms, lateral roots originate mainly from the
pericycle of a parent root. For O. basilaris, the pericycle
cells opposite the protoxylem give rise to lateral root primordia early in seedling development (Freeman 1969). For
F. peninsulae and S. gummosus, lateral root primordia can
be detected 4 to 5 days after germination (Dubrovsky
1997a,b). The time from primordium initiation to lateralroot emergence is relatively short for these species and
comparable to that of other angiosperms (Dubrovsky
1997b). During lateral root development, a vascular junction between the parent root and a lateral root is established, consisting of relatively short tracheary elements
with large areas of non-lignified primary cell walls (North
et al. 1992), phloem elements, and vascular parenchyma
cells. Early in development, lateral root primordia are internal to the periderm, cortex, and epidermis of the parent
root (North et al. 1992; North and Nobel 1992). For
Ferocactus acanthodes, the inner layers of the periderm (the
phelloderm layers) of the parent root are continuous with
the base of the lateral root, whereas the suberized layers of
the periderm (the phellem layers) of the parent root are
ruptured as the lateral root elongates (North et al. 1992).
The initiation of lateral root primordia is apparently
promoted by drought. For example, the number of second-order lateral root primordia for F. acanthodes is
significantly greater for plants subjected to drought than
for plants under wet conditions (North et al. 1993). In distal root segments of O. ficus-indica, four to five more lateral root primordia develop during soil drying than under
48
Dubrovsky and North
wet conditions (Dubrovsky et al. 1998b). Similarly, the initiation of lateral root primordia in the epiphytes E. phyllanthus and Rhipsalis baccifera is stimulated by soil drying
(North and Nobel 1994). Because the rate of root growth
decreases during drought (Dubrovsky et al. 1998b), the occurrence of primordia closer to the root tip than under wet
conditions may reflect reduction in parent root growth, induction of lateral roots, or both. The developmental signals
for lateral-root initiation may also include changes in hormone levels due to the death of the parent root tip.
Lateral root elongation after drought is induced by rain
or watering, and such lateral roots have been called “rain”
roots, although a more general term is “ephemeral roots,”
because such roots tend to be short-lived (Nobel 1988).
Ephemeral roots can emerge rapidly, for example, within
8 hours of watering for both Opuntia puberula (Kausch
1965) and F. acanthodes (Nobel and Sanderson 1984).
Within 24 hours of watering O. ficus-indica after 14 days of
drought, lateral roots are 2 to 4 mm long, and lateral root
growth rate during the second day after emergence is 9.7
mm day-1 (Dubrovsky et al. 1998b). Apparently these roots
emerge from primordia formed during drought, but further analysis is needed. Ephemeral roots are important for
a rapid increase in absorbing surface area without a substantial increase in the distance for water transport
(Cannon 1911; Jordan and Nobel 1984; Dubrovsky et al.
1998b). For example, for F. acanthodes the total root length
increases by 27% because of ephemeral root formation
( Jordan and Nobel 1984). During subsequent drought, fine
lateral roots can abscise (North et al. 1993), and have thus
been called “deciduous roots” (Cannon 1911).
Root System Development
The type and extent of root systems in cacti can vary in response to both external and internal factors. For example,
cladodes of Opuntia versicolor planted in adobe (claycontaining) soil produce some vertically oriented anchoring roots and some horizontally oriented absorbing roots,
whereas cladodes planted in sand produce roots at seemingly random angles (Cannon 1925). The formation of a
root system is not necessarily a continuous process in a
desert. For example, a seedling may develop roots, then
lose them during a subsequent drought, and develop another root system upon re-watering. The duration of
drought, the shoot biomass (and thus water storage capacity), and the ability to form sequential root systems all
affect seedling survival for Stenocereus thurberi (Dubrovsky
1996, 1998, 1999). Most seedlings having fresh weights of
25 to 75 mg lose their root systems completely during a 40day drought. After rehydration, adventitious roots grow
from the basal portion of the hypocotyl, forming a new
root system that is larger than the original one (Dubrovsky
1999). Seedlings and young plants in the desert may develop a number of root systems before forming a lasting,
adult root system.
The architecture of a root system is partially determined by whether roots exhibit determinate or indeterminate growth. For plants with determinate primary root
growth (e.g., S. gummosus), some lateral roots appear to
have indeterminate growth. In an adult plant of this
species, long rope-like roots can be found, extending 5 to
6 m away from the plant (Dubrovsky 1999). Upon closer
inspection, however, such roots are not formed by continuous growth of the root apical meristem, but instead represent a series of interconnected lateral roots ( J. G.
Dubrovsky, unpublished observations), similar to sympodially branched roots described by Boke (1979).
Adaptations to Deserts and
Other Arid Environments
Root Distribution in the Soil
Most desert cacti can be classified as shallow-rooted perennials (Rundel and Nobel 1991). In the Sonoran Desert, the
roots of most cacti usually grow no deeper than 15 to 30 cm
below the soil surface, although the roots of some species
can extend laterally more than 10 m away from the plant
base (Cannon 1911). Not surprisingly, the deepest roots are
found for columnar cacti. A young plant of Carnegiea gigantea, 1.2 m tall, had a stout taproot that penetrated 30 cm
and lateral roots that extended 1.5 to 5 m away from the
plant, whereas an older, 6.8-m-tall plant had lateral roots
up to 9.7 m long that penetrated to a depth of 77 cm
(Cannon 1911), perhaps the deepest cactus roots on record.
Root proliferation and elongation is essential for continued water and mineral uptake, and for competition for
these resources with other plants. For Ferocactus acanthodes
at a site in the Sonoran Desert, the dry weight of the whole
root system averages only 14% that of the shoot; however,
the total surface area of the root system is about 3 times
greater than that of the shoot, and the total length of the
main roots in the root system per plant averages 182 m
( Jordan and Nobel 1984). Roots of Opuntia polyacantha
growing in the shortgrass steppe of Colorado have a median root depth of less than 2.5 cm (Dougherty et al. 1996).
For O. polyacantha, as little as 2.5 to 5 mm of precipitation
significantly increases cladode biomass. Indeed, the frequency of rain is more important than the absolute
amount of rain, due in part to competition with more
deeply rooted grasses that capture water from deeper soil
levels (Dougherty et al. 1996). In this case, a shallow root
distribution not only helps to exploit light rainfall, but also
gives the cactus an edge in competition with other plants.
Environmental Effects on Root Development
The root elongation rate depends on temperature. For F.
acanthodes, root growth in response to temperature can be
described by a bell-shaped curve, with maximal elongation
at 30oC ( Jordan and Nobel 1984). Cactus roots in natural
environments are frequently exposed to temperatures
higher than optimal; for example, in the northeastern
Sonoran Desert, the maximum temperature 5 to 10 cm
below the soil surface can be 40 to 50oC ( Jordan and
Nobel 1984). Cannon (1916) reported that at 43oC, roots
cease growing. At 60oC, the root cells of F. acanthodes die,
although high temperature tolerance of roots in this
species can be increased by acclimation. Roots of plants acclimated at day/night temperatures of 45/35oC survive at
temperatures 4oC higher than those of plants acclimated at
30/20oC ( Jordan and Nobel 1984).
Boulders and subterranean rocks, which are common
in desert environments, can provide favorable microsites
for cactus root systems. Cannon (1911) observed that
rocks stimulated root branching of Opuntia phaeacantha
var. discata in the Sonoran Desert. Similarly, roots of
Echinocereus engelmannii are more commonly found
alongside boulders than at increasing distances away, and
lateral roots of F. acanthodes are 5.5 times longer and 3
times more numerous under rocks than in regions of the
soil without rocks (Nobel et al. 1992b). Such increased
growth and branching are explained primarily by a longer
period of water availability, as the soil water potential decreases sharply with distance away from rocks and, after
soil wetting, remains higher under rocks than in rock-free
soil (Nobel et al. 1992b). In addition to creating locally
moist microsites, rocks may also be associated with regions
of higher nutrient concentrations. During active growth,
roots exude carbohydrates to the soil (Huang et al. 1993)
and, during subsequent drought, ephemeral roots die.
Organic matter may thus accumulate in the vicinity of
rocks, promoting new root growth near the rocks when
water is again available.
Soil drying generally decreases the rate of root growth,
although the rate of drying is critical in determining
whether apical elongation can continue. For example,
when Opuntia ficus-indica is subjected to gradual drying of
the substrate, roots have sufficient time for developmental
changes to occur, whereas rapid substrate drying leads to
death of the apical meristem. The meristem and elongation
zone become shorter when the substrate dries gradually,
Root Structure and Function
49
Figure 3.5. Root system of Epiphyllum phyllanthus after 21 days in
drying soil, in which all roots are covered by rhizosheaths.
whereas under rapid drying roots die because insufficient
time for such rearrangements is available (Dubrovsky et al.
1998b). Similarly, plants of F. acanthodes that have been
previously exposed to drought suffer less inhibition in root
growth under newly imposed water stress than do plants
that have not previously been so exposed ( Jordan and
Nobel 1984).
For a number of desert and epiphytic cacti, soil drying
also appears to enhance the development of rhizosheaths,
which are rough cylinders around the roots, composed of
soil particles that are bound to root hairs and other epidermal cells by mucilage exuded by the roots (Fig. 3.5;
Huang et al. 1993; North and Nobel 1992, 1994). Such rhizosheaths become thicker and more cohesive during
drought and help improve root water relations, both by ensuring good contact between the root and wet soil and by
helping to reduce water loss from the root to a drier soil
(North and Nobel 1997).Fgiure35n.eahrere:
Developmental Adaptations
Determinate root growth can be viewed as a developmental program well suited to desert conditions. For example,
meristem exhaustion in the primary root of Stenocereus
gummosus coincides with or perhaps triggers the initiation
of lateral root primordia. The percentage of roots bearing
lateral root primordia is correlated with the percentage
of roots in which the root apical meristem is exhausted
(Dubrovsky 1997a, 1997b). Determinate primary root
growth is thus a developmental mechanism leading to the
rapid induction of lateral root formation and root system
development. In most desert habitats, the optimum period for seed germination is extremely brief; thus, the rate of
root-system formation is a critical factor for successful
seedling establishment. Because many of the lateral roots
of S. gummosus also have determinate growth, a compact
50
Dubrovsky and North
root system is formed that requires limited carbon input
but is sufficient for water and mineral uptake (Dubrovsky
1997b, 1998).
A related developmental feature with adaptive significance is the relatively short duration of the cell cycle in the
root apical meristem for cacti with both determinate and
indeterminate root growth. Rapid root elongation and root
branching are possible only when new cells are produced
rapidly. A relatively short cell cycle can thus be advantageous, particularly during the critical stage of seedling establishment. At a later stage, when established roots resume both apical growth and branching after a drought, a
relatively short cell cycle with its high rate of cell production should also enhance the rate of colonization of new
soil regions by the roots.
Early root hair production, as seen for S. gummosus,
represents another developmental adaptation. For this
species, root hairs develop almost at the onset of seed germination. When the radicle is still very small, root hairs are
evident and are frequently longer than the radicle itself
(Fig. 3.4A). The basal epidermal cells, embryonic in origin,
average 18 µm in length, whereas the root hairs formed by
these cells average 100 times longer (1.8 mm). Each epidermal cell is capable of forming a root hair, unlike the
usual case for plants. Later, the epidermal cells that are
formed due to root meristem activity average 98 µm in
length (Table 3.1). Such early root hair formation, occurring even before germination is completed, can maximize
the root surface area available for absorption, thereby
increasing water and mineral uptake during the relatively short optimum growth period in a desert ( J. G.
Dubrovsky, unpublished observations).
Water and Mineral Uptake
Root Hydraulic Conductivity
For both desert and epiphytic cacti, soil moisture varies
greatly in both time and space. The success of cacti faced
with such heterogeneity in water availability depends on
the ability of their roots to conduct water quickly when it
is available, to resist water loss when the soil becomes dry,
and to resume water uptake upon the cessation of drought.
The ability of roots to absorb and transport water is
quantified by the root hydraulic conductivity, or LP. The
units of LP (m s-1 MPa-1, where MPa is 106 pascals) indicate
that a volume of water moves across the root surface area
per unit time in response to a difference in pressure (such
as the difference between the water potentials of the plant
and the soil; Nobel 1999). For roots, LP has two components, the radial conductivity, which determines the rate of
TA B L E 3 . 2
Hydraulic conductivity (LP) for young (1-month-old) and older (3- to 5-month-old)
roots of two terrestrial cacti (Ferocactus acanthodes and Opuntia ficus-indica) and two epiphytic cacti (Epiphyllum phyllanthus and Rhipsalis baccifera) under wet conditions, at 21
to 30 days in drying soil, and at 3 days after soil rewetting
LP (× 10–7m s–1 MPa–1)
Species
F. acanthodes
O. ficus-indica
E. phyllanthus
R. baccifera
Root age
Wet soil
young
older
young
older
young
older
young
older
2.4
4.2
1.7
5.4
2.3
5.6
2.1
7.0
Drying soil
Rewetted soil
1.8
1.6
1.4
1.3
0.7
2.6
0.7
3.2
2.0
2.6
1.9
1.4
2.8
5.4
2.5
8.0
Data are from North and Nobel (1992, 1994).
water flow across the root tissues up to the xylem (and
which generally is similar in value to LP), and axial conductivity, which determines the rate of flow through the
xylem.
Despite the ability of most cactus roots to endure
drought, their LP under wet soil conditions (Table 3.2) is
comparable to that of many mesophytic species, such as
Zea mays (1.0–2.8 × 10-7 m s-1 MPa-1; Steudle et al. 1987)
and Phaseolus vulgaris (1.4–4.0 × 10-7 m s-1 MPa-1; Sands et
al. 1982). Three- to 5-month-old roots of both terrestrial
and epiphytic cacti have a higher LP than do 1-month-old
roots under wet conditions, due to the higher resistance of
the cortex and the immaturity in the xylem of younger
roots (North and Nobel 1992, 1994, 1997). During 3 to 4
weeks of soil drying, LP decreases to about 50% of its value
under wet conditions, averaged for young and older roots
of the four cactus species (Table 3.2). The decrease is primarily due to reductions in radial conductivity, caused by
increased suberization in the periderm, and secondarily to
decreases in axial conductivity, due to embolism in the
xylem (North and Nobel 1992, 1994, 1996). During the
same period of soil drying, root diameter shrinks by 19%
for roots of Opuntia ficus-indica with rhizosheaths, by 26%
for bare roots of this species, and by 13% for older bare
roots of Epiphyllum phyllanthus (North and Nobel 1992,
1994). Such shrinkage causes air gaps to develop between
the root and the soil, which decrease water loss from the
root that would otherwise occur due to the higher water
potential of the root than of the soil during drought
(Nobel and Cui 1992).Tabe32l.neahrere:
In response to soil rewetting after drought, LP’s for
roots of both terrestrial and epiphytic cacti increase to
equal or exceed their pre-drought values, with the exception of older roots of Ferocactus acanthodes and O. ficusindica (Table 3.2). The layers of periderm in these older
roots increases in number and in extent of suberization
during drought, with a concomitant reduction in their permeability to water (North and Nobel 1992, 1996). Younger
root regions lack a periderm, and their permeability is restored by simple rehydration of tissues that, in addition,
are protected during drought by the rhizosheaths (North
and Nobel 1997). For the younger roots, and for root regions near the junctions between main and lateral roots,
numerous lateral root primordia arise during drought and
elongate upon rewetting. As these new lateral roots emerge
from the parent root, they break through the suberized layers of periderm, thereby increasing root permeability and
LP (North et al. 1993; Dubrovsky et al. 1998b). Once new
lateral roots have emerged, the root system for these cacti
is capable of rapid water uptake, allowing depleted storage
reservoirs to be refilled.
Mineral Uptake
The ability of roots to take up minerals is directly related
to their growth. In addition, root growth depends on the
nutrient status of the soil. When a mineral resource is in
limited supply in the soil, root systems increase in length
to explore more area around the plant. As a consequence,
relative root biomass (or the root/shoot ratio) tends to be
higher in poor soil than in richer soils (Marschner 1986).
Root Structure and Function
51
Such is the case for cactus plants. In the Sonoran Desert,
trees with relatively large canopies (e.g., Prosopis articulata) that serve as nurse plants for many cactus species
(Nobel 1988) tend to be “islands” rich in nutrients. The
concentration of nitrogen (N), phosphorous (P), and carbon (C) in such island soil is 1.4, 1.6, and 1.8 times greater,
respectively, than in a treeless region; correspondingly, the
root/shoot ratios for plants of Pachycereus pringlei grown in
island soil are smaller than for plants grown in soil from a
treeless region (Carrillo-Garcia et al. 2000).
Mineral uptake by roots can be assessed indirectly from
an analysis of the contents of different elements in cactus
stem tissues. By abundance, the elements in the stem tissue rank as follows: Ca > K > N > Mg > Na > P > Fe ≥ B
> Mn ≥ Zn > Cu > Mo, with the chlorenchyma having
higher levels of Ca, Mg, B, and Zn than are found in most
agronomic plants (Berry and Nobel 1985). Element concentrations in the cactus stem tissues can be more than 103
times greater than in the root substrate, implicating active
uptake and transport of ions by the roots (Berry and Nobel
1985; Kolberg and Lajtha 1997). Like cactus stems, roots
also accumulate certain elements in their tissues. For example, when boron (B) is applied at a concentration of 15
ppm to soil in which O. ficus-indica and F. acanthodes are
grown, the content of the element in the roots of both
species is about 145 ppm. Interestingly, the level of B increases to 2,000 ppm in stem tissues of O. ficus-indica and
to only 220 ppm in F. acanthodes (Berry and Nobel 1985),
indicating species differences in element translocation
from the root to the shoot. Different species can also vary
in their sensitivity to heavy metals. For example, when
high concentrations of copper (Cu) and zinc (Zn) are
added together to a substrate, the root dry weight of O.
ficus-indica is reduced more than that of F. acanthodes
(Berry and Nobel 1985).
Mycorrhizal and Bacterial Associations
Mineral acquisition is frequently related to the activity of
fungi and bacteria in the rhizospere. Mycorrhizal associations, important for mineral uptake in many plant species,
occur in the root systems of a number of cacti. The characteristic structures indicating infection by vesiculararbuscular mycorrhizae can be detected in a cleared, longitudinally dissected root of P. pringlei. During the first
stages of root colonization, the fungus forms an adherent
apressorium on the root surface. Subsequently, fungal hyphae penetrate the root, apparently multiplying the internal surface area available for the absorption of limiting
mineral nutrients such as P and iron (Fe). In the Sonoran
Desert, the level of mycorrhizal colonization ranges from
52
Dubrovsky and North
less than 10% of the roots examined in Mammillaria
dioica, P. pringlei, Stenocereus gummosus, and S. thurberi, to
30 to 70% in F. peninsulae, to more than 70% in Cochemia
poselgeri, Lophocereus schottii, Opuntia cholla, and O. lindsayi (Carrillo-Garcia et al. 1999). Extensive colonization by
three different fungal species in the genus Glomus also occurs for roots of Echinocactus acanthodes, Echinocereus engelmannii, O. acanthocarpa, O. basilaris, O. bigelovii, and
O. echinocarpa (Bethlenfalvay et al. 1984). Mycorrhizal associations are found in tropical forest cacti as well, including Nopalea karwinskiana, O. excelsa, and O. puberula
(Allen et al. 1998). For these forest species, mycorrhizal infection increases in proportion to fine root production,
which, in turn, is determined by the rainfall pattern
(Allen et al. 1998).
Free-living nitrogen-fixing bacteria from the genus
Azospirillum, present in the rhizosphere of many plant
species (Kapulnik 1996), have been isolated from cactus
roots as well. For example, A. lipoferum occurs in the rhizosphere of species of Opuntia growing in India (Rao and
Venkateswarlu 1982) and Mexico (Mascarua-Esprarza et al.
1988). Another species, A. brasilense, which occurs in the
rhizosphere of O. ficus-indica, S. pruinosus, and S. stellatus,
shows nitrogenase activity (ability to fix atmospheric nitrogen) and also exudes the plant hormone auxin, which
may induce root branching (Mascarua-Esprarza et al.
1988). When young seedlings of P. pringlei are inoculated
with A. brasilense, the bacteria survives in the plant rhizosphere for up to 300 days (Puente and Bashan 1993). In
another experiment, inoculation with A. brasilense increases root length but not shoot size, and nitrogenase activity is not detected (Carrillo-Garcia et al. 2000).
However, bacteria showing acetylene reduction activity
(indicative of nitrogenase activity) are eleven times more
abundant in the rhizosphere of ten species of cacti in
Mexico than in adjacent bare soil (Loera et al. 1996). A
likely role for rhizosheaths in providing conditions favorable to the growth of beneficial bacteria has yet to be explored for cacti.
Carbon Relations
In comparison with most other plants, cacti invest relatively little carbon into the construction and maintenance
of roots. This is partly due to the extremely small root/
shoot ratio of most succulents (Nobel 1988; Rundel and
Nobel 1991), particularly when expressed on a fresh weight
basis. It is also due to the relatively low rates of root respiration. Specifically, root respiration, as measured by total
CO2 efflux, is 0.7 and 0.3 mol CO2 kg-1 day-1 for young
and older roots, respectively, of Ferocactus acanthodes, and
1.1 and 0.5 mol CO2 kg-1 day-1 for young and older roots
of Opuntia ficus-indica (Palta and Nobel 1989). Comparable rates for the roots of twelve nondesert angiosperms
average 4.8 mol CO2 kg-1 day-1 (Lambers 1979). Under
drying conditions, root respiration for F. acanthodes and O.
ficus-indica declines even further, averaging 14% of the rate
under wet conditions 8 days after water is withheld (Palta
and Nobel 1989). The rate of growth respiration, measured
as CO2 given off by newly initiated roots, is also low for
cacti in comparison to other plants, averaging about 9 mol
CO2 kg-1 day-1 for F. acanthodes and O. ficus-indica (Nobel
et al. 1992a), in contrast to 24 mol CO2 kg-1 day-1 for nondesert angiosperms (Lambers 1979). Carbon costs are also
involved with maintaining mycorrhizal associations and
with the creation of rhizosheaths, although young sheathed
roots of O. ficus-indica exude only about 1% of newly fixed
carbon to the soil (Huang et al. 1993).
Conclusions and Future Prospects
Roots and root systems of cacti have evolved structural and
physiological features that permit them to withstand environmental stresses, such as high temperatures, prolonged
drought, nutrient-poor soils, and strong winds. Developmental adaptations, such as the early formation of root
hairs, lateral roots, and periderm, are most significant during the critical period of seedling establishment. The development of rhizosheaths is important for taking up water
from moist soil and reducing water loss to dry soil, and the
formation of lateral root primordia during drought hastens
plant recovery when soil moisture is restored. The shallow
distribution of roots in desert and grassland soils helps cacti
to exploit limited rainfall, at times in competition with
more deeply rooted neighboring plants. Root associations
with fungi and bacteria can help in the efficient capture of
limited mineral nutrients.
A number of structures and processes in roots of the
Cactaceae deserve further investigation. For example, a
century ago it was known that roots of Opuntia arbuscula
are capable of producing shoots (Preston 1901a), and similar “root buds” have been described for O. arenaria (Boke
1979). New shoots also appear to arise from the roots of
Myrtillocactus geometrizans ( J. G. Dubrovsky, unpublished
observations). Although root buds are a known phenomenon in angiosperms (Peterson 1975), their occurrence in
cacti has not been studied. Despite many accounts of
ephemeral roots, little is known about root phenology and
root plasticity in cacti. The relationship between root
growth and shoot activity and how it is affected by environmental variables, such as precipitation, needs to be investigated, particularly in the field. As an example, an un-
derstanding of how cactus roots respond to rain occurring
in the middle of a summer drought is important for predicting how desert communities will respond to possible
climate changes. Studies of mycorrhizal and bacterial associations with cactus roots will also help elucidate phenomena that are less well known for deserts and for tropical canopies than for other plant communities. The
nurse-plant association between cacti and other perennial
species deserves to be investigated from the perspectives of
root competition and root communication, both processes that may also be influenced by fungal and bacterial
activity.
In addition to the ecological questions remaining to be
addressed for cactus roots, certain basic developmental and
physiological processes should be explored for species that
can withstand prolonged water stress, such as Opuntia
ficus-indica. For example, the effects of soil drying on proteinaceous water channels (aquaporins) in the cell membranes of cactus roots can add to the current understanding of such channels in more mesophytic species. The
external and internal signals that trigger the initiation of
lateral root primordia and other developmental processes,
such as determinate root growth and early root hair formation, can be studied in cacti from a wide range of habitats. The role of cactus roots as intermediaries between relatively stable, succulent shoots and heterogeneous, often
desiccating soil suggests numerous stimulating possibilities
for future research.
Acknowledgment
J.G.B. thanks CONACyT, Mexico (grants 5277-N and
31832-N) for support.
Literature Cited
Allen, E. B., E. Rincón, M. F. Allen, A. Pérez-Jomenez,
and P. Huante. 1998. Disturbance and seasonal dynamics of mycorrhizae in a tropical deciduous forest in
Mexico. Biotropica 30: 261–274.
Andrade, J. L., and P. S. Nobel. 1997. Microhabitats and
water relations of epiphytic cacti and ferns in a lowland
neotropical forest. Biotropica 29: 261–270.
Bethlenfalvay, G. J., S. Dakessian, and R. S. Pacovsky.
1984. Mycorrhizae in a southern California desert:
Ecological implications. Canadian Journal of Botany
62: 519–524.
Berry, W. L., and P. S. Nobel. 1985. Influence of soil and
mineral stresses on cacti. Journal of Plant Nutrition 8:
679–696.
Boke, N. H. 1979. Root glochids and root spurs of
Root Structure and Function
53
Opuntia arenaria (Cactaceae). American Journal of
Botany 69: 1085–1092.
Bravo-Hollis, H., and H. Sánchez-Mejorada. 1978. Las
Cactáceas de México. Universidad Nacional Autónoma
de México, Mexico City.
Britton, N. L., and J. N. Rose. 1963. The Cactaceae. Dover,
New York.
Cannon, W. A. 1911. The Root Habits of Desert Plants.
Publication 131, Carnegie Institution of Washington,
Washington, D.C.
Cannon, W. A. 1916. Distribution of the cacti with especial
reference to the role played by the root response to soil
temperature and soil moisture. American Naturalist 50:
435–442.
Cannon, W. A. 1925. Physiological Features of Roots, with
Especial Reference to the Relation of Roots to Aeration of
the Soil. Publication 368, Carnegie Institution of
Washington, Washington, D.C.
Carrillo-Garcia, A., J.-L. León de la Luz, Y. Bashan, and
G. J. Bethlenfalvay. 1999. Nurse plants, mycorrhizae,
and plant establishment in a disturbed area of the
Sonoran Desert. Restoration Ecology 7: 321–335.
Carrillo-Garcia, A., Y. Bashan, E. Díaz-Rivera, and G. J.
Bethlenfalvay. 2000. Effects of resource-island soils,
competition, and inoculation with Azospirillum on survival and growth of Pachycereus pringlei, the giant cactus of the Sonoran Desert. Restoration Ecology 8: 65–73.
Carvalho, M.-A. M., W. R. Monteiro, and S. M. C.
Dietrich. 1989. Histological aspects of root formation
in petioles of detached leaves of Pereskia grandifolia
(Cactaceae): Natural conditions and effects of GA3 and
dark. Annals of Botany 63: 505–514.
Dubrovsky, J. G. 1996. Seed hydration memory in
Sonoran Desert cacti and its ecological implication.
American Journal of Botany 83: 624–632.
Dubrovsky, J. G. 1997a. Determinate primary root growth
in Stenocereus gummosus (Cactaceae), its organization
and role in lateral root development. In Biology of Root
Formation and Development (A. Altman and Y. Waisel,
eds.). Plenum, New York. Pp. 13–20.
Dubrovsky, J. G. 1997b. Determinate primary-root growth
in seedlings of Sonoran Desert Cactaceae; its organization, cellular basis, and ecological significance. Planta
203: 85–92.
Dubrovsky, J. G. 1998. Determinate root growth as an
adaptation to drought in Sonoran Desert Cactaceae. In
Radical Biology: Advances and Perspectives on the
54
Dubrovsky and North
Function of Plant Roots (H. E. Flores, J. P. Lynch, and
D. Eissenstat, eds.). American Society of Plant
Physiologists, Rockville, Maryland. Pp. 471–474.
Dubrovsky, J. G. 1999. Desarrollo de sistema radicular durante la ontogénesis de plantas del género Stenocereus
(Cactaceae). In El Pitayo en Jalisco y Especies Afines en
México (E. Pimienta-Barrios, ed.). Universidad de
Guadalajara, Fundación Produce Jalisco, Guadalajara,
Mexico. Pp. 133–146.
Dubrovsky, J. G., L. Contreras-Burciaga, and V. B. Ivanov.
1998a. Cell cycle duration in the root meristem of
Sonoran Desert Cactaceae as estimated by cell-flow
and rate-of-cell production methods. Annals of Botany
81: 619–624.
Dubrovsky, J. G., G. B. North, and P. S. Nobel. 1998b.
Root growth, developmental changes in the apex, and
hydraulic conductivity for Opuntia ficus-indica during
drought. New Phytologist 138: 75–82.
Dougherty, R. L., W. K. Lauenroth, and J. S. Singh. 1996.
Response of a grassland cactus to frequency and size of
rainfall events in a North American shortgrass steppe.
Journal of Ecology 84: 177–183.
Engleman, E. M. 1960. Ovule and seed development in
certain cacti. American Journal of Botany 47: 460–467.
Esau, K. 1977. Anatomy of Seed Plants. Wiley, New York.
Fabbri, A., A. Cicala, and A. Tamburino. 1996. Anatomy
of adventitious root formation in Opuntia ficus-indica
cladodes. Journal of Horticultural Science 71: 235–242.
Felger, R. S., and M. B. Moser. 1985. People of the Desert
and Sea. Ethnobotany of the Seri Indians. University of
Arizona Press, Tucson.
Freeman, T. P. 1969. The developmental anatomy of
Opuntia basilaris. I. Embryo, root, and transition
zone. American Journal of Botany 56: 1067–1074.
Gibson, A. C. 1973. Comparative anatomy of secondary
xylem in Cactoideae (Cactaceae). Biotropica 5: 29–65.
Gibson, A. C. 1978. Structure of Pterocactus tuberosus, a
cactus geophyte. Cactus and Succulent Journal (U.S.)
50: 41–43.
Gibson, A. C., and P. S. Nobel. 1986. The Cactus Primer.
Harvard University Press, Cambridge, Massachusetts.
Glass, C. E. 1998. Guide to the Identification of Threatened
Cacti of Mexico. Fidecomiso Fondo para la Biodiversidad, Mexico City.
Hamilton, M. W. 1970. The comparative morphology of
three Cylindropuntias. American Journal of Botany 57:
1255–1263.
Higgins, V. 1948. Root system of desert plants. Cactus and
Succulent Journal (U.S.) 20: 71–73.
doleacetic acid production by Azospirillum isolates
from Cactaceous plants. Plant and Soil 106: 91–95.
Huang, B., G. B. North, and P. S. Nobel. 1993. Soil
sheaths, photosynthate distribution to roots, and rhizosphere water relations for Opuntia ficus-indica.
International Journal of Plant Sciences 154: 425–431.
Nobel, P. S. 1988. Environmental Biology of Agaves and
Cacti. Cambridge University Press, New York.
Nobel, P. S. 1994. Remarkable Agaves and Cacti. Oxford
University Press, New York.
Ivanov, V. B., and J. G. Dubrovsky. 1997. Estimation of the
cell-cycle duration in the root meristem: A model of
linkage between cell-cycle duration, rate of cell production, and rate of root growth. International Journal
of Plant Sciences 158: 757–763.
Nobel, P. S. 1996. Ecophysiology of roots of desert plants,
with special emphases on agaves and cacti. In Plant
Roots: The Hidden Half, 2nd ed. (Y. Waisel, A. Eshel,
and U. Kafrafi, eds.). Marcel Dekker, New York. Pp.
823–844.
Jordan, P. W., and P. S. Nobel. 1984. Thermal and water relations of roots of desert succulents. Annals of Botany
54: 705–717.
Nobel, P. S. 1999. Physicochemical and Environmental
Plant Physiology, 2nd ed. Academic Press, San Diego,
California.
Kapulnik, Y. 1996. Plant growth promotion by rhizosphere
bacteria. In Plant Roots: The Hidden Half, 2nd ed. (Y.
Waisel, A. Eshel, and U. Kafkafi, eds.). Marcel Dekker,
New York. Pp. 769–781.
Nobel, P. S., D. M. Alm, and J. Cavelier. 1992a. Growth
respiration, maintenance respiration and structuralcarbon costs for roots of three desert succulents.
Functional Ecology 6: 79–85.
Kausch, W. 1965. Beziehungen zwischen Wurzelwachstum,
Transpiration und CO2-Gaswechsel bei einigen
Kakteen. Planta 66: 229–238.
Nobel, P. S., and M. Cui, 1992. Shrinkage of attached roots
of Opuntia ficus-indica in response to lowered water
potentials—predicted consequences for water uptake
or loss to soil. Annals of Botany 70: 485–491.
Kolberg, K., and K. Lajtha. 1997. Population dynamics and
trace metal biogeochemistry of the saguaro cactus
(Carnegiea gigantea) in the Sonoran Desert. Ecoscience
4: 223–231.
Lambers, H. 1979. Efficiency of root respiration in relation
to growth rate, morphology and soil composition.
Physiologia Plantarum 56: 18–22.
Le Houérou, H. N. 1996. The role of cacti (Opuntia spp.)
in erosion control, land reclamation, rehabilitation and
agricultural development in the Mediterranean Basin.
Journal of Arid Environments 33: 135–159.
Loera, T. M. L., J. M. Sánchez-Yañez, and J. J PeñaCabriales. 1996. Acetylene reduction activity on the
root of cactaceous plants. Revista Latinoamericana de
Microbiología 38: 7–15.
Loza-Cornejo, S., and T. Terrazas. 1996. Anatomía del tallo
y de la raíz de dos especies de Wilcoxia Britton & Rose
(Cactaceae) del Noroeste de México. Boletín de la
Sociedad Botánica de México 59: 13–23.
Lux, A., Mikus, M., Hanácková, Z., and J. Kristín. 1995.
Radicle of Echinocactus platyacanthus (Cactaceae).
Plant and Soil 167: 17–21.
Marschner, H. 1986. Mineral Nutrition of Higher Plants.
Academic Press, London.
Mascarua-Esparza, M. A., R. Villa-Gonzalez, and J.
Caballero-Mellado. 1988. Acetylene reduction and in-
Nobel, P. S., P. M. Miller, and E. A. Graham. 1992b.
Influence of rocks on soil temperature, soil water potential, and rooting patterns for desert succulents.
Oecologia 92: 90–96.
Nobel, P. S., and J. Sanderson. 1984. Rectifier-like activities
of roots of two desert succulents. Journal of Experimental Botany 35: 727–737.
North, G. B., F. W. Ewers, and P. S. Nobel. 1992. Main
root–lateral root junctions of two desert succulents:
Changes in axial and radial components of hydraulic
conductivity during drying. American Journal of Botany
79: 1039–1050.
North, G. B., and P. S. Nobel. 1992. Drought-induced
changes in hydraulic conductivity and structure in
roots of Ferocactus acanthodes and Opuntia ficus-indica.
New Phytologist 120: 9–19.
North, G. B., and P. S. Nobel. 1994. Changes in root hydraulic conductivity for two tropical epiphytic cacti as
soil moisture varies. American Journal of Botany 81: 46–
53.
North, G. B., and P. S. Nobel. 1996. Radial hydraulic conductivity of individual root tissues of Opuntia ficusindica (L.) Miller as soil moisture varies. Annals of
Botany 77: 133–142.
North, G. B., and P. S. Nobel. 1997. Drought-induced
Root Structure and Function
55
changes in soil contact and hydraulic conductivity for
roots of Opuntia ficus-indica with and without rhizosheaths. Plant and Soil 191: 249–258.
North, G. B., B. Huang, and P. S. Nobel. 1993. Changes in
structure and hydraulic conductivity for root junctions
of desert succulents as soil water status varies. Botanica
Acta 106: 126–135.
Palta, J. A., and P. S. Nobel. 1989. Influences of water status, temperature, and root age on daily patterns of root
respiration for two cactus species. Annals of Botany 63:
651–662.
Porembski, S. 1996. Functional morphology of Aztekium
ritteri (Cactaceae). Botanica Acta 109: 167–171.
Peterson, R. L. 1975. The initiation and development of
root buds. In The Development and Functions of Roots
( J. G.Torrey and D. T. Clarkson, eds.). Academic
Press, London. Pp. 125–161.
Preston, C. E. 1900. Observations on the root system of
certain Cactaceae. Botanical Gazette 30: 348–351.
Preston, C. E. 1901a. Non-sexual propagation of Opuntia.
Botanical Gazette 31: 128.
Preston, C. E. 1901b. Structural studies on southwestern
Cactaceae. Botanical Gazette 32: 35–55.
Puente, M.-E., and Y. Bashan. 1993. Effect of inoculation
with Azospirillum brasilense strains on the germination
56
Dubrovsky and North
and seedling growth of the giant columnar cardon cactus (Pachycereus pringlei). Symbiosis 15: 49–60.
Rao, A. V., and B. Venkateswarlu. 1982. Associative symbiosis of Azospirillum lipoferum with dicotyledonous
succulent plants of the Indian Desert. Canadian
Journal of Microbiology 28: 778–782.
Rundel, P. W., and P. S. Nobel. 1991. Structure and function in desert root systems. In Plant Root Growth. An
Ecological Perspective (D. Atkinson, ed.). Blackwell
Scientific, London. Pp. 349–378.
Sands, R., E. L. Fiscus, and C. P. P. Reid. 1982. Hydraulic
properties of pine and bean roots with varying degrees
of suberization, vascular differentiation and mycorrhizal infection. Australian Journal of Plant Physiology
9: 559–569.
Steudle, E., R. Oren, and E.-D. Schulze. 1987. Water transport in barley roots. Measurement of hydraulic conductivity, solute permeability, and of reflection
coefficients of excised roots using the root pressure
probe. Plant Physiology 84: 1220–1232.
Villalobos, A. V. M. 1995. Tissue culture application for
Opuntia sp. pl. micropropagation. In Agro-Ecology,
Cultivation and Uses of Cactus Pear (G. Barbera, P.
Inglese, and E. Pimienta-Barrios, eds.). FAO Plant
Production and Protection Paper 132. FAO, Rome. Pp.
71–77.
CHAPTER
›4‹
E N V I RO N M E N TA L B I O LO G Y
Park S. Nobel and Edward G. Bobich
Introduction
Early Research
Net CO2 Uptake
Water-Use Efficiency
Net CO2 Uptake: Stems
Temperature
Water
PPF
Nutrients and Salinity
Atmospheric CO2
Net CO2 Uptake: Fruits
Productivity Responses to Environmental Factors
Environmental Productivity Index
Application of EPI to a Barrel Cactus
Application of EPI to a Cultivated Platyopuntia
Survival
Temperature
Water
Salinity
Gravity and Wind
Conclusions and Future Prospects
Literature Cited
Introduction
Early Research
The Desert Botanical Laboratory of the Carnegie Institution of Washington located just outside Tucson, Arizona,
was a major location for early environmental research on
cacti, with approximately 100 publications on environmental responses of cacti from its founding in 1903 to its
closure in 1940 (McGinnies 1981). The topics studied in-
cluded root growth, drought tolerance, temperature tolerance, and photosynthesis. Early experiments showed cacti
to be limited in growth by low but not by high temperatures, to conserve water, to require much light, and to have
special metabolic activity at night.
For instance, root growth of Opuntia versicolor was
shown to be maximal at 33°C, decreasing 50% at 19°C
(Cannon 1925). Ferocactus wislizenii could sustain 18
months of drought, during which it lost 70% of its initial
57
Net CO2 Uptake
Although O. ficus-indica has received the most research attention, net CO2 uptake has been measured for species in
all three traditional subfamilies of the Cactaceae (Table 4.1)
and for all four subfamilies if Maihuenioideae is separated
out of the Pereskioideae. For the Pereskioideae broadly
defined, the leaves are the dominant photosynthetic organs, and net CO2 uptake occurs almost entirely during
the daytime using the C3 photosynthetic pathway.
Maximal rates of net CO2 uptake of 4 to 6 µmol m-2 s-1 for
Maihuenia poeppigii, Pereskia aculeata, and P. grandifolia
occur in the early morning, whereas the stems always have
a net CO2 loss (Nobel and Hartsock 1987). Similar to the
Pereskioideae, many of the more primitive species of
Opuntioideae have prominent leaves. Maximum net CO2
uptake rates for leaves of Austrocylindropuntia subulata,
Pereskiopsis porteri, and Quiabentia chacoensis are about 3
µmol m-2 s-1 during the daytime (Table 4.1). However, in
58
Nobel and Bobich
20
net co 2 uptake (µmol m -2s -1 )
water content, and could survive indoors for 6 years without water (MacDougal et al. 1915). Its osmotic pressure was
about 0.4 MPa under wet conditions, increasing to 1.0
MPa after 6 months of drought (MacDougal and Cannon
1910). Maximum tissue acidity was shown to occur in
Carnegiea gigantea and Ferocactus acanthodes in the morning (Long 1915). Indeed, tissue acidity steadily increases
about threefold during the night and reversibly decreases
during the daytime for Mammillaria grahamii and O. versicolor (Richards 1915). When the stomates in stems of C. gigantea open at night, the stems contract (MacDougal 1924;
MacDougal and Working 1933). Stems of Opuntia phaeacantha var. discata tolerate 1 hour at 62°C (MacDougal and
Working 1922). With respect to low temperature, C. gigantea tolerates –8°C but not –10°C (Shreve 1931). Cacti
also “require all the light that it is possible to give them, the
more direct sunshine the better” (Shreve 1931).
In addition to the insights gained from research at the
Desert Botanical Garden, other early studies had relevance
to the environmental responses of cacti. For instance,
cladodes of Opuntia leucotricha tend to orient perpendicularly to the direction of a light beam (Goebel 1895).
Opuntia ficus-indica is injured at –5°C by ice crystals that
are initiated extracellularly (Uphof 1916). Moreover, O.
ficus-indica has a productivity of up to 20 tons dry mass
hectare-1 year-1 (Griffiths 1915). However, the main research
on the gas exchange and other environmental responses of
cacti did not occur until after World War II, when instruments were developed to measure net CO2 uptake, transpiration, radiation, temperature, and water potential,
using newly invented sensors and electronic circuitry.
Opuntia ficus-indica
15
O. humifusa
10
O. polycantha
5
0
-5
0
4
8
12
16
20
24
time of day (hour)
Figure 4.1. Net CO2 uptake over 24-hour periods for various platyopuntias. Hatched bars indicate nighttime. Data for Opuntia ficusindica are adapted from Cui et al. (1993) and Cui and Nobel (1994),
those for O. humifusa from Koch and Kennedy (1980), and those for
O. polyacantha from Gerwick and Williams (1978).
contrast to the case for the stems of species of Pereskioideae, stems of all three leafy species of Opuntioideae had
periods of positive net CO2 uptake, although maximal values averaged only 1 µmol m-2 s-1 (Nobel 1988).Tabe4ln1.eahrere:
For opuntias, the largest genus in the Opuntioideae,
leaves tend to be ephemeral and hence do not contribute
substantially to net CO2 uptake by the entire shoot. Net
CO2 uptake thus occurs primarily by the stems at night
(termed Phase I; Osmond 1978) in the Crassulacean acid
metabolism (CAM) mode (Fig. 4.1). Some aspects of the
gas exchange by an Opuntia sp. in Phase I were appreciated by Nicolas Théodore de Saussure in 1804, perhaps the
earliest experimental insight into CAM (Richards 1915).
The CO2 taken up is incorporated into an organic acid
such as malate using the enzyme phosphoenol pyruvate
carboxylase (PEPCase), and the accumulating acids are sequestered into the large vacuoles of chlorenchyma cells
(Kluge and Ting 1984). Thus the chlorenchyma becomes
progressively more acidic during the night for CAM
plants, and its osmotic pressure also increases (Lüttge and
Nobel 1984). Under wet conditions and moderate temperatures, some net CO2 uptake tends to occur in the early
morning using 1,5-ribulosebisphosphate carboxylase/oxygenase (Rubisco) via the C3 pathway before stem and air
temperatures have risen appreciably from their minimum
nighttime values (Phase II). In fact, carbon isotope evidence indicates that Opuntia polyacantha utilizes this phase
of the CAM pathway more during wetter, cooler periods
around 40,000 years before present (BP) than at 10,000
years BP or at the present time (Troughton et al. 1974).
TA B L E 4 . 1
Summary of maximum rates and total daily net CO2 uptake for cacti
Subfamily and species
Pereskioideae
Maihuenia poeppigii (leaf )
Pereskia aculeata (leaf )
Pereskia grandifolia (leaf )
Opuntioideae
Austrocylindropuntia subulata (leaf )
Opuntia acanthocarpa
O. basilaris
O. erinacea
O. ficus-indica
O. humifusa
O. phaeacantha
O. polyacantha
O. stricta
Pereskiopsis porteri (leaf )
Quiabentia chacoensis (leaf )
Cactoideae
Carnegiea gigantea
Cereus validus
Cryptocereus anthonyanus
Echinocereus engelmannii
Epiphyllum oxypetalum
E. pittieri
Ferocactus acanthodes
Hylocereus undatus
Mammillaria dioica
Pachycereus pringlei
Rhipsalis houlletiana
R. rhombea
Schlumbergera truncata
Stenocereus queretaroensis
Maximum net
CO2 uptake rate
(µmol m –2 s –1)
Total daily net
CO2 uptake
(mmol m –2 day –1)
4.3
4.7
6.0
159
130
202
Nobel and Hartsock (1987)
"
"
3.2
5.5
6.2
5.3
18.0
8.4
3.2
2.9
13.5
2.4
3.1
91
187
241
146
698
283
—
107
506
96
103
Nobel and Hartsock (1986a)
Nobel et al. (1991)
Hanscom and Ting (1978)
Littlejohn (1983)
Cui et al. (1993); Cui and Nobel (1994)
Koch and Kennedy (1980)
Nisbet and Patten (1974)
Gerwick and Williams (1978)
Osmond et al. (1979)
Nobel and Hartsock (1986a)
"
—
5.8
3.0
2.9
2.2
2.0
6.0
5.1
5.5
6.5
3.0
2.1
3.2
11.2
192
181
60
98
46
61
164
214
91
297
104
59
65
317
Nobel and Hartsock (1986a)
Nobel et al. (1984)
Nobel and Hartsock (1990)
Nobel et al. (1991)
Nobel and Hartsock (1990)
"
Nobel and Hartsock (1986a)
Raveh et al. (1995)
Nobel (1978); Nobel and Hartsock (1986a)
Franco-Vizcaíno et al. (1990)
Nobel and Hartsock (1990)
"
"
Nobel and Pimienta-Barrios (1995);
Pimienta-Barrios and Nobel (1998)
Reference
Data are restricted to those expressed on a unit surface area basis and under essentially optimal environmental conditions. For a more
complete coverage of the earlier literature, see Nobel (1988). Values are for stems unless otherwise indicated.
Usually there is no net CO2 uptake during the middle of
the daytime, but rather a slight loss occurs (Phase III; Fig.
4.1); at this time the acids accumulated the previous night
are decarboxylated, and the CO2 released within the
plant is refixed in the stems using Rubisco. The high internal concentration from this released CO2 causes the
daytime leakage of CO2 from the stems. Stomates tend to
reopen in late afternoon after stem temperatures have decreased, leading to net uptake and fixation of CO2 using
Rubisco (Phase IV; Fig. 4.1).Fgiure4n1.eahrere:
Maximal instantaneous rates of net CO2 uptake are
about 3 µmol m-2 s-1 for Opuntia polyacantha, which is native to the central United States, 8 µmol m-2 s-1 for O. humifusa, the mostly widely distributed native opuntia in the
United States, and 18 µmol m-2 s-1 for O. ficus-indica, the
most widely cultivated cactus worldwide (Fig. 4.1). Species
in the largest subfamily, Cactoideae, also have net CO2 uptake by stems at night in the CAM mode. Maximal nighttime net CO2 uptake rates are high for Cereus validus,
Ferocactus acanthodes, Mammillaria dioica, and Pachycereus
Environmental Biology
59
60
pringlei (average about 6 µmol m-2 s-1) and Stenocereus
queretaroensis (11 µmol m-2 s-1; Table 4.1). Maximal net
CO2 uptake rates for epiphytic cacti, such as species of
Cryptocereus, Epiphyllum, Rhipsalis, and Schlumbergera,
are much lower (2–3 µmol m-2 s-1), although the cultivated, shade-tolerant, vinelike, hemiepiphytic cactus Hylocereus undatus can have maximal rates of 5 µmol m-2 s-1
(Table 4.1). Values for total daily net CO2 uptake under
optimal environmental conditions are consistent with
maximal instantaneous rates of net CO2 uptake for cacti.
Among the cacti investigated, maximal total daily net CO2
uptake occurs for opuntias (698 mmol m-2 day-1 for O.
ficus-indica and 506 mmol m-2 day-1 for Opuntia stricta)
and S. queretaroensis (317 mmol m-2 day-1; Table 4.1).
midity at 20°C, the leaf-to-air difference in water vapor
concentration (the force leading to water loss by transpiration) is 12.8 – 8.0 or 4.8 g m-3 at 15°C and 23.1 – 8.0 or
15.1 g m-3 at 25°C, which is threefold greater at the higher
temperature. Therefore, transpiration would be threefold
greater at a daytime temperature of 25°C than for the same
degree of stomatal opening at night at 15°C. This is a key
feature for the water-conserving nature of CAM used by
cacti in arid and semiarid regions. In particular, because tissue temperatures in the field tend to average about 10°C
lower at night than during the daytime, CAM plants tend
to lose only 20 to 35% as much water for the same degree
of stomatal opening during the principal periods of net
CO2 uptake as do C3 or C4 plants.
Water-Use Efficiency
Net CO2 Uptake: Stems
The importance of nocturnal stomatal opening and the accompanying net CO2 uptake with regard to ecological or
agronomic success of cacti relates less to improving CO2
acquisition than to reducing water loss. In particular, air
and stem temperatures are lower at night, which leads to
a lower concentration of water vapor in the stem and hence
less water loss for a given degree of stomatal opening; this
is important in arid regions (less than 250 mm annual rainfall) or semiarid regions (250 to 450 mm annual rainfall).
The ratio of CO2 fixed to water loss in transpiration is
termed the water-use efficiency, which is an important
benefit:cost index for assessing the gas-exchange performance of plants. For stems of cacti under favorable environmental conditions with predominantly nocturnal net CO2
uptake, approximately 10 mmol of CO2 is taken up per
mol of water transpired over a 24-hour period. This wateruse efficiency is about three times higher than for highly
efficient C4 species such as Zea mays (maize) and Saccharum officinarum (sugarcane) and five times higher than
for highly efficient C3 species such as Medicago sativa (alfalfa) and Triticum aestivum (wheat; Nobel 1991).
To better understand the reason water loss is reduced
at night due to lower temperatures, consider the watervapor concentration at saturation (as essentially occurs
within the stems of cacti). The saturation water vapor content of air increases nearly exponentially with increasing
temperature, whereas the water content of the ambient air
surrounding a plant remains relatively constant unless the
weather abruptly changes. In particular, air saturated with
water vapor at 15°C, which can represent nighttime temperatures, contains 12.8 g m-3; at 25°C, which can represent
daytime temperatures, air saturated with water vapor contains 23.1 g m-3 (Nobel 1999). If the ambient air contains
8.0 g water m-3, which corresponds to 46% relative hu-
Net CO2 uptake, which has been determined per unit surface area for about 20 species of cacti (Table 4.1), depends
on several environmental factors. The three key environmental factors for drought-enduring plants are temperature, soil moisture, and the solar irradiation absorbed by
photosynthetic pigments, i.e., the photosynthetic photon
flux (PPF), which represents wavelengths of 400 to 700
nm. The response of net CO2 uptake by Opuntia ficusindica, the most widely studied cactus, to these three variables (Fig. 4.2) is important for predicting its productivity under any environmental condition and serves as a
model for assessing the net CO2 uptake, and hence the potential biomass productivity, of other cacti.Fgiure42.neahrere:
Nobel and Bobich
Temperature
The daily pattern and the magnitude of total net CO2 uptake by O. ficus-indica (Fig. 4.1) mainly reflect nocturnal
temperatures. In particular, PEPCase is more important
for the initial binding of CO2 than is Rubisco. The optimal mean nocturnal temperature is relatively low, 15°C
(Fig. 4.2A). Moreover, substantial net CO2 uptake occurs
at 0°C for O. ficus-indica, and O. humifusa can even have
substantial net CO2 uptake at air temperatures of –5°C
(Nobel and Loik 1990). Thus, low nighttime temperatures
are not disadvantageous for net CO2 uptake by these cacti,
whereas high nighttime temperatures, such as those above
30°C (Fig. 4.2A), can lead to appreciable stomatal closure
and hence limited net CO2 uptake.
Although most cacti examined have a low temperature
optimum (near 15°C) for net CO2 uptake, epiphytic cacti
native to the tropical areas of the Americas are subject to
much higher mean nocturnal temperatures for most of the
year and can acclimate to higher stem temperatures for
their PEPCase activity. For instance, Hylocereus undatus has
ature (usually 3ºC to 6ºC lower than the mean temperature) is more readily available from weather records and
can be correlated with net CO2 uptake as well.
A
1.0
daily net co 2 uptake per unit stem area (fraction of maxima)
0.8
0.6
Water
0.4
0.2
0.0
0
10
20
30
mean nighttime air temperature (°c)
B
1.0
0.8
0.6
0.4
0.2
0.0
0
10
20
30
40
50
drought length (days)
C
1.0
0.8
0.6
As the water content of the soil declines and its water potential decreases below that of the plant, thermodynamic
theory predicts that water will move from the plant to the
soil (Nobel 1999). Water is also continually being lost from
the shoot of a plant to the atmosphere. The inevitable loss
of stem water as the soil dries inhibits cellular processes
that lead to net CO2 uptake (Fig. 4.2B). In particular, little change in net CO2 uptake occurs during the first 7 days
of drought for O. ficus-indica because of the water stored
in its cladodes and the high water-use efficiency of the
CAM pathway. Daily net CO2 uptake then halves over the
next 17 days of drought and becomes near zero at 50 days
(Fig. 4.2B); the gradual decline in part reflects the inherently low transpiration rates resulting from a low stomatal
frequency and a thick cuticle. The response to soil water
content can be used to devise irrigation schedules for O.
ficus-indica and other cultivated cacti in arid and semiarid
regions and to discern seasonal patterns of net CO2 uptake
for native cacti in deserts.
0.4
PPF
0.2
0.0
0
10
20
30
40
total daily ppf (mol m -2 day -1 )
Figure 4.2. Influence of three environmental factors on net CO2
uptake by O. ficus-indica over 24-hour periods: (A) temperature, in
particular the mean nighttime air temperature, which was generally
within 1°C of the temperature in the middle of the chlorenchyma;
(B) water availability, measured by drought length, defined as when
the stem water potential becomes less than the soil water potential
adjacent to the roots, preventing plant water uptake; and (C) solar
irradiation that can be absorbed by photosynthetic pigments, known
as PPF (wavelengths of 400 to 700 nm, i.e. blue to red). Except
when that specific factor was varied, the plants were under essentially
optimal conditions of nighttime temperatures near 15°C, wet soil,
and a total daily PPF of about 25 mol m-2 day-1 incident on the
cladode surfaces. Data are from Nobel and Hartsock (1983, 1984,
1986b), Nobel and Israel (1994), and Israel and Nobel (1995).
maximum nocturnal net CO2 uptake at 25°C (Raveh et al.
1995). Just as high temperatures have relatively little
influence on the survival and distribution of cacti, high
daytime air and stem temperatures are not critical for daily
net CO2 uptake by cacti (Nobel 1988). Although the mean
nighttime temperature is more useful for quantifying nocturnal net CO2 uptake, the minimum nighttime temper-
Net CO2 uptake is negative for stems maintained continually in the dark (Fig. 4.2C), as only respiration occurs
under such circumstances because photosynthesis requires
light. Net CO2 uptake increases as the total daily PPF increases and becomes half-maximal at about 13 mol m-2
day-1 for O. ficus-indica (Fig. 4.2C; because most CO2 uptake occurs at night, data on the light responses of cacti are
generally based on the total daily PPF). Net CO2 uptake
approaches light saturation at about 30 mol m-2 day-1,
which is approximately half the total daily PPF incident on
a horizontal surface on a clear day with the sun passing
overhead at noon (the instantaneous PPF is then about
2,000 µmol m-2 s-1) but about equal to that incident on a
vertical surface of a cactus stem facing east or west on such
a day (Nobel 1988). For the shade-tolerant hemiepiphyte
Hylocereus undatus, on the other hand, net CO2 is halfmaximal at only 4 mol m-2 day-1, maximal at 20 mol m-2
day-1, and is 40% of maximal at 30 mol m-2 day-1 (Raveh
et al. 1995). For the holoepiphyte Schlumbergera truncata,
photosynthesis is inhibited at even lower light levels, with
daily net CO2 uptake being 19% lower for plants exposed
to a total daily PPF of 17 mol m-2 day-1 than for those exposed to 9 mol m-2 day-1 (Nobel and Hartsock 1990).
Indeed, epiphytic cacti generally do better under shaded
Environmental Biology
61
conditions, both in the field and under cultivation (Mizrahi
et al. 1997).
One reason that unshaded nonepiphytic cacti generally do not exhibit chronic photoinhibition (damage to the
light-dependent processes of photosynthesis due to excess
PPF; Taiz and Zeiger 1998) is that most of their photosynthetic surfaces are vertical and thus not perpendicular to
the incoming PPF. Spines and pubescence can also create
considerable shading for the stems of many cacti; in particular, the apices of certain ecotypes of Ferocactus acanthodes are exposed to only 10% of the incoming PPF
(Nobel 1980). The recycling of internal CO2 can also help
prevent chronic photoinhibition by maintaining photosynthetic activity, thereby avoiding harmful effects of absorbed light (Nobel 1988). Although chronic photoinhibition is uncommon in cacti, dynamic photoinhibition,
which results in a temporary decrease in photosynthetic
efficiency due to the radiationless dissipation of absorbed
PPF (Taiz and Zeiger 1998), is common, especially during
drought and in the late afternoon under favorable conditions, when endogenous CO2 levels are low (Adams et al.
1989; Barker and Adams 1997). The excess PPF that cannot be used in photosynthesis is dissipated in reactions involving xanthophylls and other carotenoids. For O.
macrorhiza, the concentrations of carotenoids in a cladode surface correlate with orientation, the greatest concentrations occurring in cladodes facing south or west, which
are the surfaces that receive the most sunlight in the afternoon (Barker and Adams 1997).
Influences of plant architecture on the interception of
PPF differ for the massive opaque stems of cacti that exhibit CAM, compared with the relatively thin, flat leaves
of C3 and C4 species, for which light incident on either surface can be distributed throughout the leaf. For instance,
the face of a cladode of a platyopuntia or the side of a
roughly spherical barrel cactus facing poleward can receive
far less than optimal total daily PPF when the opposite
equatorially facing side is approaching PPF saturation of
daily net CO2 uptake. Hence, because some of the plant’s
surfaces face away from direct sunlight essentially the entire day, some face sunward, and others face in intermediate directions, net CO2 uptake by whole plants of such
species under unshaded conditions tends to increase up to
full sunlight. In other words, because of the architecture of
cacti, part of the stems, and hence the plants themselves,
are always light-limited with respect to CO2 uptake.
Moreover, the phototropism of O. leucotricha mentioned
in the Introduction also occurs for O. ficus-indica, as newly
developing cladodes can rotate 16° to become more perpendicular to a unidirectional light beam (Nobel 1982b).
62
Nobel and Bobich
In the field, cladodes tend to be favorably oriented to
intercept more PPF and to have more net CO2 uptake (Fig.
4.2C) and hence more biomass productivity. Such cladode
orientation is dependent on the latitude of the plants and
the timing of cladode initiation. For instance, because of
the path of the sun in the sky, an east-west orientation
tends to be favored for plants close to the equator and decreases with distance from the equator. Cladodes initiated
during the winter tend to have a north-south orientation,
whereas cladodes that are initiated during the summer face
east-west. Because daughter cladodes of various platyopuntias tend to be oriented in the same plane as the underlying mother cladodes, orientation tendencies can be
observed at the whole plant level (Nobel 1982c, 1988).
Nutrients and Salinity
Net CO2 uptake, and thus nocturnal acid accumulation,
for the stems of cacti can also be affected by nutrient levels in the soil and hence in the stem (Nobel 1989). Of the
nutrients investigated, nitrogen has the greatest positive
effect on net CO2 uptake. For O. ficus-indica, nocturnal
net CO2 uptake, as reflected in increases in tissue acidity,
more than doubles as the nitrogen level in the chlorenchyma increases from 1% of the dry mass to over 2% (Nobel
1983). Among nine other species of cacti for which the nitrogen content in the chlorenchyma ranges from 1.0% to
2.5% of the dry mass, maximal nocturnal acid accumulation increases with average nitrogen content (Nobel 1983).
Low stem nitrogen content may be one of the reasons that
Stenocereus queretaroensis has a lower net CO2 uptake than
do most other cultivated cacti (Nobel and PimientaBarrios 1995). For O. engelmannii and O. rastrera, dry mass
increases 73% when fields are fertilized with 160 kg nitrogen per hectare (Nobel et al. 1987). Other nutrients that
show a positive effect on net CO2 uptake by cacti are
potassium, phosphorous, and boron (Nobel et al. 1987;
Nobel 1989).
Soil salinity has a negative affect on net CO2 uptake.
Net CO2 uptake for O. ficus-indica decreases by about 50%
after exposure to a 150 mM NaCl solution and 83% after exposure to a 200 mM solution for 10 weeks (Fig. 4.3). Longer
term exposure to high concentrations of NaCl has an even
more profound effect, with exposure to a solution of 100
mM for six months causing a net CO2 efflux for O. ficusindica (Hatzmann et al. 1991). The concentration of NaCl
in the chlorenchyma also correlates with net CO2 uptake,
because CO2 uptake halves as the sodium level in the
chlorenchyma increases from 20 ppm by dry mass to 300
ppm for O. ficus-indica (Nobel 1983) and from 10 to 80 ppm
for Cereus validus (Nobel et al. 1984). The latter tissue level
daily net co 2 uptake
(fraction of maximum)
ic CO2 concentrations include thicker cladodes, a thicker
chlorenchyma, increased chlorenchyma cell length, a decrease in PEPCase per unit cladode area, a decrease in stem
nitrogen concentration, a decreased stomatal frequency, an
increased root:shoot ratio, increased root cell length, and
a 60 to 70% increase in water-use efficiency (Cui et al.
1993; Luo and Nobel 1993; North et al. 1995; Drennan and
Nobel 2000).
1.0
0.8
0.6
0.4
0.2
0.0
0
50
100
150
200
applied n a c l concentration ( m m)
Figure 4.3. Influence of a 10-week application of NaCl solutions on
daily net CO2 uptake for cladodes of O. ficus-indica. Plants were
grown in 50% Hoagland solution, plus the indicated NaCl concentration. Data are from Nerd et al. (1991).
can be caused by exposing C. validus to 400 mM NaCl for
16 days. When O. humifusa is exposed to 150 mM NaCl for
6 weeks, total daily net CO2 uptake is reduced 94% for
plants from inland populations but 71% for plants from the
marine strand that are exposed to higher soil salinity in their
native habitat (Silverman et al. 1988).Fgiure43n.eahrer:
Atmospheric CO2
As the CO2 concentration in the atmosphere is increased,
the driving force for CO2 entry into cacti, and hence net
CO2 uptake, tends to increase. Yet C3 plants, when exposed to doubled atmospheric CO2 concentrations, often
acclimate to the new conditions after a period of months,
leading to less enhancement than observed initially
(Drennan and Nobel 2000). All four species of cacti that
have been exposed to a doubled atmospheric CO2 concentration have shown increases in both total daily net
CO2 uptake and biomass productivity. For instance, the
biomass increase over a 12-month period is 30% greater for
Ferocactus acanthodes at a doubled compared with the current atmospheric CO2 concentration (Nobel and Hartsock
1986c). For Hylocereus undatus, total daily net CO2 uptake
is 34% higher under the doubled atmospheric CO2 concentration (Raveh et al. 1995), and for Stenocereus queretaroensis it is 36% higher (Nobel 1996).
Opuntia ficus-indica is the most studied CAM species
with respect to effects of elevated atmospheric CO2 concentrations (Drennan and Nobel 2000). Its total daily net
CO2 uptake for terminal cladodes can be enhanced 41 to
61% by doubling the atmospheric CO2 concentration (Cui
et al. 1993). Its biomass increase over a 12-month period is
40% greater under a doubled atmospheric CO2 concentration than under the current one (Nobel and Israel 1994).
Other responses of O. ficus-indica to elevated atmospher-
Net CO2 Uptake: Fruits
CO2 uptake for fruits of cultivated platyopuntias follows a
typical CAM pattern (Fig. 4.1), although maximum instantaneous net CO2 uptake rates and total daily net CO2
uptake (Table 4.2) are only 10 to 40% as high as for stems
(Inglese et al. 1994; Nobel and De la Barrera 2000). In particular, CO2 uptake rates for young fruits of platyopuntias
are similar to those for stems of uncultivated cacti (Table
4.1). The daily net CO2 uptake by young fruits accounts for
only 6 to 15% of their total daily dry-mass gain (Table 4.2).
The contribution to dry-mass gain decreases with fruit age,
reflecting decreases in Rubisco and PEPCase activity and in
chlorophyll content (Inglese et al. 1994). In contrast, during the first two weeks after initiation, cladodes of Opuntia
ficus-indica have a daily net CO2 loss, but at four weeks
they have a net CO2 gain that is similar to that of their underlying mature cladodes (Wang et al. 1997). Thus, cladodes make the transition from a carbohydrate sink to a carbohydrate source at an early age, whereas fruits become
more of a carbohydrate sink as they mature.Tabe4l2.neahrer:
Because the contribution of net CO2 uptake to the
dry-mass gain of platyopuntia fruits is small (Table 4.2),
during their development fruits must receive considerable
carbohydrates from underlying mature cladodes via the
phloem (Inglese et al. 1994; Wang et al. 1997; Nobel and
De la Barrera 2000). However, the importance of the
phloem to fruits lies not only in the supplying of solutes.
Platyopuntia fruits have water potentials that are higher
(less negative) than those of their underlying cladodes, indicating that water cannot flow passively in the xylem from
the cladodes to the fruit (Nobel et al. 1994), as occurs for
most developing organs (Nobel 1999). Rather, the phloem
is responsible for water transport into the fruits of platyopuntias (Nobel et al. 1994), much as it is for 2-week-old
cladodes of O. ficus-indica (Wang et al. 1997). Moreover,
the osmotic pressure of the phloem sap entering the fruits
of O. ficus-indica is relatively low (0.94 MPa) compared
with the phloem sap in other plants (Nobel et al. 1994).
The fruits transpire over 60% of the imported water, leading to a large buildup of solutes (Nobel and De la Barrera
2000). To maintain higher water potentials than their un-
Environmental Biology
63
TA B L E 4 . 2
Net CO2 uptake and its contribution to dry-mass gain for young fruits of six platyopuntia species
Species
Maximum net
CO2 uptake rate
(µmol m –2 s –1)
Total daily net
CO2 uptake
(mmol m –2 day –1)
Percent daily dry mass
gain due to net
CO2 uptake (%)
2.9
4.9
4.8
6.3
4.8
5.9
95
121
85
155
114
174
6.3
10.9
8.8
9.8
8.3
14.8
N. cochenillifera
O. ficus-indica
O. megacantha
O. robusta
O. streptacantha
O. undulata
Data are for fruits midway between floral bud appearance and fruit maturation and are from Nobel and
De la Barrera (2000).
derlying cladodes, the sucrose, other sugars, and amino
acids delivered by the phloem to the fruits (Wang and
Nobel 1995) are probably polymerized into osmotically inactive proteins, mucilage, and starch (Nobel and De la
Barrera 2000). This allows the fruits to continue receiving
water and solutes from the phloem, thus increasing their
sugar and starch content.
Productivity Responses to Environmental Factors
The curves developed for environmental responses of net
CO2 uptake (Fig. 4.2) were obtained in the laboratory,
where one factor at a time was varied under controlled
conditions. However, variations in air temperature,
drought duration, and total daily PPF occur simultaneously in the field. For instance, to examine the effect of elevation on net CO2 uptake and biomass productivity, the
decreasing air temperature, generally increasing water
availability, and variable changes in PPF with increasing elevation must all be considered.
Environmental Productivity Index
One method of dealing with the simultaneous variation of
environmental factors is to multiply the influence of the
three main environmental factors (Fig. 4.2) on net CO2
uptake:
Eq. 4.1 Environmental Productivity Index (EPI) =
Fraction of maximal total daily net
CO2 uptake =
Temperature Index × Water Index ×
PPF Index
where each component index ranges from zero (the point
at which that factor completely inhibits total daily net
CO2 uptake per unit area) to one (where that factor is op-
64
Nobel and Bobich
timal for net CO2 uptake; Nobel 1984, 1988, 1999). Thus,
each component index is normalized to one under the optimal conditions for that environmental factor. EPI can be
used to estimate the fraction of maximal total daily net
CO2 uptake per unit area expected for certain prevailing
conditions and should be calculated for various areas on
the plant. This fraction is multiplied by the total daily net
CO2 uptake per unit area under optimal conditions to predict the actual daily net CO2 uptake. The total daily net
CO2 uptake per plant can be obtained by the actual uptake
values per area times the respective areas into which the
plant has been divided. The total daily net CO2 uptake per
unit ground area can be obtained from the total net CO2
uptake per plant and the ground area per plant. Finally,
CO2 uptake can be numerically converted to biomass, assuming that the CO2 is converted to carbohydrate (30 g
mol-1) or some other biomass equivalent, leading to a biomass productivity in kg m-2 day-1. This daily biomass productivity is then summed up over a year to obtain the annual productivity, which is conventionally expressed in
tons (1,000 kg) dry mass hectare-1 year-1 (Nobel 1991).
Application of EPI to a Barrel Cactus
For Ferocactus acanthodes in the northwestern Sonoran
Desert, the Temperature Index approaches 1.00 for the
moderately cool parts of the year in late autumn and the
spring, as the summer is too hot and the winter is too cold
for maximal net CO2 uptake by this cactus (Fig. 4.4A). The
Water Index approaches 1.00 for the rainy periods in late
summer and the early winter (Fig. 4.4B). The seasonal variation in the PPF Index averaged over the plant surface (Fig.
4.4C) reflects the variation in the sun’s trajectory with the
time of year, being lowest in the winter and highest near the
early summer. The product of these three indices, EPI,
temperature
index
1.0
A
0.5
water index
0.0
1.0
B
0.5
0.0
ppf index
1.0
C
0.5
0.0
D
epi
0.5
0.0
new areole
production
150
E
100
50
0
j
f
m
a
m
j
j
a
s
o
n
d
month
Figure 4.4. Monthly variation in component indices, EPI, and production of new areoles by Ferocactus acanthodes in the northwestern
Sonoran Desert: (A) Temperature Index, (B) Water Index, (C) PPF
Index (including effects of clouds), (D) Environmental Productivity
Index (see Eq. 4.1.), and (E) production of new areoles. Data for the
areoles are from 33 plants examined monthly. Adapted from Nobel
(1986).
reaches a peak in late summer (Fig. 4.4D), which coincides
with the peak in monthly production of new areoles (Fig.
4.4E). The overriding importance of the Water Index is
clearly evident in the seasonal pattern for EPI and areole
production by this cactus in the Sonoran Desert.Fgiure44.neahrere:
Application of EPI to a Cultivated Platyopuntia
Besides relating growth to net CO2 uptake under natural
conditions, EPI can also be used to predict how well a cul-
tivated cactus such as Opuntia ficus-indica may grow in a
particular region (Garcia de Cortázar and Nobel 1991). The
Temperature Index can be used to evaluate maritime or elevational influences on net CO2 uptake for a particular site.
The Water Index can be used to quantify the influence of
the timing of irrigation on net CO2 uptake. However, the
component index that has had the greatest influence on
agronomic practices for O. ficus-indica is the PPF Index,
which can be directly related to plant spacing. In particular, the nonlinear response to total daily PPF for net CO2
uptake by individual stem surfaces (Fig. 4.2C) suggests that
excessive shading should be avoided, i.e., when the stem
area index—total area of both sides of all cladodes per unit
ground area—becomes very high, the average PPF incident
on the cladodes decreases, leading to less total daily net
CO2 uptake per unit cladode area (Fig. 4.5). However, although spacing plants at large distances maximizes net CO2
uptake per plant, it leads to little net CO2 per unit ground
area. Computer models dividing the stems of O. ficusindica into many surfaces facing in different directions and
using hourly values for PPF throughout a year have indicated an optimal spacing for net CO2 uptake per unit
ground area (Garcia de Cortázar et al. 1985; Garcia de
Cortázar and Nobel 1991). In particular, the stem area index
should be about 4 to 5 for optimal biomass productivity
(Fig. 4.5). Although some modification of such close spacing is necessary for management practices, such as picking
of fruit, the simulation modeling indicates that much closer spacing than had been traditionally used in various locations greatly enhances biomass productivity per area.“
Based on calculations using EPI, experimental field
plots were established to ascertain the maximal productivity of O. ficus-indica under environmental conditions approaching those optimal for net CO2 uptake. To meet
those conditions, sites were chosen with moderate nighttime temperatures (and hence a high Temperature Index)
and with nutrient-rich soils. The plants were optimally
spaced or pruned to keep the stem area index at 4 to 5 and
were irrigated to maintain the Water Index near 1.00.
Under such conditions, O. ficus-indica and O. amyclaea
had a annual biomass productivities of 45 to 50 tons dry
mass hectare-1 year-1 (Garcia de Cortazar and Nobel 1992;
Nobel et al. 1992). Such extremely high biomass productivities are exceeded by those of only a few cultivated C3
and C4 species. Indeed, the annual biomass productivities
are 38 tons hectare-1 year-1 for the four most productive C3
crops, 41 tons hectare-1 year-1 for the four most productive
C3 trees, and 56 tons hectare-1 year-1 for the four most productive C4 crops (Nobel 1991). Although most cacti are relatively slow growing, with modest net CO2 uptake, certain
Environmental Biology
65
annual productivity
(tons dry mass hectaare -1 year -1 )
cacti, with their high water-use efficiency, have a distinct advantage over C3 and C4 species in such regions; e.g., O.
ficus-indica can be cultivated in regions with too little soil
moisture for Zea mays and other such crops.
50
Optimal
40
Chile
30
Survival
20
Temperature
Semiarid
10
0
0
2
4
6
8
10
stem area index
(cladode area/ground area)
Figure 4.5. Biomass productivity for O. ficus-indica as a function of
plant spacing as quantified by the stem area index (SAI). Optimal
refers to conditions that include irrigation, leading to a Water Index
of 1.00; Chile refers to natural conditions near Santiago, Chile;
Semiarid refers to conditions that are discussed in the text. Data are
adapted from Garcia de Cortázar et al. (1985); Garcia de Cortázar
and Nobel (1991); and Nobel (1991).
opuntias have great potential for biomass productivity and
could even be used for sequestering massive amounts of
carbon, an international goal for mitigating the increasing
atmospheric CO2 concentrations and the accompanying
global climatic warming.
Besides indicating conditions for maximal productivity,
EPI can also be used to estimate productivity under more
typical field conditions. For instance, predictions can be
made for a region near Santiago, Chile, where O. ficusindica is grown without irrigation for fruit production (Fig.
4.5). Moreover, the stem area index for O. ficus-indica may
be near 2 to allow pathways in the field for plant maintenance and harvesting of fruits or cladodes, which would lead
to 62% of maximal net CO2 uptake per unit ground area
(Fig. 4.5). A site chosen for the cultivation of O. ficus-indica
might not have ideal temperatures, at least for certain seasons. Actually, total daily CO2 uptake does not vary tremendously with mean nighttime temperature, being within
40% of the maximal values for mean nighttime temperatures from 2ºC to 25ºC (Fig. 4.2A), and so the Temperature
Index may average 0.80 on an annual basis. If the cultivation site is semiarid with two wet periods per year, the Water
Index might average 0.25 on an annual basis. Hence, for a
stem area index of 2, EPI may average (0.62)(0.80)(0.25) or
0.12. If 50 tons dry mass hectare-1 year-1 is taken as the maximum productivity, then the predicted productivity for the
site would be (0.12)(50) or 6 tons hectare-1 year-1 (Fig. 4.5),
which is representative of biomass productivities of O. ficusindica in semiarid regions without irrigation. Moreover,
66
Nobel and Bobich
Temperature influences every process in plants, from photosynthesis and respiration to growth and survival. With
respect to survival, low temperatures are more critical with
regard to the distribution of native species of cacti and the
cultivation of opuntias than are high temperatures. For instance, the northern limits of the columnar cacti Carnegiea
gigantea, Lophocereus schottii, and Stenocereus thurberi in
Arizona are dictated by episodic low temperatures (Turnage
and Hinckley 1938; Nobel 1988). In particular, these three
species can be killed by stem temperatures of –7 to –10°C,
similar to the lethal temperatures for cultivated arborescent
species such as Opuntia ficus-indica and Stenocereus queretaroensis (Table 4.3). In contrast, certain lower growing
species can tolerate about –20°C, e. g., Coryphantha vivipara, Opuntia humifusa, and Pediocactus simpsonii (Table
4.3), which are all native to regions in the northern United
States that receive appreciable snowfall. Moreover, Opuntia
fragilis, which occurs north of 56° north latitude in western Canada, can tolerate extremely low stem temperatures
of –48°C (Loik and Nobel 1993).Tabe4l3h.ere:
Cacti that tolerate extremely low temperatures exhibit superior ability for low-temperature hardening (increase
in the tolerance of subzero temperatures as the ambient
temperature is gradually decreased over a period of weeks;
Nobel 1988). Cladodes with lower water content tend to
tolerate lower temperatures (Nobel 1988; Loik and Nobel
1991; Nobel et al. 1995). For example, tissue water content
for O. humifusa (Fig. 4.6), which is native to southeastern
Canada and the eastern United States, generally decreases
by 35% in the winter, and winter kill is limited to plants
with greater water content, suggesting that cold tolerance
for cacti might depend on increases in osmotic pressure
(Koch and Kennedy 1980). In fact, when day/night temperatures decrease from 30/20°C to 10/0°C, the osmotic
pressure for O. humifusa increases four times more than do
the osmotic pressures for the subtropical and less freezingtolerant O. ficus-indica and O. streptacantha, due to a
greater synthesis of simple sugars and the production of
mannitol within the cells of O. humifusa (Goldstein and
Nobel 1994).Fgiure46.here:
Because the cellular contents of cacti would freeze at –1
to –2°C based on their osmotic pressure and the relation
TA B L E 4 . 3
Extreme temperatures that can be tolerated by cacti
Species
Ariocarpus fissuratus
Carnegiea gigantea
Coryphantha vivipara
Denmoza rhodacantha
Epithelantha bokei
Eriosyce ceratistes
Ferocactus acanthodes
F. covillei
F. viridescens
F. wislizenii
Lophocereus schottii
Mammillaria dioica
M. lasiacantha
Opuntia acanthocarpa
O. basilaris
O. bigelovii
O. chlorotica
O. ficus-indica
O. fragilis
O. humifusa
O. polyacantha
O. ramosissima
Pediocactus simpsonii
Stenocereus queretaroensis
S. thurberi
Trichocereus candicans
T. chilensis
Low temperature
tolerance (°C)
High temperature
tolerance (°C)
—
–9
–22
–11
—
–11
–9
–7
–7
–9
–7
—
—
—
—
–8
—
–10
–48
–25
–18
–5
–19
–9
–10
–9
–9
70
65
68
—
64
—
69
71
69
70
68
69
67
65
66
64
69
70
—
—
—
68
—
—
68
—
—
Data are based on the uptake of a vital dye (neutral red) by cells in tissues exposed
to the extreme temperature for 1 hour for plants that have been gradually adjusted
to low or high day/night air temperatures. The indicated temperature will cause
immediate death of over half of the cells, generally leading to death of the plants.
Data are from Nobel (1982a, 1988, 1996); Smith et al. (1984); Nobel et al. (1986);
and Loik and Nobel (1993).
between freezing-point depression and osmotic pressure
(Nobel 1999), the mechanism of low temperature damage
to cacti is not due to the wholesale freezing of cellular water
(Nobel 1988). Rather, the initial ice crystals form outside
the cells, following a super cooling of the stem (lowering
of the stem temperature below the freezing point of the cell
sap). Intracellular water molecules are then progressively
transferred to the growing extracellular crystals, leading to
the shrinkage of protoplasts and the desiccation of the
cells. The continuing dehydration of the cells affects their
membranes and enzymes, leading to disruption of metabolic processes and eventual cellular death.
How many genes are involved in the multitude of factors leading to greater tolerance of low temperature? This
is an important question for breeders and biotechnologists
(Chapter 15) dealing with commercialized cacti, as domesticated cultivars tend to have a poorer tolerance of low
temperatures compared with many native species (Russell
and Felker 1987; Table 4.3). Indeed, one of the major limitations in the expansion of the regions where O. ficusindica and other platyopuntias can be cultivated is episodic low temperatures, suggesting that global climate
warming will be favorable to increased cultivation of such
cacti (Nobel 1996).
Environmental Biology
67
Figure 4.6. Opuntia humifusa on a snow-covered hillside in Ontario,
Canada. The wrinkled appearance of the cladodes is due to dehydration, a response to exposure to low temperatures. Photograph is
courtesy of Michael E. Loik.
Although low temperature tolerance has major effects
on the natural distribution of cacti and the regions where
they can be successfully cultivated, high temperatures are
generally not a major limiting factor. For instance, of the
17 species that have been assessed quantitatively, the tolerated high temperature averages 68°C (Table 4.3). This is an
incredibly high temperature to survive, as metabolic
processes are severely disrupted at 55 to 60°C for most
plants (Nobel 1988). Tolerated high temperatures are remarkably similar among stem types, being essentially the
same for barrel cacti, columnar cacti, and opuntias (Table
4.3). The cellular and genetic bases for the high-temperature tolerance of cacti have not been described. In any case,
high-temperature damage generally occurs for cacti where
the stem contacts the soil, which can have surface temperatures of up to 70ºC in deserts (Nobel 1988). Hence nurse
plants are often important for ensuring the establishment
of cactus seedlings in nature, and care must be exercised in
the seasonal timing for planting young plants or cladodes
of cultivated species to avoid stem overheating.
Water
Related to the tolerance by cacti of cellular desiccation during subzero episodes (Fig. 4.6) is the ability of cacti to
withstand dehydration caused by drought. For O. acanthocarpa, O. basilaris, and O. bigelovii, uprooted plants can
survive 3 years without water (Szarek and Ting 1975;
Smith and Madhaven 1982). Moreover, Copiapoa cinerea
can survive outdoors (Gulmon et al. 1979) and Ferocactus
68
Nobel and Bobich
wislizenii indoors (MacDougal et al. 1915) for 6 years without water. Carnegiea gigantea, F. acanthodes, and O. basilaris can lose 80% of their stem water and still survive
(Barcikowski and Nobel 1984), and Coryphantha vivipara
can lose 91% of its stem water when exposed to drought
and live (Nobel 1981). Indeed, the ability of cacti to tolerate cellular water loss, and hence drought, is correlated
with their ability to tolerate intracellular water loss during
subzero temperature episodes accompanied by extracellular ice formation.
Cacti can store an immense amount of water in their
succulent stems. This has ramifications at the tissue level;
during drought, water is shuttled from the internal whitish
water-storage parenchyma to the greenish photosynthetic
chlorenchyma, thereby allowing net CO2 uptake to proceed for extended periods. For instance, the water-storage
parenchyma in Carnegiea gigantea and Ferocactus acanthodes loses four times more water than does their chlorenchyma during drought (Barcikowski and Nobel 1984).
Similar patterns of water loss are also exhibited by O. basilaris and O. ficus-indica (Barcikowski and Nobel 1984;
Goldstein et al. 1991). Likewise, the water storage parenchyma loses more water than does the chlorenchyma in O.
humifusa in response to cooling from day/night temperatures of 25°/15°C to 5°/–5°C (Loik and Nobel 1991).
Internal water redistribution reflects differences in the
cellular properties of water-storage parenchyma and chlorenchyma. For C. gigantea and F. acanthodes exposed to
drought, solutes are lost from the water storage parenchyma, thus creating a difference in the osmotic pressure
between the two tissues and facilitating water movement
into the chlorenchyma (Barcikowski and Nobel 1984).
When O. ficus-indica is exposed to drought, the osmotic
pressure in the water storage parenchyma becomes lower
than in the chlorenchyma due to a polymerization of sugars leading to the formation of starch grains in the waterstorage parenchyma (Goldstein et al. 1991). Water-storage
parenchyma can also survive at a lower relative water content than chlorenchyma. Mucilage, which has a very high
water-binding capacity, also occurs in greater amounts in
the intercellular air spaces of water storage tissue than in
the chlorenchyma (Goldstein et al. 1991). In addition, the
elastic modulus of the cell walls of water storage parenchyma is only 40% of that of the chlorenchyma, allowing the
water-storage parenchyma to maintain turgor over a large
range of water contents.
Other aspects of water conservation and drought tolerance by cacti include relatively low stem stomatal frequencies of 20 to 80 per mm2 compared with 100 to 300
per mm2 for leaves of C3 and C4 species (Conde 1975;
Another factor that can be lethal for cacti is soil salinity.
Indeed, cacti generally do not thrive in soils that are high
in sodium chloride or calcium carbonate, which affects native populations and where opuntias can be cultivated.
Cereus validus is native to the salt flats of Salinas Grandes,
Argentina; this area experiences high salinity during the
dry season, when its root system withers as water evaporates and salts collect near the soil surface (Nobel 1988).
During the dry season, the sodium concentration increases in its roots and is progressively lower toward the apex of
the stem (Nobel et al. 1984), as also occurs for O. ficusindica (Berry and Nobel 1985). The increasing concentration of NaCl in the chlorenchyma of these two species as
the soil salinity increases is accompanied by a decrease in
net CO2 uptake, as also occurs for O. humifusa (Silverman
et al. 1988), and can eventually lead to plant death
Inhibition of growth for O. ficus-indica is approximately linear with soil sodium content, with 150 ppm by
mass of Na leading to approximately 50% inhibition of
shoot growth (Nobel 1989). A similar 50% inhibition of
shoot growth can be caused by watering with 60 mM
NaCl (12% of the salinity of seawater) for 6 months, which
leads to 84% inhibition of root growth (Berry and Nobel
1985). Plant dry weight for O. ficus-indica is 60% less for
plants irrigated with 200 mM NaCl compared with 5 mM
NaCl (Nerd et al. 1991). Cladode water content is also 10%
lower, and cladode osmotic pressure doubles for plants
under the higher NaCl regime. For Ferocactus acanthodes,
watering with 60 mM NaCl has relatively little effect on
existing shoot biomass but reduces existing root biomass
by 40% (Berry and Nobel 1985), again indicating that root
growth is more sensitive to salinity than is shoot growth.
The wood of the columnar cactus Carnegiea gigantea may
get stiffer with plant age, making the stem resist deflection
and buckling more per amount of wood area and allowing
this species to reach heights of 15 m (Niklas and Buchman
1994). Also, stems of another columnar cactus, Pachycereus
pringlei, are able to resist bending moments due to xylem
accumulation being greater at the base of the plant, much
in the same manner as typical dicotyledonous trees (Niklas
et al. 1999). The ribs of this species also provide support,
especially for young plants and younger tissue on older
plants.
Branches of platyopuntias are able to resist applied
forces with little deflection or deformation, even though
the cladodes of a branch are connected by a junction that
is usually small in cross-sectional area compared to the
middle of the individual segments. For instance, for a
cladode of O. ficus-indica that is 30 cm in length, the angular deflection of the junction with the underlying cladode, plus the cladode itself, is 6° when loaded by a force
equal to the cladode mass perpendicular to the face of the
cladode at the center of mass, 2° when the applied mass is
parallel to the cladode’s face, and 2° for a relatively high
windspeed of 10 m s-1 (36 km hour-1; Nobel and Meyer
1991). Thus, even though cladodes are thin compared with
stems of many woody plants and the cladode junctions are
relatively small in area compared with other regions of the
branch, the shoots of O. ficus-indica are quite rigid.
For other cacti, stem failure under static or dynamic
loading may actually be advantageous because it results in
vegetative reproduction. It is believed that when the semierect shrub Stenocereus gummosus reaches its structural
height limit, the failure of the stems leads to vegetative reproduction because the stems root after they come into
contact with the ground, eventually forming dense
colonies (Molina-Freaner et al. 1998). A hybrid platyopuntia in southern California also exhibits this type of
vegetative reproduction (Bobich and Nobel 2001). In particular, the cladode junctions of the hybrid O. “occidentalis” are weaker than the junctions of either of its putative
parent species, O. ficus-indica and O. littoralis, allowing O.
“occidentalis” to form large thickets. These thickets are also
large enough to survive fires that engulf isolated plants of
O. ficus-indica and O. littoralis, thus giving O. “occidentalis” a selective advantage over its parent species in a chaparral region subject to periodic fires.
Gravity and Wind
Conclusions and Future Prospects
Due to their mechanical strength, imparted primarily by
the wood content in their stems (Gibson and Nobel
1986), cacti do not deflect greatly due to gravity or wind.
Since the early research performed at the Desert Botanical
Laboratory, information on the environmental biology of
cacti has grown substantially. Interest in the physiology of
Pimienta-Barrios et al. 1993; Nobel 1994). Therefore, only
a small fraction of the surface area of cacti is available for
water loss to the atmosphere. Also, the waxy cuticles covering the stems tend to be relatively thick for cacti—5 to 30
µm — compared with cuticles that are only 0.4 to 2 µm
thick on the leaves of representative C3 and C4 species
(Conde 1975; Pimienta-Barrios et al. 1993; North et al.
1995). As another adaptation, cacti have shallow roots, with
a mean depth of only 10 to 15 cm, which facilitates responses to light rainfall; in addition, new roots develop
rapidly once the soil is wet (Nobel 1988).
Salinity
Environmental Biology
69
cacti is spurred by their extraordinary ability to survive
high temperatures, high PPF, and especially drought. The
utilization of CAM by most of the species in the family in
coping with these environmental stresses has added to the
interest in these plants and has led to investigations of net
CO2 uptake for species from all three subfamilies, as well
as knowledge of water-use efficiency and productivity for
select species. However, net CO2 uptake and productivity
have been investigated for only slightly more than 2% of
the species in the family. Also, the environmental biology
of cacti from certain regions, such as the Atacama Desert
of Chile, is lacking, suggesting that much more field research is needed.
As ecological and agricultural interest in cacti grows,
greater insight into their optimal growth conditions will
become essential. This will require detailed analyses of the
effects that temperature, light, and water have on CO2 uptake for particular species. Greater attention should also be
paid to how mineral nutrition affects net CO2 uptake, because soil elements other than sodium have been studied
only for their effects on nocturnal acid accumulation
(Nobel 1983) or on productivity (Nobel et al. 1987; Nobel
1989). Moreover, the optimal soil properties, such as the
sand, silt, and clay fractions, should be further investigated because some species have the ability to grow on various types of soil while others are restricted to special soil
types (Benson 1982).
Although comparisons of net CO2 uptake and productivity have been made between crops and certain cacti,
particularly the highly productive O. ficus-indica (Nobel,
1988), comparisons of uncultivated cacti with sympatric
species in their native habitat are rare. For example,
Opuntia humifusa has received attention because it is the
most widespread cactus in North America and is exposed
to many different environmental stresses (Silverman et al.
1988; Loik and Nobel 1991). Yet, little is known about the
net CO2 uptake for O. humifusa compared to that of the
many species with which it coexists or about which characteristics allow this species to compete successfully for resources in such a wide variety of habitats. Similarly, little
is known about the productivity for most uncultivated epiphytic cacti, even though over 10% of the Cactaceae are
epiphytes (Gibson and Nobel 1986). With the large
amount of information on the utilization of CAM by other
vascular epiphytes (Griffiths 1989; Zotz and Ziegler 1997),
studies comparing their CO2 uptake to that of epiphytic
cacti in response to various environmental factors would
aid in understanding the distribution and frequency of
epiphytic cacti in the canopies of tropical forests.
New research possibilities for the environmental biol-
70
Nobel and Bobich
ogy of cacti go far beyond the aforementioned topics.
Improvements in instrumentation will facilitate investigations of the photosynthetic strategies for cacti and their
ability to survive harsh conditions. Moreover, members of
the Cactaceae occur natively from southern Argentina and
Chile to Canada, and from coastal strand communities to
tropical alpine environments, and are cultivated in more
than 30 countries (Gibson and Nobel 1986; Nobel 1994).
Almost endless possibilities exist for interesting environmental studies involving cacti.
Literature Cited
Adams, W. W., III, M. Diaz, and K. Winter. 1989. Diurnal
changes in photochemical efficiency, the reduction
state of Q, radiationless energy dissipation, and nonphotochemical fluorescence quenching in cacti exposed to natural sunlight in northern Venezuela.
Oecologia 80: 553–561.
Barcikowski, W., and P. S. Nobel. 1984. Water relations of
cacti during desiccation: distribution of water in tissues. Botanical Gazette 145: 110–115.
Barker, D. H., and W. W. Adams III. 1997. The xanthophyll cycle and energy dissipation in differently oriented faces of the cactus Opuntia macrorhiza. Oecologia
109: 353–361.
Benson, L. 1982. The Cacti of the United States and Canada.
Stanford University Press, Stanford, California.
Berry, W. L., and P. S. Nobel. 1985. Influence of soil and
mineral stresses on cacti. Journal of Plant Nutrition 8:
679–696.
Bobich, E. G., and P. S. Nobel. 2001. Biomechanics and
anatomy of cladode junctions for two Opuntia (Cactaceae) species and their hybrid. American Journal of
Botany 88: 391–400.
Cannon, W. A. 1925. Physiological Features of Roots, with
Especial Reference to the Relation of Roots to Aeration of
the Soil. Publication 368, Carnegie Institution of
Washington, Washington, D.C.
Conde, L. F. 1975. Anatomical comparisons of five species
of Opuntia (Cactaceae). Annals of the Missouri Botanical Garden 62: 425–473.
Cui, M., P. M. Miller, and P. S. Nobel. 1993. CO2 exchange
and growth of the Crassulacean acid metabolism plant
Opuntia ficus-indica under elevated CO2 in open-top
chambers. Plant Physiology 103: 519–524.
Cui, M., and P. S. Nobel. 1994. Gas exchange and growth
responses to elevated CO2 and light levels for the
CAM species Opuntia ficus-indica. Plant, Cell and
Environment 17: 935–944.
Drennan, P. M., and P. S. Nobel. 2000. Response of
CAM species to increasing atmospheric CO2 concentrations. Plant, Cell and Environment 23: 767–781.
Franco-Vizcaíno, E., G. Goldstein, and I. P. Ting. 1990.
Comparative gas exchange of leaves and bark in three
stem succulents of Baja California. American Journal of
Botany 77: 1272–1278.
Garcia de Cortázar, V., and P. S. Nobel. 1991. Prediction
and measurement of high annual productivity for
Opuntia ficus-indica. Agricultural and Forest Meteorology 56: 261–272.
Garcia de Cortázar, V., and P. S. Nobel. 1992. Biomass and
fruit production for the prickly pear cactus, Opuntia
ficus-indica. Journal of the American Society for Horticultural Science 117: 558–562.
Garcia de Cortázar, V., E. Acevedo, and P. S. Nobel. 1985.
Modeling of PAR interception and productivity by
Opuntia ficus-indica. Agricultural and Forest Meteorology 34: 145–162.
Gerwick, B. C., and G. J. Williams III. 1978. Temperature
and water relations of gas exchange of Opuntia polyacantha. Oecologia 35: 149–159.
Gibson, A. C., and P. S. Nobel. 1986. The Cactus Primer.
Harvard University Press, Cambridge, Massachusetts.
Goebel, K. 1895. Über die Einwirkung des Lichtes auf die
Gestaltung der Kakteen und anderer Pflanzen. Flora
80: 96–116.
Goldstein, G., J. L. Andrade, and P. S. Nobel. 1991.
Differences in water relations parameters for the
chlorenchyma and the parenchyma of Opuntia ficusindica under wet versus dry conditions. Australian
Journal of Plant Physiology 18: 95–107.
Goldstein, G., and P. S. Nobel. 1994. Water relations and
low-temperature acclimation for cactus species varying
in freezing tolerance. Plant Physiology 104: 675–681.
Griffiths, D. 1915. Yields of Native Prickly Pear in Southern
Texas. Bulletin 208, United States Department of
Agriculture, Washington, D.C.
Griffiths, H. 1989. Carbon dioxide concentrating mechanisms and the evolution of CAM in vascular epiphytes.
In Vascular Plants as Epiphytes (U. Lüttge, ed.).
Springer-Verlag, Berlin. Pp. 42–81.
Gulmon, S. L., P. W. Rundel, and J. R. Ehleringer. 1979.
Spatial relationships and competition in a Chilean
desert cactus. Oecologia 44: 40–43.
Hanscom, Z., III, and I. P. Ting. 1978. Irrigation magnifies
CAM-photosynthesis in Opuntia basilaris (Cactaceae).
Oecologia 33: 1–15.
Hatzmann, S., G. Ebert, and P. Lüdders. 1991. Influence of
NaCl salinity on growth, ion uptake, and gas exchange
of Opuntia ficus-indica (L.) Miller. Angewandte Botanik
65: 161–168.
Inglese, P., A. A. Israel, and P. S. Nobel. 1994. Growth and
CO2 uptake for cladodes and fruit of the Crassulacean
acid metabolism species Opuntia ficus-indica during
fruit development. Physiologia Plantarum 91: 708–714.
Israel, A. A., and P. S. Nobel. 1995. Growth temperature
versus CO2 uptake, Rubisco and PEPCase activities,
and enzyme high-temperature sensitivities for a CAM
plant. Plant Physiology and Biochemistry 33: 345–351.
Kluge, M., and I. P. Ting. 1984. Crassulacean Acid
Metabolism: Analysis of an Ecological Adaptation.
Springer-Verlag, Berlin.
Koch, K. E., and R. A. Kennedy. 1980. Effects of seasonal
changes in the Midwest on Crassulacean acid metabolism (CAM) in Opuntia humifusa Raf. Oecologia 45:
390–395.
Littlejohn, R. O., Jr. 1983. Environmental regulation of
photosynthesis and characterization of phase II
Crassulacean acid metabolism in three Opuntia species.
Ph.D. Thesis, Washington State University, Pullman.
Loik, M. E., and P. S. Nobel. 1991. Water relations and
mucopolysaccharide increases for a winter hardy cactus
during acclimation to subzero temperatures. Oecologia
88: 340–346.
Loik, M. E., and P. S. Nobel. 1993. Freezing tolerance and
water relations of Opuntia fragilis from Canada and the
United States. Ecology 74: 1722–1732.
Long, E. R. 1915. Acid accumulation and destruction in
large succulents. The Plant World 18: 261–272.
Luo, Y., and P. S. Nobel. 1993. Growth characteristics of
newly initiated cladodes of Opuntia ficus-indica as
affected by shading, drought, and elevated CO2. Physiologia Plantarum 87: 467–474.
Lüttge, U., and P. S. Nobel. 1984. Day-night variations in
malate concentration, osmotic pressure, and hydrostatic pressure in Cereus validus. Plant Physiology 75:
804–807.
MacDougal, D. T. 1924. Growth in Trees and Massive
Organs of Plants—Dendrographic Measurements. Publication 350, Carnegie Institution of Washington,
Washington, D.C.
Environmental Biology
71
MacDougal, D. T., and W. A. Cannon. 1910. The Conditions of Parasitism in Plants. Publication 129, Carnegie
Institution of Washington, Washington, D.C.
nocturnal acidity increases for terminal cladodes of a
widely cultivated cactus, Opuntia ficus-indica. American Journal of Botany 69: 1462–1469.
MacDougal, D. T., E. R. Long, and J. G. Brown. 1915. End
results of desiccation and respiration in succulent
plants. Physiological Researches 1: 289–325.
Nobel, P. S. 1982c. Orientations of terminal cladodes of
platyopuntias. Botanical Gazette 143: 219–224.
MacDougal, D. T., and E. B. Working. 1922. A new hightemperature record for growth. Carnegie Institution of
Washington Year Book 20: 47–48.
MacDougal, D. T., and E. B. Working. 1933. The Pneumonic System of Plants, Especially Trees. Publication 441,
Carnegie Institution of Washington, Washington,
D.C.
McGinnies, W. C. 1981. Discovering the Desert: Legacy of the
Carnegie Desert Botanical Laboratory. University of
Arizona Press, Tucson.
Mizrahi, Y., A. Nerd, and P. S. Nobel. 1997. Cacti as crops.
Horticultural Reviews 18: 291–319.
Molina-Freaner, F., C. Tinoco-Ojanguren, and K. J.
Niklas. 1998. Stem biomechanics of three columnar
cacti from the Sonoran Desert. American Journal of
Botany 85: 1082–1090.
Nerd, A., A. Karadi, and Y. Mizrahi. 1991. Salt tolerance of
prickly pear cactus (Opuntia ficus-indica). Plant and
Soil 137: 201–207.
Niklas, K. J., and S. L. Buchman. 1994. The allometry of
saguaro height. American Journal of Botany 81: 1161–
1168.
Niklas, K. J., F. Molina-Freaner, and C. TinocoOjanguren. 1999. Biomechanics of the columnar cactus Pachycereus pringlei. American Journal of Botany 86:
767–775.
Nisbet, R. A., and D. T. Patten. 1974. Seasonal temperature acclimation of a prickly-pear cactus in south-central Arizona. Oecologia 15: 345–352.
Nobel, P. S. 1978. Surface temperatures of cacti—influences
of environmental and morphological factors. Ecology
59: 986–996.
Nobel, P. S. 1980. Influences of minimum stem temperatures on ranges of cacti in southwestern United States
and central Chile. Oecologia 47: 10–15.
Nobel, P. S. 1981. Influence of freezing temperatures on
a cactus, Coryphantha vivipara. Oecologia 48: 194–
198.
Nobel, P. S. 1982a. Low temperature tolerance and cold
hardening of cacti. Ecology 63: 1650–1656.
Nobel, P. S. 1982b. Orientation, PAR interception, and
72
Nobel and Bobich
Nobel, P. S. 1983. Nutrient levels in cacti—relation to nocturnal acid accumulation and growth. American
Journal of Botany 70: 1244–1253.
Nobel, P. S. 1984. Productivity of Agave deserti: measurement by dry weight and monthly prediction using
physiological responses to environmental parameters.
Oecologia 64: 1–7.
Nobel, P. S. 1986. Relation between monthly growth of
Ferocactus acanthodes and an environmental productivity index. American Journal of Botany 73: 541–547.
Nobel, P. S. 1988. Environmental Biology of Agaves and
Cacti. Cambridge University Press, New York.
Nobel, P. S. 1989. A nutrient index quantifying productivity of agaves and cacti. Journal of Applied Ecology 26:
635–645.
Nobel, P. S. 1991. Tansley Review No. 32. Achievable productivities of certain CAM plants: Basis for high values compared with C3 and C4 plants. New Phytologist
119: 183–205.
Nobel, P. S. 1994. Remarkable Agaves and Cacti. Oxford
University Press, New York.
Nobel, P. S. 1996. Responses of some North American
CAM plants to freezing temperatures and doubled
CO2 concentrations: Implications of global climate
change for extending cultivation. Journal of Arid
Environments 34: 187–196.
Nobel, P. S. 1999. Physiochemical and Environmental
Plant Physiology, 2nd ed. Academic Press, San Diego,
California.
Nobel, P. S., J. L. Andrade, N. Wang, and G. B. North.
1994. Water potentials for developing cladodes and
fruits of a succulent plant, including xylem-versusphloem implications for water movement. Journal of
Experimental Botany 45: 1801–1807.
Nobel, P. S., and E. De la Barrera. 2000. Carbon and water
balances for young fruits of platyopuntias. Physiologia
Plantarum 109: 160–166.
Nobel, P. S., E. Garcia-Moya, and E. Quero. 1992. High
annual productivity of certain agaves and cacti under
cultivation. Plant, Cell and Environment 15: 329–335.
Nobel, P. S., G. N. Geller, S. C. Kee, and A. D. Zimmerman. 1986. Temperatures and thermal tolerances for
cacti exposed to high temperatures near the soil surface. Plant, Cell and Environment 9: 279–287.
Nobel, P. S., and T. L. Hartsock. 1983. Relationships between photosynthetically active radiation, nocturnal
acid accumulation, and CO2 uptake for a Crassulacean
acid metabolism plant, Opuntia ficus-indica. Plant
Physiology 71: 71–75.
Nobel, P. S., and T. L. Hartsock. 1984. Physiological responses of Opuntia ficus-indica to growth temperature.
Physiologia Plantarum 60: 98–105.
Nobel, P. S., and E. Pimienta-Barrios. 1995. Monthly stem
elongation for Stenocereus queretaroensis: Relationships
to environmental conditions, net CO2 uptake and seasonal variations in sugar content. Environmental and
Experimental Botany 35: 17–24.
Nobel, P. S., C. E. Russell, P. Felker, J. G. Medina, and E.
Acuña. 1987. Nutrient relations and productivity of
prickly pear cacti. Agronomy Journal 79: 550–555.
Nobel, P. S., and T. L. Hartsock. 1986a. Leaf and stem CO2
uptake in the three subfamilies of the Cactaceae. Plant
Physiology 80: 913–917.
Nobel, P. S., N. Wang, R. A. Balsamo, M. E. Loik, and
M. A. Hawke. 1995. Low-temperature tolerance and
acclimation of Opuntia spp. after injection of glucose
or methylglucose. International Journal of Plant
Sciences 156: 496–504.
Nobel, P. S., and T. L. Hartsock. 1986b. Environmental
influences on the productivity of three desert succulents in the south-western United States. Plant, Cell
and Environment 9: 741–749.
North, G. B., T. L. Moore, and P. S. Nobel. 1995. Cladode
development for Opuntia ficus-indica (Cactaceae)
under current and doubled CO2 concentrations.
American Journal of Botany 82: 159–166.
Nobel, P. S., and T. L. Hartsock. 1986c. Short-term and
long-term responses of Crassulacean acid metabolism
plants to elevated CO2. Plant Physiology 82: 604–606.
Osmond, C. B. 1978. Crassulacean acid metabolism: A curiosity in context. Annual Review of Plant Physiology 29:
379–414.
Nobel, P. S., and T. L. Hartsock. 1987. Drought-induced
shifts in daily CO2 uptake patterns for leafy cacti.
Physiologia Plantarum 70: 114–118.
Osmond, C. B., M. M. Ludlow, R. Davis, I. R. Cowan,
S. B. Powles, and K. Winter. 1979. Stomatal responses
to humidity in Opuntia inermis in relation to control
of CO2 and H2O exchange patterns. Oecologia 41: 65–
76.
Nobel, P. S., and T. L. Hartsock. 1990. Diel patterns of
CO2 exchange for epiphytic cacti differing in succulence. Physiologia Plantarum 78: 628–634.
Nobel, P. S., and A. A. Israel. 1994. Cladode development,
environmental responses of CO2 uptake, and productivity for Opuntia ficus-indica under elevated CO2.
Journal of Experimental Botany 45: 295–303.
Nobel, P. S., and M. E. Loik. 1990. Thermal analysis, cell
viability, and CO2 uptake of a widely distributed
North American cactus, Opuntia humifusa, at subzero
temperatures. Plant Physiology and Biochemistry 28:
429–436.
Nobel, P. S., M. E. Loik, and R. W. Meyer. 1991.
Microhabitat and diel tissue acidity changes for two
sympatric cactus species differing in growth habit.
Journal of Ecology 79: 167–182.
Nobel, P. S., U. Lüttge, S. Heuer, and E. Ball. 1984.
Influence of applied NaCl on Crassulacean acid metabolism and ionic levels in a cactus, Cereus validus.
Plant Physiology 75: 799–803.
Nobel, P. S., and R. W. Meyer. 1991. Biomechanics of
cladodes and cladode-cladode junctions for Opuntia
ficus-indica (Cactaceae). American Journal of Botany 78:
1252–1259.
Pimienta-Barrios, E., M. Luera-Quezada, and L. L. Lopez
Amezcua. 1993. Estudio anatómico comparativo en
colectas del subgenero Opuntia. Agrociencia, Series
Fitociencia 4: 7–14.
Pimienta-Barrios, E., and P. S. Nobel. 1998. Vegetative, reproductive, and physiological adaptations to aridity of
pitayo (Stenocereus queretaroensis, Cactaceae). Economic
Botany 52: 401–411.
Raveh, E., M. Gersani, and P. S. Nobel. 1995. CO2 uptake
and fluorescence responses for a shade-tolerant cactus
Hylocereus undatus under current and doubled CO2
concentrations. Physiologia Plantarum 93: 505–511.
Richards, H. M. 1915. Acidity and Gas Interchange in Cacti.
Publication 209, Carnegie Institution of Washington,
Washington, D.C.
Russell, C. E. and P. Felker. 1987. Comparative cold hardiness of Opuntia spp. and cvs. grown for fruit, vegetable, and fodder production. Journal of Horticultural
Science 2: 545–550.
Shreve, F. 1931. The Cactus and Its Home. Williams and
Wilkins, Baltimore.
Silverman, F. P., D. R. Young, and P. S. Nobel. 1988.
Environmental Biology
73
Effects of applied NaCl on Opuntia humifusa.
Physiologia Plantarum 72: 343–348.
Smith, B. N., and S. Madhaven. 1982. Carbon isotope ratios in obligate and facultative CAM plants. In
Crassulacean Acid Metabolism (I. P. Ting and M. Gibbs,
eds.). American Society of Plant Physiologists,
Rockville, Maryland. Pp. 231–243.
Smith, S. D., B. Didden-Zopfy, and P. S. Nobel. 1984.
High-temperature responses of North American cacti.
Ecology 65: 643–651.
Szarek, S. R., and I. P. Ting. 1975. Photosynthetic efficiency
of CAM plants in relation to C3 and C4 plants. In
Environmental and Biological Control of Photosynthesis
(R. Marcelle, ed.). Dr. W. Junk, The Hague. Pp. 289–
297.
Taiz, L., and E. Zeiger. 1998. Plant Physiology, 2nd ed.
Sinauer Associates, Sunderland, Massachusetts.
Turnage, W. V., and A. L. Hinckley. 1938. Freezing weather in relation to plant distribution in the Sonoran
Desert. Ecological Monographs 8: 529–550.
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Nobel and Bobich
Troughton, J. H., P. V. Wells, and H. A. Mooney. 1974.
Photosynthetic mechanisms in ancient C4 and CAM
species. Carnegie Institution Year Book 73: 812–816.
Uphof, J. C. Th. 1916. Cold-Resistance in Spineless Cacti.
Bulletin 79, University of Arizona Agricultural
Experiment Station, Tucson.
Wang, N., and P. S. Nobel. 1995. Phloem exudate collected via scale insect stylets for the CAM species Opuntia
ficus-indica under current and doubled CO2 concentrations. Annals of Botany 75: 525–532.
Wang, N., H. Zhang, and P. S. Nobel. 1997. Phloemxylem water flow in developing cladodes of Opuntia
ficus-indica during sink-to-source transition. Journal of
Experimental Botany 48: 675–682.
Zotz, G., and H. Ziegler. 1997. The occurrence of
Crassulacean acid metabolism among vascular epiphytes from Central Panama. New Phytologist 137: 223–
229.
CHAPTER
›5‹
R E P RO D U C T I V E B I O LO G Y
Eulogio Pimienta-Barrios and Rafael F. del Castillo
Introduction
Areoles and Reproductive Structures
Flower Types, Pollinators, and Pollination
Outcrossing Mechanisms
Polyploidy and Reproduction
Flower Fertilization
Flower and Fruit Development
Seed Germination
Apomixis
Conclusions
Literature Cited
Introduction
Cacti are native to the Americas, where they are widely distributed. More than 70% of the species occur in arid and
semiarid regions of Mexico, Peru, Argentina, and Chile
(Gibson and Nobel 1986; Arias-Moreno 1997). Water is the
major limiting factor for plant productivity in such environments (Fischer and Turner 1978), although other factors, such as strong drying winds, extreme temperatures,
limited nutrients, and high light intensity, can exacerbate
the effects of water scarcity (Berry et al. 1983). Cacti perhaps provide the best examples of adaptations to aridity, including morphological modifications (e.g., succulence,
low stomatal density, thick cuticles) and Crassulacean acid
metabolism (Gibson and Nobel 1986; Nobel 1995).
However, with the exception of seed germination and establishment (Rójas-Aréchiga and Vázquez-Yanes 2000),
the role of most of the reproductive adaptations of cacti to
aridity has been poorly studied and comes mainly from ex-
periments with cultivated plants (Pimienta-Barrios and
Nobel 1998). Nevertheless, studies on reproductive development for cacti in arid environments can help explain the
evolution of reproductive mechanisms in cacti, which
allow them to cope with the prevailing physical and chemical stresses. This review describes the available information
on reproductive biology, relating various aspects to the
adaptations of cacti to aridity and to the origin and causes of biological variation.
Areoles and Reproductive Structures
The distinctive vegetative structure of cacti is the areole, considered homologous to a lateral (axillary) bud
(Buxbaum 1950). Hairs, foliar organs, reproductive organs,
glochids, and roots develop from the areoles (Booke 1980).
Cactus flowers are sessile and solitary, and commonly only
one flower is produced per areole, which helps increase
fruit set for cacti (Ramírez and Berry 1995). However, several flowers are sometimes derived from a single areole,
75
e.g., for Pterocereus foetidus (Bravo-Hollis 1978). For
Myrtillocactus, several areoles join to produce an inflorescence in which the flowers appear to be derived from a
single meristem (Buxbaum 1950). For Opuntia prolifera, a
chain of fruits results from the development of flowering
buds derived from pericarpic areoles. The flowers are usually campanulate with radial symmetry (actinomorphic),
but in epiphytic species the flowers are lightly bilateral (zygomorphic), as the stamens and styles are located in the
ventral part of the flower (Arreola-Nava 1997). The large,
attractive flowers for which cacti are noted occur in most
self-fertile polyploids (Ross 1981). For instance, the flowers
of Opuntia are large, and mature flowers can vary from 6
to 10 cm in length; they commonly are borne near the apex
of the cladodes (Gibson and Nobel 1986). The flowers of
Cereus peruvianus, Hylocereus spp., and Selenicereus megalanthus are among the largest in the cactus family (Nerd
and Mizrahi 1997).
Cactus flowers usually open and close during the same
day. For opuntias (Fig. 5.1), flower opening typically lasts
8 to 11 hours (Rosas and Pimienta 1986; del Castillo and
González-Espinosa 1988; Osborn et al. 1988; Mandujano et
al. 1996), but some flowers may open a second day (Grant
and Grant 1981; Rosas and Pimienta 1986). Ephemeral
flowers are common in dry tropical rainforests; this flower
behavior apparently decreases water loss by decreasing the
time available for transpiration (Primack 1982).Fgiuren51.eahrere:
The most fundamental characteristic of a cactus flower
is its inferior ovary, meaning that the ovary occurs below
the perianth parts and the stamens (Fig. 5.2); only a few
Pereskia spp. have superior ovaries (Bravo-Hollis 1978;
Gibson and Nobel 1986). Cacti are among the few plants
in which the exterior of an inferior ovary is sunken into a
modified stem, termed the receptacle (Booke 1980). The
ovary is formed by the fusion of several carpels and consists
of a single internal chamber, the locule, where a relatively
high number of ovules occur in a parietal location along
the ventral wall of the ovary (Fig. 5.2; Booke 1980). The
number of ovules per flower is 150 to 400 for Opuntia ficusindica (Rosas and Pimienta 1986; Weiss et al. 1993), 388
(hermaphrodites) to 406 (females) for O. robusta (del
Castillo 1986a), over 1,000 for Stenocereus queretaroensis
(Pimienta-Barrios and Nobel 1995), and 7,200 for
Hylocereus undatus (Nerd and Mizrahi 1997).Fgiure52.neahrere:
A unique type of ovule is found in the Cactaceae. Due
to unilateral growth, it first becomes anatropous and, as
the curvature continues, the micropyle again points upward in the fully formed ovule (Bhojwani and Bhatnagar
1979). Embryological observations for Opuntia and
Stenocereus spp. (Rosas and Pimienta 1986; Ortega, 1993;
76
Pimienta-Barrios and del Castillo
Figure 5.1. Opuntia flower at the initial stages of opening, when only
the basal part of the stigma is covered with pollen grains released
before opening from anthers in contact with the basal part of the
stigmatic surface.
García-Aguilar and Pimienta-Barrios 1996) reveal that
most of the embryological characters are primitive, according to Grayum’s (1991) criteria. The embryological
characters are: (1) the inferior position of the ovary, (2) four
microsporangia per anther, (3) isobilateral type of microspore tetrads, (4) bitegmic and crassinucelated ovules,
(5) the functional megaspore in the chalazal position, (6)
embryo of the polygon type, (7) the high number of ovules
per ovary, and (8) glandular tapetal tissue (Grayum 1991;
Ortega 1993).
The style can be short and thick, as for Opuntia spp.,
or long and thin, as for Heliocereus spp. (Arreola-Nava
1997). The stigma is at the apex of the style (Fig. 5.2),
where pollen is deposited, and shows a variable number of
lobes — from 3, as for Mammillaria, to 24 for Hylocereus
(Arreola-Nava 1997). The stigma of most cactus flowers
shows characteristics suitable for insect landing, because it
is over the stamens (Grant and Grant 1979b; Ross 1981; del
Castillo and González-Espinosa 1988). Both the large stigmatic surface and the stickiness of the receptive surface improve the chances of catching pollen grains. The nectary
usually lies at the base of the thickened style, forming a
chamber at the base of the receptacle, where substantial
nectar accumulates. Nectar can be rich in sucrose, as for
Pachycereus pringlei (Fleming et al. 1994), and is usually secreted at the beginning of anthesis (del Castillo and
González-Espinosa 1988; Mandujano et al. 1996). In the
Peruvian genus Matucana, the nectar chamber is closed by
a protective device that probably prevents dilution of the
nectar (Bregman 1996).
The numerous stamens of a cactus flower are spirally
arranged and have thigmotropic sensitivity; i.e., they move
9
8
7
6
5
4
2
3
1
Figure 5.2. Longitudinal section of a Mammillaria flower: (1) peduncular zone; (2) receptacular tissue; (3) locular cavity; (4) ovules in a
parietal position; (5) receptacular tube; (6) stamen; (7) style; (8) stigmatic lobule; and (9) perianth segment. Modified from Bravo-Hollis
(1978).
when touched (Rosa and Pimienta 1986). The thigmotropic movements can facilitate pollen collection by insects during floral visits (Grant and Hurd 1979) and also
promote self-pollination (Rosas and Pimienta 1986). The
number of pollen grains produced per flower varies from
160,000 for O. rastrera (Mandujano et al. 1996) to 330,000
for O. robusta male plants (del Castillo 1986a). The number of ovules per flower for opuntias varies from 150 to 400
(Rosas and Pimienta 1986; Nerd and Mizrahi 1997), so the
number of pollen grains per ovule varies from 400 to 800.
The ratio is low compared with that for anemophilous
(wind pollinated) flowers producing 500,000 to 3,000,000
pollen grains per ovule, and from 5,000 to 100,000 pollen
grains per ovule for entomophilous (insect pollinated)
plants (Linskens 1983). Similarly low pollen grains-to-ovule
ratios occur for five species of Venezuelan columnar cacti:
Pilosocereus moritzians, P. lanuginosus, Stenocereus griseus,
Subpilocereus horispinus, and S. repandus — 300 to 1,050
(Nassar et al. 1997). Low investment of energy in the pro-
duction of male gametophytes may be a strategy to save energy in stressful environments.
Flower Types, Pollinators, and Pollination
Studies on cactus pollination span more than a century
(Toumey 1895; Mandujano et al. 1996), although many
genera of cacti have not been studied at all. The pollination
vectors for cacti are animals that exhibit specificity but not
exclusivity. Based on the main pollinators attracted, cactus
flowers can be roughly classified as bee flowers, moth
flowers, hummingbird flowers, and bat flowers (Porsch
1938, 1939; Grant and Grant 1979c).
The typical bee flower is bowl- or cup-shaped (Fig. 5.1)
and has many perianth segments, a brightly color perianth,
diurnal periodicity, numerous stamens, a single style, and
a lobed stigma (Grant and Hurd 1979; del Castillo and
González-Espinosa 1988; del Castillo 1994, 1999). The perianth is usually yellow, but may be pinkish, orange, magenta, red, or violet. The color may turn darker after the
flowers are pollinated, as for some opuntias. Red or other
colored stripes are present in some species of Ferocactus,
Mammillaria, and Stenocactus. Ultraviolet floral patterns
may increase pollinator visits and efficiency, as observed for
Echinocereus spp. (Leuck and Miller 1982).
Although butterflies, diptera, beetles, and hummingbirds are also flower visitors, bees are the most frequent and
the most likely pollinators. Nearly 20 genera of native bees
as well as the common honey bee, Apis mellifera, are flower
visitors, but not all of them are equally efficient for pollination. Various factors apparently determine the effectiveness for bee pollination of cacti: (1) flying activity, (2)
flower size, (3) constancy, and (4) the adherence of pollen
grains to the body. Large bees, such as bumblebees
(Bombus spp., 11–19 mm in body length), may be important pollinators because, although they are generalists, they
perform long-distance flights that favor outcrossing between distant individuals. Medium-sized bees, such as
Diadasia spp. (10–14 mm in body length), are the major
pollinators of several species whose flowers are 50 to 70
mm in perianth diameter, such as many Opuntia spp. (Fig.
5.1; Grant and Hurd 1979; del Castillo and GonzálezEspinosa 1988) and Echinocereus spp. (Grant and Grant
1979a; Leuck and Miller 1982). They have good pollen adherence to their bodies, are very active flying among the
flowers, and are the most common floral visitors for these
species. They usually land on the stigma, where the pollen
is deposited, and then submerge into mass of stamens to
visit the nectary and collect nectar.
Bee pollinators select whether or not to visit a cactus
flower based on floral size. For bee-pollinated species with
Reproductive Biology
77
a small floral diameter (40–45 mm), such as Ferocactus
histrix and Diadasia spp., medium-sized bees are less-frequent visitors. Instead, smaller bees (e.g., Ashmeadiella
spp., 5–7 mm in body length) are the major pollinators
(del Castillo 1994). These bees also are less common
flower visitors for opuntias and, because of their small size,
when they do enter flowers, they may do so without touching the stigma (del Castillo and González-Espinosa 1988).
Cacti with small, bowl-shaped flowers, such as Epithelantha spp., are probably mostly autogamous and depend little on pollinators to set seed (Grant and Grant 1979c).
Small bees visit the small, bowl-shaped flowers of Mammillaria spp. Pollinators are needed even for certain selfcompatible species to set seed, such as O. lindheimeri
(Grant et al. 1979). Usually nectar is the main reward for
bees. Some species, however, do not produce nectar, e.g.,
O. lindheimeri (Grant et al. 1979). Because this species coexists with nectar-producing opuntias with the same floral
design, this may be considered an example of Batesian
mimicry for cacti (Roy and Widmer 1999). It may also be
an adaptation for moisture conservation (Grant and Hurd
1979).
The number of cacti that produce bee flowers suggests
that this flower type is the most successful, or at least most
common where cacti evolved. Perhaps its major disadvantage lies in the promiscuity of the pollinators, which may
be a problem in areas rich in cactus diversity, where pollination among different species or genera is common
(Leuck and Miller 1982; Grant and Hurd 1979; GarcíaSánchez 1984; del Castillo 1994). Promiscuity enhances hybridization and probably promotes evolutionary changes
of the breeding system or the pollinator syndromes. A filter
that maintains the individuality is the specificity of the pollination mechanisms (Linskens 1983).
Typical hummingbird flowers are red, diurnal, tubular,
and zygomorphic. The stamens usually rise well above the
tepals. Interestingly, this floral syndrome is more common
in epiphytic cacti from humid habitats, such as cloud
forests, than other cactus habitats. Many epiphytic species
of Schlumbergera (McMillan and Horobin 1995), Disocactus, and Nopalxochia also have this floral syndrome.
Opuntia spp. seems to have an interesting evolutionary
transition from a bee-pollinated syndrome to the hummingbird syndrome. Some species have color variations
that diverge from the typical yellow flower of many beepollinated cacti to red, while preserving the same floral
shape (Arias-Moreno and Arreola-Nava 1995). The flowers
of O. stenopetala are red, have a closed perianth, are nearly tubular, and are attractive to hummingbirds. Nopalea,
which is closely related to Opuntia, has a typical hum-
78
Pimienta-Barrios and del Castillo
mingbird flower and is commonly visited and probably
pollinated by these birds. In addition, the South American
genus Tacinga, which is intermediate between Nopalea and
Opuntia, has flowers with erect and non-sensitive stamens
(typical of Nopalea) and narrow, green, recurved petals
(Britton and Rose 1937).
Pollination by bats occurs for columnar cacti, such as
Carnegia gigantea (Alcorn et al. 1961), Neobuxbaumia spp.
(Valiente-Banuet et al. 1997), Pachycereus pringlei (Fleming
et al. 1994), Pilosocereus spp. (Zappi 1994), Stenocereus
pruinosus (Valiente-Banuet et al. 1997), and S. stellatus
(Ramírez-Mireles 1999). Bat-pollinated flowers are usually large, robust, nocturnal, white or cream, and odoriferous, and produce abundant nectar. The flowers of columnar cacti attract bees and hummingbirds during the
daytime and moths during nighttime (Valiente-Banuet et
al. 1997). Leptonycteris spp. are common pollinators of cacti
with bat-attracting flowers, and they may consume the
pollen (Alcorn et al. 1961). Symbiosis with bats has at least
three advantages for cacti: (1) lower promiscuity compared
to bee flowers, (2) long distance flying (Fleming et al.
1994), and (3) seed dispersal by the bats (Fleming et al.
1994; Valiente-Banuet et al. 1997).
Moths commonly visit large, nocturnal, white, discshaped flowers, which are partially zygomorphic (most of
the stamens lie on one side of the flower) and have a large
nectarial chamber. Many genera of epiphytic cacti, such as
Hylocereus (Ramírez-Mireles 1999), Echinopsis, Epiphylum,
and Selenicereus (Rowley 1980), bear these flowers. Flowers
of Hylocereus open at dusk but may remain open until the
next morning, when they are visited by bees and bumblebees, which appear to be the major pollinators (OrtízHernández 1999; Ramírez-Mireles 1999). Nectar is a major
reward for moths. In their attempt to reach the nectary,
moths visiting flowers of Hylocereus leave many of their
wing scales on the style, darkly staining it and thus providing an easy means for detecting moth visits (Y. OrtízHernández, personal communication). Some cylindropuntias have nocturnal disc-shaped flowers, instead of the
typical diurnal bowl-shaped flowers (Grant and Hurd
1979).
Whereas bees, bats, hummingbirds, and moths are attracted by cactus flowers and pollinate them, the role of
other flower visitors, such as beetles, ants, and birds other
than hummingbirds, is not well established. Beetles commonly visit flowers of many species of cacti, such as those
with bowl-shaped flowers (e.g., Echinocereus, Ferocactus,
Echinocactus, Mammillaria, and Opuntia; Grant and
Connell 1979; Grant and Grant 1979a; Grant and Hurd
1979; García-Sánchez 1984; del Castillo and González-
Espinosa 1988; del Castillo 1994). However, they are not
good pollinators, as few pollen grains adhere to their bodies. Also, some beetles stay in a single flower, chewing floral
parts. In fact, beetles in the genera Camptodes, Carpophilus,
and Trichochrous invade the flowers in large groups and
perform few flights among them (Grant and Connell 1979;
Grant and Hurd 1979; García-Sánchez 1984; del Castillo
and González-Espinosa 1988; del Castillo 1994). These beetles may self-pollinate the flowers directly or by stimulating thigmotrophic movements of the stamens, favoring the
deposition of self-pollen on the stigma. In most cases, however, beetles may be nectar and pollen thieves. For Pilosocereus, a columnar cactus pollinated by bats, small beetles
lay eggs in the flowers. The emerging larvae perforate the
ovary and the stem (Zappi 1994).
Carnegiea gigantea in the Sonoran Desert can be pollinated by western white-winged doves, although they are
not the main pollinator (Alcorn et al. 1961). In the
Galápagos Islands, finches are common visitors of Opuntia
echios and O. helleri, from which they remove the stigma
to gain access to pollen and the nectar. In getting a shortterm benefit, they potentially suffer in the long term
through a diminished supply of seeds, which they also eat
during the dry season (Grant and Grant 1981). Ants also
visit cactus flowers. They may be attracted by the
extrafloral nectaries, a modified spine that secretes nectar
in various genera, such as Coryphantha, Ferocactus, and
Opuntia. Nectar production usually coincides with
flowering or fruiting. Ants do not pollinate the flowers, but
they may help protect the plant from potential herbivores.
For Ferocactus histrix, territorial and entomophagous ants
(genera Dorymyrmex and Iridomyrmex) consume the nectar of extrafloral nectaries. They may even build their nests
on top of the plants (del Castillo 1988a). For O. acanthocarpa, ants attracted by the extrafloral nectaries may increase fruit set and decrease fruit abortion by reducing the
herbivore activity of coreid (Hemiptera: Coreidae) bugs
(Pickett and Clark, 1979).
Outcrossing Mechanisms
Outcrossing is common among cacti. Of the 55 taxa studied by Ross (1981), seeds are produced upon self-pollination in only 11 taxa and by cross-pollination in the other 44
taxa. Cacti have several adaptations favoring outcrossing:
self-incompatibility, dichogamy, herkogamy, and unisexuality. Incompatibility is a genetic barrier in the progamic
phase that may take place between pollen and stigma or
during the development of pollen tubes in the style. The
latter occurs for some Opuntia spp. (Bullock 1985; Rosas
1984; Negrón-Ortiz 1998), for varieties of Hylocereus spp.
(Ramírez-Mireles 1999), and for Schlumbergera (McMillan
and Horobin 1995). The inhibition of the pollen tube in
the style, after it has penetrated the stigma, is a characteristic of the gametophytic system of incompatibility (Lewis
1979; de Nettancourt 1997). But cacti do not share all of
the characteristics typical of this incompatibility mechanism. For instance, the pollen in cacti is tri-nucleate
(Benson 1979), whereas pollen in the typical gametophytic system is bi-nucleate (de Nettancourt 1997).
Other examples of obligate outcrossing cacti are
Carnegia gigantea (Alcorn et al. 1961), Ferocactus histrix,
Nopalea auberi (R. del Castillo, personal observations),
Neobuxbaumia spp. (Valiente-Banuet et al. 1997), Opuntia
helleri, and O. echios (Grant and Grant 1981). Selfincompatibility also occurs for Astrophytum, Pediocactus,
Stenocereus griseus, S. repandus, S. horrispinus, and Thelocactus (Nassar et al. 1997) and in clones of Cereus peruvianus, Hylocereus costaricensis, and H. polyrhizus (Weiss et
al. 1994a,b, 1995). For O. leucotricha, both selfing and
interspecific hybrid crosses lead to low rates of fruit and
seed set relative to outcrossing (Trujillo and GonzálezEspinosa 1991).
In several hermaphroditic species of cacti, male and
female organs mature at different times inside the flower.
This phenomenon, called dichogamy, may reduce self-fertilization. For instance, in Hylocereus spp. cultivars, the
stigma becomes receptive about 3 hours after the anthers
dehisce (Ramírez-Mireles 1999), and pollen germinability
is highest at anthesis. For F. histrix, pollen is released 1 to
2 days after anthesis. At this stage, the stigma lobes are
closed, and the pollinators usually land directly on the stamens to reach the nectary. In this way, pollen can be easily collected. After 2 to 3 days of opening, the stigma lobes
expand and are used by pollinators as a landing platform,
and pollen from others flowers can be deposited (del
Castillo 1994). Therefore, selfing within the same flower is
avoided but the transfer of pollen from one flower to another in the same plant (geitonogamy) is not, as flowering
is sequential.
Whereas dichogamy implies a temporal separation in
the maturation of male and female flower parts, herkogamy is the spatial separation of anthers and stigma. The degree to which the stigmatic area is in contact with the anthers influences the probability of selfing. Grains of self
pollen can occur on the lower portions of the stigma,
which is in contact with the anthers, whereas outcross
pollen is deposited on the upper surface of the stigma, as
seen in a cultivar of O. ficus-indica (Fig. 5.1; Rosas and
Pimienta 1986). Moreover, the fraction of the stigmatic surface in contact with the anthers is positively correlated with
Reproductive Biology
79
the rate of autogamy for Opuntia (Trujillo and GonzálezEspinosa 1991). For Hylocereus undatus, the distance between the stigma and anthers is large, thus decreasing the
probability of autogamy (Y. Ortíz-Hernández, personal
communication). This distance—and thus the probability
of outcrossing — can change during the flowering season
(Grant et al. 1979). The distance between the style and the
stigma of Nopalea spp. changes during flower maturation.
During opening when the pollen is released, the distance
is less, increasing later when the stigma becomes receptive.
While most cacti are hermaphroditic, interesting exceptions occur for Echinocereus, Mammillaria, Neobuxbaumia, Opuntia, Pachycereus, and Selenicereus (del Castillo
1986a; Fleming et al. 1994; Valiente-Banuet et al. 1997).
Atrophied organs of the non-functional sex and close hermaphroditic relatives of unisexual individuals suggest that
unisexuality is a derived condition for cacti, as for other
flowering plants. Unisexuality appears to have evolved several times independently. Dioecious populations (a population containing both male and female individuals) have
been detected in Echinocereus coccineus, Opuntia robusta,
and O. stenopetala (del Castillo and González-Espinosa
1988; Fleming et al. 1998). Trioecy (male, female, and hermaphroditic individuals in a single population) is reported for O. robusta and Pachycereus pringlei (del Castillo and
González-Espinosa 1988; Fleming et al. 1998), gynodioecy
(female and hermaphroditic individuals) for P. pringlei
(Fleming et al. 1998), and androdioecy (male and hermaphroditic individuals in the same population) for
Neobuxbaumia mezcalaensis (Valiente-Banuet et al. 1997).
Polyploidy and Reproduction
Cacti have a great range of polyploidy (Pinkava et al. 1977,
1985; Cota and Philbrick 1994). The significance of polyploidy, however, has not been related to the biology of the
plants, particularly the mode of reproduction (Ross 1981).
Changes in polyploidy can modify the breeding system of
plants by at least two mechanisms: (1) by modifying the
magnitude of inbreeding depression, and (2) by breaking
down incompatibility mechanisms. Some species of cacti
that have low rates of inbreeding depression are tetraploids
—e.g., O. robusta (Sosa and Acosta 1966) and Pachycereus
pringlei (Fleming et al. 1994). Ross (1981) reviewed 55 taxa
of cacti and noted that polyploidy is correlated with selffertility. In other families, chromosome doubling breaks
down incompatibility mechanisms (Lewis 1979). Differences in inbreeding depression among diploids and polyploids probably have large influences on the evolution of
polyploidy and breeding systems of cacti. Triploidy and
tetraploidy most likely originated from fertilization in-
80
Pimienta-Barrios and del Castillo
volving gametes that had not undergone a reduction in
their chromosome number (Lewis 1980), and fertilization
of unreduced gametes accounts for several intraspecific
polyploid Opuntia hybrids (Pinkava et al. 1985). A comparison of the ploidy level with the mode of reproduction
in the Cactaceae suggests that polyploidy is more likely to
become established in self-fertile or apomictic (producing
seeds in the absence of fertilization) taxa (Ross 1981).
Flower Fertilization
The processes that occur from the arrival of pollen grain
deposition onto the stigma to when the sperm reach the
egg cell have received little attention (Rójas-Aréchiga and
Vázquez-Yanes 2000). For the progamic phase of Opuntia
ficus-indica, anther dehiscence occurs just before or at the
time of flower opening, so pollen grains can start germination on the stigmas a few hours before the flowers open
(Rosas 1984). Each stigma receives many pollen grains, but
only a few germinate (about 30%; Weiss et al. 1993).
Consequently, relatively few pollen tubes grow in the
upper part of the style—from 300 to 350 per flower (Table
5.1). Pollen grain germination and pollen tube growth
occur relatively rapidly, with over 20 pollen tubes occurring
at the base of the style within 24 hours after flower opening, increasing to 65 tubes by 48 hours after flower opening. By the time the style wilts (72 hours after pollination),
the number of pollen tubes at the base of the style exceeds
100 (Table 5.1).Taben51l.eahrere:
Ovule fertilization is porogamous for both Opuntia
and Stenocereus, because the pollen tubes penetrate the
ovule through the micropyle before reaching the embryo
sac (Rosas and Pimienta 1986; Ortega 1993). The first
ovules with signs of fertilization occur 2 days after flower
opening for Opuntia. At this time, the percentage of fecund ovules is low (2%), reaching 46% 2 days later.
Because of the high number of ovules per flower (over
250), the ovule fertilization continues until 10 days after
pollination. Ovule viability is high, because the percentage
of ovules that are fertilized and transformed in seeds is high
(over 80%; Rosas and Pimienta 1986).
Flower and Fruit Development
Early ontogeny of cactus flowers, including the initiation of
floral organs, is similar to that in many other species. In the
Northern Hemisphere, flower bud differentiation for most
cacti begins at the end of winter and the beginning of
spring; anthesis begins in the spring followed by the development of fruit, which mature during the summer, e.g., for
Opuntia spp. (Bravo-Hollis 1978; Trujillo 1982; del Castillo
and González-Espinosa 1988; Pimienta-Barrios 1990;
TA B L E 5 . 1
Position and frequency of pollen tubes at different times after natural pollination for flowers of
Opuntia ficus-indica
Number of pollen tubes at different positions along style
Hours after
pollination
Stigma–¼
¼–½
½–¾
¾–base of style
24
48
72
344 ± 49
355 ± 41
325 ± 26
192 ± 10
220 ± 25
225 ± 26
63 ± 29
126 ± 35
151 ± 20
21 ± 4
65 ± 9
102 ± 31
Data are means ± SE (n = 10 styles). Adapted from Rosas and Pimienta (1986).
Mandujano et al. 1996). For Echinocactus, Ferocactus, Myrtillocactus, Pachycereus, and Stenocereus, flower differentiation and fruit development start earlier, and fruit ripening
occurs at the end of the spring (Trujillo 1982; Gibson and
Nobel 1986; del Castillo 1988a; Pimienta-Barrios 1999).
Another common reproductive feature of cacti is that
flower development is asynchronous. Namely, flowers in
early stages of differentiation, flowers at anthesis, and
young developing fruits can occur simultaneously (Fig. 5.3;
Trujillo 1982; del Castillo 1988a; Pimienta-Barrios 1990;
Lomelí and Pimienta 1993). The asynchronous patterns of
flower and fruit production may be especially advantageous
during adverse environmental conditions (Pimienta-Barrios
and Nobel 1995). Also, some cacti may have a second
blooming, as for Stenocereus griseus (Piña 1977).Fgiure53n.eahrere:
The time between flower bud differentiation and
flower opening is relatively short for cacti—30 to 35 days
for Cereus peruvianus and Hylocereus spp. (Nerd and
Mizrahi 1997), 40 to 50 days for Opuntia spp. (PimientaBarrios 1990), 45 to 60 days for Stenocereus megalanthus
(Nerd and Mizrahi, 1997), and 40 days for S. queretaroensis (Pimienta-Barrios and Nobel 1998). This behavior is
similar to that for other tropical and subtropical fruit
crops, such as orange, avocado, and mango (PimientaBarrios 1990), but contrasts with that for temperate fruit
crops (e.g., apple, peach, pear) whose flower initiation usually occurs 1 year before flowering (Faust 1989).
Flower bud initiation for Opuntia spp. is inhibited by
shade and gibberellic acid (GA). Both shade and GA are
effective when applied before the vegetative meristem in
the areoles begins its transformation to the reproductive
condition, as indicated by the flattening of its dome.
Areoles in which GA inhibit flower bud differentiation
show a marked increase in the number of spines (PimientaBarrios 1990), perhaps reflecting a reversion to the juvenile
phase, as occurs for Hedera helix (Rogler and Hackett 1975).
Low temperatures in the winter influence flower bud
burst for some cultivated cacti, indicating that chilling is
involved (Nerd and Mizrahi 1997). Horticulturists argue
that bud burst occurs at the end of the winter in cultivated cacti. In the highlands of Ayacucho, Peru, flower bud
burst occurs throughout the year for Opuntia ficus-indica,
suggesting that other physiological factors can be involved
in the control of flower induction and bud break. Thus,
flower bud initiation and control may not rely on a single
factor, such as chilling.
The time of flowering in certain species of cacti is little influenced by water availability. For instance, many
species of cacti in Mexico and the southwestern United
States start flowering (e.g., Echinocactus platyacanthus,
Trujillo 1982; Echinocereus spp., Powell et al. 1991; Neobuxbaumia spp., Valiente-Banuet et al. 1997) or have their
flowering peaks (e.g., Ferocactus histrix, del Castillo 1988a;
Opuntia spp., Rodríguez-Zapata 1981, del Castillo and
González-Espinosa 1988; Pachycereus pringlei, Fleming et al.
1994) in March or April. These months are among the driest and near the end of the longest drought period of the
year. Although reproductive development occurs during
the dry season in arid regions of both the Northern and
Southern Hemispheres, relatively few fruits abscise and a
high percentage of flowers become fruits. In contrast, the
reproductive growth of many other fruit crops is highly
sensitive to drought. For instance, the percentage of
flowers that become fruit can be 95% for O. ficus-indica
and 28% for Stenocereus queretaroenis but is often under
10% for many fruit crops, such as apple, avocado, mango,
and orange (Stephenson 1981; Pimienta-Barrios 1990;
Pimienta-Barrios et al. 1995). This may in part reflect the
succulence of the cactus stems, which can store appreciable amounts of water that can be available to reproductive
structures during drought (Gibson and Nobel 1986).
Reproductive growth for O. ficus-indica is highly re-
Reproductive Biology
81
Bud
Flowers
Fruits
number of flowers
and fruits per plant
30
20
10
0
j
f
m
a
m
j
month (1994)
Figure 5.3. Reproductive development for Stenocereus queretaroensis
under natural conditions, indicating the emerging flower buds (0),
open flowers (∆), and nearly ripe fruits ( ). Adapted from PimientaBarrios and Nobel (1995).
sponsive to management. Both flower number and fruit
number dramatically increase in response to both mineral
and organic fertilizers, water, and pruning (PimientaBarrios 1990). In contrast, irrigation does not affect stem extension, reproductive demography, fruit quality, or seed size
for S. queretaroensis (Pimienta-Barrios and Nobel 1995).
The time from flower opening to fruit maturation is
relatively short, particularly for columnar cacti. Fertilized
flowers give rise to mature fruits 40 to 50 days after pollination for Pilosocereus lanuginosus, Stenocereus griseus, and
Subpilocereus repandus (Petit 1995). For S. queretaroensis,
fruits attain over 90% of their final length in about 40 days,
and the overall development period from anthesis to maturity is about 90 days (Pimienta-Barrios and Nobel 1995).
Fruits of the vinelike climbing cacti Hylocereus costaricencis,
H. polyrhizus, and H. undatus have a short growth period of
about 50 days, whereas fruits of Selenicereus megalanthus require 90 to 150 days (Weiss et al. 1994b, 1995).
Seed Germination
Both ovule fertilization and seed germination are critical
stages in plant development. Despite the importance of
seed germination in the reproduction of cacti, the pioneer
studies on seed germination are relatively recent. Alcorn
and Kurtz (1959) and McDonough (1964) revealed the importance of light. But Zimmer (1969) found that some
species do not require light for germination and that the
cactus species that require light for germination have a
greater response when they are exposed to red light. Pilcher
(1970) indicated the presence of dormancy for Opuntia
82
Pimienta-Barrios and del Castillo
seeds, an observation later confirmed for a large number of
Opuntia species (Bregman and Bouman 1983; Pérez 1993).
Although most cactus seeds germinate within a week, germination for subfamily Opuntioideae often takes a few
months (Bregman and Bouman 1983; Pérez 1993; Table 5.2).
Seed dormancy, characterized by the perseverance of the
dormant condition even when the seeds are exposed to optimal environmental conditions for germination, has survival value for cacti growing in arid environments. Both innate and enforced dormancy are common for most cacti
(Rójas-Aréchiga and Vázquez-Yanes 2000).Tabe25l.neahrer:
A low level of light (PPF < 20 µmol m-2 s-1) is required
for the germination of most cactus seeds. Red light stimulates germination for soaked seeds of Stenocereus griseus,
but the effect of red light is reversed by far-red light, suggesting the participation of phytochrome in the seed germination process (Martínez-Holguín 1983). The application of gibberellic acid also increases seed germination for
S. griseus (Moreno et al. 1992; López-Gómez and SánchezRomero 1989). Thus, phytochrome may exert its control of
seed germination through the synthesis of gibberellic acid.
The seeds of S. griseus also maintain their viability after 12
months of storage, reaching germination percentages of 90
to 100% four days after sowing (López-Gómez and
Sánchez-Romero, 1989). Maiti et al. (1994) suggest that a
high germination percentage is associated with a thin testa
and with the presence of starch granules. Seed germination
for Stenocereus queretaroensis is relatively high even though
its seeds are small (2.6 mg); the seeds have a thin testa and
a relatively large amount of lipid (Pimienta et al. 1995).
Seeds of many wild species that respond to light are also
rich in lipids. Because of their small size and light requirements, such seeds should be near the soil surface for successful germination.
Besides light, the germination of cactus seeds requires
wet conditions and responds to temperature (RójasAréchiga and Vázquez-Yanes 2000). Water uptake stimulates cracks in the testa (outer seed coat) caused by the
growing embryo (Bregman and Bouman 1983) and leaches possible germination inhibitors (Mondragón-Jacobo
and Pimienta-Barrios 1995). The presence of soluble inhibitory substances in the testa of cactus seeds maintains
the dormant state until environmental conditions are
suitable for development (Rójas-Aréchiga and VázquezYanes 2000). The fractional germination of seeds of some
cacti increases for the first 3 years after harvest, or after the
passage through the digestive tracts of rabbits. Seeds that
pass through the digestive tract of cattle exhibit average
germination percentages that are 50% higher than seeds re-
TA B L E 5 . 2
Percentage of seed germination of cultivated and wild opuntias species at different times after
fruit ripening
Time (months) after fruit ripening
Common name (species)
Cultivated
Burrona (Opuntia spp.)
Chapeada (O. ficus-indica)
Cristalina (O. spp.)
Naranjona (O. ficus-indica)
Wild
Cardona (O. streptacantha)
Negrita (O. streptacantha)
Tapona (O. robusta)
2
4
6
8
12
14
0
0
0
0
0
0
0
0
0
0
0
0
0
0
3
5
3
28
85
42
23
80
90
50
0
0
0
0
0
0
3
13
8
23
55
3
40
67
80
30
100
32
Adapted from Mondragón-Jacobo and Pimienta-Barrios (1995).
moved from ripe fruits (Potter et al. 1984). For cacti, the
optimal temperature for seed germination ranges from 17
to 34°C, with a mean of 25°C (Nobel 1988). Alternating
temperatures give higher germination yields than constant
temperatures (Rójas-Aréchiga and Vázquez-Yanes, 2000).
For Opuntia species, optimum constant temperatures vary
from 25 to 35°C (Mondragón-Jacobo and Pimienta-Barrios
1995). For Astrophytum myriostigma, germination rates are
highest (80–98%) at temperatures from 20 to 25°C, and
decrease at higher temperatures under conditions of diffuse
light (Moreno et al. 1995).
For species of Opuntia and Stenocereus, among others,
seed dormancy is apparently related to the time of fruit
ripening. For instance, fruit ripening for S. queretaroensis
and Myrtillocactus geometrizans occurs at the end of the
spring, in contrast with Opuntia, for which fruit ripening
for most species occurs from the middle of summer to the
beginning of the autumn. The occurrence of fruit ripening
at the end of the spring for S. queretaroensis and M. geometrizans may be optimal, because seed maturation occurs
just before the start of the summer, which favors seed germination and establishment because of favorable soil
moisture and shading by surrounding natural vegetation in
their habitats (Fig. 5.4; Nobel 1988; Pimienta-Barrios and
Nobel 1998). In contrast, seed development for Opuntia
spp. occurs near the middle or the end of the rainy season,
so its seed maturation and dispersal coincide with the beginning of the dry season and the reduction of air temperatures in Mexico.Fgiure54.neahrere:
To avoid drought stress and frost damage, seeds of
Opuntia spp. have dormancy periods of 7 to 8 months;
seed germination is initiated at the start of the next rainy
season (Pérez 1993). However, seed germination for other
cacti indicates that dormancy is not related to the time of
fruit ripening. Seeds of some barrel cacti (e.g. Ferocactus
flavovirens, F. histrix, F. latispinus, and F. robustus) and certain columnar cacti (e.g., Cephalocereus chrysacanthus,
Neobuxbaumia, and Pachycereus hollianus) do not show
dormancy, as high percentages of their seeds germinate
without scarification or washing, once the fruit is mature
(Bregman and Bouman 1983; del Castillo 1986b; RójasAréchiga et al. 1998). However, the seeds of Echinocereus
polyacanthus do not germinate in the winter, at the time of
fruit ripening, suggesting that low temperatures or short
days can induce dormancy for this species (Trujillo 1982).
Given the short and unpredictable rain periods occurring
in most arid and semiarid habitats, the absence of dormancy for cactus seeds may be considered a selective advantage, as a quick onset of germination would enable
seedling establishment despite an erratic environment.
Apomixis
In addition to sexual reproduction, two types of asexual reproduction are present in cacti: vegetative apomixis
and agamospermy. In vegetative apomixis, a new plant is
derived after the rooting of plant fragments or ramets detached from a parent plant, as occurs for Myrtillocactus
geometrizans. Among the Cactaceae, Opuntia spp. are
probably the best examples of reproductive versatility,
using a wide array of sexual and asexual methods. Indeed,
Reproductive Biology
83
Drought
Rainy
Drought
Rainy
Opuntia
Fruit
dehiscence
Reproductive
development
Seed
Stenocereus
Fruit
dehiscence
Reproductive
development
Seed
J
F
M
A
M
J
J
A
S
O
N
D
J
F
M
A
M
J
J
A
S
O
N
D
Figure 5.4. The time of reproductive development, fruit dehiscence, and seed germination with respect to drought and rainfall periods for
Opuntia and Stenocereus spp. Adapted from Pimienta-Barrios (1999).
propagation through vegetative parts is common for them
in both natural and cultivated populations in arid regions
(Grant and Grant 1971; Nava et al. 1981; Pimienta-Barrios
1990; Mandujano et al. 1996, 1998; Negrón-Ortiz 1998).
Vegetative apomixis is particularly important, because the
propagules carry reserves of water and energy, allowing
them to withstand prolonged drought. Indeed, certain
species of this genus, especially cylindropuntias, reproduce
exclusively or nearly exclusively asexually.
One disadvantage of asexual reproduction can be its
low dispersion. In many species of Opuntia, however, vegetative apomixis can be highly successful, in part due to its
high dispersability. The cladodes of platyopuntias and the
joints of cylindropuntias can be readily detached and adhere by means of the spines to the skin of cattle, wild life,
and other animals that touch the plants. This favors the invasion of opuntias in overgrazed areas (Anthony 1954).
Humans also play an important role in spreading opuntias
vegetatively. In semiarid areas of central Mexico, shepherds
and cattle ranchers commonly cut off the cladodes of
opuntias for animal feeding or to have easy access when
walking. Some of the cladodes, or even their fragments,
84
Pimienta-Barrios and del Castillo
may root and give rise to new individuals. Indeed,
nopaleras, a vegetation type in central Mexico dominated
by Opuntia spp., may be anthropogenic (GonzálezEspinosa 1999). Vegetative apomixis can cause opuntias to
become noxious weeds. The epibiotic outbreak of Opuntia
stricta in Australia after its introduction in 1839 is the best
example. In 1920 this species occupied 24 × 106 hectares
and was colonizing about 400,000 hectares per year. The
moth Cactoblastis cactorum was brought from Argentina to
control the outbreak (Harper 1977).
Some asexual species of Opuntia provide the best examples of high dispersal rates among cacti. Opuntia fragilis, for instance, is a low plant, 5 to 10 cm tall, with an
enormous geographic range from Chihuahua and Coahuila in Mexico (Bravo-Hollis 1978) up to Saskatchewan,
Alberta, and British Columbia in Canada, including 16
states in the United States (Mitch 1970). Its altitudinal
range is from sea level up to 2,400 m in a great variety of
vegetation types. Flowering and fruit production are rare,
and fruits are usually sterile (Benson 1982; Mitch 1970).
Bison probably helped to disperse it (Mitch 1970). Another
example is O. polyacantha, which has sterile fruits, high
phenotypic diversity, and a wide geographic distribution
from Chihuahua and Coahuila, Mexico, to Canada
(Benson 1982; Mitch 1970).
In certain species of Opuntia, sexual reproduction occurs only sporadically. For instance, despite its high seed
production, most of the population regeneration of O. rastrera is through asexual reproduction (Mandujano et al.
1996), although in grassy areas of its range, O. rastrera reproduces mainly sexually (Mandujano et al. 1998). Germination rates for various Opuntia spp. are usually low, at
least soon after the seed is released (Trujillo and GonzálezEspinosa 1991). Germination rates may increase steadily
with aging (Mandujano et al. 1997). However, predation
rates in natural habitats may also be high, particularly by
rodents (González-Espinosa and Quintana-Ascencio 1986).
Thus, mutations favoring asexual reproduction may be
common. Indeed, asexual clones can be derived from sexual species. Opuntia ficus-indica produces normal seeds,
but sterile clones occur in which mutation back to sexual
reproduction is possible (Weiss et al. 1993).
Whereas vegetative apomixis is widespread among opuntias and may contribute to their large geographic range,
it is less important in the rest of the Cactaceae. For vegetative apomixis to be an efficient means of dispersal, the
plants must have small detachable units, as happens for
small opuntias, such as O. fragilis. In many opuntias with
large cladodes, vegetative apomixis may not be an efficient
way of reproduction, because sooner or later the limited
dispersal would cause the saturation of suitable available
habitat (cf. the strawberry-coral model; Williams 1975). In
certain species of Opuntia, however, humans can circumvent this problem by promoting vegetative reproduction
artificially, as may have happened for the Mexican
nopaleras. Other cactus genera, such as Hylocereus, can be
propagated vegetatively (Ortiz-Hernández 1999). However,
reproduction by seed is the most common reproductive
method for most cacti.
For certain plants, normal seed is set but sexual fusion
does not occur. Compared to vegetative apomixis, agamospermy has the advantage of seminifery, because the seed
is a vehicle of dispersal that confers resistance to environmental extremes, such as through dormancy (HeslopHarrison 1983). As for vegetative apomixis, agamospermy
is also a common asexual reproduction method for species
of Opuntia (Ganong 1898; Archibald 1939; Tiagi 1954;
Flores and Engleman 1973; García-Aguilar and PimientaBarrios 1996; Négron-Ortiz 1998), as well as Mammillaria
(Ross 1981). Polyembryonic seeds are common in most of
the wild and cultivated Opuntia spp. growing in semiarid
central Mexico (Trujillo and González-Espinosa 1982;
Figure 5.5. Multiple proembryos in the embryos sac of the polyembryonic Opuntia streptacantha, showing a laminar zygote proembryo
(z) and globular adventitious proembryos (g). Scale bar = 50 µm.
Pérez 1993). For example, seeds of many wild opuntias have
two and, on rare occasions, three embryos (Trujillo and
González-Espinosa 1982), and the percentages of polyembryonic seeds varies from 0.5 to 20% (Pérez 1993). The production of both sexual and asexual seeds may be considered a facultative apomixis, exchanging genetic material by
occasionally producing sexual material and achieving a balance between stability and flexibility that permits adaptation to stressful environments.
Mammillaria prolifera is apomictic by adventitious embryos. However, some primarily outcrossing (allogamous)
taxa, e.g., Mammillaria tenuis and M. zeilmanniana, are
partially apomictic by adventitious embryos after endosperm formation. Self-sterile polyploids of M. compressa, M.
parkinsona, and Gymnocalccium brunchii have extensive
vegetative branching. Opuntia spp., which have a high frequency of vegetative propagation, adventitious embryos,
and self-fertility, have extensive polyploidy (Ross 1981).
Embryos from apomictic seeds generally originate
from nuclear tissue, and nuclear embryogenesis commonly occurs without pollination (Tisserat et al. 1979). However, a comparative study of embryo-sac development in a
Reproductive Biology
85
monoembryonic/polyembryonic Opuntia species to assess
the cytological origin of both sexual and agamospermic
embryos reveals that, in the polyembrionic species, multiple embryos are differentiated in the central cell of the embryo sac at the mycropilar side; however, pollen tubes do
not penetrate the mycropile, indicating that fertilization
does not occur (García-Aguilar and Pimienta-Barrios
1986). The embryos located at the periphery of the embryo
sac are globular and without a suspensor, differing from a
well-developed laminar embryo with a well-defined suspensor located at the mycropilar side of the embryo sac
(Fig. 5.5). Because of their position in the embryo sac and
their morphology, the laminar embryo with its suspensor
apparently differentiates from the egg cell, and the globular embryos without suspensors derive from the nuclear
cells and further invade the embryo sac (Bhojwani and
Bhatnagar 1979; Tisserat et al. 1979). The lack of evidence
of meiotic chromosome reduction during megasporogenesis and fertilization in the polyembryonic species suggests
that the laminar embryo, with a well-defined suspensor,
develops by diplospory-parthenogenesis (gametophytic
apomixis), and the globular embryos without suspensors
develop by adventitious embryony (Heslop-Harrison
1983).Fgiure5n.eahrere:
Conclusions
The reproductive systems of cacti include such striking features as combining of two or more reproductive methods
and switching between reproductive modes during the lifetime of an individual. Asexual reproduction can fix favorable combinations of genes, which were produced previously by sexual reproduction. Selfing can be an economic
way of sexual reproduction and also functions as a genetic barrier, preventing or reducing hybridization and permitting the production of seed when external pollination
is unreliable. Outcrossing is undoubtedly a genetic method
favoring the wide diversity observed for cacti. But all of
these forms of reproduction have genetic costs (e.g., segregational load with outcrossing, inbreeding depression
with selfing, and probably mutational load with asexual reproduction) and ecophysiological costs to the plant (e.g.,
in a particular outcrossing, attracting pollinators and resource demands). In some cases, the partial or complete
suppression of one of these reproductive systems has been
successful, as for species with unisexual individuals or the
sterile or partially sterile opuntias.
The reproductive versatility is extremely widespread in
members of the genus Opuntia, and it can play an important role in the ecological strategy of adaptation to aridity.
Not surprisingly, Opuntia is the most widely distributed
86
Pimienta-Barrios and del Castillo
genus in the Cactaceae. Members of this genus have selfpollinating as well as cross-pollinating flowers. Both sexual reproduction and asexual reproduction by vegetative
parts and seeds occur. These versatile reproductive systems
are often controlled by environmental factors, suggesting
that the genetic systems may exhibit phenotypic plasticity.
Although asexual reproduction can be very successful, in
some circumstances sexual reproduction, or the mixed
mating system (most likely the ancestral form) with its
combination of outcrossing and inbreeding, predominates. The evolutionary importance of sexual reproduction
is reflected by its existence, persistence, ubiquity, and presumably lower energy investment, despite the great potential among cacti for asexual reproduction.
Literature Cited
Alcorn, S. M., and E. B. Kurtz. 1959. Some factors
affecting the germination of seeds of the saguaro cactus (Carnegia gigantea). American Journal of Botany 46:
526–529.
Alcorn, S. M., McGegor, S. E., and S. E. Olin. 1961.
Pollination of saguaro cactus by doves, nectar feeding
bats, and honey bees. Science 133: 1594–1595.
Anthony, M. 1954. Ecology of the Opuntiae in the Big
Bend region of Texas. Ecology 35: 334–347.
Archibald, A. E. E. 1939. The development of the ovule
and seed of joined cactus (Opuntia aurantiaca
Lindley). South African Journal of Science 36: 195–211.
Arias-Moreno, S. 1997. Distribución general. In Suculentas
Mexicanas Cactáceas. Editorial CVS Publicaciones,
Mexico City. Pp. 17–25.
Arias-Montes, S., and H. Arreola-Nava. 1995. Consideraciones sobre Opuntia decumbens Salm-Dick. Cactáceas
y Suculentas Mexicanas 40: 86–92.
Arreola-Nava, H. 1997. Formas de vida y características
morfológicas. In Suculentas Mexicanas Cactáceas.
Editorial CVS Publicaciones, Mexico City. Pp. 27–35.
Benson, L. 1979. Plant Classification. D. C. Heath, Lexington, Massachusetts.
Benson, L. 1982. The Cacti of the United States and Canada.
Stanford University Press, Stanford, California.
Berry, J. A., I. P. Ting, and E. Zeiger. 1983. The biology of
desert plants: Opportunities and needs for basic research. Conference Report, October 24–25, University
of California Philip L. Boyd Deep Canyon Desert
Research. American Society of Plant Physiologist,
Rockville, Maryland.
Bhojwani, S. S., and P. Bhatnagar. 1979. The Embryology of
Angiosperms. Vikas Publishing House, New Delhi.
Booke, N. H. 1980. Developmental morphology and
anatomy in Cactaceae. BioScience 30: 605–610.
Bravo-Hollis, H. 1978. Las Cactáceas de México, Vol. 1.
Universidad Nacional Autónoma de México, Mexico
City.
del Castillo, R. F., and M. González-Espinosa. 1988. Una
interpretación evolutiva del polimorfismo sexual de
Opuntia robusta (Cactaceae). Agrociencia 71: 185–196.
de Nettancourt, D. 1997. Incompatibility in angiosperms.
Sexual Plant Reproduction 10: 185–199.
Faust, M. 1989. Physiology of Temperate Zone Fruit Trees.
Wiley, New York.
Bregman, R. 1996. The genus Matucana: Biology and systematics of a fascinating Peruvian cactus. A. Balkema,
Rotterdam, The Netherlands.
Fischer, R. A., and N. C. Turner. 1978. Plant production in
the arid and semiarid zones. Annual Review of Plant
Physiology 29: 277–317.
Bregman, R., and F. Bouman. 1983. Seed germination in
Cactaceae. Botanical Journal of the Linnean Society 86:
357–374.
Fleming, T. H., S. Maurice, S. L. Buchmann, and M. D.
Tuttle. 1994. Reproductive biology and relative fitness
in a trioecious cactus, Pachycereus pringlei (Cactaceae).
American Journal of Botany 81: 858–867.
Britton, N., and J. N. Rose, 1937. The Cactaceae. Dover,
New York.
Bullock, S. H. 1985. Breeding systems in the flora of a tropical deciduous forest in Mexico. Biotropica 17: 287–
304.
Buxbaum, F. 1950. Morphology of Cacti, Section I. Roots and
Stems (Edwin B. Kurtz, ed.). Abbey Garden Press,
Pasadena, California.
Cota, J. H., and C. T. Philbrick. 1994. Chromosome
number variation and polyploidy in the genus
Echinocerus (Cactaceae). American Journal of Botany 81:
1054–1062.
del Castillo, R. F. 1986a. La selección natural de los sistemas de cruzamiento en Opuntia robusta. Master’s
Thesis, Colegio de Postgraduados, Montecillo,
Mexico.
del Castillo, R. F. 1986b. Semillas, germinación y establecimiento de Ferocactus histrix. Cactáceas y Suculentas
Mexicanas 31: 5–11.
del Castillo, R. F. 1988a. Nota sobre los nectarios extraflorales en cactáceas. Cactáceas y Suculentas Mexicanas
33: 99–100.
del Castillo, R. F. 1988b. Fenología y remoción de semillas
en Ferocactus histrix. Cactáceas y Suculentas Mexicanas
33: 5–14.
del Castillo, R. F. 1994. Polinización y otros aspectos de la
biología floral de Ferocactus histrix. Cactáceas y
Suculentas Mexicanas 39: 36–43.
del Castillo, R. F. 1999. Exploración preliminar sobre los
sistemas de cruzamiento en Opuntia. In El Nopal.
Memoria, VIII Congreso Nacional y VI Internacional
sobre el Conocimiento y Aprovechamiento del Nopal,
Universidad Autónoma de San Luis Potosí, San Luis
Potosí, Mexico. Pp. 360–389.
Fleming, T. H., S. Maurice, and J. L. Hamrick. 1998.
Geographic variation on the breeding system and evolutionary stability in Pachycereus pringlei (Cactaceae).
Evolutionary Ecology 12: 279–289.
Flores, V. M. E., and E. M. Engleman. 1973. Apuntes
sobre anatomía y morfología de las semillas de cactáceas. I. Desarrollo y estructura. Revista de Biología
Tropical 24: 199–207.
Ganong, W. F. 1898. Upon polyembriony and its morphology in Opuntia vulgaris. Botanical Gazette 25: 221–228.
García-Aguilar, M., and E. Pimienta-Barrios. 1996. Cytological evidences of agamospermy in Opuntia (Cactaceae). Haseltonia 4: 39–42.
García-Sánchez, R. 1984. Patrones de polinización y
fenología floral en poblaciones de Opuntia spp. en San
Luis Potosí y Zacatecas, México. Bachelor’s Thesis,
Universidad Nacional Autónoma de México, Los
Reyes Iztacala.
Gibson, A. C., and P. S. Nobel. 1986. The Cactus Primer.
Harvard University Press. Cambridge, Massachusetts.
González-Espinosa, M., and P. F. Quintana-Ascencio.
1986. Seed predation and dispersal in a dominant
desert plant: Opuntia, ants, birds and mammals. In
Frugivores and Seed Dispersal (A. Estrada and T. H.
Fleming, eds.). Dr. D. W. Junk, Dordrecht, The
Netherlands. Pp. 273–284.
González-Espinosa, M. 1999. Interacciones entre fenología,
elementos bióticos y disturbio por pastoreo en las
nopaleras del centro de México. In El Nopal. Memoria,
VIII Congreso Nacional y VI Internacional sobre el
Conocimiento y Aprovechamiento del Nopal. Universidad
Autónoma de San Luis Potosí, San Luis Potosí, Mexico.
Pp. 342–359.
Reproductive Biology
87
Grant, B. R., and P. R. Grant. 1981. Exploitation of Opuntia
cactus by birds on the Galápagos. Oecologia 49: 179–
187.
Lomelí, M. E., and E. Pimienta. 1993. Demografía reproductiva del pitayo (Stenocereus queretaroensis (Web.)
Buxbaum). Cactáceas y Suculentas Mexicanas 38: 13–20.
Grant, V. K., and W. A. Connell. 1979. The association between Carpophilus beetles and cactus flowers. Plant
Systematics and Evolution 133: 99–102.
López-Gómez, R., and P. Sánchez-Romero. 1989.
Germinación de dos variedades de pitayo Stenocereus
griseus (Haworth) Buxbaum. Cactáceas y Suculentas
Mexicanas 34: 35–41.
Grant, V. K., and K. A. Grant. 1971. Dynamics of clonal
microspecies in Cholla cactus. Evolution 25: 144–155.
Grant, V. K., and K. A Grant. 1979a. Pollination of
Echinocereus fasciculatus and Ferocactus wislizenii. Plant
Systematics and Evolution 132: 85–90.
Grant, V. K., and K. A. Grant. 1979b. Pollination of
Opuntia basiliaris and O. littoralis. Plant Systematics
and Evolution 132: 321–325.
Grant, V. K., and K. A. Grant. 1979c. The pollination
spectrum in the Southwestern American cactus flora.
Plant Systematics and Evolution 133: 29–37.
Grant, V. K., and P. D. Hurd. 1979. Pollination of the
Southwestern Opuntias. Plant Systematics and Evolution 133: 15–28.
Grant, V. K., K. A. Grant, and P. D. Hurd. 1979.
Pollination of Opuntia lindheimeri and related species.
Plant Systematics and Evolution 132: 313–320.
Grayum, M. H. 1991. Systematic embryology of the
Araceae. Botanical Review 57: 167–203.
Harper, J. L. 1977. Population Biology of Plants. Academic
Press, New York.
Heslop-Harrison, J. 1983. The reproductive versatility of
flowering plants: An overview. In Strategies of Plant
Reproduction (W. J. Meudt, ed.). Beltsville Symposia in
Agricultural Reseach. Allanheld, Osmun/Granada,
London. Pp. 3–18.
Leuck E. E., and J. M. Miller. 1982. Pollination biology
and chemotaxonomy of the Echinocereus viridiflorus
complex (Cactaceae). American Journal of Botany 69:
1669–1672.
Lewis, D. 1979. Sexual Incompatibility in Plants. Edward
Arnold, London.
Lewis, W. H. 1980. Polyploidy in Angiosperms. Dicotyledons. In Polyploidy, Biological Relevance (D. H. Lewis,
ed.). Plenum Press, New York. Pp. 110–120.
Linskens, F. H. 1983. Pollination processes: Understanding
fertilization and limits to hybridization. In Strategies of
Plant Reproduction (G. W. J. Meudt, ed.). Beltsville
Symposium in Agricultural Research. Allanheld,
Osmund/Granada, London. Pp. 35–49.
88
Pimienta-Barrios and del Castillo
Maiti, R. K., J. L. Hernández-Piñero, and M. ValdezMarroquín. 1994. Seed ultrastructure and germination
of some species of Cactaceae. Phyton 55: 97–105.
Mandujano, M. C., C. Montaña, I. Mendez, and L.
Eguiarte. 1996. Reproductive ecology and inbreeding
depression in Opuntia rastrera (Cactaceae) in the
Chihuahuan Desert. Why are sexually derived recruitments so rare? American Journal of Botany 83: 63–70.
Mandujano, M. C., J. Golubov, and C. Montaña. 1997.
Dormancy and endozoochorus dispersal of Opuntia
rastrera seeds in the southern Chihuhuan Desert.
Journal of Arid Environments 36: 259–66.
Mandujano, M. C., C. Montaña, I. Mendez, and J.
Golubov. 1998. The relative contribution of sexual reproduction and clonal propagation in Opuntia rastrera
from two habitats in the Chihuahuan Desert. Journal
of Ecology 86: 911–921.
McDonough, W. 1964. Germination responses of Carnegia
gigantea and Lemairocereus thurberi. Ecology 45: 155–
159.
McMillan, A. J. S., and J. B Horobin. 1995. Christmas
Cacti: The Genus Schlumbergera and Its Hybrids. Royal
Botanical Gardens, Kew, England.
Mitch, L. W. 1970. Wyoming native cacti. Cactus and
Succulent Journal (U.S.) 42: 155–159.
Mondragón-Jacobo, C., and E. Pimienta-Barrios. 1995.
Propagation. In Agro-Ecology, Cultivation, and Uses of
Cactus Pear (G. Barbera, P. Inglese, and E. PimientaBarrios, eds.). FAO Plant Production and Protection
Paper 132. FAO, Rome. Pp. 64–70.
Moreno, N. P., López, J. J., and L. Arce. 1992. Aspectos
sobre las semillas y su germinacion de Echinomastus
mariposensis Hester. Cactáceas y Suculentas Mexicanas
37: 21–27.
Nassar, M. J., N. Ramírez, and O. Linares. 1997.
Comparative pollination biology of Venenzuelan
columnar cacti and the role of nectar-feeding bats in
their sexual reproduction. American Journal of Botany
84: 918–927.
Nava, R. C., J. J. López G., and J. Gasto, C. 1981.
Dinámica poblacional del ecosistema natural de
Opuntia streptacantha Lemaire. Monografía Técnica
Científica, Serie Recursos Naturales, Universidad
Autónoma Agraria Antonio Narro 7: 219–271.
characteristics of pitayo (Stenocereus queretaroensis)
and their relationships with soluble sugars and irrigation. Journal of the American Society for Horticultural
Science 120: 1082–1086.
Negrón-Ortiz, V. 1998. Reproductive biology of a rare cactus, Opuntia spinosissima (Cactaceae), in the Florida
Keys: Why is seed set very low? Sexual Plant
Reproduction 11: 208–221.
Pimienta-Barrios, E., and P. S. Nobel. 1998. Vegetative, reproductive, and physiological adaptations to aridity of
pitayo (Stenocereus queretaroensis, Cactaceae). Economic
Botany 52: 401–411.
Nerd, A., and Y. Mizrahi. 1997. Reproductive biology of
cactus fruit crops. Horticultural Reviews 16: 321–346.
Pimienta-Barrios, E., C. Robles-Murguía, and A.
Domínguez de la Torre. 1995. Estrategias fisiológicas y
reproductivas de adaptación del pitayo a la aridez.
Ciencia 46: 339–349.
Nobel, P. S. 1995. Environmental biology. In Agro-Ecology,
Cultivation, and Uses of Cactus Pear (G. Barbera, P.
Inglese, and E. Pimienta-Barrios, eds.). FAO Plant
Production and Protection Paper 132. FAO, Rome. Pp.
36–48.
Nobel, P. S. 1988. Environmental Biology of Agaves and
Cacti. Cambridge University Press, New York.
Ortega, C. M. M. 1993. Diferenciación de los gametofitos
masculino y femenino en pitayo (Stenocereus queretaoensis (Weber) Buxbaum) y su relación con aspectos
evolutivos. Bachelor’s Thesis, Universidad de Guadalajara, Guadalajara, Mexico.
Ortíz-Hernández, Y. D. 1999. Pitahaya. Un nuevo Cultivo
para México. Editorial Limusa, Mexico City.
Osborn, M. M., P. G. Kevan, and M. A. Lanen. 1988.
Pollination biology of Opuntia polyacantha (Cactaceae)
in southern Colorado. Plant Systematics and Evolution
159: 85–94.
Piña, L. I. 1977. Pitayas y otras cactáceas afines del Estado
de Oaxaca. Cactáceas y Suculentas Mexicanas 22: 3–14.
Pinkava, D. J., L. A. McGill, and T. Reeves. 1977.
Chromosome numbers in some cacti of western North
America—III, with nomenclature changes. Bulletin of
the Torrey Botanical Club 104: 105–110.
Pinkava, J. D., M. A. Baker, B. D. Parfitt, and M. W.
Mohlenbrock. 1985. Chromosome numbers in some
cacti of western North American—V, with nomenclature changes. Systematic Botany 10: 471–483.
Porsch, O. 1938. Das Bestäubunsleben der Kakteenblüte, I.
Cactaceae. Jahrbuch Deutsche Kakteen-Gesellschaft 1938:
1–80.
Porsch, O. 1939. Das Bestäubunsleben der Kakteenblüte,
II. Cactaceae. Jahrbuch Deutsche Kakteen-Gesellschaft
1939: 81–142.
Pérez, R. C. 1993. Viabilidad de semillas y poliembrionía
en morfoespecies silvestres y cultivadas de Opuntia spp.
Bachelor’s Thesis, Universidad de Guadalajara, Guadalajara, Mexico.
Potter, I. R., J. L. Petterson, and D. N. Ueckert. 1984.
Germination response of Opuntia spp. to temperature,
scarification, and other seed treatments. Weed Science
32: 106–110.
Petit, S. 1995. The pollinators of two species of columnar
cacti on Curaçao, Netherlands Antilles. Biotropica 27:
538–541.
Powell, J. R., A. D. Zimmerman, and R. A. Hilsenbeck.
1991. Experimental documentation of natural hybridization in Cactaceae: Origin of Lloyd’s Hedgehog
cactus, Echinocereus lloydii. Plant Systematics and
Evolution 178: 107–122.
Pickett, C. H., and W. D. Clark. 1979. The function of
extrafloral nectaries in Opuntia acanthocarpa (Cactaceae). American Journal of Botany 66: 618–625.
Pilcher, B. L. 1970. Germination of seeds of four species of
Opuntia. Cactus and Succulent Journal (U.S.) 42: 281–
282.
Pimienta-Barrios, E. 1990. El Nopal Tunero. Universidad de
Guadalajara, Zapopan, Mexico.
Pimienta-Barrios, E. 1999. El Pitayo en Jalisco y Especies
Afines en Mexico. Universidad de Guadalajara, Zapopan,
Mexico.
Pimienta-Barrios, E., and P. S. Nobel. 1995. Reproductive
Primack, R. B. 1982. Longevity of individual flowers.
Abstracts, Annual Meeting of the Botanical Society of
America. University of California, Davis. P. 41.
Ramírez, N., and P. E. Berry. 1995. Producción y costo de
frutos y semillas entre modos de polinización en 232 especies de plantas tropicales. Revista de Biología Tropical
43: 51–159.
Ramírez-Mireles, F. J. 1999. Caracterización y compatibilidad en pitahaya. Bachelor’s Thesis, Universidad
Autónoma Chapingo, Chapingo, Mexico.
Reproductive Biology
89
Rodríguez-Zapata, O. 1981. Fenología reproductiva y
aporte de frutos y semillas en dos nopaleras del Altiplano Potosino-Zacatecano. Bachelor’s Thesis, Universidad Autónoma de Nuevo León, Monterrey,
Mexico.
Toumey, J. W. 1895. Vegetal dissemination of the genus
Opuntia. Botanical Gazette 20: 356–361.
Rogler, L. E., and W. P. Hackett. 1975. Nutrient diversion:
An hypothesis to explain the chemical control of
flowering. HortScience 12: 220–222.
Trujillo, A. S. 1982. Estudio sobre algunos aspectos ecológicos de Echinocactus platyacanthus Lk & O. en el estado de San Luis Potosí. Bachelor’s Thesis, Universidad
Nacional Autónoma de México, Iztacala.
Rójas-Aréchiga, M., C. Vázquez-Yanes, and A. OrozcoSegovia. 1998. Seed response to temperature of two life
forms of Mexican cacti species: An ecophysiological interpretation. Plant Ecology 135: 207–214.
Trujillo, A. S., and M. González-Espinosa. 1991. Hibridación, aislamiento reproductivo y formas de reproducción en Opuntia spp. Agrociencia, Serie Recursos
Naturales Renovables 1: 39–58.
Rójas-Aréchiga, M., and C. Vázquez-Yanes. 2000. Cactus
seed germination: A review. Journal of Arid Environments 44: 85–104.
Valiente-Banuet, A., A. Rójas-Martínez, A. M. del Coro,
and P. Davila. 1997. Pollination biology of two columnar cacti (Neobuxbaumia mezcalaensis and Neobuxbaumia macrocephala) in the Tehuacán Valley, Central
Mexico. American Journal of Botany 84: 452–455.
Rosas, C. M. P. 1984. Polinización y fase progámica en
Opuntia spp. Bachelor’s Thesis, Universidad Michoacana de San Nicolás de Hidalgo, Morelia, Mexico.
Rosas, C. M. P., and E. Pimienta. 1986. Polinización y fase
progámica en nopal (Opuntia ficus-indica (L.) Miller).
Tunero Fitotecnia 8: 164–176.
Ross, R. 1981. Chromosome counts, cytology, and reproduction in the Cactaceae. American Journal of Botany
68: 463–70.
Rowley, G. 1980. Pollination syndromes and cactus taxonomy. Cactus and Succulent Journal (Great Britain) 42:
95–98.
Weiss, J., A. Nerd, and Y. Mizrahi. 1993. Vegetative
parthenocarpy in the cactus pear Opuntia ficus-indica
(L.) Mill. Annals of Botany 72: 521–526.
Weiss, J., A. Nerd, and Y. Mizrahi. 1994a. Flowering and
pollination requirements in climbing cacti with fruit
crop potential. HortScience 29: 1487–1492.
Weiss, J., A. Nerd, and Y, Mizrahi. 1994b. Flowering and
pollination requirements in Cereus peruvianus cultivated in Israel. Journal of Plant Science 42: 149–158.
Roy, B. A., and R. E. Widmer. 1999. Floral mimicry: A fascinating yet poorly understood phenomenon. Trends in
Plant Science 4: 325–330.
Weiss, J., L. Scheinvar, and Y. Mizrahi. 1995. Selenicereus
megalanthus (the yellow pitaya), a climbing cactus from
Colombia and Peru. Cactus and Succulent Journal
(U.S.) 67: 280–283.
Sosa, R., and C. A. Acosta. 1966. Poliploidia en Opuntia.
Agrociencia 1: 100–106.
Williams, G. C. 1975. Sex and Evolution. Princeton
University Press, Princeton, New Jersey.
Stephenson, A. G. 1981. Flower and fruit abortion:
Proximate and ultimate functions. Annual Review of
Ecology and Systematics 12: 253–279.
Zappi, D.C. 1994. Pilosocereus (Cactaceae): The Genus in
Brazil. Royal Botanical Gardens, Kew, England.
Tiagi, D. Y. 1954. Studies in the floral morphology of
Opuntia dilleni Haworth. Botaniska Notiser 4: 343–356.
Tisserat, B., E. B. Esan, and T. Murashige. 1979. Somatic
90
embryogenesis in angiosperms. Horticultural Reviews 1:
1–78.
Pimienta-Barrios and del Castillo
Zimmer, K. 1969. Über die Keimung von Kakteensamen
III. Die Bedeutung des Lichtes. Kakteen und andere
Sukkulenten 20: 114–147.
CHAPTER
›6‹
P O P U L AT I O N A N D
C O M M U N I T Y E C O LO G Y
Alfonso Valiente-Banuet and Héctor Godínez-Alvarez
Introduction
Population Ecology
Seed Germination
Seedling Establishment and Growth
Reproduction and Breeding Systems
Seed Dispersal
Population Dynamics
Community Ecology
Conclusions and Future Prospects
Literature Cited
Introduction
Cacti are a typical component of vegetation in tropical and
subtropical America (Gibson and Nobel 1986; Barthlott
and Hunt 1993; Valiente-Banuet et al. 1995a). Although
some species reach high latitudes in British Columbia and
Alberta in Canada and in the Patagonian region of
Argentina, species diversity increases considerably toward
the tropics, Mexico being the most important center with
about 850 species and 54 genera (Bravo-Hollis 1978; Arias
1993). The tribes Opuntieae, Cacteae, and Echinocereeae,
including species with laterally flattened stems in the
genus Opuntia, globose or barrel cacti in the genera
Coryphantha, Echinocereus, Mammillaria, and many monospecific genera such as Ariocarpus, Astrophytum, Aztekium,
and Lophophora, are widely distributed in the Chihuahuan
Desert of Mexico with almost 400 endemic species (Arias
1993). In the Sonoran Desert, seven species of columnar
cacti (tribe Pachycereeae) with genera such as Carnegiea,
Pachycereus, and Stenocereus dominate the arid landscapes
in northwestern Mexico, forming the sarcocaulescent
desert of Shreve (1951), which also has many species of
cylindropuntias and barrel cacti (Turner et al. 1995). In
south-central Mexico in the Balsas River Basin and the
Tehuacán Valley, the tribe Pachycereeae with its arborescent cacti is highly diversified; this tribe contains 45 of the
70 species of local columnar cacti in Mexico, constituting
the principal structural and floristic elements of columnarcactus forests (Valiente-Banuet et al. 1995a, 1996). Other
tribes, such as Hylocereeae, are basically restricted to
humid tropical regions in southeastern Mexico and
Central America and are represented mostly by epyphitic
cacti (Arias 1993). The genus Pereskia is an important component of the tropical deciduous forest of southern
Oaxaca, Mexico (Bravo-Hollis 1978).
In these geographical areas, cacti show a considerable
diversity of life form, and the different types of vegetation
acquire their names depending on their physiognomic
91
and/or structural dominance (Miranda and Hernández
1963; Rzedowski 1978; Osorio et al. 1996). Spectacular examples of these plant associations include the Cardonales,
dominated by columnar cacti called “cardones” since the
Spanish conquest times, belonging to the genera Stenocereus, Pachycereus, Cephalocereus, and Mitrocereus; the
Nopaleras, a name derived from the term “nopal,” which is
the Nahuatl name for the flat-stemmed species of Opuntia,
consist of dense stands of several species, and “Tetecheras”
and “Giganteras,” which are dominated by different species
of Neobuxbaumia. The key aspects in the population and
community ecology of these plants are considered in this
chapter to determine how abiotic and biotic factors interact to influence the distribution and abundance of a particular species, leading to particular survivorship, fecundity, growth patterns, and groups of species, with an
emphasis on the maintenance of biodiversity.
Population Ecology
Cacti have lifespans of decades to hundreds of years
(Steenbergh and Lowe 1977). Along their life cycle, the
different stages, such as the seed and seedling, juvenile, mature, and senile plants, are exposed to different mortality
factors related to high radiation levels, water stress, and biotic interactions such as predation and competition. Early
stages of the life cycle are the most important for maintaining viable populations in the field (Steenbergh and
Lowe 1969, 1977; Valiente-Banuet and Ezcurra 1991;
Godínez-Alvarez et al. 1999). Therefore, the successful production of seeds, their dispersal and germination, seedling
establishment, and the survivorship of seedlings and juveniles are essential for the maintenance of cactus populations under natural conditions. These stages constitute a
link between the reproductive adults and the new individuals (Howe and Smallwood 1982); thus, the main goal of
this section is to analyze their relative effect on the population dynamics of cacti.
Seed Germination
Most studies analyzing seed germination of cactus species
have been conducted under controlled conditions in the
laboratory (Chapter 5) and few have considered seed germination under field conditions. Dubrovsky (1996, 1998)
reported that the seeds of the Sonoran Desert cacti Carnegiea gigantea, Ferocactus peninsulae, Pachycereus pectenaboriginum, Stenocereus gummosus, and S. thurberi, subjected to hydration-dehydration cycles of different lengths,
germinated faster and accumulated higher biomass compared to untreated seeds. Thus, seeds apparently retain
during dehydration the physiological changes promoted by
92
Valiente-Banuet and Godínez-Alvarez
seed hydration. This “seed hydration memory” may facilitate seed germination, increasing the survival of cacti in
the field (Dubrovsky 1996).Tabe6ln1.eahrere:
Light stimulates seed germination for only some
species (Table 6.1). Rojas-Aréchiga et al. (1997) divided
cacti into two groups: (1) globose or barrel cacti such as
Mammillaria, Echinocactus, and Ferocactus that require
light for the seed germination (i.e., positively photoblastic),
and (2) columnar cacti in the genera Neobuxbaumia,
Cephalocereus, Pachycereus, and others for which seed germination is not affected by light. This correlation between
light and lifeform in cacti may result from the environmental and maternal effects on seeds during their development (Rojas-Aréchiga et al. 1997). Light requirements
are also affected by temperature regimes, washing of the
seeds, and gibberellic acid (Rojas-Aréchiga and VázquezYanes 2000). Ecologically, lack of light can inhibit germination when the seeds are deep in the soil, and light can
stimulate germination when soil moisture is available
(Kigel 1995). Germination occurs for a wide range of temperatures, 10 to 40°C (Nobel 1988; Rojas-Aréchiga and
Vázquez-Yanes 2000). The optimal temperature for germination is 20 to 30°C. In addition to these responses, extreme temperatures and aging of seeds decrease germination (Nobel 1988; Rojas-Aréchiga and Vázquez-Yanes
2000).
Seedling Establishment and Growth
The establishment phase and early seedling growth in
deserts occurs under unpredictable conditions of precipitation and in soils with high temperatures and low water
content. Under these circumstances, cacti are often established beneath the canopy of perennial “nurse plants”
(Table 6.2), which modify the environment beneath their
canopies. Nurse plant phenomenon have been addressed
by determining the spatial relationships between cacti and
perennial plants through nearest-neighbor analysis. Both
positive and negative effects for cacti are derived from the
association with nurse plants, as evidenced by means of a
cost-benefit analysis (Franco and Nobel 1989). Among the
positive effects of nurse plants on seeds and seedlings is
protection against direct solar radiation, leading to a decrease in extreme soil temperatures and hence increasing
the soil moisture available for seed germination and early
seedling survival (Turner et al. 1966; Steenbergh and Lowe
1969, 1977; Franco and Nobel 1989; Valiente-Banuet and
Ezcurra 1991; Nolasco et al. 1997). Surface temperatures in
open spaces between nurse plants can be up to 65°C,
whereas in the shaded microsites under nurse plants soil
surface temperatures are 10 to 20°C lower (Turner et al.
TA B L E 6 . 1
Effect of light and temperature on seed germination of some cactus species
Temperature 2
Species
Cephalocereus chrysacanthus
Coryphantha pallida
Echinocactus platyacanthus
Ferocactus flavovirens
F. recurvus
F. robustus
Myrtillocactus geometrizans
Neobuxbaumia tetetzo
Opuntia puberula
Pachycereus hollianus
P. pringlei
Stenocereus thurberi
Light1
Minimum
Optimum
Maximum
0
10
40
+
+
+
+
15
15
15
15
0
10
17
10
30
17–25
25
15–25
25
30
17–25
15–30
20–25
15–20
0
0
0
40
40
40
35
40
40
1. Indifferent to light (0) or positively photoblastic (+). References: Nolasco et al. (1996,
1997) and Rojas-Aréchiga et al. (1997).
2. Temperatures (°C) at which minimum, optimum, and maximum germination are
observed. References: Godínez-Alvarez and Valiente-Banuet (1998), Nolasco et al. (1996),
and Rojas-Aréchiga et al. (1998).
1966; Franco and Nobel 1989; Valiente-Banuet et al. 1991a;
Arriaga et al. 1993; Suzán et al. 1996). Nurse plants can also
provide protection against wintertime low temperatures,
decreasing the susceptibility of seedlings to frost injury
(Brum 1973; Steenbergh and Lowe 1969, 1977) and enhancing the establishment of C. gigantea and S. thurberi in
marginal populations where seedlings are exposed to extreme low temperatures during the winter (Brum 1973;
Steenbergh and Lowe 1969, 1977; Parker 1987).Tabe6l2.neahrere:
Some nurse plants in arid ecosystems increase the nitrogen content of soils under their canopies compared to
open spaces. This increment in soil nitrogen, which can
reflect interactions with soil microorganisms, creates “islands of fertility” in which the growth rates of seedlings
and their survival probability increase (García-Moya and
McKell 1970; Franco and Nobel 1989; Valiente-Banuet and
Ezcurra 1991; Godínez-Alvarez and Valiente-Banuet 1998).
Nevertheless, the empirical evidence on the increase of soil
nitrogen content is controversial, as some studies support
this hypothesis (García-Moya and McKell 1970; Franco
and Nobel 1989) whereas others do not (Valiente-Banuet et
al. 1991a,b; Arriaga et al. 1993).
Protection against seed and seedling predators is another benefit associated with nurse plants (Steenbergh and
Lowe 1969, 1977; McAuliffe 1984a; Hutto et al. 1986;
Valiente-Banuet and Ezcurra 1991). Birds, mammals, and
insects are among the main cactus predators; however, the
protection provided by nurse plants against predators and
therefore on the survival of seeds and seedlings depends on
the foraging patterns of each predator (Hutto et al. 1986).
In this respect, Valiente-Banuet and Ezcurra (1991) found
that the seeds of Neobuxbaumia tetetzo are similarly predated by harvester ants and ground-foraging birds under
nurse plants and in open spaces. On the other hand,
McAuliffe (1984a) found that herbivores consume less
Mammillaria microcarpa and Echinocereus engelmannii
growing under the canopies of the tree-like cactus Opuntia
fulgida, because spiny nurse plants inhibit the foraging beneath their canopies.
Among negative effects, nurse plants reduce the photosynthetic photon flux (PPF) and increase the competition for limited supplies of water (Franco and Nobel 1989).
For instance, the nurse plants of young C. gigantea and
Ferocactus acanthodes reduce the total daily available PPF
by about 70%. This reduction reduces net CO2 uptake,
decreasing the growth rate compared to seedlings in open
spaces. Moreover, the competition between seedlings and
nurse plants for water can also affect seedling growth.
However, such negative effects can be offset by increases in
soil fertility under the canopies of nurse plants (Franco and
Nobel 1989). Additionally, Altesor et al. (1992) indicate that
during the first 10 to 20 weeks after germination, seedlings
Population and Community Ecology
93
TA B L E 6 . 2
Species of cacti and their nurse plants
Species
Nurse plant(s)
Carnegiea gigantea
Ambrosia deltoidea
Cercidium microphyllum
Encelia farinosa
Larrea tridentata
Olneya tesota
Prosopis juliflora
Steenbergh and Lowe (1969);
Hutto et al. (1986); Franco and
Nobel (1989)
Cephalocereus hoppenstedtii
Caesalpinia melanadenia
Valiente-Banuet et al. (1991a)
Coryphantha pallida
Castela tortuosa
Eupatorium odoratum
Echinocereus engelmannii
Opuntia fulgida
McAuliffe (1984a)
Echinomastus erectocentrus
Celtis pallida
Lycium andersonii
L. berlandieri
Olneya tesota
Zizyphus obtusifolia
Suzán et al. (1996)
Ferocactus acanthodes
Ambrosia dumosa
Ephedra aspera
Pleuraphis rigida
Franco and Nobel (1989)
F. peninsulae
Haematoxylon brasiletto
Arriaga et al. (1993)
Lophocereus schottii
Celtis pallida
Lycium andersonii
L. berlandieri
Olneya tesota
Zizyphus obtusifolia
Suzán et al. (1996)
Mammillaria casoi
Caesalpinia melanadenia
Valiente-Banuet et al. (1991a)
M. collina
Castela tortuosa
Eupatorium odoratum
of Ferocactus recurvus, Neobuxbaumia tetetzo, and Opuntia
pilifera exhibit a C3 metabolism, which can increase the
seedling growth rate during this phase and partially compensate for the lower PPF levels.
Because suitable conditions for seed germination and
seedling establishment for many cactus species occur beneath the canopies of nurse plants, cacti can have a
clumped distribution pattern in association with trees and
shrubs. Moreover, plants may interact competitively with
individuals of their own species (i.e., intraspecific competition) and/or with their nurse plants (i.e., interspecific
competition). Carnegiea gigantea competes intraspecifically, affecting the rate of water uptake, the relative amount
94
Reference
Valiente-Banuet and Godínez-Alvarez
"
"
of stored water, apical growth, and the reproductive potential (McAuliffe and Janzen 1986). Interspecific competition between cacti and their nurse plants has been reported for C. gigantea and Cercidium microphyllum in the
Sonoran Desert (McAuliffe 1984b) and for Neobuxbaumia
tetetzo and Mimosa luisana in the Tehuacán Valley
(Valiente-Banuet et al. 1991b; Flores-Martínez et al. 1998).
The intensity of this interaction between cacti and their
nurse plants varies with the age of both interactants as well
as among dry and wet years, leading to the eventual elimination of either species depending on which had a competitive advantage (McAuliffe 1984b; Valiente-Banuet et al.
1991b; Flores-Martínez et al. 1998).
TA B L E 6 . 2 . (continued)
Species
Nurse plant(s)
Reference
M. microcarpa
Opuntia fulgida
McAuliffe (1984a)
M. thornberi
Celtis pallida
Lycium andersonii
L. berlandieri
Olneya tesota
Zizyphus obtusifolia
Suzán et al. (1996)
Neobuxbaumia tetetzo
Caesalpinia melanadenia
Castela tortuosa
Eupatorium odoratum
Valiente-Banuet et al. (1991a)
Opuntia leptocaulis
Larrea tridentata
Yeaton (1978)
Peniocereus greggii
Celtis pallida
Suzán et al. (1996)
P. striatus
Lycium andersonii
L. berlandieri
Olneya tesota
Zizyphus obtusifolia
Stenocereus thurberi
Haematoxylum brasiletto
Jatropha vernicosa
Tecoma stans
Arriaga et al. (1993)
Trichocereus pasacana
Aphyllocladus spartioides
Larrea divaricata
Prosopis ferox
De Viana (1997)
Tumamoca macdougallii
Celtis pallida
Lycium andersonii
L. berlandieri
Olneya tesota
Zizyphus obtusifolia
Suzán et al. (1996)
Reproduction and Breeding Systems
Among flowering plants, the Cactaceae have flowers that
are strongly associated with animals, such as bees, hawkmoths, hummingbirds, and bats (Porsch 1939; Grant and
Grant 1979; Gibson and Nobel 1986; Valiente-Banuet et al.
1996; Chapter 5). Plant-pollinator relationships are specialized for some cactus species, such as for opuntias with
large solitary, diurnal, bowl-shaped flowers adaptable to
bee pollination. Bee fauna in the Chihuahuan Desert of
Mexico, where opuntia species are highly diversified, is particularly rich with at least 90 species of bees visiting opuntia flowers in North America (Grant and Hurd 1979).
Other species pollinated by bees belong to tribe Pachycereeae, such as Myrtillocactus geometrizans.
Tubular, red, hummingbird-pollinated flowers are
"
common in Andean and tropical American cacti, especially in genera such as Rathbunia, Peniocereus, Nopalea, and
Pachycereus (Gibson and Nobel 1986). The hawkmothflower syndrome—characterized by strongly scented nightblooming flowers with white or whitish perianths and
long, slender nectar-containing floral tubes — occurs in
species of Acanthocereus, Cereus, Epiphyllum, Selenicereus,
and Trichocereus (Grant and Grant 1979). The bat-flower
syndrome—consisting of nocturnal anthesis, whitish bowlshaped flowers with a strong unpleasant odor at night,
large quantities of pollen and nectar, many anthers, and
large-mouthed and strong single flowers (Faegri and van
der Pijl 1979; Grant and Grant 1979) —occurs for about
70% of columnar cacti in tribe Pachycereeae (ValienteBanuet et al. 1995a, 1996). Most such species studied in
Population and Community Ecology
95
TA B L E 6 . 3
Pollinators of native columnar cacti (tribes Pachycereeae and Cereeae)
Locality
Extratropical, northwestern
Mexico
Tropical, southcentral
Mexico
Tropical, northwestern
Venezuela
Tropical, southern Peru
Species
Pollinator(s)
Carnegiea gigantea
Lophocereus schottii
Pachycereus pringlei
Stenocereus thurberi
Cephalocereus columna-trajani
Bees, bats, and birds
Moths
Bats, birds, and bees
Birds, bats, and bees
Bats
Mitrocereus fulviceps
Myrtillocactus geometrizans
Neobuxbaumia macrocephala
N. mezcalaensis
N. tetetzo
Pachycereus hollianus
Bats
Bees
Bats
Bats
Bats
Bats
P. marginatus
P. weberi
Pilosocereus chrysacanthus
Stenocereus pruinosus
Hummingbirds
Bats
Bats
Bats
S. stellatus
Pilosocereus lanuginosus
P. moritzianus
Stenocereus griseus
Subpilocereus horrispinus
S. repandus
Weberbauerocereus werberbaueri
Bats
Bats
Bats
Bats
Bats
Bats
Hummingbirds and bats
Reference
Alcorn et al. (1961)
Fleming and Holland (1998)
Fleming et al. (1996)
Alcorn et al. (1962)
A. Valiente-Banuet, Ma.
Del Arizmendi, A. Casas,
and A. Rojas-Martínez
(unpublished observations)
"
"
Valiente-Banuet et al. (1997a)
"
Valiente-Banuet et al. (1996)
S. Dar, A. Valiente-Banuet,
and Ma. Del Arizmendi
(unpublished observations)
"
Valiente-Banuet et al. (1997b)
"
V. Cortés, A. Valiente-Banuet,
and A. Rojas-Martínez
(unpublished observations)
Casas et al. (1999)
Petit (1995)
Nassar et al. (1997)
"
"
"
Sahley (1996)
All breeding systems are monoecious, except trioecious for P. pringlei and andredioecious for N. mezcalaensis.
North America are self-incompatible and produce fruits
only in presence of nectar-feeding bats (Table 6.3), such as
Choeronycteris mexicana, Leptonycteris curasoae, and L. nivalis (Valiente-Banuet et al. 1996, 1997a,b).Tabe6l3n.eahrere:
An apparent dichotomy occurs within and outside the
tropics among columnar cacti with “bat-pollinated”
flowers (Valiente-Banuet et al. 1997a,b), in which C. gigantea, P. pringlei, and S. thurberi at latitudes above 29º N
are pollinated by a wide spectrum of animals, including
birds, bats, and bees (Fleming et al. 1996). Fruit set for pollinators that are diurnal (birds and bees) versus nocturnal
(bats) for C. gigantea is 68% versus 40%. In contrast,
species in the tropics are self-incompatible and are pollinated exclusively by bats (Sosa and Soriano 1992; Valiente-
96
Valiente-Banuet and Godínez-Alvarez
Banuet et al. 1996, 1997a,b; Nassar et al. 1997; Casas et al.
1999). This predictability in pollinator availability is lower
in extratropical areas, where nectar-feeding bats are seasonal migrants from Arizona and northern Sonora to the
tropical deciduous forests of Sonora and Sinaloa (RojasMartínez et al. 1999). In contrast, Leptonycteris spp. have
resident populations within the tropics, e.g., in the
Tehuacán Valley (Rojas-Martínez and Valiente-Banuet
1996; Rojas-Martínez et al. 1999), as explained by the predictability of pollinators throughout the year (ValienteBanuet et al. 1997a,b; Rojas-Martínez et al. 1999). Anthesis
for most columnar cacti lasts about 12 hours in the intertropical deserts of Venezuela, 13 to 15 hours in Mexico,
and even longer (19–23 hours) for C. gigantea, P. pringlei,
and S. thurberi in the Sonoran Desert (Alcorn et al. 1961;
McGregor et al. 1959, 1962; Fleming et al. 1996; ValienteBanuet et al. 1996). An obligate pollination mutualism occurs for Lophocereus schottii and the moth Upiga virescens
in the Sonoran Desert (Fleming and Holland 1998).
The cactus family is ideal for studying breeding-system
evolution, because trioecy occurs for P. pringlei with males,
seed-producing females, and hermaphrodites (Fleming et
al. 1994), and androdioecy occurs for Neobuxbaumia mezcalaensis with male (female sterile) and hermaphrodite individual plants (Table 6.3; Valiente-Banuet et al. 1997a).
Trioecy and androdioecy are uncommon sexual systems;
androdioecy is uncommon because of the difficulty for
male (female sterile) plants to invade hermaphrodite populations (Charlesworth and Charlesworth 1978). Other
cactus species have dioecious or subdioecious breeding systems (i.e., with male or female sterile and hermaphrodite
plants). Four species of Opuntia (O. glausecens, O. grandis,
O. robusta, and O. stenopetala) are dioecious, two species of
Mammillaria are dioecious (M. dioica and M. neopalmeri),
and Selenicereus innesii is gynodioecious (Parfitt 1985; Del
Castillo 1986; Hoffman 1992).
Seed Dispersal
Seed dispersal is an important stage in the life cycle of
cacti; it can favor the success of seeds under the canopies
of nurse plants (Valiente-Banuet and Ezcurra 1991;
Godínez-Alvarez et al. 1999). The successful dispersal of
seeds to these sites can increase the area of distribution of
cacti and affects gene flow among populations (Howe and
Smallwood 1982). Information about the mechanisms by
which cactus seeds are dispersed under natural conditions
is scarce. In this regard, Bregman (1988), analyzing the
structural characteristics of fruits and seeds of more than
100 species of cacti, suggested that these plants can be dispersed by wind (anemochory), water (hydrochory), and
animals (zoochory).
Of possible mechanisms of seed dispersal, anemochory
and hydrochory are less studied. Anemochory has been
suggested for individuals of the genus Pterocactus, which
present dry fruits with winged seeds that are exposed when
the fruit dehisce at maturity (Bregman 1988). Hydrochory
has been suggested for seeds of the genera Astrophytum,
Discocactus, Frailea, Gymnocalycium, Matucana, and Thrixanthocereus. These cacti produce dry fruit containing relatively large seeds with a large hilum, a thin seed coat, and
a small embryo, which presumably favor the dispersal by
water (Bregman 1988).
Zoochory is the most common mode of transportation
of seeds reported for different species of cacti (Table 6.4).
Bregman (1988) suggested that seed dispersal by animals
occurred in three ways: (1) seeds could be transported passively on the outside of animals, epizoochory; (2) seeds
could be transported externally by an animal, synzoochory;
or (3) seeds could be consumed by animals, endozoochory.
Epizoochory is found only in epiphytic cacti of the genus
Rhipsalis, whose fruits contain sticky seeds that adhere to
the bill of birds feeding on the fruits. Synzoochory has
been suggested for cacti of the genera Opuntia, Parodia,
Blossfeldia, Krainzia, Strombocactus, and Aztekium, among
others. Seeds of these cacti are generally predated upon by
harvester ants (Pogonomyrmex spp., Messor spp.); however,
dispersal can also occur when the ants accidentally lose
seeds during transportation to their nests (Vargas-Mendoza
and González-Espinosa 1992).Tabe6l4.neahrere:
Endozoochory occurs for species of cacti that produce
fleshy fruits. This kind of fruit is found in most species in
the genera Opuntia, Epiphyllum, Hylocereus, Pachycereus,
Ferocactus, Melocactus, Carnegiea, Neobuxbaumia, Myrtillocactus, Stenocereus, Cephalocereus, Subpilocereus, and Pilosocereus, among others, and serves as an attractant to different groups of animals, such as reptiles, birds, and mammals
that consume the pulp and seeds (Steenbergh and Lowe
1977; Silva 1988; Wendelken and Martin 1988; León de la
Luz and Cadena 1991; Soriano et al. 1991; Vargas-Mendoza
and González-Espinosa 1992; Cortes Figueira et al. 1994;
Silvius 1995; Valiente-Banuet et al. 1996). Indeed, Opuntia
fruit were probably consumed by extinct megafauna about
10,000 years ago ( Janzen 1986).
In some cases, passage of seeds through vertebrate guts
increases germination, such as for Melocactus violaceus
(Cortes Figueira et al. 1994) and Stenocereus gummosus
(León de la Luz and Cadena 1991), whereas for others, seed
germination decreases or there is no effect, as for C. gigantea (Steenbergh and Lowe 1977), Neobuxbaumia tetetzo (H. Godínez-Alvarez, A. Rojas-Martínez, and A.
Valiente-Banuet, unpublished observations), Opuntia rastrera (Mandujano et al. 1997), and Stenocereus griseus
(Silvius 1995). The central questions for seed dispersal by
animals are to determine if animal vectors are effective dispersers that transport seeds to safe sites beneath nurse
plants and to establish whether seed dispersal has consequences on the maintenance of cactus populations under
natural conditions. Vargas-Mendoza and GonzálezEspinosa (1992) found that survival of seedlings of Opuntia
streptacantha during the first 5 months differed among microsites. Birds and bats consume the fruits of Neobuxbaumia tetetzo, but bats more effectively disperse viable
Population and Community Ecology
97
TA B L E 6 . 4
Seed dispersal by animals for cacti
Species
Seed dispersers
Carnegiea gigantea
Melocactus violaceus
Neobuxbaumia tetetzo
Opuntia rastrera
O. streptacantha
Pilosocereus maxonii
P. tillianus
Stenocereus eichlamii
S. griseus
S. gummosus
Subpilocereus repandus
Different species of insects,
birds, and mammals
Zenaida asiatica
Lizards
Different species of birds and bats
Different species of mammals and birds
Pogonomyrmex barbatus and
other species of rodents
Birds (18 spp.)
Glossophaga longirotris
Birds (18 spp.)
Glossophaga longirotris
Birds (14 spp.)
Different species of insects,
reptiles, birds, and mammals
Glossophaga longirotris
seeds to safe sites (H. Godínez-Alvarez, A. Rojas-Martínez,
and A. Valiente-Banuet, unpublished observations).
Population Dynamics
The maintenance of cactus populations under natural conditions is affected by various interactions with other organisms as well as by abiotic factors, such as rainfall and
solar radiation (Fig. 6.1). The assessment of the relative importance of each factor provides information on the main
causes that affect the population dynamics of these plants.
In this respect, matrix models are a useful tool to quantify the relative contribution made by different life stages to
the rate of population increase. In such demographic models, individuals are grouped into size categories, and a projection matrix is constructed according to the survival,
growth, and reproduction probabilities of the individuals
in each category. This projection matrix is formed by three
parts: (1) the first row includes the fecundity values for all
of the reproductive classes, (2) the main diagonal includes
the probabilities that individuals remain in the same size
class after one year, and (3) the first lower subdiagonal incorporates the probabilities that individuals grow to the
next size class after one year. The solution of this model
permits one to obtain the rate of population increase (the
largest positive eigenvalue), the stable size distribution
98
Valiente-Banuet and Godínez-Alvarez
Reference
Steenbergh and Lowe (1977)
Olin et al. (1989)
Cortes Figueira et al. (1994)
Valiente-Banuet et al. (1996);
H. Godínez-Alvarez, A. RojasMartínez, and A. Valiente-Banuet
(unpublished observations)
Mandujano et al. (1997)
Vargas-Mendoza and GonzálezEspinosa (1992)
Wendelken and Martin (1988)
Soriano et al. (1991)
Wendelken and Mattin (1988)
Soriano et al. (1991)
Silvius (1995)
León de la Luz and Cadena (1991)
Soriano et al. (1991)
(right eigenvector), and the reproductive values (left eigenvector; De Kroon et al. 1986). Moreover, elasticity can be
analyzed for the critical stages of the life cycle. Elasticity is
a relative measure, between 0 and 1 (or 0 and 100%), of the
change in the rate of population increase in response to
changes in matrix values (De Kroon et al. 1986).Fgiure6n1.eahrere:
Studies with this approach have been conducted with
the giant columnar cacti C. gigantea (Silvertown et al.
1993), Neobuxbaumia tetetzo (Godínez-Alvarez et al. 1999),
and P. pringlei. These studies show similar demographic
patterns in which the survivorship of young individuals
makes a higher contribution to the rate of population increase than does growth and reproduction of older plants
(Fig. 6.1). The elasticity values of survivorship varies
among species, from 86% for P. pringlei, 91% for N. tetetzo, to 99% for C. gigantea (Silvertown et al. 1993; GodínezAlvarez et al. 1999).
Elasticity analyses of N. tetetzo and P. pringlei indicate
that survivorship of immature and mature individuals have
the highest values, ranging from 20 to 60% (Fig. 6.2).
Survivorship of these categories is affected by competitive
interactions with other plants of the same and/or different
species (McAuliffe 1984b; McAuliffe and Janzen 1986;
Valiente-Banuet et al. 1991b; Flores-Martínez et al. 1998).
On the other hand, elasticity values for the rest of the cat-
Flower and bud predation
Pollination
Herbivores
Reproduction
Nurse plants
Seed production
Seed dispersal
Soil moisture
Germination
Growth
Intraspecific and interspecific
competition
Light (PPF)
Herbivores
Establishment
Nurse plants
Seedling predation
Soil moisture
Soil nutrients
Light (PPF)
Figure 6.1. Biotic and abiotic factors affecting the life cycle of cacti.
egories for both species vary between 5 and 10% (Fig. 6.2).
Although survivorship of N. tetetzo and P. pringlei seedlings
does not have a high elasticity value (5%), this life cycle
stage plays a significant role in the population dynamics
because their survival probabilities are low and highly variable (6 × 10-4 in N. tetetzo after 2 years, Godínez-Alvarez et
al. 1999; 0.01 in P. pringlei after 1 year, C. Silva-Pereyra, A.
Valiente-Banuet, L. Valiente, P. Dávila, and J. Ortega, unpublished observations). The main mortality factor for
seeds is predation by birds, rodents, and ants; for seedlings,
desiccation by direct solar irradiation is the principal mortality factor. The survivorship pattern of these cacti indicates a high mortality for seeds and seedlings and greater
survivorship for older individuals (a type III curve;
Steenbergh and Lowe 1969; Valiente-Banuet and Ezcurra
1991). This survivorship pattern has also been found for
other species of cacti, such as Ferocactus acanthodes ( Jordan
and Nobel 1981), F. cylindraceus (Bowers 1997), Lophocereus
schottii (Parker 1989), Mammillaria gaumeri (LeiranaAlcocer and Parra-Tabla 1999), and Stenocereus thurberi
(Parker 1987). Because the survivorship of seeds and
seedlings depends on the arrival of seeds to sites under
perennial plants (Fig. 6.1), the association of cacti with
nurse plants is an important interaction in the population
dynamics of cacti. The protection provided by nurse
plants decreases the probability of mortality of seeds and
seedlings, thereby increasing the probability that seedlings
grow to maturity (Turner et al. 1966; Steenbergh and Lowe
1969, 1977; Valiente-Banuet and Ezcurra 1991).Fgiure62.neahrere:
Elasticity analyses of N. tetetzo and P. pringlei also indicate that seed dispersal is an important interaction in the
population dynamics of cacti (Fig. 6.2). Frugivorous birds
and bats consume the fleshy fruits of different species of
cacti, such as C. gigantea (Steenbergh and Lowe 1977; Olin
et al. 1989) and N. tetetzo (Valiente-Banuet et al. 1996).
These fruit-eating animals can transport seeds directly to
the canopies of nurse plants, affecting the population dynamics of these cacti. Bats are actually better dispersers than
birds for N. tetetzo, because they transport intact and viable
seeds directly to the nurse plants, thereby maintaning a
continual flux of seeds to sites for seedling establishment.
Although demographic models indicate that biotic interactions are more significant than abiotic factors, soil
evolution plays a major role in the population dynamics of
P. pringlei (McAuliffe 1991; Valiente-Banuet et al. 1995a). In
alluvial landscapes, cacti have their densest populations in
young, coarse-textured soils (Parker 1989, 1995; McAuliffe
1991), with marginal populations in older alluvial soils that
have higher per capita mortality than those in younger deposits. These observations suggest declines from what originally were denser populations (McAuliffe 1991). In alluvial
regions, soils derive from the same source and usually share
the same climatic conditions, but pedogenetic processes
occur at different times, e.g., soils of alluvial fans and de-
Population and Community Ecology
99
Neobuxbaumia tetetzo
1
3
1
1
3
se
sa
j
im
m
5
7
9
20
50
Nurse plants
Intra- and interspecific
competition
Seed dispersal
Pachycereus pringlei
2
2
2
2
2
se
sa
j
im
m
5
6
8
6
60
Nurse plants
Survivorship
Growth
Reproduction
Figure. 6.2. Life-cycle diagram and elasticity values of two columnar cacti, Neobuxbaumia tetetzo and Pachycereus pringlei. Values are given in
percentages. Se = seedlings (0–15 cm in height); sa = saplings (15–45 cm); j = juvenile (45–100 cm); im = immature (100–150 cm); m = mature
(150–>650 cm). Values corresponding to seed germination and seedling establishment are incorportated in those for the reproduction of
mature individuals. References: for N. tetetzo, Godínez-Alvarez et al. 1999; for P. pringlei, C. Silva-Pereyra, A. Valiente-Banuet, L. Valiente,
J. Ortega-Ramírez, and P. Dávila, unpublished observations.
bris deposits become progressively more well developed
over time (Webb et al. 1987; McAuliffe 1994; Parker 1995;
Bowers 1997; Valiente-Banuet et al. 1995b). Older soils
usually contain well developed argillic horizons and calcium carbonate (CaCO3) accumulation in the Bk horizon,
both reducing water availability (Hennesy et al. 1983).
Thus cactus populations show different structures and
composition among different-aged soils, leading to major
differences in the population dynamics. For example, soil
evolution for P. pringlei in Baja California Sur reduces seed
production, seedling establishment, survival, and growth,
and populations in younger-soil deposits have positive
rates of increase (C. Silva-Pereyra, A. Valiente-Banuet, and
J. Ortega, unpublished observations). This pattern has also
been reported for other P. pringlei populations and for
Fouquieria columnaris (McAuliffe 1991). In all cases, soil geomorphic development in alluvial landscapes is a key to
spatial heterogeneity in plant population dynamics of cacti,
leading to a metapopulation approach (Fig. 6.3).Fgiure63n.eahrere:
100
Valiente-Banuet and Godínez-Alvarez
To understand the population dynamics of cacti, a hierarchial organization of biotic and abiotic factors should
be considered (Fig. 6.3). Biotic interactions may be the
main factors that affect cacti at a local population level,
whereas dispersion among populations and the effects of
soil types on recruitment become important at the
metapopulation level. Finally, the geomorphic history of
the localities in which populations of cacti occur should be
analyzed to understand population dynamics at the landscape level (Fig. 6.3).
Community Ecology
Cacti have great diversity in the Sonoran, Chihuahuan, and
Tehuacán-Cuicatlán deserts. Due to the relative uniformity of deserts and the apparently stressful conditions imposed upon desert species, early studies that were mostly autoecological have dominated the discussion about how
diversity is maintained (Noy-Meir 1973). However, since
1990 different studies have progressed toward an under-
LANDSCAPE
Geomorphic history that considers different events such as depositional
processes, formation of alluvial fans, and soil evolution.
METAPOPULATION
Dispersion among populations of the same species and patch
dynamics. Influence of different soil types on the recruitment
process. Differential effects of biotic interactions in different
patches.
LOCAL POPULATION
Interactions between species such as nurse plant
phenomenon, predation, seed dispersal, pollination, and
competition, among others.
Figure 6.3. Hierarchical organization of biotic and abiotic factors affecting the population dynamics of cacti.
standing about the role of biotic interactions on species coexistence in addition to the analysis of abiotic conditions.
Cactus-dominated communities support a surprisingly diverse fauna and flora that include hundreds of species
(Table 6.5). The classification systems of the vegetation of
Mexico (Miranda and Hernández 1963; Rzedowski 1978)
recognize different plant associations dominated by cacti:
Nopaleras, mostly in the Chihuahuan and the Sonoran
deserts, and “sarcocaulescent shrublands” including
Cardonales, Tetecheras, and “Stenocerales” dominated by
giant columnar cacti in the Tehuacán-Cuicatlán Valley, the
southern part of the Chihuahuan Desert in Querétaro and
Hidalgo, and the Sonoran Desert. Plant associations dominated by Carnegiea gigantea and Pachycereus pringlei occur
in the Sonoran Desert (Turner et al. 1995). Indeed, P.
pringlei is the most widely distributed dominant species in
Baja California. In the Tehuacán-Cuicatlán Valley, a total
of nine vegetation types are dominated by giant columnar
cacti (Osorio et al. 1996). In these ecosystems, trees and
shrubs, while modifying the microenvironmental conditions beneath their canopies, are controlling the availability of resources to other organisms by creating special habitats. Thus nurse-plant associations significantly contribute
to the establishment of nearly all cactus species, acting as
“ecosystem engineers” ( Jones et al. 1997) and playing a
major role in determining the structure and functioning of
desert ecosystems (Valiente-Banuet and Ezcurra 1991).
Species can concentrate beneath the canopies of nurse
plants—in some communities 60% of the species are
found there (Valiente-Banuet 1991). This affects intraspecific competition, as for Carnegiea gigantea (McAuliffe
and Janzen 1986), and interspecific competition (McAuliffe 1984; Valiente-Banuet et al. 1991b). Once a columnar cactus grows to more than 1 m in height, it may outcompete its nurse plant, defining a cyclical relationship in
which the association starts as a facilitation process and
Population and Community Ecology
101
TA B L E 6 . 5
Cactus-dominated ecosystems in Mexico and their species richness
Species richness
(number of plant species in 1,000 m2 quadrats)
Plants
Rodents
Bats
Birds
Reptiles
Opuntia rastrera, O. microdasys,
O. violacea
30
9
9
23
6
Tehuacán Valley,
Puebla
N. tetetzo,
Myrtillocactus geometrizans
46
4
34
91
—
Cephalocereus columna-trajani Cardonal
Tehuacán Valley,
Puebla
C. columna-trajani
54
4
34
91
—
Stenocereus stellatus Cardonal
Tehuacán Valley,
Puebla
S. stellatus, S. pruinosus,
Opuntia pilifera,
Myrtillocactus geometrizans
35
—
34
91
—
Pachycereus weberi Cardonal
Tehuacán Valley,
Puebla
P. weberi, Opuntia pilifera,
O. puberula,
Stenocereus pruinosus
48
4
34
91
—
Escontria chiotilla Chiotillal
Tehuacán Valley,
Puebla
E. chiotilla,
Stenocereus stellatus
34
4
34
91
—
Mitrocereus fulviceps Cardonal
Tehuacán Valley,
Puebla
M. fulviceps,
Echinocactus platyacanthus
45
—
34
91
—
Pachycerus pringlei Cardonal
Sonoran Desert,
Baja California Sur
P. pringlei
31
4
26
~60
—
Plant association
Locality
Dominant cacti
Chihuahuan Desert Nopalera
Mapimí,
Durango
Neobuxbaumia tetetzo Tetechera
Adapted from Barbault and Halffter (1981), Delhoume and Maury (1992), Arizmendi and Espinosa de los Monteros (1996), and Valiente-Banuet and Arizmendi (1998).
finishes as a competitive exclusion between the cactus and
its perennial nurse plant (Yeaton 1978; McAuliffe 1988).
Cyclical dynamics can be important in structuring desert
communities, and the process has been defined as
Markovian in community dynamics (McAuliffe 1988).
Indeed, complex combinations of interactions are widespread in desert communities, and the balance between facilitation and competition, which varies with life stage,
physiology, and environmental stresses, deserves further
research (Valiente-Banuet and Ezcurra 1991; Bertness and
Callaway 1994; Callaway and Walker 1997). For instance,
the population dynamics of P. pringlei established in welldeveloped Pleistocene alluvial soils may drift toward extinction, especially during extended dry periods, whereas
in young Holocene soils with more favorable moisture
conditions, biotic interactions have a central role along its
different life-cycle stages (C. Silva-Pereyra, A. ValienteBanuet, L. Valiente, P. Dávila, and J. Ortega, unpublished
observations).
Pollination and seed production are caused by a wide
array of animal vectors, such as bees, hummingbirds,
hawkmoths, and bats. In some high diversity areas, cacti
maintain different and diverse guilds of cactus pollinators,
such as about 90 species of bees that visit Opuntia flowers
in the Chihuahuan Desert (Grant and Hurd 1979) and 9
species of nectar-feeding bats in the columnar cactus
forests of south-central Mexico (Rojas-Martínez and
Valiente-Banuet 1996; Valiente-Banuet et al. 1996).
The nectar-feeding bat Leptonycteris curasoae is a latitudinal migrant throughout its distribution in North
America (Cockrum 1991; Fleming et al. 1993) and the most
important pollinator of columnar cacti (Valiente-Banuet et
al. 1996, 1997a,b). It has resident populations in the
Tehuacán Valley and the Balsas River Basin (RojasMartínez et al. 1999), and its pollination relationships with
columnar cacti are tight and coupled. In the northern limit
of its distribution (southwestern United States), L. curasoae
migrates during fall and winter, probably to the tropical
deciduous forests of Sonora, Mexico, following the seasonal availability of floral resources (Rojas-Martínez et al.
1999). The predominance of cactus-floral resources during
spring and summer in these ecosystems and during autumn and winter in tropical deciduous forests supports
high population densities of nectar- and fruit-feeding bats
(Rojas-Martínez and Valiente-Banuet 1996) and is correlated with the arrival of a great number of bird species that
consume fruits and seeds (Arizmendi and Espinosa de los
Monteros 1996). Indeed, bats play two important ecological roles by pollinating the flowers and dispersing the seeds
directly to safe sites. Those biotic interactions that increase
the average individual fitness of at least one species with no
negative effects on any other species are defined as positive
(Hacker and Gaines 1997). Positive biotic interactions apparently have the predominant effects on species diversity
in such ecosystems. However, more research needs to be
done in these environments to determine indirect effects of
species.
Conclusions and Future Prospects
At the population level, biotic interactions that cacti have
within and between trophic levels help determine survivorship, mortality, and fecundity patterns observed
under natural conditions. Although demographic models
indicate that positive interactions (i.e., facilitation) affect
the most sensitive life-cycle stages, the contribution of biotic and abiotic factors to the population dynamics of a
particular cactus species is poorly understood. Much research needs to be done to analyze the importance of a
metapopulation approach in which soils occupy a central
role (Fig. 6.3). Specifically, soil-geomorphic evolution is a
key aspect for evaluating the relative importance of abiotic components versus biotic interactions on demography.
Preliminary analyses indicate that soil evolution exerts a
strong influence on the predominance of cacti in arid environments.
At the community level, biotic interactions also occupy a central role in the explanation of how species coexistence is maintained. Positive interactions such as nurseplant associations, pollination mutualisms, and seed
dispersal strategies clearly suggest that biotic interactions
contribute to species maintenance. However, indirect interactions have received little research attention, and almost all investigations have addressed the role of pairwise
interactions on particular species. As multispecies systems,
cactus-dominated communities deserve research on topdown processes that consider the role of predators in regulating competitive effects among plants. Moreover, considering the extremely important structural dominance of
cacti and the trophic relationships between these plants
and animals, bottom-up effects have been suggested but
have never been properly analyzed.
Considering the economic, cultural, and religious importance of cacti in Mexico, understanding the factors that
control the abundance and distribution of cacti over arid
landscapes should be a major objective of ecological investigations in deserts. The information presented indicates that many aspects need to be considered to have a
more comprehensive knowledge about how to conserve
and to manage this highly diversified group of plants
(Chapter 8). Indeed, the protection of positive interactions
Population and Community Ecology
103
should be a high priority for maintaining these plants in
their natural environments.
Acknowledgments
The authors thank Fondo Mexicano para la Conservación
de la Naturaleza (Project A1–97/36), Dirección General de
Asuntos del Personal Académico (Project DGAPA-IN207798), and Instituto de Ecología UNAM for supporting
our studies and María del Coro Arizmendi for comments
on the manuscript. A.V.-B. dedicates this chapter to the
memory of Dr. Carlos Vázquez-Yanes.
Literature Cited
Alcorn, S. M., S. E. McGregor, and G. Olin. 1961. Pollination of Saguaro cactus by doves, nectar-feeding bats
and honey bees. Science 132: 1594–1595.
Alcorn, S. M., S. E. McGregor, and G. Olin. 1962.
Pollination requirements of the Organpipe cactus.
Cactus and Succulent Journal (U.S.) 34: 134–138.
Altesor, A., E. Ezcurra, and C. Silva. 1992. Changes in the
photosynthetic metabolism during the early ontogeny
of four cactus species. Acta Oecologica 13: 777–785.
Arias, S. 1993. Cactáceas: Conservación y diversidad en
México. Revista Mexicana de Historia Natural 44: 109–
115.
Arizmendi, M. C., and A. Espinosa de los Monteros. 1996.
Avifauna de los bosques de cactáceas columnares en el
Valle de Tehuacán. Acta Zoológica Mexicana (n.s.) 67:
25–46.
Arriaga, L., Y. Maya, S. Diaz, and J. Cancino. 1993.
Association between cacti and nurse perennials in a
heterogeneous tropical dry forest in northwestern
Mexico. Journal of Vegetation Science 4: 349–356.
Bregman, R. 1988. Forms of seed dispersal in Cactaceae.
Acta Botanica Neerlandica 37: 395–402.
Brum, G. D. 1973. Ecology of the saguaro (Carnegiea gigantea): Phenology and establishment in marginal
populations. Madroño 22: 195–204.
Callaway, R. M., and L. H. Walker. 1997. Competition
and facilitation: A synthetic approach to interactions in
plant communities. Ecology 78: 1958–1965.
Casas, A., A. Valiente-Banuet, A. Rojas-Martínez, and P.
Dávila. 1999. Reproductive biology and the process of
domestication of the columnar cactus Stenocereus stellatus in central Mexico. American Journal of Botany 86:
534–542.
Charlesworth, B., and D. Charlesworth. 1978. A model for
the evolution of dioecy and gynodioecy. American
Naturalist 112: 975–977.
Cockrum, E. L. 1991. Seasonal distribution of northwestern populations of the long nosed bats family
Phyllostomidae. Anales del Instituto de Biología. Serie
Zoología 62: 181–202.
Cortes-Figueira, J. E., J. Vasconcellos-Neto, M. A. García,
and A. L. Teixeira de Souza. 1994. Saurocory in
Melocactus violaceus (Cactaceae). Biotropica 26: 295–
301.
De Kroon, H., A. Plaiser, J. van Groenendael, and H.
Caswell. 1986. Elasticity: The relative contribution of
demographic parameters to population growth rate.
Ecology 67: 1427–1431.
Del Castillo, R. F. 1986. La selección natural de los sistemas
de cruzamiento en Opuntia robusta. Master’s Thesis,
Colegio de Postgraduados, Chapingo, Mexico.
Barbault, R., and G. Halffter. 1981. Ecology of the Chihuahuan Desert. Instituto de Ecología, Jalapa, Mexico.
Delhoume, J. P., and M. E. Maury. 1992. Actas del
Seminario Mapimí. Instituto de Ecología, Mexico City.
Barthlott, W., and D. R. Hunt. 1993. Cactaceae. In The
Families and Genera of Vascular Plants. Vol. III,
Flowering Plants, Dicotyledons (K. Kubitzk, J. G.
Rohwer, and V. Bittrich, eds.). Springer-Verlag, Berlin.
Pp. 161–197.
De Viana, M. L. 1997. Distribución espacial de Trichocereus pasacana (Cactaceae) en relación al espacio
disponible y al banco de semillas. Revista de Biología
Tropical 44: 95–103.
Bertness, M. D., and R. M. Callaway. 1994. Positive interactions in communities. Trends in Ecology and
Evolution 9: 191–193.
104
ed. Universidad Nacional Autónoma de México,
Mexico City.
Dubrovsky, J. G. 1996. Seed hydration memory in
Sonoran Desert cacti and its ecological implication.
American Journal of Botany 83: 624–632.
Bowers, J. E. 1997. Demographic patterns of Ferocactus
cylindraceus in relation to substrate age and grazing history. Plant Ecology 133: 37–48.
Dubrovsky, J. G. 1998. Discontinuous hydration as a facultative requirement for seed germination in two cactus species of the Sonoran Desert. Journal of the Torrey
Botanical Society 125: 33–39.
Bravo-Hollis, H. 1978. Las Cactáceas de México, Vol. 1, 2nd
Faegri, K., and L. van der Pijl. 1979. The Principles of
Valiente-Banuet and Godínez-Alvarez
Pollination Ecology, 3rd rev. ed. Pergamon Press,
Oxford.
Fleming, T. H., R. A. Núñez, and S. L. da S. Lobo. 1993.
Seasonal changes in the diets of migrant and non-migrant nectarivorous bats as revealed by carbon isotope
analysis. Oecologia 94: 72–75.
Fleming, T. H., S. Maurice, S. L. Buchmann, and M. D.
Tuttle. 1994. Reproductive biology and relative male
and female fitness in a trioecious cactus, Pachycereus
pringlei (Cactaceae). American Journal of Botany 81:
858–867.
Fleming, T. H.., M. D. Tuttle, and M. A. Horner. 1996.
Pollination biology and the relative importance of nocturnal and diurnal pollinators in three species of
Sonoran Desert columnar cacti. The Southwestern
Naturalist 41: 257–269.
Hacker, S. D., and S. D. Gaines. 1997. Some implications
of direct positive interactions for community species
diversity. Ecology 78: 1990–2003.
Hennesy, J. T., R. P. Gibbens, J. M. Tromble, and M.
Cardenas. 1983. Water properties of caliche. Journal of
Range Management 36: 723–726.
Hoffman, M. T. 1992. Functional dioecy in Echinocereus
coccineus (Cactaceae): Breeding system, sex ratios and
geographical range of sexual dimorphism. American
Journal of Botany 79: 1382–1388.
Howe, H. F., and J. Smallwood. 1982. Ecology of seed dispersal. Annual Review of Ecology and Systematics 13:
201–228.
Hutto, R. L., J. R. McAuliffe, and L. Hogan. 1986.
Distributional associates of the saguaro (Carnegiea gigantea). The Southwestern Naturalist 31: 469–476.
Fleming, T. H., and J. N. Holland. 1998. The evolution of
obligate pollination mutualisms: Senita cactus and
senita moth. Oecologia 114: 368–375.
Janzen, D. H. 1986. Chihuahuan Desert nopaleras: Defaunated big mammal vegetation. Annual Review of
Ecology and Systematics 17: 595–636.
Flores-Martínez, A., E. Ezcurra, and S. Sánchez-Colón.
1998. Water availability and the competitive effect of a
columnar cactus on its nurse plant. Acta Oecologica 19:
1–8.
Jones, C. G., J. H. Lawton, and M. Shachak. 1997. Positive
and negative effects of organisms as physical ecosystem
engineers. Ecology 78: 1946–1957.
Franco, A. C., and P. S. Nobel. 1989. Effect of nurse plants
on the microhabitat and growth of cacti. Journal of
Ecology 77: 870–886.
García-Moya, E., and C. M. McKell. 1970. Contribution
of shrubs to the nitrogen economy of a desert-wash
plant community. Ecology 51: 81–88.
Gibson, A. C., and P. S. Nobel. 1986. The Cactus Primer.
Harvard University Press, Cambridge, Massachusetts.
Godínez-Alvarez, H., and A. Valiente-Banuet. 1998.
Germination and early seedling growth of Tehuacan
Valley cacti species: The role of soils and seed ingestion
by dispersers on seedling growth. Journal of Arid
Environments 39: 21–31.
Godínez-Alvarez, H., A. Valiente-Banuet, and B. L.
Valiente. 1999. Biotic interactions and the population
dynamics of the long-lived columnar cactus Neobuxbaumia tetetzo in the Tehuacán Valley, Mexico.
Canadian Journal of Botany 77: 203–208.
Jordan, P. W., and P. S. Nobel. 1981. Seedling establishment
of Ferocactus acanthodes in relation to drought. Ecology
62: 901–906.
Kigel, J. 1995. Seed germination in arid and semi-arid regions. In Seed Development and Germination ( J. Kigel
and G. Galili, eds.). Marcel Dekker, New York. Pp.
645–699.
Leirana-Alcocer, J., and V. Parra-Tabla. 1999. Factors
affecting the distribution, abundance and seedling
survival of Mammillaria gaumeri, an endemic cactus of
coastal Yucatán, México. Journal of Arid Environments
41: 421–428.
León de la Luz, J. L., and R. D. Cadena. 1991. Evaluación
de la reproducción por semilla de la pitaya agria
(Stenocereus gummosus) en Baja California Sur, México.
Acta Botánica Mexicana 14: 75–87.
Mandujano, M. del C., J. Golubov, and C. Montaña.
1997. Dormancy and endozoochorous dispersal of
Opuntia rastrera seeds in the southern Chihuahuan
Desert. Journal of Arid Environments 36: 259–266.
Grant, V., and K. A. Grant. 1979. The pollination spectrum in the southwestern American cactus flora. Plant
Systematics and Evolution 133: 29–37.
McAuliffe, J. R. 1984a. Prey refugia and the distributions
of two Sonoran Desert cacti. Oecologia 65: 82–85.
Grant, V., and P. D. Hurd. 1979. Pollination of the southwestern Opuntias. Plant Systematics and Evolution 133:
15–28.
McAuliffe, J. R. 1984b. Sahuaro-nurse tree associations in
the Sonoran Desert: Competitive effects of sahuaros.
Oecologia 64: 319–321.
Population and Community Ecology
105
McAuliffe, J. R., and F. J. Janzen. 1986. Effects of
intraspecific crowding on water uptake, water storage,
apical growth, and reproductive potential in the
sahuaro cactus, Carnegiea gigantea. Botanical Gazette
147: 334–341.
McAuliffe, J. R. 1988. Markovian dynamics of simple and
complex desert plant communities. The American
Naturalist 131: 459–490.
McAuliffe, J. R. 1991. Demographic shifts and plant succession along a late Holocene soil chronosequence in
the Sonoran Desert of Baja California. Journal of Arid
Environments 20: 165–178.
McAuliffe, J. R. 1994. Landscape evolution, soil formation,
and ecological patterns and processes in Sonoran
Desert bajadas. Ecological Monographs 64: 111–148.
McGregor, S. C., S. M. Alcorn, E. B. Kurtz, and G. D.
Butler. 1959. Bee visitors to saguaro flowers. Journal of
Economic Entomology 52: 1002–1004.
McGregor, S. C., S. M. Alcorn, E. B. Kurtz, and G. Olin.
1962. Pollination and pollinating agents of the saguaro.
Ecology 43: 259–267.
Miranda, F., and E. X. Hernández. 1963. Los tipos de vegetación de México y su clasificación. Boletín de la
Sociedad Botánica de México 28: 29–179.
Nassar, J. M., N. Ramírez, and O. Linares. 1997.
Comparative pollination biology of Venezuelan columnar cacti and the role of nectar feeding bats in their sexual reproduction. American Journal of Botany 84: 918–
927.
Nobel, P. S. 1988. Environmental Biology of Agaves and
Cacti. Cambridge University Press, New York.
Nolasco, H., F. Vega-Villasante, H. L. Romero-Schmidt,
and A. Díaz-Rondero. 1996. The effects of salinity,
acidity, light and temperature on the germination of
seeds of cardón (Pachycereus pringlei (S. Wats.) Britton
and Rose, Cactaceae). Journal of Arid Environments 33:
87–94.
Parfitt, B. D. 1985. Dioecy in North American Cactaceae:
A review. Sida 11: 200–206.
Parker, K. C. 1987. Site-related demographic patterns of
organ pipe cactus populations in southern Arizona.
Bulletin of the Torrey Botanical Club 114: 149–155.
Parker, K. C. 1989. Height structure and reproductive characteristics of senita, Lophocereus schottii (Cactaceae), in
southern Arizona. The Southwestern Naturalist 34:
392–401.
Parker, K. C. 1993. Climatic effects on regeneration trends
for two columnar cacti in the northern Sonoran
Desert. Annals of the Association of American Geographers 83: 452–474.
Parker, K. C. 1995. Effects of complex geomorphic history
on soil and vegetation patterns on arid alluvial fans.
Journal of Arid Environments 30: 19–39.
Petit, S. 1995. The pollinators of two species of columnar
cacti on Curaçao, Netherlands Antilles. Biotropica 27:
538–541.
Porsch, O. 1939. Das Bestäubungsleben der Kakteenblüte,
II. Cactaceae. Jahrbuch der Deutsche KakteenGesellschaft II: 81–142.
Rojas-Aréchiga, M., A. Orozco-Segovia, and C. VázquezYanes. 1997. Effect of light on the germination of seven
species of cacti from the Zapotitlán Valley in Puebla,
México. Journal of Arid Environments 36: 571–578.
Rojas-Aréchiga, M., C. Vázquez-Yanes, and A. OrozcoSegovia. 1998. Seed response to temperature of Mexican cacti species from two life forms: An ecophysiological interpretation. Plant Ecology 135: 207–214.
Rojas-Aréchiga, M., and C. Vázquez-Yanes. 2000. Cactus
seed germination: A review. Journal of Arid Environments 44: 85–104.
Nolasco, H., F. Vega-Villasante, and A. Díaz-Rondero.
1997. Seed germination of Stenocereus thurberi
(Cactaceae) under different solar irradiation levels.
Journal of Arid Environments 36: 123–132.
Rojas-Martínez, A., and A. Valiente-Banuet. 1996. Análisis
comparativo de la quiropterofauna del Valle de
Tehuacán-Cuicatlán, Puebla-Oaxaca. Acta Zoológica
Mexicana (n.s.) 67: 1–22.
Noy-Meir, I. 1973. Desert ecosystems: environment and
producers. Annual Review of Ecology and Systematics 5:
25–51.
Rojas-Martínez, A., A. Valiente-Banuet, Ma. Del C.
Arizmendi, A. Alcántara-Egúren, and H. Arita. 1999.
Seasonal distribution of the long-nosed bat
(Leptonycteris curasoae) in North America: Does a generalized migration pattern really exist? Journal of
Biogeography 26: 1065–1078.
Olin, G., S. M. Alcorn, and J. M. Alcorn. 1989. Dispersal
of viable saguaro seeds by white-winged doves (Zenaida
asiatica). The Southwestern Naturalist 34: 282–284.
106
Osorio, B. O., A. Valiente-Banuet, P. Dávila, and R.
Medina. 1996. Tipos de vegetación y diversidad β en el
Valle de Zapotitlán de las Salinas, Puebla, México.
Boletín de la Sociedad Botánica de México 59: 35–58.
Valiente-Banuet and Godínez-Alvarez
Rzedowski, J. 1978. Vegetación de México. Editoral Limusa,
Mexico City.
Sahley, C. T. 1996. Bat and hummingbird pollination of an
autotetraploid columnar cactus, Weberbaurocereus weberbaueri (Cactaceae). American Journal of Botany 83:
1329–1336.
Shreve, F. 1951. Vegetation and Flora of the Sonoran Desert,
Vol. 1. Carnegie Institution of Washington, Washington, D.C.
Silva, W. R. 1988. Ornitocoria em Cereus peruvianus
(Cactaceae) na Serra do Japi, Estado de São Paulo.
Revista Brasileira de Biologia 48: 381–389.
Silvertown, J. W., M. Franco, I. Pisanty, and A. Mendoza.
1993. Comparative plant demography—relative importance of life-cycle components to the finite rate of
increase in woody and herbaceous perennials. Journal
of Ecology 81: 465–476.
Silvius, K. M. 1995. Avian consumers of cardón fruits
(Stenocereus griseus: Cactaceae) on Margarita Island,
Venezuela. Biotropica 27: 96–105.
Soriano, P. J., M. Sosa, and O. Rossell. 1991. Hábitos alimentarios de Glossophaga longirostris Miller
(Chiroptera: Phyllostomidae) en una zona árida de los
Andes venezolanos. Revista de Biología Tropical 39:
263–268.
Sosa, M., and P. Soriano. 1992. Los murciélagos y los cactus: una relación muy estrecha. Carta Ecológica 61: 7–
10.
Steenbergh, W. H., and C. H. Lowe. 1969. Critical factors
during the first years of life of the saguaro (Cereus giganteus) at the Saguaro National Monument, Arizona.
Ecology 50: 825–834.
Steenbergh, W. H., and C. H. Lowe. 1977. Ecology of the
Saguaro. II. Reproduction, Germination, Establishment,
Growth and Survival of the Young Plant. National Park
Service Scientific Monograph Series No. 8, U.S.
Government Printing Office, Washington, D.C.
Suzán, H., G. Nabhan, and D. T. Patten. 1996. The importance of Olneya tesota as a nurse plant in the
Sonoran Desert. Journal of Vegetation Science 7: 635–
644.
Turner, R. M., S. Alcorn, G. Olin, and J. A. Booth. 1966.
The influence of shade, soil and water on saguaro
seedling establishment. Botanical Gazette 127: 95–102.
Turner, R. M., J. E. Bowers, and T. L. Burgess. 1995.
Sonoran Desert Plants: An Ecological Atlas. University of
Arizona Press, Tucson.
Valiente-Banuet, A. 1991. Dinámica del establecimiento de
cactáceas: patrones generales y consecuencias de los
procesos de facilitación por plantas nodrizas en desiertos. Ph.D. Thesis, Universidad Nacional Autónoma de
México, Mexico City.
Valiente-Banuet, A., O. Briones, A. Bolongaro-Crevenna,
E. Ezcurra, M. Rosas, H. Núñez, G. Barnard, and E.
Vázquez. 1991a. Spatial relationships between cacti and
nurse shrubs in a semi-arid environment in central
Mexico. Journal of Vegetation Science 2: 15–20.
Valiente-Banuet, A., F. Vite, and A. Zavala-Hurtado.
1991b. Interaction between the cactus Neobuxbaumia
tetetzo and the shrub Mimosa luisana. Journal of
Vegetation Science 2: 11–14.
Valiente-Banuet, A., and E. Ezcurra. 1991. Shade as a cause
of the association between the cactus Neobuxbaumia
tetetzo and the nurse plant Mimosa luisana in the
Tehuacán Valley, Mexico. Journal of Ecology 79: 961–
971.
Valiente-Banuet, A., P. Dávila, M. C. Arizmendi, A. RojasMartínez, and A. Casas. 1995a. Bases ecológicas del desarrollo sustentable en zonas áridas: el caso de los
bosques de cactáceas columnares en el Valle de
Tehuacán y Baja California Sur, México. In IV Curso
sobre Desertificación y Desarrollo Sustentable en América
Latina y el Caribe (G. M. Anaya and C. S. F. Díaz,
eds.). PNUMA-FAO, Mexico City. Pp. 20–36.
Valiente-Banuet, A., P. Dávila, R. J. Ortega, M. C.
Arizmendi, J. L. León, A. Breceda, J. Cancino. 1995b.
Influencia de la evolución de una pendiente de piedemonte en una vegetación de cardonal de Pachycereus
pringlei en Baja California Sur, México. Investigaciones
Geográficas 3: 101–113.
Valiente-Banuet, A., M. C. Arizmendi, A. Rojas-Martínez,
and L. Domínguez-Canseco. 1996. Ecological relationships between columnar cacti and nectar feeding
bats in Mexico. Journal of Tropical Ecology 12: 103–119.
Valiente-Banuet, A., A. Rojas-Martínez, M. C. Arizmendi,
and P. Dávila. 1997a. Pollination biology of two columnar cacti (Neobuxbaumia mezcalaensis and Neobuxbaumia macrocephala) in the Tehuacan Valley, central
Mexico. American Journal of Botany 84: 452–455.
Valiente-Banuet, A., A. Rojas-Martínez, A. Casas, M. C.
Arizmendi, and P. Dávila. 1997b. Pollination biology of
two-winter-blooming giant columnar cacti in the
Tehuacan Valley, central Mexico. Journal of Arid
Environments 37: 331–341.
Population and Community Ecology
107
Valiente-Banuet, A., and M. C. Arizmendi. 1998. El escenario ecológico del Valle de Tehuacán-Cuicatlán. In
Tehuacan, Horizonte del Tiempo. Club Rotario
Tehuacán Manantiales, Mexico City. Pp. 45–61.
Vargas-Mendoza, M. C., and M. González-Espinosa. 1992.
Habitat heterogeneity and seed dispersal of Opuntia
streptacantha (Cactaceae) in nopaleras of central
Mexico. The Southwestern Naturalist 37: 379–385.
Webb, R. H., J. W. Steiger, and R. M. Turner. 1987.
108
Valiente-Banuet and Godínez-Alvarez
Dynamics of Mojave desert shrub assemblages in the
Panamint Mountains, California. Ecology 68: 478–490.
Wendelken, P. W., and R. F. Martin. 1988. Avian consumption of the fruit of the cacti Stenocereus eichlamii
and Pilosocereus maxonii in Guatemala. The American
Midland Naturalist 119: 235–243.
Yeaton, I. R. 1978. A cyclical relationship between Larrea
tridentata and Opuntia leptocaulis in the northern
Chihuahuan Desert. Journal of Ecology 66: 651–656.
CHAPTER
›7 ‹
C O N S U M P T I O N O F P L AT YO P U N T I A S
B Y W I L D V E RT E B R AT E S
Eric Mellink and Mónica E. Riojas-López
Introduction
Consumption of Vegetative Structures
Cladode Preferences
Seasonal Effects for Herbivory
Nutritional Qualitites of Cladodes
Spine Effects on Herbivory
Consumption of Reproductive Structures
Flower Consumption
Fruit Consumption
Evolutionary and Ecological Context
Past and Present Herbivory Pressures
Galápagos Islands
Alienized Relations
Concluding Remarks
Literature Cited
Introduction
In arid lands, platyopuntias (prickly pear cacti, also called
nopales [singular, nopal ]) often appear as lush, exhuberant
forage and, during the proper time of the year, bear
brightly colored fruits, which, as Gibson and Nobel (1986)
indicate, “would be a welcome waterhole and restaurant
for animals.” Cladodes and fruits of platyopuntias are indeed consumed by a variety of vertebrates in ways that
affect entire ecosystems (Anthony 1954; Janzen 1986).
This chapter presents an overview of the utilization of
platyopuntias by wild vertebrates. It also includes a list of
the vertebrate species reported to be consumers of platyopuntias. Certainly this list is not complete, as many con-
sumers have not been reported in the formal literature.
Because of the natural distribution of platyopuntias, the
information about vertebrate consumption is biased toward the deserts of North America and the Galápagos
Islands. Most sources indicating consumption of platyopuntias by vertebrates present rather incidental information on the issue; only a few, notably those for the
Galápagos Islands, derive from the study of ecological relations involving platyopuntias. Eighty-nine vertebrate
species appear in the literature as consumers of platyopuntias: 9 species of reptiles, 26 of birds, and 54 of mammals (Table 7.1). Pads (cladodes) and fruits are the parts
most often reported as consumed, although reports on the
use of fruits may mask the use of seeds.Tabe7ln1.eahrere:
109
TA B L E 7 . 1
A summary of wild, opuntiofagous vertebrates
Species
Part(s) consumed
Reference(s)
Reptiles
Berlandier’s tortoise (Gopherus berlandieri)
Galápagos tortoise (Testudo elephantinus)
Leopard tortoise (Geochelone pardalis)
Galápagos land iguana (Conocephalus pallidus)
Galápagos land iguana (C. subcristatus)
Daphne lava lizard (Microlophus albemarensis)
Pinta lava lizard (M. pacificus)
Whiptail (Cnemidophorus lemniscatus)
Lagarto Tizón (Gallotia galloti)
Pads, petals, fruits
Pads, fruits
Fruits
Pads, petals, fruits
Pads, petals
Petals, nectar, pollen
Petals, nectar, pollen,
fruits
Petals
Fruits
Auffenberg and Weaver (1969); Rose and Judd (1982)
Thorton (1971); Biggs (1990)
Milton (1992)
Christian et al. (1984)
Thorton (1971); Biggs (1990)
East (1995)
Schluter (1984)
Mijares-Urrutia et al. (1997)
Valido and Nogales (1994)
Birds
Ostrich (Struthio camelus)
Emu (Dromiceus sp.)
Wild turkey (Meleagris gallopavo)
Scaled quail (Callipepla squamata)
Northern bobwhite (Colinus virginianus)
White-winged dove (Zenaida asiatica)
Fruits
Fruits
Fruits
Fruits
Seeds
Fruits
Galápagos dove (Z. galapagoensis)
Mourning dove (Z. macroura)
Greater roadrunner (Geococcyx californianus)
Northern flicker (Colaptes auratus)
Pads, petals, nectar,
pollen, fruits, seeds
Fruits
Fruits
Fruits
Mexican jay (Aphelocoma ultramarina)
Black magpie (Gymnorhina tibicen)
Common raven (Corvus corax)
Chihuahuan raven (C. cryptoleucus)
Fruits
Fruits
Fruits
Fruits
Little raven (C. mellori)
Curve-billed thrasher (Toxostoma curvirostre)
Fruits
Fruits
Galápagos mockingbird (Nesomimus parvulus)
Pads, nectar,
pollen, seeds
Fruits
Fruits
Pads, nectar,
pollen, seeds
Nectar, pollen, seeds
Nectar, pollen, seeds
Nectar, pollen, seeds
Nectar, pollen, seeds
Pads, nectar, pollen,
fruits, seeds
Canyon towhee (Pipilo fuscus)
House finch (Carpodacus mexicanus)
Genovesa cactus finch (Geospiza conirostris)
Galápagos finch (G. difficilis)
Daphne Major ground finch (G. fortis)
Galápagos finch (G. fuliginosa)
Galápagos finch (G. magnirostris)
Daphne Major cactus finch (G. scandens)
Burt-Davy (1920)
Darnell-Smith (1919)
Lehman (1984)
Lehman (1984); Brown (1989)
Lehman (1984)
Lehman (1984); Gonzalez-Espinoza and QuintanaAscencio (1986)
Grant and Grant (1979, 1981)
Lehman (1984)
Clements and Shelford (1939)
Clements and Shelford (1939); Gonzalez-Espinoza and
Quintana-Ascencio (1986)
Gonzalez-Espinoza and Quintana-Ascencio (1986)
Darnell-Smith (1919)
M. Riojas-López (unpublished observations)
Lehman (1984); Gonzalez-Espinoza and QuintanaAscencio (1986)
McCulloch (1980)
Lehman (1984); Gonzalez-Espinoza and QuintanaAscencio (1986)
Grant and Grant (1981)
Gonzalez-Espinoza and Quintana-Ascencio (1986)
"
Grant and Grant (1981, 1989)
Bowman (1961); Grant and Grant (1981)
Grant and Grant (1981); Price (1987); Grant (1996)
Grant and Grant (1981); Dodd and Brady (1988)
Grant and Grant (1981)
Lack (1947); Bowman (1961); Grant and Grant
(1981); Millington and Grant (1983); Grant (1996)
TA B L E 7 . 1 (continued)
Species
Part(s) consumed
Reference
Mammals
Mexican long-tongued bat (Choeronycteris mexicana)
Big long-nosed bat (Leptonycteris nivalis)
Baboon (Papio hamadryas)
“Monkeys” (Family Cercopithecidae)
Shasta ground sloth (Nothrotheriops shastense)
Nectar, pollen
Fruits
Fruits
Fruits
Pads, petals, fruits
European rabbit (Oryctolagus cuniculus)
Desert cottontail (Sylvilagus audubonii)
Fruits
Pads, fruits
Nuttall’s cottontail (S. nuttallii)
Antelope jackrabbit (Lepus alleni)
Black-tailed jackrabbit (L. californicus)
Pads
Pads
Stems, pads, fruits
White-tailed jackrabbit (L. townsendii)
Pads
Harris’ antelope-squirrel (Ammospermophilus harrisii)
Spotted ground squirrel (Spermophilus spilosoma)
Thirteen-lined ground squirrel (S. tridecemlineatus)
Rock squirrel (S. variegatus)
Ground squirrel (S. sp.)
Botta’s pocket gopher (Thomomys bottae)
Mountain pocket gopher (T. talpoides)
Baileyi’s pocket mouse (Chaetodipus baileyi)
Hispid pocket mouse (C. hispidus)
Nelson’s pocket mouse (C. nelsoni)
Southern banner-tailed kangaroo rat (Dipodomys phillipsii)
Banner-tailed kangaroo rat (D. spectabilis)
Mexican spiny pocket mouse (Liomys irroratus)
Galápagos rice rat (Oryzomys galapagoensis)
Rock mouse (Peromyscus difficilis)
Pinyon mouse (P. truei)
Deer mouse (P. sp.)
Hispid cotton rat (Sigmodon hispidus)
White-throated packrat (Neotoma albigula)
Fruits, seeds
Pads
Fruits, seeds
Fruits
Seeds
Roots, stems
Pads
Seeds
Seeds
Fruits
Fruits
Pads
Fruits
Seeds
Fruits
Fruits
Seeds
Pads
Pads, fruits
Goldman’s packrat (N. goldmani)
Desert packrat (N. lepida)
Black rat (Rattus rattus)
North American porcupine (Erethizon dorsatum)
Coyote (Canis latrans)
Fruits
Pads
Seeds
Fruits
Fruits
Gray fox (Urocyon cinereoargenteus)
American black bear (Ursus americanus)
Fruits
Pads, fruits
Ringtail (Bassariscus astutus)
Fruits
Dalquest (1953)
Gonzalez-Espinoza and Quintana-Ascencio (1986)
Weed Section (1940)
Weed Section (1940)
Long et al. (1974); Hansen (1978); Spaulding
and Martin (1979)
Darnell-Smith (1919)
Gonzalez-Espinoza and Quintana-Ascencio (1986);
Hoffman et al. (1993)
MacCracken and Hansen (1984)
Vorhies and Taylor (1933)
Vorhies and Taylor (1933); Timmons (1942); Riegel
(1941); MacCracken and Hansen (1984); Hoffmeister
(1986); Hoffman et al. (1993); E. Mellink and
M. Riojas-López (unpublished observations)
Bear and Hansen (1966); Flinders and Hansen (1972),
cited in Dunn et al. (1982)
Hoffmeister (1986)
Bailey (1931)
Riegel (1941); Lehman (1984)
Hoffmeister (1986)
Janzen (1986)
Hoffmeister (1986)
Vaughan (1967)
Hoffmeister (1986)
Blair (1937)
Gonzalez-Espinoza and Quintana-Ascencio (1986)
"
Vorhies and Taylor (1922)
Gonzalez-Espinoza and Quintana-Ascencio (1986)
Thorton (1971)
Dalquest (1953)
"
Janzen (1986)
Lehman (1984)
Vorhies and Taylor (1940); Dalquest (1953); GonzalezEspinoza and Quintana-Ascencio (1986); Rangel and
Mellink (1993)
Gonzalez-Espinoza and Quintana-Ascencio (1986)
Hoffmeister (1986)
Eliasson (1968)
Hoffmeister (1986)
Lehman (1984); Gonzalez-Espinoza and QuintanaAscencio (1986); McLure et al. (1995)
Gonzalez-Espinoza and Quintana-Ascencio (1986)
Hoffmeister (1986); McClinton et al. (1992);
Hellgren (1993)
Leopold (1959); Hoffmeister (1986)
(continued on next page)
TA B L E 7 . 1 (continued )
Mammals (continued)
Species
Part(s) consumed
Reference
Raccoon (Procyon lotor)
Fruits
White-nosed coati (Nasua narica)
North American badger (Taxidea taxus)
Hooded skunk (Mephitis macroura)
Mammoth (Mammuthus sp.)
Feral burro (Equus asinus)
Wild boar (Sus scrofa)
Collared peccary (Pecari tajacu)
Fruits
Fruits
Fruits
Fruits
Stems, pads
Pads
Pads, petals, fruits
Camel (Camelus dromedarius)
Mule deer (Odocoileus hemionus)
Pads
Pads, fruits
White-tailed deer (O. virginianus)
Pads, fruits
Pronghorn (Antilocapra americana)
Pads, fruits
American bison (Bison bison)
Bighorn sheep (Ovis canadensis)
Fruits
Pads, fruits
Steenbok (Raphicerus campestris)
Feral goat (Capra hircus)
Pads
Stems, pads
Dalquest (1953); Gonzalez-Espinoza and QuintanaAscencio (1986); Lehman (1984)
Hoffmeister (1986)
Gonzalez-Espinoza and Quintana-Ascencio (1986)
"
Davis et al. (1984)
Krausman et al. (1989); Hicks andMauchamp (1995)
Baber and Coblentz (1987)
Dalquest (1953); Eddy (1961); Zervanos and Hadley
(1973); Everitt et al. (1981); Bissonette (1982); Sowls
(1984); Corn and Warren (1985); Gonzalez-Espinoza
and Quintana-Ascencio (1986); Hoffmeister (1986);
Janzen (1986); Luévano et al. (1991); MartinezRomero and Mandujano (1995)
Janzen (1986)
Krausman (1978); Janzen (1986); Krausman
et al. (1989)
Quinton and Horejst (1977); Arnold and Drawe
(1979); Everitt and Gonzalez (1979); Quinton et al.
(1979); Everitt et al. (1981); Gonzalez-Espinoza and
Quintana-Ascencio (1986); Hoffmeister (1986);
Luévano et al. (1991)
Russell (1964); Stelfox and Vriend (1977); Sexson et al.
(1981); Janzen (1986)
Janzen (1986)
Hoffmeister (1986); Dodd and Brady (1988);
Krausman et al. (1989)
Marais (1939)
Eliasson (1968); Thorton (1971); Hicks and
Mauchamp (1995)
Consumption of Vegetative Structures
Four of the nine reptiles that forage on nopales consume
their cladodes (pads). These include two tortoises and two
land iguanas (scientific names also in Table 7.1). Only 16%
of bird species consume the pads, but at least 43% of mammal species, including the Pleistocene Shasta ground sloth
and mammoth, do, or did, so. Consumption of vegetative
structures is mostly restricted to the pads for mammals, and
exclusively so for reptiles (Table 7.2). Only black-tailed
jackrabbits, Botta’s pocket gopher, and feral burros and
goats consume trunks and/or roots. Opuntia pads are a staple for only a few species (packrats, rabbits and jackrabbits,
javelina, Galápagos tortoise, Galápagos land iguanas, and
112
Mellink and Riojas-López
occasionally deer), although in some cases they only become a staple under drought conditions. Many of the consumers eat platyopuntias only sporadically.Tabe7l2.neahrere:
Cladode Preferences
Most studies reporting nopales in herbivore diets derive
their data from fecal analysis, which does not allow for
knowledge about differences in preference for different
cladodes of the plant. However, a difference in the palatability of young versus old cladodes would be expected.
Indeed, Berlandier’s tortoise prefers the younger, more tender cladodes that have fewer spines (Rose and Judd 1982).
In contrast, rabbits and black-tailed jackrabbits consume
the outer cladodes of Opuntia violacea var. macrocentra
TA B L E 7 . 2
Percentage of animals within each major taxon that consume different parts of platyopuntias
Percent
Taxon
Reptiles
Birds
Mammals
Overall
Number
Roots
Trunks
Pads
Petals
9
26
54
89
0
0
2
1
0
0
7
4
44
19
42
36
67
4
4
10
first, and then progress to the inside of the plants. This
process is more as a result of the architectural arrangement
of the plant, rather than due to differences in palatability
of the cladodes (Hoffman et al. 1993).
When different platyopuntia species are available in the
habitat, herbivores apparently prefer some over others. In
a test with three platyopuntia species and one cholla
species typical of nopaleras (nopal communities) in the San
Luis Potosí–Zacatecas Plateau, Mexico, white-throated
packrats prefer Opuntia robusta and O. streptacantha over
O. leucotricha and O. imbricata (Rangel and Mellink
1993). Opuntia robusta and O. streptacantha have the lowest amounts of protein and highest amounts of fiber
(Flores Valdez and Aguirre Rivera 1989), so the higher consumption by these packrats is not a response to nutritional quality. Rather, it apparently results from these cacti having fewer spines and glochids, which facilitates their
consumption. Collared peccaries prefer one of two different morphs of the same subspecies of O. phaeacantha,
again favoring the one with fewer spines (Theimer and
Bateman 1992). However, in New Mexico, spinescence apparently does not affect grazing intensity by lagomorphs;
rather, plant size and grazing history appear to be the factors that most determine grazing intensity; the proportion
of grazed pads increases for plants that have more than
seven cladodes (Hoffman et al. 1993).
Nopal growers and researchers indicate that some varieties of cultivated platyopuntias are more prone to consumption by wildlife than are others. So, ‘Copena F1,’ a
spineless forage nopal, is preferred by rodents and lagomorphs over other spineless varieties in central Mexico
(C. A. Flores-Valdés and F. Torres, personal communication). In some cases, lagomorphs can entirely wipe out a
commercial orchard of this variety. However, spines are not
a requirement for effective mechanical defense. Near
Jacumba, California, black-tailed jackrabbits will eat almost
anything except Opuntia basilaris (A. M. Rea, personal
Pollen and
nectar
22
35
2
13
Fruits
Seeds
67
69
61
64
0
38
15
20
communication). This species does not have large spines;
rather, it is densely covered with fine glochids (small, easily detached spines), a nasty encounter for herbivores.
To determine if white-throated packrats had foraging
preferences among different cultivated varieties of Opuntia
ficus-indica, rows of cultivars ‘Roja,’ ‘Amarilla,’ and ‘Blanca’
were examined at Las Papas de Arriba, Jalisco, Mexico.
Packrats clearly prefer to consume cladodes of ‘Roja’ over
‘Amarilla’ and prefer ‘Amarilla’ over ‘Blanca’ (Table 7.3).
Packrats build their dens on the ground against and around
the trunks of platyopuntias using various materials (e.g.,
cladodes, twigs, dung, garbage). As there are no differences
in the percentage of plants associated with packrat dens
among different varieties (Table 7.3), differences in consumption must be attributed to the forage quality of the
plants. The higher sugar content of ‘Roja’ presents one logical explanation for packrats’ preference.Tabe7l3n.eahrere:
Seasonal Effects for Herbivory
In xeric habitats where free water is scarce, cladodes become
an important source of water, when they are available. Not
unexpectedly, vertebrates increase their use of Opuntia
cladodes during the dry season, or use them only then. For
example, rabbits and black-tailed jackrabbits consume
Opuntia cladodes during the dry season, or when annuals
are scarce and other perennials have not developed new
growth (Hoffman et al. 1993). Berlandier’s tortoises consume more cladodes during the summer, as a source of
water (Auffenberg and Weaver 1969). Galápagos land iguanas reduce their preference for cladodes from about 32% of
bites in the dry season to 5% after the rains (Christian et al.
1984). Even finches, doves, and mockingbirds drink fluids
and eat moist pulp from cladodes in the Galápagos Islands
(Grant and Grant 1981). Other animals consume platyopuntias under special conditions; e.g., pronghorn antelope
feed readily on cladodes after a wildfire has burned off the
spines (Stelfox and Vriend 1977).
Consumption of Platyopuntias by Wild Vertebrates
113
TA B L E 7 . 3
Differences in feeding by packrats and evidence from packrat dens for adjacent rows of different
varieties of Opuntia ficus-indica cultivated in Las Papas de Arriba, Jalisco, Mexico
Nopal variety
‘Roja’
‘Amarilla’
Statistical significance
45
19
< 0.0001
8
12
n.s.
‘Amarilla’
‘Blanca’
Statistical significance
20
4
< 0.01
6
16
n.s.
Parameter
Platyopuntias with evidence of
feeding by packrats (% of plants)
Platyopuntias associated with
packrat dens (% of plants)
Platyopuntias with evidence of
feeding by packrats (% of plants)
Platyopuntias associated with
packrat dens (% of plants)
Unpublished observations of E. Mellink and M. E. Riojas-López.
The white-throated packrat is one of the vertebrates
that relies most heavily on platyopuntias for its food
(Vorhies and Taylor 1940; Spencer and Spencer 1941;
Rangel and Mellink 1993). Although Opuntia cladodes are
its preferred food, seasonal variations in preference occur.
In Arizona, this species exhibits clear peaks in consumption
of cladodes in May and November (Vorhies and Taylor
1940), which coincides with the driest period of the year.
The vertebrate most popularized as a cladode eater is
the collared peccary. Such fame is not undue, as platyopuntias are one of its most important food resources in arid
lands. Indeed, Opuntia cladodes are the most common
food for the peccary throughout the southwestern United
States and northern Mexico (Neal 1959; Leopold 1959;
Sowls 1984; Hoffmeister 1986). Peccaries are not ruminants, but their ruminant-like digestion allows them to use
profitably such a high-fiber forage (Langer 1979). Despite
their year-round high consumption of platyopuntias, collared peccaries exhibit seasonal variation, consuming them
in greater quantities during the summer (Corn and Warren
1985) or fall (Eddy 1961). In northern San Luis Potosi,
Mexico, collared peccaries steadily increase the amount of
cladodes in their diet from 25% in June to 66% in
September (Luévano et al. 1991). Cladode consumption
during the summer may be associated with high temperatures, and peccaries as well as white-tailed deer presumably
require the water for thermoregulation (Zervanos and
Hadley 1973). During drought, a collared peccary must
consume at least 2,300 g of succulent plants per day to
thermoregulate effectively (Langer 1979).
114
Mellink and Riojas-López
Deer are not always regarded as consumers of cladodes,
but in some arid regions they can rely heavily upon them,
at least during certain times of the year. In Big Bend
National Park, Texas, Opuntia engelmannii makes up 14%
of the yearly diet of mule deer and 10% of that of whitetailed deer (Krausman 1978). At other localities in Texas,
Opuntia cladodes form 30 to over 50% of the white-tailed
deer’s diet (Everitt and Gonzalez 1979; Quinton and
Horejsi 1977). Deer consume the cladodes both for energy and as a source of water for thermoregulation (Arnold
and Drawe 1979). Maximum cladode consumption by
deer has been reported to occur in the spring (Krausman
1978), summer (Arnold and Drawe 1979), and summer/fall
(Quinton and Horejsi 1977). Even within a given season,
great month-to-month variation occurs in the amount of
Opuntia cladodes consumed by deer (Luévano et al. 1991).
Differences in the timing of peak consumption reflect
water needs by the deer, along with the availability of free
water and other succulents that might provide water.
Less-studied animals also change their dependence on
cladodes during the year, depending on other available resources. For example, the northern pocket gopher consumes cladodes the entire year, but in dramatically higher
percentages during the winter, when the land is covered by
snow and little other food is available (Vaughan 1967).
Nutritional Qualities of Cladodes
Platyopuntia cladodes make a rather poor forage. Their
protein content varies between 3 and 13% (on a dry weight
basis), depending on the species, time of the year, whether
the plant bears fruit, the particular cladode, and the age of
the plant (Sowls 1984; Retamal et al. 1987; Flores Valdez
and Aguirre Rivera 1989; Pimienta-Barrios 1990; Gregory
and Felker 1992; Theimer and Bateman 1992). Moreover,
while some platyopuntias exhibit variations in protein and
phosphorus content associated with cladode age, others do
not (Gregory and Felker 1992). Still, at even the highest
level, the protein content is generally not sufficient for a
substantive diet. For example, when collared peccaries are
fed exclusively a cladode diet, they lose weight, but when
protein is supplemented, not only do they maintain their
weight, but some even gain weight (Sowls 1984). Collared
peccaries also demonstrated vitamin B deficiencies when
fed only cladodes (Sowls 1984). Regardless of its low nutritional value, the water provided by the cladodes is often
critical for the survival of consumers and appears to be
more important than any nutritional shortcomings at various times of the year. In any case, vertebrates seldom feed
only on platyopuntias, and the inclusion in their diet of
other plants with more protein, or invertebrates, prevent
them from severe undernutrition.
Not only are Opuntia cladodes a less than optimal food
for vertebrates, but there are also other risks associated with
their consumption. When collared peccaries are forced to
consume large quantities of nopales, large amounts of water
flow through the digestive system, causing an almost continuous diarrhea (Sowls 1984), which can have serious consequences in arid lands. Probably the best known negative
consequence of feeding on platyopuntias comes from the
high levels of the oxalates in them (Hodgkinson 1977;
Sowls 1984; Gibson and Nobel 1986). Dietary oxalates bind
calcium (Ca), magnesium (Mg), sodium (Na), and potassium (K) as highly insoluble compounds and, in sufficiently
high doses, commonly leads to hypocalcemia ( James 1972;
Hodgkinson 1977; Sowls 1984; Gibson and Nobel 1986).
The binding with Ca and Mg is likely the strongest (E.
Ezcurra, personal communication). Ill effects of high oxalic acid intake by mammals include nephritis and respiratory failure (Hodgkinson 1977; Sowls 1984). Although calcium oxalate crystals may cause mechanical damage to the
digestive system ( James 1972), those of Opuntia cladodes
are rather rounded, as opposed, for example, to crystals in
agaves, and may not be sufficiently abrasive to be a major
problem (E. Ezcurra, personal communication).
However, herbivores that have intestinal or ruminal
microflora capable of digesting cellulose can also degrade
calcium oxalate and absorb the calcium, if given enough
time for their digestive microbiota to adapt (Allison and
Cook 1981; Justice 1985). This appears to be the case with
packrats and other rodents (Shirley and Schmidt-Nielsen
1967; Justice 1985). This ability is provided by the adaptability of the digestive microflora itself, and does not represent an evolutionary adaptation of the herbivores ( Justice
1985). Herbivores cope with dietary oxalates in at least one
other way. Collared peccaries select inner cladodes that
have lower levels of oxalates (although lower spininess
seems to be also involved in this selection), but because
these cladodes also have less protein and more lignin, the
peccaries sacrifice diet quality (Theimer and Bateman
1992). Nopales also contain high quantities of alkaloids
(Meyer et al. 1980; Gibson and Nobel 1986). These substances could harm consumers of platyopuntias, but not
enough is known about them and their potential effects on
herbivores.
Spine Effects on Herbivory
Spines can potentially inflict wounds that can become infected (Anthony 1954). Several vertebrates, e.g., the collared peccary (Theimer and Bateman 1992) and whitethroated packrats (Rangel and Mellink 1993), feed less on
the more spiny cladodes. In the Galápagos Islands, spines
effectively prevent young arborescent platyopuntias from
being consumed by tortoises (Biggs 1990). However,
Berlandier’s tortoises feeding on cladodes are not hindered
much by spines, as several individuals with large spines in
the masseter muscles on both sides of the neck have been
observed (Auffenberg and Weaver 1969).
In addition to selecting cladodes that are less spiny, vertebrates often scrape the spines off. For example, collared
peccaries sometimes bite through the entire cladode, but
most commonly they step on it, peel the skin (epidermis
plus hypodermis) off one side, and then eat the pulp
(Sowls 1984). Captive Galápagos land iguanas fed cladodes
often scrape the surface with a front foot to remove the
spines before biting into the pad (Carpenter 1969). In
platyopuntia orchards in Jalisco, desert cottontails discard
the areoles and spines along with little pieces of cuticle
when feeding on cladodes. This explains the abundant
pieces of cuticle found at the bases of platyopuntias, together with cottontail rabbit fecal pellets. Platyopuntias
that have only jackrabbit pellets and no cuticle pieces suggest that the jackrabbits handle the spines in a different
manner. White-throated packrats, in addition to selecting
less spiny plants, most often gnaw across the cladode, beginning somewhere along its edge, and then work their
way inward, possibly discarding the spines and glochids
along the way (Fig. 7.1A). At other times they feed by
scraping the pulp from the side of the cladode (Fig. 7.1B).
In spite of the hazards that might be involved, the animals
that rely on Opuntia cladodes for an important part of
Consumption of Platyopuntias by Wild Vertebrates
115
A
B
Figure 7.1. Cladodes exhibiting signs of feeding by packrats near Las Papas, Jalisco, Mexico: (A) feeding on the edge and (B) feeding on the side.
116
their diet are able to cope with this resource, and death induced by such consumption in free-ranging conditions is
rather uncommon.Fgiure7n1.eahrere:
secondary consumers will include not only insects—ants,
bugs, wasps (Fig. 7.2) — but also birds and rodents
(Gonzalez-Espinoza and Quintana-Ascencio 1986).Fgiure72.neahrere:
Consumption of Reproductive Structures
Flower Consumption
The reproductive structures of platyopuntias, especially
fruits and seeds, are the major source of attraction to consumers. All the reptilian and bird consumers eat them, as
do most of the mammalian consumers (Table 7.1).
Actually, given a chance, all mammals that consume vegetative platyopuntia structures may readily eat flowers and
fruits as well. Flowers and fruit are available seasonally, but
the seeds can be available all year, because fruits ripen asynchronously, and because seeds can remain on the ground
for long periods.
Consumers of reproductive structures are of three foraging types: (1) primary foragers—those that directly reach
the flowers or fruits to feed on them; (2) secondary consumers—those that that feed on flowers or fruits once they
have been made available by a previous consumer; and (3)
tertiary consumers that feed on seeds, including those of
platyopuntias that are in the feces of other consumers, e.g.,
kangaroo rats, pocket mice, and canyon towhees, among
others (Gonzalez-Espinoza and Quintana-Ascencio 1986).
A primary consumer may open a fruit, allowing a secondary consumer to reach its interior, or it may cause petals,
fruits, or seeds to fall to the ground. Once a fruit has been
pried open by a primary consumer, such as a packrat, a
host of other users might eventually feed on it. These
Flowers provide more energy for herbivores than do the
cladodes or the fruits but only modest amounts of protein
(Christian et al. 1984). So the nutritive quality does not
seem to explain the “enthusiasm with which the iguanas
scramble for a newly fallen flower, nor does it demonstrate
the willingness of the iguanas to travel from tree to tree to
consume fallen flowers of Opuntia” (Christian et al. 1984).
Indeed, two-thirds of the reptiles that consume platyopuntias specifically eat the flowers (Table 7.2). Pollen and
nectar contained in the flowers might be part of the explanation for such a preference.
Some bird species on the Galápagos Islands consume
pollen and nectar during the dry season, switching to
fruits, seeds, and arthropods during the rainy season
(Grant and Grant 1979, 1981; Millington and Grant 1983).
During the dry season when other foods are scarce, the cactus finch on Daphne Major relies almost exclusively on
pollen and nectar, which seem to provide sufficient nutrients for pre-rain breeding. This is an advantage because
pairs of this finch that start breeding before the rains produce more offspring (Grant 1996). This finch is a true cactus specialist. To some extent, it excludes the other finches
from feeding on pollen and nectar of platyopuntias, defending larger territories year-round, which is in contrast
Mellink and Riojas-López
grains are valuable because they contain essential amino
acids and vitamins (Howell 1974; Grant and Grant 1981;
Millington and Grant 1983; Richardson et al. 1986; Grant
1996). Galápagos finches digest over 90% of the pollen
they consume (Grant 1996). The physiology of such high
digestibility has not been clarified, but either the Galápagos finches and lava lizards (which also exhibit a high digestion of pollen) are especially efficient, or platyopuntia
pollen is quite easy to digest (Grant 1996). Several paths are
theoretically possible in the digestion of pollen; for a small,
pollen-eating marsupial (Tarsipes rostratus), pollen seems to
be digested directly through the pores in its exine coat
(Richardson et al. 1986). Consuming nectar along with
pollen, in addition to providing energy, may also help the
ingested pollen germinate, facilitating its digestion (Grant
1996).
Fruit Consumption
Figure 7.2. Nopal fruit used as food source by a wasp after a primary
consumer has pried it open, near Las Papas, Jalisco, Mexico.
with other finches on Daphne Major, who defend smaller
territories and only for part of the year (Grant and Grant
1981; Grant 1996). Indeed, the ground finch on Daphne
Major consumes much lower amounts of nopal pollen and
nectar, and the onset of its breeding season is after the rains
(Grant 1996).
Doves on the Galápagos Islands feed on Opuntia
flowers, possibly first removing the stamens and nectar, and
then tearing and eating the petals. This seems to be a rather
unusual feeding habit—not often observed and not exhibited by all dove populations (Grant and Grant 1979). Lava
lizards on Pinta Island climb up platyopuntias and tear the
petals to obtain the pollen. In contrast, the lava lizard on
Daphne Major does not climb platyopuntias but obtains
pollen opportunistically by eating the pollen-impregnated
petals that fall when the Daphne Major cactus finch feeds
(East 1995). The lizards probably obtain an important portion of protein from the pollen, especially as the onset of
Opuntia flowering occurs during the dry season when
arthropods are in short supply (East 1995). Even the Lagarto
Tizón, an omnivorous lizard of the Canary Islands, Spain,
feeds on platyopuntia pollen (Valido and Nogales 1994).
Pollen has variable levels of protein. Notably, pollen
from bat-pollinated cactus flowers has a high protein content, e.g., up to 44% in the saguaro, but pollen of the nonbat-pollinated Opuntia versicolor has only 9% protein
(Howell 1974). In addition to their protein content, pollen
Opuntia fruits are a valuable food resource for animals and
are readily eaten when available. Over 60% of platyopuntia consumers in any taxa eat the fruits. Platyopuntia fruits
(tunas) have 9 to 18% sugar and large quantities of vitamin
C (Pimienta-Barrios 1990). Fruits of Opuntia lindheimeri
from southern Texas have 7% protein, 0.15% phosphorus
(P), 2.5% Ca, 0.93% Mg, 3.4% K, and 0.02% Na (Everitt
and Alaniz 1981). Vertebrates may consume fruits as a
“gourmet” food, when they encounter them, as the Shasta
ground sloth did (Hansen 1978). On the Canary Islands,
the endemic Lagarto Tizón consumes fruits of Opuntia dillenii during May (Valido and Nogales 1994). As different
platyopuntia species bear fruits of different sizes, color, and
spininess, foraging preferences of vertebrates differ ( Janzen
1986).
The seeds are used by at least 13 vertebrate herbivores,
mostly birds (Table 7.2). They are also an important resource for many rodents (González-Espinoza and
Quintana-Ascencio 1986). Such seeds are rich in oils and
proteins (Pimienta-Barrios 1990). Although seeds may be
available year-round, certain vertebrates eat them only
when other resources are in short supply. In the Galápagos
Islands during the dry season, seed consumption by the
Daphne Major cactus finch declines as flower feeding increases (Millington and Grant 1983).
Evolutionary and Ecological Context
Past and Present Herbivory Pressures
In arid and semiarid lands, platyopunitas often constitute
one of the most conspicuous elements of the landscape,
and it is easy to find relationships among them and some
Consumption of Platyopuntias by Wild Vertebrates
117
vertebrates. Platyopuntias provide protein, carbohydrates,
and water to vertebrates, and these vertebrates in turn act
as pollinators and dispersers (Grant and Grant 1979;
Gonzalez-Espinoza and Quintana-Ascencio 1986; Biggs
1990; East 1995). However, these relationships do not explain the evolution of the traits currently exhibited by
platyopuntias and their fruits. Rather, platyopuntias on
continental America are the ghosts of past interactions that
involve currently extinct megaherbivores ( Janzen and
Martin 1982; Gonzalez-Espinoza and Quintana-Ascencio
1986; Janzen 1986). On the Galápagos Islands, the evolutionary pressures, which are still operational, are different.
According to Janzen (1986), not only did Pleistocene
megaherbivores shape the form and anatomy of platyopuntias, but they also could have dispersed them from
South to North America, or vice versa, after the closure of
the Central American bridge. In addition to being longdistance dispersers in Pleistocene communities, some
megaherbivores probably munched their way through
dense patches of platyopuntias, creating clearings that
would be colonized by other plants and, perhaps, small
mammals, reptiles, and invertebrates. After most of the
megaherbivores of the Americas vanished at the end of the
Pleistocene, platyopuntias have maintained most of the
traits developed under the pressure of their former consumers. Certainly, important changes in distribution and
abundance resulted from the absence of their principal dispersers, but the species survived. In a few cases, erosion of
anachronistic traits (sensu Janzen and Martin 1982) seems
to be occurring; e.g., some platyopuntia species have
“spiny” fruits that are not eaten by herbivores and which
are mostly sterile (Anthony 1954).
Although they might have contributed little to the past
shaping of platyopuntias, the extant opuntiofagous vertebrates do currently serve as dispersers of seeds. Birds remove only modest amounts of seeds—less than 5% of the
total crop ( Janzen 1986)—but rodents can remove more
seeds from the fruits once they fall to the ground
(Gonzalez-Espinoza and Quintana-Ascencio 1986). Rodent
caches often become the source of new platyopuntias away
from the mother plants. The seed shadows produced by extant vertebrate dispersers are surely much different—and at
smaller geographical scales than from those that can be presumed for Pleistocene megaherbivores ( Janzen 1986).
Nonetheless, these extant vertebrates may substantially increase the cover of platyopuntia communties, especially
when patterns of competition among different plants is altered by the introduction of alien grazers, such as cattle or
sheep (Riegel 1941; Timmons 1942). The effects of direct removal of platyopuntia parts by extant species on the system
118
Mellink and Riojas-López
are difficult to assess. For example, collared peccaries can
remove 2 to 5% of the cladodes (Bissonette 1982). As platyopuntias are well armored against grazing, direct removal
of large parts of their vegetative structure is unlikely (and
probably was unlikely even during the Pleistocene), except
during severe drought.
Galápagos Islands
Nowhere is the association between platyopuntias and vertebrates as intense as on the Galápagos Islands. The morphology of nopales on different islands is a clear adjustment to avoid herbivory on vegetative structures
(Thornton 1971; Biggs 1990; Hicks and Mauchamp 1995).
Arborescent platyopuntias predominate on specific islands of the Galápagos that support, or have supported,
tortoise and land iguana populations. These plants have
large scaly trunks (> 60 cm in height), bearing rounded
compact crowns with lower cladodes strongly armored
with spines and the upper cladodes almost spineless.
When young, spines protect these nopales from grazing by
tortoises. On islands that have never supported tortoises or
iguanas, the plants are decumbent and have weak or no
spines, some cladodes bearing only tufts of glochids (Biggs
1990). During the rainy season, some cladodes of arborescent platyopuntias become heavy, turgid with water, and
break off from the mother plants, falling to the ground.
There they maintain a high water content, even through
the following dry season, when they are the main food
source for land iguanas and tortoises (Biggs 1990).
A particularly strong relationship exists between birds
and platyopuntias in the Galápagos Islands. In its simplest form, differences in size and hardness of platyopuntia seeds may be a partial response to predation by
finches. Conversely, the size and shape of the beaks of
finches may reflect an adjustment to forage efficiently for
pollen and nectar, as well as the ability to break seeds
(Grant and Grant 1989). Some finches contribute significantly to the cross-pollination of platyopuntias, transporting pollen from plant to plant. Such transport is important, as flowers that receive pollen from flowers of the
same plant produce significantly fewer seeds than those
that receive pollen from more distant plants (Grant and
Grant 1981). Consequently, these finches promote larger
seed crops.
However, this pollinating service is not without negative repercussions. When feeding on flowers, the finches
often snip off the styles, presumably to facilitate access to
the pollen, which prevents fertilization of the ovules. As a
result, by obtaining energy and protein from nectar and
pollen, the finches benefit by having an early onset of their
Figure 7.3. Feral burro eating the fruit of Opuntia aff. megacantha near Las Papas, Jalisco, Mexico.
breeding season, but, in doing so, threaten the seed supply
for their dry-season feeding (Grant and Grant 1981). The
negative effect of snipping styles might be only partial: the
later the style is snipped, the greater the chance that the
pollen has already reached the stigma and some ovules are
fertilized (Grant and Grant 1981). Despite their overall
value as pollinators, finches sometimes cause damage to
platyopuntia trunks and young cladodes when pecking
into them to drink fluid, eat storage tissues, and take insect
larvae (Grant and Grant 1981). As a result, the damaged
pads are vulnerable to infection by bacteria and fungi,
eventually leading to necrosis.
The preceeding is a highly simplified picture of the relationships that have shaped the Galapágos Islands platyopuntias and their communities. The real picture is more
complex and involves variations at different time scales.
Regrettably, the conditions that promoted the evolution of
the local relationships have not remained intact. The land
reptiles have been hunted, sometimes to extinction, and
alien species have been introduced (Thornton 1971; Hicks
and Mauchamp 1995). These events have already affected
the permanence of such relationships and probably will
cause others that might be detrimental to the conservation
of the entire platyopuntia-associated system.
Alienized Relations
Whenever platyopuntias have been introduced to other
parts of the world, they have been readily accepted by local
vertebrates. Ten animals have been reported to consume introduced platyopuntias (Leopard tortoise, Lagarto Tizón,
ostrich, emu, black magpie, little raven, baboon, “monkeys,” camel, and steenbok; Table 7.1), but there are probably many more unreported consumers. Frequently, native
vertebrates may become dispersers of alien platyopuntias,
which is the case for the Lagarto Tizón (Valido and
Nogales 1994) on the Canary Islands, the black magpie in
Australia (Darnell-Smith 1919), and primates and birds in
South Africa (Weed Section 1940). Other native vertebrates
that consume alien platyopuntia fruits may also disperse
seeds. Alien vertebrates may also disperse alien platyopuntias, as demostrated by feral European rabbits in Australia
(Darnell-Smith 1919). The roster of opuntiofagous vertebrates in areas were platyopuntias are alien is surely much
larger than what has been reported so far (Table 7.1), and
it is unlikely that it will ever be fully reported.
The other side of the coin in alienized relations is that
of the impact of alien vertebrates on native platyopuntias.
Four alien species currently consume cladodes in the wild
in the Americas (black rat; feral burro, Fig. 7.3; wild boar;
Consumption of Platyopuntias by Wild Vertebrates
119
and feral goat). When cattle and horses roamed wild two
centuries ago, they likely also engaged in cladode consumption, as domestic individuals of these species do
today. In continental America, the introduction of large domestic vertebrates (namely, cattle and horses) restored functions interrupted by the extinction of megafauna at the end
of the Pleistocene (Martin 1975; Janzen 1986), although this
view is not always accepted. In insular contexts, however,
the introduction of alien herbivores or omnivores often
causes conservation hazards, if not mass extinction, even for
well-protected species, such as platyopuntias.Fgiure73n.eahrere:
Three alien vertebrates threaten platyopuntias on the
Galápagos Islands. Goats feed on the pads of nopal (Hicks
and Mauchamp 1995). They munch through the trunks of
arboreal platyopuntias, up to 50 cm in diameter, causing
them to fall (Eliasson 1968). Medium and large platyopuntias are subject to a higher grazing pressure, because
they are less spiny, and this selection can seriously impair
platyopuntia populations, as it leads to the killing of the
plants before they reach reproductive age (Hicks and
Mauchamp 1995). Fallen cladodes can produce new trees,
but the goats eagerly eat the pads before any rooting takes
place (Hicks and Mauchamp 1995). Burros have also
affected the distribution of platyopuntias on the Galápagos
Islands (Van der Werff 1982; Hicks and Mauchamp 1995).
In the case of seedlings, their heavy spiny armour can prevent grazing by native reptiles but does not prevent goats
from considering them a “favorite” (Schofield 1989). While
not evident at first consideration, mice (probably Mus musculus) are also a threat to platyopuntias on the Galápagos
Islands (Snell et al. 1994). They burrow among and into the
roots, weakening their hold on the soil. The effect of such
activities and the success of prevention programs in the
long run is difficult to predict.
Concluding Remarks
Platyopuntia cladodes offer not only food and water, but
also protective cover, den anchorage, and den building materials to wild vertebrates. Several species are closely associated with platyopuntias: Berlandier’s tortoise, whitethroated packrat, collared peccary, and deer, as well as
finches, land iguanas, and tortoises on the Galápagos
Islands. Other vertebrates rely on cladodes for survival during critical periods such as drought. Still other species, although not using the cladodes themselves, use the native
platyopuntia nopaleras as habitat.
Despite the fact that several relationships between
platyopuntias and vertebrates exist, there has been a
paucity in the efforts to understand them, except for the
highly creative studies on the Galápagos Islands. For the
120
Mellink and Riojas-López
continental Americas, the advances in understanding
platyopuntia-vertebrate interactions notably include the
proposal of Janzen (1986) on the evolution of platyopuntias, their communities, and their dispersal in North
America as well as the work of González-Espinoza and
Quintana-Ascencio (1986) on Opuntia seed dispersal for
nopaleras in the Mexican plateau. Most other work has
focused on the role of cladodes in the diet of selected vertebrates, especially the collared peccary. Clearly, much research remains to be done to understand the function of
nopaleras in the continental Americas. If such an understanding is to be generated, action should be taken soon,
as nopaleras are being modified at accelerated rates to raise
agricultural products or livestock, or as an inevitable side
effect of human population growth.
Acknowledgments
We thank Rosy Licón, Roberto García-Benitez, Dolores
Sarracino, and Park Nobel, who helped obtain some of the
bibliographic sources, and Amadeo Rea and Exequiel
Ezcurra for insightful reviews.
Literature Cited
Allison, M. J., and H. M. Cook. 1981. Oxalate degradation
by microbes of large bowel of herbivores: The effect of
dietary oxalate. Science 212: 675–676.
Anthony, M. 1954. Ecology of the Opuntiae in the Big
Bend region of Texas. Ecology 35: 334–347.
Arnold, L. A., Jr., and D. L. Drawe. 1979. Seasonal food
habits of white-tailed deer in the south Texas plains.
Journal of Range Management 32: 175–176.
Auffenberg, W., and W. G. Weaver. 1969. Gopherus
berlandieri in southern Texas. Bulletin of the Florida
State Museum 13: 141–203.
Baber, D. W., and B. E. Coblentz. 1987. Diet, nutrition,
and conception in feral pigs on Santa Catalina Island.
Journal of Wildlife Management 51: 306–317.
Bailey, V. 1931. Mammals of New Mexico. North American
Fauna 53: 1–412.
Biggs, A. L. 1990. Coevolution in the Galápagos. American
Biology Teacher 52: 24–28.
Bissonette, J. A. 1982. Ecology and social behavior of the
collared peccary in Big Bend National Park. National
Park Service Scientific Monograph 16: 1–85.
Blair, W. F. 1937. The burrows and food of the prairie pocket mouse. Journal of Mammalogy 18: 188–191.
Bowman, R. I. 1961. Morphological differentiation and
adaptation in the Galápagos finches. University of
California Publications in Zoology 58: 1–302.
Brown, D. E. 1989. Arizona Game Birds. University of
Arizona Press, Tucson.
Burt-Davy, J. 1920. Utilizing prickly pear and spineless cactus. South African Journal of Industry 3: 1001–1011.
Carpenter, C. C. 1969. Behavioral and ecological notes on
the Galápagos land iguanas. Herpetologica 25: 155–164.
Christian, K. A., C. R. Tracy, and W. P. Porter. 1984. Diet,
digestion, and food preferences of Galápagos land
iguanas. Herpetologica 40: 205–212.
Clements, F. E., and V. E. Shelford. 1939. Bio-Ecology.
Wiley, New York.
Corn, J. L., and R. J. Warren. 1985. Seasonal habits of the
collared peccary in south Texas. Journal of Mammalogy
66: 155–159.
Dalquest, W. W. 1953. Mammals of the Mexican state of
San Luis Potosí. Lousiana State University Studies,
Biological Sciences Series 1: 1–112.
Darnell-Smith, G. P. 1919. Animal aids to the spread of
prickly pear. Agricultural Gazette of New South Wales
30: 125–127.
Davis, O. K., L. Agenboard, P. S. Martin, and J. I. Mead.
1984. The Pleistocene blanket of Bechan Cave, Utah.
Special Publication of Carnegie Museum of Natural
History 8: 267–282.
Dodd, N. L., and W. W. Brady. 1988. Dietary relationships
of sympatric desert bighorn sheep and cattle. Desert
Bighorn Council Transactions 1988: 1–6.
Dunn, J. P., J. A. Chapman, and R. E. Marsh. 1982.
Jackrabbits. In Wild Mammals of North America ( J. A.
Chapman and G. A. Feldhamer, eds.). Johns Hopkins
University Press, Baltimore, Maryland. Pp. 124–145.
East, K. T. 1995. Pollen digestion in Galápagos lava lizards.
Noticias de Galápagos 55: 8–14.
Eddy, T. A. 1961. Foods and feeding patterns of the collared
peccary in southern Arizona. Journal of Wildlife Management 25: 248–257.
Eliasson, U. 1968. On the influence of introduced animals
on the natural vegetation of the Galápagos Islands.
Noticias de Galápagos 11: 19–21.
Everitt, J. H., and M. A. Alaniz. 1981. Nutrient content of
cactus and woody plant fruits eaten by birds and mammals in south Texas. Southwestern Naturalist 26: 301–
305.
Everitt, J. H., and C. L. Gonzalez. 1979. Botanical com-
position and nutrient content of fall and early winter
diets of white-tailed deer in south Texas. Southwestern
Naturalist 24: 297–310.
Everitt, J. H., C. L. Gonzalez, M. A. Alaniz, and G. V.
Latigo. 1981. Food habits of the collared peccary on
south Texas rangelands. Journal of Range Management
34: 141–144.
Flores Valdez, C. A., and J. R. Aguirre Rivera. 1989. El
Nopal como Forraje, 2nd ed. Universidad Autónoma
Chapingo, Chapingo, Mexico.
Gibson, A. C., and P. S. Nobel. 1986. The Cactus Primer.
Harvard University Press, Cambridge, Massachusetts.
Gonzalez-Espinoza, M., and P. F. Quintana-Ascencio.
1986. Seed predation and dispersal in a dominant
desert plant: Opuntia, ants, birds, and mammals. In
Frugivores and Seed Dispersal (A. Estrada and T. H.
Fleming, eds.). W. Junk, Dordrecht, The Netherlands.
Pp. 273–284.
Grant, B. R. 1996. Pollen digestion by Darwin’s finches
and its importance for early breeding. Ecology 77: 489–
499.
Grant, B. R., and P. R. Grant. 1981. Exploitation of
Opuntia cactus by birds on the Galápagos. Oecologia
49: 179–187.
Grant, B. R., and P. R. Grant. 1989. Natural selection in a
population of Darwin’s finches. American Naturalist
133: 377–393.
Grant, P. R., and K. T. Grant. 1979. Breeding and feeding
ecology of the Galápagos dove. Condor 81: 397–403.
Gregory, R. A., and P. Felker. 1992. Crude protein and
phosphorus contents of eight contrasting Opuntia
clones. Journal of Arid Environments 22: 323–331.
Hansen, R. M. 1978. Shasta ground sloth food habits,
Rampart Cave, Arizona. Paleobiology 4: 302–319.
Hellgren, E. C. 1993. Status, distribution, and summer
food habits of black bears in Big Bend National Park.
Southwestern Naturalist 38: 77–80.
Hicks, D. J., and A. Mauchamp. 1995. Size-dependent predation by feral mammals on Galápagos Opuntia.
Noticias de Galápagos 55: 6–17.
Hodgkinson, A. 1977. Oxalic Acid in Biology and Medicine.
Academic Press, New York.
Hoffman, M. T., J. D. James, G. I. H. Kerley, and W. G.
Whitford. 1993. Rabbit herbivory and its effects on
cladode, flower and fruit production of Opuntia violacea var. macrocentra (Cactaceae) in the northern Chi-
Consumption of Platyopuntias by Wild Vertebrates
121
huahuan Desert, New Mexico. Southwestern Naturalist
38: 309–315.
Marais, J. G. 1939. Spineless cactus and old-man saltbush
for sheep. Farming in South Africa 1939: 99–100.
Hoffmeister, D. F. 1986. Mammals of Arizona. University
of Arizona Press, Tucson.
Martin, P. S. 1975. Vanishings, and future, of the prairie.
Geoscience and Man 10: 39–49.
Howell, D. J. 1974. Bats and pollen: Physiological aspects
of syndrome of chiropterophyly. Comparative Biochemistry and Physiology, Series A 48: 263–276.
Martinez-Romero, L. E., and S. Mandujano. 1995.
Habitats alimentarios del pecarí de collar (Pecari tajacu) en un bosque tropical caducifolio de Jalisco,
Mexico. Acta Zoologica Mexicana 64: 1–20.
James, L. F. 1972. Oxalate toxicosis. Clinical Toxicology 5:
231–243.
Janzen, D. H. 1986. Chihuahuan Desert nopaleras:
Defaunated big mammal vegetation. Annual Review of
Ecology and Systematics 17: 595–636.
Janzen, D. H., and P. S. Martin. 1982. Neotropical
anachronisms: The fruits the gomphotheres ate. Science
215: 19–27.
Justice, K. E. 1985. Oxalate digestibility in Neotoma albigula and Neotoma mexicana. Oecologia 67: 231–234.
Krausman, P. R. 1978. Forage relationships between two
deer species in Big Bend National Park, Texas. Journal
of Wildlife Management 42: 101–107.
McCulloch, E. M. 1980. Little raven Corvus mellori eating
fruit of prickly pear Opuntia sp. Australian Bird
Watcher 8: 248.
McLure, M. F., N. S. Smith, and W. W. Shaw. 1995. Diets
of coyotes near the boundary of Saguaro National
Monument and Tucson, Arizona. Southwestern
Naturalist 40: 101–105.
Meyer, B. N., A. H. Mohamed, and J. L. MacLaughlin.
1980. β-phenethylamines from the cactus genus
Opuntia. Phytochemistry 19: 719–720.
Krausman, P. R., B. D. Leopold, R. F. Seegmiller, and
S. G. Torres. 1989. Relationship between desert
bighorn sheep and habitat in western Arizona. Wildlife
Monographs 102: 1–66.
Mijares-Urrutia, A., B. Colvée, and A. Arend R. 1997.
Sauria: Cnemidophorus lemniscatus (NCN). Herbivory.
Herpetological Review 28: 88.
Lack, D. 1947. Darwin’s Finches. Cambridge University
Press, Cambridge, England.
Millington, S. J., and P. R. Grant. 1983. Feeding ecology
and territoriality of the cactus finch Geospiza scandens
on Isla Daphne major, Galapagos. Oecologia 58: 76–83.
Langer, P. 1979. Adaptational significance of the fore
stomach of the collared peccary, Dicotyles tajacu (L.
1758) (Mammalia: Artiodactyla). Mammalia 43: 235–
245.
Lehman, V. W. 1984. Bobwhites in the Rio Grande Plains of
Texas. Texas A&M University Press, College Station,
Texas.
Leopold, A. S. 1959. Wildlife of Mexico. University of
California Press, Berkeley.
Long, A., R. M. Hansen, and P. S. Martin. 1974. Extinction of the shasta ground sloth. Geological Society of
America Bulletin 85: 1843–1848.
Luévano E. J., E. Mellink, E. García M., and R. Aguirre R.
1991. Dieta y traslapo dietario del venado cola blanca,
jabalí de collar, cabra y caballo, durante el verano en la
Sierra de la Mojonera, Vanegas, S.L.P. Agrociencia, serie
Recursos Naturales Renovables 1: 105–122.
MacCracken, J. G., and R. M. Hansen. 1984. Seasonal
foods of blacktail jackrabbits and Nuttall cottontails in
southeastern Idaho. Journal of Range Management 37:
256–259.
122
McClinton, S. F., P. L. McClinton, and J. V. Richerson.
1992. Food habits of black bears in Big Bend National
Park. Southwestern Naturalist 37: 433–435.
Mellink and Riojas-López
Milton, S. J. 1992. Plants eaten and dispersed by adult
leopard tortoises Geochelone pardalis (Reptilia:
Chelonii) in the southern Karoo. South African Journal
of Zoology 27: 45–49.
Neal, B. J. 1959. A contribution on the life history of the
collared peccary in Arizona. American Midland
Naturalist 61: 177–190.
Pimienta-Barrios, E. 1990. El Nopal Tunero. Universidad de
Guadalajara, Zapopan, Mexico.
Price, T. 1987. Diet variation in a population of Darwin’s
finches. Ecology 68: 1015–1028.
Quinton, D. A., and R. G. Horejsi. 1977. Diets of whitetailed deer on the rolling plains of Texas. Southwestern
Naturalist 22: 505–509.
Quinton, D. A., R. G. Horejsi, and J. T. Flinders. 1979.
Influence of brush control on white-tailed deer diets in
north-central Texas. Journal of Range Management 32:
93–97.
Rangel, M. G., and E. Mellink. 1993. Historia natural de
la rata magueyera en el Altiplano Mexicano. In Avances
en el Estudio de los Mamíferos de México (R. A.
Medellín and G. Ceballos, eds.). Asociación Mexicana
de Mastozoología, Mexico City. Pp. 173–183.
Retamal, N., J. M. Duran, and J. Fernandez. 1987.
Seasonal variation of chemical composition in prickly
pear (Opuntia ficus-indica [L.] Miller). Journal of the
Science of Food and Agriculture 38: 303–311.
Richardson, K. C., R. D. Wooler, and B. G. Collins. 1986.
Adaptations to a diet of nectar and pollen in the marsupial Tarsipes rostratus (Marsupialia: Tarsipedidae).
Journal of Zoology 208: 285–297.
white-throated wood rat in Arizona. Journal of
Mammalogy 22: 280–284.
Stelfox, J. G., and H. G. Vriend. 1977. Prairie fires and
pronghorn use of cactus. Canadian Field Naturalist 91:
282–285.
Theimer, T. C., and G. C. Bateman. 1992. Patterns of
prickly-pear herbivory by collared peccaries. Journal of
Wildlife Management 56: 234–240.
Thornton, I. 1971. Darwin´s Islands; a Natural History of
the Galápagos. American Museum of Natural History,
Garden City, New York.
Riegel, A. 1941. Some coactions of rabbits and rodents with
cactus. Transactions of the Kansas Academy of Sciences
44: 96–101.
Timmons, F. L. 1942. The dissemination of prickly pear
seed by jack rabbits. Journal of the American Society of
Agronomy 34: 513–520.
Rose, F. L., and F. W. Judd. 1982. Biology and status of
Berlandier’s tortoise (Gopherus berlandieri). In North
American Tortoises: Conservation and Ecology (R. B.
Bury, ed.). Wildlife Resarch Report 12. United States
Fish and Wildlife Service, Washington, D.C. Pp. 57–
70.
Valido, A., and M. Nogales. 1994. Frugivory and seed dispersal by the lizard Gallotia galloti (Lacertidae) in a
xeric habitat of the Canary Islands. Oikos 70: 403–411.
Russell, P. T. 1964. Antelope of New Mexico. New Mexico
Department of Fish and Game, Santa Fe.
Schluter, D. 1984. Body size, prey size and herbivory in the
Galápagos lava lizard, Tropidurus. Oikos 43: 291–300.
Schofield, E. K. 1989. Effects of introduced plants and animals on island vegetation: Examples from the
Galápagos Archipelago. Conservation Biology 3: 227–
238.
Sexson, M. L., J. R. Choate, and R. A. Nicholson. 1981.
Diet of pronghorn in western Kansas. Journal of Range
Management 34: 489–493.
Shirley, E. K., and K. Schmidt-Nielsen. 1967. Oxalate metabolism in the pack rat, sand rat, hamster, and white
rat. Journal of Nutrition 91: 496–502.
Snell, H. L., H. M. Snell, and P. Stone. 1994. Accelerated
mortality of Opuntia on Isla Plaza Sur: Another threat
from an introduced vertebrate? Noticias de Galápagos
53: 19–21.
Sowls, L. K. 1984. The Peccaries. University of Arizona
Press, Tucson.
Spaulding, W. G., and P. S. Martin. 1979. Ground sloth
dung of the Guadalupe mountains. National Park
Service Proceedings 4:259.269.
Spencer, D. A., and A. L. Spencer. 1941. Food habits of the
Van der Werff, H. 1982. Effects of feral pigs and donkeys
on the distribution of selected food plants. Noticias de
Galápagos 36: 17–18.
Vaughan, T. A. 1967. Food habits of the northern pocket
gopher on shortgrass prairie. American Midland
Naturalist 77: 176–189.
Vorhies, C. T., and W. P. Taylor. 1922. Life history of the
kangaroo rat Dipodomys spectabilis spectabilis Merriam.
Bulletin of the United States Department of Agriculture
1091: 1–40.
Vorhies, C. T., and W. P. Taylor. 1933. The life histories and
ecology of jack rabbits, Lepus alleni and Lepus californicus ssp., in relation to grazing in Arizona. University
of Arizona College of Agriculture Technical Bulletin 49:
471–587.
Vorhies, C. T., and W. P. Taylor. 1940. Life history and
ecology of the white-throated wood rat, Neotoma albigula albigula Hartley, in relation to grazing in
Arizona. University of Arizona College of Agriculture
Technical Bulletin 86: 455–529.
Weed Section [South African] Department of Agriculture
and Forestry. 1940. Opuntias in South Africa. I: Some
naturalized species with special reference to prickly
pear, and methods for its eradication. Farming in
South Africa 15: 119–125.
Zervanos, S. M., and N. F. Hadley. 1973. Adaptational biology and energy relationships of the collared peccary
(Tayassu tajacu). Ecology 54: 759–774.
Consumption of Platyopuntias by Wild Vertebrates
123
CHAPTER
›8‹
B I O D I V E R S I T Y A N D C O N S E RVAT I O N
Thomas H. Boyle and Edward F. Anderson
Introduction
Biodiversity of Cacti
Biodiversity of Natural Communities of Cacti
Genetic Diversity in Wild Cacti
Breeding Systems and Sexual Expression in the Cactaceae
Biodiversity of Cultivated Cacti
Conservation of Cacti
Why Cacti Are Threatened
Determination of Threatened Cacti
Conservation of Specific Cacti
Importance of Artificial Propagation
CITES
The Convention on Biological Diversity
In situ and ex situ Conservation
Conclusions and Future Prospects
Literature Cited
Introduction
Cacti have been exploited since pre-Columbian times.
Many cacti have fruits that are eaten raw, cooked, or fermented into alcoholic beverages. Stems of some cacti are
cooked as a vegetable, used as an emergency source of
water, or fed to domestic animals as forage or fodder. Dried
woody stems of some large columnar cacti are gathered for
building material or firewood. Some species produce substances of pharmaceutical or industrial interest (e.g.,
cochineal dye extracted from the dried bodies of a scale insect [Dactylopius coccus] collected from many species of
prickly-pear cacti), whereas other cacti are consumed to induce visual hallucinations for religious or recreational
purposes. Last but not least, cacti are prized for their diverse forms and beautiful flowers and are cultivated worldwide as ornamentals. Unfortunately, habitat destruction
and collection of wild cacti has threatened the very survival
of some species, and attention now must be focused on
their protection.
The conservation of biodiversity is one of the major issues facing humankind today and is of paramount importance for the long-term survival of cacti as well as other organisms. This chapter examines the biodiversity of both
wild and cultivated cacti, and discusses contemporary and
long-term issues pertaining to conservation of cacti.
Factors affecting biodiversity of cacti and the estimation of
genetic diversity in wild and cultivated cacti are considered
125
first. Then the effects of habitat destruction and collection
on biodiversity loss of wild cacti are discussed and international activities that encourage the conservation of cacti
are presented.
Biodiversity of Cacti
The term biodiversity refers to the range of variation or
differences exhibited by biological organisms and their environments (Barbier 1997). Biodiversity is essential for the
adaptation to specific environments and for the continuing evolution of species. It may refer to variation present
within a gene, an organism, a species (with extinction representing the total and irreversible loss of biological diversity), a population, or an ecosystem (or community).
Scientists, conservationists, and policymakers are interested in several aspects of biodiversity, including the maintenance of biodiversity, the impact of biodiversity loss, and
the establishment of priorities for biodiversity conservation. However, a single biodiversity indicator does not
apply to all conservation issues. Reid et al. (1993) listed 22
indicators relating to different conservation issues and
grouped them into three categories: (1) those used for estimating diversity within wild species; (2) those used for estimating diversity in domesticated plants and animals, e.g.,
crops or livestock; and (3) those used for estimating diversity within communities or habitats. For this review, biodiversity of cacti will be examined in each of these three
conservation systems.
Biodiversity of Natural Communities of Cacti
Cacti inhabit a wide diversity of climatic regions and
ecosystems throughout much of the New World. Their
range extends from 58oN latitude in Canada (Opuntia fragilis) to 50oS in Patagonia (Maihuenia, Opuntia, Pterocactus); in South America, from the islands of Fernando de
Noronha off Brazil’s Atlantic coast to the Galápagos
Archipelago in the Pacific; and, in North America, from
the coast of Massachusetts (Opuntia humifusa) to southern
Vancouver Island (O. fragilis; Barthlott and Hunt 1993).
Their altitudinal distribution varies from coastal habitats
throughout much of North and South America to about
4,500 m in the Andean uplands of Peru and Chile (Austrocylindropuntia floccosa, A. lagopus; formerly Opuntia
floccosa and O. lagopus; Barthlott and Hunt 1993). Cacti are
most plentiful in the arid and semiarid regions between
35oN and 35oS (Taylor 1997), but about 10% of all species
are epiphytic or epilithic and are found in more humid regions (Barthlott 1983). Species of cacti are sparse toward the
periphery of the family’s range and in its equatorial center
but relatively abundant in four geographical regions, or
126
Boyle and Anderson
TA B L E 8 . 1
Centers of diversity and endemism for the Cactaceae
Geographic region
Mexico; southwestern United States
Central Andes of Peru and Bolivia,
southern Ecuador, northeastern
Chile, northwestern Argentina
Eastern Brazil
Central western and southern Brazil,
Paraguay, Uruguay, southern and
eastern Argentina
Endemic
genera
Species
present
28
19
570
535
12
—
145
85
Data are from Taylor (1997) for the approximately 125 genera in the
Cactaceae and for species on the CITES Cactaceae checklist (Hunt
1992).
centers of diversity (Table 8.1). Collectively, these four centers of diversity contain approximately half of all known
species of cacti and substantial numbers of endemics. Two
other regions, i.e., the Caribbean basin (including northern Venezuela, Panama, and northern and western Colombia) and Chile (excluding the northeastern corner [see
Table 8.1]), also contain numerous endemics.Tabe8ln1.eahrere:
A primary objective of conservation is to maintain
species diversity within a defined locale or region. In most
cases, species diversity has been interpreted as “species richness,” i.e., the quantity of species within a country, ecosystem, or region. For cacti, as for other species, species diversity is greatest in the centers of diversity; e.g., one locale in
northeast Mexico has 27 species of cacti (Taylor 1997). An
emphasis on preserving plant communities with the greatest species richness has meant that the major focus of conservation measures in the Americas has been on tropical
moist broadleaf forests (“rainforests”) rather than the drier,
nonforested or semiforested regions (Redford et al. 1990).
However, this latter group of ecosystems contains the vast
majority of cacti. Hence, many of the conservation measures designed to preserve tropical rainforests in the
Americas have had little impact on conservation of cacti.
Genetic Diversity in Wild Cacti
Wild species of plants experience numerous changes in
their biological and physical environment over time. Their
survival, evolutionary potential, and ability to adapt to environmental changes will depend on the existence of genetic diversity. As a consequence, considerable effort has
been made to estimate the genetic diversity within wild
species of plants. Information on genetic diversity in wild
species has come primarily from allozyme surveys. Allozymes exhibit simple inheritance, codominance, complete
penetrance, and consistency of expression under a wide
range of environmental conditions and can be determined for a wide range of plant species irrespective of natural habitat, size, or longevity (Hamrick 1989). These
properties make allozymes particularly useful as genetic
markers.
Allozyme surveys estimate the level of genetic diversity within individual species and indicate how the genetic
variation is distributed within and among populations. A
typical allozyme survey consists of three parts: (1) collection
of allozyme data from a minimum of one population (usually more) of a species, (2) computation of diversity statistics, and (3) comparison of the species’ diversity statistics
with those collected from other species with similar life history traits (Hamrick and Godt 1989, 1996). The following
statistics are calculated at the species and within-population levels: (1) percentage of polymorphic loci (P), (2)
mean number of alleles per locus (A) for polymorphic as
well as monomorphic loci, and (3) genetic diversity (H) of
each locus (H = 1 –∑pi2, where pi is the mean frequency of
the ith allele at a locus). Mean genetic diversity at the
species and within-population levels is determined by averaging the H values over all loci. For the average plant
species, P, A, and H values are 50%, 1.96, and 15%, respectively (Hamrick and Godt 1989). The P, A, and H values
for the average plant population are 34%, 1.53, and 11%, respectively. For studies that examine allozyme variation in
multiple populations, the genetic diversity within populations (HS) and the total genetic diversity (HT) are computed for polymorphic loci, and the GST statistic is calculated to estimate the proportion of total diversity among
populations (GST = [HT –HS]/HT [Nei 1973]). For the average plant species, about 78% of the total genetic diversity (HT) at polymorphic loci resides within populations,
whereas 22% of the HT is distributed among populations
(Hamrick and Godt 1989).
Allozyme surveys have been conducted on natural populations of Opuntia humifusa (Wallace and Fairbrothers
1986), Pachycereus schottii (Parker and Hamrick 1992), and
Weberbauerocereus weberbaueri (Sahley 1996). The most
thorough genetic diversity study on wild cacti was on the
diploid (2n = 22) species P. schottii (formerly Lophocereus
schottii; Parker and Hamrick 1992). Eight populations of P.
schottii were examined at the northern extremity of its
range (southern Arizona), where reproduction is primarily asexual. At the species level, the percent polymorphic
loci was 44%, the mean number of alleles per locus was
1.55, and the mean genetic diversity (He) was 0.145. The P
value for P. schottii (44%) was comparable to the mean P
value reported by Hamrick and Godt (1989) for dicots
(45%), species with a narrow distribution range (45%), and
species that reproduce sexually and asexually (44%). The
mean proportion of polymorphic loci and mean genetic diversity (He) within populations were 34% and 0.126. The
mean proportion of total diversity among populations
(GST) was 0.13. Thus, about 87% of the total genetic variation occurred within populations. The mean GST value
for P. schottii was lower than for groups of species with similar ecological traits—dicots, species with a narrow distribution range, species that disperse seed by animal ingestion, and species that reproduce sexually and asexually
(Hamrick and Godt 1989). Sporadic long-distance dispersal of stems and occasional sexual reproduction apparently helped sustain a level of genetic diversity in P. schottii
that is similar to sexually reproducing species. Previous
studies (Ellstrand and Roose 1987; Hamrick and Godt
1989) have shown that species that reproduce primarily or
exclusively by asexual means (vegetative reproduction
and/or agamospermy) maintain as much genetic diversity
as species that reproduce sexually.
Information about the extent and distribution of genetic variation in plant species has both scientific merit and
practical applications. For example, allozyme data can be
useful for determining how to collect and maintain genetically representative samples for conserving genetic diversity in ex situ collections. Information on the distribution
of genetic variation within and among populations has
aided in the development of sampling strategies for collecting plant materials of endangered species (Brown and
Briggs 1991).
Breeding Systems and Sexual Expression in the Cactaceae
The breeding system markedly affects the magnitude and
distribution of genetic diversity in plant species. The
Cactaceae can be roughly divided into two groups, depending on whether they are predominantly outcrossing or
primarily selfing. Most cacti probably outcross to some degree, and many species are likely to be predominantly outcrossing. Cacti exhibit several mechanisms that encourage
outcrossing, including self-incompatibility, herkogamy,
and dicliny. Self-incompatibility is a genetically controlled
mechanism that promotes outcrossing in fertile hermaphrodites by preventing fertilization when a plant is selfed or
outcrossed to another plant with the same incompatibility phenotype (de Nettancourt 1977). It is widespread in the
Cactaceae and has been documented in at least 30 of its approximately 120 genera (Boyle 1997). These genera comprise all three of the traditional Cactaceae subfamilies
Biodiversity and Conservation
127
(Pereskioideae, Opuntioideae, and Cactoideae) and eight
of the nine Cactoideae tribes. Self-incompatibility can
minimize inbreeding, but, as pointed out by Olmstead
(1986), the extent of inbreeding within a population depends primarily on population size rather than on the type
of breeding system. Genetic diversity is rapidly lost from
small populations due to inbreeding and genetic drift (random changes in allelic frequencies; Wilcox 1984).
Dicliny occurs when some members of a population
normally produce flowers that are unisexual instead of hermaphroditic and has been reported in several Cactaceae
taxa. Dioecy (co-occurrence of androecious and gynoecious plants) occurs in Echinocereus coccineus (Hoffmann
1992) and Opuntia stenopetala (Parfitt 1985). Gynodioecy
(consisting of hermaphroditic and gynoecious plants) has
been reported in Mammillaria dioica (Ganders and
Kennedy 1978; Parfitt 1985) and M. neopalmeri (Parfitt
1985). Trioecy (co-occurrence of androecious, gynoecious,
and hermaphroditic plants) occurs in P. pringlei (Fleming
et al. 1994) and Selenicereus innesii (Innes and Glass 1991).
Hermaphroditic, dioecious, and trioecious populations
have been documented for Opuntia robusta (Parfitt 1985;
del Castillo 1986; Hoffmann 1992). Each type of dicliny
promotes outcrossing but to different degrees. Dioecy ensures 100% outcrossing. With gynodioecy and trioecy, the
level of outcrossing depends on the frequency of females in
the population, degree of selfing in hermaphrodites, and
pollinator activity. Murawski et al. (1994) used allozyme
analysis to examine the mating system in P. pringlei (a selfcompatible trioecious species); the estimated proportion of
outcrossing in females was nearly 1.0 (0.949) but the proportion was markedly lower (0.30) in hermaphroditic individuals, indicating that the majority of seeds produced
by hermaphrodites result from selfing.
Pollen must be transferred between plants with dissimilar incompatibility phenotypes or dissimilar floral
morphologies (diclinous taxa) for seed to set. As far as is
known, cacti are pollinated exclusively by animals (Porsch
1938, 1939; Rowley 1980; Grant and Grant 1979; Grant and
Hurd 1979; Schlindwein and Wittmann 1997). Animals
also serve as dispersal agents for fruit and seed of cacti
(Gates 1932; Wallace and Fairbrothers 1986). Hence, cacti
and their animal pollinators/dispersers have developed mutualistic relationships, with animals receiving nectar and/or
pollen as a reward for pollination or, in the case of
seed/fruit dispersal, receiving nutrients from the fruit
pulp or digested seeds. The incapacity to set seed due to
absence of pollinators has profound effects on the genetic
structure, mating system, and selection forces of obligately outcrossed species (Olesen and Jain 1994). Any distur-
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Boyle and Anderson
bance to mutualistic plant-animal relationships may affect
the survival and continuing evolution of either partner.
Two night-blooming columnar cacti (P. pringlei and Stenocereus thurberi) in Sonora, Mexico, may be falling below
their reproductive potential possibly due to the scarcity of
Leptonycteris bats (Fleming et al. 1996). The population
sizes of three columnar cacti (Cereus repandus, Pilosocereus
lanuginosus, and Stenocereus griseus) growing on Curaçao,
Netherlands Antilles, are decreasing due to unregulated
land development threatening the survival of two species
of nectar-feeding bats (Leptonycteris curasoae curasoae and
Glossophaga longirostris elongata; Petit and Pors 1996).
Conservation efforts should focus not only on endangered
or threatened cacti but also on those animal species that are
essential for their pollination and seed dispersal.
Biodiversity of Cultivated Cacti
Most of the information available on biodiversity of cultivated cacti comes from allozyme studies of two economically important ornamental cacti: Christmas cactus
(Schlumbergera) and Easter cactus (Hatiora; formerly
Rhipsalidopsis). The levels of genetic diversity in these two
cacti (Table 8.2) are similar to those found in the “average”
domesticated crop.Tabe8l2.neahrere:
Chessa et al. (1997) used allozymes to analyze an Italian
collection of 33 prickly pear (Opuntia ficus-indica) clones.
Seven enzyme systems were examined using cladode tissue
and 10 enzyme systems using pollen. Allozyme polymorphism was detected for 2 enzyme systems with cladodes
and 5 enzyme systems with pollen (29% and 50% polymorphism, respectively). Malate dehydrogenase was the
most effective enzyme for distinguishing clones within the
collection. Uzun (1997) examined allozymes in 15 Turkish
ecotypes and 3 Italian cultivars of prickly pear using 7 enzyme systems. All cultivars displayed the same banding
patterns, suggesting that genetic diversity among O. ficusindica ecotypes and cultivars is low. Neither of these allozyme studies provided genetic interpretations of isozyme
banding patterns for O. ficus-indica, which is required for
calculating genetic diversity statistics.
Genetic diversity in cultivated cacti is limited by the restricted number of progenitors and the loss of genetic variation in cultivation. Most of the domesticated cacti grown
for fruit or ornamental flowers apparently originated from
a relatively narrow germplasm base. In the case of Easter
cactus, over 100 distinct clones have been described (Meier
1995), but all probably are descendants of three plants (two
Hatiora gaertneri and one H. rosea) collected in the field in
the late 19th and early 20th centuries. Loss of genetic variation commonly occurs during crop domestication due to
TA B L E 8 . 2
Genetic diversity for clonal germplasm collections of Christmas cactus (Schlumbergera) and Easter cactus (Hatiora)
Plant
Christmas cactus
Easter cactus
Crops
Number of
clones analyzed
Polymorphic
loci (%)
Mean number of
alleles per locus
Mean
heterozygosity
44
40
—
75
54
49
2.17
1.85
2.15
0.24
0.21
0.19
Calculations are based on 12 loci for Christmas cactus and 13 loci for Easter cactus (O’Leary and Boyle 1999, 2000)
or 18 crop plants (Doebley 1989).
culling undesirable plants, random loss of alleles, and
changes in gene frequencies because of adaptation to the ex
situ environment. O’Leary and Boyle (1999) found that a
collection of 40 Easter cactus clones (which included fieldcollected clones, older cultivars, and modern cultivars) exhibit greater genetic diversity than a subset of 13 modern
cultivars that make up the bulk commercial production.
Isozyme profiles indicate that many of the uncommon alleles found in older Easter cactus cultivars are not present
in modern cultivars. Thus, the reduced level of genetic diversity in modern cultivars results from loss of alleles during breeding and selection.
Other factors tend to preserve the level of genetic diversity in cultivated cacti. Unlike crops like corn or wheat
that have been cultivated for millennia, most cultivated
cacti have been domesticated relatively recently and thus
have not been subject to intensive breeding and selection.
For example, field-collected plants of Schlumbergera truncata and H. gaertneri were not introduced into cultivation
until 1818 and 1882, respectively. Also, most of the economically important cacti are propagated asexually (except
for breeding new cultivars), which tends to conserve more
of the initial genetic diversity compared to sexual propagation. Moreover, interspecific hybridization is widely practiced with ornamental cacti and tends to increase the level
of genetic diversity, as for Easter cactus. Most cultivated
clones of Easter cactus are complex interspecific hybrids of
H. gaertneri and H. rosea ( = H. × graeseri [Werderm.]
Barthlott). Although flower color is quite limited in two
progenitor species (H. gaertneri has scarlet-red flowers
whereas H. rosea has rose-pink flowers), the flower color
range in H. × graeseri includes shades of red, lavender, purple, and pink to salmon and golden-orange (Meier 1995).
Although overcollection is a significant problem that
threatens the survival of some wild cacti, the opposite
problem occurs for many cultivated cacti, i.e., low genetic diversity due to insufficient collection from wild sources.
Barthlott et al. (2000) reported that all 58 of the species in
tribe Rhipsalideae are in cultivation, but eight of these
species (Lepisium micranthum, L. miyagawae, Rhipsalis
ewaldiana, R. hoelleri, R. juengeri, R. pentaptera, Schlumbergera kautskyi, and S. orssichiana) are likely to be represented by a single clone. Rhipsalis pentaptera, for example,
is cultivated in more than 100 botanic gardens (Barthlott
et al. 2000), but there is no genetic diversity among cultivated specimens because they are all derived from vegetative propagation of a single plant. The geophyte Echinopsis
chamaecereus (formerly Chamaecereus silvestrii; Fig. 8.1)
represents another case of restricted biodiversity in cultivated cacti. It is one of the most popular ornamental cacti
and is grown for its unique finger-like shoots and bright
scarlet flowers. However, all of the E. chamaecereus plants
in cultivation apparently originated from one self-incompatible clone (R. Kiesling, personal communication). Low
genetic diversity in cultivated cacti restricts the development of new cultivars by conventional plant breeding
techniques.Fgiure8n1.eahrere:
The importance of conserving cultivars of economically useful cacti is often overlooked but needs to be addressed (Given 1994). Cultivars often contain unique assemblages of genes that may be useful for future breeding
efforts. Plant breeders rely primarily on cultivars as the genetic resource for developing new varieties (Frankel et al.
1995). Aside from their economic value, cactus cultivars can
be useful for both applied and basic research because they
often can be cultivated more readily than wild taxa.
Conservation of Cacti
Human activities are having a heavy impact on populations of cacti, whether that impact is the destruction of
natural habitats or overcollecting by hobbyists and commercial dealers. Careless collectors have left piles of dying
cacti in the field (Fig. 8.2), and mining activities, road construction, and farming have destroyed prime habitat for
Biodiversity and Conservation
129
Figure 8.1. Echinopsis chamaecereus (formerly Chamaecereus silvestrii), commonly known as peanut cactus, is a popular ornamental species. The
plants in cultivation apparently originated from vegetative propagation of a single, self-incompatible clone.
rare cacti. Indeed, cactus conservation faces a serious crisis. Unfortunately, there are few easy answers as to how
cacti can be better protected because part of the problem
rests with human nature.Fgiure82.neahrere:
Most people agree that conservation is a good thing.
Unfortunately, many view conservation strictly in the abstract, not in the biological sense, where it properly lies.
Also, there are disagreements among biologists themselves
as to the best ways to conserve wildlife. Both plants and animals are part of the ecosystem; all have a function,
though we may not easily recognize it. One can speak of
the esthetic aspects of wildlife, or of the potential and actual contributions that plants and animals make to human
existence, whether for food, fiber, beauty, medicine, or a
myriad other things. Thus, most accept the fact that plants
and animals should be protected, but some limit that protection to “whenever possible and appropriate.” There are
continuing struggles between conservationists and developers, between those that want to protect wildlife and
those that wish to exploit it. Usually there are not easy answers or simple compromises between these differing par-
130
Boyle and Anderson
ties. Hence, conservation efforts are frequently frustrated
by well-intentioned groups that simply have different
agendas. Education and communication are two critical
tools that must be used if conservation efforts are to have
a chance of success.
Some wish to lock up vast areas of land, restricting access to everyone so that natural populations of plants and
animals will be permanently preserved. Others feel that living organisms should be an available resource for humans,
and that natural events, such as extinction, should be allowed to proceed, especially when coming in conflict with
human activities. Some believe that a few protected areas,
botanic gardens, and zoos will be adequate for preserving
at lease some of the natural diversity. Unfortunately, whatever conservation approach is used, extinction is irreversible, and the lost organism cannot be brought back
again. Interestingly, few would disagree that one of the
main functions of conservation is to perpetuate plants and
animals so that future generations may benefit from them.
The problem is how to best attain that goal. Conservationists often speak of the importance of plants as pos-
Figure 8.2. Pile of dead and dying cacti in the Big Bend Region of
west Texas in 1982. Pictured is Duc Anderson, an assistant on a field
study of rare cacti.
sible sources of drugs or other products of value to humans. Clearly, a strong case can be made for the conservation of plants, including cacti.
Why Cacti Are Threatened
Cacti, like many other plants, are seriously threatened by
habitat destruction, whether for the development of new
farmland, for expanding urban areas, or for other human
activities, such as road building and mining. Many cacti
are also threatened by collectors, who wish to either sell
them in the trade or simply have them in personal collections. Cacti are remarkable organisms, and many people
throughout the world enjoy collecting and propagating
them. Unfortunately, a source of plants for trading or selling is needed, and this source often comes from wild populations. Millions of cacti are artificially propagated annually, thus satisfying many hobbyists (Fig. 8.3). However,
some collectors are like “stamp collectors” in that they want
as many “originals” as possible, meaning that the plants
must come directly from the wild. Thus, uncommon and
unusual cacti are frequently the victims of these unscrupulous collectors, who flout local and national laws to
satisfy their personal needs. Conservationists agree that
habitat destruction is one of the main factors that lead to
extinction, but habitat destruction is only one of the causes of the disappearance of cacti, with illegal collecting
being a significant cause.Fgiure83n.eahrere:
These illegal activities have been frustrating to scientists. A goal of scientific research is to provide an accurate
description of material being studied. However, scientists
have quickly learned that unscrupulous collectors immediately visit sites, the descriptions of which have been published or recorded on herbarium sheets. In several instances
most or all the plants have been removed, e.g. for Aztekium
ritteri. A new locality for this species has been obtained so
that it can be studied (Anderson and Skillman 1984); however, habitat information is presented only in broad generalities because of the threat of collectors, a dilemma faced
by many researchers. In 1996 George Hinton described a
new Mammillaria species, M. luethii. The few plants that
have been seen and the photographs of this unusual cactus
have caused a sensation among collectors, who wish to
learn where it grows. Hinton and Jonas Lüthy, the discoverers of the plant, have not told anyone of its location because of its rarity and the near certainty that unscrupulous
collectors would immediately devastate the wild population if the location were divulged. Rather, the two are arranging for the artificial propagation of the species to make
it available to collectors. Again, however, proper science is
thwarted by the need to protect the wild population.
Cactus researchers have long realized the importance of
cactus conservation. In 1987 the Cactus and Succulent
Specialist Group of the Species Survival Commission
(SSC), an arm of the International Union for Conservation
of Nature and Natural Resources (IUCN), was created.
Scientists from many regions of the world collaborated in
writing Cactus and Succulent Plants: Status Survey and
Conservation Action Plan (Oldfield 1997). This document
is the most comprehensive publication dealing with the
conservation status of each succulent plant group, including cacti, as well as conservation measures and action
proposals.
Determination of Threatened Cacti
The United States Endangered Species Act was passed in
1973 and the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) came
into existence in 1976. There was necessarily a rush to place
plants that people thought were threatened or endangered
on the lists. The first listing of cacti for CITES was creat-
Biodiversity and Conservation
131
Figure 8.3. Commercially grown cacti in California.
ed on 1 July 1975, and in several cases there were few data
to support the decisions that were made. Indeed, the determination of what organism is threatened or endangered
is difficult at best, but a strong effort has been made to create a useful system by which plants and animals are placed
in various categories of threat. Nearly sixty species of cacti
are currently listed in Appendix I of CITES (Table 8.3).
Thirty species are listed as threatened or endangered in the
United States (Table 8.4). This may seem like a small number when considering that there are about 1,700 species of
cacti.Tabe8l3n.eahrereT:abe8l4.neahrere:
Studies of natural cactus populations since 1960 indicate two things: (1) not all seriously threatened cacti are on
either of the lists; and (2) not all the plants on the lists may,
in fact, be seriously threatened with extinction. The United
States government asked scientists at the Desert Botanical
Garden in Phoenix, Arizona, to monitor two species of
cacti in the Big Bend region of Texas that were listed in the
U.S. Endangered Species Act as Threatened: Echinomastus
( = Neolloydia or Sclerocactus) mariposensis and Coryphantha
ramillosa (Anderson and Schmalzel 1997; Schmalzel and
132
Boyle and Anderson
Anderson 1999). Both species were found to be widespread
and not seriously threatened with extinction. Scientists at
the Desert Botanical Garden have also been involved in
evaluating the conservation status of suspected rare cacti in
Mexico, an effort sponsored by the World Wildlife Fund
(Anderson et al. 1994). A joint team of Americans and
Mexicans has been involved in a 3-year, CITES-sponsored
cactus-monitoring project, also in Mexico (Anderson
1997). The two monitoring projects (in Mexico) provided
much information about the conservation status of nearly
50 species of rare cacti.
Two new species of cacti, Aztekium hintonii and Geohintonia mexicana, were described in 1992 just prior to the
beginning of the CITES-sponsored study, so they were initially included in the project because of the excitement
produced among the cactus hobbyists and dealers and uncertainty about their conservation status. The discoverer of
the plants, George Hinton, provided detailed information
about the size and density of the populations of the two
species, which grow sympatrically on gypsum rocks in
Nuevo León. Both species are relatively widespread and
TA B L E 8 . 3
Cacti listed in Appendix I of CITES (as of 1996), including endangered species for which international trade is banned
Ariocarpus (all 6 species)
Astrophytum asterias
Aztekium ritteri
Coryphantha werdermannii
Discocactus (all 5–6 species)
Echinocereus ferreirianus subsp. lindsayi
E. schmollii
Escobaria leei
E. minima
E. sneedii
Mammillaria pectinifera
M. solisioides
Melocactus conoideus
M. deinacanthus
M. glaucescens
M. paucispinus
Obregonia denegrii
Pachycereus militaris
Pediocactus bradyi
P. despainii
P. knowltonii
P. paradinei
P. peeblesianus
P. sileri (Sclerocactus sileri)
P. winkleri
Pelecyphora (both species)
Sclerocactus brevihamatus subsp. tobuschii
S. erectocentrus (Echinomastus erectocentrus)
S. glaucus
S. mariposensis (Echinomastus mariposensis)
S. mesa-verdae
S. papyracanthus
S. pubispinus
S. wrightiae
Strombocactus disciformis
Turbinicarpus (all 6 species)
Uebelmannia (all 2–4 species)
Updated taxonomy appears in parentheses.
numerous. However, extensive illegal collecting of plants
occurred when a few individuals from eastern Europe
learned where they grew, and some local villagers discovered that the plants could be sold and charged visitors for
admission to the sites. Although the local villagers naturally
resented efforts by conservationists to protect the populations and some illegal collectors were apprehended by
Mexican authorities, the devastation of some sites continued. Artificially propagated material of both species is now
available in the market, which has led to a reduction in the
pressure on the wild populations. Ironically, the seeds and
plants used as the source of this artificially propagated material were illegally removed from Mexico. This experience
with A. hintonii and G. mexicana has created an interesting dilemma among hobbyists who have ethical concerns
about the conservation of cacti. Artificially propagated
plants may be purchased, but they are derived from illegally collected stock. However, these plants are also reducing the pressure to collect material from wild populations.
In 1980 the IUCN—The World Conservation Union
(a nongovernmental organization created in Switzerland in
1948) established criteria and categories of threat for assessing extinction risks to species. An improved set of criteria and categories of threat was adopted in 1994, which
were later used to assess some of the species that were listed in the 1996 IUCN Red List of Threatened Animals and
the 1997 IUCN Red List of Threatened Plants. The latter
publication lists 33,798 species as threatened, approximately one-eighth of the world’s total vascular plants
(Walter and Gillett 1998). The Red List has 581 cactus
species, approximately 35% of the total number of species
in the family. Probably this is a fairly accurate estimate of
the conservation threat to the family.
Both category definitions and the criteria by which they
are determined have been controversial, so a further revision
of the categories is currently under way (IUCN/ SSC
Criteria Review Working Group 1999). A system that can
be readily used on plant populations is needed, as the present IUCN categories of threat are difficult to apply unless
extensive fieldwork is undertaken. The following are the
proposed new categories of threat: (1) Extinct—no organisms exist; (2) Extinct in the Wild—organisms still exist but
no longer in the wild; (3) Critically Endangered—facing an
extremely high risk of extinction in the near future; (4)
Endangered — not critically endangered but facing a very
high risk of extinction in the wild in the near future; (5)
Vulnerable—facing a high risk of extinction in the wild; (6)
Near Threatened—do not qualify as threatened but may become so in the near future; (7) Least Concern—do not qualify for any of the above categories. The criteria are based on
the evaluation of populations of organisms and a projection
of their probable future, but data are often simply
insufficient to classify the organism into one group or another. This is particularly true of the cacti, for few detailed,
long-term studies have been carried out, underscoring one
of the highest priorities for cactus conservation.
Conservation of Specific Cacti
Cacti have long been — and continue to be — used by
Native Americans in both North and South America.
Though their use as food sources has decreased with the
improvement of transportation and Native Americans’ par-
Biodiversity and Conservation
133
TA B L E 8 . 4
Cacti listed as Endangered (E) or Threatened (T) in the U.S.
Endangered Species Act (as of 1998), with updated taxonomy
in parentheses
Taxon
Ancistrocactus tobuschii (Sclerocactus brevihamatus)
Astrophytum asterias
Cereus eriophorus var. fragrans (Harrisia fragrans)
Coryphantha minima (Escobaria minima)
C. ramillosa
C. robbinsorum (Escobaria robbinsorum)
C. scheeri var. robustispina (C. robustispina)
C. sneedii var. leei (Escobaria sneedii subsp. leei)
C. sneedii var. sneedii (Escobaria sneedii subsp. sneedii)
Echinocactus horizonthalonius var. nicholii
Echinocereus chisoensis var. chisoensis
E. fendleri var. kuenzleri
E. lloydii (E. × roetteri)
E. reichenbachii var. albertii (E. reichenbachii subsp. fitchii)
E. triglochidiatus var. arizonicus (E. coccineus)
E. viridiflorus subsp. davisii
Echinomastus mariposensis
Harrisia portoricensis
Leptocereus grantianus
Opuntia treleasei (O. basilaris var. treleasei)
Pediocactus bradyi
P. despainii
P. knowltonii
P. peeblesianus var. peeblesianus
P. sileri (Sclerocactus sileri)
P. winkleri
Pilosocereus robinii (P. polygonus)
Sclerocactus glaucus
S. mesa-verdae
S. wrightiae
Status
E
E
E
E
T
T
E
E
E
E
T
E
E
E
E
E
T
T
E
E
E
E
E
E
E
T
E
T
T
E
tial acculturation into Anglo and other societies, cacti still
have importance for ceremonial and medicinal purposes.
Central Mexico provides an example of local use of plants
having a negative impact on wild populations. Two species
of Mammillaria, M. bocasana and M. plumosa, are used locally for Christmas decorations, which has led to the complete destruction of some populations of these cacti near
villages (W. A. Fitz Maurice, personal communication).
The peyote cactus (Lophophora williamsii) is a more serious example of the impending loss of a cactus in a specific
region that is important in Native American culture. This
plant is believed by many Native Americans to be a gift
from God and a sacrament, and the tops of the plant are
134
Boyle and Anderson
consumed as part of the religious ceremony of the Native
American Church (Anderson 1995, 1996). The stems of this
cactus contain the alkaloid mescaline, which affects the
senses, often leading to enhanced visual experiences.
Thousands of fresh or dried “buttons” (Fig. 8.4) are consumed annually in these all-night ceremonies involving
singing, praying, and quiet contemplation. Hispanic “peyoteros,” who are licensed by the state of Texas, harvest large
quantities of the tops of the plants and sell them legally to
Native Americans throughout the United States and
Canada. Their harvesting technique is ecologically sound in
that only the upper part of the stem is removed, thus allowing the remaining portion of the plant to develop new
stems or “heads.” Historically, the peyoteros have visited the
populations only rarely, allowing the plants to regenerate,
but this is not the case today. Although peyote is widespread
throughout northern Mexico and parts of Texas, its distribution in the United States is limited mostly to privately
owned fenced areas along the Rio Grande.Fgiure84.neahrer:
As many as a million tops of the plants are harvested
for use in the peyote ceremony each year, thus creating a
severe demand on the populations of peyote in southern
Texas. The supply of the cactus in the United States is becoming scarce because not all populations are available for
periodic harvest. Some landowners have erected high
fences and introduced exotic game animals, creating game
ranches. Anyone, often including peyoteros, is strictly prohibited from coming onto their property unless they have
paid a fee to hunt the game animals. This prohibition also
includes Native Americans, who wish to go to the “peyote
gardens” to collect plants for their personal use (Anderson
1995). An additional problem is that other ranchers, wishing to improve grazing for their cattle, destroy the native
plants, including peyote, with giant root plows in order to
plant exotic grasses. Thus, more and more people want to
collect tops from the small, available populations on
ranches where entry is permitted and the harvesting of
peyote buttons is allowed, thus creating a serious conservation crisis. A possible solution is to allow the collection
of peyote in neighboring Mexico, but both Mexican and
U.S. regulations prohibit this. The only other alternative is
the commercial propagation of peyote, but this has not yet
been tried extensively and there is some question about its
legality. Unfortunately, some Native Americans believe that
cultivated peyote plants are not as “good” as those from the
wild.
Importance of Artificial Propagation
CITES specifically defines artificially propagated plants as
those that are grown from seeds, cuttings, or propagules
Figure 8.4. Drying tops of the peyote cactus (Lophophora williamsii).
under “controlled conditions” (Oldfield 1997). The stock
or source of these artificially propagated plants has been established and is maintained in such a way that the survival
of the species in the wild is not adversely affected. Such
propagation programs should operate so that the stock organisms can be maintained indefinitely. The Convention
of CITES specifically states that artificially propagated
Appendix I plants (Table 8.3) may be treated in exactly the
same way as Appendix II plants, which in many countries
means that they are treated as non-CITES plants.
However, most countries require some documentation on
the origin of the plants. Unfortunately, in many cases this
documentation is both expensive and extensive, which discourages many nursery operators from propagating
Appendix I species.
Clearly, the impact of illegal collecting on wild populations of cacti could be greatly reduced if large numbers
of quality plants were made available in the trade at a reasonable cost. Most hobbyists want to have specimens of a
certain cactus, and they are willing to purchase well-grown
artificially propagated plants because they know that they
are both protecting the wild populations and are getting
plants that have a better chance of survival in cultivation.
However, there will always be a few individuals who feel
they must have plants from the field. One of the most important conservation actions that can be taken is for
CITES and other government agencies to encourage and
facilitate the artificial propagation of Appendix I cacti.
CITES
CITES is the primary international legislation that deals
with the conservation of plants and animals; it now has
over 130 member countries. CITES regulates trade in
threatened wild species, placing them in one of three appendices. Those species listed in Appendix I (Table 8.3) are
considered to be at serious risk due to international trade;
thus, trade in these organisms between signatory countries
is banned. Appendix II lists those species that may be
threatened by excessive amounts of trade, but for which
trade is permitted with appropriate licenses, monitoring,
and certain controls; all cacti not in Appendix I are listed
in Appendix II. Appendix III is used by countries that
Biodiversity and Conservation
135
want to control trade in certain plant or animal species that
are not currently in either of the other appendices. CITES
appendices are amended by agreement of member countries at the biennial Conference of the Parties, which usually follows the consideration of detailed proposals that
have been submitted by one or more members. Thus,
changes to the listings in the appendices do occur, though
the process is cumbersome. For example, Leuchtenbergia
principis was originally listed in Appendix I, but because research showed that clearly it was not threatened with immediate extinction, it was transferred to Appendix II.
Some cacti presently listed in Appendix I may not be as severely threatened as some that are not on the list, but only
detailed research and extensive paperwork can make these
changes.
CITES is implemented through laws and administrative procedures of member countries, as the actual
Convention provides only the framework for implementation. International cooperation between member countries is essential. Each country has Scientific and Management Authorities that are responsible for implementing the
Convention. In the United States, for example, these authorities are within the U.S. Fish and Wildlife Service.
Standardized implementation of CITES and national regulations, especially among plants, is spotty at best. Bureaucratic red tape and regulations can inhibit the effective implementation of the CITES regulations. For instance,
artificially propagated CITES plants are regulated by massive amounts of regulation and heavy financial burdens on
the growers. Also, CITES was established to regulate the
trade of threatened plants and animals, but legislative restrictions have created bureaucracies for legitimate scientific research. Researchers at the Desert Botanical Garden
received the appropriate collecting and export permits to
do field work in Peru and to collect a specific number of
research specimens, but someone mistakenly reduced the
number of plants that could be exported. Upon the arrival
of the shipment into the United States, the inspector
confiscated the “extra” plants. Although the export permit
was eventually corrected by Peruvian authorities, U.S. authorities would not accept the corrected forms because
they were on the wrong letterhead. The collection data
were not allowed to accompany the confiscated plants, so
their scientific value was lost.
Distinctions should be made between scientists doing
research on cacti and those trading in these plants. Of
course, scientists, like everyone else, are subject to the laws
of whatever country in which they are working. Indeed,
CITES has made some provision for facilitating research,
as institutions may apply for permits and register with the
136
Boyle and Anderson
CITES Secretariat as Institutions for Scientific Exchange.
These permits authorize the non-commercial shipment of
legally acquired specimens between registered institutions.
The system can work well, but unfortunately some countries have few, if any, registered scientific institutions.
Research is a critically important part of plant conservation, for rare plants must be understood so they may be appropriately protected, and cumbersome regulations and
permit procedures can frustrate this endeavor.
The Convention on Biological Diversity
In 1992, 153 countries signed the Convention on Biological
Diversity, with the objectives being “the conservation of biological diversity, the sustainable use of its components,
and the fair and equitable sharing of the benefits arising
out of the utilization of genetic resources” (Oldfield 1997).
One of the most important aspects of this Convention is
that member countries are required to identify those components of biological diversity important for both conservation and sustainable use. The Convention also requires
the establishment of protected areas and the passing of appropriate legislation to protect those organisms found to be
threatened. This Convention certainly contains appropriate elements for assisting conservation groups and agencies
in implementing programs to protect threatened organisms, including cacti, and by helping them identify threatened species and developing measures to protect them.
In situ and ex situ Conservation
Even with agreement that threatened organisms need to be
conserved and programs need to be developed to ensure
their perpetuation, the ideal solutions may not be attainable. Two different approaches to the conservation of organisms are in situ and ex situ conservation. In situ conservation is simply protecting wild plants and animals in their
natural habitats. Clearly, this is the best insurance that rare
and threatened species will have a long-term chance of survival. Usually in situ conservation involves setting aside
areas and establishing legal protections, such as those
found in nature preserves, national parks, and protected
private lands. It may also include habitat restoration and
even the reintroduction of organisms. The U.S. Endangered Species Act provides protection for certain “critical
habitats” defined as being essential for the long-term conservation of the threatened or rare species, including cacti.
Ex situ conservation is a second level of protecting rare and
endangered plants away from their natural habitats.
State and national parks, preserves, and monuments are
significant regions in which threatened cacti can be protected. Three national parks or preserves in the western
Figure 8.5. Mojave Natural Preserve Desert National Park in California.
United States have significant numbers of cacti: Joshua Tree
National Park in California; Mojave Natural Preserve
Desert, also in California (Fig. 8.5); and Big Bend National
Park in Texas. Arizona has two national monuments specifically set aside to protect cacti: Saguaro National Monument
and Organ Pipe Cactus National Monument. A significant
conservation effort in South America is Pan de Azúcar
National Park in Chile, in which important cacti of the
Atacama Desert are protected. Although the Galápagos
Islands of Ecuador are commonly thought of as having unusual animals, the Archipelago also has many remarkable
species of plants, including cacti, that are protected through
its status as a national park (Fig. 8.6). Mexico has established several preserves for the protection of cacti: Pinacate
Reserve in Sonora, Mapimí Nature Reserve in Chihuahua,
Cañon de Huasteco in Nuevo León, Parque Internacional
del Río Bravo also in Nuevo León, and Isla Cedros Sanctuary in Baja California.Fgiure85n.eahrereF:giure86.neahrere:
Private owners of land containing threatened cacti may
also knowingly or unknowingly protect the native plants
by prohibiting access. The Trans Pecos Heritage Associ-
ation in west Texas has no conservation agenda in prohibiting access to its vast ranches; it simply does not want
trespassers of any kind on its properties. Some of these
ranches have populations of rare cacti, such as Echinocereus
viridiflorus subsp. davisii, which are protected from collectors by the trespassing regulations. On the other hand,
researchers are often prohibited from studying the plants
as well. The Nature Conservancy consciously purchases
tracts of land for the protection of wild species, and often
this land remains in private ownership.
Despite national and local efforts to set aside critical
habitats containing rare or threatened species, some people will still trespass and illegally remove plants, such as
cacti, and thus adversely affect the long-term future of the
populations. Both Saguaro National Monument and Big
Bend National Park have suffered losses in their cacti populations from illegal activities.
Ex situ conservation is the cultivating of rare and
threatened plants “away from danger” in botanic gardens
and other places that are not the natural habitats of the
plants involved. This type of conservation is clearly not as
Biodiversity and Conservation
137
Barcelona, Spain, which was founded by Fernando Riviere
de Caralt and is now operated by his family. Steven
Brack’s Mesa Garden in Belen, New Mexico, is an excellent
example of a commercial business that propagates and sells
documented materials obtained from legally collected
stock plants. These plants provide additional specimens for
both hobbyists and botanic gardens. Seed banks are also
important in ex situ plant conservation, but few contain
more than just a small fraction of cactus species. The exception is the seed collection of the Desert Botanical
Garden, which has seeds of several hundred cactus species.
Conclusions and Future Prospects
Figure 8.6. Forest of Opuntia echios var. barringtonensis in the
Galápagos Islands, Ecuador.
effective as the in situ conservation of wild populations, but
the latter may be impossible because of habitat fragmentation and destruction, political pressures against conservation, legal prohibitions, bureaucratic obstacles, overcollection, and inaccessibility because of private ownership.
Thus, ex situ conservation may be the only viable alternative in protecting and perpetuating a threatened plant.
Effective ex situ conservation requires careful documentation, the propagation of sufficient numbers of individuals
to ensure some degree of genetic diversity, and the prevention of diseases. Botanic gardens, such as the Desert
Botanical Garden (Phoenix, Arizona), the Huntington
Botanical Garden (San Marino, California), the Jardín
Botanico del Instituto de Biologia de Universidad
Nacional Autónoma de México (UNAM) in Mexico City,
the Exotic Garden of Monaco, and the Zürich Succulent
Collection in Switzerland are examples of gardens emphasizing the collection and growing of cacti. Other examples
of ex situ conservation of cacti are private collections and
some commercial nurseries. An example of the former is
the Jardín Botanico Tropical “Pinya de Rosa” near
138
Boyle and Anderson
Although cacti inhabit a wide diversity of climatic regions
and ecosystems throughout the New World, approximately half of all known species of cacti occur in four geographical regions, or centers of diversity. Among the four
centers of diversity, the geographical region comprising
Mexico and the southwestern United States contains the
greatest concentration of species and the most endemic
genera. In this regard, cacti have been the subjects of few
allozyme surveys, and therefore relatively little information
is available on the extent and distribution of biodiversity
within individual species. Strategies for the conservation of
rare or threatened cacti would be aided by more information on the extent of genetic variation within their populations. Kay and John (1996) have recommended studies of
each rare or threatened species to discern the extent and
distribution of genetic diversity. Their rationale for the recommendation was that inferences from presumably similar ecological situations appear to be inapplicable. This
would be impractical for all rare or threatened cacti because of time and cost constraints. Gray (1996) reviewed
the literature on genetic diversity in natural plant populations and concluded that genetic diversity in most plant
species extends along the axis of habitat variability. Based
on this finding, Gray (1996) proposed that, in the absence
any genetic diversity data, as many populations as feasible
across the species’ entire geographic range should be conserved, protected in situ, or sampled for ex situ collections.
The amount of genetic diversity within a plant species
is ultimately limited by the effective size of the populations. Wild species lose genetic diversity due to reductions
in effective population size or population fragmentation.
How reduced genetic diversity actually affects populations
of wild cacti is not known and requires study. With regard
to cultivated cacti, information on the genetic diversity of
Hatiora and Schlumbergera is available but less is known
about biodiversity of other economically important cacti.
Efforts should therefore be made to estimate the genetic
variation present in germplasm collections of other cultivated cacti. The economically important cacti and their
wild relatives should receive a high priority for conservation efforts. Germplasm of cultivated cacti and their near
relatives should be collected and preserved ex situ. Seeds
should be collected from several populations throughout
the species’ natural ranges. Further work may be needed to
devise protocols for the long-term storage of seeds, pollen,
and vegetative material.
The present is a critical period with regard to the future
of cactus conservation, and the outlook is grim. It will be
impossible to halt—or even slow down—habitat destruction as long as the human population grows at its current
rate, especially in Latin America. Urban areas will expand
and marginal land will be converted to farmland. Illegal
collecting will continue. Hopefully, the efforts of national
societies and the International Organization for Succulent
Plant Study (IOS) to educate its members—and the public as a whole — may gradually produce a better understanding of the need for cactus conservation. Some national cactus and succulent organizations have already
done a great service to conservation in prohibiting the entering of field-collected plants in sanctioned shows and
competitions. Several commercial nurseries are also providing excellent plants for hobbyists.
CITES and the implementation of its regulations has
been and will continue to be effective in controlling trade
and its impact on wild populations, but excessive bureaucracy will frustrate and discourage many people from
cooperating fully. Hopefully, CITES and the many governmental agencies concerned with conservation will
streamline their permitting procedures. A number of projects were proposed in the Cactus and Succulent Action
Plan (Oldfield 1997). Funding is necessary for extensive
field work and monitoring so that the rare and threatened
cacti may be better understood. More knowledge will enable researchers and others to manage and protect wild
populations for the foreseeable future. Cacti are amazing
plants and extensive conservation efforts are certainly
justified. Cooperative effort among researchers, conservationists, and hobbyists is required to ensure that wild populations of cacti will continue to exist.
Literature Cited
Anderson, E. F. 1995. The “peyote gardens” of south Texas:
A conservation crisis. Cactus and Succulent Journal
(U.S.) 67: 67–73.
Anderson, E. F. 1996. Peyote: The Divine Cactus, 2nd ed.
University of Arizona Press, Tucson.
Anderson, E. F. (compiler). 1997. CITES Project S-53:
Population Study on Rare Mexican Cacti. A report to
CITES on a three-year monitoring and study project.
Desert Botanical Garden, Phoenix, Arizona.
Anderson, E. F., and R. Schmalzel. 1997. Joint Task Force
Six Lloyd’s Mariposa Cactus Survey (Sclerocactus
[Neolloydia] mariposensis). A report to JTF-6 on a
three-year study of this rare cactus of Texas and
Mexico. Desert Botanical Garden, Phoenix, Arizona.
Anderson, E. F., and S. M. Skillman. 1984. A comparison
of Aztekium and Strombocactus (Cactaceae). Systematic
Botany 9: 42–49.
Anderson, E. F., N. P. Taylor, and S. Arias Montes. 1994.
Threatened Cacti of Mexico. Royal Botanic Gardens,
Kew, United Kingdom.
Barbier, E. B. 1997. Ecological economics, uncertainty and
implications for policy setting priorities for biodiversity conservation. In Investing in Biological Diversity: The
Cairns Conference (OECD Proceedings). Organization
for Economic Co-operation and Development, Paris.
Pp. 115–140.
Barthlott, W. 1983. Biogeography and evolution in neo- and
paleotropical Rhipsalinae (Cactaceae). In Dispersal
and Distribution (K. Kubitzki, ed.). Sonderbände des
naturwissenschaftlichen Vereins in Hamburg 7. Verlag
Paul Parey, Hamburg. Pp. 241–248.
Barthlott, W., and D. R. Hunt. 1993. Cactaceae. In The
Families and Genera of Vascular Plants. Vol. II: Flowering Plants (K. Kubitzki, ed.). Springer-Verlag, Berlin.
Pp. 161–197.
Barthlott, W., G. Rauer, P. L. Ibisch, M. von den Driesch,
and W. Lobin. 2000. Biodiversität und botanische
Gärten. In Botanische Gärten und Biodiversität
(Bundesamt für Naturschutz, Bonn, ed.). Landwirtschaftsverlag, Munich. Pp. 1–24, 69–70.
Boyle, T. H. 1997. The genetics of self-incompatibility in
the genus Schlumbergera (Cactaceae). Journal of
Heredity 88: 209–214.
Brown, A. H. D., and J. D. Briggs. 1991. Sampling strategies for genetic variation in ex-situ collections of endangered plant species. In Genetics and Conservation of
Rare Plants (D. A. Falk and K. E. Holsinger, eds.).
Oxford University Press, New York. Pp. 99–122.
Chessa, I., G. Nieddu, P. Serra, P. Inglese, and T. La
Mantia. 1997. Isozyme characterization of Opuntia
species and varieties from Italian germplasm. Acta
Horticulturae 438: 45–52.
Biodiversity and Conservation
139
del Castillo, R. F. 1986. La selección natural de los sistemas
de cruzamiento en Opuntia robusta. Master’s Thesis,
Colegio de Postgraduados, Chapingo, Mexico.
M. T. Clegg, A. L. Kahler, and B. S. Weir, eds.).
Sinauer Associates, Sunderland, Massachusetts. Pp.
43–63.
de Nettancourt, D. 1977. Incompatibility in Angiosperms.
Monographs on Theoretical and Applied Genetics 3.
Springer-Verlag, Berlin.
Hamrick, J. L., and M. J. W. Godt. 1996. Effects of life history traits on genetic diversity in plant species.
Philosophical Transactions of the Royal Society of London,
Series B, Biological Sciences 351: 1291–1298.
Doebley, J. 1989. Isozymic evidence and the evolution of
crop plants. In Isozymes in Plant Biology (D. E. Soltis
and P. S. Soltis, eds.). Dioscorides Press, Portland,
Oregon. Pp. 165–191.
Ellstrand, N. C., and M. L. Roose. 1987. Patterns of genotypic diversity in clonal plant species. American Journal
of Botany 74: 123–131.
Fleming, T. H., S. Maurice, S. L. Buchmann, and M. D.
Tuttle. 1994. Reproductive biology and relative male
and female fitness in a trioecious cactus, Pachycereus
pringlei (Cactaceae). American Journal of Botany 81:
858–867.
Fleming, T. H., M. D. Tuttle, and M. A. Horner. 1996.
Pollination biology and the relative importance of nocturnal and diurnal pollinators in three species of
Sonoran Desert columnar cacti. The Southwestern
Naturalist 41: 257–269.
Hunt, D. R. 1992. CITES Cactaceae Checklist. Royal
Botanic Gardens and International Organization for
Succulent Study (IOS), Kew, United Kingdom.
Innes, C., and C. Glass. 1991. Cacti. Portland House, New
York.
IUCN/SSC Criteria Review Working Group. 1999. IUCN
red list criteria review provisional report: Draft of the
proposed changes and recommendations. Species 31/32:
43–57.
Frankel, O., A. H. D. Brown, and J. J. Burdon. 1995. The
Conservation of Plant Biodiversity. Cambridge University Press, Cambridge.
Kay, Q., and R. John. 1996. Patterns of variation in relation
to the conservation of rare and declining plant species.
In The Role of Genetics in Conserving Small Populations
(T. J. Crawford, T. Spencer, D. Stevens, T. E. Tew,
M. B. Usher, and J. Warren, eds.). Joint Nature Conservation Committee, Peterborough, United Kingdom.
Ganders, F. R., and H. Kennedy. 1978. Gynodioecy in
Mammillaria dioica (Cactaceae). Madroño 25: 234.
Meier, E. 1995. Easter cacti (Rhipsalidopsis; Cactaceae).
Haseltonia 3: 10–24.
Gates, H. E. 1932. Lophocereus in lower California. Cactus
and Succulent Journal (U.S.) 3: 136–137.
Murawski, D. A., T. H. Fleming, K. Ritland, and J. L.
Hamrick. 1994. Mating system of Pachycereus pringlei:
An autotetraploid cactus. Heredity 72: 86–94.
Given, D. R. 1994. Principles and Practice of Plant
Conservation. Timber Press, Portland, Oregon.
Grant, V., and K. A. Grant. 1979. The pollination spectrum in the southwestern American cactus flora. Plant
Systematics and Evolution 133: 29–37.
Grant, V., and P. D. Hurd. 1979. Pollination of the southwestern opuntias. Plant Systematics and Evolution 133:
15–28.
Gray, A. 1996. Genetic diversity and its conservation in
natural populations. Biodiversity Letters 3: 71–80.
Hamrick, J. L. 1989. Isozymes and the analysis of genetic
structure in plant populations. In Isozymes in Plant
Biology (D. E. Soltis and P. S. Soltis, eds.). Dioscorides
Press, Portland, Oregon. Pp. 87–105.
Hamrick, J. L., and M. J. W. Godt. 1989. Allozyme diversity in plant species. In Plant Population Genetics,
Breeding, and Genetic Resources (A. H. D. Brown,
140
Hoffmann, M. T. 1992. Functional dioecy in Echinocereus
coccineus (Cactaceae): Breeding system, sex ratios, and
geographic range of floral dimorphism. American
Journal of Botany 79: 1382–1388.
Boyle and Anderson
Nei, M. 1973. Analysis of gene diversity in subdivided populations. Proceedings of the National Academy of Sciences
U.S.A 70: 3321–3323.
Oldfield, S. (compiler). 1997. Cactus and Succulent Plants:
Status Survey and Conservation Action Plan. IUCN/
SSC Cactus and Succulent Specialist Group. International Union for Conservation of Nature and Natural
Resources, Gland, Switzerland, and Cambridge,
United Kingdom.
O’Leary, M. C., and T. H. Boyle. 1999. Cultivar identification and genetic diversity within a Hatiora (Cactaceae) clonal germplasm collection. Journal of the
American Society for Horticultural Science 124: 373–376.
O’Leary, M. C., and T. H. Boyle. 2000. Diversity and distribution of isozymes in a Schlumbergera (Cactaceae)
clonal germplasm collection. Journal of the American
Society for Horticultural Science 125: 81–85.
Olesen, J. M., and S. K. Jain. 1994. Fragmented plant populations and their lost interactions. In Conservation
Genetics (V. Loeschcke, J. Tomiuk, and S.K. Jain, eds.).
Birkhäuser Verlag, Basel. Pp. 417–426.
Olmstead, R. G. 1986. Self-incompatibility in light of population structure and inbreeding. In Biotechnology and
Ecology of Pollen (D. L. Mulcahy, G. B. Mulcahy, and
E. Ottoviano, eds.). Springer-Verlag, New York.
Parfitt, B. D. 1985. Dioecy in North American Cactaceae.
Sida 11: 200–206.
Parker, K. C., and J. L. Hamrick. 1992. Genetic diversity
and clonal structure in a columnar cactus, Lophocereus
schottii. American Journal of Botany 79: 86–96.
Petit, S., and L. Pors. 1996. Survey of columnar cacti and
carrying capacity for nectar-feeding bats on Curaçao.
Conservation Biology 10: 769–775.
Porsch, O. 1938. Das Bestäubungsleben der Kakteenblüte.
Jahrbuch der Deutsche Kakteen-Gesellschichte I: 1–80.
Porsch, O. 1939. Das Bestäubungsleben der Kakteenblüte.
Jahrbuch der Deutsche Kakteen-Gesellschichte II: 81–142.
Redford, K. H., A. Taber, and J. A. Simonetti. 1990. There
is more to biodiversity than tropical rain forests.
Conservation Biology 4: 328–330.
Reid, W. V., J. A. McNeely, D. B. Tunstall, D. A. Bryant,
and M. Winograd. 1993. Biodiversity Indicators for
Policy-Makers. World Resources Institute, Washington,
D.C.
Rowley, G. 1980. Pollination syndromes and cactus taxonomy. Cactus and Succulent Journal (Great Britain) 42:
95–98.
Sahley, C. T. 1996. Bat and hummingbird pollination of an
autotetraploid columnar cactus, Weberbauerocereus we-
berbaueri (Cactaceae). American Journal of Botany 83:
1329–1336.
Schlindwein, C., and D. Wittmann. 1997. Stamen movements in flowers of Opuntia (Cactaceae) favour
oligolectic pollinators. Plant Systematics and Evolution
204: 179–193.
Schmalzel, R., and E. F. Anderson. 1999. Study of the
Bunched Cory Cactus (Coryphantha ramillosa Cutak). A
report to the U.S. Fish and Wildlife Service, Washington, D.C.
Taylor, N. P. 1997. Cactaceae. In Cactus and Succulent
Plants—Status Survey and Conservation Action Plan (S.
Oldfield, compiler). International Union for Conservation of Nature and Natural Resources, Gland, Switzerland, and Cambridge, United Kingdom. Pp. 17–20.
Uzun, I. 1997. Fruit and cladode isozymes in cactus pear.
Acta Horticulturae 438: 45–52.
Wallace, R. S., and D. E. Fairbrothers. 1986. Isoelectrically
focussed seed proteins of populations of Opuntia humifusa (Raf.) Raf. (Cactaceae). Biochemical Systematics
and Ecology 14: 365–369.
Walter, K. S., and H. J. Gillett (eds.). 1998. 1997 IUCN Red
List of Threatened Plants. International Union for
Conservation of Nature and Natural Resources and
The World Conservation Union, Gland, Switzerland.
Wilcox, B. A. 1984. In situ conservation of genetic resources: Determinants of minimum area requirements.
In National Parks, Conservation, and Development: The
Role of Protected Areas in Sustaining Society ( J. A.
MacNeely and K. R. Miller, eds.). Smithsonian Institution, Washington, D.C. Pp. 639–647.
Biodiversity and Conservation
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CHAPTER
›9‹
M E S OA M E R I C A N D O M E S T I C AT I O N
AND DIFFUSION
Alejandro Casas and Giuseppe Barbera
Introduction
Human-Cactus Interactions in Mesoamerica
Archaeological and Historical Evidence
Ethnobotanical Information
Domestication of Cacti
Columnar Cacti
Opuntia Species
Diffusion of Cacti to Other Regions of the World
Colonial Period
Spread of Platyopuntias in Europe
History of Name
Naturalization in Europe
Platyopuntias in Sicily
Expansion to Other Mediterranean Regions
Conclusions and Future Prospects
Literature Cited
Introduction
Mesoamerica, the culturally defined region from central
Mexico to northwestern Costa Rica (Fig. 9.1), is one of the
most important centers of domestication of plants in the
world (Harlan 1975). Archaeological studies suggest that
domestication of plants in this region was initiated approximately 9,600 years ago (Flannery 1986). Dressler
(1953) estimated that about 100 cultivated species (e.g.,
maize, beans, squashes, tomatoes, avocados, and prickly
pears) were domesticated by pre-Columbian cultures of
Mesoamerica. Actually, ethnobotanical studies indicate
that several hundred species were domesticated, some
only in a beginning stage and others to advanced stages,
but many have been poorly studied because they are only
regionally or locally important. Apart from Opuntia spp.
and Hylocereus undatus, cacti are commonly omitted from
checklists of domesticated plants. Nevertheless, archaeologists have revealed that several species of Opuntia as well
as columnar and barrel cacti were among the most important plant resources utilized by humans in prehistoric
Mesoamerica (Callen 1967; Smith 1967, 1986), and ethnobotanists have documented that dozens of species of
cacti are currently utilized by indigenous peoples of this
143
ZACATECAS
NAYARIT
SAN LUIS
POTOSÍ
JALISCO GUANAJUATO
QUERÉTARO
HIDALGO
COLIMA
MICHOACÁN
YUCATÁN
VE
PUEBLA
GUERRERO
AC
CAMPECHE
R
Mexico City
MORELOS
TLAXCALA
MEXICO
RU
Z
QUINTANA
ROO
TABASCO
OAXACA
CHIAPAS
Mesoamerica
Figure 9.1. Mesoamerica and states for the Mexican part of the region (Matos 1994; Vargas 1994).
area, indicating that several species of Opuntia and columnar cacti are in advanced stages of domestication (Colunga
1984; Casas et al. 1997a, 1999a, b).Fgiure9n1.eahrere:
Domestication is an evolutionary process resulting
from the manipulation of living organisms by humans. In
this process, humans select and breed phenotypes with
characteristics they consider advantageous; i.e., individual
plants with better qualities as food, medicine, and other
uses. Other evolutionary forces, such as genetic drift, also
intervene in the selection process and may be significant in
small populations caused by humans due to the isolation
of individual plants or resulting from perturbation and
fragmentation of natural habitats or transplantation of
wild individuals into human habitats. Throughout history, human migrations, displacement of human settlements, commerce, cultural exchange, as well as conquest
and colonization of new areas have been accompanied by
the movement of plant and animal populations (individ-
144
Casas and Barbera
uals and/or their propagules) from one region to another.
In some cases, migration has isolated individuals from their
parental populations, and the influence of artificial selection under different cultural and environmental contexts
can determine particular routes of domestication. In other
cases, migration has reestablished contact between variants
previously separated by natural or human processes, giving
rise to new combinations of genes that are available for
artificial selection. Through domestication, plant populations become morphologically, physiologically, and/or behaviorally divergent from their wild ancestors (Darwin
1868; Harlan 1992). But, as in general evolutionary processes, the inherited, genetically controlled divergence can be
considered a domestication process.
Domestication of plants has generally been associated
with cultivation (Harlan 1992), because artificial selection
more probably occurs under successive generations of harvest and propagation of the desired phenotypes. However,
domestication can also act under different forms of manipulation of wild plant populations in situ, including
species of cacti (Casas et al. 1997b). Indeed, Mesoamerican
indigenous peoples commonly practice a broad spectrum
of interactions with plants (Alcorn 1984; Colunga 1984;
Bye 1993; Casas et al. 1996, 1997a). Casas et al. (1996)
group these forms of plant management into those occurring in situ (in the wild) and those occurring ex situ.
Through interactions in situ, humans may take products
from nature without significant perturbations, but they
may also alter the structure of plant populations by increasing the quantity of target species or particular phenotypes. The main interactions in situ are: (1) gathering,
which is the taking of useful plant products directly from
natural populations; (2) tolerance, including practices directed to maintain, within human-made environments,
useful plants already occurring there; (3) enhancement, directed to increase the population density of useful plant
species, including the sowing of seeds or the intentional
propagation of vegetative structures in places occupied by
wild plant populations; and (4) protection, which includes
conscious activities, such as the elimination of competitors
or predators, fertilization, and pruning to safeguard critical wild plants. Plant management ex situ includes interactions taking place outside natural populations, in habitats created and controlled by humans, including: (1)
transplantation of entire individuals and (2) sowing and
planting of sexual or vegetative propagules (Casas et al.
1996, 1997a,b).
Variation among the species composing a plant community or the individuals forming a population, their
differences in quality as useful resources, and the selective
attitude of humans in taking advantage of some species
and particular individuals and not others are the most important principles in artificial selection of plants. Ethnobotanical studies in Mesoamerica have revealed that this
attitude is common among indigenous peoples and that it
occurs under different interactions between humans and
plants, not only under cultivation. In gathering, people
usually make choices among individual plants based on
their quality as a food, such as flavor, size, color, and presence of toxic substances (Casas et al. 1996, 1997a, 1999b).
This selection may give rise to other types of interaction
involving domestication. When they are found during the
clearing of forest areas, the edible wild plant species and
the preferred variants may be spared, enhanced, and/or
protected in situ, whereas those species and variants whose
edible parts are not preferred by people are eliminated.
Over the long term such selective attitudes may modify
vegetation patches in which the phenotypes desirable to
humans have a better opportunity to be components of
the community, and the selected components may increase
their frequency in populations, another facet of plant
domestication.
This chapter examines cultural and biological aspects
related to the use and management of cacti among peoples
of Mexican Mesoamerica and analyzes how domestication
is occurring in some species. Comparisons of morphology
between wild and manipulated populations of Opuntia
and Stenocereus species are used to illustrate patterns of
artificial selection and evolutionary trends resulting from
domestication under different forms of management. This
information is discussed to determine how domestication
might be occurring in other cacti. The diffusion of cacti,
especially platyopuntias, into other regions of the world is
reviewed to examine trends in domestication of these
species outside of Mesoamerica.
Human-Cactus Interactions in Mesoamerica
Archaeological and Historical Evidence
Mexico is apparently the richest area for cactus species in
the world (Bravo-Hollis 1978). Cacti are among the main
components of the tropical deciduous and thorn-scrub
forests of subhumid tropics as well as arid and semiarid
zones, which cover nearly two-thirds of the country
(Toledo and Ordóñez 1993). Archaeological studies in the
Tehuacán Valley, Puebla (MacNeish 1967), and at Guilá
Naquitz, Oaxaca (Flannery 1986), suggest that the region
was inhabited by humans probably from 14,000 years before present (BP) and have found there the oldest evidence
of plant domestication in the New World. Since ancient
times, people of this area have used a broad spectrum of
plant and animal species as resources, and cacti have been
among the most important because of their abundance, diversity, and edible parts.
Prehistoric human colonization of the Mexican territory most likely occurred in a north-south direction.
According to this theory, people arrived from northern
Aridoamerica, the vast territory occupied by the Sonoran
and Chihuahuan deserts, where prehistoric bands of
hunter-gatherers interacted for a long time with cacti as
main resources. Inhabitants of the prehistoric Mesoamerica
exhibited a strong cultural utilization of cacti, developed by
their ancestors from Aridoamerica and their own experience with local arid and semiarid environments. Later on,
continual migrations of peoples (including the Aztecs)
from northern Mexico into Mesoamerica progressively reinforced the development of cactus utilization among the
great civilizations; this utilization persists until today.
Mesoamerican Domestication and Diffusion
145
P
P, O
P
P, O
P
P, O
P, O
P
P
P
P
P
P
P
P
Stenocereus stellatus
Adapted from MacNeish (1967) and Smith (1967, 1986).
P
P
P. weberi
P
P
Pachycereus hollianus
P, O
P, O
Opuntia spp.
Myrtillocactus geometrizans
Ferocactus flavovirens
Escontria chiotilla
P, O
P, O
P
P
P
P
Echinocactus platyacanthus
P
P, O
P
P
P
Cephalocereus columna trajani
Venta Salada
(1,300 – 500)
(2,150 – 1,300)
(2,800 – 2,150)
Sta. María
Ajalpan
Purrón
(4,300 – 3,500) (3,500 – 2,800)
(5,400 – 4,300)
Abejas
Coxcatlán
El Riego
Ajuereado
(14,000 – 8,800) (8,800 – 7,000) (7,000 – 5,400)
Palo Blanco
Casas and Barbera
Species
Phase, with radiocarbon date (years before present, BP) in parentheses
TA B L E 9 . 1
Archaeological remains of cacti in caves of the Tehuacán Valley (Puebla, P) and Guilá Naquitz (Oaxaca, O) in central Mexico
146
Smith (1967) reported remains of nine cactus species
(Table 9.1) from archaeological excavations of prehistoric
Mesoamerican sites in caves of the Tehuacán Valley. For
caves at Guilá Naquitz, Smith (1986) reports stems, fruits,
and seeds of Opuntia species in almost all of the stratigraphic zones studied, from nearly 12,000 years BP, as well
as a gumball that could have come from a columnar cactus.
Callen (1967) identified the following types of cactus remains in human coprolites of Tehuacán: (1) “Opuntia,”
which might represent some of the 18 species of this genus
existing in the region (Arias et al. 1997); (2) “Lemaireocereus,” which might represent some of the 13 species of
columnar cacti of the genera Escontria, Myrtillocactus,
Pachycereus, Polaskia, and Stenocereus (Casas et al. 1999a);
and (3) “cactus tissue,” from unidentified cacti. Callen
(1967) further found that in the earliest coprolites from the
El Riego phase (8,500–7,000 years BP), these types of cactus remains were a part of a wild food diet, along with
Setaria spp. seeds, pochote (Ceiba parvifolia) roots, maguey
(Agave spp.) leaves, and meat. In the Coxcatlán phase
(7,000–5,500 years BP), stem tissue and fruits of “Opuntia”
and “Lemaireocereus” were equally dominant materials. In
the Abejas (5,500–3,300 years BP), Ajalpan (3,500–2,900
years BP), Santa María (2,900–2,200 years BP), Palo
Blanco (2,200–1,300 years BP), and Venta Salada (1,300–
460 years BP) phases, findings suggest that consumption of
“Lemaireocereus” stem tissue, fruits, and seeds were more
important than products of “Opuntia”; and during the
Ajalpan and Santa María phases, “Lemaireocereus” was the
principal plant constituent of human diets.Tabe9ln1.eahrer:
The importance of cacti in Mesoamerican cultures can
be recognized in pre-Columbian codices, which contain
many toponymic glyphs referring to the names of cacti or
their parts. Among the most famous are Tenochtitlán
(“place of stony prickly pears” in Náhuatl), the original
name of Mexico City, and Nochistlán (“place of prickly
pears” in Náhuatl), in the state of Oaxaca. Historical information on utilization of cacti can be found in La
Historia General y Natural de las Indias, published by
Oviedo y Valdés in 1535. The Barberini Codex from 1552
(De la Cruz and Badiano 1964) includes information on
medicinal utilization of Tlatocnochtli, a species of Opuntia,
and a description of Teonochtli, identified as Stenocereus sp.
by Bravo-Hollis (1978). The Florentino Codex (Sahagún
1970) contains a section dedicated to the description of the
“diversity of tunas,” which includes a list of variants of
Opuntia species and their uses as edible fruits and stems.
Estrada (1989) identified Cacanochnopalli (a Náhuatl term)
as O. megarhiza, Tecolnochnopalli as O. streptacantha,
Uitzocuitlapalli as Aporocactus flagelliformis, Nopalxochitl as
Epiphyllum ackermanii, Teonochtli as Hylocereus undatus,
Peyotl as Lophophora williamsii (now commonly known as
“peyote”), as well as several types of Tecomitl as Mammillaria, Echinocactus, and Ferocactus species. The Florentino
Codex also includes information on two columnar cacti,
one of them called Netzolli, which is probably Escontria
chiotilla, and Teunochtli, which could be a species of
Stenocereus (Casas et al. 1999a). The books of Francisco
Hernández in the 16th century describe several species of
cacti utilized as medicine, among them several species of
Opuntia, two columnar cacti identified as Myrtillocactus
geometrizans, and a possible Stenocereus species called
Teonochtli (Hernández 1959). The Geographic Relations of
the XVI Century described the cultivation of Opuntia
species for the production of cochineal and contains a reference to the columnar cactus Teonochtli, the “Relation of
Acatlán” (Acuña 1985). Based on these sources, cacti were
clearly utilized as food (fruits, young stems, and in some
cases the flowers and seeds) and medicine (fruits, stems,
and roots).
In the 16th century, Oviedo y Valdés (1535) and
Sahagún (1985) described how the harvest of fruits of
Opuntia spp. and columnar cacti was crucial for subsistence of some pre-Columbian and post-Conquest peoples
from northern and central Mexico. For example, indigenous people migrated during the summer from the coast
of the Gulf of Mexico to the highlands of the northern
plateau, looking for the fruits of platyopuntias. In this region, people stayed for two months, migrating from place
to place consuming fruits.
Smith (1967) considered that species of Opuntia could
have been among the first plants subject to human manipulation in the Tehuacán Valley, but no archaeological
evidence exists. Apparently, Opuntia was cultivated in the
16th century for the production of cochineal (Opuntia
ficus-indica, O. tomentosa var. hernandezii, and Nopalea
cochenillifera). However, cultivation of Opuntia and
columnar cacti for the production of fruits, as currently occurs, was not clearly recorded. The only document that indicates such cultivation is the book of Sahagún (1985), in
which the wild variants are distinguished from others (presumably cultivated). This omission could be because the
Spaniards did not consider fruits of cacti as important resources and therefore did not describe them, as was the
case for many other plants cultivated by natives (Casas et
al. 1999a). Such omission could also be explained if cultivation of these plants started more recently. Further studies can provide information on the changes of cacti under
domestication, which would be helpful to estimate the antiquity of cactus domestication.
Ethnobotanical Information
According to the compendium of the Cactaceae of Mexico
by Bravo-Hollis (1978; Bravo-Hollis and Sánchez-Mejorada
1991), about 850 cactus species occur within the Mexican
territory and 420 in the Mesoamerican region. Ethnobotanical studies in the region have documented a total of
118 cacti species utilized by indigenous peoples (Table 9.2).
Useful cacti include Opuntia species, epiphytes, as well as
columnar, spherical, barrel, and shrubby cacti. Among the
columnar cacti, nearly half of the species are uncultivated
giant columnar cacti, some about 15 m tall, with slow vegetative growth, which flower only after decades (Casas et al.
1999a). However, 23 species of columnar cacti (Table 9.2)
are 2 to 8 m high. They grow faster than the giant columnar cacti, and flower 6 to 8 years after seed germination (2–
4 years after vegetative propagation); most of them reproduce vegetatively and are cultivated.Tabe9l2.neahrere:
Cacti are used mainly for their fruits, which may be
consumed both fresh and dried and are used to prepare
jams (Table 9.2). With the exception of subfamily
Pereskioideae, fruits of nearly all species of cacti are consumed by people (Bravo-Hollis 1978). Fruits of 83 species
(Table 9.2) are the most commonly consumed, and it is
possible to distinguish: (1) species producing sweet juicy
fruits, which are “good quality fruits” and are commonly
harvested; (2) species whose fruits are of “regular quality”
and are collected only occasionally, because of the scarcity
of individual plants or populations, tall branches, long or
abundant spines, or lack of tastiness; and (3) species whose
fruits do not contain juicy pulp and are consumed only
during food scarcity. The main groups of cacti producing
edible fruits are columnar cacti and Hylocereus species with
fruits called pitayas and pitahayas, respectively (Chapter 11);
some Mammillaria species with fruits called chilitos; and,
most important, Opuntia species (Chapter 10), whose
sweet fruits are called tunas and consumed fresh, and
whose sour fruits are called xoconoztles (from the Náhuatl
xocotl, meaning sour, and nochtli, prickly pear) and are utilized as greens, condiments (boiled or fried), or as an ingredient for several other dishes. Colunga (1984) identified
as xoconoztles variants of the species Opuntia joconstle, O.
lasiacantha, O. leucotricha, and O. streptacantha as well as
the red variant jitomatilli of O. megacantha, which is utilized as a substitute for tomato, and the variant brevas of
Opuntia robusta var. robusta, whose peel is consumed fried,
resembling French fried potatoes.
From the useful species of cacti reported, the stems of
62 species are cut and fed (after removal of the spines) as
fodder to domestic donkeys, cows, and goats (Table 9.2).
Mesoamerican Domestication and Diffusion
147
TA B L E 9 . 2
Species of cacti from Mexican Mesoamerica
Species
Acanthocereus pentagonus
A. subinermis
Aporocactus flagelliformis
Backebergia militaris
Cephalocereus apicicephalium
C. chrysacanthus
C. collinsii
C. columna-trajani
C. guerreronis
C. nizandensis
C. palmeri var. sartorianus
C. purpusii
C. quadricentralis
C. senilis
C. totolapensis
Coryphantha radians
C. pallida
Echinocactus platyacanthus
E. cinerascens
E. pulchellus
Escontria chiotilla
Ferocactus flavovirens
F. haematacanthus
F. histrix
F. latispinus
F. macrodiscus
F. recurvus
F. robustus
Heliocereus cinnabarinus
H. elegantissimus
H. speciosus
H. schrankii
Hylocereus ocamponis
H. purpusii
H. stenopterus
H. undatus
a
Usesa
Typeb
Statusc
Mexican statesd
1**, 5, 7, 8
5, 7
8, 9
1, 2
1, 2
1*, 2
1*, 2
1*, 2, 6
1, 2
1, 2
1*, 2
1, 2
1, 2
9
1, 2
2, 5
Shr
Shr
Shr
G col
G col
G col
S col
G col
S col
G col
G col
S col
S col
G col
G col
Sph
w
w, c
c
w
w
w
w
w
w
w
w
w
w
w, c
w
w
7, 16, 19, 21
7, 16
General
9, 15
16
14, 16
16
14
15
16
16
9
16
6, 7
16
4, 5, 6, 16
2, 9
5
Sph
Bar
w
w, m
14, 16
4, 5, 6, 14, 16
1, 8
5
1**, 2, 3, 4, 5, 7, 11
2
1, 2
Shr
Shr
S col
Sph
Sph
w
w
w, m
w
w
4, 5, 6, 10, 11
6, 14
9, 14, 15, 16
14, 16
7, 14
1, 5
1, 2, 5, 9
Bar
Sph
w
w
4, 5, 6, 14
5, 6, 10, 11, 14, 16
1, 5
Sph
w
4, 5, 14, 16
5
Bar
w
14, 16
2
1
1, 8, 9
8, 9
9
1*
Sph
Ep
Ep
Ep
Ep
Ep
w
w, m
w, m, c
w, c
w, c
w
14, 16
21
10, 13, 16
10
6, 14, 16
8, 9
1
Ep
w, c
1, 2, 8, 9, 14, 16
1*
1**, 9
Ep
Ep
w
c
16
General
Reference
Caballero (1992)
Casas et al. (2001)
Bravo-Hollis (1978)
Casas et al. (1999a)
"
"
"
"
"
"
"
"
"
Bravo-Hollis (1978)
Casas et al. (1999a)
Pennington (1963);
Bravo-Hollis (1978)
Casas et al. (2001)
Del Castillo and Trujillo (1991);
Casas et al. (2001)
Sánchez Mejorada (1982)
Casas et al. (2001)
Casas, et al. (1999a)
Casas et al. (2001)
Bravo-Hollis (1978);
Casas et al. (2001)
Del Castillo and Trujillo (1991)
Sánchez-Mejorada (1982);
Casas et al. (2001)
Pennington (1963);
Casas et al. (2001)
Bravo-Hollis (1978);
Casas et al. (2001)
Casas et al. (2001)
Berlin et al. (1973)
Guerra (1986); Cedillo (1990)
Bravo-Hollis (1978)
Casas et al. (2001)
A. Casas (unpublished
observation)
Bravo-Hollis (1978);
Casas et al. (2001)
Bravo-Hollis (1978)
Martínez (1993)
Uses: 1 = edible fruits (*regular quality, **good quality); 2 = fodder; 3 = alcoholic beverage; 4 = edible seeds; 5 = edible stems and flowers;
6 = house construction; 7 = living fences; 8 = medicine; 9 = ornamental; 10 = adhesive; 11 = fuel wood.
b
Type: Op = Opuntia; Ep = epiphyte; Sph = spherical; G col = giant columnar; S col = small columnar; Bar = barrel; Shr = shrubby.
c
Cultural status: w = wild; m = managed in situ; c = cultivated.
d
Numbers for Mexican states (see Figure 9.1) are as follows: 1 = Nayarit; 2 = Jalisco; 3 = San Luis Potosí; 4 = Guanajuato; 5 = Queretaro; 6 =
Hidalgo; 7 = Verzcruz; 8 = Colima; 9 = Michoacán; 10 = México; 11 = Mexico City; 12 = Tlaxcala; 13 = Morelos; 14 = Puebla; 15 = Guerrero;
16 = Oaxaca; 17 = Yucatán; 18 = Chiapas; 19 = Zacatecas.
TA B L E 9 . 2 (continued)
Species
Uses
Type
Status
Mexican states
Lophophora williamsii
8
Sph
w
3, 19
Mammillaria carnea
1, 2
Sph
w
6, 14, 15, 16
9
Sph
w, c
7, 14, 16
5
5, 9
Sph
Sph
w
w, c
7, 14
7, 14, 16
1**
5
5
1*, 2, 6
1**, 2, 3, 5, 7
1**, 2, 3, 5, 7
1, 2, 6
1**, 2, 4, 5, 6
1, 2
1, 2
1**, 2, 4, 5, 6
5, 8, 9
Sph
Sph
Sph
G col
S col
S col
G col
G col
G col
G col
G col
Op
w
w
w
w
w, m
w, m, c
w
w
w
w
w
w, c
7, 9 10, 11, 12, 14
16
16,18
14, 16
9, 15, 16
14, 16
14
9, 13, 14, 15, 16
15
16
14, 16
13, General
N. cochenillifera
2, 5, 8
Op
w, m, c
16, General
N. dejecta
N. escuintlensis
N. lutea
N. karwinskiana
2, 5
1**
1**
8
Op
Op
Op
Op
c
w
w
w
7, 18
18
18
9, 15, 16
Nopalxochia ackermanii
N. conzatitianum
N. macdougallii
N. phyllantoides
Opuntia amyclaea
O. atropes
9
9
9
9
1*, 9
1, 11
Ep
Ep
Ep
Ep
Op
Op
w, c
w, c
w, c
w, c
c
w, m
7, 16
16
18
7, 14
General
4, 9, 10, 13, 15
1**
1**, 11
Op
Op
w
c
9
4, 11
O. decumbens
O. ficus-indica
2, 5, 11
1**, 2, 5, 8, 10, 11
Op
Op
w
c
14, 15, 16
General
O. fuliginosa
O. huajuapensis
O. hyptiacantha
1**, 11
1, 2, 3, 5, 8, 9
1**, 2, 11
Op
Op
Op
w, m
w
w, m
4, 8, 9
14, 16
4, 10, 11
O. imbricata
O. jaliscana
1*, 2, 5, 8
1**, 11
Op
Op
w
w, m
4, 5, 10, 11
4, 9
O. joconostle
O. kleiniae
1**, 11
7
Op
Op
w, m, c
c
4, 5, 9, 10, 11
14
M. collina
M. discolor
M. haageana
M. magnimamma
Melocactus maxonii
M. ruestii
Mitrocereus fulviceps
Myrtillocactus geometrizans
M. schenkii
Neobuxbaumia macrocephala
N. mezcalaensis
N. multiareolata
N. scoparia
N. tetetzo
Nopalea auberi
O. bensonii
O. crassa
Reference
Pennington (1963);
Martínez (1993)
Bravo-Hollis (1978);
Casas et al. (2001)
Bravo-Hollis (1978);
Casas et al. (2001)
Bravo-Hollis (1978)
Pennington (1963);
Casas et al. (2001)
Bravo-Hollis (1978)
"
"
Casas et al. (1999a)
"
"
"
"
"
"
"
Bravo-Hollis (1978);
Casas et al. (2001)
Pennington (1969);
Bravo-Hollis (1978)
Bravo-Hollis (1978)
"
"
Bravo-Hollis (1978);
Martínez (1993)
Bravo-Hollis (1978)
"
"
"
"
Bravo-Hollis (1978);
Colunga (1984)
Bravo-Hollis (1978)
Bravo-Hollis (1978);
Colunga (1984)
Casas et al. (2001)
Bravo-Hollis (1978);
Colunga (1984)
Colunga (1984)
Casas et al. (2001)
Bravo-Hollis (1978);
Colunga (1984)
Sánchez Mejorada (1982)
Bravo-Hollis (1978);
Colunga (1984)
Martínez (1993)
Arias et al. (1997)
(continued on next page)
TA B L E 9 . 2 (continued)
Species
Uses
Type
Status
Mexican states
O. lasiacantha
O. leptocaulis
1*, 2, 9, 11
1, 2, 5, 8
Op
Op
w, m, c
w
4, 10, 11, 14, 16
5, 6, 14, 16
O. leucotricha
1**, 2, 11
Op
w, m
4, 5, 6
O. megacantha
O. nerpicolor
O. pilifera
1**, 11
1*, 5
1*, 2, 11
Op
Op
Op
w, m, c
w
w, m
4
14, 16
1**, 2, 5, 11
Op
w, m, c
4, 5, 6, 9
1**
2, 8
1**, 2, 5, 3, 11
Op
Op
Op
c
w
w, m, c
10
4, 5, 6
4, 5, 6, 14, 16
1*, 5, 8
1**, 2, 5, 11
1**, 11
Op
Op
Op
w
w, m, c
c
16, 18
10, 11
General
O. velutina
Pachycereus grandis
P. hollianus
P. marginatus
P. pecten-aboriginum
P. weberi
Peniocereus serpentinus
Pereskia grandiflora
P. lychnidiflora
1**, 8, 11
1**, 2, 4
1**, 2, 3, 4, 7
1**, 2, 7, 8
1**, 2, 4, 6, 8, 11
1**, 2, 3, 4, 6, 11
9
8
7
Op
G col
S col
S col
G col
G col
Shr
Shr
Shr
w, m
w
w, m, c
w, m, c
w, m
w, m
w, c
c
w, c
13, 14, 15, 16
10, 13, 14
14
10, 14, 16
9, 15, 16
9, 14, 15, 16
14
3
16, 18
Pereskiopsis aquosa
P. rotundifolia
P. velutina
Polaskia chende
P. chichipe
Rhipsalis baccifera
Selenicereus donkelaarii
S. grandiflorus
S. spinulosus
Stenocereus beneckei
S. chacalapensis
S. chrysocarpus
S. eichlamii
S. fricii
S. montanus
S. pruinosus
S. queretaroensis
S. quevedonis
S. stellatus
S. standleyi
S. treleasei
1**, 5, 8
7, 8
7
1**, 2, 4, 7, 11
1**, 2, 4, 7, 11
1**, 2, 3, 4, 6
8
1**, 2, 3, 4, 5, 7
1**, 2, 3, 4, 6
1*, 2, 7
1**, 2
1**, 2
1**
1**, 2, 4, 7
1*
1*, 2, 7, 11
1**, 2, 7
1**, 2, 4, 7
1**, 2, 3, 4, 5, 7, 11
1**, 2, 4, 7
1**, 2, 4, 7, 11
Shr
Shr
Shr
S col
S col
G col
Ep
S col
S col
S col
G col
G col
S col
S col
S col
S col
S col
S col
S col
S col
S col
w, c
w, c
w, c
w, m
w, m, c
w, m
w
c
w, m, c
w
w
w, m
w
w, m, c
w
w, m, c
w, m, c
w, m, c
w, m, c
w, m, c
w, m, c
4, 13
13, 14, 15, 16, 18
5
14, 16
14, 16
9, 13, 15, 16
17
14, 15, 16
14, 15, 16
10, 13, 15
16
9
18
2, 9, 8
2, 8
7, 14, 16
9
9, 15
13, 14, 15, 16,
9, 15
16
O. robusta
O. spinulifera
O. stenopetala
O. streptacantha
O. tehuantepecana
O. tomentosa
O. undulata
Reference
Bravo-Hollis (1978)
Sánchez Mejorada (1982);
Felger and Moser (1983)
Bravo-Hollis (1978);
Colunga (1984)
Colunga (1984)
Sánchez Mejorada (1982)
Bravo-Hollis (1978);
Casas et al. (2001)
Bravo-Hollis (1978);
Colunga (1984)
Bravo-Hollis (1978)
Sánchez Mejorada (1982)
Bravo-Hollis (1978);
Colunga (1984)
Barrera et al. (1976)
Bravo-Hollis (1978)
Bravo-Hollis (1978);
Colunga (1984)
Colunga (1984)
Casas et al. (1999a)
"
"
"
"
Arias et al. (1997)
Alcorn (1984)
Bravo-Hollis (1978);
Martínez (1993)
Bravo-Hollis (1978)
Arias et al. (1997)
Bravo-Hollis (1978)
Casas et al. (1999a)
"
"
Martínez (1993)
Casas et al. (1999a)
"
"
"
"
Bravo-Hollis (1978)
Casas et al. (1999a)
Bravo-Hollis (1978)
Casas et al. (1999a)
"
"
"
"
"
Unfortunately, this list includes long-lived cacti that are
commonly endangered by this utilization. An alcoholic
drink called colonche or nochoctli may be prepared from
fruits of 11 species (Table 9.2). Seeds of 17 species are consumed by people (Table 9.2). In general, seeds obtained
from fresh or dried fruits are washed, dried, and roasted to
prepare traditional sauces or ground into an edible paste
that is consumed with maize tortillas.
Stems and sometimes flowers of 34 species are consumed by humans (Table 9.2). Stems of Opuntia spp. and
columnar cacti have long been a common food (Callen
1967). At present, consuming young stems of Opuntia and
Acanthocereus as vegetables and utilizing the stems of barrel cacti to prepare candies are locally common, but the
young stems of columnar cacti are eaten only during food
scarcity. Undoubtedly, cladodes of platyopuntias are by far
the most appreciated plants for stem consumption, although many species may be consumed; among the preferred and even commercialized are variants of O. atropes,
O. fuliginosa, O. hyptiacantha, O. jaliscana, O. joconostle,
O. megacantha, O. streptacantha, O. tomentosa, O. velutina,
and, of course, O. ficus-indica (Colunga 1984). Flower buds
are commonly consumed after boiling, e.g., Pereskiopsis
species, whose leaves are also consumed as greens.
Wood of 9 species of columnar cacti is used in construction of house roofs and fences, and 22 species of
Opuntia, Pereskia, Pereskiopsis, and columnar cacti, among
others, are grown as living fences and/or as barriers for soil
protection in terraces of cultivated slopes (Table 9.2). A
total of 22 species are utilized as medicine. Among the
main diseases traditionally treated with cacti are stomach
ache, gastric ulcers, rheumatism, dysentery, diabetes, obesity, and heart diseases (Chapter 13), and some species are
used as analgesic, anti-inflammatory, or diuretic agents
(Table 9.2).
Also appreciated for their beauty, cacti have been collected, cultivated, and some domesticated as ornamental
plants in Mexico and other parts of the world (Chapter 8).
For the scope of this chapter, only the 19 species considered
as ornamental in rural Mexico are so counted (Table 9.2).
The mucilage of O. ficus-indica and Pachycereus hollianus is
utilized as an adhesive (Chapter 13). The dry stems of 25
species are utilized as a fuel for heating and to prepare food,
and the stems of Polaskia chichipe, P. chende, and
Stenocereus stellatus are sources of fuel wood for manufacturing pottery.
Peoples of Mexican Mesoamerica gather fruits and
other useful products of cacti from wild populations
(Table 9.2). In general, they gather fruits selectively, preferring larger fruits of species or variants with juicy pulp,
sweeter or, for xoconoztles, sourer pulp, or thinner edible
pericarp (thicker for xoconoztles), shorter and fewer spines,
and deciduous areoles (Casas et al. 1997a). Similarly, for
collecting edible stems, people take into account the
thickness of stems and prefer fewer spines, less mucilage,
lack of a bitter flavor, and no fibrous texture (Colunga et
al. 1986). When they clear the vegetation for cultivating
maize, peasants frequently tolerate or let stand individuals
of 19 species of columnar cacti, variants of 13 species of
Opuntia, and 5 species of other cacti (Table 9.2). They
commonly plant vegetative propagules of the spared cacti,
enhancing their local abundance. Because these compete
with cultivated plants, people carefully select the individuals to spare. Their decisions take into account the usefulness and desirable characteristics of the species and individuals in terms of the products that they produce. About
46 species are cultivated by planting vegetative parts in
home gardens or in agricultural plots where they serve as
living fences or as barriers to prevent soil erosion (Table
9.2). Vigorous branches or cladodes from wild or cultivated individuals are irrigated, and ash is commonly used as
a fertilizer. Individuals cultivated in home gardens may also
be derived from seedlings established from seeds dispersed
via bird, bat, or human feces. Because people often do not
recognize variants of cactus species based on vegetative
characteristics, decisions on eliminating or sparing individuals are made when the individuals first produce fruits.
Domestication of Cacti
Columnar Cacti
Although 12 species of columnar cacti are cultivated (Table
9.2), the only cases studied under controlled domestication
process are Stenocereus pruinosus (Luna 1999), S. queretaroensis (Pimienta-Barrios and Nobel 1994), and S. stellatus (Casas et al. 1997a, 1999b,c). Stenocereus pruinosus occurs
in southeast-central Mexico, S. queretaroensis in westcentral Mexico, and S. stellatus is endemic to south-central
Mexico (Fig. 9.2). These species occur in the wild in tropical deciduous and thorn-scrub forests, but some wild populations are managed in situ and all three species are cultivated (Casas et al. 1999a). They exhibit considerable
morphological variation, especially in fruit characteristics,
which is presumably partly under genetic control, partly
influenced by environmental conditions, and partly the result of human manipulation.Fgiure92.neahrere:
According to archaeological information from caves in
Tehuacán, S. stellatus has been used for nearly 5,000 years
(MacNeish 1967; Smith 1967). Current indigenous groups
use and manage this species and S. pruinosus mainly for
Mesoamerican Domestication and Diffusion
151
Figure 9.2. Range of Stenocereus pruinosus, S. queretaroensis, and S. stellatus (Pimienta-Barrios and Nobel 1994; Casas et al. 1997a; Luna 1999).
their edible fruits. Management in situ of wild populations
of S. pruinosus and S. stellatus is carried out by keeping desirable phenotypes while removing others, and by cutting
and planting branches of desirable phenotypes (Casas et al.
1997a, 1999a). Cultivation is practiced mainly in home gardens, where desirable phenotypes are vegetatively propagated and new variation is incorporated by volunteer
seedlings. Similarly, relictual populations of S. queretaroensis, associated with pre-Columbian settlements, have
been continuously used for long periods in southern
Jalisco and Colima (Benz et al. 1997). This species is now
widely cultivated in Jalisco and Guanajuato, most of this
domestication apparently stemming from the 19th century (Pimienta-Barrios and Nobel 1994).
Pulp color, flavor, amount of edible matter, skin thickness, and spinyness of the mature fruits are the most
significant characteristics used in folk classification of
152
Casas and Barbera
variants, assessing quality of products, and selecting individuals of these columnar cacti for propagation (PimientaBarrios and Nobel 1994; Casas et al. 1997a, 1999a,b; Luna
1999). Manipulation of these species involves artificial selection. This is particularly intense in home gardens,
where manipulation is accomplished by continually planting and replacing individuals, but also is significant in
managed populations in situ, where selection mainly increases frequencies of favorable phenotypes in wild populations (Casas et al. 1997a).
Where artificial selection has been significant, both
management in situ and cultivation of S. stellatus may
change morphology from that in wild populations, especially for those characters that are targets of human selection (Casas et al. 1997a, 1999a). Morphology was therefore
compared among individuals from populations under
different management regimes. Populations were sampled
1.0
principal component 2
0.5
0.0
-0.5
-1.0
-1.0
-0.5
0.0
0.5
1.0
principal component i
Figure 9.3. Principal component analysis of morphological variation for Stenocereus stellatus in the Tehuacán Valley, Puebla (w = wild; m =
managed in situ; c = cultivated individuals; modified from Casas et al. 1999a). Characters analyzed include numbers and dimensions of fruits
(and their parts), seeds, spines, and branches.
from the Tehuacán Valley (Fig. 9.3) and La Mixteca Baja to
examine to what extent morphological variation in populations can be related to environmental factors.Fgiure93n.eahrere:
Principal component analysis indicates morphological
similarities among populations according to their management regime. Most wild individuals in the Tehuacán
Valley occupy the left side of the figure, most cultivated
ones are on the right side, and those from managed in situ
populations predominate in the middle (Fig. 9.3). The
most significant characters are fruit size, amount of pulp,
and seed weight (positive values) and density of spines and
skin thickness on fruits (negative values). Cultivated individuals have the largest and least spiny fruits, the thinnest
peel, and the heaviest seeds. The number and dimensions
of vegetative parts, fruits, and seeds are higher in La
Mixteca, whereas the density of spines on the fruits is higher in the Tehuacán Valley. More intensive management
leads to larger fruits with a higher proportion of pulp,
more and heavier seeds, fewer spines per unit stem area,
thinner fruit skin, and a higher proportion of individuals
that produce fruits with a green skin and sweet pulp with
a color other than red.
Thus, human management has influenced morphological divergence of both managed in situ and cultivated
populations from wild populations of various species, so
domestication may be caused not only by cultivation but
also by management of wild populations (Casas et al.
1997a). Although the phenotypes of managed in situ and
cultivated populations originate from wild populations,
some cultivated phenotypes are rare or have not been observed in the wild. This is especially the case for individuals with large fruits and pulp colors other than red. Only
2.3% of the individuals sampled in wild populations have
pink or yellow pulp, and other pulp colors (purple, orange, and white) are not observed in the wild (Casas et al.
1999b). On the other hand, 42% of individuals sampled
in cultivated populations at La Mixteca have these phenotypes. Success of such phenotypes is low in the wild,
and only under human protection (i.e., domestication of
S. stellatus) are individuals with favorable characteristics
Mesoamerican Domestication and Diffusion
153
that are scarce or absent in the wild protected and
enhanced.
Variation in S. stellatus is influenced by environmental
conditions, genetic differentiation, and other factors. The
clearest environmental difference between the two regions
is annual precipitation, higher in La Mixteca (average of
740 mm) than in the Tehuacán Valley (510 mm). In all populations, anthesis of S. stellatus is predominantly nocturnal,
and bats are the most probable pollinators (Casas et al.
1999c). With bats as pollinators, movement of pollen between populations is expected, as bats can commute 30 km
from their roosts (Sahley et al. 1993). Isolation by distance
within regions is therefore unlikely between wild, managed
in situ, and cultivated populations, because distances separating these populations are generally less than 10 km. In
addition, flowering in wild and cultivated populations overlap by at least 75 days, indicating that temporal barriers for
pollination between populations are also unlikely.
Stenocereus stellatus is out-crossing, and major differences exist between wild and cultivated populations.
However, experimental crosses indicate pollen incompatibility between certain cultivated phenotypes, especially
those with the greatest domestication. This can partly explain the morphological and genetic divergence among
wild, managed, and cultivated populations. Nevertheless,
the absence in wild populations of the phenotypes typical
of home gardens may also be explained by failures in seed
germination or in the establishment of these variants
under wild conditions. Similar to results with S. stellatus,
Luna (1999) analyzed the morphology of wild, managed in
situ, and cultivated trees of S. pruinosus, finding significant
differences in fruit mean weight: wild, 38 g; managed in
situ, 70 g; and cultivated, 188 g. Seeds are larger and more
numerous in cultivated variants. For S. queretaroensis,
weights of fruit from wild trees (60–90 g) are higher than
those from cultivated trees (over 130 g; Pimienta-Barrios
and Nobel 1994). For S. fricii in Michoacán, fruit weight
in wild populations is extremely variable but averages 130
g, whereas fruits of the cultivated variants average 230 g
(Rebollar et al. 1997).
Opuntia Species
Opuntia ficus-indica, O. megacantha, O. streptacantha, O.
robusta var. larreyi, and O. joconostle are the most commonly cultivated opuntias (Bravo-Hollis 1978). Yet in the
El Bajío region of Guanajuato, Mexico, 16 Opuntia species
are used for their edible products: young cladodes (nopalitos) and fruits (Colunga et al. 1986). The edible products
of Opuntia are very important in the local peasant economy, because they are particularly abundant before the har-
154
Casas and Barbera
vest season of maize and sometimes the latter is scarce;
their commercialization determines about 20% of the annual income of local people (Colunga 1984). Interactions
include gathering, tolerance, and enhancement in situ in
areas cleared for agriculture, and cultivation in agricultural plots and home gardens.
The different utilization and management of Opuntia
species and particular populations is due to the recognition
of specific attributes and morphological differences among
species and variants (Colunga 1984). Local people recognize nearly 70 variants belonging to the 16 species, which
are broadly classified as ‘manso’ (docile) and ‘de monte’
(from the mountains). The ‘manso’ variants include plants
whose edible products are of better quality but are dependent on humans for survival. These presumably domesticated variants include 17 of Opuntia crassa, O. ficusindica, O. megacantha, O. robusta var. larreyi, and O.
undulata. The ‘de monte’ variants do not depend on humans for survival and are presumably wild or weedy variants. Within the ‘de monte’ variants are plants producing
fruits with thick sour edible peel named xoconoztles (including variants of Opuntia joconostle and two variants of
O. lasciacantha), others producing fruits with thin peel and
sour pulp called jocotunas that are consumed entire as
greens (including variants of Opuntia aff. leucotricha and
one variant of O. streptacantha), and others producing
fruits with thin peel and sweet pulp called tunas. Among
the characteristics for classifying and selecting phenotypes
for differential management of Opuntia variants are fruit
flavor, color, peel thickness and hardness, form, size, and
spinyness as well as cladode size.
Colunga (1984) performed multivariate statistical
analyses with 9 individuals of each of 70 variants of 16
species and considered 69 morphological characters to explore patterns of morphological similarity among the variants. These analyses consistently separated the ‘manso’ and
the ‘de monte’ variants. Nearly 34 ‘de monte’ variants of the
species O. atropes, O. fuliginosa, O. hyptiacantha, O. jaliscana, O. joconostle, O. lasiacantha, O. megacantha, O. streptacantha, O. tomentosa, and O. velutina are managed in situ
and are morphologically intermediate between the ‘manso’
and the ‘de monte’ variants. Thus, artificial selection apparently causes a morphological divergence not only between the wild and domesticated variants, but also between the wild and the managed in situ variants. The
characters with higher significance in defining these groups
relate to the dimensions of fruits and cladodes, the frequency of areoles on fruits and cladodes, fruit and seed
form, and seed number and weight (Colunga et al. 1986).
Of the species studied by Colunga (1984), O. megacantha
A
B
C
Figure 9.4. Various early depictions of opuntias: (A) the first opuntia depicted by a European illustrator (Oviedo y Valdés 1535); (B) drawing of
an opuntia in Mattioli’s 1558 edition of De Materia Medica; and (C) drawing of an opuntia in Mattioli’s 1568 edition of De Materia Medica.
is the only taxon having wild, managed in situ, and domesticated/cultivated variants; based upon morphological
data, the three types clearly form discrete groups according
to their management (Colunga et al. 1986), as for S. stellatus (Fig. 9.3). Fruit, cladode, and seed dimensions are the
most significant characters for this classification, and the
management regime thus apparently plays a role in domestication of cacti.
Diffusion of Cacti to Other Regions of the World
Hundreds of species of cacti are presently part of plant collections in botanical gardens and some have become popular as ornamental plants worldwide. The “dragon fruit”
Hylocereus undatus is an important crop in southeastern Asia
after its introduction to the Philippines by Spaniards in the
16th century; trials are being conducted in Israel and the
United States for cultivating it along with some of the fruitproducing columnar cacti (Chapter 11). But, undoubtedly,
platyopuntias are the cacti most widespread out of their
original range (Chapter 10), their dissemination having been
initiated by humans after the conquest of the New World by
Europeans. The first European to report on American
platyopuntias was the Spanish state officer Oviedo y Valdés
(1526), who wrote about two prickly pear cacti common on
the island Hispaniola (now Haiti). In a later work (Oviedo
y Valdés 1535), these plants were described in greater detail,
one of them being depicted for the first time in the drawing of a European illustrator (Fig. 9.4A).Fgiure94.neahrere:
The wonder of the plants of the New World (“so beautiful and so different from ours,” as Columbus wrote on his
first pages about America) turned into bewilderment over
the strangeness. The flora of the Old World did not include plants that were morphologically similar to platyopuntias, therefore Oviedo y Valdéz could not tell whether
“this is a tree or rather a monster amidst trees,” and he
wrote that great painters, such as Leonardo da Vinci and
Andrea Mantegna, would be necessary to describe it properly. His incredulity was shared by other European travelers, who in the subsequent 50 years talked about the plant,
struck by some of its amazing features: leaves growing one
upon the other into such a tree that it was impossible to
find something “wilder or uglier” in the plant kingdom
(Oviedo y Valdés 1526) and fruits that turned the urine red,
which aroused great fear and became a prank played on
newcomers. The fruits, called tunas by Oviedo y Valdés
(1535), were commonly sold in markets; they were so delicious that his fellows “knew and ate [them] . . . with pleasure” and they were “as lovely as figs.” The name given to
the best-known species, Opuntia ficus-indica, reflects this
supposed morphological resemblance and the geographical origin (the West Indies). The fruits of other opuntias
mentioned by Oviedo y Valdés were mashed, dried, and
used as dyestuff, while their cladodes were used in compresses to treat bone fractures, probably because of the
anti-inflammatory action of mucilage (the “bone-fixing
tree”).
Mesoamerican Domestication and Diffusion
155
156
Colonial Period
Spread of Platyopuntias in Europe
When the Spanish conquistadors landed in Mexico,
Oviedo y Valdés’s curiosity was renewed and enhanced by
the symbolic role that opuntias played in the complex Aztec
cultural world. However, the interest in the commercial exploitation of the plant and especially in its fruits (offered as
a present to the invaders in 1519 during Hernando Cortés’s
march on the Aztec capital [Diaz del Castillo 1991])did not
go beyond a vague and cautious consideration, similar to
that shown for many of the foods eaten by the American
natives. Although Europeans liked its fruits as much or
more than the local populations (Toribio de Motolinía 1541
and López de Gomara 1552, cited in Donkin 1977), the
prickly pear was not introduced to Europe for food but as
an ornamental plant. This was also the case for most
American species that only later became staple crops and
foods throughout Europe. One of the tasks of European
travelers was to collect new and curious-looking plants, and
prickly pears undoubtedly fell into that category.
Despite the lack of records, prickly pears are thought
to have been first introduced to Europe in western
Andalucía, probably in Seville. Beginning in 1495, this city
had become the center of trade with the Americas through
its Casa de Contratación; its numerous garden plants were
acclimatized and studied for their medicinal properties
(Valdes et al. 1992). Because of its importance to the
Mesoamerican civilization, the first plant to be brought to
Spain was probably Opuntia ficus-indica. This species,
which was likely the one described by López de Gomara in
1552 (taking for granted that it was already known in
Spain), seems to be represented in the first illustration of
a specimen grown in Europe, published in the 1558 edition
of De Materia Medica by the Italian physician Mattioli
(Fig. 9.4B). A product of the plant, indirectly obtained by
the insect that feeds on the plant, was apparently known
and appreciated by the Spaniards more than the plant itself—the red dye, called grana cochinilla (cochineal). The
conquistadors were impressed by the wonderful colors of
Aztec artifacts, and probably as early as 1520—although the
first record dates back only to 1543—small quantities of the
dyestuff were brought to Spain. The new product met with
instant success in European markets, because its dyeing
power was ten times greater than kermes, the coloring matter used in the Old World and previously considered the
best of red dyes. In the last quarter of the 16th century,
Spain annually imported 70,000 to 90,000 kg of grana cochinilla, and this substance became one of the the most
sought-after commodities from the American continent,
preceded only by precious metals (Donkin 1977).
In the second half of the 16th century, prickly pears began
to spread rapidly, first in many botanical gardens all over
Europe. In the meantime, more knowledge about the plant
arrived from America: Francisco Hernández from 1571 to
1576 and Sahagún in 1570 described several varieties,
Cervantes de Salazar in 1554 pointed out that cladodes
could easily take root, and G. Gómez de Cervantes in 1599
provided the first hints on growing techniques (Donkin
1977; Alvarez López 1946). Among European countries, Italy
was particularly attracted to the new American plant—it
was the Renaissance, arts and sciences were flourishing,
and a deep interest in plants coming from the New World
developed. Nonetheless, the prickly pear was always looked
upon with suspicion. Galeotto Cei (1991) considered it so
“malignant” that “however appreciated it may be in Italy,
I would not wish to see or find it anywhere.” A physician
from Siena, Pier Andrea Mattioli, had a different attitude:
he regarded the plant as “one of the wonders of nature,”
and his interest grew constantly in the various editions of
his famous book (Mattioli 1558, 1568). No mention of
prickly pears is made in the first issue of 1544 (Donkin
1977), whereas in 1558 the plant is depicted by a drawing,
and two more illustrations are present in the 1568 version
(Fig. 9.4C). The curious appearance of the plant, which in
1580 Soderini associated with the turkey to show the peculiarity of American nature (Tangiorgi Tomasi et al.
1990), helped it spread from botanical gardens to aristocratic estates all over Europe. In Italy, prickly pears were
grown in Florence (Mattioli 1568). Dodonaeus in 1583 reported their dissemination into Germany and Holland and
Gerard in 1596 into England (Alvarez López 1946).
More evidence of this popularity is provided by botanical drawings and engravings representing the plant, and by
its appearance in the figurative arts. Platyopuntias are depicted in a painting by Bruegel the Elder (“Land of Plenty,”
1567) as well as in some of the most beautiful botanical illustrations of the Renaissance, such as the watercolor of
Iconographia Plantarum by Ulisse Aldovrandi (end of the
16th century; Baldini 1990) and the tables of Hortus
Eystehensis (1600). The plant was highly rated and is considered necessary in a royal garden (Agostino del Riccio
[undated but end of the 16th century], cited in Tangiorgi
Tomasi et al. 1990), or thought to have occurred in Eden,
as one of Switzer’s 17th century xylographies shows.
Casas and Barbera
History of Name
In the 17th century, prickly pears were “grown in the roof
gardens of noblemen’s mansions” (Bahuin 1650–1651, cited
in Alvarez López 1946). Being closely linked to the botanical tradition of ancient Greece, Mattioli (1568) deemed
impossible the acceptance of a new species and traced the
newly discovered plants back to the flora of the classical
world. In Commentarii in sex Libros Pedacii Dioscoridis, the
prickly pear is mentioned in a chapter about the
Mediterranean fig (Ficus carica). Two Indian figs are described: a large tree with branches that root, identified with
the same plant mentioned by classical writers such as
Theophrastus, Strabo, and Pliny; and another Indian fig,
introduced from the West Indies “in our time,” the fruits
of which are called tune (tuna) by Indians. Mattioli believed the latter to be Opuntia plinii, the plant already described by the great Latin agronomist and named after
Opunte, a town in Locrid (Greece), where grew a plant the
leaves of which could root.
The fact that an American plant could be known to
Greeks and Romans did not seem absurd to Mattioli and
to many other European botanists after him. Some did
raise doubts, such as the Spaniard Laguna in 1563, who
“dared not affirm” that the prickly pear was Pliny’s plant.
Others did not seem to see the contradiction: in fact,
Fragoso in 1572 and Rouvillium in 1587 believed it to be an
American plant, but claimed it was already known by the
ancients (Alvarez López 1946). Although Parkinson in 1619
stated with certainty that this could not be the plant described by Pliny, others persisted in believing the fallacy for
a long time, essentially based on the observation that
cladodes take root (Alvarez López 1946). The occurrence of
the prickly pear in natural and cultural landscapes of
Mediterranean Europe is so common, popular texts still
say that this plant is a native species, or that it was introduced from the East Indies by the Arabs, as Gerard
claimed in 1633. Considering that the various botanical
names contain a semantic error, either based on the appearance of the fruits or on the geographical origin (Cactus
ficus-indica Linnaeus, 1753; Opuntia ficus-indica [L.] Miller,
1768; Cactus opuntiae, Gussone, 1827–8; Opuntia ficusbarbarica Berger, 1912), one can perfectly agree with de
Candolle (1883) when he said that “everything about this
name is false and ridiculous!”
Naturalization in Europe
In northern Europe, platyopuntias cannot survive winter
outside the privileged space of botanical gardens. In milder
Mediterranean areas, the plants have found optimal environmental conditions, spreading and naturalizing so as to
become one of the typical features of Mediterranean landscapes (Barbera et al. 1992). Prickly pear cacti can grow in
places where the average minimal temperature does not go
below 1 to 2°C, and average relative humidity does not remain lower than 40% for more than 1 month. In regions
where the plants are common and commercially exploited,
annual rainfall ranges from 400 to 600 mm, but they can
also grow in areas with only 200 mm or up to 800 mm annually (in the latter case, the lithologic substrate must favor
soil drainage). Under these conditions, platyopuntias grow
independently of the lithologic matrix, even if they are typically found on volcanic soils and calcareous rocks, where
they occur in scrub patches together with Euphorbia dendroides, Artemisia arborescens, Calycotome infesta, and other
species (La Mantia et al. 2001). According to Le Houerou
(1996), the major occurrence of platyopuntias in the western Mediterranean is due to a shorter and less severe dry
season, which favors the natural spread of the species, in
comparison with drier eastern regions. In addition to O.
ficus-indica, other naturalized species growing on Mediterrean coasts are O. decumana, O. dillenii, O. stricta, O. vulgaris, O. amyclaea, and O. robusta, the last two of which inhabit frost-free areas only.
Dissemination of species was clearly encouraged by
their widespread use in Mediterranean farming. After
southern Spain, the first regions that knew and valued
prickly pear were North Africa and Sicily, because of geographic contiguity or social and political relationships with
the Spanish peninsula. Soon platyopuntias spread all over
the Mediterranean coast and, taken aboard ship to prevent
scurvy, followed European colonization and reached the
remaining parts of Africa, Asia, and Australasia. In the
Mediterranean, prickly pears turned out to be an inexhaustible source of products, first as a subsistence crop and
later as a cash crop. The local populations immediately appreciated the new species, but neither cochineal production nor human consumption of young cladodes (nopalitos; both common in Mesoamerica) gained ground. Some
attempts at cochineal culture were made, but only in the
19th century in Malta (1828), Algeria (1834), and Sicily
(1860). The Spaniards, who held the monopoly of the
cochineal industry, banned the export of insects until 1777,
when the botanist N. J. Thierry de Menonville managed to
introduce them to French dominions. Attempts at raising
cochineal insects feeding on O. ficus-indica or Nopalea
cochenillifera failed in the Mediterranean area, because
these coccids cannot bear the combination of excessively
low temperatures and frequent winter rainfall. The
cochineal industry proved successful only in the Canary
Islands, where it became a profitable economic activity, especially on the island of Lanzarote, and remains so since its
introduction in 1826 to 1835 (Donkin 1977; Baranyovits
1978). On the other hand, the use of nopalitos for food has
Mesoamerican Domestication and Diffusion
157
remained almost unknown in Europe; the only food habit
that bears some resemblance to its use in Mesoamerica is
found in some areas of inner Sicily, where the fruit skin is
breaded and fried.
Platyopuntias in Sicily
In Sicily, O. ficus-indica became very popular and acquired
considerable economic significance for its fruits. They became an important staple crop, but were also considered as
a forage crop in extensively farmed inner regions. Platyopuntias were grown to ensure forage in times of emergency
and were planted near rural buildings and animal shelters
to form enclosures or dense scrub. They originally occurred
as fruit plants in “pleasant gardens,” as Bonanno reported
in the 17th century, but their cultivation soon spread
(Coppoler 1827), both in inner areas and on the coast,
where they were to be found in “dry” orchards together
with vines and olive, almond, carob, and pistachio trees.
The fruit soon entered local markets: a platyopuntia is depicted on what seems to be a market stall in a still life by an
unknown Sicilian painter who lived around 1640 and was
named “maestro del ficodindia” (master of the prickly pear)
because of this work (Barbera and Inglese 1993).
Platyopuntias had a leading role in the food habits of
Sicilians. They were described as “the bread of the poor,”
and De Gasparin, a French agronomist who visited the island around 1840, said they were “the manna, the blessing
of Sicily, the equivalent of the banana tree to equinoctial
countries or the breadfruit tree to the Pacific Islands”
(Biuso Varvaro 1895). The fruits can be eaten fresh, or are
sun-dried and stored for the winter, following a peasant
tradition. The juice, concentrated through boiling and seasoned with flour and spices, is used in recipes for special
cakes called ‘mostaccioli,’ or for chutneys. The fruits were
also used for ethanol production; in 1865 a plant in
Catania obtained 2,500 liters per day and around 1940, in
search of energy sources to ensure economic self-sufficiency
to the country, this opportunity was again considered, and
platyopuntias were extolled. Among other Sicilian rural
traditions linked to this cactus are the production of a red
dye obtained from the fruits of O. dillenii and the use of a
decoction of dried flowers for diuretic purposes, a natural
remedy that is still widely used and justified by the presence of a glucosidic flavonoid (isoramnethin) and a high
potassium content. In Spain, the flowers were used to treat
seizures in children in the 18th century. Convex cladodes
served as plates in picnics, or as containers for the manna
extruding from the tapped trunk of Fraxinus angustifolia.
Sicilian farmers also use prickly pears for soil conservation,
as mulch, and as windbreak hedges (Barbera 1994).
158
Casas and Barbera
Fresh fruit production became the most important
commercial exploitation, particularly beginning in the 19th
century. In coastal areas, especially near the main towns,
production flourished for local and export markets
(Genoa, Marseille, London, and, from the 1850s, New
York). Commercial success was favored by a cultivation
technique called scozzolatura, whereby in autumn fruits of
better quality and preservability than summer produce
were obtained and put on the market when competition
was less. The origins of this technique reflect the culture of
the archaic Sicilian rural world. The agronomic chronicle
by Alfonso Spagna (1884) gives an account of a quarrel
breaking out, in a town not far from Palermo, between a
farmer, who did not want to sell his product, and a merchant, who took his revenge by knocking down the fruits
in full bloom. In Ventimiglia Sicula (famous for its delicious fruits that were sold in Palermo), a dispute arose between a son, who in May 1819 performed thinning to have
bigger fruits, and a father who, ignoring the beneficial
effect of this technique, removed all the fruits from the
plant. In both cases and against all expectations, the
plants blossomed again, and the late-ripening fruits were of
higher quality.
Expansion to Other Mediterranean Regions
In Sicily, spineless forms were widely used for forage in the
1920s, when they were replaced—albeit keeping a minor
role—by other crops that were thought to be more suitable
for the climatic conditions of the region. From 1920 to
1930, the plant enjoyed great success in drier North African
areas, being widely grown both in specialized farms as a
forage crop and in extensive areas for erosion control, land
reclamation, and rehabilitation. Platyopuntias were so
important that Monjauze and Le Houerou (1965) regarded their dissemination as the “pre-arboricultural stage of
farming,” and described them as the plants that made possible the shift from nomadism to agriculture. Today in
North Africa, especially in southern Tunisia, the vigorous
action taken by programs against desertification, soil erosion, and dune movement, combined with the production
of fodder for livestock, envisages the use of platyopuntias
together with Acacia and Atriplex species (Chapter 12). To
encourage forage production, much research was carried
out in Tunisia and Algeria on cold-hardy species and clones
of O. ficus-indica, many of which were brought from
Mexico to be grown in arid highlands (Le Houérou 1996).
Platyopuntias are now common in arid regions that are
subject to water and wind erosion. They prove effective in
preventing soil loss, accumulating wind borne deposits,
and reducing land degradation. They are also used to slow
and direct sand movement, enhance the restoration of vegetative cover, stabilize the soil, and prevent water from destroying land terraces built to reduce runoff. The popularity of platyopuntias in North Africa is demonstrated by
extensive cultivation areas begun in the 1950s, ranging
from 700,000 to 1,000,000 hectares in Tunisia, Algeria,
and Morocco (Nefzaoui and Ben Salem 2000).
Platyopuntias currently play a minor role in other
Mediterranean countries. In Spain, Greece, and Turkey,
they frequently occur in home orchards or vegetable gardens for human and animal consumption (fruits are particularly appreciated by pigs), but it is not common for
commercial exploitation. They are also popular in Israel,
where in the Negev Desert specialized farms covering hundreds of hectares can obtain two crops per year. The fruit
of platyopuntias is called sabra in Israel, the same term
used for people native to the country (because their character is like the fruit, spiny outside but sweet inside).
Further indicating the exotic origin of the species are other
popular names: e.g., the Arabs call it “Christian fig” and
the French “Barbary fig” (from the ancient name of North
Africa).
Conclusions and Future Prospects
Although a broad spectrum of cacti could have been crucial for sustenance of Mesoamerican people for a long
time, only a few species have been recorded in archaeological excavations, and uncertainty exists about forms of
utilization and management. The conjunction of archaeological and botanical research may clarify these aspects. In
particular, Mesoamerican people currently utilize and
manage many species of cacti. People presently decide how
to manipulate cacti according to the quality of their products and their roles in human subsistence. The species and
varieties cultivated or managed in situ are generally those
with the most useful fruit characteristics. As found for
Stenocereus stellatus (Casas et al. 1997a), cultivation is particularly intensive where the commercialization of fruits or
their consumption by households leads to more and/or
better fruits. Availability of plant resources is another crucial factor influencing their management; e.g., S. stellatus
is intensively cultivated in places where wild populations
are scarce, but not where they are abundant. Although
species such as Mitrocereus fulviceps, Neobuxbaumia tetetzo,
N. mezcalaensis, and Pachycereus weberi produce good
quality fruits, and species of barrel cacti produce economically important stems (Table 9.2), they are not cultivated
ex situ, because their slow growth makes the effort of sowing seeds and taking care of seedlings unrewarding for
decades. Slow growth may not be relevant for decisions on
managing wild populations of such species in situ.
However, artificial selection favoring particular phenotypes
in situ may reflect difficulties in increasing the frequency of
desirable phenotypes by intentional direct propagation.
Furthermore, when the seeds of desirable phenotypes are
sown, the additive genetic variance of desirable traits can
make it uncertain that the phenotypes selected are those
expressed in the progeny. In contrast, the fixation of desirable characters in species with vegetative propagation,
such as S. pruinosus, S. queretaroensis, or S. stellatus and
Opuntia species, is relatively easy.
Artificial selection is carried out by identifying and subsequently increasing by vegetative propagation individuals
that have desired phenotypes from wild, managed in situ,
or cultivated populations. Artificial selection is also applied
when plants of desired forms are preferentially spared or
protected when land is cleared, or when seedlings are spared
in cultivated populations until their fruits can be evaluated. Artificial selection has achieved significant results for
species such as Stenocereus fricii, S. griseus, S. pruinosus, S.
queretaroensis, and S. stellatus (Pimienta-Barrios and Nobel
1994; Casas et al. 1997a, 1999a, b; Rebollar et al. 1997), as
well as for Opuntia crassa, O. ficus-indica, O. megacantha,
O. robusta var. larreyi, O. undulata, and probably O. joconostle (Colunga 1984; Bravo-Hollis 1978). Artificial selection can also be significant for Escontria chiotilla,
Myrtillocactus geometrizans, M. schenckii, Pachycereus hollianus, P. marginatus, Polaskia chichipe, and P. chende (Casas
et al. 1999a) and variants of Opuntia atropes, O. fuliginosa,
O. hyptiacantha, O. jaliscana, O. joconostle, O. lasiacantha,
O. megacantha, O. tomentosa, O. streptacantha, and O. velutina (Colunga, 1984), which are intensely cultivated
and/or managed in situ and which exhibit morphological
variation in characters that are targets of human preference.
The developing case studies of Opuntia and Stenocereus
species provide a model of in situ and ex situ artificial selection that can help analyze patterns of domestication and
can lead to hypotheses for testing in future research.
Ornamental cacti, Hylocereus undatus, columnar cacti,
and Opuntia species are the main groups of the family
Cactaceae diffused by humans in a process lasting about
500 years. Apart from Opuntia, particular forms of utilization and selection of these cacti should be documented in their new environments and under new human cultural conditions. The consequences of artificial selection in
the evolution of these species needs to be evaluated.
Particularly interesting will be a comparison in morphological and genetic changes developed during these 500
years in the context of human cultures and environments
of the New and Old Worlds.
Mesoamerican Domestication and Diffusion
159
In any case, platyopuntias have become an integral part
of the Mediterranean landscape and agricultural economy.
To further develop its cultivation requires higher-quality
and glochid-free fruits. Today, consumption is mostly
confined to immigrants of rural origin, who are already familiar with the fruit in their homelands. In countries such
as Belgium, France, Germany, and Great Britain, demand
comes mainly from migrant workers from Italy and Africa.
The market would expand considerably by attracting a
different category of consumers—people curious about unusual and exotic products. The extension of the marketing
period and the integration with produce from the southern hemisphere would increase consumption in the Mediterranean countries and ensure constant presence on the
market. As for its use as a forage crop, the cloning of coldhardy and salt-resistant varieties, or varieties with a higher
protein content, as well as the introduction of this species
in balanced diets, can increase consumption in arid and
semiarid regions. The role played by platyopuntias in the
economy and the environment, considering also the scenario of global climatic change and increasing desertification, should become increasingly important (Barbera
1995).
Acknowledgment
We thank Javier Caballero and Laura Cortés, who facilitated consulting with the Banco de Información Etnobotánica de Plantas Mexicanas (BADEPLAM) of the
Jardín Botánico, Instituto de Biología, UNAM.
Literature Cited
Acuña, R., ed. 1985. Relaciones Geográficas del Siglo XVI:
Tlaxcala, Vol. II. Universidad Nacional Autónoma de
México, Mexico City.
Alcorn, J. 1984. Huastec Mayan Ethnobotany. University of
Texas Press, Austin.
Alfonso Spagna, F. 1884. La Coltivazione Forzata del Fico
d’India a Palermo. Stabilimento Tipografico Virzù,
Palermo.
Alvarez López, E. 1946. Las plantas de America en la
botanica Europea del siglo XVI. Revista de las Indias 5:
218–288.
Arias, S., S. Gama, and U. Guzmán. 1997. Flora del Valle
de Tehuacán-Cuicatlán. Fascículo 14 Cactaceae. Instituto
de Biología, Universidad Nacional Autónoma de
México, Mexico City.
Baldini, E. 1990. Fruits and fruit trees in Aldrovandi’s
“Iconographia plantarum.” Advances in Horticultural
Science 1: 61–73.
160
Casas and Barbera
Baranyovits, F. L. C. 1978. Cochineal carmine: An ancient
dye with a modern role. Endeavour, New Series, 2: 85–
92.
Barbera, G. 1994. Il Ruolo del Ficodindia nell’Agricoltura
delle Regioni Aride e Semi-aride. Atti dell’Accademia di
Scienze, Lettere ed Arti, Palermo.
Barbera, G. 1995. History, economic and agro-ecological
importance. In Agro-ecology, Cultivation and Uses of
Cactis Pear (G. Barbera, P. Inglese, and E. PimientaBarrios, eds.). FAO Plant Production and Protection
Paper 132. FAO, Rome. Pp. 1–19.
Barbera, G., F. Carimi, and P. Inglese. 1992. Past and present role of the Indian-fig prickly pear (Opuntia ficus-indica [L.] Miller, Cactaceae) in the agriculture of Sicily.
Economic Botany 46: 10–20.
Barbera G., and P. Inglese. 1993. La Coltura del Ficodindia.
Edagricole, Bologna.
Barrera, A., A. V. Barrera, and R. M. López Franco. 1976.
Nomenclatura Etnobotánica Maya. Una Interpretación
Taxonómica. Instituto Nacional de Antropología e
Historia, Mexico City.
Benz, B. F., F. Santana M., J. Cevallos E., E. Muñoz M., J.
Rosales A., and M. Rosales A. 1997. The structure and
productivity of relict stands of pitaya (Stenocereus
queretaroensis; Cactaceae), Jalisco, Mexico. Economic
Botany 51: 134–143.
Berlin, B., D. E. Breedlove, and P. H. Raven. 1973.
Principles of Tzeltal Plant Classification. Academic
Press, New York.
Biuso Varvaro, S. 1895. Il Ficodindia. Marsala Editore,
Palermo.
Bravo-Hollis, H. 1978. Las Cactáceas de México, Vol. I.
Universidad Nacional Autónoma de México, Mexico
City.
Bravo-Hollis, H., and H. Sánchez-Mejorada. 1991. Las
Cactáceas de México. Vols. II and III. Universidad
Nacional Autónoma de México, Mexico City.
Bye, R. A. 1993. The role of humans in the diversification
of plants in Mexico. In Biological Diveristy of Mexico
(T. P. Ramamoorty, R. Bye, A. Lot, and J. Fa, eds.).
Oxford University Press, New York. Pp. 707–731.
Caballero, J. 1992. The Maya homegardens of the Yucatán
peninsula: Past, present and future. Ethnoecologica 1:
35–54.
Callen, E. O. 1967. Analysis of the Tehuacán coprolites. In
Prehistory of the Tehuacan Valley. Vol. 1. Environment
and Subsistence ( D. S. Byers, ed.). University of Texas
Press, Austin. Pp. 261–289.
Candolle, A. de (1883). L’origine delle Piante Coltivate.
Fratelli Dumolard Editore, Milan.
Darwin, C. 1868. The Variation of Plants and Animals
under Domestication. John Murray, London.
Casas, A., M. C. Vázquez, J. L. Viveros, and J. Caballero.
1996. Plant management among the Nahua and the
Mixtec from the Balsas River Basin: An ethnobotanical approach to the study of plant domestication.
Human Ecology 24: 455–478.
De la Cruz, M., and J. Badiano. 1964. Libellus de
Medicinalibus Indorum Herbis. Codex Barberini.
Instituto Mexicano del Seguro Social, Mexico City.
Casas, A., B. Pickersgill, J. Caballero, and A. ValienteBanuet. 1997a. Ethnobotany and the process of domestication of the xoconochtli Stenocereus stellatus
(Cactaceae) in the Tehuacán Valley and La Mixteca
Baja, Mexico. Economic Botany 51: 279–292.
Casas, A., J. Caballero, C. Mapes, and S. Zárate. 1997b.
Manejo de la vegetación, domesticación de plantas y
origen de la agricultura en Mesoamérica. Boletín de la
Sociedad Botánica de México 61: 31–47.
Casas, A., J. Caballero, and A. Valiente-Banuet. 1999a.
Use, management and domestication of columnar
cacti in south-central Mexico: A historical perspective.
Journal of Ethnobiology 19: 71–95.
Casas, A., J. Caballero, A. Valiente-Banuet, J. A. Soriano,
and P. Dávila. 1999b. Morphological variation and the
process of domestication of Stenocereus stellatus
(Cactaceae) in central Mexico. American Journal of
Botany 86: 522–533.
Del Castillo, R., and S. Trujillo. 1991 Ethnobotany of
Ferocactus histrix and Echinocactus platyacanthus (Cactaceae) in the semiarid Central Mexico: Past, present
and future. Economic Botany 45: 495–502.
Díaz del Castillo, B. 1991. Historia Verdadera de la Conquista de la Nueva España (ca. 1568). (F. Marenco, ed.).
Editori Associati, Milan.
Donkin, R. A. 1977. Spanish Red. An ethnographical
study of cochineal and the opuntia cactus. Transactions
of the American Philosophical Society 67: 1–84.
Dressler, R. 1953. The pre-Columbian Cultivated Plants of
Mexico. Botanical Museum Leaflets 16, Harvard
University Press, Cambridge, Massachusetts.
Estrada, E. I. J. 1989. El Códice Florentino, su Información
Etnobotánica. Colegio de Postgraduados, Chapingo,
Mexico.
Felger, R., and M. Moser. 1983. People of the Desert and Sea.
Ethnobotany of the Seri Indians. University of Arizona
Press, Tucson.
Casas, A., A. Valiente-Banuet, A. Rojas-Martínez, and P.
Dávila. 1999c. Reproductive biology and the process of
domestication of Stenocereus stellatus (Cactaceae) in
central Mexico. American Journal of Botany 86: 534–
542.
Flannery, K. V., ed. 1986. Guilá Naquitz. Academic Press,
New York.
Casas, A., A. Valiente-Banuet, J. L. Viveros, J. Caballero,
L. Cortés, P. Dávila, R. Lira, and I. Rodríguez. 2001.
Plant resources of the Tehuacán-Cuicatlán Valley,
Mexico. Economic Botany 55: 129–166.
Harlan, J. R. 1992 Origins and process of domestication. In
Grass Evolution and Domestication (G. P. Chapman,
ed.). Cambridge University Press, Cambridge,
England. Pp. 159–175.
Cei, G. 1991. Viaggio e relazione delle Indie, 1539–1553. In
Nuovo Mondo, gli Italiani, 1492–1565 (P. Collo and P. L.
Crovetto, eds.). Giulio Einaudi Editore, Torino, Italy.
Hernández, F. 1959. Historia Natural de Nueva España. 7
volumes. Universidad Nacional Autónoma de México,
Mexico City.
Colunga, P. 1984. Variación morfológica, manejo agrícola
y grados de domesticación de Opuntia spp. en El Bajío
Guanajuatense. Master’s Thesis, Colegio de Postgraduados, Chapingo, Mexico.
La Mantia, T., S. Cullotta, M. Marchetti, and S. Pasta.
2001. Materiali conoscitivi per una classificazione dei
tipi forestali e preforestali della Sicilia. L’Italia Forestale
e Montana 1: 24–47.
Colunga, P., E. Hernández-Xolocotzi, and A. Castillo.
1986. Variación morfológica, manejo agrícola tradicional y grado de domesticación de Opuntia spp. en El
Bajío Guanajuatense. Agrociencia 65: 7–44.
Le Houérou, H. N. 1996. The role of cacti (Opuntia spp.)
in erosion control, land reclamation, rehabilitation and
agricultural development in the Mediterranean basin.
Journal of Arid Environments 33: 135–159.
Coppoler, S. 1827. Saggio sul ficodindia, sua coltivazione e
modo di ottenerne i frutti tardivi. Giornale di Scienze,
Lettere ed Arti per la Sicilia 57: 3–50.
Luna, C. 1999. Etnobotánica de la Pitaya Mixteca
(Pachycereae). Ph.D. Thesis, Colegio de Postgraduados,
Chapingo, Mexico.
Harlan, J. R. 1975. Crops and Man. Foundation for modern crop science series. American Society of Agronomy,
Madison, Wisconsin.
Mesoamerican Domestication and Diffusion
161
MacNeish, R. S. 1967. A summary of the subsistence. In
The Prehistory of the Tehuacán Valley (D. S. Byers, ed.).
University of Texas Press, Austin. Pp. 290–231.
Martínez, M. 1993. Catálogo de Nombres Vulgares y
Científicos de Plantas Mexicanas. Fondo de Cultura
Económica, Mexico City.
Matos, E. 1994. Mesoamérica. In Historia Antigua de
México. Vol. I. El México Antiguo, sus Áreas Culturales,
los Orígenes y el Horizonte Preclásico (L. Manzanilla and
L. López-Luján, eds.). Instituto Nacional de Antropología e Historia/Universidad Nacional Autónoma de
México/Porrúa, Mexico City. Pp. 49–73.
Mattioli, P. A. 1558. Commentarii in libro sex Pedacii
Dioscoridis de Materia Medica. Appresso V. Valgrisi,
Venice.
Mattioli, P. A. 1568. I Discorsi ne i sei Libri della Materia
Medicinale di Pedacio Dioscoride Anarbeo. Appresso V.
Valgrisi, Venice.
Monjauze, A., and H. N. Le Houérou. 1965. Le role des
Opuntia dans l’economie agricole nord-africaine.
Bullettin de l’École Nationale Superieure d’Agronomie de
Tunis 8–9: 85–164.
Nefzaoui, A., and H. Ben Salem. 2000. Opuntiae: A strategic fodder and efficient tool to combat desertification
in the WANA region. CactusNet Newsletter 2000: 2–
24.
Oviedo y Valdés, G. F. De. 1526. Sumario de la Natural y
General Historia de las Indias. Ramon de Petras,
Toledo, Spain.
Oviedo y Valdés, G. F. De. 1535. La Historia General y
Natural de las Indias. Juan Cromberg, Madrid.
Pennington, C. 1963. The Tarahumara of Mexico: The
Environment and the Material Culture. University of
Utah Press, Salt Lake City.
Pimienta-Barrios, E., and P. S. Nobel. 1994. Pitaya
(Stenocereus spp., Cactaceae): An ancient and modern
fruit crop of Mexico. Economic Botany 48: 76–83.
Rebollar, A., J. Romero, P. Cruz, and H. Zepeda. 1997. El
Cultivo de la Pitaya (Stenocereus spp.), una Alternativa
162
Casas and Barbera
para el Trópico Seco del Estado de Michoacán. Universidad Autónoma de Chapingo, Chapingo, Mexico.
Sahagún, B. 1970. El Manuscrito 218–20 de la Colección
Palatina de la Biblioteca Medica Laurenziana, Códice
Florentino. Gobierno de la República Mexicana,
Mexico City.
Sahagún, B. 1985. Historia General de las Cosas de Nueva
España. Porrúa, Mexico City.
Sahley, C. T., M. A. Horner, and T. H. Fleming. 1993.
Flight speeds and mechanical power outputs of the
nectar-feeding bat Leptonycteris curasoae (Phyllostomidae: Glossophaginae). Journal of Mammalogy 74:
594–600.
Sánchez-Mejorada, H. 1982. Las Cactáceas de México,
Secretaría de Desarrollo Agropecuario del Gobierno
del Estado de México, Toluca.
Smith, C. E. 1967. Plant remains. In The Prehistory of the
Tehuacán Valley (S. Byers, ed.). University of Texas
Press, Austin. Pp. 220–225.
Smith, C. E. 1986. Preceramic plant remains from Guilá
Naquitz. In Guilá Naquitz (K. V. Flannery, ed.).
Academic Press, New York. Pp. 265–274.
Tangiorgi Tomasi, L., and A. Tosi. 1990. “Flora e Pomona,”
l’Orticultura nei Disegni e Nelle Incisioni dei Secoli XVIXIX. Leo S. Olschki Editore, Florence.
Toledo, V. M., and M. J. Ordóñez. 1993. The biodiversity
scenario of Mexico: A review of terrestrial habitats. In
Biological Diveristy of Mexico (T. P. Ramamoorty, R.
Bye, A. Lot, and J. Fa, eds.). Oxford University Press,
New York. Pp. 757–777.
Valdes, B., Z. Diaz Lifante, and M. Martin Cacao. 1992.
The introduction of American species through Seville
past and present. Museologica Scientifica IX: 277–292.
Vargas, E. 1994. La frontera meridional de Mesoamérica.
In Historia Antigua de México, Vol. 1, El México
Antiguo, sus Areas Culturales, los Orígenes y el Horizonte
Preclásico (L. Manzanilla and L. López-Lujan, eds.).
Universidad Nacional Autónoma de México, Mexico
City. Pp. 145–174.
CHAPTER
› 10 ‹
C AC T U S PE A R F RU I T P RO D U C T I O N
Paolo Inglese, Filadelfio Basile, and Mario Schirra
Introduction
The Cactus Pear Industry Worldwide
Supply and Demand Analysis
Selling Arrangements and Methods
The Cactus Pear Orchard
Site Selection
Cultivars
Orchard Design and Planting
Plant Training and Pruning
Fruit Thinning
Fertilizer Application
Irrigation
Fruit Characteristics
Harvesting
Productivity
Out-of-Season Crop
Quality
Postharvest Physiology
Conclusions and Future Prospects
Literature Cited
Introduction
Cactus pear (Opuntia ficus-indica [L.] Mill.) is cultivated
for fruit production in both hemispheres and on all continents except Antarctica. The absence of updated statistical data from most countries in which this species is cultivated allows only a rough estimate of the worldwide land
area used — about 100,000 hectares (ha) for specialized
plantations. This figures does not include utilization of
wild plants or those cultivated for self-consumption in
home gardens or in small horticultural systems, which are
common in Mexico (nopaleras de solar), Africa, the Middle
East, South America (huertos familiares), and southern
Europe (Mediterranean gardens).
During pre-Hispanic times, cactus pear fruits were especially popular among the indigenous populations of the
arid and semiarid areas in their native Mexican highlands,
and the fruit maintained its basic role in the local diet even
163
pears occur in naturalized stands and in commercial plantations for fruit production, both for subsistence agriculture and as a cash crop. This chapter outlines the basics of
cactus pear cultivation, including economic features and
postharvest fruit management.Fgiure10n1.eahrere:
The Cactus Pear Industry Worldwide
Figure 10.1. Pruning flowers and new cladodes of Opuntia ficusindica via scozzolatura in June 2000, leading to a second bloom, in
Santa Margherita Belice, Italy. The plantation of cultivar ‘Gialla’ is
10 years old, and the plants are spaced 4 m apart along rows that are
6 m apart.
after the Spaniards conquered the Aztec empire. Cactus
pear reached the Mediterranean Basin during the 16th century. It became naturalized and the fruit was soon popular
among the rural populations (Barbera 1995). At the beginning of the 19th century, commercial plantations were established on the island of Sicily (Barbera et al. 1992) to
meet the increasing demand for late-ripening fruits, produced through scozzolatura (Fig. 10.1). This technique consists of removing the spring flush of flowers and cladodes
to get a second bloom, causing the fruits to ripen in
October to November (Northern Hemisphere), 2 to 3
months later than the summer crop (Barbera and Inglese
1993). These fruits were highly appreciated, not only by the
rural population, but also by the middle class and the aristocracy of the Kingdom of Naples (Barbera 1995). More recent is the diffusion of the species as a fruit crop into
Argentina (Ochoa 1997), California (Curtis 1977), Chile
(Sudzuki et al. 1993), Israel (Nerd and Mizrahi 1993), and
South Africa (Wessels 1988). In all these countries, cactus
164
Inglese, Basile, and Schirra
As indicated, statistics on the world area cultivated for cactus pear as well as its production, employment, and export
figures are limited. The main producing country is Mexico,
with a production of over 345,000 tons fresh mass year-1 on
about 70,000 ha of specialized plantations, concentrated
mainly in the north-central region—Zacatecas, San Luis
Potosí, Aguascalientes, Jalisco, Guanajuato — and in the
south-central region — Hidalgo, Mexico, Tlaxcala, and
Puebla (Flores Valdez et al. 1995). Italy is the most important cactus-pear-producing country in the Mediterranean
area, with about 3,500 ha of intensive plantations yielding
about 70,000 tons (Basile and Foti 1997), and 15,000 ha
overall. Sicily accounts for nearly all of the Italian production (Barbera and Inglese 1993). The area for intensive cactus pear plantations has expanded remarkably in the last
few years, leading to 35,000 tons annually in 1975–78,
48,000 tons in 1987–90, and 63,000 tons in 1997–99.
About 90% of the areas under intensive cultivation in
Sicily are in three major production zones: the San Cono
Hills, Santa Margherita Belice district, and the southwestern foothills of the Etna volcano.
The species was introduced to South Africa during the
17th century, and it became naturalized in the Cape region.
Naturalized spiny or spineless cactus pear plants are most
abundant in the Eastern Cape, where commercial cultivation is relatively unimportant. The Western Cape is South
Africa’s major deciduous fruit growing area, but there are
few commercial cactus pear orchards, even though the potential seems good, provided some supplementary irrigation is possible in the summer. Recently, expansion of
commercial orchards has occurred in the Northern
Province and the Ciskey region, stretching from Gauteng
Province to Mpumalanga (Brutsch 1997; Brutsch and
Zimmermann 1993). The total area cultivated in South
Africa is 1,000 ha, with an annual production of about
8,000 tons. In Chile, specialized plantations are cultivated
in the Santiago metropolitan area, on about 1,100 ha,
which supply over 8,000 tons of cactus pears. Cactus pear
is cultivated to a lesser extent in Argentina (800 ha and
7,500 tons), Israel (300 ha and 6,000 tons), and the United
States (200 ha and 3,600 tons; Bunch 1996; Ochoa 1997;
Nerd and Mizrahi 1993). Specialized plantations for cactus
pear exist in Brazil, Colombia, Peru, Spain, Greece, Turkey,
Jordan, Egypt, Tunisia (e.g., 16,000 ha recently planted
near Kasserine), Algeria, and Morocco, but comprehensive
statistics are not currently available.
Orchard management requires a high degree of hand
labor, with poor potential for mechanization (Basile and
Foti 1997). The labor costs, which do not include harvesting and the successive operations, range from 130 to 340
hours ha-1, of which over 70% are due to pruning, fruit
thinning, and scozzolatura (Fig. 10.1). Flores Valdez et al.
(1995) report that fruit harvest and pest control account for
most of the annual cost of a cactus pear orchard in
Mexico, while postharvest management, including fruit
marketing, account for 28 to 40% of the total cost. Profits,
although positive, vary greatly in Italy as well as in Mexico
and South America. The large number of commercial operators and the poor concentration of supply, together with
poor distribution and marketing of the product, considerably restrain the market potential. To avoid further price
drops due to an increase in the product’s supply, proper
marketing strategies must be adopted.
Supply and Demand Analysis
Demand for cactus pear in Italy is concentrated almost entirely from August to December. The reduced quantities
consumed from March to May come from the Southern
Hemisphere for those consumers seeking out-of-season
fruits. In Italy, the demand for fruit from the summer
crop and/or that belonging to a medium-low commercial
category generally comes from low-spending-capacity
consumers of cactus pears in the production zones themselves, and in those areas of northern Italy with high concentrations of southern and Sicilian emigrants, religious
communities, and the armed forces. On the other hand,
the demand for late-ripening fruits, obtained through
scozzolatura, comes from medium-to-high-spending consumers, who associate the cactus pear with exotic fruit.
Because the price of cactus pears has not fluctuated widely, the demand elasticity with respect to price is not
known, although it is not elastic for low-quality fruits, and
the demand for higher-quality fruit is more elastic. The
cactus pear demand is relatively inelastic with respect to
the earnings of consumers in lower social echelons and
tends to be elastic for well-off consumers. In Italy, more
than 25% of the cactus pears remain in Sicily, 20% go to
foreign consumers, and more than 50% are consumed in
other Italian regions, particularly where there are residents
of Sicilian origin and emigrants from producing countries
(such as North Africa), as well as pockets of high-spending consumers. In Mexico, the most important national
markets are the large cities located in the north, as well as
the medium-to-high-spending metropolitan consumers in
Mexico City, Guadalajara, and Monterey (Flores Valdez et
al. 1995).
The main factors that influence demand are: (1) presence of glochids and spines (which require adequate
postharvest de-prickling management), (2) fruit color, (3)
seed content, (4) size of the fruit, (5) time of ripening, (6)
degree of maturity, and (7) packaging. Cactus pear demand
has definitely grown both in Europe and elsewhere.
Although no precise official statistical information is available, the trend is supported by increasing exports of the
producing countries. Differences in the commercial ripening seasons and improvements in distribution have contributed to this product being consumed in many countries, over many months of the year. Indeed, at a world
level, the complementary production of the Northern and
Southern Hemispheres could potentially allow the fruit to
be present on the market all year (Inglese 1994). Mexico exports 2,000 tons, mainly to the United States and Canada,
where most of consumers are Latinos. Italy exports to
Canada, the United States, and other European countries,
such as France and Belgium and, to a lesser extent, to
Germany, the United Kingdom, and Switzerland; consumers often come from Sicilian and other Mediterranean
communities. Israel exports less than 100 tons per year,
mainly to France, while South Africa exports an undefined
amount of fruits to England and France. Chile and
Argentina have a low export level to the United States and
Canada.
The per capita consumption of cactus pears as fresh
fruit is presently at about 2.5 kg year-1 in Sicily, and
fluctuates from a few hundred grams to 1 kg year-1 for inhabitants of the other regions of Italy. Flores Valdez et al.
(1995) estimate an annual consumption of 3.7 kg year-1 in
Mexico. Minimal demand occurs for cactus pear derivative
products (jams, mostarda, ice cream, liqueurs, queso de
tuna, arrope), which are mostly concentrated in the producing areas (Sicily and Mexico), within the producing
families themselves, and for workers involved in the cultivation of the plant in production units. Most common is
the self-production for personal consumption of these
products, which also extends to confectionery craftsmanship. The development of this kind of product should play
an important role in the cactus pear industry in the near
future.
In Italy, 85 to 90% of the fruit is harvested, with
fluctuations from year to year relating to undersized fruits,
adverse meteorological conditions, and pathogens. More
than 90% of the annual crop comes from the late fruits obtained by scozzolatura, whereas the summer crop is pro-
Cactus Pear Fruit Production
165
TA B L E 1 0 . 1
Utilization and destinations of the cactus pear production in Italy, 1996–1999
Average quantities
Type
Wholesale fruit and vegetable markets
Outside the market (street vendors, large chain distributors, etc.)
Foreign markets
Farm rejects, unsold stock, weight losses, and wastage
Personal consumption
duced only in old-fashioned orchards. The ‘Gialla’ cultivar
accounts for 85% of the total production, followed by
‘Rossa’ (10%) and ‘Bianca’ (5%). About 75% of production
is represented by “prime” and “secondary” quality fruit
by weight (120–140 g); the “extra” (> 140 g) category is
about 10 to 15%, and the rest is represented by “superextra” (> 180 g) and “third” quality (< 120 g). Postharvest
facilities and infrastructure are scarce, both in Italy and
Mexico, and no more than 10% of the harvested crop is
stored in cool chambers for 2 to 6 weeks. Despite the advantages gained from expanding the period of fruit marketing, there is a substantial lack of knowledge on postharvest technologies at a commercial level.
The main destinations for the cactus pear in Italy are
the national wholesale fruit markets, followed by the
“outside the market” and the foreign markets, rejects and
wastage, and personal consumption (Table 10.1). The
“outside the market” category is increasing, and consists
mainly of street vendors. The owners of small holdings,
who become the street vendors, sell their products at higher prices, especially in cities near the production zones.
Small commercial operators serve consumer markets not
covered by the local wholesale fruit and vegetable markets.
The “outside the market” category also includes large-scale
distribution chains, which sign contracts with commercial
enterprises that have particular forms of packaging and
guarantees of consistency. This category includes mass
caterers (army canteens, hospitals, religious communities,
university and large company canteens, schools, hotel
complexes, and tourist villages), who find it more convenient to buy direct from producers in the period (autumn)
when other fresh fruit is in short supply. Only modest
quantities of cactus pears are currently absorbed by the
confectionery, pharmaceutical, and cosmetic industries, although there is a definite growth potential.Tabe10ln1.eahrere:
166
Inglese, Basile, and Schirra
tons
%
28,500
13,000
12,000
6,000
3,500
45
21
19
9
6
63,000
100
Selling Arrangements and Methods
In Sicily, the most common means of selling cactus pears
is ex farm. This form concerns about 80% of production,
and the harvesting is paid for by the buyer. In some cases,
the product is sold ex farm by weight, with harvesting
being paid for almost exclusively by the producer. Less frequently the product is sold ex market, with the producer
paying for harvesting, processing, and transport to the
place of sale.
In the ex farm sale, the contract is verbally defined 2 to
3 months or more before harvesting and stipulates that the
buyer pays 30 to 50% of the agreed-upon sum in advance,
while the remainder is payable at the beginning of harvest.
To determine the value of the goods, the quantity of goods
to be produced that year is estimated, taking into account
orchard age, layout, and management. Having agreed
upon the production volume, the sales price is established;
the buyer organizes the harvest of the product, spreading
it out over time according to processing and storage capacities, as well as market trends. The relative production
risks (e.g., climatic events) are transferred from the producer to the buyer at the moment of stipulating the contract. The buyer usually transfers the product to his own
premises directly after harvesting, so processing and packaging can be completed as quickly as possible.
Sale ex farm by weight follows the same procedures regarding the nature and form of contract, advance payments, and forms of payment. The producer agrees with
the buyer how many harvests will be produced for the
buyer and sometimes also other aspects of the form and
times of harvesting, with the general aim of allowing the
buyer to find the best placing on the market for the product. Wide price fluctuations occur in sales by weight, so the
buyer must take into consideration the categories of prod-
uct obtainable from the orchard, the care taken by the producer, and the varieties present. The risks linked to quality/quantity aspects of production remain with the producer until the goods are delivered. An ever-growing
number of cactus pear producers prefer to sell ex farm, due
to the reduced risks and the savings in time. A few producers, however, choose to sell their produce ex market,
taking upon themselves all the operations to do with production, harvesting, processing, packaging, and, at times,
storing of the cactus pears. This form is preferred by medium-to-large producers who have the necessary work force
for the various phases of processing.
In some cases in Italy, the producer harvests the fruit
and consigns it directly to the retailer; if the retailer is a
street vendor, the product goes directly to the consumer.
Some producer-dealers directly export the product, transporting it in trucks and/or ships for European destinations,
or by air carrier toward other continents. Two main articulations of distribution are: (1) producer → wholesaler →
retailer → consumer; and (2) producer → wholesaler →
selling agent → retailer → consumer. Also, an intermediary (a mediator or buying agent) can act between the producer and the wholesaler.
For the processing of cactus pears, warehouses are
equipped with fruit de-prickling machines plus selection
and sizing belts. Selection, which entails eliminating undersized fruit and those damaged by flies, transport, or split
by frosts and/or hailstorms, is carried out by specialized
staff, who also ensure the working order of the machines.
The product is packaged in plastic, wooden, or cardboard
containers with a capacity of 3 to 5 kg. Until a few years
ago, the wooden crate was the most common, whereas recently cardboard has become more common. According to
its specific destination, the product can be packed in smaller 1 to 2 kg plastic, polystyrene, or cardboard cases.
Among the forms of sale of “packed” goods, selling
directly in the warehouse prevails over selling through a
selling agent or large distribution chains. The commercial
enterprise that buys ex farm pays for the harvesting, transport of the product to the warehouse, processing, packaging, making up the pallets, and transport to the markets as
well as for the commission charges. These costs together
are U.S. $0.35 to $0.45 per kilogram. The commercial enterprises limit their marketing actions to illustrated pamphlets on the characteristics of the product, and on how to
consume the product itself. A promotion/advertising campaign over several years has been activated by the Regional
Government, with free tasting sessions of the fruit in the
main sales outlets and in the restaurants of the larger Italian
cities (Battaglia 1997). Prices at production in Sicily (Table
TA B L E 1 0 . 2
Average wholesale prices of cactus pears in the province of Catania,
Sicily, 1983–1999
Year
Current Lira
(£ kg –1)
1983
1984
1985
1986
1987
1988
1989
1990
1991
1992
1993
1994
1995
1996
1997
1998
1999
1,150
1,280
1,420
1,900
1,600
1,800
1,475
1,353
1,723
1,878
1,822
2,701
2,792
1,787
1,758
1,740
1,900
1999 Lira Value
Index
(£ kg –1)
(1983–85 = 100)
2,479
2,495
2,549
3,214
2,587
2,773
2,132
1,843
2,206
2,280
2,123
3,029
2,972
1,830
1,770
1,721
1,900
99
100
102
128
103
111
85
74
88
91
85
121
119
73
71
69
76
References: G. Timpanaro in Basile (1996) for 1983 –1995 and the
Chamber of Commerce, Industry, Craftwork and Agriculture of
Catania for 1996 –1999. These prices refer to late-ripening fruits,
which were converted to constant currency using the coefficients
calculated by ISTAT.
10.2), which refer to the fruit still on the plant with harvesting charged to the buyer, decreased in real terms by 25
to 30% from 1983 to 1999, mostly due to the expanding
supply. This, together with a rise in production costs, has
led to a drop in profits. The high values in 1986, 1994, and
1995 reflect high-quality cactus pears, indicating consumer
preference.Tabe10l2.neahrere:
The Cactus Pear Orchard
Site Selection
Opuntias are cultivated in subtropical arid areas, with mild
winters (average air temperature > 10°C) and hot summers,
where the annual rainfall ranges from 100 to 600 mm, and
with a well-defined dry season that may last 2 to 5 months.
Where no rainfall occurs during fruit development, or
where rainfall is annually less than 300 mm, cactus pear
needs irrigation to ensure economic cropping. In most
areas where cactus pear is cultivated for fruit production,
the plant has a period of no visible meristematic growth.
This period usually coincides with the winter, with its
Cactus Pear Fruit Production
167
short photoperiod and low temperatures. Indeed, high
temperatures preceding bud differentiation reduce flower
bud formation (Nerd et al. 1991; Nobel and Castañeda
1998), but the winter chilling requirement has never been
quantified. On the other hand, cactus pear plantations for
fruit production occur in the Canary Islands and in the
valley of Catamarca in northwest Argentina, where less
than 200 chilling hours accumulate during the winter.
In its native highlands of Mexico, cactus pear is cultivated in semiarid areas, where the annual rainfall is concentrated in the summer during the fruit development period. In the Mediterranean Basin, Middle East, North and
East Africa, Argentina, California, and Chile, the dry season coincides with the long and hot summer, when vegetative and reproductive growth occur. For instance, cactus
pear in Chile is cultivated in the Santiago metropolitan
area, where the average monthly air temperature is 22°C in
summer ( January) and 10°C in winter (August). The dry
season lasts 4 to 5 months, and annual rainfall averages 350
mm (Sudzuki et al. 1993). In Italy, the species is cultivated
for fruit production in Sicily, where the climate is
Mediterranean, with mild, rainy winters and hot, dry summers. The average annual temperature is 16°C, being 25°C
in July/August and 15°C in October/November during
fruit development. Annual rainfall is between 400 and 600
mm, with a 4-month dry season ( June – September). In
Israel, the species is cultivated in the Negev Desert, where
the lowest average monthly temperature is 13°C in January
and the highest temperatures (25°C) occur from June
through October; annual rainfall ranges from 40 to 200
mm. In South Africa, the Eastern Cape and the Northern
Province receive summer rainfall, and in the Western Cape
rainfall is concentrated in the winter. The Northern
Province and the Ciskey region are the most important
areas for specialized plantations, and the earliest fruit crops
come from the subtropical areas near the Tropic of
Capricorn. These provinces have long, hot summers and
mild winters, with an extremely uneven rainfall occurring
mainly in the winter and less than 500 mm annually
(Brutsch and Zimmermann 1993; Brutsch 1997). In all
areas, the average annual temperature is above 15°C, average monthly temperature ranges from 10°C in winter to
26°C in summer, and from 14°C to 25°C during fruit development and ripening.
The species occurs over a wide range of soils. A soil
depth of 60 to 70 cm ensures the development of the shallow root system. Soils with poor drainage, a high water
table, or a superficial impermeable layer or hardpan should
not be used for planting. Clay content should not exceed
20% to avoid root rotting and reduced root and canopy
168
Inglese, Basile, and Schirra
development. Cactus pear is not salt-tolerant; 50 to 70 mM
NaCl should be considered the upper threshold for
profitable growing.
Cultivars
Many species of the Cactaceae produce edible fruits
(Chapters 9 and 11). Among the approximately 1,600
species in this family, the genus Opuntia has the most relevant role in agriculture. In the Mexican highlands, the
center of genetic diversity for all opuntias (PimientaBarrios 1990; Mondragon-Jacobo and Pimienta-Barrios
1995), fruits come from wild plants of Opuntia lindheimeri
Engel., O. streptacantha Lem., O. megacantha Salm-Dyck,
and O. joconostle Web; O. amyclaea Ten. and O. ficusindica are cultivated for fruit production on about 60,000
ha of specialized plantations (Pimienta-Barrios 1990).
Natural hybrids are common in both cultivated and wild
populations (Pimienta-Barrios and Munoz-Urias 1995). In
South America, the United States, Africa, and the Mediterranean Basin, O. ficus-indica is the only species cultivated
for fruit production. Spontaneous forms have a diploid (2n
= 2x = 22) or a tetraploid (2n = 4x = 44) chromosome number, whereas cultivated varieties have a polyploid (2n = 6x
= 66 or 2n = 8x = 88) chromosome number in Mexico and
Italy (Mazzola et al. 1988; Pimienta-Barrios and MunozUrias 1995).
Cultivars for fruit production can be distinguished by
the color of the fruit peel and the ripe flesh, which can be
red-purple, yellow-orange, white-cream, or greenish. Red,
yellow, and white fruits are present in all the cultivated
areas, but green fruits, with a greenish-white flesh, are
found only in Chile and Peru (Mondragon-Jacobo and
Pimienta-Barrios 1995). Cultivars also differ in plant shape,
vigor, fertility, cladode and fruit size, fruit ripening time,
seed count, and ability to reflower (Pimienta-Barrios 1990;
Wessels 1988; Barbera and Inglese 1993). The largest diversity in plant shape occurs in the South African germplasm.
Wessels (1988) describes bushy-type, columnar (longcladode), and round-cladode plants, which also differ in
terms of vigor, chilling requirement, and cladode fertility,
and eventually require different orchard design in terms of
plant spacing. The largest genetic diversity occurs in
Mexico and South Africa, whereas in the other countries
only a few cultivars have been described and are commercially cultivated. Nevertheless, within the same cultivar,
different clones can be identified. For instance, in Italy and
Argentina, at least 3 to 4 clones of the local ‘Gialla’ or
‘Amarilla sin espinas’ are distinguishable (Barbera and
Inglese 1993; Ochoa 1997). Mexican cultivars show a great
variability also in terms of fruit ripening time, with early
ripening cultivars harvested in May (‘Tapona de Mayo’ and
‘Pachona’) or June (‘Naranjona’) and late ripening ones are
harvested in September (‘Cristalina’) or October to
November (‘Fafayuco,’ ‘Cascaron,’ and ‘Charola’). Italian,
Argentinian, and South African varieties do not show any
marked variability in fruit ripening time.
The most appreciated fruits in the international markets have a yellow-orange flesh, such as ‘Gialla’ in Italy,
‘Amarilla huesona’ in Mexico, ‘Ofer’ in Israel, ‘Malta,’
‘Gymnocarpo,’ and ‘Direkteur’ in South Africa, and
‘Amarilla sin espinas’ in Argentina. Red-purple or pink
fruits, such as ‘Algerian’ in South Africa, ‘Rossa’ in Italy, or
‘Pelon liso’ and ‘Rojo pelon’ in Mexico, are also highly appreciated, particularly in the United States, where the
‘Andy Boy’ cultivar with pink-red flesh is grown in
California (Bunch 1996). Recent studies in Italy show that
consumers unfamiliar with this fruit are attracted by red
fruits, which they buy first (Asciuto et al. 1997; Battaglia
1997). Fruits with white or greenish flesh are prized only in
regional or local markets, and their international trade is
not large. White-flesh fruits are very sensitive to postharvest handling and to specific pests, such as Ceratitis capitata
(the Mediterranean fruit fly).
Orchard Design and Planting
Cactus pear is commonly propagated via cuttings. The use
of 1-year-old potted plants has been successful in South
Africa (Wessels 1988) and Italy (Barbera et al. 1993a), but,
although the field growth response is satisfactory (Inglese
et al. 1996), additional costs are incurred for nursery establishment, plant transportation, and planting, making it
barely feasible economically. Both single and multiple
cladode cuttings are utilized.
Single cuttings can be 1 to 2 years old, and their surface
area and dry mass have a significant influence on successful rooting and subsequent budding in the field. A surface
area of 500 cm2 or a dry mass of 70 to 100 g allow good
plant growth (Barbera et al. 1993a; Inglese et al. 1996;
Wessels et al. 1997). Other sources of variability of cladode
rooting and subsequent plant growth in the field involve
the age of the mother plant and its phytosanitary conditions, cutting planting depth, the cladode surface area left
above-ground, soil temperature, and soil water content
(Brutsch 1979; Wessels et al. 1997). Adventitious roots
originate from phloem cells near the areole, while the
cambium remains dormant. The stimulus to cell dedifferentiation and multiplication may occur within 2 days
after the cuttings are placed in contact with the soil, and
primordia emergence may occur within 14 days (Fabbri et
al. 1996). The cutting may develop 60 g of root dry mass
in the first year (Inglese et al. 1996). Cuttings are usually
planted upright, with half of the cladode placed below
ground.
A multiple cladode cutting is made of a 2-year-old
cladode bearing, on its crown-edge, one or two 1-year-old
daughter cladodes. The advantage for such multiple cladode cuttings is the rapid formation of plant structure,
which results in earlier fruiting after planting. Similar results can be obtained by planting, in a single hole, two cuttings, spaced 0.4 m apart, or three to four cuttings placed
in a triangle or square and spaced 0.3 m apart. This
method ensures fast canopy development, but requires a
large amount of planting material (Mondragon-Jacobo and
Pimienta-Barrios 1995). Cuttings have to wilt 4 to 6 weeks
in a dry, shaded environment before being planted to let
the wound dry and prevent rot at the cut surface. Bordeaux
paste, as well as 0.4 milliliter of methridathion or 1 g liter-1
of copper oxychloride, are often applied to cuttings before
planting.
Late spring is the best time for planting. Indeed, roots
and cladodes reach their highest growth rate during late
spring and early summer (Barbera et al. 1993a; Wessels
1988), and soil water content in late spring is high enough
to allow root development in areas with winter rainfall,
whereas cuttings benefit from rains that occur after planting in areas with summer rainfall (Pimienta-Barrios 1990;
Barbera and Inglese 1993; Sudzuki et al. 1993; Wessels
1988). Planting at the end of the summer slows the development of the root system and canopy, due to low winter
temperatures and reduced light (Barbera et al. 1993a);
moreover, weeds compete more efficiently, and winter rains
can promote root rot. If cuttings are planted during the hot
and dry season without irrigation, root growth and budding are scarce, and cladodes readily wilt and eventually die
because of high temperatures and low humidity. Even with
summer rainfall, the time for rooting and cladode growth
is often too short.
Good orchard design, in terms of plant layout and
spacing, includes (1) hedgerow systems, with plants placed
closely along a row, and (2) a square or rectangle layout,
with plants trained to be globe-shaped and well separated
from each other. Plantations established in Italy during the
19th century were laid out in hedgerows, with plants placed
every 0.5 m along rows spaced 6 to 8 m apart (Barbera et
al. 1992a). Orchards are established with a hedgerow layout
in Israel and California (Bunch 1996), with cuttings placed
at 1.5 to 4 m intervals along rows spaced 4 to 6 m apart
(830–1,666 plants ha-1). Pimienta-Barrios (1990) suggests
a hedgerow layout for farms smaller than 5 ha, with close
spacing (2–3 m) along rows spaced 5 m apart (1,110–1,666
Cactus Pear Fruit Production
169
Figure 10.2. View of the plantation considered in Figure 10.1.
plants ha-1). Close spacing along a row increases the number of fertile cladodes per unit area in the early stages of orchard life. Close spacing results in continuous and dense
canopies, which require a high pruning frequency and intensity to avoid within-plant shading and reduction of fruit
quality. Canopies that are too dense reduce cladode fertility, facilitate cochineal infestations, and reduce the
efficiency of pest control operations.
If the plants are spaced in a square or rectangular layout, they are usually trained to a bushy-type globe shape.
In Italy, plant spacing ranges from 4 × 6 m (416 plants ha-1;
Fig. 10.2) to 5 × 7 m (290 plants ha-1). In Mexico,
Pimienta-Barrios (1990) recommends, for farms with more
than 20 ha, distances of 4 m along rows spaced 5 m apart
(500 plants ha-1). In South Africa, plants are spaced according to the cultivar growth habit. Bushy-type plants develop a continuous hedgerow because of their open growth
form. The general recommendation for bushy-type cultivars is 2 to 3 m along rows spaced 4 to 5 m apart (666–
1,250 plants ha-1). The upright types can be spaced 3 to 4
m apart along rows spaced 4 to 6 m apart (415–830 plants
ha-1; Wessels 1988). Because cladodes are generally planted
170
Inglese, Basile, and Schirra
with their planar surfaces parallel to the row direction, the
rows should be oriented north-south to maximize light interception (Nobel 1988).Fgiure102.neahrere:
Plant Training and Pruning
Cactus pear plants can be trained to a globe shape of various sizes (height and width) or canopy densities based on
cultivar growth habit, plant spacing, and environmental
conditions. In many cases, plants are not trained and are
pruned only occasionally. The globe-shaped plants have 3
to 4 main stems and a high number of fertile cladodes,
mostly distributed around the outer portion of the canopy.
Pruning can regulate resource allocation among the various
canopy sinks and can maximize light availability within the
canopy to support cladode growth, flower bud formation,
and fruit growth. Moreover, pruning facilitates pest control, fruit thinning, and fruit harvest. Garcia de Cortázar
and Nobel (1992) defined the stem area index (SAI) that
maximises plant productivity in terms of biomass. High
planting densities lead to an extremely high accumulation
of dry matter into vegetative growth, but it reduces allocation to the fruit (Garcia de Cortázar and Nobel 1992).
This is the case for orchards for nopalito production
(Chapter 13), which involve vegetative instead of reproductive growth. However, optimal SAIs for fruit production are unknown, as are strategies for annual and longterm pruning.
Even though pruning represents one of the major costs
of orchard management, information on it is scarce (Basile
and Foti 1997). Most net CO2 uptake is by current-season
and 1-year-old cladodes, with older cladodes serving as a
pool of stored carbohydrates and nitrogen that can be used
to support fruit and current-season cladode growth (Luo
and Nobel 1993; Inglese et al. 1994b; Nerd and Nobel
1995). Flower buds differentiate on terminal, well-exposed
1-year-old cladodes, whose dry weight exceeds a minimum
value for its surface area by at least 33 g (Garcia de
Cortázar and Nobel 1992).
Moreover, cladode shading affects fruit growth in
terms of size and ripening time (La Mantia et al. 1997). To
avoid alternate year bearing, every year the plant must produce the same number of new cladodes, which will bear
fruit 1 year later; new cladodes develop on 2-year-old and
even older cladodes (Inglese et al. 1998a). Thus, to get an
accurate seasonal balance between vegetative and reproductive growth, the plant needs a constant number of 1year-old cladodes (for fruit production) and 2-year-old
cladodes (for new cladode production). As a rule of
thumb, to maximize their development, no more than two
daughter cladodes should be retained on a parent cladode.
Pruning also involves the removal of the current season’s
cladodes developing on fertile cladodes. Two-year-old
cladodes, which have already produced fruit, should be
removed if there is no vegetative activity. The number of
fruiting cladodes left on a plant every year depends on
plant spacing, and ranges from 100 to 120 for 350 to 400
plants ha-1 to 20 to 30 for 1,000 to 1,200 plants ha-1. The
closer the plant spacing, the higher the pruning intensity
and frequency needed.
Pruning should be carried out when temperatures are
high enough to make the cut dry out quickly, which prevents rot and scabies. Cladodes cut at their basal edge can
be chopped and left between the rows. In South Africa,
Wessels (1988) suggests pruning from May to July after the
fruit harvest, when the plant is no longer actively growing.
New cladodes will develop the following spring, a strategy
feasible in regions with dry winters. In Mexico, PimientaBarrios (1990) suggests pruning from November to March,
during the dry and cold season. Barbera and Inglese (1993)
suggest that May to June is the best time for pruning. Plants
should be topped at 2.0 to 2.5 m in height, which avoids the
use of ladders for fruit thinning and fruit harvest.
Most plants have lower fruiting potential and cladode
renewal 25 to 30 years after planting. At this stage renewal
pruning can be an alternative to orchard replanting.
Rejuvenation can be achieved by pruning the plants back
to 4- to 5-year-old cladodes (Mulas and D’hallewin 1990).
Heavier pruning back to the lignified cladodes can also be
practiced to resume growth of weak plants. The plant generally resumes fruiting 2 to 3 years after such pruning, depending on the pruning intensity.
Fruit Thinning
The number of flower buds per fertile cladode varies according to cultivar (Barbera et al. 1991; Wessels 1992), season (Barbera et al. 1991; Nerd et al. 1993; Inglese et al.
1998a), cladode age (Inglese et al. 1994a, 1998a), and dryweight accumulation (García de Cortázar and Nobel
1992). Cladodes bearing 3 to 7 flowers are the most common and account for 50 to 60% of plant fertility (Inglese
1994), but well-exposed cladodes might bear, along their
crown-edge, 25 to 30 flower buds, most of them setting
fruits. Net photosynthesis of developing fruits is limited,
and its contribution to fruit carbon demand is greatest during the early stages of fruit development (8–10%; Inglese
et al. 1994b). Fruits obtain most of their assimilates from
their mother cladode, but the sink demand to support the
growth of fruit and the current season’s cladodes involves
a substantial flow of stored carbohydrates from basal
cladodes (Luo and Nobel 1993; Inglese et al. 1994). The
carbon demand is greatest when more than six fruits develop on 1-year-old fruiting cladodes, and particularly during the last 3 to 4 weeks of fruit growth, when the flesh rapidly develops and accumulates sugars (Barbera et al.
1992b). At this stage, the import of photoassimilate is 30%
and 70% of the fruit dry weight gain for cladodes with 5
and 15 fruits, respectively (Inglese et al. 1994b).
The fruit also competes with the current-season’s developing cladodes, which show a higher growth rate than
the fruit for most of the fruit development period. The
fruit becomes a major sink during the final swell of the
flesh, and this coincides with a consistent reduction in
the growth rate of the current-season’s cladodes (Inglese et
al. 1999). Indeed, the fruit growth rate and harvest size decrease with fruit number per cladode, when more than six
fruits are left on a cladode (Brutsch 1992; Inglese et al.
1995b). For South African cultivars, Wessels (1988) recommends retaining no more than 9 to 12 fruits per cladode to
increase fruit harvest size. Cladodes with more than 10
fruits show irregular and delayed ripening. In areas with no
rains during fruit development, thinning must be accompanied with irrigation to get a significant increase in fruit
Cactus Pear Fruit Production
171
size and percentage flesh (La Mantia et al. 1998). The effect
of fruit thinning depends also on the number of fruits per
cladode prior to thinning; the longer the time that the
fruits are retained on a cladode, the greater the effect of the
fruit number on final fruit size (La Mantia et al. 1998).
Thinning can be performed from budbreak to the early
stages of fruit development, but the most appropriate time
is 10 to 20 days after bloom, when differences in fruit size
are clear enough to allow selective thinning and flesh development is still negligible (Barbera et al. 1992b).
Removing fruits 20 to 30 days after set reduces the
effectiveness of thinning (Inglese et al. 1995b). Cladode size
(surface area and thickness) and within-canopy position
should also be considered to determine optimal thinning
ratios.
Fertilizer Application
Unlike many other fruit crops, relatively little information
concerning cactus pear nutrient demand and economy is
available (Nobel 1988). Early investigations (Monjauze and
Le Houerou 1965) demonstrate that manure application
improves Opuntia biomass productivity. Similarly, fertilization with nitrogen (N) increases biomass production
(Nobel et al. 1987), and phosphorous (P) application
significantly increases fruit production (Gathaara et al.
1989). Cladode tissue N and potassium (K) concentrations
are positively correlated, and sodium (Na) concentration
are negatively correlated with fruit yield (Karim et al.
1998). Moreover, 1-year-old cladodes that produce new organs have a higher level of nitrate at the beginning of the
season than do nonproductive cladodes (Nerd and Nobel
1995). The concentration of N in the parenchyma of fruiting cladodes decreases rapidly during fruit development,
and K concentration is higher for nonproductive than for
fruit-bearing cladodes. The magnesium (Mg) concentration is correlated with fruiting, but the calcium (Ca) content increases throughout the season, with no relation to
cladode fertility (Inglese and Pace 1999).
Extensive N application soon after harvesting the
summer crop promotes an additional budding in the autumn (Nerd et al. 1991, 1993), but it does not result in any
increase in the main crop the following summer. Injection
of KNO3 promotes flower bud induction (AguillarBecerril 1994), and increased N concentrations in fertile
cladodes reflect a rise in soluble reduced-N compounds,
which is associated with flower bud production. Neither K
nor P contents are correlated with the occurrence of the
autumn flush of flower buds (Nerd et al. 1993). Fruit mass
and soluble sugars increase with N fertilization, but high
applied N (200 kg ha-1) results in excessive vegetative
172
Inglese, Basile, and Schirra
growth (Potgieter and Mkhari 2000). Nitrate content is
highest in the parenchyma and at the basal edge of the
cladodes (Nerd and Nobel 1995).
Nutrient concentration in cladodes varies with age, position, fruit load, and season. Nitrogen concentration
varies from 0.4 to 2.2%, with the highest values occurring
in 2- and 3-year-old cladodes that serve as a reserve for the
growth of new organs (Nerd and Nobel 1995; Inglese and
Pace 1999; Potgieter and Mkhari 2000). Higher concentrations may result in excessive vegetative growth, a decrease in fertility, a delay in ripening, and a reduced fruit
color. Claassens and Wessels (1997) obtained optimum
fruit yield at a N concentration of 0.94 to 0.96%. Inglese
and Pace (1999) found the highest fruit yield per cladode
at 0.8% N. Concentrations of K and P reach 0.4 to 3.5%
and 0.06% to 0.2%, respectively. Gathaara et al. (1989)
found P and N fertilization to be beneficial to the yield of
young plants of Opuntia englemannii, at least for the first
year of growth.
Irrigation
Because of its high drought resistance and high water-use
efficiency, cactus pear is usually cultivated without irrigation. However, in areas with no summer rains and where
annual rainfall is less than 300 mm, the plants require supplementary irrigation to get adequate yields and good fruit
quality (Barbera 1984; Mulas and D’hallewin 1997). Even
in areas where summer rainfall of 300 to 600 mm is
sufficient to ensure high yields and regular fruit development, dry winter conditions may result in late and poor
flower bud induction, which in turn leads to late and low
yields (Bowers 1996; Nerd et al. 1989; Van Der Merwe et
al. 1997). Under these conditions, drip irrigation with
daily, low amounts (1–2 mm day-1) ensures high yields and
good fruit growth. Moreover, light irrigation in early
summer or during fruit swelling is desirable, particularly in
light soils, to avoid wide variations in soil moisture, which
promote fruit cracking (Wessels 1988). The counteracting
effect of water deficit on fruit size increases with the number of fruits per cladode; on the other hand, irrigation
alone cannot make up for reduced size when there are a
high (> 10) number of fruits per cladode (La Mantia et al.
1998).
Where the species is intensively grown for fruit production, irrigation is common in areas with dry summers,
particularly in Israel, Italy, and Chile. Both cladode fertility and fruit growth benefit. Barbera (1984) reports that
two to three irrigations, with an annual amount of 60 to
100 mm, applied during the earliest stages of fruit development (within 40 days after bloom), increase yield, fruit
size, and flesh percentage. Irrigation may also increase seed
weight and peel thickness (Mulas and D’hallewin, 1997);
however, there is no clear influence on sugar content, fruit
firmness, or flavor. In areas where vegetative growth occurs
during the dry period, irrigation enhances plant development in terms of both cladode number and size (Mulas
and D’hallewin 1997).
Traditional irrigation methods, such as basin irrigation,
may result in extensive leaching and are not adequate because of the shallow root system of the plants. If irrigation
is done only two to three times during the dry season, the
use of furrows may be easy and inexpensive. Localized
micro-sprinklers, which cover a relatively large soil surface
area with small volumes, meet the characteristics of the
shallow root system of cactus pear. Drip irrigation can be
also utilized, particularly when irrigation is applied during
most of the season, as in Israel. Seasonal volume ranges
from 60 to 80 mm in Italy to 250 to 300 mm in South
Africa to 500 mm supplied in Israel (Barbera 1984; Nerd et
al. 1989; Van Der Merwe et al. 1997; La Mantia et al. 1998).
NaCl in irrigation water should not exceed 25 mol m-3. Na
accumulates mostly in the roots, whereas, when using salty
water, Cl content increases both in the roots and in the
cladodes (Nerd et al. 1991).
Fruit Characteristics
Harvesting
The harvesting season for cactus pears lasts for a relatively
long time. In the Northern Hemisphere, the main summer
crop lasts from late June to mid September, depending on
cultivar and environmental conditions. The earliest crop
comes from North Africa and a late crop comes, in Italy,
from October to November (Fig. 10.3), as a result of the removal of the main spring flush (Barbera et al. 1992a; Figure
10.1). A winter crop comes in Israel, following extensive
fertilization and irrigation applied soon after harvesting the
summer crop in July to August (Nerd et al. 1993). Extremely mild winter temperatures allow a prolonged harvest season for cactus pears in Salinas, California, where
fruits are picked from September to March (Inglese 1995).
In the Southern Hemisphere, the summer crop is harvested from December to February, with a second natural crop
occurring from July through September in Chile (Sudzuki
et al. 1993) and an artificially induced second crop that
ripens in March to April in South Africa (Brutsch and
Scott 1991). In the native areas of Mexico, the harvest season varies with environment and cultivar, and goes from
May through October (Pimienta-Barrios 1990; PimientaBarrios and Munoz-Urias 1995).Fgiure103n.eahrere:
The length of the fruit development period and the
ripening time are cultivar-dependent but show large within-plant variability (Inglese et al. 1995a). Fruit ripening is
asynchronous, even at a cladode level, and two or three
pickings are required to harvest the entire crop. The harvest period of most cultivars may last for 2 to 6 weeks, depending on season and environment (Inglese 1994). The
time of flower bud burst, cladode exposure to light (La
Mantia et al. 1997), and fruit load per cladode (Inglese et
al. 1995a) are the main sources of variability of the fruit
ripening time at the plant level (Inglese et al. 1995a, 1999;
La Mantia et al. 1997). Fruit development occurs over a
wide range of climatic conditions throughout the world,
including winter and summer seasons, and the time required to reach commercial harvest maturity varies from
70 to 150 days (Brutsch 1979; Pimienta-Barrios 1990;
Nerd et al. 1991; Barbera and Inglese 1993; Inglese et al.
1999). On the other hand, the accumulated thermal time
from bloom to commercial harvest is rather constant (40
× 103 degree hours), and different accumulation patterns of
thermal time apparently account for the variability in fruit
ripening time that occur by year and environment for the
same genotype (Inglese et al. 1999).
Ripening is also sensitive to temperature. For example,
high temperatures (> 25°C) result in a rapid onset of ripening that may affect fruit size and reduce postharvest durability, whereas low temperatures (< 15°C) delay fruit ripening time and result in a prolonged fruit harvest period at
plant and orchard levels. When daily temperatures fall
below 12°C, ripening slows and fruits may overwinter and
ripen the next spring (Barbera and Inglese 1993). Temperatures above 35°C, and associated low air humidity, may
cause sunburn damage on fruits (Brutsch 1992). Differences in temperatures during fruit development involve
changes in fruit characteristics, such as size, shape, peel
thickness and color, percentage flesh, sugar content, and
seed count (Nerd et al. 1991; Barbera and Inglese 1993;
Inglese et al. 1999). Fruit should be harvested when peel
color changes, a time when the umbilical crown is still
slightly green. At this stage, fruits can withstand a substantial storage and marketing period. At harvest, the concentration of reducing sugars is 90% of that for fully ripe
fruits and should not be less than 13% by fresh weight;
pulp firmness, as measured with an 8-mm cylinder, should
not be less than 8 kg cm-2 (Pimienta-Barrios 1990; Barbera
et al. 1992b). Fully ripe fruits are too soft to be stored and
are difficult to handle.
Fruits should be harvested early in the morning, when
their internal temperature is not higher than 25°C, and
when the glochids are still wet and adhere to the peel. To
Cactus Pear Fruit Production
173
A
B
Figure 10.3. Fruit at harvest time at the end of October 2000 that resulted from scozzolatura (Fig. 10.1.): (A) fruits on cladodes and (B) fruits
ready for transport from the field.
reduce postharvest decay caused by wound-infecting
pathogens, fruits should be removed with a knife so as to
leave a small piece of the mother cladode at the edge of the
cut (Barbera and Inglese 1993). Subsequent exposure of
fruits to room temperature (curing) is recommended to
promote wound healing, the drying of the piece of cladode, and its ready detachment during handling and packaging (Chávez-Franco and Saucedo-Veloz 1985). Soon
after harvesting, fruits are cleaned and brushed under a
water spray or under suction to remove the glochids. Fruits
are usually sorted according to size, color, shape, and overall appearance. Brushing involves rudimentary equipment, but it can be mechanized to increase efficiency and
reduce fruit damage. Postharvest losses can be high, depending on cultivar, stage of maturity, environmental
conditions, and harvesting method (Castillo-Castillo and
Pimienta-Barrios 1990; Cantwell 1995; Schirra et al. 1999a).
Productivity
Fruit productivity of Opuntia ficus-indica is extremely variable from country to country. Yields of 20 to 30 tons ha-1
are reported in Israel and Italy (Barbera and Inglese 1993;
Nerd and Mizrahi 1993) and 10 to 30 tons ha-1 in South
Africa (Wessels 1988; Brutsch and Zimmerman 1993).
Much lower yields occur in Chile, 6 to 9 tons ha-1
(Sudzuki et al. 1993), and Mexico, where yields range from
4 tons ha-1 in the north to 9 tons ha-1 in the central area
(Federal District) and 20 to 25 tons ha-1 in the south
174
Inglese, Basile, and Schirra
(Pimienta-Barrios 1990; Flores Valdez et al. 1995). In
Israel, Nerd and Mizrahi (1993) report fruit yields of 18 tons
ha-1 for a 4-year-old orchard with plants spaced 4 × 1.5 m
apart. In the Salinas Valley of California, Bunch (1996) reports an average of 12 tons ha-1, with peaks of 25 tons ha-1,
for planting densities of 370 to 430 plants ha-1 (plants
spaced 6 × 4 m or 6 × 4.5 m). Impressive yields have been
measured for young O. ficus-indica cv. ‘Gialla’ plantations
in Argentina (Catamarca), where climatic conditions allow
extremely fast plant development (P. Inglese, personal observation). The wide variability in yield depends on orchard design (plant spacing), cultural practices, environmental conditions (including soil type), and cultivar
fertility.
Productivity also varies at the plant level. Differences in
planting material, related to the rooting ability of cuttings
and subsequent canopy development, account for discrepancies in yield potential 4 or 5 years after planting
(Brutsch 1979; Wessels et al. 1997). Biennial bearing—with
differences of 40 to 50% in fruit yield between off and on
years — has been reported in Italy (Barbera et al. 1991),
Mexico (Pimienta-Barrios 1990), and South Africa (Brutsch
1979), and also for natural stands of Opuntia engelmannii
in the Sonoran Desert (Bowers 1996). Mismanagement of
pruning (Inglese et al. 1998a), plant age, and interactions
between developing fruits and flower buds (Barbera et al.
1991) or vegetative versus reproductive growth (Bowers
1996) may account for this behavior.
Plants begin to yield 2 to 3 years after planting, reach
their maximum potential 6 to 8 years after planting, and
bear for 25 to 30 years or even longer, depending on pruning and overall orchard management. For a mature plant,
most (80–90%) 1-year-old terminal cladodes bear fruit and
account for 90% of the annual yield. However, they show
a wide fertility range, depending on plant age, environmental conditions, and their state of growth, as indicated
by the accumulation of dry matter relative to the cladode
surface area (Garcia de Cortázar and Nobel 1992), their orientation and exposure to light, and vegetative versus fruit
competition (Inglese et al. 1999). Two-year-old cladodes are
less fertile than 1-year-old ones; they usually account for no
more than 10% of the commercial yield, but may become
an important source of fruits in the off years and in older
plants; they have a poor ability to reflower, and their fertility usually does not exceed 6 to 10 fruits per cladode
(Nerd et al. 1993; Inglese et al. 1994a; Inglese et al. 1998a).
However, 80 to 95% of the vegetative buds, which will become fruiting cladodes 1 year after formation, differentiate
on 2-year-old cladodes (Inglese et al. 1998a).
Strategies to increase productivity involve management
to raise the number of fertile cladodes per plant and increasing the planting density. To get an annual yield of 20
tons ha-1, given a cladode fertility of 6 fruits and a fruit size
of 100 to 120 g, 28,000 to 30,000 fruiting cladodes are
needed per hectare. This means 80 to 90 fertile cladodes on
bush-type plants placed 6 × 5 m apart (335 plants ha-1) or
28 to 30 fertile cladodes per plant for high density,
hedgerow-like orchards with plants spaced at 5 × 2 m
(1,000 plants ha-1). Dry matter partitioning of mature,
fruiting cactus pear plants indicates a seasonal competition
between vegetative and reproductive growth, involving
fruits, newly developing cladodes, and the secondary
growth of older cladodes. Current season’s cladodes are the
strongest sink for most of their growth period, but the fruit
becomes the strongest sink during the last stage of fruit
growth; secondary growth is the weakest sink, and old
cladodes can contribute to the carbon budget through the
remobilization of their stored carbohydrates (Luo and
Nobel 1992; Inglese et al. 1994b). Seasonal values of harvest
index, a term indicating the relative partitioning of current
season’s dry matter to the fruits, range from 35 to 46% (excluding root growth) and are similar to those reported for
deciduous fruit crops. This demonstrates, once again, the
high efficiency of cactus pear as a fruit tree.
Out-of-Season Crop
One of the most striking features of Opuntia ficus-indica is
certainly the ability of its cladodes to reflower at different
times, naturally or after inductive practices are applied
(Nerd and Mizrahi 1997). In Chile, terminal cladodes
reflower naturally in May to June, and the resulting second
crop comes in July to September, with 50 to 60% lower
yield than the major summer crop (Saenz Hernandez 1985;
Sudzuki et al. 1993). An autumn crop also occurs in the
Santa Clara Valley of California, with fruit ripening in
winter and spring (Curtis 1977; Bunch 1996). In Israel, the
off-season crop develops on the current season’s terminal
cladodes that bear fruit a few months after extensive N fertilization (80–120 kg ha-1) and irrigation applied soon after
harvesting the summer crop in July; the amount of this
crop is rather low (20–30% of the summer crop), and decreases with plant age (Nerd et al. 1993). In Italy (Barbera
and Inglese 1993; Inglese 1994) and South Africa (Brutsch
and Scott 1991), a second flowering is obtained as a result of
the complete removal of the spring flush of flowers and cladodes. In this case, a plant produces once a year, whereas in
Chile the natural reflowering allows for two crops per year.
The spring flush removal (scozzolatura, Fig. 10.1) takes
place when the main bloom occurs, between the end of
May and the last week in June in the Northern Hemisphere, and in October in the Southern Hemisphere. The
new flower buds develop on the fertile cladodes of the natural flush, and the reflowering index, defined as the ratio
of second versus first flush flowers, is highest for cladodes
with a natural fertility of 5 to 10 flowers. The reflowering
index sharply decreases with the number of flowers in the
first flush (Inglese 1994). Removal time affects the cladode
reflowering rate (Barbera et al. 1991; Brutsch and Scott
1991). Removing flowers at a pre-bloom stage results in the
highest reflowering rate, whereas removing the spring
flush after petal shedding reduces reflowering by 50 to 70%
(Barbera et al. 1991; Inglese et al. 1998b). This decrease in
reflowering, which occurs when flowers are removed at full
bloom or after petal shedding, is related to an inhibitory
effect on flower bud initiation (Barbera et al. 1993b)
caused by gibberellic acid (GA3) diffusing from the flowers
to the mother cladode (Inglese et al. 1998b). Indeed,
Barbera et al. (1993b) demonstrate that GA3 applied within 6 days after the spring flush removal completely inhibits
reflowering, indicating that the flower bud, induced the
preceding spring (Cicala et al. 1997), is still in a reversible
stage. The current season’s developing cladodes also inhibit reflowering if they are not fully removed (Inglese et al.
1994a).
Fruits induced by removing the first flush flower buds
have the shortest development period, ripening 15 to 20
days earlier than full-bloom fruits and 30 to 40 days earlier than fruits induced after removing post-bloom fruits.
Cactus Pear Fruit Production
175
The reflowering rate also depends on the environmental
conditions at removal time, especially soil water content
and air temperature. Indeed, the extent of reflowering
greatly differs from year to year and with orchard location
(Barbera et al. 1991; Brutsch and Scott 1991; Nieddu and
Spano 1992). To improve reflowering in light soils with low
water content, irrigation should be applied at the moment
of spring flush removal. Scozzolatura should not be applied
until 3 to 4 years after planting, when reflowering ability is
still poor.
Quality
Fruit quality varies with cultivar and depends on several
management, environmental, and physiological factors.
Fruit growth potential is determined by effective pollination, hence seed count (Barbera et al. 1994; PimientaBarrios 1990, Nerd et al. 1991), but factors such as light (La
Mantia et al. 1997), water availability (Barbera et al. 1998),
temperature (Inglese et al. 1999), cladode fruit load
(Wessels 1988; Brutsch 1992; Inglese et al. 1995b), and interactions between developing fruits and cladodes (Inglese
et al. 1994b, 1999) may also play a substantial role in determining fruit growth potential, final fresh weight, and
other quality attributes. Pruning, fruit thinning, and irrigation are the most powerful tools to maximize fruit size.
Average fruit fresh weight varies with cultivar, from 100 to
240 g. Mexican cultivars such as ‘Cristallina’ and ‘Burrona’
may reach 240 g in fresh weight (Pimienta-Barrios 1990),
whereas fruit weight of the Italian ‘Gialla,’ ‘Bianca,’ and
‘Rossa’ generally ranges from 100 to 160 g (Barbera and
Inglese 1993). In South Africa, fruit fresh weight of cactus
pear ranges from 100 g (‘Algerian’) to 180 g (‘Nudosa’;
Wessels 1988).
Export size fruit must exceed 120 g. The percentage of
flesh, which should not be lower than 55%, is less variable
than fruit size (Inglese 1994; Inglese et al. 1994a). It ranges
from 60 to 65% for the Italian cultivars (‘Gialla,’ ‘Bianca,’
and ‘Rossa’), but a wider range has been found in South
Africa (Wessels 1988). In Mexico, a comparison between
nine cultivars revealed a 40 to 60% range in size (PimientaBarrios et al. 1992). Low temperatures during fruit development promote an increase in peel thickness and a reduction of flesh growth, resulting in a low flesh:peel ratio
(Nerd et al. 1993; Barbera and Inglese 1993).
Sugars, mainly glucose (6–8% on a fresh weight basis)
and fructose (5–6%), accumulate rapidly when the flesh
begins to grow, and harvest values should be at least 13%
(Barbera et al. 1992). The genotype is the main source of
variability in sugar content, with some Mexican varieties
reaching 17 to 18% (Pimienta-Barrios 1990), while crop
176
Inglese, Basile, and Schirra
load or fruit position within the canopy do not affect sugar
content as much. Low temperatures during fruit development result in a significant reduction of sugar content at
harvest (Barbera et al. 1991; Nerd et al. 1993). Organic acid
content is very low (0.03–0.12%, expressed as malic acid),
and the pH ranges between 5.0 and 7.0 (Barbera et al.
1992b; Kuty 1992).
Seed number and the ratio between empty and normal
seeds are among the most important factors defining fruit
quality. Seed number per fruit ranges from 120 to 350, and
the empty versus normal seed ratio is higher in Italian
(0.44) than in Mexican (0.11) cultivars (Barbera et al. 1994).
Seed weight changes with cultivar, ranging from 2.0 to 7.0
g per fruit (Parish and Felker 1997). The nutritional value
is similar to peach fruit (150 kilojoule per 100 g fresh
weight of digestible fraction). The ascorbic acid content is
20 to 30 mg per 100 g (Inglese 1994).
Postharvest Physiology
Cactus pear is a non-climacteric fruit with low respiration
and ethylene production rates (Lakshminarayana and
Estrella 1978; Lakshminarayana et al. 1979; Cantwell 1995),
although a preharvest climacteric-like rise in respiration rate
has been observed in fruit harvested at different stages of
development (Moreno-Rivera et al. 1979). Cantwell (1995)
indicates that fruit harvested at green, intermediate, and
ripe stages produces similar levels of CO2, but sharp increases in respiration and ethylene rates are usually detected when fruits are removed from cold storage and placed in
shelf-life simulating conditions (Schirra et al. 1997a,b). This
also depends on ripening status and storage duration
(Schirra et al. 1999b). Postharvest changes of internal quality characteristics, such as pH, titratable acidity, soluble
solids, acetaldehyde concentration, and ethanol concentration in the flesh, are low, whereas ascorbic acid concentration may decrease, depending on storage conditions.
The postharvest life span of the fruit is relatively
short. Under shelf-life conditions, cactus pear fruits may
deteriorate in a few weeks as a result of rapid aging and
decay. However, in the 19th century, when adequate storage facilities did not exist, fruits wrapped with thin paper
were preserved in dark, cool rooms for 3 to 4 months
(Bazin 1979). Covering them with straw helps reduce the
water loss rate. Rodríguez-Félix et al. (1992) found that a
total water loss of about 8% affected the fruit’s overall visual appearance. Fruit brushing to remove glochids adversely affects keeping quality and increases the rate of
water loss and decay (Testoni and Eccher-Zerbini 1990).
Common postharvest pathogens of cactus pears include
fungal infections (e.g., with Alternaria spp., Botritis spp.,
Chlamydomyces spp., Fusarium spp., Penicillium spp.) and
bacterial infections (Chessa and Barbera 1984; RodriguezFelix et al. 1992; Chapter 14).
Like most tropical and subtropical fruit species, when
stored at temperatures below 8°C, cactus pears are susceptible to physiological disorders collectively known as chilling injury, which involve the appearance of dark spots on
the peel (Chessa and Barbera 1984; Cantwell 1995). Such
postharvest responses depend upon growing conditions,
cultivar, and fruit ripening stage at harvest. For example,
fruit of ‘Verde’ cultivated in Chile is resistant to chilling injury, as no visible symptoms occur after 2 months of storage at 0°C (Berger et al. 1978), whereas fruits of Opuntia
amyclaea and O. ficus-indica from Chapingo, Mexico, have
chilling injury when stored at 8 to 10°C for 15 days
(Chavéz and Saucedo 1985), and those of ‘Gialla’ grown in
Italy exhibit severe injury after 14 days at 6°C (Chessa and
Barbera 1984; Chessa and Schirra 1990).
Preharvest spraying with gibberellic acid increases fruit
resistance to decay during cold storage but promotes susceptibility to chilling injury (Schirra et al. 1999a). Scanning
electron microscopy indicates that this effect is related to
a delay in peel maturation (Schirra et al. 1999b), and similar effects occur following preharvest sprays with 20
g/liter CaCl (Schirra et al. 1997b, 1999b). Fruits harvested
at color change are less susceptible to decay but more prone
to chilling injury than are fully ripe ones (Gorini et al.
1993). Fruits that ripen under high temperatures and low
relative humidity are highly susceptible to chilling injury
but less sensitive to rot decay, whereas fruits ripening at the
onset of the rainy season under lower temperature are sensitive to rot but less susceptible to chilling (Schirra et al.
1999b). The rate of water loss increases during cold storage
and the subsequent marketing period—and more rapidly
so in summer than in autumn fruit, presumably because of
a more rapid rate of metabolism resulting from the higher summertime temperatures (Monselise and Goren 1987).
The sharp increases in weight loss when fruits are moved
from chilling to non-chilling temperatures have been related to microscopic cracks in the rind (Cohen et al. 1994),
on which decay depends during the marketing period.
Keeping the fruits in ventilated cold rooms at 6 to 8°C
and 90 to 95% relative humidity is generally recommended for a storage life of 3 to 4 weeks (Gorini et al. 1993;
Chessa and Barbera 1984; Cantwell 1995). This is the best
compromise among preventing chilling injury, controlling
decay, reducing respiration, and reducing transpiration.
Upon longer storage, losses from rot and/or chilling injury
may increase sharply, especially when the fruits are transferred from cold storage to the market. Chessa and Barbera
(1984) found an 84% decay of autumn ‘Gialla’ fruit after a
60-day storage at 8°C, dropping to 67% at 5°C. Decay was
caused by Alternaria, Fusarium, and Penicillium spp., and,
to a lesser extent, by bacterial infections. Storage under intermittent warming to 8°C for 4 days for every 10 days at
2°C reduces chilling injury and decay after 6 weeks of refrigeration followed by 1 week at 20°C compared to storage at a constant 6°C (Chessa and Schirra 1990). Storage
at cycles of 3 weeks at 2°C followed by 1 week at 8°C halves
decay compared to continuous storage at 5 or 8°C (Gorini
et al. 1993).
Storage at 5°C under a controlled atmosphere of 2%
O2 and 2% or 5% CO2 reduces fruit decay by approximately 77%, alleviates chilling injury, and decreases water
loss, thus resulting in fruits of enhanced appearance compared to those in a standard atmosphere (Testoni and
Eccher-Zerbini 1990). Packaging with heat-shrunk polyethylene film remarkably reduces fruit weight loss, alleviates chilling injury, and results in better appearance but
does not reduce decay during 6 weeks of storage at 6°C
and subsequent marketing at 20°C (Piga et al. 1996).
Wrapping with polyolefinic film retains fruit freshness and
greatly reduces fruit weight loss during 4 weeks of storage
at 9°C and subsequent marketing (Piga et al. 1997).
Postharvest dip treatments with conventional fungicides, such as benomyl, captan, and vinclozolin, are
ineffective in controlling postharvest decay of cactus pear
fruit, although thiabendazole (TBZ) effectively suppresses decay development and mitigates expression of chilling
injury (Gorini et al. 1993). Dip treatments (2 minutes) with
a heated (48–50°C) mixture containing 250 mg/liter benomyl and 1050 mg/liter betran reduces decay during 8 weeks
of storage at 0°C and 80 to 85% relative humidity (Berger
et al. 1978). The increased efficacy of heated fungicides
reflects enhanced fungicide uptake and better coverage
(Cabras et al. 1999). Fruit dipping in 1 mg/liter TBZ at
55°C for 5 minutes significantly reduces cold- and rot-induced losses in late-crop fruits during 4 weeks of storage at
6°C followed by 1 week of marketing at 20°C, without
causing heat injury or detrimental effects to fruit firmness,
flavor, taste, or peel appearance (Schirra et al. 1996). Similar results are observed following dipping in hot water,
which also helps remove glochids—known causes of numerous micro-lesions in the skin that represent possible
entry points for wound pathogens.
Hot air treatment for 24 hours at 38°C and > 95% relative humidity reduces chilling injury (Schirra et al.
1996). For summer fruits, such a hot-air treatment reduces
decay fourfold, whereas a 48- or 72-hour treatment prior
to 3 weeks at 6°C and 1 week at 20°C halves the decay
Cactus Pear Fruit Production
177
TA B L E 1 0 . 3
Recommendations for maintaining postharvest fruit quality
Maturity indices
Peel color change (breakage) from green to yellow or red, fruit firmness, flattening of floral cavity
Quality indices
Total soluble solids (minimum of 13%, maximum of 17%, depending on cultivar), pH (6.0–6.5),
tritatable acidity (0.03–0.12%), firmness (10–12 kg cm –2), percent flesh (50–60%), fruit size
(minimum of 120 g, maximum of 240 g, depending on cultivar)
Optimum storage temperature
6 to 8°C with a storage potential of 2 to 6 weeks, depending on cultivar, ripeness stage, and preharvest environmental conditions
Optimum relative humidity
90 to 95%
Fruit respiration rates
Nonclimacteric, with low respiration rate (15–20 ml CO2 kg –1 hour –1 at 20°C)
Physiological disorders
Chilling injury, when exposed at temperatures below 5°C for more than 24 hours (pitting and
dark brown spots on the peel surface)
Pathological disorders
Fruit decay may result from peel or stem-end damage occurring during harvest and storage, or
after glochid removal; main agents are Alternaria spp., Dothiorella ribis, and Penicillium spp.
Postharvest heat treatment
(hot water dips or curing)
Conditioning fruits at 37 to 38°C for 24 to 48 hours reduces chilling injury; prestorage dipping
in water at 50 to 55 °C for 3 to 5 minutes reduces decay
Adapted from Kader (1999).
(Schirra et al. 1997a). The hot air treatments lead to better external fruit appearance and a lower weight loss, especially for fruits treated for 48 or 72 hours. Ascorbic acid
levels are generally lower in all fruits following a simulated shelf life.
The role played by heat treatments in decay control has
been related to an inhibition in spore germination and
growth, a delay that may allow the fruit to build up resistance at the wound site, and a stimulation of host-defense
responses, such as acceleration of wound healing, induction of heat shock proteins, eliciting of antifungal compounds, membrane stabilization, and changes in enzyme
activity (Lurie 1998). Ultrastructural studies have shown
that the skin surface of mature fruit displays areas with wax
layers in the form of plate-like structures (Chessa et al.
1992; D’hallewin et al. 1999) and micro-lesions in the skin
caused by glochids during fruit handling (Schirra et al.
1999b). Postharvest treatments with water at 50°C for 2
minutes or heat treatment for 24 to 72 hours at 37°C and
> 95% relative humidity apparently fuse and remodel the
epicuticular wax (D’hallewin et al. 1999; Schirra et al.
1999b)—most platelets flatten and gaps, micro-lesions, and
stomata appeared partially or completely filled with melted wax (Schirra et al. 1999b). Furthermore, early germinating spores are covered and mummified by the molten
wax. Thus, beneficial effects of heat treatments on decay
control may be due to the melting and remodelling of epicuticular wax layers and the subsequent filling of possible
entry points for wound pathogens. Table 10.3 summarizes
178
Inglese, Basile, and Schirra
recommendations for maintaining fruit quality during
postharvest management.Tabe10l3n.eahrere:
Conclusions and Future Prospects
Since the 16th century, opuntias in general and Opuntia
ficus-indica in particular have been utilized in subsistence
and market-oriented horticulture of many communities in
dry areas of Africa, Asia, Europe, North America, and
South America. However, fruit consumption is still mostly limited to local or ethnic markets, with poor export
flows (Basile and Foti 1997). Indeed, the cactus pear industry only reaches an important size in Mexico, which accounts for 70% of the cultivated area worldwide, whereas
in Argentina, Chile, Italy, South and North Africa, and the
United States, the cactus pear fruit industry is limited to
areas where natural resources do not allow the cultivation
of most alternative fruit-tree crops.
Two questions arise: What limits the horticultural potential of this species? and How can cactus pear fruit consumption be encouraged? Despite the efforts made in the
last 15 to 20 years, horticultural knowledge on this species
is still scarce, and technical and scientific information available to farmers is limited. As a result of poor and nonstandardized orchard management, great discrepancies in
yield within countries persist. Yet, rational and sustainable
orchard management makes possible the improvement and
standardization of yields and fruit quality. To improve fruit
productivity and quality, knowledge of the environmental
influence on plant fertility, fruit growth, and ripening must
be increased. Moreover, the existing genetic resources
(cultivars) should be characterized in terms of ecological
adaptation, fertility, productivity, ripening time, and fruit
characteristics.
The major factor limiting the horticultural potential of
cactus pear is the poor economic value of its fruits, which,
although appreciated in rural communities, still fail to appeal to the urban consumers of the rich European and
North American markets. Marketing and promotional
campaigns as well as consumer education should be promoted at local and international levels. An efficient distribution structure should be encouraged through international cooperation. Moreover, fruit quality standards need
to be set, and fruit quality enhanced through appropriate
orchard design and management, primarily irrigation,
fruit thinning, plant pruning, and consideration of cladode
orientation.
The presence of glochids in the peel and the hard,
thick seeds in the flesh are the major constraints for an enhancement in cactus pear consumption. Glochids can be
removed effectively soon after harvest, but technologies
must be improved and consumers need to be educated.
Eventually, selection and breeding for glochid-free cultivars
should be accomplished. The reduction of seed content is
a major challenge for future research. Growth regulators
(e.g., GA3 sprays) have been utilized to induce embryo
abortion and seed failure (Gil and Espinosa 1980), with
controversial results leading to poor chances for application
on a commercial scale. The large variability of seed number and the frequency of stenospermic seeds in many cultivated varieties provide good opportunities for selection
and breeding. Seedless selection of O. ficus-indica cvs.
‘Gialla’ and ‘Ofer’ have been described (Barbera and
Inglese 1993; Weiss et al. 1993), but they cannot be grown
profitably because of the reduced growth of the edible portion. Damigella (1958) described a spiny selection of O.
amyclaea Ten. var. leucosarca, which is seedless, because
more than 90% of its seeds are stenospermic; its whiteflesh fruits are regular in size and the percentage of flesh is
50 to 55% (P. Inglese, personal observation).
Various physical and chemical approaches have been
investigated to improve the shelf quality of cactus pear
fruit. The maximum benefit is achieved when fruit is of
top quality, harvested when properly ripe and free of defects. Moreover, packaging lines, storage facilities, transport systems, and marketing channels must be kept clean
and sanitary so as not to thwart efforts made to maintain
high fruit quality. To replace or to minimize treatments
with synthetic chemicals, research efforts are currently focused on enhancement of host resistance to pathogens via
physical, chemical, or biological inductors. Besides physical methods, heat treatments (such as hot water dips and
curing) are currently recognized as possible controllers of
postharvest diseases by direct inhibition of the pathogen
and by stimulating several host-defense responses. Yet,
while conventional fungicides are the most effective and
practical means for controlling postharvest decay of cactus
pear fruit at present, the potential of fungicides when applied at 50 to 53°C may represent a viable approach in the
future, with clear economic and environmental advantages.
Finally, international cooperation and technical assistance has given excellent results in recent years. This is the
case of the efforts of international organizations, such as
the Food and Agriculture Organization of the United
Nations, which helped the establishment in 1993 of the
International Network for Technical Cooperation on
Cactus Pear (CactusNet-FAO), with scientific members
from more than 20 countries (Pimienta-Barrios et al. 1993),
and the Professional Association for Cactus Development
(PACD), which involves scientists, growers, and professionals in the promotion of cactus pear utilization and fruit
consumption worldwide.
Literature Cited
Aguilar-Becerril, G. 1994. Response of prickly pear (O.
ficus-indica Mill.) to the injection of agrochemicals,
frost injury and root pruning. Revista de Fitotecnología
Mexicana 17: 186–193.
Asciuto, A., T. Cirivello, and M. Crescimanno. 1997.
Production and market aspects of cactus pear cultivation in Italy. Acta Horticulturae 438: 159–170.
Barbera, G. 1984. Ricerche sull’irrigazione del ficodindia.
Frutticoltura 46: 49–55.
Barbera, G. 1995. History, economic and agro-ecological
importance. In Agroecology, Cultivation and Uses of
Cactus Pear (G. Barbera, P. Inglese, and E. PimientaBarrios, eds.). FAO Plant Production and Protection
Paper 132. FAO, Rome. Pp. 1–11.
Barbera, G., and P. Inglese. 1993. La Coltura del Ficodindia.
Calderini Edagricole, Bologna.
Barbera, G., F. Carimi, and P. Inglese. 1991. The
reflowering of prickly pear Opuntia ficus-indica (L.)
Miller: Influence of removal time and cladode load on
yield and fruit ripening. Advances in Horticultural
Science 2: 77–80.
Barbera, G., F. Carimi., and P. Inglese. 1992a. Past and present role of prickly-pear Opuntia ficus-indica (L.) Mill. in
the agriculture of Sicily. Economic Botany 46: 10–20.
Cactus Pear Fruit Production
179
Barbera, G., F. Carimi, P. Inglese, and M. Panno. 1992b.
Physical, morphological and chemical changes during
fruit development and ripening in three cultivars of
prickly pear Opuntia ficus-indica (L.) Miller. Journal of
Horticultural Science 67: 307–312.
Barbera, G., F. Carimi, and P. Inglese. 1993a. Influenza
dell’epoca di impianto e del tipo di talea sulla radicazione e sullo sviluppo di barbatelle di Opuntia ficusindica Mill. Frutticoltura 10: 67–71.
Brutsch, M. O., and H. G. Zimmermann. 1993. The
prickly pear (Opuntia ficus-indica Cactaceae) in South
Africa: Utilization of the naturalised weed, and of the
cultivated plants. Economic Botany 47: 154–162.
Bunch, R. 1996. Cactus pear products at D’Arrigo Bros.
Journal of Professional Association for Cactus Development 1: 100–102.
Barbera, G., F. Carimi, and P. Inglese. 1993b. Effects of
GA 3 and shading on return bloom of prickly pear
(Opuntia ficus-indica (L.) Mill.). Journal of the South
African Society for Horticultural Science 3: 9–10.
Cabras, P., M. Schirra, V. L. Garau, and A. Angioni. 1999.
Factors affecting imazalil and thiabendazole uptake
and persistence in oranges following dip treatments.
Journal of Agricultural Food Chemistry 47: 3352–3354.
Barbera, G., P. Inglese, and T. La Mantia. 1994. Influence
of seed content on some characteristics of the fruit of
cactus pear (Opuntia ficus-indica Mill.). Scientia
Horticulturae 58: 161–165.
Cantwell, M. 1995. Postharvest management of fruits and
vegetable stems. In Agroecology, Cultivation and Uses of
Cactus Pear (G. Barbera, P. Inglese, and E. PimientaBarrios, eds.). FAO Plant Production and Protection
Paper 132. FAO, Rome. Pp. 120–136.
Basile, F. 1996. Economia della Produzione e del Mercato dei
Fichidindia in Italia. Università degli Studi, Catania,
Italy.
Basile, F., and V. T. Foti. 1997. Economic features of cactus pear production in Italy. Acta Horticulturae 438:
139–150.
Battaglia, G. 1997. A study of consumers of cactus pear in
the catering industry in central and northern Italy. Acta
Horticulturae 438: 171–180.
Bazin, R. 1979. Viaggio in Sicilia. Edizioni Ristampe
Siciliane, Palermo.
Berger, H. S., X. R. Ortuzar, C. M. Auda, M. Lizana, and
A. P. Reszczynski. 1978. Conservacion de tunas (Opuntia
ficus-indica) en almacenaje refrigerado. Investigacíon
Agricóla (Chile) 4: 21–24.
Bowers, J. E. 1996. Growth rate and life span of a prickly
pear cactus Opuntia engelmannii in the northern
Sonoran Desert. Southwestern Naturalist 41: 315–318.
Brutsch, M. O. 1979. The prickly pear (Opuntia ficusindica) as a potential fruit crop for the drier regions of
Ciskey. Crop Production 8: 131–137.
Brutsch, M. O. 1992 Crop manipulation in spineless
prickly pear (Opuntia ficus-indica) in South Africa.
Actas II Congreso Internacional de la Tuna y Cochinilla.
Universidad de Chile, Santiago. Pp. 40–47.
180
indica). Journal of the South African Society for Horticultural Science 1: 73–76.
Castillo-Castillo, M., and E. Pimienta-Barrios. 1990.
Commercialización. El Nopal: su Conoscimiento y
Aprovechamiento. Universidad Autónoma Agraria
Antonio Narro, Saltillo, Mexico. Pp. 336–343.
Chávez-Franco, S., and C. Saucedo-Veloz. 1985. Conservación en refrigeración de los variedades de tunas.
Horticultura Mexicana 1: 6–13.
Chessa, I., and M. Schirra. 1990. Prickly pear cv. ‘Gialla’:
intermittent and constant refrigeration trials. Abstracts,
XXIII International Horticultural Congress, Florence.
P. 689.
Chessa, I., and G. Barbera. 1984. Indagine sulla frigoconservazione dei frutti della cv. Gialla di ficodindia.
Frutticoltura 46: 57–61.
Chessa, I., Q. A. Cossu, and S. D’Aquino. 1992. Surface
characteristics of prickly pear (Opuntia ficus-indica
Mill.) fruit during development. In Proceedings II
Congreso Internacional de Tuna y Cochinilla. Santiago,
Chile. Pp. 86–92.
Cicala, A., A. Fabbri, A. Di Grazia, A. Tamburino, and C.
Valenti. 1997. Plant shading and flower induction in
Opuntia ficus-indica (L.) Mill. Acta Horticulturae 438:
57–64.
Brutsch, M. O. 1997. Climatic data of selected cactus pear
Opuntia ficus-indica (L.) Mill. growing areas in South
Africa. Acta Horticulturae 438: 13–20.
Claassens A. S., and A. B. Wessels. 1997. The fertilizer requirements of cactus pear (Opuntia ficus-indica (L.)
Mill.) under summer rainfall conditions in South
Africa. Acta Horticulturae 438: 73–76.
Brutsch, M. O., and M. B. Scott. 1991. Extending the
fruiting season of spineless prickly pear (Opuntia ficus-
Cohen, E., B. Shapiro, Y. Shalom, and J. D. Klein. 1994.
Water loss: A non destructive indicator of enhanced
Inglese, Basile, and Schirra
cell membrane permeability of chilling-injured Citrus
fruit. Journal of the American Society for Horticultural
Science 110: 983–986.
Curtis, J. R. 1977. The development of commercial prickly pear farming in the Santa Clara Valley, California.
Cactus and Succulent Journal (U.S.) 47: 35–37.
Damigella, P. 1958. Una nuova varietà di Opuntia amyclaea
Tenore (O. amyclaea var. leucosarca). Bolettino Istituto
Botanica Università di Catania 1: 34–45.
D’hallewin, G., M. Schirra, and E. Manueddu. 1999.
Effects of heat on epicuticular wax of cactus pear fruit.
Tropical Science 39: 1–4.
Fabbri, A., A. Cicala, and A. Tamburino. 1996. Anatomy
of adventitious root formation in Opuntia ficus-indica
cladodes. Journal of Horticultural Science 71: 235–242.
Flores Valdez, C., J. M. Esquivel, and P. Ramirez Moreno.
1995. Mercato Mundial de la Tuna. Universidad
Autónoma, Chapingo, Mexico.
Garcia de Cortázar, V., and P. S. Nobel. 1992. Biomass and
fruit production for the prickly pear cactus, Opuntia
ficus-indica. Journal of the American Society for
Horticultural Science 117: 558–562.
Gathaara, G. N., P. Felker, and M. Land. 1989. Influence
of nitrogen and phosphorus on Opuntia engelmannii
tissue N and P concentrations, biomass production
and fruit yield. Journal of Arid Environments 16: 337–
346.
Gil, G., and A. R. Espinosa. 1980. Fruit development in
the prickly pear cactus (Opuntia ficus-indica Mill.) with
preanthesis application of gibberellin and auxin.
Ciencia Investigacion Agraria 7: 141–147.
Inglese, P., A. I. Israel, and P. S. Nobel. 1994b. Growth and
CO2 uptake for cladodes and fruit of the Crassulacean
acid metabolism species Opuntia ficus-indica during
fruit development. Physiologia Plantarum 91: 708–714.
Inglese, P., G. Barbera, and T. La Mantia. 1995a. Research
strategies for the improvement of cactus pear (Opuntia
ficus-indica) fruit quality and production. Journal of
Arid Environments 29: 455–468.
Inglese, P., G. Barbera, T. La Mantia, and S. Portolano.
1995b. Crop production, growth and ultimate size of
cactus pear fruits following fruit thinning. HortScience
30: 227–230.
Inglese, P., V. Branca, and L. S. Pace. 1996. Influenza della
costrizione radicale sullo sviluppo della parte aerea e
sulla distribuzione della sostanza secca e dei carboidrati
in Opuntia ficus-indica. Atti III Giornate Scientifiche
Società Orticola Italiana. Erice, Italy. Pp. 83–84.
Inglese, P., T. La Mantia., and G. Barbera. 1998a. Alternanza
di produzione e potatura estiva nel ficodindia (Opuntia
ficus-indica L. Mill.). Atti IV Giornate Scientifiche
Società Orticola Italiana. San Remo, Italy. Pp. 485–486.
Inglese, P., T. La Mantia, I. Chessa, and G. Nieddu. 1998b.
Evolution of endogenous gibberellins at different stages
of flowering in relation to return bloom of cactus pear
(Opuntia ficus-indica L. Mill.). Scientia Horticulture 73:
45–51.
Inglese, P., B. Barbera., and T. La Mantia. 1999. Seasonal
reproductive and vegetative growth patterns, and resource allocation during cactus pear Opuntia ficusindica (L.) Mill. fruit growth. HortScience 34: 69–72.
Kader, A. A. 1999. Produce facts: Cactus (prickly) pear.
Perishable Handling Quarterly Issue 100: 15–16.
Gorini, F., A. Testoni, R. Cazzola, F. Lovati, M. G. Bianco,
I. Chessa, M. Schirra, M. Budroni, and G. Barbera.
1993. Aspetti tecnologici: Conservazione e qualità di
fico d’india e avocado. Informatore Agrario XLIX 1: 89–
92.
Karim, M. R., Felker, P., and Bingham, R. L. 1998.
Correlations between cactus pear (Opuntia spp.) cladode nutrient concentrations and fruit yield and quality. Annals of Arid Zones 37: 159–171.
Inglese, P. 1994. Biologia Fiorale e di Fruttificazione dell’O.
ficus-indica Mill. Atti Accademia Nazionale Scienze
Lettere ed Arti di Palermo, Palermo.
Kuty, J. O. 1992. Growth and compositional changes during the development of prickly pear fruit. Journal of
Horticultural Science 67: 861–868.
Inglese, P., and L. S. Pace. 1999. Contenuto in elementi
minerali e fertilità dei cladodi di O. ficus-indica Mill.
Atti Convegno “Fruttiferi Tropicali e Subtropicali in
Italia. Ragusa, Italy. Pp. 91–92.
Lakshminarayana, S., L. Alvarado, and F. Perez. 1979. The
development and postharvest physiology of the fruit of
prickly pear (Opuntia amyclaea Tenore). Tropical Food
1: 69–93.
Inglese, P., G. Barbera, and F. Carimi. 1994a. The effect of
different amounts of cladode removal on reflowering of
cactus pear (Opuntia ficus-indica (L.) Miller). Journal of
American Horticultural Science 69: 61–65.
Lakshminarayana, S., and I. B. Estrella. 1978. Postharvest
respiratory behaviour of tuna (prickly pear) fruit
(Opuntia robusta Mill.). Journal of Horitcultural Science
53: 327–330.
Cactus Pear Fruit Production
181
La Mantia, T., G. Barbera., and P. Inglese. 1997. Effect of
shading on growth and ripening of fruits of cactus pear
(Opuntia ficus-indica L. Miller). Journal of Horticultural Science 72: 299–304.
La Mantia, T., G. Barbera, G. Gugliuzza, and P. Inglese.
1998. Interazione tra irrigazione ed entità di fruttificazione e qualità dei frutti di ficodindia (O. ficusindica (L.) Mill.). Atti Giornate Scientifiche SOI. San
Remo, Italy. Pp. 241–242.
Luo, Y., and P. S. Nobel. 1993. Growth characteristics of
newly initiated cladodes of Opuntia ficus-indica as
affected by shading, drought and elevated CO2. Physiologia Plantarum 87: 467–474.
Lurie, S. 1998. Postharvest heat treatments of horticultural crops. Horticultural Reviews 22: 91–121.
Mazzola, P., S. Romano, and P. Fici. 1988. Contributo alla
conoscenza del genere Opuntia Miller. I dati cariologici e distributivi delle specie spontaneizzate coltivate in
Sicilia. Naturalista Siciliano IV 12: 159–168.
Mondragon-Jacobo, C., and E. Pimienta-Barrios. 1995.
Propagation. In Agroecology, Cultivation and Uses of
Cactus Pear (G. Barbera., P. Inglese, and E. PimientaBarrios, eds.). FAO Plant Production and Protection
Paper 132. FAO, Rome. Pp. 64–70.
Monselise, S. P., and R. Goren. 1987. Preharvest growing
conditions and postharvest behavior of subtropical and
temperature-zone fruits. HortScience 22: 1185–1189.
Monjauze, A., and H. B. Le Houerou. 1965. Le role des
Opuntia dansl’economie agricole Nord Africaine.
Extrait du Bulletin de l’École Nationale Superieure
d’Agriculture de Tunis 8–9: 85–164.
Moreno-Rivera, M. A., L. Alvarado-Sosa, and S. Lakshminarayana. 1979. Preharvest respiratory trend of the fruit
of prickly pear (Opuntia amyclaea T.). Proceedings
Journal of Florida State Horticultural Society 92: 235–
237.
182
Nerd, A., and Y. Mizrahi. 1997. Reproductive biology of
cacti. Horticultural Review 18: 321–346.
Nerd, A., and P. S. Nobel. 1995. Accumulation, partitioning, and assimilation of nitrate in Opuntia ficus-indica.
Journal of Plant Nutrition 18: 2533–2549.
Nerd, A., A. Karady, and Y. Mizrahi. 1989. Irrigation, fertilization, and polyethylene cover influence bud development in prickly pear. HortScience 24: 773–775.
Nerd, A., A. Karady, and Y. Mizrahi. 1991. Out-of-season
prickly pear: Fruit characteristics and effect of fertilization and short droughts on productivity. HortScience
26:527–529.
Nerd, A., R. Mesika, and Y. Mizrahi. 1993. Effect of N fertilizer on the autumn floral flush and cladode N in
prickly pear Opuntia ficus-indica (L.) Mill. Journal of
Horticultural Science 68: 337–342.
Nieddu, G., and D. Spano. 1992. Flowering and fruit
growth in Opuntia ficus-indica. Acta Horticulturae 296:
153–159.
Nobel, P. S. 1988. Environmental Biology of Agaves and
Cacti. Cambridge University Press, New York.
Nobel, P. S., and M. Castañeda. 1998. Seasonal, light and
temperature influences on organ initiation for unrooted cladodes of the prickly pear cactus Opuntia ficusindica. Journal of the American Society for Horticultural
Science 123: 47–51.
Nobel, P. S., C. E. Russell, P. Felker, M. Galo, and E.
Acuna. 1987. Nutrient relations and productivity of
prickly pear cacti. Agronomy Journal 79: 550–555.
Ochoa, J. 1997. Estado actual del nopal en la Republica
Argentina. VII Congreso sobre Conocimiento y Aprovechamento del Nopal, Monterrey, Mexico. Pp. 15–19.
Parish, J., and P. Felker. 1997. Fruit quality and production
of cactus pear (Opuntia spp.) fruit clones selected for
increased frost hardiness. Journal of Arid Environments
37: 123–143.
Mulas, M., and G. D’hallewin. 1990. Improvement pruning effects on vegetative and yielding behaviour in
prickly pear (Opuntia ficus-indica Mill.) “Gialla” cultivar. Acta Horticulturae 296: 139–146.
Piga, A., S. D’aquino, M. Agabbio, and M. Schirra. 1996.
Storage life and quality attributes of cactus pears cv.
‘Gialla’ as affected by packaging. Agricoltura Mediterranea 126: 423–427.
Mulas, M., and G. D’hallewin. 1997. Fruit quality of four
cactus pear (Opuntia ficus-indica Mill.) cultivars as
influenced by irrigation. Acta Horticulturae 438: 115–
122.
Piga, A., G. D’hallewin, S. D’Aquino, and M. Agabbio.
1997. Influence of film wrapping and UV irradiation
on cactus pear quality after storage. Packaging Technology and Science 10: 59–68.
Nerd, A., and Y. Mizrahi. 1993. Modern cultivation of
prickly pear in Israel: fertigation. Acta Horticulturae
349: 235–237.
Pimienta-Barrios, E. 1990. El Nopal Tunero. Grafica Nueva,
Pipila 638. Guadalajara, Mexico.
Inglese, Basile, and Schirra
Pimienta-Barrios, E., and A. Munoz-Urias. 1995. Domesti-
cation of Opuntias and cultivated varieties. In Agroecology, Cultivation and Uses of Cactus Pear (G. Barbera,
P. Inglese, E. Pimienta-Barrios, eds). FAO Plant
Production and Protection Paper 132. FAO, Rome. Pp.
58–63.
Pimienta-Barrios, E., Q. Loera, and L. O. Lopez. 1992.
Estudio anatomico comparativo en morfospecies del
subgenero opuntia. Actas III Congreso Internacional de
Tuna y Cochinilla. Santiago, Chile. Pp. 30–39.
Pimienta-Barrios, E., G. Barbera, and P. Inglese. 1993.
Cactus pear (Opuntia spp. Cactaceae) International
Network: an effort for productivity and environmental conservation for arid and semiarid lands. Cactus and
Succulent Journal (U.S.) 65: 225–229.
Potgieter, J. P., and J. J. Mkhari. 2000. Effects of N, P, K
and lime application on cactus pear (Opuntia spp.)
fruit yield and quality under rainfed conditions in
South Africa. Abstracts, IVth International Congress on
Cactus Pear and Cochineal, Hammamet, Tunisia. P. 37.
Rodriguez-Felix, A., M. I. Gonzalez-Salas, H. Soto-Valdez,
and I. Silveira-Gramont. 1992. Effects of postharvest
treatments on the quality of tuna during storage.
Proceedings of the 3rd Annual Texas Prickly Pear Council,
Kingsville, Texas. Pp. 9–21.
Saenz Hernandez, C. 1985. La tuna (Opuntia ficus-indica):
Un cultivo con perspectivas. Alimentos 3: 47–49.
Schirra, M., G. D’hallewin, P. Inglese, and T. La Mantia.
1999a. Epicuticular changes and storage potential of
cactus pear (Opuntia ficus-indica Miller (L.)) fruit following gibberellic acid preharvest sprays and postharvest heat treatment. Postharvest Biology and Technology
17: 79–88.
Schirra, M., P. Inglese, and T. La Mantia. 1999b. Quality of
cactus pear (Opuntia ficus-indica Miller (L.)) fruits in
relation to ripening time, CaCl2 preharvest sprays and
storage conditions. Scientia Horticulturae 1362: 1–12.
Schirra, M., M. Agabbio, S. D’Aquino, and T. G. McCollum. 1997a. Postharvest heat conditioning effects on
early ripening ‘Gialla’ cactus pear fruit. HortScience 36:
159–165.
Schirra, M., G. Barbera, G. D’hallewin, P. Inglese, and T.
La Mantia. 1997b. Response of cactus pear fruit to
CaCl2 preharvest treatments and prestorage heat conditioning. Journal of Horticultural Science 72: 371–377.
Schirra, M., G. Barbera, S. D’Aquino, T. La Mantia, and
R. E. McDonald. 1996. Hot dips and high temperature
conditioning to improve shelf quality of late-crop cactus pear fruit (Opuntia ficus-indica L. Mill.). Tropical
Science 36: 159–165.
Sudzuki, F., C. Munoz, and H. Berger. 1993. El Cultivo de
la Tuna (Cactus Pear). Universidad de Chile, Santiago.
Testoni, A., and P. Eccher Zerbini. 1990. Conservazione
del fico d’india in atmosfera normale e controllata.
Annali Istituto Valorizzazione Tecnologica Prodotti
Agricoli XXI, Milan. Pp. 131–137.
Van Der Merwe, L. L., A. B. Wessels, and D. I Ferreira.
1997. Supplementary irrigation for spineless cactus
pear. Acta Horticulturae 438: 77–82.
Weiss, J., A. Nerd, and Y. Mizrahi. 1993. Vegetative
parthenocarpy in the cactus pear Opuntia ficus-indica
(L.) Mill. Annals of Botany 72: 521–526.
Wessels, A. B. 1988. Spineless Prickly Pear. First Perskor
Publishers, Johannesburg.
Wessels, A. B., L. L. Van Der Merwe, and H. Du Plessis.
1997. Yield variation in clonally propagated Opuntia
ficus-indica (L.) Mill. plants when terminal cladodes
are used. Acta Horticulturae 438: 73–76.
Cactus Pear Fruit Production
183
CHAPTER
› 11 ‹
F RU I TS O F V I N E
A N D C O LU M N A R C AC T I
Avinoam Nerd, Noemi Tel-Zur, and Yosef Mizrahi
Introduction
Vine Cacti
Cytology
Pollination
Environmental Constraints
Fruit Properties
Columnar Cacti
Stenocereus queretaroensis
Other Stenocereus Species
Cereus peruvianus
Vine and Columnar Cacti as World Fruit Crops
Hylocereus
Selenicereus megalanthus
Stenocereus and Cereus
Conclusions and Future Prospects
Literature Cited
Introduction
Medium to large edible cactus fruits are produced by
members of subfamily Cactoidae, tribe Cacteae. They are
known in Latin America as pitahaya or pitaya: pitahaya
generally refers to fruits of vine (climbing) species and
pitaya to fruits of columnar (erect) ones, although considerable variation exists (Ortiz 1999). Unlike the widely cultivated cactus pear (Opuntia ficus-indica, subfamily
Opuntioideae; Chapter 10), pitayas and pitahayas have
small digestible seeds and lack the barbed small spines
(glochids) that cause difficulties in the handling of cactus
pear. Their peel is either spineless or spiny, but the spines
usually are large and easily removed upon ripening. Since
1980, an effort has been made to cultivate vine cacti in genera Hylocereus and Selenicereus and columnar cacti in genera Cereus and Stenocereus (Table 11.1, which includes
common names of ethnic origin or created by growers).
Hylocereus undatus is the most popular vine cactus and is a
worldwide crop (Table 11.1). Among the columnar cacti,
Stenocereus queretaroensis is the most cultivated, although
the growth area is restricted to central Mexico. Research on
pitahaya and pitaya crops is in its infancy. This chapter
considers the identity, environmental limits, horticultural
aspects, and commercialization of the main cultivated
species.Tabne1l.eahrere:
185
TA B L E 1 1 . 1
Vine and columnar cacti cultivated for their fruits
Species
Common names
Main cultivation countries
Fruit characteristics
Hylocereus costaricensis
(Weber) Britton & Rose
Pitahaya, pitahaya roja,
red pitahaya
Nicaragua, Guatemala
Large globose; peel dark red (rarely yellow)
with large scales; pulp deep red
H. polyrhizus
(Weber) Britton & Rose
Pitahaya, pitahaya roja,
red pitahaya,
red Eden (Israel)
Israel
Large oblong; peel dark red with large
scales; pulp violet-red
H. undatus
(Haworth) Britton & Rose
Pitahaya, pitahaya roja,
red pitahaya,
dragon fruit (Asia),
Eden (Israel)
Vietnam, Mexico,
Taiwan, Thailand,
Cambodia, Israel,
Philippines
Large oblong; peel red (rarely yellow) with
large greenish scales; pulp white
Selenicereus megalanthus
(Schun ex Vampel) Moran
(Mediocactus coccineus,
M. megalanthus)
Pitahaya,
pitaya amarilla,
yellow pitaya
Colombia, Ecuador,
Israel
Medium oblong; peel yellow with tubercles
and spines; pulp white
Cereus peruvianus (L.) Miller
(C. jamacaru, C. uruguayanus)
Pitaya, apple cactus,
Koubo (Israel)
Israel, United States
(California)
Medium-large oblong; peel smooth,
spineless, usually red; pulp white
Stenocereus griseus
(Haworth) Buxbaum
Pitaya, pitayo de Mayo
Mexico
Green or red skin; pulp reddish
S. queretaroensis
(Weber) Buxbaum
Pitaya, pitaya
de Queretaro
Mexico
Medium globose; peel with spines, green or
red; pulp white or colored, often purple
S. stellatus (Pfeiffier)
Riccobobo
Pitaya, pitaya
de Augusto
Mexico
Medium globose; peel with spines, green or
red; pulp white or colored
Vine cacti
Columnar cacti
Vine Cacti
Cytology
Chromosomes in the Cactaceae have a base number of n
= 11 (Gibson and Nobel 1986). Cultivated Hylocereus
species (H. costaricensis, H. polyrhizus, and H. undatus) and
most investigated Selenicereus species are diploids (2n = 22),
although the highly cultivated S. megalanthus (syn.
Mediocactus megalanthus or M. coccineus; Moran 1953;
Infante 1992; Weiss et al. 1995) is tetraploid (2n = 44; Beard
1937; Spencer 1955; Lichtenzveig et al. 2000). Selenicereus
megalanthus has morphological features of both Hylocereus
and other Selenicereus species (Britton and Rose 1963) and
is cross-compatible with several Hylocereus spp., suggesting
that S. megalanthus originated from an intergeneric hybridization between species of Hylocereus and Selenicereus
(Lichtenzveig et al. 2000).
No major barriers seem to limit interspecific crossing
among Hylocereus spp. Fruits obtained by such crossings
186
Nerd, Tel-Zur, and Mizrahi
are large, and the majority of the seeds germinate (Weiss et
al. 1994b). Their hybrids show a normal meiosis, with 11
bivalents at metaphase I, and produce pollen of high viability and seeds with high germination rates (Lichtenzveig
et al. 2000). Pollen viability for S. megalanthus is significantly lower than that for Hylocereus spp. and is associated
with the pairing of multivalents and consequently the occurrence of univalents at metaphase I, which leads to
pollen or ovules with chromosomal disorders (Lichtenzveig
et al. 2000). Selenicereus megalanthus crossed with Hylocereus hybrids are triploids, aneuploids, or polyploids with
different chromosome numbers (N. Tel-Zur, S. Abbo, and
Y. Mizrahi, unpublished observations). The ability to produce hybrids by interspecific and intergeneric crosses is
utilized in Israel for breeding cultivars with desired fruit
characteristics (such as improved taste) or greater environmental flexibility. Modern molecular techniques (Chapter
15) may elucidate the genetic relationship among vine cacti
underlying such features. An effective procedure has been
developed for DNA extraction from Hylocereus and
Selenicereus (Tel-Zur et al. 1999).
Pollination
Studies of breeding systems conducted in Israel (Weiss et
al. 1994a; Nerd and Mizrahi 1997; Lichtenzveig 2000)
show that Hylocereus spp. are self or partially incompatible,
and foreign pollen (pollen of other Hylocereus spp.) is
required for fruit set. Selenicereus megalanthus is selfcompatible, as fruit can set with its own pollen. However,
in certain clones larger fruit are obtained using pollen of
other S. megalanthus clones, indicating partial self-incompatibility (Lichtenzveig et al. 2000). Climate conditions
can also affect compatibility; at the end of the warm Israeli
summer, high temperatures decrease H. undatus fruit set
with self pollen. Natural pollinators of vine cacti seem to
be bats and hawkmoths (Nerd and Mizrahi 1997). In orchards of vine cacti planted in the tropics, pollination is
achieved without human intervention. In Israel, pollination is done by hand due to a lack of pollen vectors (Weiss
et al. 1994a). Pollen preservation (Metz et al. 2000) allows
for cross-pollination for times when there is no synchronization between the flowering of different Hylocereus
crops.
Environmental Constraints
Vine cacti are native to tropical regions of North and South
America, using their triangular slender stems, equipped
with adventitious roots, to spread on trees. They are successfully cultivated outdoors in cloudy tropical regions
(Central America and the Far East); e.g., H. undatus grows
successfully in southern Vietnam and Taiwan without any
shading (Fig. 11.1A). However, in Israel, where solar radiation is high (noon photosynthetic flux densities can reach
2,200 µmol m-2 s-1 in the summer), plants exposed to full
sunlight can bleach, degenerate, and produce low quality
fruit with poor color and low sugar concentrations (Raveh
et al. 1997, 1998; Mizrahi et al. 1997; Mizrahi and Nerd
1999). Hence, plants are maintained under shade nets (Fig.
11.1B). Certain species, such as H. costaricensis and H.
polyrhizus, have a higher light tolerance, probably due to
their waxy thick skin that reduces the transmittance of light
to the inner tissues of the stem. Orchards for such species
in Israel are established in net houses with shading ranging
from 20 to 60%—the higher shading being used in warmer
regions (Mizrahi and Nerd 1999). The intensity of solar radiation changes during the daytime and depends on season,
so control of light should be considered. Plants of
Hylocereus spp. exposed to full sunlight in the spring have
early blooming and early seasonal fruit production.nhF1egar.eu1i:er
Vine cacti are sensitive to chilling temperatures and the
stems bleach when night temperatures decline below
about 5°C. Severe injuries appear when air temperatures
approach zero; round yellow lesions develop along the
stems and stem segments die. Subfreezing temperatures
(–3°C) kill most of the collected germplasm in Beer-Sheva,
Israel (Mizrahi and Nerd 1999). Observations in BeerSheva show significant differences among species in cold
tolerance, as frost events totally damage Hylocereus sp.
#10487 (unidentified species cultivated in Israel) and
Selenicereus megalanthus, moderately damage H. undatus,
and slightly damage H. costaricensis and H. polyrhizus.
Plants recover easily when temperatures rise, but yields are
reduced. In areas with cold temperatures, plastic coverings
or glasshouses are recommended (Mizrahi and Nerd 1999).
In Israel water and fertilizer are commonly applied by
drip irrigation almost year-round, except in the rainy periods (Nerd and Mizrahi 1998; Nerd et al. 1999). Vegetative
growth and fruit production in the hot internal valleys
( Jordan and Arava) occur later than in the more moderate
coastal region. A well-developed canopy (1.8 m high in
rows that are 2.5 m apart) is obtained for H. undatus at the
3rd year in the coast region, whereas 5 to 6 years is required
in the valleys; annual fruit yield of the full canopy orchard
is 32 to 40 tons ha-1 at the coast versus 2 to 3 tons ha-1 in
the warmer and drier valleys. Because the rates of fruit-set
and the average fruit weight are similar for the valleys and
the coast, the small number of flowers produced in the valleys is likely the predominate factor in the low yields. The
high summer temperatures (monthly maximum/minimum air temperatures are 38/25°C, 7 to 10°C higher than
those at the coast) or the saline irrigation water in some locations (electrical conductivity of soil water = 3–4 decisiemens m-1 at Arava versus 1 dS m-1 near the coast)
probably inhibits flower induction. High summer temperatures in the valleys cause daily net CO2 uptake to be
small or negative for vine cacti (Raveh et al. 1995), so
deficiency of photosynthate can also explain the slow
growth rate and low fruiting in the valleys.
In response to high air temperatures (43–46°C) that
occur during warm summers in Israel, stems can turn
brown and become liquefied (Fig. 11.1B). Hylocereus undatus is the most sensitive of the cultivated vine cacti; in the
summer of 1998 in the Arava Valley, about 50% of its total
stem length was damaged versus less than 10% for H.
costaricensis and H. polyrhizus, and none for S. megalanthus. Similar results occur for a germplasm collection that
is located in a greenhouse in Beer-Sheva under unusual extreme summer temperatures (Mizrahi and Nerd 1999).
Shade reduces the heat damage.
Fruits of Vine and Columnar Cacti
187
A
B
Figure 11.1. (A) Hylocereus undatus on single-plant trellis system in Taiwan and (B) H. undatus (on left) and Hylocereus polyrhizus (on right) on
row trellis system under shade net (shade cloth) in Israel, where injury occurred on H. undatus at high summer temperatures of 45°C.
Fruit Properties
Fruits of vine cacti are medium-sized to large berries with
a thin, colored peel and white or colored, juicy pulp
(Table 11.1) containing numerous small, soft, digestible
seeds. The peels of Hylocereus spp. have scales that contribute to the attractive appearance of the fruits, which
also are used decoratively. For Selenicereus megalanthus, the
peel is covered with spiny tubercules, but the spines are
readily shed upon ripening. Fruits of Hylocereus crops are
usually large (up to 1,000 g), two- to threefold heavier
than those of S. megalanthus.
Studies in Israel (Weiss et al. 1994b; Nerd and Mizrahi
1998, 1999; Nerd et al. 1999) show that fruit growth for H.
costaricensis, H. polyrhizus, H. undatus, and S. megalanthus
follows a sigmoid pattern, with a low or negligible growth
rate during the last phase when ripening occurs. A change
in peel color (color-break) indicates the beginning of the
last phase. For H. polyrhizus, which has a red-violet pulp,
the accumulation of pulp pigments occurs in parallel with
the development of peel color. During the last phase, pulp
content (as a percentage of fruit fresh weight) increases
markedly (from 20–30% at the beginning to 60–80% at
the end of the phase), pulp titratable acidity declines, and
pulp contents of soluble sugars and soluble solids increase
(reaching maximal levels of 7–9% and 14–18%, respectively, of the fresh weight at full color). Degradation of
starch, which increases in the pulp prior to ripening, accounts partially for the accumulation of soluble sugars. For
H. undatus, amylase and invertase activity is correlated
with the increase in pulp soluble sugars (mainly fructose
and glucose), which reach their highest concentrations at
the center of the pulp (Wu and Chen 1997). The fruits are
classified as nonclimacteric, reflecting the low production
rates of CO2 and ethylene during ripening (Nerd and
Mizrahi 1998; Nerd et al. 1999).
Palatability tests on fruits picked at different ripening
stages (determined according to the number of days after
anthesis or the appearance of peel color) indicate that fruits
are most palatable at advanced ripening stages or at full
color. Similar to many other fleshy fruits, growers tend to
harvest the fruits of vine cacti prior to full ripeness when
the peel is still mostly green in order to prolong the marketing life of the fruit (Barbeaue 1990; Cacioppo 1990).
However, ripening of such fruits is not as good compared
with those left to ripen on the vine. For example, fruits of
S. megalanthus picked at color-break and held at 10 to
20°C attain the physical appearance of fruits ripened on
the plant and their acidity decreases, but the soluble sugars remain low and the flavor is poor (Nerd and Mizrahi
1999). Hence, the optimum stage for harvesting fruits of
vine cacti for high consumption quality under usual storage conditions should be at close to full ripeness, as evidenced by almost full or full color development.
The shelf life of fruits of Hylocereus species is about 7
to 10 days at room temperature (about 20°C; Nerd et al.
1999). Shelf life is limited by senescence symptoms, such as
a sharp decline in acidity and sugars, scale yellowing and
shriveling, and fruit softening. Fruits can be stored for 14
days at 10 to 12°C and longer under lower temperatures
(4–6°C), but upon transfer to room temperature they tend
to develop chilling injury symptoms, such as peel browning and decay. Similarly, fruits of S. megalanthus are sensitive to chilling temperatures, but the storage life (at 10–
12°C) is at least twice as long as for Hylocereus fruits (Nerd
and Mizrahi 1999).
Vine cacti produce several floral flushes during the
flowering season, and predicting the harvest time is important for orchard management and fruit marketing
(Mizrahi and Nerd 1999). Little is known about the effect
of plant and environmental factors on the duration of fruit
growth for vine cacti, but temperature appears to be a
dominant factor. In Israel Hylocereus spp. produces three or
four flushes during the warm season ( June–October); 30
to 35 days elapse from anthesis to full fruit color when daily
temperatures average 25°C, but 40 to 45 days are required
when daily temperatures average 20°C (Nerd et al. 1999).
Flowering of S. megalanthus in Israel occurs mainly in the
autumn, when air temperatures decrease and the duration
of fruit development is much longer than for Hylocereus
spp. The increased development time is due to the lower
temperatures during fruit growth and the lower inherent
growth rate for the fruit of S. megalanthus (Nerd and
Mizrahi 1998). The time from anthesis to full ripening for
S. megalanthus varies from 120 days (average daily temperatures of 25°C) to 180 days (20°C). The positive correlation
between air temperature and fruit growth enables the development of a heat-unit model to predict the period from
anthesis to maturity for S. megalanthus (Nerd and Mizrahi
1998).
Columnar Cacti
Columnar cacti are much less cultivated for fruit than are
vine cacti, and little is known about their environmental
flexibility (Mizrahi et al. 1997). Among them, Stenocereus
spp. are the most cultivated. They are planted in Mexico in
or near their natural habitats, which have a semitropical
climate with both summer (about 65%) and winter rains
for a total of 400 to 800 mm annually (Pimienta-Barrios
and Nobel 1994; Pimienta-Barrios et al. 1997). Monthly av-
Fruits of Vine and Columnar Cacti
189
erages of daily air temperatures range from 8 to 18°C at
night and from 24 to 34°C during the daytime. Temperature fluctuations from the coldest to the warmest month is
only 8°C at any particular site. Each species will be considered separately.
Stenocereus queretaroensis
Stenocereus queretaroensis is probably the commercially
most important species of the genus (Pimienta-Barrios and
Nobel 1994). It is also the most investigated (Nobel and
Pimienta-Barrios 1995; Pimienta-Barrios et al. 1997;
Pimienta-Barrios 1999). Fruits reach the local markets from
three sources: (1) wild stands, (2) wild stands enriched with
cuttings of selected clones (managed in situ), and (3) cultivated selected clones established from cuttings in home
gardens and small plantations (Fig. 11.2). Many commercially productive stands come from relict ones, namely
those associated with archeological sites dating back as long
as 2,300 years BP (Benz et al. 1997). Today’s plants are
offspring from ancestors selected in pre-Columbian times,
with better horticultural features having evidently gone
through selection by humans for many years (Benz et al.
1997).Fgiure21.neahrere:
Fruit weights of S. queretaroensis average 100 to 200 g.
The pulp is very tasty, has different colors according to the
clone (white, pink, orange, red of various hues, and purple), and contains soft edible small seeds. The spines are
soft, abscise upon ripening, and can easily be removed by
hand. The best clones are found in relict stands near archeoligical sites, and fruit productivity per plant correlates
with the canopy width, probably due to length of the
branches on which the fruits are produced. Annual production of 100 fruits per plant yields about 17 tons fresh
weight ha-1 year-1 from the best orchards (Benz et al. 1997).
The best-known clone is ‘Mamey,’ whose fruit can reach
the size of 165 g fresh weight. The peel is 18 to 24% of the
fruit fresh weight under cultivation, which is better (lower)
than for the much more common cactus pear, whose peel
is about 45% of the fruit fresh weight (Pimienta-Barrios et
al. 1997). The fruit shelf life is only a few days, partly because the fruits split (dehisce) and the pulp is exposed to
contamination by bacteria and fungi. This very short shelf
life, even for non-splitting fruit, is the main constraint on
its commercialization (Pimienta-Barrios et al. 1997). In
spite of this shortcoming, workers involved with this fruit
earn the same to three times as much as other wage earners in the region (Benz et al. 1997). Another important factor that limits the domestication of S. queretaroensis is its
relatively low growth rate. Ten years are required for an orchard to obtain large enough fruit production to reach
190
Nerd, Tel-Zur, and Mizrahi
Figure 11.2. Orchard of Stenocereus queretaroensis in southwestern
Jalisco, Mexico. Photograph courtesy of Park S. Nobel.
profitability. On the other hand, 100 years of production
are expected from this plant, reaching its peak at 40 years
of age (Pimienta-Barrios et al. 1997). This pitaya does not
respond to traditional management, such as irrigation and
fertilization, possibly due to symbiotic association with
mycorrhizae or genetic inflexibility (Pimienta-Barrios and
Nobel 1995; Pimienta-Barrios et al. 1997).
Stenocereus queretaroensis in Mexico flowers in February
and March, the beginning of the dry season. Fruits ripen
mainly in May and are harvested at the ripe stage. The soluble sugars comprise 10 to 11% of the fruit fresh weight for
various cultivars (Pimienta-Barrios et al. 1994, 1997). The
fruits are sour (pH 4.5–5.0), with the acid content ranging
between 0.15 to 0.5% of the fruit fresh weight. Fruits of S.
queretaroensis have to be sold immediately because of a
very short storage and shelf life, as they readily split open
(Pimienta-Barrios and Nobel 1994; Pimienta-Barrios et al.
1997); this, as already indicated, is the main restricting factor in the commercialization of this species.
Other Stenocereus Species
Stenocereus stellatus is the commercially second most
important columnar cactus in Mexico. Known locally as
“xoconochtli,” archeological evidence shows that it was
consumed by humans as long as 7,000 years BP (Chapter
9). Today S. stellatus has the same three major sources of
fruits as for S. queretaroensis (Casas et al. 1997, 1999a,b). It
is grown in semitropical areas with average annual temperatures of 17 to 24°C and annual rainfall of 440 to 760
mm. Fruit weight is less than that of S. queretaroensis, ranging from 20 to 80 g; the number of fruits per plant varies
from 12 to 187. The maximum density of plants in home
gardens is 780 ha-1, and the highest yield is 3.3 tons ha-1
year-1. Fruits vary in skin color—some are green (preferred
in home gardens) and most are red. Pulp color is red in
wild ones, whereas under cultivation pink, purple, yellow,
orange, and white pulp can also be found. In the wild the
taste is usually sour, whereas cultivated fruits are sweet and
sometimes insipid. Some fruits are very spiny, but others
have few spines. Upon ripening, spines can be easily removed. Locals prefer the less spiny, larger sweet fruits with
a green peel and white flesh (Casas et al. 1997, 1999a).
Plantations established from cuttings (about 1 m in length)
bear abundant fruits during the fourth year, although some
fruits may be obtained one year after planting but are followed by no fruits in the second year (Casas et al. 1997). In
the Negev Desert of Israel, even under fertigation (irrigation with water plus specific nutrients), S. stellatus produces low amounts of small fruits with no promise of
being a commercial orchard crop (Nerd et al. 1993). As for
many other columnar cacti, this species flowers nocturnally
with self-incompatibility. Also, some genotypes cannot
pollinate each other, suggesting that S genes are active
(Casas et al. 1999b).
Stenocereus griseus is of a more tropical nature than the
other cultivated Stenocereus species (Silvius 1995). Its common name, Pitaya de Mayo, means ripening in May
(Mizrahi et al. 1997). The fruit is relatively large (100–200
g) and of good quality, similar to other Stenocereus spp. In
Venezuela there are two ripening seasons, May–early June
and late August – early September, corresponding to the
rainy seasons (Silvius 1995). In Israel when regularly fertigated (Nerd et al. 1993), flowers appear in late April to May
and again in July and in October. Fruits occur in late May
(hot valleys) and June to August, but no fruits occur from
the October flowering, possibly because temperatures at
this time will not support fruit growth. It is sensitive to subfreezing temperatures and has a poor tolerance to salinity
(Nerd et al. 1993). Stenocereus griseus is harvested and marketed only in Mexico (Pimienta-Barrios and Nobel 1994).
Cereus peruvianus
Cereus peruvianus has only recently been domesticated and
its origin is obscure, although possibly it comes from Brazil
(Mizrahi and Nerd 1999). Cereus jamacaru from north-
eastern Brazil and C. uruguayanus from Argentina are
closely related species or even the same one (Taylor and
Zappi 1992; R. Kiesling, personal communication). The
species is common in gardens in tropical and subtropical
countries and has been planted commercially on a small
scale in Israel. In comparison to various Stenocereus spp. examined in Israel, C. peruvianus has significantly higher
growth and precocious yielding (Nerd et al. 1993). The
common commercial trade name given by the Israeli export company AGREXCO is ‘Koubu’ to distinguish between the fruit of this species and other pitayas (Mizrahi
and Nerd 1999).
Growth and fruiting of C. peruvianus in Israel are
significantly higher near the coast than in the internal
warm valleys or the cold Negev Highlands, where frost (air
temperatures decline to –7°C) damages the plants (Nerd
et al. 1993). The species is sensitive to salinity (electrical
conductivity of the irrigation water of 4 dS m-1), particularly when Na and Cl are the predominating ions (Nerd et
al. 1993). Israel uses C. peruvianus as a fruit crop, but
in South Africa it is considered a weed (Moran and
Zimmerman 1991). In Brazil it is used for its valuable gum
and polysaccharides (Alvarez et al. 1992, 1995), and various
vegetative micropropagation techniques have been developed (Deoliveira et al. 1995; Machado and Prioli 1996).
Cereus peruvianus is self-incompatible and requires crosspollination to obtain fruits (Weiss et al. 1994a; Silva and
Sazima 1995; Nerd and Mizrahi 1997). In spite of its nocturnal flower opening, daytime-active honey bees can act
as pollinators using the few hours the flowers are open in
the late evening or early morning (Weiss et al. 1994a; Nerd
and Mizrahi 1997; Mizrahi and Nerd 1999). However,
when large plants produce hundreds of flowers in a wave
in Israel, honey bees fail to cross pollinate the flowers
efficiently; hand pollination is then needed to obtain high
fruit set and large fruits.
Cereus peruvianus during the warm season in Israel
produces several flower flushes. Fruit development differs
significantly from that of vine cacti, exhibiting a double
sigmoid growth curve with an early and a late rapid growth
phase and a slow intermediate growth phase (Weiss et al.
1994a; Wang 1997). Color-break designates the beginning
of the last phase, and fruits are fully colored at the end of
this phase. During the last phase, pulp fresh weight increases significantly, whereas peel fresh weight remains almost constant; for fully developed fruits, the pulp comprises 70 to 80 % of the fruit fresh weight versus 15 to 20%
for young fruits. Ripening occurs during the last phase;
firmness and acidity decrease and soluble sugars and total
soluble solids increase (to 8–10% and 12–13% of pulp fresh
Fruits of Vine and Columnar Cacti
191
Figure 11.3. Fruits of H. undatus packed in Israel for export to Europe.
weight, respectively) at full color (Wang 1997). Similar to
vine cacti, the optimum harvest stage is when the fruits are
close to or at full ripeness. Fruits of C. peruvianus tend to
crack during ripening and in certain genotypes black
areas appear on the peel when fruits mature or are touched
during harvest. The tendency for both disorders is genetically inherited and can be overcome by clonal selection.
For C. peruvianus in Israel, about 40 days elapse from anthesis to full color when daily temperatures average 28°C
versus 42 to 47 days when temperatures average 24°C
(Wang 1997). Fruits of C. peruvianus are similar to those of
S. megalanthus with regard to storage and shelf life.
Vine and Columnar Cacti as World Fruit Crops
Hylocereus
The most widely cultivated vine cactus is the red pitahaya,
Hylocereus undatus (red peel, white pulp; Fig. 11.3). Its utilization as a major commercial fruit crop has its origins in
Vietnam (V. Van Vu, personal communication). The plant
was introduced to Indochina by the French around 1860,
192
Nerd, Tel-Zur, and Mizrahi
where it is now considered to be a native plant. Orchards
in Vietnam are established from selected clones, and the estimated cultivated area in southern Vietnam is 6,000 ha.
The fruits produced (“dragon fruit,” or thang loy in
Vietnamese) are common in local markets and are also exported to Asian and European countries. Hylocereus undatus is now spreading in many Asian countries, such as
Thailand, Laos, Indonesia, Cambodia, Taiwan, and recently Japan (Okinawa), using clones from Vietnam.
Horticultural research in Taiwan indicates a strong local interest in the fruit (Feng-Ru and Chung-Ruey 1997a,b).
Other vine pitahayas, such as H. costaricensis and S. megalanthus, were also introduced to Taiwan and are used for
both cultivation and breeding. In addition to the fruit, fresh
and dry flowers are also consumed in Taiwan as a vegetable.
The development of the H. undatus industry is also very intensive in Mexico (Ortiz 1999; Canto 2000), and the estimated present planted area there is about 2,000 ha, half of
it in the Yucatan peninsula where it can be found in the
wild and has been utilized from pre-Columbian times.
Most of the crop in Mexico is sold in local markets.nF3hgea.u1ire:r
Area
Yield
area (ha)
15
100
10
50
5
0
1994
1995
1996
1977
1998
1999
0
2000
yield (tons fresh weight)
150
20
year
Figure 11.4. Increase in plantation area and fruit yields of vine cacti
in Israel during the 1990s.
In Israel the cultivation of H. undatus is expanding rapidly, reaching nearly 20 ha in 2000. Export to Europe started in 1996 with 10 tons and in 1999 was about 130 tons
(Fig. 11.4). Under the dry climate of Israel, fruit quality is
high and most of the harvested crop is marketable. The
main difficulties for export are the short storage life of the
fruit and the non-stable fruit supply; flowering and harvests occur in waves (Mizrahi and Nerd 1999). Also other
countries, such as Australia, New Zealand, the United
States (Florida and California), Spain, and the Philippines,
are developing the cultivation of H. undatus.Fgiure41.neahrere:
Hylocereus costaricensis (red peel, red pulp) is cultivated mainly in Nicaragua, established from clones selected
from the wild. The fruits ripen during the rainy season and
most of the yield is heavily infested with insects, bacteria,
and fungi so that only a small fraction of harvested fruit
can be sold as fresh fruit. Export is mainly to Europe under
the brand name ‘Pitanica.’ Today most of the cultivated
6,000 ha are aimed at pulp production, highly demanded
by the food industry in the United States and Europe as a
natural food ingredient and a colorant (Ortiz 1999; Canto
2000). Guatemala is also producing significant quantities
of this fruit, with several other types of red peel/red pulp
vine pitahayas. Fruits of Hylocereus polyrhizus have a red
peel, as for H. costaricensis, but have a red-violet pulp. The
crop is planted in Israel as a pollinator for H. undatus.
Usually red vine pitahaya orchards consist of 20% H.
polyrhizus and 80% H. undatus. Because of the sour taste,
fruit H. polyrhizus appeals to some consumers more than
that of H. undatus. Selected hybrids produced by inter- or
intra-specific crossings are also cultivated, usually on a local
basis and on a small scale (Mizrahi and Nerd 1999; Ortiz
1999; Canto 2000).
Selenicereus megalanthus
The fruit of Selenicereus megalanthus (yellow peel, white
pulp), often known as “yellow pitaya” but also as pitahaya,
is the most tasty vine cactus fruit (Mizrahi et al. 1997;
Mizrahi and Nerd 1999). The first country to grow this
species for export was Colombia at the request of a
Japanese businessman, who appreciated the fruit.
Significant planting started in Colombia in 1986 and export to Japan started in 1989 but soon ceased due to pests
(eggs and larvae of insects) found where the corolla connects to the fruit. Fruit was then exported to Europe,
which is not as concerned with tropical pests and has become the most important export market for yellow pitaya
(also now shipped from Ecuador via Colombia). Planted
areas in Colombia reached 4,000 ha in 1990 but later were
reduced to 250 ha as result of disease problems, such as
fusarium (which attacks the plant) and especially
Drechslera cactivora (which infects the base of the pre-mature fruit and induces yellowing; Valera et al. 1995;
Bibliowicz and Hernandez 1998). Accessions introduced
into Israel from Colombia, although similar in morphology, vary in fruit characteristics and growth behavior, indicating different genotypes (growers in Colombia do not
keep track of the origin of the material used for planting).
This has been confirmed by DNA finger-printing analysis
developed for vine cacti (Tel-Zur et al. 1999). Fruit of S.
megalanthus obtains prime prices in Europe, higher than
any other cactus fruit, due to its delicious taste (Ortiz 1999;
Canto 2000; J. Rosenbaum, personal communication).
Other yellow-peel vine cacti are available—some clones of
Hylocereus undatus from Mexico and H. costaricensis from
Nicaragua—whose qualities are similar to that of the common red-peel clones of these species. The present sources
of fruit of S. megalanthus shipped to Europe are Colombia,
Ecuador, and Israel.
Stenocereus and Cereus
Stenocereus spp. are cultivated only in Mexico. The fruits
are tasty, resembling those of figs. Usually they are collected and sold in close proximity to where they are harvested
and their price is relatively low (Benz et al. 1997). When
they reach Mexico City, their price can be as high as U.S.
$6 per kilogram. In the 1990s, efforts were directed to converting them into sustainable fruit crops (Pimienta-Barrios
and Nobel 1994; Casas et al. 1997, 1999a,b; PimientaBarrios et al. 1997). The total cultivated area for Stenocereus
spp. in Mexico exceeds 2,000 ha. The main limiting factors
are the short storage and shelf life, in large measure because
Fruits of Vine and Columnar Cacti
193
Figure 11.5. Three-year-old orchard of Cereus peruvianus established near the southern coast of Israel.
of the tendency of the fruit to split during ripening. Cereus
peruvianus is under domestication in Israel (Fig. 11.5) and
is sold in Europe. The fruit lacks spines, has much longer
storage and shelf life than that of Stenocereus, and is similar to that of S. megalanthus. As an index of the acceptability of such new cactus fruits, C. peruvianus was marketed for the first time in 1998 and was accepted very well
in both local and European markets, due to its beautiful
appearance, delicate sour-sweet taste, and unique aroma.
Among eight clones released for cultivation in Israel, only
two have proved to be promising for further planting.
Unlike the others, their fruits do not tend to split upon
ripening or to develop black spots under storage; similar
efforts to develop the cultivation of C. peruvianus exist in
California and Texas.Fgiure5n1.eahrere:
Conclusions and Future Prospects
Pitahayas and pitayas, unlike many other new crops, are
appealing to consumers unfamiliar with the fruit because
of their delicate texture and taste and their unusual and attractive appearance. Slow growth and development of the
plants, low fruit yields, and especially the very short shelf
life of the fruit (about 2 days) limit wide cultivation of
Stenocereus species; commercial plantings occur in their
area of distribution, and fruits are mainly marketed locally by growers and by people collecting fruits from wild
plants. However, pitahayas and Cereus peruvianus are
promising new worldwide crops. They have precocious
194
Nerd, Tel-Zur, and Mizrahi
and high yields and a longer fruit shelf life, which enables
marketing of the fruit over long distances. Indeed, their
cultivation is rapidly spreading around the world, and their
fruits are available in supermarkets and specialty shops.
Pitahayas and pitayas increase the diversity of cultivated fruit crops, which may be used either for sustainable
agriculture systems (e.g., Stenocereus queretaroensis; Fig.
11.2) or for the intensive fruit industry (e.g., Hylocereus undatus; Fig. 11.1). These cacti can compete successfully in
profitability with common fruit crops. Because of the high
water-use efficiency of Crassulacean acid metabolism
(CAM) (Nobel 1994), the low water demand of pitahayas
and pitayas represents a significant advantage in arid and
semiarid regions not only because of the direct water savings but also because the environmental damage caused by
heavily irrigated common crops is avoided. Pitahayas can
also be used as sources for new products, such as colorants
and polysaccharides for the food industry or their edible
flowers.
The recent expansion of fruit crops from vine and
columnar cacti has been based mostly on wild plants from
southern North America (Mexico), central America, and
northern South America (Colombia), where these fruits are
traditionally used by local people. The plants are found
mainly in home gardens or on marginal lands. Thus, professional breeding of improved cultivars should be an important objective, because most of the available material is
a result of superficial selection based on a few visible traits.
Definition of the available plant material by taxonomic,
molecular, and genetic studies is essential for these breeding efforts. Intergeneric and interspecific crosses are easily
achieved among vine cacti, so growers can utilize hybrids
as well as unidentified material as future cultivars. However, the appearance of diverse types of fruits in the markets can lead to confusion and marketing problems. The
lack of distinct common names for existing crops (Table
11.1) already causes confusion for marketing.
Much research is required in order for pitahayas and
pitayas to be established successfully as world crops.
Horticultural treatments as well as orchard management
practices have to be developed. Improved postharvest
treatment is important. Pitahayas growing in structures are
convenient candidates to be fed with CO2; stomata open
for CAM plants during the night when CO2 enrichment
can be done without overheating problems. The dramatic
development of pitahayas and pitayas as fruit crops supports the recent trends to develop ethnic fruits as new
crops and to utilize the underexploited cactus family as a
source of new crops. Unlike some other new crops, a large
gap exists between research and the commercialization of
pitahayas and pitayas—a gap that must be filled for successful cultivation.
Literature Cited
Alvarez, M., S. C. Costa, H. Utumi, A. Huber, R. Ber, and
J. D. Fontana. 1992. The anionic glycan from the cactus Cereus peruvianus—structural features and potential
uses. Applied Biochemistry and Biotechnology 34: 283–
295.
Alvarez, M., S. C. Costa, A. Huber, M. Baron, and J. D.
Fontana. 1995. The cuticle of the cactus Cereus peruvianus as a source of a homo-alpha-D-galacturonan.
Applied Biochemistry and Biotechnology 51: 367–377.
Barbeau, G. 1990. La pitahaya rouge, un nouveau fruit exotique. Fruits 45: 141–147.
Beard, C. E. 1937. Some chromosome complements on the
Cactaceae and a study of meiosis in Echinocereus papillosus. Botanical Gazette 99: 1–21.
Benz, B. F., F. M. Santana, J. E. Cevallos, E. M. Munoz,
J. A. Rosales, and M. A. Rosales. 1997. The structure
and productivity of relict stands of pitaya (Stenocereus
queretaroensis; Cactaceae), Jalisco, Mexico. Economic
Botany 51: 134–143.
Bibliowicz, A., and S. M. Hernandez. 1998. Organismos
Fungosos Presentes en las Estructuras Reproductivas de la
Pitaya Amarilla. Universidad Nacional de Colombia,
Santafé de Bogotá.
Britton, N. L., and J. N. Rose. 1963. The Cactaceae:
Description and Illustrations of Plants of the Cactus
Family. Vols. I & II. Dover Publications, New York.
Cacioppo, O. G. 1990. Pitaya: una de las mejores frutas
producida por Colombia. Informative Agroeconómico,
Febrero: 15–19.
Canto, A. R. 2000. Pitahayas. Estado Mundial de su Cultivo
y Comercialización. Fundacion Yucatan Produce,
Universidad Autónoma, Chapingo, Mexico.
Casas, A., B. Pickersgill, J. Caballero, and A. ValienteBanuet. 1997. Ethnobotany and domestication in
Xoconochtli, Stenocereus stellatus (Cactaceae), in the
Tehuacan Valley and La Mixteca Baja, Mexico.
Economic Botany 51: 279–292.
Casas, A., J. Caballero, A. Valiente-Banuet, J. A. Soriano,
and P. Davila. 1999a. Morphological variation and the
process of domestication of Stenocereus stellatus
(Cactaceae) in central Mexico. American Journal of
Botany 86: 522–533.
Casas, A., A. Valiente-Banuet, A. Rojas-Martinez, and P.
Davila. 1999b. Reproductive biology and the process of
domestication of the columnar cactus Stenocereus stellatus in central Mexico. American Journal of Botany 86:
534–542.
Deoliveira, S. A., M. D. P. D. Machado, A. J. Prioli, and
C. A. Mangolin. 1995. In-vitro propagation of Cereus
peruvianus Mill (Cactaceae). In Vitro Cellular and
Developmental Biology—Plant 31: 47–50.
Feng-Ru, C., and Y. Chung-Ruey. 1997a. Flowering and
fruit growth of pitaya (Hylocereus undatus Britt. &
Rose). Journal of the Chinese Society of Horticultural
Science 43: 314–321 (Chinese with English summary).
Feng-Ru, C., and Y. Chung-Ruey. 1997b. Forcing pitaya
(Hylocereus undatus Britt. & Rose) by chemicals and
controlled daylength and temperature. In Proceedings
of the Symposium on Enhancing Competiveness of the
Fruit Industry. Taipei, Taiwan. Pp. 163–190. (Chinese
with English summary.)
Gibson, A. C., and P. S. Nobel. 1986. The Cactus Primer.
Harvard University Press, Cambridge, Massachusetts.
Haber, W. A. 1983. Hylocereus costaricensis (pitahaya
sylvestre, wild pitahaya). In Costa Rican Natural History (D. H. Janjen, ed.). University of Chicago Press,
Chicago. Pp. 252–253.
Infante, R. 1992. In vitro axillary bud proliferation and somatic embryogenesis of yellow pitaya Mediocactus coccineus (Salm-Dyck). Plant Cell, Tissue, and Organ
Culture 31: 155–159.
Fruits of Vine and Columnar Cacti
195
IOS. 1986. The genera of the Cactaceae: Towards a new
consensus. Bradleya 4: 65–78.
Lichtenzveig, J., S. Abbo, A. Nerd, N. Tel-Zur, and Y.
Mizrahi. 2000. Cytology and mating systems in the
climbing cacti Hylocereus and Selenicereus. American
Journal of Botany 87: 1058–1065.
Machado, M. D. P. S., and A. J. Prioli. 1996. Micropropagation of Cereus peruvianus Mill (Cactaceae) by
areole activation. In Vitro Cellular and Developmental
Biology—Plant 32: 199–203.
Metz, C., A. Nerd, and Y. Mizrahi. 2000. Viability of
pollen of two fruit cacti of the genus is affected by temperature and duration of storage. HortScience 35: 22–
24.
Mizrahi, Y., A. Nerd, and P. S. Nobel. 1997. Cacti as crops.
Horticultural Reviews 18: 291–320.
Mizrahi, Y., and A. Nerd. 1999. Climbing and columnar
cacti: New arid land fruit crops. In Perspectives on New
Crops and New Uses ( J. Janick, ed.). American Society
of Horticultural Science, Alexandria, Virginia. Pp.
358–366.
Moran, V. C. 1953. Selenicereus megalanthus (Schumann)
Moran. Gentes Herbarum 8: 325.
Moran, V. C., and H. G. Zimmerman. 1991. Biological
control of cactus weeds of minor importance in South
Africa. Agriculture, Ecosystems & Environment 37: 37–
55.
Nerd, A., and Y. Mizrahi. 1997. Reproductive biology of
cactus fruit crops. Horticultural Reviews 18: 321–346.
Nerd, A., and Y. Mizrahi. 1998. Fruit development and
ripening in yellow pitaya. Journal of the American
Society of Horticultural Science 123: 560–562.
Nerd, A., and Y. Mizrahi. 1999. The effect of ripening stage
on fruit quality after storage in yellow pitaya. Postharvest Biology and Technology 15: 99–105.
Nerd, A., E. Raveh, and Y. Mizrahi. 1993. Adaptation of
five columnar cactus species to various conditions in
the Negev Desert of Israel. Economic Botany 47: 304–
311.
Nerd, A., F. Gutman, and Y. Mizrahi. 1999. Ripening and
postharvest behavior of fruits of two Hylocereus species
(Cactaceae). Postharvest Biology and Technology 17: 39–
45.
196
Nobel, P. S., and E. Pimienta-Barrios. 1995. Monthly stem
elongation for Stenocereus queretaroensis—relationships
to environmental conditions, net CO2 uptake, and seasonal variations in sugar content. Environmental and
Experimental Botany 35: 17–24.
Ortiz, Y. H. 1999. Pitahaya, a New Crop for Mexico.
Editorial Limusa, Mexico City.
Pimienta-Barrios, E. 1999. El Pitayo en Jalisco y Especies
Afines en México. Universidad de Guadalajara,
Zapopan, Mexico.
Pimienta-Barrios, E., and P. S. Nobel. 1994. Pitaya (Stenocereus spp. Cactaceae)—An ancient and modern fruit
crop of Mexico. Economic Botany 48: 76–83.
Pimienta-Barrios, E., and P. S. Nobel. 1995. Reproductive
characteristics of Pitayo (Stenocereus queretaroensis)
and their relationship with soluble sugars and irrigation. Journal of the American Society of Horticultural
Science 120: 1082–1086.
Pimienta-Barrios, E., P. S. Nobel, C. Robles-Murguia, L.
Mendez-Moran, E. Pimienta-Barrios, and E. YepezGonzalez. 1997. Ethnobotany, productivity, and ecophysiology of pitaya (Stenocereus queretaroensis).
Journal of the Professional Association for Cactus
Development 2: 29–47.
Raveh, E., M. Gersani, and P. S. Nobel. 1995. CO2 uptake
and fluorescence responses for a shade tolerant cactus
Hylocereus undatus under current and doubled CO2
concentrations. Physiologia Plantarum 93: 505–511.
Raveh, E., A. Nerd, and Y. Mizrahi. 1997. Responses of
climbing cacti to different levels of shade and to carbon
dioxide enrichment. Acta Horticulturae 434: 271–278.
Raveh, E., A. Nerd, and Y. Mizrahi. 1998. Responses of two
hemiepiphytic fruit crop cacti to different degrees of
shade. Scientia Horticulturae 53: 115–122.
Silva, W. R., and M. Sazima. 1995. Hawkmoth pollination
in Cereus peruvianus, a columnar cactus from Southeastern Brazil. Flora 190: 339–343.
Silvius, K. M. 1995. Avian consumers of cardon fruits
(Stenocereus griseus, Cactaceae) on Margarita Island,
Venezuela. Biotropica 27: 96–105.
Spencer, J. L. 1955. A cytology study of the Cactaceae of
Puerto Rico. Botanical Gazette 117: 33–37.
Nobel, P. S. 1988. Environmental Biology of Agaves and
Cacti. Cambridge University Press, New York.
Taylor, N. P., and D.C. Zappi. 1992. Proposal to conserve
Cereus jamacaru (Cactaceae) with a new type. Taxon 41:
590–591.
Nobel, P. S. 1994. Remarkable Agaves and Cacti. Oxford
University Press, New York.
Tel-Zur, N., S. Abbo, D. Myslabodski, and Y. Mizrahi.
1999. Modified CTAB procedure for DNA isolation
Nerd, Tel-Zur, and Mizrahi
from epiphytic cacti of the genera Hylocereus and
Selenicereus (Cactaceae). Plant Molecular Biology
Reporter 17: 249–254.
Varela, C. H., De. P. M. Sanchez, and N. Bravo. 1995.
Etiological study of the “dry leaf rot” disease in yellow
pitaya (Selenicereus megalanthus). Fitopatologia Colombiana 19: 40–47.
Wang, X. 1997. Biology of ripening of apple cactus (Cereus
peruvianus [L.] Miller). Ph.D Thesis, Ben Gurion
University of the Negev, Beer-Sheva, Israel.
Weiss J., A. Nerd, and Y. Mizrahi. 1994a. Flowering behavior and pollination requirements in climbing cacti
with fruit crop potential. HortScience 29: 1487–1492.
Weiss J., A. Nerd, and Y. Mizrahi. 1994b. Flowering and
pollination requirements in Cereus peruvianus cultivated in Israel. Israel Journal of Plant Sciences 42: 149–
158.
Weiss, J., L. Scheinvar, and Y. Mizrahi. 1995. Selenicereus
megalanthus (the yellow pitaya), a climbing cactus from
Colombia and Peru. Cactus and Succulent Journal
(U.S.) 67: 280–283.
Wu, M. C., and C. S. Chen. 1997. Variation of sugar content in various parts of pitaya fruit. Proceedings of the
Florida State Horticultural Science 110: 225–227.
Fruits of Vine and Columnar Cacti
197
CHAPTER
› 12 ‹
F O R AG E , F O D D E R ,
AND ANIMAL NUTRITION
Ali Nefzaoui and Hichem Ben Salem
Introduction
Increasing Usage of Cacti in Arid Zones
Use of Cacti as Fodder
Nutrient Contents
Intake and Digestibility
Cladode Consumption and Sheep Rumen Fermentation
Cladodes as a Water Source
Some Practical Considerations
Integration of Platyopuntias with Other Feed Resources in the WANA Region
Conclusions
Literature Cited
Introduction
The future of arid and semiarid regions depends on the development of sustainable agricultural systems and on the
cultivation of appropriate crops. Such crops must successfully withstand water shortage, high temperature, and poor
soil fertility. Cacti, particularly platyopuntias, can satisfy
these requirements and are becoming increasingly important for both subsistence and market-oriented activities in
arid and semiarid regions (Barbera 1995), as for vertebrates
in natural ecosystems (Chapter 7). This chapter focuses on
the West Asia/North Africa (WANA) region, large portions of which have winters with low and erratic rainfall
and hot, dry summers. It is characterized by high population growth, limited areas of arable land, harsh deserts, and
limited water resources for irrigation development
(Nordblom and Shomo 1995). The WANA region contains
most of the more than 1 million hectares (ha) utilized
worldwide (Table 12.1) for raising platyopuntias—principally Opuntia ficus-indica — for forage (consumption in
situ) and fodder (consumption after harvest and transport)
for livestock
As much as 50% of arid rangeland in the WANA region may have lost its vegetation since World War II, while
concomitantly the human population has increased fourfold (Le Houérou 1991). In this regard, the sheep population has increased by 75% and the stocking rate has
jumped from one sheep per 4 ha to one sheep per ha between 1950 and 1989. Indeed, the contribution of such
rangelands to the annual feed requirements of livestock is
in decline, going from 80% in 1965 to less than 25% of the
requirement in 2000. Overgrazing and range deterioration
have forced pastoralists to change their migration and feeding patterns. In some countries, the forage diet of animals
is heavily supplemented with barley grain and other commercial concentrated feeds. A typical feed calendar for
199
TA B L E 1 2 . 1
Estimated land areas utilized for raising cacti, mainly Opuntia ficusindica, for forage and fodder
Region or country
Land area utilized (ha)
Brazil
Other South American countries
Mexico
Other North American countries
Southern Africa
WANA region
Tunisia
Algeria
Other WANA countries
(Morocco, Libya, Egypt,
Jordan, Syria, Lebanon)
370,000
75,000
230,000
16,000
2,000
tion should not have an adverse effect on the plants, i.e.,
the plants must have high recovery ability; (5) establishment and maintenance of the plantations must be cost
effective and should have a low initial cost; and (6) the
crop must be relatively undemanding with respect to soil
and climatic requirements. Using these criteria, Opuntia
ficus-indica has proved to be an important fodder crop in
Tunisia, such as for feeding sheep (Fig. 12.1).Fgiure12n.eahrere:
Increasing Usage of Cacti in Arid Zones
500,000
150,000
300,000
Data are from Le Houérou (1992), Nobel (1994), and various
Ministry of Agriculture reports.
agropastoral systems of arid and semiarid regions of the
WANA region also includes supplementation with cactus
in May through September (Table 12.2).Tabe12l.neahrere:
The search for plant species with the ability to grow
and produce in arid areas has been a permanent concern in
most WANA countries. The characteristics of cacti fit
most of the requirements of a drought-resistant fodder
crop, as described by De Kock (1980): (1) the crop must
not only be able to withstand long droughts, but it must
also be able to produce large quantities of fodder during
periods of favorable rainfall that can be utilized during
drought periods; (2) the crop must have a high carrying capacity; (3) the crop must not have an adverse effect on the
health of the animals consuming it; (4) extensive utiliza-
Cacti grow in “deserts” and are drought tolerant. Indeed,
they possess a specialized photosynthetic mechanism,
which leads to a more efficient production of dry matter
per unit water consumed than that of grasses or legumes
(Russell and Felker 1987; Nobel 1988, 1989; Chapter 4).
Cacti produce fodder, fruit, and other useful products.
They also can prevent the long-term degradation of ecologically weak environments. Cacti in general and Opuntia
spp. in particular were introduced into the WANA region
by Spanish moors in the 16th century (Le Houérou 1992).
Nevertheless, only toward the end of the 20th century have
large plantations been established. These create evergreen
fodder banks to feed animals during drought and combat
desertification as well.
Opuntia spp. used for animal feeding are abundant,
easy and cheap to grow, palatable, and able to withstand
prolonged droughts (Shoop et al. 1977). Such characteristics make them a potentially important feed supplement
for livestock, particularly during periods of drought and
seasons of low feed availability. The cladodes constitute the
majority of the biomass of a platyopuntia and can be fed
to livestock as fresh forage or stored as silage for later feeding (Castra et al. 1977). In any case, the idea of using cactus to feed livestock is not recent. Griffith (1905) confirmed
TA B L E 1 2 . 2
Common feed calendar for small ruminants in the WANA region
Months
200
Physiological stage
Area
Main type of feed
Supplement
May–July
Mating,
early pregnancy
Agricultural land
Cereal stubble
Bran, barley, cactus
August–September
Pregnancy
Agricultural land
Cereal stubble,
straw
Bran, barley, cactus,
shrubs (Atriplex)
October–January
Late pregnancy,
early lactation
Rangeland,
agricultural land
Fallow, hay,
natural grazing
Barley, wheat, bran, olive
tree leaves and twigs
February–April
Weaning, fattening
Rangeland,
agricultural land
Natural grazing,
fallow, standing
barley, straw
Olive tree leaves and
twigs, barley, bran
Nefzaoui and Ben Salem
yield
(tons fresh weight ha -1 year -1 )
A
120
100
80
60
40
20
0
0
100
200
300
400
500
600
rainfall (mm year -1 )
B
C
Figure 12.1. Use of Opuntia ficus-indica for fodder in Tunisia:
(A) Transport of cladodes from the field; (B) chopping of cladodes,
which can be laborious; and (C) feeding chopped cladodes to sheep.
that feeding platyopuntias to livestock started in the
United States before the Civil War (1861–1865). Cladodes
were extensively transported from regions of Texas, such as
Brownsville, Indianola, San Antonio, and Eagle Pass. Both
natural and cultivated populations of platyopuntias have
become important for fodder in many parts of the world.
They are cultivated in Africa, Argentina, Bolivia, Brazil,
Chile, Colombia, Israel, Italy, Mexico, Peru, Spain, and the
United States (Curtis 1979; Le Houérou 1979; Brutsh 1984;
Russell and Felker 1987; Clovis de Andrade 1990; Barbera
et al. 1992; Flores Valdez and Aguirre Rivera 1992).
Figure 12.2. Productivity of spineless cactus (Opuntia ficus-indica var.
inermis) versus rainfall in Tunisia. Adapated from Monjauze and Le
Houérou (1965).
Large areas of platyopuntias are available year-round in
Algeria, Mexico, and especially Brazil and Tunisia and serve
as an emergency feedstock in case of drought (Table 12.1).
In many arid areas (e.g., Tunisia, Mexico, South Texas,
South Africa), farmers make extensive use of cacti as emergency forage, harvesting it from both wild and cultivated
populations to prevent the disastrous consequences of frequent and severe droughts (Le Houérou 1992). Since 1900
in North Africa, shrubs (mainly Acacia cyanophylla and
Atriplex nummularia or A. halimus) and cacti (mainly
Opuntia ficus-indica var. inermis) have been planted to reduce water and wind erosion and rangeland degradation.
Nearly 1 million ha have been planted in Algeria, Morocco,
Libya, and Tunisia (Table 12.1). These plantations in low
rainfall areas also provide feed for livestock during frequent
drought periods. Because of their high water-use efficiency
(Nobel 1988, 1989), the aboveground productivity of cacti
tends to be much higher than that of most other plant
species in arid and semiarid regions. For example, under
rain-fed conditions in Tunisia and with no fertilizer application, spineless platyopuntia yields vary from 20 to 100
tons fresh weight of cladodes ha-1 year-1 as rainfalls increase
from 150 to 400 mm per year (Fig. 12.2).Fgiure12.neahrere:
Marginal lands often represent fragile ecosystems.
Ploughing and indiscriminate vegetation removal can result
in large-scale degradation and destruction of vegetation
cover. The scarcity and even disappearance of several plant
species indicate the magnitude of genetic and edaphic losses (Le Houérou 1991). To reverse the desertification trend
and to restore vegetation cover in such areas, appropriate
integrated programs are needed for rangeland monitoring,
livestock control, and conservation of natural resources.
Forage, Fodder, and Animal Nutrition
201
Figure 12.3. Use of Opuntia ficus-indica to consolidate water harvesting along terraces in Tunisia.
Spineless cactus (O. ficus-indica), which is drought and erosion tolerant, is being advantageously and widely established in Tunisia and Algeria to slow and direct sand movement, to enhance the restoration of the vegetation cover,
and to avoid the destruction by water of the land terraces
built to reduce runoff (Fig. 12.3). In central and southern
Tunisia, cactus plantations play a key role in natural resources conservation as well as provide a large amount of
fodder for livestock. The strong rooting system of cacti
helps stabilize land terraces. One or two rows of cladodes
are planted on the inner side of the terraces (Fig. 12.3). The
rooting system is enhanced by the collection of the water
at the base of the terrace. Roots are widespread on the elevated part of the terrace and go deeply into the soil, ensuring stability of the terraces. As an additional benefit, cut
and carried cladodes from such plantings provide sufficient
feed resources during dry spells. Platyopuntias are also used
in combination with cement barriers or cut palm fronds to
stop wind erosion and sand movement.Fgiure123n.eahrere:
Use of Cacti as Fodder
Nutrient Contents
Platyopuntias are an unbalanced feed nutrientwise but a
cost-effective source of energy and water. Cladodes are low
in crude protein, fiber, phosphorus, and sodium (Le
Houérou 1992; Nefzaoui et al. 1995). Therefore, diets containing cactus should be balanced for these nutrients by appropriate supplements. The water content on a freshweight basis averages nearly 90% (Table 12.3). The ash
content is about 20% of the dry weight. The crude protein
is often below 5% but can be up to 10% of the dry weight
(Table 12.3). The fiber content is also relatively low, about
10% of the dry weight. The nitrogen-free extract, which includes monomeric and polymeric sugars, is about 60% of
202
Nefzaoui and Ben Salem
the dry weight (Table 12.3). Cladodes’ low content of phosphorous (P; about 0.03% of the dry weight) and sodium
(Na; about 0.01%) requires supplementation when they
are fed to animals.Tabe12l3n.eahrere:
The ash content of cladodes is high (Table 12.3),
mainly because of the high calcium (Ca) content. High Ca
levels in soils in arid and semiarid regions cause cacti to accumulate high quantities of Ca compounds (Nobel 1988).
Platyopuntia cladodes also have high levels of oxalates (Ben
Salem et al. 2002). For example, the oxalate amount is
about 13% of the dry weight and 40% is in soluble form
(the high amount of oxalates may explain the laxative effect
of cladodes when fed to animals). The oxalates mostly contain Ca, making this cation less available to animals.
Nevertheless, the Ca content of cladodes is generally higher than animal requirements. An excess of Ca is not a problem per se, but an unbalanced Ca:P ratio, which is generally about 35 for cladodes, needs to be corrected. Indeed,
Shoop et al. (1977), working with Opuntia polyacantha, indicated that its P content is below livestock dietary requirements. In particular, Ca levels are adequate but the
Ca:P ratio of about 36 is too high for optimal livestock performance. De Kock and Aucamp (1970) reported the same
problem with O. ficus-indica and suggested using licks for
P and Na. These licks may be made from the following ingredients: salt, monosodium phosphate, molasses, dicalcium phosphate, and bonemeal. According to Shoop et al.
(1977), most other minerals in cladodes (e.g., manganese,
copper, zinc, magnesium, and iron) have concentrations
within the range generally acceptable in the diets of ruminants. An exception is sodium content, which is relatively
low (0.02% for O. polyacantha).
Crude fiber content (Table 12.3) is actually a poor indicator of feed fiber quality. For instance, the fibers cellulose and lignin have low digestibility. For five platyopuntias (Opuntia engelmannii, O. filipendula, O. fragilis, O.
polyacantha, and O. versicolor), cellulose averages 11 ± 2%
(mean ± 1 standard deviation) of the dry weight, hemicellulose averages 18 ± 3%, and lignin averages 3.9 ± 0.7%
(Ben Thlija 1987); in comparison, for alfalfa these values
are 22%, 15%, and 7.9%, respectively.
Although the protein content of cladodes is generally
low (Table 12.3), it tends to increase after nitrogen fertilization. For instance, N and P fertilizers can increase
crude protein contents of Opuntia cladodes from 4.5 to
10.5% of the dry weight (Gonzalez 1989). Also, Gregory
and Felker (1992) found that some clones from Brazil have
over 11% crude protein. In the WANA region, large plantations of Opuntia are utilized for fodder production in
dry areas where soil quality, rainfall, and finances argue
TA B L E 1 2 . 3
Mean chemical composition of platyopuntia cladodes used for forage or fodder
Species
Opuntia engelmannii,
O. lindhymeri a
O. ficus-indica
variousb
Californiac
Chilec
Tunisiad
Water
(% of
fresh weight)
Ash
Crude
fiber
N-free
extract
Ca
Mg
P
K
8.3
1.6
0.04
3.0
6.3
3.9
8.7
1.4
1.3
0.033
0.012
0.04
1.2
2.0
1.1
Na
(% of dry weight)
85
89
90
89
87
Crude
protein
2.9
17
27
4.8
10.4
8.9
3.8
10.9
8.6
65
64
58
0.0031
0.0030
0.05
a Hoffman
and Walker (1912)
(1958); De Kock (1965); Theriez (1965); Teles (1978)
c Nobel (1988)
d A. Nefzaoui (unpublished observations)
b Lozano
against fertilizer application. Therefore, increasing the nitrogen content of cladodes through selection or hybridization is more promising. Protein deficiency can also
be solved through appropriate supplementation and/or
feed combinations. Wehren (1976) showed that the amino
acid composition of cladodes of O. ficus-indica var. inermis is quite satisfactory for animals; the proteins of three
cultivars contained an average of 10.6% aspartic acid,
13.0% glutamic acid, 4.3% threonine, 4.3% serine, 6.5%
proline, 4.8% glycine, 7.8% alanine, 0.8% cystine, 7.0%
valine, 2.1% methionine, 5.2% isoleucine, 8.3% leucine,
4.1% tyrosine, 5.1% phenylalanine, 5.9% lysine, 2.3% histidine, and 5.5% arginine.
The nutritive quality of Opuntia cladodes depends on
plant type (species, varieties), cladode age, season, and
agronomic conditions (e.g., soil type, climate, and growing
conditions). In Tunisia, the percentage dry weight is highest and crude protein content is lowest during the summer
season; the ash content tends to be highest in the spring
and crude fiber content highest in the winter (Ben Salem
et al. 1996b). The percentage dry weight increases as
cladodes age. Also, when cladode age increases from 1 to 5
years, crude protein content of O. ficus-indica var. inermins
decreases (from 5 to 3% of dry weight) and crude fiber increases (from 9 to 20%; A. Nefzaoui, unpublished observations). This trend is similar to that for other fodder
sources, where nutrients decrease with plant age resulting
from the relative increase in fiber content (Nefzaoui and
Ben Salem 1998).
Intake and Digestibility
Animals can consume large amounts of cladodes. For instance, Jersey cows fed on cladodes and supplemented with
1 kg day-1 of concentrated feed consume 51 kg fresh weight
day-1 of the cladodes (Woodward et al. 1915). Metral
(1965) similarly found a voluntary intake of 60 kg day-1
when cladodes alone are fed to cows. Viana (1965) reported an average voluntary intake of 77 kg day-1 for cows. For
sheep, Monjauze and Le Houérou (1965) reported cladode
intakes of 2.5 to 9 kg day-1. Valdez and Flores (1967) observed higher intake with O. ficus-indica (11 kg day-1) than
with O. robusta (6.5 kg day-1). Intakes are higher when the
water content of the cladodes is high (A. Nefzaoui and H.
Ben Salem, unpublished observations). Cladode consumption can have a laxative effect on sheep, leading to a
more rapid passage of the food through the animal’s digestive tract. This leads to poorer digestion, especially
when the cladodes constitute more than 60% of the dry
matter intake; supplementing with fibrous feed (e.g., straw
or hay) can alleviate such laxative effects.
Sheep fed straw consume up to 600 g dry weight of
cladodes without any digestive disturbance (Cordier 1947).
Supplementation of poor quality roughages with molasses
increased their palatability (Preston and Leng 1987;
Rangnekar 1988), so cladodes with their high carbohydrate
contents may have similar effects as molasses. The energy
content of cladodes is 3,500 to 4,000 kcal kg-1 dry weight,
just over half of which is digestible and comes mainly from
Forage, Fodder, and Animal Nutrition
203
TA B L E 1 2 . 4
Effect of supply of spineless Opuntia ficus-indica var. inermis on intake and digestibilities by sheep fed strawbased diets in Tunisia
Amount of cladodes (g dry weight day –1)
Dry weight intake (g day –1)
Straw
Cactus + straw
Total digestibility
Organic matter
Crude protein
Crude fiber
Digestible intakes (% of
maintenance requirements)
Organic matter
Crude protein
Protozoa (104 per ml)
Degradability of cellulose (%)
0
150
300
450
600
550a
550a
574ab
724b
523a
823c
643bc
1,093d
716c
1,278e
45a
50a
53a
50ab
55ab
51a
54b
54ab
53a
58b
59bc
52a
59b
64c
47a
123
52
9.3b
72
158
64
13.0c
57
193
93
17.7d
55
212
111
13.1c
56
93
52
3.5a
85
Means in the same row followed by different letters differ (P < 0.05). Data are from Ben Salem et al. (1996).
carbohydrates (De Kock 1983; Ben Thlija 1987). In arid
and semiarid regions of North Africa, cereal crop residues
and natural pastures generally do not meet the nutrient requirements of small ruminants for meat production.
Cladodes can provide a cost-effective supplementation,
such as for raising sheep and goats on rangelands. For instance, when diets of grazing sheep are supplemented with
cladode cakes, the daily weight gain increases nearly 50%
(to 145 g day-1; Tien et al. 1993); when cladodes are supplied to grazing goats that have access to alfalfa hay, the
milk yield is increased by 45% (to 436 g day-1; Azocar et
al. 1991). When cladodes are associated with a protein-rich
feedstuff, they may replace barley grains (Ben Salem et al.
1998) or maize silage (Metral 1965) without affecting
sheep and cattle daily weight gains. For instance, milk
yield for lactating goats supplied with 2.2 kg alfalfa hay
day-1 is actually slightly higher (1,080 g day-1) when 0.7 kg
cladodes replaces an equal mass of alfalfa (Azocar et al.
1996).
When sheep are offered increasing amounts of cladodes, their intake of the accompanying straw also increases (Table 12.4). The dry matter intake of straw plus cladodes steadily increases as the amount of cladodes supplied
increases. The cladodes are highly digestible, with similar
digestibilities for organic matter, crude protein, and crude
fiber, ranging from 45 to 64% (Table 12.4). Digestibility of
cladodes by sheep is similar to that for common forage
204
Nefzaoui and Ben Salem
crops. The main difference between platyopuntias and
other forage crops is the timing of the degradability of nutrients in the rumen. For other forage crops, maximal
degradability in the rumen requires about 48 hours, whereas nutrients in cladodes are degraded very rapidly (between
6 and 12 hours) and little additional nutrient extraction occurs after 24 hours (Ben Thlija 1987). Similarly, 80% of the
total digestion of singed Great Plains prickly pear (Opuntia
polyacantha) by cattle occurs during the first 16 hours
(Shoop et al. 1977).Tabe12l4.neahrere:
A rapid rate of digestion leads to a faster passage of the
material through the digestive tract, leading to more available volume for further intake. Because of the low gut fill
of cladodes, an increase of cactus in the diet does not necessarily reduce the intake of other components of the ration (Table 12.4). This is of great importance for arid zones
where livestock is fed mainly with straw or cereal stubble,
which are low quality coarse feeds that have poor intakes,
resulting in low animal daily weight gains. In feeding trials with heifers in the United States, cladodes are more
readily and more completely digested than is grass-hay
(Agropyron cristatum and Bromus spp.). In South Africa,
the yields (tons fresh weight ha-1 year-1) and digestible nutrients (tons fresh weight ha-1 year-1) can be 80 and 5.0 for
O. ficus-indica, 25 and 4.2 for Zea mays (maize silage), and
5 and 2.5 for dried lucerne hay (Rossouw 1961), suggesting
a superiority of the platyopuntia as a forage.
Ben Salem et al. (1996a) studied the effect of increasing the
level of spineless cladodes on rumen fermentation in
sheep given straw ad libitum. The cactus improves rumen
fermentation (Table 12.4), as also occurs for sheep on an
Acacia cyanophylla–based diet supplemented with cladodes
and urea (Ben Salem 1998). In both cases the rumen fluid
pH is not affected by up to 600 g dry weight of cladodes
day-1, remaining at 6.9 ± 0.1. The cladodes are rich in easily fermentable carbohydrates, and their consumption
probably enhances salivation.
Compared with a cactus-free diet, the highest supply of
cladodes doubles the concentration of ammonia nitrogen
in the rumen of sheep fed diets based on straw (Ben Salem
et al. 1996a) or acacia (Ben Salem 1998). This leads to ammonia concentrations near optimal for microbial growth
and fiber digestion in the rumen (Satter and Slyter 1974;
Ushida and Jouany 1985). Indeed, the protozoa counts per
volume increases fourfold as the amount of cladodes ingested exceeds 300 g dry weight day-1 (Table 12.4). Adding
cladodes to the diet can increase the volatile fatty acids
(such as propionic acid) by up to 30%, reflecting the increased intake of soluble carbohydrates.
Ben Salem et al. (1996a) used an in sacco technique
(Ørskov et al. 1980) to study the effect of cladode supply
on cellulolytic activity in the rumen of sheep on a strawbased diet. Nylon bags (pore size of 46 µm) are filled with
specified amounts of cladodes and straw, incubated in the
rumen for various times, and then removed and the dry
weights of the various fractions determined. The degradability of cellulose is adversely affected by increasing
amounts of cladodes in the diet (Table 12.4), although the
rate of degradation is not affected (Ben Salem et al.
1996a). The impairment of cellulolytic activity in the
rumen as the cladode intake increases may be because the
increasing ciliate protozoa have a negative effect on the
number of bacteria in the rumen and thus on cellulose
degradation (Demeyer and Van Nevel 1979). The high
level of minerals in cladodes may also decrease microbial
growth in the rumen (Komisarczuk-Bony and Durand
1991). In any case, a combination of O. ficus-indica and cereal straw is a nutritionally satisfactory solution for maintaining small ruminants in arid regions. Indeed, supplying
cladodes can improve the nutritive value and intake of the
poor quality roughages.
Cladodes as a Water Source
Water scarcity can depress feed intake, digestion, and
therefore weight gains of sheep and goats (Wilson 1970; El-
water intake (liter day -1 )
3
Cladode Consumption and Sheep Rumen Fermentation
2
1
0
0
200
400
600
cladode intake (g dry weight day -1 )
Figure 12.4. Influence of cladode consumption on daily water uptake
by sheep. Data are from Ben Salem et al. (1996).
Nouty et al. 1988; Silanikove 1992). Thus, supplying livestock with water during the summer and during drought
periods is crucial in the WANA region and other arid regions. Animals consume considerable energy to reach
water points. Moreover, rangeland degradation in the area
surrounding water points is a serious problem. Therefore,
the high water content of cladodes is beneficial in dry
areas. Cattle given abundant supplies of cladodes require
little (Cottier 1934) or no (Woodward et al. 1915) additional
water. Sheep fed for a long period (400 to 500 days) with
large amounts of cladodes stop drinking (Roussow 1961;
Harvard-Duclos 1969). Also, water intake by sheep on a
wheat straw diet becomes extremely low when the daily intake of cladodes is 300 g dry weight day-1 or more (Fig.
12.4), consistent with other findings (Terblanche et al.
1971). Similarly, sheep on an Acacia cyanophylla diet require
1.2 liter water day-1 when supplemented with barley but
only 0.6 liter day-1 when supplemented with cladodes (Ben
Salem et al. 2002).Fgiure124.neahrere:
Some Practical Considerations
The method of utilization of cladodes for livestock will
differ according to circumstances, such as available labor,
facilities, and quantity of cactus available. Although grazing of cladodes in situ is the simplest method, it is not the
most efficient and care should be taken so that the animals
do not overgraze the plants. The harvested cladodes may be
cut into small pieces or strips and fed in a confined area to
limit wastage (Fig. 12.1C). The cladodes can also be cut
into smaller pieces and mixed with hay or low-quality alfalfa to make silage (if no fruits are included, molasses is
usually added), which is maintained anaerobically.
Forage, Fodder, and Animal Nutrition
205
Cladodes fed in various forms can keep animals alive for
long periods. To supplement cladodes fed ad libitum, alfalfa or hay can provide protein and a lick of equal parts by
mass of bone meal, salt, and fodder lime can provide phosphate and sodium (De Kock 1983).
It is cheapest to store cladodes as parts of living plants
instead of after harvesting. Once harvested, chopped
cladodes can be dried and then ground, e.g., using a hammermill with a sieve having 6-mm-diameter pores. Cladodes in the form of meal are not only more easily ingested
but also are easier to store for use during droughts. Good
quality silage can be made by chopping the pads together
with oat straw, low-grade lucerne hay, or other roughage
using 84 parts by mass of cladodes and 16 of roughage with
the addition of 2% molasses.
The easiest and cheapest way to utilize platyopuntias is
grazing. However, overgrazing of the plantation must be
guarded against. Young plants are especially susceptible to
overgrazing and can be killed by sheep. Even older plants
can be so badly damaged that the subsequent production
will be considerably lower. The best method for grazing is
to divide the plantation into small paddocks and to graze
each of these intensively for a few months every 3 years or
so. Direct browsing necessitates a very tight control on
grazing, otherwise wastage may reach 50% of the fodder
produced (cladodes partially eaten and abandoned) and
the plantation itself may be destroyed by overbrowsing
(Monjauze and Le Houérou 1965; De Kock 1980). This
type of management has a very low cost and the grass between the shrubs is available to the stock. Plants in a paddock can be grazed to one cladode higher than the original planting so that the plants recover well, the material
used is of good quality, and the plants are kept within a usable size. Zero grazing—the cut-and-carry technique—has
the opposite consequences. Loss of feed is virtually nil and
risk of overutilization is small, except when young plantations are harvested too early. Even though the zero-grazing
technique is labor-intensive, in most cases in North Africa
it is the recommended method because of insufficient grazing discipline.
Although spineless O. ficus-indica is easiest to use,
cladodes with spines are also valuable feed provided the
spines are singed off first, usually through the use of a
propane burner (Shoop et al. 1977). In Texas and Mexico,
the standing plant is singed, whereas in North Africa detached cladodes are singed and then chopped into small
pieces by hand (Fig. 12.1B) or machines. Steaming to
moisten the spines and chopping of large pads can also facilitate the consumption of cladodes by livestock (Griffiths
1905).
206
Nefzaoui and Ben Salem
Integration of Platyopuntias with
Other Feed Resources in the WANA Region
In the WANA region climatic conditions lead to two feed
gaps each year (Table 12.1): (1) in the winter (2–4 months)
and (2) an even larger one in the summer plus autumn (5–
6 months). These gaps require large amounts of supplemental feed. National strategies aimed to increase rangeland productivity have focused on highly producing
species, such as Acacia cyanophylla, Atriplex nummularia
(or A. halimus), and spineless platyopuntias. Several techniques for planting shrubs and cacti are used: (1) on communal rangelands, where introduced species are planted in
rows without removal of natural herbaceous or woody natural species; (2) on private land an alley-cropping technique is preferred, where farmers can cultivate the area between rows when the rainfall conditions are favorable; (3)
when soil conservation is crucial (Fig. 12.3), plantings are
done for both types following the contour lines; and (4)
the oldest type (“bosquet”) involves a dense planting of
platyopuntias around the house for fruit and for fodder for
animals. Clearly, integrating platyopuntias with other resources in the feeding system is beneficial.
Poor-quality roughage may be supplemented with
cladodes of Opuntia ficus-indica var. inermis. Indeed, the
intake of straw goes up significantly with the increase of
the amount of cladodes in the diet (Nefzaoui et al. 1993;
Ben Salem et al. 1996). Cladodes are also a good supplement to ammonia- or urea-treated straw, because they provide the necessary soluble carbohydrates for the efficient
use of nonprotein nitrogen in the rumen (Nefzaoui et al.
1993). For Barbarine sheep, voluntary intake of cladodes
(presented ad libitum) remains high (450 g dry weight
day-1) when 250 or 480 g day-1 of untreated, urea- or ammonia-treated straw is ingested (Nefzaoui et al. 1993). Diets
containing 64% cladodes and straw cause no digestive disturbance. Maintenance energy requirements for sheep are
met with the low level of straw intake, and for high level
the energy intake is 80% above the maintenance level.
Urea- or ammonia-treated straw is necessary to reduce the
nitrogen deficit. When the untreated straw plus cladode
diet is supplemented with Atriplex nummularia (about 300
g dry weight day-1) as the nitrogen source, even more nitrogen is retained in the sheep and the digestibility of organic matter and crude protein exceeds 70% (Nefzaoui
and Ben Salem 1996). For 4-month-old lambs, when
cladodes replace the more expensive barley grain, 10 to 15%
more organic matter is taken up and daily weight gain averages 15% higher (Table 12.5). Platyopuntias such as O.
ficus-indica can also be important for goats raised under
TA B L E 1 2 . 5
Intake, digestibility, nitrogen balance, and growth for lambs on straw-based diets supplemented with
conventional feeds (barley and soybean meal) and alternative feeds (Opuntia ficus-indica and Atriplex
nummularia)
Supplements
Soybean meal
Quantity
Organic matter intake
(g per [kg body weight]0.75)
Digestibility (%)
Organic matter
Protein
Fiber
Retained N (g day –1)
Daily weight gain (g day –1)
Atriplex
Barley
Opuntia
Barley
Opuntia
68
85
81
94
70
73
68
9.4
108
71
71
69
9.5
119
68
71
68
7.5
59
75
73
74
12.2
81
Data are for Tunisia and are from Ben Salem et al. (1998).
harsh conditions. Supplementing Alpine × local goats on
native rangeland with cladodes and A. nummularia (100 g
dry weight of each per day) substantially increases the daily
weight gains of these animals (by 25–60 g day-1; Ben Salem
et al. 2000), so fresh O. ficus-indica and other nonconventional feeds can have economic advantages for livestock
feeding.Tabe12l5n.eahrere:
Conclusions
Cladodes can be an important fodder in the WANA region, as clearly shown for sheep. Cladodes are not a balanced feed, but are a good, inexpensive energy and water
source. They are high in ash, Ca, soluble carbohydrates,
and vitamin A, but low in crude protein, fiber, P, and Na;
as cladodes age, they increase in dry matter and fiber but
decrease in crude protein. As for other good forage crops,
cladodes are highly digestible, with typical digestibilities of
60 to 70% for organic matter, 40 to 70% for crude protein, and 40 to 50% for crude fiber. When the level of
cladodes in the diet increases, the intake of fibrous feeds,
the rumen protozoa, and the rumen ammonia concentration increase while water intake and rumen cellulolytic activity decrease. Indeed, feeding cladodes can cause sheep
essentially to stop drinking water. Cladodes are highly
palatable—about 6 to 9 kg and 50 to 80 kg of dry weight
can be consumed daily by sheep and cattle, respectively.
Cladodes have a low gut fill value, because their intake
does not reduce fibrous feed intake. The nutritive value of
spiny and spineless cladodes are similar, and spines are
readily removed. Cladodes should be supplemented with
an appropriate and cost-effective nitrogen source (e.g.,
Atriplex spp.). Recommendations are (1) avoid decreasing
rumen cellulolytic activity by adding molasses, (2) limit the
amount of grain in the diet for the same reason, (3) feed
animals fibrous feeds (straw, hay) before cactus to minimize any laxative effect, and (4) use a special mineral supplement for adequate amounts of sulfur. In any case, the
high moisture content of cladodes alleviates the problem of
watering animals in dry regions.
Acknowledgment
An earlier version of this material focusing less on international aspects and more on specific sheep diets and with
different figures and tables appeared in the June 2000
CactusNet newsletter.
Literature Cited
Azocar, P. C., and H. C. Rojo. 1991. Uso de cladodios de
tuna (Opuntia ficus-indica) como suplemento forragero estival de cabras en lactancia, en reemplazo de
heno de alfalfa. Avances en Producción Animal 16: 173–
182.
Azocar, P. C., H. C. Rojo, J. J. Mira, and H. B. Manterola.
1996. Inclusión de nummularia (Atriplex nummularia
Lindl.) y cladodios de tuna (Opuntia ficus-indica) en la
dieta de cabras criollas, en reemplazo de heno de alfalfa. 1. Effecto en el consumo, peso vivo y producción de
leche. Avances en Producción Animal 21: 43–50.
Barbera, G., F. Carimi, and P. Inglese. 1992. Past and pres-
Forage, Fodder, and Animal Nutrition
207
ent role of the Indian-fig prickly-pear (Opuntia ficusindica (L.) Miller, Cactaceae) in the agriculture of
Sicily. Economic Botany 46: 10–20.
Barbera, G. 1995. History, economic and agro-ecological
importance. In Agro-ecology, Cultivation and Uses of
Cactus Pear (G. Barbera, P. Inglese, and E. PimientaBarrios, eds.). FAO Plant Production and Protection
paper 132, FAO, Rome. Pp. 1–12.
Castra, J., S. Pérez, and E. Riquelme. 1977. Evaluation of
thornless prickly pear silages as a feedstuff for ruminants. Proceedings Western Section, American Society of
Animal Science 28: 127–128.
Clovis de Andrade, J. 1990. As Palmas Forrageiras em
Alagoas. Maceio, Alagoas, Brazil.
Ben Salem, H. 1998. Effets de l’Acacia cyanophylla Lindl.
sur l’ingestion et la digestion des régimes destinés aux
ovins. Rôle des tanins et perspectives d’amélioration de
sa valeur alimentaire. Ph.D. Thesis, Université de
Dijon, Dijon, France.
Cordier, G. 1947. De la composition de quelques produits
fourragers tunisiens et de leur valeur pour l’alimentation du mouton. Annales du Service Botanique et
Agronomique de Tunisie 20: 22–108.
Ben Salem, H., A. Nefzaoui., H. Abdouli, and E. R.
Orskov. 1996a. Effect of increasing level of spineless
cactus (Opuntia ficus-indica var. inermis) on intake and
digestion by sheep given straw-based diets. Animal
Science 62: 293–299.
Curtis, R. J. 1979. Prickly pear farming in the Santa Clara
Valley, California. Economic Botany 31: 175–179.
Ben Salem, H., A. Nefzaoui, and L. Ben Salem. 1996b.
Nutritional characterization of some Mediterranean
fodder trees and shrubs harvested at different seasons.
Native and Exotic Fodder Shrubs in Arid and Semiarid
Zones. Regional Workshop, Hammamet, Tunisia. P.
23.
Cottier, H. 1934. Quelques aliments de disette, leur valeur
et leur emploi. La Tunisie Agricole 37: 127–141.
De Kock, G. C. 1965. Manejo y utilización del nopal sin
espinas. 9o Anales Congreso Internacional de Pasturas,
São Paulo, Brazil, 2: 1471–1474.
De Kock, G. C., and J. D. Aucamp. 1970. Spineless Cactus.
The Farmer’s Provision against Drought. Leaflet No. 37,
Agricultural Research Institute of the Karoo Region,
Pretoria.
Ben Salem, H., A. Nefzaoui, and L. Ben Salem. 1998.
Fodder shrubs as supplements to poor quality roughages fed to sheep. International Symposium “Livestock
Production and Climatic Uncertanty in the Mediterranean.” Agadir, Morocco. P. 21.
De Kock, G. C. 1980. Cultivation of drought tolerant fodder shrubs. In Browse in Africa: The Current State of
Knowledge (H. N. Le Houérou, ed.). International
Livestock Centre for Africa, Addis Ababa. Pp. 399–
410.
Ben Salem, H., A. Nefzaoui, and L. Ben Salem. 2000.
Supplementing range goats in central Tunisia with feed
blocks or a mixture of Opuntia ficus-indica var. inermis
and Atriplex nummularia. Effects on behavioural activities and growth. Proceedings of the 7th International
Conference on Goats. Tours, France. Pp. 988–989.
De Kock, G. C. 1983. Drought resistant fodder crops. In
Browse in Africa: The Current State of Knowledge (H. N.
Le Houérou, ed.). International Livestock Centre for
Africa, Addis-Ababa. Pp. 1–30.
Ben Salem, H., A. Nefzaoui, and L. Ben Salem. 2002.
Supplementation of Acacia cyanophylla Lindl. foliagebased diets with barley or shrubs from arid areas
(Opuntia ficus-indica var. inermis and Atriplex nummularia L.): Effects on growth and digestibility in
lambs. Animal Feed Science and Technology (in press).
Ben Thlija, A. 1987. Nutritional value of several Opuntia
species. Master’s Thesis, Oregon State University,
Corvallis.
Brutsh, O. M. 1984. Prickly pear (Opuntia ficus-indica)
cultivation in Southern Africa. Symposium of Agricultural Use of the Cactaceae. Prospects and Problems.
208
International Organization for Succulent Plant Study,
Frankfurt.
Nefzaoui and Ben Salem
Demeyer, D. I., and C. J. Van Nevel. 1979. Effect of defaunation on the metabolism of rumen micro-organisms. British Journal of Nutrition 42: 515–524.
El-Nouty, F. D., G. A. Hassan, T. H. Taher, M. A. Samak,
Z. Abo-Elezz, and M. H. Salem. 1988. Water requirements and metabolism in Egyptian Barki and
Rahmani sheep and Baladi goats during spring, summer and winter seasons. Journal of Agricultural Science,
Cambridge 111: 27–34.
Flores Valdez, C. A., and J. R. Aguirre Rivera. 1992.
El Nopal Como Forraje. Universidad Autónoma
Chapingo, Chapingo, Mexico.
Gonzalez, C. L. 1989. Potential of fertilization to improve
nutritive value of prickly pear cactus (Opuntia lind-
heimeri Engelm.). Journal of Arid Environments 16: 87–
94.
Gregory, R. A., and P. Felker. 1992. Crude protein and
phosphorus contents of eight contrasting Opuntia forage clones. Journal of Arid Environments 22: 323–331.
Griffiths, D. 1905. The Prickly Pear and Other Cacti as Food
for Livestock. United States Department of Agriculture
Bulletin 74, Washington, D.C.
Harvard-Duclos, B. 1969. Las Plantas Forrajeras Tropicales.
Blume, Barcelona.
Hoffman, G. O., and A. H. Walker. 1912. Prickly Pear—
Good or Bad? Texas Agricultural Experiment Station,
Bulletin 806, College Station, Texas.
Komisarczuk-Bony, S., and M. Durand. 1991. Effects of
minerals on microbial metabolism. In Rumen Microbial Metabolism and Ruminant Digestion ( J. P. Jouany,
ed.). Institut National de la Recherche Agronomique,
Paris. Pp. 179–198.
Le Houérou, H. N. 1979. Resources and potential of the
native flora for fodder and sown pastures production
in the arid and semi-arid zones of North Africa. In Arid
Land Plant Resources ( J. R. Goodin and D. K.
Worthington, eds.). ICASALS, Texas Tech University,
Lubbock. Pp. 384–401.
Le Houérou, H. N. 1991. Rangeland management in
Northern Africa and the Near East: Evolution, trends
and development outlook. Proceedings V International
Rangeland Congress, Montpellier, France. Pp. 543–552.
Le Houérou, H. N. 1992. The role of Opuntia cacti in the
agricultural development of Mediterranean arid zones.
Proceedings 2nd International Congress on Prickly Pear
and Cochineal, Santiago, Chile. Pp. 186–198.
Lozano, G. M. 1958. Contribución al estudio e industrialización del nopal (Opuntia spp.). Bachelor’s Thesis.
Universidad Autónoma de Mexico “Antonio Narro,”
Saltillo.
Metral, J. J. 1965. Les cactacées fourragères dans le Nord
Est du Brésil plus particulièrement dans l’état du
Ceara. Agronomie Tropicale 20: 248–261.
Monjauze, A., and H. N. Le Houérou. 1965. Le rôle des
Opuntia dans l’économie agricole Nord Africaine.
Bulletin École Nationale Supérieure d’Agriculture de
Tunis 8–9: 85–164.
Nefzaoui, A., and H. Ben Salem. 1996. Nutritive value of
diets based on spineless cactus (Opuntia ficus-indica
var. inermis) and Atriplex (Atriplex nummularia).
Native and Exotic Fodder Shrubs in Arid and SemiArid Zones, Regional Training Workshop. Hammamet,
Tunisia. P. 18.
Nefzaoui, A., and H. Ben Salem. 1998. Spineless cactus: A
strategic fodder for West Asia and North Africa arid
zones. International Symposium —Cactus Pear and
Nopalitos — Processing and Uses. Santiago, Chile. Pp.
58–76.
Nefzaoui, A., A. Chermiti, and H. Ben Salem. 1993.
Spineless cactus (Opuntia ficus indica var. inermis) as a
supplement for treated straw. 7th Meeting of the FAO
Sub-Network on Mediterranean Pastures and Fodder
Crops. Chania, Greece. Pp. 130–133.
Nefzaoui, A., H. Ben Salem, and L. Ben Salem. 1996. La
complémentation azotée du cactus inerme pour les
ovins. Effet de la source d’azote. Annales de Zootechuie
45: 20.
Nobel, P. S. 1988. Environmental Biology of Agaves and
Cacti. Cambridge University Press, New York.
Nobel, P. S. 1989. Productivity of desert succulents. Excelsa
14: 21–28.
Nobel, P. S. 1994. Remarkable Agaves and Cacti. Oxford
University Press, New York.
Nordblom, T., and F. Shomo. 1995. Food and Feed Prospects
to 2020 in the West Asia/North Africa Region. International Center for Agricultural Research in Dry Areas
(ICARDA), Aleppo, Syria.
Ørskov, E. R., F. D. Hovell, and F. Mould. 1980. The use
of nylon bag technique for the evaluation of feedstuffs.
Tropical Animal Production 5: 195–213.
Preston, T. R., and R. A. Leng. 1987. Matching Ruminant
Production Systems with Available Resources in the
Tropics and Sub-tropics. Penambul Books, Armidale,
Australia.
Rangnekar, D. V. 1988. Availability and intensive utilization of sugar cane by-products. In Non-Conventional
Feed Resources and Fibrous Agricultural Residues (C.
Devendra, ed.). International Development Research
Centre and Indian Council of Agricultural Research,
Hisar, India. Pp. 76–93.
Roussow, C. de W. 1961. The prickly pear native’s fodder
bank and silo. Farming in South Africa 37: 23–26.
Russell, C. E., and P. Felker. 1987. The prickly pears
(Opuntia spp. Cactaceae): A source of human and animal food in semiarid regions. Economic Botany 41:
433–445.
Forage, Fodder, and Animal Nutrition
209
Satter, L. D., and L. L. Slyter. 1974. Effect of ammonia
concentration on rumen microbial protein production
in vitro. British Journal of Nutrition 32: 199–208.
Silanikove, N. 1992. Effects of water scarcity and hot environments on appetite and digestion in ruminants: A
review. Livestock Production Science 30: 175–194.
210
Sustainable Livestock Production on Local Feed Resources.
Hanoi. Pp. 71–74.
Ushida, K., and J. P. Jouany. 1985. Effect of protozoa on
rumen protein degradation in sheep. Reproduction
Nutrition Development 25: 1075–1081.
Shoop, M. C., E. J. Alford, and H. F. Mayland. 1977.
Plains prickly pear is a good forage for cattle. Journal
of Range Management 30: 12–16.
Valdez, G. C., and V. C. Flores. 1967. Alimentación del
Ganado Lechero Estabulado en al Cd. Saltillo, Coahuila.
Escuela Nacional de Agricultural (Inedito), Chapingo,
Mexico.
Teles, F. F. F. 1978. Nutrient analysis of prickly pear
(Opuntia ficus-indica Linn.). Ph.D. Thesis, University
of Arizona, Tucson.
Viana, S. P. 1965. El uso del nopal sin espinas en la alimentación del ganado. 9o Anales Congreso Internacional
de Pasturas. São Paulo, Brazil. Pp. 1461–1464.
Terblanche, I. L., A. M. Mulder, and J. W. Rossouw. 1971.
The influence of moisture content on the dry matter
intake and digestibility of spineless cactus. Agroanimalia 3: 73–77.
Wehren, W. 1976. Eignung und Futterwert, besonders
Aminosäurenzusammensetzung von unkonventionellen Proteinquellen in semi-ariden Gebieten Tunesiens.
Ph.D. Thesis, University of Bonn, Bonn, Germany.
Theriez, M. 1965. Recherches sur la Digestibilité de Opuntia
ficus-indica var. inermis en Tunisie. Institut National de
la Recherche Agronomique, Tunis, Tunisia.
Wilson, A.D. 1970. Water economy and food intake of
sheep when watered intermittently. Australian Journal
of Agricultural Research 21: 273–281.
Tien, V. D., L. V. Ly, N. K. Duong, and B. Ogle. 1993.
The prickly pear cactus (Opuntia elator) as supplement
for sheep in the Phanrang semi-arid area of central region of Vietnam. National Seminar Workshop on
Woodward, T. E., W. F. Turner, and D. Griffith. 1915.
Prickly pear feed for dairy cows. Journal of Agricultural
Research 4: 405–449.
Nefzaoui and Ben Salem
CHAPTER
› 13 ‹
N O PA L I TO S , M U C I L AG E ,
FIBER, AND COCHINEAL
Carmen Sáenz-Hernández, Joel Corrales-García,
and Gildardo Aquino-Pérez
Introduction
Processing of Nopalitos
Harvest
Storage
Minimally Processed Nopalitos
Industrial Techniques
Nopalitos as Products
In Brine
Pickled
Sauces, Marmalades, Jams, Candies, and Juices
Mucilage
Dietary Fiber from Cladodes
Use of Cladodes in Medicine
Cochineal
Pre-Colonial and Colonial History
Decline and Resurgence
Importance and Uses
Carminic Acid
Biology of Cochineal
Origin and Diversity
Morphology and Life Cycle
Host
Environmental Factors and Natural Enemies
D. coccus versus Wild Cochineal
Marketing of Cochineal
Production and Prices
Supply and Demand
Conclusions and Future Prospects
Literature Cited
211
Introduction
A common use of cactus stems is nopalitos—tender young
cladodes—a traditional vegetable eaten fresh or cooked in
various dishes. Mexicans are the principal consumers.
Nopalitos are generally obtained from Opuntia ficus-indica,
O. robusta, or Nopalea spp. The number of spines and the
color are quality factors evaluated by consumers. Several
new consumable products are being developed from the
stems (e.g., marmalades, blends for breakfasts, and pickles),
and the cladodes have medical and cosmetic uses.
Another important use of Opuntia stems is the production of carminic acid, a natural colorant developed by
the precolonial indigenous people of Mexico. Carminic
acid is produced by an insect known as cochineal, or cochinilla del nopal, a parasite that infests several species of
cacti belonging to two closely related genera, Opuntia and
Nopalea. Identified by the scientific name, Dactylopius, the
genus has been recognized worldwide since the 16th century as the source of a valuable red pigment, whose main
component is carminic acid. Native to the Americas,
cochineal was known in colonial times as nocheztli or grana
in New Spain, and as macno or magno in the Andean region of South America.
The chemical composition of cladodes determines
their use as a raw material in the food industry. Cactus
stems, as other vegetables, have low protein and fat contents (Table 13.1). The crude fiber is higher than in most
other vegetables and is an important consideration for
human health. The nitrogen-free extract content is high
and includes soluble dietary fiber, insoluble dietary fiber,
and some sugars. The ash depends on the soil composition,
but the main components are calcium and potassium;
sodium and phosphorus are present in lesser amounts.
Calcium oxalate crystals, which are insoluble in water, increase with age and can constitute 85% of the ash of old
cladodes (Pimienta Barrios 1990; Granados and Castañeda
1991; Sáenz et al. 1997). The crude fiber also increases with
cladode age (Table 13.1). This is significant, because young
nopalitos can be used as a fresh vegetable in salads, whereas old nopalitos, whose high fiber content makes them
difficult to chew, are useful for other purposes. Cladodes
have high concentrations of phenylalanine, leucine, and vitamins (retinol and ascorbic acid; Zambrano et al. 1998
Processing of Nopalitos
Nopalitos have formed part of the diet of Mexican people
since pre-Hispanic times; nowadays, they are also a specialty
vegetable in the United States. Production in Mexico is
about 600,000 tons fresh weight per year; under intensive
212
Sáenz-Hernández, Corrales-García, and Aquino-Pérez
management involving close planting in irrigated and fertilized beds that are often covered with plastic, productivity can be 250 tons ha-1 year-1 (Flores 1997; Nobel 1998). In
Mexico, people prefer to buy thin and turgid cladodes with
a fresh appearance and a brilliant green color. They are
cooked at home as an ingredient in various recipes for stews,
dishes and desserts. Outside Mexico, people of Mexican origin can purchase processed nopalitos. Young cladodes are in
a rapid growth phase and have high metabolic activity and
high transpiration rates (Cantwell 1991). However, once
they are harvested as nopalitos, they have moderate respiration rates (25 microliter CO2 g-1 hour-1 at 20°C) and a low
ethylene production (0.2 nanoliter g-1 hour-1 at 20°C;
Cantwell et al. 1992). Nopalitos are highly perishable after
harvest; the deterioration processes lead to wilting, browning, and microbial contamination by Alternaria sp.,
Penicillium sp., and other fungi (Ramayo-Ramirez et al.
1978a), especially when they have been despined and diced.
Harvest
Cladodes for nopalitos are harvested by hand, gripping the
bottom of a pad and twisting more than 90° until it snaps
off the mother plant. The lower tissues can be torn if this
action is not carried out with care, so detached cactus
stems and the mother plants can easily be infected by microorganisms. For this reason, cutting a pad at its base with
a knife is better than simply twisting it off. In any case, harvested nopalitos must be protected from the sun to lessen
metabolism, transpiration, and infection (Corrales 1992a).
After harvest, intact nopalitos (with spines) are directly
taken to local markets; for distant markets, nopalitos are
packed in a shady area.
The form in which nopalitos are transported depends
on where the sale takes place and on the distance to the
market. The following modalities occur: (1) transportation
of intact or despined nopalitos over short distances in vans
for sale by bulk in local markets; (2) intact or despined
nopalitos, packed in baskets called colotes containing approximately 200 pads; (3) 500 to 550 intact nopalitos
packed in sacks for the large markets of Mexico City; (4)
intact or despined nopalitos packed in cardboard boxes or
wooden crates of 10 to 15 kg capacity, when the market is
in California or in Mexico near the U.S. border; and (5) intact nopalitos packed in a cylindrical packing unit (paca),
which is the main mode used for the large markets of
Mexico City and other cities in central Mexico (Nobel
1998). The latter packing unit is 1.6 to 1.7 m in height and
0.7 to 0.8 m in diameter, containing 2,500 to 3,000 pads
(Corrales 1992a). For a paca, nopalitos are placed horizontally on cloth as a layer inside a metal mold; following its
TA B L E 1 3 . 1
Chemical composition of cladodes (% of dry matter)
Age
(years)
1
2
3
4
Protein
(%)
Fat
(%)
Crude fiber
(%)
Nitrogen-free
extract
(%)
Ash
(%)
5.4
4.2
3.7
2.5
1.29
1.40
1.33
1.67
12.0
14.5
17.0
17.5
63
67
64
64
18.2
13.2
14.2
14.4
Reference: López et al. (1977), cited by Pimienta (1990).
circular form, another nopalito layer is placed upon the
first one, and so forth (Corrales-García 1997). Pacas have
proved practical, especially when periods of commercialization are short (1–3 days). If the period is longer, considerable heat is generated in the center of the packing
units by respiration of the nopalitos (Cantwell 1991), reducing the quality of product.
Storage
Refrigeration atmospheres with reduced O2 and/or elevated CO2 concentrations extend the storage life of many
fruits and vegetables by reducing respiration rates.
However, postharvest deterioration can result from many
factors besides high respiration rates, including the biochemical changes associated with respiratory metabolism,
ethylene production and action, compositional changes,
physiological disorders, and pathological breakdown
(Kader 1986). Furthermore, under certain conditions, atmospheric changes shift cladodes from aerobic to anaerobic respiration, leading to fermentation and the accumulation of ethanol and acetaldehyde (Chang et al. 1982) and
causing unpleasant flavors and odors.
Storing at low temperatures extends the shelf life of
nopalitos and maintains their vitamin content. This is especially true under modified atmospheres, which also implies low O2 availability for oxidation and browning, low
degradation of vitamins, and, in general, low enzymatic activity. Other factors, such as harvest technique, storage duration and relative humidity, and packing technique also
affect the shelf life of nopalitos (Cantwell 1995). Ramayo et
al. (1978b) found that 21% of pads packed in wooden crates
and stored at 10°C (at 80–85% relative humidity) showed
decay at the cut surface at 10 days; however, if carefully harvested, the shelf life can be extended to 21 days without
decay development under the same storage conditions.
Nopalitos are susceptible to chilling injury when exposed to nonfreezing temperatures below 10°C. This phys-
iological disturbance is typical of some tropical and subtropical fruits and vegetables (Saltveit and Morris 1990;
Wang 1990) as well as nopalitos (Ramayo-Ramírez et al.
1978a; Cantwell 1991) and leads to surface discoloration and
softening, which, in turn, usually promotes microbial infections. The susceptibility to chilling injury and its consequences vary with species and stem age, the harvest method,
and the method of packing as well as with the atmosphere,
temperature, relative humidity, and duration of cold storage. Nopalitos of Opuntia spp. packed in vented polyethylene bags (leading to a modified atmosphere inside the
bag) may show signs of chilling injury at 21 days of storage
at 5°C, whereas cactus stems packed in wooden crates and
also stored at 5°C show chilling injury at 15 days (RodríguezFélix and Villegas-Ochoa 1998). Stems of Nopalea cochenillifera are more susceptible to chilling injury during storage
at 4°C than are those of Opuntia spp. Outside of bags, they
develop symptoms of chilling injury at 7 days, but if stored
in plastic bags, an additional 4 days of storage is gained before symptoms appear (Nerd et al. 1997).
Minimally Processed Nopalitos
In general, “minimally processed” horticultural products
are prepared and handled to maintain their freshness
while providing convenience to the consumer. Producing
minimally processed products involves cleaning, washing,
trimming, coring, slicing, and shredding (Brecht 1995;
Schlimme 1995). Other terms used to refer to minimally
processed products are “lightly processed,” “partially
processed,” “fresh-processed,” and “preprepared” (Cantwell
1992). According to Avena (1996), minimum processing includes the operations generally used for canned, frozen, or
dehydrated food products, but without scalding for inactivation of enzymes. Most of these products are sold as
ready-to-eat foods, which is a major advantage. “Pre-cut,”
“minimally processed,” or “fresh-cut” can describe a special
modality for postharvest handling of fresh nopalitos.
Nopalitos, Mucilage, Fiber, and Cochineal
213
RECEPTION
DICING OR
CUTTING 3
WASHING
WEIGHING
NOPALITOS
FOR
PROCESSING
SAMPLING 1
AND
INSPECTION
WEIGHING
1
2
3
4
SCALDING
OR
COOKING 4
Spread out on tables (manually)
Optional; it is preferable to receive raw material without spines
Optional; this may be done after desalting, manually or mechanically
Optional; addition of spices
DESPINING 2
Figure 13.1. Flow diagram for conditioning raw nopalitos for later processes. Adapted from CorralesGarcía (1998).
Cleaning and despining of nopalitos—the latter an obligatory postharvest practice for nopalitos—is usually done at
the destination market just before the retail sale. This practice is generally carried out using a knife by people with
highly developed skill. Nowadays, diced pads, whose
spines have been removed, are packed in plastic bags and
sold in Mexico and the southwestern United States.
Nopalitos whose spines have been cut off have higher
metabolic activity and transpiration rates than do intact
nopalitos. Also, mucilage leaking, wilting, and oxidation
occur quickly, and their shelf life is short. For these reasons,
despined and diced nopalitos are more perishable than is
the intact product. In general, the main problems that
limit shelf life of minimally processed nopalitos are brown
discoloration at the cut surfaces (caused by polyphenol oxidases), mucilage leakage (undesirable mucilage accumulation inside the bag, which increases with dicing), unpleasant flavor caused by accumulation of anaerobic
metabolites, and a surface yellowing, which leads to a color
change from brilliant green to brownish green and gives
them a cooked appearance. Vacuum packing of diced pads
does not increase the shelf life at refrigerated storage temperatures (Rodríguez-Félix and Soto-Váldez 1992). Also,
the type of packaging, the storage temperature, and the
local atmosphere affect storage of minimally processed
nopalitos. For instance, 4°C is better than 10°C for storage
up to 15 days, and polypropylene (25 µm thick) is better
than polyethylene (35 µm thick).
214
Sáenz-Hernández, Corrales-García, and Aquino-Pérez
Industrial Techniques
Many alternatives for processing cladodes, nopalitos, and
tuna fruits have been described (Colin 1976; Corrales
1992b; Sáenz 1995). Industrially processed nopalitos can last
longer than fresh ones, allowing sales in distant markets.
Also, the supply can be extended to other months, and
prices and quality can be controlled. The variety of products obtained by industrialization adds value to the product and makes diversification of markets possible.
Industrialization also generates rural employment, which
contributes income that benefits the producer communities
(Corrales 1992b). Today in Mexico some companies process
nopalitos mainly for export, because domestic consumers
prefer to buy fresh nopalitos and then cook them at home.
The first steps in industrial processing of nopalitos are
reception and conditioning of the raw material, which
should be of the highest quality and be despined by the
grower (Fig. 13.1). Conditioning consists of scalding and
washing. Scalding deactivates enzymes, destroys microorganisms, softens the tissues, and partially eliminates the
mucilage. The main variables are temperature and duration
of scalding, as well as additives that improve the product.
Nopalitos of the wild nopal ‘Tapón’ (Opuntia robusta) lend
themselves to higher temperature and longer cooking time
than do cultivated nopalitos, such as the variety ‘Milpa
Alta’ (Opuntia ficus-indica). The final product is washed
with cold clean water, which fixes the characteristic green
Figure 13.2. Various brands and preparations of processed nopalitos available in Mexico as collected by the Programa Nopal (Cactus Pear
Program) of Centro de Investigaciones Económicas, Sociales y Tecnológicas de la Agroindustria y la Agricultura Mundial (CIESTAAM),
Universidad Autónoma Chapingo, México.
color of nopalitos and eliminates adhering pectins and mucilage (Corrales-García 1998). The resulting nopalitos can
be used for various products, e.g., nopalitos in brine, pickled nopalitos, and marmalades.Fgiure1n3.eahrere:
Nopalitos as Products
In Brine
Nopalitos can be preserved in a saline solution (maximum
2% NaCl) and then canned, placed in plastic bags, or
packed in glass or plastic jars to be used later to prepare
various Mexican foods. More than 20 brands are currently available in Mexico (Fig. 13.2), many with their own
preparation process. The conditioned nopalitos (Fig. 13.1)
are commonly salted in large tanks containing 12% NaCl
brine, using approximately 1.7 liters of brine for each kilogram of nopalitos (Fig 13.3). Nopalitos must remain in
these tanks for at least 10 days and can be kept there for
months. The high concentration of salt extracts water from
the nopalitos, so salt must be added to maintain the brine.
The tanks should be stirred daily with a wooden paddle.
Also, the tanks should be covered to avoid contamination
and discoloration of the product by light.Fgiure132.neahrereF:giure13n.eahrere:
When salting is finished, the product is taken to the
process room, where it is desalted by washings (Fig. 13.3).
Later, it is sorted, diced, and canned, or put into plastic
bags or jars with a few spices and covered with 2% brine.
The bags are sealed; the cans or jars are evacuated, covered,
and sterilized in an autoclave or water-bath, then left to air
dry before labeling. Bags, cans, or jars are packed in cardboard boxes and stored for the quarantine period; eventually they are shipped to the market. The product can also
be sold in bulk without desalting (Fig. 13.3).
Compared with intact nopalitos (with spines), the final
yield of nopalitos in brine ready for sale is about 57%, depending on the process and its control. The following procedures can improve success: (1) implement a well-defined
program and rigorous quality control beginning with the reception of the raw material — e.g., remove pads that are
bruised or flawed; (2) implement a quarantine program that
maintains the product’s quality for as long as possible; (3)
avoid direct contact of the brine with a reactive metal (such
Nopalitos, Mucilage, Fiber, and Cochineal
215
CONDITIONING
MARKET BY BULK
OR IN BAGS 3
TANKS 1
WASHING (DESALTING) 4
STIRRING AND COVERING 2
SELECTION
CUTTING 5
ADDITION OF SPICES AND
PRESERVATIVES AND
COVERING WITH LIQUID 7
PUTTING INTO
JARS OR BAGS 6
LABELING
PACKING 9
COVERING AND SEALING 8
STERILIZATION AND
AIR DRYING OF JARS
STORAGE 10
MARKET
1
12% brine
Daily stirring with wooden paddle
3 In wooden or plastic containers
4 During approximately 6 hours, until reaching 1-2% salt
5 Optional: manual or mechanical
6 In plastic bags or glass or plastic jars
7 2% brine
8 Brine must cover nopalitos completely (to avoid air reaching the pads)
9 In cardboard boxes
10 Quarantine
2
Figure 13.3. Flow diagram for processing nopalitos in brine. Adapted from Corrales-García (1998).
as unpainted iron); (4) maintain the brine at a minimum
NaCl concentration of 10% that is verified constantly with
a special salt meter, and stir daily to help ensure uniform
salinity; (5) completely cover the nopalitos with brine (weigh
them down with a plastic or wooden screen); and (6) because light and extraneous material (e.g., dust, dirt, litter,
water, insects) are detrimental, cover the tanks.
Pickled
Pickled nopalitos consist of scalded nopalitos preserved in
vinegar (maximum 2% of acetic acid) with spices and vegetable seasonings (García 1993). More than 25 companies
in Mexico currently pickle nopalitos, and many have their
216
Sáenz-Hernández, Corrales-García, and Aquino-Pérez
own preparation processes (Fig. 13.2). The conditioned
nopalitos (Fig. 13.1) are cut or diced (manually or mechanically; Fig. 13.4). Pickling is done with vinegar (1.9–
2.0% acetic acid), spices, aromatic herbs, and olive oil. The
vinegar is heated to boiling, and then the spices are added,
either directly or in a cloth bag. The mixture is boiled for
5 minutes to allow the vinegar to absorb the aromas.
Separately, onion slices, garlic cloves, laurel leaves, and carrot discs are lightly fried in vegetable oil. Then the nopalitos, vinegar, and sautéed vegetables are mixed. This mixture is canned, or put into plastic bags or jars. The bags are
sealed, and the cans and jars are evacuated and covered;
they are then sterilized in an autoclave or in a water-bath.
DICING OR CUTTING 6
CONDITIONING
PREPARATION OF
SAUTEED VEGETABLES 3
PREPARATION OF
BRINE 2
MIXING WITH
HEATING
SEALING
AND
LABELING
PUTTING INTO JARS
OR BAGS
COOLING AND AIR
DRYING OF JARS
OR BAGS
PACKING
IN
CARDBOARD BOXES
STERILIZATION
STORAGE 4
MARKET
1
Manually or mechanically
Vinegar (1.9-2.0% acetic acid)
3 Onion slices, garlic, laurel leaves, and carrot discs
4 Quarantine
2
Figure 13.4. Flow diagram of production process for pickled nopalitos. Adapted from Corrales-García
(1998).
Finally, they are left to air dry before labeling. Bags, cans,
or jars are packed in cardboard boxes, stored for the quarantine period, and then shipped to the market.Fg4nui3.1heare:
Sauces, Marmalades, Jams, Candies, and Juices
Nopalito sauces are prepared using milled nopalitos, with
addition of various chilis, tomato, onions, vinegar, and
spices (in different proportions), and, often, a preservative.
More than 15 brands of nopalito sauce occur, some with
white wine or lemon concentrate. The sauces can have
whole pieces of nopalito or be totally milled, depending on
the market preference. In Mexico, nopalito sauces are generally prepared daily using fresh ingredients, rather than as
canned sauces for the market. In addition to sauces, recently in Mexico nopalitos are incorporated into sausages
using soybean flour, and nopalitos are prepared with tuna
fish, beans, jalapeño chilis, and mushrooms. These products are prepared by large, established companies, following principles of modern food technology.
Another product from nopalitos is marmalade. This
product is prepared using milled nopalitos cooked with
various concentrations of sugar, pectin, and preservatives.
The conditioned (scalded) nopalitos (Fig. 13.1) are chopped
(manually or mechanically) and cooked for a second time.
Tirado (1986) made a jam with cladodes, adding orange
juice, peel, and sugar in the ratio of 1:1.5:0.8:0.8. The jam
had no microbial growth after 40 days of storage. This
product is similar to other jams in the Mexican market
(e.g., fig and orange) with respect to aroma, color, taste,
texture, and appearance.
Badillo (1987) made a jam using cladodes, sugar, and
citric acid, obtaining a product with good sensory quality
and microbiological stability. Sáenz et al. (1995a) made a
marmalade from cladodes after treating with 2% Ca(OH)2
to lower the mucilage content (which causes texture and
acceptability problems). Lemon juice and lemon peel
were included; the first lowered the pH and the second, together with pectin, aided the gelling of the product
(67°Brix, 0.97% acidity, and good acceptability). About six
companies in Mexico and the United States presently
Nopalitos, Mucilage, Fiber, and Cochineal
217
manufacture marmalades. Mucilage obtained by milling
and filtrating nopalitos can lead to better consistency in
marmalades of other fruits (e.g., blueberry, raspberry,
blackberry, strawberry, peach, apple, pear, pineapple, and
plum), and therefore has potential to expand the world
market for nopalito products.
Candies made with nopalitos are processed with sugar
and often various other ingredients. The main confectionary products include crystallized nopalitos, nopalitos
in syrup, nopalito candies covered with chocolate, marshmallows with nopalito mucilage (gomitas), candies of nuts
cooked with honey and platyopuntia mucilage. Villareal
(1996) studied the manufacture of crystallized cladodes,
which are similar to crystallized melon peel. Sucrose or
sugar-cane syrup can be used, and the candies are especially
enjoyed by children. The cladodes are cut into pieces that
are 1.8 × 4.0 cm, treated with Ca(OH)2 (to remove the mucilage), washed, osmotically dehydrated with a high concentration sucrose solution, and further dehydrated in a
forced-draft oven at 60°C. The final product has an intermediate moisture content and can be covered with sweet
or bitter chocolate to be more attractive to consumers.
Rodríguez (1999) developed a nopal juice using young
pads by scalding, milling, and filtering, then adding citric
acid and aspartate. The juice was put in bottles, pasteurized, and vacuum sealed. The product has a pleasant sweet
taste, brilliant green color, only 10 calories per bottle, and
10% nopal pulp. A mixed juice of nopal and guava is being
marketed nationally and internationally by a Mexican
company. Despined and diced nopalitos are milled in a
blender with water, the thick juice is filtered to separate
solids, the filtered juice is mixed with guava juice, and
finally the mixture is pasteurized and bottled. The world
market for fruit and vegetable juices has expanded, so
nopal juices, mainly in mixtures with other fruits, offer
great possibilities for development.
Mucilage
The complex polysaccharide mucilage is an important
component of platyopuntias. Mucilage has great potential
as part of dietary fiber and also imbibes large amounts of
water, forming viscous or gelatinous colloids (Amin et al.
1970; McGarvie and Parolis 1979a,b, 1981; Paulsen and
Lund 1979; Trachtenberg and Mayer 1981; Sáenz et al.
1992). Mucilage is composed of varying proportions of Larabinose, D-galactose, L-rhamnose, D-xylose, and galacturonic acid, the latter representing 18 to 25% of the
residues, depending on whether the mucilage comes from
fruit or cladodes (Sáenz 1995). The primary molecular
structure is a linear chain containing galacturonic acid,
218
Sáenz-Hernández, Corrales-García, and Aquino-Pérez
rhamnose and galactose, to which xylose and arabinose
residues are attached in peripheral positions.
Mucilage can be used as a thickening agent in foods
and pharmaceutical products. Sáenz et al. (1992) showed
that increasing the pH from 2.6 to 6.6 increased the viscosity of water dispersions of mucilage from 37 to 58 centipoise (37–58 mPa s). Cárdenas and Goicoolea (1997) and
Cárdenas et al. (1997) studied the rheological properties of
mucilage of different concentrations (0.4 to 6%) with
NaCl (0.1 M) and report that the non-Newtonian shearing
behavior is similar to that of okra mucilage solutions. In
particular, with increasing mucilage concentration a strong
tendency occurs for aggregation. Nobel et al. (1992) report
that the mucilage content of cacti varies with species and
is influenced by irrigation and temperature. For instance,
for four sympatric cacti from the Sonoran Desert, mucilage
is absent from Ferocactus acanthodes, is 19% of the dry
weight for Opuntia basilaris, 26% for O. acanthocarpa, and
35% for Echinocereus engelmannii; L-arabinose varied from
17 to 51% of the sugar monomers. For Opuntia ficus-indica
mucilage in the cladodes increases 24% as the day/night air
temperatures during growth are reduced from 30/20°C to
10 /0°C (Goldstein and Nobel 1991). For the widely occurring, cold-hardy Opuntia humifusa, mucilage in the
stems approximately doubles when plants growing at
day/night air temperatures of 25/15°C are transferred to
5 /–5°C for 7 weeks (Loik and Nobel 1991).
With regard to special applications, farmers in Chile
and some other countries use cactus mucilage to clarify
drinking water. As for other water-soluble polymers, mucilage flocculates sediment particles and precipitates them
out of solution (B. Crabb, personal communication).
Another traditional use by the farmers in Chile is to take
advantage of the adhesive properties of mucilage to improve external paint; chopped cladodes are blended with
lime (mostly Ca[OH]2) and applied to the external walls
of houses. Cladode mucilage has also been used for a long
time as a glue in combination with lime plaster in Mexico.
Mucilage helps the lime to set more quickly and improves
the water repellency. This plaster is traditionally used over
both earthen (adobe) and brick walls and also as a breathing water-barrier in stucco. Gardiner et al. (1999) found
that a cladode extract improves water infiltration in soils,
similar to the effects of polyacrylamides. Cactus mucilage
also has culinary uses, such as a fat replacer and a flavor
binder ( J. McCarthy, cited in Cárdenas et al. 1997).
Dietary Fiber from Cladodes
The market in developed countries is increasing for healthful foods with low calories, low cholesterol, low fat, and a
high fiber content. Studies showing the relation among
fiber consumption and control of cholesterol as well as the
prevention or treatment of some illnesses, such as diabetes,
obesity, gastrointestinal disorders associated with a lack of
dietary fiber intake, and even colon cancer (Sloan 1994;
Grijspaardt-Vink 1996; Hollingsworth 1996), have helped
to promote this market. Dietary fiber is composed of several chemical components that are resistant to digestive enzymes, e.g., cellulose, hemicellulose, pectin, lignin, and
gums (Spiller 1992; Periago et al. 1993). The fiber content
of a food varies with the plant species and the stage of maturity, but seeds, berries, fruit skins, and the bran layers of
cereal grains generally contain a large amount of fiber.
Based on water solubility, soluble dietary fiber is contributed by mucilage, gums, pectin, and some hemicelluloses, and insoluble dietary fiber by cellulose, lignin, and
most hemicelluloses (Periago et al. 1993). Nopal cladodes
(i.e., nopalitos) are a good source of dietary fiber.
Sepulveda et al. (1995) obtained a natural concentrate
of nopal fiber (“nopal flour”) using 2- to 3-year-old cladodes obtained from pruning. Sáenz et al. (1997) and Sáenz
(1998) studied the dietary fiber content and some physical
and chemical characteristics of this concentrate, as well as
the effect of concentration (2.5, 5.0, and 7.0%), temperature, and pH on the viscosity. Viscosity of nopal flour suspensions is an important parameter when the nopal flour
is mixed with other food ingredients; the pH also affects
such suitability (Lecaros 1997). Nopal flour consists of 43%
total dietary fiber, of which 28% is insoluble and 15% is soluble. Rosado and Díaz (1995) reported a dietary fiber content in dehydrated nopal of 50%, indicating that the type
of Opuntia, the climatic conditions, irrigation, and/or the
age of the cladodes can influence the dietary fiber. The
water-holding capacity in the former case is 5.6 g per g dry
mass, and in the latter case is 11.1 g g-1 dry mass for cladodes and 7.1 g g-1 for a nopal isolate. The water content indicates the physiological status of the fiber, as water absorption increases the bolus and produces a satiation
effect. The water absorption ability depends mainly on
particle size and can be modified by controlling the milling
process: the smaller the size of the particles, the greater the
water retention, as for wheat bran flour.
Nopal flour is being tested for various foods, such as
vegetable soup and a gelled dessert (Albornoz 1998; Vallejos
1999). The percentage of added flour is limited: greater
than 20% affects the texture of the product. Sáenz et al.
(1995b) and Fontanot (1999) tested different replacement
proportions of wheat flour by nopal flour in biscuits; more
than 15% replacement affects the texture and sensory
characteristics of the biscuits but increases the dietary fiber
content. Recently, a dehydrated pelletlike product made
from dehydrated cladodes has appeared in the Mexican
market. This product, which is a blend of wheat fiber,
nopal fiber, salt, and the sweetener aspartame, is similar to
a common breakfast cereal and is recommended to help
control obesity.
Use of Cladodes in Medicine
According to popular medicine, mainly in Mexico, many
diseases can be fought and cured with the cladodes, fruit,
or other parts of cacti, such as the flowers (Hegwood 1990;
Pimienta Barrios 1990; Barbera 1991; Mulas 1993). Nevertheless, only a few applications have a strong scientific
basis, such as their effect on diabetes mellitus, blood glucose levels, hyperlipidemy (excess of lipids in the blood),
and obesity (Gulías and Robles 1989).
Frati-Munari et al. (1990) studied the hypoglycemic
effect of cladodes of Opuntia ficus-indica, concluding that
glycemia decreased in all patients tested following ingestion
and reached statistically significant lower levels after 3
hours; Ibañez-Camacho et al. (1983) confirmed this hypoglycemic action. Ramírez and Aguilar (1995) in a metaanalysis conclude that Opuntia has a strong glucose reduction effect. Trejo et al. (1995) evaluated the hypoglycemic
activity of a purified extract from platyopuntias on STZ-induced diabetic rats; although the mechanism of action is
unknown, the magnitude of the glucose control by the
small amount of Opuntia extract required (1 mg per kg
body weight per day) precludes a predominant role for dietary fiber. Hernández et al. (1997, 1998) used Wistar rats to
compare the effect on weight loss of the consumption of
nopal fiber and other vegetable fibers, such as cellulose and
corn peel fiber. The nopal fiber produces more feces than
the other fibers, although all rats lost weight during the
study.
Frati-Munari et al. (1992) evaluated the role of commercial capsules (Fig. 13.5) containing dried and ground
cladodes in the management of diabetes mellitus. Thirty
capsules, each containing 335 mg of dried cladodes, were
given to diabetic subjects, and serum glucose was measured
throughout 3 hours; the control was performed with 30
placebo capsules. The dried cladodes did not show a hypoglycemic effect and did not influence the glucose tolerance
test. In diabetic patients, serum glucose, cholesterol, and
triglycerides levels did not change with ingestion of Opuntia
cladodes. In healthy individuals, glycemia did not change
with cladode ingestion, whereas cholesterol and triacylglycerides decreased. Fernández et al. (1990) found effects of
platyopuntia cladodes on low-density lipoproteins, suggesting that an extract may decrease cholesterol levels.:herenrae5.31Fgueri
Nopalitos, Mucilage, Fiber, and Cochineal
219
A
B
Figure 13.5. Capsules and tablets prepared from dried Opuntia cladodes in Mexico: (A) packaging and (B) contents.
Figure 13.6. Various brands and preparations of Opuntia cladode extracts used cosmetically in Mexico, as collected by CIESTAAM (see Fig. 13.2.).
More than 30 brands of powders, capsules, and tablets
made of dried platyopuntia cladodes are produced in
Mexico as nutritious complements (Fig. 13.5). Powder is
prepared from cladodes (1.5–2.5 years old) that are washed
(chlorinated water), despined, cut, and then dehydrated (at
35–40°C), preferably with forced air. They are then milled
and screened, until a fine powder is obtained. This powder
is sold in bulk or encapsulated, or it is added with an agglutinant and then compressed to obtain tablets (FratiMunari et al. 1992; Sepúlveda et al. 1995). Such products are
marketed by promoting their alleged medicinal effects, and
consumers consume them as medications. Indeed, cladodederived products are sold for the control of diabetes, cholesterol, gastric and intestinal afflictions, and obesity. This
utilization is due to three main factors: (1) the existence of
customs, traditions, and pre-Hispanic knowledge of medicine and the traditional herbalists of Mesoamerica (cladodes can cure renal illnesses and erysipelas, induce childbirth, alleviate pain, and heal wounds); (2) various medical
studies indicating that the consumption (ingestion) of raw,
boiled, roasted, or stewed cladodes decreases glucose and
cholesterol in blood in healthy and certain diabetic people,
but not in insulin-dependent animals or humans (Ibañez-
Camacho and Roman 1979; Ibañez et al. 1983; Fernández et
al. 1990; Meckes and Roman-Ramos 1986; Frati-Munari et
al. 1989; Trejo et al. 1991); and (3) a modern trend toward
“natural” product consumption.
The production of cladode products as over-the-counter medicinal products is growing fast. Frequently, these
products have scientifically unfounded claims as to their
healing properties. Currently, 21 companies in Mexico
produce capsules, 15 produce tablets, and five produce
powders (Fig. 13.5). Some of these companies export their
products to the United States as nutritious complements.
The hypoglycemic effects that many of these products purport to have is unproved (Frati-Munari et al. 1992). At least
30 capsules may need to be consumed per day, which is not
comfortable for the patients.
Cosmetics (Fig. 13.6) incorporating cladodes are used
for hygienic purposes to beautify the skin and the hair, in
accordance with the herbalists of Mesoamerica and popular as well as traditional knowledge. In Mexico more than
20 companies manufacture more than 40 different cosmetic products with platyopuntias, adding portions of
juice of the cladodes in their formulations. These products,
being of “natural” origin, are increasingly accepted in
Nopalitos, Mucilage, Fiber, and Cochineal
221
Mexico and the United States. However, the cosmetics industry does not require large quantities of cladodes, because they are only a small ingredient in the formulations
of these products. The principal cosmetic products (Fig.
13.6) are: (1) shampoos, conditioners, and gels for the hair;
(2) soaps; (3) creams as well as facial masks to reconstitute,
moisten, clean, or strengthen the skin; and (4) astringent
or absorbent lotions (to reduce epidermal fat).Fgiure136.neahrere:
Cochineal
Two types of cochineal, the dye-producing cactus parasite,
are recognized. They are classified according to the quality and concentration of their pigments, as well as their biological and morphological characteristics: (1) fine
cochineal (Dactylopius coccus Costa), and (2) wild cottony
cochineal, comprising a group of eight species. Though a
noxious pest in nopal fruit orchards, wild cochineal is
beneficial as a biological control of weedy nopal infestations throughout the world. Both D. coccus and cottony
cochineal are easily propagated in various microclimates of
North and South America. Although Mexico’s central,
southern, and southeastern highlands harbor a great diversity of both Opuntia and Dactylopius and the Mesoamerican region has the oldest records of systematic exploitation of cochineal, Mexico is presently not a major
commercial producer of cochineal. The Andean region of
South America, noteworthy for having the oldest evidence
of cochineal use, currently accounts for 95% of world production. Use of the dye has spread to all nations except several Middle Eastern and Asian countries. Cochineal depends on platyopuntias for its propagation and survival,
making its role as host one of the most important uses of
Opuntia. Therefore, until technological development obviates the need of a host in the cultivation of cochineal,
platyopuntias will be a determining factor in the production of the dye insect.
Pre-Colonial and Colonial History
Archaeological evidence for the use of cochineal exists in
prehistoric textiles recovered from the Nazca (Classic) and
Chimu (Postclassic) cultures of Peru. Saltzman (1992) reports incipient use of Dactylopius around the time of
Christ and increased utilization in woolen textiles from the
late Classic period (7th century). By the 10th century,
cochineal was in common use. The first historical reports
of cochineal from Peru date from 1533 (Donkin 1977).
Evidence of pre-Hispanic cochineal use in Mexico is surprisingly scarce; only a few fragments of pre-Conquest
cochineal-dyed fabrics have been found (Donkin 1977).
Nevertheless, cochineal was a tribute item in Aztec and
222
Sáenz-Hernández, Corrales-García, and Aquino-Pérez
Inca empires, in the form of richly colored woolen and cotton mantles (Donkin 1977) and in dye taxed by the Aztec
governors. Two basic forms were used: (1) dried cochineal,
and (2) cakes or tablets (panes, pastillas), known as nocheztlaxcalli in Nahuatl, produced by the Indians from the
dried, milled insect, leaves of the tezhoatl tree, and alum
(Dahlgren 1963; Donkin 1977). In contrast to wool, cotton
is difficult to dye with cochineal alone. To make the color
bind and become permanent, a mordant is required, and
alum was used for this in both Mexico and South America.
De Sahagún (1829) reported that alum (tlaxocotl ) was well
known in Mexico; other additives were also used as reinforcing agents with cochineal or to vary the shades of red
(Donkin 1977).
Cochineal was also used as a paint for articles ranging
from houses (Zoque Indians in Chiapas) to the famous
Mixtec codices. It was employed by Indian women as a
cosmetic, and possibly had pharmaceutical and culinary
uses (Dahlgren 1963; Brana 1964; Donkin 1977). Cochineal
and silk production flourished in New Spain during the
16th century, although competition occurred between the
two insect cultures, particularly in the Mixteca Alta region
of Oaxaca. Main producing areas continued to be Oaxaca
and Puebla, and at the urging of governor Gómez de
Cervantes, Tlaxcala became an important producer. Before
1600, proposals for a royal monopoly of cochineal production in New Spain were circulating. Production in
Tlaxcala and Puebla, however, dropped in the mid-17th
century. After 1650, Oaxaca was described as the main supplier, and, by the end of the 18th century, Oaxaca was the
only significant producer of fine cochineal (Donkin 1977).
Decline and Resurgence
In the 1860s, the introduction of aniline dyes, stronger and
faster binding than cochineal, apparently sounded the
death knell for the insect dye. France, in 1860, and soon
thereafter, the United States, authorized the use of synthetic colorants in foods. In the early 1880s, mineral colorants, including lead-based pigments, began to be used in
foods and cosmetics. However, toward the end of the 19th
century, medical problems arising from dye use began to be
detected. For this reason, in the late 1890s and early 1900s,
several European countries, including Italy, Germany,
France, and Belgium, as well as Australia and the United
States, promulgated regulations for the control and use of
certain colorants in food. During 1950 to 1980, various synthetic colorants were decertified. As a result of these prohibitions, interest in natural colorants was renewed. The
natural red dyes belonging to three pigment groups
(carminic acid, anthocyanin, and betalains) were allowed
to bypass the certification process by the United States
Federal Drug Administration Agency (von Elbe 1977).
In 1974 possible toxicological effects of cochineal
carmine were reported. In response, the Joint FAO/WHO
Expert Committee on Food Additives demanded testing of
the colorant for toxicity, and urged lower levels in food
products. In 1976, the use of carmine was only permitted
in alcoholic beverages and only for a limited time, thus
precipitating a price drop for cochineal and carmine. At
the request of Peru, the principal producer country, and of
the FAO/WHO, the British Industrial Biological Research
Association (BIBRA) undertook a series of investigations
to determine effects of carmine in food and cosmetics.
Several studies were undertaken in 1980 through 1982, including tests of teratogenicity and embryotoxicity as well
as effects on multigenerational reproduction in rats (Ford
et al. 1987; Grant and Gaunt 1987; Grant et al. 1987). On
the basis of these results, in 1982 the FAO/WHO renewed
authorization of carmine and its derivatives. Certain imprecision in the BIBRA studies, as well as cases of reactions
to carmine in persons with allergies, have led to more recent studies with contradictory results: adverse effects
were reported by Quirce et al. (1994), whereas innocuousness was indicated by Kawasaki et al. (1994) and many others. The pros and cons of carmine use have had a significant impact on the prices of this pigment in the world
market.
Importance and Uses
The main coloring element of cochineal is carminic acid
(Fig. 13.7); secondary elements include kermesic and
flavokermesic acid (Wouters 1990) and minor pigments
(Sugimoto et al. 1998). Carminic acid is used today in the
industrial production of cosmetics, food, and medicines, as
well as in textile and other dyeing, although azo synthetic
dyes have, to a great extent, replaced the natural dyes in the
latter two categories. Tütem et al. (1996) have also reported possible therapeutic uses. Moreover, cochineal, in powdered form, is utilized in food products and especially in
the dyeing of textiles in countries such as Iran and Iraq
and, in Mexico, among the Zapotec Indians (Ross 1986).
Color and hue in textiles depend upon the mineral salts or
reagents with which the powder is mixed (Avila and
Remond 1986).Fgiure137.neahrere:
As an aqueous or alcohol extract, cochineal is an important colorant in food and beverages; as carmine, it is of
importance in cosmetology, medicine, and food products.
Carmine is marketed in a number of commercial presentations, including carminic acid at 90% (used as a colorant
in processed food products and soft drinks) and lakes (pur-
CH2OH
O
H
H
H
OH
H
OH
O
CH3
HO
COOH
H
HO
HO
OH
OH
O
Figure 13.7. Structure of carminic acid.
plish red pigments) composed of carminic acid at concentrations of 40 to 65% combined with a variety of inorganic carriers, of which aluminum and calcium are the most
common. Carmine 50 is the most sought-after commercially, e.g., as an additive to acidic beverages.
Carminic Acid
The physical and chemical properties of carminic acid were
studied by Lieberman and his associates in 1909 and by
Dimroth and colleagues from 1909 to 1920. The molecular structure of carmine was first proposed by Miyagawa
and Justin-Mueller in 1920s, with further evidence provided by Fieser and Fieser in 1944 and by Hay and Haynes
in 1956 and 1958 (Ali and Haynes 1959). Carminic acid is
a hydroxyanthraquinone linked to a glycosyl group (Fig.
13.7). Lac dye and kermesic acid (Old-World insect-derived
red dyes) have the same basic chemical structure but without the glycosyl linkage. Glycosyls apparently enhance the
intensity of the anthraquinone group in the visible part of
the spectrum. The chemical formula of carminic acid is
7β-D-glucopyranosyl-9,10-dihydro-3,5,6,8-tetrahydroxy-1methyl-9,10- dioxo-2 anthracene carboxylic acid (Fiecchi et
al. 1981). The colorant is listed in the Chemical Index as
Natural red 75470. Carmine is an aluminum chelate of this
molecule at a rate of 1:2 aluminum:carminic acid. The
great resistance of carminic acid to hydrolysis is due to the
glucosyl group. According to Hay and Haynes (1956,
1958; cited in Ali and Haynes 1959), the glucosyl can be Darabinose, which lends it a pink color, or glucose, which
provides a yellow color. There are many commercial products on the market, so chemical and physical properties are
asssigned by the firms. Carminic acid precipitates from alkaline solutions as prism-shaped crystals, obliquely truncated and red; they turn reddish-orange in the presence of
light. The Merck Index describes their characteristics as:
Nopalitos, Mucilage, Fiber, and Cochineal
223
(1) no distinct melting point; (2) darken at 120°C; and
(3) deep red color in water and yellow to violet in acid
solutions.
For the fabrication of carmine and its derivatives, Avila
and Remond (1986) review 12 of the many extraction and
purification techniques commonly employed. All techniques follow the principles established by De la Rue,
Lieberman, Dimroth, and others. In particular, the processing steps are: (1) separation of fats; (2) extraction of the
coloring agent by steeping in a solvent; (3) salt precipitation, which also varies according to the technique employed; and (4) separation, washing, concentration, and
drying of the final product. ITINTEC (1990) published a
detailed methodology to obtain a carmine extract with a
carminic acid content of 62 to 64%: (1) cleaning of insect
using a screen; (2) separation of fats with hexane; (3)
milling; (4) water extraction (100°C for 10 minutes)
adding sodium carbonate until the pH is 9.0; (5) screening
and filtering; (6) precipitation (aluminum and calcium
salts, 100°C for 15 minutes); (7) pH modification (4.8–
5.4); (8) decanting for 2 hours; (9) centrifuging or press
filtering; (10) washing with deionized water; (11) drying
(40°C) to achieve a water content of 7 to 10%; and (12)
packaging in polyethylene bags. Acetone can be used for
the separation of fats, an ethanol-water solution for their
extraction, and a concentration operation done before
filtration (Pérez 1992). To obtain essentially pure carminic
acid (99.5%), the main operation is crystalization, beginning with a supersaturated solution.
The extraction of dye from the insect is generally made
without any other aid but pure water ( J. A. Bustamante,
personal communication). The water should be near boiling, and the dye is extracted from dried insects using two
or three extractions (70:1 H2O:cochineal). The resulting
fluid is filtered, usually through a press filter, to obtain a
solid-free extract (the pH is often reduced to 4.5 with hydrochloric acid). The carminic dye solution can either be
spray-dried or laked with aluminum-calcium salts in the
presence of citric acid at a high temperature, and then allowed to cool and precipitate in the form of insoluble,
bright-red carmine lake particles. The lake is then dried in
a low-temperature oven and later milled to the customer’s
particular requirement.
Biology of Cochineal
Despite some controversy, cochineal apparently belongs to
order Homoptera, suborder Stenorryncha, and superfamily Coccoidae, which includes all mealy bugs and scale
insects (Gullan and Kosztarab 1997). The family Dactylopiidae belongs to the Neococcoidea group of the Coc-
224
Sáenz-Hernández, Corrales-García, and Aquino-Pérez
coidae (Miller and Kosztarab 1979). Named by Ferris
(1955), it comprises the genera Apezcoccus, Cryptococcus,
Dactylopius, Eriococcus, Gymnococcus, Kermes, Oncerotyga,
Trachiococcus, and Xerococcus. De Lotto (1974) has identified nine species in the genus Dactylopius: Dactylopius
autrinus (De Lotto), D. ceylonicus (Green), D. coccus
(Costa), D. confertus (De Lotto), D. confusus (Cockerell),
D. opuntieae (Cockerell), D. salmianus (De Lotto), D. tomentosus (Lamarck), and D. zimmermani (De Lotto).
Dactylopius ceylonicus, D. coccus, D. confusus, D. opuntieae,
and D. tomentosus are abundant in the southwestern
United States, Mexico, and northern South America
(Miller 1976; Macgregor L. and Sampedro R. 1984).
Origin and Diversity
The center of origin and dispersal of the various species of
cochineal have not been unambiguously determined.
However, the place or places of origin undoubtedly are intimately related to the development and diffusion of the
genera Opuntia and Nopalea. The cacti apparently originated in the neotropical regions of the Americas (southern
Mexico and northern South America), and then spread
both northward and southward (Gibson and Nobel 1986;
Nobel 1998). Maximum diversity is found from southern
Mexico to the southwestern United States. Although many
platyopuntias are important for fruit and forage, Opuntia
ficus-indica (L.) Miller is the species of greatest economic
importance and is the most useful host for cochineal
(Borrego and Burgos 1986). Although the range of nopal
species and varieties that serve as host to the cochineal insect is ample—75, according to Portillo (1995)—those that
function as natural hosts to D. coccus are few: notably, O.
ficus-indica var. Castilla, O. pilifera Weber, O. sarca Griff,
O. tomentosa Salm-Dyck, and Nopalea cochenillifera (L.)
Salm-Dyck (Piña 1977, 1981). A number of species and varieties can, however, be artificially adapted as hosts, including several varieties of O. amyclaea Webber, O. atropes
Rose, O. jaliscana Bravo, O. megacantha Salm-Dyck, and
O. streptacantha Lemaire. In South America, D. coccus is
reared on both spiny and spineless varieties of O. ficusindica (Flores 1995; Tekelemburg 1995).
The greatest diversity of natural enemies of the nopal
cactus is found in Mexico, among which are the different
species of Dactylopius. Unfortunately, however, detailed
studies of the full potential and diversity of the cochineal
insect in Mexico do not exist, compared with the meticulous investigations of the parasite by Ferris (1955) and
Gilreath and Smith (1988) for North America and South
America and by De Lotto (1974) for South Africa. Furthermore, the wide diversity of predators and parasitoids that
TA B L E 1 3 . 2
Life stage durations for Dactylopius coccus
Male
Stage
Female
Duration (days)
Emigrant nymph I
Cocoon construction
Prepupa
Nymph II
Pupa
Adult
Number of females mated
Life duration
15–168
2–64
8–22
2–12
4–8
18–29
0–3
50–89
Stage
Emigrant nymph I
Nymph I
Nymph II
Mating period
Sexual maturity
Pre-laying period
Laying period
Life duration with mating
Life duration without mating
Duration (days)
15–168
12–64
8–25
2–15
3–22
24–68
10–58
64–150
34–52
References: de Piña (1977); Marín and Cisneros (1977); Quispe (1983); Bustamante (1986); Vargas
(1988); Velasco (1988); Cruz (1990); Méndez (1992); Montiel (1995).
prey on both wild cochineal and D. coccus remain to be
examined.
Morphology and Life Cycle
Like other coccids, cochineal is characterized by sexual reproduction and sexual dimorphism. From the nymph I
stage to the first molt, it is difficult to distinguish between
the male and the female (Gunn 1978). Cytogenetically,
however, males and females are quite different from the
embryonic stage onward (Nur 1989; Aquino P. et al. 1994),
as females have a diploid chromosome number and males
are physiologically haploid. Paternal chromosomes, which
look like a heterochromatic mass, are known as H chromosomes (Nur 1989). These chromosomes are inactive, but
their presence is necessary for embryo viability, fertility,
and sexual differentiation (Chandra 1962).
The morphology and life cycle of D. coccus have been
extensively studied (Table 13.2). The male has a six-stage
life cycle (egg, nymph I, nymph II, prepupa, pupa, adult),
while the female has only four stages (egg, nymph I,
nymph II, adult). Life cycle duration, morphology, and
size of D. coccus are variable, depending on many factors,
such as population density, host species, nutritional level of
the host, soil quality where the host grows, light, and temperature. Despite this variability, certain characteristics are
common. The egg is oval and shiny bright-red. Hatching
may occur inside the adult female, or the crawlers may
hatch 10 to 32 minutes after the eggs are laid. Nymph I is
red and oval when recently hatched, but after a time becomes covered by a white powdery wax called coccicerin,
characteristic of the species.Tabe13l2.neahrere:
From 2 to 12 days after the first molt, the male as
nymph II, now clearly distinct from the female, constructs
a cocoon of filamentous wax. (Wild cochineal males build
their cocoons on the same day as the molt.) In the prepupa stage, the antennae are relatively thick and curved backward, genitalia are distinguishable, and most meiotic activity and the greatest development of the testicles occur.
In the pupa, the antennae have grown; the legs are now
long and slender and lack nails. Dorsally, the segmentation
of the abdomen can be seen, and ventrally the genitalia can
be observed. The adult male is approximately 2.2 mm in
length with a wingspan of 4.8 mm (wild cochineal males
are considerably smaller). The body sections are clearly distinguishable. The antennae are moniliform (tapering),
composed of 10 beadlike segments. Other features of the
adult include: three pairs of simple eyes; no buccal apparatus; a pair of simple-veined, mid-thoracic wings; slender
legs with a single nail; and a pair of white, waxy appendages, measuring up to 3.6 mm in length. Cruz (1990)
reports that a male mates with only one to three females;
reproductive efficiency is considerably reduced after more
than two matings.
The nymph II female does not change form; the duration between nymph II and adult varies from 8 to 25 days
(Table 13.2). The adult female is oval, 4 to 6 mm long, and
3 to 5 mm wide. Segmentation of the prosoma is barely evident (De Lotto 1974). Legs and antennae are well developed but small. The rostrum is made up of three segments
(De Lotto 1974; Montiel 1995). The body has numerous
(15) cuticular pores, both on the dorsum and on the abdomen, this being the characteristic commonly used by
Nopalitos, Mucilage, Fiber, and Cochineal
225
taxonomists to classify the species (Ferris 1955; De Lotto
1974). The anal ring is recognized as a modification of segment 10; it is dorsal, has a half-moon shape, and is membranous on the anterior margin and sclerotic on the posterior margin (De Lotto 1974; Montiel 1995). Receptivity
or sexual maturity occurs within 3 to 22 days. Pre-oviposition lasts from 24 to 68 days and oviposition is from 10
(when separated from the host) to 58 days. The total biological cycle of the female lasts from 64 to 150 days; during
that time, a female may lay over 400 eggs.
Although it is not clear whether parthenogenetic reproduction is possible for Dactylopius spp., sexual reproduction is necessary for D. coccus and D. confusus. Hence,
the ratio between the sexes should be 1:1 (Cruz 1990;
Gilreath and Smith 1987). However, the ratio is modified
by temperature (Mendez 1992) and photoperiod (Montiel
1995), generally favoring the female (Marín and Cisneros
1977). At least for D. ceylonicus and D. coccus, weather
changes, especially low temperature and drought, as well as
population density, affect the male population.
Host
The age of the cladodes selected and their utility for
cochineal production depend on the species and variety of
nopal, environmental conditions, and especially the hydration of the cladode, the quality of the soil, and the relative humidity. One-year-old, peripheral cladodes provide
the most favorable conditions for cochineal establishment
(Flores 1995). Five or six different kinds of nopal were utilized in Mexico for cochineal production in the 16th century. In time, most of these varieties were introduced into
the West Indies and Europe. The most important nopal
species for the raising of cochineal has always been O. ficusindica, with its different varieties; Nopalea cochinellifera has
also been used, but not in Mexico (Donkin 1977).
Nutritional levels of nopal also affect the establishment and
productivity of cochineal and its carmine content (Arteaga
1990; Vigueras and Portillo 1995). Indeed, oxalate crystals
in host tissue can influence the population of cochineal
(Castillo 1993).
Spines apparently have no influence on the establishment of D. coccus. Nevertheless, Piña (1981) indicates that,
both in Mexico and in South America, cochineal occurs
naturally on spiny nopal species, and under cultivation, the
spiny species are more productive. Portillo (1995) reaffirms
the greater adaptability of D. coccus to spiny varieties.
Nevertheless, the nopal cacti most commonly utilized for
the rearing of cochineal are spineless, for the obvious advantage of plant management. Only a weak relationship
exists between the establishment of cochineal insects and
226
Sáenz-Hernández, Corrales-García, and Aquino-Pérez
stomatal frequency or the thickness of the cuticle, epidermis, and hypodermis (Castillo 1993).
Environmental Factors and Natural Enemies
Data from Mexico and other parts of the world indicate
that environmental factors influence the growth and development of the cochineal insect (Piña 1977, 1981; Cruz
1990; Méndez 1992; Flores 1995; Tekelenburg 1995; Table
13.3). Important climate hazards include frosts, high temperatures (above 30°C), rainfall with its washing effects,
and wind, all of which restrict the establishment of nymph
colonies on the host plants. Biological hazards of the
cochineal insect include predators and parasitoids, both for
natural populations of wild cochineal (Gilreath and Smith
1988; Eisner et al. 1994) and for cultivated cochineal (Piña
1977). Such information can improve biological control of
wild cochineal by means of its predators, as well as control
of the predators by means of their parasitoids for cultivated cochineal.Tabe13ln.eahrere:
Similar predators attack D. coccus and D. confusus, considerably reducing dye yields (Alzate 1831; Dahlgren 1963).
The most common and dangerous predators are Hyperaspis
sp. (Coccinelidae, Coleoptera), Chilocorus spp. (Coccinelidae), “drum worm” (Bacca sp., Syrphidae: Diptera),
Laetilia sp. (Pyralidae: Lepidoptera), Sympherobius sp.
(Neuroptera), and Sapingogaster texana (Syrphidae; Piña
1977; Gilreath and Smith 1988). Homalotylus cockerella is a
parasitoid of Hyperaspis trifurcata (Gilreath and Smith
1988). In Mexico, several parasitoids of the family
Pteromalidae have been identified for Sapingogaster texana
(Syrphidae) — Brachimeria conica, Spilochalcis flavopicta
(Chalcididae), and Temelucha sp. (Ichneumonidae;
Gilreath and Smith 1988) — and mites belonging to the
family Piemotidae have been identified as parasitoids for
Laetilia sp. In greenhouse rearings, some parasitoids
(Pteromalidae) attack both cochineal and its predators
(Hyperaspis sp.).
D. coccus versus Wild Cochineal
Two characteristics distinguish D. coccus from wild cottony
cochineal. First, the wild insects produce a waxy, cottonlike
coating, which is abundant, loose, soft, and thermally stable on the dorsal side, but compact and resistant on the
ventral side, permitting firm adherence of the insect to the
host platyopuntia; this coating makes the parasite resistant
to wind and rain. Second, wild cochineal yields a much
lower concentration of carmine colorant, from 2 to 7% by
dry weight, whereas yields from D. coccus are 15 to 25%.
Among other characteristics of the wild species is the ability to transmit disease to the host (Miller 1976), which in
TA B L E 1 3 . 3
Environmental parameters influencing D. coccus
Factor
Range
Optimal
Mean temperature (°C)
20–32
26–28
Light (% of maximal)
40–60
50
Relative humidity (%)
Annual rainfall (mm)
30–60
0–600
40
0–100
Altitude (m)
0–2,300
200–1,800
Soil type
Alluvium
Volcanic
addition to its short biological cycle, high reproductive potential, and high resistance to climatic factors, makes wild
cochineal very useful in the control of weedy nopal
(Zimmermann and Moran 1991; Chapter 14). However, it
is also a dangerous competitor of D. coccus. Dactylopius coccus, on the other hand, is distinguished by its fine, waxy
coating, which takes the form of a powder. Apart from its
buccal apparatus, D. coccus has no other structure that aids
its adherence to the surface of a cladode, making it more
susceptible to environmental hazards.
Cytogenetically, all analyzed wild cochineal species
have a chromosome number of n = 5 and similar chromosomal karyotypes: a long chromosome three times the average length and four short chromosomes (Aquino P. et al.
1994). Dactylopius coccus has a chromosome number of
n = 8 and a different karyotype—all the chromosomes are
short. The four longest chromosomes of D. coccus do not
differ from those of their possible homologue in wild
cochineal, and the length of the long wild cochineal chromosome equals the length of the sum of the four shortest
chromosomes of D. coccus (Aquino P. et al. 1994). Thus,
cochineal insects apparently eliminate meiosis II by a simple chromosomal segregation. Dactylopius coccus has a very
low frequency of “normal” meiosis II, whereas wild species,
such as D. confusus, have a high frequency of “normal”
meiosis II (Aquino P. et al. 1994).
Coccidae generally have evolved toward an increase in
chromosome number by means of chromosome fragmentation (Nur et al. 1987; Nur 1989). This has apparently occurred for both D. coccus and wild cottony cochineal.
Nevertheless, a chromosome breakup usually produces
more disadvantages than advantages, and three fragmen-
Observations
Cochineal can tolerate temperature extremes below 0°C or above
36 to 40°C; the effects depend on the duration of exposure.
Can be controlled by shading; the amount of light required interacts
with the temperature.
Estimated, as parameters are not fully studied.
Depending on the temperature, cochineal can be raised in areas
of greater or lesser rainfall, but with protection and, if necessary,
irrigation.
Not a limiting factor, if temperature and radiation are in the
appropriate ranges.
Limestone soils, or soils with high calcium or magnesium contents,
are apparently not favorable.
tations (which is what presumably occurred for D. coccus)
could make the survival of the progeny more difficult.
However, two additional modifications in the chromosomes of this insect group would increase the possibilities
of survival of mutant individuals: (1) the presence of a
diffuse centromere and (2) the inversion of the meiotic sequence (Chandra 1962). This permits balanced meiosis and
equal separation of the chromosome groups, thereby reducing the loss of chromosome fragments—an irreconcilable loss in species with a defined centromere.
Marketing of Cochineal
Production and Prices
Since pre-Hispanic times in America, the cochineal market has been heavily affected by political and natural
events, which have had much influence on production and
prices. The highest prices for cochineal have always coincided with periods of prosperity in the principal consumer
countries — historically England, France, Germany, and
Holland, and at present, Italy, Japan, and the United States
(Alzate 1831; Houghton 1877; Dahlgren 1963; Brana 1964;
Donkin 1977; EPTASA 1983; Avila and Remond 1986;
Contreras S. 1996; P. Quintanilla, personal communication; J. A. Bustamante, personal communication). The
most favorable prices for cochineal possibly occurred from
1765 to 1775, when 520 metric tons (1 ton = 1,000 kg) were
produced annually; the period from 1799 to 1833 was also
a time of bonanza, although annual worldwide production
had dropped to 190 tons, again almost all from Mexico.
After this period, production and prices began to drop in
Mexico in response to fierce competition from 1825 to 1880
Nopalitos, Mucilage, Fiber, and Cochineal
227
from Guatemala and the Canary Islands. The Guatemalan
market flourished from 1830 to 1865, but was overtaken by
the booming Canary Islands’ production from 1849 to
1882, despite the fact that world prices for cochineal had
been in gradual decline since 1831. The main advantage for
the Canary Islands was probably geographic—proximity to
the main European consumer countries. Soaring production and lower shipping prices of Canary Islands’ cochineal
were probably the reasons for the drop in demand for the
Guatemalan and Mexican dye; South American cochineal,
meanwhile, had been in a slump since the turn of the 17th
century. The Canary Islands’ production continued from
1880 to 1927, albeit at lower levels and lower prices, due to
the introduction of synthetic dyes; annual production averaged approximately 80 tons for 1927 to 1929 and currently is 40 tons year-1.
Although little information is available for South
American cochineal production for 1750 to 1935, production was apparently over 1,000 tons for 1829 to 1859 (Brana
1964; Donkin 1977). Records of the Peruvian market appear in 1937 and show a linear increase since that date, becoming 100 tons by 1975 (Fig. 13.8A). From 1975 to 1990,
Peru cornered the world market, with a share of up to 95%.
Since 1990, Argentina, Bolivia, and Chile have entered the
world market, causing a reduction in Peru’s domination.
Indeed, Peru’s bulk exportation of the raw material since
1997 has diminished, and a greater volume is being consigned to processed dye (Fig. 13.8).Fgiure138.neahrere:
Supply and Demand
Presently Peru is the world’s most important producer of
cochineal. In response to increasing demand, Peru’s production increased sixfold from 1975 to 1998, reaching 650
tons (Fig. 13.8A). Since 1983, Peru has steadily increased its
industrial processing capacity for cochineal; since 1986
most of the exported cochineal has been processed (Fig.
13.8A). The price of cochineal has fluctuated widely, generally being below U.S. $20 per kilogram but reaching $40
in 1985 and $75 in 1996 (Fig. 13.8B). Prices of refined
carminic acid, which accounts for about 10% of the dye
export, tripled from 1994 to 1996, when it reached $400
per kg then decreased twofold by 1998, indicating the
volatility of the market. If competition from synthetic dye
manufacturers can be surmounted and if cochineal dye is
internationally certified, the demand should increase.
Chile entered the world market in 1997 with a production of approximately 150 tons. Traditionally, Spain had
5 to 10% of world production, or approximately 65 tons.
Peru, Chile, and Spain recently have annually supplied the
world marketplace with 755 tons of cochineal. Moreover,
228
Sáenz-Hernández, Corrales-García, and Aquino-Pérez
Figure 13.8. Peruvian cochineal production and prices for 1975
to 1998: (A) total production (0) and exportation of processed
cochineal (∆), and (B) prices for processed cochineal. References:
Avial and Redmond (1986); EPTASA (1983); Contreras S. (1996);
P. Quintanilla (personal communication); J. A. Bustamante
(personal communication).
Chile and Peru have predicted increases in production,
meaning that the annual supply of the dye from these three
countries should approach 1,000 tons.
Conclusions and Future Prospects
A common way to consume cladodes in Mexico is as
nopalitos. After despining, minimal processing facilitates
consumption of the tender young pads. Nopalitos can be
further processed in brine or pickled. The consumption of
the young pads not only should increase the cultivation of
Opuntia ficus-indica and consequently the use of arid lands
in many regions of the world, but also should serve as a
healthful food due mainly to the dietary fiber content.
Dietary fiber increases with stem age, opening up other
ways to process and use this part of platyopuntias in addition to the currently more popular use of young pads. Such
use could be introduced into those countries where O.
ficus-indica is presently only a fruit crop. The different alternatives for processing and consuming nopal and nopalitos require educating the consumers, including full information on the nutritional value of the cladodes and
technology transfer. The processes used today are quite
simple and do not require expensive equipment. The food
industry can utilize similar processes already installed for
other raw vegetables. The properties of cladodes to alleviate illnesses, such as diabetes and obesity, should be studied more to confirm their effectiveness. Mucilage has
great potential as a thickener in foods and an adhesive in
paints, but again these properties must be studied in
greater detail.
The dye insect Dactylopius coccus has enjoyed great importance worldwide since its discovery in Mexico in the
16th century. Cochineal is valued not only as the source of
a red colorant, useful in a number of products for human
consumption, but also as a biological control of weedy
nopal infestations in some parts of the world. Its importance as a colorant has made it the subject of scientific, economic, and historical inquiry since the late 18th century.
The most advanced investigations undertaken so far have
been historical, chemical, and toxicological, whereas the biology of the parasitic insect has been largely ignored until
recently. Genetic and biosynthetic aspects, as well as the
host (Opuntia spp.)–parasite (Dactylopius spp.) interaction,
have not received enough attention ( Joshi and Lambdin
1996). For these reasons, it is presently difficult to determine
the phylogenetic and evolutionary relationships, as well as
the agronomic techniques, that would maximize the potential of both susceptible host cacti and D. coccus.
Acknowledgments
The authors thank anthropologist Jodie Randall for her
comments regarding cochineal history and Mayra Perez
Sandi y Cuen (Mac Arthur Foundation) for providing information on the cochineal trade.
Literature Cited
Albornoz, N. 1998. Elaboración y Evaluación de una Crema
de Verduras con Adición de Fibra Dietética de Nopal.
Facultad de Ciencias Agrarias y Forestales. Universidad
de Chile, Santiago.
Ali, M. A., and L. J. Haynes. 1959. C-Glycosyl compounds. Part III. Carminic acid. Journal of the Chemical Society: 1033–1035.
Alzate, J. A. 1831. Memoria acerca del insecto grana o cochinilla, su naturaleza y serie de su vida, método de
propagarlo y reducirlo al estado en que forma uno de los
ramos más útiles del comercio (Written in 1777). Imprenta del Aguila, Mexico City.
Amin, El-S., O. M. Awad, and M. M. El-Sayed. 1970.
The mucilage of Opuntia ficus-indica. Carbohydrate
Research 15: 159–161.
Aquino, P., G., A. García V., T. Corona T., and N. M.
Bárcenas O. 1994. Estudio cromosómico de cuatro especies de cochinilla del nopal (Dactylopius spp.) (Homoptera: Dactylopiidae). Agrociencia, serie Fitociencia
5: 7–23.
Arteaga, J. L. 1990. Influencia de la fertilización N-P-K en
la producción de cochinilla (Dactylopius coccus Costa).
Bachelor’s Thesis, Universidad Nacional de San
Cristóbal de Huamanga, Ayacucho, Peru.
Avena, B. R. S. 1996. Proceso de hortalizas pre-cortadas.
Boletín del Centro de Investigación en Alimentos y
Desarrollo, A.C. 5: 7–8.
Avila, R., and Z. Remond. 1986. Estudio Técnico de la
Cochinilla. Dirección de Servicios Técnicos, División
de Extracción Industrial (ITINTEC), Lima, Peru.
Badillo, J. R. 1987. Elaboración de una jalea de nopal.
Bachelor’s Thesis, Escuela de Ciencias Químicas,
Universidad Autónoma de Puebla, Puebla, Mexico.
Barbera, G. 1991. Utilizzazione economica delle Opunzie
in Messico. Frutticoltura 2: 41–48.
Borrego, F., and N. Burgos. 1986. El Nopal. Universidad
Autónoma Agraria Antonio Narro, Saltillo, Mexico.
Brana, D. D. 1964. Cochineal: Aboriginal dyestuff from
Nueva España. In Actas y Memorias del XXXVI Congreso Internacional de Americanistas. University of
Texas, Austin. Pp. 77–91.
Brecht, J. K. 1995. Physiology of lightly processed fruits
and vegetables. HortScience 30: 18–24.
Bustamante, O. 1986. Estudio del ciclo biológico de la cochinilla (Dactylopius coccus Costa) en su ambiente natural en Ayacucho. In Resúmenes del 1er. Congreso
Nacional de Tuna y Cochinilla. Ayacucho, Peru. Pp.
44–45.
Cantwell, M. 1991. Quality and postharvest physiology of
“nopalitos” and “tunas.” Proceedings of the 2nd Annual
Texas Prickly Pear Conference. McAllen, Texas. Pp. 50–
66.
Cantwell, M. 1992. Postharvest handling systems: Minimally processed fruits and vegetables. In Postharvest
Technology of Horticultural Crops (A. A. Kader, ed.).
Division of Agriculture and Natural Resources,
Publication 3311. University of California, Davis. Pp.
227–281.
Cantwell, M. 1995. Post-harvest management of fruits and
vegetable stems. In Agro-ecology, Cultivation and Uses of
Cactus Pear (G. Barbera, P. Inglese, and E. PimientaBarrios, eds.). FAO Plant Production and Protection
Paper 132. FAO, Rome. Pp. 120–136.
Nopalitos, Mucilage, Fiber, and Cochineal
229
Cantwell, M., A. Rodríguez-Felix, and F. RoblesContreras. 1992. Postharvest physiology of prickly
pear cactus stems. Scientia Horticulturae 50: 1–9.
Cárdenas, A., and F. Goicoolea 1997. Reología en solución
del mucílago del nopal (Opuntia ficus-indica). In
Memorias VII Congreso Nacional y V Internacional
Conocimiento y Aprovechamiento del Nopal. Monterrey,
Mexico. Pp. 171–172.
Cárdenas, A., I. Higuera-Ciapara, and F. M. Goicoolea.
1997. Rheology and aggregation of Cactus (Opuntia
ficus-indica) mucilage in solution. Journal of the Professional Association for Cactus Development 2: 152–159.
Castillo V., J. 1993. Relación entre algunas características
anatómicas y químicas del nopal (Opuntia sp.) y el establecimiento de la cochinilla (Dactylopius coccus
Costa). Bachelor’s Thesis, Departamento de Fitotécnia,
Universidad Autónoma Chapingo, Chapingo, Mexico.
Chandra, H. S. 1962. Inverse meiosis in triploid female of
the mealy bugs, Planococcus citri. Genetics 47: 1441–
1454.
Chang, L. A., L. K. Hammet, and D. M. Pharr. 1982.
Ethanol, alcohol dehydrogenase and pyruvate decarboxylase in storage roots of four sweet potato cultivars
during simulated flood damage and storage. Journal of
the American Society for Horticultural Science 107: 674–
677.
Colin, B.C. 1976. Industrialización del nopal y sus productos. Tecnología Laboratorios Nacionales de Fomento
Industrial II: 11–13.
Contreras S., A. C. 1996. Capital Comercial y Colorantes en
la Nueva España: Segunda mitad del siglo XVIII. El
Colegio de Michoacán y Universidad Autónoma de
Yucatán, Mérida, Mexico.
Corrales, G. J. 1992a. Descripción y análisis de la cosecha y
del manejo en fresco de nopalito y tuna. In Memoria de
Resúmenes del 5to. Congreso Nacional y 3er. Congreso
Internacional Conocimiento y Aprovechamiento del Nopal.
Universidad Autónoma de Chapingo, Mexico. P. 109.
Corrales, G. J. 1992b. Perspectivas de industrialización de
nopalito y tuna. In Memoria de Resúmenes del 5to. Congreso Nacional y 3er. Congreso Internacional Conocimiento y Aprovechamiento del Nopal. Universidad Autónoma de Chapingo, Chapingo, Mexico. Pp. 97–98.
Corrales-García, J. 1997. Postcosecha de la tuna y del nopal
verdura. In Memorias del VII Congreso Nacional y V
Internacional Conocimiento y Aprovechamiento del
Nopal. Facultad de Agronomía, Universidad Autónoma de Nuevo León, Monterrey, México. Pp. 88–98.
230
Sáenz-Hernández, Corrales-García, and Aquino-Pérez
Corrales-García, J. 1998. Industrialization of prickly pear
pads (nopalitos). In Proceedings of the International
Symposium Cactus Pear and Nopalitos Processing and
Uses. Universidad de Chile, Santiago. Pp. 25–32.
Cruz, M. 1990. Determinación de algunos aspectos biológicos de la grana-cochinilla del nopal (Dactylopius
coccus Costa) (Coccoidea: Dactylopiidae) en Chapingo,
México. Bachelor’s Thesis, Universidad Autónoma de
Chapingo, Chapingo, Mexico.
Dahlgren, B. 1963. La Grana o Cochinilla. Nueva Biblioteca Mexicana de Obras Históricas Vol. I, Editorial José
Porrúa Hermanos, Mexico City.
De Lotto, G. 1974. On the status of an identity of the
cochineal insect (Homoptera: Coccoidea: Dactylopiidae). Journal of the Entomological Society of South
Africa 37: 167–193.
De Sahagún, B. 1829. Historia general de las cosas de
Nueva España. In Sepan Cuantos (A. M. Garibay E.,
ed.). Editorial Porrua, Mexico City.
Donkin, R. A. 1977. Spanish red: An ethnogeographical
study of cochineal and the Opuntia cactus. Transactions
of the American Philosophical Society 67: 63–77.
Eisner, T., R. Ziegler, J. L. McCormick, M. Eisner, E. R.
Hoebeket, and J. Meinwald. 1994. Defensive use of an
acquired substance (carminic acid) by predaceous insect larvae. Experientia 50: 610–615.
EPTASA (Estudios de Planificación y Técnica Aplicada,
S. A.). 1983. Estudio sobre la producción y comercialización de cochinilla y carmín de cochinilla. Ministerio
de Agricultura Pesca y Alimentación, Madrid, Spain.
Fernández, M. L., A. Trejo, and D. McNamara. 1990.
Pectin isolated from prickly pear (Opuntia sp.) modifies low density lipoprotein metabolism in cholesterolfed guinea pigs. Journal of Nutrition 120: 1283–1290.
Ferris, G. F. 1955. Family Dactylopiidae Fernald. In Atlas of
the Scale Insects of North America. Stanford University
Press, Stanford, California. Pp. 69–217.
Fiecchi, A., M. Anastasia, G. Galli, and P. Gariboldi. 1981.
Assignment of the β configuration to the C-glycosyl
bond in carminic acid. Journal of Organic Chemistry
46: 1511.
Flores, V. 1995. Crianza de la cochinilla en Sudamérica. In
Memorias del 6to. Congreso Nacional y 4to. Congreso
Internacional sobre el Conocimiento y Aprovechamiento
del Nopal. Universidad de Guadalajara, Zapopan,
Mexico. Pp. 35–47.
Flores, V. C. 1997. La producción de nopalito en México.
In Memorias del VII Congreso Nacional y V Inter-
nacional Conocimiento y Aprovechamiento del Nopal.
Monterrey, Mexico. Pp. 28–38.
mation of Opuntia ficus-indica. Plant Physiology 97:
954–961.
Fontanot, M. 1999. Elaboración de galletas de avena con
adición de harina de nopal rica en fibra dietética.
Master’s Thesis, Universidad de Chile, Santiago.
Granados, D., and A. D. Castañeda. 1991. El Nopal.
Historia, Fisiología, Genética e Importancia Frutícola.
Editorial Trillas, Mexico City.
Ford, G. P., T. Gopal, D. Grant, I. F. Gaunt, J. G. Evans,
and W. H. Butler. 1987. Chronic toxicity/carcinogenicity study of carmine of cochineal in the rat. Food
and Chemical Toxicity 25: 897–902.
Grant, D., I. F. Gaunt, and F. M. B. Carpanini. 1987.
Teratogenicity and embriotoxicity study of carmine of
cochineal in the rat. Food and Chemical Toxicity 25:
913–917.
Frati-Munari, A. C., L. M. Del Valle-Martínez, C. R.
Araiza-Andraca, S. Islas-Andrade, and A. ChávezNegrete. 1989. Hypoglycemic effect of different doses
of nopal (Opuntia streptacantha) in patients with type
II diabetes mellitus. Archivos de Investigación Medica
20: 197–201.
Grant, D., and I. F. Gaunt. 1987. Three generation reproduction study on carmine of cochineal in rat. Food and
Chemical Toxicity 25: 903–912.
Frati-Munari, A., E. Jiménez, and C. R. Ariza. 1990. Hypoglycemic effect of Opuntia ficus-indica in non insulindependent diabetes mellitus patients. Phytotherapy
Research 40: 195–197.
Frati-Munari, A., O. Vera Lastras, and C. R. Ariza
Andraca. 1992. Evaluación de cápsulas de nopal en diabetes mellitus. Gaceta Médica de México 128: 431–436.
Gallardo, Y., M. L. Zambrano, and A. D. Hernandez.
1997. Determinación de las propiedades fisicoquímicas
del nopal verdura. In Memorias VII Congreso Nacional
y V Internacional Conocimiento y Aprovechamiento del
Nopal. Monterrey, Mexico. Pp. 277–278.
García, J. R. 1993. Análisis de Factibilidad Técnico,
económico y financiero de una planta procesadora de
nopal en escabeche. Bachelor’s Thesis, Escuela
Nacional de Ciencias Biológicas, Instituto Politécnico
Nacional, Mexico City.
Gardiner, D., P. Felker, and T. Carr. 1999. Cactus extract
increases water infiltration rates in two soils. Communications in Soil Science and Plant Analysis 30:
1707–1712.
Gibson, A. C., and P. S. Nobel 1986. The Cactus Primer.
Harvard University Press, Cambridge, Massachusetts.
Gilreath, E. M., and J. W. Smith Jr. 1987. Bionomics of
Dactylopius confusus (Homoptera: Dactylopiidae).
Annals of Entomological Society of America 80: 768–
774.
Gilreath, E. M., and J. W. Smith. 1988. Natural enemies
of Dactylopius confusus (Homoptera: Dactylopiidae)
exclusion and subsequent impact on Opuntia
(Cactaceae). Environmental Entomology 17: 730–738.
Goldstein, G., and P. S. Nobel. 1991. Changes in osmotic
pressure and mucilage during low-temperature accli-
Grijspaardt-Vink, C. 1996. Ingredients for healthy foods
featured at European expo. Food Technology 2: 30.
Gulías, A., and G. Robles. 1989. El nopal en su justa medida. Cuadernos de Nutrición 12: 42–43.
Gullan, P. J., and M. Koszarab. 1997. Adaptations in scale
insects. Annual Review of Entomology 42: 23–50.
Gunn, B. H. 1978. Sexual dimorphism in the first instar of
the cochineal insect Dactylopius austrinus De Lotto
(Homoptera: Dactylopiidae). Journal of the Entomological Society of South Africa 41: 333–338.
Hegwood, D. 1990. Human health discoveries with
Opuntia sp. (Prickly pear). HortScience 25: 1515–1516.
Hernández, A. D., G. Chamorro, and Y. Gallardo. 1997.
Efecto fisiológico de la ingesta de nopal sobre la producción de heces fecales. In Memorias VII Congreso
Nacional y V Internacional Conocimiento y Aprovechamiento del Nopal. Monterrey, Mexico. Pp. 289–
290.
Hernández, A. D., Y. Gallardo, and G. Chamorro. 1998.
Caracterización de la fibra de nopal por medio de su respuesta fisiológica. In Fibra Dietética (F. Lajolo and E.
Wenzel, eds.). Temas en Tecnología de Alimentos, Vol.
2. Instituto Politécnico Nacional, Mexico City. Pp.
215–230.
Hollingsworth, P. 1996. Food trends: Diversity and choice
dominate. Food Technology 5: 40.
Houghton, H. 1877. La Cochinilla: Memoria Demostrativa
de las Causas que han Producido la Decadencia de este
Renglón de Comercio en los Ultimos Años. Sociedad
Económica de Amigos del País, Gran Canaria, Spain.
Ibañez-Camacho, R., and R. Roman Ramos. 1979. Efecto
hipoglucemiante del nopal. Archivos de Investigación
Médica 10: 223–230.
Ibañez-Camacho, R., M. Meckes-Lozoya, and V. MelladoCampos. 1983. The hypoglycemic effect of Opuntia
Nopalitos, Mucilage, Fiber, and Cochineal
231
streptacantha studied in different animal experimental
models. Journal of Ethnopharmacology 7: 175–181.
mellitus. American Journal of Chinese Medicine 14: 116–
118.
ITINTEC (Instituto Nacional de Investigación Tecnológica Industrial y de Normas Técnicas, Perú). 1990.
Perfil Industrial: Producción de carmin a partir de la
cochinilla. Información Tecnológica 16: 33–36.
Méndez, G., S. J. 1992. Tasas de supervivencia y reproducción de la grana-cochinilla Dactylopius coccus Costa
(Homoptera:Dactylopiidae) a diferentes temperaturas.
Master’s Thesis, Colegio de Postgraduados, Chapingo,
Mexico.
Joshi, P. A., and P. L. Lambdin. 1996. The ultrastructure of
hemocytes in Dactylopius confusus (Cockerell), and the
role of granulocytes in the synthesis of cochineal dye.
Protoplasma 192: 199–216.
Kader, A. A. 1986. Biochemical and physiological basis for
effects of controlled and modified atmospheres on
fruits and vegetables. Food Technology 40: 99–104.
Kawasaki, Y., T. Umemura, K. Sai, R. Hasegawa, J.
Momma, M. Saitoh, Y. Matsushima, Y. Nakaji, M.
Tsuda, and Y. Kurokawa. 1994. A 13-week toxicity
study of simultaneous administration of cochineal
and aluminum potassium sulfate in rats. Bulletin of the
National Institute of Health Sciences 112: 48–56.
Lecaros, M. 1997. Caracterización de Harina de Cladodio de
Nopal (Opuntia ficus-indica). Universidad de Chile,
Santiago.
Loik, M. E., and P. S. Nobel. 1991. Water relations and
mucopolysaccharide increases for a winter hardy cactus
during acclimation to subzero temperatures. Oecologia
86: 340–346.
Macgregor L., R., and G. Sampedro R. 1984. Catálogo de
cóccidos mexicanos familia Dactylopiidae (HomopteraCoccidae). Anales del Instituto de Biología (Serie
Zoología), Universidad Nacional Autónoma de México
54: 217–223.
Marín L., R., and V. Cisneros F. 1977. Biología y morfología de la cochinilla del carmín (Dactylopius coccus
Costa) Homoptera: Dactylopiidae. Revista Peruana de
Entomología 29: 115–120.
McGarvie, D., and P. H. Parolis. 1979a. The mucilage of
Opuntia ficus-indica. Carbohydrate Research 69: 171–
179.
McGarvie, D., and P. H. Parolis. 1979b. The mucilage of
Opuntia ficus-indica. Part 2. The degraded polysaccharide. Journal of the Chemical Society, Perkin Transactions
1: 1464–1466.
McGarvie, D., and P. H. Parolis. 1981. Methylation analysis
of the mucilage of Opuntia ficus-indica. Carbohydrate
Research 88: 305–314.
Meckes, L. M., and R. Roman-Ramos. 1986. Opuntia
streptacantha: A coadjutor in the treatment of diabetes
232
Sáenz-Hernández, Corrales-García, and Aquino-Pérez
Miller, D. H. 1976. Dactylopiidae. In Syllabus for workshop
on scale insect identification. Proceedings of National
Meeting of the Entomological Society of America ( J. W.
Beradsley and U. Nur, eds.), Honolulu. Pp. 1–10.
Miller, D. R., and M. Kosztarab 1979. Recent advances in
the study of scale insect. Annual Review of Entomology
24: 1–27.
Montiel R., M. L. 1995. Morfología de Dactylopius coccus
Costa (Homoptera: Dactylopiidae) y su biología y
producción en dos fotoperiodos. Master’s Thesis,
Colegio de Postgraduados, Montecillo, Mexico.
Mulas, M. 1993. Medicinal properties and yield possibilities of the prickly pear (Opuntia spp.) in the mediterranean environment. Acta Horticulturae 331: 79–84.
Nerd, A., M. Dumotier, and Y. Mizrahi. 1997. Properties
and postharvest behaviour of the vegetable cactus
Nopalea cochenillifera. Postharvest Biology and Technology 10: 135–143.
Nobel, P. S. 1998. Los Incomparables Agaves y Cactos.
Editorial Trillas, Mexico City.
Nobel, P. S, J. Cavelier, and J. L. Andrade. 1992. Mucilage
in cacti: Its apoplastic capacitance, associated solutes,
and influence on tissue water relations. Journal of
Experimental Botany 43: 641–648.
Nur, U. 1989. Chromosomes, sex-ratios and sex determination: Reproductive biology and genetics. In Armored
Scale Insects, Their Biology, Natural Enemies and Control. Vol. A. (D. Rosen, ed.). Elsevier Science Publishers, Amsterdam. Pp.179–190.
Nur, U., S. W. Brown, and J. W. Beardsley. 1987. Evolution
of chromosome number in mealy bugs (Pseudoccidae:
Homoptera). Genetica 71: 53–60.
Paulsen, B. S., and P. S. Lund. 1979. Water-soluble polysaccharides of Opuntia ficus-indica cv. “Burbank’s
spineless.” Phytochemestry 18: 569–571.
Perez, J. A. 1992. Anteproyecto de una planta procesadora
de grana cochinilla para la obtención de sus principales
derivados: extracto de cochinilla, carmin de cochinilla
y acido carmínico. Bachelor’s Thesis, Universidad La
Salle, Mexico City.
Periago, M. J., G. Ros, G. López, M. C. Martinez, and F.
Rincón. 1993. The dietary fiber components and their
physiological effects. Revista Española de Ciencia y
Tecnología de Alimentos 33: 229–246.
changes of diced nopal during storage in polyethylene
bags. In Proceedings of the 3rd Annual Texas Prickly Pear
Conference, Texas Prickly Pear Council. McAllen, Texas.
Pp. 22–25.
Pimienta Barrios, E. 1990. El Nopal Tunero. Universidad de
Guadalajara, Zapopan, Mexico.
Rodríguez-Felix, A., and M. A. Villegas-Ochoa. 1998.
Postharvest handling of cactus leaves (nopalitos). In
Proceedings of the International Symposium “Cactus
Pear and Nopalitos Processing and Uses.” Universidad de
Chile, Santiago. Pp. 7–16.
Piña L., I. 1977. La Grana o Cochinilla del Nopal. Laboratorios Nacionales de Fomento Industrial de México,
Mexico City.
Piña, L., I. 1981. Observaciones sobre la grana y sus
nopales hospederos en el Perú. Cactáceas y Suculentas de
México 26: 10–15.
Portillo, M. L. 1995. Los hospederos de las cochinillas del
carmín (Dactylopius spp.) y algunas consideraciones
sobre su aprovechamiento. In Memorias del 6to
Congreso Nacional y 4to Congreso Internacional Conocimiento y Aprovechamiento del Nopal. Universidad de
Guadalajara, Zapopan, Mexico. Pp. 35–47.
Quirce, S., M. Cuevas, J. M. Olaguibel, and A. I. Tabar.
1994. Occupational asthma and inmmulogical responses induced by inhaled carmine among employees
at a factory making natural dyes. Journal of Allergy and
Clinical Immunology 93: 44–52.
Quispe, I. 1983. Ciclo biológico de la cochinilla del cactus
Dactylopius coccus Costa. En diferentes épocas del año
y en tres pisos altitudinales en Ayacucho. In Memorias
del primer seminario Departamental de Producción y
Fomento de Tuna y Cochinilla. Ayacucho, Peru. Pp. 44–
45.
Ramayo-Ramírez, L., C. Saucedo-Veloz, and S. Lakshminarayana. 1978a. Causas de altas pérdidas en nopal hortaliza (Opuntia inermis Coulter) almacenado por refrigeración y su control. Chapingo Nueva Época 10: 33–
36.
Ramayo-Ramírez, L., C. Saucedo-Veloz, and S. Lakshminarayana. 1978b. Prolongación de la vida de almacenamiento del nopal hortaliza (Opuntia inermis Coulter)
por refrigeración. Chapingo Nueva Época 10: 30–32.
Ramírez, G., and C. Aguilar. 1995. The effect of Opuntia
in lowering serum glucose among NIDDM patients.
A systematic review — preliminary findings. In Proceedings: I Conference of the Professional Association for
Cactus Development. Kingsville, Texas. Pp. 71–78.
Rodríguez, D. 1999. Desarrollo de una bebida pasteurizada a base de nopal. In Memorias. VIII Congreso
Nacional y VI Internacional Conocimiento y Aprovechamiento del Nopal. San Luis Potosí, Mexico. Pp. 75–76.
Rodríguez-Félix, A., and H. Soto-Valdez. 1992. Quality
Rosado, J., and M. Díaz. 1995. Propiedades fisicoquímicas
relacionadas con función gastrointestinal de seis
fuentes de fibra dietética. Revista de Investigación
Clinica 47: 283–289.
Ross, G. N. 1986. The bug in the rug. Natural History 86:
67–71.
Sáenz, C. 1995. Food manufacture and by-products. In
Agro-ecology, Cultivation and Uses of Cactus Pear (G.
Barbera, P. Inglese, and E. Pimienta Barrios, eds.).
FAO Plant Production and Protection Paper 132. FAO,
Rome. Pp. 137–143.
Sáenz, C. 1998. Cladodes: A source of dietary fiber. Journal
of the Professional Association for Cactus Development 2:
117–123.
Sáenz, C., M. Vásquez., S. Trumper, and C. Fluxá. 1992.
Extracción y composición química de mucílago de
tuna (Opuntia ficus indica). In Actas: II Congreso
Internacional de la Tuna y Cochinilla. Santiago, Chile.
Pp. 93–96.
Sáenz, C., E. Sepúlveda, and M. Moreno. 1995a. Estudio
de formulaciones para la obtención de mermelada de
nopal (Opuntia ficus-indica). In Resúmenes: XI Congreso
Nacional de Ciencia y Tecnología de Alimentos. Viña del
Mar, Chile. P. 163.
Sáenz, C., E. Sepúlveda, M. Moreno, D. Granger, and N.
Pak. 1995b. Características funcionales de harina de
nopal (Opuntia ficus-indica) y su utilización en la formulación de galletas. In Actas: VI Congreso Nacional y
IV Congreso Internacional Conocimiento y Aprovechamiento del Nopal, Guadalajara, Mexico. Pp. 24–27.
Sáenz, C., N. Pak, E. Sepúlveda, and M. Lecaros. 1997.
Caracterización de harina de cladodio de nopal. In
Memorias: VII Congreso Nacional y V Internacional
sobre Conocimiento y Aprovechamiento del Nopal.
Monterrey, Mexico. Pp. 302–303.
Saltveit, M. E., Jr., and L. L. Morris. 1990. Overview on
chilling injury of horticultural crops. In Chilling Injury
of Horticultural Crops (C. Y. Wang, ed.). CRC Press,
Boca Raton, Florida. Pp. 3–15.
Nopalitos, Mucilage, Fiber, and Cochineal
233
Saltzman, M. 1992. Identifying dyes in textiles. American
Scientist 80: 474–481.
Schlimme, D.V. Marketing lightly processed fruits and
vegetables. HortScience 30: 15–17.
Sepúlveda, E., C. Sáenz, and M. Moreno. 1995. Obtención
y caracterización de harina de nopal (Opuntia ficusindica). In Actas: VI Congreso Nacional y IV Congreso
Internacional sobre Conocimiento y Aprovechamiento
del Nopal. Guadalajara, Mexico. Pp. 28–31.
Sloan, E. 1994. Top ten trends to watch and work on. Food
Technology 7: 89–100.
Spiller, G. 1992. Definition of dietary fiber. In Dietary Fiber
in Human Nutrition, 2nd ed. (G. A. Spiller, ed.). CRC
Press, Boca Raton, Florida. Pp. 15–18.
Sugimoto, N., Y. Goda, J. Suzuki, M. Kuroyanagi, T.
Yamada, K. Yoshihira, and T. Maitani. 1998. Structure
of minor pigments in cochineal dye. Natural Medicines
52: 135–139.
Tekelemburg, A. 1995. La producción de cochinilla (Dactylopius coccus Costa) en ambientes semicontrolados. In
Memorias VI Congreso Nacional y IV Congreso Internacional Conocimiento y Aprovechamiento del Nopal. Universidad de Guadalajara, Zapopan, Mexico. Pp. 48–55.
Tirado, L. E. 1986. Elaboración de una mermelada a base
de nopal. Bachelor’s Thesis, Universidad Autónoma de
Puebla, Puebla, Mexico.
Trachtenberg, S., and A. M. Mayer. 1981. Composition and
properties of Opuntia ficus-indica mucilage. Phytochemistry 20: 2665–2668.
Trejo, A., G. G. Ortiz., A. M. Puebla, M. D. Huizar, M. R.
Munguía, S. Mejía, and E. Calva. 1995. A purified extract from prickly pear cactus (Opuntia sp.) controls
experimentally induced diabetes in rats. In Actas: VI
Congreso Nacional y IV Congreso Internacional sobre
Conocimiento y Aprovechamiento del Nopal. Guadalajara, Mexico. Pp. 300–305.
Trejo-Gonzalez, A., E. Calva, P. J. Monterrubio, R.
Hernández, L. Sánchez, J. Terrazas, M. A. Ruida, and
B. Cobos. 1991. Antidiabetic properties of prickly pear
cactus. In Proceedings of the 2nd Annual Texas Prickly
Pear Council. Kingsville, Texas. Pp. 75–78.
Trejo, A., G. G. Ortiz., A. M. Puebla, M. D. Huizar, M. R.
Munguía, S. Mejía, and E. Calva. 1995. A purified extract from prickly pear cactus (Opuntia sp.) controls
experimentally induced diabetes in rats. In Actas: VI
Congreso Nacional y IV Congreso Internacional sobre
Conocimiento y Aprovechamiento del Nopal. Guadalajara, Mexico. Pp. 300–305.
234
Sáenz-Hernández, Corrales-García, and Aquino-Pérez
Tütem, E., R. Apak, and K. Sözguen. 1996. The interaction of antitumor-active anthraquinones with biologically important redox couples: I. Spectrophotometric
investigation of the interaction of the carminic acid
and mitoxantrone with the Iron (II, III) and copper (I,
II) redox couples. Journal of Inorganic Biochemistry 61:
79–96.
Vallejos, X. 1999. Elaboración y evaluación de la calidad de
un flan con adición de fibra dietética de nopal. Bachelor’s Thesis, Universidad de Chile, Santiago.
Vargas, F. N. 1988. Biología de la cochinilla del carmín
Dactylopius coccus Costa bajo condiciones de laboratorio en Pampa del Arco (2,750 msnm) Ayacucho.
Bachelor’s Thesis, Universidad Nacional de San
Cristóbal de Huamanga, Ayacucho, Peru.
Velasco, P. H. 1988. Estudio del ciclo biológico de la cochinilla o Grana-cochinilla (Dacylopius coccus Costa).
Bachelor’s Thesis, Universidad Autónoma Chapingo,
Chapingo, Mexico.
Vigueras, A. L., and L. Portillo. 1995. Determinación del
ácido carmínico en dos generaciones de Dactylopius
coccus Costa mediante cultivo hidropónico en nopal.
Nakari 4: 41–48.
Villarreal, P. 1996. Obtención de confitados a base de
cladodios de nopal. Bachelor’s Thesis, Universidad de
Chile. Santiago.
von Elbe, J. H. 1977. Status of colors in dairy products.
Dairy and Ice Cream Field 160: 60–62.
Wang, C. Y. 1990. Alleviation of chilling injury of horticultural crops. In Chilling Injury of Horticultural Crops
(C. Y. Wang, ed.). CRC Press, Boca Raton, Florida.
Pp. 281–302.
Wouters, J. 1990. Dyestuff analysis of scale insects by high
performance liquid chromatography (Homoptera:
Coccoidea). In Proceedings of the Sixth International
Symposium of Scale Insect Studies Part II. Institute of
Applied Zoology Agricultural University, Cracow,
Poland. Pp. 61–70.
Zambrano, M. L., A. D. Hernández, and Y. Gallardo.
1998. Caracterización fisicoquímica del nopal. In Fibra
Dietética (F. Lajolo, and E. Wenzel, eds.). Temas en
Tecnología de Alimentos, Vol. 2. Instituto Politécnico
Nacional, Mexico City. Pp. 29–42.
Zimmerman, H. G., and V. C. Moran. 1991. Biological
control of prickly pear, Opuntia ficus-indica
(Cactaceae), in South Africa. Agriculture, Ecosystems
and Environment 37: 29–36.
CHAPTER
› 14 ‹
I N S E C T PE S TS A N D D I S E A S E S
Helmuth G. Zimmermann and Giovanni Granata
Introduction
Insect Herbivores
Cactus Hosts
Classification of Cactus-Feeding Insects
Host Specificity of Cactus-Feeding Insects
Insect Adaptations to Their Cactus Hosts
Endophagous Herbivores and Survival
External Herbivores
Insect Pests on Cultivated Cacti
Distribution of Pests
Predicting Future Pests
Diseases of Cultivated Cacti
Bacteria
Yeasts
Fungi
Native Fungi on Cacti
Other Diseases
Conclusions and Future Prospects
Literature Cited
Introduction
One of the earliest published records of a cactus-feeding
insect dates back to Alexander von Humboldt in 1812
(Ortega 1991), who described how the cochineal insect
Dactylopius coccus had been cultivated in Mexico and
Central America for many centuries before the arrival of
Columbus. Between 1877 and 1895, several other publications mentioned insects feeding on cacti in the United
States. The most comprehensive work on insects of
Cactaceae, published at the beginning of the last century,
was The Principal Cactus Insects of the United States by
Hunter et al. (1912), which lists 324 species, including
species from Mexico and Central America, 92 of which are
recorded as being injurious to cacti.
Early research into the biological control of alien cactus invaders in countries such as Australia, India, and
South Africa made a significant contribution to the study
of cactus-feeding insects. Indeed, several cacti, including
various species in the genera Cylindropuntia, Harrisia,
Opuntia, and Pereskia from North and South America,
have become serious invaders and have been subjected to
biological control programs (Dodd 1940; Pettey 1948;
Annecke and Moran 1978; Moran and Zimmermann
1984; Julien and Griffiths 1998; Olckers and Hill 1999). As
a result, many papers were published on the taxonomy, bi-
235
ology, and ecology of selected natural enemies used to control these cactus weeds biologically (Blanchard 1922;
Cockerell 1929; Dodd 1940; Pettey 1948; De Lotto 1974;
Moran and Cobby 1979; Robertson 1987; Robertson and
Hoffmann 1989; Hoffmann 1991). Some of these insects are
now important pests of cultivated Opuntia species.
The most significant research contributions on cactusfeeding insects resulted from the comprehensive surveys
made by various entomologists in their search for effective
biocontrol agents (e.g., Mann 1969; Zimmermann et al.
1979). These studies focused primarily on the subfamily
Opuntioideae (Cylindropuntia, Opuntia, Tephrocactus)
with some information also on Pereskia and Harrisia of
subfamilies Pereskioideae and Cactoideae, respectively.
Very little is known of the insects associated with other
genera, particularly those in the Pereskioideae and
Cactoideae, because very few of their species have become
invaders outside of their natural habitats. The only such
species that have become invasive outside the Americas are
Cereus jamacaru, Harrisia martinii, and Pereskia aculeata.
Had the whole of the Cactaceae been studied as comprehensively as the Opuntioideae, the number of recorded
cactus-feeding insect species would certainly be much larger. Also, the cactus-feeding insects of the Caatinga region
of Brazil, as well as those of Paraguay and Bolivia, are undersampled and there are many undescribed species in
these regions (Mann 1969). Only limited surveys have been
undertaken of the subfamily Pereskioideae, mainly in
Argentina (Zimmermann et al. 1979).
Early surveys of cactus pathogens are unknown, most
likely because diseases were not considered for biological
control of cactus weeds at that stage. The first noteworthy
publications on cactus diseases came from North America,
where the emphasis was on commercially cultivated ornamentals and native species (Alcorn et al. 1975; Mitchell
1985). Farr et al. (1989) list 10 fungus species causing disease
symptoms for Opuntia ficus-indica. Most of the important
information on cactus diseases is derived from studies of
pathogens in the Mediterranean countries, where diseases
cause considerable damage to cultivated plantings comprised mainly of O. ficus-indica and its many cultivars.
Many of the diseases are polyphagous and are well known
from other crops. Some, mainly in Mexico, have now
spread from native cactus species to those in cultivation.
Insect Herbivores
Cactus Hosts
Compared to other plant families, relatively few cactusfeeding insects have co-evolved with the Cactaceae (Moran
236
Zimmermann and Granata
1980). This may be attributed to the unique morphology
and physiology of the family. The insects that do feed on
succulent Cactaceae usually have co-evolved with their
hosts and have special features and adaptations that allow
them to survive on these hosts. The main driving force in
the evolution of the Cactaceae was the selection for features
that reduce desiccation. This resulted in morphological
and physiological changes, such as succulence (80–90%
water), Crassulacean acid metabolism (CAM; Chapter 4),
a large diversity of alkaloids, abundance of oxalic acid, loss
of leaves, decreased surface/volume ratios, mucilage, and a
tough cuticle (Benson 1982; Barthlott and Hunt 1993).
Many cactus-feeding insects have overcome these barriers
and have adapted in unique ways to these drastic changes.
The complexity of plants is correlated with the number of insect species associated with them, and the
Cactaceae are for the most part architecturally simple —
generally no leaves, hairs, or trichomes (Lawton and
Schröder 1977; Strong and Levin 1979). These traits, together with the reduced niche space, could have contributed to the reduced but highly host-specific insect
fauna observed in the Cactaceae. This is another reason
why so few phytophagous insects worldwide have been
able to adapt to introduced Cactaceae outside the Americas. In comparison, the evolution of cactus diseases is
rapid, because the biochemical characteristics of cactus
cells adapt well to the various disease agents’ requirements.
Thus, epidemics caused by diseases are able to spread rapidly, causing extensive damage to large commercial cactuspear plantations.
Classification of Cactus-Feeding Insects
In the known cactus-feeding insect community some families, such as the Pyralidae (pyralids) and the Cerambycidae
(long-horned beetles), are over-represented, while other important insect families are lacking (Fig. 14.1). The pyralids
are most numerous with approximately 58 species feeding
on cacti, followed by the cerambycids with 33 species, and
the Curculionidae (weevils) with about 20 species. The family Dactylopiidae (cochineals; Chapter 13) is unique to
Cactaceae (Fig. 14.1) and all of its nine species are specific
to the Opuntioideae, with the exception of Dactylopius confertus, which lives on a Cleistocactus sp. (De Lotto 1974).
The family Cactaceae hosts only one species from the family Diaspididae (scale insects), namely Diaspis (Diplacaspis)
echinocacti; this species is host specific to the family. The
total absence of the large insect families, such as the
Noctuidae (noctuid moths) and Pentatomidae (shield
bugs), is astonishing, as cacti should be particularly attractive to these insects. The absence of specific and naturally
60
North America
South America
number of species
50
40
30
20
10
0
Pyralidae
(pyralids)
Cerambycidae Curculionidae Coreidae
(long-horned
(weevils) (twig wilters)
beetles)
Diptera
(flies)
Dactylopiidae
(cochineals)
All other
taxa
insect families
Figure 14.1. The number of cactus-feeding insects in North and South America out of a total of 165 species. Data
partially from Moran (1980).
occurring cactus fruit flies (Tephritidae) is equally astonishing in view of the large diversity of fruit found within the
Cactaceae. Some of the common fruit flies, which are generalist pests on cultivated fruit worldwide, e.g., Ceratitis capitata, have recently switched to Opuntia fruit. Moran
(1980) showed that, for both North and South America,
more insect species feed on the larger opuntias than on the
smaller, narrow-jointed ones, which is consistent with the
typical species/area relationship of phytophagous insects
(Strong 1979).Fgiure14n1.eahrere:
The differences in the number of insect taxa of North
and South American cactus floras suggest a long period of
isolation among the cactus-feeding faunas. In particular,
very few insect taxa are shared. Second, the large diversity
of insects and mites associated with the northern region
suggests that most cacti evolved in North America. Specifically, 35% more cactus-feeding species occur in North
America than in South America. The small number of curculionid and cerambycid species from South America
(Fig. 14.1) is surprising, but more surveys in South America
probably will not reveal many more new species that might
change this imbalance. The Coreidae (squash bugs) are
represented in North America by two large genera,
Chelinidea and Narnia, which are absent from South
America. Instead, in South America the Coreidae are represented by the small genus Leptoglossus, one of the genera
found in both the Americas. The Lonchaeidae (lonchaeid
flies) are well represented in South America, as opposed to
only two species in North America. Their larvae feed internally in the cladodes of mainly Opuntia species.
A fascinating feature of the family Pyralidae is that the
larvae of all true cactus-feeding members of the family in
South America are reddish, whereas those in North
America are blueish, except Ozamia and Sigelgaita, which
vary in color. These aposematic (warning) colors suggest a
defense strategy against predators, although which poisonous larvae they mimic is not known. All members of
the genera Ozamia and Sigelgaita, as well as the only
species in the genus Noctuella in family Pyraustidae (pearl
moths), are primarily fruit feeders and occasionally destroy
large proportions of fruit (Mann 1969). Ozamia species
occur in both North and South America, whereas the other
two genera are found only in South America. Some of the
Ozamia fruit-feeding species are associated with a wide
host range in both the Opuntioideae and Cactoideae.
Because of their ability to consume fruits, it is surprising
that these insects are not more serious pests on cultivated
Opuntia species.
The dominance of internal cladode feeders (Fig. 14.2)
within the major insect families associated with Cactaceae
may be an indication of the advantages of endophagy. The
succulent internal environment, in addition to the tough,
thick, wax-covered epidermis typical of the Cactaceae, provides excellent protection. About 75% of all cactus-feeding
Insect Pests and Diseases
237
Leaf miner 1%
Free-living sap
suckers 9%
Sedentary sap
suckers 6%
Fruit feeders 6%
External feeders 3%
Internal feeders 75%
Figure 14.2. Guild structure of the 170 cactus-feeding insects and
mites. Some insects fall into more than one category.
insects and mites are internal feeders and only 3% of the
species feed externally (Fig. 14.2). The rest are fruit feeders
and sap suckers. Even though only a few insects have been
collected from the subfamily Pereskioideae (Zimmermann
et al. 1979), which contains the primitive, leaf-bearing cactus species, its insect guild differs clearly from that of the
other two subfamilies. It is characterized by a higher incidence of external feeders and leaf-miners, which may be attributed to the presence of prominent leaves.Fgiure142.neahrere:
Host Specificity of Cactus-Feeding Insects
According to Mann (1969), about 80% of the species of cactus-feeding insects are found in genera of which all members are restricted to cactus hosts, indicating a high degree
of host-plant specialization. On the other hand, within the
Cactaceae, very few cactus-feeding insects are monophagous, i.e., restricted to a single host species, such as
Dactylopius salmianus, which is found only on Opuntia
salmianus. Most cactus-feeding insects are oligophagous,
i.e., restricted to a single genus. A few are restricted to a subfamily. For example, species in the Pyralidae and the
Dactylopiidae are mainly associated with the genus Opuntia
within the Opuntioideae, whereas the Cerambycidae feed
on all three subfamilies.
Distinct insect complexes are associated with the sometimes-invoked genera Platyopuntia and Cylindropuntia of
the Opuntioideae, as defined by Backeberg (1976), which
might be regarded as evidence in favor of retaining these
genera. From western Texas to California, three insects
238
Zimmermann and Granata
species—the moth borers (Pyralidae) Cahela ponderosella
and Alberada parabates, and the cerambycid Coenopaeus
palmeri—are all associated with Cylindropuntia, including
the widely distributed Opuntia imbricata. According to
Mann (1969), all attempts to rear the two pyralids on
Platyopuntia were unsuccessful, whereas the cerambycid
was able to develop on Opuntia species when forced by restrictive cage conditions. Species within the large genus
Narnia also show very clear preferences for either Platyopuntia or Cylindropuntia, and the cochineal, Dactylopius
tomentosus, is associated only with Cylindropuntia. Equally,
under natural conditions Cactoblastis cactorum is only associated with Platyopuntia, although under caged condition it can develop on some Cylindropuntia. This specificity between insects and host plants can have important
implications for cactus taxonomists. It can also be a valuable tool for predicting the future pest status of some of
these insects for the large commercial cactus-pear plantations within the insects’ native ranges.
Recent studies reveal host-adapted biotypes within certain cactus-feeding insect species. Two distinct, hostadapted biotypes exist in the cochineal Dactylopius opuntiae. One of the biotypes attacks only large, tree-like
Opuntia species, such as Opuntia ficus-indica, whereas the
other is restricted to low-growing Opuntia species, such as
the O. stricta types in North America (Githure et al. 1999;
Volchansky et al. 1999). This implies that certain Opuntia
weeds can be controlled biologically, using a host-specific
cochineal biotype, without threatening the commercial
plantations of O. ficus-indica. The existence of host-adapted biotypes within the cochineals also has important implications for the biological control of other Opuntia
weeds. Furthermore, host-adapted biotypes may exist
within the commercially cultivated Dactylopius coccus,
which could have far-reaching consequences for the
cochineal industry. In fact, preliminary research suggests
that carmine cochineal populations may differ in their
host-plant preferences (Robles 1999).
The pyralid moth, Cactoblastis cactorum (Fig. 14.3),
has a very wide host range within the genus Opuntia and has
been recorded as developing on at least 29 hosts (Zimmermann et al. 2000). Many of its hosts within the North
American opuntias constitute new associations and are
being controlled very effectively by C. cactorum. Also, the
pyralid is able to develop on all six native Opuntia species
within their native range in Florida ( Johnson and Stiling
1998). This spells disaster to many native opuntias in all of
mainland North America if the insect arrives there (Zimmermann et al. 2000). The damage caused by C. cactorum
to cultivated Opuntia species in Mexico, should it arrive
tive Opuntia species) has permanently switched to the large
commercial plantations of O. ficus-indica in that country.
This indicates that cactus-feeders have a high degree of
host specificity, which is rare among other introduced crop
plants, as shown in a study of crop plants and their insect
pests in South Africa (Moran 1983).
A
Endophagous Herbivores and Survival
B
Figure 14.3. Larval stage of Cactoblastis cactorum: (A) cross section
of infected cladode of Opuntia ficus-indica; and (B) close-up of the
larvae.
there, could be substantial. In any case, McFadyen (1985)
recognized ten biotypes of C. cactorum, based on larval
differences, host plants, and locality records. Host-adapted
biotypes are likely to be found in several other cactus insects,
including the Coreidae, e.g., Narnia species.Fgiure143n.eahrere:
Insect Adaptations to Their Cactus Hosts
Cactus-feeding insects have adapted to the succulence,
thick cuticle, and other characteristics that are highly developed within the Cactaceae as adaptations to a xerophytic habitat. These characteristics may explain why insects indigenous to other continents have been unable to
adapt successfully to this plant family, members of which
have been introduced into those continents as ornamentals, crops, or invaders. The only exceptions are some
polyphagous mealybugs, aphids, grasshoppers, and other
opportunistic feeders. Opuntia ficus-indica, for instance,
has been in South Africa for at least 260 years without accumulating any indigenous phytophages, although the
time available has certainly been more than adequate and
its distribution is wide enough (Moran 1980). More astonishing, none of the native, cactus-feeding insects living
on the nearly 160 cactus species in Chile (including one na-
Nearly 80% of all insects feeding on opuntias bore within
the succulent plant tissues during the larval stages. This endophagy protects them against predators and parasitoids.
The oviposition habits recognized among the cactus-feeding Lepidoptera are also aimed at reducing predation and
parasitism, and all show close adaptations to their cactus
hosts (Hoffmann and Zimmermann 1989). The most
common method, used by the many species in the genera
Cactoblastis and Melitara, entails the female moth depositing her eggs—one on top of the other—in the form of an
eggstick that superficially resembles a spine. The larvae
hatch simultaneously and enter the cladode collectively
through one entrance hole. By entering the cladode more
rapidly, the larvae can avoid the mucilage secretions that
often repel small larvae (Robertson and Hoffmann 1989).
Single, first-instar larvae of Cactoblastis cactorum are deterred from penetrating cladodes successfully by the secretions; even if they do manage to enter, it takes longer than
when they enter gregariously through a single entrance hole
(Hoffmann and Zimmermann 1989). In other pyralids,
such as Tucumania and members in the Pyraustidae (e.g.,
Megastes, Metapleura, and Mimorista), which deposit their
eggs singly on thorns or directly on the surface of their
hosts, egg mortality is higher than in eggstick-producing
species (Hoffmann and Zimmermann 1989). The larvae of
all the pyralids are endophagous and are well protected
from predators and parasitoids. They are brightly colored in
the later instars when they venture out of the cladodes to
pupate, suggesting a possible aposematic function. Some
members of the Pyralidae are important pests on cultivated Opuntia species (Longo and Rapisarda 1995).
Many Opuntia species grow in warm, semiarid regions
where air and soil temperatures can reach levels of 49ºC
and 65ºC, respectively. For most plants, temperatures over
54ºC are lethal, even if they last only a short period (Nobel
1988). Temperatures inside the cladodes of Opuntia species
are even higher, because lack of daytime stomatal opening
associated with CAM prevents water loss and therefore
cooling of the surface (Chapter 4). A temperature of 59ºC
has been measured inside a cladode when the air temperature was only 43ºC (Went 1982). How the endophagous
pyralid larvae survive these temperatures is not known. In
Insect Pests and Diseases
239
South Africa, C. cactorum larvae have been observed leaving the protection of their cladodes during very hot spells,
possibly to avoid the high internal temperatures (Pettey
1948). Such exposure drastically increases the chances for
predation.
Carminic acid (Chapter 13) acts as a potent feeding deterrent to parasitoids and ants (Eisner et al. 1980). Nonetheless, two species in the Pyralidae, Laetilia coccidivora
from North America and Salambona analamprella from
South America, are uniquely adapted to act as both predators of the cochineals and as phytophages. Their larvae are
free-living and tunnel in cladodes underneath cochineal
colonies. For self-protection, the larvae of L. coccidivora sequester carminic acid from the cochineals on which they
prey (Mann 1969; Eisner et al. 1980) and can severely harm
the commercial rearing of Dactylopius coccus in Mexico
(Tito 1998). In any case, sequestering poisons for self-protection is common in insects that feed on poisonous
plants.
With the exception of two chrysomelid (leaf beetles)
species that feed externally on young cladodes, the larvae
of all cactus-feeding species in the Coleoptera (beetles)—
notably families Curculionidae and Cerambycidae — are
endophagous and thus well protected from parasitoids and
predators. Females deposit their eggs individually on the
plant in scar tissue or in cracks and crevices, which are then
covered with a secretion that hardens to provide protection. The presence of larvae is revealed by frass (debris produced by insects) or gum and/or mucilage exudations.
Some of the major pests on cultivated Opuntia species are
in these two families. The adult cerambycids are all very
cryptic and difficult to detect when resting on the bark of
their cactus hosts.
External Herbivores
Apart from the polyphagous species in the Miridae (mirid
bugs), e.g., Hesperolabops picta, all known cactus-feeding
sap suckers (Fig. 14.2) in the Heteroptera (bugs) belong to
family Coreidae (squash bugs). These are seldom abundant, with the exception of Chelinidea species that can develop pest status on cultivated Opuntia species in Mexico
(Gallegos Vazquez and Mendez Gallegos 2000), causing
yellowing around feeding punctures. Most of these sap
suckers feed gregariously. At the least disturbance, they
move quickly to the protected side of the cladode and with
continued disturbance, they drop to the ground to find
cover. They are vulnerable to predation and parasitism and
have evolved no special protection mechanisms, apart from
their rapid movements and the repugnancy typical of
coreids.
240
Zimmermann and Granata
The most extraordinary insects that have co-evolved
with the Cactaceae are the nine species of the genus
Dactylopius of the Dactylopiidae (cochineals; De Lotto
1974; Chapter 13). They are all sedentary, and the females
are protected by a thick waxy covering and the carmine in
their body fluids that gives the insect its typical red color.
The behavior and life cycles of Dactylopius austrinus, D.
coccus, D. opuntiae, and D. tomentosus have been studied in
detail (Dodd 1940; Pettey 1948; Karny 1972; Moran and
Cobby 1979; Moran et al. 1982; Hosking 1984; Perez
Guerra and Kosztarab 1992; Chapter 13). The insects feed
in an exposed position on the surface of the cladodes and
are vulnerable to excessive rain, which washes them off the
plant. Moran et al. (1987) and Moran and Hoffmann
(1987) highlighted adaptations in the insect to avoid excessive damage caused by rain, such as settling in the rain
shadow of a cladode or near the protection of a spine.
Predators, mainly coccinellid beetles, are well adapted to
prey on cochineal in their countries of origin. Predators
that have not co-evolved with the insects are less effective
due to the deterrent effect of carmine and because of the
waxy covering that provides effective protection to the insect (Morrison 1984).
Dactylopius austrinus, D. ceylonicus, D. opuntiae, and
D. tomentosus have all been used successfully in the biological control of cactus weeds in many countries, and they
are rated as one of the most effective groups of biocontrol
agents (Zimmermann and Moran 1982; Moran and
Zimmermann 1984; Julien and Griffiths 1998). Why these
cochineals are so damaging to their hosts is not known.
Some of the hosts, e.g., Opuntia ficus-indica, can support
large populations of D. coccus without being obviously
affected.
Cactus-feeding insects can apparently detect the CO2
gradient associated with the plant’s CAM activity and use
it to their own advantage. The moth Cactoblastis cactorum
detects small fluctuations in CO2 around the background
atmospheric levels, enabling females to recognize the
healthiest and physiologically most active plants (Stange et
al. 1995). Cactophagous insects probably have other adaptations to the CAM pathway, such as the effect of the daily
acidity fluctuations associated with CAM.
Insect Pests on Cultivated Cacti
Distribution of Pests
Considering the number of recorded cactus-feeding insects
and mites, only a few of these species have become pests on
cultivated Cactaceae, mainly Opuntia species (Longo and
Rapisarda 1995). Little is known of the pests of the other
commercially cultivated genera—Cereus, Hylocereus, Selenicereus, and Stenocereus. By far the largest number of pests
are recorded from Mexico, where Opuntia species are extensively cultivated (Table 14.1). This is not surprising, as
Mexico is home to more than 150 native cactus-feeding insects and also the origin of many cultivated Opuntia
species (Bravo 1978; Scheinvar 1999). Of the total of 122
specialist Opuntia-feeding species listed by Mann (1969)
and Zimmermann et al. (1979), only 23 are general pests of
cultivated Opuntia species in Mexico (Tito 1998; Gallegos
Vazquez and Mendez Gallegos 2000).Tabe14ln1.eahrere:
Very few insect pests have been recorded on cultivated
cacti in countries other than Mexico, including the
Mediterranean countries. The cactus-feeding pests recorded from southern Africa, Australia, Madagascar, and India
(Table 14.1) were deliberately introduced for biological
control of Opuntia weeds (Moran and Zimmermann 1984;
Brutsch and Zimmermann 1995). Except for very polyphagous, opportunistic feeders, such as grasshoppers,
aphids, ants, and mealybugs, no record could be found of
a permanent host-switch onto cultivated Opuntia species
from any of the native phytophagous insects in countries
outside of North and South America. No insect pests have
been recorded from cactus pear in Israel (Y. Mizrahi, personal communication). This is an indication of the taxonomic isolation and the uniqueness of the Cactaceae. Only
recently has the introduced Mediterranean fruit fly,
Ceratitis capitata, been recorded as a pest of fruit of
Opuntia ficus-indica in South Africa. The only hostspecific cactus-feeding insect that has followed the spread
of cactus cultivations around the world is the scale insect
Diaspis echinocacti. Because of the geographical isolation of
the Cactaceae, no insect pest of any significance, apart
from D. echinocacti, has been recorded from Ethiopia,
where cactus-pear plantings cover many thousands of
hectares along with the large areas of naturalized populations (Behailu and Tegegne 1997).
Opuntia species (Chapter 10) and, to a lesser extent,
other species in the genera Hylocereus, Selenicereus, and
Stenocereus (Chapter 11) are widely cultivated in many countries. The insect pests on Opuntia species vary considerably,
depending on the country and the continent in which they
are cultivated (Table 14.1). The pests in these countries will
be divided into three geographic areas, beginning with
North and South America. Countries in these regions, especially in North America, have the highest diversity of insect pests. These are native species that have transferred to
cultivations and differ from North to South America. In
Argentina, Cactoblastis cactorum is by far the most serious
pest (Ochoa de Cornelli et al. 1992), but it is absent from
Brazil (Pernambuco) because of slow dispersal from its native range in Argentina, Uruguay, and Paraguay (G. P. de
Arruda, personal communication). Many major pests are
found in Mexico (Table 14.1), including Anastrepha species
(Tephritidae), Cactophagus (Metamasius) species (Curculionidae), Chelinidea species (Coreidae), Cylindrocoptorus
species (Curculionidae), Dactylopius species (Dactylopiidae), Olycella and Laniifera species (Pyralidae), and Sericothrips species (Thripidae; Gallegos Vazquez and Mendez
Gallegos 2000). Several minor pests are also present.
The second area includes southern Africa, Mauritius,
Australia, India, and some West Indian islands (Table 14.1).
These are countries that have deliberately introduced
Cactoblastis cactorum, Dactylopius opuntiae, and other insect species ( Julien and Griffiths 1998) for the biological
control of various Opuntia weeds (Moran and Zimmermann 1984). In most cases, the control is successful. The
former two insects are now pests on cultivated O. ficusindica and its cultivars, and control measures against them
are necessary for successful cultivation of cactus pear
(Annecke et al. 1976). The host-specific mealybug (Pseudococcidae) Hypogeococcus festerianus and the cerambycid
beetle Alcidion cereicola were introduced into Australia and
South Africa for the biological control of Harrisia martinii
and Cereus jamacaru ( Julien and Griffiths 1998). Both of
these insect species will become pests should these countries decide to develop C. jamacaru as a commercial crop,
as is the case in Israel (Mizrahi and Nerd 1999). Although
the curculionid beetle Cactophagus (Metamasius) spinolae
has been released as a biocontrol agent against O. ficusindica in South Africa and is now well established and
effective at one locality in the Eastern Cape, it has not yet
dispersed to cultivated plantations of O. ficus-indica. This
insect has the potential to become a serious pest on cultivated cactus pear plantations in South Africa.
Mediterranean countries, Ethiopia, the Canary Islands,
Iran, and Iraq have very few pests on cultivated Opuntia
species (Longo and Rapisarda 1995; Table 14.1). First, the
pests were not transferred together with the plants at the
time of introduction from their countries of origin; second,
these countries had no biological control projects against
invading Opuntia species; and third, no native insects
transferred to Opuntia hosts. Some opportunistic feeders
are occasionally found causing temporary damage to plants
in these countries. The cactus scale insect, Diaspis echinocacti, is the only insect that followed the introductions of
Opuntia species throughout the world and is now found
wherever such species are grown. A noncactus pest that has
recently started causing damage to cultivated fruit in
many countries, including South Africa and those in the
Insect Pests and Diseases
241
TA B L E 1 4 . 1
Insect pests on cultivated Opuntia species for various geographical areas
Insect order and species
Pest status
Distribution
Remarks
North and South America
Lepidoptera
Cactoblastis cactorum
Megastes cyclades
(Laniifera cyclades)
Olycella nephelepasa
O. subumbrella
O. junctolineella
Sigelgaita chilensis?
Coleoptera
Cactophagus spinolae
(Metamasius spinolae)
C. fahraei (Metamasius fahraei)
Cylindrocopturus biradiatus
C. ganglbauberi
Moneilema variolare
Hemiptera
Chelinidea tabulata
C. vittiger
Narnia femorata
Homoptera
Dactylopius ceylonicus
D. opuntiae
Diaspis echinocacti
(Diplacaspis echinocacti)
Diptera
Anastrepha sp.
Asphondylia opuntiae
Dasiops bennetti
Thysanoptera
Sericothrips opuntiae
Acari
Tetranychus opuntiae
Unknown
Florida, Mexico?
Severe
Severe
Argentina
Mexico, USA
Accidental arrival in Florida attacking native
species; occurrence in Mexico unconfirmed;
South American origin
Gregarious internal feeder; native transfer
Gregarious internal feeder; native transfer
Sporadic
Sporadic
Sporadic
Uncommon
Mexico
Mexico
Mexico
Chile
Solitary internal feeder; native transfer
"
"
Cladode and fruit feeder; native transfer
Severe
Mexico, USA
Stem borer; native transfer; prefers large species
Sporadic
Sporadic
Sporadic
Sporadic
Mexico
Mexico
Mexico
Mexico
Stem borer; native transfer
Solitary cladode borer; native transfer
"
Solitary stem borer; native transfer
Severe
Sporadic
Sporadic
Mexico, USA
Mexico, USA
Mexico
External sap suckers; native transfer
"
"
Uncommon
Argentina
Severe
Sporadic
Sporadic
Mexico, USA
North America
All countries
Native transfer; taxonomy unclear,
needs verification
External sap suckers; native transfer
Scale insect; wide distribution
Native transfer; subject to biological control
Sporadic
Sporadic
Rare
Mexico
Mexico, USA
Mexico
General fruit pest; non-specific
Fruit feeder; native transfer
Internal cladode feeder; native transfer
Common
Mexico, USA
External sap suckers; discolors cladodes;
native transfer
Common
Mexico, USA
External sap suckers; native transfer
Mediterranean region, is the Mediterranean fruit fly,
Ceratitis capitata (Longo and Rapisarda 1995).
Predicting Future Pests
With increasing globalization and the introduction of cactus pear as a commercial fruit and fodder plant into many
countries, many pests will eventually find their way to new
242
Zimmermann and Granata
countries and continents, with potentially disastrous consequences to local cactus-pear industries. Crawlers of
Dactylopius species can be dispersed over long distances by
the wind (Moran et al. 1982). As increased cultivation provides more targets for the pests, their chances of locating
these targets will improve considerably. Once Dactylopius
opuntiae has reached any of the Mediterranean countries in
TA B L E 1 4 . 1 (continued )
Insect order and species
Pest status
Distribution
Remarks
Southern Africa, East Africa, Mauritius, Australia, India, and some smaller islands
Lepidoptera
Cactoblastis cactorum
Coleoptera
Cactophagus spinolae
(Metamasius spinolae)
Homoptera
Dactylopius opuntiae
Diaspis echinocacti
(Diplacaspis echinocacti)
Diptera
Ceratitis capitata
Severe
Southern Africa,
Australia, Cayman,
Nevis, Montserrat
Introduced for biological control of Opuntia
weeds; origin Argentina via Australia and
South Africa
Negligible
South Africa
Introduced from Mexico for the biological control
of Opuntia ficus-indica; very localized and still
absent from cultivations
Severe
Introduced from Mexico for the biological control
of various Opuntia weeds
Sporadic
Southern Africa,
Eastern Africa,
India, Mauritius,
Madagascar
All countries
New
South Africa
Cosmopolitan fruit fly transferred to Opuntia fruit
Original from the Americas
Mediterranean Countries, Ethiopia, Middle East
Homoptera
Diaspis echinocacti
(Diplacaspis echinocacti)
Pseudococcus spp.
Diptera
Caratitis capitata
Sporadic
All countries
Original from the Americas
Sporadic
All countries
Polyphagous pest and common on ornamental
cacti
Sporadic
Most countries
except Ethiopia
Cosmopolitan fruit fly; transferred to Opuntia fruit
which cactus pear is cultivated, it is likely to spread
effectively throughout the region. Presently, cactus pear
cannot be cultivated in South Africa on a large scale without the interference of this cochineal, due to the insect’s
effective dispersal abilities. Figure 14.4 shows countries into
which Dactylopius opuntiae has been introduced intentionally for the biological control of Opuntia weeds. These
countries now serve as focal points for the further spread
of the insect.Fgiure144.neahrere:
The movement of certain other cactus-feeding insects
for biological control purposes also decreases their distance
from new targets. Some of these insects have the potential
to become serious pests on cultivated opuntias and may also
threaten native Opuntia species. The recent dispersal of
Cactoblastis cactorum within the West Indies and eventually to mainland Florida, either through inadvertent intro-
ductions by the nursery trade (Pemberton 1995) or through
natural dispersal, has serious consequences for both the native Opuntia flora and cultivated plants in the United States
and Mexico (Zimmermann and Perez-Sandi y Cuen 1999).
Figure 14.5 shows the history of the introduction and spread
of C. cactorum from Argentina since 1925. The many interceptions at the main entry points into the southeastern
United States of cactus plants containing endophagous C.
cactorum larvae and belonging to the cactus nursery trade
demonstrates how easily the insect’s entry into the country
might pass unobserved (Pemberton 1995). A lively cactus
trade also exists between Europe and many American
countries, and the arrival of the insect into Mediterranean
countries is therefore almost inevitable.Fgiure145n.eahrere:
In the state of Pernambuco, Brazil, cultivated Opuntia
species are important as a source of fodder, and about
Insect Pests and Diseases
243
Santa Cruz Island
(California), 1951
India, 1926
Approximate
natural distribution
Indonesia, 1935
Hawaii, 1949
Sri Lanka, 1924
Mauritius, 1928
Madagascar, 1923
South Africa, 1938
Australia, 1921
Figure 14.4. Countries where Dactylopius opuntiae has been intentionally released for the biological control of Opuntia weeds.
400,000 ha are under cultivation (Barbera 1995). This can
provide an easy target for both C. cactorum from the south
and Dactylopius opuntiae from the north. Increased cultivation of cactus pear between Paraguay and Pernambuco
will eventually form a host chain that might allow C. cactorum to spread naturally to these areas. The Andes form
a natural barrier between the Opuntia species infested with
C. cactorum in Argentina and the pest-free cultivations in
Chile but, with increasing cultivation of O. ficus-indica in
some Andean valleys in Argentina, these distances have decreased considerably. Within Africa, both C. cactorum and
D. opuntiae may disperse northward from Zimbabwe via
Kenya to Ethiopia because the cultivation of O. ficus-indica
in the arid zones between these two countries is being
encouraged.
Little is known of the dispersal behavior of the other
major cactus pear pests in Mexico. However, with the increasing cactus trade and the large number of amateur cactus collectors moving cacti between countries and continents, the inadvertent dispersal of new insect pests is
inevitable.
Diseases of Cultivated Cacti
The most important cactus pear diseases can be grouped
according to their pathogenic agents (Table 14.2). The biotic diseases are caused by bacteria, yeasts, fungi, phytoplasmas, viruses, and some not-so-well-defined agents
called phytoplasma/virus-like organisms. Some diseases
and disease-like symptoms on cactus pear can also be
caused by abiotic conditions, e.g., environmental stress
(such as hailstorms), genetic anomalies, incorrect pesticide
244
Zimmermann and Granata
application, and physiological disorders. Biotic diseases of
cactus pear are present in all the growing areas. They are
influenced by the presence of the pathogen as well as climatic conditions. For example, bacterial diseases are more
prevalent in Chile, Argentina, Mexico, and Italy than in
Peru and North Africa because the elevated temperatures
and low humidity in the latter two regions do not favor
this type of disease. Although fungal diseases are also influenced by environmental conditions, some have not
been reported in certain countries, probably because the
pathogen is not present in that region. For example,
Alternaria golden spot is present and causes severe damage
in Mexico, Italy, and South Africa but not in other cactuspear growing countries; Aecidium spp. provokes major disease only in Peru; Cercospora spp. is prevalent in Peru and
Bolivia; Phoma sorghina causes damage only in Argentina;
and Dothiorella ribis, the causal agent of a cactus-pear gum
cancer, has been found only on the small island of Linosa
(Italy). Few systematic studies have focused on cactus pear
viral diseases, even though various reports exist (Chessin
1965).Tabe14l2.neahrere:
The evolution of cactus diseases is very rapid, as the
biochemical characteristics of the cell juices adapt well to
the various biotic agents’ requirements. This extremely important fact should highlight the urgency of preventing
diffusion of diseases that can rapidly become epidemic.
Strict control must be performed on propagation material. Prevention is the best approach to guarantee successful
cultivation of cacti, and propagation material and fruit
should not be imported from areas where specific diseases
are present.
Unintentional:
Cuba, 1991, Haiti, Dominican
Republic, Puerto Rico,
Bahamas, Florida, 1989,
Virgin Islands
Intentional:
Antigua, 1960,
Montserrat, 1960,
Nevis, 1957
Intentional:
Pakistan?
1971
Intentional:
Ascension
Island, 1973
Intentional:
Hawaii, 1950
Intentional:
New Caledonia,
1933
Intentional:
Kenya? 1971
Approximate natural
distribution
Intentional:
Mauritius, 1950
Intentional:
St. Helena, 1971
Intentional:
Southern Africa,
1933
Intentional:
Australia,
1926
Figure 14.5. Intentional and unintentional introduction of Cactoblastis cactorum. Establishment in Kenya and Pakistan is not confirmed.
Bacteria
Cactus pear tissues have a relatively high water content and
therefore infections, especially bacterial infections, rapidly turn into rot (Table 14.2). Saprophitic colonization occurs, which makes it difficult to isolate the pathogenic
agent. Bacteria are prokaryotes. About a hundred species
lead to plant and animal diseases (Bradbury 1970;
Buchanan and Gibbons 1974). The most common pathogenic genera of bacteria are Agrobacterium, Corynebacterium, Erwinia, Pseudonomas, and Xanthomonas (Skerman
et al. 1980; Krieg 1984).
Bacterial agents do not possess penetrating mechanisms and can only access plants through wounds. Infection requires specific weather conditions characterized
by low temperatures and elevated atmospheric humidity
plus the presence of a film of water on the plant tissues.
Consequently, bacterial diseases are more widespread in
moist regions or during wetter times of the year. Bacteriainduced symptoms are caused by cell death (necrosis) and
sometimes abnormal growth (tumors) of the infected organs due to hyperplasia (abnormal cell multiplication) or
hypertrophy (abnormal cell size), and they generally result
in soft rots in the Cactaceae (Lelliott and Stead 1987).
Bacterial spot has been reported from India, Italy, and
certain other cactus-pear growing countries (Argentina,
Chile, and Mexico). It is the most severe cactus pear dis-
TA B L E 1 4 . 2
Biotic pathogenic agents of cactus pear diseases
Bacterial Diseases
Bacterial spot (Erwinia carotovora, E. cacticida)
Crown gall (Agrobacterium tumefaciens)
Yeast Diseases
Soft rot (Candida boidimi)
Fungal Diseases
Armillaria rot (Armillaria mellea)
Alternaria golden spot (Alternaria alternata)
Foot rot (Phytophthora cactorum, P. nicotianae)
Gray mold (Botrytis cinerea)
Other fungal agents: Aecidium sp., Cercospora sp., Colletotricum
sp., Cytospora sp., Hansfordia sp., Fusarium oxysporum,
Gleosporium herbarum, Mycospherella sp., Phoma sorghina,
Phyllosticta opuntiae, Phyllosticta concava, Pleospora herbarum,
Sclerotinia sclerotiorum
Phytoplasmalike and Viruslike Diseases
Cladode enlargement
Flower proliferation
Insect Pests and Diseases
245
ease in areas where winter or spring frosts and hailstorms
wound cladodes or fruits. High summer temperatures arrest the infection, but the bacterium (Erwina species) becomes active as soon as cooler, moist conditions prevail
(Cortés and Fucikovsky 1986; Alcorn and Orum 1988;
Fucikovoky 1990; Granata and Varvaro 1990; Varvaro et al.
1993). The main pathogenic organism of bacterial spot is
Erwinia carotovora (Table 14.2), which is rod-shaped,
gram-negative, motile by perytrichois flagella, and aerobic.
This bacterium grows at temperatures up to 36°C, but the
optimal temperature is 10 to 15°C. In addition to cactus
pear, it can also infect other species of Opuntia. Symptoms
appear on the cladodes in the spring and consist of translucent spots that coalesce, darken, and eventually turn black.
Parenchymatous tissues under the integument are initially water-soaked and then turn brown to nearly black. The
superficial infected tissues dry, develop into a scab, and are
frequently cracked (Fig. 14.6A). To control the disease, the
infected cladodes must be removed. They must not be
buried in the plantation area and burning is recommended. Two copper-based fungicide applications are recommended in the winter-spring where the disease is widespread and after hailstorms or other events that cause plant
wounds. In Mexico the black soft rot on cactus pear is
caused by Erwinia cacticida and coffee rot is caused by Erwinia carotovora subsp. atrospetica (Hernandez et al. 1997)
Crown gall is another bacterial disease that occurs on
cactus pear in Mexico under the name “Agalla del Nopal”
(Gutiérrez 1992) and is present worldwide. The pathogenic agent is Agrobacterium tumefaciens (Table 14.2), which
affects diverse species of plants. It lives in the soil as a
saprophyte and colonizes the upper roots and the crown of
a plant. Symptoms consist of tumoral masses at the base of
the stem. The tumors can become 10 cm in diameter, are
dark in color, and are cracked when mature. A dark
amber exudate, which oozes from the stem near the
tumor, can be mistaken for fungal infections. In reality, the
tumor does not substantially damage the plant, but it
should still be removed and destroyed. Copper-solution
treatments can control the disease, and wound closure
using gum mastic is recommended.
Yeasts
Yeasts are larger than bacteria and possess some morphologic and structural features placing them between bacteria and other fungi (Lodder 1974; Kreger 1984). They are
single-celled organisms and, unlike bacteria, are eukaryotes
(possess a nucleus) and can develop under both aerobic
and anaerobic conditions. Yeasts respire and promote fermentation utilizing monosaccharide sugars, i.e., sugars
246
Zimmermann and Granata
with six carbon atoms that often derive from disaccharides
(e.g., sucrose) cleaved by enzymes. Yeasts attack plants that
have been debilitated by fungal or bacterial infection; they
are more destructive than bacteria, as fermentation eventually kills the entire infected organ.
The yeast disease cladode soft rot (Fig. 14.6B) is frequently associated with bacterial spot (Phaff et al. 1978,
1985; Starmer et al. 1978; Granata and Varvaro 1990). The
pathogenic agent, Candida boidimi (Table 14.2), is most active at temperatures between 20 and 35°C. The initial
symptoms are black areas caused by internal tissue infection on the cladodes and fruits, followed by internal tissue
rot, after which the plant becomes a deliquescent mass.
The entire cladode surface blackens, but the external tissue
does not rot and thus the cladodes resemble a limp handbag containing a foul-smelling liquid. Control is most
effective when the infected cladodes are removed and destroyed followed by a 1% Bordeaux mixture treatment.
Treatments used against bacterial diseases, such as bacterial spot, are also efficacious.
Fungi
Fungi cause both plant and animal diseases. To date about
100,000 species of fungi have been studied and over 800
possess more or less virulent plant pathogenic action.
Fungal infections cause physiological alterations in host
plant transpiration, respiration, and nutrition and lead to
damage ranging from stunted growth and production to
death of the whole plant, or part thereof. Indeed, fungi are
the most common pathogenic agents in the plant world,
including the Cactaceae. Characterized by a vegetative
stage made up of mycelium (hyphae) that ensures fungal
growth, fungi have a parasitic relationship with the host
plant. Hyphae produce spores by agamic processeses or
conidia. Spores and conidia are propagation forms that enable fungal diffusion in the environment. When they come
into contact with receptive hosts under suitable environmental conditions, they germinate and produce hyphae,
some of which penetrate intact surfaces or access the plant
through natural openings, e.g., stomata or wounds. As previously noted, cacti have few natural openings and a tough
waxy epidermis, thus pathogenic agents rarely penetrate
the skin and cell wall; however, they are vulnerable to pathogenic attack through wounds. Fungal diseases can be diagnosed by typical symptoms and microscopic examination of the structure and reproductive bodies of the fungus
on the colonized organs or in artificial media (Agrios 1978).
Armillaria root and stem rot (Table 14.2) is a slowly
progressing disease that leads to the death of a plant. It is
caused by Armillaria mellea, a basidiomycetous fungus that
A
B
Figure 14.6. (A) Cladode of Opuntia ficus-indica with symptoms of bacterial spot and (B) dark areas of cladode caused by the yeast disease, soft rot.
colonizes the shoot and main roots of the plant and produces white mycelium and cordons of hyphae that spread
from the infected tissues and contaminate neighboring
plants. The presence of hyaline, elliptical, and smooth basidiospores produced by carpophores are characteristic of
infections caused by this disease. Infected plants suffer a
decline in tissue turgor and chlorosis resulting from
phloem disorganization and altered water and mineral nutrition. Fruits borne by the infected plant are small and do
not ripen. The infected tissue at the stem base eventually
rots and releases a viscous exudate. A white mycelium
emitting a characteristic fungus smell is found under the
bark. The contaminated portion of the shoot has a brightred border (Redfern 1968, 1973; Raabe 1979; Mangano di
San Lio and Tirrò 1983). This fungus is ubiquitous,
polyphagous, and often remains in the soil in residues from
previous cultivations, thus inevitably infecting new crops.
It is advisable not to cultivate Armillaria-infected soil for
2 or 3 years and to remove sources of inoculum (roots of
previous cultures). Effective chemical control of the disease
is not yet available.
Alternaria golden spot (Fig. 14.7A) has been reported
in Mexico under the name of “Mancha de Oro” and is also
present in Italy and South Africa. It is caused by the fungus Alternaria alternata (Table 14.2) that generally penetrates the plant near the spines of cladodes. A slightly raised
round spot appears close to the site of penetration; the underlying tissue then changes from dark to light green and
the spot becomes golden. Spots can also appear on the
fruits. Spraying with a copper- or Captan-based fungicide
can be an effective control for this disease. Ideally, treatment should be applied to potentially vulnerable plants
prior to the onset of the symptoms.Fgiure147.neahrere:
Phytophthora cactorum (Fig. 14.7B) and P. nicotianae are
the pathogenic agents of foot rot (Table 14.2) and are
found in cultivations of cactus pear and other Cactaceae
(Cacciola and Magnano di San Lio 1988). These fungi live
in the soil and usually attack the plant shoot and roots, es-
Insect Pests and Diseases
247
pecially in irrigated areas where surface water is present.
Gum begins to ooze from the stem base of infected plants
at the end of the spring, exuding the greatest amounts during the summer. Diseased innermost tissues rot and turn
reddish. The rot may involve the entire stem circumference
and kill the plant. Infected plants become chlorotic,
growth slows, and cladodes wilt, causing the plant to fall
over. Clay soils where water stagnates are prone to infection
with Phytophthora species. Thus, cactus pear should be cultivated in sandy soils, and applications of specific fungicides are recommended wherever this disease prevails.
Gray mold of cactus pear fruits is caused by the fungus
Botrytis cinerea (Fig. 14.8A). It can start anywhere on the
plant, but it generally begins in wounds caused when removing fruits from the cladodes. The infected areas are usually gray and round. When peeled, the underlying fruit tissues appear decayed and soft. The infection spreads in hot
humid areas, with the entire fruit becoming a soft, decaying mass. Typical green or blue colonies of Penicillium are
often observed on the diseased fruit surface (Fig. 14.8A).
Gray mold is difficult to control on cactus pear fruits, as the
spines and glochids on fruits cause wounds that promote
infection during harvesting and processing.Fgiure148.neahrere:
Cactus pear is host to many other fungi that can cause
damage under particular environmental and host conditions. Aecidium species induces the disease called “Roya” in
Peru, leading to small chlorotic spots on the cladodes and
fruits in the early summer; the spots enlarge and give rise
to pustules with orange-colored centers that deform the
fruit. Infected fruit do not ripen fully. Cercospora spp. induce circular (1.0–1.5 cm diameter) necrotic wounds on
the cladodes (Fig. 14.8B) and fruits and is the most serious
cactus disease in Peru and Bolivia. In Mexico Colletotricum
spp. cause dark brown spots with reddish tints that generally start at the edges and then extend over the entire cladode or fruit. Cytospora spp., Gleosporium spp., Mycospherella
spp., Phoma sorghina (Fig. 14.8C), and Pleospora herbarum
also produce necrotic spots on cactus pear cladodes and are
found in various countries. Fusarium oxisporum f.s. opuntiarum, the causal agent of “Fusarium wilt,” affects the vascular tissues and causes wilting of cladodes and fruit, leading to a reddening of infected tissues. Phyllostica concava
and Phyllostica opuntiae occur in Mediterranean areas and
Mexico, respectively, and produce small, round, rust-colored “scabies” for cactus pear; the fungal fructifications appear as black spots. A Phyllostica species is also causing
damage to Opuntia ficus-indica cultivated in Argentina
(Wright 1997). Sclerotinia sclerotiorum causes a cottony rot
on cladodes of cultivated cactus pear in Chile and on native Opuntia species in Argentina. The cuticle softens, the
248
Zimmermann and Granata
contaminated tissue then darkens, flakes off, and is covered
with white wool; soon numerous black sclerotia appear
from the decayed tissues. Hansfordia species are other fungi
found in Mexico that cause black spots (Hernandez 1992).
Native Fungi on Cacti
Very few surveys have been made of native cactus diseases
within North and South America. South African pathologists have surveyed for potential pathogens to be used for
the biological control of jointed cactus, Opuntia aurantiaca (Moran and Annecke 1979; Mildenhall et al. 1987). Some
of these diseases may switch to cultivated Opuntia species
once the areas under cultivation in the Americas and elsewhere increases. The most prominent diseases within native
Opuntia species are Aureobasidium pullulans, Fusarium
proliferatum, and Microdochium lunatum (Mildenhall et al.
1987). The latter disease already occurs widely on native and
cultivated Opuntia species in Argentina, the southwestern
United States, South Africa, Australia (Dodd 1940), and the
Canary Islands (Von Arx 1984), and on Opuntia ficus-indica
in Spain (Wollenweber and Reinking 1935). This disease
was studied in South Africa as a potential biocontrol agent
against O. aurantiaca (Witbooi 1991). The disease forms circular necrotic spots that eventually collapse and fall out,
leaving a clear circular opening. Under certain conditions,
the fungus can cause rotting of entire cladodes.
Other Diseases
A disease leading to cladode enlargement has been reported in Mexico under the name of “Engrosamiento excesivo de
cladodios” and can occasionally develop into a serious problem for cactus pear cultivation in that country (PimientaBarrios 1974; Gutierrez 1992); the causal agent is unknown
(Hernandez 1992). The characteristic symptoms of this disease are stunted plant growth, followed by cladode swelling
and gradual loss of the plant’s green color. Flower production is reduced and flowers generally form on the flat part
of the cladodes, whereas on healthy cladodes flowers form
on the upper edge. Fruit production is poor in terms of
both number and weight. Cladodes from vigorous, healthy
plants should be chosen for planting. During the first
years, any plants manifesting the symptoms of cladode enlargement in new cactus-pear plantations should be removed and replaced with healthy ones. In old plantations,
infected plants must be eliminated and destroyed.
Flower proliferation is a cactus-pear disorder that has
been encountered only in Mexico, on a limited number of
natural and cultivated varieties. The disease is spreading in
Mexico and could pose serious damage to cactus-pear cultivations if not controlled by selection. This disease is char-
A
B
Figure 14.7. (A) Cladodes and fruits infected with Alternaria golden spot and (B) foot rot caused by Phytophora cactorum.
B
A
C
Figure 14.8. (A) Gray mold caused by Botrytis cinerea on fruit, with secondary fungus Penicillium sp.; (B) cladode infected by Cercospora; and
(C) necrotic spots caused by Phoma sorghina.
acterized by excessive flower production all over the cladode surface, early detachment of flowers (receptacles), and
premature spine abscission. The young cladodes are deformed, and new fruits may differentiate from the upper
part of fruits. Finally, some phytoplasma and virus-like cactus pear diseases can be transmitted by grafting but the
pathogenic agents are not known. The microorganisms involved cause alterations in the phloem and consequent
stunted plant growth, yellowing, and low productivity.
Conclusions and Future Prospects
The cactus-feeding insects associated with subfamily
Opuntioideae are well documented, but those associated
with the other subfamilies, Cactoideae and Pereskioideae,
are little known and need further study, particularly in
some South American countries. The known insect guild
is characterized by a high degree of specificity and unique
adaptations to the Cactaceae. The insect families Pyralidae, Cerambycidae, and Curculionidae dominate the
guild, and all their cactus-feeding species are endophagous. Of the nine species in the Dactylopiidae, which
are all associated with Cactaceae, four species have become
well known as highly successful biological control agents
against invasive Opuntia species and a fifth one, Dac-
250
Zimmermann and Granata
tylopius coccus, is reared commercially as a source of the red
dye, carmine.
With the increasing importance of commercial cactus
plantings and larger areas under cultivation, some of the
key pests will find their way to new targets. Knowing and
understanding the potential threats may help to prevent
unwanted arrivals. The dispersal of Cactoblastis cactorum
and its subsequent invasion of mainland America is a case
study. The chances of insects from non-cactus hosts adapting to become pests on cactus cultivations are small, and
the recruitment of new pests in the major cactus growing
countries will depend mainly on natural dispersal and
human intervention of existing cactus pests. This applies
particularly to cactus-pear cultivation in countries outside
of the Americas. The recruitment of new pests from the
large source of cactus-feeding insects and diseases native to
the Americas is also possible in time.
The internal feeding habits of most cactus-feeding insects will make conventional control methods, e.g., insecticidal control, very difficult. Biological control has been attempted against only one cactus pest, Diaspis echinocacti,
but biological control projects are now being considered
against C. cactorum and other species. Research will continue on the use of host-specific, cactus-feeding insects for
the biological control of cactus invaders outside their natural distribution. The selection of host-adapted biotypes of
Dactylopius tomentosus for the biological control of Opuntia
rosea is presently being examined in South Africa, which is
expected to provide further useful information on the existence of host-adapted biotypes. Studies on biological control of cactus weeds have provided valuable information on
many of the key cactus-feeding insects (Moran and
Zimmermann 1984). Likewise, research on C. cactorum by
South African and Australian entomologists will provide
useful information when formulating a strategy to prevent
its invasion or, eventually, its control on the North
American continent (Zimmermann et al. 2000).
Cactus diseases are not extensively studied, as revealed
by the relatively few publications. Most research on cactus
diseases has emerged from Mediterranean countries, e.g.,
Italy. Even less research has been conducted on the natural diseases found on native cactus species in the Americas.
With increased cultivation of cactus pear in America and
elsewhere, some of the less host-specific latent diseases are
expected to gain in importance when switching to commercial plantations.
Acknowledgments
Thanks are expressed to the following persons for providing information on cactus pests and selected literature:
Mayra Perez-Sandi y Cuen, Antonio Bustamante, Yosef
Mizrahi, Carmen Saenz, Carlos Nostas, and Enrique Lobo.
Hildegard Klein provided valuable criticism and edited the
draft manuscript.
Literature Cited
Agrios, G. N. 1978. Plant Pathology, 2nd ed. Academic
Press, New York.
Alcorn, S. M., R. L. Gilbertson, and M. R. Nelson. 1975.
Some diseases of cacti in Arizona. Progressive Agriculture in Arizona 17: 3–6.
Alcorn, S. M., and T. V. Orum. 1988. Rejection of the
names Erwinia carnegieana 1942 and Pectobacterium
carnegana (Standring 1942) Brenner, Teigerwait, Miklos
and Fanning 1973. International Journal of Systematic
Bacteriology 38: 132–134.
Annecke, D. P., W. A. Burger, and H. Coetzee. 1976. Pest
status of Cactoblastis cactorum (Berg) (Lepidoptera:
Phycitidae) and Dactylopius opuntiae (Cockerell)
(Coccoidea: Dactylopiidae) in spineless Opuntia plantations in South Africa. Journal of the Entomological
Society of Southern Africa 39: 111–116.
Annecke, D. P., and V. C. Moran. 1978. Critical reviews of
biological pest control in South Africa. 2. The prickly
pear, Opuntia ficus-indica (L) Miller. Journal of the
Entomological Society of Southern Africa 41: 161–188.
Backeberg, C. 1976. Das Kakteenlexicon. Gustav Fischer
Verlag, Stuttgart.
Barbera, G. 1995. History, economic and agro-ecological
importance. In Agro-ecology, Cultivation and Uses of
Cactus Pear (G. Barbera, P. Inglese, and E. PimientaBarrios, eds.). FAO Plant Production and Protection
Paper 132. FAO, Rome. Pp. 1–11.
Barthlott, W., and D. R. Hunt 1993. The Cactaceae. In The
Families and Genera of Vascular Plants. (K. Kubitzki,
ed.). Springer-Verlag, Berlin. Pp. 161–197.
Behailu, M., and F. Tegegne (eds.). 1997. Proceedings of the
International Workshop on Opuntia in Ethiopia: State of
Knowledge in Opuntia Research. Mekelle University
College, Mekelle, Ethiopia.
Benson, L. 1982. The Cacti of the United States and Canada.
Stanford University Press, Stanford, California.
Blanchard, E. E. 1922. Apuntes sobre Zophodia analamprella Dyar y otros Lepidopteros que viven sobre Cactaceae en la Argentina. Physis 6: 119–123.
Bradbury J. F. 1970. Isolation and preliminary study of
bacteria from plants. Reviews of Plant Pathology 49:
213–218.
Bravo, H. H. 1978. Las Cactaceas de Mexico. Universidad
Nacional Autónoma de Mexico, Mexico City.
Brutsch, M. O., and H. G. Zimmermann. 1995. Control
and utilization of wild opuntias. In Agro-ecology, Cultivation and Uses of Cactus Pear (G. Barbera, P. Inglese,
and E. Pimienta-Barrios, eds.). FAO Plant Production
and Protection Paper 132. FAO, Rome. Pp. 155–166.
Buchanan, R. E., and N. E. Gibbons. 1974. Bergey’s
Manual of Determinative Bacteriology, 8th ed. Williams
& Wilkins, Baltimore, Maryland.
Cacciola, S. O., and G. Magnano di San Lio. 1988. Foot
rot of prickly pear cactus caused by Phitophthora nicotianae. Plant Disease 72: 793–796.
Chessin, M. 1965. Wild plant hosts of a cactus virus.
Phytopathology 55: 933.
Cockerell, T. D. A. 1929. Biological control of prickly-pear.
Science 69: 328–329.
Cortés, L., and L. Fucikovsky. 1986. Pudrición blanda de
las pencas del nopal (Opuntia sp.). Memorias XIII Con-
Insect Pests and Diseases
251
gresso Nacional de Fitopatologia. Tuxtla Gutiérrez,
Mexico. P. 194.
De Arruda, G. P., F. G. P. Arruda, and J. F. Warumby. 1999.
Introducción y utilización de las Cactaceas Nopalea
cochenilifera (L.) y Opuntia ficus-indica en Brasil. Proceedings of the VIII Congreso Nacional y VI Internacional
sobre el Conocimiento y Aprovechamiento de Nopal. San
Luis Potosí, Mexico. Pp. 101–102.
De Lotto, G. 1974. On the status and identity of the
cochineal insects (Homoptera: Coccoidea: Dactylopiidae). Journal of the Entomological Society of Southern
Africa 37: 167–193.
Dodd, A. P. 1940. The biological campaign against prickly-pear. Commonwealth Prickly Pear Board Bulletin.
Brisbane, Australia. Pp. 1–177.
Eisner, T., S. Nowicki, M. Goetz, and J. Meinwald. 1980.
Red cochineal dye (carminic acid): Its role in nature.
Science 208: 1039–1042.
Farr, D. F., G. F. Bills, G. P. Chamuris, and A. Y Rossman.
1989. Fungi on Plants and Plant Products in the United
States. APS Press, St. Paul, Minnesota.
Fucikovsky, L. 1990. Associación de insectos con la prudicíon blanda de cladodios. Resumenes IV Reuníon
Nacional sobre el Conocimiento y Aprovechamiento del
Nopal. Zacatecas, Mexico. P. 38.
Gallegos Vasquez, C., and S. de J. Mendez Gallegos. 2000.
La tuna: criterios y técnicas para su producción comercial. Fundación Produce Zacatecas. Universidad
Autónoma Chapingo, Chapingo, Mexico. Pp. 95–116.
Githure, C. W., H. G. Zimmermann, and J. H. Hoffmann. 1999. Host specificity of biotypes of Dactylopius
opuntiae (Cockerell) (Hemiptera: Dactylopiidae):
Prospects for biological control of Opuntia stricta
(Haworth) Haworth (Cactaceae) in Africa. African
Entomology 7: 43–48.
Hoffmann, J. H. (ed.). 1991. Biological control of weeds in
South Africa. Agriculture, Ecosystems and Environment
37: 1–255.
Hoffmann, J. H., and H. G. Zimmermann. 1989. Ovipositional and feeding habits in cactophagous pyralids:
Prediction for biological control of cactus weeds.
Proceedings of the VII Symposium on Biological Control
of Weeds. MAF, Rome. Pp. 395–399.
Hosking, R. J. 1984. The effect of temperature on the population growth potential of Dactylopius austrinus De
Lotto (Homoptera: Dactylopiidae) on Opuntia aurantiaca. Journal of the Australian Entomological Society 23:
133–159.
Hunter, W. D., F. C. Pratt, and J. D. Mitchell. 1912. The
principal cactus insects of the United States. United
States Department of Agriculture, Entomological Bulletin
113: 1–71.
Karny, M. 1972. Comparitive studies on three Dactylopius
species (Homoptera: Dactylopiidae) attacking introduced opuntias in South Africa. Department of
Agricultural Technical Services, Entomology Memoir 26:
1–19.
Kreger, N., and W. R. van Rij. 1984. The Yeasts. A Taxonomic Study, 3rd ed. Elsevier, Amsterdam.
Krieg, N. E. 1984. Bergey’s Manual of Systematic Bacteriology, 8th ed., Vol. 1. Williams & Wilkins, Baltimore,
Maryland.
Johnson, D. M., and P. D. Stiling. 1998. Distribution and
dispersal of Cactoblastis cactorum (Lepidoptera:
Pyralidae), an exotic Opuntia-feeding moth, in Florida.
Florida Entomologist 81: 12–22.
Granata, G., and L. Varvaro. 1990. Bacterial spots and necrosis caused by yeasts on prickly pear cactus in Sicily.
Proceedings of the 8th Congress of the Mediterranean
Phytopathological Union, Agadir, Morocco. Pp. 467–468.
Julien, M. H., and M. W. Griffiths (eds.). 1998. Biological
Control of Weeds: A World Catalogue of Agents and Their
Target Weeds, 4th ed. CABI Publishing, Wallingford,
United Kingdom.
Gutiérrez, L. H. 1992. Plagas y Enfermedades del Nopal en
México. Universidad Autónoma Chapingo, Chapingo,
Mexico.
Lawton, J. H., and D. Schröder. 1977. Effects of plant
type, size of geographical range and taxonomic isolation on number of insect species associated with
plants. Nature 265: 137–140.
Hernandez, J. C. 1992. Plagas, enfermedades y otros factores que afectan al nopal. Proceedings of the V National
and III International Congress on the Understanding and
Utilization of Cactus Pear. Chapingo, Mexico. P. 89.
Hernandez, J. C, L. Fucikovsky, and A. R. Dionicio. 1997.
Erwinias en la pudrición del nopal (Opuntia spp.) en
252
differentes zonas nopaleras de Mexico. Proceedings of the
VII National and V International Congress on the Understanding and Utilization of Cactus Pear. Monterrey,
Mexico. Pp. 193–194.
Zimmermann and Granata
Lelliot, R. A., and D. E. Stead, 1987. Methods for the
Diagnosis of Bacterial Diseases of Plants. Blackwell,
Oxford.
Lodder, J. (ed.). 1974. The Yeasts: A Taxonomic Study.
North-Holland, Amsterdam.
Longo, S., and C. Rapisarda. 1995. Pests of cactus pear. In
Agro-ecology, Cultivation and Uses of Cactus Pear. FAO
Plant Production and Protection Paper 132. FAO,
Rome. Pp. 100–108.
Magnano di San Lio, G., and A. Tirrò. 1973. Una moria del
ficodindia causata da Armillaria mellea. Informatore
Fitopatologico 1: 47–50.
Mann, J. 1969 Cactus-Feeding Insects and Mites. Smithsonian Institution Bulletin 256, Washington, D.C.
McFadyen, R. E. 1985. Larval characteristics of Cactoblastis
spp. (Lepidoptera: Pyralidae) and the selection of
species for biological control of prickly pears (Opuntia
spp.). Bulletin of Entomological Research 75: 159–168.
cochineal insect Dactylopius austrinus (Homoptera:
Coccoidea: Dactylopiidae). Ecological Entomology 7:
409–419.
Moran, V. C., and H. G. Zimmermann. 1984. The biological control of cactus weeds: Achievements and
prospects. Biocontrol News and Information 5: 297–320.
Morrison, J. F. 1984. Protection from beetle-predation in
cochineal insects (Dactylopiidae: Homoptera). Master’s
Thesis, Rhodes University, Grahamstown, South
Africa.
Nobel, P. S. 1988. Environmental Biology of Agaves and
Cacti. Cambridge University Press, New York.
Mildenhall, J. P., S. M. Alcorn, and W. F. O. Marasas.
1987. Pathogenecity of fungi isolates from Opuntia
species in Arizona to Opuntia aurantiaca. Phytophylactica 19: 485–489.
Ochoa de Cornelli, M. J., G. Ayrault, C. Degano, and
M. S. Lopez Alsogaray. 1992. El cultivo de la tuna
(Opuntia ficus-indica) en la Provincia de Santiago del
Estero, Argentina. Actas de II Congreso Internacional de
Tuna y Cochinilla. Santiago, Chile. Pp. 97–102.
Mitchell, J. K. 1985. Disorders of natural and commercially grown cacti: A literature review. Cactus and Succulent
Journal (U.S.) 57: 226–231.
Olckers, T., and M. P. Hill (eds.). 1999. Biological control
of weeds in South Africa (1990–1998). African Entomology Memoir 1: 1–182.
Mizrahi, Y., and A. Nerd. 1999. Usage of various cactus
species as fruit and vegetable crops in Israel. Proceedings
of the VII National and VI International Congress on the
Understanding and Utilization of Cactus Pear. San Luis
Potosí, Mexico. Pp. 240–254.
Ortega, J. A. 1991. Alejandro de Humboldt: Ensayo Politico
sobre el Reino de la Nueva España. Editorial Porrua,
Mexico City.
Moran, V. C. 1980. Interactions between phytophagous insects and their Opuntia hosts. Ecological Entomology 5:
153–164.
Moran, V. C. 1983. The phytophagous insects and mites of
cultivated plants in South Africa: Patterns and pest status. Journal of Applied Ecology 20: 239–250.
Moran, V. C., and D. P. Annecke. 1979. Critical reviews of
biological pest control in South Africa. 3. The jointed
cactus, Opuntia aurantiaca Lindley. Journal of the
Entomological Society of Southern Africa 42: 299–329.
Moran, V. C., and B. S. Cobby. 1979. On the life history
and fecundity of the cochineal insect, Dactylopius austrinus De Lotto (Homoptera: Dactylopiidae), a biological control agent for the cactus Opuntia aurantiaca. Bulletin of Entomological Research 69: 629–636.
Moran, V. C., and J. H. Hoffmann. 1987. The effects of
simulated and natural rainfall on cochineal insects
(Homoptera: Dactylopiidae): Colony distribution and
survival on cactus cladodes. Ecological Entomology 12:
61–68.
Moran, V. C., B. H. Gunn, and G. H. Walter. 1982. Wind
dispersal and settling of first-instar crawlers of the
Pemberton, R. W. 1995. Cactoblastis cactorum (Lepidoptera:
Pyralidae) in the United States. An immigrant biological control agent or an introduction of the nursery industry? American Entomologist 41: 230–323.
Perez Guerra, G., and M. Kosztarab. 1992. Biosystematics
of the family Dactylopiidae (Homoptera: Coccinea)
with emphasis on the life cycle of Dactylopius coccus
Costa. Virginia Agricultural Experimental Station,
Bulletin 92–1. Virginia Polytechnic Institute and State
University, Blacksburg. Pp. 1–87.
Pettey, F. W. 1948. The biological control of prickly-pear in
South Africa. Science Bulletin, Department of Agriculture of the Union of South Africa 271: 1–163.
Phaff, H. L., W. T. Starmer, M. Miranda, and M. W.
Millen. 1978. Pichia heedii, a new species of yeasts indigenous to necrotic cacti in the North American
Sonoran Desert. International Journal of Systematic
Bacteriology 28: 326–331.
Phaff, H. L., W. T. Starmer, J. Tredick, and M. Miranda.
1985. Pichia deserticola and Candida deserticola, two
new species of yeasts associated with necrotic stems of
cacti. International Journal of Systematic Bacteriology 35:
211–216.
Pimienta-Barrios, E. 1974. Estudio de las causas que pro-
Insect Pests and Diseases
253
ducen el engrosamiento de cladodios en nopal
(Opuntia spp.) en la zona de Chapingo. Master’s
Thesis, Colegio de Postgraduados, Chapingo, Mexico.
Raabe, R. D. 1979. Some previously unreported hosts of
Armillaria mellea in California III. Plant Disease
Reporter 63: 494–495.
Redfern, D. B. 1968. The growth rate of Armillaria mellea.
Transactions of the British Mycological Society 51: 575–
586.
Redfern, D. B. 1973. Growth and behaviour of Armillaria
mellea rhizomorphs in soil. Transactions of the British
Mycological Society 61: 568–581.
Robertson, H. G. 1987. Oviposition site selection in
Cactoblastis cactorum (Lepidoptera): Constraints and
compromises. Oecologia 73: 601–608.
Robertson, H. G., and J. H. Hoffmann. 1989. Mortality
and life-tables on Cactoblastis cactorum (Berg) (Lepidoptera: Pyralidae) compared on two host-plant
species. Bulletin of Entomological Research 79: 7–17.
Robles, M. A. J. 1999. Respuesta de la grana de cochinilla
en diferentes cultivares de nopal. Proceedings of the VIII
Congreso Nacional y VI Internacional Sobre el Conocimiento y Aprovechamiento del Nopal. San Luis Potosí,
Mexico. Pp. 113–114.
Scheinvar, L. 1999. Biosistematica de los xoconostles mexicanos y su potencial económico. Proceedings of the VIII
National Congress and IV International Congress on the
Understanding and Utilization of Cactus Pear. San Luis
Potosí, Mexico. Pp. 255–274.
Skerman, V. B. D., V. Mc Gowan, and P. H. A. Sneath.
1980. Approved lists of bacterial names. International
Journal of Systematic Biology 30: 225–420.
Stange, G., J. Monro, S. Stowe, and C. B. Osmond. 1995.
The CO2 sense of the moth Cactoblastis cactorum and
its probable role in the biological control of the CAM
plant Opuntia stricta. Oecologia 102: 341–352.
Starmer, W. T., H. S. Phaff, M. Miranda, and M. W.
Miller. 1978. Pichia captophila, a new species of yeast
found in decaying tissue of cacti. International Journal
of Systematic Bacteriology 28: 318–325.
Strong, D. R. 1979. Biogeographic dynamics of insect-host
plant communities. Annual Review of Entomology 24:
89–119.
Strong, D. R., and D. A. Levin. 1979. Species richness of
plant parasites and growth form of their hosts. American Naturalist 114: 1–22.
Tito, S. M. 1998. Plagas y enfermedades del nopal que
254
Zimmermann and Granata
afectan la producción de grana-cochinilla Dactylopius
coccus Costa. Memorias del Primer Congreso Internacional de Grana Cochinilla y Colorantes Naturales.
Oaxaca, Mexico. Pp. 39–40.
Varvaro, L., G. Granata, and G. M. Balestra. 1993. Severe
Erwinia caused damage on Opuntia ficus-indica in
Italy. Journal of Phytopathology 138: 325–330.
Volchansky, C. R., J. H. Hoffmann, and H. G. Zimmermann. 1999. Host-plant affinities of two biotypes of
Dactylopius opuntiae (Homoptera: Dactylopiidae):
Enhanced prospects for biological control of Opuntia
stricta (Cactaceae) in South Africa. Journal of Applied
Ecology 36: 85–91.
Von Arx, J. A. 1984. Notes on Monographella and Microdochium. Transactions of the British Mycological Society
82: 373–374.
Went, F. W. 1982. The physiology of Cacti. In The Cacti of
the United States and Canada (L. Benson, ed.). Stanford
University Press, Stanford, California. Pp. 56–62.
Witbooi, W. R. 1991. Evaluation of Opuntia pathogens obtained from Arizona cacti to Opuntia aurantiaca.
Master’s Thesis, University of Fort Hare, Alica, South
Africa.
Wollenweber, H. W., and O. A. Reinking. 1935. Die
Fusarien. Ihre Beschreibung, Schadwirkung und Bekämpfung. Verlag Paul Parey, Berlin.
Wright, E. R. 1997. Identificación del patógeno productor
de una mancha en cladodios de Opuntia ficus-indica.
Bachelor’s Thesis, Universidad de Buenos Aires, Buenos
Aires, Argentina.
Zimmermann, H. G., and V. C. Moran. 1982. The ecology and management of cactus weeds in South Africa.
South African Journal of Science 78: 314–320.
Zimmermann, H. G., R. E. McFadyen, and H. E. Erb.
1979. Annotated list of some cactus-feeding insects of
South America. Acta Zoologica Lilloana 32: 101–112.
Zimmermann, H. G., and M. Perez-Sandi y Cuen. 1999.
A new insect pest on Opuntiae in wait for Mexico.
Proceedings of the VIII National Congress and the VI
International Congress on the Understanding and
Utilization of Cactus Pear. San Luis Potosí, Mexico. Pp.
333–341.
Zimmermann, H. G., V. C. Moran, and J. H. Hoffmann.
2000. The renowned cactus moth, Cactoblastis cactorium: Its natural history and threat to native Opuntia
floras in Mexico and the United States of America.
Diversity and Distributions 6: 259–269.
CHAPTER
› 15 ‹
B R E E D I N G A N D B I OT E C H N O LO G Y
Brad Chapman, Candelario Mondragon Jacobo,
Ronald A. Bunch, and Andrew H. Paterson
Introduction
Breeding and Biotechnology Research Objectives
Expanding Markets
Breeding
Breeding programs
Germplasm Pools
The Cultivated Gene Pool
The Semidomesticated and Wild Gene Pool
Germplasm Collections
Breeding Systems and Techniques
Controlled Pollination
Clonal Propagation/Apomixis
Future Directions
Biotechnology
Overview of Plant Molecular Tools
DNA and Protein Analysis
Tissue Culture and Transformation
Evolutionary Relationships
Molecular Tools for Cacti
Future Directions
Conclusions
Literature Cited
Introduction
This chapter focuses on the highly important fruit-bearing
group of the genus Opuntia, although the techniques,
methods, and concepts presented are transferable to most
of the Cactaceae. Because cactus germplasm can be improved via plant breeding and molecular techniques, genetic resources and current objectives for breeding and
biotechnology are discussed. A summary of molecular genetic research involving cactus is followed by a plan for utilizing the work from other plant species to advance the
biotechnology of cactus.
The previous chapters leave little doubt about the
uniqueness and usefulness of the Cactaceae; unfortunately, the family has been underutilized as a target for improvement using plant breeding and biotechnological
255
techniques. The multiple uses and the ability of cacti to
thrive in arid and semiarid environments that many other
species are unable to tolerate make it of immediate interest to breeders and molecular biologists seeking to develop crops for areas typically unsuitable for conventional
agriculture. The most obvious trait of interest is the ability of cacti to be productive under water-limited conditions
(Nobel 1988). Coupled to the drought-tolerant phenotype
is the ability of cacti to tolerate high growth temperatures.
Third, another well-studied feature of cacti is their ability
to thrive under elevated atmospheric carbon dioxide levels
(Nobel 1991a; Cui et al. 1993; Nobel and Israel 1994). In addition, cacti have an extremely high productivity under optimal conditions (Nobel 1991b). Cacti are economically
important and physiologically interesting, making them a
worthy and intriguing subject for breeding and molecular
work. Because the cacti used today are primarily wild
plants that have merely been domesticated, advances in
plant breeding and biotechnology could extend the growing range into new environments and could generate new
uses for cactus products. Defining objectives for these investigations will be an essential part of developing organized and productive breeding and molecular biological
programs.
Breeding and Biotechnology Research Objectives
The broad goals of both biotechnology and breeding research for cacti are to understand the molecular mechanisms underlying interesting and useful traits and to use
this understanding to develop crops that are better suited
to human needs. Improvement must be intimately tied to
the needs of growers, processors, and consumers. Because
cacti lack the extensive breeding history of other crops,
many avenues of research on the improvement of cactus
germplasm are possible. The priority of research goals will
vary depending on the production region and the local
uses of cacti. Improvements to cactus varieties will fall into
three main categories: (1) expanding the production area
into new environments, (2) improving the quality and
productivity of cacti to expand into new markets, and (3)
adding new traits to allow the development of new uses for
cacti.
Cacti are well adapted to the arid and semiarid regions
where food and fodder crops are limited, although minimum temperatures substantially limit the growing range in
some areas (Wang et al. 1997). Thus, a primary biotechnological goal should be to expand the production area for
cacti by producing cold-tolerant cultivars. Current highyielding fruit, vegetable, and forage varieties are killed by
256
Chapman, Mondragon, Bunch, and Paterson
temperatures of –5 to –8°C (Loik and Nobel 1991; Parish
and Felker 1995; Wang et al. 1997). Nevertheless, wild
opuntias have a broad geographic range, including southern Canada (Loik and Nobel 1993). Vast areas of the southcentral United States and northern Mexico would be
available to grow cacti if cold tolerance could be introduced to highly productive, high-quality varieties. Because
researchers are beginning to elucidate the mechanisms for
cold tolerance in Opuntia germplasm, the immediate objective should be to understand the genetic basis for this
tolerance and begin to integrate this into commercially
useful varieties with a minimal transfer of undesired characteristics. For fruit production, chilling requirements
may be an important aspect for research. For instance, exposure to day/night temperatures of 15/5°C versus 35/25°C
produces significantly more floral than vegetative buds for
Opuntia ficus-indica (Nobel and Castañeda 1998). Genetic
manipulation of the chilling requirements of temperate
fruit crops is common. Employing similar techniques for
Opuntia species will help expand fruit production areas to
the tropics and subtropics. In addition, out-of-season production would be possible in established cactus production
areas.
Increased disease and insect resistance is probably a
prerequisite for introducing cactus varieties into new regions. In general, cacti are relatively tolerant of diseases and
insects (Chapter 14), although several diseases attack them
(Granata and Sidoti 1997; Saad et al. 1998), and more cactus diseases are likely to appear as cactus production
spreads. Cultivars with both broad-based resistance and
disease specific resistance will be essential for maintaining
consistently high yields.
Various biological questions relating specifically to
cacti potentially have long-term applied value. Understanding the molecular basis of adaptations conferring
drought and high-temperature tolerance has implications
for increasing drought and temperature tolerance in other
crop species and is of general interest for understanding
plant physiology. Similarly, cacti may prove to be important organisms to study the molecular genetics and evolutionary conservation of CAM (Cushman and Bohnert
1997). A greater understanding of cactus polyploidy will be
useful for both basic and applied research. The basal number of chromosomes is n = 11 (Pinkava et al. 1985, 1992).
Many wild species are diploids, although polyploids up to
2n = 19x = 209 or higher occur (Mondragon 1999). The
amazing range of chromosome numbers for cacti presents
an opportunity to study the basis of polyploidy that is paralleled among cultivated plants only by sugarcane. In ad-
dition, understanding the mechanisms behind this polyploid nature may help facilitate crosses between cacti of
different ploidy levels, reducing some of the genetic barriers for hybridization. Immense opportunities exist for
breeding and biotechnological studies of cacti. This chapter outlines specific research that can accomplish objectives
and answer questions discussed above. Areas are emphasized where different disciplines can make important
contributions.
Expanding Markets
Although cactus pears are a coveted fruit by many and a
staple seasonal fruit in some areas of the world, their consumption is generally limited to ethnic groups with a historical association with the fruit. Seeds, which are typically swallowed along with the pulp, are the major deterrent
to attracting new consumers to cactus pear. The seed count
ranges from 80 to more than 300 per fruit (Pimienta 1990;
Barbera et al. 1991), which means there are 3 to 8 grams of
seeds per fruit, depending on fruit size and the cultivar
(Mondragon and Perez 1996). Unbalanced gametes as a result of polyploidy can lead to the partial seed set commonly observed for O. ficus-indica (Nerd and Mizrahi
1994). The ratio between aborted and normal seeds is higher in Italian (0.44) than in Mexican cultivars (0.11;
Pimienta and Mauricio 1987; Barbera et al. 1994). Seed
content is positively correlated with fruit size, and large
fruits command a premium price in the market. Thus the
ideal fruit should have a large number of seeds to attain
good size, but a high ratio of aborted to normal seeds.
The environmental and crop management factors
influencing seed content have not yet been separated
from genetic factors. Parthenocarpy (production of fruits
without seeds because of lack of pollination or fertilization,
or fertilization followed by embryo abortion) has also been
mentioned as a solution to the problem of seediness in the
fruit. Natural parthenocarpy was reported in BS1, a yellow
fleshed accession studied in Israel that does not require pollination for fruit set and development (Weiss et al. 1993;
Nerd and Mizrahi 1994). However, the degenerated seeds
still contain hard arils, and the overall fruit quality is low.
Several attempts have been made to reduce the size of the
seeds by means of gibberellin application but with little
success (Gil and Espinosa 1980; Aguilar 1987; Ortiz 1988).
Fruit size and shape as well as pulp color are important
factors to consider when developing new cultivars of cactus pear. Large fruits are preferred. Also, oval or barrelshaped fruits are easier to harvest than elongated fruits and
therefore suffer less harvest damage to the stem end
(Cantwell 1991). In Mexico, green-white pulp is preferred,
in Italy and northern Europe yellow-orange pulp is preferred, and in the United States and Canada red pulp is
often favored. Pulp juiciness and the high content of soluble solids are considered important fruit quality factors
(Wang et al. 1998). For some markets, fruits with higher
acid content are favored (Saenz and Costell 1990). Postharvest handling, packing, and storage need to be considered when developing new cultivars of cactus pear for commercial use (Corrales-Garcia et al. 1997).
Although spine density and size on the cladodes vary
among accessions (collected wild varieties or cultivars),
some spines are present even in so-called spineless cultivars.
Spineless individuals do not occur in the wild, indicating
that this trait was acquired through domestication. In regard to crop management, spines represent an inconvenience because they hamper routine operations, such as
pruning, and represent an important obstacle at harvest
time. Commercial varieties for fruit in Mexico are spiny
(with the exception of ‘Roja Lisa’). Other producing countries rely on spineless cultivars, and efforts to develop new
varieties should be focused on producing spineless types.
The presence of glochids on the fruit peel is also a
major constraint to increased consumption. Selection and
breeding for glochid-free varieties should be a priority
(Barbera 1995). Another solution is to develop cultivars
with easily shed glochids. Genes for low numbers of areoles
and short glochids are present in O. robusta, although the
fruit of this species is not well accepted due to its low sugar
content, bland flavor, and short shelf life.
For cactus used as a vegetable, the presence of spines
and glochids is one of the main constraints for the development of a wider market outside Mexico. A solution to
this problem is the development of spineless varieties.
Another possibility is to implement early screening for low
number of glochids on the cladodes as well as short, soft
glochids. Moreover, cultivars of Nopalea species produce
cladodes that are nearly free of spines and glochids, which
can help introduce cacti as a vegetable into new markets.
The ability to shed the spines and glochids before the
cladodes age could be a selection criteria when breeding for
new vegetable varieties. Another useful selection criteria
could be the shape of the podarius (tubercle); large podaria
can facilitate mechanical peeling. Suitability of new accessions to be consumed as a vegetable can be evaluated in the
second year, because there is no need to wait until the reproductive stage is reached. Spines, if not properly removed, can also be a problem for feeding livestock.
Breeding for increased nutritional content would be a wor-
Breeding and Biotechnology
257
thy goal to increase the availability of feed in arid and semiarid regions (Felker 1995a).
Breeding
Cactus pear was domesticated in the highlands of Central
Mexico. Reports of consumption of cacti date back to the
ancient groups that inhabited Mesoamerica, and evidence
exists that agaves and cacti have been part of the human
diet for over 9,000 years (Nobel 1994). The transition to
planned cultivation of Opuntia, a crucial step in the early
domestication of wild plants, may have taken place before
8,000 years B.P. (Hoffman 1995). While some traditionally used species (e.g., Steneocereus spp., Hylocereus spp., and
Selenicereus megalanthus) are only now being cultivated,
cactus pear has been farmed for thousands of years. It is,
together with corn, beans, and agave, among the oldest
cultivated plants in Mexico.
Cactus pear developed into a formal crop in the 20th
century as it evolved from collections of wild plants to exceptional hand-selected individuals grown in backyards.
The transition of the rural population toward an urban
semi-industrial society created a consumer base for the initial market of cactus pear in the 1960s. During the 1970s
through the 1980s, programs initiated by the Mexican government provided an important boost to the cactus pear
industry—increasing the area of commercial orchards and
establishing research programs. Selection in the last few
centuries has led to a number of outstanding cultivars with
exceptional fruit quality and productivity, as well as
drought and frost tolerance. The outcome of this longterm activity has resulted in six to eight commercial cultivars as the basic stock for the Mexican and Italian markets,
currently the most important producers and consumers
worldwide.
Breeding Programs
Hybridization of cactus pears was claimed by Luther
Burbank in the early 1900s, leading to the development of
the so-called Burbank spineless cactus with an immense
potential as cattle forage in desert areas. Several varieties
produced from extensive crossing and selection among accessions from Mexico, Africa, Australia, and other countries were developed and aggressively marketed (Dreyer
1985). The lack of records and formal publications gave rise
to questions about the sources of plant material and
methodologies used for the released cultivars. Nevertheless,
Burbank was responsible for the distribution of a tremendous amount of Opuntia germplasm throughout the
world, such as the cactus pear cultivars found today in
South Africa (Wessels 1988).
258
Chapman, Mondragon, Bunch, and Paterson
Modern-day cactus pear breeding in Mexico began in
1964 when the late Facundo Barrientos of the Colegio de
Postgraduados, Chapingo, began his program for its genetic improvement. The ambitious program included
germplasm collection and utilization, breeding of new cultivars, and development of new uses for cactus pear. He
envisioned that the ideal cactus pear cultivar should be
multipurpose and widely adapted, and he worked toward
high fruit quality, high forage quality, drought and heat resistance, and early maturity. In the early years he introduced selections ‘COPENA V1’ and ‘F1’ for vegetable and
forage, respectively. He developed a series of high-quality
white (or green) pulped selections (the ‘COPENA T’ series) that had only modest commercial success as they did
not present a clear advantage over the native white cultivars. Later, he broadened his efforts to include other colored cactus pears and other species of fruiting cacti. The
red pulp cultivar ‘Frieda’ or ‘Torreoja’ was his last released
cultivar. Prior to his untimely death in 1993, Dr. Barrientos
interacted with cactus growers and researchers throughout
the world and shared with them germplasm and breeding
methodologies. He created a spark that ignited the current
interest in new cactus varieties and cactus breeding.
A fresh start at breeding is now underway in the
United States, South Africa, Israel, and Mexico. Encouraged by the Food and Agriculture Organization of the
United Nations, a renewed interest in the collection of wild
and semi-domesticated accessions has developed. In addition, the number of publications with information on crop
management practices and new uses of cactus pear are
increasing.
Germplasm (gene pool) collection and characterization
is the major field of interest in most of the Mexican institutions engaged in cactus pear research, reflecting the fact
that Mexico is the main reservoir of variability of edible
opuntias. A special concern is the rapid disappearance of
valuable plant material from wild stocks in northcentral
Mexico resulting from the intensive utilization by the cattle and dairy industry. Backyard orchards have been
searched for outstanding individuals to widen the limited
array of commercial cultivars for fruit and vegetable production. Three state universities and one technical school
support these germplasm collection programs. Formal
breeding programs, however, are conducted only by the
Instituto Nacional de Investigaciones Forestales y Agropecuarias (INIFAP) and the Colegio de Postgraduados.
Their breeding efforts are mostly focused on the development of spineless cultivars for fruit production. Potential
expansion into new national and foreign markets emphasizes the development of colored, juicy fruits with low seed
content, early fruiting habit, high soluble solids, and tolerance to indigenous pests and diseases. Vegetable and fodder cultivars are also a priority. The recurrent frosts and
droughts observed during the last decade are prompting
the search for new cultivars with sustained productivity
under such conditions. Programs rely on hybridization and
selection as breeding tools. The germplasm base for these
projects is totally indigenous, and large populations of hybrids and segregants have been in field trials since 1994.
Two institutions in Mexico are pioneering the application of molecular techniques to cactus pear breeding, the
Centro de Investigación y Estudios Avanzados (CINVESTAV) in Guanajuato and the Instituto Tecnológico
Agropecuario (ITA 20) in Aguascalientes. Their investigations are directed toward the study of enzymes involved in
cell wall softening, with the goal of controlling ripening
and thus avoiding temporal saturation of the national market. Other areas of research interest include seed storage
proteins (Silos-Espino et al. 1999), cactus transformation,
and gene transfer to increase protein content. Beginning in
the 1980s, Texas A&M University-Kingsville became involved in agronomic research, extension work, and collection and introduction of cactus pear to the United States.
The program aims to develop frost-tolerant cultivars to
overcome the common limitation to growing cactus in this
region. In 1996 the first round of crosses was initiated,
marking the beginning of a long-term breeding program.
This institution is also responsible for the popularization
of the vegetable cultivar ‘Spineless 1308’ and various cactus
products in Texas.
D’Arrigo Brothers, a produce company based in
California, started a private breeding program in 1994 to
improve their spineless commercial cultivar ‘Andy Boy,’
which is similar to the ‘Rossa’ cultivar grown and marketed in Italy and currently dominates the American out-ofseason market. The main objective of this program is the
improvement of fruit quality. The program utilizes
germplasm obtained from Dr. Barrientos and has recently
added a portion of the Texas A&M University-Kingsville
collection as breeding material. Seedlings from the first
crosses were planted into the field in 1997. Other active
programs include one located in Sassari, Italy, that is involved in the improvement of fruit quality using naturalized accessions collected in the semiarid Mediterranean region of Italy. Field trials of crossed and open pollinated
seedlings were initiated in 1994. In Argentina, evaluation
trials of local and introduced accessions are being conducted. In addition, an ongoing collaborative project with
the University of Georgia is developing a molecular marker map from progeny of a cross between two Texas A&M
accessions. In South Africa, extensive trials are being conducted to evaluate the best local cultivars. In addition, a
study has begun utilizing random amplified polymorphic
DNA (RAPD) markers to identify cultivars of plant material being sold to growers in that country. In Israel, evaluation of crosses and selections of fruit-producing Cereus,
Hylocereus, and Selenicereus species is underway as part of
a large project to develop new fruit crops for that country.
Germplasm Pools
Each of the major germplasm pools (cultivated, semidomesticated, and wild) exhibits traits of interest for the genetic improvement of cactus pear. A wide range of ploidy
levels exists among and within the Opuntia species.
Pimienta and Muñoz (1995) compiled data from several authors and list reported ploidy levels of 2x, 3x, 4x, 5x, 6x, 8x,
10x, 11x, 12x, 13x, 19x, and 20x. The cultivated types of
cactus pear generally have higher chromosome numbers
(2n = 6x = 66 and 2n = 8x = 88) than the wild populations
(usually 2n = 2x = 22 and 2n = 4x = 44). Although large
crossing studies have yet to be conducted, barriers to cross
compatibility are minimal among the cultivated species.
Natural hybrids are common in both cultivated and wild
populations of cactus pear (Pimienta and Muñoz 1995).
Trujillo (1986) obtained viable seeds from crosses of wild
O. streptacantha with O. robusta and O. leucotricha with O.
cochinera. Wang et al. (1996) successfully crossed O. lindheimeri with several cultivated cactus pears. In addition, O.
robusta and cultivated cactus pear have been crossed.
The Cultivated Gene Pool
Cultivated species of cactus pear include Opuntia ficusindica, O. albicarpa, O. streptacantha, and O. robusta plus
hybrids between O. ficus-indica and the others (Pimienta
and Muñoz 1995). For breeding purposes, this germplasm
pool is best sorted out by cultivar or specific traits of interest rather than by taxonomic species. Modern cultivars
of cactus pear are the products of long-term informal, but
effective, selection by growers. The most important selection criteria have been fruit size and quality, plant productivity, and tolerance to drought and frosts. The number of cultivars in each of the countries that grow cactus
pear varies according to the intensity of usage and the size
of the initial germplasm base, with Mexico accounting for
the largest diversity.
Three main groups of cactus pear can be recognized according to the color of the peel and pulp: white (or light
green), yellow (including deep orange), and red (from light
red to deep purple). The most popular white cultivars in
Mexico are ‘Reyna,’ ‘Cristalina,’ ‘Esmeralda,’ and ‘Bur-
Breeding and Biotechnology
259
rona.’ ‘Reyna’ sets the standard of quality and dominates
the national market (Mondragon and Perez 1993). The national demand in Mexico is supplemented by the orangepulped ‘Naranjona’ and ‘Amarilla Montesa’ and the redpurple ‘Roja Lisa’ (Mondragon and Perez 1993, 1996;
Pimienta and Muñoz 1995). In the United States, the redfruited cultivar ‘Andy Boy’ is available from September
until April; it is produced only in California where irrigation and mild winters facilitate out-of-season production.
In Chile, a cultivar of O. ficus-indica known as ‘Verde’
(Green; Sudzuki 1995) or ‘Blanca’ (White; Pimienta and
Muñoz 1995) is the most common; since 1982, exports to
the U.S. market take advantage of the summer harvest season in the Southern Hemisphere, which coincides with
winter in the Northern Hemisphere. The cultivars available in the Sicilian area of Italy are ‘Gialla’ (Yellow),
‘Bianca’ (White), and ‘Rossa’ (Red), with ‘Gialla’ being the
most common (Barbera et al. 1992). A ‘seedless’ cultivar is
also known but its commercial cultivation has never been
attempted because of the poor quality of the fruits.
Production in Israel is based mostly on the cultivar ‘Ofer,’
which has yellow pulp. In South Africa the varieties available, 40% of which have light-green fruit, originated
from 21 spineless types imported from the Burbank
Nursery of California in 1914. Besides color, varieties also
differ in other pulp characteristics, peel features, postharvest physiology, and response to environmental factors.
Because the vegetable nopalitos are most widely consumed in Mexico, Mexico is the main source of germplasm. The Universidad Autónoma de Chapingo near
Mexico City has assembled an extensive collection; ‘Milpa
Alta’ (O. ficus-indica) is the most important cultivar and is
cultivated mostly in the region of the same name near
Mexico City. ‘COPENA V1’ is perhaps the second most
important vegetable cultivar. Another variety, ‘COPENA
F1,’ was selected for fodder production by Dr. Barrientos
in the 1960s, but its tender pads are also suited to consumption as a vegetable. Both have intense green color, a
thin epidermis, good flavor, and low acidity (Flores 1995).
‘Moradilla,’ ‘Atlixco,’ ‘Polotitlan,’ and ‘Redonda’ are further examples of locally selected varieties of O. ficus-indica
used for vegetable production. In the southwestern states
of Michoacan and Jalisco, the ‘Nopal blanco’ or white cactus pear is the cultivar of choice for nopalitos and tolerates
humid conditions (up to 1,600 mm of rainfall annually).
The cultivar ‘Valtierrilla’ is used as a vegetable in central
Guanajuato if picked very young. The cultivar ‘Spineless
1308’ (O. cochellinifera or Nopalea cochellinifera), selected
by Peter Felker from accessions collected in a humid tropical region of Tamaulipas, Mexico, is suitable for field cul-
260
Chapman, Mondragon, Bunch, and Paterson
tivation in the coastal rain-fed areas of Texas and for greenhouse cultivation in frost-prone areas near San Antonio.
The Semi-Domesticated and Wild Gene Pool
A remarkable diversity of locally known semi-domesticated types exist in the native areas of cactus pear, some serving two or even three purposes: fruits, tender pads, and
fodder. Traditionally cactus pear plantings are found on
small family properties in dry regions. Opuntia hedges are
concentrated near farmsteads, where they also protect fruit
and vegetable gardens. Taxonomically these cactus pear
plants are similar to various hybrids in the cultivated
germplasm and represent the transition from wild plants to
modern commercial cultivars. Their genetic variability is
important as a source for new and valuable traits that may
be necessary as breeding programs proceed (Hoffman
1995). Outstanding accessions in family orchards provide
a source of individuals derived by chance from free natural outcrossings, whose germplasm has only been minimally collected and characterized.
The last reservoir of interesting individuals is wild populations. Several efforts have been made to collect representatives from the wild populations of fruit-producing
Opuntia species in Mexico. In 1993, Mexico, Israel, and the
United States joined efforts to collect in the highlands of
northern Mexico and assembled 130 accessions of fruit-producing Opuntia species, selected primarily for cold hardiness (Felker 1995b). Similar endeavors have collected several accessions of the hardy Texas native Opuntia lindheimeri.
Crosses among many of these species have been successful
(Wang et al. 1996), although little information is available
for crosses outside of this limited germplasm.
Germplasm Collections
Germplasm collections of cactus pear are maintained at
several locations around the world (Table 15.1). The largest
number of collections and of entries are located in Mexico,
where the greatest diversity occurs for native cactus pear. In
addition, each breeding project and cactus pear research
program has a collection of at least some of this material
(often combined with local accessions).Taben15l.eahrere:
Due to the genetic makeup of cactus pear, its longlived perennial habit, and large plant size, the maintenance
of germplasm banks is difficult and costly. Collecting plant
material based on morphological traits and common local
names often leads to duplicated accessions within a collection. The morphology of Opuntia species is greatly
influenced by the environment; their rapid growth means
their reactions to environmental changes are also fairly
rapid and more drastic than other cacti. For instance, traits
TA B L E 1 5 . 1
Major cactus pear germplasm collections
Institution
Collection locations
Approximate number of
entries and description
Contacts
Mexico
Instituto Nacíonal de
Investígaciones Forestales,
Agrícolas y Pecuarías
(INIFAP)
Palma de la Cruz, San Luis Potosi;
San Luis de la Paz, Guanajuato;
Sandovales, Aguascalientes;
Tecamachalco, Puebla; Ensenada,
Baja California Norte
800 entries, mostly from central Mexico,
commercial cultivars, and backyards;
95% fruit types, 5% vegetable
Dr. Candelario Mondragon
J. or M. C. Rafael
Fernández M.
Universidad Autonoma
de Chapingo
Chapingo, México;
El Orito, Zacatecas
160 entries with a wide variety of cultivars
from all over Mexico; mostly for fruit,
~24 for vegetable, and some for fodder
Ing. Claudio Flores Valdez and
Dr. Clemente Gallegos F.
Secretaria de Educacion Publica (SEP)
Universidad Autonoma
de Nuevo Leon
Villa Hidalgo, Zacatecas
Marin, Nuevo León
90 entries of various Mexican cultivars
110 entries; many for vegetable
Ing. Eloy Rodríguez
Dr. Rigoberto Vázquez A.
United States
Texas A&M University,
Kingsville
Kingsville, Texas
180 entries of cultivated and wild plants
from northern Mexico, southwestern
United States, and a few other countries;
mostly fruit, some for vegetable and forage,
including cold hardy types
Dr. Robert Morgan
South Africa
Department of Agriculture,
Land and Environment
Pietersburg, Northern Province
80 entries of South African cultivars, selections,
and chance hybrids from Burbank material
Mr. Johan Potgieter
Italy
University of Palermo
Palermo
45 entries from Italy, South Africa,
Chile, and Argentina; fruit
Dr. Paolo Inglese
Dipartimento di Colture
Arboree di Università di Sassari
Sassari
Fruit
Dr. Innocenza Chessa
such as spininess, cladode shape and size, fruit characteristics, and plant productivity are affected by the environment (Weniger 1984). Duplication within collections is
very common and complicates the evaluation and utilization of the genetic resources. A major cause of the duplication among collections is that each research group may
maintain separate stocks to have access to as much genetic variability as possible. Increased characterization of
these collections is vitally important to the success of cultivar improvement in cactus pear; however, data collection
and analysis for these collections will be quite expensive.
Only limited exchange of cactus germplasm has taken
place since 1992, when the international cooperation regarding cactus pear formally began. More flexible exchange
of germplasm among countries is restricted due partly to
the lack of accessible data from the collections and partly
to regulations. Despite these limitations, basic descriptions
are available for the most important cultivars and outstanding accessions from Mexico (Pimienta and Muñoz
1995; Mondragon and Bordelon 1996), Italy, South Africa,
Argentina, and Chile (Pimienta and Muñoz 1995).
An important component of developing successful
cactus breeding programs will be utilizing the diversity
available. Thus methods for classification of this diversity
will need to be developed concurrently with cactus breeding programs (Mondragon 1999). Principal Component
Analysis is a powerful multivariate statistical technique to
group and visualize the relationship among cultivars
based on a number of morphological measurements
(Hillig and Iezzoni 1988). Such analysis of 32 cactus pear
accessions from central Mexico based on 17 measured
traits (Mondragon 1999) indicates a positive relationship
between cladode width and fruit weight. This can be used
in breeding programs to select for fruit size in juvenile
plants, instead of waiting until a plant reaches maturity. A
positive correlation also exists between the number of areoles on the cladodes and the number of areoles on the
fruit (Mondragon 1999). Three groups incorporating
eight of the measured traits are the most important in describing the cultivars: plant vigor, frequency of areoles,
and spininess, which together encompass 71% of the variability among the plants. The 32 accessions fall into
seven categories: wild accessions, small-fruited plants,
“spineless,” improved varieties, varieties cultivated for fruit
production, very spiny accessions, and a single cultivar
‘Cristalina,’ that showed extremely vigorous growth. This
approach for characterizing accessions will facilitate the
incorporation of available germplasm into breeding programs by allowing researchers to narrow their focus to single accessions within each major category.
262
Chapman, Mondragon, Bunch, and Paterson
Breeding Systems and Techniques
Cactus flowers are typically relatively large and hermaphroditic with abundant stamens, a single style, and showy perianth parts (Nerd and Mizrahi 1997). The structure of
these flowers suggests that they are cross pollinating and, in
fact, insect pollination is quite common. For cactus pear
under natural conditions, pollination by insects (mainly
bees) ensures a high number of seeds once the flower opens.
In the fruit-producing Opuntia species, self-compatibility
and self-pollination occur, and bagged flowers can set fruit
(Nerd and Mizrahi 1997). Protandry can explain selfpollination prior to flower opening. Little information is
available regarding the ability of wild cactus species to selfpollinate. Bagged flowers of O. lindheimeri did not produce fruits in one study (Grant et al. 1979). However, five
out of ten bagged flowers of O. lindheimeri set fruit and
produced seed in another study (Wang et al. 1996). With
regard to other species of fruiting cacti, clones of Cereus peruvianus, Hylocereus costaricensis , and H. polyrhizus are selfincompatible and those of H. undatus and Selenicereus
megalanthus are self-compatible. Sicilian cultivars of cactus
pear are self-compatible, as problems of fruit set are seldom
encountered in vegetatively propagated plantations composed of a single cultivar or in single plants grown in backyards (Nerd and Mizrahi 1997). Similarly in the Pyramids
region of central Mexico, about 7,000 ha of the cultivar
‘Reyna’ are cultivated without apparent pollination
problems.
Controlled Pollination
Short-term pollen storage for less than a week can be accomplished by collecting buds close to flowering and placing them in a cool and shaded location. Before use, the
buds are exposed to full sun for a few hours to promote
flowering. Unopened buds can also be used, but their
pollen yield is lower. Pollen collected fresh or stored at
room temperature for up to 6 days will remain viable and
effect successful pollination (Bunch 1997). To facilitate germination, five milligrams of cactus pollen are suspended in
5 ml of germination medium prepared with 100 ppm
H3BO4, 300 ppm Ca(NO3)2 . 4H2O, 200 ppm MgSO4,
and 100 ppm KNO3 in a 40% sucrose solution.
The emasculation (removal of the stamens) of a cactus
pear flower resembles a surgical operation and as such
should be performed carefully (Fig. 15.1). The material
needed includes rubber gloves, a brush, a sharp knife or
razor blade, small scissors with a bent tip, a rinsing bottle,
paper towels, glassine or paper bags, and rubber bands.
When emasculating flowers of cactus pear, take the fol-
A
C
B
D
Figure 15.1. Methodology for emasculating flowers of cactus pear: (A) emasculating an Opuntia ficus-indica flower; (B) washing the emasculated
flower; (C) covering the emasculated flower with a bag; and (D) pollinating the emasculated flower with an open flower.
lowing steps: (1) clean the exterior of the buds with the
brush to allow easy handling; (2) excise the corolla, using
as few strokes as possible, avoiding wounds and mechanical damage to the style; (3) carefully remove the stamens
and anthers, cutting close to the base; (4) rinse thoroughly with clean water to get rid of pollen residues and anthers; and (5) cover the emasculated flower bud with a bag
to prevent unwanted pollination.Fgiuren15.eahrere:
Although it is important to minimize damage to the
flower during emasculation, the flowers are able to recover from some damage to the receptacle without significantly harming reproductive potential. The wound response of cactus involves the abundant flow of mucilage
from the wounds. This mucilage covers the wound and
dries, thereby reducing water loss and preventing infections. It is important that the stigma and style remain intact and undamaged. Even young flowers can be emasculated without appreciable loss of fertility. However,
handling very young buds is difficult, because the stamens
tend to be less exposed and the risk of mechanical damage
to the stigma and style is greater. The application of
Dithyocarbamate (Sevin, 2%) powder in the receptacular
cavity after partial emasculation can prevent insect visitation and undesired pollination (Wang et al. 1996).
After emasculation, the stigma becomes receptive in 3
to 4 days; however, under warm (> 35oC) conditions, it is
receptive earlier. When the stigma is receptive, it is shiny
and sticky and the lobes are wide open. The most efficient
way to pollinate is using a detached, fresh, fully open
flower devoid of its style and corolla to allow close rubbing
of the stamens with the stigma of the female flower. Stored
pollen can be applied onto the stigma of an emasculated
flower with a #3 camelhair paintbrush (Bunch 1997). Partially opened buds can also be used, taking advantage of
the protandric nature of cactus pear pollination. Longer
availability of flowers for crossings can be accomplished by
eliminating the first flowering flush, which ensures that
after 50 to 70 days a new round of crosses can be per-
Breeding and Biotechnology
263
formed. In general, crosses performed later in the flowering
season reduce the number of normal seeds. Typically 100
to 250 normal seeds can be obtained from a single fruit, depending upon the crossing conditions and the cultivars involved in the cross. The difficulty involved in emasculation
and the high number of seeds that can be expected from a
single fruit underscores the importance of having a few
carefully performed crosses rather than numerous potentially unsuccessful ones.
Self-pollination can easily be performed by bagging
unopened cactus flowers to avoid cross pollination.
Moving pollen from the anthers to the stigma of the same
flower often results in increased seed set. Typically, inbreeding of a cross-pollinated and vegetatively propagated
crop does not result in superior progeny, although this has
not been confirmed for cacti. In addition, self-pollinated
progeny will help elucidate genetic factors controlling important traits by unlocking genes hidden behind heterozygosity in these out-crossing species. Trials are currently
underway to document differences among various breeding methods.
Seeds should be extracted from ripe and healthy fruits.
Fruits are peeled and processed in a blender at low speed.
Seed disinfection is accomplished by soaking the seeds in
commercial bleach (5–6% sodium hypochlorite) for 10
minutes. Seeds can be dried in an oven for 2 to 3 hours at
55 to 60oC. Seeds obtained in the same season can germinate after slight scarification. Dry storage (6–14 months)
increases the rates of seed germination of several types of
cactus pear (Mondragon and Pimienta 1995). Seed viability is reduced after long-term storage. For instance, storage
of seed lots for 9 years reduces the seed germinability by
50% (Muratalla et al. 1990). Temperature is the most important variable for cactus seed germination. For nineteen
species of cacti the optimal temperature for seed germination ranges from 17 to 34oC with a mean of 25oC (Nobel
1988). Differences in germination have also been attributed
to cultivar and seed condition. Seeds may have a physical
dormancy due to the hard seed coat, which hinders germination. Several treatments have been reported to overcome this barrier. Scarifying seeds in hot water (80–90oC)
twice and allowing them to cool to room temperature and
then soaking them in distilled water for 24 hours promotes
germination under greenhouse conditions in 10 to 17 days
(Mondragon 1999). Seeds soaked in gibberellic acid (35
mg/liter) can germinate faster (Wang et al. 1996). Planting
media should be kept moist for optimal germination of
cactus pear seeds under greenhouse conditions.
Seedlings can be kept in germination trays until the
first cladode grows to 10 to 15 cm. At this size, they can be
264
Chapman, Mondragon, Bunch, and Paterson
transplanted to small pots (10 cm in diameter) or black
plastic bags. Prior to transplanting, the entire tap root can
be excised and the seedling allowed to dry for 5 to 7 days
to promote healing and drying of the wounded tissue.
Elimination of the tap root eases the task of transplanting
and promotes formation of lateral roots, increasing the
root volume and improving anchorage. Minimizing the
time from crossed seed to a mature plant, ready for evaluation, is important for any breeding program. Cacti generally grow slowly, especially the columnar and spherical
forms, under natural conditions. Most cacti respond to optimal conditions of water and nutrients (Nobel 1988).
Other factors influencing this response include photoperiod (Sanderson et al. 1986) and atmospheric CO2 concentration. Six-month-old Opuntia seedlings grown in a
greenhouse can be transplanted to the field. At this age
they may bear two or three slender pads and can be managed as adult plants or used as a source of vegetative material for grafting.
Plants of cactus pear derived from seeds tend to grow
in an upright slender manner, branching only in the upper
cladodes. By comparison, plants grown from cuttings
tend to have thicker and wider cladodes as well as more
pads on the lower part of the plants. Branching can be promoted in seedlings by pinching at the one-cladode stage.
This practice encourages thickening of the basal cladode as
well as branching, thus increasing photosynthetic area and
improving anchorage and vigor. Pinching also allows for an
early expression of adult cladode shape. Grafting is a potential method of reducing juvenility. By grafting immature cladodes onto mature cladodes, the scion (the detached plant part used in a graft) may have at least one
flowering cladode in the next growing season, in comparison with the 4 to 6 years needed for seedlings transplanted to the field directly from the greenhouse.
Grafting is a standard practice to maintain rare forms
of cacti, such as those lacking chlorophyll, cristate forms
(Haage 1963; Pilbeam 1987; Pimienta and Muñoz 1995),
and endangered species. Most information available pertains to spherical forms being used as scions and sharp angled forms of Cereus, Hylocereus spp., Myrtillocactus geometrizans, Pereskia, Rhipsalis, and Trichocereus being used
as stocks (Pizzeti 1985). Grafting platyopuntias is a little
more complicated. The main concern for Opuntia species
is the shape and thickness of the stocks and scions. The
best species to use as rootstocks is O. ficus-indica, because
the plants are fast growing and almost spineless. Cultivars
such as ‘Seleccion Pabellon’ available in Mexico for vegetable and forage production and ‘Gialla’ of Italy are also
well suited for this purpose. Grafting can be performed
with young, full-size cladodes. The scion and stock should
be selected and cut to match for size, shape, and thickness.
To create matching shapes, both the donor cladode and the
rootstock can be cut with the same device (e.g., an aluminum can with one edge sharpened to cut and act as a
template at the same time). The scion should be placed
carefully, trying to match the cambial tissues as closely as
possible and maintained in place using rubber bands.
Clonal Propagation/Apomixis
New and superior cultivars need to be propagated as rapidly as possible for further trials and commercial production. Asexual propagation can be performed for cactus pear
using stems (or portions thereof ) of any age as well as
flowers and unripe fruits. Commercial orchards typically
rely on large pad fractions, whole pads, or short branches.
A few specialized propagation nurseries have been attempted in Mexico with limited success, as cuttings are
most often obtained from productive orchards using pruning residues. Efficient protocols for in vitro propagation are
also available (Escobar et al. 1986; Villalobos 1995), but
they are not used on a commercial scale due to the ease and
lower cost of propagation from cladodes.
Apomixis, the formation of asexually derived embryos, is common in the genus Opuntia (Mondragon and
Pimienta 1995). Polyembryonic seed percentages range
from 0 to 50% for several Opuntia species and from 2 to
16% for several wild and cultivated cactus pear accessions.
From a breeding perspective, polyembryony can be a problem during germination of crosses, because seedlings derived from somatic tissue will be identical to the female
parent as opposed to a combination of the two parents. In
general, identification of apomictic seedlings is relatively
easy in the early stages of germination and seedling growth;
however, some apomictic seedlings are indistinguishable
from the zygotic seedling, and they tend to appear more
similar as they become older. The apomictic seedling usually emerges later, is smaller, and is weaker than the zygotic seedling. Seedlings from polyembryonic seeds must be
separated early in the germination phase of a program and
some somatic seedlings will probably enter the evaluation
phases of a breeding program (Mondragon and Bordelon
1996; Bunch 1997).
Future Directions
Cactus genetic research is in its infancy, and the vast majority of breeding work lies ahead. Several people who were
fortunate enough to have worked with Dr. Barrientos have
retold a story of his: you can cross two cactus pear varieties
and obtain five species in the progeny. This story attests to
the tremendous variability available among cactus pear and
to the amount of work needed to sort out and harness this
variability. With the increasing number of new and welldocumented crosses performed in cactus programs around
the world, the genetics behind many traits should be gradually revealed. Information from these crosses will help sort
out taxonomic relationships and lend insight into the genetic control of some of the special characteristics and
physiological systems that make cacti such remarkable
plants (Nobel 1994).
As breeding efforts are continued, new and exciting research will unfold. Future projects may involve creating
ploidy series of cacti of a specific genetic makeup. Also, in
keeping with the sustainable nature of cacti and their potential to benefit regions of the world with limited resources, nutrient utilization of new cultivars must be investigated. Cultivars should be developed with enhanced
ability to benefit from associations between rhizosphere organisms to increase productivity and reduce dependence
on fertilizers. New biotechnological tools will help breeders meet their objectives as well as allow access to new
sources of genetic variation. The ultimate goal will be to
develop cacti into crops rivaling the best of other fruit, vegetable, and forage crops.
Biotechnology
An important complement to cactus breeding programs is
the development of molecular tools to work with cacti. As
has been demonstrated for numerous crop species, molecular biological tools can speed up the breeding process
(Paterson et al. 1991; Staub et al. 1996), elucidate genetic
mechanisms that cannot be easily dissected through plant
breeding techniques (Paterson 1995), and in many cases can
accomplish goals that are not possible through breeding.
This section reviews the molecular work done on cacti and
proposes a plan for the development of molecular tools for
cacti utilizing the work done on traditional crop species.
Overview of Plant Molecular Tools
Most plant molecular genetics has focused on economically important crop species, such as corn or rice, or on
facile models, such as Arabidopsis. The work done on other
species can serve as a springboard to speed development of
such tools for cacti. In turn, genetic analysis of cacti would
pave the way for developing molecular programs for alternative crop species. A well-developed genetic map is important in this regard (Paterson et al. 1991; Staub et al.
1996). Genetic maps are constructed by utilizing highly
variable, polymorphic molecular markers to assess levels of
recombination in a cross between two variable parents. The
Breeding and Biotechnology
265
molecular markers serve as landmarks along the chromosomes of the organism and reflect underlying differences in
the genomes of the two plants involved in the cross. Both
hybridization-based markers, such as Restriction Fragment
Length Polymorphisms (RFLPs), and PCR-based markers,
such as RAPDs and Microsatellites, are routinely used. The
ability of molecular markers to detect subtle changes in
genome structure make them highly advantageous over
traditional morphological markers for developing genetic
maps of cacti. If a breeder attempts to move a cold-resistant
phenotype from a wild Opuntia accession into a specific
cultivar and lacks a molecular marker associated with cold
resistance, all plants need to be grown to maturity and then
tested for cold resistance. By contrast, if cold resistance is
associated with a molecular marker, this marker can be
screened in young plants, thus helping the breeder to make
selections earlier in development.
Another important tool for molecular work is the ability to transform cactus plants with DNA fragments of interest. Although progress has already been made in this
area (Zárate et al. 1998, 1999a), many hurdles remain. An
important step is to generate stably transformed plants that
can transmit the transgene of interest to subsequent generations through sexual crosses. For instance, if a gene conferring cold tolerance is isolated, it can be integrated into
cultivated plant germplasm by transformation without
having to worry about traditional breeding difficulties,
such as making wide crosses or removing unwanted wild
germplasm introduced into a cultivar when it is crossed
with the wild plant. This is especially important for cacti,
as the time from seedling to mature plant can be long,
making the production of backcross populations time consuming. In addition, transformation allows genes to be
transferred from plants that cannot be crossed with cacti,
or even from other organisms. For instance, if one or a few
genes controlling seediness can be identified, these genes
could be inactivated through transformation of anti-sense
constructs and screening for transformed plants that silence the seediness-related genes.
DNA and Protein Analysis
Molecular work done on cacti reveals a number of intriguing features. The polyploidy level varies, with a basal
chromosome number of 11 (Pinkava et al. 1985, 1992).
Analysis of nuclei from Opuntia TAM 1308 by flow cytometry indicates that they possess a DNA content similar to that of small genome crops such as sorghum and
tomato. Isozyme analysis detecting variability among
plants based on activity of known enzymes indicates a high
level of variability among cacti, especially in pollen (Chessa
266
Chapman, Mondragon, Bunch, and Paterson
et al. 1997). Other isozyme studies show a low variability
among accessions but a curiously high variability among
fruits and cladodes of the same cultivar (Uzun 1997). In
contrast to isozyme studies, RAPD assays the variability of
unknown DNA sequence fragments amplified using the
Polymerase Chain Reaction (PCR). RAPD markers were
used to verify the somatic origin of putative apomictic
seedlings using seeds from two crosses of Mexican accessions (Mondragon 1999). The study indicated that seedling
size and fresh weight are the only morphological features
associated with the apomictic seedlings.
RAPDs have also been employed to compare DNA
variability for relationships among Opuntia species with
traditionally derived morphological classifications (Wang
et al. 1998). Discrepancies occur between molecular and
traditional classifications, although more work needs to be
done to characterize these differences. A DNA extraction
technique for cacti, which helps overcome the difficulties
caused by mucilage, has been used for 32 accessions
(Mondragon 1999).
Tissue Culture and Transformation
An efficient micropropagation protocol for cactus pear has
been developed (Escobar et al. 1986). In particular, in vitro
culture can produce virus-free plants and is a method for
rapid propagation of new cultivars. The technique has not
been used on a commercial scale due to the comparative
ease of propagation through traditional methods. The production of somatic embryos (asexually formed embryos
that arise from sporophytic cells unconnected with maternal tissues) occurs for a number of cactus species in tissue
culture (Torres-Muñoz and Rodríguez-Garay 1996;
Santacruz-Ruvalcaba et al. 1998). Ongoing work at the
Federal University of Ceará in Brazil has led to a number
of advances in both tissue culture and transformation techniques, such as callus and cell suspension cultures of
Opuntia (Zárate et al. 1999c), cultivation of isolated shoot
meristems, and regeneration of whole plants from the apical meristem (Zárate et al. 1999b). These tissue culture
techniques have produced transient transformation of a βglucuronidase reporter gene in callus and cell suspension
cultures (Zárate et al. 1998) and in apical meristems
(Zárate et al. 1999a). Analysis of seed reserve proteins in
Opuntia ficus-indica (Uchoa et al. 1998) will be useful to research on improving cactus cultivars through transformation techniques.
Evolutionary Relationships
Much information from other plant species can be used for
developing molecular tools for cacti. Understanding these
relationships will be key to using comparative mapping
techniques to speed the development of a molecular map
for cacti. Molecular sequence data for the plastid gene encoding the large subunit of Rubisco (rbcL) were obtained
for a number of plant species, including some in the Cactaceae, from Genbank (http://www.ncbi.nlm.nih.gov/).
The sequences were aligned using the Clustal alignment
program (http://www-igbmc.ustrasbg.fr/BioInfo/ClustalX/
Top.html), and phylogenetic trees were calculated via the
parsimony method using DNAPars (available as part of the
Phylip molecular phylogeny package; http://evolution.genetics.washington.edu/phylip.html). A consensus tree created using the majority-rule method (CONSENE, also in
the Phylip package) indicates that the Cactaceae are most
closely associated with the Aizoaceae. A well-characterized
member of this family is Mesembryanthemum crystallinum,
which also utilizes the CAM photosynthetic pathway.
Closely associated to the Cactaceae are the Poaceae (grasses) and the Solanaceae. This tree is biased by the use of a
gene involved in the photosynthetic machinery, which
likely varies between CAM, C3, and C4 species. No phylogeny based on variation in a single gene can characterize
the relationships between such a broad class of plant families. Hopefully, future molecular phylogenetic work will
refine the position of the Cactaceae among other plant
families.
Molecular Tools for Cacti
A first step in building molecular tools is to identify cactus sequences that relate to potentially useful genes in other
taxa. This can be accomplished by hybridizing cactus
clones with probes from other taxa and looking for expressed genes that are highly similar. To achieve this goal,
a cDNA library will be constructed from Opuntia accessions from Texas A&M University-Kingsville. Genes recognized in other organisms involved in critical processes
for cacti will be utilized. For example, cold-temperaturetolerance genes have been identified in Arabidopsis
(Thomashow 1998) that might allow recognition of cactus
genes involved in the same process. Other possibly useful
genes to identify include those involved in drought/salt tolerance (Winicov 1998) and those uniquely involved in
CAM (Cushman and Bohnert 1997).
The second step in developing cactus molecular tools
will be to create a genetic map with the useful sequences
providing the DNA markers. A mapping population of approximately 120 plants has been created by a cross between
Opuntia accession TAM 1281 and Opuntia accession TAM
1250. Copies of this population are available in California,
Georgia, and Argentina, and these plants will be used to
create a genetic map of cacti with an average spacing of
5 cM between markers. The sequences identified will be
used as RFLP probes to develop this map. Because of the
morphological variability between the parents, and based
on initial RAPD data, genetic polymorphism between the
parents may be frequently encountered. This map will be
publicly available, as will the clones from the mapping
population, allowing other researchers to use and extend
the map. Once an initial genetic map of cacti is created,
morphological markers will be placed on the map. This
will provide breeders a working framework and will also
help characterize the interesting phenotypes associated
with cacti.
Future Directions
Speeding the development of molecular tools for cacti
using other well-studied plant genomes will provide workers interested in the molecular biology of cacti with a set of
tools to accomplish their research goals. In addition, the localization of agronomic traits on the genetic map will allow
breeders to use molecular techniques in their programs.
However, the work described here is only an initial step toward the development and characterization of cacti at the
molecular level. Another important goal should be the development of transformation systems for cacti. Because
cacti may also be amenable to transformation using
Agrobacterium, development of these systems could supplement and extend the work being done with particle
bombardment. Research on tissue culture of cacti must be
extended to provide a rapid system for the regeneration of
transformed tissue into whole plants. Further work characterizing cactus germplasm using molecular markers can
help create a better understanding of the relationships between available accessions. Cultivars have been recognized
primarily by their morphological characteristics, which can
be misleading (Wang et al. 1998). Molecular markers
should be developed and utilized to evaluate germplasm resources, helping breeders determine the level of molecular
similarity between two cultivars in a cross.
Another area of potential research is the study of chromosomal changes underlying the variable polyploid nature
of the cactus genome. This has practical interest, because
chromosomal imbalance associated with polyploidy may
lead to partial seed set in O. ficus-indica (Nerd and Mizrahi
1994). Characterizing how polyploidy affects seed set may
lead to the development of techniques to produce varieties
with fewer seeds. In addition, physical maps of the cactus
genome should be developed to allow researchers to identify and sequence genes of interest in cacti more easily.
These tools should provide insight into the molecular basis
Breeding and Biotechnology
267
for many of the interesting phenotypes of cacti. The molecular characterization of these phenotypes will not only
be intriguing within the framework of understanding cactus and plant morphology, but may also be potentially useful for the improvement of other crop species.
Conclusions
The Cactaceae exhibit many unique phenotypes, making
it an ideal family for study by plant breeders and molecular biologists. This chapter has focused on the improvement of cactus germplasm via plant breeding and molecular techniques. Many modern varieties of cactus pear are
the products of long-term, informal, yet effective selection
by growers from plants in family gardens. These cultivars
were selected in Mexico or were obtained from Mexican
germplasm and adapted to other countries. More formal
cactus pear breeding was attempted in the early 20th century in California and again during the 1970s in Mexico.
Only recently have many of the advances and more formal
techniques been utilized by research groups around the
world. The molecular work done on cacti can benefit from
biotechnological work on other species. Increased work in
both breeding and biotechnology of cacti should provide
significant insights into the processes that contribute to
their unique phenotypes. Using these tools and techniques
to accomplish the major objectives outlined in this chapter will improve cactus cultivars available to both farmers
and consumers. Ultimately, breeding and biotechnology
will play an important role in increasing the understanding and usefulness of cacti.
Acknowledgments
B.C. thanks Peter Felker for useful discussions and the
Howard Hughes Medical Institute for a fellowship.
Research of A.H.P has been funded by the International
Arid Lands Consortium and the USDA Foreign Agriculture Service.
Literature Cited
Aguilar, B. A. 1987. Efecto de la aplicacion de acido giberelico (GA3) y urea en el fruto del nopal (Opuntia amychlaea Tenore). Master’s Thesis, Colegio de Postgraduados, Chapingo, Mexico.
Barbera, G. 1995. History, Economic and Agro-ecological
Importance. In Agroecology, Cultivation and Uses of
Cactus Pear (G. Barbera, P. Inglese, and E. PimientaBarrios, eds.). FAO Plant Production and Protection
Paper 132. FAO, Rome. Pp. 1–11.
Barbera, G., F. Carimi, and P. Inglese. 1991. The re-
268
Chapman, Mondragon, Bunch, and Paterson
flowering of prickly pear (Opuntia ficus-indica (L.)
Miller): Influence on removal time and cladode load
on yield and fruit ripening. Journal of the American
Society for Horticultural Science 5: 77–80.
Barbera, G., F. Carimi, and P. Inglese. 1992. Past and present pole of the Indian-fig prickly pear (Opuntia ficusindica (L.) Miller, Cactaceae) in the agriculture of
Sicily. Economic Botany 46: 10–20.
Barbera, G., P. Inglese, and T. La Mantia. 1994. Influence
of seed content on some characteristics of the fruit of
cactus pear (Opuntia ficus-indica Mill.). Scientia
Horticulturae 58: 161–165.
Bunch, R. 1997. Update on cactus pear breeding and new
products at D’Arrigo Bros. Journal of the Professional
Association for Cactus Development 2: 60–70.
Cantwell, M. 1991. Quality and postharvest physiology of
“nopalitos” and “tunas.” In Proceedings of the Second
Annual Texas Prickly Pear Conference. McAllen, Texas.
Pp. 50–66.
Chessa, I., G. Nieddu, P. Serra, P. Inglese, and T. L.
Mantia. 1997. Isozyme characterizaton of Opuntia
species and varieties from Italian germplasm. Acta
Horticulturae 438: 45–52.
Corrales-Garcia, J., J. Andrade-Rodriguez, and E. BernabeCruz. 1997. Response of six cultivars of tuna fruits to
cold storage. Journal of the Professional Association for
Cactus Development 2: 160–168.
Cui, M., P. M. Miller, and P. S. Nobel. 1993. CO2 exchange
and growth of the Crassulacean acid metabolism plant
Opuntia ficus-indica under elevated CO2 in open-top
chambers. Plant Physiology 103: 519–524.
Cushman, J. C., and H. J. Bohnert. 1997. Molecular genetics of Crassulacean acid metabolism. Plant
Physiology 113: 667–676.
Dreyer, P. 1985. A Gardener Touched with Genius: The Life of
Luther Burbank. University of California Press, Berkeley.
Escobar, H. A., V. M. Villalobos, and A. Villegas. 1986.
Opuntia micropropagation by axillary proliferation.
Plant Cell, Tissue and Organ Culture 7: 269–277.
Felker, P. 1995a. Forage and fodder production and utilization. In Agroecology, Cultivation and Uses of Cactus
Pear (G. Barbera, P. Inglese, and E. Pimienta-Barrios,
eds.). FAO Plant Production and Protection Paper 132.
FAO, Rome. Pp. 144–154.
Felker, P. 1995b. A review of cactus pear development in the
United States. In Memorias del VI Congreso Nacional y
IV Internacional sobre el Conocimiento y Aprovechamiento del Nopal. Guadalajara, Mexico. Pp. 217–221.
Janick and J. E. Simon, eds.). ASHS Press, Alexandria,
Virgina. Pp. 446–450.
Flores, V. C. 1995. “Nopalitos” production and processing.
In Agroecology, Cultivation and Uses of Cactus Pear (G.
Barbera, P. Inglese, and E. Pimienta-Barrios, eds.).
FAO Plant Production and Protection Paper 132. FAO,
Rome. Pp. 92–99.
Mondragon, J. C., and E. Pimienta. 1995. Propagation. In
Agroecology, Cultivation and Uses of Cactus Pear (G.
Barbera, P. Inglese, and E. Pimienta-Barrios, eds.).
FAO Plant Production and Protection Paper 132. FAO,
Rome. Pp. 64–70.
Gil, G. S., and A. Espinosa. 1980. Desarrollo de frutos de
tuna (Opuntia ficus-indica Mill.) con aplicación
prefloral de giberellina y auxina. Ciencia e Investigación
Agraria 7: 141–147.
Muratalla, L. A., P. F. Barrientos, and A. J. Rodriguez.
1990. Germinación de semilla de nopal (Opuntia amychlaea T. cv. ‘V5’ y O. ficus-indica cvs. ‘V1’ y ‘F1’). In
Memorias de la IV Reunion Nacional sobre el Conocimiento y Aprovechamiento del Nopal. Zacatecas, Mexico.
P. 19.
Granata, G., and A. Sidoti. 1997. Appearance of Alternaria
Golden Spot on cactus pear in Italy. Acta Horticulturae
438: 129–130.
Grant, V., A. K. Grant, and D. P. Hurd. 1979. Pollination
of Opuntia lindheimeri and related species. Plant
Systematics and Evolution 132: 313–320.
Haage, W. 1963. Cacti and Succulents: A Practical
Handbook. Simon & Schuster, New York.
Hillig, K. W., and F. A. Iezzoni. 1988. Multivariate analysis of a sour cherry germplasm collection. Journal of the
American Society for Horticultural Science 113: 928–934.
Hoffman, W. 1995. Ethnobotany. In Agroecology, Cultivation and Uses of Cactus Pear. (G. Barbera, P. Inglese,
and E. Pimienta-Barrios, eds.). FAO Plant Production
and Protection Paper 132. FAO, Rome. Pp. 12–19.
Loik, M. E., and P. S. Nobel. 1991. Water relations and
muccoploysaccharide increases for a winter hardy cactus during acclimation to subzero temperatures.
Oecologia 88: 340–346.
Loik, M. E., and P. S. Nobel. 1993. Freezing tolerance and
water relations of Opuntia fragilis from Canada and
United States. Ecology 74: 1722–1732.
Mondragon, J. C. 1999. Preliminary genetic studies on cactus pear (Opuntia spp. Cactaceae) germplasm from
Central Mexico. Ph.D. Thesis, Purdue University,
West Lafayette, Indiana.
Mondragon, J. C., and B. B. Bordelon. 1996. Cactus pear
(Opuntia spp. Cactaceae) breeding for fruit production. Journal of the Professional Association for Cactus
Development 1: 19–35.
Mondragon, J. C., and G. S. Perez. 1993. ‘Reyna’ (Syn.
Alfajayucan) is the leading cactus pear cultivar in central Mexico. Fruit Varieties Journal 48: 134–136.
Mondragon, J. C., and S. G. Perez. 1996. Native cultivars
of cactus pear in Mexico. In Progress in New Crops ( J.
Nerd, A., and Y. Mizrahi. 1994. Toward seedless prickly
pear. In Proceedings of the Fifth Annual Texas Prickly
Pear Council (P. Felker and J. R. Moss, eds.), Kingsville, Texas. Pp. 5–6.
Nerd, A., and Y. Mizrahi. 1995. Effect of low winter temperatures on bud break in Opuntia ficus-indica.
Advances in Horticultural Science 9: 1–4.
Nerd, A., and Y. Mizrahi. 1997. Reproductive biology of
cactus fruit crops. Horticultural Reviews 18: 321–346.
Nobel, P. S. 1988. Environmental Biology of Agaves and
Cacti. Cambridge University Press, New York.
Nobel, P. S. 1991a. Environmental productivity indices and
productivity for Opuntia ficus-indica under current and
elevated atmospheric CO2 levels. Plant, Cell and
Environment 14: 637–646.
Nobel, P. S. 1991b. Tansley Reveiw No. 32. Achievable productivities of certain CAM plants: basis for high values
compared with C3 and C4 plants. New Phytologist 119:
183–205.
Nobel, P. S. 1994. Remarkable Agaves and Cacti. Oxford
University Press, New York.
Nobel, P. S., and M. Castañeda. 1998. Seasonal, light and
temperature influences on organ initiation for unrooted cladodes of the prickly pear cactus Opuntia ficusindica. Journal of the American Society for Horticultural
Science 123: 47–51.
Nobel, P. S., and A. A. Israel. 1994. Cladode development,
environmental responses of CO2 uptake, and productivity for Opuntia ficus-indica under elevated CO2.
Journal of Experimental Botany 45: 295–303.
Ortiz, H. Y. 1988. Efecto del acido giberelico y auxinas en
el fruto del nopal tunero (Opuntia amychlae T.).
Master’s Thesis, Colegio de Postgraduados, Chapingo,
Mexico.
Breeding and Biotechnology
269
Parish, J., and P. Felker. 1995. Evaluation of fruit quality
and production of cold-hardy Opuntia fruit clones. In
Professional Association for Cactus Development First
Annual Conference (P. Felker and J. Moss, eds.),
Kingsville, Texas. Pp. 12a–e.
Saenz, C., and E. Costell. 1990. Rheology of prickly pear
(Opuntia ficus-indica) concentrated juices. In Engineering and Food, Vol. 1 (W. E. L. Spies and H.
Schubert, eds.). Elsevier Applied Science, London. Pp.
133–137.
Paterson, A. H. 1995. Molecular dissection of quantitative
traits: progress and prospects. Genome Research 5: 321–
333.
Sanderson, C. K., H. Yunn-Shy, C. W. Martin, and B. C.
Reed. 1986. Effect of photoperiod and growth regulators on growth of three cacti. HortScience 21: 1381–1382.
Paterson, A. H., T.-H. Lan, K. P. Reischmann, C. Chang,
Y.-R. Lin, S.-C. Liu, M. D. Burow, S. P. Kowalski,
C. S. Katsar, T. A. DelMonte, K. A. Feldmann, K. F.
Schertz, and J. F. Wendel. 1996. Toward a unified genetic map of higher plants, transcending the monocotdicot divergence. Nature Genetics 14: 380–382.
Santacruz-Ruvalcaba, F., A. Gutiérrez-More, and B.
Rodríguez-Garay. 1998. Somatic embryogenesis in
some cactus and agave species. Journal of the Professional Association for Cactus Development 3: 15–26.
Paterson, A. H., S. D. Tanksley, and M. E. Sorrells. 1991.
DNA markers in plant improvement. Advances in
Agronomy 46: 39–90.
Pilbeam, J. 1987. Cacti for the Connoisseur: A Guide to
Growers and Collectors. Timber Press, Portland, Oregon.
Pimienta-Barrios, E. 1990. El Nopal Tunero. Universidad de
Guadalajara, Mexico.
Pimienta-Barrios, E.., and L. Mauricio. 1987. Variación en
los componentes del fruto maduro entre formas de
nopal (Opuntia spp.) tunero. Revista de Fitotecnia
Mexicana 12: 183–196.
Pimienta-Barrios, E., and U. A. Muñoz. 1995. Domesticación of opuntias and cultivated varieties. In Agroecology, Cultivation and Uses of Cactus Pear. (G.
Barbera, P. Inglese, and E. Pimienta-Barrios, eds.).
FAO Plant Production and Protection Paper 132. FAO,
Rome. Pp. 58–63.
Pinkava, D. J., M. A. Baker, B. D. Parfitt, M. W.
Mohlenbrock, and R. D. Worthington. 1985. Chromosome numbers in some cacti of western North
America—V, with nomenclatural changes. Systematic
Botany 10: 471–483.
Pinkava, D. J., B. D. Parfitt, M. A. Baker, and R. D.
Worthington. 1992. Chromosome numbers in some
cacti of western North America—VI, with nomenclatural changes. Madroño 39: 98–113.
Pizzeti, M. 1985. Guide to Cacti and Succulents. Simon and
Schuster, New York.
Saad, M., C. A. Degano, and J. Ochoa. 1998. Wet rot and
necrosis caused by bacteria in Opuntia ficus-indica
Mill. in Santiago del Estero, Argentina. Journal of the
Professional Association for Cactus Development 3: 60–
63.
270
Chapman, Mondragon, Bunch, and Paterson
Silos-Espino, H., E. R. Salazar, Q. R. Cruz, J. O. Castro,
and O. P. Lopez. 1999. Proteinas de reserva de importancia alimentaria del Nopal. In Memorias del VIII
Congreso Nacional y VI Internacional sobre el Conocimiento y Aprovechamiento del Nopal (R. Aguirre and
J. A. Reyes, eds.). San Luis Potosí, Mexico. Pp. 36–37.
Staub, J. E., F. C. Serquen, and M. Gupta. 1996. Genetic
markers, map construction and their application in
plant breeding. Horticultural Science 31: 729–740.
Sudzuki, H. F. 1995. Anatomy and Morphology. In
Agroecology, Cultivation and Uses of Cactus Pear. (G.
Barbera, P. Inglese, and E. Pimienta-Barrios, eds.).
FAO Plant Production and Protection Paper 132. FAO,
Rome. Pp. 28–35.
Thomashow, M. F. 1998. Role of cold-responsive genes in
plant freezing tolerance. Plant Physiology 118: 1–7.
Torres-Muñoz, L., and B. Rodríguez-Garay. 1996. Somatic
embryogenesis in the threatened cactus Turbinicarpus
pseudomarcrochele (Buxbaum and Backeberg). Journal
of the Professional Association for Cactus Development 1:
36–38.
Trujillo, A. S. 1986. Hibridización, aislamiento, y formas de
reproducción de Opuntia spp. Master’s Thesis, Colegio
de Postgraduados, Chapingo, Mexico.
Uchoa, A. F., P. A. S. Souza, R. M. L. Zárate, E. GomesFilho, and F. A. P. Campos. 1998. The isolation and
characterization of a reserve protein from the seeds of
Opuntia ficus-indica (Cactaceae). Brazilian Journal of
Medical and Biological Research 31: 757–761.
Uzun, I. 1997. Fruit and cladode isozymes in cactus pear.
Acta Horticulturae 438: 53–56.
Villalobos, A. V. 1995. Tissue Culture application of
Opuntia sp. micropropagation. In Agroecology Cultivation and Uses of Cactus Pear (G. Barbera, P. Inglese, and
E. Pimienta-Barrios, eds.). FAO Plant Production and
Protection Paper 132. FAO, Rome. Pp. 71–77.
Wang, X., P. Felker, M. D. Burow, and A. H. Paterson.
1998. Comparison of RAPD marker patterns to morphological and physiological data in the classification
of Opuntia accessions. Journal of the Professional
Association for Cactus Development 3: 3–14.
Wang, X., P. Felker, and A. Paterson. 1997. Environmental
influences on cactus pear fruit yield, quality and cold
hardiness and development of hybrids with improved
cold hardiness. Journal of the Professional Association for
Cactus Development 2: 48–59.
Wang, X., P. Felker, A. Paterson, Y. Mizrahi, A. Nerd, and
J. C. Mondragon. 1996. Cross-hybridization and seed
germination in Opuntia species. Journal of the Professional Association for Cactus Development 1: 49–60.
Weiss, J., A. Nerd, and Y. Mizrahi. 1993. Vegetative
parthenocarpy in the cactus pear Opuntia ficus-indica
(L.) Mill. Annals of Botany 72: 521–526.
Weniger, D. 1984. Cacti of Texas and Neighboring States: A
Field Guide. University of Texas, Austin.
Winicov, I. 1998. New molecular approaches to improving
salt tolerance in crop plants. Annals of Botany 82: 703–
710.
Zárate, R. M. L., F. A. P. Campos, and J. Landsmann.
1998. Establishment and transformation of callus and
cell suspension cultures of the prickly-pear (Opuntia
ficus-indica). Journal of the Professional Association for
Cactus Development 3: 27–33.
Zárate, R. M. L., L. F. A. Ponte, J. Landsmann, and
F. A. P. Campos. 1999a. Biolistic-mediated transient
gene expression in shoot apical meristems of the
prickly-pear (Opuntia ficus-indica). Brazilian Archives
of Biology and Technology 42: 78–86.
Zárate, R. M. L., L. F. A. Ponte, J. Landsmann, and
F. A. P. Campos. 1999b. Whole plant regneration from
the shoot apical meristem of Opuntia ficus-indica
(Cactaceae). Journal of Applied Botany 73: 83–85.
Zárate, R. M. L., C. Studart-Guimaráes, J. Landsmann,
and F. A. P. Campos. 1999c. Establishment of callus
and cell suspension cultures of Opuntia ficus-indica
Mill. Plant, Cell, Tissue and Organ Culture 58: 155–157.
Wessels, B. A. 1988. Spineless Prickly Pear. Perskor,
Johannesburg.
Breeding and Biotechnology
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INDEX
Adhesives, 148–151
Aztekium, 11, 44
Adventitious roots, 8, 43–46, 169, 187
Aztekium ritteri, 131
Agrobacterium tumefaciens, 245–246
Alcoholic beverages, 148–151, 158, 165
Backebergia militaris, 36
Algeria, 159, 200, 201, 202
Bacteria and bacterial infections, 52, 177, 244–247
Allozymes, 127–128
Bacterial spot, 245–247
Alternaria golden spot, 245, 247–248
Ants, 79, 97, 99, 240, 241
Barrel cacti, 10, 11, 17, 62, 64, 68, 83, 91, 143, 148–151. See also
individual species
Apical meristem: shoot, 30, 34, 35, 266; root, 42, 45–47, 49
Bats, 11, 77, 95, 102–103, 128, 154, 187
Apomixis, 83–86, 265, 266.
BCT clade, 9, 10, 12
Aposematic, 237, 239
Bees, 77–78, 95, 103, 191, 262
Archaeology, 143, 146, 151, 159, 190, 222
Betalains, 2, 222
Areoles, 3, 5, 34, 43, 75–76, 154, 262
Biodiversity, 91–92, 100, 103, 125–130, 136–138
Argentina, 1, 75, 168, 191, 201, 242–245, 262
Biological control, 235, 241, 243–244, 249, 250
Arid regions, 49, 75, 81–82, 109, 114, 126, 158, 167, 199–201,
204–205, 256, 258
Biomass productivity, 65–66, 170, 172, 201, 256
Ariocarpus fissuratus, 24–25, 67
Biotechnology, 255–257, 265–268
Armillaria, 245–246
Birds, 93, 97–99, 102, 109, 110, 113, 116–119
Artificial selection, 144–145, 152, 154, 159
Bison, 84
Atacama Desert, 70
Bolivia, 201, 236, 244, 249
Atmospheric CO2, 63, 256
Boron, 52
Australia, 84
Brazil, 191, 200, 201, 236, 243
Axillary buds, 34, 75
Breeders and breeding, 127, 129, 256–265, 267–268
Aztec, 156, 222
Breeding system evolution, 127–128
Biomechanics, 36, 69
273
Brine (for nopalitos), 215–217
Burbank, Luther, 258, 260, 261
CITES (Convention on International Trade in Endangered
Species of Wild Fauna and Flora), 131–136, 139
Burro, 119
Clade, 2, 5, 10, 12, 14, 17
C3, 23, 26, 58, 60, 62, 63, 65, 68–69, 94
C4, 26, 60, 62, 65, 68–69
Cacteae, 11
Cactoblastis cactorum, 84, 238–245, 250
Cactoideae, 5, 6, 8, 10, 16–17, 24, 26–27, 29–30, 33–34, 59
CactusNet, 179, 258
Cactus pear, 163–179, 236, 241, 244–251, 257–265. See also
Cladodes; Fruits; Opuntia ficus-indica
Calcium (Ca), 202, 203, 207, 212
Calcium oxalate, 5, 7, 16, 17, 29, 42–43, 115, 202, 212
Calymmanthium, 8, 10
CAM. See Crassulacean acid metabolism (CAM)
Canada, 68, 84, 134, 165, 256, 257
Canary Islands, 157, 168, 228, 241
Candies, 218
Carbohydrate source/sink, 63
Carmine and carminic acid, 212, 222–224, 228, 240, 250
Carnegiea gigantea, 35, 36, 49, 58–59, 67, 69, 79, 94, 96, 98, 101
Caryophyllales, 2–3, 16
Casparian strip, 42
Cattle, 120, 147, 151, 200, 202–204, 206, 257
Cell cycle, 46
Cellulose, 202, 204–205, 207, 219. See also Cell walls
Cell walls, 26, 29, 30, 32, 37, 68
Cephalia, 32, 34–36
Cladodes: abiotic responses, 63; anatomy and morphology, 37;
conservation and diffusion, 156–157; evolution and molecular
aspects, 13, 264–265; human consumption, 169, 171, 212–213,
219–221; nonvertebrate consumption and interactions, 237,
242, 247–249; orientation, 58, 62, 179; vertebrate consumption, 109–115, 118, 120, 199–207, 203–207
CO2 uptake, 58–66
Coccidae, 225
Cochineal, 147, 156, 157, 170, 212, 222–229. See also Dactylopius
Cold, resistance to, 83, 256, 258–260, 266, 267
Collared peccary, 112–115, 118, 120
Collecting and collectors, 129, 131, 133, 135–136, 139, 151, 155
Colombia, 186, 193, 201
Color-break, 173, 189, 191
Columnar cacti: abiotic responses, 66, 68; anatomy and morphology, 29, 34, 37; biotic responses, 77–78, 82, 91–92, 95–
96, 98, 101, 103; conservation and diffusion, 125, 143–144,
146–155; evolution, 8, 10–13, 17; human consumption, 189–
195, 264. See also individual species
Companion cells, 32
Consensus tree, 4, 9, 15
Conservation, 126, 129–139, 201–202
Convergent evolution, 7
Coreidae, 237, 241
Cortex, 26–27, 30, 37, 42–43
Cortical bundles, 30
Coryphantha, 2, 67, 93–94
Cosmetics, uses of cacti in, 221, 222, 223
Cephalocereus columna-trajani, 25
Crassulacean acid metabolism (CAM), 1, 16, 23, 26, 58–59, 62,
70, 75, 194, 236, 239, 240, 256, 267
Cephalocereus senilis, 29
Crown gall, 245–246
Cerambycidae, 236–238, 250
Curculionidae, 236–237, 241, 250
Ceratitis capitata, 169, 237, 241–243
Cuticle, 24, 37, 61, 69, 75, 178, 187, 236
Cereus jamacaru, 186, 241
Cylindropuntia, 14, 236, 238
Cereus peruvianus, 186, 191–192, 194, 262
Cylindropuntias, 42, 78, 84
Cereus validus, 59, 62, 69
274
Chihuahuan Desert, 91, 95, 100–103, 145
Dactylopiidae, 236–238, 240, 241, 250
Chile, 1, 75, 137, 165, 201, 203, 228, 242, 244, 245, 262
Dactylopius, 224, 226, 238, 240, 242
Chlorenchyma, 26, 52, 58, 62–63, 68
Dactylopius coccus, 222, 224–227, 229, 235, 238, 240, 250
Chlorophyll, 26, 30, 36, 264
Dactylopius opuntiae, 224, 238, 240–244
Chloroplast DNA (cpDNA), 2–3, 8, 11
Deer, 112, 114, 120
Christmas cactus, 129
Desert. See specific deserts
Chromosomes, 80, 86, 168, 186, 225, 227, 256, 259, 266, 267
Desertification, 158–159, 200, 201
Index
Diarrhea, 115
Ferocactus acanthodes, 27, 49, 51–53, 58–59, 63–65, 67, 69, 94,
218
Diaspis echinocacti, 236, 241–243, 250
Ferocactus peninsulae, 45–46, 48
Dichogamy, 79
Ferocactus wislizenii, 57, 67
Dietary fiber, for humans, 218–219
Fiber, 202, 203, 207, 212, 218–219, 228
Digestibility, for animals, 203–207
Firewood, 125, 148–151
Dioecy, 80, 128
Flower buds, 151
Diptera, 237
Diseases, 236, 244–251, 256, 259
Flowers, 3, 7, 36, 76–82, 86, 95, 99; and animals/humans,
110–112, 116, 118, 125, 128–129, 147–151, 175–176, 249,
262–263
DNA, 2–3, 8, 11, 266, 267
Fodder, 147–151, 199–202, 206–207, 260, 261
Domestication, 143–155, 256, 257
Foot rot, 245, 247–248
Drought, 27, 29, 44, 48, 58, 61, 68, 81, 83, 200–201, 206, 256,
258, 259
Forage, 160, 199–201, 204, 258, 265
Diabetes mellitus, 219, 221, 229
Druses, 5, 7, 26, 29, 32
Freezing damage, 58, 67, 83
Frost and frost tolerance, 83, 256, 258–260, 266, 267
Echinocereus sciurus, 27–28
Fruits, 3, 63–64, 82, 83, 97; development, manipulation, and
production, 171–172, 174–177, 189, 191, 259; harvest, marketing, and selling arrangements, 166–167, 173–174, 179, 189,
195, 249, 256–257, 259–260; human consumption, 146–155,
158, 163–179, 185–195, 261, 265; nonhuman consumption,
109–112, 117, 242, 250; quality/sugars, 152, 173, 176–178,
189
Echinopsis chamaecereus, 130
Fundamental tissue, 26
Ecuador, 186, 193
Fungi and fungal infections, 176, 236, 244–250
Eggstick, 239
Fungicides, 177, 179, 246, 247
Easter cactus, 128–129
Echinocactus platyacanthus, 93
Echinocereus engelmannii, 93–94, 218
Echinocereus schmollii, 31
Emasculation (flower), 262–264
Embolisms, 33, 51
Galápagos Islands, 79, 113, 115–120, 137
Endangered species, 131–137, 151, 264
Genetic diversity, 126–128, 138
Endodermis, 42–43
Geophytes, 3, 35, 44–45
Environmental Productivity Index (EPI), 64–66
Germination, 82–83, 92–94, 97, 99–100, 264
Enzyme, 128–129. See also PEPCase; Rubisco
Germplasm, 128, 138–139, 168, 187, 255–256, 258–262, 266–
268
Epicuticular wax, 178
Epidermis, 25, 28, 29, 37, 43, 237, 246
Gibberellic acid (GA), 82, 92, 175, 177, 257, 264
Epiphyllum, 59
Glochids, 113, 160, 165, 174, 176–179, 185, 257
Epiphyllum phyllanthus, 43, 50–51
Glucose, 219, 221
Epiphytes, 1, 3, 7, 8, 10, 17, 41, 43, 60–61, 70, 75, 78
Goats, 204–206
Erosion (soil), 151, 158, 201–202
Grafting, 264–265
Erwina, 246
Gray mold, 245, 249–250
Escontria chiotilla, 28, 102
Growth rings, 33–34, 37
Espostoa melanostele, 36
Ethnobotany, 147–151
Hailstorms, 167, 244, 246
Evolution, 7, 14–17, 23, 75, 78, 80, 86, 97, 267; and animals/
humans, 117, 119–120, 126, 128, 144, 236, 244
Hallucinations, 125, 134
Harrisia, 12, 16, 235
Hatiora, 128–129
Fences, 148–151, 158
Hawkmoths, 7, 95, 103, 187
Ferocactus, 93
Heavy metals, 52
Index
275
Hedges, 148–151, 158
Lophophora williamsii, 134–135, 147
Hemiepiphytes, 34, 60
Low-temperature hardening, 66
Houses, 148–151, 218–219
Human diets, 143, 146–151, 218–219, 228
Maihuenia, 5, 7, 27, 59
Hummingbirds, 7, 10, 11, 77, 95, 103
Maihuenioideae, 5, 6, 58
Hybridization, 69, 129, 257–259, 261, 265–267
Mammals, 93, 98, 109, 111–112, 116, 119
Hydraulic conductivity (LP), 50–51
Mammillaria, 11, 29, 77, 94
Hylocereeae, 10, 17
Mammillaria dioica, 52, 59, 67
Hylocereus, 8, 10, 12, 147
Mammillaria magnimamma, 35
Hylocereus costaricensis, 186, 187, 193, 262
Markets and marketing: fruits, 166–167, 176, 178, 192–194, 256–
257, 259–260; nopalitos, 215–217
Hylocereus polyrhizus, 186–189, 193, 262
Hylocereus undatus, 59–61, 63, 75, 147, 155, 159, 185–189, 192–193,
194, 262
Marmalades, 165, 212, 215, 217–218
Hypodermis, 8, 16, 24–27, 29, 42–43
Mediterranean fruit fly, 169, 237, 241–243
Medicines, 147–151, 156, 219–221, 223
Mediterranean region, 157–160, 163, 242–243, 249
Idioblasts, 27, 29
Medullary bundles/rays, 27, 30
Iguanas, 110, 112, 115, 118
Melocactus peruvianus, 36
Indian figs, 155, 157
Mescaline, 7, 134
Inferior ovary, 75
Mesoamerica, 143–147, 151, 156, 159, 221, 258
Insects, 93, 98, 117, 235–244, 256, 262
Metapopulation, 101
Intercellular air spaces, 26
Metaxylem, 42
IOS (International Organization for Succulent Plant Study), 6
Mexico: cactus biotic responses, 75, 83–84, 91, 101, 103; conservation, diffusion, and evolution, 11, 133, 134, 137, 144–151, 156;
human cactus consumption, 163, 165, 168, 169, 176, 178, 189,
190, 192, 193, 212, 214–217, 220–222, 256–262, 264, 265, 268;
nonhuman cactus consumption and interactions, 114, 119,
200, 201, 224, 226–228, 242–245, 249
Irrigation, 172–173, 176, 187, 191, 194, 260
Israel, 159, 168, 169, 174, 186–189, 191–193, 201, 258–260
Italy, 165–169, 174, 176, 201, 244, 245, 258, 259, 261, 264. See also
Sicily
Microorganisms, 212, 214
Jams, 147, 165, 217
Mites, 226, 237, 240
Mojave Desert, 137
LP, 50–51
Mold, 245, 249–250
Larvae (Cactoblastis), 239, 240
Molecular biology, 6, 265–268
Lateral roots, 43, 45–46, 48, 51, 264
Moths, 7, 78, 95
Laticifers, 26, 29
Leaves, 3, 5, 13, 16, 24, 26, 29, 34
Mucilage, 27, 50, 64, 151, 155, 214, 218, 219, 236, 239, 240, 263,
266
Lepidoptera, 242
Mucilage cells, 8, 16, 25–29, 32, 42, 44
Libriform fibers, 30, 32, 37
Mycorrhizae, 52
Life cycle, 97–100, 103, 225
Myrtillocactus geometrizans, 93
Light, 58, 62, 99
Myrtillocactus schenckii, 25
Light responses, 58, 62, 64, 170. See also PPF Index (EPI)
276
Lignin and lignification, 36–37, 202, 219
Native American Church, 133–134
Livestock. See specific animals
Nectar and nectary, 76–79, 95, 110–111, 116, 118, 128
Lizards, 98, 110, 117
Negev Desert, 168, 191
Lophocereus schottii, 94, 96
Neobuxbaumia macrocephala, 96
Lophophora, 3, 44–45
Neobuxbaumia mezcalaensis, 96, 159
Index
Neobuxbaumia tetetzo, 93, 95–96, 98–100, 102, 159
Opuntia versicolor, 57
Neoteny, 6
Opuntia violacea, 112
Net CO2 uptake. See CO2 uptake; Fruits; Photosynthesis
Opuntioideae, 5–7, 13–16, 24, 26–27, 33–34, 58–59, 236, 238
Nicaragua, 186, 193
Orchard design and management (O. ficus-indica), 169, 174,
178
Nitrogen (N), 52, 93, 172, 175, 202, 206, 207
Nocturnal acid accumulation, 62. See also CO2 uptake;
Crassulacean acid metabolism (CAM)
Ornamentals, 125, 128–129, 148–151, 156, 159
Nopal, 92, 113, 117
Outcrossing, 79–80, 86, 128
Nopalea, 29, 212, 257
Overgrazing, 199, 205–206
Nopalea cochenillifera, 213, 224, 226
Ovule, 77, 80, 82, 119
Nopaleras, 84, 85, 92, 101, 113, 120
Oxalates, 5, 7, 16, 17, 29, 42–43, 115, 202, 212, 236
Osmotic pressure, 58, 63, 66, 68, 69
Nopal flour, 219
Nopalito processing, 214–218
Pachycereeae, 10, 13, 16
Nopalitos, 154, 157, 171, 212–218, 228, 257–258, 260, 261
Pachycereus pringlei, 31, 37, 45–46, 52, 59, 69, 76, 93, 96, 99–103,
128
Nopal products, 215–218
Notocacteae, 11
Nurse plants, 52–53, 92–95, 97, 99, 101
Nutrients, 51–52, 82, 99, 115, 117, 171–172
Nutrition (animal), 117, 202–207
Nymph (cochineal), 225
Pachycereus schottii, 127
Packrats, 111–116, 120
Pads. See Cladodes
Parasitoids, 226, 239–240
Parthenocarpy, 257
Pathogens, 176–177
Obesity, 219, 221, 229
Opuntia, 12, 143–147, 155; anatomy and morphology, 26, 34;
human consumption, 167, 220–221, 255, 260, 266;
nonhuman consumption, 112, 203, 236, 239, 241–244, 246,
249–250; responses, 68, 83–86
Opuntia acanthocarpa, 59, 67, 218
Opuntia basilaris, 27, 59, 67, 218
Opuntia echios, 35, 138
Opuntia engelmannii, 203
Opuntia ficus-indica: abiotic responses, 59, 61–63, 66, 67, 69;
anatomy and morphology, 37, 43, 45–46, 49, 51–53; biotic
responses, 59, 61–63, 66, 67, 69, 75, 80–81, 85; conservation
and diffusion, 128, 155–157; human consumption, 163–179,
212–215, 218, 256–257, 259–260, 263–264, 266–267; nonhuman consumption and interactions, 114, 199–207, 224,
236–241, 244, 247
Peccary, 112–115, 118, 120
Penicillium, 250
Peniocereus striatus, 31
PEPCase, 58, 60, 63
Pereskia, 5, 7, 16, 27, 30, 59
Pereskia grandifolia, 35, 59
Pereskioideae, 5, 6, 26, 27, 33, 58–59, 236, 238
Pereskiopsis, 29, 59
Perianth, 77
Pericycle, 42–43, 48
Periderm, 26, 42, 51
Peru, 75, 201, 222, 223, 226, 228, 244, 249
Pests, 165, 169, 193, 222, 224–225, 229, 237–244, 250, 259
Petals, 110–112
Opuntia fragilis, 66, 67, 84, 85, 126
Peyote, 134–135, 147
Opuntia humifusa, 59–60, 63, 66–68, 70, 126, 127
Phellem/Phelloderm, 48
Opuntia leucotricha, 62
Philippines, 186
Opuntia littoralis, 69
Phloem, 30–32, 42, 63
Opuntia polyacantha, 59, 67, 202, 204
Phoma sorghina, 245, 249–250
Opuntia rastrera, 85, 98
Phosphoenol pyruvate carboxylase (PEPCase), 58, 60, 63
Opuntia robusta, 147, 203, 212, 214, 259
Phosphorus (P), 52, 172, 202, 203, 206, 207, 212
Opuntia streptacantha, 85, 98, 259
Photoblastic, 92
Opuntia stricta, 59, 84
Photoinhibition, 62
Index
277
Photosynthetic enzymes. See PEPCase; Rubisco
Rain roots, 48
Photosynthetic photon flux (PPF), 60–62, 93
Random amplified polymorphic DNA (RAPD), 259, 266
Photosynthesis, 36, 57–58, 66. See also C3; C4; Crassulacean acid
metabolism (CAM); CO2 uptake
Rathbunia alamosensis, 35
Phototropism, 58, 62
Receptacle, 76–77
Phytochrome, 82
Reptiles, 98, 102, 110, 112, 116–117
Phytophthora cactorum, 245, 247–249
Respiration, 52, 66, 212–213
Phytoplasmas, 244–245, 250
Rhipsalideae, 10
Pitahayas, 147, 185–186, 194–195
Rhipsalis, 1, 30, 59, 129
Pitayas, 147, 185–186, 194–195
Rhipsalis baccifera, 43, 51
Pith, 26–27, 29, 30, 32
Rhipsalis pentaptera, 129
Placentation, 3
Rhizosheaths, 50–53
Planting, of crops, 169, 174–175, 188, 194
Ribs, 5
Plant spacing (O. ficus-indica), 170–171, 174
Ribulosebisphosphate carboxylase/oxygenase (Rubisco), 58–60,
63
Platyopuntia, 238
Platyopuntias, 12, 14, 155–160, 221, 264; anatomy and morphology, 27, 34, 37, 42; nonhuman consumption, 109–119, 199–
207, 224, 227; responses, 58, 62–67, 69, 84
Pleistocene, 103, 112, 118, 120
Polaskia chende, 25
Pollen, 5, 77–81, 95, 110–111, 116–118, 128, 186, 262, 266
Pollination and pollinators, 7, 10, 77–82, 95–96, 99, 103, 118–
119, 128, 187, 191, 257, 262–264
Polyploidy, 80, 85, 186, 256–257, 265, 267–268
Population and population dynamics, 98–100, 103
Postharvest: fruits (O. ficus-indica), 173, 176–178, 257; stems (O.
ficus-indica), 213, 215
Rays, 32, 34, 37
Ripening, of fruit, 173–174, 176, 189, 259
Rocks, 41, 49
Rodents, 98, 99, 102, 111, 113, 117, 118, 120
Root apical meristem, 42, 45–47, 49,
Root cap, 46
Root development and growth, 45–46, 169
Root hairs, 42, 46–47, 50
Root hydraulic conductivity, 50–51
Root respiration, 52
Roots and rooting system, 42–45, 69, 202
Root/shoot ratio, 49, 51, 52
PPF Index (EPI), 64–65
Root-soil air gaps, 51
Productivity (biomass), 65–66, 170, 172, 201, 256
Root spurs, 46
Propagation, 134–135, 145, 159, 169
Rot, 245–247
Protandry, 262
Rubisco, 58–60, 63, 267
Protein, 117, 202–204, 207, 212, 266
Ruminants, 202–207
Protozoa, 204–205
Pruning (O. ficus-indica), 170–171, 175–176
Salinity, 62–63, 69, 168, 172, 191
Pterocactus tuberosus, 35
Sap suckers, 238, 240, 242
Pubescence, 62
Schlumbergera, 128–129
Pulp (fruit), 152, 176, 189, 193, 257
Sclerids, 29, 31–32
Pyralidae, 236–241, 250
Scozzolatura, 158, 164–166, 174–176
Seed coat and seed testa, 82
Quiabentia, 59
Seed dispersal and germination, 82–83, 92–94, 97, 99–100, 264
Seedlings, 50, 53, 83, 92–94, 99–100, 264, 266
Rabbits, 111–113, 115, 119
Radicle, 42–43, 46–47, 50
Rain, 60, 201, 240
278
Index
Seeds, 42, 147–151, 153–154; evolutionary/molecular aspects, 2, 5,
7, 14, 257–258, 264, 266, 267; human consumption, 165, 176,
179, 186, 188; nonhuman consumption, 109–111; responses,
82–83, 85, 92–93, 99
Selenicereus, 8, 10
Taproots, 44–45, 49
Selenicereus megalanthus, 186, 187, 189, 193, 262
Tehuacán Valley, 46, 91, 94, 96, 100–101, 103, 146–147, 151–
154
Semiarid regions, 66, 75, 84, 117, 126, 168, 199–201, 204, 239,
256, 258
Temperature, 60, 66–68. See also CO2 uptake
Sheep, 200–207, 257
Temperature Index (EPI), 64–65
Shelf life: fruits, 176, 178, 179, 189, 190, 192, 194; stems, 213–
214
Threatened species, 131–137, 139
Sicily, 157–158, 164, 166–167, 170, 260, 261
Tortoises, 110, 112, 115, 118, 119
Sieve tubes, 32
Tracheids, 5, 6
Silica bodies and grains, 28, 29
Transpiration, 24, 60, 75
Sink, carbohydrate, 63, 171, 175
Tree, consensus, 4, 9, 15
Sodium (Na), 62, 69, 202, 203, 206, 207, 212
Trichocereus, 67, 95
Soft rot, 245–247
Trichomes, 7, 35–36
Soil, 51, 53, 69, 92–93, 99, 103, 249
Trioecy, 80, 128
Soil erosion, 151, 158
Tubercles, 29, 257
Soil water content, 169
Tuberous roots, 44
Solar irradiation, 61
Tunas, 155, 157
Sonoran Desert, 46, 49, 52, 65, 79, 92, 94, 97, 100–102, 145, 174,
218
Tunisia, 159, 200–203
Source/sink, 63, 171
South Africa, 164, 168, 169, 201, 239, 241, 243, 244, 249, 258–262
Spacing, plant (O. ficus-indica), 170–171, 174
Tissue culture, 266, 267
United States: conservation, 132, 134, 137; human cactus consumption, 165, 168, 169, 175, 186, 193, 256, 257, 259–261;
nonhuman cactus consumption, 201, 203, 222, 224, 242
Spines, 3, 10, 34–36, 62, 115, 118, 165, 226, 257, 258
Stamens, 76–79, 263
Vascular cambium, 32, 34
Starch, 27, 30, 42, 44, 64, 82, 189
Vascular cylinder/tissue, 30, 42. See also Phloem; Xylem
Stele, 30, 32
Vascular (wide-band) tracheids, 30–34, 37
Stem area index (SAI), 65–66, 170–171
Stenocereus, 12, 84, 102, 189, 193
Vegetable (nopalitos), 154, 157, 171, 212–218, 228, 257–258, 260,
261
Stenocereus griseus, 186
Vertebrates (wild), 109–120. See also specific animals
Stenocereus gummosus, 28, 45–48, 50, 52, 98
Vessels, 30, 32–33, 42–43
Stenocereus pruinosus, 152, 159
Vietnam, 186, 187, 192
Stenocereus queretaroensis, 59–60, 62–63, 67, 75, 81–82, 151–152,
159, 185–186, 190–191, 194
Vine cacti, 185–189, 192–195
Stenocereus stellatus, 151–154, 159, 186, 190–191
Vitamins, 176, 207
Viruses, 244–245, 250
Stenocereus thurberi, 52, 67, 93, 95–97, 128
Stigma, 76–77, 79–80, 119, 263
WANA (West Asia/North Africa) region, 199–207
Stomata, 24, 26, 58–61, 68, 75, 246
Water Index (EPI), 61, 64–65
Style, 76–77, 80, 119, 263
Water relations, 61, 205, 236
Sugars, 173, 189, 202, 205–207, 219, 246
Water storage capacity, 44, 81
Surface area–volume ratio, 8
Water stress, 27, 29, 44, 48, 58, 68, 113
Survival, 66–69
Water-use efficiency, 60–61, 172, 201
Wax (cuticle), 178
Taiwan, 186, 187–188, 192
Weberbauerocereus weberbaueri, 96, 127
Tannins, 31–32
West Asia/North Africa (WANA) region, 199–207
Index
279
Wide-band tracheids, 30–34, 37
Xoconoztles, 147, 154
Wilcoxia poselgeri, 28, 31
Xylem, 30, 32, 42–43, 69
Wild cochineal, 222, 226–227
Wind, 41, 69, 97, 158, 201, 202
Yeasts, 244–247
Wood, 32–34, 36–37, 69
Wounds/wound pathogens, 177, 245–246, 263
280
Index
Zoochory, 97
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