ISSN (print) 0093-4666
© 2015. Mycotaxon, Ltd.
ISSN (online) 2154-8889
MYCOTAXON
http://dx.doi.org/10.5248/130.11
Volume 130, pp. 11–15
January–March 2015
Additions to rust and chytrid pathogens of Turkey
Cumali Özaslan1*, Makbule Erdoğdu2,
Elşad Hüseyin2 & Zekiye Suludere3
Dicle University, Faculty of Agriculture, Department of Plant Protection, Diyarbakır-Turkey
Ahi Evran University, Faculty of Science and Literature, Department of Biology,
Kırşehir-Turkey
3
Gazi University, Faculty of Science, Department of Biology, Ankara-Turkey
*Correspondence to: cumali.ozaslan@dicle.edu.tr
1
2
Abstract — Uromyces bornmuelleri on Bongardia chrysogonum and Physoderma maculare
on Alisma plantago-aquatica have been recently discovered in Turkey. Morphological data
obtained by light and scanning electron microscopy of identified fungi are presented.
Key words — new records, Chytridiomycota, Basidiomycota
Introduction
The Pucciniaceae are the largest family within the Uredinales, parasitising
almost all major angiosperm orders, with some primarily herbaceous plant
families such as Asteraceae and Poaceae being most commonly infected (van
der Merwe et al. 2007). Uromyces (Link) Unger was raised to generic rank by
Unger (1832), and is typified by U. appendiculatus on Phaseolus. Seventy-four
species of Uromyces have been registered on 227 species of higher plants in
Turkey (Bahçecioğlu & Kabaktepe 2012).
All known representatives of the genus Physoderma Wallr.
(Blastocladiomycetes, Physodermataceae) are obligate parasites of vascular
plants (Olson et al. 1980). The generalized life cycle of Physoderma is composed
of two distinct and separate phases, and the ephemeral epibiotic sporangium
with an endobiotic rhizoidal system is confined to a single host cell (Sparrow
& Johns 1965). Physoderma maculare, the type species of the genus, on Alisma
confirms Clinton’s (1902) account of the epibiotic stage being produced from
resting zoospores. The latter on mature host tissue may also give rise to the
endobiotic stage, which bears resting spores. On seedlings, however, resting
zoospores produce only epibiotic sporangia (Sparrow 1964).
12 ... Özaslan & al.
Figs 1–7. Uromyces bornmuelleri. 1: Deformation of host plant. 2: Telia on leaf, in situ. 3: Teliospores.
4–6: Telia and teliospores (SEM). 7. Teliospores (SEM).
Materials & methods
Plant specimens infected with microfungi were collected from Adıyaman and
Siirt provinces, Turkey and prepared according to established herbarium techniques.
Host plants were identified using the Flora of Turkey and East Aegean Islands (Davis
1965–85). The fungal specimens were prepared from the host plants by obtaining thin
sections. Measurements were made from tissues mounted in 5% KOH or tap water.
Microscopical features were examined and microphotographs were made using a Leica
Uromyces and Physoderma spp. new for Turkey ... 13
DM E light microscope. Thirty spores were measured for each sample. The microfungi
were identified using relevant literature (De Toni 1888, Saccardo & Sydow 1902,
Gonzalez Fragoso 1918, Kuprevich & Ulijanishchev 1975, van der Merwe et al. 2007).
Species names follow Index Fungorum (2014). The examined specimens have been
deposited in the mycological collection of the Department of Plant Protection, Faculty
of Agriculture, Dicle University, Diyarbakır, Turkey (DUF-M).
For scanning electron microscopy (SEM), 8–10 mm squares of infected leaves were
mounted on SEM stubs with double-sided adhesive tape, coated with gold using a
Polaron SC 502 Sputter Coater, and examined with a Jeol JSM 6060 scanning electron
microscope at 5–10 kV in the Electron Microscopy Unit, Faculty of Science, Gazi
University (Turkey).
Taxonomy
Uromyces bornmuelleri Magnus, Verh. Ges. Deutsch. Naturf. 65: 151.1893. Figs 1–7
Spermogonia, aecidia and uredinia unknown. Telia amphigenous,
chestnut brown, in dense groups or scattered, 0.2–3 mm diam., causing leaf
deformation, at first covered by the epidermis, later becoming erumpent,
pulverulent. Teliospores yellow or yellowish-brown, ovoid, globoid,
sometimes oblong, 21–30 × 18–21.5 µm, rounded at the apex, rounded or
sometimes attenuate at the base; wall 2–4 µm thick, pedicels hyaline, short,
fragile.
Specimen examined — TURKEY, Adiyaman Province, Center, Kahta, Gerger, Sincik,
Çelikhan, Samsat, in wheat field ecosystem, on the leaves of Bongardia chrysogonum (L.)
Spach (Berberidaceae), 12.V.2009, C. Özaslan CÖ2029 (DUF-M).
Uromyces bornmuelleri has been reported from Azerbaijan on Leontice
chrysogonum [≡ Bongardia chrysogonum] (Tranzschel 1939), and from Iraq and
Cyprus on Bongardia chrysogonum (Georghiou 1957, Mathur 1972). Uromyces
bornmuelleri is reported for the first time from Turkey.
Physoderma maculare Wallr., Fl. Crypt. Germ. 2: 192. 1833.
Figs 8–13
Resting sporangia amphigenous, mostly hypophyllous and on leaf
petioles, concentrated on leaves along the nerves, scattered, covered by the
unbroken epidermis, ellipsoid, ovoid, sometimes punctiformis, 1–2 mm diam.,
chestnut brown. Resting spores chestnut brown, globoid, ovoid to ellipsoid,
content granular with more refractive globules, (23.5–)25.5–32 × 21.5–29 µm;
with two distinct walls, the inner wall thin and colorless, the outer wall chestnut
brown, 1–2 µm thick, smooth. Zoospores not seen.
Specimen examined — TURKEY, Siirt Province, Karaca Village, 38°07¢09²N
42°01¢59²E, in rice field ecosystem, on the leaves of Alisma plantago-aquatica L.
(Alismataceae), 8.VI.2012, C. Özaslan CÖ2035 (DUF-M).
The genus Physoderma and P. maculare are reported for the first time from
Turkey. This fungus has been reported from Canada (Conners 1967), Ireland
14 ... Özaslan & al.
Figs 8–13. Physoderma maculare. 8: Resting sporangia on leaf, in situ. 9: Resting spores. 10: Resting
sporangia on leaf (SEM). 11–13: Resting spores (SEM).
(Muskett & Malone 1984, as Cladochytrium alismatis), Poland (Czeczuga et
al. 2007), Siberia (De Toni 1888, as Uredo alismatis), Spain (Gonzalez Fragoso
1918, as U. alismatis), and U.S.A. (Farr et al. 1989).
Acknowledgements
The author thanks Prof. Dr. Z.M. Azbukina (Vladivostok, Russia) and Dr. Yuri
Tykhonenko (Kiev, Ukraine) for critically reading the manuscript and serving as
presubmission reviewers. Especially we are grateful to Dr. Shaun Pennycook (Auckland,
Uromyces and Physoderma spp. new for Turkey ... 15
New Zealand) for detailed linguistic help and nomenclatural review of the manuscript.
We would like thank DÜBAP (Dicle University Research Projects Coordinator) for
financial support of this Project (DÜBAP 12ZF71). We also thank Prof. Dr. Mecit Vural
(Gazi University, Ankara) and Prof. Dr. A. Selçuk Ertekin (Dicle University, Diyarbakır)
for his help with the identification of the hosts.
Literature cited
Bahçecioğlu Z, Kabaktepe Ş. 2012. Checklist of rust fungi in Turkey. [Mycotaxon 119: 494.]
http://www.mycotaxon.com/resources/checklists/Bahcecioglu-v119-checklist.pdf
Clinton GP. 1902. Cladochytrium alismatis. Botanical Gazette 33: 49-61.
Conners IL. 1967. An annotated index of plant diseases in Canada and fungi recorded on plants
in Alaska, Canada and Greenland. Research Branch, Canada Department of Agriculture,
Publication 1251. 381 p.
Czeczuga B, Muszynska E, Godlewska A, Mazalska B. 2007. Aquatic fungi and straminipilous
organisms on decomposing fragments of wetland plants. Mycologia Balcanica 4: 31–44.
Davis PH (ed.). 1965–85. Flora of Turkey and East Aegean Islands. Vols 1–9. Edinburgh University
Press, Edinburgh.
De Toni GB. 1888. Sylloge ustilaginearum et uredinearum. Sylloge Fungorum 7(2): 449–882.
Farr DF, Bills GF, Chamuris GP, Rossman AY. 1989. Fungi on plants and plant products in the
United States. APS Press, St Paul MN. 1252 p.
Georghiou GP, Papadopoulos C. 1957. A second list of Cyprus fungi. Government of Cyprus,
Department of Agriculture.
Gonzalez Fragoso R. 1918. La roya de los vegetales. Enumeracion y distribucion geografica de los
Uredales. Conocidos hasta hoy en la Peninsula Iberica e Islas Baleares. Trabajos del Museo
Nacional de Ciencias Naturales, Serie Botánica 15: 1–267.
Index Fungorum. 2014. http://www.indexfungorum.org/names/names.asp. Accessed 26 May 2014.
Kuprevich VF, Ulijanishchev V. 1975. Key to the rust fungi in SSSR. Minsk, Belarus: Nauka i
Tekhnika.
Mathur RS. 1972. Checklist of Iraqi Uredinales. Iraq Natural History Museum, Publication 29:
1–32.
Muskett A, Malone J. 1984. Catalogue of Irish fungi – V. Mastigomycotina and Zygomycotina.
Proceedings of the Royal Irish Academy, B. 84: 83–102.
Olson LW, Edén UM, Lange L. 1980. The endobiotic thallus of Physoderma maydis, the causal agent
of Physoderma disease of maize. Protoplasma 103: 1–16.
Saccardo PA, Sydow P. 1902. Supplementum universale, pars V. Sylloge Fungorum 16. 1291 p.
Sparrow FK. 1964. Observations on chytridiaceous parasites of phanerogams. XIII. Physoderma
maculare Wallroth. Archiv für Mikrobiologie 48: 136–149.
Sparrow FK, Johns RM. 1965. Observation on chytridiaceous parasites of phanerogams XVI.
Notes on Physoderma from Scirpeae. Archiv für Mikrobiologie 51: 351–364.
Tranzschel VG. 1939. Compendium of rusts of the USSR. Botanicheskiĭ Institut Akademii Nauk
SSSR. Moscow. 426 p.
Unger F. 1832 [“1833”]. Die Exantheme der Pflanzen. Wien. 422 p.
van der Merwe M, Ericson L, Walker J, Thrall PH, Burdon JJ. 2007. Evolutionary relationships
among species of Puccinia and Uromyces (Pucciniaceae, Uredinales) inferred from protein
coding gene phylogenies. Mycological Research 111: 163–175.
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