Studies in Fungi 4(1): 30–35 (2019) www.studiesinfungi.org
ISSN 2465-4973
Article
Doi 10.5943/sif/4/1/5
First record of Capnodium berberidis from India
Gautam AK1* and Avasthi S2
1
2
School of Agriculture, Faculty of Science, Abhilashi University, Mandi-175028, India
Department of Botany, Abhilashi Post Graduate Institute of Sciences, Mandi- 175008, India
Gautam AK, Avasthi S 2019 – First record of Capnodium berberidis from India. Studies in Fungi
4(1), 30–35, Doi 10.5943/sif/4/1/5
Abstract
Capnodium berberidis, a sooty mould fungus, previously reported only from Pakistan in
1978, was recently observed on Berberis lycium in Jot Pass, district Chamba of Himachal Pradesh,
India. Berberis lycium is a well-known small to medium sized spiny, woody, deciduous or
evergreen medicinal and ornamental plant distributed throughout temperate and subtropical regions
of the world. Disease symptoms appeared as thin, black to dark brown, easily removed superficial
layer of hyphae on various parts of host plant including leaves, stem, twigs and fruits. The detailed
studies on its morphology and taxonomy revealed it a sooty mould fungus Capnodium berberidis.
As per literature consulted, this is the first report of Capnodium berberidis from India and probably
second from world.
Key words – Berberis lycium – black mildew – Dothideomycetes – Himachal Pradesh – new
record – sooty moulds
Introduction
Capnodium introduced by Montagne (1849) is the type genus of Capnodiaceae (Friend
1965). Capnodiaceae is the most common family of sooty moulds belongs to order Capnodiales
and class Dothideomycetes (Batista & Ciferri 1963, Hughes 1976, Crous et al. 2009, Schoch et al.
2009, Chomnunti et al. 2011, 2014, Hyde et al. 2013, Wijayawardene et al. 2014, Liu et al. 2015).
The appearance of mycelium superficially as a network of septate, dark brown hyphae on the
surfaces of host with production of bitunicate asci are the main characters of this family. The
production of short or long narrow necked elongated pycnidia having conspicuous oval swelling
near the base, middle or apex and produce hyaline conidia inside the swollen part is found in
anamorphic part (Chomnunti et al. 2011, 2014). The asexual morph and sexual morph can be found
in the same or different hosts, however, for some the teleomorph is unknown (Chomnunti et al.
2011, 2014, Hyde et al. 2013). It is believed to be the largest family containing sooty mould species
which causes chlorosis and plant stunting disease.
Berberis lycium is a well-known medicinal and ornamental plant distributed throughout
temperate and subtropical regions of the world. It is a small to medium sized spiny, woody,
deciduous or evergreen shrubs or small trees with characteristic yellow wood and yellow or orange
flowers (Ahmed & Alamgeer Sharif 2009, Sood et al. 2012). The chemical constituents include
isoquinolone alkaloids, especially berberine and are used in medicine to cure the liver, neck and
stomach cancer, blood purification and mouth scent (Khan et al. 2016, Chander et al. 2017). A
sooty mould infection was observed on all plant parts of Berberis lycium from Jot pass area of
Submitted 20 December 2018, Accepted 28 February 2019, Published 19 March 2019
Corresponding Author: Ajay K. Gautam– e-mail – a2gautam2006@gmail.com
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district Chamba, Himachal Pradesh. Disease is easily identifiable by the presence of a black,
velvety growth covering various plants parts. The fungus produces mycelium which is superficial
and dark grows on the leaf, stem, flowers and sometime on fruits also. Critical morphological
examination of the diseased samples revealed it to be a species of Capnodium. Therefore, the aim
of this study was to investigate detailed morphological and taxonomic description of the
Capnodium species and compare it with already available literature on this fungus.
Materials & Methods
Infected plant parts of Berberis lycium were collected during winter (October 2016) in paper
bags from Jot pass area of district Chamba, Himachal Pradesh, India. These infected plant parts
along with a host twigs and reproductive parts were dried between sheets of blotting paper and
preserved for further studies. Host plants were identified and confirmed by matching the collections
with herbarium and by consulting botanists.
In the field, observations were made on few aspects, whether the disease occurs on old or
young leaves or on old or young trees. Infected plant parts were observed in the field, field notes
were made regarding their pathogenicity, nature of colonies, nature of infection, locality and
altitude. In the field, infected plants were collected in paper bags and taken to the laboratory for
identification and taxonomical studies. For herbarium, the infected plant parts were pressed neatly
and dried in between blotting papers. After ensuring their dryness, they were kept in the manifold
blotting or butter paper. Identified samples were deposited in the Abhilashi University Mycological
Herbarium (AUMH), Abhilashi University Mandi, Himachal Pradesh, India.
The diseases symptoms were first studied with the help of magnifying lens and then examined
under a stereomicroscope. Surface scraping of the aerial parts bearing the sooty mould fungus was
taken and mounted in lactophenol cotton blue mixture for microscopic examination. The
microscopic observations were made under oil immersion by standard light microscopy to note
down characters of mycelium, pycnidia and conidia. For microscopic characters and photographs a
research microscope connected with Sony DSC camera was used. All measurements were taken
with the help of ToupView software. All measurements are given in the form of min–max (mean
and number of measurements). The disease and pathogen was identified and compared with already
available literature (Mukherji & Juneja 1974, Bilgrami et al. 1991, Jamaluddin et al. 2004,
Chomnunti et al. 2011, 2014).
Results
The sample collection site Jot Pass, is well known hill station located in district Chamba of
Himachal Pradesh, India. It is located at 2300m height and have heavy snow fall during winter. The
species of Cedrus, Rhododendron and Quercus are chiefly found in the area along with small to
medium sized woody shrubs including Berberis. The climatic conditions of the areas are quite
favourable for growth of foliicolous fungi including sooty moulds (Fig. 1).
Disease symptoms appeared as thin, black to dark brown, easily removed superficial layer of
hyphae. The disease symptoms usually appeared in the winter season and infect all plant parts
including leaves, stem, twigs and fruits.
Taxonomy
Capnodium berberidis S. Ahmad, Monogr. Biol. Soc. Pakistan 8: 33 (1979)
Fig. 2
Fungus growing on the surface of leaves, branches, twigs and stems of Berberis lycium as thin
dark brown, easily removable layer on the host surface and composed of cylindrical hyphae.
Thallus of superficial mycelium 3–5 μm wide (x̅ =4 μm, n=15), septate, constricted at the septum,
branched, brown to dark brown. Asexual morph: Pycnidia 155–185 μm long (x̅ =165 μm, n=10),
superficial, scattered or gregarious, blackish brown, cylindrical, swollen at the central part, 15–22
μm diam. (x̅ =30 μm, n=10), lacking a basal bulbous part. Wall of pycnidia comprising mostly
cylindrical cells, the swollen part producing conidia inside. Ostiole of pycnidia 15–18 μm diam. (x̅
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=15 μm, n=10). Conidia 4–7 × 1–3 μm (x̅ =5 × 2 μm, n=15), cylindrical to oblong, brown, ends
round, hyaline, smooth-walled. Sexual morph: Undetermined.
Material examined – India, Himachal Pradesh, Chamba, Jot Pass, on leaves of Berberis
lycium Royle (Berberidaceae), 2880 meters, 8 October 2016, A. K. Gautam, (AUMH-1034).
Known distribution – Pakistan, India.
Fig. 1 – Collection site of Capnodium berberidis on Berberis lycium (Jot Pass, Chamba, Himachal
Pradesh).
Discussion
Capnodium is a widely distributed genus having a wide host range. There are 140 epithets
recorded on a large variety of plant hosts (www.indexfungorum.org; accessed 1 November 2018).
Number of species of Capnodium has been reported across India. Previously it was observed as
Capnodium citri on Citrus sp., C. eugeniarum on Ficus retusa, Agave vera-crucis (Sharma &
Mishra 2018), C. anonae on Ficus racemosa, C. ramosum on Mangifera indica, Capnodium sp. on
Nyctanthes arbor-tristis (Todawat 2017). A detailed comparison of Camptomeris spp. reported
worldwide along with host range is provided in this study (Table 1).
The sooty mould genus Capnodium is most commonly found in gardens and landscapes and
generally recognized with their superficial black mycelia with septate, cylindrical, dark-brown
hyphae, asexual morph as elongated pycnidia with short or long narrow neck. Conidia produced are
released through a short opening called ostiole (Laemmlen 2011, Chomnunti et al. 2011). Based on
these characters the present collection is identified as species of Capnodium. The critical analysis
of morphological characters of the diseased samples and their microscopic examination revealed
pathogen as fungus Capnodium berberidis.
Capnodium inhabits a wide array of hosts, no report is still available from any place of India.
The occurrence of Capnodium berberidis on Berberis lycium has only been reported from Pakistan
(Ahmad 1978) but not detected afterward across the globe. Therefore, we present here a new record
and new addition to mycoflora of India and probably second from world.
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Fig. 2 – Capnodium berberidis. A Substrate. B Superficial vegetative mycelium (septate hyphae)
C–E Pycnidia (Ostiole surrounded by hyaline hyphae). F conidia. Scale Bars: B=10 µm, C–E=100
µm, f=20 µm.
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Table 1 Comparison of Capnodium spp.
Species
Host
Capnodium berberidis S.
Ahmad
Capnodium citri Berk. &
Desm
Berberis
lycium
Citrus sp.,
Olea sp.
Capnodium coartatum
Chomnunti & KD Hyde
Capnodium tiliae (Fuckel)
Sacc.
Capnodium coffeicola
Hongsanan & K.D. Hyde
Psidium
guajava
Tilia
parviflora
Coffea sp
Mycelium
(μm wide)
3–5
Pycnidia
(μm long)
155–185
4.2–6
345–391
3–5
332–401
4–6
81–136
3–5
165–178
Conidia
μm
4–7 × 1–3
Reference
Ahmad 1978,
Present study
6.5×5
Rao 1970,
Chomnunti et al.
2011
4.2–
Chomnunti et al.
4.6×1.9–2.4 2011
13–17×5–7 Chomnunti et al.
2011
14–16
Hongsanan et al.
2015
Acknowledgements
The authors gratefully thank their respective organizations for providing laboratory facilities
to carry out this research work successfully. We also express our thanks for encouragement and
every possible support provided by everyone during this scientific study.
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