ISSN (print) 0093-4666
© 2012. Mycotaxon, Ltd.
ISSN (online) 2154-8889
MYCOTAXON
Volume 119, pp. 369–372
http://dx.doi.org/10.5248/119.369
January–March 2012
Second record of Ramularia hypericicola —
collected in Turkey on a new host
Faruk Selçuk1, Elşad Hüseyin1* & Ali S. Bulbul2
Department of Biology, Arts and Sciences Faculty, Ahi Evran University,
Kırşehir, 40100, Turkey
2
Department of Biology, Sciences Faculty, Gazi University,
Teknik okullar, Ankara, 06500, Turkey
*Correspondence to: elsadhuseyin@hotmail.com
1
Abstract – Ramularia hypericicola, a rare anamorphic Mycosphaerella species, is reported
and illustrated from Turkey on Hypericum calycinum. The Turkish collection represents the
second report of this fungus, known hitherto only from the type locality.
Key words — symptoms, Clusiaceae, morphology
Introduction
Most species of Ramularia are phytopathogenic, causing leaf spots,
sometimes chlorosis, or without visible symptoms, occasionally saprobic
or hyperparasitic (Braun 1998). Bremer & Petrak (1947) published the first
records of Ramularia species from Turkey, R. anatolica Bremer & Petr. and
R. helminthiae Bremer & Petr. Subsequent papers about Turkish microfungi,
including Ramularia species, were published by Bremer et al. (1948, 1952a,
1952b) and Petrak (1953, 1957). Additional Turkish records, of Ramularia
cynarae Sacc., R. onobrychidis Allesch., and R. sambucina Sacc., were published
by Karel (1958). The relatively small number (nearly 30 species) of Ramularia
species recorded in subsequent papers (Göbelez 1964) reflects a rather poor
degree of research on microfungi on plants in Turkey. However, during the
past two decades research on micromycetes (including hyphomycetes) in the
country has intensified (Altan & Tamer 1996, Hüseyinov & Selçuk 1999, Braun
et al. 2000, Hüseyin & Selçuk 2001, Hüseyinov et al. 2002, Hüseyin et al. 2003,
2005, Melnik et al. 2004, Erdoğdu & Hüseyin 2007, Selçuk et al. 2009, 2010).
We describe here one recent collection from Turkey of a Ramularia species
previously recorded only from Russia.
370 ... Selçuk, Hüseyin & Bulbul
Figure 1. Ramularia hypericicola on Hypericum calycinum; a, spots on infected host leaves;
b, punctiform grayish white caespituli; c, small fasicle of conidiophores; d, conidia.
Material & methods
The plant material was gathered from Gemlik (Bursa Province). The host plant was
identified by reference to Davis (1967). Sections of the fungus were hand cut using a
razor blade and microscopically examined using a Leica DM LB research microscope.
The fungus was identified following Braun (1998). Author abbreviations follow Index
Fungorum (2011). All examined specimens are deposited in the herbarium of Ahi
Evran University, Arts and Sciences Faculty, Department of Biology, Kırsehir Province,
Turkey.
Results
After careful comparison with Ramularia spp. described on hosts of
Hypericaceae based on Braun (1998) the recently collected material on
Hypericum calycinum from Turkey proved to be clearly identical with
R. hypericicola. The following description and illustration are based on the
Turkish material.
Ramularia hypericicola U.Braun, Monogr. Cercosporella, Ramularia 2: 170, 1998.
Fig. 1
On both surfaces of leaves causing subcircular to irregular, occasionally
angular and vein-limited, scattered sometimes confluent spots, covers more
than 50% of the surface of some leaflet, firstly 0.5–1 mm across, then up to 10
mm diam. Spots on upper surface dirty brownish, dull, lover surface clearly
A new host for Ramularia hypericicola (Turkey) ... 371
brown, without distinct margin. Caespituli hypophyllous, punctiform, grayish
white. Mycelium internal, colourless, hyphae septate, branched, formed
stromatic, colourless to yellowish-ochraceous, subglobose or slightly lengthened
hyphal aggregations in leaflet tissues, 15–70(–75) µm diam. Conidiophores in
small to moderately large fascicles, loose to usually dense, emerging through
stomata or erumpent through the cuticle, erect, simple, straight, subcylindrical
to geniculate-sinuous, continuous to sparingly septate, hyaline or subhyaline,
smooth, 6–65(–70) × 2.5–5 µm. Conidiogenous cells terminal, colourless,
enteroblastic. Conidiogenous scars thickened, darkened. Conidia colourless,
catenate, chains occasionally branched, produced enteroblastically, single
conidia smooth to faintly rough, ellipsoid-ovoid, subcylindrical, fusiform,
10–30(–35) × 4–5(–6) µm, unicellular or with a single median septa, not
narrower at the septa, ends rounded or somewhat attenuated.
Specimen examined – TURKEY, Bursa Province, Gemlik district, Haydariye
village, near Şelale, alt. 700 m, on living leaves of Hypericum calycinum L. (Clusiaceae),
in hornbeam–beech (Carpineto-Fagetum) forests, 18 Nov 2010, coll. E. Hüseyin G-06.
Discussion
In 1956, the Russian mycologist M.K. Khokhryakov collected a Hypericum
sp. with Ramularia-type sporulation in the Russian Far East, which he deposited
in LEP as Ramularia sp. Braun (1998: 170) described R. hypericicola as a new
species based on this material. Ramularia hypericicola is undoubtedly a rare
anamorph of Mycosphaerella. The present record from Turkey represents the
second collection of this species and the first on a known host, Hypericum
calycinum.
Acknowledgments
The authors would like to extend a sincere gratitude to Uwe Braun (MartinLuther-Universität, Halle/Saale, Germany) for linguistic help and confirmation of our
identification. We also thank Dr. Olga Vinnere Pettersson (Uppsala, Sweden) and Dr.
Tatiana Andrianova (Kiev, Ukraine) for critically reading the manuscript and serving
as presubmission reviewers. Especially we grateful to Dr. Shaun Pennycook (Auckland,
New Zealand) for detailed linguistic help and nomenclatural review of the manuscript.
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