! " ## $ $ # $ $ ! & "% ' % ( " ) # $ ## & # $% !** + % ,$ '()*+,) .., $ 2 A new species of . $% . % " , $ " $% , 0" 1 % $$ " are reported on . has been described on . $% $ and some new fungal records " , " " " / " , % 0 $% " sp., and $% $ for the first time from Pakistan. -)*./0,)1.. $% $ (as . $% 3" 0 . $% ) belong to the family 0 $ , Neem is its common name. It is important due to its commercial and medicinal value. . $% $ is also an important plant for its potential of anti fungal, anti bacterial and insecticidal activity. Decline of trees due to fungi are increasing tremendously in Pakistan especially in Punjab and Sindh (Javed ., 2004; Khanzada ., 2011; Fateh , 2011; Rheman , 2011; Farooq , 2011). Sixteen fungi has been reported on . $% $ from Pakistan viz., " $ var, $ (L.) Wiggers on trunk of . $% $ ;4 $ $ (Berk. & Curt.) Mueller on bark of . $% $ (as 0 . $% ); (Fr.) Rab, on stem of 0 . $% ; 2" " " Sacc.,on branches of . $% $ (as 0 . $% ); 2" " (Berk. & Br.) Berl, on dead branches of . $% $ (0 . $% ); "$ " (Curtis) Karst. (as "$ " ), on . $% $ (Neem); " Berk., causes wood rot in . $% $ (as 0 . $% ); / Nees Mont., causes white wood rot of . $% $ " (Nees. ex. Fr.) Wiltshire, causes leaf spot of . $% $ ; $ " Syd., causes leaf spot of . $% $ $ Ell. & Ev., causes leaf spot on . $% $ 5 " spp., cause powdery mildew in . $% $ ; . $% (Ell. & Ev.) Ahmad; causes die back of . $% $ ; 1 % (Pat.) Griffon & Maubl (as % Pat., on dead branches of . $% $ ; Sacc. & Roum., on leaf rachis of . $% $ ; % Ahmad on leaf rachis of . $% $ ; Ahmad, 1956, 1962, 1969, 1978; Ahmad & Arshad, 1972; Ahmad ., 1997; Ghaffar & Kafi, 1968; Ghaffar ., 1971; Ghaffar & Abbas,1972; Ghafoor & Khan, 1976; Khan, 1952, 1969, 1989; Khan & Kamal 1963, 1968 Kamal & Mughal, 1968; Malik & Khan, 1944. It is very surprising that only 16 fungi have been reported from Pakistan on . $% $ a widely cultivated and wildly grown tree. Why it is so? Whether this plant is neglected for fungal screening? or it is its antifungal, antibacterial and anti insecticidal activity that play a role to protect this plant from pathogens. For this reason a detailed survey of the plant for fungi was under taken in this study. +)2*1+3( +-/ 2)4./( Samples of . $% $ were collected from the different areas of District Faisalabad and Gojra. The different areas were G.C. University. Faisalabad, Agriculture University Faisalabad, Gutwala forest (park) Faisalabad, Tandlianwala and Gojra City. Methods and materials are the same as described Abbas ., (2010). Identification up to species level were carried out after consulting (Morris, 1963; Ellis, 1971, 1976; Carmichael ., 1980; Sutton, 1980; Ahmad, 1978; Ahmad ., 1997; Abbas ., 2004; Kirk, 2012). 2(03)( +-/ 1(,0((1.The fungus on . $% $ specimen G.C.U.F. Mycol. H. # 40 was identified as a . % " Died. ! " Died., $ . 14: 215 (1916); Sutton, The Coelomycetes:58 61 (1980) 2(,*15)1.- .6 )42 60-70( 0-/2* ()0/8 Conidiomata eustromatic, ostiolate, dark brown to black, 152 190 µm. Conidiophores absent. Conidiogenous cells hyaline, enterogenous. Conidia uniseptate, hyaline, cylindrical, rounded at ends, constricted at septa, 3.8 11.4 × 1.26 1.50µm Fig. 1(A E). . % Died is a monotypic genus based on . % " Died. Fungus under study on . $% $ was compared with the description of , " and found that the fungus under study completely resembled with , " , Therefore, the fungus on , $ is identified as , " , Genus . % has not been previously reported from Pakistan (Ahmad ., 1997). It is an addition to fungal flora of Pakistan and . $% $ is also a new host of . % " from Pakistan, 52,192- 2:+91-2/ . % " on bark of . $% $ ; Pensara Road Gojra, Pakistan; 22 April, 07; G.C.U.F. Mycol. H. # 40: S. Qaiser Abbas & Nabila Iftikhar. SYED QAISER ABBAS 2- 1., - The fungus on G.C.U.M.H.No.42 is described as . $% sp. nov. # ! # $ #% (5 -.; 2(,*15)1.- .6 )42 60-70( Colony blackish grey appearance on natural sample. Conidiomata pycnidial, separate, globose, thick walled and dark brown, 150 µm in diameter, ostiole absent. Conidiogenous cells hyaline, (16.5 21 × 3.5µm). Conidia aseptate, cylindrical, hyaline 12.25 16.5 (mostly 14µm) × 3.5µm and covered with gelatinous sheath which form cap like structure on apical side of conidia Fig. 2(A D). +)1- /2(,*15)1.- Conidiomata pycnidiala, separatata, globosa, unilocularia 150µm dia., ostiola absentia, conidiophora non observa, cellulae conidiogenae hyalinae (16.5 21 × 3.5 µm). Conidia aseptata, hyalinae., cylindricae, mucilaginae enclosa, ad apicem capitata 12.25 16.5 (14µm) × 3.5 µm. Holotypus . $% in ramis emortius . $% $ Green Town Gojra Pakistan; 3 May, 07; S. Qaiser Abbas & Nabila Iftikhar G.C.U.F. Mycol. H. # 42. Höhn., and Sacc. & March are two genera, which closely resemble with the fungus found on . $% $ . Resemblance lies In that both genera, have pycnidial conidiomata, dark brown, separate, globose, thick walled, of textura angularis and ostiole central, circular and papillate. Conidiophores absent. Conidiogenous cells hologenous and non proliferating, discrete, lageniform, hyaline and smooth. However they differ from each other in some respect. has aseptate conidia and has uniseptate conidia. The fungus under study on , $ has aseptate, cylindrical, hyaline conidia with gelatinous sheath, therefore it belongs to the genus , . $% resembles with (Pirozynski & Shoemaker) Nag Raj, in having straight, cylindrical, ellipsoido fusiform conidia with apical appendages but -, . $% differs from -, (Pirozynski & Shoemaker) Nag Raj in size of conidiogenous cells and conidia. Conidiogenous cells of -, are smaller and less wider (12 15×1.5 2.5µm) than -, . $% (16.5 21×3.5µm), whereas conidia in -, are longer and wider (20 29.5×7 9µm) than -, . $% (12.25 16.5 (mostly 14×3.5µm). Similarly in -, var. conidiogenous cells are smaller and less wider (12 18×1.5 2.5µm) than -, . $% (16.5 21×3.5µm). Conidiomata of -, Whitney, Reid & Pirozynski, -, (Berk. & Br.) Whitney, Reid & Pirozynski and -, " " Whitening, Reid & Pirozynski are larger and wider (550 600µm in diam.) than -, . $% (150 190µm in diam.). Furthermore in -, $ Sutton & Marasas, conidiomata are slightly bigger and wider (200µm in diam.) than -. . $% whereas conidia in -, $ are oval to fusiform, straight 29 38 x 12 17µm., more longer and wider conidia than -, . $% (16.5 21×3.5µm). -, (Subram. & Ramakr.) Nag Raj, closely resembled -. . $% in having slightly bigger conidiomata (200µm) than this fungus (150 190µm). Similarly the conidiogenous cells of -, are more smaller and less wider (8 10×2.5 4µm) than -. . $% (16.5 21×3.5µm) and conidia are straight, cylindrical, much longer and wider (19 27×4.5 6µm) than -, . $% [12.25 16.5µm (mostly 14µm) × 3.5µm]. It is clear that -, is near to -, . $% , but conidia of -, . $% are smaller and less wider, therefore the under study fungus is described as a new species. Previously the genus has not been reported from Pakistan (Ahmad ., 1997). The genus is a new addition to the fungal flora of Pakistan and . $% is a new species described from Gojra, Pakistan. 52,192- 2:+91-2/ . $% from dead branch of . $% $ ; Green Town Gojra Pakistan; 3 May, 07; G.C.U.F. Mycol. H. # 42; S. Qaiser Abbas & Nabila Iftikhar. The fungus on . $% $ specimen G.C.U.FMH. # 34 is identified as $ " $% " (Preuss) Simmons. & % $ % # # (Preuss) Simmons, 0 $ , 59: 88 90 (1967). 2(,*15)1.- .6 )42 60-70( 0-/2* ()0/8 Colonies effuse and black. Mycelium, superficial, black brown. Conidiophores dark brown, branched, morphologically different from vegetative hyphae, 30 52.5×3.5 7µm. Conidiogenous cells olivaceous brown, cylindrical, 7×3.5µm. Conidia broadly ellipsoidal or inversely ovoid, brown 1 5 oblique and transverse septa, beak not present, thick walled, echinulated, 11.4 34.2×7.6 15.2µm Fig. 3(A G). Genus $ " and show close resemblance with each other but differ in conidial attachment to conidiophores. In conidia are attached from their broader side while in $ " conidia are attached from their narrow side. $ " $% " (Preuss) Simmons, , $ (Thüm.) Simmons and , (Cooke) Simmons are characterized in having 1 5 transverse septa and several longitudinal or oblique septa, whereas , Preuss has 1 3 transverse speta. , " Preuss., and , " Simmons, have 3 5 transverse septa. , $ " Mouch has 2 7 transverse and several longitudinal and oblique septa. The fungus present on , $ has 1 5 transverse septa and several longitudinal and oblique septa. These characters are common in 3 species of $ " viz., , $% " , , $ and , , However in , $% " (Preuss) Simmons conidiophores are 50 × 5 7µm, conidia (18 38 × 11 20µm) in chains of 2 10 with small or false beaks. Whereas , $ and , differ from , $% " in that conidia are without beaks and are not in chains (16 34 × 10 15µm). In , $ conidiophores are (60 × 4 5µm), whereas in , conidiophores are 100 × 4 7µm and conidia are 18 35 × 15 20 µm, that is smaller than , $% " . In the fungus under study, conidial lower limit range 11.4 34.2 × 7.6 15.2µm is lower than , 18 35 × 15 20µm and , $ 16 34 × 10 15µm and conidia are less wider than , and , $ . NEW FUNGAL RECORDS ON 6 Fig. 1(A E). 4- . % 7 FROM PAKISTAN " < : A, B, C & D. Conidia.1000X; E. conidiomata.400X. Fig. 2(A D). . $% A. Conidia with mucilaginous cap. 1000X; B. Conidia with gelatinous sheath.1000X; C. Conidia.1000X; D. Conidiomata.400X. Fig. 3(A G). $ " $% " ) A. Conidiophores with conidial attachment; B. Conidiophore with unseptate conidia (400X); C. Conidiophore (1000X). D. Attachment of conidia; E. Chain of 3 conidia; F. Chain of 2 conidia; G. Conidia with variable septa number (D, F, G, 1000X; E, 400X). The fungus under study completely resembled with $ " $% " except some minor differences in conidial dimension, therefore, it is identified as $ " $% " . Three $ " spp., viz., $ " Preuss, , " Preuss., , $ (Thüm.) Simmons, have been reported from Pakistan (Ahmad, 1969; Nisa ., 1971; Matsushima, 1993). , $% " has not been previously reported from Pakistan (Ahmad ,, 1997). , $% " an addition to fungal flora of Pakistan and . $% $ is a new host record of , $% " , 52,192- 2:+91-2/ $ " $% " Preuss, on branches of , $ ; Green town Gojra Pakistan; 29 April, 07; G.C.U.F. M.H. # 34; S.Q. Abbas and Nabila Iftikhar. SYED QAISER ABBAS 2- 1., The fungus on . $% $ G.C.U.FMH. # 37 is identified as " Ellis & Everh. $ ! 8$ , % # " '# Ellis & Everh., . 1893: 463 (1894). specimen " $, $ , 7, 2(,*15)1.- .6 )42 60-70( 0-/2* ()0/8 Mycelium septate, superficial, pale brown. Conidiophores septate, brown, scars present 230 250×5 9µm. Conidia cylindrical to oval, attached at apex as well as at lateral sides of conidiophores, 0 3 septate, 11.4 16 × 3.8 8µm Fig. 4(A E). The fungus under study on . $% , $ has 0 3 septate conidia. " " Ellis & Everh, , $ Berk.& Brown, , " $ Speg., , $ $ " Preuss, , (Cooke) de Vries, and , $ (Ellis & Barthol.) Ellis have 0 3 septate conidia. Inspite of similarity in having 0 3 septate conidia, howevere there are sufficient differences among them are found, which separate them from one another. " $ have shorter conidiophores (150×6 9µm) with terminal and intercalary swellings of 10 12µm. than fungus under study where conidiophores are 230 250×5 9µm; and without terminal and intercalary swelling. Similarly , $ also have wider conidiophores 260×6 8µm than fungus under study 230 250×5 9µm whereas , have long conidiophores 350×6 8µm than fungus under study which has conidiophores of 230 250×5 9µm and " $ $ " differs from fungus under study in having longer and less wider conidiophores 300×4 8µm with terminal and intercalary swellings of 9 11µm diameter. Furthermore " " $ also differs from under study fungus in having longer and less wider conidiophores 300×3 5µm than the under study fungus where conidiophores are 230 250×5 9µm. Furthermore conidiophores in , " are wavy, smooth, reddish brown, septate, 250 × 5 9µm; Conidia cylindrical, narrowing at the ends, lemon shaped, in simple or branched chains, smooth, light brown, 5 15×4 7µm. After comparison it is concluded that the under study fungus on . $% $ closely resembled with , " , hence it is identified as , " . The fungus on , $ identified as , " has not been previously reported from Pakistan (Ahmad , 1997) and it is a new report on , $ from Gojra Pakistan. chains. Chains consist of 3 4 conidia (Fig. 4). Colony on . $% $ has green appearance Fig. 5(A E). " $% " Cooke & Massee, , $ (Ellis & Barthol.) Ellis, , " $ Speg., , " Ellis & Everh., , $ $ " Preuss and , (Cooke) de Vries have 0 1 septate conidia and differ from under study fungus which has 0 3 septate conidia. However, " / " Berk. & Curt., , % " (Pers.) Link ex Gray, , $ $ Sawada, , $% E. A. Ellis & M. B. Ellis, , $ Sutton, , $ $ Thüm, and " " " Cooke have 0 1 septate conidia and resemble with under study fungus in this regard, however there are some differences which separate each from other " $ has longer and wider conidiophores (250×5 9µm) than under study fungus (152 268×3.8µm). Similarly , $ $ (100×4 6µm) and " $% (100×3 8µm) have shorter and slightly wider conidiophores than under study fungus (152 268×3.8µm). Further more , " " has much longer conidiophores (800×3 6µm) than under study fungus (152 268×3.8µm) and C, % " has conidia, smaller and wider (5 23×3 8µm) than under study fungus (10 19×3.8 5.7µm). Fungus under study completely resembles with " / " where conidiophores can easily be differentiated from vegetative hyphae, straight or slightly wavy, swollen nodes near the apex and upto 500 µm long and 3 5 µm wide with terminal and intercalary swellings (6 8 µm). Conidia arising from terminal swellings which later become smooth, intercalary, forming short simple and branched chains, (5 30×3 6µm). Fungus on . $% $ shows its complete resemblance with , / " , therefore it is identified as , / " . Previously " / " Berk. & Curt., has been also recorded on 25 different plants belonging to different families from Pakistan (Ahmad ., 1997). However it is first time recorded on . $% $ from Gojra Pakistan. 52,192- 2:+91-2/ " / " on leaves of . $% $ ; Quaid.e.Azam School, Gojra, Pakistan; 19 September, 07; G.C.U.F. Mycol. H. # 36 S. Qaiser Abbas & Nabila Iftikhar. The fungus on . $% $ specimen G.C.U.FMH. # 38 is identified as $ Henn. $ 52,192- 2:+91-2/ " " from leaves of . $% $ ; Green Town Gojra Pakistan; 10 September, 07; G.C.U.F. Mycol. H. # 37; S. Qaiser Abbas & Nabila Iftikhar. The fungus on . $% $ specimen G.C.U.FMH. # 36 is identified as " / " Berk. & Curt $ ! # ( ! # Berk. & Curt., (1868); Ellis. More Dematiaceous Hyphomycetes, CAB(IMI), Kew, Surrey, England, pp. 507, (1976). 2(,*15)1.- .6 )42 60-70( 0-/2* ()0/8 Mycelium superficial, light brown, septate. Conidiophore olivaceous brown, macronematous, 152 268×3.8µm, chlamydospores present, 7.6 µm. Conidia sub rounded, ellipsoidal to cylindrical, 0 1 septate, 10 19×3.8 5.7µm, solitary or in ! % )) Henn 4 9 , 41: 148 (1902). 2(,*15)1.- .6 )42 60-70( 0-/2* ()0/8 Mycelium septate, branched. Synnemata capitate, 816×38µm, apex broad, 49.4 68.4µm; hyaline, thick and steriated. Conidiophores 19 27×3.8µm. Conidia uniseptate, obovoid to cylindrical, apex and base obtuse 4.6 6.5×3.04 3.8µm. Fig. 6(A G). Under study fungus found on . $% $ resembles with genera " Sacc., and Hennings. Both genera have tall cylindrical stipe. Conidia uniseptate, oblong or cylindrical but the difference between the two genera is that in " conidia are dry and in conidia were produced in mucoid mass.The fungus isolated from . $% $ (Neem) closely resemble with genus . The fungus from , $ was identified as $ Henn., after consulting Morris, (1963); Ellis (1971, 1976). NEW FUNGAL RECORDS ON 6 4- 7 FROM PAKISTAN = Fig. 4(A C). " " ) A. uniseptate conidia with prominent scars; B. uniseptate conidia; C. biseptate conidia; (A, B, C, 1000X). D & E. Conidial attachment (D & E, 400X). Fungus on . $% $ specimen G.C.U.FMH. # 39 is identified as 0" (Körb.) D. Hawksworth 0" (Körb.) D. Hawksworth. ,7 , 132(3): 289 (1979) :2 $ $$" " (Körb.) Th. Fr, :0 $ % $ Anzi 8 $, , 8$ , , ;0 ), 9: 256 (1866) :0 $ % (Körb.) Körb., (1855) :0" (Körb.) D. Hawksw, , 7 , 132(3): 289 (1979< var. :0$ " " (Körb.) Jatta, (1900) : " (Körb.) Tuck , $%, ( % ): 272 (1872) :8 $% (Körb.) Trevis , (1860) :- $% % $ " " Körb $% , ; "<: 467 (1865); Ahmad, Ascomycetes of Pakistan, part 1. $ $ , monograph 137(1978). *- $% % $ " " , $ " Anzi & G. Winter (1885) =- $% % $ " " Körb $% , ( "): 467 (1865< var. " Fig. 5. " / " . A, B & C. aseptate & uniseptate conidia (A, B, C 1000X). D & F. Conidiophores with conidial attachment (D400X; E1000X). Previously $ % has been reported on dung from Faisalabad Pakistan (Mirza & Qureshi, 1970). , $ is a new fungal record from Pakistan and . $% $ is a new host for this fungus from Faisalabad, Pakistan. 52,192- 2:+91-2/ , $ from bark of . $% $ ; Tandlianwala district Faisalabad Pakistan; 15 September, 07; G.C.U.F. Mycol. H. # 38; S.Q. Abbas & Nabila Iftikhar. 2(,*15)1.- .6 )42 60-70( 0-/2* ()0/8 Ascocarp flask shaped, dark brown, superficial, ostiolate with colorless appendages on ostioler region, 425.5 485.5µm. Asci clavate, 28 35×10.5 14µm. Ascospores hyaline, many, oval to ellipsoidal, uniseptate, 7×3.5µm. Fig. 7(A E). This fungus 0" (Körb.) D. Hawksworth was reported by Ahmad (1978) and Ahmad ., (1997) as - $% % $ " , " from Changa Manga Pakistan on 8 , Hawksworth (1979) replaced it to 0" (Körb.) Hawksworth. Fungus under study completely resembled with 0" (Körb.) Hawksw (syn.-, " Köerb) in having ascocarp of flask shaped, dark brown, superficial and ostiolate, 425.5 485.5 µm; appendages hyaline at ostioler region. Asci clavate, 28 35×10.5 14µm. Ascospores hyaline, many, uniseptate, oval to ellipsoidal, 7×3.5µm . $% $ is a new host for 0" (#= .) D. Hawksw from Gojra Pakistan. 52,192- 2:+91-2/ 0" (Körb.) D. Hawksw as - $% % $ " " ) on bark of . $% $ ; Bilal park Gojra Pakistan; 20 April, 07; S.Q. Abbas & Nabila Iftikhar. G.C.U.F. Mycol. H. # 39. SYED QAISER ABBAS 2- 1., > Fig. 6(A G). $ : A synnemata (60X); B,C capitates head of synnemata 9400X); D,E,F,G uniseptate conidia (1000X). Fig. 7. 0" ) A. Ascocarp 400 X; B. Ascocarp with asci 400 X; C. Crushed ascocarp. 400X; D. Ascospores. (1000X). E. Asci.1000X. Fig. 8(A C). 1 % : A. Hyaline conidia. 1000X; B. Mature conidia.1000X; C. Conidia with conidiogenous cells.1000X. NEW FUNGAL RECORDS ON 6 Fungus found on . GCUF#42 is identified as 1 (Sacc) Keissl. 4- $% 7 $ ! $ $ # ! # (Sacc.) Keissl., 6 ,, Abt. 2 36: 314 (1918) : % Sacc., " , 8 $, , 35: 130 (1896) FROM PAKISTAN specimen % %, , , ., 2(,*15)1.- .6 )42 60-70( 0-/2* ()0/8 Brownish black patches on the bark of . $% $ . Conidiomata pycnidial, non ostiolate, dark brown, thick walled, 296 µm. Conidiophore absent. Conidiogenous cells hyaline, unseptate, thin walled, 9 9.5×3.8µm. immature conidia hyaline, aseptate, oval and thickwalled, at maturity conidia become brown, uni septate, thick walled with longitudinal striations, from apex to base 30 33.4×14 16µm Fig. 8(A C). Sutton (1980) was of the opinion that correct name of % was 1 % . +92 .6 (52,12( 1, 1, $ $ 1, $ 1, 1, 1, " 1, % . 1, 1, $ 1, % 0-70( 0-/2* ()0/8 1, 1, " 1, " % 1, $ 1, " 1, % 1, % 1, " " 1, ." " " .-1/1+3 92+(0*292-) ?9 25 28 × 13 15 22.5 26.6 × 13.6 17.2 27 30 × 14 17 24 26 × 12 15 28.6 33.4 × 15.6 17.6 32 36 × 16 18.5 19.6 23.4 × 11.7 13.3 18.7 22.7 × 12.1 13.9 14 17 × 11 12 30 32 × 15 16 30 33.4 × 14 16 µm 18.3 22.1 × 10.7 12.3 26.7 32.5 × 14.4 16.7 25.5 30.5 × 14.8 17.2 21.7 26.3 × 13.4 14.8 16 19 × 10 11 24 33 × 13 17 23.6 28.8 × 13 15.4 22.4 24.2 × 12.9 14.3 28 30 × 11 12 20 32 × 13.5 19.2 26 33 × 12 15 Fungus under study differs from the following spp., in having bigger conoidia viz., 1, (25 28×13 15 µm), 1, $ $ (22.5 26.6×13.6 17.2 µm), 1, $ (27 30 × 14 17 µm), 1, (24 26 × 12 15 µm), 1, % . (19.6 23.4 × 11.7 13.3 µm), 1, (18.7 22.7 × 12.1 13.9 µm), 1, $ (14 17 × 11 12 µm), 1, (18.3 22.1 × 10.7 12.3 µm), 1, " % [(25.5–30.5× 14.8– 17.2 µm, Alves ,, 2008). (21.7 26.3 × 13.4 14.8, Abdollahzadeh ., 2010)], 1, ricinii (16 19 × 10 11 µm); 1, % [ ( 23.6 28.8 × 13 15.4 µm; Alves ,, 2008), (22.4 24.2 × 12.9 14.3 µm, Abdollahzadeh ., 2010)]. Howevere 1, " (32 36 × 16 18.5 µm) has more longer and wider conidia than the fungus under study. 1, (28.6 33.4 × 15.6 17.6 µm ), 1, % (28 30 × 11 12 µm) and 1, " " (20 32 × 13.5 19.2 µm) differ from the fungus under study by having smaller conidia, since their upper limit of conidial length is near or less than the lower length range of fungus under study. 1 Howevere, Punithalingum (1980) dealt it as % in his monograph, Abbas ., (2004) when reassessing the 8 % " " , they pointed out that 8 % " " Berk & Curt., was an earliar name for 1 % . After 2004, work on 1 was carried out both morphological as well on DNA finger printing and sequence (Pavlic ., 2004; Burgess ., 2006; Damm ., 2007; Alves , 2008); Abdollahzadeh ., (2010) dealt 14 species of 1 . Abdollahzadeh ., (2010) was of the opinion that conidial dimension of % never exceed 30 µm in length and 16 µm in width, while the conidial length in 1 " " are up to 32µm and width is up to 19.2µm therefore they consider that both species are separate taxa. Total species of 1 spp., are tabulated with reference to conidial measurement (Abdollahzadeh , 2010). 262*2-,2 Saccardo (1913) Abdollahzadeh ., (2010) Burgess ., (2006) Saccardo (1913) Abdollahzadeh ., (2010) Pavlic ., 2004 Abdollahzadeh ., (2010) Abdollahzadeh ., (2010) Pavlic ., (2008) Saccardo (1913) *2(2-) ()0/8 Alves ., (2008) Damm ., (2007) Alves ., (2008) Abdollahzadeh ., (2010) Saccardo (1913) Burgess ., (2006) Alves ., (2008) Abdollahzadeh ., (2010) Saccardo (1913) Abbas ., (2004) Burgess ., (2006) Further more conidial width of 1, " " is more wider than the conidial width of fungus under study. Fungus under study (30 33.4 × 14 16µm) more closely resembles with 1, % (30 32 × 15 16 µm), therefore it is identified as 1 % 1 % is for the first time reported from Gojra, Pakistan. 52,192- 2:+91-2/ 1 % is on bark of . $% $ ; 305 J.B. Gojra, Pakistan; 10 April, 07; S. Qaiser Abbas & Nabila Iftikhar G.C.U.F. Mycol.H. # 42. Fungus found on . $ $ $ > G.C.U.F. Mycol. H. # 44is identified as 0 $% " (Bat. & Bezerra) Muthumary, Abbas & Sutton. + " % " # " (Bat. & Bezerra) Muthumary, Abbas & Sutton, , , 0 $ , 8 $., 87(1): 103 108, (1986). SYED QAISER ABBAS 2- 1., 2(,*15)1.- .6 )42 60-70( 0-/2* ()0/8 Conidiomata eustromatic, globose, dark brown with rough surface, 114µm. Conidiogenous cells hyaline, cylindrical, hologenous, 5 7 × 2µm. Conidia 3 euseptate, ellipsoidal, hyaline, simple appendages or beak on both sides, 20.9 26.6 × 3.8µm. The length of basal appendage 2 2.85µm, the length of terminal appendage 5.7 7.6µm. Fig. 9(A C). Genus 0 $% (Sacc.) Allesch. (1902) and 0 $% " Muthumary, Abbas & Sutton (1986) show close resemblance with under study fungus. and have already been reported from Pakistan. Genus 0 $% (Sacc.) Allesch. is characterized by acervular conidiomata and 4 euseptate conidia. While 0 $% " Muthumary ., (1986) has eustromatic conidiomata and 3 euseptate conidia. The under study fungus completely matches with 0 $% " in having similarity in conidiomata, conidiogenous cells, conidiophores and conidial measurements. Therefore the fungus under study was identified as 0 $% " , Previously 0 $% " was reported from Pakistan on stem of $ " and 0 % Muthumary ., (1986). . $% $ is an addition to host list of 0 $% " from Faisalabad Pakistan. Fig. 9(A C). 0 $% B. Conidium.1000X; cells.1000X. ) A. Pycnidiom.100X; with conidiogenous Fig. 10(A E). . A. Conidiogenous cell; B. uniseptate conidium; C. biseptate conidium. (A, B, C, 1000X), D. Conidial attachment; E. Conidia. (D & E, 1000X). > G.C.U.F. simple and branched chains of variable in shape with I several transverse and longitudinal or oblique septa. The size of conidia of $" (Berk. & Br.) Ellis., -, $ (Dearn. & Barthol.) Ellis., and -, $ 9 (Sacc.) Ellis also differ from the conidia of the fungus under study in having smaller conidia. Conidia are 10 30×8 25µm in -, $" ; 14 50×7 26µm in -, $ and 8 20×5 9µm in -, $ 9 , whereas conidia of under study fungus are 45.6 182.4×7.6 15.2µm. Furthermore -, $ and -, " " have solitary conidium, often forming fork like structure, clavate and smooth or slightly verrucose and obtuse at the ends but both species differ in conidial size. In -, $ conidia are 12 38×4 10µm and conidia in -, " " are 5 20×5 14µm. Therefore it is concluded that the species under study does not match with any Fungus found on . " C. Conidia $ Mycol. H. # 35 is identified as - $ $ sp. D2(,*15)1.- .6 )42 60-70( 0-/2* ()0/8 Colonies dark brownish black on natural substrate. Stroma large, thick and brown. Conidiophore micromenatou slightly different from vegetative hyphae, unbranched, straight or wavy, pale brown and verrucose, 21 23×6 7µm. Conidiogenous cells terminal and cylindrical, 9 10×7µm. Conidia thick walled, highly variable in size and shape, 1 several transverse and oblique septa, brown, 45.6 182.4×7.6 15.2µm. Fig. 10(A E). $" (Berk. & Br.) Ellis., -, $ (Dearn. & Barthol.) Ellis., and -, $ 9 (Sacc.) Ellis., differ from -, $ Corda, and -, " " (Corda) Hughes by forming conidia in 52,192- 2:+91-2/ 0 $% " on bark of . $% $ ; G.C.University Faisalabad Pakistan; 26 April, 07; S. Qaiser Abbas & Nabila Iftikhar G.C.U.F. Mycol. H. # 44. NEW FUNGAL RECORDS ON 6 4- 7 FROM PAKISTAN species of and looks a new species, will be published in some where else. Previously " " (Corda) Hughes., has been reported on dead branches of un known host; Ahmad (1977) and (Lind.) Hughes on dead branches of un known host; Ahmad (1977) and from Khanspur Streams, Dadar Streams from Pakistan (Iqbal & Bhutty, 1979, 1980; Iqbal & Shahbaz, 1990). . $% $ is a new host record of sp., from Faisalabad Pakistan. However the fungus found on G.C.U.F. Mycol. H. # 43. identified as 2 % $ " on Neem tree is first report in this connection from Faisalabad, Pakistan. 52,192- 2:+91-2/ 2 % $$ " on bark of . $% $ ; G.C. University Campus Alama Iqbal Road, Faisalabad, Pakistan; 27 April, 07; G.C.U.F. Mycol. H. # 43; S. Qaiser Abbass & Nabila Iftikhar. 52,192- 2:+91-2/ ,, on branch of . $% $ ; Samanabad Gojra Pakistan; 25 April, 07; G.C.U.F. Mycol.H. # 35; S.Q. Abbas & Nabila Iftikhar.. Fungus found on . $ $ specimen # G.C.U.F. Mycol. H. # 43. 2 % $ " (Harz) Langeron et Miloch 2 % $ " (Harz) Langeron et Miloch., (1930); Beneke, E. S. Medical mycology laboratory manual, pp. 66, (1962). D2(,*15)1.- .6 )42 60-70( 0-/2* ()0/8 Colonies white in color when grow on natural substratum. Mycelium superficial, thin, long and branched. Conidiophores hyaline and branched. Macro conidia found, 3 septate, clavate, 10.5 17.5×3.5 7µm. No microconidia are found. 0$ " Velen., and 2 % Sabourd are two closely related hyphomycetous genera, resemble each other in many aspects. Both have velvety colonies, grow rapidly on medium with tan to brown in color. Hyphae septate, large. Macroconidia hyaline, multicellullar, transversely septate, thick or thin walled. However they also differ from each other. In genus 0 $ " microconidia may appear on short hyphae. Macroconidia fusiform, often have an annular frill. Chlamydospores may present a fungal colony of buff to brown in color while in 2 % colony color changes from buff to white at maturity. Macroonidia smooth and clavate shaped with obtuse ends. They are found singly or in clusters, Fig. 11(A C). Characteristics of under study fungus completely matched with 2 % $ " (Harz) Langeron & Miloch. 2 % $$ " is generally human pathogen recorded from the world (Kazmi, 2004, Bundu & Pavihran 2002; Macit, 2005; Mohmoudabadi, 1997 and from Pakistan (Khan & Anwar, 1969, Dilnawaz & Naseer, 2001; Hussain ., 1994; Thebo ., 2006). Recently a project on Dermatophytes of District Faisalabad was carried out collaboration of Department of Botany, G.C. University Faisalabad and Department of Dermatology. HO Faisalabd where one hundred and seventy nine patients were studied for dermatophytic infection. In 11 patients 2 % $ " was isolated (7 13%). This is a high % of occurrence of it a there is a big question that how this pathogen perpetuate in this area? Is this soil borne or borne in other substrate? The isolation from Neem tree is further alaraming to the mycologist working on plant pathogenic fungi. Generally it is supposed that they are non pathogenic to animals and human being. Fig. 11(A C). 2 % $ " ) A. triseptate conidium.; B. Conidia attached with conidiophore. C. Mycelium (A,B,C ;1000X.) 262*2-,2( Abbas, S.Q., I. Ali, M. Niaz, R. Ayesha and T. Iftikhar. 2010. New fungal records on 0 " from Faisalabad Pakistan I. , 3, ., 42: 583 592, Abbas, S.Q., B.C. Sutton, A. Ghaffar and A. Abbas. 2004. Reassesment of 8 % " " , , 3, ., 36(1): 209 218. Abdollahzadeh, J., A. Javadi, E. Mohammadi Goltapeh, R. Zare and A.J.L. Phillips. 2010. Phylogeny and Morphology of four new species of 1 from Iran , 25: 1 10 Ahmad, S. 1956. Fungi of Pakistan. , 8 $, . Lahore, Monogr. 1: 1 126. Ahmad, S. 1962. Further contributions to the Fungi of Pakistan. II. , 8: 123 150. Ahmad, S. 1968. Contributions to the Fungi of Pakistan. VII. , 14: 1 11. Ahmad, S. 1969. Fungi of Pakistan. , 8 $, , Lahore, Monogr. 5, Suppl. 1, pp. 110. Ahmad, J., 1967. A contribution to the microflora of Lahore soil, , 13: 1 3. Ahmad, S. and M. Arshad. 1972. Contribution to the fungi of West Pakistan. 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Botryshaeriaceae as potential pathogens of " " in South Africa with descrtption of $ and 1 " sp.nov. 0 $ , 99: 664 680. Dilnawaz, M. and R.D. Naseer. 2001. Comparison of various diagnostic Modalities in . 3, , $, ., 11(1): 625 7. Ellis, M.B. 1971. Dematiaceous Hyphomycetes, CAB(IMI), Kew, Surrey, England, pp. 608. Ellis, M.B. 1976. More Dematiaceous Hyphomycetes, CAB (IMI), Kew, Surrey England, pp. 507. Farooq, S., S.N. Khan, S. Naz, G. Mohy ud din and S.H. Khan. 2011 Distribution of Charcoal rots of 8 " $" L. (Sesame) in Punjab, Pakistan. 2011 Challengs and options $ , for plant health management ? % 7 % % $ 8 $ . Nov. 28 29, 2011.Faisalabad Agriculture University Faisalabad Pakistan, Page. 49. Fateh, S.F., M.R. Kazmi, I. Ahmad and T. Mukhtar. 2011 Common fungi found in decline affected Mango and Guava orchids in Punjab Challengs and options for plant health management ?% 7 , % % $ 8 $ . Nov. 28 29, 2011.Faisalabad Agriculture University Faisalabad Pakistan. Page. 30. 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Tinea unguium in the North West of Iran. , 0 $ , 24: 113 117. Khan, A.H. 1952. Wood rotting fungi of Pakistan and their control. 3, 8$ ., 4(2): 65 85. Khan, A.H. 1969. The control of wood inhabiting fungi. $, , " $ , Karachi. pp. 176. Khan, A.H. 1989. Pathology of trees. University of Agriculture Faisalbad. Vol. II: pp. 379 382. Khan, M. 2001. Leaf Spot of . $% $ A. Juss., caused by $ " H. Syd. Recorded in Bangladesh, VOL 25; PART 1, pages 105 108. Khan, K.A. and A.A. Anwar. 1969. The etiology of $" in Karachi. , 1, ., 81: 858 860. Khan, K.A. and A.A. Anwarullah. 1969. The etiology of $ " in Karachi. , 3 " , ., 81: 858 860. Khan, S.A. and M. Kamal. 1963. $ of the Sind region including 35 new records. , 3, 8$ , , ., 6: 118 119. Khan, S.A. and M. Kamal. 1968. The fungi of South West Pakistan. Part I. , 3 " , 8$ , @ , ,, 11: 61 80. Khanzada, M.A., A.M. Lodhi, A.Q. Rajput and S. Shazad. 2011. Evolution of different varieties to mango decline pathogen 1 % 2011. Challengs and options for plant health management ? % 7 , % % $ 8 $ . Nov. 28 29, 2011.Faisalabad Agriculture University Faisalabad Pakistan, Page 25. Kirk, P. 2012. CABI Bioscience data base. Index of fungorum. Macit, I. 2005. Onychomycosis in Adana from the Department of Medical Microbiology Faculty of medicine, University of Cukurova, Adana, Turkey, 44(10): 851 854. Mahmoudabadi, A.Z. 1997. A survey of 382 suspected patients with $ (Ahwaz). 8$ , 0 , 3., 22: 45 52. Malik, S.A. and M.A. Khan.1944. Parasitic Fungi of the North West Frontier Province. ,3 " , , 8$ , 13: 522 527. Matsushima, T. 1993. List of microfungi from Pakistan soil. In: crypt. Fl. Pak. Vol. II (Eds.): T. Nakaike & S. Malik, pp. 43 63. Nat. Sci. Mus. Tokyo. Mirza, J.H. and M.S.A. Qureshi. 1970. $ % sp. nov. , , 0 $ , 8 $, 54(1): 148. Morris, F.E. 1963. The Synnematous genera of the fungi immperfecti. Macomb Western Illionis University. Series in the Biological Science No :1 93 Muller, E. and J.A. Arx. 1962. Die Gattungen der didymosporen Pyrenomycetes Bietr # , 8$%9 , 11: 1 922. Muthumary, J., S.Q. Abbas and B.C. Sutton. 1986. A re assessment of 0 $% ., ,0 $ , 8 $, 87(1): 103 108. Nisa, A.U., S.I. Ahmad and S.A. Hussain. 1971. Studies on soil fungi, Part II. Fungi from P.C.S.I.R. Nursery soil. , 3, 8$, @ , ., 14: 232 23. Palvic, D., B. Slippers, T.A. Coutinhu, M. Gryzenhout and M.J.Wingfield. 2004. 1 sp.nov., a new % % from native 8 . " $ " South Africa. -" 0 $ , 50: 313 322. Palvic, D., M.J. Wingfield, P. Baber, B. Slippers, G.E.S. Hardy and T.I. Burgess. 2008. Seven new species of Botryspaertaceae from baobob and other natives trees in Western Australia. 0 $ , 100: 851 866. Punithalingum, E. 1980. Plant diseases attributed to % Pat. Camar, Vaduz. Rheman, A., R. Anjum, I.A. Khan, A.S. Khan and I. Ahmad 2011. Morphological characterization of $ sp. nov, isolate cause of Mango sudden death syndrome from Pakistan Challengs and options for plant health management ? % 7 $ , % % $ 8 $ . Nov. 28 29, 2011. Faisalabad Agriculture University Faisalabad Pakistan Page 41. Saccardo, P. 1913. Sylloge Fungorum Omnium hucusque Cognitorum, 22 1012. Sutton, B.C. 1980. The Coelomycetes Fungi Imperfecti with pycnidia Acervuli and Stromata. CAB (IMI), Kew, pp. 24 641. Thebo, N., K.H. Abro, A.Q. Soomro, J. Anwer and M. Shail. 2006. Isolation and identification of dermatophytes from Sindh, Pakistan. , 3, ., 38(2): 493 495. (Received for publication 27 July 2009)