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Genotypic analysis of the foliose lichen Parmotrema tinctorum using microsatellite markers: association of mycobiont and photobiont, and their reproductive modes

Published online by Cambridge University Press:  29 March 2012

Mohammad Reza MANSOURNIA ,
Bingyun WU ,
Norihisa MATSUSHITA  and
Taizo HOGETSU
Mohammad Reza MANSOURNIA
Affiliation:
Laboratory of Forest Botany, Department of Forest Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo. 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. Email: mrmansournia@yahoo.com
Bingyun WU
Affiliation:
Laboratory of Forest Botany, Department of Forest Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo. 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. Email: mrmansournia@yahoo.com
Norihisa MATSUSHITA
Affiliation:
Laboratory of Forest Botany, Department of Forest Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo. 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. Email: mrmansournia@yahoo.com
Taizo HOGETSU
Affiliation:
Laboratory of Forest Botany, Department of Forest Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo. 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. Email: mrmansournia@yahoo.com
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Abstract

To investigate the association between, and the reproductive modes of, mycobiont and photobiont in the lichen Parmotrema tinctorum, microsatellite markers (SSR markers) were developed for both bionts and genotypic analysis was performed for thalli in a Pinus thunbergii forest. In a within-thallus analysis, tissue pieces were sampled from many portions within each of five thalli. Within-thallus variation for both mycobiont and photobiont differed among the thalli investigated, suggesting that a single thallus can be derived from either a single mycobiont origin with or without occasional changes in the photobiont, or fusion of several independent thalli. In the within-tree analysis, thalli with the same genotypes were found on individual trees, suggesting the presence of short-distance isidium dispersal. In the within-stand analysis, genotypically similar photobionts tended to be associated with the same mycobiont genotype, suggesting the participation of local dispersal via isidia. The overall index of association (IA) for the mycobiont in the stand probably indicates the dominance of sexual reproduction, in contrast to that of the photobiont, where dominance of asexual reproduction is suggested. IA's of mycobiont and photobiont were also calculated for thalli collected from larger areas, where there is a signature for sexual reproduction for photobiont.

Keywords

clonalitydispersalIA analysissexual and asexual reproductionTrebouxia corticolawithin-thallus analysis
Type
Research Article
Information
The Lichenologist , Volume 44 , Issue 3 , May 2012 , pp. 419 - 440
Copyright
Copyright © British Lichen Society 2012

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