Calonectria ilicicola Boedijn & Reitsma, Reinwardtia 1: 58 (1950)  Fig. 5

MycoBank number: MB 293933; Index Fungorum number: IF 293933; Facesoffungi number: FoF 11342;

Micromorphology: Associated with stem rot of Cassia surattensis. Telemorph: Perithecia 400–700 × 350–550 µm, solitary, red-brown, subglobose to ovoid, perithecial walls rough. Asci fusiform with 8-spored. Ascospores 40–50 × 4–8 µm (x̄ = 45 × 6 µm, n=50), hyaline, fusoid, straight to slightly curved, 1-septate, constricted at septum. Anamorph: Macroconidiophores septate, hyaline. Primary branches of conidiogenous apparatus 15–25 × 4–6 μm; secondary branches aseptate, 10–20 × 4–6 μm; tertiary branches 10–15 × 3–6 μm; each terminal branch producing 2–4 phialides, 10–15 × 3–6 μm. Veslcles 7–12 μm diam, sphaeropedunculate. Macroconidia 40–65 × 4–8 μm (x̄ = 54 × 6 μm, n=50), cylindrical, straight, 1–3 septate. Megaconidia and microconidia not observed.

Culture characteristics: Colonies on MEA fast growing at 25 ℃, growth rate 11.2 mm/d (n = 5), circular, producing abundant white aerial mycelium; reverse red brown.

Material examined: China, Guangdong province, Guangzhou city, Cassia surattensi Burm. F., (Fabaceae), 30th March 2019, Y.X. Zhang (dried cultures ZHKU 21-0008 and ZHKU 21-0009), and living culture, ZHKUCC 21-0011, ZHKUCC 21-0012.

Notes: Two isolates obtained in this study developed a clade with Calonectria ilicicola with 70 % in MP and 0.97 in BYPP supports in phylogenetic analyses. Morphologically, ascospores and macroconidia of our isolates are similar to those of Ca. ilicicola described by Lombard et al. (2010), while microconidia are not observed in this study. Here we introduce the isolate from Cassia surattensis as Ca. ilicicola based on phylogenetic analyses and morphological analyses.

Figure 5. Calonectria ilicicola (ZHKUCC 21-0011: New host record) a–b: Culture characteristics on MEA after 7 days (a, upper view; b, reverse view); c–e: Ascomata; f–g: Asci; h–i: Ascospores; j–k: Veslcles; l–n: Macroconidia; Scale bars: c–e = 100 μm; f = 50 μm; g–n = 10 μm.