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Phylogenetic RAxML tree based on analysis of a combined SSU, LSU, ITS, tef1-α, and rpb2 dataset. Maximum likelihood (ML) and maximum parsimony (MP) equal to or higher than 70% and Bayesian posterior probabilities (PP) equal to or greater than 95% are shown at nodes. New species in this study is in blue.
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A new monotypic coelomycetous genus, Loculosulcatispora (type species L. thailandica) is introduced in Pleosporales from woody litter in Thailand. Phylogenetic analysis of combined non-translated loci (SSU, LSU, ITS) and protein-coding regions (tef1-α, rpb2) shows the genus is a distinct lineage in Sulcatisporaceae. Loculosulcatispora is distinguis...
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... a final ML optimization likelihood value of (-23729.752746). Estimated base frequencies were as follows: A = 0.240592, C = 0.257473, G = 0.270880, T = 0.231055; substitution rates AC = 1.231089, AG = 2.804850, AT = 1.068828, CG = 1.036841, CT = 6.085785, GT = 1.000000; gamma distribution shape parameter α = 0.465094. In the phylogenetic tree ( Fig. 1) Li et al. (2020). Loculosulcatispora is similar to Botryosphaeria, Ciliosporella, Cornutispora, Melanops, Neodermea, Neodidymelliopsis, Neofusicoccum, Phacidium and Sakireeta in having multi-locular pycnidia. However, Loculosulcatispora differs by its conidial characteristics. In the phylogenetic analysis Loculosulcatispora clusters in ...
Citations
... Approximately 17,000 vascular plant species have been recorded in Yunnan, and the fungal population (including lichens) in a given area is estimated to be about six times larger than that of vascular plants based on plant abundance (Hawksworth 1991(Hawksworth , 2001Zhang and Lu 2017), however, despite this vast potential, only a small fraction of these fungal species have been documented so far. The rich fungal diversity in Yunnan has become a focal point for mycologists, with a particular surge in interest in recent years (Yang 2005;Sun et al. 2017;Feng and Yang 2018;Luo et al. 2018;Pem et al. 2019;Dong et al. 2020;Wanasinghe et al. 2020Chaiwan et al. 2021;Ren et al. 2020Ren et al. , 2021aRen et al. , 2021bRen et al. , 2022Bao et al. 2023). Notably, the exploration of asexual morph species has been steadily increasing due to heightened activities in studying microfungi across diverse terrestrial and aquatic ecosystems (Lu et al. 2018;Liu et al. 2019;Wijayawardene et al. 2022;Ren et al. 2021c;Bao et al. 2022). ...
... Taxonomic investigations using combined morphology and phylogeny expand the knowledge of microfungi on woody litter in Yunnan Province, particularly the discovery of several new taxa from Dothideomycetes, including Anteagloniaceae, Didymosphaeriaceae, Hysteriaceae, Hermatomycetaceae, Phaeoseptaceae, and Sulcatisporaceae (Ren et al. 2020(Ren et al. , 2021a(Ren et al. , 2021b(Ren et al. , 2021cWanasinghe et al. 2022). In this paper, we report a new saprobic species of Queenslandipenidiella, highlighting that Queenslandipenidiella is likely to be associated with various hosts and habitats and increasing the information on host association and ecological distribution of Periconia. ...
... Sulcatisporaceae not long ago based on the asexual morph characters of the typified species L. thailandica (Ren et al., 2020). Subsequently, Loculosulcatispora hongheensis was reported on dead woody litter in Yunnan, China, with the description of teleomorph (Wanasinghe et al., 2022). ...
... In addition, the phylogeny indicates that this isolate is in the clade of genus Loculosulcatispora (Supplementary Figure 4). However, our isolate has larger conidiomata, locule, conidiogenous cells, and conidia when compared with the holotype of L. thailandica (MFLU 20-0440) (Ren et al., 2020). Furthermore, there are obvious base-pair differences, viz. ...
... In addition, the type of species of the genus H. velutinum is associated with the dead plant material of the families Adoxaceae, Aquifoliaceae, Asteraceae, Berberidaceae, Betulaceae, Brassicaceae, Cornaceae, Ebenaceae, Fabaceae, Fagaceae, Juglandaceae, Lamiaceae, Magnoliaceae, Menispermaceae, Platanaceae, Poaceae, Polygonaceae, Rosaceae, Tiliaceae, and Ulmaceae, including submerged wood in terrestrial ecosystems Farr and Rossman, 2022). The genus Loculosulcatispora was introduced by its anamorphic characteristics by Ren et al. (2020), and the teleomorphic characteristics were first described with the discovery of L. hongheensis (Wanasinghe et al., 2022). It comprises two fungi, which are saprobic species in dead wood or twigs of unidentified plants and currently reported in Thailand and China. ...
In Sichuan province, walnuts, consisting of Juglans regia , Juglans sigillata , and the hybrid J. regia × J. sigillata , are commercially important edible nuts, and J. regia is the most widespread plant. To date, the diversity and distribution of fungi inhabiting on Juglans have not received enough attention, although there have been studies focusing on pathogens from fruit and stem. In order to update the checklist of fungi associated with Sichuan walnuts, a survey on fungi associated with the three Juglans species from 15 representative regions in Sichuan was conducted. In this article, ten fungi distributed in two classes of Ascomycota (Dothideomycetes and Sordariomycetes) were described based on morpho-molecular analyses, and two novel species, Neofusicoccum sichuanense and Sphaerulina juglandina , a known species of Ophiognomonia leptostyla , and seven new hosts or geographical records of Cladosporium tenuissimum , Diatrypella vulgaris , Helminthosporium juglandinum , Helminthosporium velutinum , Loculosulcatispora hongheensis , Periconia byssoides , and Rhytidhysteron subrufulum were included. Morphological descriptions and illustrations of these fungi are provided.
... BLAST searches using the BLASTn algorithm were performed to retrieve similar sequences from GenBank (http://www.ncbi.nlm.nih.gov, accessed on 29 December 2021) and relevant publications [36,37]. The collection/strain numbers for these sequences (Table 1) are presented in the corresponding phylogenetic trees (Figures 1 and 2). ...
... The ascospores are overlapping uni-to bi-seriate, hyaline, fusiform with acute ends, 1-septate, smooth-walled, surrounded by a thick irregular mucilaginous sheath. Anamorph: see Ren et al. [36]. ...
... The species in this family are mostly restricted to terrestrial habitats excluding Neobambusicola strelitziae, which was found from the coastal region (Eastern Cape Province) of South Africa [58]. The conidia of anamorphs of Sulcatisporaceae can vary from hyaline, aseptate or septate (Anthosulcatispora, Loculosulcatispora, Neobambusicola, Pseudobambusicola), to pigmented phragmo-conidia (Sulcatispora) or muriform conidia (Magnicamarosporium), with or without striation [36,46,56,[58][59][60]. The anamorph of Parasulcatispora is yet to be discovered. ...
In the course of investigating the systematics of woody litter micromycete associates in Yunnan Province, China, we found one new species in Phaeoseptaceae, one new genus and three new species in Sulcatisporaceae from 16 specimens collected (ten collections of ascomycetous teleomorphs, four collections of hyphomycetous and two collections of coelomycetes anamorphs) from Ailaoshan, Chuxiong, Diqing, Honghe, Kunming, Lancang, Mengla and Yuxi in Yunnan Province. These taxonomic novelties were recognized with the aid of morphological comparisons and phylogenetic analyses of multiple gene sequences (non-translated loci and protein-coding regions). Pleopunctum menglaense sp. nov. is accommodated in Phaeoseptaceae (Pleosporales) based on its hyphomycetous anamorph, which is characterized by superficial sporodochia on the host surface, macronematous, mononematous, cylindrical, unbranched, aseptate, hyaline and smooth-walled conidiophores, monoblastic, terminal, hyaline conidiogenous cells, hyaline, muriform α conidia, and brown, muriform β conidia with tri-lobed wing like basal cells. Kazuakitanaka gen. nov. (type: K. yuxiensis) is introduced in Sulcatisporaceae (Massarineae, Pleosporales) for a saprobic ascomycete with teleomorphic and anamorphic (coelomycetous) features. The teleomorph possesses globose to subglobose ascomata with acentric ostiole, a peridial wall of textura angularis to textura prismatica, cylindric-clavate, pedicellate asci with an ocular chamber, and 1–2-septate, hyaline, fusiform, guttulate ascospores with a distinct mucilaginous sheath. The anamorph features pycnidial conidiomata, phialidic, ampulliform to cylindrical, hyaline conidiogenous cells and ampulliform to cylindrical, one-to-three-septate, hyaline, guttulate conidia. Loculosulcatispora was known only from its anamorph of L. thailandica. We observed the teleomorph of Loculosulcatispora hongheensis sp. nov. and amended the generic description of Loculosulcatispora accordingly. Loculosulcatispora hongheensis is characterized by globose to subglobose ascomata with a central ostiole, a peridial wall of textura angularis to globosa, branched, septate, pseudoparaphyses, clavate asci with a short pedicel and a minute ocular chamber and hyaline, fusiform, 1-septate ascospores with a thick irregular mucilaginous sheath. This study provides some insights into the diversity of fungi on dead woody litter in terrestrial habitats.
This study documents the morphology and phylogeny of ascomycetes collected from karst landscapes of Guizhou Province, China. Based on morphological characteristics in conjunction with DNA sequence data, 70 species are identified and distributed in two classes (Dothideomycetes and Sordariomycetes), 16 orders, 41 families and 60 genera. One order Planisphaeriales, four families Leptosphaerioidaceae, Neoleptosporellaceae, Planisphaeriaceae and Profundisphaeriaceae, ten genera Conicosphaeria, Karstiomyces, Leptosphaerioides, Neoceratosphaeria, Neodiaporthe, Neodictyospora, Planisphaeria, Profundisphaeria, Stellatus and Truncatascus, and 34 species (Amphisphaeria karsti, Anteaglonium hydei, Atractospora terrestris, Conicosphaeria vaginatispora, Corylicola hydei, Diaporthe cylindriformispora, Dictyosporium karsti, Hysterobrevium karsti, Karstiomyces guizhouensis, Leptosphaerioides guizhouensis, Lophiotrema karsti, Murispora hydei, Muyocopron karsti, Neoaquastroma guizhouense, Neoceratosphaeria karsti, Neodiaporthe reniformispora, Neodictyospora karsti, Neoheleiosa guizhouensis, Neoleptosporella fusiformispora, Neoophiobolus filiformisporum, Ophioceras guizhouensis, Ophiosphaerella karsti, Paraeutypella longiasca, Paraeutypella karsti, Patellaria guizhouensis, Planisphaeria karsti, Planisphaeria reniformispora, Poaceascoma herbaceum, Profundisphaeria fusiformispora, Pseudocoleophoma guizhouensis, Pseudopolyplosphaeria guizhouensis, Stellatus guizhouensis, Sulcatispora karsti and Truncatascus microsporus) are introduced as new to science. Moreover, 13 new geographical records for China are also reported, which are Acrocalymma medicaginis, Annulohypoxylon thailandicum, Astrosphaeriella bambusae, Diaporthe novem, Hypoxylon rubiginosum, Ophiosphaerella agrostidis, Ophiosphaerella chiangraiensis, Patellaria atrata, Polyplosphaeria fusca, Psiloglonium macrosporum, Sarimanas shirakamiense, Thyridaria broussonetiae and Tremateia chromolaenae. Additionally, the family Eriomycetaceae was resurrected as a non-lichenized family and accommodated within Monoblastiales. Detailed descriptions and illustrations of all these taxa are provided.
The Greater Mekong Subregion (GMS) is known as a diverse geographic landscape and one of the richest biodiversity hotspots in the world with a high fungal diversity. Collections were carried out in terrestrial habitats to determine the diversity of woody litter fungi in the GMS, with an emphasis on northern Thailand and the Yunnan Province of China. Morphological characteristics and multigene phylogenetic analyses of combined SSU, LSU, ITS, and tef1-α supported the placement of the new isolates in the family Didymosphaeriaceae. The phylogenetic affinities of our isolates are illustrated through maximum likelihood and Bayesian inference analyses. Seven species of woody litter fungi were identified, comprising a new monotypic genus, Septofusispora; five novel species (Chromolaenicola sapindi, Dictyoarthrinium thailandicum, Karstenula lancangensis, Septofusispora thailandica, and Spegazzinia jinghaensis); and new host records of two species (Austropleospora archidendri, and Montagnula donacina). Furthermore, this study provides a synopsis of the Montagnula aff. donacina species based on their morphological characteristics, which can be useful in the species-level identifications in this genus.
This paper provides an updated classification of the Kingdom Fungi (including fossil fungi)
and fungus-like taxa. Five-hundred and twenty-three (535) notes are provided for newly introduced
taxa and for changes that have been made since the previous outline. In the discussion, the latest
taxonomic changes in Basidiomycota are provided and the classification of Mycosphaerellales are
broadly discussed. Genera listed in Mycosphaerellaceae have been confirmed by DNA sequence
analyses, while doubtful genera (DNA sequences being unavailable but traditionally
accommodated in Mycosphaerellaceae) are listed in the discussion. Problematic genera in
Glomeromycota are also discussed based on phylogenetic results.
This paper provides an updated classification of the Kingdom Fungi (including fossil fungi) and fungus-like taxa. Five-hundred and twenty-three (535) notes are provided for newly introduced taxa and for changes that have been made since the previous outline. In the discussion, the latest taxonomic changes in Basidiomycota are provided and the classification of Mycosphaerellales are broadly discussed. Genera listed in Mycosphaerellaceae have been confirmed by DNA sequence analyses, while doubtful genera (DNA sequences being unavailable but traditionally accommodated in Mycosphaerellaceae) are listed in the discussion. Problematic genera in Glomeromycota are also discussed based on phylogenetic results.
