SOP for Post Entry Quarantine Inspection - Plant quarantine India

Final DocumentGovernment of IndiaMinistry of Agriculture(Department of Agriculture & Cooperation)Directorate of Plant Protection, Quarantine & StorageN.H.IV., Faridabad-121001Standard Operating Procedures forPostentry Quarantine InspectionCopyNo:_____Document Approved by __________________(Signature/Name/Stamp/ Date)Document Issued by:_________________________(Signature/Name/Stamp/ Date)

SOPs for Postentry Quarantine InspectionSection-0 Table of Contents Page 1 of 1December 2007Table of ContentsSection TopicPagenumber0. Table of Contents 2- 200. Control of Document 3- 51. Introduction 6-142. Receipt & Registration of Application for approval of PEQ Facilities 15-173 Technical Assessment of PEQ Facilities 18-264. Certification of PEQ Facilities 27-295. Quarantine Inspection/Release of Plants/Plant material and other regulated 30-35articles at the port6. Postentry Quarantine Inspection of plants/plant material and other 36-39regulated articles grown in approved facility.7. Laboratory Testing/Virus Diagnosis 40-438. Detection of Pests & Action taken 44-469. Notification of Non-compliance and Emergency Action 47-4810. Reporting & Monitoring of PEQ Inspections 49-5111. Appeal & Revision 52-5212. De-notification of inspection authorities/Action against persons for noncompliance53-54with PEQ13. Pest Monitoring & Sanitary Practices 55-5514. Document Management & Record Control 56-5715. Training 58-5816. Communication, Auditing & Review 59-63Appendix-I: List of important plant species/quarantine pests and postentryquarantine requirements64-762

SOPs for Postentry Quarantine InspectionSection-00 Control of Document Page 1 of 3December 2007Control of Document1. Document issue and revisionThis document issue and revison is controlled by the Dte of Plant Protection, Quarantine & Storage(NPPO), N.H.IV., Faridabad-121001 (Tel: 0129-2413985; fax:2412125; e-mail: ppa@nic.in). As andwhen a section of this document is revised, the revised section is issued in its entirety together with arevision number, identifying the new issue status and the issue date of each section. The revisedsections are automatically issued to each of this document copy holders listed in Section 2 of ‘Controlof Document’:Upon the receipt of a revised section, this document copy holder should remove the obsolete sectionand replace it with revised section together with revision numberSection Title IssueDate of IssueRevisionDate ofNumberNumberRevision0. Table of Contents 100. Control of Document 11. Introduction 12. Receipt & Registration of Application for PEQ 1Facilities3 Technical Assessment of PEQ Facilities 14. Certification of PEQ Facilities 15. Quarantine Inspection/Release of plants/plant 1material and other regulated articles at the Port6. Postentry Quarantine Inspection of plants/plant 1material and other regulated articles grown inapproved facility.7. Laboratory Testing/Virus Diagnosis 18. Detection of Pests & Action taken 19. Notification of Non-compliance and Emergency 1Action10. Reporting & Monitoring of PEQ Inspections 111. Appeal & Revision 112. De-notification of inspection authorities/Action 1against persons for non-compl iance with PEQ13. Pest Monitoring & Sanitary Practices 114. Document Management & Record Control 115. Training 116. Communication, Auditing & Review 13

SOPs for Postentry Quarantine InspectionSection-00 Control of Document Page 2 of 3December 2007This issue status table identifies the latest issue of all section of this document, which has beenprovided to all document copy holders listed in section 2 of ‘Control of document’.This document copy holders are responsible for ensuring all sections of this document are at thecorrect issue status prior to use. The document approval authority as evidenced bysignature/date/stamp of approving authority and document issuing authority as evidence bysignature/date/stamp of issuing authority will be placed on title page and approval of revision sectionindicated against the revision number in the issue table above.2. Document distributionThis document distribution and subsequent revisions distribution are controlled and issued by theDirectorate of Plant Protection Quarantine and Storage, Faridabad. This document is issued to all thosenotified Inspection Authorities (SAUs/ ICAR Institutes), where activities and processes described inthis document is undertaken.This document is issued on a controlled copy basis. The only copies of this document that arepermitted are those held by the copy holders identified in the table below. This ensures that whenchanges to this document are made, all copy holders receive those changes.This document is currently issued to the following copy holders:DocumentCopy NoCopy HolderContact Address4

SOPs for Postentry Quarantine InspectionSection-00 Control of Document Page 3 of 3December 2007DocumentCopy NoCopy HolderContact Address5

SOPs for Postentry Quarantine InspectionSection-1 Introduction Page 1 of 9December 20071.1.Scope/Purpose:This document provides guidance and describes the standard operating procedures for certification offacilities for growing imported plants/plant material including transgenic plant material and otherregulated articles such as biological control agents/beneficial organisms and living modified organismsunder postentry quarantine and inspecting the same to ensure free from quarantine pests and otherregulated non-quarantine pests.The purpose of this document is to facilitate adoption of standard operating procedures by all theinspection authorities notified by the Ministry of Agriculture (Department of Agriculture &Cooperation)/PQOs (with PEQ responsibilities) for undertaking certification of postentry quarantineinspection facilities as well as conducting postentry quarantine inspection of growing plants and otherregulated articles under the supervision of concerned inspection authority/ PQOs (with PEQresponsibilities) in accordance with phytosanitary requirements specified under the Plant Quarantine(Regulation of Import into India) Order, 2003 and amendments issued there under to prevent theintroduction and spread of destructive pests that affects plants and other regulated articles such asbiological control agents and beneficial organisms.1.2.Definitions & Terms:Additional declaration A statement that is required by an importing country to be entered in PhytosanitaryCertificate and which provides specific additional information pertinent to thephytosanitary condition of a consignment.Biological control A natural enemy, antagonist, competitor or other organism used for pest controlagentBulbs & Tubers A commodity class for dormant underground parts of plant intended for planting(includes corms and rhizomes).CertificateAn official document which attests to the phytosanitary status of any consignmentaffected by phytosanitary regulations.Commodity A type of plant, plant product, or other article being moved for trade or other purpose.Compliance procedure Official procedure used to verify that a consignment complies with statedphytosanitary requirements.Consignment A quantity of plants, plant products and/or other regulated articles being moved fromone country to another and covered by a single phytosanitary certificate (aconsignment may be composed of one or more lots).Country of origin (of a Country where the plants were grown.consignment of plants)Country of origin (of Country where the regulated articles were first exposed to contamination by pests.regulated articles otherthan plants and plantproducts)6

SOPs for Postentry Quarantine InspectionSection-1 Introduction Page 2 of 9December 2007FieldFumigationGermplasmImport PermitInspectionInspectorIPPCInternational Standardfor PhytosanitaryMeasures (ISPM)Inspection authorityIsolated conditionA plot of land with defined boundaries within a place of production on which acommodity was grown.Treatment with a chemical agent that reaches the commodity wholly or primarily ingaseous stage.Plants intended for use in breeding or conservation programmesOfficial document authorizing importation of a commodity or of a biological controlagent in accordance with specified phytosanitary requirements.Official visual examination of plants, plant products or other regulated articles todetermine if pests are present and/ or to determine compliance with phytosanitaryregulations.A trained technical staff assigned with the responsibility of inspection/sampling ofconsignments of plants/plant products and other regulated articles to ensure free frompests.International Plant Protection Convention, as deposited in 1951 with FAO in Romeand as subsequently amended.An international standard adopted by the conference of FAO, the interim commissionon phytosanitary measures or the commission on phytosanitary measures establishedunder IPPC.Any institute/research organization notified by the Ministry of AgricultureDepartment of Agriculture & Cooperation) , for the purpose of certification ofpostentry quarantine facilities and conducting of inspection of growing plants andother regulated articles under postentry quarantine.A place where imported material is grownLotNational PlantProtectionOrganization (NPPO)OfficialPestPhytosanitaryCertificateA number of units of a single commodity, identifiable by its homogeneity ofcomposition, origin etc., forming part of a consignment.Official service established by a government to discharge the functions specified bythe IPPC.Established, authorized or performed by a National Plant Protection Organisation.Any species, strain or biotype of plant, or pathogenic agent, injurious to plants orplant products.Certificate patterned after the model certificates of IPPC.7

SOPs for Postentry Quarantine InspectionSection-1 Introduction Page 3 of 9December 2007PhytosanitarycertificationPhytosanitaryregulationPlantsPostentryQuarantine (PEQ)Quarantine PestRegulated articleUse of phytosanitary procedures leading to the issue of a Phytosanitary Certificate.Official rule to prevent the introduction and/ or spread of quarantine pests or to limitthe economic impact of regulated non-quarantine pests including establishment ofprocedures for phytosanitary certification.Living plants and parts thereof, including seeds and germplasm.Growing of plants in isolation for any specified period in a glass-house, afacility, area or nursery and /or holding of biological control agents andbeneficial organisms under contained facility.A pest of potential economic importance to the area endangered and not yet presentthere, or present but not widely distributed and being officially controlled.Any plant, plant product, storage place, packaging, conveyance container, soil andany other organism, object or material capable of harbouring or spread of pestsdeemed to require phytosanitary measures, particularly, where internationaltransportation is involvedRegulatedquarantine pestSeedsTreatmentnonA non quarantine pest whose presence in plants for planting affects the intended useof those plants with an economically unacceptable impact and which is thereforeregulated within the territory of the importing contracting party.Seeds for planting or intended for planting and not for consumption or processingOfficial procedure for the killing, inactivation, or removal of pests or for renderingpests infertile or for devitalization.Visual ExaminationThe physical examination of plants, plant products, or other regulated articles usingthe unaided eye, lens, stereoscope or microscope to detect pests or contaminantswithout testing or processing8

SOPs for Postentry Quarantine InspectionSection-1 Introduction Page 4 of 9December 20071.3. References:Destructive Insects & Pests Act, 1914 and Amendments issued there underGlossary of Phytosanitary Terms, ISPM 5 (2006), FAO, Rome.Guidelines for Inspection, ISPM 23 (2005), FAO, Rome.Import Inspection Manual, PQ-12, (1995), Dte of PPQS, Fardiabad-121001, IndiaInternational Plant Protection Convention, 1997, FAO, RomePlant Quarantine (Regulation of Import into India) Order, 2003 & Amendments issued there under.1.3.Requirements:1.3.1. Legal Authority:The Import Quarantine Inspection Unit of PQ Division of Dte of PPQS (NPPO) will have legalmandate and administrative authority for control and supervision of postentry inspection activitiesperformed by the Inspection Authorities (State Agricultural Universities/ICAR Institutes) notified bythe Ministry of Agriculture (Department of Agriculture & Cooperation) under the Plant Quarantine(Regulation of Import into India) Order, 2003 and amendments issued there under and the PlantQuarantine Officers (with PEQ responsibilities) authorized by the Plant Protection Adviser of Dte ofPPQS from time to time. The The Dte of PPQS (NPPO) will have the legal power for its actions andimplement safe guards against conflicts of interest and decisions taken by the Inspection Authority inrelation to postentry quarantine inspection including approval and certification of PEQ facilities. ThePlant Protection Adviser have the legal power to hear the appeal made by the aggrieved importeragainst the decision taken by the Inspection Authority and call for the records relating to any casepending before the Inspection Authority for the purpose of satisfying itself as to the legality orpropriety of any decision passed by that authority and may pass such order in relation thereto, as itthinks fit as per the provisions contained in the above said PQ Order, 2003.1.4.2. Management Responsibility:The Dte of PPQS (NPPO) will be overall responsible for:? management of national import quarantine regulatory system that ensures that all therequirements including entryquarantine inspection, certification of PEQ facilities; inspection ofplants/plant material and other regulated articles growing under postentry quarantine and theirclearance as per the legislative and administrative requirements are met with? Will designate a senior level technical officer with competency to head the Import QuarantineInspection Unit of PQ Division of Dte of PPQS (NPPO) to closely monitor the activitiesrelated to import quarantine inspection including both entryquarantine inspection and postentryquarantine inspection of plants/plant material and other regulated articles.9

SOPs for Postentry Quarantine InspectionSection-1 Introduction Page 5 of 9December 2007? identify the duties and line of communication of all personnel involved in entryquarantineinspection and postentry quarantine inspection responsibilities in relation to import? ensure that adequate trained and skillful personnel and resources are available both with theinspection authorities and /or the PQO entrusted with the responsibility of PEQ for undertakingfollowing functions:!.5. Resources:- maintenance of information on current phytosanitary requirements of PEQ inspection;- production of operational guidelines/procedures/instructions to ensure that thephytosanitary regulation requirements specified under the PQ order are met with;- inspection and testing of consignments and other regulated articles grown underpostentry quarantine;- identification of organisms found during entry-quarantine inspection/postentryquarantine inspection of consignments and other regulated articles- Approval and certification of postentry quarantine facilities- Reporting of PEQ inspection- Issue PEQ clearance/destruction certificates- document storage and retrieval- training- dissemination of information related to approval and certification of PEQ facilities- notification of non-compliance with phytosanitary requirements and emergency action.1.5.1 Trained & Qualified Staff:The Import Quarantine Regulatory System will have adequate, skilled and trained manpower toefficiently handle the volume of consignments being processed for import inspection includingpostentry quarantine inspection. The Import Quarantine Inspection Unit of PQ Division of Dte ofPPQS (NPPO) will decide the number of technically trained/qualified manpower to be required at eachplace, and periodically review the requirements of human resources and capacity building and trainingrequirements (operational as well as specialized) in consultation with notified inspectionauthorities/PQOs (with PEQ responsibilities) and after evaluating their technical capacities andcapabilities and infrastructure facilities for performance of entry inspection/postentry quarantineinspection activities.10

SOPs for Postentry Quarantine InspectionSection-1 Introduction Page 6 of 9December 20071.5.2. General Facilities:The general facilities for the Import Quarantine Inspection Unit of PQ Division of Dte of PPQS(NPPO) will include office space for the head of the unit and the administrative secretariat withtelephone, fax, computer with internet facility and a dedicated server for management of nationalimport quarantine inspection (including postentry quarantine inspection) database and relatedinformation and communication links with notified inspection authorities/PQOs (with PEQresponsibilities) including on-line reporting.The general facilities for the notified inspection authorities/ PQOs (with PEQ responsibilities) shouldinclude an exclusive office space for the inspection authority/PQO, which is provided with dedicatedtelephone, fax, computer with internet facility for PEQ activity and general laboratory facilities forentomological/nematological/plant pathological work. In addition should have laboratory facilitiesespecially for virus testing using sero-diagnostic (ELISA/DIBA) /molecular diagnostic (RT-PCR/NASH) protocols. An insect-proof screen house/glass house with double door entry facility isrequired for carrying out virus indexing.1.5.3. National Phytosanitary Database:The Import Quarantine Inspection Unit of PQ Division of Dte of PPQS (NPPO) will provide all theoffice of the notified inspection authorities with the requisite software and establish links for on-linereporting of activities related to postentry quarantine viz., approval and certification of postentryquarantine facilities; inspection/release of plants/plant material and other regulated articles for growingunder post entry quarantine inspection; intimation to DIA; PEQ inspection/release and /or destructionof plants /plant material and other regulated articles, pest -interceptions and all the technicalpersonnel of inspection authorities/PQS (with PEQ responsibilities) will be trained to familiarize withthe software application and its use and computerized issuance of certification of PEQ facilities andsubmission of PEQ inspection reports to the Import Quarantine Inspection Unit of PQ Division of Dteof PPQS (NPPO).National Phytosanitary Database will contain effective communication links with all the notifiedinspection authorities and up-to-date data on name, designation, contact address (Mail/telephone/fax/email).National Phytosanitary Database will also contain the information on regulated pests of concernincluding their presence and geographical distribution, the biology, detection and diagnostic protocols,identification of the pest and the appropriate phytosanitary action to minimize the risk.11

SOPs for Postentry Quarantine InspectionSection-1 Introduction Page 7 of 9December 20071.5.4. Equipments:1.5.4.1. Office Equipments:The offices of notified inspection authorities will have the following essential equipments for PEQwork.? Telephone (dedicated)? Fax? Computer with Internet Facility (broadband connectivity/leased line)? UPS (Inverter)? Printer1.5.4.2. Inspection Equipments:? Field lense? Pen knife? Secatuer/pruning shears? Camel hair brush/aspirator? Measuring/marking tape? Soil scoop? Specimen vials (shoulder type) (containing 70% alcohol and /or 3% formalin or empty)? Digital Camera? Heavy duty trash bags? Plastic bags (self-sealing type)? Paper bags? Stapler with pins? Marking pens? Labels/Tags? A role of surgical cotton? Paper towels/tissue napkins? Field note book? Thermo cool box with ice pack12

SOPs for Postentry Quarantine InspectionSection-1 Introduction Page 8 of 9December 20071.5.4.2. General Laboratory Equipments:The inspection authorities should have following equipment for laboratory diagnosis of pests such asinsects/mites; nematodes; fungi & bacteriaEntomology? Insect storage cabinets/mounting boards? Soft X-ray Scanner & Film Developer? Steriobinocular microscope fitted with Image grabberNematology? Nematode Extraction Unit? Fenwick can? Baerman funnel? Sieve set? Steriobinocular microscope? Compound Binocular Microscope? Microscope accessories (slides/cover slips; ocular/stage micrometers; camera lucida)Plant Pathology:? Laminar flow? BOD Incubator? Autoclave? Hot air oven? Digital top pan balance? Analytical Balance? Hot Plate with Magnetic Stirrer? pH meter? Haemocytometer? Inoculation loop or needle? UV fluorescent lamp? Distilled Water Unit? Deep freezer (-20C)? Compound trinocular microscope fitted with Photomicrographic Equipment? Vaccuum cleaner? A set of laboratory chemicals (for preparation of media/stains/reagents etc.)? A set of laboratory glassware (beakers, conical flasks, measuring cylinders, pipettes, Petridishes, test tubes, etc.)13

SOPs for Postentry Quarantine InspectionSection-1 Introduction Page 9 of 9December 2007? Microscope accessories (slides/cover slips; ocular/stage micrometers; camera lucida)1.5.4.3. Special Equipment s for Molecular Diagnosis of Bacteria/VirusesThe following special equipments are required for molecular diagnosis of bacteria and viruses? Tissue grinder? Micropipettes (varying volumes)? Micro plates (for ELISA Test)? ELISA kit (reader, washer, reagents)? Nitrocellulose membrane (for DIBA test)? Immuno-diagnostic reagents (Specific Antisera/Enzymes/substrate/buffers)? PCR? PCR Tubes? Horizontal Gel Electrophoresis Unit with power pack? Hybridization Oven? Gel Documentation Unit with printer? Micro-centrifuge & Effendorf tubes? Nucleic Acid Chemicals (Specific Primers/TAQ DNA Polymerase/buffers/stains etc)? Rnase Kit14

Section-2SOPs for Postentry Quarantine InspectionReceipt & Registration of Application for Approval ofPEQ FacilitiesDecember 2007Page 1-3 of 32.1. Receipt of Application:2.1.1. The importer or his authorized agent will apply in prescribed format (Annexure-2A) along withattached documents (diagrammatic design of the facility/SOPs) to the concerned Inspection Authority(i.e. SAUs/ICAR Institutes) for certification of PEQ facility for growing imported plants and plantmaterial and other regulated articles under confinement sufficiently in advance of import.2.1.2. The technical staff attached to the concerned inspection authority will receive the application and issuean acknowledgement slip.2.2. Verification of Application2.2.1. The technical staff of concerned Inspection Authority will scruitinize the application and attacheddocuments (diagrammatic design of the facility/SOPs), whether the information provides is correct andcomplete.2.2.2. If application is incomplete, he will inform the importer to provide necessary information and correct thedeficiencies in the application..2.3. Registration of Application:2.3.1. The technical staff will register the correct and complete application and assign a registration numberand record the particulars in a register (Annexure-2B) maintained by the inspection authorities forcertification of PEQ facilities and submit the same to the concerned inspection authority.2.4. Scheduling of site visit:2.4.1. The inspection authority will draw a schedule of site visit by a team of at least two experts for technicalassessment of PEQ facilities established by the importer to ensure that they are established inconformity with the criteria laid down under Section-3 of this document and communicate to theimporter concerned..15

Annexure-2A:Application for Certification/Renewal of Postentry Quarantine Inspection Facilities1. Name/Address of the Applicant (Mailing Address/Telephone/Fax/Mobile/E-mail):2. Registration No./Date:2. Type of facility: ( ) Open field; ( ) glass house; ( ) Screen house;( ) Polyhouse and ( ) Others:________________(specify)3. Location of Facility (Village/Taluk/Mandal/District/State):4. Name of Facility Operator & Contact Details (Telephone Number)5. No. of Units/ Extent of Facility (Floor Area/ Potting Space)6. Type of Plants/Plant Material and /or other Regulated Articlesintend to import/Quantity (Nos)/Date by which intend to import &port of entry, where applicable.7. Is the application made for the first time for approval &( ) First time: ( ) Renewalcertification? (If the application is made for renewal, pleaseindicate certificate numb er/date of certification and also attachoriginal copy of certificate issued)8. A brief description of facility (Enclose the diagrammatic sketch/plan of the facility). Use separate sheet.9. Date on which the Facility was established10. Any Additions/Modifications carried out to the Existing Facility. If‘Yes’ give brief account of additions/modifications.Yes/No11. Is the application made by the applicant is rejected/refused for Yes/Noapproval & certification at any time? If ‘yes’ give reasons12. Whether any standard operating procedures (SOPs) in place for the Yes/Nooperation of the facility, including, record keepingpest monitoring & sanitation practices. If ‘yes’ attach a copy ofSOPs)13. Particulars of trained staff operating the Facility (Name/type oftraining/jobwork/experience):14. Any additional informationDeclarationI hereby declare that the information furnished above is complete and correct to the best of my knowledge and belief.Date:_________________Place:__________________________________________________(Signature/Name/Stamp of Applicant/Date)For Office (Accreditation Unit) UseCheck list Status Scrutinized by Action by IA Applicant ResponseApplication Yes NocompleteFacilityYes Noplan/diagramFacility SOPs Yes NoFinal Action Taken:By:_________________________________________(Signature/Name/Designation of Inspection Authority16

Annexure-2B.Register for Certification of Postentry Quarantine FacilitiesRegistrationNumberDate Applicant (Name/Address Type of Facility Location(Place/Taluk/District/State)Extent offacility(No ofunits/size/capacity)Type of plantspecies intendto grow &QuantityWhethercertificateissued orrejectedRemarks, ifany/Sign of IA

SOPs for Postentry Quarantine InspectionSection-3 Technical Assessment of PEQ Facilities Page 1 of 9December 20073.1. A team of nominated experts will undertake the site visit for on-spot technical assessment of thefacility on scheduled date and time as communicated earlier.3.2. The technical assessement of the facilities will include physical examination of the facility to verifythat the facility meet the criteria laid down under Annexure-3A and also examination of recordsmaintained at the facility, procedures practiced and testing the skill competency of technical staffoperating the facility.3.3. The team of nominated experts, at the end of visit, will prepare an assessment report in theprescribed format separately for open field quarantine facilities (Annexure-3B) and closedquarantine facilities (Annexure-3C) together with the comments/recommendations and correctiveactions, if any suggested. The assessment report will be duly signed by the facility owner/operatorand the leader of the inspecting team.3.4. If any corrective actions are required, the inspection authority will communicate the owner of thefacility the corrective actions required to be undertaken and the time schedules for correctiveaction to be implemented.3.5. The facility will be re-inspected by the same nominated experts after the scheduled time period toverify the corrective actions are undertaken3.6. If satisfied, the team of experts will recommend the facilities for approval and certification by theinspection Authority3.7. If required corrective actions are not satisfactorily undertaken, the inspection authority may rejectthe application for certification of facilities and inform the facility owner as per the formatprescribed in Anexure-4B.

SOPs for Postentry Quarantine InspectionSection-3 Technical Assessment of PEQ Facilities Page 2 of 9December 2007Annexure-3ACriteria for Approval and Certification of Postentry Quarantine Facilities1. Open Field Quarantine Facilites:The open field quarantine facilities such as isolated nurseries/growing of crops is confined togermplasm, seed crops, bulbs/tubers of flowers & oilpam nurseries and forest nurseries raised fromimported seed/ propagating plant material. The open field quarantine facilities must meet the followingrequirements for approval and certification1.1. Location/Isolation:The field should be distinctly located in an offshore island for maintaining germplasm of plantation crops such ascoconut. For annual seed crops/bulbs & tubers etc., of flowers, the facilities should be established in an isolatedfield, which is distinctly marked, The minimum isolation distance from similar cropped area should be of 500metres.1.2. Security of the Facility:The field should have a barbed wire fencing or similar material with a lockable gate to prevent anyunauthorized person trespassing through the area with a clear sign board indicating “PostentryQuarantine Area-Entry is denied without Permission” and watch and ward.1.3. Soil treatment:The soil in the field should be appropriately treated either by steam sterilization or fumigation orsolarization to render pest-free. The treated soil should be tested especially for freedom from soil-borneparasitic nematodes/fungi before planting.1.4. Trap crop/barrrier:The field should be bordered around with high density polythene film up to a height of 10 ft without anyopenings or gaps and /or raised around with 3-4 rows thick barrier crop such as Daincha & Sesbania toserve as an insect barrier.1.5. Watering:The water used for irrigation should be of good quality and appropriately treated to render pest free. Thefield should be irrigated through a drip irrigation system and or furrow or basin or bed irrigated and nooverhead irrigation (sprinkler) system should be used.19

SOPs for Postentry Quarantine InspectionSection-3 Technical Assessment of PEQ Facilities Page3 of 9December 20071.6. Drainage:The field should be properly leveled to prevent water logging and with good drainage facility.1.7. Field sanitation;The field should be free from weeds and residues of previous crop and proper. Arrangements fordisposal of infested/infected plant material1.8. Documentation:The facility should have standard operating procedures in place for the operation of facility (includingrecord keeping, pest monitoring and plant protection practices)1.9. Staff training:The staff should be adequately trained in field operations, pest monitoring, plant protection practices andrecord keeping.2. Closed Quarantine Facilites (Glass house/Screen house/Polyhouse)The closed quarantine facilities such as glass houses/screen houses/poly houses are required for growingimported high risk ornamental and fruit plant species including tissue culture plants. The facilitiesestablished must meet the following criteria for approval and certification.2.1. Double Door Entry:The facility should have a double door entry with outer solid door, which is lockable and inner screeneddoor fitted with a door closure to prevent any entry/escape of pests and the entry to the facility should berestricted. The double door entry porch is provided with a foot both and a basin containing disinfectantfor hands and foot disinfection2.2. Flooring:The facility should have concrete flooring for easy cleaning and washing or soil floors should becovered with impermeable membrane. The drainage holes should be appropriately covered with screento prevent entry of hitch hiking pests and rat-proof. There should be small water trough around theexterior of the facility to act as ant barrier.20

SOPs for Postentry Quarantine InspectionSection-3 Technical Assessment of PEQ Facilities Page 4 of 9December 20072.3 Structural Design of Facility:The structural design of the facility may vary depending on the plant species and location of the facilityand climatic conditions of the area in which the facility is located, either it is with angular roof or bowshaped roof or ground to ground tunnel house. The structural frame should comprise of tubular columnsand angular trusses made of mild galvanized steel for easy maintenance.2.4. Roof/Wall Cladding:The facility should have roofs/walls cladded with either poly carbonate or polythene or covered with aninsect proof screen or mesh. The all side screened houses should have adequate protection on the top bya sliding roof of polythene film against rain in heavy rain fall areas. In case of poly houses with top sideopenings the openings should be screened with appropriate size of mesh to prevent entry of hitch hikingpests such as Lepidopteran moths. The maximum height of the house upto the trusses should not bemore than 3.5-4 m. However the length/breadth of the house will very upon the requirement. In case oflarger houses or modular houses there should .be a clear partition to segregate the plant material.2.5. Vector-Proofing of facility:All the vents/openings to the facility should be covered with a screen of 40-60 meshes for linear inchmade of good quality material either of stainless steel/phosper bronze or nylon to prevent entry ofvectors such as aphids, mealy bugs, thrips & white flies.2.6. Lighting/Heating/Cooling/Shading Requirements:The facility should have adequate lighting to facilitate proper growth of the plants. If the facility locatedin temperate areas, it should have adequate heating with heating ducts are evenly distributed to provideuniform heating, which is thermostatically controlled. The facility should have adequate cooling systemeither by cool cell pad system/desert cooler and /or a fan coil unit, which is thermostatically controlled.The facility should have either internal/external shade netting arrangements for cutting down excesslighting.2.7. Watering/Misting;The facility should have adequate watering facility through automatic drip irrigation for irrigating thebeds or potted plants and over head misting facilities for rejuvenation of cuttings/hardening of tissueculture plants.2.8. Cleaning & washing of pots:The facility should have a separate area for cleaning/washing/disinfecting pots and other containers usedfor planting.21

SOPs for Postentry Quarantine InspectionSection-3 Technical Assessment of PEQ Facilities Page 5-9 of 9December 20072.9. Potting area:The facility should have separate potting area for filling up of pots with growing media and storing ofpots and containers.2.10. Soil treatment:The facility should have adequate equipment for sterilization of soil and other organic media, forgrowing of plants, by vapour heat or by chemical disinfectants. The treated soil should be testedespecially for freedom from soil-borne parasitic nematodes/fungi before planting.2.11. Incinerator:The facility should have an incinerator for destruction of affected plant material or a separate area fordisposal of affected plant material by burning.2.12. Fixed/Sliding benchesThe facility should have either fixed or sliding benches for raising the potted plants above the groundwith adequate isle space of ¾-1m for easy movement of men and material.2.13. Documentation:The facility should have standard operating procedures in place for the operation of facility (includingrecord keeping, pest monitoring and plant protection practices)2.14. Staff training:The staff operating the facility should be adequately trained.3. Containment Facility:Special containment facilities required for handling biological control agents and beneficial organismsHigh level containment facilities such as filteration of air through spore proof filters are required forhandling GMO’s and transgenic plant material and entry provided with air-curtains and treatment ofdischarges from the facility .22

Annexure-3B.Technical Assessment Report for Certification of Open Field Quarantine Facility1. Name of Facility Owner: 2. Application Reg No/Date:3. Location of Facility (village/Taluk/District/State)4:Contact Address (Postal/Telephone Number/Fax/ Mobile/E-mail)5. Assessed by i)_______________________________(Name & Designation of Expert)ii)______________________________________(Name & Designation of Expert)6. Date of Visit7. Field Number/Extent of Area8. Extent of quarantine area Marked9. Name of Plant Species intend to be grown10.Details of previous crops grown11 Details of AssessmentCriteria Yes No CommentsFacility is distinctly located in an offshore island and/or isolated from similar/related crop species up to adiameter of 500 to 1000 mThe field is enclosed around with barbed wire fencingwith lockable gate to prevent any unauthorized entryThe field is bordered around with a high densitypolythene film up to a height of 10 ft with out anyopenings or gaps and /or raised around with 3-4 rowsthick barrier crop such as Daincha & Sesbania toserve as insect barrier.Suitable sign board such as ‘Postentry QuarantineArea- No Entry W ithout Permission’ is displayednear the entry gate to prevent tress passes...The field is located in a elevated area and properlyleveled with adequate drainage conditionsThe water used for irrigating the field is of goodquality and appropriately treated to render it pest –free.Soil beds are appropriately treated by pasteurizationor fumigation to render pest-freeThe field is watered through a drip irrigation systemand or furrow or basin or bed irrigated and nooverhead irrigation (sprinkler) system used.The field is free from weeds and refuse of previouscrop, if any23

Security sufficient to prevent unauthorized accessDocumentationStandard operating procedures in place for theoperation of the facility (including, record keepingpest monitoring & sanitation practicesStaff training and competency satisfactoryFacility Meets the requirements as listed aboveCorrective Action, if any required to be under taken/Time scheduleFacility Owner’ssignature/Date_______________________________Inspection Authority’s signature/Date: ________________________Corrective Actions have been undertaken& verified:_______________________(Inspection Authority’s Signature/Date)24

Annexure-3C.Technical Assessment Report for Certification of Closed Postentry Quarantine Facility(Glasshouse/Screen house/Polyhouse)1. Name of Facility Owner: 2. Application Reg No/Date:3. Location of Facility (village/Taluk/District/State)4:Contact Address (Postal/Telephone Number/Fax/ Mobile/E-mail)5. Assessed by i)_______________________________(Name & Designation of Expert)ii)______________________________________(Name & Designation of Expert)6. Date of Visit7. Type of Closed Quarantine Facility ( ) Glasshouse; ( ) Screenhouse; ( )Polyhouse8. No. of Units/ Floor space9. Name of Plant Species intend to be grown10. Details of AssessmentCriteria Yes No Commentsvector-proof facilityAdequate double door entranceEntrance foot-bath/hand wash basin with disinfectantpresentAll the gaps from the external to internalenvironment are properly sealedAppropriate temperature, light and humidity controlsexist at the facilityProper misting facilities for tissue culturehardening/acclimatization of transplantsFacility (and all containers) disinfected and free ofplants, debris or soilSoil-less medium used and pest free and /or treatedsoil is used.Separate facility for pottingWater used is of good quality and appropriatelytreated to render pest freeSliding and raised benches for growing tissueculture plantsSoil floors covered with protective membraneSecurity sufficient to prevent unauthorized access25

DocumentationStandard operating procedures in place for theoperation of the facility (including, record keepingpest monitoring & sanitation practicesStaff training and competency satisfactoryFacility Meets the requirements as listed aboveCorrective Action, if any required to be under takenTime scheduleFacility Owner’s signature/Date_______________________________Corrective Actions undertaken &verified:Inspection Authority’s signature/Date: _________________________________________________(Inspection Authority’s Signature/Date)26

SOPs for Postentry Quarantine InspectionSection-4 Certification of PEQ Facilities Page 1-3 of 3December 20074.1. Preparation of certificate:4.1.1. The concerned Inspection Authority, after satisfying with the recommendations of inspectionteam, will prepare a certificate of approval of PEQ facilities as per format prescribed inAnnexure-4A.4.2. Issue of Certificate:4.2.1. The certificate of approval will be issued in triplicate viz., ‘original’ for the facility owner;‘duplicate’ forwarded to the officer-in-charge of concerned plant quarantine station at concernedport through which the plants/plant material and /or other regulated articles are intended toimport and the ‘triplicate’ is maintained as office copy in the case folder.4.3. Validity of Certificate:4.3.1. The validity of certificate will be for a maximum period of six months for open field quarantinefacilities for annual seed crops, bulbs/tubers of flowers/forest tree species (with the exception ofoil palm which is for 18 months); one year in case of polyhouse/screen (nylon) house facilitiesand 3 years in the case of glass (polycarbonate)/screen (stainless steel/phosperbronze) housefacilities4.4. Renewal of certification:4.4.1. The certificates issued for open field quarantine will be further renewed for a maximum period ofsix months subject to necessary re-inspection of facilities and there after no renewal will begranted.4.4.2. The certificates issued for closed quarantine facilities such as polyhouses/screen (nylon) housesare renewed annually subject to necessary re-inspection of facilities4.4.3. The certificates issued for glass (polycarbonate) houses/ screen (stainless steel/phosperbronze)are renewed after every two years but will be subject to annual verification4.4: Rejection/Cancellation of Certification:4.4.1. The inspection authority will issue rejection/cancellation of certification of PEQ facilities in theformat prescribed in Annexure-4B, if he is satisfied after inspection/verification, that facilitieswill not meet the criteria laid down in Section-3 of this document or that the facility does notcomply with the postentry quarantine regulatory requirements specified under the PlantQuarantine (Regulation of Import into India) Order, 2003 and the amendments issued thereunder, as the case may be under intimation to the concerned PQO at the designated port throughwhich intend to import/imported and the Dte of PPQS (NPPO).27

Annexure-4A___________________________________________________________(Name of the Institute/Organization)Certificate No:Date of issue:Valid up to:CERTIFICATE OF APPROVAL OF PEQ FACILITYIn accordance with the provisions of Clause 11 of the Plant Quarantine (Regulation of Import into India) Order,2003 issued under sub-section (1) of the Section 3 of the Destructive Insects and Pests Act, 1914, andamendments issued there under, I hereby certify that the following facility has been inspected and approved forgrowing of imported consignment of plants/ planting materials as described below, under postentry quarantine,as per the Standard Operating procedures (SOPs) established by the Dte of PPQS (NPPO) and subject to thefollowing terms and conditions.Date: ______________Place: _______________________________________________(Seal)(Signature/Name of Inspecting Authority)1. Name and address of the Importer: :2. Location of PEQ facility (Village/ Taluk/District/State): :3. Type of facility (Open field/Glasshouse/Screenhouse/Polyhouse):4. No. of Units & size of each Unit :5. Total capacity (No. of propagating Units/ potting space) :6. Name of plant species intended to be grown ::Terms & Conditions of certification:1. The Original Certificate of Approval shall be displayed in a prominent place at the Facility and a copy of thecertificate shall be forwarded to the authorized officer of PQS of concerned port through which the plants/plantmaterial described above are intend to import to facilitate issue of import permit.2. The Certificate of approval is valid only up to the date indicated unless otherwise renewed.3. The holder of certificate shall hold valid license issued by the Director of Agriculture of the concerned State4. The certificate is valid will apply at least one month in advance of the date of expiry for renewal and no certificatewill be renewed after its expiry.5. The certificate of approval granted shall be liable to be withdrawn/cancelled, if the holder of certificate is involvedin making false records or not abide by the instructions given by the Inspection Authority during the course ofinspection of growing plants under postentry quarantineEndorsements:Revalidated/suspended/cancelled on ___________ by___________Revalidated/suspended/cancelled on____________by___________Copy to:28

To:______________________________________________________________________________(Name/Address of Facility owner)Annexure-4BRef No:_______________________DatedRejection/Cancellation of Certification of PEQ FacilitiesIt is hereby informed that the PEQ facilities described here under have been inspected by a tem of expertsnominated by the undersigned and considered that the same will not meet the criteria laid down for certification under theStandard Operating Procedures (SOPs) established by the Dte of PPQS (NPPO). Therefore your application is rejected forapproval of Certification of Postentry quarantine facilities for growing imported plants/plant material and /or otherregulated articles due to the reasons given below:Date:___________Place:___________(Signature/Name of Inspection Authority)2. Location of PEQ facility (Village/ Taluk/District/State): :3. Type of facility (Open field/Glasshouse/Screenhouse/Polyhouse):4. No. of Units & size of each Unit :5. Total capacity (No. of propagating Units/ potting space) :6. Name of plant species intended to be grown :7. Reasons for Rejection/Cancellation of certification:Copy to:29

Section-5SOPs for Postentry Quarantine InspectionQuarantine Inspection/Release of plants/plantmaterial and regulated articles at the PortDecember 2007Page 1-6 of 65.1. Quarantine Inspection/ Release of Plants/Plant material & Regulated Articles at the Port:5.1.1. The importer will secure an import permit for import of seeds/plants/plant material forpropagation sufficiently in advance from PQO at designated port through which intend toimport.. The importer and/ or his agent will file an application in the format prescribed under PQOrder, 2003 with the PQO at designated port for import inspection and quarantine clearance ofplants/plant material and or other regulated articles along with a copy of import permit,phytosanitary certificate (original), certificate of approval of PEQ facilities, an undertaking forgrowing imported plants/plant material and/or other regulated articles (Annexure-5A), otherrelevant documents and pay the necessary PEQ inspection fees, just prior to arrival ofconsignment at the designated port. The importer and/ or his agent will ensure Custom’sforwardal of consignment to the PQ station immediately upon the arrival at the designated port.5.1.2. The PQO at designated port, immediately up on receipt at quarantine station, will undertakeinspection of consignment and after necessary verification of documents accompanied theconsignment and after ensuring free from pests will grant provisional release for growing underpostentry quarantine in the format prescribed under PQ Order, 2003 and /or destruction ofconsignment in the event of interception of a quarantine pest, under intimation to Dte of PPQS.If any new organism is intercepted, he will determine the pest status of the organism and takeappropriate action as considered necessary in consultation with Dte of PPQS (NPPO) and untilsuch time the consignment will be detained at the cold storage facility at the PQS or at airport.5.2. Intimation of Inspection Authority:5.2.1. The PQO at designated port, immediately after granting provisional clearance, will intimate theconcerned inspection authority in the format prescribed at Annexure-5B along with a copy ofundertaking to grow imported plants/plant material and or other regulated articles (Annexure-5A). He will provide a copy of crop specific pest information (pest description/diagnosticprotocol) and post entry quarantine inspection requirements notified from time to time in aprescribed format (Annexure-5C). A list of important plant species, quarantine pests andpostentry quarantine requirements are presented at Appendix-I,5.2.2. The PQO at designated port will issue postentry quarantine tags/labels (Annexure-5D) in twonumbers per each individual species/variety imported. These tags will be forwarded to theinspection authority along with intimation letter to facilitate tagging the plants (one on the firstplant of specified species/variety and the other on end plant), while growing under post entryquarantine. The tags/labels to be printed in orange green/colour with the relevant informationclearly marked on the backside of tag/label and should be laminated or waxed to prevent wetdamage. The PEQ tagging of the plants will facilitate easy identification of species/variety andcharacterization of individual shipments and further distinguish the plants that are held under30

postentry quarantine from other plant material. In case of tissue cultures imported for in-vitromultiplication no PEQ tagging is applicable.Annexure-5A.To: ____________________________________Undertaking for Growing Plants and Plant Material under Postentry Quarantine under the Supervision ofInspection Authority._____________________________________________________________________I/we, M/s___________________________________________________________________________give the following undertaking in respect of a consignment of ________________________________ intend tobe imported vide Import Permit No._____________________________ dated____________________________through _____________________________ and that upon release to grow in an approved postentry quarantinefacility under the supervision of notified inspection authority (IA)and or/PQ officer authorized by the PlantProtection Adviser to the Government of India, Dte of PPQS agree to the following terms and conditions:1. I/we shall grow the entire consignment of Imported plant material (as described above) in an approvedpostentry quarantine facility/isolated nursery located at the village______________________________________ of taluk _______________________ of Dist._________________________ of ___________________ State.2. I/we undertake to intimate the concerned IA/PQO about the date of sowing/planting of seeds/ propagatingplant material, percentage of germination, seedling mortality and plant protection measures if any adoptedetc., within one month of sowing/planting and thereafter at regular intervals.3. I/we shall provide all the facilities to IA/PQO for undertaking postentry quarantine inspection ofseedlings/plants raised out of imported seed/plant material at the above locality.4. I/we undertake to maintain the nursery records/registers relating to the receipt of seed/plant material,germination/planting records, plant protection measures undertaken, record of various operations in thenursery and produce the same before inspecting team for necessary scrutiny.5. I/we agree to undertake necessary plant protection measures as advised by the inspecting team from timeto time.6. I/we undertake not to give/donate/distribute any part of the consignment without the written clearancefrom the IA/PQO duly authorized by PPA in this behalf.7. I/we hereby undertake to provide at our cost free transport facility for inspection team during the visit tothe nursery/PEQ facility for undertaking inspection of plants/plant material.8. I/we hereby agree to abide by the decision of inspection authority/PQO to destroy whole or part ofconsignment or any seedling/plant material, which in his/their opinion found infected/infested orcontaminated by a quarantine pest/pathogen, in the manner prescribed by him/them and to furtherundertake appropriate measures for decontamination of tools and garden equipment, soil etc., thereof onemergency basis.9. I/we hereby undertake to bear the cost of destruction of affected plant material under the supervision ofinspection IA/PQO.31

10 I/we agree to maintain basic inspection tools like hand lens, field lens or illuminated magnifier, surgicalspirit, dissection box, absorbent cotton, screw caped glass vials, labels etc., for the purpose of carrying outinspection.11. I/we agree to the condition that no liability lies with IA/PQO towards loss/damage caused to any plantmaterial/destruction of the same in the event of infection/infestation by a quarantine pest/pathogen.12. I/we agree to the condition that in the event of non-compliance with the terms and conditionstated above, I/we forfeit the future claim and right for issue of permit.Date: ____________________________________________Name & Signature Importer/AgentPlace & Address:___________________________________________________________________________N.B.: The Importer/Agent is required to submit the above undertaking in duplicate to PQO at designated port, theduplicate copy which will be forwarded to respective inspection authority (IA) along with intimation letter to IA.32

Annexure-5B.To:_____________________________________________________________________________________________________( Name/Address of Inspection Authority/PQO)Ref No:________________Date:__________________Intimation of Release of Consignment of plants/ plant material for growing under postentryquarantine under the supervision of Inspection Authority (IA)/PQOSir,The following consignment of plants/ seeds imported for planting/sowing has been inspected by thisoffice and recommended for release to grow under PEQ facility at ________________for a period of__________________. The IA/PQO shall inspect the above consignment at the time of sowing/ planting andat periodical intervals and submit the reports of PEQ inspections at the end of final inspection for this officerecord under intimation to Plant Protection Adviser, Directorate of Plant Protection, Quarantine and Storage,Faridabad.Date:_______________Place:________________________________________(Signature/Name of Officer-in-Charge)1. Name of Commodity :(species/variety)2. Quantity (Nos. & Weight) :3. Country of Origin :4. Import Permit No. & Date :5. PSC No. & Date :6. Name & Address of Importer :7. Customs Ref. No. & Date :8. Date of Inspection :9. Date of Release :10. Inspection Remarks :Copy to:1. Importer, with instruction that the packages of the consignments shall be opened immediately uponreceipt at the site only in the presence of IA/PQO and follow necessary instructions and guidelines fromIA/PQO from time to time.2. Director of Horticulture/ Agriculture for information33

Annexure-5CPost-entry Quarantine Inspection Information Sheet1. Name of the Plants/Plant Material(Common/Scientific Name):2. Country of origin:3. Details of Regulated Pests of Concern:Name of the Pest Biology of Pest DiagnosticScientific Common Symptoms* MorphologicalDescription ofPathogen*Protocol*Attach photoes, if any. Use extra sheet, if necessary.4. Post entry Quarantine Inspection Requirements:5. Any additional information:6. Name/Signature/Designation of PQOat designated port:34

Annexure-5DPostentry Quarantine TagWARNINGThese plants are held underPOSTENTRY QUARANTINEKeep the Tag on the Plant. If any of the Plant Die,immediately notify the Inspection Authority/PQO.No Plants or any part of Plant (including dead plants)described on reverse side of this tag should not beremoved/disposed/distributed/multiplied withoutwritten permission ofInspection Authority/PQOPQ Refer.No:_________ Date of Import___________Name of Plant Species/Variety:__________________Quantity (Nos):_______________________________Origin: ______________________________________Designated Portthrough, which Imported:_______________________35

Section-6SOPs for Postentry Quarantine InspectionPostentry Quarantine Inspection of plants plant materialand other regulated articles grown in an approved facilityDecember 2007Page 1 of 46.1. Inspection of Open field cultivated crops:6.1.1. The annual seed crops grown in open field quarantine under isolated/protected condition will beinspected at least twice. The first inspection will be when the seedlings are about one month oldand the second inspection will be when they are at flowering stage. During the first inspectioninformation on seedling mortalities will be recorded as well as any incidence of virus/downymildew infection should be recorded and the affected plants will be sampled for virus testing andall suspected plants will be rogued out and destroyed by incineration. During the secondinspection at flowering stage organ-specific infections such as ergots, bunts and smuts arerecorded.6.1.2. In the case of bulbs/tubers of flowers the initial inspection will be carried out at the time oftransplanting in the field to ensure that only healthy bulbs are transplanted and the incidence ofbulb rots caused by fungi, bacteria & nematodes should be recorded and the specimens ofaffected bulbs and soil samples are collected for laboratory testing. The second inspection will becarried out, when the seedlings are about 30 days old to record virus infections and further thesamples of affected plants collected for virus testing and all the suspected plants will be roguedout and destroyed.6.1.3. During crop inspections, the inspection authority should ensure that a minimum isolationdistance. of 500 metres are maintained from similar related crop species as specified and apolythene barrier is provided around the crop or barrier crop is raised around up to a height toserve as insect barrier against pest such as aphids, thrips and whiteflies etc., as specified; thepests are monitored in the field by way of sticky traps and necessary sanitary practices..6.2: Inspection of plants/plant material grown in glass house/screen house6.2.1. The imported foliage plants are inspected at the time of transplanting into pots inside the glasshouse/screen house/polyhouse facility to ensure that they are free from insect infestation andvisual symptoms for fungal, bacterial and viral infection. If any plants are sowing virus infectionthey are sampled for immediate virus testing and all suspected plants should be immediatelysegregated and isolated to prevent virus spread. If the test results are positive, the affected plantswill be rogued out and destroyed and the plants will be re-inspected after a fortnight to cull outthe virus infections that escaped the attention during the first visit and the final inspection will becarried after 45 days of planting.6.2.2. In the case of dormant cuttings/buddings, .the first inspection will be carried one month afterbudding or transplanting to facilitate rejuvenation of cuttings/buddings and the second inspectionwill be carried out after 60 days of planting.36

Section-6SOPs for Postentry Quarantine InspectionPostentry Quarantine Inspection of plants plant materialand other regulated articles grown in an approved facilityDecember 2007Page 2 of 46.2.3. In case of fruit plant species, such as citrus, grapes, apple, pear, plums etc., the first inspectionwill be carried out at the time of transplanting in pots and thereafter all the virus suspected plantswill be indexed on a susceptible variety to facilitate virus expression. The indexed plants will beinspected as per the indexing schedules drawn by the inspection authority in consultation withDte of PPQS (NPPO) and ICAR crop specific research institutes.. .6.3. Inspection of tissue culture plants:6.3.1. The imported mother stock culture grown in-vitro in isolation at the accredited tissue culturelaboratory will be inspected by the specified inspection authorities and each and every culturewill be tested virus-free as per established protocols before the same is permitted for initiationin to tissue culture production. If any virus infection noticed the entire mother culture will bedestroyed by incineration under intimation to Dte of PPQS (NPPO).6.3.2. If tissue-culture raised plants/ex-washed plants are imported the same will be inspected by thespecified institutes notified under PQ Order, at the time of planting in the insect-proof tissueculture hardening facility such as screen house, poly house, and glasshouse, the same is sampledbatch-wise and tested virus-free as per established protocols, before the same is permitted forrelease for initiation into tissue culture production. If any virus infection noticed the entire batchof plants will be destroyed under intimation to Dte of PPQS (NPPO).6.4. Inspection of other regulated articles:6.4.1. The imported cultures/organisms of biological control agents and beneficial organisms includingGMO’s will be held under quarantine at the high level containment facilities established by theauthorised institutes in accordance with guidelines established by the Dte of PPQS (NPPO).6.4.2. If the biological control agents or beneficial organisms are the one, which is already establishedin the country, the same will be inspected by the technical expert to ensure that they are freefrom contamination or infestation/infection by natural enemies and other organisms and will befurther cultured or reared for at least two consecutive life cycles and subsequently tested freefrom the same before according release from postentry quarantine.6.4.3. If the biological control agents or beneficial organisms are introduced newly for the first time,the same will be inspected to ensure free from contamination or infestation/infection by naturalenemies/antagonists and further its impact on other organisms and host specificity is evaluatedunder postentry quarantine conditions before permitting experimental release and which isclosely monitored.37

Section-6SOPs for Postentry Quarantine InspectionPostentry Quarantine Inspection of plants plant materialand other regulated articles grown in an approved facilityDecember 2007Page 3-4 of 46.5. Postentry Quarantine tagging of plants:6.5.1. The inspection authority at the time of inspection will ensure that the plants grown under postentry quarantine should be placed with PEQ tag one on the first plant of individualspecies/variety and the second at the end plant of the same species/variety. These tags will beforwarded to the inspection authority by the PQO at the designated port at the time of provisionalrelease of plants for growing under post–entry quarantine and will be removed by the inspectionauthority, when the plants are released at the end of postentry quarantine inspection.6.6. Sample forwardal/receipt for laboratory testing ;6.6.1. The sample forwarded for laboratory testing will be appropriately packed in a thermo-cool boxwith ice pack to prevent any escape of pest, sealed and labeled. The sampling label will providedetailed information viz., Reference Number, Name of the Commodity (Species/Variety), Plantparts sampled, Field/GH or SH or PH Unit No., Sample size, Place of inspection, Date ofinspection, Name/Signature of Inspector. The samples will be collected in the presence of facilityowner/operator.6.6.2. The samples on receipt at the office of the inspection authority/PQO (with PEQ responsibility)will be entered in a PEQ inspection register (Annexure-6A). The sample will be divided intotwo portions and one will be issued to the concerned laboratory for testing and the other portionwill be held in a refrigerator or held under cold storage room to prevent spoilage by microbialcontamination for future reference until the laboratory testing is completed.38

Annexure-6A.PEQ Inspection RegisterRef.No.Date (s) ofinspection/samplingName ofFacility/Location/UnitNumberName of theCommodity(Species/variety)PlantpopulationgrownSamplesizeNo ofsamplesdrawnSampledbyTestscarriedoutTestedbyName of Pestdetected(Scientific)Action taken &signature ofIA/PQO

SOPs for Postentry Quarantine InspectionSection-7. Laboratory Testing Page 1 of 4December 20077.1. Laboratory testing by Inspection Authority:7.1.1. The samples on receipt will be tested by concerned laboratory expert according to internationallyestablished or validated diagnostic protocols, where appropriate. If the test results are proved tobe positive for the quarantine pests specified under PQ order, 2003, the same may be brought tothe immediate notice of inspection authority.7.1.2. At the end of laboratory testing the concerned expert will report the test results in prescribedformat (Annexure-7A) to the inspection authority7.1.3. The inspection authority on receipt of test report may verify the test results and the test protocolsadopted and pest identifications and enter in the inspection/testing register or order, if necessary,the repetition of test results to confirm the findings.7.1.4. I f any new organism is intercepted, the identification of the same may got authenticated by anational referral laboratory and its pest status may be evaluated before reporting the same to Dteof PPQS (NPPO)7.1.5. The inspection authority will adopt sero-diagnostic protocols such as ELISA/DIBA andmolecular diagnostic protocols such as RT-PCR and NASH for characterization of virus andbacteria.7.1.6. It should be ensured that a portion of the sample tested for virus infection will be preserved indeep freezer at -80 C as reference sample with appropriate labeling giving reference number fortracing back the sample to consignment imported.7.1.7. The concerned expert will exercise appropriate quarantine precautions to avoid contamination,while handling of virus-affected material, preparation of samples and carrying test and disposalof unutilized portion of sample.7.1.8. If any inoculations of indicator plants required to be performed by the inspection authority toestablish biological identity or indexing, the tests should be strictly confined to vector-proofquarantine glass house/screen house/polyhouse facility under isolated and secured condition toprevent any escape or spread of virus and inoculated hosts should be immediately be destroyedby incineration after recording the test results and taking photographs including electronmicrographs, if any.

SOPs for Postentry Quarantine InspectionSection-7. Laboratory Testing Page 2-4 of 4December 20077.2.. Specimen forwardal for identification by referral laboratory7.2.1. .If the inspection authority/PQOs (with PEQ responsibilities) can not be able to identify the pestor organism detected in imported plants/plant material, he will forward the specimens along withspecimen identification request form (Annexure-7B) to the national referral laboratoryrecognized by the Dte of PPQS (NPPO) under intimation to PQO at designated port and Dte ofPPQS (NPPO).7.2.2.. Insect (belong to the order: Coleoptera, Diptera, Homoptera (except white flies), Hyminoptera,Orthoptera (immature ones), Thysanoptera) and mite (Acarina) specimens collected should bekilled by placing in 70% of alcohol in screw-capped vials and labeled. The larvae of Lepidopterashould be killed by placing in boiled water and gradually cooled and later placed in 70% alcoholin screw-capped vials. The adult insects of Lepidoptera, Orthoptera & Homoptera (white flies)will be killed by placing in insect killing bottle containing cyanide or ethyl acetate and pin adultspecimens on Styrofoam pinning blocks, which are pinned to the bottom of pinning box. TheHomopteran insects such as scales/mealy bugs/psyllids will be killed and preserved along withhost material as dry mounts.7.2.3. The nematode specimens will be extracted from soil, infested plant material using a nematodeextraction unit or standard sieving method or Baerman funnel technique as the case may be. Theextracted nematodes will be transferred to Syracuse glass and examined under steriobinocularmicroscope fitted with base illuminator. The plant parasitic nematode specimens (thread-likeworms with stylet) will be transferred to a vial containing few ml of water and gently heat killed.The heat killed specimens are placed in a fixative such as 3% formalin (1ml of formaldehyde +12 ml of water). However cysts of nematode and mature females of Meloidogyne spp., aredirectly transferred to fixative without heating7.2.4. In case of diseased specimens, the affected plant material will be partially dried and placed inbetween sheets of stiff absorbent paper to keep the diseased area flat and ensure not foldingspecimens, while mailing to the identifier or if not permanent slide mounts or cultures offungi/bacteria isolated from disease specimens will be submitted for identification. However theplants suspected of virus infection, leaves along with branch will be placed in between folds oftissue paper and kept in self sealing plastic bags, which are sealed air-tight to maintain freshnessand turgidity of specimen. The sealed bags are in turn placed in side a bubbled package and sendit by courier to nearest virus testing laboratory.41

Annexure-7ALaboratory Test Report1. Test Report No: 2. Date of Reporting3. Sample Reference Number 4. Date of Reference5. Name of Laboratory/Organisation6. Name of Technical Expert/Contact Number7. Host plant species/variety examined8. plant parts examined9. No of samples tested/size of sample10. Details of testingType of Test Carried out Name of pest/organism detected Description Notes, if anyScientific Name Common name Taxon11. Remarks, if any:Expert)______________________________(Signature/Designation of Laboratory42

Anexure-7BSpecimen Forwardal for identification by Referral Laboratory1. Collection Number: 2. Date of Collection:3. Submitting Organisation:4. Name/Address of the Sender:5. Place of Collection (Name/Address of PEQ Facility/PQStation/Others):6. Reasons for identification:Name of the host species (Common/Scientific) & variety onwhich reported:6. Origin of host:9. Plant Parts affected:10. Category of pest specimen/organism submitted11. Life stage of the pest (Applicable to Insects)12. Type of pest specimen/organism submitted14. Number specimens submitted per each collection:15. Signature/stamp/office seal of the Sender with date:* [ ] roots; [ ] stems; [ ] leaves; [ ] inflorescence; [ ] fruits;[ ] seeds/nuts [ ] others (______)*tick out in appropriate box* [ ] insects; [ ] mites; [ ] nematodes; [ ] fungi; [ ] bacteria;[ ] virus; [ ] others (________)*tick out in appropriate box* [ ] egg; [ ] larvae; [ ] pupae; [ ] adult; [ ] nymphs;[ ] juveniles; [ ] cysts; [ ]others (_______)*tick out in appropriate box* [ ] preserved specimen; [ ] pinned/card board mountedspecimen; [ ] dry specimen with host; [ ] culture; [ ]disease specimen (fresh); [ ] disease specimen (partiallydry); [ ] slide mount; [ ] others (________)*tick out in appropriate box_______________________________________For Identifier Use16. Name & Address of Identifier/Referral Laboratory:17. Remarks of identifier (condition of receipt of specimens)18. Pest Identification (Common/Scientific Name/Taxon):19. Description Notes, if any:Place: ______________Date: _________________________________________________(Signature/Name/Designation of Identifier)Note: This form should be prepared in duplicate by the sender and forwarded to the identifier/referral laboratory along with each collection ofspecimen. The identifier should return the original copy after entering the particulars of the pest identified along with description notes andremarks if any to the sender of the specimen and duplicate copy will be retained by the identifier.43

SOPs for Postentry Quarantine InspectionSection-8 Detection of Pests & Action Taken Page 1-3 of 3December 20078.1. Detection of Regulated Pests:8.1.1. The Inspection Authority/PQO (with PEQ responsibility) on detection of regulated pest will orderthe destruction of the affected consignment of plants/plant material and /or other regulatedarticles held under quarantine/grown under postentry quarantine, either whole or a part of plantpopulation, after taking into account the nature of the pest, level of infection/infestation and levelof containment of its spread/isolation/segregation..8.1.2. Where destruction of any plant population is ordered by the Inspection Authority/PQO (with PEQresponsibility), the importer shall destroy the same in the manner as may be directed by theInspection Authority/PQO (with PEQ responsibility) and under his supervision.8.1.3. The inspection authority/PQO (with PEQ responsibility) will promptly notify the Dte of PPQS(NPPO) regarding the detection of regulated pest in the imported consignment and the actiontaken in the format prescribed in Annexure-8A8.2. Detection of Non-Quarantine Pests:8.2.1. If the pest detected is considered to be of non-quarantine pest, the inspection authority/PQO willadvise necessary plant protection measures to control the pest, before the same is permittedrelease to the importer or his agent.8.3. Detection of New Organism:8.3.1 If any new organism is detected, the inspection authority/PQO will determine the pest status oforganism detected and proved to be potential phytosanitary threat, he will order immediatedestruction of affected population and promptly notify the Dte of PPQS (NPPO) for furthernecessary action in the format prescribed in Annexure-8A8.4. Issue of Release/Destruction Certificate:8.4.1. The inspection authority/PQO at the end of PEQ inspection will issue a release/destructioncertificate (Annexure-8B) to the importer or his agent under intimation to the concerned officerin-chargeof RPQS/NPQS at the designated port through, which the consignment was imported.44

Annexure-8ATo: ______________________________________________________________________________________________________(Name/Address of Dte of PPQS (NPPO)Notifcation No:Date:Notification of Detection of Regulated Pest (s)/New Pest (s) by Inspection Authority/PQOThis is to notify that the following plants/plant material grown in an approved postentry quarantine facility under oursupervision have been inspected/tested and found to be infected/infested by the regulated pest (s)/new pest (s) as describedhere under and there fore whole/a portion of plant population affected by the regulated pest (s)/new pest (s) has beendestroyed by incineration under our supervision:Date:________________Place:_______________Seal__________________________________(Name/Sig./Stamp of Inspection Authority/PQO)1. Name of the Plants/Plant material (Species/Variety)inspected:2. Number of plant population grown inspected:3. Import Ref No./Date:4. Country from which it is imported:5. Port through which imported:5. Name/location of PEQ Facility/Unit:6. Name of regulated pest (s)/new pest (s) (common/scientific name & Taxon) detected:7. Date of Inspection/Testing:8. Tested according to:9. No. of plants affected:10. Action taken:Copy to:_______________________________________________________________________________(Name/Address of PQO at designated port through which plant material has been imported)45

Annexure-8B.______________________________________________________________________________________________________________(Name/Address of Inspection Authority/PQOCertificate No.DateCertificate of *Release and /or Destruction issued by Inspection Authority/PQOThis is to certify that the plants/plant material and other regulated articles described hereunder grown under the approvedfacility described herewith have been inspected by the undersigned and found free from quarantine pests as specified in thePlant Quarantine (Regulation of Import into India) Order, 2003 and amendments issued there under and therefore grantedfinal clearance *and /or a portion or whole of the plants/plant material and other regulated articles have been destroyed dueto reasons given here under.Date:________________Place:________________Note: * strike out, which ever not applicable.Name of Commodity (Plant species/Variety)Quantity grown (No of units).Name/Address of the ImporterSeal_______________________________(Signature/Name/stamp of Inspection Authority/PQO)Import Reference No/Date/PQSFacility (Name/Location)Date of sowing/plantingDate of final clearance and /or destructionReleased Quantity (No of units)Destroyed Quantity, if anyReasons for DestructionCopy to: _______________________________________________________________________________(Name/Address of PQO at designated port)46

Section-9SOPs for Postentry Quarantine InspectionNotification of Non-compliance & EmergencyActionDecember 2007Page 1-2 of 29. Notification of Non-compliance & Emergency Action:9.1. The Dte of PPQS (NPPO) on receipt of information regarding the interception of regulated pest inan imported consignment of plants/plant material will promptly notify the exporting country in theformat prescribed in Annexure-9A., in accordance with ISPM No. 13 (2001): Guidelines for theNotification of Non-compliance and Emergency Action, FAO, Rome.9.2. The notification will be sent to the IPPC contact point or where contact point has not beenidentified, to the NPPO of exporting country, unless bilateral arrangements exist which specify towhom the notification should be sent.9.3. The Dte of PPQS (NPPO) will verify the identification of pest/organism detected in importedconsignment of plants/plant material and or other regulated articled during port inspections and orpostentry quarantine inspection from the concerned PQO/inspection authority and itsauthentication, where required, before notifying the NPPO of exporting country and will advisethe concerned inspection authority/PQO to retain the evidence such as appropriate specimens ormaterial for a period of one year following notification or until necessary investigation has beencarried out.9.4. The significant instances of non-compliance will include:- failure to comply with phytosanitary requirements;- detection of regulated pests (specify)- failure to comply with documentary requirements? absence of phytosanitary certificate (PSC)? uncertified corrections/alternations or erasures in the PSC? incomplete certification? fraudulent phytosanitary certificates- prohibited consignments- contaminated with soil- failure of specified treatment as evidenced by interception of live pest- repeated interception of prohibited consignments/articles in passenger baggages/mails9.5. The emergency action will be taken on the detection in an imported consignment- regulated pests not listed as being associated with commodity from the exportingcountry- organism posing a potential phytosanitary threat.47

Annexure-9ANotification of Non-Compliance & Emergency Action Taken1. Import Reference No: ___________ Dated:___________2. Directorate of Plant Protection, Quarantine & StorageN.H.IV., Faridabad-121001, India5. Name of the Plants/Plantproducts (Species/variety)6. Quantity(Wt/Nos7. PhytosanitaryCertificate orother RefNo/ Date8. Name ofConsignee3. Export Reference No:____________ Dated: __________4. ______________________________________________________________________________________________________(NPPO of Exporting Country)9. Name ofConsignor10. Marks,if any11. Date ofinspection12. Nature of Noncompliance13. Phytosanitary Action Taken:14. Signature/Name/Stamp/ of Authorised Signatorywith date__________________________________(seal of organization)

SOPs for Postentry Quarantine InspectionSection-10 Reporting & Monitoring of PEQ inspection Page 1-3 of 3December 200710. Reporting & Monitoring:10.1. The concerned inspection authority/PQO (with PEQ responsibilities), at the end of PEQ inspectionwill submit a report in prescribed format (Annexure-10A) to the PQO at the designated portthrough which the plants/plant material and other regulated articles have been imported underintimation to Dte of PPQS within 7 days after final inspection. He will also forward a copy ofpostentry quarantine release and /or destruction certificates issued to the importer10.2.The Dte of PPQS (NPPO) will develop and install appropriate software and establishcommunication link with concerned inspection authority/PQO (with PEQ responsibility for on-linereporting of post entry quarantine inspection activities and closely monitor the activities ofinspection authorities to ensure that PEQ activities are effectively performed and the inspectionsand certifications timely carried out

Annexure-10 A (Part-I).(emblem)1. Name/Address of Importer_________________________________________________________________________________________(Name of The Inspection Authority (SAU/ICAR Institutes )/PQOPostentry Quarantine Inspection ReportPEQ Rept. No.Date of Reporting:2. Location of PEQ Facility3. Imported through (Name of designated port)4.PQ Reference No/Date5. Particulars of Plant material released for growing under postentry quarantinePlants/Plant material received for plantingSpecies(a)(b)(c)(d)(e)Variety(f)(g)(h)(i)(j)(k)(l)10. Any additional Remarks, if any:Quantity(Nos)plantedDate ofplantingField No/GH/SH/ PHUnit No.6. Origin :7. Condition on receipt:8. Any insect pest intercepted:9. Diseases intercepted10. Any treatment given:_________________________________(Signature/Name of Inspection Authority/PQO):50

Annexure-10A (Part-II)11. Record of PEQ inspectionsSpecies variety Nos First Inspection Second Inspection Third inspection Follow-up Follow-up(a) Date & findings Date & findings Date & findings Date & findings Date & findings(b)(c)(d)(e)(f)(g)(h)(i)(j)(k)(l)Total No of plants/No of plantsdestroyed, if any12. Any plants are sampled for laboratory testing:13. Inspected by:14. Laboratory testing for pest determination (use additional sheet, if required)Host tested(species/varietyName of pest/organism detectedDescription Notes, if anyType ofTestCarriedoutNo ofsamplesexaminedScientific NameCommonnameTaxonLifestagePest status15. Action Taken in the event of pest detection:16. Final Recommendation for release and /or destruction:Date:________________________________________________(Signature/Name of Inspection Authority/PQO)N.B:- The report will be prepared in triplicate duly signed by inspection authority and submitted in original to the PQO at designated port through which plant materialis imported and duplicate to the Dte of PPQS, N.H-IV., Faridabad-121001 and triplicate retained as office copy by inspection authority within one week after finalinspection.

SOPs for Postentry Quarantine InspectionSection-11 Appeal & Revision Page 1 of 1December 200711.1. Appeal:11.1. An aggrieved importer may appeal to the Plant Protection Adviser against the decision of theinspection authority regarding the destruction of any plant population within 7 days from thedate of communication of the decision giving the grounds of appeal in accordance withprovisions of PQ order, 2003.11.2. The memorandum of appeal should be accompanied by a bank draft in favour of the PlantProtection Adviser and payable at Faridabad, evidencing the payment of fee of Rs. 100/-11.3. The Plant Protection Adviser of Dte of PPQS (NPPO) immediately upon the receipt of appealfrom the aggrieved importer will register the application.11.4. PPA will review the appeal request, previous reports and will evaluate the appeal’s argumentsand call for all the relevant records relating to the case from the inspection authority.11.5. He will schedule a meeting with appellant and the concerned inspection authority/PQO (withPEQ responsibility) after giving them one week notice and chair the meeting along with 2-3experts.11.2. Revision:11.2.1. After hearing the case from both sides and receiving any additional information to counter thearguments, he will close the meeting.11.2.2. After the meeting he will make a decision on the appeal. based on technical considerations andlegal provisions and prepare the proceedings within two weeks after the meeting.11.2.3. If the decision is in favour of the appellant, he will notify the appellant regarding acceptance ofappeal and pass an order against the concerned inspection authority/PQO (with PEQresponsibilities) within one-two days after the preparation of proceedings.11.2.4. If the decision is not in favour of the appellant he will notify the appellant regarding the rejectionof appeal within one-two days after the preparation of proceedings.

Section-12SOPs for Postentry Quarantine InspectionDe-notification of inspection authorities/Actionagainst Importer’s violation of PEQ regulationsDecember 2007Page 1 of 212.1. Denotification of Inspection Authority:The Dte of PPQS (NPPO) may recommend any notified inspection authority to the Ministry ofAgriculture (department of Agriculture & Cooperation for denotification and or stringent action againstPQO, provided it is satisfied that there are reasonable grounds to believe that concerned inspectionauthority/PQO involved in issuing fraudulent certificates without verification of PEQ facilities or issuedrelease certificates without conducting any PEQ inspections or not maintained proper records as perSOPs or caused the destruction of healthy plant material without assigning any scientific reasons afterverification of necessary records and after constituting enquiry into the incident.12.2. Action against Importer’s violation of PEQ regulations:12.2.1. The inspection authority/PQO (with PEQ responsibility) will report any importer’s violation ofpostentry quarantine regulations to the Dte of PPQS (NPPO) under intimation to PQO atdesignated port through which plant material is imported;12.2.2. The activities, which constitute the importer’s violation of postentry quarantine regulationsinclude:- illegal import of propagating plant material without adhering to quarantine regulationsprescribed under PQ Order, 2003 and amendments issued there under- not abide by the decision of inspection authority/ PQOs (with PEQ responsibilities) todestroy whole or part of consignment or any seedling/plant material, which in his/theiropinion found infected/infested or contaminated by a quarantine pest/pathogen, in themanner prescribed by him/them;- removed or disposed or distributed or transferred or multiplied or sold the plant material,while growing under postentry quarantine, without written permission from the inspectionauthority;- caused objection for the entry of notified inspection authority/authorized PQO or theirtechnical experts to the facility for conducting postentry quarantine inspection of importedplants/plant material and taking appropriate samples for laboratory testing;- improper maintenance of PEQ facility and improper handling of material withoutobserving proper quarantine safeguards resulting in escape and spread of quarantine pests;and,53

Section-12SOPs for Postentry Quarantine InspectionDe-notification of inspection authorities/Actionagainst Importer’s violation of PEQ regulationsDecember 2007Page 2 of 2- improper maintenance of records related to the receipt of seed/plant material at the facility,germination/planting records and records of pest monitoring and plant protection measuresundertaken and disposal of plant material;12.2.3. The Dte of PPQS (NPPO) on receipt of report of importer’s violation of PEQ regulations fromconcerned inspection authority/PQOs (with PEQ responsibilities) will immediately orderinvestigation into the cause of violation and in the first instance will issue an official warning ofviolation of postentry quarantine regulations to the specified importer and in repeated instanceswill take stringent action of suspending all future imports by the specified importer.54

SOPs for Postentry Quarantine InspectionSection-13 Pest Monitoring/Sanitary Practices/Quarantine Safeguards Page 1 of 1December 200713.1. Pest monitoring13.1.1. The facility owner or operator will undertake monitoring of pests at weekly intervals throughthe hanging of yellow sticky cards at the crop canaphy level for small insects such as aphids,thrips, whiteflies, leaf minors at the rate of one for every 10 sq. m and pheromone traphs at therate of one for every 100 sq.m area for budworms and record their incidence in a registermaintained for this purpose and also appropriate plant protection measures taken for thecontrol of pests.13.1.2. In the event of any vector population is recorded, he will immediately undertake thoroughinspection of the facility to detect any damages to the screen/polythene and the same may gotimmediately be repaired and any openings got sealed to prevent recurrence.13.2. Sanitary practices13.2.1. The technical staff/workers attached to the facility will adopt sanitary practices, while makingentry to the facility and ensure disinfection of hands and foot and wear sanitized apron andslippers, while working inside the glass house. The workers should avoid smoking andchewing inside the facility.13.2.2 The tools used for handling the plants such as scissors/budding knives/secateures will bedisinfected with 75% alcohol between plants.13.2.3. The facility will be cleaned regularly of any soil spillages and all the plant debris will becollected immediately in a trash bag and will be disposed in a manner advised by theinspection authority/PQO.13.3. Segregation of shipments:13.3.1. Subsequent shipments of plants and plant material from similar origin will be segregated by atleast one metre distance within the same enclosure and labeled13.3.2. Shipments of plants of different origin will be isolated within the same enclosure by a temporarypolythene partition and labeled.55

SOPs for Postentry Quarantine InspectionSection-14 Document Management & Record Control Page 1 of 2December 200714.1. Document Management:14.1.1. The notified inspection authorities/PQOs (with PEQ responsibility) will adopt the standardformats prescribed herewith for postentry quarantine inspection and approval and certification ofPEQ facilities and follow the Standard Operating Procedures for Postentry Quarantine Inspectionestablished by the Dte of PPQS (NPPO) for harmonization of postentry quarantine inspectionactivities to meet the phytosanitary regulations issued under PQ order, 2003 and amendmentsissued there under.14.1.2. The notified inspection authorities/PQOs (with PEQ responsibility) will maintain a technicalfolder to receive and file all the technical information received from the Dte of PPQS (NPPO)related to postentry-quarantine inspection and certification of PEQ facilities; list of regulatedpests; and crop specific inspection standards, diagnostic/testing protocols etc.14.1.3. If any changes to the Standard Operating Procedures or revision of document considerednecessary, the required changes will be communicated by the notified inspectionauthorities/PQOs (with PEQ responsibility) to the Dte of PPQS (NPPO) along with technicaljustification for necessary approval of change and adoption of revision/modification. Thenotified inspection authorities/PQOs (with PEQ responsibility), however, will not make anychanges to the document prescribed herewith or introduce new document without any writtenapproval of Document Approving Authority14.1.4. As and when any modifications/amendments/revision of documents is brought out, the Dte ofPPQS (NPPO) will promptly communicate to all the concerned holders of this document andensure their replacement. The copy holders should ensure that the obsolete documents arepromptly replaced by the revised documents together with revision number to keep it up-to-date.The obsolete documents will be cancelled and filed separately in “obsolete document” folder toprevent confusion or misuse of the document.14.1.5. The inspection authority/PQO (with PEQ responsibility) will ensure that this document is easilyaccessible to inspectors/laboratory technicians/technical experts to facilitate compliance with theStandard Operating Procedures for PEQ Inspection.56

SOPs for Postentry Quarantine InspectionSection-14 Document Management & Record Control Page 2of 2December 200714.2. Record Control:14.2.1. The inspection authorities/PQOs (with PEQ responsibility) will maintain all the records of allactivities related to postentry quarantine inspection of plants/plant material and /or regulatedarticles in each folder in respect of each consignment and separately for certification of PEQfacilities by each facility-wise.14.2.2. Each certification folder should contain the original application received from the facilityowner/operator for certification of PEQ facilities and attached documents, communication ofdeficiencies/schedule of visit, technical assessment report, certificate of approval of PEQfacilities issued and /or rejection/cancellation of certification and renewal of certification andother relevant documents pertaining to the facility.14.2.3. Each inspection folder should contain intimation letter regarding quarantine release ofplants/plant material issued by the PQO at designated port, a copy of undertaking given by theimporter or his agent for growing imported plants/plant material under postentry quarantine, acopy of report of results of postentry quarantine inspection, a copy of certificate of finalrelease/destruction issued by the inspection authority/PQOs (with PEQ responsibility), record ofpest interceptions, pest identification and authentication and other relevant documents pertainingto the consignment).14.2.3. Each certification folder should be arranged registration number-wise for easy retrieval. Also theinspection folder should be arranged consignment (reference number)-wise.14.2.4. The records of pest interception (preserved cultures/specimens/microscopic slides) in importedconsignments of plants/plant material grown under postentry quarantine, diagnostic test protocol,pest identification including, photographs, digital images, microphotographs, gel documents etc.,and authentication reports.14.2.5. The records related to certification of facilities will be maintained for a period of at least three tofive years and the record of inspection of plants/plant material and/or other regulated articles willbe maintained for at least one year and should be able to be retrieved when required. Besidesthis Facility Certification Register, PEQ inspection register are maintained up to date, seriallynumbered and duly certified by the inspection authority/PQO (with PEQ responsibility), as thecase may be The registers will be retained for a minimum period of one year after theircompletion for auditing and verification. .57

SOPs for Postentry Quarantine InspectionSection-15 Training Page 1 of 1December 200715. Training:15.1. Dte of PPQS (NPPO) will review with the inspection authority/PQOs (with PEQ responsibility),identify and record the training needs of the technical personnel in undertaking certification ofPEQ facilities and inspection of growing plants/plant material under postentry quarantine to meetthe phytosanitary regulations issued under the Plant Quarantine (Regulation of Import into India)Order, 2003 and amendments issued there under.15.2. The Dte of PPQS (NPPO) will identify internal/external training needs after taking into accountresources available and prepare training programme and request the concerned recognised institutefor organising the external training. Dte of PPQS (NPPO) will develop appropriate trainingmodules through the consultancy of external experts.15.3. The Dte of PPQS (NPPO) will identify human resources (trainers/training coordinator) andprepare training schedule (Title of Training Work-Shop, Place, Dates (From/To, Trainers &Contact Address of Training Coordinator) for conducting training and budget plan for organizingtraining workshops.15.4. The selected place for conducting operational training-workshop should have comfortable roomwith sitting chairs with tables/desks for 15-20 trainees and the trainers, LCD Projector and screenfor power point presentations and computer facility and printer and white board with marker pensand with drinking water facilities and the space is adequately lighted. Also have access to glass(polycarbonate) house/screen house/poly house facilities for growing plants/plant material forconducting operational training in postentry quarantine activities.15.5. The nominated trainers will organize training workshop on scheduled dates and venue as per thetraining modules approved by the Dte of PPQS (NPPO).15.6. All the personnel with legal responsibilities of approval and certification of PEQ facilities andinspection of plants/plant material growing under postentry quarantine, will be given anoperational training on all activities related to certification of PEQ facilities and carrying out postentry quarantine inspection of plants and plant material. Those involved in virus diagnosis will begiven specialised training in serological and molecular techniques such as ELISA, DIBA, NASH,RT-PCR and C-DNA probes etc., at the Advanced Centre in Plant Virology at IARI, depending onthe requirements.15.7. The operational training will be of minimum of one week duration and is a must for all inspectionauthorities/PQOs entrusted with operational activity responsibilities (certification of PEQFacilities/PEQ inspections). However, the specialised training programmes will be of a minimumof 2-4 weeks duration as may be decided by the above recognised institute which is offering thetraining. The qualified trainees will be issued a training certificate by the Dte of PPQS (NPPO).58

SOPs for Postentry Quarantine InspectionSection-16 Communication, Auditing & Review Page 1 of 5December 200716.1. Communication:16.1.1. The Dte of PPQS (NPPO) will establish appropriate procedures for timely communication torelevant personnel and to the industry concerning changes in:- phytosanitary regulatory requirements;- international/national standards for phytosanitary measures- list of regulated pests-operational procedures16.1.2. The Dte of PPQS (NPPO) will:16.2. Auditing:-liaise with the nominated representatives of NPPO of relevant export contracting party todiscuss the phytosanitary requirements/issues;-establish contact point for the NPPO of exporting country to report cases of noncomplianceand emergency action.-liaise with the relevant Regional Plant Protection Organization and other internationalorganizations to facilitate the harmonization of phytosanitary measures and thedissemination of technical and regulatory information.16.2.1. The Dte of PPQS (NPPO) in consultation with Ministry of Agriculture (Department ofAgriculture & Cooperation) will establish a panel of technical experts (both internal andexternal) for auditing of postentry quarantine inspection activities performed by the variousnotified inspection authorities/PQOs (with PEQ responsibility) to ensure that the standardoperating procedures for certification of PEQ facilities and postentry quarantine inspection ofimported plants/plant material and /or other regulated articles are followed.16.2.2. The Dte of PPQS (NPPO) will establish a schedule of audit and nominate at least two expertsfrom the auditing panel for carrying out the technical audit of postentry quarantine inspectionactivities and intimate the concerned experts one month in advance, to facilitate making travelarrangements under intimation to concerned inspection authority/PQO (with PEQ responsibility).The scheduled audits will be carried out once in every year.16.2.3. Besides the above, unscheduled audits will be organized at least once in a year at a short noticewithout intimating the concerned inspection authorities/PQOs (with PEQ responsibility) toensure compliance with the standard operating procedures for PEQ inspection.59

SOPs for Postentry Quarantine InspectionSection-16 Communication, Auditing & Review Page 2-5 of 5December 200716.2.4. Surveillance audits will be carried out at least once in six months or at such intervals as may bedecided by the auditors to ensure corrective actions are taken and preventive measures areimplemented subsequent to scheduled audting.16.2.5. Such audit inspections will involve the verification of records, verification of certificationrecords, inspection and sampling/testing procedures actually practiced inspection of certifiedfacilities and growing plants, verification of action taken on previous audits and virus testingprotocols and skill competency of technical personnel attached to inspection authorities andverification of nonconformities with postentry quarantine inspections etc.16.2.6. At the end of each audit, an audit report in prescribed format (Annxure-14A) will be prepared bythe auditors in consultation with concerned inspection authority/PQOs (with PEQ responsibility)and submit to the Dte of PPQS (NPPO). The audit report should indicate the non-conformitiesobserved and corrective/preventive action to be taken and time schedules by which the measureswill be implemented to improve the functioning.16.2.7. The concerned inspection authority/PQOs (with PEQ responsibility) will submit the correctiveaction/preventive measures taken report (Annexture-14B), which will be reviewed by the auditorat the time of surveillance auditing and reported to the Dte of PPQS (NPPO).16.2. Review:16.2.1. The Dte of PPQS (NPPO) will periodically review the effectiveness of all aspects of its exportcertification system in consultation with all the notified Inspection Authorities/PQOs nominatedby the Plant Protection Adviser and implement changes to the system if required. Such reviewmeetings will be held annually to discuss the issues and implement corrective actionplans/preventive measures for their recurrence.16.2.2. The Dte of PPQS (NPPO) will establish a procedure for investigating into non-performingInspection Authorities and recommend to the Ministry of Agriculture (Department ofAgriculture & Cooperation) for de-notification.60

1. Name & Address of InspectionAuthority/PQO (with PEQ responsibility)auditedAnnexture-16AAudit (Scheduled) Report.2. Auditees (Name & Designation):2.1. Name/Designation of PEQ Expert:2.2. Technical staff responsible for thePEQ Activity3. Auditing related to the period of4. Date (s) of Auditing: From: To:5. List of Records Audited/Documentsverified:6. Audited by (Name & Designation):7. Details of Auditing reported:7.1 General Comments:7.2 Specific non-conformities observed:S.No.Type of non-conformity observed Frequency Corrective Action/ preventive measures to betaken.61

8. (a) Signature/Name Designation of Auditee(Inspection Authority) with date______________________(b) Signature/Name/Designation of Auditors with date1._____________________2.____________________62

1 Name & Address of InspectionAuthority/PQO entrusted with PEQresponsibility:Annexure-16BAudit (Surveillance) Report2. Date of auditing (surveillance):3. Audited by (Name/Designation):4. Details of audit (surveillance) carried out:S. Type of non-conformity/ observation made byNo. previous auditCorrective Action / preventmeasures undertakenRemarks ofAuditor_______________________(Inspection Authority/PQO)___________________________(Auditor)63

Appendix-IList of important plant species/quarantine pests and postentry quarantine requirementsS. No. Plant species/plantmaterial1. Albizia lebbeck ( Acacia)- plants2. Alstroemeriaspp.(Alstroemeria)3. Anthurium spp & otheraroids (Dieffenbachia,Caladium, Syngonium,Aglaonema,Spathiphyllum, Monstera,Phylodendron)-plants4. Asparagus officinalis(Asparagus)-plants)Quarantine pests(a) Uromycladium tepperianum (Acacia rust)(a) Arabis mosaic virus (hop bare-bine)(b) Freesia mosaic virus(c) Tobacco rattle virus (spraing of potato)(a) Xanthomonas axonopodis pv. Dieffenbachiae (Bacterialblight)(b) Dasheen mosaic virus(a) Phytophthora cryptogea (tomato foot rot)(b) Rhizobium (Agrobacterium) rhizogenes (hairy root)(c) Asparagus virus 1(d) Asparagus virus 2(e) Strawberry latent ringspot virusPostentry quarantine requirementsPostentry quarantine for 45-60 days ininsect proof polyhouse or mesh housewith top covered with polythene sheetfor rain protection. Two to threeinspections, with one at the time ofplanting and the second after 30 daysafter planting and third at 45 daysafter planting. .Postentry quarantine for 45-60 days ininsect proof polyhouse or mesh housewith top covered with polythene sheetfor rain protection. Two to threeinspections, with one at the time ofplanting and the second after 30 daysafter planting and third at 45 daysafter planting. .Postentry quarantine for 45-60 days ininsect proof polyhouse or mesh housewith top covered with polythene sheetfor rain protection. Two to threeinspections, with one at the time ofplanting and the second after 30 daysafter planting and third at 45 daysafter planting. .Postentry quarantine for 45-60 days ininsect proof polyhouse or mesh housewith top covered with polythene sheetfor rain protection. Two to threeinspections, with one at the time ofplanting and the second after 30 daysafter planting and third at 45 daysafter planting. .

5. Arachis spp (ground nut)-seeds/vegetativecuttings/plants(a) Puccinia arachidis (rust)(c) Sphaceloma arachidis (scab)(d) Peanut mottle virus(e) Peanut stripe virus(f) Peanut stunt virus6. Cacti-plants (a) Cactodera cacti (Cactus cyst nematode)(b) Cactus virus X. & 2 (Carlavirus)7. Castanea spp. (Chestnut)-seeds/plants/ plantingmaterial8. Citrus spp (Citrus)-rooted/un-rootedcuttings/budwood/plants9. Cocos nuciferae(Coconut) and otherCoccoidae-seednuts/plants(a) Cryphonectria parasitica (Chestnut blight)-American strain(a) Deuterophoma tracheiphila (Mal secco)(b) Spiroplasma citri (Stubborn or little leaf)(c) Xanthomonas campestris pv. Aurantifolii (Cancrosis B)(d) Citrus tatter leaf (Capillo virus)(e) Satsuma dwarf virus(f) Elsinoe australis (Sweet orange scab)(g) Sphaceloma fawcettii var. scabiosa (Tryon’s scab)(h) Radopholus citrophilus (Citrus burrowing nematode)a) Cadang cadang (viroid)b) Marasmiellus coco philus (Lethal boll rot)c) Rhadinaphelenchus cocophilus (palmarum (Red ring)Post-entry quarantine for a period of 6weeks in insect proof polyhouse/mesh house. Two inspections, onewhen the seedlings are 30 days oldand the second at 6 th week.Postentry quarantine for 45-60 days ininsect-proof screen house. Twoinspections, one at the time ofplanting and second at 45 days afterplanting.Post-entry quarantine for a period ofone year. First three months in insectproof glass/ poly/ mesh house andthereafter remaining period aftertransplantation in open isolated field.Four inspections at quarterlyintervals..Post-entry quarantine for a period ofone year. First three months in insectproof glass/ poly/ mesh house andthereafter remaining period aftertransplantation in open isolated field.Four inspections at quarterlyintervals.Postentry quarantine for one growthcycle. Three months in insect proofmesh house and thereafter remainingperiod after transplantation in openisolated field. First inspection at thetime of planting nuts in poly bagnursery, second inspection at the timeof transplanting in the isolated fieldand third inspection when seedlingsare two years old and final inspectionwhen the palms are at floweringstage.65

10. Coffea spp. (Coffee) andrelated species ofRubiaceae – seeds (freshbeans)/ rooted/un-rootedcuttings/plants(a) Mycena citricolor (American leaf spot)(b) Colletotrichum coffeanum var. virulens (Coffee berry disease)(c) Gibberella xylariodes (Tracheomycosis)(d) Hemeleia coffeicola (Powdery rust)(e) Pseudomonas syringae pv. garcae (Haloblight)(f) Phytomonas leptovasorum (Phloem necrosis)(g) Coffee ring spot virusPost entry quarantine for one yearperiod. First three months in glasshouse and thereafter remaining periodafter transplantation in open isolatedfield. Four inspections at quarterlyintervals.11. Dendranthemum spp(Chrysanthemum)-rooted/un-rootedcuttings/plants12. Dhalia spp (Dahlia)-tubers13. Dianthus spp(Carnation)-plants14. Diascorea spp (Yams)-tubers/plants(a) Rhodococcus fascians (Fasciation)(b) Aphelenchoides fragariae, A. ritzemabosi (Foliar nematodes)(c) Ditylenchus dipsaci (Stem and bulb nematode)(d) Puccinia horiana (White rust)(e) Didymella ligulicoa, syn. Ascochyta chrysanthemi (ray blight)(f) Chrysanthemum viruses viz. chlorotic mottle, stunt, veinchlorosis, virus B.(a) Dhalia mosaic virus (cauliflower mosaic virus)(b) Tomato spotted wilt virus(a) Burkholderia caryophilli (Bacterial wilt and stem cracking)(b) Erwinia chrysanthemi pv. dianthicola (Slow wilt)(c) Uromyces dianthi (Rust)(d) Sorosporium spaonariae (Smut)(e) Peronospora dianthi, P. dianthicola (Downy mildew)(f) Carnation viruses viz. latent, mottle virus(a) Rhizobacterium (Agrobacterium) tumefaciens (crown gall)(b) Yam mosaic virus/ green banding virusPostentry quarantine for 45-60 days ininsect proof polyhouse or mesh housewith top covered with polythene sheetfor rain protection. Two to threeinspections, with one at the time ofplanting and the second after 30 daysafter planting and third at 45 daysafter planting.Post-entry quarantine for one growthseason in isolated field. A minimumof two inspections, one at 30 daysafter planting or 4-leaf stage and thesecond inspection at flowering stage.Postentry quarantine for 45-60 days ininsect proof polyhouse or mesh housewith top covered with polythene sheetfor rain protection. Two to threeinspections, with one at the time ofplanting and the second after 30 daysafter planting and third at 45 daysafter planting. .Post-entry quarantine for one growthseason. First three months in insectproof glass/poly/mesh house andthereafter remaining period aftertransplantation in open isolated field.Four inspections at quarterlyintervals.66

15. Elaeis guineensis(oilpalm)-seeds/seedsprouts/plants16. Fragaria spp(strawberry)-rooted/unrootedcuttings/plants.17. Gerbera jamesonii(Gerbera)-plants18. Gladiolus spp (Gladioli)-corms.19. Hevea spp. (Rubber)-seed/budwwod(a) Fusarium oxysporum f.sp. elaeidis (Vascular wilt)(b) Cercospora elaedis (Freckle)(c) Rhadinaphelenchus cocophilus (Red ring)(d) Lethal bud rot or sudden wilt [Marchites sorpresiva(phytoplasmas)](e) Phytomonas staheli (Fatal wilt or hart rot)(f) Leaf mottle virus(g) Cadang cadang and related viroidsa) Phomopsis obscurens (Phomopsis blight)b) Phytophthora fragariae (Red stele)c) Phytophthora cactorum (Crown rot)d) Xanthomonas fragariae (Angular leaf spot)e) Xiphinema americanum (American dagger nematode)f) Gnomonia fragariae (Leaf blotch)g) Straw berry viruses viz., vein banding, crinkle leaf(rhabdovirus), mild yellow edge, latent ring spot (nepovirus),latent C.h) Aster yellows, straw berry green petal, phyllody & yellows(phytoplasmas).(a) Phytophthora cryptogea (tomato foot rot)(b) Tomato spotted wilt virus(c) Tobacco rattle virus(a) Urocystis gladiolicola (Smut)(b) Uromyces gladioli & U. transversalis (Rusts)(c Fusarium. oxysporum f.sp. gladioli (Corm rot)(d) Septoria gladioli (Hard rot)(e) Burkholderia marginalis (Scab & neck rot)(f) Burkholderia gladioli pv. gladioli (Base rot)(a) Microcyclus ulei (South American leaf blight)Post-entry quarantine for a period of10-12 months. First three months inprimary nursery and later insecondary nursery in poly bags inisolated area. 2-3 inspections, one atwhen the seedlings are 30-45 days oldand the second at the time oftransplanting in secondary nurseryand third at when seedlings attain 10months old.Post-entry quarantine for a period of 9months. First 30-45 days in insectproof poly/screen house andthereafter transplantation in openisolated field. A minimum of threeinspections, first when the seedlingsare 30 days old and second inspectionafter transplanting in isolated field (60days old) and final inspection, whenthe plants are at flowering/fruitingstage. .Postentry quarantine for 45-60 days ininsect proof polyhouse or mesh housewith top covered with polythene sheetfor rain protection. Two to threeinspections, with one at the time ofplanting and the second after 30 daysafter planting and third at 45 daysafter planting. .Post-entry quarantine for one growthseason in isolated field. A minimumof two inspections, one at 30 daysafter planting or 4-leaf stage and thesecond inspection at flowering stage.Post-entry quarantine for a period ofone year. First three months in glass67

20. Heliconia spprhizomes/plants.21. Hibiscus spp (Chinarose)-rooted/un-rootedcuttings/ plants22. Humulus spp (Hops)-rooted/un-rootedcuttings/plants23. Hyacinthus spp(Hyacinthus)-bulbs.house and thereafter remaining periodafter transplantation in open isolatedfield. Four inspections at quarterlyintervals.(a) Burkholderia solanacearum Race 2 (Moko wilt)Post-entry quarantine for a period of9-12 months. First three months ininsect proof glass/ poly/ mesh houseand thereafter remaining period aftertransplantation in open isolated field.A minimum of 3-4 inspections atquarterly intervals.(a) Hibiscus chlorotic ring spot virus Postentry quarantine for a period of 6months. First two months under glasshouse/polyhouse and there after inisolated field. A minimum of 3inspections, first inspection at thetime of planting, if rooted cuttings or30-45 days after planting in case ofun-rooted cuttings, second at the timeof transplanting in the isolated fieldand final inspection at 6 months.(a) Pseudoperonospora humuli (Downy mildew)(b) Heterodera humuli (Hops cyst nematode)(c) Hop latent (viroid)(a) Xanthomonas hyacinthi (Bacterial blight or yellow slime)(b) Hyacinth mosaic virus (Poty virus)(c) Ditylenchus dipsaci (Stem & bulb nematode)Postentry quarantine for a period of 6months. First two months under glasshouse/polyhouse and there after inisolated field. A minimum of 3inspections, first inspection at thetime of planting, if rooted cuttings or30-45 days after planting in case ofunrooted cuttings, second at the timeof transplanting in the isolated fieldand final inspection at 6 months.Postentry quarantine for one growthseason. A minimum of twoinspections, one at 30 days afterplanting or 4-leaf stage and thesecond inspection at flowering stage.68

24. Hydrangea spp(hydrangea)-rooted/unrootedcuttings25. Ipomaea spp (sweetpotato)-tubers/vinecuttings (rooted/unrooted)26. Iris spp. (Iris)-bulbs/rhizomes27. Juglans spp. (walnut)-rooted/un-rootedcuttings/plants28. Juniferus spp (Juniper)-plants(a) Puccinia glyceriae (hydrangea rust)(a) Elsinoe batatas (scab)(b) Moniliochaetes infuscans (Scurf)(c) Plenodomus destruens (foot rot)(d) Streptomyces ipomoeae (soil rot)(e) Pseudomonas batatae (Bacteria wilt)(f) Sweet potato viruses (Russet crack; feathery mottle; internalcork; chlorotic leaf spot; vein mosaic; mild mottle and yellowdwarf, vein clearing; chlorotic stunt; Sheffied’s virus A and Betc.)(g) Sweet potato witches’ broom (phytoplasmas)(a) Fusarium oxysporum f.sp. gladioli (Fusarial rot)(b) Ditylenchus dipsaci (Stem and bulb nematode)(c) Sclerotinia bulborum (Sclerotinia rot)(d) Iris virus (Potyvirus)(a) Xanthomonas juglandis (Bacterial blight)(b) Erwinia nigrifluens (Bark canker)(c) Euitypa armeniacae (Gummosis)(a) Gymnosporangium spp (Apple and pear rusts)-non Asiaticspecies29. Lillium spp (Lilly)-bulbs (a) Fusarium oxysporum f.sp. lilii (Fusarium wilt)(b) Colletotrichum lilii (Anthracnose)(c) Burkholderia gladioli pv. gladioli (Bacterial leaf spot)Postentry quarantine for 45-60 days ininsect proof polyhouse or mesh housewith top covered with polythene sheetfor rain protection. Two inspections,with one at the time of planting andthe second after 30 days afterplanting.Post-entry quarantine for one growthseason under insect proofglass/screen/poly house. A minimumof three inspections at monthlyintervals.Post-entry quarantine for one growthseason. A minimum of twoinspections, one at 30 days afterplanting or 4-leaf stage and thesecond inspection at flowering stage.Postentry quarantine for a period ofone year. First three months in insectproof glass/poly house and thereafterremaining period after transplantationin open isolated field. Fourinspections at quarterly intervals.Postentry quarantine for a period ofone year. First three months in glass/polyhouse and thereafter remainingperiod after transplantation in openisolated field. Four inspections atquarterly intervals.Post-entry quarantine for one growthseason. A minimum of twoinspections, one at 30 days after69

30. Limonium spp.(Limonium/ Statice)-plants31. Manihot esculenta(Cassava or tapioca)-tubers/cuttings (rooted/un-rooted)32. Morus spp (mulberry)-cuttings (rooted/unrooted)33. Musa spp (Abaca,banana, plantain)-rhizomes/suckers34. Nandina spp (Nandina)-plants(d) Lilly viruses (Lilly rosette, Lilly symptom less, tulip breakingand Lilly curl stripe)(a) Phytophthora cryptogea (tomato foot rot)(b) Limonium yellow vein virus (clover yellow vein virus)(c) tobacco rattle virus(d) Impatiens necrotic spot virus(e) tomato spotted wilt virus(a) Sphaceloma manihoticola (Super elongation)(b) (Xanthomonas campestris.pv. cassavae (Bacterial leaf spot)(c) Xanthomonas campestris pv. manihotis (Cassava bacterialblight) - American strain.(d) Cassava viruses (viz. common mosaic, brown streak, leaf veinmosaic, red mottle and yellow vein banding(e) Cassava witches’ broom (phytoplasma)(a) Pectobacterium rhapontici (rhubarb crown rot)(b) Rhizobium rhizogenes (hairy root)(c) Xylella fastidiosa (Pierce’s disease of grapevine)(a) Burkholderia solanacearum Race-2 (Moko wilt)(b) Mycosphaerella fijiensis var. difformis (Black leaf streak)(c) Cameroon marbling (Phytoplasmas)(d) Erwinia chrysanthemi pv. paradisiaca (Rhizome rot)(a) Clostero virus(b) Nandina mosaic virus(c) Nandina stem pitting capilovirusplanting or 4-leaf stage and thesecond inspection at flowering stage.Postentry quarantine for 45-60 days ininsect proof polyhouse or mesh housewith top covered with polythene sheetfor rain protection. Two inspections,with one at the time of planting andthe second after 30 days afterplanting.Post-entry quarantine for a period oftwo growth seasons inside insectproof screen house/poly house. Aminimum of two inspections in eachgrowth season, one after 30 days ofplanting and second 60-90 days afterplanting.Postentry quarantine for 45-60 days ininsect proof polyhouse or mesh housewith top covered with polythene sheetfor rain protection. Two to threeinspections, with one at the time ofplanting and the second after 30 daysafter planting and third at 45 daysafter planting.Post-entry quarantine for a period of9-12 months. First three months ininsect proof glass/ poly/ mesh houseand thereafter remaining period aftertransplantation in open isolated field.A minimum of 3-4 inspections atquarterly intervals.Postentry quarantine for 45-60 days ininsect proof polyhouse or mesh housewith top covered with polythene sheetfor rain protection. Two to threeinspections, with one at the time ofplanting and the second after 30 days70

35. Narcissus spp(Naarcissus)-bulbs36. Nicotiana spp (tobacco)-seed/plants(a) Fusarium oxysporum f. sp. narcissi (Basal rot)(b) Ditylenchus dipsaci (Stem and bulb nematode)(c) Botryotinia polyblastis (Narcissus fire)(d) Stagnospora curtissi (Leaf scorch)(e) Narcissus viruses(a) Peronospora tabacina (Blue mould)(b) Tobacco rattle virus37. Olea spp (Olive) (a) Arabis mosaic virus,(b) Cherry leaf roll virus(c) Olive latent ring spot virus(d) Olive partial paralysis virus(e) Olive sickle leaf virus(f) Strawberry latent ring spot virus38. Orchids (Aranda,Catteleya, Cymbidium,Dendrobium Lawliocatteleya,Mokara,Odontoglosum,Phalaenopsis, Vanda,Vanila etc)-plants39. Ornamental Palm species:(Arikuryoba,Borasus,Caryota,Carypha, Chamaeodorea,Chrysalidocorpus,Dictyosperma,Washingtonia, Roystonia,Hyophorbe, Pritchardia,Sabal, Syogrus,Trachycorpus, Vietchia,(a) Burkholderia gladioli pv. gladioli(Bacterial leaf spot)(b) Erwinia chrysanthemi (Soft rot)(c) Phyllostica capitalensis (Blossom blight)(d) Orchid viruses (Cymbidium mosaic, Vanilla necrosis,Odontoglosum ring spot, orchid fleck etc)(a) Acidovorax avenae sub sp. avenae (Bactrial blight)- ForCarypha spp only(b) Mosaic (Poty virus)- For Washingtonia spp only(c) Rhadinaphelenchus cocophilus (Red ring nematode)(d) Cadang cadang (viroid)after planting and third at 45 daysafter planting.Post-entry quarantine for one growthseason. A minimum of twoinspections, one at 30 days afterplanting or 4-leaf stage and thesecond inspection at flowering stage.Post-entry consignment for onegrowth season. A minimum of twoinspections, one at 30 days afterplanting or 4-leaf stage and thesecond inspection at flowering stage.Post-entry quarantine for a period of9-12 months. First three months ininsect proof glass/poly/mesh houseand thereafter remaining period aftertransplantation in open isolated field.A minimum of 3-4 inspections atquarterly intervals.Postentry quarantine for 45-60 days ininsect proof polyhouse or mesh housewith top covered with polythene sheetfor rain protection. Two to threeinspections, with one at the time ofplanting and the second after 30 daysafter planting and third at 45-60 daysafter planting. .Post-entry quarantine for a period of10-12 months. First three months inprimary nursery and later insecondary nursery in poly bags inisolated area. 2-3 inspections, one atwhen the seedlings are 30-45 days oldand the second at the time oftransplanting in secondary nurseryand third at when seedlings attain 10months old.71

Mascarena)-seeds/plants40. Pelargonium spp.(Pelargonium)-rooted/unrootedcuttings/plants.41. Phoenix dactylifera(datepalm)-suckers42. Pinus spp (Pine)-seeds/plants43. Pome Fruits: (Apple, Pear(Pyrus spp.) Quince(Cydonia spp.) andNectarine (Amygdalusspp))-cuttings (rooted/unrooted)/plants.44. Populus spp (Poplar)-rooted/un-rootedcuttings/plants(a) Xanthomonas campestris pv. pellargonii (Bacterial spot)(b) Pelargonium viruses viz. flower break virus, leaf curl virus,vein clearing virus and zonate spot virus.(a) Fusarium oysporum f.sp. albedinis (Bayood)(b) Palm lethal yellowing (Phytoplasmas)(c) Phymatotrichum omnivorum (Texas root rot)(a) Cronartium coleosporioides (Stalactiform blister rust)(b) C. comandrae (Comandra blister rust)(c) C. comptoniae (sweet fern blister rust)(d) C. fusiforme Southern fusiform rust)(e) Endocronartium harknessii (Western gall rust)(f) Mycosphaerella dearnesii, syn. Scirrhia acicola Brown spotneedle blight)(g) Fusarium moniliforme f.sp. subglutinans (seedling die-backand pitch canker).(h) Lophodermium spp(Needle cast)(i) Bursaphelenchus xylophilus (Pine wood nematode)(b) Erwinia amylovora (Fire blight)(c) Rhizobium (Agrobacterium) tumefaciens (Crown gall)(d) Rhizobium.. rhizogenes (Hairy root)(e) Gymnosporangium spp (Apple and pear rusts)-non Asiaticspecies(f) Apple scar skin, apple stem grooving viruses.(a) Hypoxylon mammatum (Hypoxylon canker)(b) Melampsora medusae (Poplar rust)(c) Mycosphaerella populorum, syn. Septoria musiva (Septoriacanker of poplar)Postentry quarantine for 45-60 days ininsect proof polyhouse or mesh housewith top covered with polythene sheetfor rain protection. Two to threeinspections, with one at the time ofplanting and the second after 30 daysafter planting and third at 45 daysafter planting. .Post-entry quarantine for a period ofone year.. First three months in insectproof glass/poly/mesh house andthereafter remaining period aftertransplantation in open isolated field.Four inspections at quarterlyintervals.Postentry quarantine for a period ofone year. First three months inglasshouse and thereafter remainingperiod after transplantation in openisolated field. Four inspections atquarterly intervals.Post-entry quarantine for a period of1-2 years. First three months in glass/poly house and thereafter remainingperiod after transplantation in openisolated field. Four inspections atquarterly intervals.Postentry quarantine for a period ofone year. Post-entry quarantine for aperiod of one year. First three monthsin insect proof poly house and72

45. Prunus spp (Almond,apricot, cherry, peach,plum, prune)-rooted/unrootedcuttings/plants(d) Euitypa armeniacae (Gummosis)(e) Poplar mosaic virus(a) Rhizobium (Agrobacterium) tumefaciens (Crown gall)(b) Rhizobium (Agrobacterium) rhizogenes (Hairy root)(c) Pseudomonas syringae pv. persicae syn. P. morsprunorum(Bacterial die back of peach)(d) Dibotryan morbosum (Black knot)(e) Euitypa armeniaceae (Gummosis)(f) Monilinia fructicola (Brown rot)-American strains(g) Monilinia. laxa (Blossom blight & fruit rot)(h) Venturia cerasi, V. carpophila (Scab)(i) Blumeriella jaapii (Cherry leaf spot)(j) Stone fruit viruses viz. Prunus virus S.46. Quercus spp (Oak)-plants (a) Ceratocystis fagacearum (Oak wilt)(b) Phytophthora ramorum (Ramorum wilt)47. Ribes spp (Goosberry &Currents)-rooted/unrootedcuttings/canes/plants48. Rosa spp (Rose)-rooted/un-rootedcuttings/budwood/ plants(a) Sphaerotheca mors-u-vae (American (gooseberry) mildew)(b) Microsphaeria grassulariae (European (gooseberry) mildew)(c) Pseudopeziza ribis (Leaf spot (Anthracnose))(d) Puccinia pringsheimiana (Cluster cup rust)(e) Plowrightia ribesia (Black pustule)(f) Botryosphaeria ribris (Cane blight)(g) Viruses viz., black current reversion, gooseberry vein banding,arabis mosaic, and strawberry latent ring spot.(a) Rhizobium (Agrobacterium) tumefaciens (Crown gall)(b) Rhizobium (Agrobacterium). rhizogenes (hairy root)(c) Coniothyrium wernsdorfiae (Brand canker)(d) Cryptosporella umbrina (Brown canker)(e) Peronospora sparsa (Downy mildew)(f) Phragmidium spp.( Rust)(g) Rose streak virus(h) Rose wilt virusthereafter remaining period aftertransplantation in open isolated field.Four inspections at quarterlyintervals.Post-entry quarantine for a period of 1year. First three months in insectproof glass/ poly/ mesh house andthereafter remaining period aftertransplantation in open isolated field.Four inspections at quarterlyintervals.Postentry quarantine for a period ofone year. First three months in insectproof poly/ mesh house and thereafterremaining period after transplantationin open isolated field. Fourinspections at quarterly intervals.Post-entry quarantine for a period of9-12 months. First three months ininsect proof glass/ poly/ mesh houseand thereafter remaining period aftertransplantation in open isolated field.A minimum of 3-4 inspections atquarterly intervals.Post-entry quarantine for a period of18 months for plants/rooted cuttingsin side insect proof polyhouse. Thebudded plants will be held for aperiod of 90 days in insect-proofpolyhouse. A minimum of 4inspections, the first one 30-45 daysafter planting and the subsequent73

49. Rubus spp (Raspberry)-rooted/un-rooted cuttings/plants(a) Rhizobium (Agrobacterium) tumefaciens (Crown gall)(b) Rhizobium (Agrobacterium). rhizogenes (hairy root)(c) Gymnoconia niten (rust),(d) Kuehneola uredinalis(rust),(e) Phragmedium bulbosum (rust),(f), Phragmedium rubi-idaeli (rust),(g) Phragmedium. Violacearum (rust),(h) Pucciniastrum americanum(rust)(i) Peronospora rubi (Downy mildew)(j) Viruses such as leaf mottle, leaf spot, bushy dwarf, leaf curl,raspberry (black) necrosis, vein chlorosis & yellow dwarf,arabis mosaic and straw berry shoestring.(a) Ditylenchus dipsaci (brown ring disease of hyacinth)(b) Arabis mosaic virus (hop bare-bine)inspections at 4-6 monthly intervals.In case of buddings, the firstinspection will be 30-45 days afterbudding, second at 60 days and thirdat 90 days after budding.Post-entry quarantine for a period of9-12 months. First three months ininsect proof glass/ poly/ mesh houseand thereafter remaining period aftertransplantation in open isolated field.A minimum of 3-4 inspections atquarterly intervals.50. Ranunculus spp.Post-entry quarantine for one growth(Ranunculus)-bulbsseason. A minimum of twoinspections, one at 30 days afterplanting or 4-leaf stage and thesecond inspection at flowering stage.51. Saccharum spp(a) Fiji virusPost-entry quarantine for a period of(sugarcane)-stem cuttings (b) Xanthomonas vasculorum (Gummosis)one year. First three months in insect(sets)(c) Sugarcane white leaf (phytoplasmas)proof glass/poly house and thereafter(d) Peronosclerospora sacchari (Sugarcane downy mildew) remaining period after transplantation(e) Pseudomonas rubrisubalbicans (Mottled stripe)in open isolated field. Fourinspections at quarterly intervals.52. Senecio spp.(a) Arabis mosaic virusPostentry quarantine for 45-60 days in(Senecio)-plants (b) Bidens mottle virusinsect proof polyhouse or mesh house(c) Beet western yellow viruswith top covered with polythene sheet(d) Chrysanthemum virus Bfor rain protection. Two to three(e) Tomato spotted wilt virusinspections, with one at the time ofplanting and the second after 30 daysafter planting and third at 45 daysafter planting. .53. Solanum tuberosum (a) Ditylenchus destructor (Potato tuber nematode) Post-entry quarantine for a period of74

(potato) and other tuberbearing Solanum speciestrueseed/tubers(b) Ditylenchus dipsaci (Stem and bulb nematode)(c) Globodera (Heterodera) rostochiensis & Globodera pallida(Potato cyst nematodes)(d) Phoma exigua var. foveata (Gangrene)(e) Synchytrium endobioticum (Potato wart)(f) Thecaphora (Angiosorus) solani (Potato smut)(g) Clavibacter michiganensis subsp. sepedonicus (Bacterial ringrot)(h) Potato viruses viz. Andean potato latent, Andean potatomottle, Arracacha B virus, Potato deforming mosaic, Potato T(capillo virus), Potato yellow dwarf, Potato yellow vein,Potato calico strain of Tobacco ring spot virus, Potato strainof Tobacco streak virus54. Syringa spp (Lilac)-plants (a) Arabis mosaic nepovirus(b) Cherry leaf roll virus (berteroa ringspot)(c) Elm mottle virus(d) Lilac ring mottle ilarvirus(e) Lilac mottle carlavirus(f) Lilac ring spot virus55. Theobroma cacao(cocoa)-seeds (freshbeans)/cuttings (rooted/un-rooted)/plants56. Triticum spp (wheat)-seeds(a) Swollen shoot virus and related strains(b) Crinipellis ( Marasmius) perniciosa (Witches’ broom)(c) Monilia (Moniliopthora) roreri (Watery pod rot)(d) Trachysphaera fructigena (Mealy pod)(a) Tilletia contraversa (Dwarf bunt)(b) Claviceps purpurea (Ergot)(c) Pseudomonas atrofaciens (Spike rot)two growth seasons inside insectproof screen house/poly house. Aminimum of two inspections in eachgrowth season, one after 30 days ofplanting and second 60-90 days afterplanting.Postentry quarantine for 45-60 days ininsect proof polyhouse or mesh housewith top covered with polythene sheetfor rain protection. Two to threeinspections, with one at the time ofplanting and the second after 30 daysafter planting and third at 45 daysafter planting. .Post-entry quarantine for a period ofone year. First three months in insectproof glass/ poly/ mesh house andthereafter remaining period aftertransplantation in open isolated field.Four inspections at quarterlyintervals.Postentry quarantine for one growthseason57. Tulipa spp. (Tulips)-bulbs (a) Ditylenchus dipsaci (Bulb and stem nematode)Post-entry quarantine for one growth(b) Curtobacterium flaccumfaciens pv. oortii (Yellow pustule & season. A minimum of twohellfire)(c) Tulipa viruses viz. band breaking, chlorotic blotch, virus x andother seed borne viruses.inspections, one at 30 days afterplanting or 4-leaf stage and thesecond inspection at flowering stage.58. Ulmus spp (Elm)- (a) Ceratocystis ulmi (Dutch elm disease) - American and Postentry quarantine for a period of75

seed/plants59. Vaccinium spp(blue/back berry)-rooted/un-rootedcuttings/plants60. Vitis spp (grapevine)-rooted/un-rooted cuttings/plants61. Zantedeschia spp (Callalilly)-corms62. Zingiber mioga(Ornamental zinger)-rhizomesEuropean strains(b) Elm mottle virus,(a) Pucciniastrum myrtili (Leaf rust)(b) Exobasidium vaccinii (Red leaf )(c) Pucciniastrum myrtili (Leaf rust)(d) Exobasidium vaccinii (Red leaf )(e) Synchytrium vaccinii (Red gall)(f) Pucciniastrum goeppertianum (Witches’broom)(g) Blue berry viruses viz., blue berry mosaic, shoe-string, red(necrotic) ring spot, leaf mottle, peach rosette and tomato ringspot(h) blueberry stunt, witches’broom and cranberry false blossom(phytoplasmas)(a) Phakopsora vitis (Rust)(b) Cryptosporella viticola syn. Phomopsis viticola (Dead arm)(c) Rhizobium (Agrobacterium) vitis (Cown gall)(d) Pantoea agglomerans (Gummosis)(e) Rhizobium (Agrobacterium) rhizogene (Hairy root)(f) Xylella f astidiosa (Pierce’s disease)(g) Xylophilus ampelinus (Bacterial necrosis)(h) Grapevine viruses: Luteovirus, Nepovirus, Closterovirus,Trichovirus, Potyvirus.(a) Xanthomonas campestris pv. zantedeschiae (Bacterial leafspot)(b) Zantadeschia mosaic virus(c) Tomato spotted wilt virus(d) Impatiens necrotic spot virus(a) Xanthomonas campestris pv. zingibericola (Leaf blight)one year. First three months in insectproof glass/ poly/ mesh house andthereafter remaining period aftertransplantation in open isolated field.Four inspections at quarterlyintervals.Post-entry quarantine for a period of9-12 months. First three months ininsect proof glass/ poly/ mesh houseand thereafter remaining period aftertransplantation in open isolated field.A minimum of 3-4 inspections atquarterly intervals..Post-entry quarantine for a period ofone year. Post-entry quarantine for aperiod of one year. First three monthsin insect proof glass/poly house andthereafter remaining period aftertransplantation in open isolated field.Four inspections at quarterlyintervals..Post-entry quarantine for one growthseason. A minimum of twoinspections, one at 30 days afterplanting or 4-leaf stage and thesecond inspection at flowering stage.Post-entry quarantine for one growthseason. A minimum of twoinspections, one at 30 days afterplanting or 4-leaf stage and thesecond inspection at flowering stage.76

SOP for Post Entry Quarantine Inspection - Plant quarantine India

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