This first annotated checklist of the lichens and associated fungi from Mingan Archipelago National Park Reserve (Québec, Canada) was compiled with the aim of enhancing the ecological knowledge from this regional biodiversity hotspot. I collected specimens in 2021 and 2022 and reviewed previous records based on vouchers deposited at the Louis-Marie Herbarium. A total of 257 species belonging to 96 genera are reported for the protected area, 3 of which represent the first published occurrence with an associated, digitized voucher for the province of Québec: Lecanactis abietina, Opegrapha vulgata, and Usnea diplotypus. This work highlights that Mingan Archipelago National Park Reserve has the potential to be recognized as an area of high lichen diversity in northeastern North America.