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Ultrastructure of Butyrivibrio fibrisolvens: a gram-positive bacterium.

Journal of Bacteriology, 01 Mar 1977, 129(3):1506-1512
https://doi.org/10.1128/jb.129.3.1506-1512.1977 PMID: 845122 PMCID: PMC235129

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Abstract 

The cells of bacteria of the genus Butyrivibrio are universally described as being gram negative, and they produce an unequivocal gram-negative reaction in the standard staining procedure. However, their cell walls contain derivatives of teichoic acid, which are characteristic of gram-positive cells. In this study, the cell walls of two representative strains of Butyrivibrio were of the gram-positive morphological type, as seen by electron microscopy, but they were very thin (12 to 18 nm). The thinness of these cell walls may account for the tendency of these cells to stain gram negatively in the standard staining procedure. Ruthenium red staining revealed an extracellular structure surrounding cells of Butyrivibio sp. (strain C3). This structure was composed of individual "knobs" that sometimes mediated cell-to-cell adhesion in the culture.

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J Bacteriol. 1977 Mar; 129(3): 1506–1512.
PMCID: PMC235129
PMID: 845122

Ultrastructure of Butyrivibrio fibrisolvens: a gram-positive bacterium.

K J Cheng and J W Costerton
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Abstract

The cells of bacteria of the genus Butyrivibrio are universally described as being gram negative, and they produce an unequivocal gram-negative reaction in the standard staining procedure. However, their cell walls contain derivatives of teichoic acid, which are characteristic of gram-positive cells. In this study, the cell walls of two representative strains of Butyrivibrio were of the gram-positive morphological type, as seen by electron microscopy, but they were very thin (12 to 18 nm). The thinness of these cell walls may account for the tendency of these cells to stain gram negatively in the standard staining procedure. Ruthenium red staining revealed an extracellular structure surrounding cells of Butyrivibio sp. (strain C3). This structure was composed of individual "knobs" that sometimes mediated cell-to-cell adhesion in the culture.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Akin DE. Ultrastructure of rumen bacterial attachment to forage cell walls. Appl Environ Microbiol. 1976 Apr;31(4):562–568. [Europe PMC free article] [Abstract] [Google Scholar]
  • BRYANT MP. Bacterial species of the rumen. Bacteriol Rev. 1959 Sep;23(3):125–153. [Europe PMC free article] [Abstract] [Google Scholar]
  • BRYANT MP, SMALL N. The anaerobic monotrichous butyric acid-producing curved rod-shaped bacteria of the rumen. J Bacteriol. 1956 Jul;72(1):16–21. [Europe PMC free article] [Abstract] [Google Scholar]
  • Cheng KJ, Costerton JW. Localization of alkaline phosphatase in three gram-negative rumen bacteria. J Bacteriol. 1973 Oct;116(1):424–440. [Europe PMC free article] [Abstract] [Google Scholar]
  • Cheng KJ, Costerton JW. Ultrastructure of cell envelopes of bacteria of the bovine rumen. Appl Microbiol. 1975 Jun;29(6):841–849. [Europe PMC free article] [Abstract] [Google Scholar]
  • Cheng KJ, Hironaka R, Jones GA, Nicas T, Costerton JW. Frothy feedlot bloat in cattle: production of extracellular polysaccharides and development of viscosity in cultures of Streptococcus bovis. Can J Microbiol. 1976 Apr;22(4):450–459. [Abstract] [Google Scholar]
  • Cheng KJ, Jones GA, Simpson FJ, Bryant MP. Isolation and identification of rumen bacteria capable of anaerobic rutin degradation. Can J Microbiol. 1969 Dec;15(12):1365–1371. [Abstract] [Google Scholar]
  • Costerton JW. The structure and function of the cell envelope of gram-negative bacteria. Rev Can Biol. 1970 Sep;29(3):299–316. [Abstract] [Google Scholar]
  • Costerton JW, Cheng KJ. The role of the bacterial cell envelope in antibiotic resistance. J Antimicrob Chemother. 1975 Dec;1(4):363–377. [Abstract] [Google Scholar]
  • Costerton JW, Damgaard HN, Cheng KJ. Cell envelope morphology of rumen bacteria. J Bacteriol. 1974 Jun;118(3):1132–1143. [Europe PMC free article] [Abstract] [Google Scholar]
  • Costerton JW, Ingram JM, Cheng KJ. Structure and function of the cell envelope of gram-negative bacteria. Bacteriol Rev. 1974 Mar;38(1):87–110. [Europe PMC free article] [Abstract] [Google Scholar]
  • Hewett MJ, Wicken AJ, Knox KW, Sharpe ME. Isolation of lipoteichoic acids from Butyrivibrio fibrisolvens. J Gen Microbiol. 1976 May;94(1):126–130. [Abstract] [Google Scholar]
  • Higgins ML, Shockman GD. Model for cell wall growth of Streptococcus faecalis. J Bacteriol. 1970 Feb;101(2):643–648. [Europe PMC free article] [Abstract] [Google Scholar]
  • HUNGATE RE. The anaerobic mesophilic cellulolytic bacteria. Bacteriol Rev. 1950 Mar;14(1):1–49. [Europe PMC free article] [Abstract] [Google Scholar]
  • Luft JH. Ruthenium red and violet. I. Chemistry, purification, methods of use for electron microscopy and mechanism of action. Anat Rec. 1971 Nov;171(3):347–368. [Abstract] [Google Scholar]
  • McCready RG, Costerton JW, Laishley EJ. Morphological modifications of cells of Clostridium pasteurianum caused by growth on sulfite. Can J Microbiol. 1976 Feb;22(2):269–275. [Abstract] [Google Scholar]
  • MURRAY RG, STEED P, ELSON HE. THE LOCATION OF THE MUCOPEPTIDE IN SECTIONS OF THE CELL WALL OF ESCHERICHIA COLI AND OTHER GRAM-NEGATIVE BACTERIA. Can J Microbiol. 1965 Jun;11:547–560. [Abstract] [Google Scholar]
  • Patterson H, Irvin R, Costerton JW, Cheng KJ. Ultrastructure and adhesion properties of Ruminococcus albus. J Bacteriol. 1975 Apr;122(1):278–287. [Europe PMC free article] [Abstract] [Google Scholar]
  • REYNOLDS ES. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. [Europe PMC free article] [Abstract] [Google Scholar]
  • SCOTT HW, DEHORITY BA. VITAMIN REQUIREMENTS OF SEVERAL CELLULOLYTIC RUMEN BACTERIA. J Bacteriol. 1965 May;89:1169–1175. [Europe PMC free article] [Abstract] [Google Scholar]
  • Sharpe ME, Brock JH, Phillips BA. Glycerol teichoic acid as an antigenic determinant in a Gram-negative bacterium Butyrivibrio fibrisolvens. J Gen Microbiol. 1975 Jun;88(2):355–363. [Europe PMC free article] [Abstract] [Google Scholar]
  • Wicken AJ, Knox KW. Lipoteichoic acids: a new class of bacterial antigen. Science. 1975 Mar 28;187(4182):1161–1167. [Abstract] [Google Scholar]
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