Large-spored Alternaria pathogens in section Porri disentangled.

Woudenberg JH; Truter M; Groenewald JZ; Crous PW

doi:10.1016/j.simyco.2014.07.003

Large-spored Alternaria pathogens in section Porri disentangled.

Affiliations

1. CBS-KNAW Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT Utrecht, Netherlands ; WUR, Laboratory of Phytopathology, Droevendaalsesteeg 1, 6708 PB Wageningen, Netherlands.
Authors
Woudenberg JH¹
(1 author)
2. ARC-Plant Protection Research Institute, P. Bag X134, Queenswood 0121, South Africa.
Authors
Truter M²
(1 author)
3. CBS-KNAW Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT Utrecht, Netherlands.
Authors
Groenewald JZ³
(1 author)
4. CBS-KNAW Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT Utrecht, Netherlands ; WUR, Laboratory of Phytopathology, Droevendaalsesteeg 1, 6708 PB Wageningen, Netherlands ; Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria 0002, South Africa.
Authors
Crous PW⁴
(1 author)

ORCIDs linked to this article

Studies in Mycology, 01 Sep 2014, 79:1-47
https://doi.org/10.1016/j.simyco.2014.07.003 PMID: 25492985 PMCID: PMC4255562

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Abstract

The omnipresent fungal genus Alternaria was recently divided into 24 sections based on molecular and morphological data. Alternaria sect. Porri is the largest section, containing almost all Alternaria species with medium to large conidia and long beaks, some of which are important plant pathogens (e.g. Alternaria porri, A. solani and A. tomatophila). We constructed a multi-gene phylogeny on parts of the ITS, GAPDH, RPB2, TEF1 and Alt a 1 gene regions, which, supplemented with morphological and cultural studies, forms the basis for species recognition in sect. Porri. Our data reveal 63 species, of which 10 are newly described in sect. Porri, and 27 species names are synonymised. The three known Alternaria pathogens causing early blight on tomato all cluster in one clade, and are synonymised under the older name, A. linariae. Alternaria protenta, a species formerly only known as pathogen on Helianthus annuus, is also reported to cause early blight of potato, together with A. solani and A. grandis. Two clades with isolates causing purple blotch of onion are confirmed as A. allii and A. porri, but the two species cannot adequately be distinguished based on the number of beaks and branches as suggested previously. This is also found among the pathogens of Passifloraceae, which are reduced from four to three species. In addition to the known pathogen of sweet potato, A. bataticola, three more species are delineated of which two are newly described. A new Alternaria section is also described, comprising two large-spored Alternaria species with concatenate conidia.

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Stud Mycol. 2014 Sep; 79: 1–47.

Published online 2014 Oct 16. https://doi.org/10.1016/j.simyco.2014.07.003

PMCID: PMC4255562

PMID: 25492985

Large-spored Alternaria pathogens in section Porri disentangled

J.H.C. Woudenberg,^1,^2, M. Truter,³ J.Z. Groenewald,¹ and P.W. Crous^1,^2,⁴

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Abstract

The omnipresent fungal genus Alternaria was recently divided into 24 sections based on molecular and morphological data. Alternaria sect. Porri is the largest section, containing almost all Alternaria species with medium to large conidia and long beaks, some of which are important plant pathogens (e.g. Alternaria porri, A. solani and A. tomatophila). We constructed a multi-gene phylogeny on parts of the ITS, GAPDH, RPB2, TEF1 and Alt a 1 gene regions, which, supplemented with morphological and cultural studies, forms the basis for species recognition in sect. Porri. Our data reveal 63 species, of which 10 are newly described in sect. Porri, and 27 species names are synonymised. The three known Alternaria pathogens causing early blight on tomato all cluster in one clade, and are synonymised under the older name, A. linariae. Alternaria protenta, a species formerly only known as pathogen on Helianthus annuus, is also reported to cause early blight of potato, together with A. solani and A. grandis. Two clades with isolates causing purple blotch of onion are confirmed as A. allii and A. porri, but the two species cannot adequately be distinguished based on the number of beaks and branches as suggested previously. This is also found among the pathogens of Passifloraceae, which are reduced from four to three species. In addition to the known pathogen of sweet potato, A. bataticola, three more species are delineated of which two are newly described. A new Alternaria section is also described, comprising two large-spored Alternaria species with concatenate conidia.

Key words: Alternaria, Early blight of potato, Early blight of tomato, Leaf and stem blight of sweet potato, Multi-gene phylogeny, Purple blotch of onion

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Introduction

Alternaria is an important fungal genus with a worldwide distribution. This hyphomycetous ascomycete with phaeodictyospores includes saprophytic, endophytic and pathogenic species, which can be plant pathogens, post-harvest pathogens or human pathogens (Thomma 2003). The genus Alternaria was recently divided into 24 sections (Woudenberg et al. 2013) based on molecular and morphological data, which followed the recent initiative to divide Alternaria into sections (Lawrence et al. 2013). Alternaria sect. Porri is the largest section, containing almost all Alternaria species with medium to large conidia and long beaks. Among them are some important plant pathogens, such as Alternaria bataticola, A. porri, A. solani and A. tomatophila. Alternaria bataticola causes leaf petiole and stem blight of sweet potato in tropical and sub-tropical regions. The disease is most severe in East and Central Africa, with yield losses of over 70 % reported (Osiru et al. 2007). Alternaria porri causes purple blotch of onion, a very destructive disease of onions worldwide. The disease causes a significant reduction in seed and bulb yield, with seed losses of up to 100 % (Abo-Elyousr et al. 2014). Alternaria solani is the causative agent of early blight of potato. This very common disease, which can be found in most potato-growing countries, can cause considerable defoliation. The disease typically reduces yields by ~20 %, but yield reductions of up to 80 % have been reported (Horsfield et al. 2010). Alternaria tomatophila is known for causing early blight of tomato, attacking the leaves, stems and fruit. This airborne pathogen has spread worldwide, mainly affecting field crops. When left untreated the damage can result in plant defoliation in excess of 60 % (Zitter & Drennan 2005).

The identification of these species has been problematic for many years, with every large-spored Alternaria found on Solanaceae commonly being identified as A. solani. This assumption changed with the treatment of Alternaria species on Solanaceae, in which Simmons (2000) distinguished 22 Alternaria and Nimbya species on solanaceous hosts on the basis of morphology. On potato, Simmons described the large-spored, long-beaked species A. grandis and A. solani, while on tomato he described A. tomatophila, A. cretica and A. subcylindrica. The distinction between potato and tomato pathogens was supported by subsequent molecular studies and chemotaxonomy (Andersen et al. 2008, Rodrigues et al. 2010, Brun et al. 2013, Gannibal et al. 2014).

The taxonomy of Alternaria species on Allium is also confused. Macrosporium porri was first described as pathogen of Allium (Cooke & Ellis 1879), followed by Alternaria allii (Nolla 1927). Both species were later synonymised (Angell 1929) and the name changed to Alternaria porri (Cifferi 1930). The name A. allii was resurrected by Simmons in his identification manual (2007) where he described five large-spored, long-beaked species from Allium, which he could distinguish based on morphology. Large-spored Alternaria from sweet potato were mostly identified as A. bataticola, even if the isolates from some studies (Osiru et al. 2008, Narayanin et al. 2010) showed morphological differences compared with the description of Simmons (2007).

In the present study we aim to use a molecular approach to delineate the medium- to large-spored Alternaria species with long beaks in sect. Porri. A multi-locus analysis based on five partial gene regions, the internal transcribed spacer regions 1 and 2 and intervening 5.8S nrDNA (ITS), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), RNA polymerase second largest subunit (RPB2), translation elongation factor 1-alpha (TEF1) and the Alternaria major allergen gene (Alt a 1), was performed. All available ex-type and representative isolates of medium to large-spored, long-beaked species described in Simmons (2007) were included in this study. The present multi-locus analysis supplemented with morphological and cultural data forms the basis for species recognition in sect. Porri.

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Materials and methods

Isolates

One hundred eighty-three Alternaria strains including 116 ex-type or representative strains present at the Centraalbureau voor Schimmelcultures (CBS), Utrecht, the Netherlands were included in this study (Table 1). With “representative isolate” we refer to the strains used to describe the species based on morphology in Simmons (2007). Freeze-dried strains were revived in 2 mL malt/peptone (50 % / 50 %) and subsequently transferred to oatmeal agar (OA, Crous et al. 2009). Strains stored in the liquid nitrogen collection of the CBS were transferred to OA directly from the −80 °C storage.

Table 1

Isolates used in this study and their GenBank accession numbers. Bold accession numbers were generated in other studies.

Name	Old name	Strain number¹	Status²	Host / Substrate	Locality	GenBank accesion numbers
						ITS	GAPDH	Alt a 1	TEF1	RPB2
Alternaria acalyphicola		CBS 541.94; E.G.S. 38.100; IMI 266969	T	Acalypha indica	Seychelles	KJ718097	KJ717952	KJ718617	KJ718446	KJ718271
Alternaria agerati		CBS 117221; E.G.S. 30.001; QM 9369	R	Ageratum houstonianum	USA, Illinois	KJ718098	KJ717953	KJ718618	KJ718447	KJ718272
Alternaria agripestis		CBS 577.94; E.G.S. 41.034	T	Euphorbia esula, stem lesion	Canada, Saskatchewan	KJ718099	JQ646356	KJ718619	KJ718448	KJ718273
Alternaria allii	Alternaria porri	CBS 107.28; E.G.S. 48.084	T	Allium cepa, leaf spot	Puerto Rico	KJ718100	KJ717954	KJ718620	KJ718449	KJ718274
	Alternaria porri	CBS 109.41; CBS 114.38		Allium cepa, seed	Denmark	KJ718101	KJ717955	KJ718621	KJ718450	KJ718275
	Alternaria porri	CBS 225.76		Allium porrum, leaf	Italy	KJ718102	KJ717956	KJ718622	KJ718451	KJ718276
		CBS 116701; E.G.S. 33.134	R	Allium cepa var. viviparum, floral bract	USA, Massachusetts	KJ718103	KJ717957	KJ718623	KJ718452	KJ718277
	Alternaria vanuatuensis	CBS 121345; E.G.S. 45.018	(T)	Allium cepa, leaf	Vanuatu	KJ718104	KJ717958	KJ718624	KJ718453	KJ718278
Alternaria alternariacida sp. nov.	Alternaria solani	CBS 105.51; ATCC 11078; IMI 46816; CECT 2997	T	Solanum lycopersicum, fruit	UK, England	KJ718105	KJ717959	KJ718625	KJ718454	KJ718279
Alternaria anagallidis		CBS 107.44		Anagallis arvensis, leaf spot	Denmark, Copenhagen	KJ718106	JQ646338	KJ718626	EU130544	KJ718280
		CBS 101004		Anagallis arvensis, leaf spot	New Zealand, Auckland	KJ718107	KJ717960	KJ718627	KJ718455	KJ718281
		CBS 117128; E.G.S. 42.074	R	Anagallis arvensis, leaf spot	New Zealand, Auckland	KJ718108	KJ717961	KJ718628	KJ718456	KJ718282
		CBS 117129; E.G.S. 50.091	R	Anagallis arvensis, leaf spot	New Zealand, Auckland	KJ718109	KJ717962	KJ718629	KJ718457	KJ718283
Alternaria anodae		PPRI 12376		Anoda cristata, leaf	South Africa, Gauteng	KJ718110	KJ717963	KJ718630	KJ718458	KJ718284
Alternaria aragakii		CBS 594.93; E.G.S. 29.016; QM 9046	T	Passiflora edulis	USA, Hawaii	KJ718111	KJ717964	KJ718631	KJ718459	KJ718285
Alternaria argyroxiphii		CBS 117222; E.G.S. 35.122	T	Argyroxiphium sp.	USA, Hawaii	KJ718112	JQ646350	KJ718632	KJ718460	KJ718286
		PPRI 11848		Ipomoea batatas, stem lesion	South Africa, Gauteng	KJ718113	KJ717965	KJ718633	KJ718461	KJ718287
		PPRI 11971		Ipomoea batatas, leaf and stem lesion	South Africa, Mpumalanga	KJ718114	KJ717966	KJ718634	KJ718462	KJ718288
Alternaria azadirachtae		CBS 116444; E.G.S. 46.195; BRIP 25386(ss1)	T	Azadirachta indica, leaf spot	Australia, Queensland	KJ718115	KJ717967	KJ718635	KJ718463	KJ718289
		CBS 116445; E.G.S. 46.196; BRIP 25386(ss2)	R	Azadirachta indica, leaf spot	Australia, Queensland	KJ718116	KJ717968	KJ718636	KJ718464	KJ718290
Alternaria bataticola		CBS 531.63; IFO 6187; MUCL 28916	T	Ipomoea batatas	Japan	KJ718117	JQ646349	JQ646433	KJ718465	KJ718291
		CBS 532.63		Ipomoea batatas	Japan, Tokyo	KJ718118	KJ717969	KJ718637	KJ718466	KJ718292
		CBS 117095; E.G.S. 42.157; IMI 350492; BRIP 19470a	R	Ipomoea batatas, leaf spot	Australia, Queensland	KJ718119	KJ717970	KJ718638	KJ718467	KJ718293
		CBS 117096; E.G.S. 42.158; BRIP 19470b	R	Ipomoea batatas, leaf spot	Australia, Queensland	KJ718120	KJ717971	KJ718639	KJ718468	KJ718294
		PPRI 10502		Ipomoea batatas, leaf and stem lesion	South Africa, Gauteng	KJ718121	KJ717972	KJ718640	KJ718469	KJ718295
		PPRI 11930		Ipomoea batatas, leaf lesion	South Africa, Kwazulu-Natal	KJ718122	KJ717973	KJ718641	KJ718470	KJ718296
		PPRI 11931		Ipomoea batatas, leaf lesion	South Africa, Kwazulu-Natal	KJ718123	KJ717974	KJ718642	KJ718471	KJ718297
		PPRI 11934		Ipomoea batatas, leaf lesion	South Africa, Gauteng	KJ718124	KJ717975	KJ718643	KJ718472	KJ718298
Alternaria blumeae	Alternaria brasiliensis	CBS 117215; E.G.S. 39.116	(R)	Phaseolus vulgaris, leaf spot	Brazil, Esperito Santo	KJ718125	KJ717976	KJ718644	KJ718473	KJ718299
		CBS 117364; E.G.S. 40.149; ATCC 201357	T	Blumea aurita	Thailand, Yala Province	KJ718126	AY562405	AY563291	KJ718474	KJ718300
Alternaria calendulae		CBS 224.76; ATCC 38903; DSM 63161; IMI 205077	T	Calendula officinalis, leaf spot	Germany	KJ718127	KJ717977	KJ718648	KJ718475	KJ718301
		CBS 101498		Calendula officinalis, leaf	New Zealand, Auckland	KJ718128	KJ717978	KJ718645	KJ718476	KJ718302
	Alternaria rosifolii	CBS 116439; E.G.S. 42.197	(T)	Rosa sp., leaf spot	New Zealand, Auckland	KJ718129	KJ717979	KJ718646	KJ718477	KJ718303
		CBS 116650; E.G.S. 30.142; QM 9561	R	Calendula officinalis, leaf spot	Japan, Tokyo	KJ718130	KJ717980	KJ718647	KJ718478	KJ718304
Alternaria carthami		CBS 635.80		Carthamus tinctorius, leaf	Italy, Perugia	KJ718131	KJ717981	KJ718649	KJ718479	KJ718305
	Alternaria heliophytonis	CBS 116440; E.G.S. 43.143; IMI 366164	(T)	Helianthus annuus, leaf	Canada, Saskatchewan	KJ718132	KJ717982	KJ718650	KJ718480	KJ718306
		CBS 117091; E.G.S. 31.037	R	Carthamus tinctorius, leaf spot	USA, Montana	KJ718133	KJ717983	KJ718651	KJ718481	KJ718307
Alternaria carthamicola	Alternaria carthami	CBS 117092; E.G.S. 37.057; IMI 276943	(R)T	Carthamus tinctorius	Iraq	KJ718134	KJ717984	KJ718652	KJ718482	KJ718308
Alternaria cassiae		CBS 478.81; E.G.S. 33.147	R	Senna obtusifolia, diseased seedling	USA, Mississippi	KJ718135	KJ717985	KJ718653	KJ718483	KJ718309
	Alternaria sauropodis	CBS 116119; E.G.S. 47.112; IMI 286317; IMI 392448	(T)	Sauropus androgynus	Malaysia, Sarawak	KJ718136	KJ717986	KJ718654	KJ718484	KJ718310
		CBS 117224; E.G.S. 40.121	R	Senna obtusifolia, leaf spot	Brazil, Federal District	KJ718137	KJ717987	KJ718655	KJ718485	KJ718311
	Alternaria hibiscinficiens	CBS 117369; E.G.S. 50.166	(T)	Hibiscus sabdariffa, leaf	Fiji	KJ718138	KJ717988	KJ718656	KJ718486	KJ718312
Alternaria catananches sp. nov.		CBS 137456; PD 013/05703936	T	Catananche caerulea	Netherlands	KJ718139	KJ717989	KJ718657	KJ718487	KJ718313
Alternaria centaureae		CBS 116446; E.G.S. 47.119	T	Centaurea solstitialis, leaf spot	USA, California	KJ718140	KJ717990	KJ718658	KJ718488	KJ718314
Alternaria cichorii		CBS 102.33; E.G.S. 07.017; QM 1760	T	Cichorium intybus, leaf spot	Cyprus	KJ718141	KJ717991	KJ718659	KJ718489	KJ718315
		CBS 117218; E.G.S. 52.046; IMI 225641	R	Cichorium endivia	Greece	KJ718142	KJ717992	KJ718660	KJ718490	KJ718316
Alternaria cirsinoxia		CBS 113261; E.G.S. 41.136	T	Cirsium arvense, stem lesion	Canada, Saskatchewan	KJ718143	KJ717993	KJ718661	KJ718491	KJ718317
Alternaria citrullicola sp. nov.	Alternaria cucumerina	CBS 103.32; VKM F-1881; Nattrass No. 190	T	Citrullus vulgaris, fruit	Cyprus	KJ718144	KJ717994	KJ718662	KJ718492	KJ718318
Alternaria conidiophora sp. nov.		CBS 137457	T	–	Netherlands	KJ718145	KJ717995	KJ718663	KJ718493	–
Alternaria crassa		CBS 103.18		Datura sp., leaf spot	USA, Wisconsin	KJ718146	KJ717996	KJ718664	KJ718494	KJ718319
		CBS 110.38	T	Datura stramonium, leaf spot	Cyprus	KJ718147	KJ717997	KJ718665	KJ718495	KJ718320
	Alternaria capsici	CBS 109160; E.G.S. 45.075; IMI 262408; IMI 381021	(T)	Capsicum annuum	Australia	KJ718148	AY562408	AY563298	KJ718496	KJ718321
		CBS 109162; E.G.S. 46.014		Nicandra physalodes	USA, Indiana	KJ718149	GQ180073	GQ180089	KJ718497	KJ718322
		CBS 116647; E.G.S. 46.013	R	Datura stramonium, leaf spot	USA, Indiana	KJ718150	KJ717998	KJ718666	KJ718498	KJ718323
		CBS 116648; E.G.S. 50.180	R	Datura stramonium, leaf spot	New Zealand, Auckland	KJ718151	KJ717999	KJ718667	KJ718499	KJ718324
		CBS 122590; E.G.S. 44.071	R	Datura stramonium, leaf spot	USA, Indiana	KJ718152	GQ180072	GQ180088	KJ718500	KJ718325
Alternaria cucumerina	Alternaria loofahae	CBS 116114; E.G.S. 35.123	(T)	Luffa acutangula	USA, Hawaii	KJ718153	KJ718000	KJ718668	KJ718501	KJ718326
		CBS 117225; E.G.S. 41.127	R	Cucumis melo, leaf spot	USA, Indiana	KJ718154	KJ718001	KJ718669	KJ718502	KJ718327
		CBS 117226; E.G.S. 44.197; BRIP 23060	R	Cucumis melo, leaf spot	Australia, Queensland	KJ718155	KJ718002	KJ718670	KJ718503	KJ718328
Alternaria cyamopsidis		CBS 364.67; E.G.S. 17.065; QM 8575	R	Cyamopsis tetragonoloba, leaf spot	USA, Maryland	KJ718156	KJ718003	KJ718671	KJ718504	KJ718329
		CBS 117219; E.G.S. 13.120; QM 8000	R	Cyamopsis tetragonoloba, leaf spot	USA, Georgia	KJ718157	KJ718004	KJ718672	KJ718505	KJ718330
Alternaria dauci		CBS 111.38	T	Daucus carota, seed	Italy	KJ718158	KJ718005	KJ718673	KJ718506	KJ718331
		CBS 106.48		Daucus carota, seed	–	KJ718159	KJ718006	KJ718674	KJ718507	KJ718332
		CBS 345.79; LEV 14814		Daucus carota, leaf spot	New Zealand, Ohakune	KJ718160	KJ718007	KJ718675	KJ718508	KJ718333
	Alternaria cichorii	CBS 477.83; CBS 721.79; PD 79/954		Cichorium intybus var. foliosum, leaf spot	Netherlands, Limburg	KJ718161	KJ718008	KJ718676	KJ718509	KJ718334
		CBS 101592		Daucus carota, seed	Netherlands	KJ718162	KJ718009	KJ718677	KJ718510	KJ718335
		CBS 117097; E.G.S. 46.006	R	Daucus carota, commercial seed	USA, California	KC584192	KC584111	KJ718678	KC584651	KC584392
		CBS 117098; E.G.S. 46.152	R	Daucus carota, leaf spot	New Zealand	KJ718163	KJ718010	HE796726	KJ718511	KJ718336
		CBS 117099; E.G.S. 47.131	R	Daucus carota, seed	USA, California	KJ718164	KJ718011	KJ718679	KJ718512	KJ718337
	Alternaria poonensis	CBS 117100; E.G.S. 47.138	(R)	Coriandrum sativum, seedling	Puerto Rico	KJ718165	JQ646348	KJ718680	KJ718513	KJ718338
Alternaria deserticola sp. nov.	Alternaria acalyphicola	CBS 110799	T	desert soil	Namibia	KJ718249	KJ718077	KJ718755	KJ718595	KJ718424
Alternaria dichondrae		CBS 199.74; E.G.S. 38.007	T	Dichondra repens, leaf spot	Italy	KJ718166	JQ646357	JQ646441	KJ718514	KJ718339
		CBS 200.74; E.G.S. 38.008	T	Dichondra repens, leaf spot	Italy	KJ718167	KJ718012	KJ718681	KJ718515	KJ718340
		CBS 346.79		Dichondra repens, leaf spot	New Zealand	KJ718168	KJ718013	KJ718682	KJ718516	KJ718341
		CBS 117127; E.G.S. 40.057	R	Dichondra sp., leaf	New Zealand, Auckland	KJ718169	KJ718014	KJ718683	KJ718517	KJ718342
Alternaria echinaceae		CBS 116117; E.G.S. 46.081	T	Echinacea sp., leaf lesion	New Zealand, Gisborne	KJ718170	KJ718015	KJ718684	KJ718518	KJ718343
		CBS 116118; E.G.S. 46.082	R	Echinacea sp., leaf lesion	New Zealand, Gisborne	KJ718171	KJ718016	KJ718685	KJ718519	KJ718344
Alternaria grandis		CBS 109158; E.G.S. 44.106	T	Solanum tuberosum, leaf spot	USA, Pennsylvania	KJ718239	JQ646341	JQ646425	EU130547	KJ718414
		CBS 116695; E.G.S. 44.108	R	Solanum tuberosum, leaf spot	USA, Pennsylvania	KJ718241	KJ718070	KJ718748	KJ718587	KJ718416
Alternaria euphorbiicola		CBS 198.86; E.G.S. 38.082		Euphorbia pulcherrima	USA, Florida	KJ718172	KJ718017	KJ718686	KJ718520	KJ718345
		CBS 119410; E.G.S. 41.029	R	Euphorbia pulcherrima	USA, Hawaii	KJ718173	KJ718018	–	KJ718521	KJ718346
		CBS 133874; E.G.S. 38.191		Euphorbia hyssopifolia	USA, Louisiana	KJ718174	KJ718019	KJ718687	KJ718522	KJ718347
Alternaria gypsophilae		CBS 107.41; E.G.S. 07.025; IMI 264349	T	Gypsophila elegans, seed	Netherlands	KC584199	KC584118	KJ718688	KC584660	KC584401
Alternaria ipomoeae sp. nov.	Alternaria cucumerina	CBS 219.79	T	Ipomoea batatas, stem and petiole	Ethiopia	KJ718175	KJ718020	KJ718689	KJ718523	KJ718348
		PPRI 8988		Ipomoea batatas, stem	South Africa, Gauteng	KJ718176	KJ718021	KJ718690	KJ718524	KJ718349
Alternaria jesenskae		CBS 133855; CCM 8361	T	Fumana procumbens, seed	Slovakia	KJ718177	KJ718022	KJ718691	KJ718525	KJ718350
Alternaria limicola		CBS 483.90; E.G.S. 39.070	T	Citrus aurantiifolia, leaf spot	Mexico, Colima	KJ718178	JQ646329	JQ646413	KJ718526	KJ718351
		CBS 117360; E.G.S. 43.009	R	Citrus sp.	Mexico, Jalisco	KJ718179	KJ718023	–	KJ718527	KJ718352
Alternaria linariae		CBS 105.41; E.G.S. 07.016	T	Linaria maroccana, seedling	Denmark	KJ718180	KJ718024	KJ718692	KJ718528	KJ718353
	Alternaria solani	CBS 108.53		–	–	KJ718181	KJ718025	KJ718693	KJ718529	KJ718354
	Alternaria solani	CBS 107.61		–	Belgium	KJ718182	KJ718026	KJ718694	KJ718530	KJ718355
	Alternaria tomatophila	CBS 109156; E.G.S. 42.156	(T)	Solanum lycopersicum, leaf spot	USA, Indiana	KJ718183	JQ646347	GQ180101	KJ718531	KJ718356
	Alternaria subcylindrica	CBS 109161; E.G.S. 45.113	(T)	Solanum lycopersicum var. cerasiforme, leaf spot	USA, Louisiana	KJ718184	JQ646345	JQ646429	KJ718532	KJ718357
	Alternaria cretica	CBS 109164; E.G.S. 46.188	(T)	Solanum lycopersicum, leaf spot	Greece, Crete	KJ718185	JQ646342	JQ646426	EU130545	KJ718358
	Alternaria cucumericola	CBS 116438; E.G.S. 41.057	(T)	Cucumis sativus, leaf spot	New Zealand	KJ718186	KJ718027	KJ718695	KJ718533	KJ718359
	Alternaria tabasco	CBS 116441; E.G.S. 45.108	(T)	Capsicum frutescens, leaf spot	USA, Louisiana	KJ718187	KJ718028	KJ718696	KJ718534	KJ718360
	Alternaria tomatophila	CBS 116704; E.G.S. 44.074	(R)	Solanum lycopersicum, leaf spot	USA, Indiana	KJ718188	KJ718029	KJ718697	KJ718535	KJ718361
		CPC 21620		Solanum lycopersicum, leaf spot	Thailand, Chiang Mai	KJ718189	KJ718030	KJ718698	KJ718536	KJ718362
Alternaria macrospora	Alternaria porri	CBS 106.29		Gossypium sp.	Nigeria	KJ718193	KJ718032	KJ718701	KJ718540	KJ718366
		CBS 117228; E.G.S. 50.190; ATCC 58172	T	Gossypium barbadense	USA, Arizona	KC584204	KC584124	KJ718702	KC584668	KC584410
Alternaria montanica		CBS 121343; E.G.S. 44.112; IMI 257563	T	Cirsium arvense	USA, Montana	KJ718194	KJ718033	KJ718703	KJ718541	KJ718367
Alternaria multirostrata		CBS 712.68; ATCC 18515; IMI 135454; MUCL 11722; QM 8820; VKM F-2997	T	Richardia scabra, floral bract	USA, Georgia	KJ718195	JQ646362	KJ718704	EU130546	KJ718368
		CBS 713.68; ATCC 18517; IMI 135455; MUCL 11715; QM 8821	R	Richardia scabra, floral bract	USA, Georgia	KJ718196	KJ718034	KJ718705	KJ718542	KJ718369
Alternaria neoipomoeae sp. nov.		PPRI 8990		Ipomoea batatas	South Africa, North West	KJ718197	KJ718035	KJ718706	KJ718543	KJ718370
		PPRI 11845	T	Ipomoea batatas, stem	South Africa, Gauteng	KJ718198	KJ718036	KJ718707	KJ718544	KJ718371
		PPRI 11847		Ipomoea batatas	South Africa, Mpumalanga	KJ718199	KJ718037	KJ718708	KJ718545	KJ718372
		PPRI 13903		Ipomoea batatas, leaf lesion	South Africa, Gauteng	KJ718200	KJ718038	KJ718709	KJ718546	KJ718373
Alternaria nitrimali		CBS 109163; E.G.S. 46.151	T	Solanum viarum, leaf spot	Puerto Rico	KJ718201	JQ646358	KJ718710	KJ718547	KJ718374
Alternaria novae-guineensis		CBS 116120; E.G.S. 47.198	T	Citrus sp., dry leaf	Papua New Guinea	KJ718202	KJ718039	KJ718711	KJ718548	KJ718375
		PPRI 12171		Galinsoga parviflora, leaf	South Africa, Gauteng	KJ718203	KJ718040	KJ718712	KJ718549	KJ718376
Alternaria obtecta		CBS 117367; E.G.S. 42.063	R	Euphorbia pulcherrima, leaf	USA, California	KJ718204	KJ718041	KJ718713	KJ718550	KJ718377
		CBS 134278; E.G.S. 42.064		Euphorbia pulcherrima	USA, California	KJ718205	KJ718042	KJ718714	KJ718551	KJ718378
Alternaria paralinicola sp. nov.	Alternaria linicola	CBS 116652; E.G.S. 47.157; DAOM 225747	(R)T	Linum usitatissimum, seed	Canada, Manitoba	KJ718206	KJ718043	KJ718715	KJ718552	KJ718379
Alternaria passiflorae		CBS 113.38		Passiflora edulis	Australia, South Queensland	KJ718207	JQ646353	JQ646437	KJ718553	KJ718380
	Alternaria solani	CBS 166.77		Capsicum frutescens, leaf	New Zealand, Waitakere	KJ718208	KJ718044	KJ718716	KJ718554	KJ718381
		CBS 629.93; E.G.S. 16.150; QM 8458	R	Passiflora edulis, fruit	New Zealand	KJ718209	KJ718045	KJ718717	KJ718555	KJ718382
	Alternaria hawaiiensis	CBS 630.93; E.G.S. 29.020; QM 9050	(T)	Passiflora edulis	USA, Hawaii	KJ718210	JQ646352	KJ718718	KJ718556	KJ718383
	Alternaria gaurae	CBS 116333; E.G.S. 50.121	(T)	Gaura lindheimeri, leaf	New Zealand, Auckland	KJ718211	KJ718046	KJ718719	KJ718557	KJ718384
		CBS 117102; E.G.S. 51.165	R	Passiflora ligularis, fruit spot	New Zealand, Auckland	KJ718212	KJ718047	KJ718720	KJ718558	KJ718385
		CBS 117103; E.G.S. 52.032	R	Passiflora caerulea, leaf spot	New Zealand, Auckland	KJ718213	KJ718048	KJ718721	KJ718559	KJ718386
Alternaria pipionipisi		CBS 116115; E.G.S. 40.096; IMI 340950	T	Cajanus cajan, seed	India	KJ718214	KJ718049	KJ718722	KJ718560	KJ718387
	Alternaria obtecta	CBS 117365; E.G.S. 42.048	(R)	Euphorbia pulcherrima, leaf	USA, California	KJ718215	KJ718050	KJ718723	KJ718561	KJ718388
	Alternaria obtecta	CBS 134265; E.G.S. 42.047		Euphorbia pulcherrima	USA, California	KJ718216	KJ718051	KJ718724	KJ718562	KJ718389
Alternaria porri	Alternaria allii	CBS 116649; E.G.S. 17.082; QM 8613	(R)	Allium cepa, leaf	USA, Nebraska	KJ718217	KJ718052	KJ718725	KJ718563	KJ718390
		CBS 116698; E.G.S. 48.147	R	Allium cepa, leaf spot	USA, New York	DQ323700	KC584132	KJ718726	KC584679	KC584421
		CBS 116699; E.G.S. 48.152	R,T	Allium cepa, leaf spot	USA, New York	KJ718218	KJ718053	KJ718727	KJ718564	KJ718391
Alternaria protenta	Alternaria solani	CBS 347.79; E.G.S. 44.091; LEV 14726; ATCC 38569		Solanum lycopersicum, fruit rot	New Zealand, Levin	KJ718219	KJ718054	KJ718728	KJ718565	KJ718392
	Alternaria hordeiseminis	CBS 116437; E.G.S. 32.076	(T)	Hordeum vulgare, seed	New Zealand	KJ718220	KJ718055	KJ718729	KJ718566	KJ718393
	Alternaria solani	CBS 116651; E.G.S. 45.020	(R)	Solanum tuberosum, tuber	USA, California	KC584217	KC584139	GQ180097	KC584688	KC584430
		CBS 116696; E.G.S. 45.023; IMI 372955	R	Helianthus annuus, leaf spot	Israel	KJ718221	JQ646335	JQ646419	KJ718567	KJ718394
		CBS 116697; E.G.S. 45.024; IMI 372957	R	Helianthus annuus, leaf spot	Israel	KJ718222	KJ718056	KJ718730	KJ718568	KJ718395
	Alternaria pulcherrimae	CBS 121342; E.G.S. 42.122; IMI 310506	(R)	Euphorbia pulcherrima	Australia, Queensland	KJ718223	KJ718057	KJ718731	KJ718569	KJ718396
	Alternaria solani	CBS 135189; E.G.S. 45.053	(R)	Solanum tuberosum	New Zealand, Hastings	KJ718224	GQ180082	GQ180098	KJ718570	KJ718397
Alternaria pseudorostrata		CBS 119411; E.G.S. 42.060	T	Euphorbia pulcherrima	USA, California	JN383483	AY562406	AY563295	KC584680	KC584422
Alternaria ranunculi		CBS 116330; E.G.S. 38.039; IMI 285697	T	Ranunculus asiaticus, seed	Israel	KJ718225	KJ718058	KJ718732	KJ718571	KJ718398
Alternaria ricini		CBS 215.31	T	Ricinus communis	Japan	KJ718226	KJ718059	KJ718733	KJ718572	KJ718399
		CBS 353.86		Ricinus communis	Italy, Sardinia	KJ718227	JQ646331	KJ718734	KJ718573	KJ718400
		CBS 117361; E.G.S. 06.181	R	Ricinus communis	USA, Virginia	KJ718228	KJ718060	KJ718735	KJ718574	KJ718401
Alternaria rostellata		CBS 117366; E.G.S. 42.061	T	Euphorbia pulcherrima, leaf	USA, California	KJ718229	JQ646332	KJ718736	KJ718575	KJ718402
Alternaria scorzonerae	Alternaria linicola	CBS 103.46; Elliot No. 45-190C		Linum usitatissimum	UK, Scotland	KJ718190	JQ646363	JQ646447	KJ718537	KJ718363
		CBS 478.83; E.G.S. 38.011	R,T	Scorzonera hispanica, leaf spot	Netherlands, Reusel	KJ718191	JQ646334	KJ718699	KJ718538	KJ718364
	Alternaria linicola	CBS 116703; E.G.S. 36.110; IMI 274549	(R)	Linum usitatissimum, seed	UK, Derbyshire	KJ718192	KJ718031	KJ718700	KJ718539	KJ718365
Alternaria sennae sp. nov.	Alternaria cassiae	CBS 477.81; E.G.S. 34.030; IMI 257253	(R)T	Senna corymbosa, leaf	India, Uttar Pradesh	KJ718230	JQ646344	JQ646428	EU130543	KJ718403
Alternaria sesami		CBS 240.73		Sesamum indicum	Egypt	KJ718231	JQ646343	KJ718737	KJ718576	KJ718404
		CBS 115264; CBS 117214; E.G.S. 13.027	R	Sesamum indicum, seedling	India	JF780939	KJ718061	KJ718738	KJ718577	KJ718405
Alternaria sidae		CBS 117730; E.G.S. 12.129	T	Sida fallax, leaf spot	Kiribati, Phoenix Islands	KJ718232	KJ718062	KJ718739	KJ718578	KJ718406
Alternaria silybi		CBS 134092; VKM F-4109	T	Silybum marianum, leaf	Russia, Vladivistok	KJ718233	KJ718063	KJ718740	KJ718579	KJ718407
		CBS 134093; VKM F-4117		Silybum marianum, leaf	Russia, Vladivistok	KJ718234	KJ718064	KJ718741	KJ718580	KJ718408
		CBS 134094; VKM F-4118		Silybum marianum, leaf	Russia, Vladivistok	KJ718235	KJ718065	KJ718742	KJ718581	KJ718409
Alternaria solani		CBS 106.21		–	–	KJ718236	KJ718066	KJ718743	KJ718582	KJ718410
		CBS 111.41		Solanum aviculare, leaf spot	–	KJ718237	KJ718067	KJ718744	KJ718583	KJ718411
	Alternaria danida	CBS 111.44; E.G.S. 07.029; QM 1772	(T)	Ageratum houstonianum, seed	Italy	Y17070	KJ718068	KJ718745	KJ718584	KJ718412
		CBS 109157; E.G.S. 44.098	R	Solanum tuberosum, leaf spot	USA, Washington	KJ718238	GQ180080	KJ718746	KJ718585	KJ718413
	Alternaria viciae-fabae	CBS 116442; E.G.S. 46.162; ICMP 10242	(T)	Vicia faba	New Zealand	KJ718240	KJ718069	KJ718747	KJ718586	KJ718415
Alternaria solani-nigri	Alternaria cyphomandrae	CBS 109155; E.G.S. 40.058	(T)	Cyphomandra betacea, fruit	New Zealand, New Plymouth	KJ718242	JQ646360	JQ646444	KJ718588	KJ718417
		CBS 113403; E.G.S. 51.106; CPC 10620	R	Solanum nigrum, leaf spot	New Zealand, Waikato	KJ718243	KJ718071	KJ718749	KJ718589	KJ718418
	Alternaria herbiculinae	CBS 116332; E.G.S. 49.180	(T)	Petroselinum crispum, stunted plant	New Zealand, Taranaki	KJ718244	KJ718072	KJ718750	KJ718590	KJ718419
	Alternaria glyceriae	CBS 116334; E.G.S. 51.107	(T)	Glyceria maxima, leaf spot	New Zealand, Waikato	KJ718245	KJ718073	KJ718751	KJ718591	KJ718420
	Alternaria beticola	CBS 116447; E.G.S. 47.196	(T)	Beta vulgaris, leaf spot	New Zealand, Canterbury	KJ718246	KJ718074	KJ718752	KJ718592	KJ718421
		CBS 117101; E.G.S. 51.032	R	Solanum nigrum, leaf spot	New Zealand, Waikato	KJ718247	KJ718075	KJ718753	KJ718593	KJ718422
	Alternaria ascaloniae	CBS 121347; E.G.S. 46.052	(T)	Allium ascalonicum, leaf spot	New Zealand, Hastings	KJ718248	KJ718076	KJ718754	KJ718594	KJ718423
Alternaria steviae		CBS 631.88; IFO 31212		Stevia rebaudiana, leaf spot	Japan, Kagawa	KJ718250	KJ718078	KJ718756	KJ718596	KJ718425
		CBS 632.88; IFO 31183		Stevia rebaudiana, leaf spot	Japan, Kagawa	KJ718251	JQ646339	KJ718757	KJ718597	KJ718426
		CBS 117362; E.G.S. 37.019; IFO 31182	T	Stevia rebaudiana, leaf spot	Japan, Kagawa	KJ718252	KJ718079	KJ718758	KJ718598	KJ718427
Alternaria tagetica		CBS 297.79; GST AM2		Tagetes sp., seed	UK	KJ718253	KJ718080	KJ718759	KJ718599	KJ718428
		CBS 298.79; GST AM3		Tagetes sp., seed	UK	KJ718254	KJ718081	KJ718760	KJ718600	KJ718429
		CBS 479.81; E.G.S. 33.081; GST 556	R	Tagetes erecta, seed	UK, England	KC584221	KC584143	KJ718761	KC584692	KC584434
		CBS 480.81; E.G.S. 33.184	R	Tagetes sp., seed	USA, South Carolina	KJ718255	KJ718082	KJ718762	KJ718601	KJ718430
		CBS 117217; E.G.S. 44.045	R	Tagetes sp., leaf spot	USA, Ohio	KJ718256	KJ718083	KJ718763	KJ718602	KJ718431
Alternaria thunbergiae		CBS 116331; E.G.S. 41.073; BRIP 14963	T	Thunbergia alata, leaf spot	Australia, Queensland	KJ718257	KJ718084	KJ718764	KJ718603	KJ718432
	Alternaria iranica	CBS 120986; E.G.S. 51.075	(T)	Allium cepa, leaf	Iran, Miandoab	KJ718258	KJ718085	KJ718765	KJ718604	KJ718433
		CBS 122597		Thunbergia alata	New Zealand, Auckland	KJ718259	KJ718086	KJ718766	KJ718605	KJ718434
Alternaria tillandsiae		CBS 116116; E.G.S. 43.074	T	Tillandsia usneoides	New Zealand	KJ718260	KJ718087	KJ718767	KJ718606	KJ718435
Alternaria tropica		CBS 631.93; E.G.S. 39.126	T	Passiflora edulis, fruit	USA, Florida	KJ718261	KJ718088	KJ718768	KJ718607	KJ718436
		CBS 117216; E.G.S. 39.125	R	Passiflora edulis, fruit	USA, Florida	KJ718262	KJ718089	KJ718769	KJ718608	KJ718437
Alternaria venezuelensis		CBS 116121; E.G.S. 48.065	T	Phaseolus vulgaris, leaf spot	Venezuela, Maracay	KJ718263	KJ718090	KJ718770	KJ718609	KJ718438
Alternaria zinniae		CBS 118.44		Callistephus chinensis, seed	Hungary	KJ718264	JQ646361	KJ718771	KJ718610	KJ718439
		CBS 107.48		Zinnia sp., leaf	Netherlands	KJ718265	KJ718091	KJ718772	KJ718611	KJ718440
		CBS 117.59		Zinnia elegans	Italy, Sardinia	KJ718266	KJ718092	KJ718773	KJ718612	KJ718441
		CBS 108.61		Zinnia elegans	–	KJ718267	KJ718093	KJ718774	KJ718613	KJ718442
		CBS 299.79		Zinnia sp., seed	UK	KJ718268	KJ718094	KJ718775	KJ718614	KJ718443
		CBS 300.79		Zinnia sp., seed	UK	KJ718269	KJ718095	KJ718776	KJ718615	KJ718444
		CBS 117223; E.G.S. 44.035	R	Zinnia elegans, leaf spot	New Zealand, Auckland	KJ718270	KJ718096	KJ718777	KJ718616	KJ718445

¹ATCC: American Type Culture Collection, Manassas, VA, USA; BRIP: Queensland Plant Pathology Herbarium, Queensland, Australia; CBS: Culture collection of the Centraalbureau voor Schimmelcultures, Fungal Biodiversity Centre, Utrecht, Netherlands; CCM: Czech Collection of Microorganisms, Brno, Czech Republic; CECT: Spanish Type Culture Collection, Valencia, Spain; CPC: Personal collection of P.W. Crous, Utrecht, Netherlands; DAOM: Canadian Collection of Fungal Cultures, Ottawa, Canada; DSM: German Collection of Microorganisms and Cell Cultures, Leibniz Institute, Braunschweig, Germany; E.G.S.: Personal collection of Dr. E.G. Simmons; Elliott: Personal collection of M.E. Elliott; GST: Personal collection of G.S. Taylor; ICMP: International Collection of Micro-organisms from Plants, Auckland, New Zealand; IFO: Institute for Fermentation Culture Collection, Osaka, Japan; IMI: Culture collection of CABI Europe UK Centre, Egham UK; LEV: Plant Health and Diagnostic Station, Levin, New Zealand; MUCL: (Agro)Industrial Fungi and Yeast Collection of the Belgian Co-ordinated Collections of Micro-organisms (BCCM), Louvain-la Neuve, Belgium; Nattrass: Personal collection of R.M. Nattrass; PD: Plant Protection Service, Wageningen, Netherlands; PPRI: ARC-Plant Protection Research Institute, Roodeplaat, South Africa; QM: Quarter Master Culture Collection, Amherst, MA, USA; VKM: All-Russian Collection of Microorganisms, Moscow, Russia.

²T: ex-type strain; R: representative strain; Letters between parentheses refer to synonymised species names; Bold letters are designated in this study.

PCR and sequencing

DNA extraction was performed using the UltraClean Microbial DNA isolation kit (Mobio laboratories, Carlsbad, CA, USA), according to the manufacturer's instructions. The ITS region was amplified with the primers V9G (de Hoog & Gerrits van den Ende 1998) and ITS4 (White et al. 1990), the GAPDH region with gpd1 and gpd2 (Berbee et al. 1999) the RPB2 region with RPB2–5F2 (Sung et al. 2007) and fRPB2–7cR (Liu et al. 1999), the TEF1 gene with the primers EF1-728F and EF1-986R (Carbone & Kohn 1999) or EF2 (O'Donnell et al. 1998) and the Alt a 1 region with the primers Alt-for and Alt-rev (Hong et al. 2005). The ITS, GAPDH, RPB2 and TEF1 PCRs were performed as described in Woudenberg et al. (2013). The reaction mixture for the Alt a 1 PCR consisted of 1 μL genomic DNA, 1 × NH₄ reaction buffer (Bioline, Luckenwalde, Germany), 3 mM MgCl₂, 20 μM of each dNTP, 0.2 μM of each primer and 0.25 U BIOTAQ DNA polymerase (Bioline). Conditions for PCR amplification consisted of an initial denaturation step of 5 min at 94 °C followed by 40 cycles of 30 s at 94 °C, 30 s at 55 °C and 60 s at 72 °C and a final elongation step of 7 min at 72 °C. The PCR products were sequenced in both directions using the PCR primers and the BigDye Terminator v. 3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA), and analysed with an ABI Prism 3730XL Sequencer (Applied Biosystems) according to the manufacturer's instructions. Consensus sequences were computed from forward and reverse sequences using the BioNumerics v. 4.61 software package (Applied Maths, St-Martens-Latem, Belgium). All newly generated sequences were deposited in GenBank (Table 1).

Phylogenetic analysis

Multiple sequence alignments were generated with MAFFT v. 7 (http://mafft.cbrc.jp/alignment/server/index.html), and adjusted by eye where necessary. Bayesian inference and Maximum Likelihood analyses were performed on both the individual sequence datasets as well as the concatenated datasets as described in Woudenberg et al. (2013), with the sample frequency set to 1000 instead of 100 in the Bayesian analysis. For the TEF1 partition an online tool (http://www.hiv.lanl.gov/content/sequence/findmodel/findmodel.html) suggested the K2P model with a gamma-rate variation as nucleotide substitution model, and for the remaining four partitions the TrN model with gamma-distributed rate variation. Sequences from the type species of the phylogenetically closest section, sect. Gypsophilae, A. gypsophilae (Woudenberg et al. 2013), were used as outgroup. The resulting trees were printed with TreeView v. 1.6.6 (Page 1996) and the alignments and trees deposited into TreeBASE (http://www.treebase.org).

Taxonomy

Cultures were incubated on potato carrot agar (PCA, Crous et al. 2009) and synthetic nutrient-poor agar (SNA, Nirenberg 1976) plates at moderate temperatures (~22 °C) under CoolWhite fluorescent light with an 8 h photoperiod. After 7 d the growth rates were measured and the colony characters noted. Colony colours were rated according to Rayner (1970). Morphological descriptions were made for isolates grown on SNA with a small piece of autoclaved filter paper placed onto the agar surface to enhance sporulation. When sporulation occurred, the sellotape technique was used for making slide preparations (Schubert et al. 2007) with Titan Ultra Clear Tape (Conglom Inc., Toronto, Canada) and Shear's medium as mounting fluid. The 95 % confidence intervals were derived from measurements of 30 structures, with extremes given in parentheses. Photographs of characteristic structures were made with a Nikon Eclipse 80i microscope equipped with a Nikon digital sight DS-Fi1 high definition colour camera, using differential interference contrast (DIC) illumination and the Nikon software NIS-Elements D v. 3.00. Adobe Bridge CS5.1 and Adobe Photoshop CS5 Extended, v. 12.1, were used for the final editing and photographic preparation. Colonies which did not sporulate after 7 d were checked for sporulation up to 3 wk; after this period they were noted as sterile. Nomenclatural data were deposited in MycoBank (Crous et al. 2004).

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Results

Phylogeny

Because the amplification/sequencing of the RPB2 region of CBS 137457 and the Alt a 1 region of CBS 119410 and CBS 117360 failed, these genes were included as missing data in the combined analysis of these isolates. The topologies of the trees obtained from the RAxML and Bayesian analyses were overall congruent, resulting in identical species-clades. The phylogenies of the single-gene trees were congruent with one exception, CBS 137456, which swapped between clusters with the different genes used, resulting in a somewhat distorted picture in the combined analysis. The aligned sequences of the ITS (538 characters), GAPDH (581 characters), RPB2 (772 characters), TEF1 (355 characters) and Alt a 1 (476 characters) gene regions of the 183 included Alternaria strains had a total length of 2 722 characters, with respectively 77, 111, 134, 141 and 131 unique site patterns. After discarding the burn-in phase trees, the Bayesian analysis resulted in 7 502 trees from which the 50 % majority rule consensus tree and posterior probabilities were calculated. The multi-gene phylogeny of section Porri (Fig. 1) divided the isolates in 62 species (clades) and one new Alternaria section. The species A. euphorbiicola and A. limicola, previously assigned to sect. Porri (Lawrence et al. 2013, Woudenberg et al. 2013), form a sister-clade to sect. Porri, here described as Alternaria sect. Euphorbiicola sect. nov. A Bayesian phylogeny based on the GAPDH, RPB2 and TEF1 sequences of representative isolates of the closely related sections in Alternaria (sequences obtained from Woudenberg et al. 2013) was constructed for comparison, with A. brassicicola CBS 118699 from sect. Brassisicola, as outgroup (Fig. 2).

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Fig. 1

Bayesian 50 % majority rule consensus tree based on the ITS, GAPDH, RPB2, TEF1 and Alt a 1 sequences of 183 Alternaria strains. The Bayesian posterior probabilities > 0.75 (PP) and RAxML bootstrap support values > 65 (ML) are given at the nodes (PP/ML). Thickened lines indicate a PP of 1.0 and ML of 100. Species names between parentheses represent synonymised species names. Ex-type strains are indicated with T and representative strains with R. Novel species names are printed in bold face. The tree was rooted to A. gypsophilae (CBS 107.41).

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Fig. 2

Bayesian 50 % majority rule consensus tree based on the GAPDH, RPB2 and TEF1 sequences of 41 Alternaria strains. The Bayesian posterior probabilities (PP) are given at the nodes. Thickened lines indicate a PP of 1.0. The tree was rooted to A. brassicola (CBS 118699).

Taxonomy

At the onset of this study, Alternaria sect. Porri contained 82 Alternaria species. After extensive phylogenetic analyses and morphological examination we now recognise 63 species in this section (Table 2), of which 10 are newly described. Twenty-seven species names are reduced to synonymy (Table 2). All isolates where taxonomic changes were found based on the multi-gene phylogeny were studied morphologically; photo plates of these species are included. Type details are only listed when typification is proposed.

Table 2

Current species within Alternaria sect. Porri and their host / substrate.

Species name	Synonymised names (this study)	Host / Substrate
Alternaria acalyphicola		Euphorbiaceae (Acalypha indica)
Alternaria agerati		Asteraceae (Ageratum houstonianum)
Alternaria agripestis		Euphorbiaceae (Euphorbia esula)
Alternaria allii	Alternaria vanuatuensis	Amaryllidaceae (Allium cepa, A. porrum)
Alternaria alternariacida		Solanaceae (Solanum lycopersicum)
Alternaria anagallidis		Primulaceae (Anagallis arvensis)
Alternaria anodae		Malvaceae (Anoda cristata)
Alternaria aragakii		Passifloraceae (Passiflora edulis)
Alternaria argyroxiphii		Asteraceae (Argyroxiphium sp.), Convolvulaceae (Ipomoea batatas)
Alternaria azadirachtae		Meliaceae (Azadirachta indica)
Alternaria bataticola		Convolvulaceae (Ipomoea batatas)
Alternaria blumeae	Alternaria brasilliensis	Asteraceae (Blumea aurita), Fabaceae (Phaseolus vulgaris)
Alternaria calendulae	Alternaria rosifolii	Asteraceae (Calendula officinalis), Rosaceae (Rosa sp.)
Alternaria carthami	Alternaria heliophytonis	Asteraceae (Carthamus tinctorius, Helianthus annuus)
Alternaria carthamicola		Asteraceae (Carthamus tinctorius)
Alternaria cassiae	Alternaria hibiscinficiens	Fabaceae (Senna obtusifolia), Malvacea (Hibiscus sabdariffa), Phyllanthaceae (Sauropus androgynus)
	Alternaria sauropodis
Alternaria catananches		Asteraceae (Catananche caerulea)
Alternaria centaureae		Asteraceae (Centaurea solstitialis)
Alternaria cichorii		Asteraceae (Cichorium endivia, C. intybus)
Alternaria cirsinoxia		Asteraceae (Cirsium arvense)
Alternaria citrullicola		Cucurbitaceae (Citrullus lanatus)
Alternaria conidiophora		Unknown
Alternaria crassa	Alternaria capsici	Solanaceae (Capsicum annuum, Datura stramonium, Nicandra physalodes)
Alternaria cucumerina	Alternaria loofahae	Cucurbitaceae (Cucumis melo, Luffa acutangula)
Alternaria cyamopsidis		Fabaceae (Cyamopsis tetragonoloba)
Alternaria dauci	Alternaria poonensis	Apiaceae (Daucus carota, Coriandrum sativum), Asteraceae (Cichorium intybus)
Alternaria deserticola		Soil
Alternaria dichondrae		Convolvulaceae (Dichondra sp., D. repens)
Alternaria echinaceae		Asteraceae (Echinacea sp.)
Alternaria grandis		Solanaceae (Solanum tuberosum)
Alternaria ipomoeae		Convolvulaceae (Ipomoea batatas)
Alternaria jesenskae		Cistaceae (Fumana procumbens)
Alternaria linariae	Alternaria cretica	Cucurbitaceae (Cucumis sativus), Scrophulariaceae (Linaria maroccana), Solanaceae (Capsicum frutescens, Solanum lycopersicum)
	Alternaria cucumericola
	Alternaria subcylindrica
	Alternaria tabasco
	Alternaria tomatophila
Alternaria macrospora		Malvaceae (Gossypium sp., G. barbadense)
Alternaria montanica		Asteraceae (Cirsium arvense)
Alternaria multirostrata		Rubiaceae (Richardia scabra)
Alternaria neoipomoeae		Convolvulaceae (Ipomoea batatas)
Alternaria nitrimali		Solanacaea (Solanum viarum)
Alternaria novae-guineensis		Asteraceae (Galinsoga parviflora), Rutaceae (Citrus sp.)
Alternaria obtecta		Euphorbiaceae (Euphorbia pulcherrima)
Alternaria paralinicola		Linaceae (Linum usitatissimum)
Alternaria passiflorae	Alternaria gaurae	Onagraceae (Gaura lindheimeri), Passifloraceae (Passiflora edulis, P. caerulea, P. ligularis), Solanaceae (Capsicum frutescens)
	Alternaria hawaiiensis
Alternaria pipionipisi		Euphorbiaceae (Euphorbia pulcherrima), Fabaceae (Cajanus cajan)
Alternaria porri		Amaryllidaceae (Allium cepa, A. porrum)
Alternaria protenta	Alternaria hordeiseminis	Asteraceae (Helianthus annuus), Euphorbiaceae (Euphorbia pulcherrima), Gramineae (Hordeum vulgare), Solanaceae (Solanum lycopersicum, S. tuberosum)
	Alternaria pulcherrimae
Alternaria pseudorostrata		Euphorbiaceae (Euphorbia pulcherrima)
Alternaria ranunculi		Ranunculaceae (Ranunculus asiaticus)
Alternaria ricini		Euphorbiaceae (Ricinus communis)
Alternaria rostellata		Euphorbiaceae (Euphorbia pulcherrima)
Alternaria scorzonerae	Alternaria linicola	Asteraceae (Sorzonerae hispanica), Linaceae (Linum usitatissimum)
Alternaria sennae		Fabaceae (Senna corymbosa)
Alternaria sesami		Pedaliaceae (Sesamum indica)
Alternara sidae		Malvaceae (Sida fallax)
Alternaria silybi		Asteraceae (Silybum marianum)
Alternaria solani	Alternaria danida	Asteraceae (Ageratum houstonianum), Fabaceae (Vicia faba), Solanaceae (Solanum aviculare, S. tuberosum)
	Alternaria viciae-fabae
Alternaria solani-nigri	Alternaria ascaloniae	Amaryllidaceae (Allium ascalonicum), Apiaceae (Petroselinum crispum), Chenopodiaceae (Beta vulgaris), Gramineae (Glyceria maxima), Solanaceae (Cyphomandra betacea, Solanum nigrum)
	Alternaria beticola
	Alternaria cyphomandrae
	Alternaria glyceriae
	Alternaria herbiculinae
Alternaria steviae		Asteraceae (Stevia rebaudiana)
Alternaria tagetica		Asteraceae (Tagetes sp., T. erecta)
Alternaria thunbergiae	Alternaria iranica	Acanthaceae (Thunbergia alata), Amaryllidaceae (Allium cepa)
Alternaria tillandsiae		Bromeliaceae (Tillandsia usneoides)
Alternaria tropica		Passifloraceae (Passiflora edulis)
Alternaria venezuelensis		Fabaceae (Phaseolus vulgaris)
Alternaria zinniae		Asteraceae (Callistephus chinensis, Zinnia sp., Z. elegans)

Section Porri D.P. Lawr., Gannibal, Peever & B.M. Pryor, Mycologia 105: 541. 2013

Type species: Alternaria porri (Ellis) Cif.

Section Porri is characterised by broadly ovoid, obclavate, ellipsoid, subcylindrical or obovoid, medium to large conidia, disto- and euseptate, solitary or in short chains, with a simple or branched, long to filamentous beak. Conidia contain multiple transverse and longitudinal septa and are slightly constricted near some transverse septa. Secondary conidiophores can be formed apically and/or laterally.

Species in sect. Porri

Alternaria acalyphicola E.G. Simmons, Mycotaxon 50: 260. 1994.

Material examined: Seychelles, from Acalypha indica (Euphorbiaceae), before Apr. 1982, C. Kingsland, culture ex-type of A. acalyphicola CBS 541.94 = E.G.S. 38.100 = IMI 266969.

Notes: Alternaria acalyphicola is closely related to A. ricini, with only 1 nt difference in three out of the five genes sequenced; RPB2, TEF1 and GAPDH. Based on this single isolate, the data is inconclusive to support the synonymy of these two species.

Alternaria agerati E.G. Simmons, Mycotaxon 65: 63. 1997.

= Alternaria agerati Sawada, Rep. Dept. Agric. Gov. Res. Inst. Formosa 86: 165. 1943. (nom. inval., Art. 36.1)

Material examined: USA, Illinois, Springfield, from Ageratum houstonianum (Asteraceae) in a commercial greenhouse, Nov. 1968, J.L. Forsberg, representative isolate of A. agerati CBS 117221 = E.G.S. 30.001 = QM 9369.

Alternaria agripestis E.G. Simmons & K. Mort., Mycotaxon 50: 255. 1994.

Material examined: Canada, Saskatchewan, Maxim, from infected stem of Euphorbia esula (Euphorbiaceae), 9 Jul. 1992, P. Harris, culture ex-type of A. agripestis CBS 577.94 = E.G.S. 41.034.

Alternaria allii Nolla, Phytopathology 17: 118. 1927. Fig. 3.

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Alternaria allii: conidia and conidiophores. A–C. CBS 107.28. D–E. CBS 109.41. F–H. CBS 225.76. I–L. CBS 121345. Scale bars = 10 μm.

= Alternaria vanuatuensis E.G. Simmons & C.F. Hill, CBS Biodiversity Ser. (Utrecht) 6: 260. 2007.

Materials examined: Denmark, from seed of Allium cepa (Amaryllidaceae), 1937, P. Neergaard, CBS 109.41 = CBS 114.38. Italy, from leaf of Allium porrum (Amaryllidaceae), 1974, H. Nirenberg, CBS 225.76. Puerto Rico, from leaf of Allium cepa, before 1928, J.A.B. Nolla, culture ex-type of A. allii CBS 107.28 = E.G.S. 48.084. USA, Massachusetts, Hadley, from floral bract of Allium cepa var. viviparum, 13 Jul. 1980, E.G. Simmons, representative of A. allii CBS 116701 = E.G.S. 33.134. Vanuatu, from leaves of Allium cepa, 1996, C.F. Hill, culture ex-type of A. vanuatuensis CBS 121345 = E.G.S 45.018.

Notes: Simmons (2007) designated the lectotype of A. allii as Nolla (1927), loc. cit., Pl. III, fig. 11–19, based on the absence of original Nolla specimens. In our study, however, we managed to uncover an original specimen, CBS 107.28, which was deposited in the CBS by J.A.B. Nolla in December 1927 as his “A. allii sp. nov.”, just after he published the new species description. We therefore recognise this isolate as the ex-type strain of A. allii. Isolate CBS 116701 did not sporulate after 3 wk of cultivation on SNA.

Alternaria alternariacida Woudenb. & Crous, sp. nov. MycoBank MB808990. Fig. 4.

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Alternaria alternariacida sp. nov. CBS 105.51: A–H. Conidia and conidiophores. Scale bars = 10 μm.

Etymology: Named after its ability to produce high amounts of alternaric acid.

Alternaria alternariacida differs from the ex-type isolate of its closest phylogenetic neighbour A. silybi (CBS 134092) based on alleles in three loci (positions derived from respective alignments of the separate loci deposited in TreeBASE): ITS position 386 (T), 497 (T), 498 (T); TEF1 position 3 (T), 18 (T); Alt a 1 position 205 (C), 336 (T), 339 (A), 350 (C), 404 (T), 408 (G).

Sporulation is atypical. Primary conidiophores solitary, simple, straight to slightly curved, septate, pale brown with a subhyaline tip, (52–)73–93(–155) × (4–)5–6(–7) μm, bearing a single, darkened, apical conidiogenous locus. Conidia solitary or in unbranched chains of 2(–3) conidia, conidium body pale olive-brown, smooth-walled, narrowly ovoid, solitary, non-catenulate, and secondary conidia (33–)44–49(–56) × (5–)7–8(–9) μm, with (3–)5–6(–8) transverse eusepta and no longitudinal septa; primary conidia in total (85–)99–111(–121) × (6–)7–8(–10) μm. The conidial body can be slightly constricted near the septa. The conidium body gradually tapers into mostly an aseptate, single, unbranched beak, but branched beaks do occur; apical and multiple lateral secondary conidiophores can also occur. Beaks (47−)129−257(−610) μm long, ca. 2 μm wide throughout their length. Sexual morph not observed.

Culture characteristics: After 7 d cultures on SNA flat, fimbriate, white; aerial mycelium sparse, white, colonies reaching 25−30 mm diam; cultures on PCA flat, entire, olivaceous in the centre with three olivaceous concentric circles and a buff to white margin; aerial mycelium fine, felty, white, colonies reaching 50 mm diam; reverse with four olivaceous concentric circles.

Material examined: UK, England, from fruit of Solanum lycopersicum (Solanaceae), 1946, P.W. Brian (holotype CBS H-21734, culture ex-type CBS 105.51 = ATCC 11078 = IMI 46816 = CECT 2997 = IBPG 14 = BRL408).

Note: The atypical sporulation of the single isolate of A. alternariacida, which is over 60 yr old, resulted in our decision to include sequence data in the species description.

Alternaria anagallidis A. Raabe, Hedwigia 78: 87. 1939.

Materials examined: Denmark, Copenhagen, from Anagallis arvensis (Primulaceae), before Mar. 1944, P. Neergaard, CBS 107.44. New Zealand, Auckland, Lynfield, from Anagallis arvensis, 4 May 1998, C.F. Hill, CBS 101004; Auckland, Lynfield, from Anagallis arvensis, 28 Jun. 1995, C.F. Hill, representative isolate of A. anagallidis CBS 117128 = E.G.S. 42.074; Auckland, from leaf spot of Anagallis arvensis, Jan. 2002, C.F. Hill, representative isolate of A. anagallidis CBS 117129 = E.G.S. 50.091.

Notes: Isolate CBS 107.44 differs on 6 nt positions in its RPB2 sequence from the other three A. anagallidis isolates included in this study. Because CBS 107.44 still clusters closest to the other A. anagallidis isolates, and since these isolates, from a single host species, form a distinct clade from all other Alternaria spp., we retained the name A. anagallidis for this isolate.

Alternaria anodae E.G. Simmons, Mycotaxon 88: 198. 2003.

Material examined: South Africa, Gauteng Province, Pretoria, ARC-Roodeplaat VOPI, from leaves of Anoda cristata (Malvaceae), 12 Jan. 2012, A. Thompson, PPRI 12376.

Alternaria aragakii E.G. Simmons, Mycotaxon 46: 181. 1993.

Material examined: USA, Hawaii, from Passiflora edulis (Passifloraceae), before Oct. 1968, M. Aragaki, culture ex-type of A. aragakii CBS 594.93 = E.G.S. 29.016 = QM 9046.

Alternaria argyroxiphii E.G. Simmons & Aragaki, Mycotaxon 65: 40. 1997.

Materials examined: South Africa, Gauteng Province, Pretoria, ARC-Roodeplaat VOPI, from stem lesion of Ipomoea batatas (Convolvulaceae), 20 Apr. 2005, A. Thompson, PPRI 11848; Mpumalanga Province, Marble Hall, from stem and leaf lesion of Ipomoea batatas, 22 Nov. 2011, A. Thompson, PPRI 11971. USA, Hawaii, Maui, Haleakala, from Argyroxiphium sp. (Asteraceae), 1969, M. Aragaki, culture ex-type of A. argyroxiphii CBS 117222 = E.G.S. 35.122.

Note: The host range of A. argyroxiphii is not restricted to Argyroxiphium, but has been broadened with the inclusion of two isolates from Ipomoea batatas (Convolvulaceae).

Alternaria azadirachtae E.G. Simmons & Alcorn, CBS Biodiversity Ser. (Utrecht) 6: 218. 2007.

Materials examined: Australia, Queensland, Tewantin, from Azadirachta indica (Meliaceae), 20 Jul. 1998, A. Bradley, culture ex-type of A. azadirachtae CBS 116444 = E.G.S. 46.195 = BRIP 25386 (ss1); additional strain from the same source, CBS 116445 = E.G.S. 46.196 = BRIP25386 (ss2).

Alternaria bataticola W. Yamam., Trans. Mycol. Soc. Japan 2(5): 89. 1960.

= Macrosporium bataticola Ikata, Agric. Hort. (Tokyo) 22: 241. 1947 (nom. inval., Art. 36.1).

Type: (Lectotype, designated in Simmons 2007) S. Ikata, Agric. & Hort. 22: 241. fig. 1. 1947.

Materials examined: Australia, Queensland, Walkamin, from leaf spot of Ipomoea batatas (Convolvulaceae), 5 Jul. 1991, collector unknown, representative isolate of A. bataticola CBS 117095 = E.G.S. 42.157 = IMI 350492 = BRIP 19470a; additional strain from the same source CBS 117096 = E.G.S. 42.158 = BRIP 19470b. Japan, Tokyo, from Ipomoea batatas, before Nov. 1963, collector unknown, CBS 532.63; from Ipomoea batatas, before Nov. 1963, collector unknown (epitype designated here CBS H-21743, MBT178114, culture ex-epitype CBS 531.63 = IFO 6187 = MUCL 28916). South Africa, Gauteng Province, Pretoria, ARC-Roodeplaat VOPI, from leaf and stem lesion of Ipomoea batatas, 16 Jun. 2010, M. Truter, PPRI 10502; Kwazulu-Natal Province, Empangeni, from leaf lesion of Ipomoea batatas, 4 Jul. 2011, A. Thompson, PPRI 11930; Kwazulu-Natal Province, Empangeni, from leaf lesion of Ipomoea batatas, 4 Jul. 2011, A. Thompson, PPRI 11931; Gauteng Province, Pretoria, ARC-Roodeplaat VOPI, from leaf lesion of Ipomoea batatas, 12 Jan. 2012, A. Thompson, PPRI 11934.

Alternaria blumeae E.G. Simmons & Sontirat, Mycotaxon 65: 81. 1997. Fig. 5.

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Alternaria blumeae: conidia and conidiophores. A–D. CBS 117364. E–H. CBS 117215. Scale bars = 10 μm.

= Alternaria brasiliensis F.M. Queiroz, M.F.S. Muniz & M. Menezes, Mycopathologia 150: 63. 2001.

Materials examined: Brazil, Espirito Santo, from leaf spot of Phaseolus vulgaris (Fabaceae), 1989, F.M. Queiroz, representative isolate of A. brasiliensis CBS 117215 = E.G.S. 39.116. Thailand, Yala Province, Amphoe Muang, from Blumea aurita (Asteraceae), 18 Jan. 1992, P. Sontirat, culture ex-type of A. blumeae CBS 117364 = E.G.S. 40.149 = ATCC 201357.

Notes: By synonymising A. brasiliensis with A. blumeae, the host range of this taxon has expanded to include Phaseolus vulgaris. The five sequenced genes are 100 % identical between the two examined specimens.

Alternaria calendulae Ondřej, Čas. Slez. Mus., Ser. A, Hist. Nat. 23: 150. 1974. Fig. 6.

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Alternaria calendulae: conidia and conidiophores. A–C. CBS 224.76. D–E. CBS 101498. F–H. CBS 116650. I–L. CBS 116439. Scale bars = 10 μm.

= Alternaria calendulae W. Yamam. 1939 (nom. nud.).

= Macrosporium calendulae Nelen, Bull. Centr. Bot. Gard. (Moscow) 35: 90. 1959 (nom. inval., Art. 36.1).

= Macrosporium calendulae Nelen, Bot. Mater. Otd. Sporov. Rast. Bot. Inst. Akad. Nauk S.S.S.R. 15: 144. 1962.

= Alternaria calendulae Nirenberg, Phytopathol. Z. 88: 108. 1977 (nom. illegit., Art. 53.1).

= Alternaria rosifolii E.G. Simmons & C.F. Hill, CBS Biodiversity Ser. (Utrecht) 6: 192. 2007.

Materials examined: Germany, former West-Germany, from leaf spot of Calendula officinalis (Asteraceae), 1974, H. Nirenberg, culture ex-type of A. calendulae Nirenberg CBS 224.76 = ATCC 38903 = IMI 205077 = DSM 63161. Japan, Tokyo, from leaf spot of Calendula officinalis, before 1964, representative isolate of A. calendulae CBS 116650 = E.G.S. 30.142 = QM 9561. New Zealand, Auckland, Kumeu, from leaf spot of Calendula officinalis, Oct. 1998, C.F. Hill, CBS 101498; Auckland, Mount Albert, from leaf of Rosa sp. (Rosaceae), before Feb. 1995, C.F. Hill, culture ex-type of A. rosifolii CBS 116439 = E.G.S. 42.197.

Note: By synonymising A. rosifolii with A. calendulae, the host range of this taxon has expanded to include Rosa.

Alternaria carthami S. Chowdhury, J. Indian Bot. Soc. 23: 65. 1944. Fig. 7.

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Alternaria carthami: conidia and conidiophores. A–D. CBS 117091. E–H. CBS 116440. Scale bars = 10 μm.

= Macrosporium anatolicum A. Săvul., Bull. Sect. Sci. Acad. Roumaine 26: 709. 1944.

= Alternaria heliophytonis E.G. Simmons, CBS Biodiversity Ser. (Utrecht) 6: 206. 2007.

Materials examined: Canada, Saskatchewan, Saskatoon, from leaf of Helianthus annuus (Asteraceae), 26 Aug. 1993, C. Jasalavich, culture ex-type of A. heliophytonis CBS 116440 = IMI 366164 = E.G.S. 43.143. Italy, Perugia, from leaf of Carthamus tinctorius (Asteraceae), before Nov. 1980, A. Zazzerini, CBS 635.80. USA, Montana, Sidney, from leaf spot of Carthamus tinctorius, 11 Jul. 1973, E.E. Burns, representative isolate of A. carthami CBS 117091 = E.G.S. 31.037.

Notes: Isolate CBS 635.80 did not sporulate after 3 wk cultivation on SNA. By synonymising A. heliophytonis with A. carthami, the host range of this taxon has expanded to include Helianthus annuus (Asteraceae).

Alternaria carthamicola Woudenb. & Crous, sp. nov. MycoBank MB808991. Fig. 8.

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Alternaria carthamicola sp. nov. CBS 117092: A–L. Conidia and conidiophores. Scale bars = 10 μm.

Etymology: Named after the host genus from which it was collected, Carthamus.

Primary conidiophores solitary or in small groups, simple, straight to slightly curved, septate, pale to dark brown with a subhyaline tip, (33–)55–71(–108) × 5–6(–7) μm, bearing a single, darkened, apical conidiogenous locus, but may produce geniculate conidiogenous extensions. Conidia solitary, rarely in chains of two conidia, conidium body pale olive-brown, mostly smooth-walled but sometimes ornamented at the base, ovoid, (39–)58–64(–82) × (13–)15–16(–17) μm; with (5–)6–7(–9) transverse and (1–)3(–4) longitudinal septa. Dark coloured eusepta can be formed during development; the conidial body is slightly constricted near the transverse septa. Conidia mostly have a septate, single to double filamentous beak, triple beaks are observed but not common, apical secondary conidiophores can be formed. Beaks (40–)158–186(–219) μm long, ca. 2 μm diam throughout their length and 4 μm at the base. Sexual morph not observed.

Culture characteristics: After 7 d cultures on SNA flat, rhizoid, white to opaque; aerial mycelium sparse, white, floccose, colonies reaching 55–60 mm diam; cultures on PCA flat, entire, olivaceous with three clear concentric circles; aerial mycelium fine, felty, olivaceous to olivaceous-grey, colonies reaching 65–70 mm diam; reverse shows four olivaceous concentric circles with an buff edge.

Material examined: Iraq, from Carthamus tinctorius (Asteraceae), 10 Apr. 1983, M.M. Elsahookie (holotype CBS H-21735, culture ex-type CBS 117092 = IMI 276943 = E.G.S. 37.057).

Notes: The new species A. carthamicola, originally identified as A. carthami, differs only on 9 nt positions in its RPB2 sequence from the other two A. carthami strains studied. Based on its RPB2 sequence it clusters with A. linicola.

Alternaria cassiae Jurair & A. Khan, Pakistan J. Sci. Industr. Res. 3: 72. 1960. Fig. 9.

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Alternaria cassiae: conidia and conidiophores. A–D. CBS 116119. E–H. CBS 117224. I–L. CBS 117369. Scale bars = 10 μm.

= Alternaria hibiscinficiens E.G. Simmons & C.F. Hill, Mycotaxon 88: 205. 2003.

= Alternaria sauropodis E.G. Simmons, CBS Biodiversity Ser. (Utrecht) 6: 340. 2007.

Materials examined: Brazil, Federal District, from leaf spot of Senna obtusifolia (Fabaceae), May 1990, G. Fiqueiredo, representative isolate of A. cassiae CBS 117224 = E.G.S. 40.121. Fiji, from leaf of Hibiscus sabdariffa (Malvaceae), Jun. 2002, C.F. Hill, culture ex-type of A. hibiscinficiens CBS 177369 = E.G.S. 50.166. Malaysia, Sarawak, Kuching, from Sauropus androgynus (Phyllanthaceae), 25 Apr. 1984, T.K. Kieh, culture ex-type of A. sauropodis CBS 116119 = IMI 286317 = IMI 392448 = E.G.S. 47.112. USA, Mississippi, Stoneville, from diseased seedling of Senna obtusifolia, before Oct. 1980, H.L. Walker, representative isolate of A. cassiae CBS 478.81 = E.G.S. 33.147.

Notes: Isolate CBS 478.81 did not sporulate after 3 wk incubation on SNA. By synonymising A. hibiscinficiens and A. sauropodis with A. cassiae, the host range of this taxon has expanded to include Sauropus androgynus (Euphorbiaceae) and Hibiscus sabdariffa (Malvaceae).

Alternaria catananches Woudenb. & Crous, sp. nov. MycoBank MB808992. Fig. 10.

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Alternaria catananches sp. nov. A–B. Disease symptoms on Catananche caerulea (photo's K.-H. Nugteren, Florensis B.V., Netherlands). C–L. CBS 137456: conidia and conidiophores. Scale bars = 10 μm.

Etymology: Named after its host genus from which it was isolated, Catananche.

Primary conidiophores solitary, simple, straight to curved, septate, pale brown, (31–)54–67(–94) × (5–)6(–7) μm, bearing a single, darkened, apical conidiogenous locus, but may produce geniculate conidiogenous extensions. Conidia solitary, conidium body pale olive-brown, ornamented in lower half of the conidium, narrowly ovoid, (26–)37–43(–57) × (7–)8–9(–11) μm, with (2–)4(–6) transverse septa and no longitudinal septa. Some darker coloured eusepta can be formed during development. The conidium body gradually tapers into a single, septate, unbranched beak; basal lateral secondary conidiophores can be formed. Beaks (77–)126–160(–260) μm long, ca. 2 μm diam throughout their length. Sexual morph not observed.

Culture characteristics: After 7 d cultures on SNA flat, entire/fimbriate, olivaceous around agar plug, white; aerial mycelium felty, white to olivaceous, colonies reaching 10–15 mm diam; cultures on PCA flat, erose, grey-olivaceous; aerial mycelium fine felty, olivaceous-grey; colonies reaching 25 mm diam; reverse identical.

Material examined: Netherlands, from Catananche caerulea (Asteraceae), 11 Dec. 2013, N. Troost-Riksen (holotype CBS H-21736, culture ex-type CBS 137456 = PD 013/05703936).

Notes: Alternaria catananches seems closely related to the A. cichorii isolates in the multi-gene phylogeny, but this is probably caused by long-branch attraction and incongruency between the different gene trees. Based on the ITS sequence it is identical to A. jesenskae, with RPB2 it is identical to A. cirsinoxia, with TEF1 it clusters with A. cichorii/A. cirsinoxia/A. carthami and with Alt a 1 it is identical to A. cichorii CBS 102.33, A. alternariacida and A. scorzonerae. Only its GAPDH sequences make it distinct from all other Alternaria species. Although the multi-gene tree does not provide strong support for separating it from the A. cichorii isolates, based on the individual gene sequences it is described here as a new Alternaria species.

Alternaria centaureae E.G. Simmons, CBS Biodiversity Ser. (Utrecht) 6: 236. 2007.

Specimen examined: USA, California, Sacramento, from Centaurea solstitialis (Asteraceae), Feb. 1999, D. Fogle, culture ex-type of A. centaureae CBS 116446 = E.G.S. 47.119.

Alternaria cichorii Nattrass, First List of Cyprus Fungi: 29. 1937.

≡ Alternaria porri f. sp. cichorii (Nattrass) T. Schmidt, Pflanzenschutzberichte 32: 181. 1965.

≡ Macrosporium cichorii (Nattrass) Gordenko, Mikol. Fitopatol. 9: 241. 1975.

Materials examined: Cyprus, from leaf spot of Cichorium intybus (Asteraceae), 1933, R.M. Nattrass (holotype IMI 1007, culture ex-type CBS 102.33 = E.G.S. 07.017 = QM 1760). Greece, Attica, from Cichorium endivia (Asteraceae), 24 Feb. 1978, S.D. Demetriades, representative isolate of A. cichorii CBS 117218 = E.G.S. 52.046 = IMI 225641.

Notes: Strain CBS 102.33 was deposited in Aug. 1933 in the CBS by R.M. Nattrass as A. cichorii sp. nov., with the remark that the description of the new species was in preparation. The holotype was subsequently deposited in IMI (IMI 1007) which consists of a dried herbarium specimen. In the present study we link CBS 102.33 as ex-type of A. cichorii to IMI 1007. The two isolates used in this study, CBS 102.33 and CBS 117218, differ only on 7 nt positions in their Alt a 1 sequence. Unfortunately CBS 102.33 is sterile, which does not provide additional information to support them as being two different species. Furthermore, the time difference of 45 yr between isolation of the two strains led to the decision to retain them as one species for now, pending fresh collections.

Alternaria cirsinoxia E.G. Simmons & K. Mort., Mycotaxon 65: 72. 1997.

Material examined: Canada, Saskatchewan, Watrous, from stem lesion and top dieback of Cirsium arvense (Asteraceae), 5 Aug. 1993, K. Mortensen, culture ex-type of A. cirsinoxia CBS 113261 = E.G.S. 41.136.

Alternaria citrullicola Woudenb. & Crous, sp. nov. MycoBank MB808993. Fig. 11.

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Fig. 11

Alternaria citrullicola sp. nov. CBS 103.32: A–H. Conidia and conidiophores. Scale bars = 10 μm.

Etymology: Named after the host genus from which it was collected, Citrullus.

Primary conidiophores solitary, simple, straight or sometimes curved, septate, pale brown with a subhyaline tip, (28–)35–52(–73) × (3–)4(–5) μm, bearing a single, darkened, apical conidiogenous locus. Conidia mostly solitary but chains of two conidia can occur, conidium body pale olive-brown, smooth-walled, narrowly ovoid, (28–)35–41(–56) × (6–)8(–10) μm; with (3–)5–6(–9) transverse distosepta and 0–1(–2) longitudinal septa. Conidia have a single, aseptate, unbranched filamentous beak; apical secondary conidiophores can be formed. Beaks (72–)178–232(–324) μm long, ca. 2 μm diam throughout their length. Sexual morph not observed.

Culture characteristics: After 7 d cultures on SNA flat, fimbriate, white to opaque with primrose sections near the edge; aerial mycelium sparse, fine felty, colonies reaching 45–50 mm diam; cultures on PCA flat, entire, olivaceous with three unclear concentric circles; aerial mycelium is sparse, pale olivaceous-grey, colonies reaching 50–55 mm diam; reverse shows olivaceous-buff to olivaceous rings.

Material examined: Cyprus, from fruit of Citrullus lanatus (Cucurbitaceae), before Jul. 1932, R.M. Nattrass (holotype CBS H-21742, culture ex-type CBS 103.32 = VKM F-1881).

Alternaria conidiophora Woudenb. & Crous, sp. nov. MycoBank MB808995. Fig. 12.

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Fig. 12

Alternaria conidiophora sp. nov. CBS 137457: A–H. Conidia and conidiophores. Scale bars = 10 μm.

Etymology: Named after its characteristically long, thick, conidiophores.

Primary conidiophores solitary, simple, mostly straight but sometimes curved, septate, dark brown with a subhyaline tip, (46–)89–105(–152) × (6–)7(–8) μm, bearing a single to multiple, darkened, long geniculate conidiogenous loci. Conidia solitary, conidium body olive-brown, smooth-walled, narrowly ovoid, (30–)45–52(–66) × (10–)12–13(–18) μm, with (2–)6–7(–9) transverse septa and (0–)1–2(–4) longitudinal septa. Darker coloured eusepta are formed during development. The conidial body is slightly constricted near the transverse septa. Conidia have a single, septate, unbranched, filamentous beak; basal, lateral secondary conidiophores can be formed. Beaks (49–)117–138(–186) μm long; ca. 2 μm diam throughout their length. Sexual morph not observed.

Culture characteristics: After 7 d cultures on SNA flat, fimbriate to rhizoid, white to opaque; aerial mycelium felty, white, colonies reaching 55–60 mm diam; cultures on PCA flat, entire, grey-olivaceous with two concentric circles; aerial mycelium wooly, pale olivaceous-grey, colonies reaching 55–60 mm diam; reverse identical.

Material examined: Netherlands, from unidentified host, Jul. 2011, U. Damm (holotype CBS H-21737, culture ex-type CBS 137457).

Alternaria crassa (Sacc.) Rands, Phytopathology 7: 337. 1917. Fig. 13.

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Fig. 13

Alternaria crassa: conidia and conidiophores. A–D. CBS 109162. E–H. CBS 116648. I–L. CBS 119160. Scale bars = 10 μm.

Basionym: Cercospora crassa Sacc., Michelia 1(no. 1): 88. 1877.

= Macrosporium solani Cooke, Grevillea 12: 32. 1883. (non M. solani Ellis & Martin, 1882)

= Cercospora daturae Peck, Rep. New York State Mus. Nat. Hist. 35: 140. 1884.

= Macrosporium cookei Sacc., Syll. Fungorum 4: 530. 1886. (nom. nov. in Saccardo for M. solani Cooke, 1883, non M. solani Ellis & Martin, 1882)

≡ Alternaria cookei (Sacc.) Bremer, Iʂmen, Karel, Özkan & M. Özkan, Istanbul Üniv. Fak. Mecm., B. 13: 42. 1948.

= Macrosporium daturae Fautrey, Rev. Mycol. (Toulouse) 16: 76. 1894.

≡ Alternaria daturae (Fautrey) Bubák & Ranoj., Fungi Imperf. Exsicc. Fasc. 14: 694. 1911.

= Alternaria capsici E.G. Simmons, Mycotaxon 75: 84. 2000.

Type: (Lectotype, designated in Simmons 2000) PAD, Cercospora crassa, Datura stramonium, S. [elva] ′76. 10.

Materials examined: Australia, from Capsicum annuum (Solanaceae), May 1981, D. Trimboli, culture ex-type of A. capsici CBS 109160 = IMI 262408 = IMI 381021 = E.G.S 45.075. Cyprus, Famagusta, from leaves of Datura stramonium (Solanaceae), Jan. 1936, R.M. Nattrass (epitype designated here CBS H-21744, MBT178115, culture ex-epitype CBS 110.38). New Zealand, Auckland, from leaf spot of Datura stramonium, 2002, C.F. Hill, representative isolate of A. crassa CBS 116448 = E.G.S. 50.180. USA, Indiana, Montgomery County, Nicandra physalodes (Solanaceae), 5 Sep. 1997, E.G. Simmons, CBS 109162 = E.G.S. 46.014; Indiana, from leaf spot of Datura stramonium, 5 Sep. 1997, E.G. Simmons, representative isolate of A. crassa CBS 116447 = E.G.S. 46.013; Indiana, Montgomery County, from leaf spot of Datura stramonium, 1 Aug. 1996, E.G. Simmons, representative isolate of A. crassa CBS 122590 = E.G.S. 44.071; Wisconsin, Madison, from leaf spot of Datura sp., before Apr. 1918, R.D. Rands, CBS 103.18.

Notes: Isolates CBS 110.38 and CBS 116647 did not sporulate after 3 wk incubation on SNA. By synonymising A. capsici with A. crassa, the host range of this taxon expanded to include Capsicum annuum, which also belongs to the Solanaceae.

Alternaria cucumerina (Ellis & Everh.) J.A. Elliott, Amer. J. Bot. 4: 472. 1917. Fig. 14.

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Fig. 14

Alternaria cucumerina: conidia and conidiophores. A–D. CBS 117225. E–H. CBS 117226. I–L. CBS 116114. Scale bars = 10 μm.

Basionym: Macrosporium cucumerinum Ellis & Everh., Proc. Acad. Nat. Sci. Philadelphia 47: 440. 1895.

= Alternaria loofahae E.G. Simmons & Aragaki, CBS Biodiversity Ser. (Utrecht) 6: 316. 2007.

Materials examined: Australia, Queensland, from leaf spot of Cucumis melo (Cucurbitaceae), Oct. 1996, R. O’Brien, representative isolate of A. cucumerina CBS 117226 = E.G.S. 44.197 = BRIP 23060. USA, Hawaii, Oahu, Waialua, from Luffa acutangula (Cucurbitaceae), 1971, M. Aragaki, culture ex-type of A. loofahae CBS 116114 = E.G.S. 35.123; Indiana, Knox County, from leaf spot of Cucumis melo, 1993, R.X. Latin, representative isolate of A. cucumerina CBS 117225 = E.G.S. 41.127.

Notes: The species clade for A. cucumerina does not have a clear support in the multi-gene phylogeny. CBS 117225 and CBS 117226 differ only on 2 nt in their RPB2 sequence, while the ex-type of A. loofahae (CBS 116114) differs on 1 nt from both A. cucumerina isolates in RPB2 and on 1 nt in Alt a 1. This internal variation in the two A. cucumerina isolates and the identical host family, Cucurbitaceae, with A. loofahae, supported the synonymy of A. loofahae. By synonymising A. loofahae with A. cucumerina, the host range of this taxon expanded to include Luffa acutangula.

Alternaria cyamopsidis Rangaswami & A.V. Rao, Indian Phytopathol. 10: 23. 1957.

≡ Alternaria cucumerina var. cyamopsidis (Rangaswami & A.V. Rao) E.G. Simmons, Mycopathol. Mycol. Appl. 29: 131. 1966.

Materials examined: USA, Georgia, from leaf spot of Cyamopsis tetragonoloba (Fabaceae), Jul. 1961, G. Sowell, representative isolate of A. cyamopsidis CBS 117219 = E.G.S. 13.120 = QM 8000; Maryland, Beltsville, from leaf spot of Cyamopsis tetragonoloba, 1964, R.G. Orellana, representative isolate of A. cyamopsidis CBS 364.67 = E.G.S. 17.065 = QM 8575.

Alternaria dauci (J.G. Kühn) J.W. Groves & Skolko, Canad. J. Res., Sect. C, Bot. Sci. 22: 222. 1944. Fig. 15.

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Fig. 15

Alternaria dauci. A. Disease symptoms on Daucus carota. B–L. Conidia and conidiophores. B–C. CBS 117097. D–F. CBS 117098. G–I. CBS 117099. J–L. CBS 117100. Scale bars = 10 μm.

Basionym: Sporidesmium exitiosum var. dauci J.G. Kühn, Hedwigia 1: 91. 1855.

≡ Polydesmus exitiosus var. dauci (J.G. Kühn) J.G. Kühn, Die Krankheiten der Kulturgewächse, ihre Ursachen und ihre Verhütung: 165. 1858.

≡ Macrosporium dauci (J.G. Kühn) Rostr., Tidsskr. Landoekon. ser. 5, 7: 385. 1888.

≡ Alternaria brassicae var. dauci (J.G. Kühn) Lindau, Rabenhorst‘s Kryptog.-Fl., Edn 2 (Leipzig) 1(9): 260. 1908.

≡ Alternaria porri f. sp. dauci (J.G. Kühn) Neerg, Danish species of Alternaria & Stemphylium: 252. 1945.

= Macrosporium carotae Ellis & Langl., J. Mycol. 6: 36. 1890.

≡ Alternaria carotae (Ellis & Langl.) J.A. Stev. & Wellman, J. Wash. Acad. Sci. 34: 263. 1944.

= Alternaria poonensis Ragunath, Mycopathol. Mycol. Appl. 21: 315. 1963.

Type: (Lectotype, designated in Simmons 1995) B, ms. spec. Sporidesmium exitiosum var. dauci Kühn, Leg. Gross Krausche p. Bunzlau, Jul. Kühn.

Materials examined: Italy, from seed of Daucus carota (Apiaceae), Sept. 1937, P. Neergaard (neotype designated here CBS H-21745, MBT178116, culture ex-neotype CBS 111.38). Netherlands, Limburg, Horst, from leaf spot in Cichorium intybus var. foliosum (Asteraceae), 1979, W.M. Loerakker, CBS 477.83 = CBS 721.79 = PD 79/954; from seed of Daucus carota, 1993, S&G Seeds, CBS 101592. New Zealand, from leaf spot of Daucus carota, Mar. 1998, C.F. Hill, representative isolate of A. dauci CBS 117098 = E.G.S. 46.152; Ohakune, from leaf spot of Daucus carota, before Jul. 1979, G.F. Laundon, CBS 345.79 = LEV 14814. Puerto Rico, from seedling of Coriandrum sativum (Apiaceae), 1999, W. Almodovar, representative isolate of A. poonensis CBS 117100 = E.G.S. 47.138. Unknown, from seed of Daucus carota, Jan. 1948, J.W. Groves, CBS 106.48. USA, California, from commercial seed of Daucus carota, Nov. 1994, B.M. Pryor, representative isolate of A. dauci CBS 117097 = E.G.S. 46.006; California, Kern County, from seed of Daucus carota, 1999, D. Fogle, representative isolate of A. dauci CBS 117099 = E.G.S. 47.131.

Notes: The indicated lectotype cannot be traced in B, and appears to be lost. We therefore designate CBS 111.38 as neotype. The isolates CBS 111.38, CBS 345.79 and CBS 101592 did not sporulate after 3 wk incubation on SNA.

Alternaria deserticola Woudenb. & Crous, sp. nov. MycoBank MB808996.

Etymology: Named after the substrate from which it was isolated, namely desert soil.

Culture sterile

Alternaria deserticola differs from the ex-type strain of its closest phylogenetic neighbour A. thunbergiae (CBS 116331) based on alleles in all five loci (positions derived from respective alignments of the separate loci deposited in TreeBASE): ITS position 165 (−), 373 (T), 381 (C), 383 (C), 488 (A); GAPDH position 484 (T); RPB2 position 76 (C), 88 (T), 91 (T), 139 (C), 211 (T), 316 (T), 490 (C), 496 (A), 646 (T), 670 (C), 671 (T), 673 (A), 760 (G); TEF1 position 37 (C), 49 (G), 197 (A), 223 (A), 274 (T), 277(–), 311(T); Alt a 1 position 10 (C), 209 (A), 210 (T), 220 (G), 322 (T), 452 (G).

Culture characteristics: After 7 d cultures on SNA flat, rhizoid, olivaceous-buff; aerial mycelium absent, colonies reaching 55 mm diam; cultures on PCA flat, entire, five grey-olivaceous concentric circles; aerial mycelium sparse, colonies reaching 75–80 mm diam; reverse shows five olivaceous-grey rings.

Material examined: Namibia, from desert soil, 2001, M. Christensen (holotype CBS H-21738, culture ex-type CBS 110799).

Note: The clear phylogenetic distinction of the sterile culture of A. deserticola from all other strains included in this study, resulted in our decision to describe this species based on sequence data only.

Alternaria dichondrae Gambogi, Vannacci & Triolo, Trans. Brit. Mycol. Soc. 65(2): 323. 1975.

Materials examined: Italy, Pisa, from leaf spot of Dichondra repens (Convolvulaceae), Mar. 1974, P. Gambogi, ex-isotype of A. dichondrae CBS 199.74 = E.G.S. 38.007; Pisa, from leaf spot of Dichondra repens, Mar. 1974, P. Gambogi, living lectotype of A. dichondrae CBS 200.74 = E.G.S. 38.008. New Zealand, from leaf spot of Dichondra repens, before 1979, G.F. Laundon, CBS 346.79; Auckland, Lynfield, from leaf of Dichondra sp., Apr. 1991, C.F. Hill, representative isolate of A. dichondrae CBS 117127 = E.G.S. 40.057.

Note: Simmons (2007) designated a lectotype with ex-lectotype strain (CBS 200.74), as he found the ex-isotype strain (CBS 199.74) to be sterile.

Alternaria echinaceae E.G. Simmons & C.F. Hill, CBS Biodiversity Ser. (Utrecht) 6: 318. 2007.

Materials examined: New Zealand, Gisborne, Makaraka, from leaf of Echinacea sp. (Asteraceae), Jan. 1998, C.F. Hill, culture ex-type of A. echinaceae CBS 116117 = E.G.S. 46.081; Gisborne, Makaraka, from leaf of Echinacea sp., Jan. 1998, C.F. Hill, representative isolate of A. echinaceae CBS 116118 = E.G.S. 46.082.

Alternaria grandis E.G. Simmons, Mycotaxon 75: 96. 2000. Fig. 16.

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Fig. 16

Alternaria grandis: conidia and conidiophores. A–D. CBS 109158. E–H. CBS 116695. Scale bars = 10 μm.

Materials examined: USA, Pennsylvania, Centre County, from leaf lesion of Solanum tuberosum (Solanaceae), Sep. 1966, B.J. Christ, culture ex-type of A. grandis CBS 109158 = E.G.S. 44.106; Pennsylvania, Clarion County, from leaf spot of Solanum tuberosum, Sep. 1966, B.J. Christ, representative isolate of A. grandis CBS 116695 = E.G.S 44.108.

Notes: Although A. grandis differs by only 1 nt in its GAPDH sequence from A. solani, we retain it as a distinct species. Conidia of A. grandis are substantially larger than those of A. solani, and a recently published study could separate A. solani (CBS 109157) and A. grandis (CBS 109158) based on partial calmodulin gene sequence data (Gannibal et al. 2014).

Alternaria ipomoeae M. Truter, Woudenb. & Crous, sp. nov. MycoBank MB808997. Fig. 17.

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Fig. 17

Alternaria ipomoeae sp. nov. CBS 219.79: A–L. Conidia and conidiophores. Scale bars = 10 μm.

Etymology: Named after the host genus on which it occurs, Ipomoea.

Primary conidiophores simple to branched, straight to slightly curved, septate, pale brown, (10–)51–73(–145) × (4–)5 μm, bearing a single to multiple, darkened, geniculate conidiogenous loci. Conidia mostly solitary but chains of two conidia can occur, conidium body olive-brown, smooth-walled with ornamented base, long ellipsoid to obclavate, (53–)60–65(–76) × (9–)12(–15) μm, with (6–)8–9(–12) transverse septa and (0–)2(–3) longitudinal septa. Up to four dark coloured eusepta can be formed during development; the conidial body is constricted near these eusepta. Conidia have a septate, single to double, filamentous beak; apical and lateral secondary conidiophores can be formed. Beaks (47–)136–162(–221) μm long, single beaks generally longer than multiple beaks, ca. 2 μm diam throughout their length, and approx. 3 μm diam at the base. Sexual morph not observed.

Culture characteristics: After 7 d cultures on SNA are flat, fimbriate, white; aerial mycelium sparse, felty, white, colonies reaching 50 mm diam; cultures on PCA flat, entire, grey-olivaceous with some darker sections; aerial mycelium fine felty, pale olivaceous-grey, colonies reaching 65–70 mm diam; reverse identical.

Materials examined: Ethiopia, from black lesions of Ipomoea batatas (Convolvulaceae), Jun. 1978, A.H.C. van Bruggen (holotype CBS H-21739, culture ex-type CBS 219.79). South Africa, Gauteng Province, Pretoria, ARC-Roodeplaat VOPI, from stem lesions of Ipomoea batatas, 16 Nov. 2006, C.D. Narayanin (paratype PREM 60979, culture ex-paratype PPRI 8988).

Alternaria jesenskae Labuda, P. Eliáš & Sterfl., Microbiol. Res. 163: 209. 2008.

Material examined: Slovakia, district of the village Muzla, Podunajská nizina lowland, from seeds of Fumana procumbens (Cistaceae), Aug. 1999, P. Eliáš jr., culture ex-type of A. jesenskae CBS 133855 = CCM 8361.

Alternaria linariae (Neerg.) E.G. Simmons, CBS Biodiversity Ser. (Utrecht) 6: 677. 2007. Fig. 18.

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Fig. 18

Alternaria linariae. A. Disease symptoms on Solanum lycopersicum. B–P. Conidia and conidiophores. B–C. CBS 105.41. D–F. CBS 109161. G–H. CBS 107.61. I–J. CBS 109156. K–L. CBS 109164. M–N. CBS 116438. O–P. CBS 116441. Scale bars = 10 μm.

Basionym: Alternaria anagallidis var. linariae Neerg., Danish species of Alternaria & Stemphylium: 297. 1945.

= Alternaria cretica E.G. Simmons & Vakal., Mycotaxon 75: 64. 2000.

= Alternaria subcylindrica E.G. Simmons & R.G. Roberts, Mycotaxon 75: 62. 2000.

= Alternaria tomatophila E.G. Simmons, Mycotaxon 75: 53. 2000.

= Alternaria cucumericola E.G. Simmons & C.F. Hill, CBS Biodiversity Ser. (Utrecht) 6: 210. 2007.

= Alternaria tabasco E.G. Simmons & R.G. Roberts, CBS Biodiversity Ser. (Utrecht) 6: 158. 2007.

Materials examined: Belgium, host unknown, before Mar. 1961, R. Sys, CBS 107.61. Denmark, from seedling of Linaria maroccana (Scrophulariaceae), 13 Nov. 1940, P. Neergaard, culture ex-type of A. linariae CBS 105.41 = E.G.S. 07.016. Greece, Crete, Heraklio, from leaf spot of Solanum lycopersicum (Solanaceae), 1997, D.J. Vakalounakis, culture ex-type of A. cretica, CBS 109164 = E.G.S. 46.188. New Zealand, Northland, Kerikeri, from leaf spot of Cucumis sativus (Cucurbitaceae), Mar. 1993, C.F. Hill, culture ex-type of A. cucumericola CBS 116438 = E.G.S. 41.057. Thailand, Chiang Mai, Royal project, from leaf spot of Solanum lycopersicum, 5 Nov. 2012, P.W. Crous, CPC 21620. Unknown, host unknown, before Apr. 1953, P.W. Brian, CBS 108.53 = No. 408P. USA, Indiana, Montgomery County, from leaf spot of Solanum lycopersicum, 23 Aug. 1995, E.G. Simmons, culture ex-type of A. tomatophila CBS 109156 = E.G.S. 42.156; Indiana, from leaf lesion of Solanum lycopersicum, Aug. 1996, E.G. Simmons, representative isolate of A. tomatophila CBS 116704 = E.G.S. 44.074; Louisiana, Baton Rouge, Louisiana State University Burden Research Plantation, from leaf lesion of Solanum lycopersicum var. cerasiforme, 2 Jul. 1997, R.G. Roberts, culture ex-type of A. subcylindrica CBS 109161 = E.G.S. 45.113; Louisiana, Avery Island, from leaf spot of Capsicum frutescens (Solanaceae), 1 Jul. 1997, R.G. Roberts, culture ex-type of A. tabasco CBS 116441 = E.G.S 45.108 = R.G.R. 97-52.

Notes: By synonymising A. cretica, A. cucumericola, A. subcylindrica, A. tabasco and A. tomatophila with A. linariae, the broad host range of this taxon now consists of Solanaceae, Cucurbitaceae and Scrophulariaceae species. The isolates CBS 108.53 and CBS 116704 did not sporulate on SNA after 3 wk of incubation.

Alternaria macrospora Zimm., Ber. Land-Forstw. Deutsch-Ostafrika 2: 24. 1904.

≡ Macrosporium macrosporum (Zimm.) Nishikado & Oshima, Agric. Res. (Kurashiki) 36: 391. 1944.

= Sporidesmium longipedicellatum Reichert, Bot. Jahrb. Syst. 56: 723. 1921.

≡ Alternaria longipedicellata (Reichert) Snowden, Rep. Dept. Agric. Uganda: 31. 1927 [1926].

Materials examined: Nigeria, from Gossypium sp. (Malvaceae), May 1929, Jones, CBS 106.29. USA, Arizona, from Gossypium barbadense (Malvaceae), before 1984, P.J. Cotty, culture epitype of A. macrospora CBS 117228 = E.G.S. 50.190 = ATCC 58172.

Notes: Isolate CBS 106.29 was preserved in the CBS collection as A. porri, but did not sporulate since 1978. Based on our molecular data this isolate belongs to A. macrospora, which, based on the same host, seems plausible.

Alternaria montanica E.G. Simmons & Robeson, CBS Biodiversity Ser. (Utrecht) 6: 178. 2007.

Material examined: USA, Montana, from Cirsium arvense (Asteraceae), before Apr. 1981, D.J. Robeson, culture ex-type of A. montanica CBS 121343 = E.G.S. 44.112 = IMI 257563.

Alternaria multirostrata E.G. Simmons & C.R. Jacks., Phytopathology 58: 1139. 1968.

Materials examined: USA, Georgia, Tifton, from floral bract of Richardia scabra (Rubiaceae), 1967, C.R. Jackson, culture ex-type of A. multirostrata CBS 712.68 = ATCC 18515 = IMI 135454 = MUCL 11722 = QM 8820 = VKM-F2997; Georgia, Tifton, from floral bract of Richardia scabra, 1967, C.R. Jackson, representative isolate of A. multirostrata CBS 713.68 = ATCC 18517 = IMI 135455 = MUCL 11715 = QM 8821.

Alternaria neoipomoeae M. Truter, Woudenb. & Crous, sp. nov. MycoBank MB808998. Fig. 19.

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Fig. 19

Alternaria neoipomoeae sp. nov. A. Disease symptoms on Ipomoeae batatas (Photo A.H. Thompson, ARC, South Africa). B–L. PPRI 11845: conidia and conidiophores. Scale bars = 10 μm.

Etymology: Named after its close phylogenetic relationship to A. ipomoeae.

Primary conidiophores solitary, simple, straight to slightly curved, septate, pale brown, (10–)23–59(–111) × (4–)5 μm, bearing a single, darkened, apical conidiogenous locus, which may produce 1–2 geniculate conidiogenous extensions. Conidia are mostly solitary but chains of two conidia can occur, conidium body olive-brown, smooth-walled with ornamented base, long ellipsoid to obclavate, (52–)66–77(–93) × (12–)14–16(–18) μm, with (7–)9(–12) transverse and (2–)3–4(–5) longitudinal septa. Up to four dark coloured eusepta can be formed during development; the conidial body is constricted near these eusepta. Conidia mostly have a septate, single to double, filamentous beak, triple beaks are observed but not common; apical and lateral secondary conidiophores can be formed. Beaks (54–)104–136(–200) μm long, ca. 2 μm diam throughout their length, and approx. 3 μm diam at the base. Sexual morph not observed.

Culture characteristics: After 7 d cultures on SNA flat, fimbriate, white to opaque; aerial mycelium sparse, fine felty, white, colonies reaching 60−65 mm diam; cultures on PCA flat, entire, grey-olivaceous with 2 dark and one lighter concentric circles and a pale olivaceous edge; aerial mycelium fine felty, pale olivaceous-grey, colonies reaching 55–60 mm diam; reverse four olivaceous-grey rings.

Materials examined: South Africa, Gauteng Province, Pretoria, ARC-Roodeplaat VOPI, from stem lesion of Ipomoea batatas (Convolvulaceae), 8 Jun. 2011, A. Thompson (holotype PREM 60981, culture ex-type PPRI 11845); North-West Province, Brits, from Ipomoea batatas, 25 Oct. 2007, C.D. Narayanin (paratype PREM 60982, culture ex-paratype PPRI 8990); Mpumalanga Province, Kwamahlanga, from Ipomoea batatas, between 2006 and 2008, C.D. Narayanin (paratype PREM 60983, culture ex-paratype PPRI 11847); Gauteng Province, Pretoria, ARC-Roodeplaat VOPI, from leaf lesion of Ipomoea batatas, Oct. 2013, A. Thompson (paratype PREM 60984, culture ex-paratype PPRI 13903).

Alternaria nitrimali E.G. Simmons & M.E. Palm, Mycotaxon 75: 93. 2000.

Material examined: Puerto Rico, Luquillo, from leaf spot of Solanum viarum (Solanaceae), 26 Feb. 1998, USDA-APHIS, culture ex-type of A. nitrimali CBS 109163 = E.G.S 46.151.

Alternaria novae-guineensis E.G. Simmons & C.F. Hill, CBS Biodiversity Ser. (Utrecht) 6: 350. 2007.

Materials examined: Papua New Guinea, from dried leaf of Citrus sp. (Rutaceae) imported to New Zealand, 1999, C.F. Hill, culture ex-type of A. novae-guineensis CBS 116120 = E.G.S. 47.198. South Africa, Gauteng, Pretoria, ARC-Roodeplaat VOPI, from leaves of Galinsoga parviflora (Asteraceae), 12 Jan. 2012, A. Thompson, PPRI 12171.

Alternaria obtecta E.G. Simmons, Mycotaxon 50: 250. 1994.

Materials examined: USA, California, Encinitas, from leaf of Euphorbia pulcherrima (Euphorbiaceae), Nov. 1994, C.F. Hill, representative isolate of A. obtecta CBS 117367 = E.G.S. 42.063; California, Encinitas, from Euphorbia pulcherrima (Euphorbiaceae), Nov. 1994, C.F. Hill, CBS 134278 = E.G.S. 42.064.

Alternaria paralinicola Woudenb. & Crous, sp. nov. MycoBank MB808999. Fig. 20.

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Fig. 20

Alternaria paralinicola sp. nov. CBS 116652: A–L. Conidia and conidiophores. Scale bars = 10 μm.

Etymology: Named after its close phylogenetic relationship to A. linicola.

Primary conidiophores solitary, simple, straight to slightly curved, septate, pale brown, (39–)64–82(–133) × (4–)5–6 μm, bearing a single, darkened, apical conidiogenous locus, but may produce geniculate conidiogenous extensions. Conidia are mostly solitary but chains of two conidia can occur, conidium body pale olive-brown, smooth-walled, narrowly ovoid, (31–)39–44(–58) × (8–)10–11(–15) μm, with (3–)5–6(–8) transverse septa and 0–1(–2) longitudinal septa. Dark coloured eusepta are formed during maturation. The conidial body is slightly constricted near the transverse septa. Some transverse blocks of cells can have a conspicuously different width in comparison with neighbouring segments, resulting in specific shape of the conidium body. Conidia mostly have a single, aseptate, unbranched, filamentous beak; double beaks are observed but not common; apical or lateral secondary conidiophores can be formed. Beaks (61–)114–135(–169) μm long, ca. 2 μm diam throughout their length. Sexual morph not observed.

Culture characteristics: After 7 d cultures on SNA flat, fimbriate, white to opaque; aerial mycelium sparse, white, colonies reaching 70–75 mm diam; cultures on PCA flat, entire, grey-olivaceous with four olivaceous clear concentric circles; aerial mycelium is fine felty, olivaceous, colonies reaching 70 mm diam; reverse shows five grey-olivaceous concentric circles.

Material examined: Canada, Manitoba, from seeds of cultivated Linum usitatissimum (Linaceae), 1996, M.E. Corlett (holotype CBS H-21740, culture ex-type CBS 116652 = E.G.S. 47.157 = DAOM 225747).

Note: Alternaria paralinicola, which was originally identified as A. linicola, differs on 16 nt positions in its RPB2 sequence from the other two A. linicola strains studied. Based on its RPB2 sequence it clusters with A. passiflorae.

Alternaria passiflorae J.H. Simmonds, Proc. Roy. Soc. Queensland. 49: 151. 1938. Fig. 21.

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Fig. 21

Alternaria passiflorae: conidia and conidiophores. A–B. CBS 117102. C–D. CBS 117103. E–F. CBS 116333. G–H. CBS 166.77. I–J. CBS 630.93. K–L. CBS 629.93. Scale bars = 10 μm.

= Alternaria hawaiiensis E.G. Simmons, Mycotaxon 46: 184. 1993.

= Alternaria gaurae E.G. Simmons & C.F. Hill, CBS Biodiversity Ser. (Utrecht) 6: 188. 2007.

Materials examined: New Zealand, from fruit of Passiflora edulis (Passifloraceae), 6 Feb. 1963, F.J. Mortin, representative isolate of A. passiflorae CBS 629.93 = E.G.S. 16.150 = QM 8458; Auckland, from fruit spot of Passiflora ligularis (Passifloraceae), Apr. 2004, C.F. Hill, representative isolate of A. passiflorae CBS 117102 = E.G.S. 51.165; Auckland, from leaf spot of Passiflora caerulea (Passifloraceae), Jul. 2004, C.F. Hill, representative isolate of A. passiflorae CBS 117103 = E.G.S. 52.032; Auckland, from leaf spot of Gaura lindheimeri (Onagraceae), May 2002, C.F. Hill, culture ex-type of A. gaurae CBS 116333 = E.G.S. 50.121; Waitakere, from leaf of Capsicum frutescens (Solanaceae), May 1975, CBS 166.77. USA, Hawaii, from Passiflora edulis, before Oct. 1968, M. Aragaki, culture ex-type of A. hawaiiensis CBS 630.93 = E.G.S. 29.020 = QM 9050.

Notes: By synonymising A. gaurae with A. passiflorae, and including CBS 166.77, formerly identified as A. solani, the host range of A. passiflorae has broadened to include Gaura sp. (Onagraceae) and Capsicum frutescens (Solanaceae).

Alternaria pipionipisi E.G. Simmons, CBS Biodiversity Ser. (Utrecht) 6: 302. 2007.

Materials examined: India, Andhra Pradesh, Hyderabad, from seed of Cajanus cajan (Fabaceae), before Feb. 1990, K.M. & Ch. Reddy, culture ex-type of A. pipionipisi CBS 116115 = E.G.S. 40.096 = IMI 340950. USA, California, Encinitas, from Euphorbia pulcherrima (Euphorbiaceae), Sep. 1994, C.F. Hill, CBS 134265 = E.G.S. 42.047; California, Encinitas, from Euphorbia pulcherrima, Sep. 1994, C.F. Hill, representative isolate of A. obtecta CBS 117365 = E.G.S. 42.048.

Alternaria porri (Ellis) Cif., J. Dept. Agric. Porto Rico 14: 30. 1930 [1929]. Fig. 22.

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Fig. 22

Alternaria porri: conidia and conidiophores. A–D. CBS 116698. E–H. CBS 116699. I–L. CBS 116649. Scale bars = 10 μm.

Basionym: Macrosporium porri Ellis, Grevillea 8 (no. 45): 12. 1879.

≡ Alternaria porri (Ellis) Sawada, Rep. Dept. Agric. Gov. Res. Inst. Formosa, 61: 92. 1930.

Type: (Lectotype, designated in Simmons 2007) NY, Ellis Collection: on leaves of Allium porrum, Newfield, N.J. Sept. 78.

Materials examined: USA, Nebraska, Lincoln, from leaf of Allium cepa (Amaryllidaceae), 1965, D.S. Meredith, representative isolate of A. allii CBS 116649 = E.G.S. 17.082 = QM 8613; New York, Ithaca, from leaf of Allium cepa, 1996, M.J. Yáñes Morales, representative isolate of A. porri CBS 116698 = E.G.S. 48.147; New York, Orange County, from leaf of Allium cepa, 1996, M.J. Yáñes Morales (epitype designated here CBS H-21746, MBT178117, culture ex-epitype CBS 116699 = E.G.S. 48.152).

Alternaria protenta E.G. Simmons, Mycotaxon 25: 207. 1986. Fig. 23.

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Fig. 23

Alternaria protenta: conidia and conidiophores. A–B. CBS 116696. C–D. CBS 116697. E–G. CBS 116643. H–J. CBS 116651. K–M. CBS 121342. N–P. CBS 347.79. Scale bars = 10 μm.

= Alternaria pulcherrimae T.Y. Zhang & J.C. David, Mycosystema 8-9: 110. 1996.

= Alternaria hordeiseminis E.G. Simmons & G.F. Laundon, CBS Biodiversity Ser. (Utrecht) 6: 150. 2007.

Materials examined: Australia, Queensland, Brisbane, Chapel Hill, from Euphorbia pulcherrimae (Euphorbiaceae), 25 Aug. 1986, J.L. Alcorn, representative isolate of A. pulcherrimae CBS 121342 = E.G.S. 42.122 = IMI 310506. Israel, from Helianthus annuus (Asteraceae), 1996, collector unknown, representative isolate of A. protenta CBS 116697 = E.G.S. 45.024 = IMI 372957; from Helianthus annuus, 1996, collector unknown, representative isolate of A. protenta CBS 116696 = E.G.S. 45.023 = IMI 372955. New Zealand, Hastings, from Solanum tuberosum (Solanaceae), Mar. 1997, C.F. Hill, representative isolate of A. solani CBS 135189 = E.G.S. 45.053; Levin, from fruit rot of Solanum lycopersicum (Solanaceae), before Jul. 1979, G.F. Laundon, CBS 347.79 = E.G.S. 44.091 = ATCC 38569 = LEV 14726; Palmerston North, from seed of Hordeum vulgare (Gramineae), Jul. 1977, G.F. Laundon, culture ex-type of A. hordeiseminis CBS 116437 = E.G.S. 32.076 = CBS 116443 = E.G.S. 46.163. USA, California, Siskiyou, from Solanum tuberosum, 1996, D. Fogle, representative isolate of A. solani CBS 116651 = E.G.S. 45.020.

Notes: By synonymising A. pulcherrimae and A. hordeiseminis with A. protenta and including three isolates formerly identified as A. solani (CBS 347.79, 116651 and 135189), the host range of A. protenta has expanded extensively. It now comprises plants from the Asteraceae, Euphorbiaceae, Gramineae and Solanaceae. Based on molecular (and morphological) data, A. protenta is closely related to A. solani, and these two species can only be distinguished based on 9 nt differences in their RPB2 sequences (see RPB2 alignment in TreeBASE).

Alternaria pseudorostrata E.G. Simmons, Mycotaxon 57: 398. 1996.

Material examined: USA, California, Encinitas, from Euphorbia pulcherrimae (Euphorbiaceae), Dec. 1994, C.F. Hill, culture ex-type of A. pseudorostrata CBS 119411 = E.G.S. 42.060.

Alternaria ranunculi E.G. Simmons, CBS Biodiversity Ser. (Utrecht) 6: 212. 2007.

Material examined: Israel, Palestine, from seed of Ranunculus asiaticus (Ranunculaceae), 10 Apr. 1984, collector unknown, culture ex-type of A. ranunculi CBS 116330 = E.G.S. 38.039 = IMI 285697.

Alternaria ricini (Yoshii) Hansf., Proc. Linn. Soc. Lond. : 53. 1943.

Basionym: Macrosporium ricini Yoshii, Bult. Sci. Fak. Terk. Kjusu Imp. Univ. 3(4): 327. 1929.

Type: (Lectotype, designated in Simmons 1994) BPI 445446, Macrosporium ricini, Japan, Fukuoka, Ricinus communis, July 1928.

Materials examined: Italy, Sardinia, Sasseri, from Ricinus communis (Euphorbiaceae), before Aug. 1986, J.A. von Arx, CBS 353.86. Japan, Ricinus communis, deposited Feb. 1931 by K. Nakata (epitype designated here CBS H-21747, MBT178118, culture ex-epitype CBS 215.31). USA, Virginia, Holland, from leaf of Ricinus communis, 9 Aug. 1954, C.A. Thomas, representative isolate of A. ricini CBS 117361 = E.G.S. 06.181.

Alternaria rostellata E.G. Simmons, Mycotaxon 57: 401. 1996.

Material examined: USA, California, Encinitas, from leaf of Euphorbia pulcherrimae (Euphorbiaceae), Jan. 1995, C.F. Hill, culture ex-type of A. rostellata CBS 117366 = E.G.S. 42.061.

Alternaria scorzonerae (Aderh.) Loer., Netherlands J. Pl. Pathol. 90(1): 37. 1984.

Basionym: Sporidesmium scorzonerae Aderh., Arbeiten Kaiserl. Biol. Anst. Land-Forstw. 3: 439. 1903.

= Alternaria linicola J.W. Groves & Skolko, Canad. J. Res., Sect. C, Bot. Sci. 22: 223. 1944.

= Alternaria linicola Neerg, Danish species of Alternaria & Stemphylium: 302. 1945. (nom. illegit., Art. 53.1)

Type: (Lectotype, designated in Simmons 1997) Aderhold, Arbeiten Kaiserl. Biol. Anst. Land-Forstw. 3: 440. fig. w/o number. 1903.

Materials examined: Netherlands, Reusel, from leaf spot of Scorzonera hispanica (Asteraceae), 1982, W.M. Loerakker (epitype designated here CBS H-21748, MBT178119, culture ex-epitype CBS 478.83 = E.G.S. 38.011). UK, Scotland, from Linum usitatissimum (Linaceae), 22 Nov. 1945, J.W. Groves, CBS 103.46; Derbyshire, from seed of Linum usitatissimum, 1983, C. Nicholls, representative isolate of A. linicola CBS 116703 = E.G.S. 36.110 = IMI 274549.

Notes: None of the three isolates sporulated on SNA or PCA after 3 wk of incubation, also not after scarification. Corlett & Corlett (1999) already stated that, after sub-cultivation, A. linicola sporulates poorly, or not at all. By synonymising A. linicola with A. scorzonerae, the host range of A. scorzonerae is expanded to include Linum usitatissimum (Linaceae).

Alternaria sennae Woudenb. & Crous, sp. nov. MycoBank MB809000. Fig. 24.

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Fig. 24

Alternaria sennae sp. nov. CBS 477.81: A–L. Conidia and conidiophores. Scale bars = 10 μm.

Etymology: Named after the host genus on which it occurs, Senna.

Primary conidiophores solitary, simple, straight to slightly curved, septate, dark brown with a hyaline tip, (43–)67–81(–108) × (5–)6(–7) μm, bearing a single, darkened, apical conidiogenous locus, but may produce geniculate conidiogenous extensions. Conidia solitary, conidium body pale olive-brown, smooth-walled, narrowly ovoid, (46–)55–62(–69) × (8–)10–12(–14) μm, with (7–)7–8(–10) transverse distosepta and (1–)2–3(–4) longitudinal septa. The conidial body can be slightly constricted near some transverse septa. Conidia have a single, aseptate, filamentous beak, which occasionally branches once; basal lateral secondary conidiophores can be formed. Beaks (38–)99–163(–314) μm long, ca. 2 μm diam. Sexual morph not observed.

Culture characteristics: After 7 d cultures on SNA flat, fimbriate, white to opaque with two olivaceous concentric circles; aerial mycelium sparse, white, floccose, colonies reaching 35−40 mm diam; cultures on PCA flat, undulate, white with grey-olivaceous zones; aerial mycelium felty, pale olivaceous-grey, colonies reaching 50–55 mm diam; reverse with pale olivaceous-grey zones.

Material examined: India, Uttar Pradesh, Gorakhpur, from leaf of Senna corymbosa (Fabaceae), 10 Apr. 1981, R.P. Verma (holotype CBS H-21741, culture ex-type CBS 477.81 = E.G.S. 34.030 = IMI 257253).

Alternaria sesami (E. Kawam.) Mohanty & Behera, Curr. Sci. 27: 493. 1958.

Basionym: Macrosporium sesami E. Kawam., Fungi 1: 27. 1931.

Materials examined: Egypt, from Sesamum indicum (Pedaliaceae), 1972, S.B. Mathur, CBS 240.73. India, from seedlings of Sesamum indicum, Dec. 1959, E.E. Leppik, representative isolate CBS 115264 = CBS 117214 = E.G.S. 13.027.

Alternaria sidae E.G. Simmons, Mycotaxon 88: 202. 2003.

Material examined: Kiribati, Phoenix islands, Canton Island, from leaf spot of Sida fallax (Malvaceae), 11 Feb. 1958, O. & I. Degener, culture ex-type of A. sidae CBS 117730 = E.G.S. 12.129.

Alternaria silybi Gannibal, Mycotaxon 114: 110. 2011.

Materials examined: Russia, Vladivostok, Trudovoe, from leaf lesion of Silybum marianum (Asteraceae), 1 Sep. 2006, Ph. B. Gannibal, culture ex-type of A. silybi CBS 134092 = VKM F-4109; Vladivostok, Trudovoe, from leaf lesion of Silybum marianum, 1 Sep. 2006, Ph. B. Gannibal, CBS 134094 = VKM F-4118; Vladivostok, Botanical Garden-Institute, from leaf lesion of Silybum marianum, 6 Sep. 2006, Ph. B. Gannibal, CBS 134093 = VKM F-4117.

Alternaria solani Sorauer, Z. Pflanzenkrankh. Pflanzenschutz 6: 6. 1896. Fig. 25.

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Fig. 25

Alternaria solani. A. Disease symptoms on Solanum tuberosum (Photo J.E. van der Waals, University of Pretoria, South Africa). B–H. Conidia and conidiophores. B–D. CBS 109157. E–H. CBS 116442. Scale bars = 10 μm.

= Macrosporium solani Ellis & G. Martin, Amer. Naturalist 16(12): 1003. 1882 (non M. solani Cooke, 1883)

≡ Alternaria solani (Ellis & G. Martin) L.R. Jones & Grout, Vermont Agric. Exp. Sta. Annual Rep. 9: 86. 1899. (nom. illegit., Art. 53.1)

≡ Alternaria americana Sawada, Rep. Dept. Agric. Gov. Res. Inst. Formosa 51:117. 1931. (nom. nov. for A. solani (Ellis & G. Martin) L.R. Jones & Grout (1899), non A. solani Sorauer (1896))

≡ Alternaria porri f. sp. solani (Ellis & G. Martin) Neerg, Danish species of Alternaria & Stemphylium: 260. 1945.

= Sporidesmium solani-varians Vañha, Naturwiss. Z. Forst- Landw. 2: 117. 1904.

= Alternaria danida E.G. Simmons, Mycotaxon 65: 78. 1997.

= Alternaria viciae-fabae E.G. Simmons & G.F. Laundon, CBS Biodiversity Ser. (Utrecht) 6: 234. 2007.

Materials examined: Italy, from seed of Ageratum houstonianum (Asteraceae), 27 Aug. 1941, P. Neergaard, culture ex-type of A. danida CBS 111.44 = E.G.S. 07.029 = QM 1772. New Zealand, from Vicia faba (Fabaceae), Jun. 1979, G.F. Laundon, culture ex-type of A. viciae-fabae CBS 116442 = E.G.S. 46.162 = ICMP 10242. Unknown, from leaf spot of Solanum aviculare (Solanaceae), before May 1941, P. Neergaard, CBS 111.41; unknown host, before Nov. 1921, isolated by Künkel, CBS 106.21. USA, Washington, Douglas County, from leaf spot of Solanum tuberosum (Solanaceae), 25 Aug. 1996, E.G. Simmons, representative isolate of A. solani CBS 109157 = E.G.S. 44.098.

Notes: By synonymising A. danida and A. viciae-fabae with A. solani, the host range of this pathogen has expanded to include Asteraceae and Fabaceae host plants. The isolates CBS 106.21 and CBS 111.44 did not sporulate after 3 wk of incubation on SNA (both were already labelled as sterile in the CBS collection database). Isolate CBS 111.41 did sporulate, but the spore formation was atypical.

Alternaria solani-nigri R. Dubey, S.K. Singh & Kamal [as “solani-nigrii”], Microbiol. Res. 154: 120. 1999. Fig. 26.

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Fig. 26

Alternaria solani-nigri: conidia and conidiophores. A–B. CBS 113403. C–D. CBS 116447. E–G. CBS 109155. H–I. CBS 116334. J–K. CBS 121347. L–M. CBS 116332. N–P. CBS 117101. Scale bars = 10 μm.

= Alternaria cyphomandrae E.G. Simmons, Mycotaxon 75: 86. 2000.

= Alternaria ascaloniae E.G. Simmons & C.F. Hill, CBS Biodiversity Ser. (Utrecht) 6: 168. 2007.

= Alternaria beticola E.G. Simmons & C.F. Hill, CBS Biodiversity Ser. (Utrecht) 6: 170. 2007.

= Alternaria glyceriae E.G. Simmons & C.F. Hill, CBS Biodiversity Ser. (Utrecht) 6: 148. 2007.

= Alternaria herbiculinae E.G. Simmons, CBS Biodiversity Ser. (Utrecht) 6: 166. 2007.

Materials examined: New Zealand, Canterbury, Ashburton, from leaf lesion of Beta vulgaris (Chenopodiaceae), Jul. 1999, B. Alexander, culture ex-type of A. beticola CBS 116447 = E.G.S. 47.196; Hastings, from leaf spot of Allium ascalonicum (Amaryllidaceae), Oct. 1997, C.F. Hill, culture ex-type of A. ascaloniae CBS 121347 = E.G.S 46.052; New Plymouth, from fruit of Cyphomandra betacea (Solanaceae), May 1991, C.F. Hill, culture ex-type of A. cyphomandrae CBS 109155 = E.G.S. 40.058; Taranaki, Otaki, from stunted Petroselinum crispum (Apiaceae), 14 Jun. 2001, J.B. Wong, culture ex-type of A. herbiculinae CBS 116332 = E.G.S. 49.180; Waikato, Kopuku, from leaf spot of Glyceria maxima (Gramineae), Apr. 2003, C.F. Hill, culture ex-type of A. glyceriae CBS 116334 = E.G.S. 51.107; Waikato, Whangamarino swamp, from leaf spot of Solanum nigrum (Solanaceae), 21 Jun. 2003, C.F. Hill, representative isolate of A. solani-nigri CBS 113403 = E.G.S. 51.106 = CPC 10620; Waikato, Whangamarino swamp, from leaf spot of Solanum nigrum, 6 Feb. 2003, C.F. Hill, representative isolate of A. solani-nigri CBS 117101 = E.G.S. 51.032.

Notes: By synonymising these five Alternaria species with A. solani-nigri, this becomes a species with a broad host range found on Amaryllidaceae, Apiaceae, Chenopodiaceae, Gramineae and Solanaceae. All studied specimens originate from New Zealand, but the holotype of A. solani-nigri was described from India. The five sequenced genes are 100 % identical between all the specimens studied.

Alternaria steviae Ishiba, T. Yokoy. & Tani, Ann. Phytopathol. Soc. Japan 48(1): 46. 1982.

Materials examined: Japan, Kagawa, Kida-gun, Miki-cho, Ikenobe, from leaf spot of Stevia rebaudiana (Asteraceae), CBS 631.88 = IFO 31212; Kagawa, Kida-gun, Miki-cho, Ikenobe, from leaf spot of Stevia rebaudiana, Jun. 1980, CBS 632.88 = IFO 31183; Kagawa, Zentsuji, Harada-cho, from leaf spot of Stevia rebaudiana, Aug. 1978, C. Ishiba, culture ex-type of A. steviae CBS 117362 = IFO 31182 = E.G.S. 37.019.

Alternaria tagetica S.K. Shome & Mustafee, Curr. Sci. 35: 370. 1966.

Materials examined: UK, from seed of Tagetes sp. (Asteraceae), before May 1979, G.S. Taylor, CBS 297.79; from seed of Tagetes sp., before May 1979, G.S. Taylor, CBS 298.79; England, Manchester, from seed of Tagetes erecta (Asteraceae), before Apr. 1980, G.S. Taylor, representative isolate of A. tagetica CBS 479.81 = E.G.S. 33.081. USA, Ohio, Butler County, Oxford, from leaf of cultivated Tagetes sp., 14 Jun. 1996, M.A. Vincent, representative isolate of A. tagetica CBS 117217 = E.G.S 44.045; South Carolina, Clemson, from seed of Tagetes sp., before Mar. 1981, E. Smallwood Hotchkiss, representative isolate of A. tagetica CBS 480.81 = E.G.S. 33.184.

Alternaria thunbergiae E.G. Simmons & Alcorn, CBS Biodiversity Ser. (Utrecht) 6: 136. 2007. Fig. 27.

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Fig. 27

Alternaria thunbergiae: conidia and conidiophores. A–C. CBS 116331. D–E. CBS 122597. F–H. CBS 120986. Scale bars = 10 μm.

= Alternaria iranica E.G. Simmons & Ghosta, CBS Biodiversity Ser. (Utrecht) 6: 122. 2007.

Materials examined: Australia, Queensland, Brisbane, Chapel Hill, from leaf spot of Thunbergia alata (Acanthaceae), 6 Feb. 1986, J.L. Alcorn, culture ex-type of A. thunbergiae CBS 116331 = E.G.S. 41.073 = BRIP 14963. Iran, Miandoab, from leaf of Allium cepa (Amaryllidaceae), 13 Sep. 2001, Y. Ghosta, culture ex-type of A. iranica CBS 120986 = E.G.S. 51.075. New Zealand, Auckland, Mangere, Tidal Road, from Thunbergia alata, 4 Jun. 2001, C.F. Hill, CBS 122597.

Notes: By synonymising A. iranica with A. thunbergiae, the host range of this taxon has expanded to include Allium cepa. The five sequenced genes are 100 % identical between the ex-type strains of A. thunbergiae and A. iranica. As both species were originally described in the same publication, there is no case for nomenclatural priority. Therefore we chose to synonymise A. iranica under A. thunbergiae because A. thunbergiae is more commonly used in literature (Leahy 1992, Melo et al. 2009).

Alternaria tillandsiae E.G. Simmons & C.F. Hill, CBS Biodiversity Ser. (Utrecht) 6: 314. 2007.

Material examined: USA, from Tillandsia usneoides (Bromeliaceae), Dec. 1995, B. Milnes, culture ex-type of A. tillandsiae CBS 116116 = E.G.S. 43.074.

Alternaria tropica E.G. Simmons, Mycotaxon 46: 187. 1993.

Materials examined: USA, Florida, Homestead, from fruit of Passiflora edulis (Passifloraceae), May 1990, R.T. McMillan Jr., culture ex-type of A. tropica CBS 631.93 = E.G.S. 39.126; Florida, Homestead, from fruit of Passiflora edulis, May 1990, R.T. McMillan Jr., representative isolate of A. tropica CBS 117216 = E.G.S. 39.125.

Alternaria venezuelensis E.G. Simmons & Rumbos, CBS Biodiversity Ser. (Utrecht) 6: 128. 2007.

Material examined: Venezuela, Maracay, from leaf spot of Phaseolus vulgaris (Fabaceae), before Oct. 1999, R. Rumbos, culture ex-type of A. venezuelensis CBS 116121 = E.G.S. 48.065.

Alternaria zinniae M.B. Ellis, Mycol. Pap. 131: 22. 1972.

= Alternaria zinniae H. Pape, Angew. Bot. 24: 61. 1942. (nom. inval., Art. 36.1)

Materials examined: Hungary, from seed of Callistephus chinensis (Asteraceae), 12 Aug. 1942, P. Neergaard, CBS 118.44. Italy, Sardinia, Sasseri, from Zinnia elegans (Asteraceae), 18 Oct. 1958, U. Prota, CBS 117.59. Netherlands, Huizum, from leaf of Zinnia sp., 27 Jul. 1948, A. Jaarsveld, CBS 107.48. New Zealand, Auckland, Royal Oak, from leaf spot of Zinnia elegans, May 1996, C.F. Hill, representative isolate of A. zinniae CBS 117223 = E.G.S. 44.035. UK, from seed of Zinnia sp., 1979, G.S. Taylor, CBS 299.79; from seed of Zinnia sp., 1979, G.S. Taylor, CBS 300.79. Unknown, from Zinnia elegans, summer 1961, Smith, CBS 108.61.

Section Euphorbiicola Woudenb. & Crous, sect. nov. MycoBank MB809001. Fig. 28

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Fig. 28

Alternaria section Euphorbiicola: conidia and conidiophores. A–G. Alternaria limicola. H–P. Alternaria euphorbiicola. A–D. CBS 117360. E–G. CBS 483.90. H–J. CBS 198.86. K–M. CBS 119410. N–P. CBS 133874. Scale bars = 10 μm.

Type species: Alternaria euphorbiicola E.G. Simmons & Engelhard.

Section Euphorbiicola is characterised by ovoid, obclavate, medium to large conidia that are disto- and euseptate, in short to moderately long chains, with no or a simple long beak in the terminal conidia. Conidia contain multiple transverse and some longitudinal septa and are slightly constricted near some transverse septa. Short to long, broad, apical, and sometimes lateral, secondary conidiophores are formed.

Note: The new Alternaria sect. Euphorbiicola can be easily distinguished from sect. Porri based on the formation of conidia in chains in sect. Euphorbiicola.

Alternaria euphorbiicola E.G. Simmons & Engelhard, Mycotaxon 25: 196. 1986.

≡ Macrosporium euphorbiae Reichert, Bot. Jahrb. Syst. 56: 723. 1921. Non Macrosporium euphorbiae Bartholomew 1908. (nom. illegit., Art 53.1).

Materials examined: USA, Florida, from Euphorbia pulcherrima (Euphorbiaceae), 1985, A.W. Engelhard, CBS 198.86 = E.G.S. 38.082; Hawaii, Oahu, from Euphorbia pulcherrima, Mar. 1984, M. Aragaki, representative isolate CBS 119410 = E.G.S. 41.029; Louisiana, from Euphorbia hyssopifolia (Euphorbiaceae), 1986, L. Walker, CBS 133874 = E.G.S 38.191.

Alternaria limicola E.G. Simmons & M.E. Palm, Mycotaxon 37: 82. 1990.

Materials examined: Mexico, Colima, from leaf of Citrus aurantiifolia (Rutaceae), May 1989, M. Palm, culture ex-type of A. limicola CBS 483.90 = E.G.S. 39.070; Jalisco, from Citrus sp., Sep. 1995, M. Palm, representative isolate CBS 117360 = E.G.S. 43.009.

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Discussion

In the present phylogenetic study aiming to delimit Alternaria species in sect. Porri, we reduced the 82 known morphospecies in this section to 63 based on our polyphasic approach. Some important plant pathogens have now been assigned to specific clades in the phylogenetic tree and correlated with their distinct morphology, which will aid plant pathologists to identify their newly collected isolates.

The 10 isolates named A. solani at the onset of this study cluster within five different species-clades, and only three of them retain the name A. solani. This is not surprising, as almost all large-spored, narrow-beaked Alternaria strains hitherto isolated from Solanaceae were called A. solani, following the concept of M.B. Ellis (1971). Simmons (2000) already noted that early blight of tomato is actually caused by A. tomatophila rather than A. solani, and also described two additional species on tomato, A. cretica and A. subcylindrica. These tomato pathogens all cluster in one clade based on our phylogenetic analysis, which also includes the ex-type strain of A. linariae. The basionym of A. linariae, A. anagallidis var. linariae, is the oldest name in this cluster, which therefore applies to this clade mainly represented by tomato pathogens. When Neergaard (1945) described this species he found the fungus on seeds and seedlings with damping-off symptoms from Linaria marroccana (Scrophulariaceae), Antirrhinum majus (Scrophulariaceae) and on a healthy seedling of Papaver rhoeas (Papaveraceae). His pathogenicity tests (Neergaard 1945) showed that A. linariae could also attack Brassica oleracea (Brassicaceae), Solanum lycopersicum (Solanaceae), Lactuca sativa (Asteraceae), Godetia hybrida (Onagraceae), Nicotiana affinis (Solanaceae) and Papaver paeoniflorum (Papaveraceae), indicating a very broad host range. The isolates included in this study also show that, besides its broad host range, A. linariae is also widespread, found in Europe, USA, New Zealand and Asia. Three other isolates formerly identified as A. solani, including a former representative isolate used by Simmons (2007), cluster with A. protenta, an Alternaria species originally described from Helianthus annuus (Asteraceae). CBS 116651 is mentioned as a representative strain of A. solani by Simmons (2007), but he later expressed doubt as to the identity of this isolate (Simmons pers. comm.). The host range of A. protenta has expanded extensively, now comprising plants from the Asteraceae, Euphorbiaceae, Gramineae and Solanaceae. A pathogenicity test performed on A. protenta isolated from sunflower seed (Wu & Wu 2003) concluded that sunflower was the only susceptible host among the 10 host plants tested. One of the host plants tested was Solanum lycopersicum, which we include as host of A. protenta. However, the authors did not clearly state how the A. protenta isolates, which they only found on seed of one out of seven cultivars of sunflower seeds tested, were identified. The manuscript also lacks molecular data, which could affirm their identification of A. protenta. To our knowledge, no pathogenicity tests have thus far been performed with the species synonymised under A. protenta, A. hordeiseminis or A. pulcherrimae. Based on molecular and morphological data, A. protenta is closely related to A. solani, and these two species can only be distinguished by the 9 nt differences in their RPB2 sequences. To confirm the potato pathogen clade, called A. solani, we sequenced the RPB2 region of multiple isolates collected from Solanum tuberosum, which are present in E.G. Simmons collection, now deposited at the CBS. Almost all (22/24 strains) cluster within the now recognised A. solani species clade (data not shown). The ex-type strain of A. danida (CBS 111.44), now a synonym of A. solani, was originally deposited in the CBS collection by P. Neergaard as A. porri f. sp. solani. Pathogenicity tests performed on this strain (Neergaard 1945) showed that it could attack hosts from several plant families [e.g. Allium cepa (Amaryllidaceae), Brassica oleracea (Brassicaceae), Solanum lycopersicum (Solanaceae) and Lactuca sativa (Asteraceae)], indicating a very broad host range. Our sequences of A. danida differ from those deposited in GenBank by Lawrence et al. (2013), and therefore we repeated the cultivation and DNA extraction to confirm our results and the resulting synonymy with A. solani. Although the other large-spored, long-beaked Alternaria species described from potato, A. grandis (Simmons 2000), differs only by 1 nt in its GAPDH sequence (position 99, T instead of C, see locus alignment in TreeBASE) within the 2 722 positions used in the phylogeny, we did not synonymise A. grandis under A. solani. The two isolates included, CBS 109158 and CBS 116695, have substantially larger conidia than the other A. solani isolates, and a recently published study revealed that A. solani (CBS 109157) and A. grandis (CBS 109158) differ on 8 out of 770 nt in their calmodulin sequence (Gannibal et al. 2014).

The oldest large-spored onion pathogens, A. porri and A. allii, form two closely related but distinct clades, which only differ based on 8 nt in their RPB2 sequences (see locus alignment in TreeBASE). The three newer species described from Allium, A. ascaloniae, A. iranica and A. vanuatuensis (Simmons 2007), are all synonymised with other species. Alternaria ascaloniae is synonymised under A. solani-nigri, a species with a broad host range, mainly found in New Zealand. To our knowledge, no pathogenicity tests have been performed with the species now placed in synonomy with A. solani-nigri, which could affirm the broad host range for this species. Alternaria iranica is synonymised under A. thunbergiae, known as the causative agent of Alternaria leaf spot on Thunbergia (Leahy 1992), reported from Australia, USA and Brazil. Alternaria vanuatuensis clusters in the Allium clade, comprising A. allii and A. porri. Based on the sequence data generated here, it is synonymised under A. allii. According to Simmons (2007), the conidia of A. allii are distinguishable from those of A. porri and other large-spored species known on Allium, based on their multiple beaks and branches. However, the representative isolates of A. allii used by Simmons (2007) do not cluster in a single clade; CBS 116649 clusters with the two A. porri representative isolates. On the other hand, A. vanuatuensis is described as a single-beaked species, but clusters with the A. allii isolate deposited in the CBS collection by J.A.B. Nolla on 27 December 1927 as A. allii sp. nov. (CBS 107.28, recognised as the ex-type strain here). Simmons obtained this isolate from the CBS in February 2000 (E.G.S. 48.084), but was unable to induce sporulation. We observed few conidia, but these were only single-beaked. Unfortunately we could not induce CBS 116701 to sporulate, which leaves us at odds with Simmons's notes, with only single- to double-beaked conidia in the A. allii clade, and double- to triple-beaked conidia in the A. porri clade. The number of beaks and branches from the Allium isolates therefore is not suitable to make a distinction between the two major Allium species. The species can be easily differentiated on the basis of sequence data of the RPB2 gene region generated in this study.

Based on morphology, four large-spored Alternaria species with long beaks were described as Passifloraceae pathogens. Our phylogeny only supports three of these: A. tropica, A. aragakii and the more common A. passiflorae. The fourth species, A. hawaiiensis, is synonymised under A. passiflorae based on sequence data. Simmons (2007) described A. hawaiiensis as a new species lacking multiple beaks, which is a characteristic of A. passiflorae. Our sequence data led us to conclude that this characteristic is not suitable for species delimitation, which we also concluded from the data of the onion pathogens, A. allii, A. vanuatuensis and A. porri. The clustering of two isolates within our A. passiflorae clade, which originate from different host families (Onagraceae and Solanaceae), renders A. passiflorae as unspecific to Passifloraceae.

An ongoing study in South Africa on sweet potato pathogens reveals multiple Alternaria species on this host associated with blight symptoms on leaves, petioles, and stems. In addition to the known pathogen of sweet potato, A. bataticola, three other pathogenic species are delineated of which two are newly described as A. ipomoeae and A. neoipomoea. A new unknown Alternaria pathogen, causing sweet potato stem blight in Ethiopia, was reported by van Bruggen in 1984. This isolate (CBS 219.79) was sent to the CBS for identification, but the author did not agree with the morphological identification made at that time as A. cucumerina, a name under which it was still stored in the CBS collection. Our data indicate that this pathogen, which also is found in stem lesions of Ipomoea batatas in South Africa, should be recognised as a new species, now named A. ipomoeae. Most isolates from South Africa however cluster in a clade close to A. ipomoeae, now named A. neoipomoea, which can clearly be distinguished from A. ipomoeae morphologically and by sequence data. Two more isolates from sweet potato in South Africa are identified as A. argyroxiphii, an Alternaria species originally described from Argyroxiphium sp. This finding is a new host report for A. argyroxiphii, and a first report of the fungus from South Africa.

Based on the sequence data generated in this study, A. euphorbiicola and A. limicola clearly separate from the other species in sect. Porri (Fig. 1). This separation is supported by morphological differences, and we therefore propose the new section, sect. Euphorbiicola. However, when we examined the phylogeny displaying the neighbouring sections of sect. Porri (Fig. 2), questions arose concerning sect. Gypsophilae and sect. Radicina. These two sections display almost similar branch length differences within the respective sections, comparable to what sect. Porri displays with sect. Euphorbiicola. An additional character of sect. Gypsophilae and sect. Radicina is that the species within these sections share the same host family, respectively Caryophyllaceae and Apiaceae. We therefore choose to retain these sections at present, but additional molecular and morphological studies could eventually lead to the recognition of additional sections.

The present polyphasic approach displays the current species delimitation in Alternaria sect. Porri. We recognise 63 Alternaria species in this section with medium to large conidia and a long (filamentous) beak, which can be distinguished based on molecular data. Not all species distinctions are 100 % clear based on DNA data only; nevertheless, we tried to be consistent in synonymising or not synonymising species: the number of genes with nt differences and the number of nt differences are taken into account, together with the morphology, host, country and time of isolation. All Alternaria isolates currently stored in the CBS collection, which cluster within sect. Porri based on their gene sequences, were included in our study. Some species, however, are under-sampled, which results in some uncertainty in keeping isolates as separate species or reducing them to synonymy. Although we attempted to use the available data as best as possible, with the inclusion of additional isolates some uncertain species boundaries are bound to be better resolved.

The finding of the third species on potato (A. protenta) is a good example of the importance of fungal systematics. Multiple manuscripts report on the high level of genetic variability observed among A. solani isolates (van der Waals et al. 2004; Lourenco et al. 2011, Leiminger et al. 2013) and based on secondary metabolite profiling A. solani isolates cluster in two distinct groups (Andersen et al. 2008). Furthermore, two genotypes are described based on the cytochrome b gene structure of A. solani isolates (Leiminger et al. 2014), which is an important gene in fungicide resistance. However, our study indicates that previous reports could actually be dealing with three (or more) different species. Without knowing the correct identity of your pathogen, many incorrect conclusions can be drawn about diversity, evolutionary mechanisms, host range, and options for disease control.

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Acknowledgements

This research was supported by the Dutch Ministry of Education, Culture and Science through an endowment of the FES programme “Making the tree of life work”.

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Footnotes

Peer review under responsibility of CBS-KNAW Fungal Biodiversity Centre.

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glyceraldehyde-3-phosphate dehydrogenase (phosphorylating)(UniProt - A0A0A0WBA0)
glyceraldehyde-3-phosphate dehydrogenase (phosphorylating)(UniProt - A0A0A0WBA8)
glyceraldehyde-3-phosphate dehydrogenase (phosphorylating)(UniProt - A0A0A0WBL2)
glyceraldehyde-3-phosphate dehydrogenase (phosphorylating)(UniProt - A0A0A0WBK2)
glyceraldehyde-3-phosphate dehydrogenase (phosphorylating)(UniProt - A0A0A0WBJ0)

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Alternaria anagallidis partial glyceraldehyde-3-phosphate dehydrogenase(ENA - AIW81585)
Alternaria anagallidis partial glyceraldehyde-3-phosphate dehydrogenase(ENA - AIW81586)
Alternaria allii partial glyceraldehyde-3-phosphate dehydrogenase(ENA - AIW81580)
Alternaria allii partial glyceraldehyde-3-phosphate dehydrogenase(ENA - AIW81582)
Alternaria acalyphicola partial glyceraldehyde-3-phosphate dehydrogenase(ENA - AIW81577)

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Large-spored Alternaria pathogens in section Porri disentangled.

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Large-spored Alternaria pathogens in section Porri disentangled

J.H.C. Woudenberg

M. Truter

J.Z. Groenewald

P.W. Crous

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Introduction

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