Based on techniques
Detection of RB1 Gene Copy Number Variations Using a Multiplex Ligation-Dependent Probe Amplification Method
,
2018,
Springer Protocols
Springer Protocols
Epigenetic Barcodes for Detection of Adulterated Plants and Plant-Derived Products
Authors:
Matteo Busconi
2
,
2
,
Giovanna Soffritti
2
,
Marcelino De Los Mozos Pascual
1
,
José Antonio Fernandez
3
Matteo Busconi
2
,
2
,
Giovanna Soffritti
2
,
Marcelino De Los Mozos Pascual
1
,
José Antonio Fernandez
3
Series: Methods In Molecular Biology > Book: Plant Epigenetics and Epigenomics
Protocol | DOI: 10.1007/978-1-0716-0179-2_16
Affiliations:
Less
- Centro de Investigación Agroforestal de Albaladejito, Instituto Regional de Investigación y Desartrollo Agroalimentario y Forestal, Cuenca, Spain
- Faculty of Agriculture, Food and Environmental Sciences, Research Center BioDNA, Università Cattolica del Sacro Cuore, Piacenza, Italy
- IDR-Biotechnology and Natural Resources, Universidad de Castilla-La Mancha, Albacete, Spain
Citations: 1
Access enabled via: An Institution
Abstract
In this chapter, we report a possible alternative use of epigenetics by applying methylation-sensitive amplified fragment length polymorphisms (MS-AFLP) to saffron traceability. Saffron is the most expensive plant-derived product in the world and one
…moreIn this chapter, we report a possible alternative use of epigenetics by applying methylation-sensitive amplified fragment length polymorphisms (MS-AFLP) to saffron traceability. Saffron is the most expensive plant-derived product in the world and one of the most frequently adulterated. One of the most frequent adulteration is by adding to saffron stigmas different parts of the saffron flower itself to increase volumes. While DNA is the same in all the parts of the plant, the epigenetic state can vary according to the organ and/or tissue of origin, making it possible to discriminate the stigmas from the other parts of saffron flower. In the subsequent method, the protocol to carry out a MS-AFLP analysis of saffron DNA methylation patterns is described.
lessFigures (0) & Videos (0)
-
Fig. 1
-
Fig. 2
Experimental Specifications
Techniques
Reagents
Other Keywords
DNA Extraction
Agarose Gel Electrophoresis
Capillary Electrophoresis (CE)
Homogenization
DNA Ligation
Taxonomic DNA Barcoding
Antibodies
Protein
Experimental Models
Kits
Please check back later as we are actively working on gathering the necessary information.
Thank you for your understanding and patience.
Saffron
Epigenetics
Traceability
MS-AFLP
Techniques
DNA Extraction
Agarose Gel Electrophoresis
Capillary Electrophoresis (CE)
Homogenization
DNA Ligation
Taxonomic DNA Barcoding
Reagents
Please check back later as we are actively working on gathering the necessary information.
Thank you for your understanding and patience.
Other Keywords
Saffron
Epigenetics
Traceability
MS-AFLP
Related articles
,
2018,
Springer Protocols
References
- Fernández JA (2004) Biology, biotechnology and biomedicine of saffron. Recent Res Dev Plant Sci 2:127–159
- Food Fraud Database, Decernis Washington. . Accessed on 24 September, 2015https://www.foodfraud.org
- European Saffron White Book. Available online:http://www.europeansaffron.eu/archivos/White book english.pdf
- Fernández JA (2004) Biology, biotechnology and biomedicine of saffron. Recent Res Dev Plant Sci 2:127–159
- Food Fraud Database, Decernis Washington. . Accessed on 24 September, 2015https://www.foodfraud.org
- European Saffron White Book. Available online:http://www.europeansaffron.eu/archivos/White book english.pdf
- Babaei S, Talebi M, Bahar M (2014) Developing an SCAR and ITS reliable multiplex PCR-based assay for safflower adulterant detection in saffron samples. Food Control 35:323–328
- Marieschi M, Torelli A, Bruni R (2012) Quality control of saffron (Crocus sativus L.): development of SCAR markers for the detection of plant adulterants used as bulking agents. J Agric Food Chem 60:10,998–11,004
- Soffritti G, Busconi M, Sánchez RA, Thiercelin JM, Polissiou M, Roldán M, Fernández JA (2016) Genetic and epigenetic approaches for the possible detection of adulteration and auto-adulteration in saffron (Crocus sativus L.) spice. Molecules 21:343
- Busconi M, Colli L, Sánchez RA, Santaella M, De-Los-Mozos Pascual M, Santana O, Roldán M, Fernández JA (2015) AFLP and MS-AFLP analysis of the variation within Saffron Crocus (Crocus sativus L.) Germplasm. PLoS One 10:e0123434
- Widman N, Feng S, Jacobsen SE, Pellegrini M (2014) Epigenetic differences between shoots and roots in Arabidopsis reveals tissue-specific regulation. Epigenetics 9:236–242
- Busconi M, Soffritti G, Stagnati L, Marocco A, Marcos Martínez J, De Los Mozos Pascual M, Fernandez JA (2018) Epigenetic stability in Saffron (Crocus sativus L.) accessions during four consutive years of cultivation and vegetative propagation under open field conditions. Plant Sci 277:1–10
- Lu G, Wu X, Chen B, Gao G, Xu K (2007) Evaluation of genetic and epigenetic modification in rapeseed (Brassica napus) induced by salt stress. J Integr Plant Biol 49:1599–1607
- Marconi G, Pace R, Traini A, Raggi L, Lutts S, Chiusano M et al (2013) Use of MSAP markers to analyse the effects of salt stress on DNA methylation in rapeseed (Brassica napus var. oleifera). PLoS One 8:e75597
- Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23:4407–4414
- Fulneček J, Kovařík A (2014) How to interpret methylation sensitive amplified polymorphism (MSAP) profiles? BMC Genet 15:2
- Fazekas AJ, Kuzmina ML, Newmaster SG, Hollingsworth PM (2012) DNA barcoding methods for land plants. In: Kress WJ, Erickson DL (eds) DNA barcodes: methods and protocols, methods in molecular biology, 1st edn. Wiley, Weinheim
- Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, New York
Abstract
In this chapter, we report a possible alternative use of epigenetics by applying methylation-sensitive amplified fragment length polymorphisms (MS-AFLP) to saffron traceability. Saffron is the most expensive plant-derived product in the world and one
…moreIn this chapter, we report a possible alternative use of epigenetics by applying methylation-sensitive amplified fragment length polymorphisms (MS-AFLP) to saffron traceability. Saffron is the most expensive plant-derived product in the world and one of the most frequently adulterated. One of the most frequent adulteration is by adding to saffron stigmas different parts of the saffron flower itself to increase volumes. While DNA is the same in all the parts of the plant, the epigenetic state can vary according to the organ and/or tissue of origin, making it possible to discriminate the stigmas from the other parts of saffron flower. In the subsequent method, the protocol to carry out a MS-AFLP analysis of saffron DNA methylation patterns is described.
lessExperimental Specifications
Techniques
Reagents
Other Keywords
DNA Extraction
Agarose Gel Electrophoresis
Capillary Electrophoresis (CE)
Homogenization
DNA Ligation
Taxonomic DNA Barcoding
Antibodies
Protein
Experimental Models
Kits
Please check back later as we are actively working on gathering the necessary information.
Thank you for your understanding and patience.
Saffron
Epigenetics
Traceability
MS-AFLP
Techniques
DNA Extraction
Agarose Gel Electrophoresis
Capillary Electrophoresis (CE)
Homogenization
DNA Ligation
Taxonomic DNA Barcoding
Reagents
Please check back later as we are actively working on gathering the necessary information.
Thank you for your understanding and patience.
Other Keywords
Saffron
Epigenetics
Traceability
MS-AFLP
Related articles
Based on techniques
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Detection of RB1 Gene Copy Number Variations Using a Multiplex Ligation-Dependent Probe Amplification Method
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Detection of RB1 Gene Copy Number Variations Using a Multiplex Ligation-Dependent Probe Amplification Method
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References
- Fernández JA (2004) Biology, biotechnology and biomedicine of saffron. Recent Res Dev Plant Sci 2:127–159
- Food Fraud Database, Decernis Washington. . Accessed on 24 September, 2015https://www.foodfraud.org
- European Saffron White Book. Available online:http://www.europeansaffron.eu/archivos/White book english.pdf
- Fernández JA (2004) Biology, biotechnology and biomedicine of saffron. Recent Res Dev Plant Sci 2:127–159
- Food Fraud Database, Decernis Washington. . Accessed on 24 September, 2015https://www.foodfraud.org
- European Saffron White Book. Available online:http://www.europeansaffron.eu/archivos/White book english.pdf
- Babaei S, Talebi M, Bahar M (2014) Developing an SCAR and ITS reliable multiplex PCR-based assay for safflower adulterant detection in saffron samples. Food Control 35:323–328
- Marieschi M, Torelli A, Bruni R (2012) Quality control of saffron (Crocus sativus L.): development of SCAR markers for the detection of plant adulterants used as bulking agents. J Agric Food Chem 60:10,998–11,004
- Soffritti G, Busconi M, Sánchez RA, Thiercelin JM, Polissiou M, Roldán M, Fernández JA (2016) Genetic and epigenetic approaches for the possible detection of adulteration and auto-adulteration in saffron (Crocus sativus L.) spice. Molecules 21:343
- Busconi M, Colli L, Sánchez RA, Santaella M, De-Los-Mozos Pascual M, Santana O, Roldán M, Fernández JA (2015) AFLP and MS-AFLP analysis of the variation within Saffron Crocus (Crocus sativus L.) Germplasm. PLoS One 10:e0123434
- Widman N, Feng S, Jacobsen SE, Pellegrini M (2014) Epigenetic differences between shoots and roots in Arabidopsis reveals tissue-specific regulation. Epigenetics 9:236–242
- Busconi M, Soffritti G, Stagnati L, Marocco A, Marcos Martínez J, De Los Mozos Pascual M, Fernandez JA (2018) Epigenetic stability in Saffron (Crocus sativus L.) accessions during four consutive years of cultivation and vegetative propagation under open field conditions. Plant Sci 277:1–10
- Lu G, Wu X, Chen B, Gao G, Xu K (2007) Evaluation of genetic and epigenetic modification in rapeseed (Brassica napus) induced by salt stress. J Integr Plant Biol 49:1599–1607
- Marconi G, Pace R, Traini A, Raggi L, Lutts S, Chiusano M et al (2013) Use of MSAP markers to analyse the effects of salt stress on DNA methylation in rapeseed (Brassica napus var. oleifera). PLoS One 8:e75597
- Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23:4407–4414
- Fulneček J, Kovařík A (2014) How to interpret methylation sensitive amplified polymorphism (MSAP) profiles? BMC Genet 15:2
- Fazekas AJ, Kuzmina ML, Newmaster SG, Hollingsworth PM (2012) DNA barcoding methods for land plants. In: Kress WJ, Erickson DL (eds) DNA barcodes: methods and protocols, methods in molecular biology, 1st edn. Wiley, Weinheim
- Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, New York
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Fig. 1
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