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Exopolysaccharide from surface-liquid culture of Clonostachys rosea originates from autolysis of the biomass

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Abstract

We describe the purification and chemical characterization of galactomannans that appear both in the biomass and the culture broth during surface-liquid culture of the fungus Clonostachys rosea, a common facultative saprophyte that has potential to be used as a biological control agent against several plant pathogenic fungi, insects and nematodes. The galactomannans from both sources had comparable ratios of Man, Gal and Glc and the similarity were confirmed by 1H, 13C NMR, HMQC, and COSY spectra. We propose that the galactomannan in the culture broth originates from autolysis of the biomass, based not only on the similarity that it has with the galactomannan extracted from the biomass but also on the fact that its concentration increased rapidly after glucose depletion from the medium, when biomass concentration was falling. Polysaccharides from C. rosea have not previously been characterized; we show that the characteristics of the galactomannans are consistent with those that have been reported for other members of the Bionectriaceae, the family to which C. rosea belongs.

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Acknowledgments

The work was supported financially by PRONEX-Carboidratos and CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brasil), a Brazilian government agency for the advancement of science and technology. Research scholarships were granted to Graciele Viccini, Thalita Romano Martinelli, Rodrigo Otávio de Faria, Guilherme Lanzi Sassaki, and David Alexander Mitchell by CNPq and to Elaine Rosechrer Carbonero by CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brazil).

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  1. Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Cx.P. 19046 Centro Politécnico, Curitiba, Paraná, 81531-980, Brazil

    Graciele Viccini, Thalita Romano Martinelli, Regielly Caroline Raimundo Cognialli, Rodrigo Otávio de Faria, Elaine Rosechrer Carbonero, Guilherme Lanzi Sassaki & David Alexander Mitchell

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  1. Graciele Viccini
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  2. Thalita Romano Martinelli
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  3. Regielly Caroline Raimundo Cognialli
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  6. Guilherme Lanzi Sassaki
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Correspondence to David Alexander Mitchell.

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Communicated by Geoffrey Turner.

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Viccini, G., Martinelli, T.R., Cognialli, R.C.R. et al. Exopolysaccharide from surface-liquid culture of Clonostachys rosea originates from autolysis of the biomass. Arch Microbiol 191, 369–378 (2009). https://doi.org/10.1007/s00203-009-0464-0

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