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Phomopsis liquidambaris reduces ethylene biosynthesis in rice under salt stress via inhibiting the activity of 1-aminocyclopropane-1-carboxylate deaminase

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A Correction to this article was published on 14 March 2022

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Abstract

The endophytic fungus Phomopsis liquidambaris is characterized as a plant growth-promoting agent under salt stress, but its mechanism is unknown. Herein, 1-aminocyclopropane-1-carboxylate deaminase (ACCD) from the strain was confirmed that it had the ability of utilizing 1-aminocyclopropane-1-carboxylate as the sole nitrogen source. The full-length ACCD gene was 1152 bp, which encodes a mature protein of 384 amino acids with a molecular mass of 41.53 kDa. The ACCD activity was 3.9-fold in 3 mmol L−1 ACC by qRT-PCR under salt stress comparing with no salt tress. Ethylene production was increased to 34.55–70.60% and reduced the growth of rice by 23–69.73% under salt stress. Inoculation of P. liquidambaris increased root-shoot length, fresh and dry weight, and overall growth of stressed rice seedlings. ACC accumulation, ACC synthase and ACC oxidase activities increased in salt-treated rice seedlings, while they were significantly reduced when P. liquidambaris was inoculated into rice by qRT-PCR. It therefore can be concluded that P. liquidambaris can be used as a plant growth promoting fungus against salt stress and other biotic or abiotic stresses.

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Abbreviations

ACC:

1-Aminocyclopropane-1-carboxylic acid

ACCD:

ACC deaminase

ACS:

ACC synthase

ACO:

ACC oxidase

SAM:

S-adenosyl methionine

AVG:

Aminoethoxyvinylglycine

PGPM:

Plant growth promoting microorganisms

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Acknowledgements

We thank Miss Xiao-Mi Wang from Nanjing Institute of Soil Science, Academy of Science (CAS) for her technical assistance and her help in measuring ethylene produced by salt-stressed rice seedlings using GC. We are grateful to the National Natural Science Foundation of China (31570491), a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions, Research Fund of State Key Laboratory of Soil and Sustainable Agriculture, Nanjing Institute of Soil Science, Chinese Academy of Science (Y412201435) for their financial support.

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Authors and Affiliations

  1. Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, College of Life Sciences, Nanjing Normal University, Nanjing, 210023, Jiangsu, China

    M. A. Siddikee, M. I. Zereen, Mei Wu, Wei Zhang & Chuan-Chao Dai

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  1. M. A. Siddikee
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  2. M. I. Zereen
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  3. Mei Wu
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  4. Wei Zhang
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  5. Chuan-Chao Dai
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Contributions

CCD and MAS designed the experiment. MAS, MIZ, MW and WZ conducted the study, processed the data, performed data analysis, and wrote the paper. All authors discussed the results and commented on the manuscript.

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Correspondence to Chuan-Chao Dai.

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Communicated by Erko Stackebrandt.

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Siddikee, M.A., Zereen, M.I., Wu, M. et al. Phomopsis liquidambaris reduces ethylene biosynthesis in rice under salt stress via inhibiting the activity of 1-aminocyclopropane-1-carboxylate deaminase. Arch Microbiol 203, 6215–6229 (2021). https://doi.org/10.1007/s00203-021-02588-w

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