Abstract
In the present work, Amycolatopsis sp. SND-1 (SND-1) was isolated from Cleome chellidonii Linn. (C. chellidonii) was performed as biocontrol and resistance elicitor in Vigna radiata (L.) R. Wilczek (mung bean) plants against Cercospora leaf spot causing pathogen Cercospora canescens (C. canescens). The SND-1 isolate showed 74% of inhibition against C. canescens in dual culture and GC–MS analysis revealed the presence of antifungal compounds. Molecular characterization through 16S rRNA showed that the isolated SND-1 belongs to Amycolatopsis sp. The in vitro plant growth trials exhibited production of indole acetic acid, gibberellic acid, cytokinin, ammonia, hydrogen cyanide, and siderophore and phosphate solubilization. In vivo study with talcum formulation of SND-1 revealed a significant increase in plant root length, shoots length, root and shoot fresh weight, and reduced the disease severity in treated mung bean plants. Triggering of resistance by SND-1 formulation was studied by histochemical depositions and biochemical defense enzymes that resulted in the acceleration in defense response in comparison with control plants. The bioactive endophytic Amycolatopsis sp. SND-1 enhanced the defense against C. canescens infection; hence, it can be used as a biological control agent in mung bean cultivars.
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The authors are grateful to the Department of PG Studies in Botany, University Scientific Instrumentation Centre (USIC), and Sophisticated Analytical Instrumentation Facility (SAIF) for extending the laboratory and instrumentation facility Karnatak University Dharwad, Karnataka, India.
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The concept of the present research was designed and supervised by SN; all the experiments were performed by DSB. RSK helped in conceptualization, data validation, and initial manuscript preparation along with SN and DSB. The methodology, formal analysis, editing, and original draft were prepared by DSB, and final manuscript was constructed by SN along with RSK.
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Basavarajappa, D.S., Kumar, R.S. & Nayaka, S. Formulation-based antagonistic endophyte Amycolatopsis sp. SND-1 triggers defense response in Vigna radiata (L.) R. Wilczek. (Mung bean) against Cercospora leaf spot disease. Arch Microbiol 205, 77 (2023). https://doi.org/10.1007/s00203-023-03419-w
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DOI: https://doi.org/10.1007/s00203-023-03419-w