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Phylogenetic positions of Mn2+-oxidizing bacteria and fungi isolated from Mn nodules in rice field subsoils

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Abstract

We isolated manganous ion (Mn2+) oxidizing bacteria and fungi from Mn nodules collected from two Japanese rice fields. The phylogenetic position of the Mn-oxidizing bacteria and fungi was determined based on their 16S rDNA and 18S rDNA sequences, respectively. Among 39 bacterial and 25 fungal isolates, Burkholderia and Acremonium strains were the most common and dominant Mn2+-oxidizing bacteria and fungi, respectively. Majority of the Mn-oxidizing bacteria and fungi isolated from the Mn nodules belonged to the genera that had been isolated earlier from various environments. Manganese oxide depositions on Mn2+-containing agar media by these microorganisms proceeded after their colony developments, indicating that the energy produced from Mn2+ oxidation is poorly used for microbial growth.

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Acknowledgments

This study was supported by the JSPS Postdoctoral Fellowship Program for Foreign Researchers and the JSPS Grant-in-Aid for Scientific Research. We thank Dr. Takeshi Watanabe and Dr. Natsuko Nakayama in our laboratory for their help in collection of samples.

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Authors and Affiliations

  1. Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 464-8601, Japan

    Vita Ratri Cahyani, Jun Murase, Susumu Asakawa & Makoto Kimura

  2. Agricultural Experiment Station, Okayama Prefectural General Agricultural Center, Okayama, 709-0801, Japan

    Eiji Ishibashi

  3. Faculty of Agriculture, Sebelas Maret University, Surakarta, 57126, Indonesia

    Vita Ratri Cahyani

  4. Laboratory of Soil Biology and Chemistry, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 464-8601, Japan

    Makoto Kimura

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  1. Vita Ratri Cahyani
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Correspondence to Makoto Kimura.

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Cahyani, V.R., Murase, J., Ishibashi, E. et al. Phylogenetic positions of Mn2+-oxidizing bacteria and fungi isolated from Mn nodules in rice field subsoils. Biol Fertil Soils 45, 337–346 (2009). https://doi.org/10.1007/s00374-008-0337-8

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  • DOI: https://doi.org/10.1007/s00374-008-0337-8

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