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Repeated shock stress facilitates basolateral amygdala synaptic plasticity through decreased cAMP-specific phosphodiesterase type IV (PDE4) expression

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Abstract

Previous studies have shown that exposure to stressful events can enhance fear memory and anxiety-like behavior as well as increase synaptic plasticity in the rat basolateral amygdala (BLA). We have evidence that repeated unpredictable shock stress (USS) elicits a long-lasting increase in anxiety-like behavior in rats, but the cellular mechanisms mediating this response remain unclear. Evidence from recent morphological studies suggests that alterations in the dendritic arbor or spine density of BLA principal neurons may underlie stress-induced anxiety behavior. Recently, we have shown that the induction of long-term potentiation (LTP) in BLA principal neurons is dependent on activation of postsynaptic D1 dopamine receptors and the subsequent activation of the cyclic adenosine 5′-monophosphate (cAMP)—protein kinase A (PKA) signaling cascade. Here, we have used in vitro whole-cell patch-clamp recording from BLA principal neurons to investigate the long-term consequences of USS on their morphological properties and synaptic plasticity. We provided evidence that the enhanced anxiety-like behavior in response to USS was not associated with any significant change in the morphological properties of BLA principal neurons, but was associated with a changed frequency dependence of synaptic plasticity, lowered LTP induction threshold, and reduced expression of phosphodiesterase type 4 enzymes (PDE4s). Furthermore, pharmacological inhibition of PDE4 activity with rolipram mimics the effects of chronic stress on LTP induction threshold and baseline startle. Our results provide the first evidence that stress both enhances anxiety-like behavior and facilitates synaptic plasticity in the amygdala through a common mechanism of PDE4-mediated disinhibition of cAMP-PKA signaling.

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  1. Steve Ryan and Chenchen Li contributed equally to this work.

Authors and Affiliations

  1. Behavioral Neuroscience and Psychiatric Disorders, Yerkes National Primate Research Center, Atlanta, GA, USA

    Steve Ryan, Chenchen Li, Aurélie Menigoz, Katelyn Gordon & Donald G. Rainnie

  2. Department of Psychiatry, Emory University School of Medicine, Atlanta, GA, USA

    Steve Ryan, Chenchen Li, Aurélie Menigoz & Donald G. Rainnie

  3. Department of Medicine, Center for Translational and International Hematology, Vascular Medicine Institute, University of Pittsburgh, Pittsburgh, PA, USA

    Rimi Hazra

  4. Department of Cellular and Molecular Pharmacology, Chicago Medical School, Rosalind Franklin University of Medicine and Science, North Chicago, USA

    Joanna Dabrowska

  5. Department of Neuroscience, Chicago Medical School, Rosalind Franklin University of Medicine and Science, North Chicago, USA

    Joanna Dabrowska

  6. Department of Neuroscience and Physiology, Neuroscience Institute, NYU Langone Medical Center, New York, NY, USA

    David Ehrlich

  7. Department of Otolaryngology, NYU Langone School of Medicine, New York, NY, USA

    David Ehrlich

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Correspondence to Donald G. Rainnie.

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This work was supported by funding from the National Institute of Mental Health, Grant MH069852 to DGR.

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Ryan, S., Li, C., Menigoz, A. et al. Repeated shock stress facilitates basolateral amygdala synaptic plasticity through decreased cAMP-specific phosphodiesterase type IV (PDE4) expression. Brain Struct Funct 223, 1731–1745 (2018). https://doi.org/10.1007/s00429-017-1575-z

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