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Enhanced laccase production by mutagenized Myrothecium verrucaria using corn stover as a carbon source and its potential in the degradation of 2-chlorophen

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Abstract

Chlorophenols are widely used in industry and are known environmental pollutants. The degradation of chlorophenols is important for environmental remediation. In this study, we evaluated the biodegradation of 2-chlorophenol using crude laccase produced by Myrothecium verrucaria. Atmospheric and room temperature plasma technology was used to increase laccase production. The culture conditions of the M-6 mutant were optimized. Our results showed that corn stover could replace glucose as a carbon source and promote laccase production. The maximum laccase activity of 30.08 U/mL was achieved after optimization, which was a 19.04-fold increase. The biodegradation rate of 2-chlorophenol using crude laccase was 97.13%, a positive correlation was determined between laccase activity and degradation rate. The toxicity of 2-CP was substantially reduced after degradation by laccase solution. Our findings show the feasibility of the use of corn stover in laccase production by M. verrucaria mutant and the subsequent biodegradation of 2-chlorophenol using crude laccase.

Highlights

  • A mutant of M. verrucaria with high laccase production and genetic stability was obtained using ARTP mutagenesis.

  • Culture conditions were optimized, and extracellular laccase activity was increased by corn stover and copper ion supplementation.

  • Crude laccase could degrade 2-chlorophenol by up to 97.13%.

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Data availability statement

All data generated or analyzed during this study are included in this published article.

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Funding

This work was supported by Jilin Scientific and Technological Development Program, Grant/Award number: 20210101036JC and National College Students Innovation and Entrepreneurship Project in China.

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  1. Ze-Chang Gou and Min-Jie Lu contributed equally to this work.

Authors and Affiliations

  1. College of Life Sciences, Jilin Agricultural University, Changchun, 130118, Jilin, China

    Ze-Chang Gou, Min-Jie Lu, Xiao-Yu Cui, Mei-Yi Jiang, Ya-Shuo Wang, Zi-Qi Wang, Xiao-Xiao Yu, Shan-Shan Tang, Guang Chen & Ying-Jie Su

  2. Key Laboratory of Straw Comprehensive Utilization and Black Soil Conservation, Ministry of Education, Changchun, 130118, Jilin, China

    Ze-Chang Gou, Min-Jie Lu, Xiao-Yu Cui, Mei-Yi Jiang, Ya-Shuo Wang, Zi-Qi Wang, Xiao-Xiao Yu, Shan-Shan Tang, Guang Chen & Ying-Jie Su

  3. College of Food Science Technology and Chemical Engineering, Hubei University of Arts and Science, Xiangyang, 441000, Hubei, China

    Xi-Qing Wang

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Contributions

ZCG and MJL wrote the manuscript; XQW and YSW conducted experiments; ZQW and MYJ contributed new reagents or analytical tools; XYC, XXY and SST analyzed data; GC and YJS conceived and designed the experiments. All authors have read and approved the final manuscript.

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Correspondence to Ying-Jie Su.

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Gou, ZC., Lu, MJ., Cui, XY. et al. Enhanced laccase production by mutagenized Myrothecium verrucaria using corn stover as a carbon source and its potential in the degradation of 2-chlorophen. Bioprocess Biosyst Eng 45, 1581–1593 (2022). https://doi.org/10.1007/s00449-022-02767-z

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