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Change in biochemical parameters of Persian oak (Quercus brantii Lindl.) seedlings inoculated by pathogens of charcoal disease under water deficit conditions

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Drought alone or in combination with charcoal disease pathogens led to modifications in some biochemical characteristics of Persian oak seedlings. Drought conditions enhances the effects of charcoal fungus with effects at biochemical level.

Abstract

Charcoal disease is one of the common diseases in oak forest of Zagros in western Iran that has increased in the recent years. The disease is associated with abiotic stress, especially drought, and contributes to the decline of Persian oak (Quercus brantii Lindl.), the major oak species in this forest association. Persian oak seedlings were exposed to a factorial combination of two irrigation levels and inoculation with either Biscogniauxia mediterranea or Obolarina persica (agents of charcoal disease). Proline, total soluble sugar and soluble protein contents in seedling foliage were increased in response to charcoal pathogen inoculation, especially when combined with water stress, while starch, chlorophyll a + b and carotenoid contents strongly declined. Pathogen inoculation stimulated malondialdehyde content, electrolyte leakage, and hydrogen peroxide and superoxide radical contents in oak leaves, and were increased by water stress. The combination of charcoal disease agents and water stress increased peroxidase and superoxide dismutase activity while, for catalase and ascorbate peroxidase activities, the interaction between the two factors of variability was not significantly increased. Ascorbate peroxidase activity was at maximum level in seedlings infected with O. persica independently of water stress. Glutathione reductase had the highest activity in inoculated seedlings under soil moisture stress while those under higher soil moisture levels, the enzyme was increased only when inoculated with O. persica. Contents of non-enzymatic antioxidants including ascorbic acid and glutathione increased in response to combined pathogen inoculation and water stress and was higher when seedlings were inoculated with O. persica than with B. mediterranea. Chitinase activity significantly increased because of pathogen inoculation. Furthermore, foliar phenylalanine ammonia lyase activity was higher under all treatment combinations, as compared to the controls. The effects of B. mediterranea or O. persica pathogens on foliar biochemical responses of Q. brantii seedlings were more severe under water-stress and it was more sensitive to B. mediterranea than O. persica.

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Acknowledgements

We do acknowledge the helps in the greenhouse by Dr. Saham Mirzaei Tork. We gratefully acknowledge the technical assistance of Dr. Mehrdad Zarafshar and Dr. Mohammad Khezry for all their support in the laboratory.

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Authors and Affiliations

  1. Department of Forestry, Faculty of Natural Resources, Tarbiat Modares University, POB 14115-111, Tehran, Iran

    Ehsan Ghanbary & Masoud Tabari Kouchaksaraei

  2. Department of Agriculture, Food and Environment, University of Pisa, 56124, Pisa, Italy

    Lucia Guidi

  3. Iranian Plant Protection Research Institute, POB 1454, Tehran, Iran

    Mansoureh Mirabolfathy

  4. Department of Forestry, Faculty of Natural Resources, Tehran University, POB 41111, Karaj, Iran

    Vahid Etemad

  5. Department of Agronomy, Faculty of Agriculture, Tarbiat Modares University, POB 14115-336, Tehran, Iran

    Seyed Ali Mohammad Modarres Sanavi

  6. Department of Horticulture and Crop Science, Ohio State University, Columbus, OH, 43210, USA

    Daniel Struve

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Ghanbary, E., Kouchaksaraei, M.T., Guidi, L. et al. Change in biochemical parameters of Persian oak (Quercus brantii Lindl.) seedlings inoculated by pathogens of charcoal disease under water deficit conditions. Trees 32, 1595–1608 (2018). https://doi.org/10.1007/s00468-018-1736-6

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