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Real-time PCR for differential determination of the tomato wilt fungus, Fusarium oxysporum f. sp. lycopersici, and its races

  • Fungal Diseases
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Abstract

Five primer/probe sets to identify the tomato wilt pathogen, Fusarium oxysporum f. sp. lycopersici (FOL), and its three races selectively were designed based on the rDNA-intergenic spacer and avirulence genes. Real-time PCR using genomic DNA from mycelia and soil DNA with the primer/probe sets allowed the successful identification of FOL and its races.

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Acknowledgments

We thank Yuji Hosobuchi (Sakata Seed, Kimitsu, Japan), Hideaki Tateishi (Kureha, Iwaki, Japan), Taiji Miyake (Kureha), Ken Watanabe (Ibaraki Agricultural Center, Kasama, Japan), Suminaga Suwa (Gunma prefecture, Gunma, Japan), Yoshimiki Amemiya (Chiba University, Matsudo, Japan), and Fujio Kodama (previously at Hokkaido Central Agricultural Experiment Station, Naganuma, Japan) for providing fungal isolates. This study was partly supported by eDNA Project (Development of soil diversity analysis system with environmental DNA) of the Ministry of Agriculture, Forestry, and Fisheries (MAFF), and Grants-in-Aid (16405021, 18380030) from The Japan Society for the Promotion of Sciences (JSPS) for TA.

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Authors and Affiliations

  1. Laboratory of Plant Pathology, Tokyo University of Agriculture and Technology (TUAT), Fuchu, Tokyo, 183-8509, Japan

    Keigo Inami, Chizu Yoshioka, Tohru Teraoka & Tsutomu Arie

  2. Saitama Prefectural Agriculture and Forestry Research Center, Kuki, Saitama, Japan

    Yasushi Hirano

  3. National Agricultural Research Center (NARC), Tsukuba, Ibaraki, Japan

    Masato Kawabe

  4. National Institute for Agro-Environmental Sciences (NIAS), Tsukuba, Ibaraki, Japan

    Seiya Tsushima

Authors
  1. Keigo Inami
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  2. Chizu Yoshioka
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  3. Yasushi Hirano
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  4. Masato Kawabe
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  5. Seiya Tsushima
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  6. Tohru Teraoka
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  7. Tsutomu Arie
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Corresponding author

Correspondence to Tsutomu Arie.

Additional information

K. Inami and C. Yoshioka have contributed equally to this work.

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Inami, K., Yoshioka, C., Hirano, Y. et al. Real-time PCR for differential determination of the tomato wilt fungus, Fusarium oxysporum f. sp. lycopersici, and its races. J Gen Plant Pathol 76, 116–121 (2010). https://doi.org/10.1007/s10327-010-0224-7

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  • DOI: https://doi.org/10.1007/s10327-010-0224-7

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