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Phylogenetic diversity of Calonectria ilicicola causing Cylindrocladium black rot of peanut and red crown rot of soybean in southern China

  • Fungal Diseases
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Abstract

Calonectria ilicicola Boedijin & Reitsma (anamorph: Cylindrocladium parasiticum Crous, Wingfield & Alfennas) is an important pathogen worldwide, which causes Cylindrocladium black rot (CBR) in peanut (Arachis hypogaea L.) and red crown rot (RCR) in soybean [Glycine max (L.) Merr.]. We isolated the CBR and RCR pathogens from heavily diseased peanut and soybean fields in southern China and assessed their pathogenicity. Two inoculation methods were applied separately to evaluate the pathogenicity of different C. ilicicola strains on peanut cultivar Yueyou 13. Our results indicate that the Chinese C. ilicicola strains exhibited a range of virulence on peanut, with strains of soybean origin exhibiting a weak virulence relative to strains isolated from peanut. Multilocus sequence type analysis indicates that the C. ilicicola strains partitioned into two distinct clades, which were heavily structured based on geographical origin. Phylogenetic results demonstrated that the origins of C. ilicicola in southern China were multiple. This study also revealed that the backgrounds of CBR pathogens may be different from those of RCR pathogens.

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Acknowledgements

We thank Prof. H. Liao, Root Biology Center of South China Agricultural University for technical support. We also thank Prof. Pedro W. Crous of the CBS Fungal Biodiversity Centre of the Netherlands, Prof. J. Xu of McMaster University of Canada and Prof. K. R. Chung of National Chung Hsing University of Taiwan, for valuable comments on the manuscript. In particular, we thank Brendan K. Riely (University of California-Davis) for his great and valuable contributions in revising most of the issues in our manuscript. This study was supported by the Program for the Agricultural Research of Guangdong Province (2009B020310014) and National Key Basic Research Funds of China (Grant No. 2005CB120902).

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Authors and Affiliations

  1. Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou, 510642, People’s Republic of China

    Yunpeng Gai, Qiaowen Deng, Xinglong Chen, Mingfang Guan, Xiang Xiao, Dagao Xu & Ruqian Pan

  2. Institute of Biotechnology, Zhejiang University, Hangzhou, 310058, Zhejiang, People’s Republic of China

    Yunpeng Gai

  3. Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, People’s Republic of China

    Mingguang Deng

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  1. Yunpeng Gai
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Correspondence to Yunpeng Gai or Ruqian Pan.

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Gai, Y., Deng, Q., Chen, X. et al. Phylogenetic diversity of Calonectria ilicicola causing Cylindrocladium black rot of peanut and red crown rot of soybean in southern China. J Gen Plant Pathol 83, 273–282 (2017). https://doi.org/10.1007/s10327-017-0724-9

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