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Ecophysiology of Alternaria alternata Causing Diseases in Litchi (Litchi chinensis)

Ökophysiologische Eigenschaften des Krankheitserregers Alternaria alternata an Litschi (Litchi chinensis)

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Abstract

Alternaria alternata (Fr.) Keissl. is an important pathogen of litchi (Litchi chinensis Sonn.) that causes blight of leaf, panicle and fruits. Ecophysiological determinants favouring growth and sporulation are keys to understand the pathogen behaviour. Therefore, studies were conducted to assess the effect of different medium, carbon source, pH, temperature, relative humidity and exposure to UV radiation on growth and sporulation of A. alternata under in vitro conditions. Results showed that the maximum mycelia growth rate was on Host Leaf Extract Dextrose Agar but sporulation was the maximum on Potato Dextrose Agar medium. The mycelia growth was more with dextrose as carbon source but the maximum dry weight was recorded in maltose amended medium. Optimum pH for mycelia growth was 6.0, and below or above this pH, mycelia growth was slow. It was evident that the maximum mycelia growth rate and germination of conidia were at 30 °C. The thermal death point of A. alternata was found to be 45 °C with 10 min. exposure. The maximum mean growth rate and germination percent was recorded at 100% relative humidity. The growth rate decreased with increasing exposure time to UV‑B rays up to 20 min, but at exposure time 25 and 30 min, colony changed to profuse mycelia growth with smaller sporulating zone in the centre compared to untreated dishes. The findings of the study will be helpful in understanding epidemiology and devising management strategies of this disease.

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References

  • Awadhiya GK (1991) Effect of some cultural factors on growth and spore germination of Alternaria carthami. J Oilseed Res 8(1):123–125

    Google Scholar 

  • Awasthi DP, Sarkar S, Mishra NK, Kaiser SAKM (2005) Disease situation of some major fruit crops in new alluvial plains of West Bengal. Environ Ecol 23:497–499

    Google Scholar 

  • Balai LP, Ahir RR (2013) Role of temperature and relative humidity on mycelial growth of Alternaria alternata infecting brinjal. Trends Biosci 6:307–308

    Google Scholar 

  • Chen LJ, Way Y, Feng GQ (2000) Study on the germination characteristics of conidia of Alternaria tenuissima. Plant Prot 26(5):24–25

    Google Scholar 

  • Deivendran P (2009) Eco-friendly management of onion (Allium cepa L.) leaf blight disease caused by Alternaria palandui. M.Sc.(Ag.) Thesis. Tamil Nadu Agricultural University, Coimbatore

    Google Scholar 

  • Devi PA, Mohan S, Kalieswari N, Maharaja N (2014) Physiological and nutrition requirements for the determination of Alternaria helianthi in sunflower. World J Agric Res 2(4):47–52

    Google Scholar 

  • FAO (2002) Lychee production in the Asia-Pacific region. RAP Publication (FAO), Bangkok (Thailand)

    Google Scholar 

  • Fourtouni A, Manetas Y, Christias C (1998) Effects of UV‑B radiation on growth, pigmentation, and spore production in the phytopathogenic fungus Alternaria solani. Can J Bot 76(12):2093–2099

    CAS  Google Scholar 

  • Garibaldi A, Gilardi G, Gullino ML (2007) First report of Alternaria leaf spot on Camellia in Italy. Plant Dis 91(3):324

    Article  CAS  PubMed  Google Scholar 

  • Gawai DU, Mangnalikar SS (2018) Effect of temperature and pH on growth of Alternaria alternata, leaf spot pathogen of soyabean. Biosci Discov 9(1):162–165

    Google Scholar 

  • Gupta RBL, Pathak VN, Verma OP (1985) Physical factors in relation to conidial germination in Alternaria porri the incitant of purple blotch of onion. Korean J Plant Prot 24(3):135–139

    Google Scholar 

  • Gveroska B, Jovancev P (2011) Biological characteristics of Alternaria alternata in tobacco. Тутун/Tobacco 61(1–6):41–48

    Google Scholar 

  • Hubballi M, Nakkeeran S, Raguchander T, Anand T, Samiyappan R (2010) Effect of environmental condition on growth of Alternaria alternata causing leaf blight of noni. World J Agric Res 6(2):171–177

    Google Scholar 

  • Hubballi M, Nakkeeran S, Raguchander T, Anand T, Renukadevi P (2011) Physiological characterization of Colletotrichum gloeosporioides, the incitant of anthracnose disease of noni in India. Arch Phytopathol Plant Prot 44(11):1105–1114

    Article  Google Scholar 

  • Kamalalakshmi C (1996) Studies on leaf blight disease of Jathimalli Jasminum grandiflorum L. incited by Alternaria alternata (Fr.) Keissler. M.Sc.(Ag.) Thesis. Tamil Nadu Agricultural University, Coimbatore

    Google Scholar 

  • Kannan K, Mohan S (2010) In vitro evaluation of plant oils and oil cakes against Alternaria alternata causing leaf spot disease on Aloe vera. South Indian Hortic 58:301–303

    Google Scholar 

  • Kumar V, Anal AKD (2018) Incidence and severity of leaf, panicle and fruit blights of litchi caused by Alternaria alternata. Indian Phytopathol 71(1):153–157

    Article  Google Scholar 

  • Kumar V, Kumar A, Nath V (2011) Emerging pests and diseases of litchi (Litchi chinensis Sonn.). Pest Manag Hortic Ecosyst 17:11–13

    Google Scholar 

  • Kumar V, Anal AKD, Nath V (2014) Prevalence of some threatening pests and disease of Litchi (Litchi chinensis Sonn.) in Bihar state of India. J Appl Hortic 16(3):235–240

    Article  Google Scholar 

  • Kumar V, Purbey SK, Anal AKD (2016a) Losses in litchi at various stages of supply chain and changes in fruit quality parameters after harvest. Crop Prot 79:97–104

    Article  Google Scholar 

  • Kumar V, Purbey SK, Pongener A, Anal AKD, Nath V (2016b) Effect of some fructoplane antagonists and postharvest dip treatments on litchi fruit rots and shelf life. Int J Trop Agric 64:333–343

    Google Scholar 

  • Kumar V, Anal AKD, Rai S, Nath V (2018) Leaf, panicle and fruit blight of litchi (Litchi chinensis) caused by Alternaria alternata in Bihar state, India. Can J Plant Pathol 40(1):84–89

    Article  Google Scholar 

  • Madhavi M, Kavitha A, Vijayalakshmi M (2012) Studies on Alternaria porri (Ellis) Ciferri pathogenic to onion (Allium cepa L.). Arch Appl Sci Res 4(1):1–9

    Google Scholar 

  • Martin JE, Fernandez HS (2006) First report of Alternaria brown spot of citrus caused by Alternaria alternata in Peru. Plant Dis 90:686

    Article  Google Scholar 

  • Mishra PK, Thawani V (2016) Characterization of Alternaria alternata isolated from Calotropis gigantea plant leaf. Int J Curr Microbiol Appl Sci 5(10):459–466

    Article  Google Scholar 

  • Nagrale DT, Gaikwad AP, Sharma L (2013) Morphological and cultural characterization of Alternaria alternata (Fr.) Keissler blight of gerbera (Gerbera jamesonii H. Bolus ex J.D. Hook). J Appl Nat Sci 5(1):171–178

    Article  Google Scholar 

  • NHB (2017) Horticultural statistics at a glance. http://nhb.gov.in/statistics/Publication/Horticulture%20At%20a%20Glance%202017%20for%20net%20uplod%20(2).pdf. Accessed 17 Jan 2019 (New Delhi (India): Ministry of Agriculture & Farmers Welfare, Government of India, Oxford University)

  • Patil JS, Suryawanshi NS (2015) Effect of nutritional and physiological characters of Alternaria alternata causing fruit rot of strawberry. Bionano Front 8(1):57–64

    Google Scholar 

  • Prasad RD, Kulshrestha DD (1999) Bacterial antagonists of Alternaria helianthi of sunflower. J Mycol Plant Pathol 29:127–128

    Google Scholar 

  • Prasad SS, Bilgrami RS (1974) Investigations on diseases of litchi: VI. Post-harvest diseases of fruit in India. Plant Dis Rep 58:1134–1136

    Google Scholar 

  • Rajmane SD, Korekar SL (2012) Impact of carbon and nitrogen sources on pectinase production of postharvest fungi. Curr Bot 3(3):1–3

    CAS  Google Scholar 

  • Ramjegathesh R (2003) Studies on the eco-friendly management of the onion leaf blight disease caused by Alternaria alternata (Fr.) Keissler. M.Sc. (Ag) Thesis. Tamil Nadu Agricultural University, Coimbatore

    Google Scholar 

  • Ramjegathesh R, Ebenezar EG (2012) Morphological and physiological characters of Alternaria alternata causing leaf blight disease of onion. Int J Plant Pathol 10(7):1–11

    Google Scholar 

  • Sallam NMA (2011) Control of tomato early blight disease by certain aqueous plant extracts. Plant Pathol J 10(4):187–191

    Article  Google Scholar 

  • Sandhu SS (1995) Effect of physical factors on germination of entomopathogenic fungus Beauveria bassiana conidia. Proc Natl Acad Sci Lett 18(1/2):1–5

    Google Scholar 

  • Singh P (2000) Studies on Alternaria porri (Ellis) Ciferri pathogenic to onion (Allium cepa L.). Adv Plant Sci 13:639–641

    Google Scholar 

  • Singh V, Kumar P, Sinha AP (2001) Influence of different media, pH and temperature on growth and sporulation of Alternaria alternata (Fr.) Keissler causing Alternaria blight of chickpea. Legum Res 24(4):238–242

    Google Scholar 

  • Wang Z, Wang CY, Wang YB, Xue JJ, Li Z, Li JJ, Gu LG, Sung CK (2013) Effect of different pH values on growth and sporulation of Estye vermicola. Afr J Microbiol Res 7(25):3217–3221

    Article  Google Scholar 

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Acknowledgements

Authors are grateful to the Director, ICAR-National Research Centre on Litchi, Muzaffarpur, Bihar, India for providing research facilities and necessary support to conduct this study.

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Authors and Affiliations

  1. Amity Institute of Microbial Technology, Amity University, 201 313, Noida, Uttar Pradesh, India

    Ajit Kumar Dubedi Anal & Ajit Varma

  2. ICAR-National Research Centre on Litchi, 842 002, Muzaffarpur, Bihar, India

    Vinod Kumar

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  1. Ajit Kumar Dubedi Anal
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  2. Vinod Kumar
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Correspondence to Vinod Kumar.

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A.K.D. Anal, V. Kumar and A. Varma declare that they have no competing interests.

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Anal, A.K.D., Kumar, V. & Varma, A. Ecophysiology of Alternaria alternata Causing Diseases in Litchi (Litchi chinensis). Erwerbs-Obstbau 63, 163–173 (2021). https://doi.org/10.1007/s10341-021-00554-w

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  • DOI: https://doi.org/10.1007/s10341-021-00554-w

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